SMED30010198
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010198 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30010198 aligns in the following genomic locations:
Homology
BLAST of SMED30010198 vs. TrEMBL
Match: A0A0X9SRF7 (Putative BRCA2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 68.1662 bits (165), Expect = 1.249e-11 Identity = 34/81 (41.98%), Postives = 52/81 (64.20%), Query Frame = -2 Query: 1431 MLDFNVRKFDSRSTTEAEKRIKDITELLQIESQSLISAFYIL*KD*GAVLWKCYRDSCSTDEKAKNWFVDCFTIKKQLQDR 1673 +L+FN+RKF+ R+ EAE+ +KDI + L I SLI F +L + + WK ++ TDE A++WFVD FT +K + D+ Sbjct: 347 ILNFNIRKFNGRNIFEAEQLLKDIDDWLGIRDLSLIGIFDLLLSEEAKIPWKDFKTIGLTDEDARSWFVDTFTSQKSIADK 427 HSP 2 Score: 31.9574 bits (71), Expect = 1.249e-11 Identity = 16/29 (55.17%), Postives = 22/29 (75.86%), Query Frame = -1 Query: 1363 KTITRQIFDLNAVKQKANDRLATFEIRVR 1449 K+I +I+DL VKQK N+ ATFE+RV+ Sbjct: 422 KSIADKIYDLAHVKQK-NEGFATFELRVK 449
BLAST of SMED30010198 vs. Planmine SMEST
Match: SMESG000078154.1 (SMESG000078154.1) HSP 1 Score: 184.496 bits (467), Expect = 2.536e-77 Identity = 92/104 (88.46%), Postives = 97/104 (93.27%), Query Frame = -3 Query: 881 TIFESRNTNEIQVNSYANVIRRENKNNIQEDVKSKNYTNRHQQNKFLIRPQYVQDDNKYISIANENYNKNIPRKQPSVSLKSIARKLYDESRGIKPSQPEILNP 1192 +IFESRNTN+IQVNSYANVIRRENKNN QEDVKSKNYTNR+QQN FL PQYVQDDNKY +IANENYNKN+PRKQPSVSLKSIARKLYDESRG KP QPEILNP Sbjct: 126 SIFESRNTNKIQVNSYANVIRRENKNNNQEDVKSKNYTNRYQQNNFLKCPQYVQDDNKYTAIANENYNKNMPRKQPSVSLKSIARKLYDESRGTKPHQPEILNP 229 HSP 2 Score: 125.176 bits (313), Expect = 2.536e-77 Identity = 62/73 (84.93%), Postives = 68/73 (93.15%), Query Frame = -1 Query: 1213 IFDLNAVKQKANDRLATFEIRVRNMVKTIFNSGMSAEDIVQDIIANKVWDDRVKEILLKKSSIQMEEVRSTAK 1431 IFDLN+VKQ+AN+R TFEIRVRN+VKT+FNSGMS EDIV+DIIANKV DDRVKEILL KSSIQMEEVRSTAK Sbjct: 46 IFDLNSVKQEANERFTTFEIRVRNLVKTVFNSGMSEEDIVRDIIANKVRDDRVKEILLTKSSIQMEEVRSTAK 118
BLAST of SMED30010198 vs. Planmine SMEST
Match: SMESG000017400.1 (SMESG000017400.1) HSP 1 Score: 240.736 bits (613), Expect = 1.556e-76 Identity = 130/180 (72.22%), Postives = 153/180 (85.00%), Query Frame = -3 Query: 662 IRHTIFESRNTNEIQVNSYANVIRRENKNNIQEDVKSKNYTNRHQQNKFLIRPQYVQDDNKYISIANENYNKNIPRKQPSVSLKSIARKLYDESRGIKPSQPEILNPGNCFCCGENGNRRYQCPMKNKCLICDKENHTFGKCHLMGNHRSQMRRTVACIYXXXXXXXXXXXXNSFASKDQ 1201 I +I E RNTNEIQVNSY+N +RRENK++ QED+K KNY NR+QQ+ FL R QYVQD+NKY +IAN+NY KN+PRKQPSVSLKSI RK+YD SR IK QPEILNPGNCF C E+ RRYQ PMKNKCLIC KENHTFG+CHL+G+HR Q RRTVACI+EEDNND+NF+DNNS+AS ++ Sbjct: 45 ITLSISEIRNTNEIQVNSYSNAVRRENKSDNQEDIKLKNYINRYQQSNFLKRSQYVQDNNKYTAIANKNYYKNMPRKQPSVSLKSITRKIYDGSRVIKQRQPEILNPGNCFYCEESALRRYQYPMKNKCLICGKENHTFGRCHLLGSHRPQRRRTVACIHEEDNNDKNFDDNNSYASGER 224 HSP 2 Score: 65.855 bits (159), Expect = 1.556e-76 Identity = 33/40 (82.50%), Postives = 36/40 (90.00%), Query Frame = -1 Query: 1213 MSAEDIVQDIIANKVWDDRVKEILLKKSSIQMEEVRSTAK 1332 MS EDIV+DII+NKV +DRVKEILL KS IQMEEVRSTAK Sbjct: 1 MSEEDIVRDIISNKVREDRVKEILLTKSRIQMEEVRSTAK 40
