NPHS1-3
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010202 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of NPHS1-3 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 97.0561 bits (240), Expect = 1.471e-19 Identity = 127/578 (21.97%), Postives = 215/578 (37.20%), Query Frame = 3
Query: 1587 GDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLI----INVIFPPVHLEISCLNCIPNKIMANRTNEFL---CESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLY-PPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS----------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVK----TSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSK--QGTDISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
G + L C + GGNP P+L WYK++ V S + + L + + + + V S+N K+ L + V FPP ++ I A R + L C S SSNP ++W + ++G + + G + L ++ + + + C++ + E + + +L VLY P F+ + + E L +SANP F+W F+ +S S L + N+ D G Y+ +N G+A +DV Y+P + T +N+ S + C NPI W R+ ++ D + + + PT ++RI+ ++KL + G YQC+ NG+ R L L + P + +K D + + L C N W VP + T T + L + V + + C A N GS + + L+ +PD P K+ L +++ L W G DGG Q F +R+
Sbjct: 458 GTRVRLVCLAIGGNPEPSLMWYKDSRTVTES-RLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILA------NASALRPGDALNLTCVSVSSNPPVNLSW--------------DKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSA-----ELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNV-GGSVDIVCTVDANPILPGM-FNWERLGEDEEDQSLDDMEKI-----------SRGPTGRLRIH-----------HAKL-------AQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQ-TNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY 977 HSP 2 Score: 59.3066 bits (142), Expect = 6.106e-8 Identity = 28/75 (37.33%), Postives = 39/75 (52.00%), Query Frame = 3
Query: 201 SIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLL 425
S+ + C V+ VQW L+LG D +PG PR ++ GD +GE +L I D +YECQV + P L
Sbjct: 48 SVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPEL 122
BLAST of NPHS1-3 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 97.0561 bits (240), Expect = 1.743e-19 Identity = 127/578 (21.97%), Postives = 215/578 (37.20%), Query Frame = 3
Query: 1587 GDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLI----INVIFPPVHLEISCLNCIPNKIMANRTNEFL---CESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLY-PPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS----------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVK----TSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSK--QGTDISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
G + L C + GGNP P+L WYK++ V S + + L + + + + V S+N K+ L + V FPP ++ I A R + L C S SSNP ++W + ++G + + G + L ++ + + + C++ + E + + +L VLY P F+ + + E L +SANP F+W F+ +S S L + N+ D G Y+ +N G+A +DV Y+P + T +N+ S + C NPI W R+ ++ D + + + PT ++RI+ ++KL + G YQC+ NG+ R L L + P + +K D + + L C N W VP + T T + L + V + + C A N GS + + L+ +PD P K+ L +++ L W G DGG Q F +R+
Sbjct: 458 GTRVRLVCLAIGGNPEPSLMWYKDSRTVTES-RLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILA------NASALRPGDALNLTCVSVSSNPPVNLSW--------------DKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSA-----ELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNV-GGSVDIVCTVDANPILPGM-FNWERLGEDEEDQSLDDMEKI-----------SRGPTGRLRIH-----------HAKL-------AQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQ-TNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY 977 HSP 2 Score: 59.3066 bits (142), Expect = 6.000e-8 Identity = 28/75 (37.33%), Postives = 39/75 (52.00%), Query Frame = 3
Query: 201 SIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLL 425
S+ + C V+ VQW L+LG D +PG PR ++ GD +GE +L I D +YECQV + P L
Sbjct: 48 SVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPEL 122
BLAST of NPHS1-3 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 68.1662 bits (165), Expect = 3.772e-12 Identity = 35/101 (34.65%), Postives = 46/101 (45.54%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYIL 479
D VL+ + + C + G VQW L LG R +PG R I G+ G+H+L I + ED YECQ L R + LHV P P +L
Sbjct: 32 DLVVLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQATQAG---LRSRPAQLHVLVPPEAPQVL 129
BLAST of NPHS1-3 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 68.5514 bits (166), Expect = 6.272e-11 Identity = 43/143 (30.07%), Postives = 56/143 (39.16%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEG-GLPQPIFQWF 602
D VL+ + + C + G VQW L LG R +PG R I G+ G+H+L I + ED YECQ L R + LHV P P +L ++ L C S G P P WF
Sbjct: 32 DLVVLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQATQAG---LRSRPAQLHVLVPPEAPQVL----------GGPSVSLVAGVPANLTCRSRGDARPTPELLWF 161
BLAST of NPHS1-3 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 68.1662 bits (165), Expect = 7.865e-11 Identity = 43/143 (30.07%), Postives = 56/143 (39.16%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEG-GLPQPIFQWF 602
D VL+ + + C + G VQW L LG R +PG R I G+ G+H+L I + ED YECQ L R + LHV P P +L ++ L C S G P P WF
Sbjct: 32 DLVVLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQATQAG---LRSRPAQLHVLVPPEAPQVL----------GGPSVSLVAGVPANLTCRSRGDARPTPELLWF 161
BLAST of NPHS1-3 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 130.183 bits (326), Expect = 3.411e-30 Identity = 136/611 (22.26%), Postives = 258/611 (42.23%), Query Frame = 3
Query: 1464 IECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFS-DEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSW--------YFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTS---INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII------FSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
+EC AT KQ + K +I+G + EGDI+++TC + GGNP L WY+ + V + +E+ TL RV + N Q++ CEA + + ++S +L +V +PP + I + +++ ++ +C + SSNP + I+W+ + + V G +S N + NG+ ++ L+ + + ++ C + +H PE + + L V+YPP ++N ++ + EG + + NP + + W + R + L K + D+G Y N +GS + ++ V+YS ++ S I D+ CEA P +N + W Y++ IL+ + + + +R+N+ E G Y C NGIG+ L + P L +++Y+K + + C + P++ ++ ++I +S T+I ST+ L + + + Y + KC A+N G+ L+ + ++P PD P + + N I ++W DGGS+Q F++++
Sbjct: 106 VECMATNPEGSSAKQHV-IKIIAPPKAPIITG-LEDRKFFEGDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYSTLAL--RVDRTMNTQRLKCEATNAALDEPLIESQYL--SVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATD---------DNKVHLGDVSLNETTRDNGFNVENVLSFTPTEEYDGTVVHCIA--NH---PEWKHSVNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANM-IHW----------YKNGE----ILAAQHRGHKKAI----LRLNAT-------------------EQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKV--YAVPTVEFVWEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLS-IPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 655
BLAST of NPHS1-3 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 130.183 bits (326), Expect = 4.631e-30 Identity = 136/611 (22.26%), Postives = 258/611 (42.23%), Query Frame = 3
Query: 1464 IECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFS-DEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSW--------YFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTS---INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII------FSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
+EC AT KQ + K +I+G + EGDI+++TC + GGNP L WY+ + V + +E+ TL RV + N Q++ CEA + + ++S +L +V +PP + I + +++ ++ +C + SSNP + I+W+ + + V G +S N + NG+ ++ L+ + + ++ C + +H PE + + L V+YPP ++N ++ + EG + + NP + + W + R + L K + D+G Y N +GS + ++ V+YS ++ S I D+ CEA P +N + W Y++ IL+ + + + +R+N+ E G Y C NGIG+ L + P L +++Y+K + + C + P++ ++ ++I +S T+I ST+ L + + + Y + KC A+N G+ L+ + ++P PD P + + N I ++W DGGS+Q F++++
Sbjct: 408 VECMATNPEGSSAKQHV-IKIIAPPKAPIITG-LEDRKFFEGDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYSTLAL--RVDRTMNTQRLKCEATNAALDEPLIESQYL--SVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATD---------DNKVHLGDVSLNETTRDNGFNVENVLSFTPTEEYDGTVVHCIA--NH---PEWKHSVNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANM-IHW----------YKNGE----ILAAQHRGHKKAI----LRLNAT-------------------EQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKV--YAVPTVEFVWEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLS-IPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 957 HSP 2 Score: 60.8474 bits (146), Expect = 8.170e-9 Identity = 57/252 (22.62%), Postives = 100/252 (39.68%), Query Frame = 3
Query: 1227 IISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHA-TTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSI 1979
II+ EN +N+ C + + W ++DG +++S +K N T + + ++ R + I C A E I + T +V + + + + GD + + C GGNP P + WY N + + T +E + +Y N E ++ N + K + + V +PP +E+ + I AN C+S SNP S ITW ++N S+
Sbjct: 139 IIAVKENTPLNITCVVPNVKPEPEVLW------YMDG-----KVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKE--IKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFGESNIRYGSSAN----IQCKSLPSNPASQITW-IINGRSV 372
BLAST of NPHS1-3 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 124.79 bits (312), Expect = 1.292e-28 Identity = 135/622 (21.70%), Postives = 255/622 (41.00%), Query Frame = 3
Query: 1464 IECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIK------TDKEEQTLDF------LYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSW--------YFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTS---INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII------FSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
+EC AT KQ + K +I+G + EGDI+++TC + GGNP L WY+ + L K + F RV + N Q++ CEA + + ++S +L +V +PP + I + +++ ++ +C + SSNP + I+W+ + + V G +S N + NG+ ++ L+ + + ++ C + +H PE + + L V+YPP ++N ++ + EG + + NP + + W + R + L K + D+G Y N +GS + ++ V+YS ++ S I D+ CEA P +N + W Y++ IL+ + + + +R+N+ E G Y C NGIG+ L + P L +++Y+K + + C + P++ ++ ++I +S T+I ST+ L + + + Y + KC A+N G+ L+ + ++P PD P + + N I ++W DGGS+Q F++++
Sbjct: 106 VECMATNPEGSSAKQHV-IKIIAPPKAPIITG-LEDRKFFEGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQYL--SVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATD---------DNKVHLGDVSLNETTRDNGFNVENVLSFTPTEEYDGTVVHCIA--NH---PEWKHSVNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANM-IHW----------YKNGE----ILAAQHRGHKKAI----LRLNAT-------------------EQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKV--YAVPTVEFVWEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLS-IPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 668
BLAST of NPHS1-3 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 125.176 bits (313), Expect = 1.479e-28 Identity = 135/622 (21.70%), Postives = 255/622 (41.00%), Query Frame = 3
Query: 1464 IECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIK------TDKEEQTLDF------LYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSW--------YFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTS---INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII------FSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
+EC AT KQ + K +I+G + EGDI+++TC + GGNP L WY+ + L K + F RV + N Q++ CEA + + ++S +L +V +PP + I + +++ ++ +C + SSNP + I+W+ + + V G +S N + NG+ ++ L+ + + ++ C + +H PE + + L V+YPP ++N ++ + EG + + NP + + W + R + L K + D+G Y N +GS + ++ V+YS ++ S I D+ CEA P +N + W Y++ IL+ + + + +R+N+ E G Y C NGIG+ L + P L +++Y+K + + C + P++ ++ ++I +S T+I ST+ L + + + Y + KC A+N G+ L+ + ++P PD P + + N I ++W DGGS+Q F++++
Sbjct: 408 VECMATNPEGSSAKQHV-IKIIAPPKAPIITG-LEDRKFFEGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQYL--SVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATD---------DNKVHLGDVSLNETTRDNGFNVENVLSFTPTEEYDGTVVHCIA--NH---PEWKHSVNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANM-IHW----------YKNGE----ILAAQHRGHKKAI----LRLNAT-------------------EQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKV--YAVPTVEFVWEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLS-IPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 970 HSP 2 Score: 60.8474 bits (146), Expect = 8.192e-9 Identity = 57/252 (22.62%), Postives = 100/252 (39.68%), Query Frame = 3
