DDE_3 domain-containing protein

Overview
NameDDE_3 domain-containing protein
Smed IDSMED30010233
Length (bp)1253
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DDE_3 domain-containing protein (SMED30010233) t-SNE clustered cells

Violin plots show distribution of expression levels for DDE_3 domain-containing protein (SMED30010233) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DDE_3 domain-containing protein (SMED30010233) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DDE_3 domain-containing protein (SMED30010233) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010233

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30010233SMESG000063405.1 SmedASXL_007569SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30010233SMESG000063405.1 SmedASXL_007569SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30010233SMESG000063405.1 dd_Smed_v4_13863_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DDE_3 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000000571.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:1:80446753:80447784:1 gene:ENSXETG00000005985.1 transcript:ENSXETT00000000571.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.9954 bits (136), Expect = 2.288e-8
Identity = 68/302 (22.52%), Postives = 132/302 (43.71%), Query Frame = 3 
Query:  231 PVRKTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTRGKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQQNIHISIYSISRFLKKNFV--TFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDE-----------CYFNTWARKTTGRSENGERIFVK---VPTTV--GPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINE------VTTKLNETGFILVHDN-----VRIYDENISPNN-QMVKLPPYSSFLNPIEEVFIKIKENL 1046
            P  K I ++ + K++E  + G+ + A+ K +G+ ++T+ SII+ + + G V       + TKI+    ++L+    K+  +T +E+   L+   + +   +I + L KN +   F + +  +  KN  + L   K ++    +  W N  TL+ DE           CY   W +  T         F K   +PT    G ++ +      +GP    ++ G++    YQ  + E         KL  + ++L  DN      +   E +  N  + ++ P  S  LNPIE ++  +K+ +
Sbjct:    2 PRSKEIEEQMRTKVIEIYQSGKGYKAISKALGLQRTTVRSIIHKWQKHGTVVNLPRSGRPTKITPRAQRQLIREATKDPRTTSKELQASLASIKVSVHDSTIRKRLGKNGLHGRFPRRKPLLSKKNIMARLNFAKKHLND-CQDFWEN--TLWTDETKVELFGRCVSCYI--WRKSNTA--------FQKKNIIPTVKYGGGSVMVWGCFAASGPGRLAVIDGTMNSTVYQKILKENVRPSVRQLKLKRS-WVLQQDNDPKHTSKSTSEWLKKNKMKTLEWPSQSPDLNPIEMLWHDLKKAV 289
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A0C2JI12 (DDE_3 domain-containing protein OS=Thelohanellus kitauei OX=669202 GN=RF11_01522 PE=4 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 1.939e-32
Identity = 95/337 (28.19%), Postives = 172/337 (51.04%), Query Frame = 3 
Query:  246 ISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTRGKPG-TKNTKISEEMPQKLLNFIDKNYCSTLEEM-SKYLSQQNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLE--QGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTK---LNETGFILVHDNVRIYD-ENIS-----PNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNIQGFVSMNVNNRFIILNDAINKELKKEDYSDLKSYYKHIRKFFPKSLLKEV 1217
