C2H2-type domain-containing protein

Overview
NameC2H2-type domain-containing protein
Smed IDSMED30010203
Length (bp)1237
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of C2H2-type domain-containing protein (SMED30010203) t-SNE clustered cells

Violin plots show distribution of expression levels for C2H2-type domain-containing protein (SMED30010203) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of C2H2-type domain-containing protein (SMED30010203) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for C2H2-type domain-containing protein (SMED30010203) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010203

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30010203h1SMcG0007382 dd_Smed_v4_1455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30010203h1SMcG0007382 dd_Smed_v4_1455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30010203h1SMcG0007382 dd_Smed_v4_1455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010203h1SMcG0007382 dd_Smed_v4_1455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010203h1SMcG0007382 dd_Smed_v4_1455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010203h1SMcG0007382 dd_Smed_v4_1455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30010203h1SMcG0007382 dd_Smed_v4_1455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A267H3W1 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig013893g1 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 4.045e-13
Identity = 66/270 (24.44%), Postives = 102/270 (37.78%), Query Frame = 2 
Query:  395 FPCDLCSQKLNDYNTWITHLSSHEH-----RIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKH------------------KKVNVKEI-------------IINPRSKSGMEF-------------------------CGLCD-QSLSGISDAVDHYNSENHLKLVKNNENSC------WCNICKITLLDESLLEIHLKGFRH-YRILNRE 997
            F C LC+  L D N    HL + +H     +       SS        +++    C +CD  C S    Q H+ GAKH +K++    ++  E K P VYC++C++ L+P LM  H    KH                  KK  V E+              + P    G                            C +CD    +  S A  H+NS+ H    + ++          C +CK+T+  + LLE H KG++H  R +N +
Sbjct:    8 FFCSLCNVNLLDENMLQIHLGTVQHQEMLNKQQRAAASSSNQSGDQPMSVDGALMCSLCDAKCTSEAAYQMHINGAKHAKKVRDAEFQRKKEAKHPDVYCQLCDLTLQPELMQTHLNGMKHLNALKASGDPTAAALITSKKNKVAEVKAMQSMQQKGSAKTLGPMLPPGSNVNHSAALSQMPKAEIQKLLGKRHHMKCSVCDVPEFTTFSQAEHHFNSKAHRVAYQQHQQQMIKAKGKLCELCKVTVSSDELLEAHRKGYKHTLRAMNGQ 277
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A267G5P7 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026513g1 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 4.530e-13
Identity = 66/270 (24.44%), Postives = 102/270 (37.78%), Query Frame = 2 
Query:  395 FPCDLCSQKLNDYNTWITHLSSHEH-----RIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKH------------------KKVNVKEI-------------IINPRSKSGMEF-------------------------CGLCD-QSLSGISDAVDHYNSENHLKLVKNNENSC------WCNICKITLLDESLLEIHLKGFRH-YRILNRE 997
            F C LC+  L D N    HL + +H     +       SS        +++    C +CD  C S    Q H+ GAKH +K++    ++  E K P VYC++C++ L+P LM  H    KH                  KK  V E+              + P    G                            C +CD    +  S A  H+NS+ H    + ++          C +CK+T+  + LLE H KG++H  R +N +
Sbjct:    8 FFCSLCNVNLLDENMLQIHLGTVQHQEMLNKQQRAAASSSNQSGDQPMSVDGALMCSLCDAKCTSEAAYQMHINGAKHAKKVRDAEFQRKKEAKHPDVYCQLCDLTLQPELMQTHLNGMKHLNALKASGDPTAAALITSKKNKVAEVKAMQSMQQKGSAKTLGPMLPPGSNVNHSAALSQMPKAEIQKLLGKRHHMKCSVCDVPEFTTFSQAEHHFNSKAHRVAYQQHQQQMIKAKGKLCELCKVTVSSDELLEAHRKGYKHTLRAMNGQ 277
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A1B6KD30 (Uncharacterized protein OS=Graphocephala atropunctata OX=36148 GN=g.23702 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 6.732e-12
Identity = 61/214 (28.50%), Postives = 100/214 (46.73%), Query Frame = 2 
Query:  290 NCEICKISCNSKSIYDIHTEGKKHKRALEKLSE-----LKFPCDLCSQKLNDYNTWITHLSS--HEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDP--KVYCEICEICLEP-NLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENS 901
            +C+IC +  NS+    IH +GKKHK  +EK          F C++C  K+   +    HL+   H+ ++ + N         P+  +    +C++CD+   S   LQ HL G KHK K ++ N+   +E + P     C+ICE+ L   +L  LH  S +H K  +K  ++    +S  + C +C+ S + I     H     H K  K   N+
Sbjct:  275 DCKICDVKVNSQDQLQIHLDGKKHKDKVEKAKPKAEQFQGFECEICEIKVVSQDQLQIHLAGKKHKEKVEKAN---------PKEEV--WFQCELCDVKAVSQEQLQIHLAGKKHKDKAEKRNVNSSSEAEKPDRPFKCDICEVSLSTKDLFELHTYSKRHVKALLKREML----ESNTDVCDVCNLSFTSIEHYQAHIQGRKHQKKCKETNNT 473

