SMED30010227

Overview
NameSMED30010227
Smed IDSMED30010227
Length (bp)1557
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010227 (SMED30010227) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010227 (SMED30010227) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010227 (SMED30010227) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010227 (SMED30010227) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010227

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30010227SMESG000078872.1 SmedASXL_015709SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30010227SMESG000078872.1 dd_Smed_v4_673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30010227SMESG000078872.1 dd_Smed_v4_673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010227SMESG000078872.1 dd_Smed_v4_673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010227SMESG000078872.1 dd_Smed_v4_673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010227SMESG000078872.1 dd_Smed_v4_673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010227SMESG000078872.1 dd_Smed_v4_673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30010227SMESG000078872.1 dd_Smed_v4_673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30010227SMESG000078872.1 dd_Smed_v4_673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010227SMESG000078872.1 Contig48311uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010227SMESG000078872.1 Contig48311newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neoblastSMED30010227SMESG000078872.1 dd_Smed_v6_673_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010227 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000929:76431..78077 +7589
Homology
[Close]
BLAST of SMED30010227 vs. TrEMBL
Match: A0A267EMV5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008359g2 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 4.795e-12
Identity = 38/88 (43.18%), Postives = 57/88 (64.77%), Query Frame = 2 
Query: 1043 SKLSGRKGYTAYHFVADKE-INSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPEL 1303
            S LS R+G+TA+    + E +  KGYLF +L+R +D E VEK++ V  +KD    +FDLPS +++  +  W DG+YD +  L E+PEL
Sbjct:  521 SLLSAREGFTAFVLHTEAETVRGKGYLFGALRRCLDPEQVEKVQYVTFSKDRKSLLFDLPSNMEDIVEKRWTDGRYDRLEKLTELPEL 608
[Close]
BLAST of SMED30010227 vs. TrEMBL
Match: A0A1I8HWN6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 5.043e-12
Identity = 38/88 (43.18%), Postives = 57/88 (64.77%), Query Frame = 2 
Query: 1043 SKLSGRKGYTAYHFVADKE-INSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPEL 1303
            S LS R+G+TA+    + E +  KGYLF +L+R +D E VEK++ V  +KD    +FDLPS +++  +  W DG+YD +  L E+PEL
Sbjct:  519 SLLSAREGFTAFVLHTEAETVRGKGYLFGALRRCLDPEQVEKVQYVTFSKDRKSLLFDLPSNMEDIVEKRWTDGRYDRLEKLTELPEL 606
[Close]
BLAST of SMED30010227 vs. TrEMBL
Match: A0A267EN73 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008359g1 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 5.055e-12
Identity = 38/88 (43.18%), Postives = 57/88 (64.77%), Query Frame = 2 
Query: 1043 SKLSGRKGYTAYHFVADKE-INSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPEL 1303
            S LS R+G+TA+    + E +  KGYLF +L+R +D E VEK++ V  +KD    +FDLPS +++  +  W DG+YD +  L E+PEL
Sbjct:  521 SLLSAREGFTAFVLHTEAETVRGKGYLFGALRRCLDPEQVEKVQYVTFSKDRKSLLFDLPSNMEDIVEKRWTDGRYDRLEKLTELPEL 608
[Close]
BLAST of SMED30010227 vs. TrEMBL
Match: A0A1I8IRT2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 5.078e-12
Identity = 38/88 (43.18%), Postives = 57/88 (64.77%), Query Frame = 2 
Query: 1043 SKLSGRKGYTAYHFVADKE-INSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPEL 1303
            S LS R+G+TA+    + E +  KGYLF +L+R +D E VEK++ V  +KD    +FDLPS +++  +  W DG+YD +  L E+PEL
Sbjct:  519 SLLSAREGFTAFVLHTEAETVRGKGYLFGALRRCLDPEQVEKVQYVTFSKDRKSLLFDLPSNMEDIVEKRWTDGRYDRLEKLTELPEL 606
[Close]
BLAST of SMED30010227 vs. TrEMBL
Match: A0A267H216 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008359g3 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 5.105e-12
Identity = 38/88 (43.18%), Postives = 57/88 (64.77%), Query Frame = 2 
Query: 1043 SKLSGRKGYTAYHFVADKE-INSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPEL 1303
            S LS R+G+TA+    + E +  KGYLF +L+R +D E VEK++ V  +KD    +FDLPS +++  +  W DG+YD +  L E+PEL
Sbjct:  522 SLLSAREGFTAFVLHTEAETVRGKGYLFGALRRCLDPEQVEKVQYVTFSKDRKSLLFDLPSNMEDIVEKRWTDGRYDRLEKLTELPEL 609
[Close]
BLAST of SMED30010227 vs. Ensembl Cavefish
Match: ddx21 (DExD-box helicase 21 [Source:NCBI gene;Acc:103032160])