            +S  D+ ++V+A +KG+    +   +G+A+STINS+I +++Q+G++   K G  +  K++E+  +++ +++D N    L  + +K   + +I +   +I R L++   ++++V      +N++S L +R+ Y  R LE  Q +   + ++IDEC  N   R + GRS+ G      VP     NI +  AM VNG + HK    +  ++ +  +I+E+  K   LN     +V DNV  +  E IS       + ++ LPPYS F+NPIE +F K K  +R   S     +  N+     ++             SD   YY H+ ++  + L +EV
Sbjct:    6 LSDLDRKRIVDAYQKGQKASEISLVLGVARSTINSVIKIFHQSGRIDSNKRGYIQPEKLNEDQKERIKSWVDDNAGIPLRTIVTKVQEEMDISVGKSTIDRILQRFHYSWKRVSIIPERRNNESTLNIRERYATRYLELLQSFREHQFIFIDECGVNISMRTSHGRSKIGSPAIHVVPNLRSRNISICAAMNVNGMLLHKWHDRAFNRDLFGNYIDELLEKIRALNIEHATIVMDNVSFHHCEEISQQISEAGHTLLFLPPYSPFMNPIENMFSKWKREIRYRRSENSEELYQNITAASSLITS-----------SDCSGYYSHMFRYINRCLRREV 331
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A267DQJ8 (DDE_3 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002000g2 PE=4 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 1.147e-31
Identity = 81/292 (27.74%), Postives = 153/292 (52.40%), Query Frame = 3 
Query:  216 PSKEKPVRKTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKV-TRGKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQ---QNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVPTTVGPNICLVLAMG-VNGPIHHKLLIGSLAKESYQTFINEVTTKLNETGFILVHDNVRIYDE--NISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNIQ 1070
            P+ ++  R  +SQ+D+ +LV A    E++  L +Q+ I + +  ++I  + +TG+V    + G K   +S+EM + L+ F+     +TL++M ++L Q        +I +ISR L    +T + +R+     N+D +   R+ + + L+ +G   K  ++ DE   N W  +T GR+  G R    V    G N+ + LA+  + G ++ K++ G + +E +  F  E+   ++E  F L+HDN R + +   +    +   LPPYS FLN  E     +K +L++S+S ++
Sbjct:    4 PAAQR--RVAVSQQDRRRLVNAFVLNEDWLNLAEQLNIPRQSARNVIVKFERTGRVEAEKRGGNKPKALSDEMIEALVGFVADTPAATLQDMRRHLMQAFPDWQACTIQTISRALDGQLITVKLLRTIPNAWNADRIKHERREFAKWLMTEG-MGKLLVFCDEMGCNVWTARTQGRAAEGVRAVRIVANQRGRNVTMCLAVSPMRGLVYWKIVRGGMTRELFAEFFAELAALVDEP-FCLLHDNARAHWDPPAMGEEQEYRPLPPYSPFLNMTERAIAAVKASLKRSLSRVE 291
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: W4XP44 (DDE_3 domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 4.795e-30
Identity = 92/352 (26.14%), Postives = 164/352 (46.59%), Query Frame = 3 
Query:  246 ISQRDKMKLVEAME-KGENFHALGKQMGIAKSTINSIIYLYNQTGKVTR-GKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQ---QNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENG--ERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTKLNE-----TGFILVHDNVRIY-----DENISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMS-----------NIQGFVSMNVNN-RFIILNDAINKELKKEDYSDLKSYYKHIRKFFPKSLLKE 1214
            IS  D+ +LVEA E  G+++  L   +GI +ST  SI+  Y  +G++ +  + G +N+KI EEM   L + ID N   TL +M   L +   Q   +S  +I+R L    +T +        +N+  VL+ R+ Y    LE G      ++IDEC +N W R+T GR+  G   R  V        N+   ++  V G +HH++   ++ + S++ F+     + NE        +L++DN R +      E+++   +  +LPPYS FLN  E      K  ++++++              G   +N+   R   L +   + +     +    +Y H + + P+ L ++
