Transcriptional repressor p66-beta

Overview
NameTranscriptional repressor p66-beta
Smed IDSMED30010167
Length (bp)1869
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transcriptional repressor p66-beta (SMED30010167) t-SNE clustered cells

Violin plots show distribution of expression levels for Transcriptional repressor p66-beta (SMED30010167) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transcriptional repressor p66-beta (SMED30010167) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transcriptional repressor p66-beta (SMED30010167) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30010167

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30010167h1SMcG0016798 SmedASXL_011816SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30010167h1SMcG0016798 dd_Smed_v4_3115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010167h1SMcG0016798 dd_Smed_v4_3115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30010167h1SMcG0016798 dd_Smed_v4_3115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010167h1SMcG0016798 dd_Smed_v4_3115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010167h1SMcG0016798 dd_Smed_v4_3115_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010167h1SMcG0016798 EC615093smed_ncbi_20240424PMID:27606067
Vásquez-Doorman et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30010167h1SMcG0016798 EC615093smed_ncbi_20240424PMID:27606067
Vásquez-Doorman et al., 2016
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30010167h1SMcG0016798 dd_Smed_v4_3115_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Transcriptional repressor p66-beta vs. Ensembl Human
Match: GATAD2B (GATA zinc finger domain containing 2B [Source:HGNC Symbol;Acc:HGNC:30778])

HSP 1 Score: 88.1965 bits (217), Expect = 9.405e-19
Identity = 64/197 (32.49%), Postives = 103/197 (52.28%), Query Frame = 3
Query:  498 NNQTDAQAVPGNRTSAHGASNNTTA---PYTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            N Q  + +VP  RT++     N  +   P T   N+ SS  P  +   AM        A K  LRKQ +K L E+P             L+ +PS A +EF+ +VGLEEVV+++ + +    G +   +  V  PF C QC TDFTP WK+E+ G  K++CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:   50 NYQPSSSSVPCQRTTSSAIYMNLASHIQPGT--VNRVSSPLPSPS---AMTDAANSQAAAKLALRKQLEKTLLEIP-----PPKPPAPLLHFLPSAANSEFIYMVGLEEVVQSVIDSQ----GKSCASLLRV-EPFVCAQCRTDFTPHWKQEKNG--KILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIEQ 229          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Human
Match: GATAD2A (GATA zinc finger domain containing 2A [Source:HGNC Symbol;Acc:HGNC:29989])

HSP 1 Score: 91.6633 bits (226), Expect = 1.044e-18
Identity = 49/138 (35.51%), Postives = 78/138 (56.52%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLD 1076
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +    G  +    +   P+ C QC TDFT  W+ E+ G+  ++CE+C   N ++AL+ ++  +++    K   QE++++
Sbjct:  347 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQ----GRMSAATVLSREPYMCAQCKTDFTCRWREEKSGA--IMCENCMTTNQKKALKVEHTSRLKAAFVKALQQEQEIE 473          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Human
Match: GATAD2A (GATA zinc finger domain containing 2A [Source:HGNC Symbol;Acc:HGNC:29989])

HSP 1 Score: 91.6633 bits (226), Expect = 1.044e-18
Identity = 49/138 (35.51%), Postives = 78/138 (56.52%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLD 1076
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +    G  +    +   P+ C QC TDFT  W+ E+ G+  ++CE+C   N ++AL+ ++  +++    K   QE++++
Sbjct:  347 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQ----GRMSAATVLSREPYMCAQCKTDFTCRWREEKSGA--IMCENCMTTNQKKALKVEHTSRLKAAFVKALQQEQEIE 473          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Human
Match: GATAD2A (GATA zinc finger domain containing 2A [Source:HGNC Symbol;Acc:HGNC:29989])

HSP 1 Score: 91.6633 bits (226), Expect = 1.093e-18
Identity = 49/138 (35.51%), Postives = 78/138 (56.52%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLD 1076
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +    G  +    +   P+ C QC TDFT  W+ E+ G+  ++CE+C   N ++AL+ ++  +++    K   QE++++
Sbjct:  347 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQA---GRMSAATVLSREPYMCAQCKTDFTCRWREEKSGA--IMCENCMTTNQKKALKVEHTSRLKAAFVKALQQEQEIE 474          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Human
Match: GATAD2B (GATA zinc finger domain containing 2B [Source:HGNC Symbol;Acc:HGNC:30778])

HSP 1 Score: 85.8853 bits (211), Expect = 7.937e-17
Identity = 49/138 (35.51%), Postives = 80/138 (57.97%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLD 1076
            A K  LRKQ +K L E+P             L+ +PS A +EF+ +VGLEEVV+++ + +    G +   +  V  PF C QC TDFTP WK+E+ G  K++CE C   N ++AL+ ++ ++++    K   QE++++
Sbjct:  351 AAKLALRKQLEKTLLEIPPP-----KPPAPLLHFLPSAANSEFIYMVGLEEVVQSVIDSQ----GKSCASLLRV-EPFVCAQCRTDFTPHWKQEKNG--KILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIE 476          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Celegans
Match: dcp-66 (Deacetylase Complex Protein [Source:UniProtKB/TrEMBL;Acc:G5ED89])

HSP 1 Score: 50.447 bits (119), Expect = 3.842e-6
Identity = 37/148 (25.00%), Postives = 68/148 (45.95%), Query Frame = 3
Query:  651 QQYLARKALLRKQADKALSEVPLARP-PQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSK----VVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            Q+  A +   R QADK L+ +P  +  P       D+  +P+   + F+AL GL+ VV+++       L   +NE       + C +C T+    WK    GS++    + CE+C     +R  + D    ++   +K   QE++ ++
Sbjct:  372 QRIQAARLAFRAQADKQLTTIPTQKSQPH------DITFLPNPNASAFLALHGLDLVVQHV-------LKDRSNETPYAGPSYECEECKTECAHTWKA--IGSTQDDLHLYCENCVRSAQKRKNRTDQTALLKRAFQKITAQEKEFEK 504          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Fly
Match: simj (gene:FBgn0010762 transcript:FBtr0331573)

HSP 1 Score: 82.8037 bits (203), Expect = 4.235e-16
Identity = 48/157 (30.57%), Postives = 79/157 (50.32%), Query Frame = 3
Query:  615 RMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSS--KVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            R T     Q   Q+  A K  LRKQ +K L ++P            +++ IP+ +  +FV L+GLE VV  L   +         +   V  PF C QC  DFTP+WK E+  +   KV+CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  421 RSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPP-----KPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNK---------KANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQ 563          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Fly
Match: simj (gene:FBgn0010762 transcript:FBtr0076264)

