Hsp70-Hsp90 organizing 2

Overview
NameHsp70-Hsp90 organizing 2
Smed IDSMED30010240
Length (bp)1756
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Hsp70-Hsp90 organizing 2 (SMED30010240) t-SNE clustered cells

Violin plots show distribution of expression levels for Hsp70-Hsp90 organizing 2 (SMED30010240) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Hsp70-Hsp90 organizing 2 (SMED30010240) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Hsp70-Hsp90 organizing 2 (SMED30010240) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010240

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30010240h1SMcG0008037 Contig39808uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010240h1SMcG0008037 Contig39808newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30010240h1SMcG0008037 dd_Smed_v4_12016_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Human
Match: TOPORS (TOP1 binding arginine/serine rich protein [Source:HGNC Symbol;Acc:HGNC:21653])

HSP 1 Score: 51.9878 bits (123), Expect = 3.078e-6
Identity = 20/80 (25.00%), Postives = 42/80 (52.50%), Query Frame = 3 
Query:   81 SECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNK 320
            S+CP+C++    ++ S    C+H+FC  C+ +W    +   CPLCKQ    I +++R    + +      +N +++  ++
Sbjct:  101 SKCPICLDRF--DNVSYLDRCLHKFCFRCVQEWSKNKAE--CPLCKQPFDSIFHSVRAEDDFKEYVLRPSYNGSFVTPDR 176
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Human
Match: TOPORS (TOP1 binding arginine/serine rich protein [Source:HGNC Symbol;Acc:HGNC:21653])

HSP 1 Score: 51.6026 bits (122), Expect = 3.723e-6
Identity = 20/80 (25.00%), Postives = 42/80 (52.50%), Query Frame = 3 
Query:   81 SECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNK 320
            S+CP+C++    ++ S    C+H+FC  C+ +W    +   CPLCKQ    I +++R    + +      +N +++  ++
Sbjct:   36 SKCPICLDRF--DNVSYLDRCLHKFCFRCVQEWSKNKAE--CPLCKQPFDSIFHSVRAEDDFKEYVLRPSYNGSFVTPDR 111
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Fly
Match: Topors (gene:FBgn0267351 transcript:FBtr0331977)

HSP 1 Score: 53.5286 bits (127), Expect = 5.267e-7
Identity = 22/62 (35.48%), Postives = 32/62 (51.61%), Query Frame = 3 
Query:   87 CPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSK 272
            C +C+       +  T  CMH+FC +C+ +W        CPLCKQ    II+N+RT   Y +
Sbjct:  102 CAICLSR--CRRKCFTDSCMHQFCFKCLCEW--SKIKPECPLCKQPFRTIIHNVRTLDDYDR 159
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Fly
Match: Topors (gene:FBgn0267351 transcript:FBtr0086570)

HSP 1 Score: 53.5286 bits (127), Expect = 5.267e-7
Identity = 22/62 (35.48%), Postives = 32/62 (51.61%), Query Frame = 3 
Query:   87 CPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSK 272
            C +C+       +  T  CMH+FC +C+ +W        CPLCKQ    II+N+RT   Y +
Sbjct:  102 CAICLSR--CRRKCFTDSCMHQFCFKCLCEW--SKIKPECPLCKQPFRTIIHNVRTLDDYDR 159
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Zebrafish
Match: toporsb (topoisomerase I binding, arginine/serine-rich b [Source:ZFIN;Acc:ZDB-GENE-110728-3])

HSP 1 Score: 51.2174 bits (121), Expect = 3.628e-6
Identity = 19/67 (28.36%), Postives = 36/67 (53.73%), Query Frame = 3 
Query:   81 SECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITF 281
            S+CP+C++     + +    C+H+FC  CI +W    +   CPLCKQ  H I ++++  + + +   
Sbjct:   31 SKCPICLD--GFNNVASLDRCLHQFCFRCIHEWSKNKAE--CPLCKQPFHSIFHSVKGENDFKEFVL 93
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Mouse
Match: Topors (topoisomerase I binding, arginine/serine-rich [Source:MGI Symbol;Acc:MGI:2146189])