Sbjct:   10 ISNVDRRRLVEAFEGNGQDYLELADNLGIKRSTARSIVATYLDSGRIEKLPRGGARNSKIDEEMKNYLQDIIDTNPLLTLRQMKDKLREDLPQKPEVSTTTIARALDAMLMTLKLAEDVPDARNAPRVLQQREEYAEWFLEHG-VLANCVFIDECGYNIWTRRTQGRAPRGVPARRVVHGQRGRNCNVSFAVSAAV-GLVHHEMAFETVTRASFERFLAATVAECNEMFPDDAPVVLIYDNARPHVRAELPEDVNSLIETKRLPPYSPFLNMTEMAHSSFKSAVKRTLALPAWQRRVGDRVAAGRAGVNLQQWRCDALREVAVQCIDTVTPAKCTQWYNHSQTYMPRCLARQ 359
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: W4Z6F7 (DDE_3 domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 7.295e-29
Identity = 92/354 (25.99%), Postives = 165/354 (46.61%), Query Frame = 3 
Query:  246 ISQRDKMKLVEAME-KGENFHALGKQMGIAKSTINSIIYLYNQTGKVTR-GKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQ---QNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENG--ERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTKLNE-----TGFILVHDNVRIY-----DENISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNIQGFVSMNVNNRFIILNDAINKELKKEDY--------------SDLKSYYKHIRKFFPKSLLKE 1214
            IS  D+ +LVEA E  G+++  L   +GI +ST  SI+  Y  +G++ +  + G +N+KI EEM   L + ID N   TL +M   L +   Q   +S  +I+R L    +T +        +N+  VL+ R  Y    LE G      ++IDEC +N W R+T GR+  G   R  V        N+   ++  V G +HH++   ++ + S++ F+     + NE        +L++DN R +      E+++   +  +LPPYS FLN  E      K  ++++++ +  +    V +R     + +N +  + D               +    +Y H + + P+ L ++
Sbjct:   10 ISNVDRRRLVEAFEGNGQDYLELADNLGIKRSTARSIVATYLDSGRIEKLPRGGARNSKIDEEMKNYLQDIIDTNPLLTLRQMKDKLREDLPQKPEVSTTTIARALDVMLMTLKLAEDVPDARNAPRVLQQRGEYAEWFLEHG-VLANCVFIDECGYNIWTRRTQGRAPRGVPARRVVHGQRGRNCNVSFAVSAAV-GLVHHEMAFETVTRASFERFLAAKVAECNEMFPDDAPVVLIYDNARPHVRAELPEDVNSLIETKRLPPYSPFLNMTEMAHSSFKSAVKRTLA-LPAW-QRRVGDRVAAGREGVNLQQWRCDTLREVAVQCIDTVTPAKCTQWYNHSQTYMPRCLERQ 359
[Close]
BLAST of DDE_3 domain-containing protein vs. TrEMBL
Match: A0A1I8GL13 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 3.414e-28
Identity = 84/291 (28.87%), Postives = 149/291 (51.20%), Query Frame = 3 
Query:  237 RKTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTR-GKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQ---QNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVPTTVGPNICLVLAMG-VNGPIHHKLLIGSLAKESYQTFINEVTTKLN----ETGFILVHDNVRIYDE----NISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNIQ 1070
            R+ +S  D+ +L+ A +KGE+F  L  Q+GI ++T  SI+    ++G+V +    G KN K   EM + L   +++N   TL+EM+  L Q   +  H+S  ++S  L     T +  R    ++NS  V+  R+ + + +     S+ + ++IDE  FNTW R++ GRS  G+R++ +V    G  I + LA+    G +H +     + KE +  F+       +    ++   +V DNV  +++     +    ++ +LPPYS FL P+E      K  L++ +S IQ
Sbjct:  889 RQPVSNHDRQRLLSAYQKGEDFILLASQLGIKRTTAYSIV----RSGRVEKLSCGGAKNVKWDGEMQEYLCAIVEQNPLLTLKEMNTMLRQAFPEKQHVSEKTLSIKLDGALFTTKLARDLPAERNSPRVVAAREQFAQWMCSPEASDTEKIFIDEFGFNTWMRRSYGRSLRGQRVYRQVSGQKGQRITVCLAVSPAAGIVHFQSYDSGMTKERFGEFLTITIANFHSVFPDSSISVVIDNVSSHNDAESYEVEDAVEVRRLPPYSPFLTPVENAISCWKAALKRRLSAIQ 1175
[Close]
BLAST of DDE_3 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000044521.1 (pep primary_assembly:Astyanax_mexicanus-2.0:22:28076125:28077147:1 gene:ENSAMXG00000034360.1 transcript:ENSAMXT00000044521.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 9.106e-7
