SMED30010277

Overview
NameSMED30010277
Smed IDSMED30010277
Length (bp)1951
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010277 (SMED30010277) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010277 (SMED30010277) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010277 (SMED30010277) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010277 (SMED30010277) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010277

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30010277h1SMcG0013305 SmedASXL_014865SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30010277h1SMcG0013305 dd_Smed_v4_8640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30010277h1SMcG0013305 dd_Smed_v4_8640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30010277h1SMcG0013305 dd_Smed_v4_8640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010277h1SMcG0013305 dd_Smed_v4_8640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010277h1SMcG0013305 dd_Smed_v4_8640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30010277h1SMcG0013305 dd_Smed_v4_8640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30010277h1SMcG0013305 dd_Smed_v4_8640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30010277h1SMcG0013305 dd_Smed_v6_8640_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30010277h1SMcG0013305 dd_Smed_v4_8640_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010277 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000034:275950..286384 +9495
Homology
[Close]
BLAST of SMED30010277 vs. Ensembl Human
Match: CCDC34 (coiled-coil domain containing 34 [Source:HGNC Symbol;Acc:HGNC:25079])

HSP 1 Score: 57.3806 bits (137), Expect = 3.861e-8
Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 3 
Query: 1407 SQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPV 1565
            ++  F+ WLE  K    P   SYG ++GKLT +Y   S P PAF+NP PW P+
Sbjct:  272 AEKKFQEWLENAKHKPRPAAKSYGYANGKLTGFYSGNSYPEPAFYNPIPWKPI 324
[Close]
BLAST of SMED30010277 vs. Ensembl Xenopus
Match: cdc73 (cell division cycle 73 [Source:Xenbase;Acc:XB-GENE-949345])

HSP 1 Score: 54.299 bits (129), Expect = 3.447e-7
Identity = 24/47 (51.06%), Postives = 29/47 (61.70%), Query Frame = 3 
Query: 1404 KSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFN 1544
            K+   FK WLE+ KD   P   SYG  +GKLT YYD +S P P F+N
Sbjct:  234 KASKVFKEWLEQAKDKPRPTLNSYGYVNGKLTGYYDASSYPAPGFYN 280
[Close]
BLAST of SMED30010277 vs. Ensembl Mouse
Match: Ccdc34 (coiled-coil domain containing 34 [Source:MGI Symbol;Acc:MGI:1915451])