HSP 1 Score: 56.225 bits (134), Expect = 6.297e-8
Identity = 30/92 (32.61%), Postives = 50/92 (54.35%), Query Frame = 2 
Query: 1028 TFESGSKLSGRKGYTAYHFVADKEINSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPEL 1303
            T E  S L+   G+T        E+N+ G  +++LK  + EE  ++I R+   KD MG  FD+P++  +  Q  W DG+   +++  E+PEL
Sbjct:  609 TLEQRSLLNSDAGFTTMIMNCSVEMNNIGTAWRALKEQLGEEIDDQIRRMIFLKDQMGVCFDVPADKVKEIQDKWEDGRRWQLTVATELPEL 700
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BLAST of SMED30010227 vs. Ensembl Medaka
Match: ddx21 (DExD-box helicase 21 [Source:NCBI gene;Acc:101155411])

HSP 1 Score: 50.0618 bits (118), Expect = 5.156e-6
Identity = 41/174 (23.56%), Postives = 82/174 (47.13%), Query Frame = 2 
Query:   77 VISTLDLSQEILNKLKDKKVESLNQVETKVFNAINNRTXXXXXXXXXXXXXXXXXIKSLESVKK----LKLTRLARLIVLCDSSELCIKIGEEIKSLITKELVIILCQELVKLKSNKKSFEGAVDIVIGTPKRLNELILSETLKTKAIKNIIVLNFDK-LDVHLVEKF--VLSL 577
              S   +SQ  +NKLK + V  L  ++ K FN + +   V   +   +    +  I  +E ++K        R  +++VL  + EL I++ ++ K  ++K+L I              +    +DI++GTP R+ + + +  L    +K++++   D+ LD+   E+   +LSL
Sbjct:   58 AFSNFRISQVTINKLKARGVSYLFDIQVKTFNPVYDGEDVLAQARTGTGKTFSFAIPLVERLQKEGGETTRGRPPKVLVLTPTRELAIQVAKDFKD-VSKKLAIACFYGGSSYNPQLDAIRNGIDILVGTPGRIKDHLQNHKLNLSDLKHVVLDEVDQMLDMGFAEQVEEILSL 230
[Close]
BLAST of SMED30010227 vs. Planmine SMEST
Match: SMESG000078872.1 (SMESG000078872.1)

HSP 1 Score: 724.546 bits (1869), Expect = 0.000e+0
Identity = 474/482 (98.34%), Postives = 479/482 (99.38%), Query Frame = 2 
Query:   71 MTVISTLDLSQEILNKLKDKKVESLNQVETKVFNAINNRTXXXXXXXXXXXXXXXXXIKSLESVKKLKLTRLARLIVLCDSSELCIKIGEEIKSLITKELVIILCQELVKLKSNKKSFEGAVDIVIGTPKRLNELILSETLKTKAIKNIIVLNFDKLDVHLVEKFVLSLPKVSVDSEKSIQYVVTSSDLGEKVKSFIGKHTNENFENIEVESEDXXXXXXXXXXXXXXXXXXXXLNLSNNCEKNEDKESETEVNDTKLEEMDEDLLNKIRSAEDFIKFHSMSKLIIEEFSDIEGXXXXXXXXXXKKHTKDIKPVGVKPKTFESGSKLSGRKGYTAYHFVADKEINSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPELSTEDRSVSTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYKRHGSIEGGSSAKLQKFD 1516
            MTVISTLDLSQEILNKLKDKKVESLNQVETKVFNAINNRTSVSIPSSVASVAFIAPFIKSLESVKKLKLTRLARLIVLCDSSELCIKIGEEIKSLITKELVIILCQELVKLKSNKKSFEGAVDIVIGTPKRLNELILSETLKTKAIKNIIVLNFDKLDV LVEKFVLSLPKVSVDSEKSIQYVVTSSDLGEKVKSFIGKHTN+NFENIEVESEDEKSTSENESVSSDDDVKKNVLNLSNN EK+EDKESETEVNDTKLEEMDEDLLNKIRSAEDFIKFHSMSKLII+EFSDIEGLSRLISLLSSK+HTKDIKPVGVKPKTFESGSKLSGR+GYTAYHFVADKEINSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPELSTEDRSVSTRGR FERGGFRGGRGNDRGGFRGGRGNDRGGRGGRGRGNDRGGYKRHGSIEGGSSAKLQKFD
Sbjct:    1 MTVISTLDLSQEILNKLKDKKVESLNQVETKVFNAINNRTSVSIPSSVASVAFIAPFIKSLESVKKLKLTRLARLIVLCDSSELCIKIGEEIKSLITKELVIILCQELVKLKSNKKSFEGAVDIVIGTPKRLNELILSETLKTKAIKNIIVLNFDKLDVDLVEKFVLSLPKVSVDSEKSIQYVVTSSDLGEKVKSFIGKHTNDNFENIEVESEDEKSTSENESVSSDDDVKKNVLNLSNNSEKDEDKESETEVNDTKLEEMDEDLLNKIRSAEDFIKFHSMSKLIIQEFSDIEGLSRLISLLSSKRHTKDIKPVGVKPKTFESGSKLSGREGYTAYHFVADKEINSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPELSTEDRSVSTRGRSFERGGFRGGRGNDRGGFRGGRGNDRGGRGGRGRGNDRGGYKRHGSIEGGSSAKLQKFD 482
[Close]
BLAST of SMED30010227 vs. Planmine SMEST
Match: SMESG000065959.1 (SMESG000065959.1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.615e-14
Identity = 38/92 (41.30%), Postives = 56/92 (60.87%), Query Frame = 2 
Query: 1043 SKLSGRKGYTAYHFVADKEINSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPEL-STED 1315
            S LS  +G+T Y      EI  KGY + +LK+ + +  VE+++ +Q  K   G VFD+P+E DE  + SW DGKY+ +  + +VPEL  TED
Sbjct:  369 SLLSSAEGFTTYLLTTTDEIKFKGYAYSALKKCLPDNIVEQVKLLQFVKGRKGLVFDMPAEHDEIVKTSWTDGKYNTLVKVVDVPELEETED 460
[Close]
BLAST of SMED30010227 vs. Planmine SMEST
Match: SMESG000065959.1 (SMESG000065959.1)