Identity = 64/294 (21.77%), Postives = 134/294 (45.58%), Query Frame = 3 
Query:  231 PVRKTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTRGKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQQNIHISIYSISRFLKKNFVTFRKVRSC--IVDKNSDSVLELRKTYVRRLLEQG----WSNKKTL----YIDECYFNTWARKTTGRSENGERIFVKVPTT--VGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFI-NEVTTKLNETG----FILVHDN--------VRIYDENISPNNQMVKLPPYSSFLNPIEEVFIKIK 1037
            P RK IS   + K+V+   +GE + A+ K+  ++++ +  II  Y +T  V       +  KIS+ + +K++  ++KN  ++ + +   L+   + +S  ++ R L +  +   + R    ++ ++  + L   +  ++   E      WS++  L    ++D  +   W RK  G + N +     VPT    G +I L      +G  +   + G++ KE Y   + N + T  +  G    ++   DN        V+ + ++   N  +++ P  S  LNPIE ++ ++K
Sbjct:    2 PKRKEISLDLRQKIVDLHFQGEGYTAISKRFMVSRTAVRCIIAKYKETKSVQNKHGRGRKRKISKTLQRKIIRDVNKNPWTSAKMIVSELATSGLDVSRKTVVRALHRGGLRGHRPRKTPLLLQRHIKARLNFARDNLKHKSEFWKSVLWSDETKLEQFGHMDAAF--VWRRK--GEAFNPKNT---VPTVKHAGGSIMLWGCFAASGTGNLVRVHGTMKKEDYVDILKNNIRTSASSLGLGRRWVFQQDNDPKHTSKLVKTFLKDTKIN--VLEWPSQSPDLNPIENLWRELK 286
[Close]
BLAST of DDE_3 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000031052.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000043.1:1178862:1180221:-1 gene:ENSAMXG00000033528.1 transcript:ENSAMXT00000031052.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 1.181e-6
Identity = 66/295 (22.37%), Postives = 122/295 (41.36%), Query Frame = 3 
Query:  240 KTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTRGKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQQNIHISIYSISRFLKKNFVTFRKVR--SCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFN---------TWARKTTGRSENGERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEV------------TTKLNETGFILVHDN-----VRIYDENISPNN-QMVKLPPYSSFLNPIEEVFIKIK 1037
            + +S R+K  +++  ++G++  A+GK +GIA +TI +++     TG ++  +   +  K +    ++++  + KN  +T+ +++  L +  + +S  ++ R L++        R    I  KN  + LE  + Y R   +  W+    L+ DE   N          W +K T +        VK     G N+     M  +G     + I ++  +      +EV             TKL    FIL  DN      +   E I     + +  P  S  LNPIE  F  +K
Sbjct:    4 RELSMREKQAILKHRKEGKSIRAIGKTLGIATTTIWNVLKKKETTGVLSNRRRTGRPRKTTPTDERRIVLAVKKNPKTTVSQITSDLHRAGVKVSQSTVRRRLREQTYGGHTARCKPLISSKNRKARLEFARKY-RDEPQTFWNT--VLWTDETKINLYQSDGKAKVWRKKGTAQDPRHTSSSVK---HGGGNVMAWACMAASG-TGSIIFIDNVTDDGSSKINSEVYRQVLSANVRRNATKLIGRSFILQQDNDPKHTAKKTKEFIRGKKWKTLDWPSQSPDLNPIEHAFYLLK 291
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000041908.1 (SMESG000041908.1)

HSP 1 Score: 347.436 bits (890), Expect = 6.541e-108
Identity = 165/322 (51.24%), Postives = 231/322 (71.74%), Query Frame = 3 
Query:  237 RKTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTRGKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQQNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTKLNETGFILVHDNVRIYDENISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNIQGFVSMNVNNRFIILNDAINKELKKEDYSDLKSYYKHIRKFFPKS 1202