HSP 1 Score: 82.8037 bits (203), Expect = 4.352e-16
Identity = 48/157 (30.57%), Postives = 79/157 (50.32%), Query Frame = 3
Query:  615 RMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSS--KVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            R T     Q   Q+  A K  LRKQ +K L ++P            +++ IP+ +  +FV L+GLE VV  L   +         +   V  PF C QC  DFTP+WK E+  +   KV+CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  413 RSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPP-----KPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNK---------KANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQ 555          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Fly
Match: simj (gene:FBgn0010762 transcript:FBtr0076263)

HSP 1 Score: 82.8037 bits (203), Expect = 4.555e-16
Identity = 48/157 (30.57%), Postives = 79/157 (50.32%), Query Frame = 3
Query:  615 RMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSS--KVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            R T     Q   Q+  A K  LRKQ +K L ++P            +++ IP+ +  +FV L+GLE VV  L   +         +   V  PF C QC  DFTP+WK E+  +   KV+CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  413 RSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPP-----KPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNK---------KANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQ 555          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Fly
Match: simj (gene:FBgn0010762 transcript:FBtr0076262)

HSP 1 Score: 82.8037 bits (203), Expect = 4.555e-16
Identity = 48/157 (30.57%), Postives = 79/157 (50.32%), Query Frame = 3
Query:  615 RMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSS--KVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            R T     Q   Q+  A K  LRKQ +K L ++P            +++ IP+ +  +FV L+GLE VV  L   +         +   V  PF C QC  DFTP+WK E+  +   KV+CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  413 RSTPREDAQTPAQRQAAAKMALRKQLEKTLLQIPPP-----KPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNK---------KANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQ 555          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Fly
Match: simj (gene:FBgn0010762 transcript:FBtr0331574)

HSP 1 Score: 82.8037 bits (203), Expect = 4.734e-16
Identity = 45/145 (31.03%), Postives = 76/145 (52.41%), Query Frame = 3
Query:  651 QQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSS--KVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            Q+  A K  LRKQ +K L ++P            +++ IP+ +  +FV L+GLE VV  L   +         +   V  PF C QC  DFTP+WK E+  +   KV+CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  425 QRQAAAKMALRKQLEKTLLQIPPP-----KPPPPEMHFIPNPSNTDFVYLLGLETVVDYLTVNK---------KANAVAAPFRCAQCKIDFTPVWKWEKQTNKDPKVICEQCVTSNVKKALKAEHTNRLKQAFVKALQQEQEIEQ 555          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Zebrafish
Match: gatad2ab (GATA zinc finger domain containing 2Ab [Source:ZFIN;Acc:ZDB-GENE-040426-2372])

HSP 1 Score: 86.6557 bits (213), Expect = 2.790e-17
Identity = 50/151 (33.11%), Postives = 78/151 (51.66%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVF--TPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPK 1109
            A K  LRKQ +K L E+P            +LN +PS A NEF+ LVGLE VV+NL +    +    ++         PF C QC TDFT  W++E+   S ++CE C   N ++ L+ ++  +++    K   QE+++++       SPK
Sbjct:  323 AAKLALRKQLEKTLLEIPPP-----KPPAPELNFLPSAANNEFIYLVGLEMVVQNLLDTLAKSKHQQSDSASNTAPKEPFTCAQCQTDFTSRWRQEKNNGS-ILCEHCMSSNQKKVLKAEHTSRLKAAFVKALQQEQEIEQRILQQAASPK 467          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Zebrafish
Match: gatad2ab (GATA zinc finger domain containing 2Ab [Source:ZFIN;Acc:ZDB-GENE-040426-2372])

HSP 1 Score: 86.6557 bits (213), Expect = 2.790e-17
Identity = 50/151 (33.11%), Postives = 78/151 (51.66%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVF--TPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPK 1109
            A K  LRKQ +K L E+P            +LN +PS A NEF+ LVGLE VV+NL +    +    ++         PF C QC TDFT  W++E+   S ++CE C   N ++ L+ ++  +++    K   QE+++++       SPK
Sbjct:  323 AAKLALRKQLEKTLLEIPPP-----KPPAPELNFLPSAANNEFIYLVGLEMVVQNLLDTLAKSKHQQSDSASNTAPKEPFTCAQCQTDFTSRWRQEKNNGS-ILCEHCMSSNQKKVLKAEHTSRLKAAFVKALQQEQEIEQRILQQAASPK 467          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Zebrafish
Match: gatad2ab (GATA zinc finger domain containing 2Ab [Source:ZFIN;Acc:ZDB-GENE-040426-2372])

HSP 1 Score: 86.6557 bits (213), Expect = 2.826e-17
Identity = 50/151 (33.11%), Postives = 78/151 (51.66%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVF--TPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPK 1109
            A K  LRKQ +K L E+P            +LN +PS A NEF+ LVGLE VV+NL +    +    ++         PF C QC TDFT  W++E+   S ++CE C   N ++ L+ ++  +++    K   QE+++++       SPK
Sbjct:  330 AAKLALRKQLEKTLLEIPPP-----KPPAPELNFLPSAANNEFIYLVGLEMVVQNLLDTLAKSKHQQSDSASNTAPKEPFTCAQCQTDFTSRWRQEKNNGS-ILCEHCMSSNQKKVLKAEHTSRLKAAFVKALQQEQEIEQRILQQAASPK 474          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Zebrafish
Match: gatad2b (GATA zinc finger domain containing 2B [Source:ZFIN;Acc:ZDB-GENE-060503-655])

HSP 1 Score: 86.6557 bits (213), Expect = 2.842e-17
Identity = 47/138 (34.06%), Postives = 78/138 (56.52%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLD 1076
            A K  LRKQ +K L E+P             L+ +PS A +EF+ +VGLEEVV+++ + +    G   +       PF C QC TDFTP WK+E+ G  +++CE C   N ++AL+ ++ ++++    K   QE++++
Sbjct:  373 AAKLALRKQLEKTLLEIPPP-----KPPAPLLHFLPSAANSEFIYMVGLEEVVQSVIDSQGKMRGSLPH-----VEPFFCAQCRTDFTPHWKQEKSG--RILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIE 498          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Zebrafish
Match: gatad2ab (GATA zinc finger domain containing 2Ab [Source:ZFIN;Acc:ZDB-GENE-040426-2372])

HSP 1 Score: 86.2705 bits (212), Expect = 4.361e-17
Identity = 50/151 (33.11%), Postives = 78/151 (51.66%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVF--TPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPK 1109
            A K  LRKQ +K L E+P            +LN +PS A NEF+ LVGLE VV+NL +    +    ++         PF C QC TDFT  W++E+   S ++CE C   N ++ L+ ++  +++    K   QE+++++       SPK
Sbjct:  365 AAKLALRKQLEKTLLEIPPP-----KPPAPELNFLPSAANNEFIYLVGLEMVVQNLLDTLAKSKHQQSDSASNTAPKEPFTCAQCQTDFTSRWRQEKNNGS-ILCEHCMSSNQKKVLKAEHTSRLKAAFVKALQQEQEIEQRILQQAASPK 509          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Xenopus
Match: rab32 (RAB32, member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-483399])

