Keratinocyte associated protein 2

Overview
NameKeratinocyte associated protein 2
Smed IDSMED30010216
Length (bp)489
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Keratinocyte associated protein 2 (SMED30010216) t-SNE clustered cells

Violin plots show distribution of expression levels for Keratinocyte associated protein 2 (SMED30010216) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Keratinocyte associated protein 2 (SMED30010216) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Keratinocyte associated protein 2 (SMED30010216) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30010216

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30010216h1SMcG0006367 dd_Smed_v4_1305_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30010216h1SMcG0006367 dd_Smed_v4_1305_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30010216h1SMcG0006367 dd_Smed_v4_1305_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010216h1SMcG0006367 dd_Smed_v4_1305_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Human
Match: KRTCAP2 (keratinocyte associated protein 2 [Source:HGNC Symbol;Acc:HGNC:28942])

HSP 1 Score: 81.2629 bits (199), Expect = 1.979e-19
Identity = 45/97 (46.39%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  148 FATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            FA +Q++   + +++ LTI GG + S +F+  LT FNN E ++FGKGFQ++IFPE+++ L+L+  A+G++HR+C+T C  FS+ GLYY+NK+S   Y
Sbjct:   46 FAGMQMYSRQLASTEWLTIQGGLLGSGLFVFSLTAFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISSTLY 142
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Fly
Match: CG31460 (gene:FBgn0051460 transcript:FBtr0081873)

HSP 1 Score: 78.1814 bits (191), Expect = 8.886e-19
Identity = 45/109 (41.28%), Postives = 77/109 (70.64%), Query Frame = 1 
Query:  118 ILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNV 444
            I++  LS+++F TL+    +   S+L  + GG++ S++FIL LT  +N E ++FG+ FQ+++ PE++  L L+  AAG+VHR+C T  + FS+ GLY+LN++S K+Y+V
Sbjct:   15 IISGLLSIVLFGTLRFCSEWFNDSQLRVLLGGYLFSWVFILSLTCVSNAEMVVFGQDFQAKLLPEIIFCLSLTVAAAGLVHRVCATTSVLFSLVGLYFLNRISTKYYSV 123
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Fly
Match: CG31460 (gene:FBgn0051460 transcript:FBtr0339327)

HSP 1 Score: 78.1814 bits (191), Expect = 8.886e-19
Identity = 45/109 (41.28%), Postives = 77/109 (70.64%), Query Frame = 1 
Query:  118 ILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNV 444
            I++  LS+++F TL+    +   S+L  + GG++ S++FIL LT  +N E ++FG+ FQ+++ PE++  L L+  AAG+VHR+C T  + FS+ GLY+LN++S K+Y+V
Sbjct:   15 IISGLLSIVLFGTLRFCSEWFNDSQLRVLLGGYLFSWVFILSLTCVSNAEMVVFGQDFQAKLLPEIIFCLSLTVAAAGLVHRVCATTSVLFSLVGLYFLNRISTKYYSV 123
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Zebrafish
Match: krtcap2 (keratinocyte associated protein 2 [Source:ZFIN;Acc:ZDB-GENE-060825-91])

HSP 1 Score: 87.0409 bits (214), Expect = 3.287e-22
Identity = 51/97 (52.58%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  148 FATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            FA +Q+F   + +S+ LTI GGF+ S +FI  LT FNN E ++FGKGFQ++IFPE+V+ + L+  A+G+VHR+C+T CL FSV  LYY+NK+S   Y
Sbjct:   20 FAGMQMFSRQLASSEWLTILGGFLGSLLFICSLTAFNNLENLVFGKGFQAKIFPEIVVCMFLALFASGLVHRVCVTTCLIFSVFALYYINKVSAALY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Zebrafish
Match: krtcap2 (keratinocyte associated protein 2 [Source:ZFIN;Acc:ZDB-GENE-060825-91])

HSP 1 Score: 87.0409 bits (214), Expect = 3.287e-22
Identity = 51/97 (52.58%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  148 FATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            FA +Q+F   + +S+ LTI GGF+ S +FI  LT FNN E ++FGKGFQ++IFPE+V+ + L+  A+G+VHR+C+T CL FSV  LYY+NK+S   Y
Sbjct:   20 FAGMQMFSRQLASSEWLTILGGFLGSLLFICSLTAFNNLENLVFGKGFQAKIFPEIVVCMFLALFASGLVHRVCVTTCLIFSVFALYYINKVSAALY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Zebrafish
Match: krtcap2 (keratinocyte associated protein 2 [Source:ZFIN;Acc:ZDB-GENE-060825-91])

