SMED30010266
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010266 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Alignments
SMED30010266 aligns in the following genomic locations:
Homology
BLAST of SMED30010266 vs. Planmine SMEST
Match: SMESG000064314.1 (SMESG000064314.1) HSP 1 Score: 273.092 bits (697), Expect = 2.016e-84 Identity = 174/181 (96.13%), Postives = 177/181 (97.79%), Query Frame = 1
Query: 85 ETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
ET++S DQIERE AESNI+E EEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIE
Sbjct: 2621 ETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIE 2801 HSP 2 Score: 182.57 bits (462), Expect = 7.836e-53 Identity = 107/158 (67.72%), Postives = 125/158 (79.11%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI---EGEITHKKINLDEVPKVDENNDSVD 465
GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNL+++SKV E D S D NESEPS S INE+ +GE ++VN ++VSK +E D+S DPN+S + YI EI + IT +NL+E+ KVDE + S D
Sbjct: 3254 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITD-PVNLNEISKVDEEDHSAD 3410 HSP 3 Score: 120.939 bits (302), Expect = 2.325e-31 Identity = 115/212 (54.25%), Postives = 147/212 (69.34%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGE-EEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITHK-KINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
GENN+AD V VDE S +E HSAD E EP+E NINE E E QE+FN +EV KV++ D+S DPN+SE S+I EI QGE NI+D VNVD+VSK +E+DH+ + N+SEP +S I E+ EGE + ++N +EV KV+E DS D NE EIVE NI+NV EG+T++SEE N+NE S IDE D SV N+S+L+EHYIIE
Sbjct: 3049 GENNIADSVNVDEVSKV----------DEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAEHYIIE 3250 HSP 4 Score: 117.087 bits (292), Expect = 4.640e-30 Identity = 89/151 (58.94%), Postives = 118/151 (78.15%), Query Frame = 1
Query: 175 FNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
N+ EVSKV+ETD + + NESEP SNI E+ GE ++VN+++VSK +E+D+S DPN SEP S INE+ G++TH+K NL+EV K EN+DSVDPNE EIV+ NI+ ++EG+TN+SEESN+NE S IDE D SV PN+S+L+EHYIIE
Sbjct: 2813 VNVDEVSKVEETDRTANLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIE 2963 HSP 5 Score: 103.219 bits (256), Expect = 2.855e-25 Identity = 101/242 (41.74%), Postives = 132/242 (54.55%), Query Frame = 1
Query: 4 ENNVADPVYVDEGSNFYGKDDSGDLNE-------------------------------ETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI---EGEITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
E N++DPV VDE S D + +LNE E D+S D EP +SNINE G + +T EKFNL+EVSK E D SVDPNE E NI + +GE NIS++ NV+++SK DE D SV PN+S+ + YI EI + IT +NL+E+ KVDE S D NE E E NI V+EG+ E+ N S ++E D S DPN+ EL++H I E
Sbjct: 2806 EKNISDPVNVDEVSKVEETDRTANLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGK-MTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITD-PVNLNEISKVDEKGHSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITE 3045 HSP 6 Score: 99.7525 bits (247), Expect = 4.892e-24 Identity = 77/159 (48.43%), Postives = 100/159 (62.89%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGE-EEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITHK-KINLDEVPKVDENNDSVDP 468
+NN+ DPV ++E S D+ G HSAD E EP+E NINE E E QE+ N + VSKV+E D+S DPNE E + NI EI QGE NI+D VNVD+VSK DE HS D +SEP + INE+ EGE + + N +EV KV++ ++S DP
Sbjct: 2967 SKNNITDPVNLNEISKV---DEKG-------HSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDP 3115 HSP 7 Score: 96.2857 bits (238), Expect = 8.052e-23 Identity = 88/209 (42.11%), Postives = 130/209 (62.20%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITHK-KINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYI 621