HSP 1 Score: 52.373 bits (124), Expect = 1.562e-6
Identity = 20/72 (27.78%), Postives = 37/72 (51.39%), Query Frame = 3 
Query:   81 SECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFN 296
            S+CP+C++    ++ S    C+H+FC  C+ +W    +   CPLCKQ    I +++R    + +      +N
Sbjct:  102 SKCPICLDRF--DNVSYLDRCLHKFCFRCVQEWSKNKAE--CPLCKQPFDSIFHSVRAEDDFKEYVLRPSYN 169
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. UniProt/SwissProt
Match: sp|P29129|ICP0_SUHVF (E3 ubiquitin-protein ligase ICP0 OS=Suid herpesvirus 1 (strain Indiana-Funkhauser / Becker) OX=31523 GN=EP0 PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 6.119e-7
Identity = 22/61 (36.07%), Postives = 42/61 (68.85%), Query Frame = 3 
Query:   84 ECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKY 266
            +CP+C++  + E++++  PCMH+FCL+CI +W    +ST CPLC   V  I++++ + + +
Sbjct:   45 DCPICLDVAATEAQTL--PCMHKFCLDCIQRWTL--TSTACPLCNARVTSILHHVDSDASF 101
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. UniProt/SwissProt
Match: sp|P84445|ICP0_EHV1V (E3 ubiquitin-protein ligase ICP0 OS=Equine herpesvirus 1 (strain V592) OX=310273 GN=ICP0 PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 3.696e-6
Identity = 45/177 (25.42%), Postives = 76/177 (42.94%), Query Frame = 3 
Query:   87 CPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKIKNILDNFENIIFKICSTVDSFLTNNKNDFDANI--LNYLVYFGNLSCRLKSSSTNTCLSTKDIPEDEECWPLVEQLVRKRLSLIFTNSFIATIFNNLI 611
            CP+C+E+ S  + S+  PC+H FC  CI +W+    +  CPLCK  V  +++ I + S++ +      F+         +   D+FE            FL  +  D  ANI   N  + F  L+    + +                 PLV+ LV +RL  +F    +A +  N++
Sbjct:    8 CPICLEDPS--NYSMALPCLHAFCYVCITRWI--RQNPTCPLCKVPVESVVHTIESDSEFKETKVSVDFDY------DSEEDEDSFEG----------QFLAVDSGDAPANISAWNGPMAFVPLNANGTAGAPR-------------LQPLVDWLV-ERLDQLFETPELALVMRNIV 150
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. UniProt/SwissProt
Match: sp|P28990|ICP0_EHV1B (E3 ubiquitin-protein ligase ICP0 OS=Equine herpesvirus 1 (strain Ab4p) OX=31520 GN=63 PE=1 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 3.696e-6
Identity = 45/177 (25.42%), Postives = 76/177 (42.94%), Query Frame = 3 
Query:   87 CPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKIKNILDNFENIIFKICSTVDSFLTNNKNDFDANI--LNYLVYFGNLSCRLKSSSTNTCLSTKDIPEDEECWPLVEQLVRKRLSLIFTNSFIATIFNNLI 611
            CP+C+E+ S  + S+  PC+H FC  CI +W+    +  CPLCK  V  +++ I + S++ +      F+         +   D+FE            FL  +  D  ANI   N  + F  L+    + +                 PLV+ LV +RL  +F    +A +  N++
Sbjct:    8 CPICLEDPS--NYSMALPCLHAFCYVCITRWI--RQNPTCPLCKVPVESVVHTIESDSEFKETKVSVDFDY------DSEEDEDSFEG----------QFLAVDSGDAPANISAWNGPMAFVPLNANGTAGAPR-------------LQPLVDWLV-ERLDQLFETPELALVMRNIV 150
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. UniProt/SwissProt
Match: sp|Q9V8P9|TOPRS_DROME (E3 ubiquitin-protein ligase Topors OS=Drosophila melanogaster OX=7227 GN=Topors PE=1 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 5.280e-6
Identity = 22/62 (35.48%), Postives = 32/62 (51.61%), Query Frame = 3 
Query:   87 CPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSK 272
            C +C+       +  T  CMH+FC +C+ +W        CPLCKQ    II+N+RT   Y +
Sbjct:  102 CAICLSR--CRRKCFTDSCMHQFCFKCLCEW--SKIKPECPLCKQPFRTIIHNVRTLDDYDR 159
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. TrEMBL
Match: T1FQT3 (RING-type domain-containing protein OS=Helobdella robusta OX=6412 GN=20211180 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 7.802e-10
Identity = 29/78 (37.18%), Postives = 48/78 (61.54%), Query Frame = 3 
Query:   33 MKRKNDDKKSIFKYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKY 266
            ++   D++++       EC +C+ ++  ++RSV S C HEFC  CIGKW     + +CPLCKQ+   I++NIR+ + Y
Sbjct:   12 VRMPGDERETSSSTDAIECSICLSDIEPQARSVISICFHEFCFSCIGKW--SKENAVCPLCKQNFTEILHNIRSDADY 87
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. TrEMBL
Match: T1FZ36 (RING-type domain-containing protein OS=Helobdella robusta OX=6412 GN=20214084 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.363e-9
Identity = 30/78 (38.46%), Postives = 45/78 (57.69%), Query Frame = 3 
Query:   33 MKRKNDDKKSIFKYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKY 266
            +K    +KKS      ++C +C+  +  + RSV S C+HEFC  CI KW     + +CPLCKQ+   I+ NIR+ + Y
Sbjct:    1 VKMPTSNKKSSPNSAANQCAICLSNIEPKKRSVISTCLHEFCFSCIKKW--SKENAVCPLCKQNFTEILSNIRSDADY 76
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. TrEMBL
Match: T1FQT5 (RING-type domain-containing protein OS=Helobdella robusta OX=6412 GN=20211182 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 8.740e-8
Identity = 26/61 (42.62%), Postives = 42/61 (68.85%), Query Frame = 3 
Query:   84 ECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKY 266
            EC +C+ ++  +++SV S C+HEFC  CI KW  G++  +CPLC Q+   I++NIR+ + Y
Sbjct:   12 ECSICLSDIKPQTKSVISKCLHEFCFSCIRKWSEGNA--ICPLCLQNFTEILHNIRSDNDY 70
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. TrEMBL
Match: T1EU19 (RING-type domain-containing protein OS=Helobdella robusta OX=6412 GN=20200069 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.555e-7
Identity = 30/71 (42.25%), Postives = 44/71 (61.97%), Query Frame = 3 
Query:   78 GSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYS--KITFP 284
             S C +C+  M+ E+RSV   C+HEFC  CIGKW     + +CPLC+Q+ + ++ NIR+   Y   +IT P
Sbjct:    8 ASLCTICLSVMNPENRSVLLTCLHEFCFPCIGKW--AEKNAVCPLCRQNSNEVLNNIRSDDDYDIYQITRP 76
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. TrEMBL
Match: T1EU20 (RING-type domain-containing protein OS=Helobdella robusta OX=6412 GN=20200070 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.815e-7
Identity = 31/76 (40.79%), Postives = 44/76 (57.89%), Query Frame = 3 
Query:   78 GSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYS--KITFPKGFNC 299
             S C +C+  M  E+RSV   C+HEFC  CIGKW     + +CPLC+Q+ + ++ NIR+   Y   +IT P   N 
Sbjct:    8 ASLCTICLSVMKPENRSVLLTCLHEFCFPCIGKW--AEKNAVCPLCRQNFNEVLNNIRSDDDYDIYQITRPISNNA 81
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Cavefish
Match: toporsa (topoisomerase I binding, arginine/serine-rich a [Source:ZFIN;Acc:ZDB-GENE-030131-6401])

