tolloid like-1

Overview
Nametolloid like-1 (AFJ24777.1)
Smed IDSMED30010228
Length (bp)814
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tolloid like-1 (SMED30010228) t-SNE clustered cells

Violin plots show distribution of expression levels for tolloid like-1 (SMED30010228) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010228

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30010228h1SMcG0009028 SmedASXL_004157SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30010228h1SMcG0009028 SmedASXL_004157SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30010228h1SMcG0009028 OX_Smed_1.0.22104ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tolloid like-1 vs. Ensembl Human
Match: ASTL (astacin like metalloendopeptidase [Source:HGNC Symbol;Acc:HGNC:31704])

HSP 1 Score: 88.1965 bits (217), Expect = 2.401e-19
Identity = 59/215 (27.44%), Postives = 98/215 (45.58%), Query Frame = 1 
Query:  118 LLSKADIAIINGFKHHQREARAWPGG-----KVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCM-NSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTM--IQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
             L + DI   + F+     +  WP G     +V F  ++ +    R ++ + L E +     C++F                GC++ +G +G   Q ++L  P C+   +G + HE +H LGF H H R DRD +I +NW  I      NF+K +   M   Y+  SVMHY   AF  ++G+ T+  +      I    N + +D++ +  LY C
Sbjct:   72 FLIEGDIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERS--TCIRFVTYQDQRDFISIIPMYGCFSSVGRSGGM-QVVSLA-PTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAF-SRRGLPTITPLWAPSVHIGQRWNLSASDITRVLKLYGC 281
[Close]
BLAST of tolloid like-1 vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 87.8113 bits (216), Expect = 3.215e-19
Identity = 55/199 (27.64%), Postives = 92/199 (46.23%), Query Frame = 1 
Query:  169 REARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGN-----LIDYNHNTTPNDLSMIKSLYNC 738
            R  R WPGG + +    +F   +R + KQ +   +     CV F E S +  +I    +  GC + +G  G   Q +++      +  G + HE  H +GF H H R DRDN ++I   NIQ  Q +NF+K E   ++     Y+  S+MHY+ + F     ++T++    +      I      +  D++  + LY C
Sbjct:  152 RTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGK--NCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of tolloid like-1 vs. Ensembl Human
Match: BMP1 (bone morphogenetic protein 1 [Source:HGNC Symbol;Acc:HGNC:1067])

HSP 1 Score: 86.2705 bits (212), Expect = 1.800e-18
Identity = 57/199 (28.64%), Postives = 92/199 (46.23%), Query Frame = 1 
Query:  169 REARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFEST-QGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQK---NGNLIDYNHNT--TPNDLSMIKSLYNC 738
            R  R WP G + F    +F   +R + +Q +   +     CV F E T +  +I    +  GC + +G  G   Q +++      +  G + HE  H +GF H H R DRD  +SI   NIQ  Q +NF+K E   ++     Y+  S+MHY+ + F     ++T++ K   NG        T  +  D++  + LY C
Sbjct:  125 RPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKN--CDKFGIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKC 319
[Close]
BLAST of tolloid like-1 vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 86.2705 bits (212), Expect = 2.001e-18
Identity = 55/199 (27.64%), Postives = 92/199 (46.23%), Query Frame = 1 
Query:  169 REARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGN-----LIDYNHNTTPNDLSMIKSLYNC 738
            R  R WPGG + +    +F   +R + KQ +   +     CV F E S +  +I    +  GC + +G  G   Q +++      +  G + HE  H +GF H H R DRDN ++I   NIQ  Q +NF+K E   ++     Y+  S+MHY+ + F     ++T++    +      I      +  D++  + LY C
Sbjct:  152 RTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKN--CDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of tolloid like-1 vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 86.2705 bits (212), Expect = 2.010e-18
Identity = 55/199 (27.64%), Postives = 92/199 (46.23%), Query Frame = 1 
Query:  169 REARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGN-----LIDYNHNTTPNDLSMIKSLYNC 738
            R  R WPGG + +    +F   +R + KQ +   +     CV F E S +  +I    +  GC + +G  G   Q +++      +  G + HE  H +GF H H R DRDN ++I   NIQ  Q +NF+K E   ++     Y+  S+MHY+ + F     ++T++    +      I      +  D++  + LY C
Sbjct:  152 RTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKN--CDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of tolloid like-1 vs. Ensembl Celegans
Match: nas-14 (Zinc metalloproteinase nas-14 [Source:UniProtKB/Swiss-Prot;Acc:Q19269])

HSP 1 Score: 94.3597 bits (233), Expect = 9.820e-22
Identity = 62/194 (31.96%), Postives = 92/194 (47.42%), Query Frame = 1 
Query:  178 RAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNK---GCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGNL-IDYNHNTTPNDLSMIKSLYNC 738
            + WP G+V +        D+R  + Q  +E ++    CV+F   T      ++ +     GC + +G  G   Q ++L+   C  SKG I HE +HALGF H H R DRD+F+ IN  NI+     NF K    I+D     Y+  SVMHY   AF  + G  T+I K+    +   +  +  D   +  LY C
Sbjct:  123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKT--KTCVRFVPKTDDDFDYIYVKRNVAFGCSSYVGRAG-GNQTVSLEVDKCF-SKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFS-RNGKPTIIPKDNEADVGQRYKLSEMDSKKVNKLYQC 311
[Close]
BLAST of tolloid like-1 vs. Ensembl Celegans
Match: nas-7 (Zinc metalloproteinase nas-7 [Source:UniProtKB/Swiss-Prot;Acc:P55113])

HSP 1 Score: 93.2041 bits (230), Expect = 1.113e-21
Identity = 67/215 (31.16%), Postives = 102/215 (47.44%), Query Frame = 1 
Query:  127 KADIAIINGFKHH--QREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQG-PHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCE---GCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKN-----GNLIDYNHNTTPNDLSMIKSLYNC 738
            K+DI +    K +   R A+ WP  ++ +A +  +   +R L+ + + +       C++F     G P      +  GC++ +G T    Q L+L N  CM    TI HE +H +GF H H R DRDNFI I W+NI       F K +          Y+ +S++HY S AF  + G  TM+ K      GN  D++      D+S I  +YNC
Sbjct:   68 KSDIRLPRRHKRNGVSRAAKLWPNARIPYAISPHYSPHERALLAKAVKQYHE--KTCIRFVPRQTGEPDYLFIGKVDGCFSEVGRTSG-VQVLSLDN-GCMEY-ATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFS-KNGFPTMLPKVKSATIGNARDFSD----VDISKINRMYNC 272
[Close]
BLAST of tolloid like-1 vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 87.4261 bits (215), Expect = 7.169e-20
Identity = 56/195 (28.72%), Postives = 98/195 (50.26%), Query Frame = 1 
Query:  178 RAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPH---IAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCI---MDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSM--IKSLYNC 738
            R WP  ++ +  ++ + +  R+++   +NE  +    CV+F       H   + +H  ++GCY+++G TG + Q ++L +  C+   GTI HE +HA+GF H   R DRD++I + W+N+ +     F K    +   +D  Y+  S+MHY   AF    G  T++ K             +D+ +  I  LYNC
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGGK-QPVSLDS-GCIQV-GTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSG-SGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of tolloid like-1 vs. Ensembl Celegans
Match: nas-27 (Zinc metalloproteinase nas-27 [Source:UniProtKB/Swiss-Prot;Acc:O17264])

HSP 1 Score: 88.1965 bits (217), Expect = 9.460e-20
Identity = 70/237 (29.54%), Postives = 106/237 (44.73%), Query Frame = 1 
Query:   85 RATENLHVGDM--------LLSKADIAIINGFKHH---QREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQG-PHIAVHAQNKGCYAMIGCTGE-RGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMD---GNYETRSVMHYSSDAFGCQQGMNTMI---QKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            RA  N + GD+         L + DIA++         +++ + W    + ++F  +FP+  R  V + +         CV F E+    PH+++   N GC++ +G     R Q L+L+   C +    + HE  H LGF H H R DRD FISI++ N+  +  F F K     +D     YE  SVMHYS D F        +    QK    +      T  D+S +  LYNC
Sbjct:   23 RAVRNRNEGDINSLVGVGQYLYQGDIAVVKSRARRAVIRQKHKKW-KLPMPYSFDRNFPSRSRQRVLEAMQFWSEK--TCVTFHENRYVYPHVSIFEGN-GCWSFVGKQPSLREQSLSLERS-CTDHTFVVAHEIAHTLGFYHEHARGDRDQFISIDYSNVNPNLTFAFAKESEKQLDHQEAAYEYGSVMHYSVDQFAVNTNRPVIYARDQKFAQAMGNRMRATFQDVSRMNVLYNC 254
[Close]
BLAST of tolloid like-1 vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 86.6557 bits (213), Expect = 2.100e-19
Identity = 56/195 (28.72%), Postives = 98/195 (50.26%), Query Frame = 1 
Query:  178 RAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPH---IAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCI---MDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSM--IKSLYNC 738
            R WP  ++ +  ++ + +  R+++   +NE  +    CV+F       H   + +H  ++GCY+++G TG + Q ++L +  C+   GTI HE +HA+GF H   R DRD++I + W+N+ +     F K    +   +D  Y+  S+MHY   AF    G  T++ K             +D+ +  I  LYNC
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGGK-QPVSLDSG-CIQV-GTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFS-GSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of tolloid like-1 vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0084378)