Query: 1227 IISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHA-TTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSI 1979
II+ EN +N+ C + + W ++DG +++S +K N T + + ++ R + I C A E I + T +V + + + + GD + + C GGNP P + WY N + + T +E + +Y N E ++ N + K + + V +PP +E+ + I AN C+S SNP S ITW ++N S+
Sbjct: 139 IIAVKENTPLNITCVVPNVKPEPEVLW------YMDG-----KVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKE--IKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFGESNIRYGSSAN----IQCKSLPSNPASQITW-IINGRSV 372
BLAST of NPHS1-3 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 101.293 bits (251), Expect = 2.169e-21 Identity = 110/522 (21.07%), Postives = 218/522 (41.76%), Query Frame = 3
Query: 1728 RVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSW--------YFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTS---INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII------FSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
RV + N Q++ CEA + + ++S +L +V +PP + I + +++ ++ +C + SSNP + I+W+ + + V G +S N + NG+ ++ L+ + + ++ C + +H PE + + L V+YPP ++N ++ + EG + + NP + + W + R + L K + D+G Y N +GS + ++ V+YS ++ S I D+ CEA P +N + W Y++ IL+ + + + +R+N+ E G Y C NGIG+ L + P L +++Y+K + + C + P++ ++ ++I +S T+I ST+ L + + + Y + KC A+N G+ L+ + ++P PD P + + N I ++W DGGS+Q F++++
Sbjct: 25 RVDRTMNTQRLKCEATNAALDEPLIESQYL--SVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATD---------DNKVHLGDVSLNETTRDNGFNVENVLSFTPTEEYDGTVVHCIA--NH---PEWKHSVNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANM-IHW----------YKNGE----ILAAQHRGHKKAI----LRLNAT-------------------EQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKV--YAVPTVEFVWEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLS-IPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 489
BLAST of NPHS1-3 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087413) HSP 1 Score: 194.512 bits (493), Expect = 7.585e-50 Identity = 237/1105 (21.45%), Postives = 412/1105 (37.29%), Query Frame = 3
Query: 147 IQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRF---------------EAKVTVIMFVRSGNPLIQNSNQ--IVDENNDMEFTCSMKPTGHPKSKLKWSMN--------ITAGFRMENLIK--IKYNENQDELI---TNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYV-FEGSTVNLQYQISANPKATHFSWYFQN-----------RFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSIN---IFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWL-----VPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEI 3311
+Q F P D +L + C V+N+ GKVQW LG+ +PG PR ++ D + +NL+I A ED +Y+CQV P +P +R + L + PS I + V ++ L L C++E P WF EV + + P + +T L+ R + Y+ F A+V + + G P + +Q + +++ C + G+P ++L W N T+G EN+ K +N L+ NLL R + V + +++ ++ Q V + Q LS VT NP + S+ IN +S Y T + G +V S +++ I S + +++ H + T +TQ + T+N V Y +++GY + G I+ L C S GGNP PTL+WYKN+ ++ ++ K L L V DN C+ ++ + + + L V FPP ++IS +P ++ + +C+SSSSNP + I+W ++G N PG + G + ++ + + + + CQS +++L + + + I L +LYPP ++ +V EG+ +++ S NP ++W R IS L I + ND+G Y N G+A ++ V+Y+P + S + + A C P+ + V W SRD + + S N + L I+ + SD G + C+ N G +N+ L + P +++ + + L C L+ PS W + F + ++ F L + +GQY+C +N G + P+ +PD P ++ + + L+W G DGG + F+LR KQ +Y+ V + + +
Sbjct: 38 VQKFHLTP-HDLQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVI-------EGYARNARVEVEERQNLTLHCIAENANPAAEIVWFQG--EVPVSTAPIVTVNQTAPKR-------------------FTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSR-GGNPPAQLTWYRNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGAN---QAKVGDSVQ-LSCVT---APSNPQARISW------------------SINGRPLDNSTYKTTSSS----DGGWVSSSNISLTIDS---QSRTFIAVCHALNTELTQNVVGSHTVN-----------------------VLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLL--VNASDNNAIYKCKVQNAAIDIPLFATKTL--GVHFPPETVKIS---VVPKNLVPGIRAKLICDSSSSNPPAKISWWK--------------DGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSH--NEVL---QRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLEAAH-VRW-------------SRDGYDLATRTISSFENGT--------------------ALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPS-RPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRL---KQHGEDKYKYVDAKPGHQNISL 993
BLAST of NPHS1-3 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087414) HSP 1 Score: 194.126 bits (492), Expect = 1.021e-49 Identity = 237/1105 (21.45%), Postives = 412/1105 (37.29%), Query Frame = 3
Query: 147 IQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRF---------------EAKVTVIMFVRSGNPLIQNSNQ--IVDENNDMEFTCSMKPTGHPKSKLKWSMN--------ITAGFRMENLIK--IKYNENQDELI---TNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYV-FEGSTVNLQYQISANPKATHFSWYFQN-----------RFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSIN---IFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWL-----VPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEI 3311
+Q F P D +L + C V+N+ GKVQW LG+ +PG PR ++ D + +NL+I A ED +Y+CQV P +P +R + L + PS I + V ++ L L C++E P WF EV + + P + +T L+ R + Y+ F A+V + + G P + +Q + +++ C + G+P ++L W N T+G EN+ K +N L+ NLL R + V + +++ ++ Q V + Q LS VT NP + S+ IN +S Y T + G +V S +++ I S + +++ H + T +TQ + T+N V Y +++GY + G I+ L C S GGNP PTL+WYKN+ ++ ++ K L L V DN C+ ++ + + + L V FPP ++IS +P ++ + +C+SSSSNP + I+W ++G N PG + G + ++ + + + + CQS +++L + + + I L +LYPP ++ +V EG+ +++ S NP ++W R IS L I + ND+G Y N G+A ++ V+Y+P + S + + A C P+ + V W SRD + + S N + L I+ + SD G + C+ N G +N+ L + P +++ + + L C L+ PS W + F + ++ F L + +GQY+C +N G + P+ +PD P ++ + + L+W G DGG + F+LR KQ +Y+ V + + +
Sbjct: 38 VQKFHLTP-HDLQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVI-------EGYARNARVEVEERQNLTLHCIAENANPAAEIVWFQG--EVPVSTAPIVTVNQTAPKR-------------------FTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSR-GGNPPAQLTWYRNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGAN---QAKVGDSVQ-LSCVT---APSNPQARISW------------------SINGRPLDNSTYKTTSSS----DGGWVSSSNISLTIDS---QSRTFIAVCHALNTELTQNVVGSHTVN-----------------------VLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLL--VNASDNNAIYKCKVQNAAIDIPLFATKTL--GVHFPPETVKIS---VVPKNLVPGIRAKLICDSSSSNPPAKISWWK--------------DGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSH--NEVL---QRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLEAAH-VRW-------------SRDGYDLATRTISSFENGT--------------------ALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPS-RPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRL---KQHGEDKYKYVDAKPGHQNISL 993
BLAST of NPHS1-3 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0345419) HSP 1 Score: 194.126 bits (492), Expect = 1.021e-49 Identity = 237/1105 (21.45%), Postives = 412/1105 (37.29%), Query Frame = 3
Query: 147 IQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRF---------------EAKVTVIMFVRSGNPLIQNSNQ--IVDENNDMEFTCSMKPTGHPKSKLKWSMN--------ITAGFRMENLIK--IKYNENQDELI---TNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYV-FEGSTVNLQYQISANPKATHFSWYFQN-----------RFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSIN---IFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWL-----VPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEI 3311
+Q F P D +L + C V+N+ GKVQW LG+ +PG PR ++ D + +NL+I A ED +Y+CQV P +P +R + L + PS I + V ++ L L C++E P WF EV + + P + +T L+ R + Y+ F A+V + + G P + +Q + +++ C + G+P ++L W N T+G EN+ K +N L+ NLL R + V + +++ ++ Q V + Q LS VT NP + S+ IN +S Y T + G +V S +++ I S + +++ H + T +TQ + T+N V Y +++GY + G I+ L C S GGNP PTL+WYKN+ ++ ++ K L L V DN C+ ++ + + + L V FPP ++IS +P ++ + +C+SSSSNP + I+W ++G N PG + G + ++ + + + + CQS +++L + + + I L +LYPP ++ +V EG+ +++ S NP ++W R IS L I + ND+G Y N G+A ++ V+Y+P + S + + A C P+ + V W SRD + + S N + L I+ + SD G + C+ N G +N+ L + P +++ + + L C L+ PS W + F + ++ F L + +GQY+C +N G + P+ +PD P ++ + + L+W G DGG + F+LR KQ +Y+ V + + +
Sbjct: 38 VQKFHLTP-HDLQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVI-------EGYARNARVEVEERQNLTLHCIAENANPAAEIVWFQG--EVPVSTAPIVTVNQTAPKR-------------------FTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSR-GGNPPAQLTWYRNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGAN---QAKVGDSVQ-LSCVT---APSNPQARISW------------------SINGRPLDNSTYKTTSSS----DGGWVSSSNISLTIDS---QSRTFIAVCHALNTELTQNVVGSHTVN-----------------------VLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLL--VNASDNNAIYKCKVQNAAIDIPLFATKTL--GVHFPPETVKIS---VVPKNLVPGIRAKLICDSSSSNPPAKISWWK--------------DGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSH--NEVL---QRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLEAAH-VRW-------------SRDGYDLATRTISSFENGT--------------------ALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPS-RPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRL---KQHGEDKYKYVDAKPGHQNISL 993
BLAST of NPHS1-3 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0088635) HSP 1 Score: 174.481 bits (441), Expect = 1.718e-43 Identity = 235/1113 (21.11%), Postives = 408/1113 (36.66%), Query Frame = 3
Query: 27 EKKMYQIWI----VIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLP-SIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGH---QILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQN--SNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNP-NEKSSFPPFIQQNHLDIISGIE---NEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKL-KRTLNSGFIECHAT----TENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQS------RLSHKILPEKYKPIVKNIKLKVLY-PPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWY---------FQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWL-----VPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
++ Q WI ++ L + I+ S+P+P Q Q F+ P D VL + C V N G VQW LG+ +PG PR ++GD +G +NL+I+ A D DY+CQV P + +R + L V P SI Y + D L+L C+ P W+ + E ++ E + + ++ S + C ++KA S A V + + G P I+ + + + +E C + G+P ++L W ++ + I++ Y RT R +SE+ + F N + N S NP + H+ ++ E + + + C T+ +KW++ + + TI +SP T + IT ++ KR+L + CH TEN + NV Y +ISGY+ + G + L C S GGNP TL WYKN+ + + I+ + + + DN + CEA + ++ +S L ++ V + I P ++ +C+SSSSNP + ++W ++G +T+ PG + G + + + + + N + CQS R +H+ + L VLY P F+ ++ EG ++ + Q ANP + W +R + +L +H +D+G Y N G AT + + V+Y + S NI + A C P+ V W +R+ Y D S ++ + S L I + D G ++C+ N + ++ L + P K + + L C P W +P + + +I T+ L + + +G Y+C A+NE G + + V L T QPD P S + + + + L+W G DGG + ++++R+
Sbjct: 38 RRRCRQHWIKHLQLLRLLAVIVLASAPVPSHAQQ-----------QKFRTTP-HDLQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHNSKVEVRENQD--------LQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKAL--------SPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSR-GGNPPAQLIW-------YKNGSQIRMAY-----------------RTSGR-----LSENIYT------FTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTI--VSP--------EGGWTTTSNITAVVEPNKRSL---VVICHGLNMQLTENVV---STHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRI--NSVIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIE-----PEELRPGMEATIICDSSSSNPPAKLSWWK--------------DGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEA-----------VSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAMLSCTVEGKPLTEEH-VKW------ERVGY----DMTVKTSTTFANGT-----------------------SYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEA-VETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRY 1003
BLAST of NPHS1-3 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0111043) HSP 1 Score: 174.096 bits (440), Expect = 2.299e-43 Identity = 235/1113 (21.11%), Postives = 408/1113 (36.66%), Query Frame = 3
Query: 27 EKKMYQIWI----VIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLP-SIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGH---QILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQN--SNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNP-NEKSSFPPFIQQNHLDIISGIE---NEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKL-KRTLNSGFIECHAT----TENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQS------RLSHKILPEKYKPIVKNIKLKVLY-PPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWY---------FQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWL-----VPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