HSP 1 Score: 75.485 bits (184), Expect = 4.167e-14
Identity = 38/92 (41.30%), Postives = 56/92 (60.87%), Query Frame = 2 
Query: 1043 SKLSGRKGYTAYHFVADKEINSKGYLFKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYDVMSLLEEVPEL-STED 1315
            S LS  +G+T Y      EI  KGY + +LK+ + +  VE+++ +Q  K   G VFD+P+E DE  + SW DGKY+ +  + +VPEL  TED
Sbjct:  496 SLLSSAEGFTTYLLTTTDEIKFKGYAYSALKKCLPDNIVEQVKLLQFVKGRKGLVFDMPAEHDEIVKTSWTDGKYNTLVKVVDVPELEETED 587
The following BLAST results are available for this feature:
BLAST of SMED30010227 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267EMV54.795e-1243.18Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8HWN65.043e-1243.18Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267EN735.055e-1243.18Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8IRT25.078e-1243.18Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267H2165.105e-1243.18Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of SMED30010227 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ddx216.297e-832.61DExD-box helicase 21 [Source:NCBI gene;Acc:1030321... [more]
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BLAST of SMED30010227 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010227 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ddx215.156e-623.56DExD-box helicase 21 [Source:NCBI gene;Acc:1011554... [more]
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BLAST of SMED30010227 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000078872.10.000e+098.34SMESG000078872.1[more]
SMESG000065959.13.615e-1441.30SMESG000065959.1[more]
SMESG000065959.14.167e-1441.30SMESG000065959.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010227 ID=SMED30010227|Name=SMED30010227|organism=Schmidtea mediterranea sexual|type=transcript|length=1557bp
TGGGAATTGTGATGTAAATTGTTTGAAATAGTACTTTTATTTAAAAATAT
ATTTAAAAACGTTTTTCAAAATGACCGTTATTAGTACTTTAGATTTATCT
CAAGAAATCCTAAATAAACTAAAAGATAAGAAAGTTGAAAGTCTGAATCA
AGTGGAAACCAAAGTTTTTAATGCTATTAACAACCGAACAAGTGTTTCTA
TACCCAGTTCTGTTGCTTCTGTCGCCTTTATCGCCCCATTTATCAAAAGC
TTGGAGTCAGTTAAAAAATTAAAACTAACCCGATTAGCTAGACTAATTGT
TCTATGTGATTCCAGTGAGTTGTGTATCAAAATAGGAGAAGAAATTAAAA
GTTTGATAACGAAAGAATTAGTAATTATACTCTGCCAAGAACTAGTGAAA
CTCAAATCAAACAAAAAGAGTTTCGAAGGAGCCGTTGATATTGTTATTGG
AACTCCAAAAAGATTAAACGAATTAATTTTATCTGAAACATTGAAAACGA
AAGCCATCAAAAATATCATCGTCTTAAATTTTGATAAATTAGATGTTCAT
TTGGTTGAAAAATTCGTTTTGAGCTTACCTAAAGTTTCAGTAGATTCTGA
AAAATCGATACAATATGTTGTCACATCATCCGACCTAGGTGAAAAAGTTA
AATCATTTATTGGAAAACATACAAACGAGAATTTTGAAAACATTGAAGTT
GAAAGTGAGGATGAAAAAAGTACTAGTGAAAATGAAAGTGTAAGCAGTGA
CGACGATGTAAAGAAGAACGTTTTAAACCTTTCTAACAATTGTGAAAAAA
ATGAGGACAAAGAAAGCGAAACCGAAGTAAATGATACTAAATTGGAAGAG
ATGGATGAAGATCTACTTAACAAAATAAGGTCCGCCGAAGATTTTATTAA
GTTTCATTCCATGTCTAAGTTAATTATTGAAGAATTTTCTGACATAGAAG
GATTATCGAGGTTAATAAGCTTGTTATCCTCTAAAAAACATACTAAAGAT
ATTAAACCTGTCGGAGTTAAACCAAAAACATTTGAAAGTGGATCGAAATT
AAGTGGACGAAAAGGTTATACAGCTTATCACTTTGTGGCCGACAAGGAAA
TAAACAGCAAAGGTTATCTCTTCAAATCTTTGAAAAGGTTAATTGACGAA
GAAGCTGTTGAGAAAATTGAACGTGTTCAGCATACAAAAGATTTAATGGG
ATTTGTCTTTGATTTACCTTCTGAACTTGATGAAACATTTCAAGGAAGTT
GGAGAGATGGTAAATACGATGTAATGTCACTGTTAGAAGAAGTTCCAGAA
TTGTCTACAGAGGATAGAAGTGTATCAACTCGGGGTAGGGGTTTTGAACG
AGGTGGATTCCGTGGAGGAAGAGGAAACGACAGAGGTGGCTTCCGTGGTG
GTAGAGGAAATGATAGAGGTGGTCGTGGAGGTAGAGGTCGTGGTAATGAT
CGAGGTGGTTATAAAAGACATGGATCGATTGAGGGAGGTTCTTCTGCAAA
ACTTCAAAAGTTTGATTAAAACTTTATGTAAATAAAATGTGCAATATACT
TGGTTTT
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protein sequence of SMED30010227-orf-1