BLAST of SMED30010198 vs. Planmine SMEST
Match: SMESG000029320.1 (SMESG000029320.1) HSP 1 Score: 111.694 bits (278), Expect = 1.192e-61 Identity = 60/121 (49.59%), Postives = 81/121 (66.94%), Query Frame = -1 Query: 256 CFYFVSGIEQLNLERKRNDIKRDVMYKTIDKRKWSLMTVNGVDVEMLWDSGAVMSRSMCEKIGNPSVGESGITLRGVFSSGNEKPLGSTIVSVLWNGKVRKISIIIVEKIKPEFIGSVETI 618 C +FVS +E ++ +KRN K+DV++K D RKWS+M +NG VEMLWDSGA + R M EK + +S L GVFS +EKPLGS + WN K R++++IIV KI P+FIG V T+ Sbjct: 242 CLHFVSRVETVDEFKKRNVEKKDVIFKKDDDRKWSMMEINGFHVEMLWDSGASI-RVMSEKCWR--LIDSRERLSGVFSKEDEKPLGSVKIFAKWNKKFRELNVIIVRKIHPDFIGGVYTM 359 HSP 2 Score: 90.8929 bits (224), Expect = 1.192e-61 Identity = 46/96 (47.92%), Postives = 67/96 (69.79%), Query Frame = -2 Query: 3 GVW--KQFGIKILEINNIEATLVNQNYSDLVGIKKAMSFLNKEKNPK----IERFGNIFMVSKFDLGFTDLVSHEMKING*PIMQAPRRQPMHM*G 272 GV+ K FG+++ E+NNIE++LVN+ +D +K + L +KN K I +F NIFM S+FDLG T +++HE+K +G PI+Q PRRQPMH+ G Sbjct: 355 GVYTMKIFGMELKEVNNIESSLVNKRVTDSDRLKNTLLTLKFDKNSKLGTLISQFSNIFMASRFDLGHTKVITHEIKTSGPPILQNPRRQPMHLEG 450 HSP 3 Score: 75.8702 bits (185), Expect = 1.192e-61 Identity = 50/157 (31.85%), Postives = 85/157 (54.14%), Query Frame = -3 Query: 722 VNSYANVIRRENKN--NIQEDVKSKNYTNRHQQNKFLIRPQYV---QDDNKYISIANENYNKNI-PRKQPSVSLK---SIARKLYDESRGIKPSQPEILNPGNCFCCGENGNRRYQCPMKNKCLICDKENHTFGKCHLMGNHRS---QMRRTVACIY 1156 +++ AN+ R E +N + E + + R ++ + +I+ YV QD K +++N I P Q ++ + + +D+ KP + E L PG CFCCG+N + R++C +K+KCLIC KE H+F C+L+ RS ++ R V CI+ Sbjct: 60 ISTKANITREEIRNLAKVYEAREGYDTKKRSEEVQAVIKSTYVNVTQDKQK--QWIQKSFNNRIYPNLQTEITYQNRNERNERQFDKVTTFKPVE-ERLKPGQCFCCGQNDHLRFECTLKDKCLICGKEGHSFRSCNLLVKSRSNTQRLERRVYCIH 213 HSP 4 Score: 65.0846 bits (157), Expect = 1.016e-10 Identity = 30/74 (40.54%), Postives = 55/74 (74.32%), Query Frame = -1 Query: 1213 QIFDLNAVKQKANDRLATFEIRVRNMVKTIFNSGMSAEDIVQDIIANKVWDDRVKEILLKKSSIQMEEVRSTAK 1434 +I +L++V+Q ++R TFEIRV+ +V +++ G+S E+IV+ I+ ++V +DR+KE + K++I EE+R+ AK Sbjct: 3 KICELSSVRQNPDERFVTFEIRVKKLVDEVYDCGISKENIVKLILCHRVQNDRLKENISTKANITREEIRNLAK 76
BLAST of SMED30010198 vs. Planmine SMEST
Match: SMESG000029278.1 (SMESG000029278.1) HSP 1 Score: 95.5153 bits (236), Expect = 8.930e-60 Identity = 52/114 (45.61%), Postives = 71/114 (62.28%), Query Frame = -1 Query: 274 VSGIEQLNLERKRNDIKRDVMYKTIDKRKWSLMTVNGVDVEMLWDSG---AVMSRSMCEKIGNPSVGESGITLRGVFSSGNEKPLGSTIVSVLWNGKVRKISIIIVEKIKPEFI 606 V ++LN+E+K DVM+K D RKW++M +NG V+MLWDSG +VMS IG+P + ES I L GVFS ++KPLGS + WN R+++I V KI P+FI Sbjct: 345 VDEFKKLNVEKK------DVMFKKDDDRKWTMMEINGYHVKMLWDSGTSISVMSEKCWRLIGSPILIESRIRLSGVFSKEDKKPLGSVKIVAKWNKTFRELNITTVRKIHPDFI 452 HSP 2 Score: 89.3521 bits (220), Expect = 8.930e-60 Identity = 52/150 (34.67%), Postives = 82/150 (54.67%), Query Frame = -3 Query: 722 NSYANVIRRENKNNIQEDVKSKNYTNRHQQNKFLIRPQYVQDDNKYISIANENYNKNIP---RKQPSVSLKSIARKLYDESRGIKPSQPEILNPGNCFCCGENGNRRYQCPMKNKCLICDKENHTFGKCHLMGNHRS---QMRRTVACIY 1153 ++Y NV + K IQ+ ++ Y NR + + R + + + + YN+ R P VS+K IARK+Y+ G+ E L PG CFCCG+N + +++ P+K+KCLIC KE H+F C+L+ RS ++ R V CI+ Sbjct: 162 STYVNVALDKQKQWIQKSFNNRIYPNRQTEITYQNRNERNERQFDKVKPVAKQYNEKTTTERRPPPLVSMKHIARKIYNRCCGLPEPVEERLKPGQCFCCGQNDHMQFEYPLKDKCLICGKEGHSFRSCNLLVKSRSNTQRLERRVYCIH 311 HSP 3 Score: 73.1738 bits (178), Expect = 8.930e-60 Identity = 37/91 (40.66%), Postives = 58/91 (63.74%), Query Frame = -2 Query: 1431 LESLSKQAHSMLDFNVRKFDSRSTTEAEKRIKDITELLQIESQSLISAFYIL*KD*GAVLWKCYRDSCSTDEKAKNWFVDCFTIKKQLQDR 1703 ++ L KQA +L+FNVR+F R TEAE+ +KDI + + + +L+S F +L + + LWK ++D +T E A+ WF D FT KK + D+ Sbjct: 1 MKCLPKQAKDILNFNVRRFYGRCMTEAEQWLKDIEDWMLLNDLNLVSIFDLLFSEEASFLWKKFKDISTTSENAREWFNDTFTKKKSIMDK 91 HSP 4 Score: 33.8834 bits (76), Expect = 8.930e-60 Identity = 19/59 (32.20%), Postives = 32/59 (54.24%), Query Frame = -1 Query: 1276 KTITRQIFDLNAVKQKANDRLATFEIRVRNMVKTIFNSGMSAEDIVQDIIAN-KVWDDR 1449 K+I +I L++V+Q N+R TF+IRV+ +V + + D I KV++ R Sbjct: 86 KSIMDKICKLSSVRQNHNERFVTFKIRVKKLVDEAYEWNFKRKHRQTDFIPQTKVYEAR 144
BLAST of SMED30010198 vs. Planmine SMEST
Match: SMESG000072638.1 (SMESG000072638.1) HSP 1 Score: 192.586 bits (488), Expect = 8.096e-56 Identity = 90/117 (76.92%), Postives = 98/117 (83.76%), Query Frame = -3 Query: 722 HQQNKFLIRPQYVQDDNKYISIANENYNKNIPRKQPSVSLKSIARKLYDESRGIKPSQPEILNPGNCFCCGENGNRRYQCPMKNKCLICDKENHTFGKCHLMGNHRSQMRRTVACIY 1072 +QQ+ L R Q VQDDNKY +IANENYN+N+PRKQPSVSLK IARKLYDES GI P Q EI N GNC CCGENG+RRYQC M NKCLIC KE HTFG+CHL+GNHR QMRRTVACIY Sbjct: 2 YQQSNVLKRSQSVQDDNKYTAIANENYNRNMPRKQPSVSLKIIARKLYDESLGINPRQLEIPNSGNCICCGENGHRRYQCSMTNKCLICRKEKHTFGRCHLLGNHRPQMRRTVACIY 118 HSP 2 Score: 45.0542 bits (105), Expect = 8.096e-56 Identity = 19/29 (65.52%), Postives = 22/29 (75.86%), Query Frame = -1 Query: 565 KKTWKNHGESHCFYFVSGIEQLNLERKRN 651 +K K HG SHCF+ VSGIEQLN ER+ N Sbjct: 144 RKHGKKHGGSHCFHLVSGIEQLNFERRLN 172 The following BLAST results are available for this feature:
BLAST of SMED30010198 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30010198 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30010198 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of SMED30010198 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30010198 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30010198 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010198 ID=SMED30010198|Name=SMED30010198|organism=Schmidtea mediterranea sexual|type=transcript|length=1704bpback to top protein sequence of SMED30010198-orf-1 >SMED30010198-orf-1 ID=SMED30010198-orf-1|Name=SMED30010198-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=106bp MYKTIDKRKWSLMTVNGVDVEMLWDSGAVMSRSMCEKIGNPSVGESGITLback to top protein sequence of SMED30010198-orf-2 >SMED30010198-orf-2 ID=SMED30010198-orf-2|Name=SMED30010198-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=205bp MKIRHTIFESRNTNEIQVNSYANVIRRENKNNIQEDVKSKNYTNRHQQNKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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