HSP 1 Score: 87.0409 bits (214), Expect = 3.287e-22
Identity = 51/97 (52.58%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  148 FATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            FA +Q+F   + +S+ LTI GGF+ S +FI  LT FNN E ++FGKGFQ++IFPE+V+ + L+  A+G+VHR+C+T CL FSV  LYY+NK+S   Y
Sbjct:   20 FAGMQMFSRQLASSEWLTILGGFLGSLLFICSLTAFNNLENLVFGKGFQAKIFPEIVVCMFLALFASGLVHRVCVTTCLIFSVFALYYINKVSAALY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Mouse
Match: Krtcap2 (keratinocyte associated protein 2 [Source:MGI Symbol;Acc:MGI:1913309])

HSP 1 Score: 81.2629 bits (199), Expect = 6.637e-20
Identity = 45/97 (46.39%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  148 FATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            FA +Q++   + +++ LTI GG + S +F+  LT FNN E ++FGKGFQ++IFPE+++ L+L+  A+G++HR+C+T C  FS+ GLYY+NK+S   Y
Sbjct:   20 FAGMQIYSRQLASTEWLTIQGGLLGSGLFVFSLTAFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISSTLY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Mouse
Match: Krtcap2 (keratinocyte associated protein 2 [Source:MGI Symbol;Acc:MGI:1913309])

HSP 1 Score: 80.1073 bits (196), Expect = 1.153e-19
Identity = 33/63 (52.38%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:  250 VFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
             FNN E ++FGKGFQ++IFPE+++ L+L+  A+G++HR+C+T C  FS+ GLYY+NK+S   Y
Sbjct:   32 AFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISSTLY 94
[Close]
BLAST of Keratinocyte associated protein 2 vs. UniProt/SwissProt
Match: sp|Q1HQF8|KTAP2_AEDAE (Protein KRTCAP2 homolog OS=Aedes aegypti OX=7159 GN=AAEL007634 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 3.436e-26
Identity = 59/119 (49.58%), Postives = 87/119 (73.11%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP 447
            MA+  S S +LA   +++IF+ +Q++K  I +S++ T+FGGF+ S++FIL LT  +N E ++ GKGFQ+++FPEV   LI S  A GMVHR+C T C+ FSVA LYY+N++SQK +N P
Sbjct:    1 MAVPTSVSLVLASVTAVLIFSAMQMYKPLIASSQMATVFGGFLGSWLFILSLTAVSNLEAVVLGKGFQAKLFPEVAFCLIGSLFACGMVHRVCATTCILFSVAALYYINRISQKVHNAP 119
[Close]
BLAST of Keratinocyte associated protein 2 vs. UniProt/SwissProt
Match: sp|Q5Q995|KTAP2_IXOSC (Protein KRTCAP2 homolog OS=Ixodes scapularis OX=6945 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 8.675e-23
Identity = 54/117 (46.15%), Postives = 86/117 (73.50%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYN 441
            MA+S   S +LA  L +++FAT+Q++KS + +S+ + I GGF+ S +FIL+LT  +N E   FG+ FQ+++ PEVVI+L+++  A+GMVHR+CIT CL FS+  LYY++++S K + 
Sbjct:    1 MAVSSGTSGMLATCLFMLLFATMQIYKSQLTSSQPMAIVGGFLGSVLFILILTAISNFETHFFGRNFQTKLIPEVVIALVIAMAASGMVHRVCITTCLIFSIVALYYVSRISIKVHG 117
[Close]
BLAST of Keratinocyte associated protein 2 vs. UniProt/SwissProt
Match: sp|B2RZC9|KTAP2_RAT (Keratinocyte-associated protein 2 OS=Rattus norvegicus OX=10116 GN=Krtcap2 PE=2 SV=2)