>SMED30010227-orf-1 ID=SMED30010227-orf-1|Name=SMED30010227-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=483bp
MTVISTLDLSQEILNKLKDKKVESLNQVETKVFNAINNRTSVSIPSSVAS
VAFIAPFIKSLESVKKLKLTRLARLIVLCDSSELCIKIGEEIKSLITKEL
VIILCQELVKLKSNKKSFEGAVDIVIGTPKRLNELILSETLKTKAIKNII
VLNFDKLDVHLVEKFVLSLPKVSVDSEKSIQYVVTSSDLGEKVKSFIGKH
TNENFENIEVESEDEKSTSENESVSSDDDVKKNVLNLSNNCEKNEDKESE
TEVNDTKLEEMDEDLLNKIRSAEDFIKFHSMSKLIIEEFSDIEGLSRLIS
LLSSKKHTKDIKPVGVKPKTFESGSKLSGRKGYTAYHFVADKEINSKGYL
FKSLKRLIDEEAVEKIERVQHTKDLMGFVFDLPSELDETFQGSWRDGKYD
VMSLLEEVPELSTEDRSVSTRGRGFERGGFRGGRGNDRGGFRGGRGNDRG
GRGGRGRGNDRGGYKRHGSIEGGSSAKLQKFD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR035979RBD_domain_sf
IPR011545DEAD/DEAH_box_helicase_dom
IPR027417P-loop_NTPase
IPR012562GUCT
IPR012677Nucleotide-bd_a/b_plait_sf
IPR006928htUSP
Vocabulary: molecular function
TermDefinition
GO:0004386helicase activity
GO:0003723RNA binding
GO:0000166nucleotide binding
GO:0008233peptidase activity
GO:0003676nucleic acid binding
GO:0005524ATP binding
GO:0016787hydrolase activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012562GUCTPFAMPF08152GUCTcoord: 322..411
e-value: 8.2E-18
score: 64.5
NoneNo IPR availableGENE3DG3DSA:3.30.70.2280coord: 330..407
e-value: 2.2E-14
score: 55.1
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 4..206
e-value: 3.5E-16
score: 61.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 410..433
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 207..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 410..482
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 441..467
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 207..229
NoneNo IPR availableCDDcd12937GUCT_RH7_likecoord: 325..409
e-value: 5.37086E-22
score: 88.0075
NoneNo IPR availableCDDcd00268DEADccoord: 52..203
e-value: 6.09613E-11
score: 59.3777
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 51..187
e-value: 1.5E-7
score: 31.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 5..203
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 325..399