HSP 1 Score: 90.8929 bits (224), Expect = 1.298e-18
Identity = 70/235 (29.79%), Postives = 114/235 (48.51%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYR-NLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQTPTSTV-----TATQSQGRPSHRPSNTVSTVSGAARTAPTTVSG 1349
            A K  LRKQ +K L E+P            +LN +PS A NEF+ LVGLEEVV+NL E +  ++   +++E      P+ C QC TDFT  W++E+ G+  ++CE C   N ++ L+ ++ ++++    K   QE+++++       SP + +     Q   L    T  +R  + TT+       G  H  P +T      T     G+P  R  N  S ++G  + +P    G
Sbjct:  306 AAKLALRKQLEKTLLEIPPP-----KPPAPELNFLPSAANNEFIYLVGLEEVVQNLLESQGKISQAPSSHE------PYICAQCKTDFTSRWRQEKNGT--IMCEVCMSSNQKKTLKAEHTNRLKAAFVKALQQEQEIEQRILQQSSSPAQSKADHSVQHHSLKQSPTPISRGLSGTTR-------GILHTFPPTTKLPNAGTLANRAGKPGDRSLNK-SNMTGNWKKSPINAGG 519          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Xenopus
Match: rab32 (RAB32, member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-483399])

HSP 1 Score: 90.5077 bits (223), Expect = 2.094e-18
Identity = 70/235 (29.79%), Postives = 114/235 (48.51%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYR-NLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQTPTSTV-----TATQSQGRPSHRPSNTVSTVSGAARTAPTTVSG 1349
            A K  LRKQ +K L E+P            +LN +PS A NEF+ LVGLEEVV+NL E +  ++   +++E      P+ C QC TDFT  W++E+ G+  ++CE C   N ++ L+ ++ ++++    K   QE+++++       SP + +     Q   L    T  +R  + TT+       G  H  P +T      T     G+P  R  N  S ++G  + +P    G
Sbjct:  351 AAKLALRKQLEKTLLEIPPP-----KPPAPELNFLPSAANNEFIYLVGLEEVVQNLLESQGKISQAPSSHE------PYICAQCKTDFTSRWRQEKNGT--IMCEVCMSSNQKKTLKAEHTNRLKAAFVKALQQEQEIEQRILQQSSSPAQSKADHSVQHHSLKQSPTPISRGLSGTTR-------GILHTFPPTTKLPNAGTLANRAGKPGDRSLNK-SNMTGNWKKSPINAGG 564          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Xenopus
Match: GATAD2B (GATA zinc finger domain containing 2B [Source:NCBI gene;Acc:100486823])

HSP 1 Score: 87.8113 bits (216), Expect = 1.434e-17
Identity = 50/139 (35.97%), Postives = 82/139 (58.99%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P             LN +PS A +EF+ +VGLEEVV+++ + +   +   ++ + M   PF C QC TDFTP WKRE+ G  K++CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  337 AAKLALRKQLEKTLLEIPPP-----KPPAPLLNFLPSAANSEFIYMVGLEEVVQSVIDSQ---VKGCSSLLHM--DPFICAQCRTDFTPHWKREKSG--KILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIEQ 463          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Mouse
Match: Gatad2b (GATA zinc finger domain containing 2B [Source:MGI Symbol;Acc:MGI:2443225])

HSP 1 Score: 89.3521 bits (220), Expect = 2.197e-19
Identity = 50/139 (35.97%), Postives = 81/139 (58.27%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P             L+ +PS A +EF+ +VGLEEVV+++ + +    G N   +  V  PF C QC TDFTP WK+E+ G  K++CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  103 AAKLALRKQLEKTLLEIP-----PPKPPAPLLHFLPSAANSEFIYMVGLEEVVQSVIDSQ----GKNCASLLRV-EPFVCAQCRTDFTPHWKQEKNG--KILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIEQ 229          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Mouse
Match: Gatad2a (GATA zinc finger domain containing 2A [Source:MGI Symbol;Acc:MGI:2384585])

HSP 1 Score: 93.2041 bits (230), Expect = 2.309e-19
Identity = 51/139 (36.69%), Postives = 79/139 (56.83%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +   +        +   P+ CVQC TDFT  W RE+ G+  V+CE+C   N ++AL+ ++  +++    K   QE+++++
Sbjct:  345 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQAGRISATAAAAVLSREPYMCVQCKTDFTCRW-REKGGA--VMCENCMTSNQKKALKVEHTSRLKAAFVKALQQEQEMEQ 475          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Mouse
Match: Gatad2a (GATA zinc finger domain containing 2A [Source:MGI Symbol;Acc:MGI:2384585])

HSP 1 Score: 92.8189 bits (229), Expect = 3.719e-19
Identity = 51/139 (36.69%), Postives = 79/139 (56.83%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +   +        +   P+ CVQC TDFT  W RE+ G+  V+CE+C   N ++AL+ ++  +++    K   QE+++++
Sbjct:  343 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQAGRISATAAAAVLSREPYMCVQCKTDFTCRW-REKGGA--VMCENCMTSNQKKALKVEHTSRLKAAFVKALQQEQEMEQ 473          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Mouse
Match: Gatad2a (GATA zinc finger domain containing 2A [Source:MGI Symbol;Acc:MGI:2384585])

HSP 1 Score: 92.8189 bits (229), Expect = 3.777e-19
Identity = 51/139 (36.69%), Postives = 79/139 (56.83%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +   +        +   P+ CVQC TDFT  W RE+ G+  V+CE+C   N ++AL+ ++  +++    K   QE+++++
Sbjct:  342 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQAGRISATAAAAVLSREPYMCVQCKTDFTCRW-REKGGA--VMCENCMTSNQKKALKVEHTSRLKAAFVKALQQEQEMEQ 472          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Mouse
Match: Gatad2a (GATA zinc finger domain containing 2A [Source:MGI Symbol;Acc:MGI:2384585])

