pim-2

Overview
Namepim-2 (AFJ24850.1)
Smed IDSMED30010205
Length (bp)1037
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of pim-2 (SMED30010205) t-SNE clustered cells

Violin plots show distribution of expression levels for pim-2 (SMED30010205) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of pim-2 (SMED30010205) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for pim-2 (SMED30010205) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010205

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30010205h1SMcG0003234 dd_Smed_v4_4435_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010205h1SMcG0003234 dd_Smed_v4_4435_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010205h1SMcG0003234 dd_Smed_v4_4435_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010205h1SMcG0003234 dd_Smed_v4_4435_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010205h1SMcG0003234 dd_Smed_v4_4435_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of pim-2 vs. Ensembl Human
Match: PIM1 (Pim-1 proto-oncogene, serine/threonine kinase [Source:HGNC Symbol;Acc:HGNC:8986])

HSP 1 Score: 178.718 bits (452), Expect = 4.621e-53
Identity = 105/273 (38.46%), Postives = 154/273 (56.41%), Query Frame = 3 
Query:  183 SKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQ-GIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            + KL      + ++++Y +   +G GGFG V+   ++S+   VA+K V KD+IS++        ++PME VLL +   G  GVI+LL+  E      +  ++ER     DLFD+I     + EE A     Q++  +  CH  GV HRDIKDENILID++  E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+++ EI+   +      S+EC+ LI  CL     +R   ++I  H   Q
Sbjct:   22 ATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWG-ELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERP--DSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQ 291
[Close]
BLAST of pim-2 vs. Ensembl Human
Match: PIM3 (Pim-3 proto-oncogene, serine/threonine kinase [Source:HGNC Symbol;Acc:HGNC:19310])

HSP 1 Score: 173.711 bits (439), Expect = 5.860e-51
Identity = 96/264 (36.36%), Postives = 144/264 (54.55%), Query Frame = 3 
Query:  198 VKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXX-XXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEI-LLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCH 983
             K+  +  +  Y +   +G GGFG V+  ++I++G  VA+K V K++++E+         +P+E VLL +     G   ++ +++  E    +  V+ER     DLFD+I     + E  A     Q++  +  CH  GV HRDIKDEN+L+D+ + E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+ + EI     +     S EC+ LI  CL      R  LD+I  H
Sbjct:   29 AKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVVKERVTEW--GSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHSCGVVHRDIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSERPSLDQIAAH 290
[Close]
BLAST of pim-2 vs. Ensembl Human
Match: PIM2 (Pim-2 proto-oncogene, serine/threonine kinase [Source:HGNC Symbol;Acc:HGNC:8987])

HSP 1 Score: 160.999 bits (406), Expect = 3.012e-46
Identity = 99/262 (37.79%), Postives = 144/262 (54.96%), Query Frame = 3 
Query:  222 QNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQ---GIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            + +Y +   +G+GGFG VF   ++++   VA+K + ++++  +    ++    P+E  LL +     G PGVI+LL+  E +  + +  V+ER     DLFDYI     + E  +     Q++  +  CH  GV HRDIKDENILID+     KLIDFGSG   HD  Y DF+GT +YSPPEWI    Y       WS+G LLY+M+ GD PF+ + EIL A +   +  S +C  LI  CL     +R  L++IL     Q
Sbjct:   29 EAEYRLGPLLGKGGFGTVFAGHRLTDRLQVAIKVIPRNRVLGWSPLSDSV-TCPLEVALLWKVGAGGGHPGVIRLLDWFETQ--EGFMLVLERPLPAQDLFDYITEKGPLGEGPSRCFFGQVVAAIQHCHSRGVVHRDIKDENILIDLRRGCAKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWSLGILLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPSSRPSLEEILLDPWMQ 287
[Close]
BLAST of pim-2 vs. Ensembl Human
Match: PIM2 (Pim-2 proto-oncogene, serine/threonine kinase [Source:HGNC Symbol;Acc:HGNC:8987])

HSP 1 Score: 129.798 bits (325), Expect = 2.742e-36
Identity = 65/134 (48.51%), Postives = 79/134 (58.96%), Query Frame = 3 
Query:  477 FVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLA 878
             V+ER     DLFDYI     + E  +     Q++  +  CH  GV HRDIKDENILID+     KLIDFGSG   HD  Y DF+GT +YSPPEWI    Y       WS+G LLY+M+ GD PF+ + EIL A
Sbjct:    2 LVLERPLPAQDLFDYITEKGPLGEGPSRCFFGQVVAAIQHCHSRGVVHRDIKDENILIDLRRGCAKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWSLGILLYDMVCGDIPFERDQEILEA 135
[Close]
BLAST of pim-2 vs. Ensembl Human
Match: PASK (PAS domain containing serine/threonine kinase [Source:HGNC Symbol;Acc:HGNC:17270])

HSP 1 Score: 118.627 bits (296), Expect = 1.150e-28
Identity = 84/253 (33.20%), Postives = 127/253 (50.20%), Query Frame = 3 
Query:  228 KYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQ-QKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGE-YFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKIL 977
            KY+    +G G FG V+ +      K V +KF+ K+K+ E C  E+ +  K+ +E  +L+  +    +  L        FQ+   VME++ + +DLF +I+R  ++ E  A +I  QL+  +       + HRDIKDENI+I  D   IKLIDFGS  Y   G+ ++ F GT  Y  PE +    Y   ELE WS+G  LY ++  + PF    E + A I      S E   L+   L    E R  L+K++
Sbjct:  998 KYSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQL---VMEKHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFT-IKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLV 1246
[Close]
BLAST of pim-2 vs. Ensembl Celegans
Match: prk-2 (Serine/threonine-protein kinase prk-2 [Source:UniProtKB/Swiss-Prot;Acc:Q20443])

HSP 1 Score: 174.096 bits (440), Expect = 2.221e-50
Identity = 101/265 (38.11%), Postives = 150/265 (56.60%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEI----LLAPIS-IDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMS 1010
            Y +  ++G GGFG V+++ +  +   VA+KF+ +  + E+       +++PME  +LA+C  + GVI+LL+   +   + +  VMER    ID+FD+I    +ISE+ A F+  Q+  T+  C +  V HRD+KDENI+ID+ T   KLIDFG+       +Y DF GT +Y PPEW     Y  +E   WS+G LLYN + G  PF+NE +I    LL P+      SAE +DLI  CL  D   R  L+ IL H   ++  +S
Sbjct:   31 YKLKAELGRGGFGVVYRAVRTCDNALVAVKFIERSNVKEWA--RINGEQVPMEICMLAKCSKVRGVIRLLDWYSIP--EGFLIVMERPYPCIDMFDFIKGQGKISEDMARFLFRQIAVTVHECVQNRVLHRDLKDENIVIDLVTGSTKLIDFGAATVLRRSQYSDFQGTRLYCPPEWFLHSLYLGREAAVWSLGVLLYNSLNGRLPFRNEKDICTAHLLGPLPFFVPVSAEVKDLISKCLTFDPFQRCSLEAILNHPWVKQQTLS 291
[Close]
BLAST of pim-2 vs. Ensembl Celegans
Match: prk-2 (Serine/threonine-protein kinase prk-2 [Source:UniProtKB/Swiss-Prot;Acc:Q20443])

HSP 1 Score: 174.096 bits (440), Expect = 2.221e-50
Identity = 101/265 (38.11%), Postives = 150/265 (56.60%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEI----LLAPIS-IDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMS 1010
            Y +  ++G GGFG V+++ +  +   VA+KF+ +  + E+       +++PME  +LA+C  + GVI+LL+   +   + +  VMER    ID+FD+I    +ISE+ A F+  Q+  T+  C +  V HRD+KDENI+ID+ T   KLIDFG+       +Y DF GT +Y PPEW     Y  +E   WS+G LLYN + G  PF+NE +I    LL P+      SAE +DLI  CL  D   R  L+ IL H   ++  +S
Sbjct:   31 YKLKAELGRGGFGVVYRAVRTCDNALVAVKFIERSNVKEWA--RINGEQVPMEICMLAKCSKVRGVIRLLDWYSIP--EGFLIVMERPYPCIDMFDFIKGQGKISEDMARFLFRQIAVTVHECVQNRVLHRDLKDENIVIDLVTGSTKLIDFGAATVLRRSQYSDFQGTRLYCPPEWFLHSLYLGREAAVWSLGVLLYNSLNGRLPFRNEKDICTAHLLGPLPFFVPVSAEVKDLISKCLTFDPFQRCSLEAILNHPWVKQQTLS 291
[Close]
BLAST of pim-2 vs. Ensembl Celegans
Match: prk-1 (Pim (Mammalian oncogene) Related Kinase [Source:UniProtKB/TrEMBL;Acc:K8ESD7])

HSP 1 Score: 168.318 bits (425), Expect = 9.532e-49
Identity = 100/264 (37.88%), Postives = 150/264 (56.82%), Query Frame = 3 
Query:  201 KSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKS-VALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCH-KAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCH 983
            K +VK  + KY +  +IG GGFG V+++T   +G+  VA+KFV    +  + +    +Q IP E   L  C+ IPGVIK+L+       + +  VMER  N +DLFD ++    ++E+   FI  Q+I T+   + K G+ HRDIKDEN++++++T E+KL+DFG+  Y       +F GT  Y PPEW +D+ Y   E  SWS+G LL+ ++TG  PF+NE +I L  +      S E   L+ SCL      R  L +I  H
Sbjct:   24 KHSVKEFKRKYEVLDEIGRGGFGIVYEATTRQDGQQPVAVKFVQHKHVRSWTM--TCRQLIPSEVCHLETCEDIPGVIKILDWFANS--KGFLIVMERPANCMDLFDMVSVHGPLNEDMGKFIFKQVITTVFNMYSKHGLLHRDIKDENLIVNMNTGEVKLVDFGATAYAEKATKKEFQGTRSYCPPEWFRDQLYLPLEATSWSLGVLLFILLTGKLPFRNEIQICLGNVKFPPDLSKEVCQLVKSCLTTSTSARASLAQIAAH 283
[Close]
BLAST of pim-2 vs. Ensembl Celegans
Match: prk-1 (Pim (Mammalian oncogene) Related Kinase [Source:UniProtKB/TrEMBL;Acc:K8ESD7])

HSP 1 Score: 167.933 bits (424), Expect = 4.477e-47
Identity = 100/264 (37.88%), Postives = 150/264 (56.82%), Query Frame = 3 
Query:  201 KSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKS-VALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCH-KAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCH 983
            K +VK  + KY +  +IG GGFG V+++T   +G+  VA+KFV    +  + +    +Q IP E   L  C+ IPGVIK+L+       + +  VMER  N +DLFD ++    ++E+   FI  Q+I T+   + K G+ HRDIKDEN++++++T E+KL+DFG+  Y       +F GT  Y PPEW +D+ Y   E  SWS+G LL+ ++TG  PF+NE +I L  +      S E   L+ SCL      R  L +I  H
Sbjct:   24 KHSVKEFKRKYEVLDEIGRGGFGIVYEATTRQDGQQPVAVKFVQHKHVRSWTM--TCRQLIPSEVCHLETCEDIPGVIKILDWFANS--KGFLIVMERPANCMDLFDMVSVHGPLNEDMGKFIFKQVITTVFNMYSKHGLLHRDIKDENLIVNMNTGEVKLVDFGATAYAEKATKKEFQGTRSYCPPEWFRDQLYLPLEATSWSLGVLLFILLTGKLPFRNEIQICLGNVKFPPDLSKEVCQLVKSCLTTSTSARASLAQIAAH 283
[Close]
BLAST of pim-2 vs. Ensembl Celegans
Match: prk-1 (Pim (Mammalian oncogene) Related Kinase [Source:UniProtKB/TrEMBL;Acc:K8ESD7])

HSP 1 Score: 167.933 bits (424), Expect = 5.248e-47
Identity = 100/264 (37.88%), Postives = 150/264 (56.82%), Query Frame = 3 
Query:  201 KSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKS-VALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCH-KAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCH 983
            K +VK  + KY +  +IG GGFG V+++T   +G+  VA+KFV    +  + +    +Q IP E   L  C+ IPGVIK+L+       + +  VMER  N +DLFD ++    ++E+   FI  Q+I T+   + K G+ HRDIKDEN++++++T E+KL+DFG+  Y       +F GT  Y PPEW +D+ Y   E  SWS+G LL+ ++TG  PF+NE +I L  +      S E   L+ SCL      R  L +I  H
Sbjct:   47 KHSVKEFKRKYEVLDEIGRGGFGIVYEATTRQDGQQPVAVKFVQHKHVRSWTM--TCRQLIPSEVCHLETCEDIPGVIKILDWFANS--KGFLIVMERPANCMDLFDMVSVHGPLNEDMGKFIFKQVITTVFNMYSKHGLLHRDIKDENLIVNMNTGEVKLVDFGATAYAEKATKKEFQGTRSYCPPEWFRDQLYLPLEATSWSLGVLLFILLTGKLPFRNEIQICLGNVKFPPDLSKEVCQLVKSCLTTSTSARASLAQIAAH 306
[Close]
BLAST of pim-2 vs. Ensembl Fly
Match: Pask (gene:FBgn0034950 transcript:FBtr0072160)