++ Q WI ++ L + I+ S+P+P Q Q F+ P D VL + C V N G VQW LG+ +PG PR ++GD +G +NL+I+ A D DY+CQV P + +R + L V P SI Y + D L+L C+ P W+ + E ++ E + + ++ S + C ++KA S A V + + G P I+ + + + +E C + G+P ++L W ++ + I++ Y RT R +SE+ + F N + N S NP + H+ ++ E + + + C T+ +KW++ + + TI +SP T + IT ++ KR+L + CH TEN + NV Y +ISGY+ + G + L C S GGNP TL WYKN+ + + I+ + + + DN + CEA + ++ +S L ++ V + I P ++ +C+SSSSNP + ++W ++G +T+ PG + G + + + + + N + CQS R +H+ + L VLY P F+ ++ EG ++ + Q ANP + W +R + +L +H +D+G Y N G AT + + V+Y + S NI + A C P+ V W +R+ Y D S ++ + S L I + D G ++C+ N + ++ L + P K + + L C P W +P + + +I T+ L + + +G Y+C A+NE G + + V L T QPD P S + + + + L+W G DGG + ++++R+
Sbjct: 38 RRRCRQHWIKHLQLLRLLAVIVLASAPVPSHAQQ-----------QKFRTTP-HDLQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHNSKVEVRENQD--------LQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKAL--------SPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSR-GGNPPAQLIW-------YKNGSQIRMAY-----------------RTSGR-----LSENIYT------FTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTI--VSP--------EGGWTTTSNITAVVEPNKRSL---VVICHGLNMQLTENVV---STHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRI--NSVIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIE-----PEELRPGMEATIICDSSSSNPPAKLSWWK--------------DGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEA-----------VSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAMLSCTVEGKPLTEEH-VKW------ERVGY----DMTVKTSTTFANGT-----------------------SYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEA-VETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRY 1003
BLAST of NPHS1-3 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 186.808 bits (473), Expect = 2.324e-47 Identity = 243/1060 (22.92%), Postives = 404/1060 (38.11%), Query Frame = 3
Query: 150 QCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQV-QPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIIN--IQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQN-SNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRV----NVSCISESPF-ITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLK-QKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNI-KLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQN---------RFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSN-FSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGT--DISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
Q FK P + V + + C V G VQW L+LG RS+PG PR + GD KG+++L+I ED G YECQV + + P++ R L+V S PY ++ E + + V+ C++ P F + E+ E + +Q + +++ ++ C KN A+ + E +T+ ++ P+I N+ V + ++ C M G+P + L W+ N G + + + + + + +K + H V +++ ++ SP +T++ + V P ++++ L +E E+IN+ C TSS ++++W L F + ++ + N + T K+ R N ++C A + + + V+Y + + +H G + L C S GGNP+ L W KNN +VL + + + + + + L + DN C++ ++ KV+ ++ F V+FP V ++I ++ +T + C + SSNP + I+W L +++G ++ G SKL+L L + CQ+ +L E V N L VL+PP F + + V E T L ++SANP W FQ R+ S L I N+ D G Y N GS +DV+YSP V S +F D+A+ C A NPI S+ FS W D + D +L+I Y + G YQC NGI G L + P K Q+ K + D S+ + + C W P TTE + L + V + + C A N G L + LL T PD P KL + + ++ L W+ G DGG Q F++R+
Sbjct: 27 QAFKTEP-RNVTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPY--FQLESEEPWVEGEQYTVT--------CIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASP----------RALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVC-MSYGGNPLATLHWTKN---GEVLSTSWDVDFVSRRASSVLKMEVKPEDN--HAVLQCQSINQVTRSPMSLTRT-----LTVLFEPAEVTL------------------------LGSFEAVEGEEINLSCSTSSSNPPVHIRWWL---GFKELNRTEVTFAE---------GDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKF---RVLFPAVSVKIVAKQ---KELRRGQTLDLDCLAGSSNPTANISWSL--------------GPVILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQA--FSNVLSEG----VNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGVLTI-YEVTRDRA---------------------------------GVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLD-IQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRY 957
BLAST of NPHS1-3 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 186.808 bits (473), Expect = 2.804e-47 Identity = 243/1060 (22.92%), Postives = 404/1060 (38.11%), Query Frame = 3
Query: 150 QCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQV-QPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIIN--IQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQN-SNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRV----NVSCISESPF-ITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLK-QKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNI-KLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQN---------RFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSN-FSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGT--DISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
Q FK P + V + + C V G VQW L+LG RS+PG PR + GD KG+++L+I ED G YECQV + + P++ R L+V S PY ++ E + + V+ C++ P F + E+ E + +Q + +++ ++ C KN A+ + E +T+ ++ P+I N+ V + ++ C M G+P + L W+ N G + + + + + + +K + H V +++ ++ SP +T++ + V P ++++ L +E E+IN+ C TSS ++++W L F + ++ + N + T K+ R N ++C A + + + V+Y + + +H G + L C S GGNP+ L W KNN +VL + + + + + + L + DN C++ ++ KV+ ++ F V+FP V ++I ++ +T + C + SSNP + I+W L +++G ++ G SKL+L L + CQ+ +L E V N L VL+PP F + + V E T L ++SANP W FQ R+ S L I N+ D G Y N GS +DV+YSP V S +F D+A+ C A NPI S+ FS W D + D +L+I Y + G YQC NGI G L + P K Q+ K + D S+ + + C W P TTE + L + V + + C A N G L + LL T PD P KL + + ++ L W+ G DGG Q F++R+
Sbjct: 72 QAFKTEP-RNVTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPY--FQLESEEPWVEGEQYTVT--------CIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASP----------RALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVC-MSYGGNPLATLHWTKN---GEVLSTSWDVDFVSRRASSVLKMEVKPEDN--HAVLQCQSINQVTRSPMSLTRT-----LTVLFEPAEVTL------------------------LGSFEAVEGEEINLSCSTSSSNPPVHIRWWL---GFKELNRTEVTFAE---------GDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKF---RVLFPAVSVKIVAKQ---KELRRGQTLDLDCLAGSSNPTANISWSL--------------GPVILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQA--FSNVLSEG----VNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGVLTI-YEVTRDRA---------------------------------GVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLD-IQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRY 1002
BLAST of NPHS1-3 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 144.05 bits (362), Expect = 3.825e-34 Identity = 168/694 (24.21%), Postives = 262/694 (37.75%), Query Frame = 3
Query: 1221 LDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLK-QKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNI-KLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQN---------RFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSN-FSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGT--DISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
L +E E+IN+ C TSS ++++W L F + ++ + N + T K+ R N ++C A + + + V+Y + + +H G + L C S GGNP+ L W KNN +VL + + + + + + L + DN C++ ++ KV+ ++ F V+FP V ++I ++ +T + C + SSNP + I+W L +++G ++ G SKL+L L + CQ+ +L E V N L VL+PP F + + V E T L ++SANP W FQ R+ S L I N+ D G Y N GS +DV+YSP V S +F D+A+ C A NPI S+ FS W D + D +L+I Y + G YQC NGI G L + P K Q+ K + D S+ + + C W P TTE + L + V + + C A N G L + LL T PD P KL + + ++ L W+ G DGG Q F++R+
Sbjct: 210 LGSFEAVEGEEINLSCSTSSSNPPVHIRWWL---GFKELNRTEVTFAE---------GDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKF---RVLFPAVSVKIVAKQ---KELRRGQTLDLDCLAGSSNPTANISWSL--------------GPVILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQA--FSNVLSEG----VNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGVLTI-YEVTRDRA---------------------------------GVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLD-IQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRY 830
BLAST of NPHS1-3 vs. Ensembl Zebrafish
Match: kirrel1b (kirre like nephrin family adhesion molecule 1b [Source:ZFIN;Acc:ZDB-GENE-070912-173]) HSP 1 Score: 68.9366 bits (167), Expect = 3.209e-11 Identity = 56/203 (27.59%), Postives = 79/203 (38.92%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWN---------SREVTTDEMEFIL--IINIQPNXXXXXXXXXXXXXGHQILCVIKNKATEL 752
D V+I + + +SCVV N G VQW L LG + PR +++ + G++NL+IT A D YECQ L R++ L V P P I E + ++ L CVS G P +W+ + S EV +D + IQP +G CV N A L
Sbjct: 33 DQSVVIGERVVLSCVVFNYTGIVQWTKDGLALGIGEDLRAWPRYRVLRIMDVGQYNLEITSADLTDDSLYECQATEAA---LRSRRAKLTVLIPPDGPVI----------EGSPEILLTAGTSFNLTCVSRGAKPMSTIEWYKDGIIVEGAHTSTEVLSDRKRVTTKSFLEIQP---------MDTDTGRNFTCVASNLAAPL 213
BLAST of NPHS1-3 vs. Ensembl Zebrafish
Match: kirrel1b (kirre like nephrin family adhesion molecule 1b [Source:ZFIN;Acc:ZDB-GENE-070912-173]) HSP 1 Score: 68.9366 bits (167), Expect = 3.615e-11 Identity = 56/203 (27.59%), Postives = 79/203 (38.92%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWN---------SREVTTDEMEFIL--IINIQPNXXXXXXXXXXXXXGHQILCVIKNKATEL 752
D V+I + + +SCVV N G VQW L LG + PR +++ + G++NL+IT A D YECQ L R++ L V P P I E + ++ L CVS G P +W+ + S EV +D + IQP +G CV N A L
Sbjct: 33 DQSVVIGERVVLSCVVFNYTGIVQWTKDGLALGIGEDLRAWPRYRVLRIMDVGQYNLEITSADLTDDSLYECQATEAA---LRSRRAKLTVLIPPDGPVI----------EGSPEILLTAGTSFNLTCVSRGAKPMSTIEWYKDGIIVEGAHTSTEVLSDRKRVTTKSFLEIQP---------MDTDTGRNFTCVASNLAAPL 213
BLAST of NPHS1-3 vs. Ensembl Xenopus
Match: NPHS1 (NPHS1 nephrin [Source:NCBI gene;Acc:100494620]) HSP 1 Score: 74.7146 bits (182), Expect = 9.960e-13 Identity = 105/466 (22.53%), Postives = 177/466 (37.98%), Query Frame = 3
Query: 1233 SGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKT-KFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFL--CESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNR----------FISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKV---VKTSINIFNDSAEFFCEASMNPIYSNF----SVVWRRI 2567
SG E ++ CFT++ + ++W L + V+ TI +S + N T++ R + + C A E + K + +V Y + I + G + +TC S GGNP P L W K+ + +I + K T + DN C A N+ P + + V FP + +N I + R + + C + SSNP S I+W ++ G + P Y G + S++ L K N + C++ S +L E KL +L+PP F+ + + E + ++ ANP +++W + + L I N+ DSG Y N G + +DV+YSP + T +++ +D AE C A NP + +VW R+
Sbjct: 335 SGPEKSNLTFSCFTAASNPPVQIRWWLGPKELVN----TIVTISDAVHGGKVTMSNLTMEAL--------REEHGMALTCEAFNEAILYTKSNSVTLSVQYPPQNVWIEAPPADKFFRAGTSVKMTCFSSGGNPPPRLTWIKDTKSLSGGSQIHSGK-IVTKEITIITVPSDNMATYRCNAS--NIGKTPALTASTKLKVQFPAID-----VNIISSAKEYRRGSTIILTCVTGSSNPASTISW-------VKDGEQLKAQDLGRK-------PSLYGGISSSSRVTLKAASKDNGRRVSCEAFSS--VLNEAVNTF---YKLNILFPPEFLDDQPAVVQAVEHGAALIPVRVRANPPQINYTWSLWGEKLIRDGAYRHHLRGEGALEIWNVSRADSGVYNVTCVNKEGKNSIVIRLDVQYSPTIRSLGDTEVDLGSD-AEIACTADANPATDSMFQWRWLVWIRL 760 HSP 2 Score: 71.633 bits (174), Expect = 8.800e-12 Identity = 45/133 (33.83%), Postives = 68/133 (51.13%), Query Frame = 3
Query: 78 IYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAI-SCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAH-PLLRRKSTLHVTKLPSIP 470
+Y S PL VT Q Q F+ P D ++ S A+ SC V + G VQW L+LG DR++PG R + GD KGE+NL+I +++ +D +Y CQV + ++ R + ++V P P
Sbjct: 6 LYVSLPLGVTAKQ-----------QVFRVQP--DNITVVQGSTALLSCEVEHGTGMVQWEKNGLLLGPDRNIPGFLRYSMTGDPHKGEYNLRIERSELDDDAEYHCQVGRSEVSLGIISRTALINVLIPPKAP 125 HSP 3 Score: 56.9954 bits (136), Expect = 2.387e-7 Identity = 56/195 (28.72%), Postives = 92/195 (47.18%), Query Frame = 3
Query: 1392 SKNHTIQTFITQKIKLKRTLNSGFIECHATTEN-QILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLV---LFSDE-IKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKL 1961
S++ T + K+ + + N + C A Q + NV + +I GY+ V G+ + L C S GNP TL+W KN+ +V +DE ++ + TL+ + DN V CEA + +V +K++ +I+NV+F P + I + P K +N T F C +++SNP I W L
Sbjct: 178 SRDKLENTAMVAKVIPQSSDNGKLLTCLALNPALQTPITVSFTMNVLFPPNAPIIEGYIGP-SVKAGETLKLICISLSGNPLATLQWSKNDDVVSTRWETDEVVRASRSHLTLN----IKPADNMAVVSCEAVN-HVTPETLKTS-IILNVVFLPTEVTILGSSSGPEK--SNLT--FSCFTAASNPPVQIRWWL 361