            RK IS+ DK++++ AME GE++   GK +GIA+++I +IIY Y + GK+++GK G   +KISEE   KLL+F++KN  ++LEEM  +L  +N+ +S  +ISRFL+ + +T +++R  + ++NSD VL+LR  Y  R LE+ W + + +YIDEC FNTW  +  GR++ GER++  +PT  G N+ L LA+GVNGP+HHKL++GS  K++YQ FINE+T KLN TGF L+HDN  I+   IS NN +  LPPYS FLNPIE VF KIK N+RKS+S   G  SMN  +RF  L DA++ EL KEDY DL +Y+ HIR+FFPKS
Sbjct:  505 RKKISEIDKIRIINAMENGEDYFHFGKLLGIARNSIKTIIYKYRKFGKLSQGKIGGSISKISEENGNKLLDFVEKNVYASLEEMKNFLISENLSVSTNTISRFLENHLITTKQIRPSVAERNSDRVLDLRMEYTTRYLEEAWMDSQLIYIDECGFNTWTSQKLGRNKKGERLYATLPTNRGRNMSLALAIGVNGPVHHKLVVGSYKKDTYQIFINEITVKLNGTGFRLIHDNASIHGGAISLNNVIEHLPPYSPFLNPIESVFSKIKFNIRKSISRAGGLSSMNFQSRFDFLKDALDNELVKEDYRDLTNYFWHIRRFFPKS 826
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000005064.1 (SMESG000005064.1)

HSP 1 Score: 331.643 bits (849), Expect = 5.562e-103
Identity = 159/336 (47.32%), Postives = 227/336 (67.56%), Query Frame = 3 
Query:  186 ISDASSDETLPSKEKPVRKTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTRGKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQQNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTKLNETGFILVHDNVRIYDENISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNIQGFVSMNVNNRFIILNDAINKELKKEDYSDLKSYYKHIRKFF 1193
            +S  +   T P         IS+ DK++++ AME GE++   GK +GI++++I +IIY Y +  K+++GK G   +KISEE   KLL+F++KN  ++L EM  +L  +N+ +S  +ISRFL  + +T +++R  + ++NSD VL+ R  Y  R LE+ W + + +YIDEC FNTW  +  GR++ GER++  +PT  G N+ L LA GVNGP+HHKL++GS  K++YQ FINE+T KLN TG+ L+HDN  I+   IS NN +  LPPYS FLN IE VF KIK N+RKS+S   GF SMN  +RF  L DA++ EL KEDYSDL +Y+ HIR+FF
Sbjct:  110 LSKVARPSTTPETLTKKHTLISEIDKIRIINAMENGEDYFYFGKLLGISRNSIKTIIYKYRKFEKLSQGKIGGNKSKISEEHGNKLLDFVEKNVYASLVEMKNFLISENLSVSTNTISRFLGNHLITTKQIRPSVAERNSDRVLDFRMKYTTRYLEEAWMDSQLIYIDECGFNTWTSQKLGRNKKGERLYATLPTNRGRNMSLALAFGVNGPVHHKLVVGSYKKDTYQIFINEITVKLNGTGYRLIHDNASIHGGAISLNNAIEHLPPYSPFLNLIESVFSKIKFNIRKSISRAGGFSSMNFQSRFDFLKDALDNELVKEDYSDLTNYFGHIRRFF 445
[Close]
BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000005064.1 (SMESG000005064.1)

HSP 1 Score: 302.753 bits (774), Expect = 1.533e-92
Identity = 146/317 (46.06%), Postives = 211/317 (66.56%), Query Frame = 3 
Query:  186 ISDASSDETLPSKEKPVRKTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTRGKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQQNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTKLNETGFILVHDNVRIYDENISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNIQGFVSMNVNNRFIILNDAINKEL 1136
            +S  +   T P         IS+ DK++++ AME GE++   GK +GI++++I +IIY Y +  K+++GK G   +KISEE   KLL+F++KN  ++L EM  +L  +N+ +S  +ISRFL  + +T +++R  + ++NSD VL+ R  Y  R LE+ W + + +YIDEC FNTW  +  GR++ GER++  +PT  G N+ L LA GVNGP+HHKL++GS  K++YQ FINE+T KLN TG+ L+HDN  I+   IS NN +  LPPYS FLN IE VF KIK N+RKS+S   GF SMN  +RF  L DA++ EL
Sbjct:   93 LSKVARPSTTPETLTKKHTLISEIDKIRIINAMENGEDYFYFGKLLGISRNSIKTIIYKYRKFEKLSQGKIGGNKSKISEEHGNKLLDFVEKNVYASLVEMKNFLISENLSVSTNTISRFLGNHLITTKQIRPSVAERNSDRVLDFRMKYTTRYLEEAWMDSQLIYIDECGFNTWTSQKLGRNKKGERLYATLPTNRGRNMSLALAFGVNGPVHHKLVVGSYKKDTYQIFINEITVKLNGTGYRLIHDNASIHGGAISLNNAIEHLPPYSPFLNLIESVFSKIKFNIRKSISRAGGFSSMNFQSRFDFLKDALDNEL 409