HSP 1 Score: 110.923 bits (276), Expect = 2.217e-26
Identity = 84/271 (31.00%), Postives = 141/271 (52.03%), Query Frame = 3 
Query:  174 SKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKI-SEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKIL 977
            SK+  +L +    KY    Y    +IG G +G V  + + S+   V  KF+ K+K+ S++ +     +++P+E  LL   Q +     +  +   E    +  VME++ + +DL+ +I R   + E+   +I  Q+   +   H+  + HRDIKDENI+ID +   IKLIDFGS  +  +G++F  F GT+ Y  PE +    Y   ELE W++G  LY ++  + PF +  E L A I I  A S +   L+ S L +D + R  + +++
Sbjct:  561 SKYEDELYLGDYSKY----YTSIRQIGRGAYGYVNMAFRNSDRLLVITKFILKEKLCSQFMVKSRDCKEVPIEIHLL---QTLNHKNIVSVLDVFENDLFYQLVMEKHGSGMDLWTFIERRPLMDEKLGSYIFRQVADAVNYLHEQKILHRDIKDENIIIDQNFT-IKLIDFGSATFMEEGKFFSTFYGTTEYCSPEVLAGNRYVGPELEIWALGVTLYVLMFFENPFIDVEETLKAEIQIPKAVSEQLSRLLSSMLNKDPKYRCTMHQLI 823
[Close]
BLAST of pim-2 vs. Ensembl Fly
Match: Sik3 (gene:FBgn0262103 transcript:FBtr0344259)

HSP 1 Score: 100.138 bits (248), Expect = 9.653e-23
Identity = 76/266 (28.57%), Postives = 126/266 (47.37%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKN------ENEILLAPISID-SASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYH 1004
            Y +   IG+G F  V  +T I     VA+K + K      CL+EE   K   E  +L   + +           +E   +   V E  PN  ++FD++    ++ E  A  + TQL+  +  CH+ GV HRD+K EN+L+D D   IKL DFG  ++Y +G     + G+  Y+ PE  +   Y   + + WS+G +LY ++ G  PF        ++ ++L    I    S EC  LI + L  + + R  + +I+ H+   ++ 
Sbjct:   41 YELEKTIGKGNFAVVKLATNIVTKTKVAIKIIDKT-----CLNEEYLNKTFREIAIL---KSLRHPHITRLYEVMESQSMIYLVTEYAPNG-EIFDHLVANGRMKEPEAARVFTQLVSAVHYCHRRGVVHRDLKAENVLLDKDMN-IKLADFGFSNHYEEGATLKTWCGSPPYAAPEVFQGLEYDGPKSDIWSLGVVLYALVCGALPFDGKTILELKSRVVLGKFRIPFFMSQECEQLIRNMLVVEPDRRYTIKQIIKHRWLSEWQ 296
[Close]
BLAST of pim-2 vs. Ensembl Fly
Match: Sik3 (gene:FBgn0262103 transcript:FBtr0344258)

HSP 1 Score: 100.138 bits (248), Expect = 9.657e-23
Identity = 76/266 (28.57%), Postives = 126/266 (47.37%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKN------ENEILLAPISID-SASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYH 1004
            Y +   IG+G F  V  +T I     VA+K + K      CL+EE   K   E  +L   + +           +E   +   V E  PN  ++FD++    ++ E  A  + TQL+  +  CH+ GV HRD+K EN+L+D D   IKL DFG  ++Y +G     + G+  Y+ PE  +   Y   + + WS+G +LY ++ G  PF        ++ ++L    I    S EC  LI + L  + + R  + +I+ H+   ++ 
Sbjct:   41 YELEKTIGKGNFAVVKLATNIVTKTKVAIKIIDKT-----CLNEEYLNKTFREIAIL---KSLRHPHITRLYEVMESQSMIYLVTEYAPNG-EIFDHLVANGRMKEPEAARVFTQLVSAVHYCHRRGVVHRDLKAENVLLDKDMN-IKLADFGFSNHYEEGATLKTWCGSPPYAAPEVFQGLEYDGPKSDIWSLGVVLYALVCGALPFDGKTILELKSRVVLGKFRIPFFMSQECEQLIRNMLVVEPDRRYTIKQIIKHRWLSEWQ 296
[Close]
BLAST of pim-2 vs. Ensembl Fly
Match: Sik3 (gene:FBgn0262103 transcript:FBtr0340388)

HSP 1 Score: 100.138 bits (248), Expect = 9.783e-23
Identity = 76/266 (28.57%), Postives = 126/266 (47.37%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKN------ENEILLAPISID-SASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYH 1004
            Y +   IG+G F  V  +T I     VA+K + K      CL+EE   K   E  +L   + +           +E   +   V E  PN  ++FD++    ++ E  A  + TQL+  +  CH+ GV HRD+K EN+L+D D   IKL DFG  ++Y +G     + G+  Y+ PE  +   Y   + + WS+G +LY ++ G  PF        ++ ++L    I    S EC  LI + L  + + R  + +I+ H+   ++ 
Sbjct:   41 YELEKTIGKGNFAVVKLATNIVTKTKVAIKIIDKT-----CLNEEYLNKTFREIAIL---KSLRHPHITRLYEVMESQSMIYLVTEYAPNG-EIFDHLVANGRMKEPEAARVFTQLVSAVHYCHRRGVVHRDLKAENVLLDKDMN-IKLADFGFSNHYEEGATLKTWCGSPPYAAPEVFQGLEYDGPKSDIWSLGVVLYALVCGALPFDGKTILELKSRVVLGKFRIPFFMSQECEQLIRNMLVVEPDRRYTIKQIIKHRWLSEWQ 296
[Close]
BLAST of pim-2 vs. Ensembl Fly
Match: Sik3 (gene:FBgn0262103 transcript:FBtr0304026)

HSP 1 Score: 100.138 bits (248), Expect = 9.788e-23
Identity = 76/266 (28.57%), Postives = 126/266 (47.37%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKN------ENEILLAPISID-SASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYH 1004
            Y +   IG+G F  V  +T I     VA+K + K      CL+EE   K   E  +L   + +           +E   +   V E  PN  ++FD++    ++ E  A  + TQL+  +  CH+ GV HRD+K EN+L+D D   IKL DFG  ++Y +G     + G+  Y+ PE  +   Y   + + WS+G +LY ++ G  PF        ++ ++L    I    S EC  LI + L  + + R  + +I+ H+   ++ 
Sbjct:   41 YELEKTIGKGNFAVVKLATNIVTKTKVAIKIIDKT-----CLNEEYLNKTFREIAIL---KSLRHPHITRLYEVMESQSMIYLVTEYAPNG-EIFDHLVANGRMKEPEAARVFTQLVSAVHYCHRRGVVHRDLKAENVLLDKDMN-IKLADFGFSNHYEEGATLKTWCGSPPYAAPEVFQGLEYDGPKSDIWSLGVVLYALVCGALPFDGKTILELKSRVVLGKFRIPFFMSQECEQLIRNMLVVEPDRRYTIKQIIKHRWLSEWQ 296
[Close]
BLAST of pim-2 vs. Ensembl Zebrafish
Match: pim2 (Pim-2 proto-oncogene, serine/threonine kinase [Source:NCBI gene;Acc:58054])

HSP 1 Score: 183.341 bits (464), Expect = 4.820e-55
Identity = 103/271 (38.01%), Postives = 153/271 (56.46%), Query Frame = 3 
Query:  192 LVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQG----IPGXXXXXXXXXXXXF--QVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCH 983
            L  K+  ++ + +Y +   +G GGFG V+   +IS+G+ VA+K +++D+I ++         +P E  LL    G    +PG   ++ +++  E   Q +  V E+  +  DLFD+I     + E  A   L Q+I  +  CH  G+ HRDIKDENIL+D  T +IK+IDFGSG    D  Y DF GT +YSPPEWI    Y    L  WS+G LLY+M+ GD PF+ + +I+ A  S +   S +CR LI SCL  +  +R  L++IL H
Sbjct:   17 LKAKNGKEHFEKQYTMGNLLGSGGFGSVYSGHRISDGQKVAIKQISRDRIQQWSKMPGEVNPVPNEIALLQSLGGGSGSVPGHRGIIRMLDWFEIPGQEYLIVFEKPQHCQDLFDFITERGALDESLARRFLKQVIEAVQFCHSKGIVHRDIKDENILVDTRTGDIKVIDFGSGATLKDSMYTDFEGTRVYSPPEWILYHKYHALPLTVWSLGVLLYDMVCGDIPFEQDTDIVKAKPSFNKRISNDCRSLICSCLSFNPGDRPSLEQILQH 287
[Close]
BLAST of pim-2 vs. Ensembl Zebrafish
Match: pim1 (Pim-1 proto-oncogene, serine/threonine kinase [Source:ZFIN;Acc:ZDB-GENE-061013-139])

HSP 1 Score: 181.415 bits (459), Expect = 3.279e-54
Identity = 107/257 (41.63%), Postives = 147/257 (57.20%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAEC-QGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            Y +   +G GGFG V+   +I++G  VA+K VAKD+++E+        ++PME VLL +   G  GVIK+L+  E      +  VMER     DLFD+I     + EE A     Q++  +  CH +GV HRDIKDENILID+ T ++KLIDFGSG    D  Y DF+GT +YSPPEWIK   Y  +    WS+G LLY+M+ GD PF+ + EI+   +      S EC+ LI  CL     +R   + I+ H   Q
Sbjct:   35 YQVGTVLGSGGFGTVYAGLRIADGAPVAIKHVAKDRVTEWG-ELPNGARVPMEIVLLKKVGTGFRGVIKMLDWYERP--DSFIIVMERPEVVKDLFDFITEKGALPEELARNFFRQVLEAVRHCHNSGVVHRDIKDENILIDLRTGDLKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIKFHRYHGRSATVWSLGILLYDMVCGDIPFEQDEEIVRGQVLFRRRISTECQQLIKWCLALRPADRPSFEDIINHDWMQ 288
[Close]
BLAST of pim-2 vs. Ensembl Zebrafish
Match: pim3 (Pim-3 proto-oncogene, serine/threonine kinase [Source:ZFIN;Acc:ZDB-GENE-050809-111])

HSP 1 Score: 176.792 bits (447), Expect = 1.954e-52
Identity = 101/266 (37.97%), Postives = 147/266 (55.26%), Query Frame = 3 
Query:  201 KSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEI-LLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            K+  +  +  Y +   +G GGFG V+  T+IS+   VA+K VAK++++E+     +   IP+E VLL +  G      +  I   E    W  +MER     DLFDYI     + E+TA     Q++  +  C+  GV HRDIKDEN+L+D+ T ++KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+++ EI           S  C+ LI  CLC    +R  L++I  H+  +
Sbjct:   31 KTEREPFEKVYHVGSVLGSGGFGTVYAGTRISDALPVAIKHVAKERVTEWGTINGSL--IPLEIVLLKKV-GSTFQGVIKLIDWYERPDGWLIIMERPEMVKDLFDYITEKGALDEDTARGFFRQVLEAVRHCYSCGVVHRDIKDENLLVDLRTGDLKLIDFGSGAILKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEHDEEILRGRLFFRRRVSPVCQQLIKWCLCLRPSDRPTLEQIFEHQWMR 293
[Close]
BLAST of pim-2 vs. Ensembl Zebrafish
Match: pimr25 (Pim proto-oncogene, serine/threonine kinase, related 25 [Source:ZFIN;Acc:ZDB-GENE-131125-30])

HSP 1 Score: 148.288 bits (373), Expect = 1.969e-41
Identity = 91/281 (32.38%), Postives = 134/281 (47.69%), Query Frame = 3 
Query:  168 NHSKFSKKLVVKSTVKYIQNKYAIYGK---IGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPG-XXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEI-----LLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCH 983
              S   + L   S+    +++  +Y K   +G GGFG VF   + S+G  VA+K+V+KD+  E  L  + Q ++P+E  L+      P     L  +   +  + +  ++ER     DL  +      + E  A  +L QLI  L  C   GV HRD+K EN+LI  ++++IKL+DFG G       Y  F GT  Y+PPEW +   Y       WS+G  LYN++    PF+    +     L  P S+   S ECR LI  CL     +R  LD I  H
Sbjct:   51 RQSSNQQTLSTSSSRPAKRSRRDLYQKGPLLGRGGFGSVFAGMRRSDGLPVAIKYVSKDRTPER-LKVDGQGRLPLEVALMTRVTSAPACPSVLQLLDWFDRPRRYILILERPDPCQDLQSFCEENGCLDERLAKKVLVQLIAALKHCESRGVLHRDVKPENLLISTESQDIKLLDFGCGDLLKRSAYKYFAGTPAYAPPEWFRRHRYHATPATVWSVGVTLYNILCDRFPFRGAQRVTSRSRLTFPRSL---STECRQLIRWCLSAAPADRPSLDDIESH 327
[Close]
BLAST of pim-2 vs. Ensembl Zebrafish
Match: pimr24 (Pim proto-oncogene, serine/threonine kinase, related 24 [Source:ZFIN;Acc:ZDB-GENE-131120-2])