BLAST of NPHS1-3 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 69.3218 bits (168), Expect = 2.775e-11 Identity = 43/136 (31.62%), Postives = 58/136 (42.65%), Query Frame = 3
Query: 63 LGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIP 470
GS Y + PL A L + F + P +D ++ S+ + CVV G VQW L LG +R +PG R ++GD + GEHNL I + D YECQ R + L P IP
Sbjct: 7 FGSLSSYPTWPLTHLVPFSICAFFLTGFGAHFSQQP-SDKVIVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRARLTVLLPPGDPMIP 141
BLAST of NPHS1-3 vs. Ensembl Xenopus
Match: ptk7 (protein tyrosine kinase 7 (inactive) [Source:Xenbase;Acc:XB-GENE-5846944]) HSP 1 Score: 68.5514 bits (166), Expect = 5.623e-11 Identity = 80/341 (23.46%), Postives = 143/341 (41.94%), Query Frame = 3
Query: 2178 KNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWY--FQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGIN-----KNNKIYSK-----LIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILF 3164
K + + VLY P + + S + EGS L + ANP+ + +WY ++ ++ L I DSGSY+ +N++GS + + DV V S F DS ++ ++ + ++ +EP F+ S+ +SCN NS SN G++ KN K+ + +II +E G Y C N IG + + +N+ P ++++ ++ T+ L C + NP++ + + + EI TF G + + G+Y C+A+N G+ ++L F F E + L+ F
Sbjct: 334 KGVAINVLYAPTVTLKIPSREIREGSQYTLTCSVDANPQPVNITWYKDYEKILNKNRDTLTFPTIREQDSGSYRCKADNIIGSESSA---DVYGITCCVTVS---FRDSLVCRTDSLISDLNASLISFVFVVEPK-------KPSFVEGSSLRFSCNVNS---------SNPGVSQFTWYKNEKVMASERKNYIIIDTIKEEHHGNYMCKAGNKIGATSSQAVSINVLYPPKGVRVTPQAQIITEGDRIRL--ACSVDKSNPTVTEFRWYKDSALQPHRNKEI---TFPGI------KRTDSGKYTCEARNGIGNVTSQPITLNVQF---FMEKYSLWETF 638
BLAST of NPHS1-3 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 67.781 bits (164), Expect = 8.478e-11 Identity = 36/105 (34.29%), Postives = 49/105 (46.67%), Query Frame = 3
Query: 156 FKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIP 470
F + P +D ++ S+ + CVV G VQW L LG +R +PG R ++GD + GEHNL I + D YECQ R + L P IP
Sbjct: 30 FSQQP-SDKVIVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRARLTVLLPPGDPMIP 133
BLAST of NPHS1-3 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 67.781 bits (164), Expect = 8.556e-11 Identity = 36/105 (34.29%), Postives = 49/105 (46.67%), Query Frame = 3
Query: 156 FKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIP 470
F + P +D ++ S+ + CVV G VQW L LG +R +PG R ++GD + GEHNL I + D YECQ R + L P IP
Sbjct: 26 FSQQP-SDKVIVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRARLTVLLPPGDPMIP 129
BLAST of NPHS1-3 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 77.411 bits (189), Expect = 1.178e-13 Identity = 121/563 (21.49%), Postives = 197/563 (34.99%), Query Frame = 3
Query: 1587 GDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQ-----------TLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQY---NGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNI-----KLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS----------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVK----TSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSK--QGTDISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLF 3155
G + L C + GGNP P+L W K++ V + + + Q + + + + DN K C+A + S L++ FPP +L I L SS+ PG + L C SI N LS + G+ K QS ++ F+ H++ + ++ +L VLYPP F+ + ++ V E L +SANP F+W F+ +S S L + N+ D G Y+ +N G+A ++DV Y+P + T +N+ S + C NPI W R+ ++ D + + + T ++RI +KL S G YQC+ NG+ R + L + P + +K D + + L C N W VP + T + L + V + +KC A N GS + L+ +PD P K+
Sbjct: 472 GTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQLSA------STQLVVQ--FPPTNLTI------------------LANSSALRPGDALN---LTCVSISSNPPVN---------LSLDKEGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNV-GGSVDIVCTVDANPILPEM-FSWERLGEDEEELNLDDMEKM-----------SKGSTGRLRIRQ-----------AKL-------SQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDH-TNIQLVSISRPDPPLGLKVV 964 HSP 2 Score: 54.6842 bits (130), Expect = 1.001e-6 Identity = 29/86 (33.72%), Postives = 41/86 (47.67%), Query Frame = 3
Query: 186 VLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTL 443
V+ ++ + C V VQW L+LG + +PG PR + GD KGE +L I D +YECQV + P L S +
Sbjct: 57 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVI 142
BLAST of NPHS1-3 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 77.0258 bits (188), Expect = 1.591e-13 Identity = 121/563 (21.49%), Postives = 197/563 (34.99%), Query Frame = 3
Query: 1587 GDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQ-----------TLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQY---NGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNI-----KLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS----------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVK----TSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSK--QGTDISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLF 3155
G + L C + GGNP P+L W K++ V + + + Q + + + + DN K C+A + S L++ FPP +L I L SS+ PG + L C SI N LS + G+ K QS ++ F+ H++ + ++ +L VLYPP F+ + ++ V E L +SANP F+W F+ +S S L + N+ D G Y+ +N G+A ++DV Y+P + T +N+ S + C NPI W R+ ++ D + + + T ++RI +KL S G YQC+ NG+ R + L + P + +K D + + L C N W VP + T + L + V + +KC A N GS + L+ +PD P K+
Sbjct: 458 GTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQLSA------STQLVVQ--FPPTNLTI------------------LANSSALRPGDALN---LTCVSISSNPPVN---------LSLDKEGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNV-GGSVDIVCTVDANPILPEM-FSWERLGEDEEELNLDDMEKM-----------SKGSTGRLRIRQ-----------AKL-------SQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDH-TNIQLVSISRPDPPLGLKVV 950 HSP 2 Score: 54.6842 bits (130), Expect = 1.096e-6 Identity = 29/86 (33.72%), Postives = 41/86 (47.67%), Query Frame = 3
Query: 186 VLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTL 443
V+ ++ + C V VQW L+LG + +PG PR + GD KGE +L I D +YECQV + P L S +
Sbjct: 43 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVI 128
BLAST of NPHS1-3 vs. Ensembl Mouse
Match: Kirrel2 (kirre like nephrin family adhesion molecule 2 [Source:MGI Symbol;Acc:MGI:2442334]) HSP 1 Score: 69.707 bits (169), Expect = 1.908e-11 Identity = 62/242 (25.62%), Postives = 87/242 (35.95%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEG-GLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMK 899
D VL+ + + C + G VQW L LG +R +PG R I G+ G+H+L I + ED YECQ L R + LHV P P +L ++ L C S G P P WF + + D F HQ +K+KAT +E+ L T + T+I RS Q + D T S++
Sbjct: 29 DMVVLLGEEARLPCALGAYRGLVQWTKDGLALGGERDLPGWSRYWISGNSASGQHDLHIKPVELEDEASYECQASQAG---LRSRPAQLHVMVPPEAPQVL----------GGPSVSLVAGVPGNLTCRSRGDSRPAPELLWFRDG--IRLDGSSF-----------------------HQT--TLKDKATGTVENTLFLTPSSHDDGATLICRARS---------QALPTGRDTAVTLSLQ 221
BLAST of NPHS1-3 vs. Ensembl Mouse
Match: Ncam1 (neural cell adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:97281]) HSP 1 Score: 64.6994 bits (156), Expect = 9.193e-10 Identity = 85/405 (20.99%), Postives = 165/405 (40.74%), Query Frame = 3
Query: 2178 KNIKLKVLYPPFIM--NSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQN-------------LIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVV----KTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKP-WYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIR--QSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKG-ADGG-------------SEQTFQLRWWDEKQQSTFRYQTVKG 3284
K+I++ V PP + S+ + G +V L P+ T SW I +++ L I+N+ ND Y + EN G S + V PK+ +T++ + + CEAS +PI S+ WR N I+ + + ++P Q ++ + + + ++ S L + Q +D G Y C N IG + ++++L + P ++ Y+ +G ++ +TC ++ + +I W +++ S+N + I+ + +L + E+ FG Y C A N G L ++ L+ P P S + + + + + A GG E+++ +W+D K+ + T+ G
Sbjct: 160 KDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPT-MSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMEL-EEQVTLTCEASGDPIP---SITWRTSTRN--ISSEEKASW-------------TRPEKQETLDGHMVVRSHARV-SSLTLKSIQYTDAGEYICTASNTIG-QDSQSMYLEVQYAPKLQGPVAVYTWEGNQVN----ITCEVFAYPSATISWF--RDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFI-LVQADTPSSP-SIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEGIVTIMG 534
BLAST of NPHS1-3 vs. Ensembl Mouse
Match: Ncam2 (neural cell adhesion molecule 2 [Source:MGI Symbol;Acc:MGI:97282]) HSP 1 Score: 62.7734 bits (151), Expect = 3.378e-9 Identity = 84/365 (23.01%), Postives = 134/365 (36.71%), Query Frame = 3
Query: 2178 KNIKLKVLYPPFIMNSLNSL--YVFEGSTVNLQYQISANPKATHFSWYFQNRFI---------SSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKV--VKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYL--KISQYSKQGTDISHHSLLTCCFLSHVNPSIFW-----LVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGS 3212
++I + V PP IM S G + L + S +P T SW+ + I S L ++NI D GSY N G + + V P + +K N CEA P+ + W+R I + +S S P ++ + G S L I + SD G Y C + IG R ++HL+I P ++ + YS +G I+ ++C ++ SI W L+P N T++ + L + ++ FG+Y C A N G+ Y+ L P P K+ L + +S+ K G
Sbjct: 199 RDIIVIVNVPPAIMMPQKSFNATAERGEEMTLTCKASGSPDPT-ISWFRNGKLIEENEKYILKGSNTELTVRNIINKDGGSYVCKATNKAGEDQKQAFLQVFVQPHILQLKNETTSENGHVTLVCEAEGEPVP---EITWKRA----------------IDGVMFSEGDKS-PDGRIEVKGQHG-------RSSLHIRDVKLSDSGRYDCEAASRIGGHQR-SMHLDIEYAPKFVSNQTMYYSWEGNPIN----ISCDVTANPPASIHWRREKLLLPAKNTTHLKTHSVGRKM-----ILEIAPTSDNDFGRYNCTATNRIGTRFQEYILELADV-PSSPHGVKIIELSQTTAKISFNKPESHGG 524
BLAST of NPHS1-3 vs. UniProt/SwissProt
Match: sp|Q9U3P2|SYG2_CAEEL (Synaptogenesis protein syg-2 OS=Caenorhabditis elegans OX=6239 GN=syg-2 PE=1 SV=1) HSP 1 Score: 100.908 bits (250), Expect = 5.958e-20 Identity = 110/522 (21.07%), Postives = 218/522 (41.76%), Query Frame = 3
Query: 1728 RVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSW--------YFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTS---INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII------FSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
RV + N Q++ CEA + + ++S +L +V +PP + I + +++ ++ +C + SSNP + I+W+ + + V G +S N + NG+ ++ L+ + + ++ C + +H PE + + L V+YPP ++N ++ + EG + + NP + + W + R + L K + D+G Y N +GS + ++ V+YS ++ S I D+ CEA P +N + W Y++ IL+ + + + +R+N+ E G Y C NGIG+ L + P L +++Y+K + + C + P++ ++ ++I +S T+I ST+ L + + + Y + KC A+N G+ L+ + ++P PD P + + N I ++W DGGS+Q F++++
Sbjct: 546 RVDRTMNTQRLKCEATNAALDEPLIESQYL--SVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATD---------DNKVHLGDVSLNETTRDNGFNVENVLSFTPTEEYDGTVVHCIA--NH---PEWKHSVNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANM-IHW----------YKNGE----ILAAQHRGHKKAI----LRLNAT-------------------EQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKV--YAVPTVEFVWEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLS-IPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 1010 HSP 2 Score: 60.077 bits (144), Expect = 1.511e-7 Identity = 57/252 (22.62%), Postives = 100/252 (39.68%), Query Frame = 3
Query: 1227 IISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHA-TTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSI 1979
II+ EN +N+ C + + W ++DG +++S +K N T + + ++ R + I C A E I + T +V + + + + GD + + C GGNP P + WY N + + T +E + +Y N E ++ N + K + + V +PP +E+ + I AN C+S SNP S ITW ++N S+
Sbjct: 139 IIAVKENTPLNITCVVPNVKPEPEVLW------YMDG-----KVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKE--IKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFGESNIRYGSSAN----IQCKSLPSNPASQITW-IINGRSV 372
BLAST of NPHS1-3 vs. UniProt/SwissProt
Match: sp|Q9R044|NPHN_RAT (Nephrin OS=Rattus norvegicus OX=10116 GN=Nphs1 PE=1 SV=2) HSP 1 Score: 97.4413 bits (241), Expect = 6.520e-19 Identity = 136/592 (22.97%), Postives = 218/592 (36.82%), Query Frame = 3
Query: 1587 GDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQ-----------TLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQ----SRLSHKILPEKYKPIVKNI-KLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS----------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVK----TSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSK--QGTDISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLH------MYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