HSP 1 Score: 81.6481 bits (200), Expect = 4.081e-19
Identity = 45/97 (46.39%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  148 FATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            FA +Q++   + +++ LTI GG + S +F+  LT FNN E ++FGKGFQ++IFPE+++ L+L+  A+G++HR+C+T C  FS+ GLYY+NK+S   Y
Sbjct:   20 FAGMQIYSRQLASTEWLTIQGGLLGSGLFVFSLTAFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISSTLY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. UniProt/SwissProt
Match: sp|Q5RL79|KTAP2_MOUSE (Keratinocyte-associated protein 2 OS=Mus musculus OX=10090 GN=Krtcap2 PE=1 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 4.643e-19
Identity = 45/97 (46.39%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  148 FATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            FA +Q++   + +++ LTI GG + S +F+  LT FNN E ++FGKGFQ++IFPE+++ L+L+  A+G++HR+C+T C  FS+ GLYY+NK+S   Y
Sbjct:   20 FAGMQIYSRQLASTEWLTIQGGLLGSGLFVFSLTAFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISSTLY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. UniProt/SwissProt
Match: sp|P86229|KTAP2_CANLF (Keratinocyte-associated protein 2 OS=Canis lupus familiaris OX=9615 GN=KRTCAP2 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 6.204e-19
Identity = 52/116 (44.83%), Postives = 83/116 (71.55%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            MA+    S  L+  LSL++FA +Q++   + +++ LTI GG + S +F+  LT FNN E ++FGKGFQ++IFPE+++ L+L+  A+G++HR+C+T C  FS+ GLYY+NK+S   Y
Sbjct:    1 MAVGTGTSLALSSLLSLLLFAGMQMYSRQLASTEWLTIQGGLLGSGLFVFSLTAFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISSTLY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. TrEMBL
Match: T1DFH0 (Putative plasma membrane protein OS=Psorophora albipes OX=869069 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 5.766e-25
Identity = 62/119 (52.10%), Postives = 87/119 (73.11%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP 447
            MA+  S S +LA   +++IF+ +Q++K  I +S+L TIFGG++ S++FIL LT  +N E ++ GKGFQS++FPEV   LI S  A GMVHR+C T CL FS+A LYY+NK+SQK +N P
Sbjct:    1 MAVPTSVSLVLASVTAVLIFSAMQMYKPLIASSQLATIFGGYLGSWLFILSLTAVSNLEAVVLGKGFQSKLFPEVAFCLIGSLFACGMVHRVCATTCLLFSIAALYYINKISQKVHNAP 119
[Close]
BLAST of Keratinocyte associated protein 2 vs. TrEMBL
Match: A5J298 (Keratinocyte associated protein 2 OS=Penaeus vannamei OX=6689 PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 1.683e-24
Identity = 56/113 (49.56%), Postives = 88/113 (77.88%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQ 429
            M +S  AS  +A+  S+++F+ LQ++K+ + +++L+T+  G+++S++FI +LT  NN E I+FGKGFQ+++ PEV + L+L+C AAGMVHR+ ITVCL  S+ GLYY+NKLSQ
Sbjct:    1 MGVSTGASFGMAILCSVLVFSGLQMYKNQLGSTRLMTLVAGYVASWLFIFMLTAVNNLENIMFGKGFQARVIPEVTLCLVLACAAAGMVHRVSITVCLLCSIVGLYYINKLSQ 113
[Close]
BLAST of Keratinocyte associated protein 2 vs. TrEMBL
Match: A0A2C9L3E5 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106077634 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.836e-24
Identity = 59/119 (49.58%), Postives = 88/119 (73.95%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP 447
            MALS + S++ ++ LS+I+FA +Q+FKS + + +  TI GGF+ S +FILLLT  +N E  LFGKGFQ+++FPEVV  L+ +  A+ +VHR+C+T CL FS+  LYY+N++SQ  Y +P
Sbjct:    1 MALSTAMSALTSLVLSIIVFALMQIFKSDLASKEYFTIAGGFVGSILFILLLTFTSNAEMFLFGKGFQARLFPEVVACLLTAMFASALVHRVCVTTCLIFSLVALYYINRISQTIYGIP 119
[Close]
BLAST of Keratinocyte associated protein 2 vs. TrEMBL
Match: A0A3R7P821 (Keratinocyte associated protein 2 OS=Penaeus vannamei OX=6689 GN=C7M84_003197 PE=4 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.195e-23
Identity = 57/118 (48.31%), Postives = 90/118 (76.27%), Query Frame = 1 
Query:   76 DFRKFMALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQ 429
            +FR    +S  AS  +A+  S+++F+ LQ++K+ + +++L+T+  G+++S++FI +LT  NN E I+FGKGFQ+++ PEV + L+L+C AAGMVHR+ ITVCL  S+ GLYY+NKLSQ
Sbjct:    9 EFR----VSTGASFGMAILCSVLVFSGLQMYKNQLGSTRLMTLVAGYVASWLFIFMLTAVNNLENIMFGKGFQARVIPEVTLCLVLACAAAGMVHRVSITVCLLCSIVGLYYINKLSQ 122
[Close]
BLAST of Keratinocyte associated protein 2 vs. TrEMBL
Match: A0A023EFR5 (Uncharacterized protein OS=Aedes albopictus OX=7160 GN=109408493 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.409e-23
Identity = 60/119 (50.42%), Postives = 87/119 (73.11%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP 447
            MA+  S S +LA   +++IF+ +Q+++  I +S++ TIFGGF+ S++FIL LT  +N E ++ GKGFQ+++FPEV   LI S  A GMVHR+C T C+ FSVA LYY+NK+SQK +N P
Sbjct:    1 MAVPTSVSLVLASVTAVLIFSAMQMYRPLIASSQMATIFGGFLGSWLFILSLTAVSNLEAVVLGKGFQAKLFPEVAFCLIGSLFACGMVHRVCATTCVLFSVAALYYINKISQKVHNAP 119
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Cavefish
Match: krtcap2 (keratinocyte associated protein 2 [Source:NCBI gene;Acc:103022799])