GE N+++ ++E SN + D S DLNE EP ESNIN+ EEE TQE+ +EVSKV E D SVDPNES+ NI ++ + EK ++N+D VSK DE+DHSVD N+S+P +S I E+ +GE T + ++N +EV VD+ SVD NE E+ NI +++G+ ++ +++ + + + D SVDPNK +L+E I
Sbjct: 1837 GERNISEQDNLEEVSNVFETDPSVDLNE----------NEPTESNINKVDEEETTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINLD-VSKLDETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESELANQNIIKIDKGE-----KNILDQVNVLYKTDHSVDPNKIKLAEFNI 2029 HSP 8 Score: 94.3597 bits (233), Expect = 3.697e-22 Identity = 95/204 (46.57%), Postives = 127/204 (62.25%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI---EGEITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSE 603
GE N++D V VD+ S KDD E+DHS D + EP +S INE E EIT +K NL EV KVDE + SVDPNE E SNI + +G+ N+S++ N+++ S DE D SVDPNKSE + YI EI E I+ +N+DEV KV+E + + + NE E ESNI+ V+ G+ E+ N+ E S ++E D S DPN SE
Sbjct: 2684 GEKNISDKVNVDQVS----KDD------ESDHSVDPNKSEPGKSYINEI-EGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQVEKNISD-PVNVDEVSKVEETDRTANLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSE 2875 HSP 9 Score: 92.0485 bits (227), Expect = 2.440e-21 Identity = 64/127 (50.39%), Postives = 90/127 (70.87%), Query Frame = 1
Query: 94 HSADQIEREPAESNINEAGEEEITQEK-FNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITHKKINLDEVPKVDENNDSVDP 468
+SAD E EPAESNINE + E Q+K +L+EVS VD+TDHS D NES+ + NI E+ GE+NIS++ N+++VS E+D SVD N++EP +S IN++ E E T +++ +EV KV E + SVDP
Sbjct: 1776 YSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDEEETTQEQLISEEVSKVGEKDVSVDP 1902 HSP 10 Score: 91.2781 bits (225), Expect = 4.635e-21 Identity = 84/182 (46.15%), Postives = 114/182 (62.64%), Query Frame = 1
Query: 85 ETDHSADQIEREPAESNINEAGEEEITQ-EKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITHK-KINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYI 621
ETDHS D E E AESNI+E E E Q E+ N +EVS VD TDHS +PNE E + NI + ++NIS+KVN+D+VSK DE D VDPN+SE +S IN++ EGE + ++N +EV V E +SV NE + ES + V+ + + +N E S +DE D S+ PN+SE +E I
Sbjct: 1445 ETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNI 1626 HSP 11 Score: 90.5077 bits (223), Expect = 8.077e-21 Identity = 104/253 (41.11%), Postives = 135/253 (53.36%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNE-------------------------------ETDHSADQIEREPAESNINEA--GEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVS----KDD------ESDHSVDPNKSEPGKSYINEI-EGEITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
GENN+AD V VDE S D +G+LNE E S D E E E NI GE +I++E N+ E+SK+DE D SV NES+ + I EI QGE N++D V VD+ S KDD E+DHS D + EP +S INE E EIT +K NL+++ KV E +DS D NE E ES I V+EG+ E+ N E S ++E D S DPN+S L+E+YI+E
Sbjct: 3131 GENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEP-NVNELSKIDEIDRSVYTNESKLAEHYIIEIDQGENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVE 3382 HSP 12 Score: 86.6557 bits (213), Expect = 1.844e-19 Identity = 69/172 (40.12%), Postives = 90/172 (52.33%), Query Frame = 1
Query: 85 ETDHSADQIEREPAESNINEAGEEEITQ------------------------------------------EKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKK--INLDEVPKVDENNDSVDP 468
ETDHS + EPAESNIN+ EEE Q E+ N+KE+ KV ET+HSVD NE E NI EI + EKNISD+VNVD++SK DE DHS DPN+SE S INE+ + T ++ +N E+ +V E ++S +P
Sbjct: 2335 ETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNP 2506 HSP 13 Score: 86.2705 bits (212), Expect = 2.410e-19 Identity = 80/180 (44.44%), Postives = 111/180 (61.67%), Query Frame = 1
Query: 91 DHSADQIEREPAESNINEAGEEEITQ-EKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITHK-KINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYI 621