G + L C + GGNP P+L W+K++ V + + + Q + + + + DN K C+A + S L++ FPP +L I L SS+ PG + L C SI N S L + K QS ++ F+ R++ + E + V + + VLYPP F+ + ++ V E V L +SANP F+W F+ +S S L + N+ D G Y+ +N G+A ++DV Y+P + T +N+ S + C NPI W R+ + D L+++ + T ++RI +KL S G YQC+ NG+ R + L + P + + +K D + + L C N W VP + TE R G +H V + +KC A N GS + L+ +PD P K+ + +++ L W G DGG Q FQ+R+
Sbjct: 472 GTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAGQLSA------STQLVVQ--FPPTNLTI------------------LANSSALRPGDALN---LTCVSISSNPPVNLSWDKEGERL------EDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNV-GGSVDIVCTVDANPILPEM-FSWERL-------GEEEED----LNLDDMEKVSKGSTGRLRIRQ-----------AKL-------SQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLD---LQDPRYTEHRYHQ--GVVHSSLLTIANVSAAQDYALFKCTATNALGSDH-TNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRY 991
BLAST of NPHS1-3 vs. UniProt/SwissProt
Match: sp|O60500|NPHN_HUMAN (Nephrin OS=Homo sapiens OX=9606 GN=NPHS1 PE=1 SV=1) HSP 1 Score: 97.0561 bits (240), Expect = 8.371e-19 Identity = 127/578 (21.97%), Postives = 215/578 (37.20%), Query Frame = 3
Query: 1587 GDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLI----INVIFPPVHLEISCLNCIPNKIMANRTNEFL---CESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLY-PPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS----------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVK----TSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSK--QGTDISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW 3236
G + L C + GGNP P+L WYK++ V S + + L + + + + V S+N K+ L + V FPP ++ I A R + L C S SSNP ++W + ++G + + G + L ++ + + + C++ + E + + +L VLY P F+ + + E L +SANP F+W F+ +S S L + N+ D G Y+ +N G+A +DV Y+P + T +N+ S + C NPI W R+ ++ D + + + PT ++RI+ ++KL + G YQC+ NG+ R L L + P + +K D + + L C N W VP + T T + L + V + + C A N GS + + L+ +PD P K+ L +++ L W G DGG Q F +R+
Sbjct: 458 GTRVRLVCLAIGGNPEPSLMWYKDSRTVTES-RLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILA------NASALRPGDALNLTCVSVSSNPPVNLSW--------------DKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSA-----ELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNV-GGSVDIVCTVDANPILPGM-FNWERLGEDEEDQSLDDMEKI-----------SRGPTGRLRIH-----------HAKL-------AQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQ-TNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY 977 HSP 2 Score: 59.3066 bits (142), Expect = 2.881e-7 Identity = 28/75 (37.33%), Postives = 39/75 (52.00%), Query Frame = 3
Query: 201 SIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLL 425
S+ + C V+ VQW L+LG D +PG PR ++ GD +GE +L I D +YECQV + P L
Sbjct: 48 SVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPEL 122
BLAST of NPHS1-3 vs. UniProt/SwissProt
Match: sp|Q9QZS7|NPHN_MOUSE (Nephrin OS=Mus musculus OX=10090 GN=Nphs1 PE=1 SV=2) HSP 1 Score: 77.411 bits (189), Expect = 8.238e-13 Identity = 121/563 (21.49%), Postives = 197/563 (34.99%), Query Frame = 3
Query: 1587 GDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQ-----------TLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQY---NGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNI-----KLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS----------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVK----TSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSK--QGTDISHHSLLTCCFLSHVNPSIFWL---VPFSNEIIFSTNTTEIRQSTFCGFLHMY-VKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLF 3155
G + L C + GGNP P+L W K++ V + + + Q + + + + DN K C+A + S L++ FPP +L I L SS+ PG + L C SI N LS + G+ K QS ++ F+ H++ + ++ +L VLYPP F+ + ++ V E L +SANP F+W F+ +S S L + N+ D G Y+ +N G+A ++DV Y+P + T +N+ S + C NPI W R+ ++ D + + + T ++RI +KL S G YQC+ NG+ R + L + P + +K D + + L C N W VP + T + L + V + +KC A N GS + L+ +PD P K+
Sbjct: 472 GTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQLSA------STQLVVQ--FPPTNLTI------------------LANSSALRPGDALN---LTCVSISSNPPVN---------LSLDKEGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNV-GGSVDIVCTVDANPILPEM-FSWERLGEDEEELNLDDMEKM-----------SKGSTGRLRIRQ-----------AKL-------SQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDH-TNIQLVSISRPDPPLGLKVV 964 HSP 2 Score: 54.6842 bits (130), Expect = 7.001e-6 Identity = 29/86 (33.72%), Postives = 41/86 (47.67%), Query Frame = 3
Query: 186 VLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTL 443
V+ ++ + C V VQW L+LG + +PG PR + GD KGE +L I D +YECQV + P L S +
Sbjct: 57 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVI 142
BLAST of NPHS1-3 vs. UniProt/SwissProt
Match: sp|Q7TSU7|KIRR2_MOUSE (Kin of IRRE-like protein 2 OS=Mus musculus OX=10090 GN=Kirrel2 PE=1 SV=1) HSP 1 Score: 69.707 bits (169), Expect = 1.335e-10 Identity = 62/242 (25.62%), Postives = 87/242 (35.95%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEG-GLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMK 899
D VL+ + + C + G VQW L LG +R +PG R I G+ G+H+L I + ED YECQ L R + LHV P P +L ++ L C S G P P WF + + D F HQ +K+KAT +E+ L T + T+I RS Q + D T S++
Sbjct: 29 DMVVLLGEEARLPCALGAYRGLVQWTKDGLALGGERDLPGWSRYWISGNSASGQHDLHIKPVELEDEASYECQASQAG---LRSRPAQLHVMVPPEAPQVL----------GGPSVSLVAGVPGNLTCRSRGDSRPAPELLWFRDG--IRLDGSSF-----------------------HQT--TLKDKATGTVENTLFLTPSSHDDGATLICRARS---------QALPTGRDTAVTLSLQ 221
BLAST of NPHS1-3 vs. TrEMBL
Match: A0A0H3YJQ0 (NPHS1-3 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-3 PE=2 SV=1) HSP 1 Score: 2441.77 bits (6327), Expect = 0.000e+0 Identity = 1252/1252 (100.00%), Postives = 1252/1252 (100.00%), Query Frame = 3
Query: 36 MYQIWIVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVXXXXXXXXXXXXXXXXXXVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSEDIQMKLDPSSEPKELIIDQETIKF 3791
MYQIWIVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYKNEIPKNNEIIIDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNKSSLSLPLSLLKSGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSKLNLLLNYKQNNFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVNLENLNLSIYYIIIASSIVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSEDIQMKLDPSSEPKELIIDQETIKF
Sbjct: 1 MYQIWIVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYKNEIPKNNEIIIDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNKSSLSLPLSLLKSGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSKLNLLLNYKQNNFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVNLENLNLSIYYIIIASSIVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSEDIQMKLDPSSEPKELIIDQETIKF 1252
BLAST of NPHS1-3 vs. TrEMBL
Match: A0A0H3YFQ6 (NPHS1-6 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-6 PE=2 SV=1) HSP 1 Score: 407.142 bits (1045), Expect = 3.678e-115 Identity = 331/1188 (27.86%), Postives = 559/1188 (47.05%), Query Frame = 3
Query: 87 SSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPV-DAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLK--LICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQ------IVDENNDMEFTCSMKPTGHPKSKLKWSMNITA-----GFRMENL----IKIKYNE---NQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHL--DIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKL---DHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSI--EIGN---STNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLN-SLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS---------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSI---NIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNS---------NSKP-------------TNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII-----FSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGS-SRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNF-SIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKND 3431
SS + QS +C+ E+ N IQCF+EIP Y V + + C+V NQ+GKVQWRA ++LGYDR++PG P +++G V KGEH L+I+ +D+G YECQVQPV HPLLRRK+ L V P P I YK P +++II+ S+K+ ++ CVS+ G P+ FQWF N+ + +E L+ + ++S LSL S K G I C + N+ATE+ + + + +V +++ G P I + N +VD+N M TC P+G P L W R EN I +Y + +++++++ L + L H + C++ +P I+ S ++Y+ H P K+ I ++ S I ++ L ++I G E+ + C T YY +KW+ F + +SP+ K+ D +N TI KIK+ + + F++C A + + ++ + K ++ Y + I GY PV +TC + GGNP P L+W++++ + S + ++TL + +DN K FC+AKS ++K P+ S+ + + V+FPP+ + + L+ ++ + + C + SNP +++ WKL C + E+G T C+ ++ + S G++ G QS + ++K + + C+ + + E+++ + K I++ V + P +N + Y+ EG + L + ANP ++W+ + +S SK LII+N D +Y VV N G + + +++ Y P ++ I +AE C NP + S W ++ P S D +P +I CN N KP + + S C + ++ S LII +D G Y+C+ NGI + + L P L + ++SK ++ L C SH +P + W N + + N T R + L + + + G Y C+A+N +GS SR VS T PD P ++ ++ I +SWI+G DGGS QTFQ+RW ++ S +RY+ V + +K TL I+ S +Y I I + ++ ++ I +S N+
Sbjct: 43 SSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIIN---SIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENS-ALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRS---LSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIM--TCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISV--------SDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWV-----FSPNPAINNDFISPVEKIWKEDESFRRNSTI------KIKINKQFDQSFLDCQAWSGIK-SVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGA--EVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCE--VFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTI--GCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTST--PDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNE 1193
BLAST of NPHS1-3 vs. TrEMBL
Match: G7YEL7 (Nephrin OS=Clonorchis sinensis OX=79923 GN=CLF_106049 PE=4 SV=1) HSP 1 Score: 394.045 bits (1011), Expect = 2.424e-110 Identity = 323/1239 (26.07%), Postives = 542/1239 (43.74%), Query Frame = 3
Query: 51 IVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDA--HPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTD----------------EMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVD--ENNDMEFTCSMKPTGHPKSKLKW--------------------------SMNITAGFR--------------------MENLIK-----IKYNENQ--DELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNC------SSIEIGNST--------------NCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKN-IKLKVLYPP--FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSG-----------------SYKFVVENLLGSATRSFEIDVKYSPKVVKTS----INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCN-SNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFS--------------TNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW-WDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSI 3368
+V V+ ++ S V QS C+ +E ++ IQCF +P Y V + +++ + CVV NQHGKVQWRAK ++LGYDRS+PG PR +IIGDV +G+H L+I Q ED+G+YECQV PV A HPLLRRK+ L V PS+P ILY + P +N++II + + + ++CV+ GG P P F W N++E+ T +++ I+ + +S LSL + LK G Q+ C + N AT+L Q + +V + + G P I + + E +++ C P G+P L W MN + M +LI + Y N+ D+LI L++ ++ R +H + C + P + +I+VK+ P VT + E + E+ + ++ G E ++C TS Y+ N+KWI Q D G + L K+ +Q +K+ + G+++C +E + + + + ++ Y + VI+GY + PV + +TC + GGNP P L W+++ + S+E+ + +L + DNG C A++ + + S +++NV+FPP + ++ P+ + + + C++ SSNP +II W C S + +T C S ++GT PG G K QS+L L + + ++C++ PE P+ + ++L + + P + + + EG+TVNL +NP T SW+ + R I + + SG +Y V N + ++ F ++V + + + +D C+A+ NP + S W R P +D + LS CN S++ P+ + +C + + + S+ I D G Y C NG+G R+N+H P +++ +Y+K + LTC + P I WL + +II T IR + L + +++ FG Y+CQA N +G + L VSL T PD P + +L N++ ++W +G DGG QTFQ+RW + F+Y V N + +E I+ G+ + VNS+
Sbjct: 25 LVFVVTIGLLQVSDAARVIQSSACTPQERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIISQPDPIVR-ITVLCVTVGGSPPPSFTWIINNQEIPTKPLQTSAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCGVTNAATQLHHDIQQ---RNLTTQVMIRVHTPPGPPRIISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPAQVGDQKTGRIIGFGGRGGARMNEYSAVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPAN---VTISVEGGSVREE-----LVSGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSD--SGPMKPPRQLFGQTKIMRTPDGKSWLQESRV-DVKISDEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRNLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNSEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASEAVVLNVVFPPQGVSVNFHG--PSVVPSGQPLVISCQADSSNPVAIINWWHFRCGPAHAFSQFKASKTTVDEVLYERRTPERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTE-----NPEYGPPVWHDRLQLNITFAPQFLGLQTRKEHVIREGTTVNLDLGPYSNPPVTSVSWFVEGRPIPPETAPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAIFFLNVTFPASLAGAAEENVTQTTSDQVTLVCQATANPSIAIQSFTWYRYVPPPGWP-QDVNEAEVQLSAPMPCNQSDAGPSVDDKYFIHCQRDDSVHMTSRFSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFAPGIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLK--AGQIIHDSQREGSGASTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLE-VSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGA-EYQVSVNSM 1236