HSP 1 Score: 117.087 bits (292), Expect = 1.877e-30
Identity = 71/222 (31.98%), Postives = 114/222 (51.35%), Query Frame = 1 
Query:  109 GDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG-NYETRSVMHYSSDAFGC----------QQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            GDML+ + D+ + NG      ++  WP G V +    +F A     ++  + E       C++F + S++  +I++   N GC++ +G  G + Q +NLQ+P C++  GT  HE +HALGF+H   RM+RD +++I + N+QSS   NF K       G  Y+  SVMHYS +AF              G + M Q+NG   DY       D+  +  +Y+C
Sbjct:   95 GDMLVPQTDLIMKNGLP---TQSSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGGK-QEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNG-FSDY-------DIQKLNRMYDC 302
[Close]
BLAST of tolloid like-1 vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0334497)

HSP 1 Score: 117.087 bits (292), Expect = 1.877e-30
Identity = 71/222 (31.98%), Postives = 114/222 (51.35%), Query Frame = 1 
Query:  109 GDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG-NYETRSVMHYSSDAFGC----------QQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            GDML+ + D+ + NG      ++  WP G V +    +F A     ++  + E       C++F + S++  +I++   N GC++ +G  G + Q +NLQ+P C++  GT  HE +HALGF+H   RM+RD +++I + N+QSS   NF K       G  Y+  SVMHYS +AF              G + M Q+NG   DY       D+  +  +Y+C
Sbjct:   95 GDMLVPQTDLIMKNGLP---TQSSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGGK-QEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNG-FSDY-------DIQKLNRMYDC 302
[Close]
BLAST of tolloid like-1 vs. Ensembl Fly
Match: CG5715 (gene:FBgn0039180 transcript:FBtr0084634)

HSP 1 Score: 92.8189 bits (229), Expect = 6.929e-22
Identity = 67/224 (29.91%), Postives = 108/224 (48.21%), Query Frame = 1 
Query:  103 HVGDML--LSKADIAIINGFKHHQREARA-WPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQF-FESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTM----IQKNGNLIDYNHNTTPNDLSMIKSLYNCR 741
            H GD+L  LS  D A  NG ++      + WPGG V +     F + +   +     E  +    CV+F   +T+  +I++ +   GC++ IG  G R Q +NLQ+P C+ + GT  HE +HALGF H   R +RD+++ +   NI+     NF K       G    Y+  SVMHYS  +F  + G  T+       + + +      +  D+  I ++Y C+
Sbjct:   76 HEGDILIPLSYRD-ARFNGTRNGILALSSRWPGGVVPYEIKGPFTSQELGNINHAFKEYHTK--TCVRFKPRTTEKDYISIGSGKSGCWSSIGRLGGR-QEVNLQSPNCLRTYGTPIHELMHALGFFHEQNRHERDSYVRVMKDNIKPEMMVNFEKSSSRTQYGFGVEYDYGSVMHYSPTSF-TRNGQPTLKALRATSDASQMGQRKGFSAGDVRKINAMYKCK 294
[Close]
BLAST of tolloid like-1 vs. Ensembl Fly
Match: CG11865 (gene:FBgn0028947 transcript:FBtr0080753)

HSP 1 Score: 90.1225 bits (222), Expect = 3.344e-21
Identity = 58/189 (30.69%), Postives = 97/189 (51.32%), Query Frame = 1 
Query:  184 WPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQF--FESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNF--VKCEGCIMDG-NYETRSVMHYSSDAFGCQQGMNTMIQ-KNGNLIDYNHNTTPNDLSMIKSLY 732
            W G  + +++A  F +     ++  + E+ S    CV+F   E  + P + +  +  GC++ +G  G   Q LNL +  CM+++ TI+HE +HALGF HTH    RD ++ I   NI+S    NF  ++  G    G  Y+  S+MHY   AF  + G +T++  K+   I      +P D+  +K +Y
Sbjct:   56 WKGRTLVYSYAGGFSSLDIASIESAMAEISSK--TCVKFRRTEYKREPQVVIQKEGSGCWSYVGYLGRADQTLNLGSG-CMSNR-TIQHELLHALGFFHTHSDPQRDKYVRIQTDNIRSGHEHNFQRLRANGVTNYGFGYDYDSIMHYGPFAF-SKNGQSTIVPLKSHAKIGQATQMSPKDVQTLKRMY 239
[Close]
BLAST of tolloid like-1 vs. Ensembl Fly
Match: CG15255 (gene:FBgn0028950 transcript:FBtr0080785)

HSP 1 Score: 86.2705 bits (212), Expect = 1.052e-19
Identity = 58/197 (29.44%), Postives = 98/197 (49.75%), Query Frame = 1 
Query:  178 RAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQ--GPHIAVHAQNKGCYAMIGCTGERGQFLNLQ----NPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIM---DGNYETRSVMHYSSDAFGCQQGMNTMIQKNGN-LIDYNHNTTPNDLSMIKSLYNC 738
            + WPG  V +  ++DF    +  ++  ++ L+  L  C++F E+T     ++ V A++ GCY  +G  G   Q +NL+       C    GTI HEF+HALGF H      RD+FI++ + NI   + FNF K    ++   +  Y+  S +HY   AF    G +T++  + + +I      +  D+  I  +Y C
Sbjct:   66 KRWPGNVVVYRISDDFDTAHKKAIQTGIDTLE--LHTCLRFREATDEDKAYLTVTAKSGGCYTAVGYQGAP-QEMNLEIYPLGEGCFRP-GTILHEFMHALGFYHQQSSSIRDDFINVIYENIVPGKEFNFQKYADTVVTDFEVGYDYDSCLHYRPGAFSI-NGEDTIVPLDSSAVIGQRVGLSSKDIDKINIMYKC 257
[Close]
BLAST of tolloid like-1 vs. Ensembl Zebrafish
Match: hce2l1 (high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:ZDB-GENE-070912-147])

HSP 1 Score: 93.5893 bits (231), Expect = 1.251e-22
Identity = 59/153 (38.56%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:  286 NCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPN--DLSMIKSLYNC 738
             CV+F   T   +        GC++ +G TG   Q ++LQ+P CM S G   HE +HALGF+H   R DRD ++SI W NI  +Q  NF K E   ++  Y+  SVMHY   AF  + G  T+I K    I       P+  D+  I  LYNC
Sbjct:   66 TCVKFVPRTHQANFLNIQPRYGCWSYLGMTGG-SQTVSLQSPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVSILWENIIENQRHNFRKYETNNLNTAYDYSSVMHYGRYAFS-EDGGPTIIPKPDPYIPIGQRDGPSILDIHKINILYNC 215
[Close]
BLAST of tolloid like-1 vs. Ensembl Zebrafish
Match: he1.2 (hatching enzyme 1, tandem dupliate 2 [Source:ZFIN;Acc:ZDB-GENE-030131-2100])

HSP 1 Score: 93.9745 bits (232), Expect = 2.062e-22
Identity = 65/220 (29.55%), Postives = 109/220 (49.55%), Query Frame = 1 
Query:   88 ATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFF-ESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTM--IQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            ++E L  GD++L K   A+I   K    +  A    +V +  + +F  + ++++   ++   +    C++F   S Q  ++++  ++ GCY+ IG TG + Q ++L    C+ S G  +HE  HALGF H   R DRD ++ INW NI     +NF+K +    +  Y+  S+MHY   AF  Q G+ T+  I      I      +  D+  I  LY C
Sbjct:   49 SSEVLFEGDVVLPKNRNALICEDKSCFWKKNANNIVEVPYVVSGEFSINDKSVIANAISIFHAQ--TCIRFVPRSIQADYLSIENKD-GCYSAIGRTGGK-QVVSLNRKGCVYS-GIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGLSKIDILRINKLYGC 263
[Close]
BLAST of tolloid like-1 vs. Ensembl Zebrafish
Match: hce2l1 (high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:ZDB-GENE-070912-147])