HSP 1 Score: 57.7658 bits (138), Expect = 1.967e-8
Identity = 25/53 (47.17%), Postives = 31/53 (58.49%), Query Frame = 3 
Query: 1407 SQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPV 1565
            ++  FK WLE  K+   P   SYG S GKLT +Y   S P P F+NP PW P+
Sbjct:  266 AEKKFKEWLENAKNKPRPAAKSYGYSSGKLTGFYSGNSYPEPTFYNPIPWKPI 318
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BLAST of SMED30010277 vs. UniProt/SwissProt
Match: sp|Q3UI66|CCD34_MOUSE (Coiled-coil domain-containing protein 34 OS=Mus musculus OX=10090 GN=Ccdc34 PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 1.376e-7
Identity = 25/53 (47.17%), Postives = 31/53 (58.49%), Query Frame = 3 
Query: 1407 SQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPV 1565
            ++  FK WLE  K+   P   SYG S GKLT +Y   S P P F+NP PW P+
Sbjct:  266 AEKKFKEWLENAKNKPRPAAKSYGYSSGKLTGFYSGNSYPEPTFYNPIPWKPI 318
[Close]
BLAST of SMED30010277 vs. UniProt/SwissProt
Match: sp|Q96HJ3|CCD34_HUMAN (Coiled-coil domain-containing protein 34 OS=Homo sapiens OX=9606 GN=CCDC34 PE=1 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 1.854e-7
Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 3 
Query: 1407 SQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPV 1565
            ++  F+ WLE  K    P   SYG ++GKLT +Y   S P PAF+NP PW P+
Sbjct:  272 AEKKFQEWLENAKHKPRPAAKSYGYANGKLTGFYSGNSYPEPAFYNPIPWKPI 324
[Close]
BLAST of SMED30010277 vs. TrEMBL
Match: A0A267GVH9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005156g2 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.977e-10
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 3 
Query: 1404 KSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPVIET 1574
            KS+ AF  W+E HK+     PYSYG +DG+L SYYDK SNPPP+F   +PW+P  ET
Sbjct:  359 KSEAAFAKWVEAHKNVRPHAPYSYGYTDGRLMSYYDKNSNPPPSFVVAEPWLPPTET 415
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BLAST of SMED30010277 vs. TrEMBL
Match: A0A267GXS2 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005156g1 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.097e-10
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 3 
Query: 1404 KSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPVIET 1574
            KS+ AF  W+E HK+     PYSYG +DG+L SYYDK SNPPP+F   +PW+P  ET
Sbjct:  362 KSEAAFAKWVEAHKNVRPHAPYSYGYTDGRLMSYYDKNSNPPPSFVVAEPWLPPTET 418
[Close]
BLAST of SMED30010277 vs. TrEMBL
Match: A0A267GBH3 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021986g1 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.154e-10
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 3 
Query: 1404 KSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPVIET 1574
            KS+ AF  W+E HK+     PYSYG +DG+L SYYDK SNPPP+F   +PW+P  ET
Sbjct:  362 KSEAAFAKWVEAHKNVRPHAPYSYGYTDGRLMSYYDKNSNPPPSFVVAEPWLPPTET 418
[Close]
BLAST of SMED30010277 vs. TrEMBL
Match: W5MHC9 (Coiled-coil domain containing 34 OS=Lepisosteus oculatus OX=7918 GN=CCDC34 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 7.278e-7
Identity = 27/54 (50.00%), Postives = 36/54 (66.67%), Query Frame = 3 
Query: 1404 KSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPV 1565
            K++  F+ WL+  KD   PV  S+  S+GKLT YYD TS P P+F+NP PW P+
Sbjct:  229 KAEEKFQEWLKSAKDKPRPVSNSFAYSNGKLTGYYDGTSYPAPSFYNPIPWKPI 282
[Close]
BLAST of SMED30010277 vs. TrEMBL
Match: W5MHB0 (Coiled-coil domain containing 34 OS=Lepisosteus oculatus OX=7918 GN=CCDC34 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 7.307e-7
Identity = 27/54 (50.00%), Postives = 36/54 (66.67%), Query Frame = 3 
Query: 1404 KSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPV 1565
            K++  F+ WL+  KD   PV  S+  S+GKLT YYD TS P P+F+NP PW P+
Sbjct:  235 KAEEKFQEWLKSAKDKPRPVSNSFAYSNGKLTGYYDGTSYPAPSFYNPIPWKPI 288
[Close]
BLAST of SMED30010277 vs. Planmine SMEST
Match: SMESG000000133.1 (SMESG000000133.1)