BLAST of NPHS1-3 vs. TrEMBL
Match: A0A4S2LBK4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 393.275 bits (1009), Expect = 4.122e-110 Identity = 315/1222 (25.78%), Postives = 537/1222 (43.94%), Query Frame = 3
Query: 102 VTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDA--HPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEF----------------ILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVD--ENNDMEFTCSMKPTGHPKSKL--KWSMNIT----------------AGFRM---------------------------------ENLIKIKY--NENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNC-------------SSIE-------IGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKN-IKLKVLYPP--FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSG-----------------SYKFVVENLLGSATRSFEIDVKYSPKVVKTS----INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCN-SNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFS--------------TNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW-WDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSI 3368
V QS C+ RE ++ IQCF +P Y V + +++ + CVV NQHGKVQWRAK ++LGYDRS+PG PR +IIGDV +G+H L+I Q ED+G+YECQV PV A HPLLRRK+ L V PS+P ILY + P +N++II + + + ++C + GG P P F W N++E+ ++ I+ + +S LSL + LK G Q+ C + N AT+L Q + +V + + G P I + + E +++ C P G+P L +W + + G RM E++ + Y N+ +D+LI L++ ++ R +H + C + P + +I+VK+ P VT + + + E+ + ++ G E ++C TS Y+ N+KWI Q D G + L K+ +Q +K+ + G+++C +E + + + + ++ Y + VI+GY S PV + +TC + GGNP P L W+++ + ++E+ + +L + DNG C A++ + + S+ +++NV+FPP + ++ P+ + + + C++ SSNP + I W C ++IE C S ++GT PG G K QS+L L + + ++C++ PE P+ + ++L + + P + + + EG+TVNL +NP T SW+ + R I + + SG +Y V N + ++ F ++V + + + +D C+A+ NP + + W R P +D + LS CN S++ P+ + +C + + + S+L I D G Y C NG+G R+N+H P +++ +Y+K + LTC + P I WL + ++I+ T IR + L + +++ FG Y+CQA N +G + L VSL T PD P + +L N++ ++W +G DGG QTFQ+RW + F+Y V N + +E I+ G+ + VNS+
Sbjct: 43 VIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIISQPDPIVR-ITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQ---RNLTTQVVIRVHTPPGPPRIISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPAN---VTISVDGGSVREE-----LVSGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSD--SGPMKPPRQLFGQTKIMRTPDGKSWLQESRV-DVKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVNFHG--PSVVPSGQPLVISCQADSSNPVATINWWHFRCGPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTE-----NPEYGPPVWHDRLQLNITFAPQFLGLQTRKEQVIREGTTVNLDLGPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVTLVCQATANPSIATQAFTWYRYVPPPGWP-QDVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRDDSIHMTSRLSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLK--AGQMIYDSQREGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLE-VSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGA-EYQVSVNSM 1237
BLAST of NPHS1-3 vs. TrEMBL
Match: A0A4S2LBR4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 393.66 bits (1010), Expect = 4.398e-110 Identity = 315/1222 (25.78%), Postives = 538/1222 (44.03%), Query Frame = 3
Query: 102 VTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDA--HPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEF----------------ILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVD--ENNDMEFTCSMKPTGHPKSKL--KWSMNIT----------------AGFRM---------------------------------ENLIKIKY--NENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNC-------------SSIE-------IGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKN-IKLKVLYPP--FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSK-----------------QNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTS----INIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCN-SNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFS--------------TNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW-WDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSI 3368
V QS C+ RE ++ IQCF +P Y V + +++ + CVV NQHGKVQWRAK ++LGYDRS+PG PR +IIGDV +G+H L+I Q ED+G+YECQV PV A HPLLRRK+ L V PS+P ILY + P +N++II + + + ++C + GG P P F W N++E+ ++ I+ + +S LSL + LK G Q+ C + N AT+L Q + +V + + G P I + + E +++ C P G+P L +W + + G RM E++ + Y N+ +D+LI L++ ++ R +H + C + P + +I+VK+ P VT + + + E+ + ++ G E ++C TS Y+ N+KWI Q D G + L K+ +Q +K+ + G+++C +E + + + + ++ Y + VI+GY S PV + +TC + GGNP P L W+++ + ++E+ + +L + DNG C A++ + + S+ +++NV+FPP + ++ P+ + + + C++ SSNP + I W C ++IE C S ++GT PG G K QS+L L + + ++C++ PE P+ + ++L + + P + + + EG+TVNL +NP T SW+ + R I + + L + I +Y V N + ++ F ++V + + + +D C+A+ NP + + W R P +D + LS CN S++ P+ + +C + + + S+L I D G Y C NG+G R+N+H P +++ +Y+K + LTC + P I WL + ++I+ T IR + L + +++ FG Y+CQA N +G + L VSL T PD P + +L N++ ++W +G DGG QTFQ+RW + F+Y V N + +E I+ G+ + VNS+
Sbjct: 118 VIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIISQPDPIVR-ITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQ---RNLTTQVVIRVHTPPGPPRIISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPAN---VTISVDGGSVREE-----LVSGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSD--SGPMKPPRQLFGQTKIMRTPDGKSWLQESRVD-VKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVNFHG--PSVVPSGQPLVISCQADSSNPVATINWWHFRCGPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTE-----NPEYGPPVWHDRLQLNITFAPQFLGLQTRKEQVIREGTTVNLDLGPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVTLVCQATANPSIATQAFTWYRYVPPPGWP-QDVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRDDSIHMTSRLSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLK--AGQMIYDSQREGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLE-VSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGA-EYQVSVNSM 1312
BLAST of NPHS1-3 vs. Ensembl Cavefish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 102.834 bits (255), Expect = 1.423e-21 Identity = 113/462 (24.46%), Postives = 186/462 (40.26%), Query Frame = 3
Query: 1221 LDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTK-FNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQN----------LIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSN-FSVVW 2558
L +E + + C TSS ++++W L F + + T+ I +N + T + R NS + C A K++ +V+Y + + + G + L C S GGNP+ L W KNN V + + + +FL + DN C+A +E + ++S + + V FP V ++I ++ A R+ C + SSNP + I+W L ST + G ++ G SKL L L + + + CQ+ S +L E L VL+PP F + + + + E T L +SANP+ W F + ++ L I N+ D+G+Y+ N G + ++DV+YSP V + +F D+A+ CEA NPI FS W
Sbjct: 359 LGSFEAVEGNQVTLCCHTSSSNPPVHIRWWL---GFKELNNATVTISE---------GENGGMMTMSNLTYTVSREENSLPLTCEAFNRGTRYSSIKSQPLSVYYPPQKVWLDAPPEGTLLRSGTTVRLVCFSSGGNPTGRLTWMKNNKTVQVPSKHVESERGVSREFLLTLQPSDNLATYRCDATNEAKE---IRSAQIKLLVQFPAVSVKILVKQ---KELRAGRSLSLDCLAGSSNPKANISWSL--------------GSTRLNGVEQAPKRAEFGGLSVSSKLTLPLGSEHHGKRVSCQAFSS--LLSEGVNTF---YVLNVLFPPEFAEDQPSEVQIVEDDTATLPIMVSANPEDFTCEWLFHGEKVVKDRDHRYHFPEGWALEIWNVTRRDAGNYRVECSNAEGKRSSIIKLDVQYSPTVKMKTDPVFVNVGDTADLMCEADANPITPGMFSWKW 783 HSP 2 Score: 68.9366 bits (167), Expect = 3.736e-11 Identity = 38/109 (34.86%), Postives = 57/109 (52.29%), Query Frame = 3
Query: 150 QCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQV-QPVDAHPLLRRKSTLHVTKLPSIPY 473
Q F+ P + V + + C V G VQW L+LG RS+PG PR + GD +G+++L+I + ED YECQV + + P++ R L+V P+ PY
Sbjct: 43 QAFRTEP-RNLTVRTGATALLKCEVLRASGSVQWVKDGLLLGPQRSLPGHPRYSMTGDQDRGQYHLQIEDVRLEDDSPYECQVGRSKTSRPIVSRTVWLNVQIPPAEPY 150 HSP 3 Score: 58.151 bits (139), Expect = 6.183e-8 Identity = 50/161 (31.06%), Postives = 74/161 (45.96%), Query Frame = 3
Query: 1548 VISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITW----KLLNCSSIEIGNSTNCSSTVM 2018
VI G SQ V G + L C SYGGNP TL W KN ++ + E+ ++ + +V DNG + CE+ ++ V P +S + V+F P E++ L + N+ C +SSSNP I W K LN +++ I N M
Sbjct: 259 VIEGLRSQ-EVKAGTSLRLVCLSYGGNPLATLHWTKNGEVLSTNWEVDVVSQKASSGLNMQVQPEDNGAVLCCESVNQ-VSRSP-QSITRTLTVLFEPA--EVTLLGSF-EAVEGNQVT-LCCHTSSSNPPVHIRWWLGFKELNNATVTISEGENGGMMTM 412
BLAST of NPHS1-3 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 65.0846 bits (157), Expect = 4.116e-10 Identity = 34/100 (34.00%), Postives = 48/100 (48.00%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYI 476
D V+ S+ + CV+ G VQW L LG +R +PG R ++GD GEH+L I ++ D YECQ L ++ L V PS P +
Sbjct: 21 DQVVVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQATQAG---LRSHRARLTVLVPPSDPVV 117
BLAST of NPHS1-3 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 65.0846 bits (157), Expect = 4.232e-10 Identity = 34/100 (34.00%), Postives = 48/100 (48.00%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYI 476
D V+ S+ + CV+ G VQW L LG +R +PG R ++GD GEH+L I ++ D YECQ L ++ L V PS P +
Sbjct: 24 DQVVVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQATQAG---LRSHRARLTVLVPPSDPVV 120
BLAST of NPHS1-3 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 65.0846 bits (157), Expect = 4.718e-10 Identity = 34/100 (34.00%), Postives = 48/100 (48.00%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYI 476
D V+ S+ + CV+ G VQW L LG +R +PG R ++GD GEH+L I ++ D YECQ L ++ L V PS P +
Sbjct: 59 DQVVVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQATQAG---LRSHRARLTVLVPPSDPVV 155
BLAST of NPHS1-3 vs. Ensembl Cavefish
Match: kirrel3a (kirre like nephrin family adhesion molecule 3a [Source:ZFIN;Acc:ZDB-GENE-091117-44]) HSP 1 Score: 61.6178 bits (148), Expect = 5.561e-9 Identity = 67/257 (26.07%), Postives = 105/257 (40.86%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXX--XXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNS--NQIVDENNDMEFTCSMKPTGHPKSKLKWS 935
D V+ + + C + HG V W L LG +R + G PR +IGD +KGE++L I + + D G +EC Q + A + R + L V P P I+ ++ L L C ++ P W N + L+ + Q + +L S ++SG +I C NKA EA VT+ + PL+ S Q + E N ++F CS K P + +W+
Sbjct: 37 DVVVVAGQPVTLPCSITGFHGMVLW--DGLALGVNRDLSGYPRYDVIGDHSKGEYHLLIQRTELGDDGSFEC--QGIQAG-IRSRPARLTVLVPPDDPVIIGAPI----------VSLRAGDPLNLTCHADNAKPAASIIWIRNGEVLNGAMYSKTLLRDGQRESTVSTLYISASNIESGQRITCRASNKAAP----------NGKEATVTIDI---QHLPLVNLSVEPQPILEGNLVKFHCSAK-ANPPVTHYRWA 264
BLAST of NPHS1-3 vs. Ensembl Sea Lamprey
Match: ncam1b (neural cell adhesion molecule 1b [Source:ZFIN;Acc:ZDB-GENE-010822-2]) HSP 1 Score: 57.3806 bits (137), Expect = 2.488e-8 Identity = 46/174 (26.44%), Postives = 75/174 (43.10%), Query Frame = 3
Query: 2346 NLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRE--NLHLNIPIKPWYLKIS 2852
+L ++++ + D+G Y + N +G A+ + +DV Y PK + N++ CE NP +V W SRD PI S N TN + ++S G +S L I+P +SDFG Y C+ N I + +E + +P P +K +
Sbjct: 256 SLTLRDVQYTDAGEYMCIANNNVGQASAAMHLDVAYGPKFAGEKVTYHSWPNNAVNVTCEVLANP---KAAVSW-------------SRDGQPIPSANL--------TNVI-VHSFPG-------FSVLEITPESQSDFGSYNCVAVNNIASESKEFVLVEAALPSAPQSVKAT 397 HSP 2 Score: 51.6026 bits (122), Expect = 1.450e-6 Identity = 51/235 (21.70%), Postives = 95/235 (40.43%), Query Frame = 3
Query: 2505 FFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSL------LTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGF--LHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLS 3185
CE+ P+ + + WRR+ +D+ +R + L ++ + S + S L + Q +D G Y C+ N +G + +HL++ P + G +++HS +TC L++ ++ W + I S N T + +F GF L + + +S FG Y C A N S +V L+ P P+S K ++ + ++
Sbjct: 212 LTCESEGEPLPT---ITWRRV--SDQHVFRQGQQTL---------------EGRIEVRSYAKV-------SSLTLRDVQYTDAGEYMCIANNNVG-QASAAMHLDVAYGPKFA--------GEKVTYHSWPNNAVNVTCEVLANPKAAVSWS--RDGQPIPSANLTNVIVHSFPGFSVLEITPESQSDFGSYNCVAVNNIASESKEFV-LVEAALPSAPQSVKATAVYSTSAEIT 407