HSP 1 Score: 92.8189 bits (229), Expect = 3.777e-19
Identity = 51/139 (36.69%), Postives = 79/139 (56.83%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +   +        +   P+ CVQC TDFT  W RE+ G+  V+CE+C   N ++AL+ ++  +++    K   QE+++++
Sbjct:  342 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQAGRISATAAAAVLSREPYMCVQCKTDFTCRW-REKGGA--VMCENCMTSNQKKALKVEHTSRLKAAFVKALQQEQEMEQ 472          
BLAST of Transcriptional repressor p66-beta vs. UniProt/SwissProt
Match: sp|Q8CHY6|P66A_MOUSE (Transcriptional repressor p66 alpha OS=Mus musculus OX=10090 GN=Gatad2a PE=1 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 2.642e-18
Identity = 51/139 (36.69%), Postives = 79/139 (56.83%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +   +        +   P+ CVQC TDFT  W RE+ G+  V+CE+C   N ++AL+ ++  +++    K   QE+++++
Sbjct:  342 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQAGRISATAAAAVLSREPYMCVQCKTDFTCRW-REKGGA--VMCENCMTSNQKKALKVEHTSRLKAAFVKALQQEQEMEQ 472          
BLAST of Transcriptional repressor p66-beta vs. UniProt/SwissProt
Match: sp|Q86YP4|P66A_HUMAN (Transcriptional repressor p66-alpha OS=Homo sapiens OX=9606 GN=GATAD2A PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.013e-18
Identity = 49/138 (35.51%), Postives = 78/138 (56.52%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLD 1076
            A K  LRKQ +K L E+P            ++N +PS A NEF+ LVGLEEVV+NL E +    G  +    +   P+ C QC TDFT  W+ E+ G+  ++CE+C   N ++AL+ ++  +++    K   QE++++
Sbjct:  347 AAKLALRKQLEKTLLEIPPP-----KPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQ----GRMSAATVLSREPYMCAQCKTDFTCRWREEKSGA--IMCENCMTTNQKKALKVEHTSRLKAAFVKALQQEQEIE 473          
BLAST of Transcriptional repressor p66-beta vs. UniProt/SwissProt
Match: sp|Q8VHR5|P66B_MOUSE (Transcriptional repressor p66-beta OS=Mus musculus OX=10090 GN=Gatad2b PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 8.452e-17
Identity = 50/139 (35.97%), Postives = 81/139 (58.27%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P             L+ +PS A +EF+ +VGLEEVV+++ + +    G N   +  V  PF C QC TDFTP WK+E+ G  K++CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  352 AAKLALRKQLEKTLLEIPPP-----KPPAPLLHFLPSAANSEFIYMVGLEEVVQSVIDSQ----GKNCASLLRV-EPFVCAQCRTDFTPHWKQEKNG--KILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIEQ 478          
BLAST of Transcriptional repressor p66-beta vs. UniProt/SwissProt
Match: sp|Q8WXI9|P66B_HUMAN (Transcriptional repressor p66-beta OS=Homo sapiens OX=9606 GN=GATAD2B PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 3.811e-16
Identity = 49/138 (35.51%), Postives = 80/138 (57.97%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLD 1076
            A K  LRKQ +K L E+P             L+ +PS A +EF+ +VGLEEVV+++ + +    G +   +  V  PF C QC TDFTP WK+E+ G  K++CE C   N ++AL+ ++ ++++    K   QE++++
Sbjct:  351 AAKLALRKQLEKTLLEIPPP-----KPPAPLLHFLPSAANSEFIYMVGLEEVVQSVIDSQ----GKSCASLLRV-EPFVCAQCRTDFTPHWKQEKNG--KILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIE 476          
BLAST of Transcriptional repressor p66-beta vs. TrEMBL
Match: A0A4Z2DRT6 (Transcriptional repressor p66-beta isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_009174 PE=4 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 6.359e-55
Identity = 136/397 (34.26%), Postives = 203/397 (51.13%), Query Frame = 3
Query:  102 AMDLSRKVNS-----------QGSGVICLSEDEDDENINQLLTKLNIEK-PSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKI---PPST--SPANTSTIRQNGHVAQXXXXXXXXXXXXXXXXXXXXXXXXXXETSIENSPNNQTDAQAVP---GNRTSAHGASNNTTAPYTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQ 1232
             +DLSRK N                VI LSEDEDD  +  L  ++  E  P    +   + LL +L+  LRNEE+  +LL Q++  Q+     P ST  S   TST+R        +                               P  Q   + VP   GNR +   A N+T    TN     +++ P++T+ MA+ AL+QQ+  +KA+LRKQ ++ L +V L +P  G    S +  +PST TNEF A++GLEE+V  +Q++  L    ++ + R V  PF+C +CGTDFTP+WKR+RPGSS+VVCE+C  ++ R A+   Y + I   LK++   ER+++ +Y+D+++SP K E F KE E+KLLA                  + TG H+Q
Sbjct:   24 VLDLSRKSNHVDNDDNKLSVRNDDDVIMLSEDEDDRLLGALCDEMEKENTPKQSLLSKSRALLLKLENELRNEESTLVLLQQLRANQRSHALQPRSTKGSSNTTSTVRSTQSPVNQVR------------------------------PAGQIAGRTVPSTTGNRATQSPAQNSTAINSTN-----ATSIPKVTKGMAVHALEQQFSGKKAMLRKQLERTLDKVVLPKPAVGN-GLSVVPFVPSTLTNEFTAMIGLEEIVTTIQDFDPLNTERSSEQSRFV-NPFSCSRCGTDFTPVWKRKRPGSSEVVCEACIIDSQRSAIHKAYNNVISTALKQHAVSEREIEHEYQDVVNSPAKLEAFIKEHERKLLATQQAQMVQQQQQQMQQVASVTGLHNQ 383          
BLAST of Transcriptional repressor p66-beta vs. TrEMBL
Match: A0A504Z1B5 (Transcriptional repressor p66-beta OS=Fasciola gigantica OX=46835 GN=FGIG_04192 PE=4 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 1.761e-54
Identity = 138/382 (36.13%), Postives = 209/382 (54.71%), Query Frame = 3
Query:   36 MEIEVLDLSQKPICLNENDLPTAMDLSRKVNSQGSGVICLSEDEDDENINQLLTKLNIE-KPSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKIPPSTSPANTSTIRQNGHVAQXXXXXXXXXXXXXXXXXXXXXXXXXXETSIENSPN-----NQTDAQAVPGNRTSAHGASNNTTAP---YTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLA 1154