HSP 1 Score: 148.288 bits (373), Expect = 2.097e-41
Identity = 91/281 (32.38%), Postives = 134/281 (47.69%), Query Frame = 3 
Query:  168 NHSKFSKKLVVKSTVKYIQNKYAIYGK---IGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPG-XXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEI-----LLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCH 983
              S   + L   S+    +++  +Y K   +G GGFG VF   + S+G  VA+K+V+KD+  E  L  + Q ++P+E  L+      P     L  +   +  + +  ++ER     DL  +      + E  A  +L QLI  L  C   GV HRD+K EN+LI  ++++IKL+DFG G       Y  F GT  Y+PPEW +   Y       WS+G  LYN++    PF+    +     L  P S+   S ECR LI  CL     +R  LD I  H
Sbjct:   52 RQSSNQQTLSTSSSRPAKRSRRDLYQKGPLLGRGGFGSVFAGMRRSDGLPVAIKYVSKDRTPER-LKVDGQGRLPLEVALMTRVTSAPACPSVLQLLDWFDRPRRYILILERPDPCQDLQSFCEENGCLDERLAKKVLVQLIAALKHCESRGVLHRDVKPENLLISTESQDIKLLDFGCGDLLKRSAYKYFAGTPAYAPPEWFRRHRYHATPATVWSVGVTLYNIMCDRFPFRGAQRVTSRSRLTFPRSL---STECRQLIRWCLSAAPADRPSLDDIESH 328
[Close]
BLAST of pim-2 vs. Ensembl Xenopus
Match: ncbp1 (nuclear cap binding protein subunit 1 [Source:Xenbase;Acc:XB-GENE-5841194])

HSP 1 Score: 179.104 bits (453), Expect = 3.404e-53
Identity = 102/259 (39.38%), Postives = 145/259 (55.98%), Query Frame = 3 
Query:  222 QNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAE----CQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISID-SASAECRDLILSCLCRDRENRMDLDKILCH 983
            +N Y +   IG GGFG V+   +IS+   VA+K V++D+I E  +       +P+E  LL +    C+G+     +  +   E    +  +MER     DLFD+I     + EE A     Q++  +  CH   V HRDIKDENIL+D+ T E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF++++EIL   I      S EC+ LI  CL +   +R  L++IL H
Sbjct:   34 ENCYQVGPVIGTGGFGTVYSGVRISDKLPVAIKHVSRDRIGE--MKHMNGTLVPLEIYLLKKVSNGCRGV-----IRLLDWYERPDGFIIIMERPEPVQDLFDFITERGALGEELATNFFRQVVEAVRHCHSCDVVHRDIKDENILVDLRTAELKLIDFGSGALLRDAVYTDFDGTRVYSPPEWIRFHKYHGRSATVWSLGILLYDMVCGDIPFEHDDEILKGKIQYRCRVSRECQHLIEWCLSKRPSDRPSLEQILAH 285
[Close]
BLAST of pim-2 vs. Ensembl Xenopus
Match: ncbp1 (nuclear cap binding protein subunit 1 [Source:Xenbase;Acc:XB-GENE-5841194])

HSP 1 Score: 179.489 bits (454), Expect = 3.705e-53
Identity = 102/259 (39.38%), Postives = 145/259 (55.98%), Query Frame = 3 
Query:  222 QNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAE----CQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISID-SASAECRDLILSCLCRDRENRMDLDKILCH 983
            +N Y +   IG GGFG V+   +IS+   VA+K V++D+I E  +       +P+E  LL +    C+G+     +  +   E    +  +MER     DLFD+I     + EE A     Q++  +  CH   V HRDIKDENIL+D+ T E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF++++EIL   I      S EC+ LI  CL +   +R  L++IL H
Sbjct:   53 ENCYQVGPVIGTGGFGTVYSGVRISDKLPVAIKHVSRDRIGE--MKHMNGTLVPLEIYLLKKVSNGCRGV-----IRLLDWYERPDGFIIIMERPEPVQDLFDFITERGALGEELATNFFRQVVEAVRHCHSCDVVHRDIKDENILVDLRTAELKLIDFGSGALLRDAVYTDFDGTRVYSPPEWIRFHKYHGRSATVWSLGILLYDMVCGDIPFEHDDEILKGKIQYRCRVSRECQHLIEWCLSKRPSDRPSLEQILAH 304
[Close]
BLAST of pim-2 vs. Ensembl Xenopus
Match: PIM3 (Pim-3 proto-oncogene, serine/threonine kinase [Source:NCBI gene;Acc:100497723])

HSP 1 Score: 172.17 bits (435), Expect = 1.899e-50
Identity = 105/275 (38.18%), Postives = 152/275 (55.27%), Query Frame = 3 
Query:  165 MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAEC-QGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCH 983
            M H        VK   +  +  Y +   +G GGFG V+  ++I++G  VA+K VAK++++++         +P+E VLL +   G  GVIKLL+  E      +  VMER     DLFDYI     + E+TA     Q++  +  C+  GV HRDIKDEN+L+D+   E+KLIDFGSG    D  Y DF+GT +YSPPEW++   Y  +    WS+G LLY+M+ GD PF+ + EI+   +      S EC+ LI  CL     +R  L++I  H
Sbjct:   18 MEHLPVKILQPVKVEKEPFEKVYQVGSVLGSGGFGTVYSGSRIADGLPVAVKHVAKERVTDWGTLNGVM--VPLEIVLLKKVGTGFRGVIKLLDWYERA--DGFLIVMERPEPVKDLFDYITEKGALDEDTARGFFRQVLEAVRHCYSCGVVHRDIKDENLLVDLRNGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWVRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEIVRVRLYFRRRISTECQQLIKWCLSLRPSDRPTLEQIFDH 288
[Close]
BLAST of pim-2 vs. Ensembl Xenopus
Match: PASK (PAS domain containing serine/threonine kinase [Source:NCBI gene;Acc:100488299])

HSP 1 Score: 131.339 bits (329), Expect = 1.874e-33
Identity = 90/254 (35.43%), Postives = 135/254 (53.15%), Query Frame = 3 
Query:  228 KYAIYGKIGEGGFGKVFKSTKISNGKS-VALKFVAKDKISEYCLHEETQ-QKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISID-SASAECRDLILSCLCRDRENRMDLDKIL 977
            KY     +G+G FG V+ +    + K  V +KF+ KD++ + C  ++ +  K+  E  +L+  Q    +  +        FQ+   VME + + +DLFD+I+    + E  A +I  QL+  +   H   + HRDIKDENI+I  D   IKL+DFGS  + H G  F  F GT+ Y  PE +    Y   ELE WS+G  LY +I G+ PF   +EIL A ++   + S + + LI   L RD E RM LD++L
Sbjct:  237 KYQTVSPLGKGAFGFVWSACGRDSAKEEVVVKFIRKDRVLDDCWVQDPELGKVTQEIAILSRVQHPNIIRVVDVFENDTFFQL---VMELHGDGLDLFDFIDSQPSLDEPLASYIFRQLVSAVGYLHNQHILHRDIKDENIIIAPDFS-IKLVDFGSAAHLHQGTLFSTFCGTTEYCAPEVLLGNPYPGPELEMWSLGVTLYTLIFGENPFCEVDEILEAELNPPFNVSQKLQVLISGLLQRDPEIRMTLDELL 486
[Close]
BLAST of pim-2 vs. Ensembl Xenopus
Match: PASK (PAS domain containing serine/threonine kinase [Source:NCBI gene;Acc:100488299])

HSP 1 Score: 132.494 bits (332), Expect = 2.005e-33
Identity = 90/254 (35.43%), Postives = 136/254 (53.54%), Query Frame = 3 
Query:  228 KYAIYGKIGEGGFGKVFKST-KISNGKSVALKFVAKDKISEYCLHEETQ-QKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISID-SASAECRDLILSCLCRDRENRMDLDKIL 977
            KY     +G+G FG V+ +  + S  + V +KF+ KD++ + C  ++ +  K+  E  +L+  Q    +  +        FQ+   VME + + +DLFD+I+    + E  A +I  QL+  +   H   + HRDIKDENI+I  D   IKL+DFGS  + H G  F  F GT+ Y  PE +    Y   ELE WS+G  LY +I G+ PF   +EIL A ++   + S + + LI   L RD E RM LD++L
Sbjct:  888 KYQTVSPLGKGAFGFVWSACGRDSAKEEVVVKFIRKDRVLDDCWVQDPELGKVTQEIAILSRVQHPNIIRVVDVFENDTFFQL---VMELHGDGLDLFDFIDSQPSLDEPLASYIFRQLVSAVGYLHNQHILHRDIKDENIIIAPDF-SIKLVDFGSAAHLHQGTLFSTFCGTTEYCAPEVLLGNPYPGPELEMWSLGVTLYTLIFGENPFCEVDEILEAELNPPFNVSQKLQVLISGLLQRDPEIRMTLDELL 1137
[Close]
BLAST of pim-2 vs. Ensembl Mouse
Match: Pim1 (proviral integration site 1 [Source:MGI Symbol;Acc:MGI:97584])

HSP 1 Score: 174.096 bits (440), Expect = 2.288e-51
Identity = 103/273 (37.73%), Postives = 153/273 (56.04%), Query Frame = 3 
Query:  183 SKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQG-IPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            + KL      + ++++Y +   +G GGFG V+   ++++   VA+K V KD+IS++        ++PME VLL +      GVI+LL+  E      +  ++ER     DLFD+I     + E+ A     Q++  +  CH  GV HRDIKDENILID+   EIKLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+++ EI+   +    + S+EC+ LI  CL     +R   ++I  H   Q
Sbjct:   22 ATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVADNLPVAIKHVEKDRISDWG-ELPNGTRVPMEVVLLKKVSSDFSGVIRLLDWFERP--DSFVLILERPEPVQDLFDFITERGALQEDLARGFFWQVLEAVRHCHNCGVLHRDIKDENILIDLSRGEIKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIKGQVFFRQTVSSECQHLIKWCLSLRPSDRPSFEEIRNHPWMQ 291
[Close]
BLAST of pim-2 vs. Ensembl Mouse
Match: Pim3 (proviral integration site 3 [Source:MGI Symbol;Acc:MGI:1355297])

HSP 1 Score: 171.4 bits (433), Expect = 2.618e-50
Identity = 96/264 (36.36%), Postives = 143/264 (54.17%), Query Frame = 3 
Query:  198 VKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXX-XXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEI-LLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCH 983
             K+  +  +  Y +   +G GGFG V+  ++I++G  VA+K V K++++E+         +P+E VLL +     G   ++ +++  E    +  V+ER     DLFD+I     + E  A     Q++  +  CH  GV HRDIKDEN+L+D+ + E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+ + EI           S EC+ LI  CL      R  LD+I  H
Sbjct:   29 AKADKESFEKVYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVVKERVTEW--GSLGGVAVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHNCGVVHRDIKDENLLVDLRSGELKLIDFGSGAVLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLFFRRRVSPECQQLIEWCLSLRPSERPSLDQIAAH 290
[Close]
BLAST of pim-2 vs. Ensembl Mouse
Match: Pim2 (proviral integration site 2 [Source:MGI Symbol;Acc:MGI:97587])

HSP 1 Score: 171.014 bits (432), Expect = 1.334e-49
Identity = 102/263 (38.78%), Postives = 147/263 (55.89%), Query Frame = 3 
Query:  222 QNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLL---AECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCHKLFQK 998
            + +Y +   +G+GGFG VF   ++++ + VA+K ++++++  +    ++    P+E  LL    E  G PGVI+LL+  E    + +  V+ER     DLFDYI     + E  +    TQ++  +  CH  GV HRDIKDENILID+    IKLIDFGSG   HD  Y DF+GT +YSPPEWI    Y       WS+G LLY+M+ GD PF+ + EIL A +   +  S +C  LI  CL     +R  L++IL     Q 
Sbjct:   88 EAEYRLGPLLGKGGFGTVFAGHRVTDRRQVAIKVISRNRVLGWSTVSDSV-TCPLEVALLWKVGEGNGHPGVIRLLDWFETP--EGFMLVLERPMPAQDLFDYITEKGPLGESCSRSFFTQVVAAVQHCHARGVVHRDIKDENILIDLCRGSIKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWSLGVLLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPCSRPSLEEILLDPWMQS 347
[Close]
BLAST of pim-2 vs. Ensembl Mouse
Match: Pim2 (proviral integration site 2 [Source:MGI Symbol;Acc:MGI:97587])