BLAST of NPHS1-3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008186.1 (pep scaffold:Pmarinus_7.0:GL476501:83598:168579:1 gene:ENSPMAG00000007342.1 transcript:ENSPMAT00000008186.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 50.0618 bits (118), Expect = 4.128e-6 Identity = 86/389 (22.11%), Postives = 144/389 (37.02%), Query Frame = 3
Query: 2172 IVKNIKLKVLYPPFIMNSLNS--------LYVFEGSTVNLQYQISANPKATHFSWYFQN-RFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVV---KTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFW------LVPFS--NEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQT-FQLRW------WDEKQQS 3257
++ +K L PF M ++ + V S + + + P + FISS L+I N+ +G+Y + N + + ++ V+ P+ V K IF C A PI ++W+ + N + F P+LS +P L++ QE D G+Y C NG+G +++ LN+ I L SQ + HS L C I W + P ++++ +T + ST + V+ +S F + C A N G R + L PD PE ++ + I L W G DG S T F + + W++KQ++
Sbjct: 422 LIVTVKEPPLIQPFEMPAVTQGQRVYVPCVIVSGDSPIRITWHKDGQPLPPRLGAQVETTEFISS---LLIPNVTQLHNGNYTCLARNEAATVSHGSQLIVRVPPRFVVQPKDQDGIFGKPVVLNCSADGYPIPK---IIWKYSKGNG------NPQFQPVLS----GGGRFQPLGN----------------GSLLLRQVQEEDGGFYLCQVSNGVGTDISKSMLLNVKIP--ALVTSQANVTLATRGQHSNLQCSARGERPIIIRWEKEETLIDPERMFRYVVYTQDTGDELLSTL--QILPTVRGDSGF--FTCHAINSYGEDR-GLIQLTVQEPPDPPE-VEVREVNARTITLRWTSGFDGNSPITAFDIEYKNRSDTWEQKQRT 770
BLAST of NPHS1-3 vs. Ensembl Nematostella
Match: EDO45383 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RT26]) HSP 1 Score: 56.6102 bits (135), Expect = 4.241e-8 Identity = 78/309 (25.24%), Postives = 125/309 (40.45%), Query Frame = 3
Query: 2247 EGSTVNLQYQISANPKATHFSWY---------FQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFND-------SAEFFCEASMNPIYS-----NFSVVWRRIEPNDRIAYRDSRDFLPILSIN------YSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMK--GRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFW------LVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQA 3068
EG V + Q + NP + + +W RF S+ L+I N+ DSG+Y N LG + +DV+Y P+ V N+ D S F C NP + N S + ++P + + L I ++N Y CN++S G + S L+IS +D Y C N G K G NL +N P P +L+ S ++ + + + LTC ++ W L P S II ++ + +ST ++++ +E+Y YKC A
Sbjct: 10 EGQVVKFECQTTGNP-SPNVTWLKSGSAVNTSVDPRFYSNDTILMITNVSRIDSGTYSCNASNELGIISAEAALDVRYGPEFV----NVPQDKTPNEGWSVTFECHTKGNPPPTVTWLKNGSALNTSVDPR----FYSNGTHLMITNVNRTDSGAYRCNASSI----------LGPENDGNETSFLMISNLNRTDEASYTCRAKNAAGEKTSGSVNLAVNYP--PKFLQ-SLKDEETLNETDNLTLTCKLEGKPLATVVWLKDNTELEPDSRLIITQPSSVDSGESTLT-IINVHRHDEAY---YKCLA 292
BLAST of NPHS1-3 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 167.548 bits (423), Expect = 1.626e-41 Identity = 232/1073 (21.62%), Postives = 385/1073 (35.88%), Query Frame = 3
Query: 150 QCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVD-AHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNP-NEKSSFPPFIQQNHLDI---ISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQK-TKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFL---CESSSSNPGSIITWKLLNCS-SIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKN-----IKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFI---SSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGT--DISHHSLLTC----------------CFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW-WDE 3245
Q F+ P + V + + + C V G VQW L+LG RS+PG PR +IG +G+++L+I AQ ED YECQ D + ++ L V PS PY D + CV+ P+ E F+ I +S + +G + C KN A L + +T VT+ ++ P I N ++ M + +G+P + L+WS N ++ + ++ + +LK + I N +LS +FN S +P + F L + + IE +++ + CF +S ++++W L + + + T+ I N + T + R N + C A + K K K +V+Y + + P++ G + L C S GGNP+PTL W KN V + + + + + DN C+A + K I + L V F V ++I+ + R E L C + SSNP + I W + + + + + TL N Q++ ++ + Y P+++ +L VL+PP F + V E + +SANP+ W + + +L I+N D+G Y EN G + + +DV Y+ V +F +A+ C A NPI S+ W+ +E +R+ G S L I + G YQC NGIG+ G N+ L + KP K Q+ K + D S + L C FL NP + E F T+T + + V + + C A+N G ++ + L+ T PD P F+ +++ L WI G +GG +Q F++R+ WD+
Sbjct: 50 QAFRIEP-RNLTVRMGATAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIGK--RGQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNPYFTMDMAAYWVAGESYD----------VTCVAPDAKPEA--------------EHFFVCIFTFGSCVTSERVTALSSDNGRLLACHAKNAA---LSKPVVTT-------VTMNVYYPPDAPFIVNLDRDEVRAGRMLMLECVSGSGNPLAALRWSKN-------GEIVSTTWEADEFARKSRSVLKMV--------------------------ITPADNQAELSCESFNAVSLSPLSITRKLDVFFDPEELTLSGSLEAIEGQEVVLSCFAASSNPPVDIRWWLGYKELNN----TVFIREE--------GDNGGMTTVSNVTYRFSREDNGLMLNCEAFNKGTRFSKIKDAKISVYYPPQKVWLDAPAQDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAARELHLVLTPSDNMATYRCDATNNAKKTISAHAKLL---VYFTAVSMKITAHQEVL------RQGEVLTLECLAESSNPTANIFWSVGAVRFGVTLSTKHQSGAVTVNSTLLLNLTSQHH---------------------------KQNVICQAYNPVLEEGANTYYRLNVLHPPEFSSAQPTEIQVEEDEMATIPLLVSANPEGVSCVWLHRRETLVKGDPDGSLEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVTAEKDPVFVNLGGTADLICVADANPIISDM-FSWKWLE-GERV--------------------------------EMGEETQEDESSLLTIRNVTRAHSGLYQCTANNGIGLSGSVNMQLVVQFKPELQKGPQWRKVASRGDGSTTAELVCRAEGIPRIDFSWEKKGVFLDLANPR--YEEKTVREGSFHTSTLRV----------VNVSAVMDYAVFSCYARNSLGEDKVD-IQLVSTNHPDPPSMFRHVSSTHDSVTLEWIPGFNGGLQQKFRIRYQWDQ 957
BLAST of NPHS1-3 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 160.229 bits (404), Expect = 2.370e-39 Identity = 250/1125 (22.22%), Postives = 413/1125 (36.71%), Query Frame = 3
Query: 150 QCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVD-AHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIIN--IQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNP-NEKSSFPPFIQQNHLDI---ISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQK-TKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFL---CESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHK--ILPEKYKPIVKN-----IKLKVLYPP-FIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNL----------IIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIF---NDSAEFFCEASMNPIYSN-FSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGT--DISHHSLLTC----------------CFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRW-WDEKQQSTFRYQ----------TVKGNQSKETLEITISNFSIGS 3338
Q F+ P + V + + + C V G VQW L+LG RS+PG PR +IG +G+++L+I AQ ED YECQ D + ++ L V PS PY D + CV+ P+ + + ++T E + + + +++ +G + C KN A L + +T VT+ ++ P I N ++ M + +G+P + L+WS N ++ + ++ F R + S + I N +LS +FN S +P + F L + + IE +++ + CF +S ++++W L + + + T+ I N + T + R N + C A + K K K +V+Y + + P++ G + L C S GGNP+PTL W KN V + + + + + DN C+A + K I + L V F V ++I+ + R E L C + SSNP + I W + ++ + S G ++ N + +L S+ HK ++ + Y P+++ +L VL+PP F + V E + +SANP+ W + + NL I+N D+G Y EN G + + +DV Y+ V +F +A+ C A NPI S+ FS W E + D +L+I N R +S G YQC NGIG+ G N+ L + KP K Q+ K + D S + L C FL NP + E F T+T + + V + + C A+N G ++ + L+ T PD P F+ +++ L WI G +GG +Q F++R+ WD Q +F+Y TV G Q +T+ NFSI +
Sbjct: 27 QAFRIEP-RNLTVRMGATAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIGK--RGQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNPYFTMDMAAYWVAGESYD----------VTCVAPDAKPEAEVILYKDGVKLTGVESSSVSGSKDKLLNTLAEVTVTALSSDNGRLLACHAKNAA---LSKPVVTT-------VTMNVYYPPDAPFIVNLDRDEVRAGRMLMLECVSGSGNPLAALRWSKN-------GEIVSTTWEADE---------------FARKSRSVLK-----------MVITPADNQAELSCESFNAVSLSPLSITRKLDVFFDPEELTLSGSLEAIEGQEVVLSCFAASSNPPVDIRWWLGYKELNN----TVFIREE--------GDNGGMTTVSNVTYRFSREDNGLMLNCEAFNKGTRFSKIKDAKISVYYPPQKVWLDAPAQDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAARELHLVLTPSDNMATYRCDATNNAKKTISAHAKLL---VYFTAVSMKITAHQEVL------RQGEVLTLECLAESSNPTANIFWSV---GAVRFQKAEQTHSKDKSGAVTVN----------------------STLLLNLTSQ-HHKQNVICQAYNPVLEEGANTYYRLNVLHPPEFSSAQPTEIQVEEDEMATIPLLVSANPEGVSCVWLHRRETLVKGNNLHHYWLEDGSLEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVTAEKDPVFVNLGGTADLICVADANPIISDMFSWKWLGEE-EVEMGEETQEDESSLLTIR----------NVTRAHS------------------------GLYQCTANNGIGLSGSVNMQLVVQFKPELQKGPQWRKVASRGDGSTTAELVCRAEGIPRIDFSWEKKGVFLDLANPR--YEEKTVREGSFHTSTLRV----------VNVSAVMDYAVFSCYARNSLGEDKVD-IQLVSTNHPDPPSMFRHVSSTHDSVTLEWIPGFNGGLQQKFRIRYQWD--QSVSFQYVDVFPPDATTFTVTGLQP-----VTVYNFSINA 993
BLAST of NPHS1-3 vs. Ensembl Medaka
Match: ENSORLT00000046072.1 (pep primary_assembly:ASM223467v1:1:2157820:2166779:1 gene:ENSORLG00000025208.1 transcript:ENSORLT00000046072.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.0846 bits (157), Expect = 3.170e-10 Identity = 73/290 (25.17%), Postives = 117/290 (40.34%), Query Frame = 3
Query: 1578 VHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFI--SSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEI 2441
+ EG + LTC S NP+ RWYKNN +L D+ + R + + +K C A++ K+ S+++ INV +PP E S ++ P+ + ++ L SS +NP + TW N + V ++ + G Y+ C+S H P++ L V YPP I + + +GS+V L ANP A +FSW+ ++ +S N I ++ SG Y EN GS S +
Sbjct: 144 IMEGSSVTLTC-SCDANPAAKFRWYKNNQTLLLEDQ----------SLILRSVQRSDSRKFHCVAEN---KLGEAASDYVFINVEYPP---ETSSVSVSPSAEVLEGSSVTLTCSSDANPAANYTWFKDNRPLLS-------EDKVHFSSIRSEHSGNYS----------------------CKSENKHG--QSSSTPLL----LDVQYPPRIPSVSVFGKISKGSSVTLTCSSDANP-AANFSWFKKDEDSPKASGPNWTISDLRAEHSGFYYCEAENRRGSLNSSLHL 380 HSP 2 Score: 54.299 bits (129), Expect = 7.107e-7 Identity = 45/149 (30.20%), Postives = 72/149 (48.32%), Query Frame = 3
Query: 2241 VFEGSTVNLQYQISANPKATHFSWYFQNR-FISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFE-IDVKYSPKVVKTSIN-----IFNDSAEFFCEASMNPIYSNFSVVW----RRIEPNDRIAYRDSRDFLPILSINYSCNSNSK 2654
+ EGS+V L ANP A F WY N+ + Q+LI++++ +DS + V EN LG A + I+V+Y P+ S++ + S C + NP +N++ W R + D++ + R S NYSC S +K
Sbjct: 144 IMEGSSVTLTCSCDANP-AAKFRWYKNNQTLLLEDQSLILRSVQRSDSRKFHCVAENKLGEAASDYVFINVEYPPETSSVSVSPSAEVLEGSSVTLTCSSDANPA-ANYT--WFKDNRPLLSEDKVHFSSIRS---EHSGNYSCKSENK 285
BLAST of NPHS1-3 vs. Ensembl Medaka
Match: ENSORLT00000038190.1 (pep primary_assembly:ASM223467v1:1:2022690:2038485:-1 gene:ENSORLG00000030207.1 transcript:ENSORLT00000038190.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 64.3142 bits (155), Expect = 3.863e-10 Identity = 74/290 (25.52%), Postives = 115/290 (39.66%), Query Frame = 3
Query: 1578 VHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFI--SSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEI 2441
+ EG + LTC S NP+ RWYKNN +L D + R + + K C A++E + S+++ INV +PP E S ++ P+ + ++ L SS +NP + TW N + V ++ + G Y+ C+S H P++ L V YPP I + + +GS+V L ANP A +FSWY ++ +S N I + SG Y EN GS S ++
Sbjct: 148 IMEGSSVTLTC-SCDANPADKFRWYKNNQPLLLEDP----------RLILRSVQRSDSGKFHCVAENE---LGEAASDYVFINVEYPP---ETSSVSVSPSAEVLEGSSVTLTCSSDANPAAKYTWFKDNRPLLS-------EDKVHFSSIRSEHSGNYS----------------------CKSENKHG--QSSSTPLL----LDVQYPPRIPSVSVFGKILKGSSVTLTCSSDANP-AANFSWYKKDEDSPKASGTNWTISDFRAEHSGFYYCEAENRRGSLNSSLQL 384
BLAST of NPHS1-3 vs. Ensembl Medaka
Match: kirrel3l (kirre like nephrin family adhesion molecule 2 [Source:NCBI gene;Acc:101173407]) HSP 1 Score: 64.6994 bits (156), Expect = 5.015e-10 Identity = 35/100 (35.00%), Postives = 48/100 (48.00%), Query Frame = 3
Query: 177 DYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYI 476
D V+ S+ + CV+ G VQW L LG +R +PG R ++GD GEH+L I A+ D YECQ L ++ L V PS P +
Sbjct: 26 DQVVVSGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLMGDPLSGEHSLLIDSAELADDAVYECQATQAG---LRSHRAKLTVLVPPSDPVV 122
BLAST of NPHS1-3 vs. Planmine SMEST
Match: SMESG000070688.1 (SMESG000070688.1) HSP 1 Score: 2426.74 bits (6288), Expect = 0.000e+0 Identity = 1245/1252 (99.44%), Postives = 1250/1252 (99.84%), Query Frame = 3
Query: 36 MYQIWIVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVXXXXXXXXXXXXXXXXXXVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSEDIQMKLDPSSEPKELIIDQETIKF 3791
MYQIWIVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYKNEIPKNNEIIIDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNKSSLSLPLSLLKSGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQK+FCEAKSENVKVIPVKSNFLIINVIFPP HLEISC+NCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSKLNLLLNYKQNNFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEP DRIAYRDS+DFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLH+YVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVNLENLNLSIYYIIIASSIVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSE+IQMKLDPSSEPKELIIDQETIKF