HSP 1 Score: 93.9745 bits (232), Expect = 3.074e-22
Identity = 59/153 (38.56%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:  286 NCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPN--DLSMIKSLYNC 738
             CV+F   T   +        GC++ +G TG   Q ++LQ+P CM S G   HE +HALGF+H   R DRD ++SI W NI  +Q  NF K E   ++  Y+  SVMHY   AF  + G  T+I K    I       P+  D+  I  LYNC
Sbjct:  124 TCVKFVPRTHQANFLNIQPRYGCWSYLGMTGG-SQTVSLQSPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVSILWENIIENQRHNFRKYETNNLNTAYDYSSVMHYGRYAFS-EDGGPTIIPKPDPYIPIGQRDGPSILDIHKINILYNC 273
[Close]
BLAST of tolloid like-1 vs. Ensembl Zebrafish
Match: he1.1 (hatching enzyme 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-021211-3])

HSP 1 Score: 93.2041 bits (230), Expect = 4.502e-22
Identity = 65/220 (29.55%), Postives = 108/220 (49.09%), Query Frame = 1 
Query:   88 ATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFF-ESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTM--IQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            ++E L  GD++L K   A I   K    +  A    +V +  + +F  + ++++   ++   +    C++F   S Q  ++++  ++ GCY+ IG TG + Q ++L    C+ S G  +HE  HALGF H   R DRD ++ INW NI     +NF+K +    +  Y+  S+MHY   AF  Q G+ T+  I      I      +  D+  I  LY C
Sbjct:   49 SSEVLFEGDVVLPKNRNAFICEDKSCFWKKNANNIVEVPYVVSGEFSINDKSVIANAISIFHAQ--TCIRFVPRSIQADYLSIENKD-GCYSAIGRTGGK-QVVSLNRKGCVYS-GIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGLSKIDILRINKLYGC 263
[Close]
BLAST of tolloid like-1 vs. Ensembl Zebrafish
Match: he1.1 (hatching enzyme 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-021211-3])

HSP 1 Score: 93.2041 bits (230), Expect = 4.502e-22
Identity = 65/220 (29.55%), Postives = 108/220 (49.09%), Query Frame = 1 
Query:   88 ATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFF-ESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTM--IQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            ++E L  GD++L K   A I   K    +  A    +V +  + +F  + ++++   ++   +    C++F   S Q  ++++  ++ GCY+ IG TG + Q ++L    C+ S G  +HE  HALGF H   R DRD ++ INW NI     +NF+K +    +  Y+  S+MHY   AF  Q G+ T+  I      I      +  D+  I  LY C
Sbjct:   49 SSEVLFEGDVVLPKNRNAFICEDKSCFWKKNANNIVEVPYVVSGEFSINDKSVIANAISIFHAQ--TCIRFVPRSIQADYLSIENKD-GCYSAIGRTGGK-QVVSLNRKGCVYS-GIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGLSKIDILRINKLYGC 263
[Close]
BLAST of tolloid like-1 vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 98.9821 bits (245), Expect = 6.330e-23
Identity = 74/230 (32.17%), Postives = 111/230 (48.26%), Query Frame = 1 
Query:   73 KLMTRATENLHV----GDMLL--SKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQK-NGNL-IDYNHNTTPNDLSMIKSLYNC 738
             LMT+A E   V    GD+ +  S++ I   + F          P     +     + +D+ N +   + E+ S L  CVQF   T            GC++ +G  G   Q ++++  +C  S+GT  HE  HALGF+H H R DRDN+++I ++ I S    NF K E   +D  Y+ RS+MHY + AF    G NT++ K N N+ I   +  T  D+  I  LY C
Sbjct:   45 SLMTKANEGSRVRRVQGDIAVRVSRSTIVCTDCFWQKSNGIVYVP-----YTLDKQYSSDQINTITSAM-EVYSTL-TCVQFVPYTDEEDYIAITSGDGCWSFMGRQGG-AQVVSVEKGYC-TSEGTTMHELNHALGFVHEHSRSDRDNYVNIMYQYISSGDDVNFEKMETNNLDTTYDYRSIMHYPAWAFSNTTGQNTIVAKPNPNVTIGAGNTMTSLDIIKINRLYEC 265
[Close]
BLAST of tolloid like-1 vs. Ensembl Xenopus
Match: kpna4 (karyopherin alpha 4 (importin alpha 3) [Source:Xenbase;Acc:XB-GENE-1011058])

HSP 1 Score: 96.6709 bits (239), Expect = 3.053e-22
Identity = 72/234 (30.77%), Postives = 106/234 (45.30%), Query Frame = 1 
Query:   58 GCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGK-----VSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNH--NTTPNDLSMIKSLYNC 738
            G    K +    +    G+M L++    +IN  +        WP        V + F+ ++ AD+  L K  + E ++    CV+F   T          N GC +M+G  G  GQ + L +  CM S+G I+HE  HALGF H HMR DRD++++I   NI  S    F K +   M   Y+  SVMHYS + F      +T++ K    I        +  D+  IK LY C
Sbjct:   32 GITASKEILEENKKSGPGNMALTEGGDILINIGRSATSSDYLWPKSADGTVVVPYIFSYNYSADELTLFKTAMQEFETL--TCVRFVPKTIQRDFLNIVSNGGCLSMVGRNG-GGQKVELASYGCM-SRGVIQHELNHALGFYHEHMRSDRDDYVTIITENIIPSYENYFSKRKTNNMGIIYDYNSVMHYSRNTFSISPDKSTIVPKPDPSIPIGQRDGLSILDILKIKKLYQC 261
[Close]
BLAST of tolloid like-1 vs. Ensembl Xenopus
Match: polr2l.2 (polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-5898446])

HSP 1 Score: 95.9005 bits (237), Expect = 6.495e-22
Identity = 65/214 (30.37%), Postives = 102/214 (47.66%), Query Frame = 1 
Query:  121 LSKADIAIINGFKHHQREARAWPGG-----KVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNL--IDYNHNTTPNDLSMIKSLYNCR 741
            L + DIAI  G    +  +  WP       +V +  + D+  ++++ ++  L E  +    CV F E +          + GC++ IG TG   Q L+L +  C+ +KG I+HE  HALGF H   R DRD +I + W+ I  S   +F   +   +D  Y+  SVMHY   AF    G  ++  K      I   +  +P D+S +K LY C+
Sbjct:   50 LWQGDIAIETGRSATKCPSCLWPKSADGTVRVPYRLSADYSDNEKSSIRDALLEFNT--LTCVHFVERSTEEDFLDIVSDSGCWSSIGRTG-GAQTLSLMSSGCL-AKGIIQHEVDHALGFYHEQSRSDRDTYIDVLWQYICESDWGSFEMVDTDNLDLPYDYSSVMHYGWYAFSNTSGQPSLRPKPDPTANIGQRYGLSPLDVSKVKELYGCK 259
[Close]
BLAST of tolloid like-1 vs. Ensembl Xenopus
Match: ENSXETT00000022029.1 (astacin-like metalloendopeptidase [Source:NCBI gene;Acc:100496745])

HSP 1 Score: 89.7373 bits (221), Expect = 7.774e-20
Identity = 58/194 (29.90%), Postives = 100/194 (51.55%), Query Frame = 1 
Query:  184 WPGGK-----VSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGN---LIDYNHNTTPNDLSMIKSLYNC 738
            WP  +     V +  +N++ + +R++++  ++E+   +  C+QF   + +  ++ +   + GC++ IG  G   Q ++L    C++  G I+HE +H+LGF H   R DRDN+I+INW NI   +  NF+K     +   Y+  SVMHY   AF    G  T+ +  GN   +I      +  D+  I  LY C
Sbjct:   24 WPRSRKGLVLVPYTLSNNYNSTERDIIRAAMDEVT--VLTCIQFVTYNNESDYLRIRPYD-GCWSYIGRVGG-AQDVSLMKTGCLH-HGVIQHELLHSLGFQHEQCRSDRDNYININWDNISHDKERNFLKMNTQNLGSPYDYLSVMHYGKFAFATNSGKPTL-EPKGNPSAMIGQRVGLSSLDVEKINRLYQC 211
[Close]
BLAST of tolloid like-1 vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 89.7373 bits (221), Expect = 9.554e-20
Identity = 55/152 (36.18%), Postives = 78/152 (51.32%), Query Frame = 1 
Query:  289 CVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQK-NGNL-IDYNHNTTPNDLSMIKSLYNC 738
            CVQF   T            GC++ +G  G   Q +++Q  +C  S+GT  HE  HALGF+H H R DRDN++ I ++ I      NF K E   ++  Y+  S+MHY + AF    G NT++ K N N+ I   +  T  D+  I  LY C
Sbjct:  111 CVQFVPYTDEDDYIAITSGDGCWSYMGRQGG-AQVVSVQKTYC-TSEGTTMHELNHALGFVHEHSRSDRDNYVDIMYQYISPGDIVNFKKMETNNLNTTYDYHSIMHYPAWAFSNTTGQNTIVAKPNPNVTIGAGNTMTSLDIIKINRLYEC 260
[Close]
BLAST of tolloid like-1 vs. Ensembl Mouse
Match: Tll1 (tolloid-like [Source:MGI Symbol;Acc:MGI:106923])