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BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000069530.1 (SMESG000069530.1)

HSP 1 Score: 268.855 bits (686), Expect = 2.177e-88
Identity = 125/242 (51.65%), Postives = 169/242 (69.83%), Query Frame = 3 
Query:  486 MSKYLSQQNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTKLNETGFILVHDNVRIYDENISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNIQGFVSMNVNNRFIILNDAINKELKKEDYSDLKSYYKHIRKFFPKSLLK 1211
            M  +L  +N+ +S  +ISRFL  + +T +++R  + ++NSD VL+LR  Y  R LE+ W + + +YIDEC FNTW  +  GR++ GER++  +PT  G N+ L LA+GVNGP+HHKL++GS  K++YQ FINE+T KLN TGF L+HDN  I+   IS NN +  LPPYS FLNPIE VF KIK N+RKS+S   G  SMN  +RF  L DA++ EL KEDY DL +Y+ HIR+FFPK + K
Sbjct:    1 MKNFLISENLSVSTNTISRFLGNHLITTKQIRPSVAERNSDRVLDLRMEYTTRYLEEAWMDSQLIYIDECGFNTWTSQKLGRNKKGERLYATLPTNRGRNMSLALAIGVNGPVHHKLVVGSYKKDTYQVFINEITVKLNGTGFRLIHDNASIHGGAISLNNVIEHLPPYSPFLNPIESVFSKIKFNIRKSISRAGGLSSMNFQSRFDFLKDALDNELVKEDYRDLTNYFWHIRRFFPKCIKK 242
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BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000050992.1 (SMESG000050992.1)

HSP 1 Score: 226.483 bits (576), Expect = 2.526e-65
Identity = 103/232 (44.40%), Postives = 161/232 (69.40%), Query Frame = 3 
Query:  219 SKEKPVRKTISQRDKMKLVEAMEKGENFHALGKQMGIAKSTINSIIYLYNQTGKVTRGKPGTKNTKISEEMPQKLLNFIDKNYCSTLEEMSKYLSQQNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVLELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVPTTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTKLNETGF 914
            S    V + IS+ DK++++ AME GE++   GK +GI +++I ++IY Y + GK+++GK G   +KISE+   KLL+F++KN  ++LEEM  +L  +N+ +S  +ISRFL  + +T +++R  + ++NSD VL+LR  Y  R LE+ W + + +YIDEC FN W  +  GR++ GER++  +PT  G N+ L LA+GVNG +HHKL++GS  K++YQ FINE+T KLN TG+
Sbjct:  956 SSSGGVTRRISEIDKIRIINAMENGEDYFHFGKLLGIFRNSIKTLIYKYRKFGKLSQGKIGGSKSKISEDYGNKLLDFVEKNVYASLEEMKNFLIFENLSVSTNTISRFLGNHLITTKQIRPSVAERNSDRVLDLRMKYTTRYLEEAWMDSQLIYIDECGFNIWTSQKLGRNKKGERLYSTLPTNRGRNMSLALAIGVNGSVHHKLIVGSYKKDTYQIFINEITVKLNGTGY 1187
The following BLAST results are available for this feature:
BLAST of DDE_3 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000000571.12.288e-822.52pep primary_assembly:Xenopus_tropicalis_v9.1:1:804... [more]
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BLAST of DDE_3 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0C2JI121.939e-3228.19DDE_3 domain-containing protein OS=Thelohanellus k... [more]
A0A267DQJ81.147e-3127.74DDE_3 domain-containing protein OS=Macrostomum lig... [more]
W4XP444.795e-3026.14DDE_3 domain-containing protein OS=Strongylocentro... [more]
W4Z6F77.295e-2925.99DDE_3 domain-containing protein OS=Strongylocentro... [more]
A0A1I8GL133.414e-2828.87Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of DDE_3 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000044521.19.106e-721.77pep primary_assembly:Astyanax_mexicanus-2.0:22:280... [more]