HSP 1 Score: 171.014 bits (432), Expect = 1.552e-49
Identity = 102/263 (38.78%), Postives = 147/263 (55.89%), Query Frame = 3 
Query:  222 QNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLL---AECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCHKLFQK 998
            + +Y +   +G+GGFG VF   ++++ + VA+K ++++++  +    ++    P+E  LL    E  G PGVI+LL+  E    + +  V+ER     DLFDYI     + E  +    TQ++  +  CH  GV HRDIKDENILID+    IKLIDFGSG   HD  Y DF+GT +YSPPEWI    Y       WS+G LLY+M+ GD PF+ + EIL A +   +  S +C  LI  CL     +R  L++IL     Q 
Sbjct:   94 EAEYRLGPLLGKGGFGTVFAGHRVTDRRQVAIKVISRNRVLGWSTVSDSV-TCPLEVALLWKVGEGNGHPGVIRLLDWFETP--EGFMLVLERPMPAQDLFDYITEKGPLGESCSRSFFTQVVAAVQHCHARGVVHRDIKDENILIDLCRGSIKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWSLGVLLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPCSRPSLEEILLDPWMQS 353
[Close]
BLAST of pim-2 vs. Ensembl Mouse
Match: Pask (PAS domain containing serine/threonine kinase [Source:MGI Symbol;Acc:MGI:2155936])

HSP 1 Score: 119.783 bits (299), Expect = 3.690e-29
Identity = 87/253 (34.39%), Postives = 128/253 (50.59%), Query Frame = 3 
Query:  228 KYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQ-QKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGE-YFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKIL 977
            KY     +G G FG V+ + +    K V +KF+ K+K+ E C  E+ +  ++ +E  +L++      +  L      E FQ+   VME++ + +DLF +I+    + E  A FI  QL+  +   H  G+ HRDIKDENI+I  D   IKLIDFGS  Y   G+ ++ F GT  Y  PE +    Y   ELE WS+G  LY +I  + PF    E + A I      S E   L+   L    E R  L+K++
Sbjct: 1058 KYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQL---VMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFT-IKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLI 1306
[Close]
BLAST of pim-2 vs. UniProt/SwissProt
Match: sp|Q9YHZ5|PIM2_DANRE (Serine/threonine-protein kinase pim-2 OS=Danio rerio OX=7955 GN=pim2 PE=2 SV=2)

HSP 1 Score: 183.726 bits (465), Expect = 2.612e-54
Identity = 103/271 (38.01%), Postives = 153/271 (56.46%), Query Frame = 3 
Query:  192 LVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQG----IPGXXXXXXXXXXXXF--QVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCH 983
            L  K+  ++ + +Y +   +G GGFG V+   +IS+G+ VA+K +++D+I ++         +P E  LL    G    +PG   ++ +++  E   Q +  V E+  +  DLFD+I     + E  A   L Q+I  +  CH  G+ HRDIKDENIL+D  T +IK+IDFGSG    D  Y DF GT +YSPPEWI    Y    L  WS+G LLY+M+ GD PF+ + +I+ A  S +   S +CR LI SCL  +  +R  L++IL H
Sbjct:   17 LKAKNGKEHFEKQYTMGNLLGSGGFGSVYSGHRISDGQKVAIKQISRDRIQQWSKMPGEVNPVPNEIALLQSLGGGSGSVPGHRGIIRMLDWFEIPGQEYLIVFEKPQHCQDLFDFITERGALDESLARRFLKQVIEAVQFCHSKGIVHRDIKDENILVDTRTGDIKVIDFGSGATLKDSMYTDFEGTRVYSPPEWILYHKYHALPLTVWSLGVLLYDMVCGDIPFEQDTDIVKAKPSFNKRISNDCRSLICSCLSYNPGDRPSLEQILQH 287
[Close]
BLAST of pim-2 vs. UniProt/SwissProt
Match: sp|P11309|PIM1_HUMAN (Serine/threonine-protein kinase pim-1 OS=Homo sapiens OX=9606 GN=PIM1 PE=1 SV=4)

HSP 1 Score: 178.718 bits (452), Expect = 2.219e-52
Identity = 105/273 (38.46%), Postives = 154/273 (56.41%), Query Frame = 3 
Query:  183 SKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQ-GIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            + KL      + ++++Y +   +G GGFG V+   ++S+   VA+K V KD+IS++        ++PME VLL +   G  GVI+LL+  E      +  ++ER     DLFD+I     + EE A     Q++  +  CH  GV HRDIKDENILID++  E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+++ EI+   +      S+EC+ LI  CL     +R   ++I  H   Q
Sbjct:   22 ATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWG-ELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERP--DSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQ 291
[Close]
BLAST of pim-2 vs. UniProt/SwissProt
Match: sp|Q95LJ0|PIM1_FELCA (Serine/threonine-protein kinase pim-1 OS=Felis catus OX=9685 GN=PIM1 PE=2 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 4.395e-52
Identity = 104/273 (38.10%), Postives = 154/273 (56.41%), Query Frame = 3 
Query:  183 SKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQ-GIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            + KL      + ++++Y +   +G GGFG V+   ++++   VA+K V KD+IS++        ++PME VLL +   G  GVI+LL+  E      +  ++ER     DLFD+I     + EE A     Q++  +  CH  GV HRDIKDENILID++  E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+++ EI+   +      S+EC+ LI  CL     +R   ++I  H   Q
Sbjct:   22 ATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVADNLPVAIKHVEKDRISDWG-ELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERP--DSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPSFEEIQNHPWMQ 291
[Close]
BLAST of pim-2 vs. UniProt/SwissProt
Match: sp|P26794|PIM1_RAT (Serine/threonine-protein kinase pim-1 OS=Rattus norvegicus OX=10116 GN=Pim1 PE=1 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 5.213e-52
Identity = 104/273 (38.10%), Postives = 154/273 (56.41%), Query Frame = 3 
Query:  183 SKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQ-GIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            + KL      + ++++Y +   +G GGFG V+   ++++   VA+K V KD+IS++        ++PME VLL +   G  GVI+LL+  E      +  ++ER     DLFD+I     + EE A     Q++  +  CH  GV HRDIKDENILID++  E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+++ EI+   +      S+EC+ LI  CL     +R   ++I  H   Q
Sbjct:   22 ANKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVADNLPVAIKHVEKDRISDWG-ELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERP--DSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIVKGQVYFRQRVSSECQHLIRWCLSLRPSDRPSFEEIQNHPWMQ 291
[Close]
BLAST of pim-2 vs. UniProt/SwissProt
Match: sp|Q9N0P9|PIM1_BOVIN (Serine/threonine-protein kinase pim-1 OS=Bos taurus OX=9913 GN=PIM1 PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 8.076e-52
Identity = 104/273 (38.10%), Postives = 154/273 (56.41%), Query Frame = 3 
Query:  183 SKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQ-GIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            + KL      + ++++Y +   +G GGFG V+   ++++   VA+K V KD+IS++        ++PME VLL +   G  GVI+LL+  E      +  ++ER     DLFD+I     + EE A     Q++  +  CH  GV HRDIKDENILID++  E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+++ EI+   +      S+EC+ LI  CL     +R   ++I  H   Q
Sbjct:   22 ATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVADNLPVAIKHVEKDRISDWG-ELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERP--DSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHDCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIVRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQ 291
[Close]
BLAST of pim-2 vs. TrEMBL
Match: A0A2P1ERP6 (PIM-1 OS=Schmidtea mediterranea OX=79327 GN=pim-1 PE=2 SV=1)

HSP 1 Score: 567 bits (1460), Expect = 0.000e+0
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 3 
Query:  165 MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA 1025
            MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGVIKLLEIVELEEFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA
Sbjct:    1 MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGVIKLLEIVELEEFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA 287
[Close]
BLAST of pim-2 vs. TrEMBL
Match: I1ZIJ6 (Pim-2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 417.542 bits (1072), Expect = 4.893e-145
Identity = 213/214 (99.53%), Postives = 213/214 (99.53%), Query Frame = 3 
Query:  384 MEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA 1025
            MEAVLLAECQGIPGVIKLLEIVELEEFQVWC VMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA
Sbjct:    1 MEAVLLAECQGIPGVIKLLEIVELEEFQVWCIVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA 214
[Close]
BLAST of pim-2 vs. TrEMBL
Match: T1DBL3 (Pim-1 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 7.717e-69
Identity = 117/256 (45.70%), Postives = 167/256 (65.23%), Query Frame = 3 
Query:  219 IQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCH 983
             +N Y  Y ++G+GGFG V+K+ +  + K V +KF++  K +E    +    ++P+E VLLA+C+ + GVI LL   +  +   W  VM R   F+DLFDYI+    +SEE A F L QL+  LIGCH++GV HRDIKDENIL+D+DT +I LIDFGSG ++ D +Y  ++GT +YSPPEWI+D  Y   +LESWS+G LL++M+ GD PF N+ +IL   +      S +C+DLI SCL  D  +R+ L +I  H
Sbjct:   32 FRNDYQCYEEVGQGGFGSVYKAVRREDQKKVVVKFISNKKNTEKYYWKSHDLEVPLEVVLLAQCKHVKGVITLLAFYQTTQADGWYLVMNRVERFMDLFDYISCKKYLSEEIAAFFLQQLVTILIGCHQSGVLHRDIKDENILVDLDTHDIYLIDFGSGAFFKDDDYTQYDGTRVYSPPEWIRDHRYNGSKLESWSLGILLFDMVCGDIPFSNDQQILSGHLRFRHVVSGQCKDLIHSCLTHDPCDRVTLFEIARH 287
[Close]
BLAST of pim-2 vs. TrEMBL
Match: I1ZIJ1 (Pim-3 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 2.547e-64
Identity = 117/256 (45.70%), Postives = 166/256 (64.84%), Query Frame = 3 
Query:  222 QNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCHK 986
            Q +Y     IGEGGFGKV K+ + S+ K VA+KF++  KI+ +  + +  +++P+E  L+  C+ + GVIK+ +   ++E + W  VM+R  N IDLFD+I    QISE+ A   + QLI  LI CH  GV HRDIKDENI+ID+ T +I LIDFG+G   HDG Y +F+GT +YSPPEWI+ K Y    LE WS+G LLY+M+ G+ P+ N++ I+   ++     S  C+DLI +CL R+ ENR  L  IL H+
Sbjct:   21 QYEYQSLEIIGEGGFGKVRKAIRRSDKKQVAIKFISNSKITSWHFYSKINKQVPLEIALMCRCRKVDGVIKIFKYFYIKEHKGWYIVMDRFENHIDLFDHITNMGQISEKDASNFMNQLITILINCHNLGVCHRDIKDENIIIDLSTNKIYLIDFGAGGILHDGLYSEFDGTRVYSPPEWIQYKRYHAVPLEIWSLGILLYDMVCGNLPYDNDDGIVNCCLAFRRPISHFCQDLIKNCLERNPENRPSLSNILKHR 276
[Close]
BLAST of pim-2 vs. TrEMBL
Match: A0A0R3SR05 (Protein kinase domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS7553 PE=4 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 3.504e-60
Identity = 122/288 (42.36%), Postives = 176/288 (61.11%), Query Frame = 3 
Query:  162 EMNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNP-NFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA--SAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIG 1016
            E + ++ +  L V   V+  +  Y +  +IG+GGFGKV ++ ++  GKSV +K V  D++  +C  +     +PME +LL +   + GV K+LE+ +L     W  VM  NP N +DLFDYI +   ++E  + FI+ QL+  L+ CH AGV HRD+KDENILID +T +I LIDFGSG   H+  Y DF+GT +YSPPEWI+   YF K  E W++G LL++MI GD PF ++NEIL   +    +  S E R+LI +CL +D E R  L +IL H   + +  SI 
Sbjct:   27 ERSSAELNIDLDVIRDVELFERSYELGEQIGKGGFGKVNEAIQVKTGKSVVIKQVDSDRVPCWCRLDGVM--VPMEVILLKKLSKVDGVSKMLEVYDLNG--TWEIVMAGNPPNTLDLFDYICKRGYLTEGESAFIMYQLVGILLQCHAAGVIHRDLKDENILIDPETFKICLIDFGSGSLLHEAIYTDFDGTRVYSPPEWIETGEYFGKAAEVWTVGILLFDMINGDIPFNSDNEILSGQVRFRRSVVSQEVRNLIYNCLRQDPEERPSLVQILQHSWMKMFKKSIA 310
[Close]
BLAST of pim-2 vs. Ensembl Cavefish
Match: pim1 (serine/threonine-protein kinase pim-1-like [Source:NCBI gene;Acc:103031835])