HSP 1 Score: 86.2705 bits (212), Expect = 1.367e-18
Identity = 55/199 (27.64%), Postives = 92/199 (46.23%), Query Frame = 1 
Query:  169 REARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGN-----LIDYNHNTTPNDLSMIKSLYNC 738
            R  R WPGG + +    +F   +R + KQ +   +     CV F E S +  +I    +  GC + +G  G   Q +++      +  G + HE  H +GF H H R DRDN ++I   NIQ  Q +NF+K E   ++     Y+  S+MHY+ + F     ++T++    +      I      +  D++  + LY C
Sbjct:  152 RTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFTERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKN--CDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of tolloid like-1 vs. Ensembl Mouse
Match: Bmp1 (bone morphogenetic protein 1 [Source:MGI Symbol;Acc:MGI:88176])

HSP 1 Score: 84.3445 bits (207), Expect = 7.252e-18
Identity = 57/199 (28.64%), Postives = 92/199 (46.23%), Query Frame = 1 
Query:  169 REARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFEST-QGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQK---NGNLIDYNHNT--TPNDLSMIKSLYNC 738
            R  R WP G + F    +F   +R + +Q +   +     CV F E T +  +I    +  GC + +G  G   Q +++      +  G + HE  H +GF H H R DRD  +SI   NIQ  Q +NF+K E   ++     Y+  S+MHY+ + F     ++T++ K   NG        T  +  D++  + LY C
Sbjct:  130 RPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKN--CDKFGIVVHELGHVIGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEVQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPSIGQRTRLSKGDIAQARKLYKC 324
[Close]
BLAST of tolloid like-1 vs. Ensembl Mouse
Match: Tll2 (tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044])

HSP 1 Score: 83.9593 bits (206), Expect = 8.005e-18
Identity = 54/199 (27.14%), Postives = 90/199 (45.23%), Query Frame = 1 
Query:  169 REARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFEST-QGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEG---CIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGN-----LIDYNHNTTPNDLSMIKSLYNC 738
            R  R WPGG + +    +F   +R + KQ +   +     CV F E T +   I    +  GC + +G  G   Q +++      +  G + HE  H +GF H H R DRD  ++I   NIQ  Q +NF+K E      +   Y+  S+MHY+ + F     ++T++ +  +      I      +  D++  + LY C
Sbjct:  151 RTERIWPGGVIPYVIGGNFTGTQRAIFKQAMRHWEKH--TCVTFVERTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKN--CDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRRDDNGVRPTIGQRVRLSQGDIAQARKLYKC 345
[Close]
BLAST of tolloid like-1 vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 83.5741 bits (205), Expect = 8.801e-18
Identity = 62/223 (27.80%), Postives = 97/223 (43.50%), Query Frame = 1 
Query:  118 LLSKADIAIINGFKHHQREARAWPGG-----KVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQN--------KGCYAMIGCTGERGQFLNLQNPWCM-NSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQ--KNGNLIDYNHNTTPNDLSMIKSLYNC 738
             L + DI   + F+        WP G     ++ F  +  +    R ++     E +     C++F        +A H Q          GC++ +G +G   Q ++L  P C+   +G + HE +H LGF H H R DRD +I +NW  I      NF+K     M   Y+  SVMHY   AF   +G  T+I    +   I    N + +D++ +  LYNC
Sbjct:   72 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRF--------VAYHGQRDFVSILPMAGCFSGVGRSGGM-QVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAF-SWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNC 281
[Close]
BLAST of tolloid like-1 vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 83.1889 bits (204), Expect = 1.043e-17
Identity = 62/223 (27.80%), Postives = 97/223 (43.50%), Query Frame = 1 
Query:  118 LLSKADIAIINGFKHHQREARAWPGG-----KVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQN--------KGCYAMIGCTGERGQFLNLQNPWCM-NSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQ--KNGNLIDYNHNTTPNDLSMIKSLYNC 738
             L + DI   + F+        WP G     ++ F  +  +    R ++     E +     C++F        +A H Q          GC++ +G +G   Q ++L  P C+   +G + HE +H LGF H H R DRD +I +NW  I      NF+K     M   Y+  SVMHY   AF   +G  T+I    +   I    N + +D++ +  LYNC
Sbjct:   51 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRF--------VAYHGQRDFVSILPMAGCFSGVGRSGGM-QVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAF-SWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNC 260
[Close]
BLAST of tolloid like-1 vs. UniProt/SwissProt
Match: sp|P07584|ASTA_ASTAS (Astacin OS=Astacus astacus OX=6715 PE=1 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 5.324e-21
Identity = 57/177 (32.20%), Postives = 92/177 (51.98%), Query Frame = 1 
Query:  184 WPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFF-ESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNF-VKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPND 708
            W GG + + FA    AD+  ++   + EL+     C++F   +T+  ++ +     GC++ +G      Q ++LQ   C+   GTI HE +HA+GF H H RMDRDN+++IN++N+  S   NF +      +  +Y+  S+MHY   +F  Q G+   I    N ID    T P D
Sbjct:   59 WSGGVIPYTFAGVSGADQSAILSG-MQELEEK--TCIRFVPRTTESDYVEIFTSGSGCWSYVGRI-SGAQQVSLQANGCV-YHGTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSMTSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNGIDL---TDPYD 227
[Close]
BLAST of tolloid like-1 vs. UniProt/SwissProt
Match: sp|Q19269|NAS14_CAEEL (Zinc metalloproteinase nas-14 OS=Caenorhabditis elegans OX=6239 GN=nas-14 PE=2 SV=2)

HSP 1 Score: 94.3597 bits (233), Expect = 1.249e-20
Identity = 62/194 (31.96%), Postives = 92/194 (47.42%), Query Frame = 1 
Query:  178 RAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNK---GCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGNL-IDYNHNTTPNDLSMIKSLYNC 738
            + WP G+V +        D+R  + Q  +E ++    CV+F   T      ++ +     GC + +G  G   Q ++L+   C  SKG I HE +HALGF H H R DRD+F+ IN  NI+     NF K    I+D     Y+  SVMHY   AF  + G  T+I K+    +   +  +  D   +  LY C
Sbjct:  123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKT--KTCVRFVPKTDDDFDYIYVKRNVAFGCSSYVGRAG-GNQTVSLEVDKCF-SKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFS-RNGKPTIIPKDNEADVGQRYKLSEMDSKKVNKLYQC 311
[Close]
BLAST of tolloid like-1 vs. UniProt/SwissProt
Match: sp|P55113|NAS7_CAEEL (Zinc metalloproteinase nas-7 OS=Caenorhabditis elegans OX=6239 GN=nas-7 PE=2 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.416e-20
Identity = 67/215 (31.16%), Postives = 102/215 (47.44%), Query Frame = 1 
Query:  127 KADIAIINGFKHH--QREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQG-PHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCE---GCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKN-----GNLIDYNHNTTPNDLSMIKSLYNC 738
            K+DI +    K +   R A+ WP  ++ +A +  +   +R L+ + + +       C++F     G P      +  GC++ +G T    Q L+L N  CM    TI HE +H +GF H H R DRDNFI I W+NI       F K +          Y+ +S++HY S AF  + G  TM+ K      GN  D++      D+S I  +YNC
Sbjct:   68 KSDIRLPRRHKRNGVSRAAKLWPNARIPYAISPHYSPHERALLAKAVKQYHE--KTCIRFVPRQTGEPDYLFIGKVDGCFSEVGRTSG-VQVLSLDN-GCMEY-ATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFS-KNGFPTMLPKVKSATIGNARDFSD----VDISKINRMYNC 272
[Close]
BLAST of tolloid like-1 vs. UniProt/SwissProt
Match: sp|P31580|HCE1_ORYLA (High choriolytic enzyme 1 OS=Oryzias latipes OX=8090 GN=hcea PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 6.324e-20
Identity = 54/153 (35.29%), Postives = 80/153 (52.29%), Query Frame = 1 
Query:  289 CVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQ-KNGNL-IDYNHNTTPNDLSMIKSLYNCR 741
            C++F   T            GCY+ +G  G + Q L++    CM S G I+HE  HALGF H   R DRD+++ INW NI  +  +NF K +   ++  Y+  S+MHY  DAF    G +++    N N+ I   +  +  D++ I  LYNCR
Sbjct:  120 CIRFVRRTNEYDFISVVSKTGCYSELGRKGGQ-QELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDITRINVLYNCR 270
[Close]
BLAST of tolloid like-1 vs. UniProt/SwissProt
Match: sp|P31581|HCE2_ORYLA (High choriolytic enzyme 2 OS=Oryzias latipes OX=8090 GN=hceb PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.788e-19
Identity = 57/154 (37.01%), Postives = 87/154 (56.49%), Query Frame = 1 
Query:  289 CVQFFEST-QGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQ-KNGNL-IDYNHNTTPNDLSMIKSLYNCR 741
            C++F   T +   I+V ++N GCY+ +G  G + Q L+L    CM S G I+HE  HALGF H   R DRD+++ INW+NI  +  +NF K +   ++  Y+  S+MHY  DAF    G +++    N N+ I   +  +  D++    LYNCR
Sbjct:  129 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGGQ-QELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDITRSNVLYNCR 279
[Close]
BLAST of tolloid like-1 vs. TrEMBL
Match: I1ZIC3 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast3 PE=2 SV=1)