D S + E E AESNI+E E E Q E+ N +EVS VD TDHS +PNE E + NI E+ ++NIS+KVN+D+VSK DE D VDPN+SE +S IN++ EGE + ++N +EV V E +SV NE + E + V+ + + +N E S +DE D S+ PN+SE +E I
Sbjct: 2173 DRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNI 2352 HSP 14 Score: 81.6481 bits (200), Expect = 7.800e-18 Identity = 60/138 (43.48%), Postives = 91/138 (65.94%), Query Frame = 1
Query: 64 DSGDLNEETDHSADQIEREPAESNINEAGEEEITQE-KFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITH--KKINLDEVPKVDENNDSVDP 468
D ++ +ETDHSA+ + EP+ES INE E + Q+ K +L VSKVD+ D S++ NESE + SNI+E+ + E ++VN ++VS D +DHS +PN+ E + I E++ + + +K+NLDEV KVDE + VDP
Sbjct: 2123 DQVNVVDETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDP 2260 HSP 15 Score: 79.337 bits (194), Expect = 4.555e-17 Identity = 100/249 (40.16%), Postives = 129/249 (51.81%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEA--GEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNV------------------------------------DQVSKDDESDHSVDPNKSEPGKSYINEI-EGEI-THKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
GE N+ D V V Y +TDHS D + + AE NINE GE + QE+ N EVSKVD D SVD NESE N+ EI + EKNIS+K NV DQV+ DE+DHS + NKSEP +S INE+ E + KK++LD V KVD+ + S++ NE E+ ESNI V+E +T E+ N E S +D D S +PN+ EL+E IIE
Sbjct: 1999 GEKNILDQVNV-----LY----------KTDHSVDPNKIKLAEFNINEVDNGEPK-QQEQVNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNVKELLNVNETDHSDDQNDGELAKQIIIEIDEVEKNILDQVNVVDETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIE 2231 HSP 16 Score: 76.6406 bits (187), Expect = 4.076e-16 Identity = 82/183 (44.81%), Postives = 113/183 (61.75%), Query Frame = 1
Query: 85 ETDHSADQIEREPAESNINEAGEEE--ITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEG--EITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYI 621
E +HS DQI+ E AE NI EEE IT + NLKE SKVD+ DHSV+ NE + EI + E+ +D V ++ SK DE +S DPN+SEP +S INE++ KKI+L+EV VD+ + S D NE ++ E NI V+ G+ N+SE+ N+ E S + E D SVD N++E +E I
Sbjct: 1691 EKEHSTDQIKSELAEPNIRNVDEEERNITADA-NLKEESKVDKIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNI 1872 HSP 17 Score: 74.7146 bits (182), Expect = 1.816e-15 Identity = 67/166 (40.36%), Postives = 87/166 (52.41%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNE-------------------------------ETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGE-KNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYI-NEIEGE 399
G+NN+ DPV ++E S +D S DLNE E D+S D E EPAESNINE G E +T EKFNL E SK+ E D S DPN+SE + NIN + + E K N ++ SK E D+S DPN++E + +I N +EGE
Sbjct: 3386 GKNNITDPVNLNEISKVDEEDHSADLNENEPSESNINEVDEGEPKQQEQVNPEGVSKVNEKDNSNDPSEIEPAESNINEFGGE-MTNEKFNLVEASKIFEKDDSFDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGE 3550 HSP 18 Score: 71.633 bits (174), Expect = 2.319e-14 Identity = 80/181 (44.20%), Postives = 114/181 (62.98%), Query Frame = 1
Query: 85 ETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHK--KINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYI 621
ETDHS DQ + E A+ I E E EK L +V+ VDETDHS + N+SEPS S INE+ + + KV++D VSK D+ D S++ N+SE +S I+E++ T + ++N +EV VD + S +PNE E+ E NI V+ K N+SE+ N++E S +DE D VDPN+SE +E I
Sbjct: 2094 ETDHSDDQNDGELAKQIIIEIDE----VEKNILDQVNVVDETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNI 2270 HSP 19 Score: 71.633 bits (174), Expect = 2.364e-14 Identity = 77/201 (38.31%), Postives = 119/201 (59.20%), Query Frame = 1
Query: 31 VDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNK---SEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYII 624