HSP 1 Score: 669.463 bits (1726), Expect = 0.000e+0
Identity = 490/491 (99.80%), Postives = 491/491 (100.00%), Query Frame = 3 
Query:  108 MSLRPEPVTSKFRKYYLNDILPIYHXXXXXXXXXXXXXXXXXXXXXXYRXXXXXXADLNYDSFDDLSIENNELKISDLPEQSLKRLNSQEFNSMTNKKFSKLKKQNGIFREIFNSSENRNFNVPKNRINKPSEKSCKRIIARKPDYPGSRITHVNYQQTMCEDRGLKPKKSSNPVHKNGIYKNKGNPMENEKASINEKSSSEKQIPRTIAKKSQNISENSIKDISTKAVSEQSDVPYSVCNFTSVSEEQKINQSNKSDKQSIDEXXXXXXXXXXXXXXXERSSVSNTDSVTDKQLPVPVPPQRMLNPWDDWFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCQKKIADWLAKKEEERIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMIKIAKSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPVIETKN 1580
            MSLRPEPVTSKFRKYYLNDILPIYHTSKLSLSNCSSTGSTSSLTSILYRNNNVNNADLNYDSFDDLSIENNELKISDLPEQSLKRLNSQEFNSMTNKKFSKLKKQNGIFREIFNSSENRNFNVPKNRINKPSEKSCKRIIARKPDYPGSRITHVNYQQTMCEDRGLKPKKSSNPVHKNGIYKNKGNPMENEKASINEKSSSEKQIPRTIAKKSQNISENSIKDISTKAVSEQSDVPYSVCNFTSVSEEQ+INQSNKSDKQSIDENNFSSIDDVNSISIDERSSVSNTDSVTDKQLPVPVPPQRMLNPWDDWFLRKEKEERKLSEELRKKMMREEAKKTEEAAAKEKRRELCQKKIADWLAKKEEERIKCLCKLKDEKKKKELKELEKDDLLKKSSKKYVSWKLKKIEQMKEKEKQEKEKQNKLEAEKMIKIAKSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPVIETKN
Sbjct:    1 MSLRPEPVTSKFRKYYLNDILPIYHTSKLSLSNCSSTGSTSSLTSILYRNNNVNNADLNYDSFDDLSIENNELKISDLPEQSLKRLNSQEFNSMTNKKFSKLKKQNGIFREIFNSSENRNFNVPKNRINKPSEKSCKRIIARKPDYPGSRITHVNYQQTMCEDRGLKPKKSSNPVHKNGIYKNKGNPMENEKASINEKSSSEKQIPRTIAKKSQNISENSIKDISTKAVSEQSDVPYSVCNFTSVSEEQQINQSNKSDKQSIDENNFSSIDDVNSISIDERSSVSNTDSVTDKQLPVPVPPQRMLNPWDDWFLRKEKEERKLSEELRKKMMREEAKKTEEAAAKEKRRELCQKKIADWLAKKEEERIKCLCKLKDEKKKKELKELEKDDLLKKSSKKYVSWKLKKIEQMKEKEKQEKEKQNKLEAEKMIKIAKSQTAFKHWLEEHKDHHLPVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPVIETKN 491
The following BLAST results are available for this feature:
BLAST of SMED30010277 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CCDC343.861e-845.28coiled-coil domain containing 34 [Source:HGNC Symb... [more]
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BLAST of SMED30010277 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010277 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010277 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010277 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cdc733.447e-751.06cell division cycle 73 [Source:Xenbase;Acc:XB-GENE... [more]
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BLAST of SMED30010277 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Ccdc341.967e-847.17coiled-coil domain containing 34 [Source:MGI Symbo... [more]
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BLAST of SMED30010277 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q3UI66|CCD34_MOUSE1.376e-747.17Coiled-coil domain-containing protein 34 OS=Mus mu... [more]
sp|Q96HJ3|CCD34_HUMAN1.854e-745.28Coiled-coil domain-containing protein 34 OS=Homo s... [more]
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BLAST of SMED30010277 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GVH91.977e-1056.14Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GXS22.097e-1056.14Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267GBH32.154e-1056.14Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
W5MHC97.278e-750.00Coiled-coil domain containing 34 OS=Lepisosteus oc... [more]
W5MHB07.307e-750.00Coiled-coil domain containing 34 OS=Lepisosteus oc... [more]
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BLAST of SMED30010277 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010277 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010277 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010277 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010277 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010277 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000000133.10.000e+099.80SMESG000000133.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010277 ID=SMED30010277|Name=SMED30010277|organism=Schmidtea mediterranea sexual|type=transcript|length=1951bp
ATTTAGAAGTCCGCCTTATTCAAAATATTTAAATTTAATGTTATTCGTTA
AATAAATCCTTACAAAACTGAATTATTTTGATATTTCAAAAACACAATTA
TTTAGTGATGTCTTTAAGGCCAGAACCTGTGACTTCTAAATTTAGGAAAT
ATTATCTTAATGATATTTTACCTATTTATCACACTAGTAAACTATCGCTA
TCCAATTGTTCATCTACCGGATCTACGTCGAGTTTAACATCGATTTTATA
TCGTAATAACAATGTCAATAATGCTGATTTGAACTACGATTCATTTGATG
ATTTATCAATCGAAAATAATGAATTGAAAATTTCTGATCTTCCTGAGCAG
TCTTTAAAACGATTAAATTCACAAGAATTTAATTCTATGACTAATAAAAA
GTTTTCCAAATTAAAAAAACAAAATGGTATTTTCCGCGAGATATTTAATT
CATCGGAAAATAGAAATTTCAACGTCCCGAAAAATAGAATCAACAAACCA
TCAGAGAAATCCTGTAAAAGAATAATTGCTCGAAAACCCGATTATCCGGG
ATCCAGAATAACTCATGTCAACTATCAGCAAACTATGTGTGAAGATAGAG
GTTTGAAGCCTAAAAAATCCTCTAACCCCGTTCACAAAAACGGCATTTAT
AAAAATAAAGGAAATCCAATGGAAAACGAAAAAGCTTCTATTAATGAAAA
ATCGAGCAGTGAAAAACAAATTCCAAGAACAATTGCAAAGAAAAGTCAAA
ACATCAGTGAAAATTCCATCAAAGATATCAGCACAAAAGCTGTGTCAGAG
CAATCCGATGTTCCATATTCAGTCTGTAATTTTACGTCGGTTTCTGAAGA
GCAGAAAATCAATCAGTCGAATAAATCCGACAAACAGAGCATTGATGAAA
ATAATTTCTCATCGATTGATGATGTGAATTCGATATCTATTGACGAGCGC
TCATCGGTTTCCAATACGGACTCCGTCACCGATAAGCAGCTGCCGGTGCC
GGTGCCGCCTCAGAGAATGCTCAATCCGTGGGATGATTGGTTTCTGAGGA
AAGAGAAGGAAGAGCGAAAGTTATCTGAGGAGCTACGTAAAAAAATGATG
AGAGAAGAGGCTAAGAAAACAGAAGAAGCAGCTGCTAAGGAAAAGCGTCG
AGAACTGTGTCAGAAGAAAATTGCTGATTGGTTGGCCAAAAAGGAGGAAG
AGCGCATAAAATGTCTATGCAAACTGAAAGATGAGAAAAAGAAAAAAGAG
CTTAAAGAGTTAGAAAAAGACGATTTATTGAAAAAGTCCAGTAAAAAATA
TGTTTCTTGGAAATTGAAAAAAATAGAACAAATGAAAGAAAAAGAAAAGC
AAGAAAAAGAAAAACAAAATAAACTGGAAGCTGAAAAAATGATAAAGATT
GCAAAATCACAAACTGCTTTCAAACATTGGTTAGAAGAACACAAAGACCA
CCATTTACCGGTTCCATACAGTTATGGAATATCGGATGGGAAACTTACAA
GCTATTACGATAAAACAAGCAATCCGCCGCCCGCATTTTTCAATCCGAAA
CCTTGGATCCCAGTGATTGAGACGAAAAATTGATCATTATCAATAGCGAT
TTATATTTATTAATTTATTGTAATTTATTGCACCAATGATTTACGGTTTC
ATTTGAAGTTTGAGAGCGTTTATCGGTGGGCATGACAAACTGTATTTAGA
CGGTGAAGTTTGCACTTGGTACGTTCCTTTATAAGGTCGATTAAATAACT
GACATCAGTAAGTGAATAATCAATAACGCGATTATTATTGATTATCAGAG
CAGTTGATTTAAATCACAGTCTGTGCATTTGACAGGAACATTATGAGGTT
GGTATTTTAGGTTCAAAACCAATAATTTCAAATCATTACTGTGATTATTG
ATTTTCAGAAGTTAATTTAAATCAAAAGTAACAAAATAAAATCGATTTAA
T
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protein sequence of SMED30010277-orf-1