HSP 1 Score: 499.59 bits (1285), Expect = 4.598e-178
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 1 
Query:   34 ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 741
            ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR
Sbjct:   17 ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 252
[Close]
BLAST of tolloid like-1 vs. TrEMBL
Match: A0A060Q786 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast6 PE=2 SV=1)

HSP 1 Score: 293.508 bits (750), Expect = 1.172e-96
Identity = 133/232 (57.33%), Postives = 169/232 (72.84%), Query Frame = 1 
Query:   43 VASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            V+ QCGC ++KL+ R  +++ +GDM+L K+DIA+INGFK H    + WP  +V F+FA  FP DK+ +V++CL ELQ DLGNCV+F EST   +I VH+ N+GCY+++G TG   Q LNLQNP CM SKGT+KHEFIHALGFMHTHMR DRDN I+I W  I +S C  FVKCEGC +DG YET SVMHY S  F C  G N + +++G LIDYNH T+ NDL M++  Y C
Sbjct:   17 VSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
[Close]
BLAST of tolloid like-1 vs. TrEMBL
Match: A0A060Q785 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast1 PE=2 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 2.500e-95
Identity = 131/233 (56.22%), Postives = 175/233 (75.11%), Query Frame = 1 
Query:   43 VASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIG-CTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            V+ QCGC ++KL+ R+ +++ +GDMLL K+DIA+INGFK+H    + WP   V ++FA +FP DK  +V+QCL ELQ DLGNCV+F ES QG +I V+++ +GCY+++G   G   Q LNL+NP CM SKGT+KHEFIHALGFMHTHMR DRDN I+INW  I++SQC  F KC+GC +DG YET SVMHY+S+ F C      +++K+G  I YNH+TTPNDL+MI++ Y C
Sbjct:   17 VSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 249
[Close]
BLAST of tolloid like-1 vs. TrEMBL
Match: A0A060Q714 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast2 PE=2 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 5.535e-90
Identity = 131/232 (56.47%), Postives = 162/232 (69.83%), Query Frame = 1 
Query:   52 QCGCNKK--KLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 741
            QCGC ++  KL TR  +N+ +GDMLL   DI IINGFK+H   ++ WP   V++ FA +FP DK   V+Q L ELQ DL NCV+F E   G +I V+++ +GCY+M+G TG   Q LNL+NP CM SKGTIKHEFIHALGFMHTHMR DRDN I+I W  I +SQC  F KC+GC  DG YET SVMHY+S+ F C      +++KNG  I YNH TT NDL MIK+ YNC+
Sbjct:   20 QCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNCK 251
[Close]
BLAST of tolloid like-1 vs. TrEMBL
Match: A0A060Q750 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast5 PE=2 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 5.000e-68
Identity = 110/234 (47.01%), Postives = 143/234 (61.11%), Query Frame = 1 
Query:   46 ASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERG--QFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 741
            A +CGC K KL  RA  +L + DMLL   D+  I GF+ + + AR W    + + F   FPADK+ +V++ +NEL +DL  CV++ EST G HI V +   GC++ +G   ++G  Q LNL+NP C+ SKGTIKHEFIHA GFMHT MR DRD F++I W  I S  C  FV C+ C     Y+T SVMHY S++F C     TM+ K    I YNH T   D  MIK LY C+
Sbjct:   21 AGKCGCQKSKL-KRAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 252
[Close]
BLAST of tolloid like-1 vs. Ensembl Cavefish
Match: hce2l2 (low choriolytic enzyme-like [Source:NCBI gene;Acc:103038533])

HSP 1 Score: 99.3673 bits (246), Expect = 3.340e-24
Identity = 59/187 (31.55%), Postives = 103/187 (55.08%), Query Frame = 1 
Query:  199 VSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFEST-QGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQK---NGNLIDYNHNTTPNDLSMIK--SLYNCR 741
            +++  +  +  + +  +KQ +  ++     CV++   T Q  H+ +  ++ GC++ +G  G R Q L+LQ P C+ S   + HE +HALGF+H   R DRDN+++I W NI   +  NF K +   MD  Y+ +S+MH+   A+  + G  T++QK   NG L     +  P++L ++K   LY C+
Sbjct:  117 IAYTLSPLYSEEDKMKIKQGMELIERK--TCVRYVPRTHQRDHLDIQPRS-GCWSYVGANGGR-QVLSLQTPECIGS-AVVAHELMHALGFLHEQSRADRDNYVTILWSNIWKDRTRNFAKFKTNNMDMPYDYKSIMHFGKYAY-SEDGNPTIVQKQNWNGKL---GQSFGPSELDILKINKLYQCK 294
[Close]
BLAST of tolloid like-1 vs. Ensembl Cavefish
Match: ENSAMXT00000033223.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 95.9005 bits (237), Expect = 3.561e-23
Identity = 60/184 (32.61%), Postives = 99/184 (53.80%), Query Frame = 1 
Query:  196 KVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTM--IQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            +V F  + DF +  R ++   +    S    C++F + ST+  ++++  ++ GCY+ +G TG + Q ++L    C+   G ++HE  HALGF H H R DRD +++INW+NI  +  +NF K     ++  Y+  SVMHY   AF    G+NT+  I      I    + +  D+  I +LYNC
Sbjct:   78 QVPFTVSADFSSTDRAVIAGAMATFHSK--TCIRFVDRSTEIDYLSIENRD-GCYSSLGRTGGK-QVVSLSTRGCV-YHGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFTI-NGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 255
[Close]
BLAST of tolloid like-1 vs. Ensembl Cavefish
Match: ENSAMXT00000056363.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 95.9005 bits (237), Expect = 3.561e-23
Identity = 60/184 (32.61%), Postives = 99/184 (53.80%), Query Frame = 1 
Query:  196 KVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTM--IQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            +V F  + DF +  R ++   +    S    C++F + ST+  ++++  ++ GCY+ +G TG + Q ++L    C+   G ++HE  HALGF H H R DRD +++INW+NI  +  +NF K     ++  Y+  SVMHY   AF    G+NT+  I      I    + +  D+  I +LYNC
Sbjct:   78 QVPFTVSADFSSTDRAVIAGAMATFHSK--TCIRFVDRSTEIDYLSIENRD-GCYSSLGRTGGK-QVVSLSTRGCV-YHGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFTI-NGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 255
[Close]
BLAST of tolloid like-1 vs. Ensembl Cavefish
Match: ENSAMXT00000044782.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 95.9005 bits (237), Expect = 3.561e-23
Identity = 60/184 (32.61%), Postives = 99/184 (53.80%), Query Frame = 1 
Query:  196 KVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTM--IQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            +V F  + DF +  R ++   +    S    C++F + ST+  ++++  ++ GCY+ +G TG + Q ++L    C+   G ++HE  HALGF H H R DRD +++INW+NI  +  +NF K     ++  Y+  SVMHY   AF    G+NT+  I      I    + +  D+  I +LYNC
Sbjct:   78 QVPFTVSADFSSTDRAVIAGAMATFHSK--TCIRFVDRSTEIDYLSIENRD-GCYSSLGRTGGK-QVVSLSTRGCV-YHGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFTI-NGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 255
[Close]
BLAST of tolloid like-1 vs. Ensembl Cavefish
Match: ENSAMXT00000035014.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 95.1301 bits (235), Expect = 5.329e-23
Identity = 60/184 (32.61%), Postives = 99/184 (53.80%), Query Frame = 1 
Query:  196 KVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTM--IQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            +V F  + DF +  R ++   +    S    C++F + ST+  ++++  ++ GCY+ +G TG + Q ++L    C+   G ++HE  HALGF H H R DRD +++INW+NI  +  +NF K     ++  Y+  SVMHY   AF    G+NT+  I      I    + +  D+  I +LYNC
Sbjct:   70 QVPFTVSADFSSTDRAVIAGAMATFHSK--TCIRFVDRSTEIDYLSIENRD-GCYSSLGRTGGK-QVVSLSTRGCV-YHGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTLYDYSSVMHYGKTAFTI-NGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 247
[Close]
BLAST of tolloid like-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007253.1 (pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1 gene:ENSPMAG00000006538.1 transcript:ENSPMAT00000007253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.0333 bits (201), Expect = 7.403e-18
Identity = 53/199 (26.63%), Postives = 94/199 (47.24%), Query Frame = 1 
Query:  169 REARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFEST-QGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGN-----LIDYNHNTTPNDLSMIKSLYNC 738
            R  R WPGG + +    +F   ++ + +Q +   +     CV F E T +  +I    +  GC + +G  G   Q +++      +  G + HE  H +GF H H R DRD+ ++I   NIQ  Q +NF+K E   +D    +Y+  S+MHY+ + F     ++T++ +  +      I      +  D++  + LY C
Sbjct:   17 RSERIWPGGVIPYIVGGNFTGSQKAMFRQAMRHWEKH--TCVTFIERTDEESYIVFTYRPCGCCSYVGRRGGGPQAISIGKN--CDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIKANIQPGQEYNFLKMEPGEVDSLGESYDFDSIMHYARNTFSRGIFLDTILPRPDDSGVRPAIGQRTRLSQGDIAQARKLYRC 211
[Close]
BLAST of tolloid like-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004913.1 (pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 gene:ENSPMAG00000004462.1 transcript:ENSPMAT00000004913.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.9138 bits (128), Expect = 2.741e-9
Identity = 32/95 (33.68%), Postives = 47/95 (49.47%), Query Frame = 1 
Query:  352 CYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG-NYETRSVMHYSSDAFGC 633
            C++ +G  G   Q +++         GT+ HE  HA+G++H   R DRD FIS+   N+Q  Q   F         G  Y+ +SVMHYS +   C
Sbjct:    1 CWSFVGMIGVGEQVVSIGRG--CEGVGTVAHELGHAVGYVHEQSRPDRDAFISVLEGNVQDGQLPQFAVYSATDPRGVPYDLKSVMHYSRNVRAC 93
[Close]
BLAST of tolloid like-1 vs. Ensembl Nematostella
Match: EDO34721 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNJ4])