            ME +VLDLS K    +EN   +     +K++     VI LSEDED+  I+ L  ++  E  P+       + LL +++  LRNEE+  +LL Q++  Q+        A+++ +R +    Q  T     +  +N+      ++       I NSP+     +Q+   + P        A +  TAP      + N +S   PR+T+ MA+QAL+QQ+  +KA+L K  +K+L +VPL RP  GA   SD+  +PST TNEF AL+GLEE+V  +Q+Y     G   +E     TPF C  CGTDFTP+WKR+R GSS VVCE+C  +  R  +   Y + +   LK+    ER+L+ +Y+++++SP K E F KEQE+KLLA
Sbjct:  153 MENDVLDLSGK----SENQEKSG---HKKIDDD---VILLSEDEDEAMIDALCNEMEKENAPNSVLHSKTKALLAKMESELRNEESTLLLLQQLRANQR--------AHSTQLRSSKSTTQHSTPKAPVSAHSNSMYYHITASDR-----IANSPSPARMPSQSVVPSRPAQGEFVTDAFHFVTAPSQGLQQQPNVTSHQVPRVTKVMALQALEQQWNGKKAVLHKHLEKSLDKVPLPRPSSGA-GPSDVAFVPSTLTNEFTALIGLEEIVHTIQDYDPSCSG-KADEQHRPCTPFVCANCGTDFTPVWKRKRSGSSDVVCEACIIDGLRSGIHRSYSNMVSAALKQNATSERELEHEYQEIVNSPTKLEAFIKEQERKLLA 509          
BLAST of Transcriptional repressor p66-beta vs. TrEMBL
Match: A0A4Z2DSZ6 (Transcriptional repressor p66-beta isoform 3 OS=Schistosoma japonicum OX=6182 GN=EWB00_009174 PE=4 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 1.111e-53
Identity = 136/397 (34.26%), Postives = 203/397 (51.13%), Query Frame = 3
Query:  102 AMDLSRKVNS-----------QGSGVICLSEDEDDENINQLLTKLNIEK-PSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKI---PPST--SPANTSTIRQNGHVAQXXXXXXXXXXXXXXXXXXXXXXXXXXETSIENSPNNQTDAQAVP---GNRTSAHGASNNTTAPYTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQ 1232
             +DLSRK N                VI LSEDEDD  +  L  ++  E  P    +   + LL +L+  LRNEE+  +LL Q++  Q+     P ST  S   TST+R        +                               P  Q   + VP   GNR +   A N+T    TN     +++ P++T+ MA+ AL+QQ+  +KA+LRKQ ++ L +V L +P  G    S +  +PST TNEF A++GLEE+V  +Q++  L    ++ + R V  PF+C +CGTDFTP+WKR+RPGSS+VVCE+C  ++ R A+   Y + I   LK++   ER+++ +Y+D+++SP K E F KE E+KLLA                  + TG H+Q
Sbjct:  159 VLDLSRKSNHVDNDDNKLSVRNDDDVIMLSEDEDDRLLGALCDEMEKENTPKQSLLSKSRALLLKLENELRNEESTLVLLQQLRANQRSHALQPRSTKGSSNTTSTVRSTQSPVNQVR------------------------------PAGQIAGRTVPSTTGNRATQSPAQNSTAINSTN-----ATSIPKVTKGMAVHALEQQFSGKKAMLRKQLERTLDKVVLPKPAVGN-GLSVVPFVPSTLTNEFTAMIGLEEIVTTIQDFDPLNTERSSEQSRFV-NPFSCSRCGTDFTPVWKRKRPGSSEVVCEACIIDSQRSAIHKAYNNVISTALKQHAVSEREIEHEYQDVVNSPAKLEAFIKEHERKLLATQQAQMVQQQQQQMQQVASVTGLHNQ 518          
BLAST of Transcriptional repressor p66-beta vs. TrEMBL
Match: A0A4Z2DS26 (Transcriptional repressor p66-beta isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_009174 PE=4 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 1.482e-53
Identity = 136/397 (34.26%), Postives = 203/397 (51.13%), Query Frame = 3
Query:  102 AMDLSRKVNS-----------QGSGVICLSEDEDDENINQLLTKLNIEK-PSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKI---PPST--SPANTSTIRQNGHVAQXXXXXXXXXXXXXXXXXXXXXXXXXXETSIENSPNNQTDAQAVP---GNRTSAHGASNNTTAPYTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQ 1232
             +DLSRK N                VI LSEDEDD  +  L  ++  E  P    +   + LL +L+  LRNEE+  +LL Q++  Q+     P ST  S   TST+R        +                               P  Q   + VP   GNR +   A N+T    TN     +++ P++T+ MA+ AL+QQ+  +KA+LRKQ ++ L +V L +P  G    S +  +PST TNEF A++GLEE+V  +Q++  L    ++ + R V  PF+C +CGTDFTP+WKR+RPGSS+VVCE+C  ++ R A+   Y + I   LK++   ER+++ +Y+D+++SP K E F KE E+KLLA                  + TG H+Q
Sbjct:  159 VLDLSRKSNHVDNDDNKLSVRNDDDVIMLSEDEDDRLLGALCDEMEKENTPKQSLLSKSRALLLKLENELRNEESTLVLLQQLRANQRSHALQPRSTKGSSNTTSTVRSTQSPVNQVR------------------------------PAGQIAGRTVPSTTGNRATQSPAQNSTAINSTN-----ATSIPKVTKGMAVHALEQQFSGKKAMLRKQLERTLDKVVLPKPAVGN-GLSVVPFVPSTLTNEFTAMIGLEEIVTTIQDFDPLNTERSSEQSRFV-NPFSCSRCGTDFTPVWKRKRPGSSEVVCEACIIDSQRSAIHKAYNNVISTALKQHAVSEREIEHEYQDVVNSPAKLEAFIKEHERKLLATQQAQMVQQQQQQMQQVASVTGLHNQ 518          
BLAST of Transcriptional repressor p66-beta vs. TrEMBL
Match: A0A183MMK6 (GATA-type domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS17281 PE=4 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 1.592e-52
Identity = 125/356 (35.11%), Postives = 191/356 (53.65%), Query Frame = 3
Query:  102 AMDLSRKVNSQGSGVICLSEDEDDENINQLLTKLNIE-KPSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKI---PPST--SPANTSTIRQNGHVAQXXXXXXXXXXXXXXXXXXXXXXXXXXETSIENSPNNQTDAQAVPGNRTSAHGASNNTTAPYTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLL 1151
             +DLSRK N     VI LSEDEDD  +  L  ++  E  P    +   +  L +L+  LRNEE+  +LL Q++  Q+     P ST  S + T+T+R    V+Q +                           I + P+  T  +A     T+A   +N T                ++T+ MA+ AL+QQ   +KA+LRKQ ++ L +V L +P  G    S +  +PST TNEF A++GLEE+V  +Q++  L+   ++ + R V  PF+C +CGTDFTP+WKR+RPGSS+VVCE+C  ++ R  +   Y   I   LK++   ER+++ +Y+D+++SP K E F KE E+KLL
Sbjct:   24 VLDLSRKSNHNDDDVIMLSEDEDDRLLGSLCDEMEKENAPKQSLLSKSRAFLLKLENELRNEESALVLLQQLRANQRSHALQPKSTKVSSSVTNTVRIRPAVSQAV---------------------------IRSVPST-TGGRATLSQNTAAVTPTNATAIT-------------KVTKGMAIHALEQQCSGKKAMLRKQLERTLDKVVLPKPAVGN-GLSVVPFVPSTLTNEFTAMIGLEEIVTTIQDFDPLSTDKSSEQSRFV-NPFSCSRCGTDFTPVWKRKRPGSSEVVCEACIVDSQRSVIHKAYNSVISAALKQHAASEREIEHEYQDVVNSPAKLEAFIKEHERKLL 336          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Cavefish
Match: GATAD2A (GATA zinc finger domain containing 2A [Source:HGNC Symbol;Acc:HGNC:29989])