>SMED30010277-orf-1 ID=SMED30010277-orf-1|Name=SMED30010277-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=492bp
MSLRPEPVTSKFRKYYLNDILPIYHTSKLSLSNCSSTGSTSSLTSILYRN
NNVNNADLNYDSFDDLSIENNELKISDLPEQSLKRLNSQEFNSMTNKKFS
KLKKQNGIFREIFNSSENRNFNVPKNRINKPSEKSCKRIIARKPDYPGSR
ITHVNYQQTMCEDRGLKPKKSSNPVHKNGIYKNKGNPMENEKASINEKSS
SEKQIPRTIAKKSQNISENSIKDISTKAVSEQSDVPYSVCNFTSVSEEQK
INQSNKSDKQSIDENNFSSIDDVNSISIDERSSVSNTDSVTDKQLPVPVP
PQRMLNPWDDWFLRKEKEERKLSEELRKKMMREEAKKTEEAAAKEKRREL
CQKKIADWLAKKEEERIKCLCKLKDEKKKKELKELEKDDLLKKSSKKYVS
WKLKKIEQMKEKEKQEKEKQNKLEAEKMIKIAKSQTAFKHWLEEHKDHHL
PVPYSYGISDGKLTSYYDKTSNPPPAFFNPKPWIPVIETKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR025259CCDC34
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 313..348
NoneNo IPR availableCOILSCoilCoilcoord: 360..393
NoneNo IPR availableCOILSCoilCoilcoord: 402..429
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..217
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..300
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 181..217
IPR025259Coiled-coil domain-containing protein 34PFAMPF13904DUF4207coord: 213..483
e-value: 3.1E-20
score: 72.9
IPR025259Coiled-coil domain-containing protein 34PANTHERPTHR23247NY-REN-41 ANTIGEN L15 -RELATEDcoord: 190..488