V+E N Y ++ D+S D E E+ IN+ EE I Q++ NL+EV KVDET+ ++PN SE + NI ++ + K +++N+++V+ DE D+SVD N+ +EP + +E I +++N +E K+DE + SVD NE E+ ESNI V+EG+T E+ N E S +D D S +PN+ EL+E II
Sbjct: 1310 VNETFNLYVMTKM----DQKDNSVDPYESAHIEAKINKIDEETIKQKQVNLEEVPKVDETEEFINPNRSELADHNIIKVEEETKK-QEQINLEEVTNVDERDYSVDQNEITLTEPIMKEVYNVEN-IQIQRVNSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNII 1504 HSP 20 Score: 67.781 bits (164), Expect = 4.555e-13 Identity = 48/128 (37.50%), Postives = 79/128 (61.72%), Query Frame = 1
Query: 88 TDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEI-THKKINLDEVPKVDENNDSVD 465
T+HS +Q++ + E NI + NL+++S+VDE DHSV+ NE +G N+ E+ +G+KN + N++++SK DE HS +PN+SE +S I E+ EGE K++N +E ++ N + VD
Sbjct: 3675 TEHSVNQMKCKIEERNITDDA---------NLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVNPNESTPIECNTNEVD 3793 HSP 21 Score: 66.2402 bits (160), Expect = 1.619e-12 Identity = 80/204 (39.22%), Postives = 119/204 (58.33%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKS---EPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSE 603
GE + V +E SN + ++S LNE T EP + ++ E I N +E SKVDETDHS+ PN+SEP+ SNIN++ + E ++VN D+V D D SV+P++S EP I E++ I+ + N+ E+ KV E N SVD NE E+ E NI +++ + N+S++ N++E S +DE D S DPN+SE
Sbjct: 2276 GETKQQEQVNFEEVSNVFELENSVILNENT-------LTEPIKKEVDNV--ENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEES-NVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESE 2469 HSP 22 Score: 65.0846 bits (157), Expect = 3.516e-12 Identity = 75/170 (44.12%), Postives = 107/170 (62.94%), Query Frame = 1
Query: 118 EPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITH-KKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYI 621
E A+ NI + EE QE+ NL+EV+ VDE D+SVD NE + + E+ E +VN ++ SK DE+DHSVD N+SE +S I+E+ EGE +++N +EV VD + S +PNE E+ E NI V+ K N+SE+ N++E S +DE D VDPN+SE +E I
Sbjct: 1375 ELADHNIIKVEEETKKQEQINLEEVTNVDERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNI 1544 HSP 23 Score: 65.0846 bits (157), Expect = 4.453e-12 Identity = 74/196 (37.76%), Postives = 113/196 (57.65%), Query Frame = 1
Query: 67 SGDLNEETDHSADQIEREPA------ESNINEAGEE-EITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDK-VNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHK-KINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
S D++E D ++E+ E+ IN+ GE + E FNL ++K+D+ D+SVDP ES + IN+I E+ I K VN+++V K DE++ ++PN+SE I ++E E + +INL+EV VDE + SVD NEI + E ++ V + + N E+S +DE D SVD N+SEL+E I E
Sbjct: 1271 SADISESNDKIHVRVEKHEKRADNLNETQINKLGENVDQVNETFNLYVMTKMDQKDNSVDPYESAHIEAKINKI--DEETIKQKQVNLEEVPKVDETEEFINPNRSELADHNIIKVEEETKKQEQINLEEVTNVDERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAESNISE 1464 HSP 24 Score: 63.5438 bits (153), Expect = 1.549e-11 Identity = 62/165 (37.58%), Postives = 82/165 (49.70%), Query Frame = 1
Query: 85 ETDHSADQIEREPAESNINEAGEEEITQ-EKFNLKEVSKVDET------------------------------------DHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKS---EPGKSYINEIEGEITHKKINLDEVPKVDENNDS 459
ETDHS D E +P ESNI E + E TQ E+ N +EVS VD+T DHSVDPN+ + + NINE+ GE ++VN D+VSK D D SVD N+S EP I+E+E I+ K N+ E+ V+E + S
Sbjct: 1935 ETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESELANQNIIKIDKGEKNILDQVNVLYKTDHSVDPNKIKLAEFNINEVDNGEPKQQEQVNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKS-NVKELLNVNETDHS 2098 HSP 25 Score: 63.1586 bits (152), Expect = 1.593e-11 Identity = 64/160 (40.00%), Postives = 87/160 (54.37%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKF-NLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYI---NEIEGEITHKKINLDEVPKVDENNDSVDP 468