HSP 1 Score: 95.1301 bits (235), Expect = 2.833e-23
Identity = 68/217 (31.34%), Postives = 105/217 (48.39%), Query Frame = 1 
Query:  109 GDMLLSKADIAIINGFKHHQREARA-----WPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMD---GNYETRSVMHYSSDAFGCQQGMNTMIQKNGNL-IDYNHNTTPNDLSMIKSLY 732
            GD+ L  +   I++      REA       WP G++ + F        +   +  ++E +++   C++F E T      +     GC++ IG +G + Q L+L   +C + KGT  HE +H LGF H   R DRDN+I+I+W NIQS + FNF K E   +D     Y+  S+MHY   AF    G  T++ K+    I      +  DL  +  LY
Sbjct:    4 GDIYLDSSTTRIMDQLMSPSREALVDPVSHWPNGRLPYKFGA-VSNSVKEAFRAAIDEYEAN--TCIRFVERTDETDYILVVSEGGCWSPIGRSGGQ-QKLSLGT-YC-DRKGTAMHELMHTLGFFHEQSRRDRDNYITIHWDNIQSDKQFNFQKYEHGKVDTLGAPYDYGSIMHYPMFAFS-SNGRATIVPKDPTASIGQRSGFSKVDLQQLNKLY 213
[Close]
BLAST of tolloid like-1 vs. Ensembl Nematostella
Match: EDO41420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4I5])

HSP 1 Score: 92.0485 bits (227), Expect = 1.804e-22
Identity = 65/229 (28.38%), Postives = 101/229 (44.10%), Query Frame = 1 
Query:   97 NLHVGDMLLSKAD-IAIINGFKHHQREARA------WPGGKVSFAFANDFPADKRNLVKQCLNELQSDL-----GNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            +L  GDM L+ A  +A   G       AR       WP G +++          R+L  + +N +++ +       C++F E T     A   + +GC +M+G TG R         W     GT+ HE  HALGF H   R DRDN+++I+W NI  +  FNF       +D     Y+ RSVMHY  +AF          +++G  +      +  D+  +  +Y C
Sbjct:    5 DLFEGDMRLTDAQRLAAQMGQDIDSPSARGGIKTGRWPEGVMAYTI-------DRSLSNRAMNAIRAGMKMWTDNTCIRFKERTNEYAYARFWRGRGCSSMVGRTGRRQDISLAGGCWY---PGTVAHEIGHALGFYHEQSRPDRDNYVTIHWNNISPNMKFNFNMYPRSTIDSLGTPYDYRSVMHYQGNAFSSNGRATITAKQSGVTLGNRRQLSKVDIQQMNLMYKC 223
[Close]
BLAST of tolloid like-1 vs. Ensembl Nematostella
Match: EDO30445 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0S0])

HSP 1 Score: 87.8113 bits (216), Expect = 1.355e-20
Identity = 63/213 (29.58%), Postives = 102/213 (47.89%), Query Frame = 1 
Query:  109 GDMLLSKADIAIIN-GFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKC---EGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYN 735
            GD+ L ++  AI+  G       AR W   +V + F +   ++ R++    + E ++    CV+F                GC++ IG +G   Q L+L        +GT  HE +HA+GF H   R+DRD++I+I W NI+    +NF K    E   +   Y+ +S+MHY   AF  +    T+I +NG  I    + +  D+  I +LYN
Sbjct:    5 GDIYLDRSTKAILGMGRDAMSSPARHWLNARVPYVFGS-VSSEVRSVFLAAIKEYEAK--TCVRFVPRRGERDYIYIVSEGGCFSSIGRSGGL-QKLSLGTG--CEHRGTAIHEMMHAIGFFHEQSRLDRDSYITIYWDNIKPGMEYNFRKYKRGEADTLGYAYDLKSIMHYPKYAF-TKNRQPTIIARNGANIGQRDSFSAIDIQQINALYN 210
[Close]
BLAST of tolloid like-1 vs. Ensembl Nematostella
Match: EDO42421 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1T1])

HSP 1 Score: 85.8853 bits (211), Expect = 1.839e-20
Identity = 59/193 (30.57%), Postives = 93/193 (48.19%), Query Frame = 1 
Query:  178 RAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLG--NCVQFFESTQGPHIAVHAQNKG-CYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            + WPGG V +     F    R + K  L +  SD     C++F + T   +  V+ +++G C + +G  G + Q+L+L +  C + KGTI HE  HALGF H   R DRD++++I + NI+     NF+K     +D     Y+  S+MHY    F        + +K G         +P D+  +  LY C
Sbjct:   10 KLWPGGVVVYELDPRFERSSRAMEK--LTQAISDYEKYTCIRFRKKTANDNDYVYIKDRGGCRSHVGRKGGK-QYLSLSS-GCWH-KGTIIHELGHALGFFHEQTRPDRDDYVTILFENIRLGMASNFMKHSAGEVDSLGTPYDYGSIMHYGPKIFSANGKPTIVAKKPGVKFGQRDGLSPIDIKQVNLLYKC 197
[Close]
BLAST of tolloid like-1 vs. Ensembl Nematostella
Match: EDO44568 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVF7])

HSP 1 Score: 87.8113 bits (216), Expect = 5.314e-20
Identity = 61/197 (30.96%), Postives = 92/197 (46.70%), Query Frame = 1 
Query:  178 RAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIG---CTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDG---NYETRSVMHYSSDAFGCQQGMNTM--IQKNG-NLIDYNHNTTPNDLSMIKSLYNCR 741
            R W    + + F + FP D R  + + + E       C++F E T   +       KGC++ +G      + GQ ++L +    N K  I HE +HA+GF H   R DR+ ++ + W NIQ  +  NF K     +D     Y+  S+MHY   +FG + G+ T+  I   G  L       T  DL  I +LYNCR
Sbjct:   42 RLWTNKIIPYQFESGFPNDLRPTILEAMEEYHKK--TCLRFKERTDERYFLRFVNKKGCWSSVGRSYWVEDVGQEVSLGS--GCNDKKIIMHELMHAIGFWHEQSRPDRNKYVEVLWENIQEGEAHNFNKYSHGEIDTLQVPYDFDSIMHYGRTSFG-KDGLETIRAIHDPGRELGRVIPAFTELDLREINALYNCR 233
[Close]
BLAST of tolloid like-1 vs. Ensembl Medaka
Match: mc6ast4 (astacin like metallo-protease [Source:NCBI gene;Acc:100049317])