HSP 1 Score: 50.447 bits (119), Expect = 4.834e-6
Identity = 20/64 (31.25%), Postives = 34/64 (53.12%), Query Frame = 3 
Query:   81 SECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSK 272
            S+CP+C++     + S    C+H FC  CI +W    +   CPLCKQ    I ++I++   + +
Sbjct:   70 SKCPICLD--GFRNISYVDKCLHRFCFRCIQEWAKNKAE--CPLCKQPFTSIYHSIQSEHDFQQ 129
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Cavefish
Match: rnf6 (ring finger protein 6 [Source:NCBI gene;Acc:103037065])

HSP 1 Score: 49.6766 bits (117), Expect = 7.323e-6
Identity = 22/58 (37.93%), Postives = 34/58 (58.62%), Query Frame = 3 
Query:   54 KKSIFKYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSV 227
            + S+   QG  C VCI E +  ++  + PC HEF + CI +WL  S +  CP+C+Q +
Sbjct:  631 QASLEGEQGRACSVCINEYAQGNKLRSLPCAHEFHIHCIDRWL--SENNTCPICRQPI 686
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Cavefish
Match: toporsb (E3 ubiquitin-protein ligase Topors-like [Source:NCBI gene;Acc:103043679])

HSP 1 Score: 49.6766 bits (117), Expect = 9.887e-6
Identity = 18/64 (28.12%), Postives = 35/64 (54.69%), Query Frame = 3 
Query:   81 SECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSK 272
            S+CP+C++  +  + +    C+H FC  CI +W    +   CPLCKQ    I ++++  + + +
Sbjct:   31 SKCPICLDRFN--NAASLDRCLHRFCFRCIHEWSKNKAE--CPLCKQPFRSIFHSVKAENDFKE 90
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Cavefish
Match: toporsb (E3 ubiquitin-protein ligase Topors-like [Source:NCBI gene;Acc:103043679])

HSP 1 Score: 49.6766 bits (117), Expect = 9.887e-6
Identity = 18/64 (28.12%), Postives = 35/64 (54.69%), Query Frame = 3 
Query:   81 SECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSK 272
            S+CP+C++  +  + +    C+H FC  CI +W    +   CPLCKQ    I ++++  + + +
Sbjct:   31 SKCPICLDRFN--NAASLDRCLHRFCFRCIHEWSKNKAE--CPLCKQPFRSIFHSVKAENDFKE 90
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Cavefish
Match: toporsb (E3 ubiquitin-protein ligase Topors-like [Source:NCBI gene;Acc:103043679])

HSP 1 Score: 49.6766 bits (117), Expect = 9.887e-6
Identity = 18/64 (28.12%), Postives = 35/64 (54.69%), Query Frame = 3 
Query:   81 SECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSK 272
            S+CP+C++  +  + +    C+H FC  CI +W    +   CPLCKQ    I ++++  + + +
Sbjct:   31 SKCPICLDRFN--NAASLDRCLHRFCFRCIHEWSKNKAE--CPLCKQPFRSIFHSVKAENDFKE 90
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Planmine SMEST
Match: SMESG000024446.1 (SMESG000024446.1)