E N++D V VDE S +E DHSAD E E A SNINE ++E Q++ N KE+S+V E D+S +PN+ E NI + + EKNI ++ + +S D + HSVD N+ E + I NE E + + NL EV KVDE S DP
Sbjct: 2440 SEKNISDQVNVDELSKV----------DEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNA-NLIEVSKVDEIYYSEDP 2588 HSP 26 Score: 56.9954 bits (136), Expect = 2.420e-9 Identity = 78/226 (34.51%), Postives = 113/226 (50.00%), Query Frame = 1
Query: 85 ETDHSADQIEREPAESNINEAGEEEITQ-------------EKFN-----------------------------LKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNK---SEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
ETDHS + EPAESNIN+ EEE Q EK+N +K +S VDE +HS D +SE + NI + + E+NI+ N+ + SK D+ DHSV+ N+ +EP I++ E T I+ +E K+DE S DPNE E ESNI V++ + ++ ++ E S +D+ D S D N+S+L+E IIE
Sbjct: 1609 ETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNVKSLSNVDEKEHSTDQIKSELAEPNIRNVDEEERNITADANLKEESKVDKIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVIS-EEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIE 1833 HSP 27 Score: 53.5286 bits (127), Expect = 3.292e-8 Identity = 57/160 (35.62%), Postives = 83/160 (51.88%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAES--NINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSE---PGKSYINEIEGEITHKKINLDEVPKVDENNDSVD 465
GE + V +E SN Y ++S LNE T + + E + E+ N++ EEE SKVDETDHS+ PN+SEP+ SNIN++ + E D+VN ++V + E +S DPN E P ++E E I + N+ + VDE S D
Sbjct: 1550 GETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEE-----------SKVDETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEES-NVKSLSNVDEKEHSTD 1697 HSP 28 Score: 53.1434 bits (126), Expect = 4.871e-8 Identity = 80/234 (34.19%), Postives = 119/234 (50.85%), Query Frame = 1
Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNE---------------------ETDHSADQIEREPAESNINEAGEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKI----NLDEVPKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELSEHYIIE 627
GE N+++ V E + +GK S D E ETDH+ D E EPAESN NE + +E+ N +E SKVDE D+S DP ++E I GEKNIS + + + K D+++HSV N+++ +I + I NL ++ +VDE + SV+ NE + N+ V++G+ N E +N+ E S +DE S +PN+SEL E I E
Sbjct: 3549 GEKNISEESNVKELTKVFGKQHSFDQIEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFNE-----VDEEEGNSEEESKVDEKDNSDDPYKNELVEPLIKNFNVGEKNISGESCLKDLLKVDKTEHSV------------NQMKCKIEERNITDDANLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYE 3765 HSP 29 Score: 46.595 bits (109), Expect = 7.316e-6 Identity = 46/133 (34.59%), Postives = 65/133 (48.87%), Query Frame = 1
Query: 85 ETDHSADQIEREPAESNINEAGEEEITQ-EKFNLKEVSKVDETDHSVDPNESE--------------------------PSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGE 399
E DHS + ER N+ E + + Q E NL+E+SKVDE HS +PNESE P N NE+ GEK ++ VN+++ S D+ D+ VDP +S K +N++ EGE
Sbjct: 3705 EIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVNPNESTPIECNTNEVDGGEKYQTEHVNLEEESMVDKIDNFVDPKESPFTKPDVNKVSEGE 3837 The following BLAST results are available for this feature:
BLAST of SMED30010266 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30010266 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30010266 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30010266 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30010266 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010266 ID=SMED30010266|Name=SMED30010266|organism=Schmidtea mediterranea sexual|type=transcript|length=629bpback to top protein sequence of SMED30010266-orf-1 >SMED30010266-orf-1 ID=SMED30010266-orf-1|Name=SMED30010266-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=209bp GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