Sbjct: 1 MYQIWIVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYKNEIPKNNEIIIDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNKSSLSLPLSLLKSGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKLFCEAKSENVKVIPVKSNFLIINVIFPPAHLEISCINCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSKLNLLLNYKQNNFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPTDRIAYRDSKDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHIYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVNLENLNLSIYYIIIASSIVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSENIQMKLDPSSEPKELIIDQETIKF 1252 HSP 2 Score: 85.5001 bits (210), Expect = 2.105e-16 Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 1
Query: 3829 PVLCQGLYGRFIMEKWQPCDCHLSMERDGFVLMT 3930
PVLCQGLYGRFIMEKWQPCDCHLSMERDGFVLMT
Sbjct: 1299 PVLCQGLYGRFIMEKWQPCDCHLSMERDGFVLMT 1332
BLAST of NPHS1-3 vs. Planmine SMEST
Match: SMESG000070688.1 (SMESG000070688.1) HSP 1 Score: 2395.16 bits (6206), Expect = 0.000e+0 Identity = 1229/1236 (99.43%), Postives = 1234/1236 (99.84%), Query Frame = 3
Query: 84 DSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVXXXXXXXXXXXXXXXXXXVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSEDIQMKLDPSSEPKELIIDQETIKF 3791
DSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYKNEIPKNNEIIIDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNKSSLSLPLSLLKSGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQK+FCEAKSENVKVIPVKSNFLIINVIFPP HLEISC+NCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSKLNLLLNYKQNNFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEP DRIAYRDS+DFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLH+YVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVNLENLNLSIYYIIIASSIVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSE+IQMKLDPSSEPKELIIDQETIKF
Sbjct: 66 DSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYKNEIPKNNEIIIDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNKSSLSLPLSLLKSGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKLFCEAKSENVKVIPVKSNFLIINVIFPPAHLEISCINCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSKLNLLLNYKQNNFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPTDRIAYRDSKDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHIYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKNDTFDGTNTKSQTHVEYPDVNLENLNLSIYYIIIASSIVVFLSLFFALFIFVCIMKHRRKSSVSLQNRKKSLAVDSLLKLPDTLNESKLMADQLDNSENIQMKLDPSSEPKELIIDQETIKF 1301 HSP 2 Score: 85.5001 bits (210), Expect = 2.133e-16 Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 1
Query: 3829 PVLCQGLYGRFIMEKWQPCDCHLSMERDGFVLMT 3930
PVLCQGLYGRFIMEKWQPCDCHLSMERDGFVLMT
Sbjct: 1348 PVLCQGLYGRFIMEKWQPCDCHLSMERDGFVLMT 1381
BLAST of NPHS1-3 vs. Planmine SMEST
Match: SMESG000070688.1 (SMESG000070688.1) HSP 1 Score: 2127.44 bits (5511), Expect = 0.000e+0 Identity = 1073/1083 (99.08%), Postives = 1079/1083 (99.63%), Query Frame = 3
Query: 84 DSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNFSI 3332
DSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYKNEIPKNNEIIIDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNKSSLSLPLSLLKSGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQK+FCEAKSENVKVIPVKSNFLIINVIFPP HLEISC+NCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSKLNLLLNYKQNNFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEP DRIAYRDS+DFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLH+YVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITIS+ +
Sbjct: 66 DSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPVDAHPLLRRKSTLHVTKLPSIPYILYKNEIPKNNEIIIDFNVSVKKMLKLICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNKSSLSLPLSLLKSGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQIVDENNDMEFTCSMKPTGHPKSKLKWSMNITAGFRMENLIKIKYNENQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHLDIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKLDHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKLFCEAKSENVKVIPVKSNFLIINVIFPPAHLEISCINCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSIEIGNSTNCSSTVMQGTLSTNFPGQYNGWKTQSKLNLLLNYKQNNFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLNSLYVFEGSTVNLQYQISANPKATHFSWYFQNRFISSKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSINIFNDSAEFFCEASMNPIYSNFSVVWRRIEPTDRIAYRDSKDFLPILSINYSCNSNSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEIIFSTNTTEIRQSTFCGFLHIYVKEESYFGQYKCQAKNERGSSRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISSIKL 1148
BLAST of NPHS1-3 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 411.379 bits (1056), Expect = 2.353e-120 Identity = 330/1191 (27.71%), Postives = 568/1191 (47.69%), Query Frame = 3
Query: 24 IEKKMYQIWIVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPV-DAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLK--LICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQ------IVDENNDMEFTCSMKPTGHPKSKLKWSMNITA-----GFRMENL----IKIKYNE---NQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHL--DIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKL---DHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSI--EIGN---STNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLN-SLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS---------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSI---NIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNS----NSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII-----FSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGS-SRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNF-SIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKND 3431
++K + + + ++ S++ SS + QS +C+ E+ N IQCF+EIP Y V + + C+V NQ+GKVQWRA ++LGYDR++PG P +++G V KGEH L+I+ +D+G YECQVQPV HPLLRRK+ L V P P I YK P +++II+ S+K+ ++ CVS+ G P+ FQWF N+ + +E L+ + ++S LSL S K G I C + N+ATE+ + + + +V +++ G P I + N +VD+N M TC P+G P L W R EN I +Y + +++++++ L + L H + C++ +P I+ S ++Y+ H P K+ I ++ S I ++ L ++I G E+ + C T YY +KW+ F + +SP+ K+ D +N TI KIK+ + + F++C A + + ++ + K ++ Y + I GY PV +TC + GGNP P L+W++++ + S + ++TL + +DN K FC+AKS ++K P+ S+ + + V+FPP+ + + L+ ++ + + C + SNP +++ WKL C + E+G T C+ ++ + S G++ G QS + ++K + + C+ + + E+++ + K I++ V + P +N + Y+ EG + L + ANP ++W+ + +S SK LII+N D +Y VV N G + + +++ Y P ++ I +AE C NP + S W ++ P S D +P +I CN + + + S C + ++ S LII +D G Y+C+ NGI + + L P L + ++SK ++ L C SH +P + W N + + N T R + L + + + G Y C+A+N +GS SR VS T PD P ++ ++ I +SWI+G DGGS QTFQ+RW ++ S +RY+ V + +K TL I+ S +Y I I + ++ ++ I +S N+
Sbjct: 1 MQKSHFLLVLCLIFDSEM---SSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIIN---SIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENS-ALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRS---LSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIM--TCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISV--------SDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWV-----FSPNPAINNDFISPVEKIWKEDESFRRNSTI------KIKINKQFDQSFLDCQAWSGIK-SVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGA--EVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCE--VFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTI--GCNQAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTST--PDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNE 1151
BLAST of NPHS1-3 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 411.379 bits (1056), Expect = 3.474e-120 Identity = 328/1170 (28.03%), Postives = 557/1170 (47.61%), Query Frame = 3
Query: 87 SSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYVLINDSIAISCVVNNQHGKVQWRAKNLVLGYDRSVPGMPRLQIIGDVTKGEHNLKITKAQTEDSGDYECQVQPV-DAHPLLRRKSTLHVTKLPSIPYILYXXXXXXXXXXXXDFNVSVKKMLK--LICVSEGGLPQPIFQWFWNSREVTTDEMEFILIINIQPNXXXXXXXXXXXXXGHQILCVIKNKATELLESRLQSTYQRFEAKVTVIMFVRSGNPLIQNSNQ------IVDENNDMEFTCSMKPTGHPKSKLKWSMNITA-----GFRMENL----IKIKYNE---NQDELITNLLLKKLNRTFHRVNVSCISESPFITKSSPPFQIYVKHNPQKLSIVTFNEESDNPNEKSSFPPFIQQNHL--DIISGIENEDINVLCFTSSYYGTLNMKWILQHGDFVDGSKLTIQILSPLLKL---DHYLSKNHTIQTFITQKIKLKRTLNSGFIECHATTENQILLKQKTKFNVFYQAKISVISGYVSQFPVHEGDIIHLTCCSYGGNPSPTLRWYKNNSLVLFSDEIKTDKEEQTLDFLYRVFKHDNGQKVFCEAKSENVKVIPVKSNFLIINVIFPPVHLEISCLNCIPNKIMANRTNEFLCESSSSNPGSIITWKLLNCSSI--EIGN---STNCSSTVMQGTLSTNFPGQYNGWKTQSXXXXXXXXXXXXFILQCQSRLSHKILPEKYKPIVKNIKLKVLYPPFIMNSLN-SLYVFEGSTVNLQYQISANPKATHFSWYFQNRFIS---------SKQNLIIKNIHFNDSGSYKFVVENLLGSATRSFEIDVKYSPKVVKTSI---NIFNDSAEFFCEASMNPIYSNFSVVWRRIEPNDRIAYRDSRDFLPILSINYSCNS----NSKPTNQMRINSNCGINKNNKIYSKLIISPYQESDFGWYQCMFCNGIGMKGRENLHLNIPIKPWYLKISQYSKQGTDISHHSLLTCCFLSHVNPSIFWLVPFSNEII-----FSTNTTEIRQSTFCGFLHMYVKEESYFGQYKCQAKNERGS-SRLAYVSLLPTFQPDFPESFKLFILFKNNIHLSWIKGADGGSEQTFQLRWWDEKQQSTFRYQTVKGNQSKETLEITISNF-SIGSYSIDIGVNSIRYPDFRYCCAISFVVLSDKND 3431
SS + QS +C+ E+ N IQCF+EIP Y V + + C+V NQ+GKVQWRA ++LGYDR++PG P +++G V KGEH L+I+ +D+G YECQVQPV HPLLRRK+ L V P P I YK P +++II+ S+K+ ++ CVS+ G P+ FQWF N+ + +E L+ + ++S LSL S K G I C + N+ATE+ + + + +V +++ G P I + N +VD+N M TC P+G P L W R EN I +Y + +++++++ L + L H + C++ +P I+ S ++Y+ H P K+ I ++ S I ++ L ++I G E+ + C T YY +KW+ F + +SP+ K+ D +N TI KIK+ + + F++C A + + ++ + K ++ Y + I GY PV +TC + GGNP P L+W++++ + S + ++TL + +DN K FC+AKS ++K P+ S+ + + V+FPP+ + + L+ ++ + + C + SNP +++ WKL C + E+G T C+ ++ + S G++ G QS + ++K + + C+ + + E+++ + K I++ V + P +N + Y+ EG + L + ANP ++W+ + +S SK LII+N D +Y VV N G + + +++ Y P ++ I +AE C NP + S W ++ P S D +P +I CN + + + S C + ++ S LII +D G Y+C+ NGI + + L P L + ++SK ++ L C SH +P + W N + + N T R + L + + + G Y C+A+N +GS SR VS T PD P ++ ++ I +SWI+G DGGS QTFQ+RW ++ S +RY+ V + +K TL I+ S +Y I I + ++ ++ I +S N+
Sbjct: 38 SSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIIN---SIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENS-ALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRS---LSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIM--TCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISV--------SDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWV-----FSPNPAINNDFISPVEKIWKEDESFRRNSTI------KIKINKQFDQSFLDCQAWSGIK-SVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGA--EVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCE--VFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTI--GCNQAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTST--PDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSADNE 1170 The following BLAST results are available for this feature:
BLAST of NPHS1-3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of NPHS1-3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of NPHS1-3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of NPHS1-3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of NPHS1-3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of NPHS1-3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of NPHS1-3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of NPHS1-3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of NPHS1-3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of NPHS1-3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of NPHS1-3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of NPHS1-3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of NPHS1-3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of NPHS1-3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010202 ID=SMED30010202|Name=NPHS1-3|organism=Schmidtea mediterranea sexual|type=transcript|length=3930bpback to top protein sequence of SMED30010202-orf-1 >SMED30010202-orf-1 ID=SMED30010202-orf-1|Name=SMED30010202-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1253bp MYQIWIVIVLGSKIIYDSSPLPVTQSQKCSARELKNYIQCFKEIPLTDYYback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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