HSP 1 Score: 1025.39 bits (2650), Expect = 0.000e+0
Identity = 540/542 (99.63%), Postives = 540/542 (99.63%), Query Frame = 3 
Query:   33 MKRKNDDKKSIFKYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKIKNILDNFENIIFKICSTVDSFLTNNKNDFDANILNYLVYFGNLSCRLKSSSTNTCLSTKDIPEDEECWPLVEQLVRKRLSLIFTNSFIATIFNNLIGKSISYKKLLTILKRHLDPVRFNCLKSDIVISLQMLRDPSLYLEYYVPGDHEHVIYTKSELVDKSTTAAVITRVITSINIENLDFTIAGYQITNIDYIFCILDYFCSFQNLITHLGFSSYYILLSKKFYSSLDLTKPATTMTTINASTCHLIKHCFKDEDLDHINQTLKNFETRLIYFEPLLVYDSSNSIQSXXXXXXXXXXXXXXXXXSCPQSDIVIELSDSDEDELQIISVNQPVFDLTESFSDDNDVILEEIPNKKRKISFSSEEEIDDKIDLQKILGLCSSDFQPTTSFNAEHQENVPQAFDXXXXXXXXXXXXXECLSDDDEVDVRDINFIYQPRSDNDFYNYL 1658
            MKRKNDDKKSIFKYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKIKNILDNFENIIFKICSTVDSFLTNNKNDFDANILNYLVYFGNLSCRLKSSSTNTCLSTKDIPEDEECWPLVEQLVRKRLSLIFTNSFIATIFNNLIGKSISYKKLLTILKRHLDPVRFNCLKSDIVISLQMLRDPSLYLEYYVPGDHEHVIYTKSELVDKST AAVITRVITSINIENLDFTIAGYQITNIDYIFCILDYFCSFQNLITHLGFSSYYILLSKKFYSSLDLTKPATTMTTINASTCHLIKHCFKDEDLDHINQTL NFETRLIYFEPLLVYDSSNSIQSEYDEQYEGEEEEDEDYYSCPQSDIVIELSDSDEDELQIISVNQPVFDLTESFSDDNDVILEEIPNKKRKISFSSEEEIDDKIDLQKILGLCSSDFQPTTSFNAEHQENVPQAFDSVSPSVASAVRSVECLSDDDEVDVRDINFIYQPRSDNDFYNYL
Sbjct:    1 MKRKNDDKKSIFKYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKIKNILDNFENIIFKICSTVDSFLTNNKNDFDANILNYLVYFGNLSCRLKSSSTNTCLSTKDIPEDEECWPLVEQLVRKRLSLIFTNSFIATIFNNLIGKSISYKKLLTILKRHLDPVRFNCLKSDIVISLQMLRDPSLYLEYYVPGDHEHVIYTKSELVDKSTAAAVITRVITSINIENLDFTIAGYQITNIDYIFCILDYFCSFQNLITHLGFSSYYILLSKKFYSSLDLTKPATTMTTINASTCHLIKHCFKDEDLDHINQTLTNFETRLIYFEPLLVYDSSNSIQSEYDEQYEGEEEEDEDYYSCPQSDIVIELSDSDEDELQIISVNQPVFDLTESFSDDNDVILEEIPNKKRKISFSSEEEIDDKIDLQKILGLCSSDFQPTTSFNAEHQENVPQAFDSVSPSVASAVRSVECLSDDDEVDVRDINFIYQPRSDNDFYNYL 542
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Planmine SMEST
Match: SMESG000081165.1 (SMESG000081165.1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.489e-11
Identity = 34/85 (40.00%), Postives = 48/85 (56.47%), Query Frame = 3 
Query:   69 KYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKI 323
            K++   CP+C++  +I++    +PC+HEFCL CIG WL    S  CP CKQ  + IIY +     +S IT    F  AW+ R  I
Sbjct:   13 KFKEDICPICMD--TIKNLIALNPCLHEFCLNCIGMWLVKHFS--CPKCKQIPYSIIYKVTNDFFHSFITLNVTFVDAWMQRGLI 93
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Planmine SMEST
Match: SMESG000081165.1 (SMESG000081165.1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.932e-11
Identity = 34/85 (40.00%), Postives = 48/85 (56.47%), Query Frame = 3 
Query:   69 KYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKI 323
            K++   CP+C++  +I++    +PC+HEFCL CIG WL    S  CP CKQ  + IIY +     +S IT    F  AW+ R  I
Sbjct:   13 KFKEDICPICMD--TIKNLIALNPCLHEFCLNCIGMWLVKHFS--CPKCKQIPYSIIYKVTNDFFHSFITLNVTFVDAWMQRGLI 93
[Close]
BLAST of Hsp70-Hsp90 organizing 2 vs. Planmine SMEST
Match: SMESG000062719.1 (SMESG000062719.1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.116e-11
Identity = 44/128 (34.38%), Postives = 59/128 (46.09%), Query Frame = 3 
Query:   69 KYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKIKNILDNFENIIFKICSTVDSFLTNNKNDFDANILNYLVYFGNL 452
            K+    C +C++  +I        C+HEFCL+CI  WL  + S  CP CKQ  H ++Y I+  S  S I   K F   WL  N I +   N    I K+   + SFL N K           VYF N+
Sbjct:   26 KFIEETCAICMD--NIRHCIALDTCLHEFCLDCICNWLLKAVS--CPQCKQIPHVLLYKIQNDSFTSGIRLDKKFEEEWLKHNLIVDFFSNRTYYIKKLKKLLISFLKNFK--LTIQCQRIFVYFCNM 147
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BLAST of Hsp70-Hsp90 organizing 2 vs. Planmine SMEST
Match: SMESG000062719.1 (SMESG000062719.1)