HSP 1 Score: 102.449 bits (254), Expect = 1.583e-25
Identity = 69/220 (31.36%), Postives = 111/220 (50.45%), Query Frame = 1 
Query:  109 GDMLLSKADIAIINGFKHHQREARAWPGGK-------VSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFEST-QGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPN--DLSMIKSLYNC 738
            G   L + DIA+  G +     AR+    K       +++  + D+      L+K+ +  ++   G CV+F   T Q  ++ +  ++ GC++ +G  G R Q L+LQNP CM   G I HEF+HALGF+H   R DRDN+I++ W NI   +  NF K +   +D  Y+  S+MH+   A+    G  T++ KN   +     ++ +  D   I  LY C
Sbjct:   62 GTTTLREGDIAVSTGRRSKMCFARSCLWSKSVDGHVYIAYTISPDYTEADVKLIKKGMENIEE--GTCVRFVPQTHQRDYVDIQPKS-GCWSYLGARGGR-QTLSLQNPDCMQV-GVISHEFMHALGFVHEQSRFDRDNYITVMWPNIWRDRLRNFEKFKTDSLDLPYDYGSIMHFGMYAYS-MDGQPTLVAKNNRNMKLGQASSLSHTDKLKINRLYQC 275
[Close]
BLAST of tolloid like-1 vs. Ensembl Medaka
Match: mc6ast5 (astacin like metallo-protease [Source:NCBI gene;Acc:100049509])

HSP 1 Score: 97.4413 bits (241), Expect = 1.407e-23
Identity = 58/154 (37.66%), Postives = 78/154 (50.65%), Query Frame = 1 
Query:  283 GNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPN--DLSMIKSLYNC 738
            G C++F   T   H        GC++ +G TG   Q L+LQ P CM S G   HEF+HALGF+H   R DRD++++I W+NI      NF K     ++  Y+  SVMHY   AF    G  T+I +    I       P+  DL  I  LY+C
Sbjct:  129 GTCIKFVPRTHEAHFLDIQPRYGCWSFLGQTGG-SQTLSLQTPGCMWS-GIAAHEFMHALGFVHEQSRSDRDHYVTIVWKNIMPDHAHNFRKQATNNLNSPYDYSSVMHYGRYAFSGDGG-PTIIPRPDPYIPIGQRDGPSALDLHKINVLYDC 279
[Close]
BLAST of tolloid like-1 vs. Ensembl Medaka
Match: ENSORLT00000018637.2 (pep primary_assembly:ASM223467v1:3:32702055:32705063:-1 gene:ENSORLG00000014863.2 transcript:ENSORLT00000018637.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.4337 bits (228), Expect = 8.605e-22
Identity = 59/154 (38.31%), Postives = 88/154 (57.14%), Query Frame = 1 
Query:  289 CVQFFEST-QGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQ-KNGNL-IDYNHNTTPNDLSMIKSLYNCR 741
            C++F   T +   I+V ++N GCY+ +G  G + Q L+L    CM S G I+HE  HALGF H   R DRD+++ INW+NI  +  +NF K +   ++  Y+  S+MHY  DAF    G +T+    N N+ I   +  +  D++ I  LYNCR
Sbjct:  120 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGGQ-QELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDTITPIPNPNVPIGQRNGMSRWDITRINVLYNCR 270
[Close]
BLAST of tolloid like-1 vs. Ensembl Medaka
Match: hce (Oryzias latipes protease (hce), mRNA. [Source:RefSeq mRNA;Acc:NM_001201498])

HSP 1 Score: 88.9669 bits (219), Expect = 1.955e-20
Identity = 54/153 (35.29%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:  289 CVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQ-KNGNL-IDYNHNTTPNDLSMIKSLYNCR 741
            C++F   T            GCY+ +G  G   Q L++    CM S G I+HE  HALGF H   R DRD+++ INW NI  +  +NF K +   ++  Y+  S+MHY  DAF    G +++    N N+ I   +  +  D++ I  LYNCR
Sbjct:  129 CIRFVRRTNEYDFISVVSKTGCYSELGRKGGL-QELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDITRINVLYNCR 279
[Close]
BLAST of tolloid like-1 vs. Ensembl Medaka
Match: mnep (nephrosin [Source:NCBI gene;Acc:100049315])

HSP 1 Score: 86.6557 bits (213), Expect = 1.300e-19
Identity = 68/234 (29.06%), Postives = 112/234 (47.86%), Query Frame = 1 
Query:   76 LMTRATENL--HVGDMLLSKADIAIINGFKHHQR-EARA--WPGGK-----VSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFE-STQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNL-IDYNHNTTPNDLSMIKSLYNCR 741
            L+ +A  N+  ++ D L+   DIA+  G ++     AR   WP        V F  +  F + +R+ + Q L   +     C++F    +Q   + + ++  GC++ +G  G  GQ ++L    C+   GTI+HE +HALGF H   R DRD+ + I  +N+ S Q  NF K E   +   Y+  S+MHY   AF   +    +   + N+ I      + ND+  +  LY CR
Sbjct:   46 LLEKANVNIGKNLDDPLVMFGDIAVPTGLQNADPCTARGCLWPKSSDGNVYVPFRISGQFSSRERDTILQGLRSFEG--STCIRFTPHQSQRDFVDIQSRT-GCWSFVGRRGG-GQVVSLMRQGCV-FMGTIQHELLHALGFNHEQTRSDRDSHVRILLQNVISGQEHNFRKIETRNLGTPYDYNSIMHYGRFAFSRNREPTIVPIPDPNVPIGRATEMSSNDILRVNRLYECR 274
[Close]
BLAST of tolloid like-1 vs. Planmine SMEST
Match: SMESG000072804.1 (SMESG000072804.1)

HSP 1 Score: 499.59 bits (1285), Expect = 0.000e+0
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 1 
Query:   34 ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 741
            ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR
Sbjct:   31 ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 266
[Close]
BLAST of tolloid like-1 vs. Planmine SMEST
Match: SMESG000072804.1 (SMESG000072804.1)

HSP 1 Score: 499.59 bits (1285), Expect = 0.000e+0
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 1 
Query:   34 ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 741
            ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR
Sbjct:   17 ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 252
[Close]
BLAST of tolloid like-1 vs. Planmine SMEST
Match: SMESG000072804.1 (SMESG000072804.1)

HSP 1 Score: 499.204 bits (1284), Expect = 0.000e+0
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 1 
Query:   34 ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 741
            ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR
Sbjct:   13 ANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 248
[Close]
BLAST of tolloid like-1 vs. Planmine SMEST
Match: SMESG000072806.1 (SMESG000072806.1)