HSP 1 Score: 189.119 bits (479), Expect = 3.736e-57
Identity = 108/257 (42.02%), Postives = 149/257 (57.98%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQ-GIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            Y +   +G GGFG V+   +I++G  VA+K VAKD+++E+     T  ++PME VLL +   G  GVIK+L+  E      +  VMER  N  DLFD+I     + EE A     Q++  +  CH +GV HRDIKDENILID+ + E+KLIDFGSG    D  Y DF+GT +YSPPEWIK   Y  +    WS+G LLY+M+ GD PF+ + EI+   +      S EC+ LI  CL     +R   + ++ H   Q
Sbjct:   36 YQVGSVLGSGGFGTVYAGLRIADGAPVAIKHVAKDRVTEWGELPITGARVPMEIVLLKKVSTGFRGVIKMLDWYERP--DSFIIVMERPENAKDLFDFITEKGALPEELARSFFRQVLEAVRHCHNSGVVHRDIKDENILIDLRSGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIKYHRYHGRSATVWSLGILLYDMVCGDIPFEQDEEIVRGQVLFRRRISTECQQLIKWCLALRPADRPSFEDLVNHPWMQ 290
[Close]
BLAST of pim-2 vs. Ensembl Cavefish
Match: PIM3 (Pim-3 proto-oncogene, serine/threonine kinase [Source:NCBI gene;Acc:103043334])

HSP 1 Score: 181.8 bits (460), Expect = 1.437e-54
Identity = 103/272 (37.87%), Postives = 150/272 (55.15%), Query Frame = 3 
Query:  198 VKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMS 1010
            VK   +  +  Y +   +G GGFG V+  ++IS+G  VA+K VAKD+++E+         +P+E VLL +  G      +  +   E    W  VMER     DLFD+I     + E TA     Q++  +  C+  GV HRDIKDEN+L+D+ T ++KLIDFGSG +  D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+ + EI+   +      SAEC+ LI  CL     +R  L++I  H   +   +S
Sbjct:   10 VKVEPEPFEKVYQVGSVLGSGGFGTVYAGSRISDGLPVAVKHVAKDRVTEWGSINGVM--VPLEIVLLKKV-GSSFRGVIKLLDWYERPDGWLIVMERPELVKDLFDFITEKGALDEATARGFFRQVLEAVRHCYSCGVVHRDIKDENLLVDLRTGDLKLIDFGSGAFLKDTVYTDFDGTRVYSPPEWIRFHRYHGRSATIWSLGVLLYDMVCGDIPFEQDEEIIRGRLYFRRRISAECQQLIKWCLSLRPSDRPTLEQIFEHPWMRPAEVS 278
[Close]
BLAST of pim-2 vs. Ensembl Cavefish
Match: PIM3 (Pim-3 proto-oncogene, serine/threonine kinase [Source:NCBI gene;Acc:103043334])

HSP 1 Score: 181.8 bits (460), Expect = 2.151e-54
Identity = 108/289 (37.37%), Postives = 157/289 (54.33%), Query Frame = 3 
Query:  150 NSN*EMNHSKFSKKL-VVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMS 1010
            N N  M+H     +L  VK   +  +  Y +   +G GGFG V+  ++IS+G  VA+K VAKD+++E+         +P+E VLL +  G      +  +   E    W  VMER     DLFD+I     + E TA     Q++  +  C+  GV HRDIKDEN+L+D+ T ++KLIDFGSG +  D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+ + EI+   +      SAEC+ LI  CL     +R  L++I  H   +   +S
Sbjct:   14 NPN-SMDHLPVKIQLQPVKVEPEPFEKVYQVGSVLGSGGFGTVYAGSRISDGLPVAVKHVAKDRVTEWGSINGVM--VPLEIVLLKKV-GSSFRGVIKLLDWYERPDGWLIVMERPELVKDLFDFITEKGALDEATARGFFRQVLEAVRHCYSCGVVHRDIKDENLLVDLRTGDLKLIDFGSGAFLKDTVYTDFDGTRVYSPPEWIRFHRYHGRSATIWSLGVLLYDMVCGDIPFEQDEEIIRGRLYFRRRISAECQQLIKWCLSLRPSDRPTLEQIFEHPWMRPAEVS 298
[Close]
BLAST of pim-2 vs. Ensembl Cavefish
Match: pim3 (serine/threonine-protein kinase pim-3-like [Source:NCBI gene;Acc:103024220])

HSP 1 Score: 179.104 bits (453), Expect = 2.465e-53
Identity = 99/263 (37.64%), Postives = 149/263 (56.65%), Query Frame = 3 
Query:  201 KSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCHK 986
            KS  +  +  Y +   +G GGFG V+  +++++G  VA+K VAK++++E+         +P+E +LL +  G      +  I   E    W  VMER     DLFDYI     + E+TA     Q++  +  C+  GV HRDIKDEN+L+D+ T ++KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+++ EI+   +      S +C+ LI  CLC    +R  L++I  H+
Sbjct:   31 KSEREPFEKIYQVGSVLGSGGFGTVYAGSRLADGLPVAIKHVAKERVTEWG--SVNGSLVPLEVLLLKKV-GNSFRGVIKLIDWYERPDGWLIVMERPEMVKDLFDYITEKGALDEDTARGFFRQVLEAVRHCYSCGVVHRDIKDENLLVDLRTGDLKLIDFGSGAVLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEHDEEIMKGSLFFRRRISPDCQQLIKWCLCLRPSDRPTLEQIFEHQ 290
[Close]
BLAST of pim-2 vs. Ensembl Cavefish
Match: ENSAMXT00000041332.1 (serine/threonine-protein kinase pim-2-like [Source:NCBI gene;Acc:111191489])

HSP 1 Score: 166.007 bits (419), Expect = 1.378e-48
Identity = 97/259 (37.45%), Postives = 140/259 (54.05%), Query Frame = 3 
Query:  222 QNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAEC--QGIPGXXXXXXXXXXXXFQV--WCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCH 983
            + +Y     +G GGFG VF       G+ + +K V +DK+ ++    +    +PME  LL +   +   G      I  L+ F+V    +++ER     DLFD+I     + E  A   L Q++  +  CH  GV HRDIKDENIL+D  T ++K+IDFGSG    D  Y DF GT ++SPPEWI  + Y    L  WS+G LLY+M+ GD PF+ + +I+ A  S     S EC+ LI  CL    ++R  L++IL H
Sbjct:   29 EKQYKCGALLGSGGFGSVFA------GQRITIKQVCRDKVQQWARLPDKPSLVPMEIALLLQLGRESPAGPSHRGIIRMLDWFEVPGQGYLIERPQPCKDLFDFITERGVVEEPLAQRFLKQVVEAIQFCHSKGVVHRDIKDENILVDTRTGDLKIIDFGSGALLKDSIYTDFEGTRVHSPPEWIVHQQYEAVPLTVWSLGVLLYDMVCGDIPFEMDEDIVSANTSFTKRTSKECQSLIRWCLSFHSKDRPTLEQILQH 281
[Close]
BLAST of pim-2 vs. Ensembl Sea Lamprey
Match: snrka (SNF related kinase a [Source:ZFIN;Acc:ZDB-GENE-040718-84])

HSP 1 Score: 95.9005 bits (237), Expect = 4.931e-22
Identity = 71/264 (26.89%), Postives = 119/264 (45.08%), Query Frame = 3 
Query:  219 IQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRC-SQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFN-GTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-------SAECRDLILSCLCRDRENRMDLDKILCH 983
            I   Y +   +G G F  V  +  +  G+ VA+K + K K+         QQ++         C  +     ++ + E+ + Q   +++    +  DL+DYI R    + EE A     Q++H +  CH+  V HRD+K EN++       +KL DFG  + +  G     + G+  YS PE +    Y    ++ WS+G +LY ++ G  PF+  N+     + +D         S EC+DLI   L RD   R  L ++  H
Sbjct:   12 IAGLYDLEKTLGRGHFAVVKLARHVFTGEKVAVKVIDKSKLDPMATSHLFQQEV--------RCMKLVQHPNVVRLYEVIDTQTKLYLILELADGGDLYDYIMRHEGGLQEELAKTYFAQVVHAISYCHRLHVVHRDLKPENVVFFEKQGLVKLTDFGFSNLFQPGTKLATSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILYMLVCGHPPFQEANDSETLTMIMDCKYTVPAHISRECKDLIARMLQRDPRRRASLQEMEGH 267
[Close]
BLAST of pim-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006410.1 (pep scaffold:Pmarinus_7.0:GL476370:17405:31068:-1 gene:ENSPMAG00000005688.1 transcript:ENSPMAT00000006410.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.5893 bits (231), Expect = 3.223e-21
Identity = 81/280 (28.93%), Postives = 141/280 (50.36%), Query Frame = 3 
Query:  162 EMNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNEN------EILLAPISID-SASAECRDLILSCLCRDRENRMDLDKIL 977
             +N S+    +     + +I N Y +   IG+G F KV  +  I  G+ VA+K + K +++   L     QK+    V + +C   P ++KL E++E E  +    VME   +  ++FDY+    ++ E+ A     Q++  +  CH+  + HRD+K EN+L+DVD   IK+ DFG  + +      D F G+  Y+ PE  + K Y   E++ WS+G +LY +++G  PF  +N      ++L     I    S +C +L+   L  +   R  L++I+
Sbjct:   17 RVNGSRSRSSIACGEELPHIGN-YRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSL-----QKL-FREVRIMKCLNHPNIVKLYEVIETE--KTLYLVMEYA-SGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKNIVHRDLKAENLLLDVDM-HIKIADFGFSNEFTLVNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELREKVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGSLEQIM 285
[Close]
BLAST of pim-2 vs. Ensembl Sea Lamprey
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897])

HSP 1 Score: 89.3521 bits (220), Expect = 3.904e-20
Identity = 76/257 (29.57%), Postives = 130/257 (50.58%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNEN------EILLAPISID-SASAECRDLILSCLCRDRENRMDLDKIL 977
            Y +   IG+G F KV  +     G+ VA+K + K +++   L     QK+  E V + +    P ++KL E++E+E  +    VME +    ++FDY+    ++ E+ A     Q++  +  CH+  + HRD+K EN+L+D D   IK+ DFG  + +  G   D F G+  Y+ PE  + K Y   E++ WS+G +LY +++G  PF  +N       +L     I    S +C +L+   L      R  L++I+
Sbjct:   68 YRLLKTIGKGNFAKVKLAKHTLTGQEVAVKIIDKTQLNTSSL-----QKLFRE-VRIMKVLNHPNIVKLFEVIEME--KTLYLVMEYSSGG-EVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHIVHRDLKAENLLLDADM-NIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLHPLKRGSLEQIM 314
[Close]
BLAST of pim-2 vs. Ensembl Sea Lamprey
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897])

HSP 1 Score: 88.9669 bits (219), Expect = 9.804e-20
Identity = 75/257 (29.18%), Postives = 129/257 (50.19%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNEN------EILLAPISID-SASAECRDLILSCLCRDRENRMDLDKIL 977
            Y +   IG+G F KV  +     G+ VA+K + K +++   L     QK+    V + +    P ++KL E++E E  +    VME   +  ++FDY+    ++ E+ A     Q++  +  CH+  + HRD+K EN+L+D D   IK+ DFG  + +  G   D F G+  Y+ PE  + K Y   E++ WS+G +LY +++G  PF  +N       +L     I    S +C +L+   L  +   R  L++I+
Sbjct:   54 YRLLKTIGKGNFAKVKLARHTLTGREVAVKIIDKTQLNPSSL-----QKL-FREVRIMKILNHPNIVKLFEVIETE--KTLYLVMEYA-SGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHIVHRDLKAENLLLDADM-NIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPAKRGSLEQIM 300
[Close]
BLAST of pim-2 vs. Ensembl Sea Lamprey
Match: phkg1b (phosphorylase kinase, gamma 1b (muscle) [Source:ZFIN;Acc:ZDB-GENE-050522-52])

HSP 1 Score: 87.0409 bits (214), Expect = 1.947e-19
Identity = 77/279 (27.60%), Postives = 126/279 (45.16%), Query Frame = 3 
Query:  225 NKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFV--AKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCF-VMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYF-DFNGTSIYSPPEWIK---DKHY--FVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-----------SAECRDLILSCLCRDRENRMDLDKILCHKLFQKY 1001
            +KY     +G G    V +      G+  A+K +    D++SE  L E+       E  +L    G P +I L++  E   F    F +M+R     +LFDY+     +SE+    I+  +   +   H   + HRD+K ENIL+D D   I+L DFG       GE   +  GT  Y  PE +K   D+ +  + KE++ W+ G ++Y ++ G  PF +  ++ +  + +D             S   +DLI   L  + E R   ++ L H  FQ+Y
Sbjct:   23 DKYEPKEVLGSGVSSVVRRCVHKRTGQDFAVKIIDITPDRLSEEEL-EDIYSSTVKEVDILKAVSGHPHIITLIDAYESSTFFFLVFDLMKRG----ELFDYLTEKVALSEKDTRSIMRSIFEVVHFLHAKNIVHRDLKPENILLD-DNMNIRLSDFGFSMQLRPGERLRELCGTPGYLSPELLKCSMDESHPGYGKEVDLWACGVIMYTLLAGSPPFWHRKQMTMLRLIMDGKYDFSTPEWEDRSDTVKDLISRLLVVEPEKRFTAEQALAHPFFQQY 295
[Close]
BLAST of pim-2 vs. Ensembl Yeast
Match: PSK2 (PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication [Source:SGD;Acc:S000005405])