HSP 1 Score: 64.6994 bits (156), Expect = 8.094e-11
Identity = 61/211 (28.91%), Postives = 89/211 (42.18%), Query Frame = 3 
Query:   69 KYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWLTGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKIKNILDNFENIIFKICSTVDSFLTNNKNDFDANILNYLVYFGNLSCRLKSSSTNTCLSTKDIPEDEECWPL-----VEQLVRKRLSLIFTNSFIATIFNNLIGKSISYKKLLTILKRHLDPVRFNCL 686
            K+    C +C++  +I        C+HEFCL+CI  WL  + S  CP CKQ  H ++Y I+  S  S I   K F   WL  N I +   N    I K+   + SFL N K       +   VYF N+          T      IP + +   +     +   +   LS+ F +S +    +N   K  +Y+ L+  L   L P RF  L
Sbjct:   26 KFIEETCAICMD--NIRHCIALDTCLHEFCLDCICNWLLKAVS--CPQCKQIPHVLLYKIQNDSFTSGIRLDKKFEEEWLKHNLIVDFFSNRTYYIKKLKKLLISFLKNFKLTIQCQRI--FVYFCNMK--------TTLFKRYQIPWNPDNLTIDPSHKIWHWLSIELSVFFESSTVKKSISNFQIKVFNYRDLVKELSALL-PDRFRSL 221
The following BLAST results are available for this feature:
BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TOPORS3.078e-625.00TOP1 binding arginine/serine rich protein [Source:... [more]
TOPORS3.723e-625.00TOP1 binding arginine/serine rich protein [Source:... [more]
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Topors5.267e-735.48gene:FBgn0267351 transcript:FBtr0331977[more]
Topors5.267e-735.48gene:FBgn0267351 transcript:FBtr0086570[more]
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
toporsb3.628e-628.36topoisomerase I binding, arginine/serine-rich b [S... [more]
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Topors1.562e-627.78topoisomerase I binding, arginine/serine-rich [Sou... [more]
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BLAST of Hsp70-Hsp90 organizing 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|P29129|ICP0_SUHVF6.119e-736.07E3 ubiquitin-protein ligase ICP0 OS=Suid herpesvir... [more]
sp|P84445|ICP0_EHV1V3.696e-625.42E3 ubiquitin-protein ligase ICP0 OS=Equine herpesv... [more]
sp|P28990|ICP0_EHV1B3.696e-625.42E3 ubiquitin-protein ligase ICP0 OS=Equine herpesv... [more]
sp|Q9V8P9|TOPRS_DROME5.280e-635.48E3 ubiquitin-protein ligase Topors OS=Drosophila m... [more]
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BLAST of Hsp70-Hsp90 organizing 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1FQT37.802e-1037.18RING-type domain-containing protein OS=Helobdella ... [more]
T1FZ364.363e-938.46RING-type domain-containing protein OS=Helobdella ... [more]
T1FQT58.740e-842.62RING-type domain-containing protein OS=Helobdella ... [more]
T1EU191.555e-742.25RING-type domain-containing protein OS=Helobdella ... [more]
T1EU203.815e-740.79RING-type domain-containing protein OS=Helobdella ... [more]
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
toporsa4.834e-631.25topoisomerase I binding, arginine/serine-rich a [S... [more]
rnf67.323e-637.93ring finger protein 6 [Source:NCBI gene;Acc:103037... [more]