HSP 1 Score: 91.2781 bits (225), Expect = 6.420e-19
Identity = 50/148 (33.78%), Postives = 79/148 (53.38%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSP 1106
            A K  LRKQ +K L E+P            + N +PS A NEF+ LVGLEEVV+ L +    A+  +         PF C QC TDFT  W++E+ G+  ++CE C   N ++AL+ ++ ++++    K   QE+++++       SP
Sbjct:  311 AAKLALRKQLEKTLLEIPPP-----KPPAPEFNFLPSAANNEFIYLVGLEEVVQCLLDTLGRAVAPS--------EPFTCTQCKTDFTCRWRKEKSGA--IMCEQCMSSNQKKALKAEHTNRLKAAFVKALQQEQEIEQRILQQAASP 443          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Cavefish
Match: gatad2b (GATA zinc finger domain containing 2B [Source:ZFIN;Acc:ZDB-GENE-060503-655])

HSP 1 Score: 90.5077 bits (223), Expect = 1.250e-18
Identity = 57/179 (31.84%), Postives = 99/179 (55.31%), Query Frame = 3
Query:  555 SNNTTAPYTNESNKSSS--NGPRMTREMAMQALDQQY---LARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLD 1076
            SN++TA Y N ++  S+  +G  +    ++ +L+ Q     A K  LRKQ +K L E+P             L+ +PS A +EF+ +VGLEEVV+++ + +    G  +        PF C QC TDFTP WK+E+ G  +++CE C   N ++AL+ ++ ++++    K   QE++++
Sbjct:  318 SNSSTAIYMNLAHIPSTLPSGSSVAAVGSVSSLNDQASSQAAAKLALRKQLEKTLLEIPPP-----KPPAPLLHFLPSAANSEFIYMVGLEEVVQSVIDSQGKMRGSLSR-----LEPFFCAQCRTDFTPHWKQEKSG--RILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIE 484          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Cavefish
Match: gatad2ab (transcriptional repressor p66-alpha-like [Source:NCBI gene;Acc:111192506])

HSP 1 Score: 89.3521 bits (220), Expect = 3.193e-18
Identity = 49/142 (34.51%), Postives = 81/142 (57.04%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFT---PFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            +LN +PS A NEF+ LVGLE VV+NL     L L  +  + +   +   P+ C QC TDFT  W++E+ G++ ++CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  324 AAKLALRKQLEKTLLEIPPP-----KPPAPELNFLPSAANNEFIYLVGLEMVVQNL-----LDLAKSKQQAQQGTSAKDPYTCAQCQTDFTSRWRQEK-GNNTILCEQCMSSNQKKALKAEHTNRLKAAFVKALQQEQEIEQ 454          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Cavefish
Match: gatad2ab (transcriptional repressor p66-alpha-like [Source:NCBI gene;Acc:111192506])

HSP 1 Score: 89.3521 bits (220), Expect = 3.646e-18
Identity = 49/142 (34.51%), Postives = 81/142 (57.04%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFT---PFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            +LN +PS A NEF+ LVGLE VV+NL     L L  +  + +   +   P+ C QC TDFT  W++E+ G++ ++CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  339 AAKLALRKQLEKTLLEIPPP-----KPPAPELNFLPSAANNEFIYLVGLEMVVQNL-----LDLAKSKQQAQQGTSAKDPYTCAQCQTDFTSRWRQEK-GNNTILCEQCMSSNQKKALKAEHTNRLKAAFVKALQQEQEIEQ 469          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Cavefish
Match: GATAD2A (GATA zinc finger domain containing 2A [Source:HGNC Symbol;Acc:HGNC:29989])

HSP 1 Score: 88.9669 bits (219), Expect = 4.389e-18
Identity = 48/140 (34.29%), Postives = 76/140 (54.29%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLA-LGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            + N +PS A NEF+ LVGLEEVV+ L +      +G           PF C QC TDFT  W++E+ G+  ++CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  367 AAKLALRKQLEKTLLEIPPP-----KPPAPEFNFLPSAANNEFIYLVGLEEVVQCLLDTLGRGKMGGAFPSAVAPSEPFTCTQCKTDFTCRWRKEKSGA--IMCEQCMSSNQKKALKAEHTNRLKAAFVKALQQEQEIEQ 499          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Sea Lamprey
Match: gatad2ab (GATA zinc finger domain containing 2Ab [Source:ZFIN;Acc:ZDB-GENE-040426-2372])

HSP 1 Score: 91.2781 bits (225), Expect = 9.749e-20
Identity = 49/129 (37.98%), Postives = 73/129 (56.59%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKK 1049
            A K  LRKQ +K L E+P            +LN +PS A +EFV L+GLEEVV++L E +  A         M   PF C+QC TDFT  W+ E+ G+  V+CE C   N ++AL+ ++ +++++   K
Sbjct:  240 AAKLALRKQLEKTLLEIPPP-----KPPPPELNFLPSAANSEFVYLIGLEEVVQSLLETQGKAAPQP-----MGREPFKCLQCQTDFTARWRHEKGGT--VICEQCLSSNQKKALKAEHTNRLKMAFVK 356          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Nematostella
Match: EDO32520 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUU6])

HSP 1 Score: 69.707 bits (169), Expect = 1.903e-12
Identity = 49/161 (30.43%), Postives = 78/161 (48.45%), Query Frame = 3
Query:  603 SNGP-RMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVV-CESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            S GP +     AM A  Q   A K  LRKQ +K L ++P            + + +PS  + EF+ LVG E VV  L   +++  G        + +P  C QCGTDFTP WK +      V+ CE C   N ++ L+ ++  +++    K   QE+++++
Sbjct:  447 STGPNKACLSQAMSAGKQ--AAAKMALRKQLEKTLLQIPPP-----KPPPPEWSFLPSLNSTEFMTLVGFEMVVDVLAHKKSVTGGSE------LSSPSLCGQCGTDFTPGWKAKPKNKEGVLMCEKCSTVNVKKELKAEHTARLKAAFLKALKQEQEIEQ 594          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Medaka
Match: ENSORLT00000019345.2 (transcriptional repressor p66-alpha [Source:NCBI gene;Acc:101173502])

HSP 1 Score: 87.8113 bits (216), Expect = 1.219e-17
Identity = 47/139 (33.81%), Postives = 75/139 (53.96%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            + N +PS A NEF+ LVGLEEVV+NL +  +     +         PF C QC TDFT  W++++     V+CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  415 AAKLALRKQLEKTLLEIPPP-----KPPAPEFNFLPSAANNEFIYLVGLEEVVQNLLDTIHRGKTGSLPTKLGNREPFICSQCNTDFTCRWRQDKAKGGVVLCEDCMSSNQKKALKAEHTNRLKAAFVKALQQEQEIEQ 548          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Medaka
Match: gatad2b (GATA zinc finger domain containing 2B [Source:NCBI gene;Acc:101174035])