ENSAMXT00000031052.11.181e-622.37pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of DDE_3 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DDE_3 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041908.16.541e-10851.24SMESG000041908.1[more]
SMESG000005064.15.562e-10347.32SMESG000005064.1[more]
SMESG000005064.11.533e-9246.06SMESG000005064.1[more]
SMESG000069530.12.177e-8851.65SMESG000069530.1[more]
SMESG000050992.12.526e-6544.40SMESG000050992.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010233 ID=SMED30010233|Name=DDE_3 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1253bp
AATGAATTGCGATTTGGTGTACAGGAGTCAATAACAAACACACAAACATA
CTAAACATACAAACACAATGATGTGTGTTAAATATGAATGCATATGATTG
CAAACCTGCGTTTTTGATTTGAAAACATTAATTTTAAATTGTAAATATTT
TTTGAAGAATTATTACAATGGATGCATGTAAAGAAATATCCGATGCATCG
AGTGATGAAACATTGCCTTCCAAAGAAAAACCTGTTAGAAAAACAATATC
ACAAAGGGATAAAATGAAACTTGTTGAGGCCATGGAAAAAGGCGAAAACT
TTCATGCACTGGGAAAACAAATGGGTATTGCCAAAAGTACAATAAATAGT
ATAATTTATTTATATAATCAAACTGGGAAGGTGACCAGAGGAAAACCGGG
AACCAAAAACACAAAGATTTCAGAAGAAATGCCACAAAAACTGCTGAATT
TCATTGATAAAAATTATTGCTCAACTTTAGAAGAAATGAGCAAATATTTA
TCTCAGCAAAATATTCATATTTCAATATATTCCATATCACGATTCCTAAA
AAAGAATTTCGTCACTTTCAGAAAAGTTCGATCATGTATTGTAGATAAAA
ATTCAGATAGTGTTTTAGAACTTCGAAAAACATACGTTCGACGATTGTTA
GAACAAGGATGGAGTAACAAGAAAACACTTTATATTGACGAATGTTATTT
CAATACATGGGCCAGGAAAACTACTGGCAGAAGCGAAAATGGAGAAAGGA
TATTCGTAAAAGTTCCAACAACTGTTGGTCCAAATATTTGTCTAGTGCTC
GCCATGGGAGTGAACGGACCGATTCATCACAAATTACTAATTGGGTCTTT
GGCCAAAGAGAGTTATCAAACTTTTATCAATGAGGTAACTACCAAATTGA
ATGAAACAGGTTTCATTCTCGTCCACGACAATGTTCGAATTTATGATGAA
AATATTTCTCCAAATAACCAAATGGTAAAACTTCCTCCTTATAGTTCCTT
TCTCAATCCAATTGAAGAGGTTTTTATCAAAATCAAAGAAAACCTCAGAA
AATCTATGTCCAACATCCAGGGATTCGTTTCAATGAATGTCAATAATCGA
TTTATTATTCTGAACGATGCTATCAACAAAGAGCTAAAGAAAGAAGATTA
TTCTGATTTAAAAAGTTATTATAAGCATATACGAAAATTTTTCCCAAAAT
CCCTTTTAAAGGAAGTAAATTATGAAGATGAGTAATTTTATGAATAAATG
TAC
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protein sequence of SMED30010233-orf-1

>SMED30010233-orf-1 ID=SMED30010233-orf-1|Name=SMED30010233-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=356bp
MDACKEISDASSDETLPSKEKPVRKTISQRDKMKLVEAMEKGENFHALGK
QMGIAKSTINSIIYLYNQTGKVTRGKPGTKNTKISEEMPQKLLNFIDKNY
CSTLEEMSKYLSQQNIHISIYSISRFLKKNFVTFRKVRSCIVDKNSDSVL
ELRKTYVRRLLEQGWSNKKTLYIDECYFNTWARKTTGRSENGERIFVKVP
TTVGPNICLVLAMGVNGPIHHKLLIGSLAKESYQTFINEVTTKLNETGFI
LVHDNVRIYDENISPNNQMVKLPPYSSFLNPIEEVFIKIKENLRKSMSNI
QGFVSMNVNNRFIILNDAINKELKKEDYSDLKSYYKHIRKFFPKSLLKEV
NYEDE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR038717Tc1-like_DDE_dom
IPR009057Homeobox-like_sf
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038717Tc1-like transposase, DDE domainPFAMPF13358DDE_3coord: 170..296
e-value: 7.8E-11
score: 41.9
NoneNo IPR availableGENE3DG3DSA:3.30.420.470coord: 135..342
e-value: 4.7E-7
score: 31.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availablePANTHERPTHR23022:SF111coord: 18..300
NoneNo IPR availablePANTHERPTHR23022TRANSPOSABLE ELEMENT-RELATEDcoord: 18..300
IPR009057Homeobox-like domain superfamilySUPERFAMILYSSF46689Homeodomain-likecoord: 24..129