HSP 1 Score: 100.523 bits (249), Expect = 9.944e-24
Identity = 59/160 (36.88%), Postives = 86/160 (53.75%), Query Frame = 3 
Query:  504 IDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA---SAECRDLILSCLCRDRENRMDLDKI 974
            IDLFD I     + E  A  +  Q++ ++   H  G+ HRDIKDEN+++D     +KLIDFGS  Y   G +  F GT  Y+ PE +    Y  K  + W++G LLY +I  + P+ N +EIL   +  D +   S EC  LI   L R+ + R  +D+I
Sbjct:  935 IDLFDVIEFKKDMVEHEAKLVFKQVVASIKHLHDQGIVHRDIKDENVIVD-SHGFVKLIDFGSAAYIKSGPFDVFVGTMDYAAPEVLGGSSYKGKPQDIWALGVLLYTIIYKENPYYNIDEILEGELRFDKSEHVSEECISLIKRILTREVDKRPTIDEI 1093
[Close]
BLAST of pim-2 vs. Ensembl Yeast
Match: PSK1 (PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication [Source:SGD;Acc:S000000015])

HSP 1 Score: 98.9821 bits (245), Expect = 4.198e-23
Identity = 79/252 (31.35%), Postives = 122/252 (48.41%), Query Frame = 3 
Query:  246 KIGEGGFGKVFKSTKISNGKSVALKFVAKDKI-SEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNP-----NFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA---SAECRDLILSCLCRDRENRMDLDKI 974
            K+GEG +GKV       N   V +K + K++I  +  + +     IP E  ++A     P       +  L+ F+   +     P       IDLFD I   + ++E  A  I  Q++  +   H  G+ HRDIKDEN+++D     +K+IDFGS  Y   G +  F GT  Y+ PE +    Y  +  + W+IG LLY ++  + PF N +EIL   +  ++A   S +C +LI S L R    R  +D I
Sbjct: 1101 KMGEGAYGKVNLCIHKKNRYIVVIKMIFKERILVDTWVRDRKLGTIPSEIQIMATLNKKP---HENILRLLDFFEDDDYYYIETPVHGETGCIDLFDLIEFKTNMTEFEAKLIFKQVVAGIKHLHDQGIVHRDIKDENVIVD-SKGFVKIIDFGSAAYVKSGPFDVFVGTIDYAAPEVLGGNPYEGQPQDIWAIGILLYTVVFKENPFYNIDEILEGDLKFNNAEEVSEDCIELIKSILNRCVPKRPTIDDI 1348
[Close]
BLAST of pim-2 vs. Ensembl Yeast
Match: PKH1 (Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication [Source:SGD;Acc:S000002898])

HSP 1 Score: 97.8265 bits (242), Expect = 7.020e-23
Identity = 83/282 (29.43%), Postives = 133/282 (47.16%), Query Frame = 3 
Query:  246 KIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQ---VWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYY-------HDGE-YFD-------FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILL--APISIDSASAE-----CRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIG 1016
            ++G+G +  V  +T   +GK  A+K ++K    EY + ++  + + +E + L +  G  G+ KL        FQ      F++E  P+  D    I +   ++E  A +  +Q+I  +   H  G+ HRDIK ENIL+D + K +KL DFG+            DG+ YFD       F GT+ Y  PE + D +Y     + W+ G +LY M+ G  PFK  NE L     + I  A         +DL+   L RD  +R+ + +I  H  F + +   G
Sbjct:  130 QLGDGSYSSVVLATARDSGKKYAVKVLSK----EYLIRQKKVKYVTVEKLALQKLNGTKGIFKLFFT-----FQDEASLYFLLEYAPHG-DFLGLIKKYGSLNETCARYYASQIIDAVDSLHNIGIIHRDIKPENILLDKNMK-VKLTDFGTAKILPEEPSNTADGKPYFDLYAKSKSFVGTAEYVSPELLND-NYTDSRCDIWAFGCILYQMLAGKPPFKAANEYLTFQKVMKIQYAFTAGFPQIVKDLVKKLLVRDPNDRLTIKQIKAHLFFHEVNFEDG 399
[Close]
BLAST of pim-2 vs. Ensembl Yeast
Match: HSL1 (Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; autophosphorylation enhances interactions with Hsl7p [Source:SGD;Acc:S000001584])

HSP 1 Score: 91.6633 bits (226), Expect = 1.000e-20
Identity = 55/173 (31.79%), Postives = 89/173 (51.45%), Query Frame = 3 
Query:  507 DLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFN-GTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNEN--EILLAPIS-----IDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKY 1001
            +LFDY+    ++ E  A+    Q++  +  CH   + HRD+K EN+L+D   + IK+ DFG        +    + G+  Y+ PE +  + Y     + WS G +L+ ++TG  PF ++N  ++LL   S       + S+E RDLI   L  D E R+   +IL H L +KY
Sbjct:  200 ELFDYLVSKGKLPEREAIHYFKQIVEGVSYCHSFNICHRDLKPENLLLDKKNRRIKIADFGMAALELPNKLLKTSCGSPHYASPEIVMGRPYHGGPSDVWSCGIVLFALLTGHLPFNDDNIKKLLLKVQSGKYQMPSNLSSEARDLISKILVIDPEKRITTQEILKHPLIKKY 372
[Close]
BLAST of pim-2 vs. Ensembl Yeast
Match: SNF1 (AMP-activated S/T protein kinase; forms a complex with Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; regulates nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth and acts as a non-canonical GEF, activating Arf3p during invasive growth; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ub ligase [Source:SGD;Acc:S000002885])

HSP 1 Score: 88.1965 bits (217), Expect = 9.788e-20
Identity = 66/212 (31.13%), Postives = 107/212 (50.47%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFN-GTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNEN 863
            Y I   +GEG FGKV  +   + G+ VALK + K  +++  +    +++I     LL     I     +    E+        V+E   N  +LFDYI +  ++SE+ A     Q+I  +  CH+  + HRD+K EN+L+D +   +K+ DFG  +   DG +   + G+  Y+ PE I  K Y   E++ WS G +LY M+    PF +E+
Sbjct:   55 YQIVKTLGEGSFGKVKLAYHTTTGQKVALKIINKKVLAKSDMQGRIEREISY-LRLLRHPHIIKLYDVIKSKDEI------IMVIEYAGN--ELFDYIVQRDKMSEQEARRFFQQIISAVEYCHRHKIVHRDLKPENLLLD-EHLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYVMLCRRLPFDDES 256
[Close]
BLAST of pim-2 vs. Ensembl Nematostella
Match: EDO46861 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNH5])

HSP 1 Score: 153.295 bits (386), Expect = 2.316e-44
Identity = 89/238 (37.39%), Postives = 131/238 (55.04%), Query Frame = 3 
Query:  273 VFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCH 983
            V+  T++ +GK VA+K + K+ +  Y   E     +PME V L      P V++L +  E    Q +  V+ER  ++ DLFDYI     +SE  A  I  Q++H +  C   GV HRDIKDEN+L+D  T ++KL DFGSG    +  Y D+ GT  Y PPEW +   Y  +    WS+G LL+NM++GD PF N+ EI+   ++  ++ S E + L+   L +    R  L+ IL H
Sbjct:    2 VYAGTRVRDGKPVAIKVIPKNNV--YFFEEVGGISVPME-VYLHRVLDHPNVVRLYDYFEYN--QAYVLVLERPTHYRDLFDYITEKKLLSEREAKDIFKQVVHAVAYCEGKGVFHRDIKDENLLLDTKTGQVKLFDFGSGTVLENTLYTDYEGTRAYCPPEWFRFHRYHARPCTVWSLGILLFNMLSGDVPFANQIEIVRGELNFAENVSKEAQSLVRWLLAKHPSYRPTLENILKH 234
[Close]
BLAST of pim-2 vs. Ensembl Nematostella
Match: EDO30548 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0G6])

HSP 1 Score: 99.7525 bits (247), Expect = 4.134e-25
Identity = 66/180 (36.67%), Postives = 95/180 (52.78%), Query Frame = 3 
Query:  228 KYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWI 764
            KY     IG+G FG V  + + ++   V +KF+ K KI   C  E     +P+E  LLA+ +    V  L      E FQV   VME++   +DLF++I+R   + E    +I  QL+  +   H   + HRD+KDENI++D +   IKLIDFGS  Y  +G+ F  F GT  Y  PE +
Sbjct:    8 KYLTLKSIGKGAFGFVQMARRRADMSEVVVKFIRKAKILPDCWVEGPMGNVPLEIALLAKLKHPNIVKMLDAFENEEFFQV---VMEKHGTGMDLFEFIDRQPALDEPLCAYIFRQLVSAISFLHNQNILHRDVKDENIILD-EKFNIKLIDFGSAAYMEEGKVFSTFCGTMEYCSPEVL 183
[Close]
BLAST of pim-2 vs. Ensembl Nematostella
Match: EDO30768 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZV3])

HSP 1 Score: 96.6709 bits (239), Expect = 4.881e-22
Identity = 72/261 (27.59%), Postives = 126/261 (48.28%), Query Frame = 3 
Query:  228 KYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNEN------EILLAPISID-SASAECRDLILSCLCRDRENRMDLDKILCHK 986
            +Y +   IG+G F KV  +  +  GK VA+K + K +++   L     QK+  E  ++   + +     +     +E  +    VME      ++FDY+    ++ E+ A     Q++  +  CH+  V HRD+K EN+L+D D   IK+ DFG  + +  G   D F G+  Y+ PE  + K Y   E++ WS+G +LY +++G  PF  +N       +L     I    S +C +L+   L  + + R  L++ +  K
Sbjct:   37 RYRLIKTIGKGNFAKVKLAKHVPTGKEVAIKIIDKTQLNPSSL-----QKLFREVRIM---KFLDHPNIVKLYEVIETDKTLYLVMEYASGG-EVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHVIHRDLKAENLLLDADM-NIKIADFGFSNEFTPGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVLNPQKRARLEQTMTDK 287
[Close]
BLAST of pim-2 vs. Ensembl Nematostella
Match: EDO36587 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI81])

HSP 1 Score: 93.2041 bits (230), Expect = 1.637e-21
Identity = 70/269 (26.02%), Postives = 121/269 (44.98%), Query Frame = 3 
Query:  219 IQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYI-NRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFN-GTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFK--NENEILLAPISI-----DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQK 998
            I   Y +   +G G F  V  +  +  G+ VA+K + K K+ E       ++           C  +     ++ + ++ +     +++    +  D++DYI N    + EE A +   Q++  +  CHK  V HRD+K EN++   +    KL DFG  + +   E  D   G+  YS PE +    Y    ++ WS+G +LY ++ G  PF   N++E L   +       D  S  C++LI   L R+  NR  L +I+ H   Q+
Sbjct:   15 IAGMYDLGETLGRGHFAVVKVARHVITGERVAVKVIDKTKLDEVSREHLLKE---------VRCMKLVQHPNVVRLYQVIDTNTKLYLILELGDGGDMYDYIMNHDKGLPEEKARYFFRQIVLAIDYCHKLHVVHRDLKPENVIFFKNQDMAKLTDFGFSNNFIPNEKLDTACGSLAYSAPEVLLGDAYEAPAVDVWSLGVILYMLVCGQAPFSEANDSETLTKIMDCRYDVPDHVSPLCKNLISRMLIREPHNRASLGEIMSHPWLQQ 274
[Close]
BLAST of pim-2 vs. Ensembl Nematostella
Match: EDO37143 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGS0])

HSP 1 Score: 91.2781 bits (225), Expect = 3.935e-21
Identity = 78/262 (29.77%), Postives = 127/262 (48.47%), Query Frame = 3 
Query:  243 GKIGEGGFGKV--FKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVW--CFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNEN-EILLAPISI--------DSASAECRDLILSCLCRDRENRMDLDKILCHK 986
            G++GEG + KV    ST+++N   VA+K + K +     ++    + +P E V++   +          I  L+ F+V    +V+       DL +YI     + E  A  + +QL+  +  CH+ GV HRD+K EN+L+D+    +KL DFG        E    F G++ Y+  E ++   Y  ++ + WS+G +LY M+TG  PF + N   LL  I             S ECRDLI   L  + E R+ + +I  H+
Sbjct:   22 GRLGEGTYAKVKCAYSTQLAN--HVAVKIIDKKRAPSDFIN----KFLPREMVVIKRLR------HKHIIQVLDLFEVREKVYVIMELATRGDLLEYIRYRGCVRERKAKKVFSQLLQAVKYCHQQGVIHRDLKCENVLLDIGD-NVKLTDFGFSKLNPRKELCKTFCGSAAYAAIEILQGTEYDGEKADIWSLGIILYTMVTGRMPFDDANMTTLLRQIKRGVEFRKPKQMVSEECRDLIRCMLTHNYEYRITIPEIEAHR 270
[Close]
BLAST of pim-2 vs. Ensembl Medaka
Match: pim1 (serine/threonine-protein kinase pim-1 [Source:NCBI gene;Acc:101157054])