HSP 1 Score: 85.5001 bits (210), Expect = 1.407e-21
Identity = 50/97 (51.55%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:  148 FATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            FA +Q+F   + +S+ LTI GGF+ S +F+  LT FNN E ++FGKGFQ++IFPE+++ L LS  A+G+VHR+C+T CL FS+  LYY+NK+S   Y
Sbjct:   20 FAGMQMFSRQLGSSEWLTILGGFLGSVLFVCSLTAFNNLENLVFGKGFQAKIFPEILLCLCLSLFASGLVHRVCVTTCLIFSLFALYYINKVSAALY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Nematostella
Match: EDO37222 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGC5])

HSP 1 Score: 87.4261 bits (215), Expect = 7.686e-23
Identity = 52/116 (44.83%), Postives = 82/116 (70.69%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            MAL  + S + A TL+++ FA +Q+FK+ +  S+ +TI GGFI S +F+ LLT   N E ++FG+GF +++FPEV+I L ++  A+G+VHR+C+T C  FS+  LYYLN++S   +
Sbjct:    1 MALPTATSFLFASTLTVLTFALMQMFKTNLGGSEWMTILGGFIGSQLFVFLLTAIGNFESMIFGRGFHTKLFPEVLICLTVAMFASGLVHRVCVTTCFLFSMVALYYLNRISSSVH 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. Ensembl Medaka
Match: krtcap2 (keratinocyte associated protein 2 [Source:NCBI gene;Acc:101155632])

HSP 1 Score: 83.9593 bits (206), Expect = 4.517e-21
Identity = 55/116 (47.41%), Postives = 83/116 (71.55%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFY 438
            MA++   S +L+  LS+++FA +Q+F   + +++  TI GGF+ S +FI  LT FNN E ++FGKGFQ++IFPE++  L LS  A+G+VHR+C+T CL FS+  LYY+NK+S   Y
Sbjct:    1 MAVNTGTSLVLSSLLSVLVFAGMQMFSKQLASTEWFTILGGFLGSVLFICSLTAFNNLENLVFGKGFQAKIFPEILFCLCLSMFASGLVHRVCVTTCLIFSLFALYYINKISAALY 116
[Close]
BLAST of Keratinocyte associated protein 2 vs. Planmine SMEST
Match: SMESG000055989.1 (SMESG000055989.1)

HSP 1 Score: 207.994 bits (528), Expect = 6.730e-70
Identity = 125/126 (99.21%), Postives = 125/126 (99.21%), Query Frame = 1 
Query:   70 MVDFRKFMALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP 447
            M DFRKFMALSHSASSILAMTLSLIIFATLQVFKSYIIASKLLTIFGGFISSFIFILLLTVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP
Sbjct:    1 MFDFRKFMALSHSASSILAMTLSLIIFATLQVFKSYIIASKLLTIFGGFISSFIFILLLTVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP 126
[Close]
BLAST of Keratinocyte associated protein 2 vs. Planmine SMEST
Match: SMESG000055989.1 (SMESG000055989.1)