toporsb9.887e-628.13E3 ubiquitin-protein ligase Topors-like [Source:NC... [more]
toporsb9.887e-628.13E3 ubiquitin-protein ligase Topors-like [Source:NC... [more]
toporsb9.887e-628.13E3 ubiquitin-protein ligase Topors-like [Source:NC... [more]
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Hsp70-Hsp90 organizing 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Hsp70-Hsp90 organizing 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000024446.10.000e+099.63SMESG000024446.1[more]
SMESG000081165.11.489e-1140.00SMESG000081165.1[more]
SMESG000081165.13.932e-1140.00SMESG000081165.1[more]
SMESG000062719.14.116e-1134.38SMESG000062719.1[more]
SMESG000062719.18.094e-1128.91SMESG000062719.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010240 ID=SMED30010240|Name=Hsp70-Hsp90 organizing 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1756bp
TTTGATTTAATAACTAAGTTTATTTCAAGTTAATGAAGAGGAAAAATGAC
GATAAGAAATCGATTTTTAAATACCAAGGTTCTGAATGCCCTGTTTGTAT
TGAAGAAATGAGTATTGAGAGTCGTTCAGTTACTTCTCCTTGTATGCATG
AATTTTGCTTAGAATGTATTGGAAAGTGGCTTACTGGATCCTCCAGTACG
CTATGTCCTTTGTGTAAGCAAAGTGTTCATCACATTATATACAACATTCG
AACGCCTTCAAAATATTCCAAAATAACCTTTCCAAAAGGATTTAACTGTG
CTTGGCTTTTGAGAAACAAAATAAAAAATATTTTAGATAACTTTGAAAAC
ATAATTTTTAAAATATGTTCTACTGTTGATTCTTTTCTGACCAACAACAA
GAATGATTTCGATGCCAATATTTTAAATTATTTAGTTTATTTTGGAAATT
TATCATGCCGACTAAAGTCAAGTTCAACCAACACATGTCTATCAACGAAA
GATATTCCAGAAGATGAAGAGTGTTGGCCTTTAGTAGAGCAATTAGTTCG
TAAACGGTTATCTTTAATATTTACCAACAGCTTCATTGCCACGATCTTCA
ATAATCTTATTGGCAAATCTATTTCCTATAAGAAATTGTTGACGATTTTG
AAACGTCATTTAGACCCAGTGCGATTCAATTGTCTAAAATCGGACATTGT
GATTTCCTTACAAATGCTGCGTGATCCCTCATTATATCTGGAATATTATG
TCCCAGGAGATCACGAGCATGTCATCTACACAAAGTCTGAATTGGTGGAC
AAAAGCACCACCGCTGCAGTTATCACACGAGTAATCACCTCTATCAACAT
TGAAAACTTAGATTTTACTATTGCTGGCTATCAAATCACCAATATCGATT
ACATCTTTTGCATTCTGGACTATTTCTGTTCCTTTCAAAATCTTATTACC
CATTTGGGTTTCTCGTCGTATTATATTCTACTCAGCAAGAAATTCTATTC
GTCTCTCGATCTTACAAAACCCGCGACTACCATGACAACAATCAACGCAT
CAACTTGTCATCTTATCAAACACTGTTTCAAAGATGAAGATTTGGATCAC
ATCAATCAGACTCTGAAAAATTTCGAAACACGATTAATATATTTCGAGCC
GTTACTTGTTTATGATTCCTCAAATTCCATACAATCGGAATACGACGAAC
AATACGAAGGAGAAGAAGAAGAGGATGAAGATTATTACAGTTGTCCACAG
TCTGATATCGTTATCGAACTCAGCGATTCTGATGAGGATGAGTTGCAAAT
CATTTCTGTGAATCAGCCGGTCTTTGATCTAACCGAATCCTTTTCCGATG
ATAATGATGTGATATTGGAGGAAATTCCGAATAAAAAACGGAAAATATCT
TTTTCCAGTGAAGAAGAAATCGATGATAAAATCGATTTACAGAAAATATT
AGGTCTCTGTTCTTCAGATTTCCAACCGACAACTTCTTTCAATGCAGAAC
ATCAGGAAAATGTGCCACAAGCTTTCGATTCAGTCAGTCCTTCTGTAGCT
TCAGCAGTCCGAAGCGTCGAGTGCCTGTCAGACGATGATGAAGTCGATGT
TCGAGACATCAATTTCATTTATCAGCCCAGATCAGACAATGATTTCTACA
ACTATTTAATGCCACCAAATCCCCCTGACAGCTAGAATCCATGTGATTGT
ATGTAAATATATAATTTTCGTTCTCTCTCCCATTATTGTACTAGAAATAA
TTTAAG
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protein sequence of SMED30010240-orf-1