HSP 2 Score: 63.5438 bits (153), Expect = 3.783e-7
Identity = 58/214 (27.10%), Postives = 91/214 (42.52%), Query Frame = 2 
Query:  401 CDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEI-CLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHL-KLVKNNENSCW----------CNICKITLLDESLLEIHLKGFRHYRILNREECI 1006
            CD C  +    +  + HL+S +H+             +P+    ++  C ICD+   S   LQ HL G KHK K+++    K    +     CEICEI  +  + + +H    KHK+   K    NP+ +   + C LCD           H   + H  K  K N NS            C+IC+++L  + L E+H    RH + L + E +
Sbjct:  235 CDFCDVRFVSQDQLLVHLASKKHQ-------KKVEKPIPKVEEFQVFDCKICDVKVNSQDQLQIHLDGKKHKDKVEKA---KPKAEQFQGFECEICEIKVVSQDQLQIHLAGKKHKEKVEK---ANPKEEVWFQ-CELCDVKAVSQEQLQIHLAGKKHKDKAEKRNVNSSSEAEKPDRPFKCDICEVSLSTKDLFELHTYSKRHVKALLKREML 434
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A1I8F384 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.823e-10
Identity = 60/237 (25.32%), Postives = 95/237 (40.08%), Query Frame = 2 
Query:  545 CDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEI-------IINPRSKSGMEFC----------------------------------------GLCDQSLSGISDAVD-HYNSENH----LKLVKNN--ENSCWCNICKITLLDESLLEIHLKGFRHYRILNREECISKTGTY---KEKMLYKRMKQKELRVQYEK 1084
            C++CD  C    + + H  G+KH +K K+    +L+E KDP+VYC+IC + L P LM  H+   KH+K     +         +P  KS +                                           G   Q++S  S   + H+NS+ H     KL ++        C +C I +  + L+E HLKG+RH         + + G Y     +   K MK++E  + Y K
Sbjct:   94 CELCDAKCTGEAMFKLHREGSKHLKKEKEKKFRELSEAKDPEVYCDICNLTLHPELMKSHRAGVKHRKAAAGALNRGGSGAKKSPTGKSALPPGIYRLDPAKSAADKDKPPGGATEASSSSAANETAATADAQNGTQQQAISTRSKRAELHFNSKAHRMAFQKLQRDQIRSKGFVCEVCDIAVTSQDLMEAHLKGYRH--------ALRQHGGYIPEGHEFYAKYMKRQEAMLSYRK 322
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: M5W496 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa008691mg PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 9.395e-10
Identity = 52/164 (31.71%), Postives = 75/164 (45.73%), Query Frame = 2 
Query:  545 CDICDLDCGSNIILQSHLTGAKHK---RKLKQLNIEKLAENKDPKVYCEICEI-CLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKN-----------NENSCWCNICKITLLDESLLEIHLKGFRHYRILN 991
            C+IC + C   I L SHL G KHK   ++L+Q N  +     DP ++C IC + C E N M  H    KHK+ NV+E   NPR      +C +C    SG      H N + H   V+            N+   WC +C ++   E  +  HL   +H+  L+
Sbjct:  154 CNICLVGCSGKIDLMSHLNGRKHKENVQELQQQNANEAPRKNDPPLWCNICHVRCSESN-MASHLNGRKHKE-NVQE-PQNPRKNEPPLWCKICSVGCSGNIHLASHLNGKKHKDKVQEQQQNADIVQWKNDPPLWCKVCDVSCYTEFDMASHLNRRKHWERLS 314
[Close]
BLAST of C2H2-type domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000010660.2 (zinc finger matrin-type protein 1 [Source:NCBI gene;Acc:101173296])