HSP 1 Score: 87.0409 bits (214), Expect = 1.717e-17
Identity = 48/137 (35.04%), Postives = 79/137 (57.66%), Query Frame = 3
Query:  669 KALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            K  LRKQ +K L E+P             L+ +PS A +EF+ +VGLEEVV+++ + +    G      RM   PF C QC TDFTP WK+E+ G  +++CE C   N ++AL+ ++ ++++    K   QE+++++
Sbjct:  384 KLALRKQLEKTLLEIPPP-----KPPAPLLHFLPSAANSEFIYMVGLEEVVQSVLDSQGKLRG---ALARM--EPFFCAQCRTDFTPHWKQEKSG--RILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIEQ 508          
BLAST of Transcriptional repressor p66-beta vs. Ensembl Medaka
Match: gatad2ab (transcriptional repressor p66-alpha [Source:NCBI gene;Acc:101167110])

HSP 1 Score: 86.2705 bits (212), Expect = 3.330e-17
Identity = 47/141 (33.33%), Postives = 75/141 (53.19%), Query Frame = 3
Query:  663 ARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLA-LGHNTNEVRMVFT-PFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDR 1079
            A K  LRKQ +K L E+P            + N +PS A NEF+ L+GLEEVV+ L E      LG  T     +   P  C QC TDFT  W++E+ G+  ++C+ C   N ++ L+ ++  +++    K   QE+++++
Sbjct:  341 AAKLALRKQLEKTLLEIPPP-----KPPAPEFNFLPSAANNEFIYLLGLEEVVQKLLEMHGRGNLGPATAMANSISKEPHTCAQCKTDFTSRWRKEKAGT--ILCDQCMSSNQKKVLKAEHTSRLKSAFVKALQQEQEIEQ 474          
BLAST of Transcriptional repressor p66-beta vs. Planmine SMEST
Match: SMESG000077622.1 (SMESG000077622.1)

HSP 1 Score: 1016.14 bits (2626), Expect = 0.000e+0
Identity = 578/581 (99.48%), Postives = 580/581 (99.83%), Query Frame = 3
Query:   36 MEIEVLDLSQKPICLNENDLPTAMDLSRKVNSQGSGVICLSEDEDDENINQLLTKLNIEKPSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKIPPSTSPANTSTIRQNGHVAQXXXXXXXXXXXXXXXXXXXXXXXXXXETSIENSPNNQTDAQAVPGNRTSAHGASNNTTAPYTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQTPTSTVTATQSQGRPSHRPSNTVSTVSGAARTAPTTVSGGYGQQQQMRHQNXXXXXXXXXXXXXXQLTVDQLAYMQMFQALNQLGSSSNXXXXXXXXXXXXXXXXXXMAYMSSASTLLGGSTNNAAANSFVQFLSNMAGLLNNPSSTPETTXXXXXXXXXXXXXXXXXXMLQQQQATANSDK 1778
            MEIEVLDLSQKPICLNENDLPTAMDLSRKVNSQGSGVICLSEDEDDENINQLLTKLNIEKPSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKIPPSTSPANTSTIRQNGHVAQPI+NNNISNTTNNNNPISQNSNRPNNETSIENSPNNQTDAQAVPGNRTSAHGASNNTTAPYTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQTPTSTVTATQSQGRPSHRPSNTVSTVSGAARTAPTTVSGGYGQQQQMRHQNTAASGISA STAPSQLTVDQLAYMQMFQALNQLGSSSNLNQQATRQQQQLMQQILQMAYMSSASTLLGGSTNNAAANSFVQFLSNMAGLLNNPSSTP+TTGASANSGNNNALLNYYLSMLQQQQATANSDK
Sbjct:    1 MEIEVLDLSQKPICLNENDLPTAMDLSRKVNSQGSGVICLSEDEDDENINQLLTKLNIEKPSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKIPPSTSPANTSTIRQNGHVAQPISNNNISNTTNNNNPISQNSNRPNNETSIENSPNNQTDAQAVPGNRTSAHGASNNTTAPYTNESNKSSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQTPTSTVTATQSQGRPSHRPSNTVSTVSGAARTAPTTVSGGYGQQQQMRHQNTAASGISAPSTAPSQLTVDQLAYMQMFQALNQLGSSSNLNQQATRQQQQLMQQILQMAYMSSASTLLGGSTNNAAANSFVQFLSNMAGLLNNPSSTPDTTGASANSGNNNALLNYYLSMLQQQQATANSDK 581          
BLAST of Transcriptional repressor p66-beta vs. Planmine SMEST
Match: SMESG000064553.1 (SMESG000064553.1)

HSP 1 Score: 118.627 bits (296), Expect = 4.542e-28
Identity = 63/185 (34.05%), Postives = 108/185 (58.38%), Query Frame = 3
Query:  597 SSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSS--KVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKK 1145
            S S+   +T EM+ + LD Q++ R+ ++  Q +K L E+P+ R       Q     IP+T+  EF+ALVG  +V+  L+E +N     +T+E   V T   C  C ++ + +WK+ +  +   + +C +C  E  +   Q D +++I+ +  ++ +QE++L RDY DML SPKK E+FR+EQEK+
Sbjct:  114 SKSDEFFLTLEMSRKLLDHQFITRQKMINSQLEKTLYEIPIKR----QQVQYTNEFIPNTSNPEFIALVGFSQVIDVLKEQQN-ENPESTDESSFVDTIDVCHNCQSEASSVWKKRKISNDIEETICSTCNNEINQSQNQKDLEEQIKKIKMQFDEQEKNLIRDYNDMLSSPKKLEMFRQEQEKR 293          
BLAST of Transcriptional repressor p66-beta vs. Planmine SMEST
Match: SMESG000064553.1 (SMESG000064553.1)