>SMED30010240-orf-1 ID=SMED30010240-orf-1|Name=SMED30010240-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=551bp
MKRKNDDKKSIFKYQGSECPVCIEEMSIESRSVTSPCMHEFCLECIGKWL
TGSSSTLCPLCKQSVHHIIYNIRTPSKYSKITFPKGFNCAWLLRNKIKNI
LDNFENIIFKICSTVDSFLTNNKNDFDANILNYLVYFGNLSCRLKSSSTN
TCLSTKDIPEDEECWPLVEQLVRKRLSLIFTNSFIATIFNNLIGKSISYK
KLLTILKRHLDPVRFNCLKSDIVISLQMLRDPSLYLEYYVPGDHEHVIYT
KSELVDKSTTAAVITRVITSINIENLDFTIAGYQITNIDYIFCILDYFCS
FQNLITHLGFSSYYILLSKKFYSSLDLTKPATTMTTINASTCHLIKHCFK
DEDLDHINQTLKNFETRLIYFEPLLVYDSSNSIQSEYDEQYEGEEEEDED
YYSCPQSDIVIELSDSDEDELQIISVNQPVFDLTESFSDDNDVILEEIPN
KKRKISFSSEEEIDDKIDLQKILGLCSSDFQPTTSFNAEHQENVPQAFDS
VSPSVASAVRSVECLSDDDEVDVRDINFIYQPRSDNDFYNYLMPPNPPDS
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
IPR017907Znf_RING_CS
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
GO:0016874ligase activity
GO:0046872metal ion binding