HSP 1 Score: 54.299 bits (129), Expect = 1.501e-7
Identity = 57/255 (22.35%), Postives = 99/255 (38.82%), Query Frame = 2 
Query:  338 IHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEH----RIAELNILSSTTCNLPRTTLNKINR-CDICDLDCGSNIILQSHLTGAKHKRKLKQLNI----EKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGM---------EFCGLCDQSLSGISDAVDHYNSENHLKLVKNNE------------NSCWCNICKITLLDESLLEIHLKGFRHYRILNREECISK 1012
            +H EG+K    L +       C +CS KL   +  ++H    +H    ++    +       + RT     NR C++C++   S+++ +SH  G  H + L++  +    +K  E   P    +I +         LH               ++P + +G           +C LC  S +    A+ HYN   H + V   E            NS  C IC +      + + H++G RH   L RE+ IS+
Sbjct:   41 VHVEGEKSDEQLLQGLLTDTYCHVCSSKLLFESHRLSHYEGKKHAQKVKVYLKTLRGEKNAEVKRTLQTNKNRFCELCNMVFSSDVVAKSHYEGKTHAKTLRKHTLHPPDQKSEELPSPNPAGKIEQKAASDVGTELH---------------LDPTAATGSTNSSLKDPDRYCSLCAASFNNPQMALQHYNGRKHQRNVARQELLKELGGDGQQANSLMCQICGVPFDSVEMYQAHMQGNRH---LTREKKISE 277
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000067728.1 (SMESG000067728.1)

HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0
Identity = 310/312 (99.36%), Postives = 311/312 (99.68%), Query Frame = 2 
Query:  167 MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITLLDESLLEIHLKGFRHYRILNREECISKTGTYKEKMLYKRMKQKELRVQYEKEKLEQF 1102
            MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLN+EKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITL DESLLEIHLKGFRHYRILNREECISKTGTYKEKMLYKRMKQKELRVQYEKEKLEQF
Sbjct:    1 MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNMEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITLPDESLLEIHLKGFRHYRILNREECISKTGTYKEKMLYKRMKQKELRVQYEKEKLEQF 312
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000067728.1 (SMESG000067728.1)

HSP 1 Score: 538.495 bits (1386), Expect = 0.000e+0
Identity = 265/271 (97.79%), Postives = 268/271 (98.89%), Query Frame = 2 
Query:  167 MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITLLDESLLEIHLKGFRHY 979
            MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLN+EKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITL DESLLEIHLK  R++
Sbjct:    1 MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNMEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITLPDESLLEIHLKEKRYF 271
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000067728.1 (SMESG000067728.1)

HSP 1 Score: 538.11 bits (1385), Expect = 0.000e+0
Identity = 265/274 (96.72%), Postives = 269/274 (98.18%), Query Frame = 2 
Query:  167 MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITLLDESLLEIHLKGFRHYRIL 988
            MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLN+EKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITL DESLLEIHLK F +  ++
Sbjct:    1 MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNMEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITLPDESLLEIHLKEFIYETVI 274
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000067728.1 (SMESG000067728.1)

HSP 1 Score: 534.258 bits (1375), Expect = 0.000e+0
Identity = 264/268 (98.51%), Postives = 265/268 (98.88%), Query Frame = 2 
Query:  167 MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITLLDESLLEIHLKGF 970
            MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLN+EKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITL DESLLEIHLK  
Sbjct:    1 MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNMEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNICKITLPDESLLEIHLKDL 268
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000067725.1 (SMESG000067725.1)