HSP 1 Score: 187.963 bits (476), Expect = 9.742e-57
Identity = 107/259 (41.31%), Postives = 146/259 (56.37%), Query Frame = 3 
Query:  231 YAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQ---VWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            Y +   +G GGFG V+  T+IS+G  VA+K VA+D++SE+     +  ++PME VLL +     G      I  L+ F+    +  VMER  N  DLFD+I     + EE A     Q++  +  CH  GV HRDIKDENILID+ T E+KLIDFGSG    D  Y DF+GT +YSPPEWIK   Y  +    WS+G LLY+M+ GD PF+++ EI    +      S EC+ LI  CL     +R   + ++ H   Q
Sbjct:   34 YQVGAVLGSGGFGTVYAGTRISDGVPVAVKHVARDRVSEWG-ELPSGARVPMEIVLLKKV----GTGFRGVIKMLDWFERPDSFIIVMERPENVKDLFDFITERGALPEELARSFFRQVLEAVRHCHSCGVVHRDIKDENILIDLRTGEMKLIDFGSGALLKDTIYTDFDGTRVYSPPEWIKYHRYQGRSATVWSLGILLYDMVCGDIPFEHDEEITRGQVLFRRRISTECQHLIKWCLSLRPTDRPSFEDVINHSWMQ 287
[Close]
BLAST of pim-2 vs. Ensembl Medaka
Match: pim3 (Pim-3 proto-oncogene, serine/threonine kinase [Source:NCBI gene;Acc:101171374])

HSP 1 Score: 176.792 bits (447), Expect = 2.062e-52
Identity = 102/278 (36.69%), Postives = 147/278 (52.88%), Query Frame = 3 
Query:  165 MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQ 995
            M+H        VK   +  +  Y +   +G GGFG V+   +IS+G  VA+K VAK++++E+         +P+E +LL +         +  +   E    W  VMER     DLFD+I     + E TA     Q++  +  C+  GV HRDIKDEN+L+D+ T E+KLIDFGSG    D  Y DF+GT +YSPPEWI+   Y  +    WS+G LLY+M+ GD PF+ + EIL   +      S EC+ +I  CL     +R  LD+I  H   +
Sbjct:   18 MDHIPVKILPPVKVEKEPFERVYQVGSVLGSGGFGTVYAGNRISDGAPVAVKHVAKERVTEWGSINGVM--VPLEILLLKKVSS-SFRGVIKLLDYYERPDGWLIVMERPDLVKDLFDFITEKGALDEGTARGFFRQVLEAVRHCYSCGVVHRDIKDENLLVDLRTGELKLIDFGSGAILKDTVYTDFDGTRVYSPPEWIRFHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGKVYFRRRVSPECQQIIKWCLALRPSDRPTLDQICDHPWMR 292
[Close]
BLAST of pim-2 vs. Ensembl Medaka
Match: ENSORLT00000032540.1 (serine/threonine-protein kinase pim-2-like [Source:NCBI gene;Acc:101156739])

HSP 1 Score: 166.392 bits (420), Expect = 2.574e-48
Identity = 103/276 (37.32%), Postives = 150/276 (54.35%), Query Frame = 3 
Query:  183 SKKLVVKSTVKYIQNKYAIYG-KIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQ-VWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISI-DSASAECRDLILSCLCRDRENRMDLDKILCHKLFQK 998
            ++K +  S +K  + KY +   ++G+GGFGKVF   + S+  SVALKFV    +   C   +   K P+E V L      P    ++ +++  + +  W  V E   N  DLF YI  C ++ E+TA   L Q+I  L+ CH  GV H D+KDEN+L+D  T ++ LIDFGS  +    + +D F+GT++Y+PPE +    Y     E WS+G LLY+M+ GD PF+N+ EILL  +      S +C  L+  CL R  E R  L  IL H    K
Sbjct:    5 TQKTIDPSAMKTFREKYWMSSHRLGKGGFGKVFLGKRRSDRISVALKFVPL--LRTRCCEVDGTSK-PLEVVALEMVTKDPPFPGIIRMLDFYKTRGAWVIVTETPENTQDLFHYITTCKRLCEDTARDFLGQVIQALLHCHSRGVVHMDVKDENLLLDPSTHKLLLIDFGSAVFLEPDKVYDTFHGTAVYAPPERLTQGCYKALPSEVWSLGILLYDMVHGDIPFQNDEEILLGELRFKKDLSEDCVALMQWCLSRAPEERPTLKDILAHPWMSK 277
[Close]
BLAST of pim-2 vs. Ensembl Medaka
Match: ENSORLT00000010626.2 (pep primary_assembly:ASM223467v1:12:12000679:12006348:1 gene:ENSORLG00000008461.2 transcript:ENSORLT00000010626.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 162.54 bits (410), Expect = 5.928e-47
Identity = 104/288 (36.11%), Postives = 150/288 (52.08%), Query Frame = 3 
Query:  144 EINSN*EMNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQ-VWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCHKLFQK 998
            E+ +       + +  L V+S   +   KY     +G+GGFGKVF   + S+  SVALKFV    +   C   +   K P+E V L      P    ++ +++  + +  W  V E   N  DLF YI  C ++ E+TA   L Q+I  L+ CH  GV H D+KDEN+L+D  T ++ LIDFGS  +    + +D F+GT++Y+PPE +    Y     E WS+G LLY+M+ GD PF+N+ EILL  +      S +C  L+  CL R  E R  L  IL H    K
Sbjct:   22 EVQTQDLPQRGRMALPLKVQSVTVF---KYVRLHCLGKGGFGKVFLGKRRSDRISVALKFVPL--LRTRCCEVDGTSK-PLEVVALEMVTKDPPFPGIIRMLDFYKTRGAWVIVTETPENTQDLFHYITTCKRLCEDTARDFLGQVIQALLHCHSRGVVHMDVKDENLLLDPSTHKLLLIDFGSAVFLEPDKVYDTFHGTAVYAPPERLTQGCYKALPSEVWSLGILLYDMVHGDIPFQNDEEILLGELRFKKDLSEDCVALMQWCLSRAPEERPTLKDILAHPWMSK 303
[Close]
BLAST of pim-2 vs. Ensembl Medaka
Match: ENSORLT00000041570.1 (pep primary_assembly:ASM223467v1:10:18938984:18940525:-1 gene:ENSORLG00000023590.1 transcript:ENSORLT00000041570.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 159.844 bits (403), Expect = 4.281e-45
Identity = 98/253 (38.74%), Postives = 138/253 (54.55%), Query Frame = 3 
Query:  249 IGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQV-WCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFD-FNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCHKLFQK 998
            +G+GGFGKVF   + S+G SVALK +   +    C   +   K P EAV L      P    ++ +++  +    W  V E+  N  DL++YI    ++ E+TA   L Q+I  L+ CH  GV H D+KDEN+L+D+ T E+KLIDFGS  +    + +D F+GT+ Y PPE +    Y     E WS+G LLY+M+ GD PF N+ E+LL  +  +   S +C  LI  CL R  E R  L  IL H    K
Sbjct:   47 LGKGGFGKVFLGKRRSDGISVALKIIPLSRTR--CCEVDGTSK-PFEAVALEMVSKDPPFPGIIRMLDFYKTPYNWVIVTEKPENTQDLYEYITISKRLREDTAREFLGQVIQALLHCHSRGVVHMDVKDENLLVDLTTLELKLIDFGSAEFLEPDKVYDMFHGTAAYGPPERLTQGCYKALPSEVWSLGILLYDMVHGDIPFGNDRELLLGELRFEKDLSEDCVALIKWCLSRTPEERPMLKDILAHPWMLK 296
[Close]
BLAST of pim-2 vs. Planmine SMEST
Match: SMESG000027798.1 (SMESG000027798.1)

HSP 1 Score: 564.303 bits (1453), Expect = 0.000e+0
Identity = 286/287 (99.65%), Postives = 286/287 (99.65%), Query Frame = 3 
Query:  165 MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA 1025
            MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGVIKLLEIVELEEFQVWC VMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA
Sbjct:    1 MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGVIKLLEIVELEEFQVWCIVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA 287
[Close]
BLAST of pim-2 vs. Planmine SMEST
Match: SMESG000014593.1 (SMESG000014593.1)

HSP 1 Score: 218.779 bits (556), Expect = 6.965e-68
Identity = 117/256 (45.70%), Postives = 166/256 (64.84%), Query Frame = 3 
Query:  222 QNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCHK 986
            Q +Y     IGEGGFGKV K+ + S+ K VA+KF++  KI+ +  + +  +++P+E  L+  C+ + GVIK+ +   ++E + W  VM+R  N IDLFD+I    QISE+ A   + QLI  LI CH  GV HRDIKDENI+ID+ T +I LIDFG+G   HDG Y +F+GT +YSPPEWI+ K Y    LE WS+G LLY+M+ G+ P+ N++ I+   ++     S  C+DLI +CL R+ ENR  L  IL H+
Sbjct:   21 QYEYQSLEIIGEGGFGKVRKAIRRSDKKQVAIKFISNSKITSWHFYSKINKQVPLEIALMCRCRKVDGVIKIFKYFYIKEHKGWYIVMDRFENHIDLFDHITNMGQISEKDASNFMNQLITILINCHNLGVCHRDIKDENIIIDLSTNKIYLIDFGAGGILHDGLYSEFDGTRVYSPPEWIQYKRYHAVPLEIWSLGILLYDMVCGNLPYDNDDGIVNCCLAFRRPISHFCQDLIKNCLERNPENRPSLSNILKHR 276
[Close]
BLAST of pim-2 vs. Planmine SMEST
Match: SMESG000017137.1 (SMESG000017137.1)

HSP 1 Score: 218.779 bits (556), Expect = 1.183e-67
Identity = 113/267 (42.32%), Postives = 169/267 (63.30%), Query Frame = 3 
Query:  213 KYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMS 1010
            ++ ++ Y  + +IG+GGFG VFK T++S+   V +KF++  KI++    +     IP+E  LLA+C+ + GVI +L   + +  + W  +M R   F+DLFDYI+R   +SEE A F   Q+I  L  CH+  V HRDIKDENIL+D +T  + LIDFGSG +    +Y +F+GT +YSPPEWIK+  Y   +LE WS+G LL++M+ GD PF  +++IL   +      SA+CRDLI  CL  D   R++L++I  H   QK+H++
Sbjct:   26 EFFRSHYQCHEEIGQGGFGTVFKGTRLSDRLEVIIKFISNKKITDTYKWKAKNLAIPLEVALLAKCRPVKGVIDMLAFYKSDGKKGWYLIMNRIAKFVDLFDYISRAKYLSEEKAAFFFRQIIEILKQCHQQKVLHRDIKDENILVDENTDFVYLIDFGSGAFLKTDDYRNFDGTRVYSPPEWIKNHCYNGFKLEVWSLGVLLFDMVCGDIPFNTDDQILNGKLKYRHCVSADCRDLIEKCLKHDPSYRINLNEISNHPWVQKHHLN 292
[Close]
BLAST of pim-2 vs. Planmine SMEST
Match: SMESG000017137.1 (SMESG000017137.1)

HSP 1 Score: 218.394 bits (555), Expect = 1.212e-67
Identity = 113/267 (42.32%), Postives = 169/267 (63.30%), Query Frame = 3 
Query:  213 KYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDS-ASAECRDLILSCLCRDRENRMDLDKILCHKLFQKYHMS 1010
            ++ ++ Y  + +IG+GGFG VFK T++S+   V +KF++  KI++    +     IP+E  LLA+C+ + GVI +L   + +  + W  +M R   F+DLFDYI+R   +SEE A F   Q+I  L  CH+  V HRDIKDENIL+D +T  + LIDFGSG +    +Y +F+GT +YSPPEWIK+  Y   +LE WS+G LL++M+ GD PF  +++IL   +      SA+CRDLI  CL  D   R++L++I  H   QK+H++
Sbjct:   22 EFFRSHYQCHEEIGQGGFGTVFKGTRLSDRLEVIIKFISNKKITDTYKWKAKNLAIPLEVALLAKCRPVKGVIDMLAFYKSDGKKGWYLIMNRIAKFVDLFDYISRAKYLSEEKAAFFFRQIIEILKQCHQQKVLHRDIKDENILVDENTDFVYLIDFGSGAFLKTDDYRNFDGTRVYSPPEWIKNHCYNGFKLEVWSLGVLLFDMVCGDIPFNTDDQILNGKLKYRHCVSADCRDLIEKCLKHDPSYRINLNEISNHPWVQKHHLN 288
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BLAST of pim-2 vs. Planmine SMEST
Match: SMESG000072329.1 (SMESG000072329.1)