HSP 1 Score: 194.512 bits (493), Expect = 1.116e-64
Identity = 119/119 (100.00%), Postives = 119/119 (100.00%), Query Frame = 1 
Query:   91 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLXXXXXXXXXXXXXXXXXXVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP 447
            MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLLTIFGGFISSFIFILLLTVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP
Sbjct:    1 MALSHSASSILAMTLSLIIFATLQVFKSYIIASKLLTIFGGFISSFIFILLLTVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRICITVCLFFSVAGLYYLNKLSQKFYNVP 119
The following BLAST results are available for this feature:
BLAST of Keratinocyte associated protein 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
KRTCAP21.979e-1946.39keratinocyte associated protein 2 [Source:HGNC Sym... [more]
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG314608.886e-1941.28gene:FBgn0051460 transcript:FBtr0081873[more]
CG314608.886e-1941.28gene:FBgn0051460 transcript:FBtr0339327[more]
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
krtcap23.287e-2252.58keratinocyte associated protein 2 [Source:ZFIN;Acc... [more]
krtcap23.287e-2252.58keratinocyte associated protein 2 [Source:ZFIN;Acc... [more]
krtcap23.287e-2252.58keratinocyte associated protein 2 [Source:ZFIN;Acc... [more]
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Krtcap26.637e-2046.39keratinocyte associated protein 2 [Source:MGI Symb... [more]
Krtcap21.153e-1952.38keratinocyte associated protein 2 [Source:MGI Symb... [more]
back to top
BLAST of Keratinocyte associated protein 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q1HQF8|KTAP2_AEDAE3.436e-2649.58Protein KRTCAP2 homolog OS=Aedes aegypti OX=7159 G... [more]
sp|Q5Q995|KTAP2_IXOSC8.675e-2346.15Protein KRTCAP2 homolog OS=Ixodes scapularis OX=69... [more]
sp|B2RZC9|KTAP2_RAT4.081e-1946.39Keratinocyte-associated protein 2 OS=Rattus norveg... [more]
sp|Q5RL79|KTAP2_MOUSE4.643e-1946.39Keratinocyte-associated protein 2 OS=Mus musculus ... [more]
sp|P86229|KTAP2_CANLF6.204e-1944.83Keratinocyte-associated protein 2 OS=Canis lupus f... [more]
back to top
BLAST of Keratinocyte associated protein 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1DFH05.766e-2552.10Putative plasma membrane protein OS=Psorophora alb... [more]
A5J2981.683e-2449.56Keratinocyte associated protein 2 OS=Penaeus vanna... [more]
A0A2C9L3E52.836e-2449.58Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A3R7P8211.195e-2348.31Keratinocyte associated protein 2 OS=Penaeus vanna... [more]
A0A023EFR51.409e-2350.42Uncharacterized protein OS=Aedes albopictus OX=716... [more]
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
krtcap21.407e-2151.55keratinocyte associated protein 2 [Source:NCBI gen... [more]
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO372227.686e-2344.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Keratinocyte associated protein 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
krtcap24.517e-2147.41keratinocyte associated protein 2 [Source:NCBI gen... [more]
back to top
BLAST of Keratinocyte associated protein 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000055989.16.730e-7099.21SMESG000055989.1[more]
SMESG000055989.11.116e-64100.00SMESG000055989.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010216 ID=SMED30010216|Name=Keratinocyte associated protein 2|organism=Schmidtea mediterranea sexual|type=transcript|length=489bp
GTTAAATTGCATAATCAAATTAAATAACTTTAAATATTTCCATTTATTTT
AATATTATAAATTAATATAATGGTTGACTTTAGAAAATTTATGGCATTGT
CACATAGTGCTTCATCTATATTGGCCATGACATTATCTTTGATAATTTTT
GCCACTCTTCAAGTTTTTAAATCCTATATAATTGCAAGTAAATTACTAAC
TATCTTCGGTGGATTTATTAGTAGCTTTATATTCATTCTTCTTTTAACAG
TTTTTAATAATTGTGAACAAATTCTATTTGGCAAGGGATTTCAATCACAA
ATATTTCCAGAAGTCGTTATTTCGTTGATTTTGTCATGCATAGCAGCAGG
AATGGTACATAGAATTTGTATAACAGTTTGTTTATTTTTCTCTGTGGCTG
GATTGTATTATTTGAATAAACTTTCTCAAAAGTTTTACAATGTTCCAACA
ACGGCAACATCAACGAAATCAAGCAAGAAAAAAAAATAG
back to top

protein sequence of SMED30010216-orf-1

>SMED30010216-orf-1 ID=SMED30010216-orf-1|Name=SMED30010216-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=140bp
MVDFRKFMALSHSASSILAMTLSLIIFATLQVFKSYIIASKLLTIFGGFI
SSFIFILLLTVFNNCEQILFGKGFQSQIFPEVVISLILSCIAAGMVHRIC
ITVCLFFSVAGLYYLNKLSQKFYNVPTTATSTKSSKKKK*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR018614KRTCAP2
Vocabulary: cellular component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018614Keratinocyte-associated protein 2PFAMPF09775Keratin_assoccoord: 11..138
e-value: 9.1E-46
score: 154.7
IPR018614Keratinocyte-associated protein 2PANTHERPTHR32001FAMILY NOT NAMEDcoord: 8..139
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
NoneNo IPR availableTMHMMTMhelixcoord: 42..62
NoneNo IPR availableTMHMMTMhelixcoord: 82..115