HSP 1 Score: 145.976 bits (367), Expect = 6.546e-40
Identity = 87/281 (30.96%), Postives = 147/281 (52.31%), Query Frame = 2 
Query:  284 PHNCEICKISCNSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKL--NDYNTWITHLSSHEHRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHKRKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEIIINPRSKSG--------MEFCGLCDQSLSGISDAVDHYNSENH-LKLVKNNEN-SCWCNICKITLLDESLLEIHLKGFRHYRILNREECISKTGTYKEKMLYKRMKQKELRVQYEKEK 1090
            P  C++C I+C+S++ YD H +GKKH +AL +    +      + K+  ND    + +       +++ N++                 C+ICD++C     L+ HL G++H++KLK     +L  NKDP V+C+ICE  +    M +H+ S KHK+ +  E+   P  K          +E CG C  SL   SDA+ HY ++ H L +   + N   WC++C+I+  ++S L++HL GF+H+  +NR     + G + E+++  R K + L+ Q  KEK
Sbjct:   63 PVQCDVCNITCSSQTTYDFHIKGKKHTKALSRKDLFENGKTASNAKVEGNDCMDPVEYSDQQGQILSKSNLM-----------------CEICDVNCLRPEQLERHLEGSRHRKKLKLNRYIELRNNKDPSVFCDICESFMNEESMNMHRASGKHKRKSSAEVKDEPAEKKARSADIPTTVEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRNFELWCDVCQISFTNQSFLDLHLTGFKHFVRVNRGNSAPE-GAFGERLMKMRAKLQSLK-QSRKEK 324
The following BLAST results are available for this feature:
BLAST of C2H2-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267H3W14.045e-1324.44Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267G5P74.530e-1324.44Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1B6KD306.732e-1228.50Uncharacterized protein OS=Graphocephala atropunct... [more]
A0A1I8F3843.823e-1025.32Uncharacterized protein OS=Macrostomum lignano OX=... [more]
M5W4969.395e-1031.71Uncharacterized protein OS=Prunus persica OX=3760 ... [more]
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BLAST of C2H2-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000010660.21.501e-722.35zinc finger matrin-type protein 1 [Source:NCBI gen... [more]
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BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067728.10.000e+099.36SMESG000067728.1[more]
SMESG000067728.10.000e+097.79SMESG000067728.1[more]
SMESG000067728.10.000e+096.72SMESG000067728.1[more]
SMESG000067728.10.000e+098.51SMESG000067728.1[more]
SMESG000067725.16.546e-4030.96SMESG000067725.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010203 ID=SMED30010203|Name=C2H2-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1237bp
ATGCAAACAGTGCAAATTCAAAAACCAAGATTTAATATTATGTAATATTA
AATTTATGTTGAAGTAAATATTTGATAATTAATTTTAAATAATGTATTGC
TTAATGTCATATAAATTTTTCTTTTATAACCATTGTTATATTTAATTCTA
TTCTAATTATGAAGATATGAATTCTCGAAGAGTTAAATTTACAGAAACAA
ATATTGTGCATGATATTCCTATTGACAAAAATAGTGTTAATGCAAGATCA
AAATTTACTCGTATTAGGCCTACAAAATATGATCCCCATAATTGTGAAAT
ATGCAAAATATCGTGCAATTCCAAAAGCATATATGATATTCATACTGAAG
GTAAAAAGCATAAAAGAGCTTTAGAAAAGTTATCCGAGTTGAAGTTTCCA
TGTGATTTGTGCTCTCAGAAACTTAATGATTATAATACATGGATCACTCA
TCTATCGTCTCATGAGCACAGAATTGCTGAACTTAACATTCTTTCTTCAA
CTACCTGTAATTTACCTAGAACAACATTAAATAAAATTAATCGTTGTGAT
ATTTGTGATCTGGACTGTGGAAGTAACATCATTTTACAGTCTCATTTGAC
TGGGGCAAAACACAAACGTAAACTTAAGCAATTAAACATTGAAAAACTAG
CGGAAAACAAAGACCCGAAAGTTTATTGTGAAATATGTGAAATATGTTTA
GAGCCGAATTTAATGATGTTACACAAACAAAGTAATAAACACAAAAAAGT
CAACGTGAAAGAAATTATAATTAATCCGAGATCGAAAAGTGGCATGGAAT
TCTGCGGTTTATGTGACCAATCATTATCGGGAATCTCAGACGCAGTAGAC
CACTACAATTCTGAAAATCATTTGAAACTTGTAAAAAATAATGAAAATTC
CTGTTGGTGTAATATCTGCAAGATTACTTTGCTTGATGAATCGTTGCTGG
AAATACATTTGAAAGGGTTTAGGCACTATCGGATTCTGAATAGGGAGGAG
TGTATTAGTAAAACTGGCACCTATAAAGAGAAGATGCTATATAAACGTAT
GAAACAGAAAGAGTTGCGGGTTCAATATGAAAAAGAAAAATTAGAACAAT
TTTAAGAAATAAGTTGCTTAATGAAAGGCTGTTTATAATAATTGTAAATA
GGGGGCTTTTTGAATTATTTTTATCAATTCAAGTTTTATTACTATTTGTT
ATTTAATTTTTTTAATAAATATTCATTTTGATTGAAA
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protein sequence of SMED30010203-orf-1