HSP 1 Score: 181.8 bits (460), Expect = 2.888e-53
Identity = 109/275 (39.64%), Postives = 159/275 (57.82%), Query Frame = 3 
Query:  168 NHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVALKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGXXXXXXXXXXXXFQVWCFVMERNPNFIDLFDYINRCSQ--ISEETAVFILTQLIHTLIGCHKAGVAHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWIKDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSA-SAECRDLILSCLCRDRENRMDLDKILCH 983
            ++  + +K   +     ++ KY    ++G+G +G V    +  +  SVA+K +  + I  +   +  +  IPME +LL+    I GVI ++E     E   W  VMER    IDLF+YI  CS+  ++E  A  I  +L   +I CH  G+ HRDIK+ENILID  TK+I LIDFGSG   +D  ++ +NGT  Y+PPEWIK  HY   +LESWS+G +LY+M+ G  PF++ NEIL   I      S  C++LIL C+ RD +NR+   +IL H
Sbjct:    4 DYENYLRKFPFQQGKTKLELKYNYDKELGKGSYGNVLSGFRKKDHFSVAIKVLPTNLIDNWYFSKTLKMNIPMEILLLSLASNIDGVISMIEYYNDLEQSEWYIVMERFAETIDLFNYI--CSEGFLTEHLASQIFYELTRIIIECHLRGILHRDIKEENILIDKYTKKIWLIDFGSGGLMNDDNHYSYNGTEEYAPPEWIKYGHYRGCQLESWSLGIVLYSMVCGRIPFEDNNEILSNTIKFPRKISNNCKELILKCIERDPKNRIQPSEILKH 276
The following BLAST results are available for this feature:
BLAST of pim-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PIM14.621e-5338.46Pim-1 proto-oncogene, serine/threonine kinase [Sou... [more]
PIM35.860e-5136.36Pim-3 proto-oncogene, serine/threonine kinase [Sou... [more]
PIM23.012e-4637.79Pim-2 proto-oncogene, serine/threonine kinase [Sou... [more]
PIM22.742e-3648.51Pim-2 proto-oncogene, serine/threonine kinase [Sou... [more]
PASK1.150e-2833.20PAS domain containing serine/threonine kinase [Sou... [more]
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BLAST of pim-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prk-22.221e-5038.11Serine/threonine-protein kinase prk-2 [Source:Uni... [more]
prk-22.221e-5038.11Serine/threonine-protein kinase prk-2 [Source:Uni... [more]
prk-19.532e-4937.88Pim (Mammalian oncogene) Related Kinase [Source:U... [more]
prk-14.477e-4737.88Pim (Mammalian oncogene) Related Kinase [Source:U... [more]
prk-15.248e-4737.88Pim (Mammalian oncogene) Related Kinase [Source:U... [more]
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BLAST of pim-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pask2.217e-2631.00gene:FBgn0034950 transcript:FBtr0072160[more]
Sik39.653e-2328.57gene:FBgn0262103 transcript:FBtr0344259[more]
Sik39.657e-2328.57gene:FBgn0262103 transcript:FBtr0344258[more]
Sik39.783e-2328.57gene:FBgn0262103 transcript:FBtr0340388[more]
Sik39.788e-2328.57gene:FBgn0262103 transcript:FBtr0304026[more]
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BLAST of pim-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pim24.820e-5538.01Pim-2 proto-oncogene, serine/threonine kinase [Sou... [more]
pim13.279e-5441.63Pim-1 proto-oncogene, serine/threonine kinase [Sou... [more]
pim31.954e-5237.97Pim-3 proto-oncogene, serine/threonine kinase [Sou... [more]
pimr251.969e-4132.38Pim proto-oncogene, serine/threonine kinase, relat... [more]
pimr242.097e-4132.38Pim proto-oncogene, serine/threonine kinase, relat... [more]
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BLAST of pim-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ncbp13.404e-5339.38nuclear cap binding protein subunit 1 [Source:Xenb... [more]
ncbp13.705e-5339.38nuclear cap binding protein subunit 1 [Source:Xenb... [more]
PIM31.899e-5038.18Pim-3 proto-oncogene, serine/threonine kinase [Sou... [more]
PASK1.874e-3335.43PAS domain containing serine/threonine kinase [Sou... [more]
PASK2.005e-3335.43PAS domain containing serine/threonine kinase [Sou... [more]
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BLAST of pim-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pim12.288e-5137.73proviral integration site 1 [Source:MGI Symbol;Acc... [more]
Pim32.618e-5036.36proviral integration site 3 [Source:MGI Symbol;Acc... [more]
Pim21.334e-4938.78proviral integration site 2 [Source:MGI Symbol;Acc... [more]
Pim21.552e-4938.78proviral integration site 2 [Source:MGI Symbol;Acc... [more]
Pask3.690e-2934.39PAS domain containing serine/threonine kinase [Sou... [more]
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BLAST of pim-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9YHZ5|PIM2_DANRE2.612e-5438.01Serine/threonine-protein kinase pim-2 OS=Danio rer... [more]
sp|P11309|PIM1_HUMAN2.219e-5238.46Serine/threonine-protein kinase pim-1 OS=Homo sapi... [more]
sp|Q95LJ0|PIM1_FELCA4.395e-5238.10Serine/threonine-protein kinase pim-1 OS=Felis cat... [more]
sp|P26794|PIM1_RAT5.213e-5238.10Serine/threonine-protein kinase pim-1 OS=Rattus no... [more]
sp|Q9N0P9|PIM1_BOVIN8.076e-5238.10Serine/threonine-protein kinase pim-1 OS=Bos tauru... [more]
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BLAST of pim-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2P1ERP60.000e+0100.00PIM-1 OS=Schmidtea mediterranea OX=79327 GN=pim-1 ... [more]
I1ZIJ64.893e-14599.53Pim-2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
T1DBL37.717e-6945.70Pim-1 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
I1ZIJ12.547e-6445.70Pim-3 (Fragment) OS=Schmidtea mediterranea OX=7932... [more]
A0A0R3SR053.504e-6042.36Protein kinase domain-containing protein OS=Hymeno... [more]
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BLAST of pim-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pim13.736e-5742.02serine/threonine-protein kinase pim-1-like [Source... [more]
PIM31.437e-5437.87Pim-3 proto-oncogene, serine/threonine kinase [Sou... [more]
PIM32.151e-5437.37Pim-3 proto-oncogene, serine/threonine kinase [Sou... [more]
pim32.465e-5337.64serine/threonine-protein kinase pim-3-like [Source... [more]
ENSAMXT00000041332.11.378e-4837.45serine/threonine-protein kinase pim-2-like [Source... [more]
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BLAST of pim-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
snrka4.931e-2226.89SNF related kinase a [Source:ZFIN;Acc:ZDB-GENE-040... [more]
ENSPMAT00000006410.13.223e-2128.93pep scaffold:Pmarinus_7.0:GL476370:17405:31068:-1 ... [more]
MARK33.904e-2029.57microtubule affinity regulating kinase 3 [Source:H... [more]
MARK39.804e-2029.18microtubule affinity regulating kinase 3 [Source:H... [more]
phkg1b1.947e-1927.60phosphorylase kinase, gamma 1b (muscle) [Source:ZF... [more]
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BLAST of pim-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
PSK29.944e-2436.88PAS-domain containing serine/threonine protein kin... [more]
PSK14.198e-2331.35PAS domain-containing serine/threonine protein kin... [more]
PKH17.020e-2329.43Serine/threonine protein kinase; involved in sphin... [more]
HSL11.000e-2031.79Nim1p-related protein kinase; septin-binding kinas... [more]
SNF19.788e-2031.13AMP-activated S/T protein kinase; forms a complex ... [more]
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BLAST of pim-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO468612.316e-4437.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO305484.134e-2536.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO307684.881e-2227.59Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO365871.637e-2126.02Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO371433.935e-2129.77Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of pim-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pim19.742e-5741.31serine/threonine-protein kinase pim-1 [Source:NCBI... [more]
pim32.062e-5236.69Pim-3 proto-oncogene, serine/threonine kinase [Sou... [more]
ENSORLT00000032540.12.574e-4837.32serine/threonine-protein kinase pim-2-like [Source... [more]
ENSORLT00000010626.25.928e-4736.11pep primary_assembly:ASM223467v1:12:12000679:12006... [more]
ENSORLT00000041570.14.281e-4538.74pep primary_assembly:ASM223467v1:10:18938984:18940... [more]
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BLAST of pim-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000027798.10.000e+099.65SMESG000027798.1[more]
SMESG000014593.16.965e-6845.70SMESG000014593.1[more]
SMESG000017137.11.183e-6742.32SMESG000017137.1[more]
SMESG000017137.11.212e-6742.32SMESG000017137.1[more]
SMESG000072329.12.888e-5339.64SMESG000072329.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30010205
GenBankAFJ24850.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010205 ID=SMED30010205|Name=pim-2|organism=Schmidtea mediterranea sexual|type=transcript|length=1037bp
TTTGGAACGTATCTTACTGTAAAACATCCTGTTTGCACATTTCAACCAAT
CAACAACATAATTAAATATAAGTTTTAATTTAATTCTTAGTATTTATTAA
ACAATTTCACAATAAATAATTCATATTTTATAAATTTGTTATTGAAATCA
ATAGTAATTAGGAAATGAATCATTCAAAATTTTCAAAAAAACTAGTTGTC
AAGTCAACGGTAAAATATATTCAAAACAAATATGCAATTTATGGGAAAAT
CGGTGAAGGAGGTTTTGGAAAAGTATTTAAATCAACAAAAATCTCAAATG
GAAAATCTGTCGCTTTGAAATTTGTTGCAAAAGACAAAATTTCCGAATAT
TGTTTGCATGAAGAAACTCAACAGAAAATCCCAATGGAAGCGGTTTTATT
AGCGGAATGTCAAGGAATTCCTGGAGTTATTAAACTGTTGGAAATTGTTG
AATTGGAAGAGTTTCAGGTTTGGTGTTTTGTGATGGAAAGAAATCCGAAT
TTCATTGACTTGTTTGACTACATTAATCGATGCAGTCAGATTTCGGAAGA
AACTGCTGTTTTCATTTTAACTCAACTCATTCACACTCTGATTGGATGTC
ACAAAGCCGGAGTCGCTCACAGAGATATCAAGGATGAAAATATTCTGATT
GATGTGGATACGAAAGAAATCAAATTAATTGATTTTGGATCTGGTCATTA
TTATCACGATGGTGAATATTTTGATTTTAATGGAACTTCAATATACAGTC
CTCCTGAATGGATCAAAGACAAACATTATTTCGTTAAAGAATTAGAAAGC
TGGTCAATTGGAGCTTTACTGTATAACATGATAACAGGCGATGGACCTTT
CAAAAATGAAAACGAAATTCTTTTAGCTCCTATATCGATAGATTCTGCTT
CAGCAGAATGTCGAGATTTGATTCTTTCCTGTCTTTGTAGAGATAGAGAA
AACCGAATGGATTTAGACAAAATTTTATGTCATAAATTATTTCAGAAATA
TCACATGTCTATTGGAATTGATGCATGACTATTATTG
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protein sequence of SMED30010205-orf-1

>SMED30010205-orf-1 ID=SMED30010205-orf-1|Name=SMED30010205-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=288bp
MNHSKFSKKLVVKSTVKYIQNKYAIYGKIGEGGFGKVFKSTKISNGKSVA
LKFVAKDKISEYCLHEETQQKIPMEAVLLAECQGIPGVIKLLEIVELEEF
QVWCFVMERNPNFIDLFDYINRCSQISEETAVFILTQLIHTLIGCHKAGV
AHRDIKDENILIDVDTKEIKLIDFGSGHYYHDGEYFDFNGTSIYSPPEWI
KDKHYFVKELESWSIGALLYNMITGDGPFKNENEILLAPISIDSASAECR
DLILSCLCRDRENRMDLDKILCHKLFQKYHMSIGIDA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
GO:0004672protein kinase activity
GO:0005524ATP binding
GO:0004674protein serine/threonine kinase activity
GO:0016301kinase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000719Prot_kinase_dom
IPR017348PIM1/2/3
IPR017441Protein_kinase_ATP_BS
IPR008271Ser/Thr_kinase_AS
IPR011009Kinase-like_dom_sf
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0043066negative regulation of apoptotic process
GO:0016310phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 23..276
e-value: 1.7E-46
score: 170.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 24..276
e-value: 4.7E-51
score: 173.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 23..276
score: 37.714
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 6..111
e-value: 1.3E-20
score: 75.6
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 113..285
e-value: 7.3E-42
score: 144.8
NoneNo IPR availablePANTHERPTHR22984:SF22SERINE/THREONINE-PROTEIN KINASE PRK-2coord: 15..281
NoneNo IPR availablePANTHERPTHR22984SERINE/THREONINE PROTEIN KINASEcoord: 15..281
IPR017348Serine/threonine-protein kinase pim-1/2/3PIRSFPIRSF037993STPK_Pim-1coord: 3..283
e-value: 8.7E-62
score: 206.6
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 29..56
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 150..162
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 19..281