HSP 1 Score: 421.394 bits (1082), Expect = 1.013e-150
Identity = 196/234 (83.76%), Postives = 211/234 (90.17%), Query Frame = 1 
Query:   37 NEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            NEVASQCGC+KKKL+TRAT  L VGDM+LSK DIAIINGFKHHQ +AR WPGG V FAF NDFP DKRNLVKQCL ELQSDLGNCV+FFESTQGP+I VHAQ++GCYAMIG TGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMR DRD+FISINW+NIQS QCFNF KC GCI+DG Y+TRSVMHYSSDAFGCQQG+ TMIQ NG LIDYNHNTTPNDL MI++LY C
Sbjct:   14 NEVASQCGCSKKKLLTRATNGLRVGDMVLSKGDIAIINGFKHHQMDARIWPGGNVLFAFGNDFPYDKRNLVKQCLYELQSDLGNCVRFFESTQGPYIEVHAQDRGCYAMIGFTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRKDRDDFISINWQNIQSDQCFNFEKCNGCILDGEYDTRSVMHYSSDAFGCQQGLYTMIQTNGELIDYNHNTTPNDLDMIRNLYRC 247
[Close]
BLAST of tolloid like-1 vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 293.508 bits (750), Expect = 3.260e-100
Identity = 133/232 (57.33%), Postives = 169/232 (72.84%), Query Frame = 1 
Query:   43 VASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 738
            V+ QCGC ++KL+ R  +++ +GDM+L K+DIA+INGFK H    + WP  +V F+FA  FP DK+ +V++CL ELQ DLGNCV+F EST   +I VH+ N+GCY+++G TG   Q LNLQNP CM SKGT+KHEFIHALGFMHTHMR DRDN I+I W  I +S C  FVKCEGC +DG YET SVMHY S  F C  G N + +++G LIDYNH T+ NDL M++  Y C
Sbjct:   17 VSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
The following BLAST results are available for this feature:
BLAST of tolloid like-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ASTL2.401e-1927.44astacin like metalloendopeptidase [Source:HGNC Sym... [more]
TLL13.215e-1927.64tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
BMP11.800e-1828.64bone morphogenetic protein 1 [Source:HGNC Symbol;A... [more]
TLL12.001e-1827.64tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
TLL12.010e-1827.64tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
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BLAST of tolloid like-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nas-149.820e-2231.96Zinc metalloproteinase nas-14 [Source:UniProtKB/S... [more]
nas-71.113e-2131.16Zinc metalloproteinase nas-7 [Source:UniProtKB/Sw... [more]
nas-47.169e-2028.72Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-279.460e-2029.54Zinc metalloproteinase nas-27 [Source:UniProtKB/S... [more]
nas-42.100e-1928.72Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
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BLAST of tolloid like-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG67631.877e-3031.98gene:FBgn0039069 transcript:FBtr0084378[more]
CG67631.877e-3031.98gene:FBgn0039069 transcript:FBtr0334497[more]
CG57156.929e-2229.91gene:FBgn0039180 transcript:FBtr0084634[more]
CG118653.344e-2130.69gene:FBgn0028947 transcript:FBtr0080753[more]
CG152551.052e-1929.44gene:FBgn0028950 transcript:FBtr0080785[more]
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BLAST of tolloid like-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l11.251e-2238.56high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:... [more]
he1.22.062e-2229.55hatching enzyme 1, tandem dupliate 2 [Source:ZFIN;... [more]
hce2l13.074e-2238.56high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:... [more]
he1.14.502e-2229.55hatching enzyme 1, tandem duplicate 1 [Source:ZFIN... [more]
he1.14.502e-2229.55hatching enzyme 1, tandem duplicate 1 [Source:ZFIN... [more]
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BLAST of tolloid like-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
golt1b6.330e-2332.17golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
kpna43.053e-2230.77karyopherin alpha 4 (importin alpha 3) [Source:Xen... [more]
polr2l.26.495e-2230.37polymerase (RNA) II (DNA directed) polypeptide L, ... [more]
ENSXETT00000022029.17.774e-2029.90astacin-like metalloendopeptidase [Source:NCBI gen... [more]
golt1b9.554e-2036.18golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
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BLAST of tolloid like-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tll11.367e-1827.64tolloid-like [Source:MGI Symbol;Acc:MGI:106923][more]
Bmp17.252e-1828.64bone morphogenetic protein 1 [Source:MGI Symbol;Ac... [more]
Tll28.005e-1827.14tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044][more]
Astl8.801e-1827.80astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl1.043e-1727.80astacin-like metalloendopeptidase (M12 family) [So... [more]
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BLAST of tolloid like-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P07584|ASTA_ASTAS5.324e-2132.20Astacin OS=Astacus astacus OX=6715 PE=1 SV=2[more]
sp|Q19269|NAS14_CAEEL1.249e-2031.96Zinc metalloproteinase nas-14 OS=Caenorhabditis el... [more]
sp|P55113|NAS7_CAEEL1.416e-2031.16Zinc metalloproteinase nas-7 OS=Caenorhabditis ele... [more]
sp|P31580|HCE1_ORYLA6.324e-2035.29High choriolytic enzyme 1 OS=Oryzias latipes OX=80... [more]
sp|P31581|HCE2_ORYLA1.788e-1937.01High choriolytic enzyme 2 OS=Oryzias latipes OX=80... [more]
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BLAST of tolloid like-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZIC34.598e-178100.00Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7861.172e-9657.33Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7852.500e-9556.22Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7145.535e-9056.47Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7505.000e-6847.01Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
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BLAST of tolloid like-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l23.340e-2431.55low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
ENSAMXT00000033223.13.561e-2332.61high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
ENSAMXT00000056363.13.561e-2332.61high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
ENSAMXT00000044782.13.561e-2332.61high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
ENSAMXT00000035014.15.329e-2332.61high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
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BLAST of tolloid like-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000007253.17.403e-1826.63pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1... [more]
ENSPMAT00000004913.12.741e-933.68pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 g... [more]
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BLAST of tolloid like-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tolloid like-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO347212.833e-2331.34Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414201.804e-2228.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO304451.355e-2029.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO424211.839e-2030.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO445685.314e-2030.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of tolloid like-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mc6ast41.583e-2531.36astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mc6ast51.407e-2337.66astacin like metallo-protease [Source:NCBI gene;Ac... [more]
ENSORLT00000018637.28.605e-2238.31pep primary_assembly:ASM223467v1:3:32702055:327050... [more]
hce1.955e-2035.29Oryzias latipes protease (hce), mRNA. [Source:RefS... [more]
mnep1.300e-1929.06nephrosin [Source:NCBI gene;Acc:100049315][more]
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BLAST of tolloid like-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000072804.10.000e+0100.00SMESG000072804.1[more]
SMESG000072804.10.000e+0100.00SMESG000072804.1[more]
SMESG000072804.10.000e+0100.00SMESG000072804.1[more]
SMESG000072806.11.013e-15083.76SMESG000072806.1[more]
SMESG000072791.13.260e-10057.33SMESG000072791.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30010228
GenBankAFJ24777.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010228 ID=SMED30010228|Name=tolloid like-1|organism=Schmidtea mediterranea sexual|type=transcript|length=814bp
ATCTAGTTTCTTATCCTTATCGTATTAGTGCTTGCCAACGAGGTGGCCAG
CCAATGTGGATGTAACAAGAAGAAATTAATGACTCGAGCTACGGAAAACC
TTCACGTGGGAGACATGCTGCTAAGCAAGGCGGATATCGCTATTATCAAC
GGGTTTAAGCACCATCAAAGAGAAGCTAGAGCATGGCCTGGAGGAAAAGT
TTCATTTGCATTTGCAAATGATTTCCCTGCCGATAAAAGAAATCTGGTCA
AGCAATGTTTGAATGAACTGCAATCTGACTTGGGTAACTGTGTCCAATTC
TTTGAATCTACTCAAGGTCCGCACATTGCAGTTCATGCTCAAAACAAAGG
TTGCTATGCAATGATTGGATGTACTGGTGAAAGAGGTCAATTTTTGAATC
TACAAAATCCGTGGTGCATGAATTCAAAAGGAACAATCAAGCATGAGTTT
ATCCACGCTCTCGGTTTCATGCATACTCACATGAGGATGGACAGGGACAA
CTTTATTTCTATTAACTGGAGAAATATTCAATCAAGCCAATGTTTTAACT
TTGTGAAATGCGAAGGTTGCATTATGGACGGAAATTACGAAACCAGAAGT
GTGATGCACTATAGCAGTGACGCCTTCGGATGCCAACAAGGAATGAATAC
CATGATCCAAAAGAACGGAAATTTGATAGATTATAACCATAACACTACTC
CCAATGACTTAAGTATGATCAAGAGTCTCTATAATTGCCGATAAGTTTAC
GAAATAAATATTAAGCTATCCCTTTATTTTTATGTCTTTTTACTTATGTC
CCTCATTATTAAAA
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protein sequence of SMED30010228-orf-1

>SMED30010228-orf-1 ID=SMED30010228-orf-1|Name=SMED30010228-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=222bp
MTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFP
ADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTG
ERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNI
QSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLI
DYNHNTTPNDLSMIKSLYNCR*
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Aliases
The feature 'tolloid like-1' has the following synonyms
Synonym
tolloid-like-1
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR001506Peptidase_M12A
IPR006026Peptidase_Metallo
IPR024079MetalloPept_cat_dom_sf
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
GO:0008233peptidase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001506Peptidase M12APRINTSPR00480ASTACINcoord: 193..208
score: 42.81
coord: 138..156
score: 45.7
coord: 157..174
score: 45.56
coord: 230..243
score: 26.79
IPR001506Peptidase M12APFAMPF01400Astacincoord: 59..244
e-value: 3.2E-38
score: 131.3
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 56..196
e-value: 1.3E-18
score: 77.9
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3DG3DSA:3.40.390.10coord: 10..245
e-value: 4.5E-47
score: 162.5
NoneNo IPR availablePANTHERPTHR10127DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAININGcoord: 29..244
NoneNo IPR availablePROSITEPS51864ASTACINcoord: 45..245
score: 37.043
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..15
score: 0.749
NoneNo IPR availableSUPERFAMILYSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 58..244