>SMED30010203-orf-1 ID=SMED30010203-orf-1|Name=SMED30010203-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=313bp
MNSRRVKFTETNIVHDIPIDKNSVNARSKFTRIRPTKYDPHNCEICKISC
NSKSIYDIHTEGKKHKRALEKLSELKFPCDLCSQKLNDYNTWITHLSSHE
HRIAELNILSSTTCNLPRTTLNKINRCDICDLDCGSNIILQSHLTGAKHK
RKLKQLNIEKLAENKDPKVYCEICEICLEPNLMMLHKQSNKHKKVNVKEI
IINPRSKSGMEFCGLCDQSLSGISDAVDHYNSENHLKLVKNNENSCWCNI
CKITLLDESLLEIHLKGFRHYRILNREECISKTGTYKEKMLYKRMKQKEL
RVQYEKEKLEQF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0046872metal ion binding
GO:0003676nucleic acid binding
GO:0008270zinc ion binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002056medial muscle cell
PLANA:0003001lateral region of the whole animal
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013087Znf_C2H2_type
IPR036236Znf_C2H2_sf
IPR003604Matrin/U1-like-C_Znf_C2H2
IPR015880Znf_C2H2-like
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 125..149
e-value: 13.0
score: 12.1
coord: 211..235
e-value: 150.0
score: 2.9
coord: 77..99
e-value: 2.9
score: 16.9
coord: 246..270
e-value: 38.0
score: 8.2
coord: 41..65
e-value: 11.0
score: 12.8
IPR003604Matrin/U1-C-like, C2H2-type zinc fingerSMARTSM00451ZnF_U1_5coord: 166..200
e-value: 1.3
score: 11.3
coord: 38..72
e-value: 5.8E-4
score: 29.2
coord: 243..277
e-value: 5.8
score: 6.2
coord: 208..242
e-value: 4.6
score: 6.9
coord: 74..108
e-value: 27.0
score: 0.8
coord: 122..156
e-value: 0.086
score: 20.7
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 27..76
e-value: 1.5E-10
score: 42.4
coord: 239..288
e-value: 9.3E-6
score: 27.1
coord: 115..162
e-value: 4.6E-9
score: 37.6
NoneNo IPR availablePFAMPF12874zf-metcoord: 43..65
e-value: 2.2E-6
score: 27.9
coord: 247..267
e-value: 4.6E-4
score: 20.5
coord: 127..149
e-value: 0.0041
score: 17.5
NoneNo IPR availablePANTHERPTHR46144FAMILY NOT NAMEDcoord: 39..73
coord: 121..159
coord: 159..273
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 120..160
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 34..72
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 243..274