HSP 1 Score: 117.087 bits (292), Expect = 1.276e-27
Identity = 61/185 (32.97%), Postives = 107/185 (57.84%), Query Frame = 3
Query:  597 SSSNGPRMTREMAMQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATNEFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLWKRERPGSS--KVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDYKDMLDSPKKFELFRKEQEKK 1145
            S S+   +T EM+ + LD Q++ R+ ++  Q +K L E+P+ R       Q     IP+T+  EF+ALVG  +V+  L++ +      +T+E   V T   C  C ++ + +WK+ +  +   + +C +C  E  +   Q D +++I+ +  ++ +QE++L RDY DML SPKK E+FR+EQEK+
Sbjct:  114 SKSDEFFLTLEMSRKLLDHQFITRQKMINSQLEKTLYEIPIKR----QQVQYTNEFIPNTSNPEFIALVGFSQVIDVLKQEQQNENPESTDESSFVDTIDVCHNCQSEASSVWKKRKISNDIEETICSTCNNEINQSQNQKDLEEQIKKIKMQFDEQEKNLIRDYNDMLSSPKKLEMFRQEQEKR 294          
The following BLAST results are available for this feature:
BLAST of Transcriptional repressor p66-beta vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GATAD2B9.405e-1932.49GATA zinc finger domain containing 2B [Source:HGNC... [more]
GATAD2A1.044e-1835.51GATA zinc finger domain containing 2A [Source:HGNC... [more]
GATAD2A1.044e-1835.51GATA zinc finger domain containing 2A [Source:HGNC... [more]
GATAD2A1.093e-1835.51GATA zinc finger domain containing 2A [Source:HGNC... [more]
GATAD2B7.937e-1735.51GATA zinc finger domain containing 2B [Source:HGNC... [more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dcp-663.842e-625.00Deacetylase Complex Protein [Source:UniProtKB/TrE... [more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
simj4.235e-1630.57gene:FBgn0010762 transcript:FBtr0331573[more]
simj4.352e-1630.57gene:FBgn0010762 transcript:FBtr0076264[more]
simj4.555e-1630.57gene:FBgn0010762 transcript:FBtr0076263[more]
simj4.555e-1630.57gene:FBgn0010762 transcript:FBtr0076262[more]
simj4.734e-1631.03gene:FBgn0010762 transcript:FBtr0331574[more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gatad2ab2.790e-1733.11GATA zinc finger domain containing 2Ab [Source:ZFI... [more]
gatad2ab2.790e-1733.11GATA zinc finger domain containing 2Ab [Source:ZFI... [more]
gatad2ab2.826e-1733.11GATA zinc finger domain containing 2Ab [Source:ZFI... [more]
gatad2b2.842e-1734.06GATA zinc finger domain containing 2B [Source:ZFIN... [more]
gatad2ab4.361e-1733.11GATA zinc finger domain containing 2Ab [Source:ZFI... [more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rab321.298e-1829.79RAB32, member RAS oncogene family [Source:Xenbase;... [more]
rab322.094e-1829.79RAB32, member RAS oncogene family [Source:Xenbase;... [more]
GATAD2B1.434e-1735.97GATA zinc finger domain containing 2B [Source:NCBI... [more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gatad2b2.197e-1935.97GATA zinc finger domain containing 2B [Source:MGI ... [more]
Gatad2a2.309e-1936.69GATA zinc finger domain containing 2A [Source:MGI ... [more]
Gatad2a3.719e-1936.69GATA zinc finger domain containing 2A [Source:MGI ... [more]
Gatad2a3.777e-1936.69GATA zinc finger domain containing 2A [Source:MGI ... [more]
Gatad2a3.777e-1936.69GATA zinc finger domain containing 2A [Source:MGI ... [more]
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BLAST of Transcriptional repressor p66-beta vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q8CHY6|P66A_MOUSE2.642e-1836.69Transcriptional repressor p66 alpha OS=Mus musculu... [more]
sp|Q86YP4|P66A_HUMAN5.013e-1835.51Transcriptional repressor p66-alpha OS=Homo sapien... [more]
sp|Q8VHR5|P66B_MOUSE8.452e-1735.97Transcriptional repressor p66-beta OS=Mus musculus... [more]
sp|Q8WXI9|P66B_HUMAN3.811e-1635.51Transcriptional repressor p66-beta OS=Homo sapiens... [more]
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BLAST of Transcriptional repressor p66-beta vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Z2DRT66.359e-5534.26Transcriptional repressor p66-beta isoform 2 OS=Sc... [more]
A0A504Z1B51.761e-5436.13Transcriptional repressor p66-beta OS=Fasciola gig... [more]
A0A4Z2DSZ61.111e-5334.26Transcriptional repressor p66-beta isoform 3 OS=Sc... [more]
A0A4Z2DS261.482e-5334.26Transcriptional repressor p66-beta isoform 1 OS=Sc... [more]
A0A183MMK61.592e-5235.11GATA-type domain-containing protein OS=Schistosoma... [more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GATAD2A6.420e-1933.78GATA zinc finger domain containing 2A [Source:HGNC... [more]
gatad2b1.250e-1831.84GATA zinc finger domain containing 2B [Source:ZFIN... [more]
gatad2ab3.193e-1834.51transcriptional repressor p66-alpha-like [Source:N... [more]
gatad2ab3.646e-1834.51transcriptional repressor p66-alpha-like [Source:N... [more]
GATAD2A4.389e-1834.29GATA zinc finger domain containing 2A [Source:HGNC... [more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gatad2ab9.749e-2037.98GATA zinc finger domain containing 2Ab [Source:ZFI... [more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO325201.903e-1230.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transcriptional repressor p66-beta vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000019345.21.219e-1733.81transcriptional repressor p66-alpha [Source:NCBI g... [more]
gatad2b1.717e-1735.04GATA zinc finger domain containing 2B [Source:NCBI... [more]
gatad2ab3.330e-1733.33transcriptional repressor p66-alpha [Source:NCBI g... [more]
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BLAST of Transcriptional repressor p66-beta vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000077622.10.000e+099.48SMESG000077622.1[more]
SMESG000064553.14.542e-2834.05SMESG000064553.1[more]
SMESG000064553.11.276e-2732.97SMESG000064553.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010167 ID=SMED30010167|Name=Transcriptional repressor p66-beta|organism=Schmidtea mediterranea sexual|type=transcript|length=1869bp
CTATATAAATCTTATATGAAATTATTTTAGTTTAAATGGAAATCGAAGTA
CTTGATTTAAGTCAAAAACCAATTTGTTTAAATGAAAATGATTTACCCAC
TGCAATGGATCTATCAAGAAAAGTTAATTCACAAGGCTCTGGTGTAATAT
GCTTGAGTGAGGATGAAGATGATGAAAATATTAATCAATTATTGACCAAA
CTTAACATCGAAAAACCCTCAATGAAAACGATTGAAGCTTGTCAATTATT
ATTGCATCAATTAGATGAAAGGCTTCGCAATGAAGAAACAATTGAAATTC
TTTTGGCCCAAATGAAGCATACTCAAAAGAAAATTCCACCGTCAACAAGC
CCGGCTAATACTAGTACTATTCGGCAAAATGGTCATGTGGCTCAACCAAT
TACTAATAACAATATTAGCAATACTACAAATAATAATAATCCAATCAGTC
AAAATTCCAATAGACCAAATAACGAAACAAGCATAGAAAACTCCCCAAAT
AATCAGACAGATGCTCAGGCAGTACCTGGTAATCGAACAAGTGCTCACGG
TGCGTCAAACAACACCACTGCTCCGTACACAAATGAATCAAACAAATCAT
CATCTAATGGTCCACGAATGACTCGAGAAATGGCTATGCAAGCTCTTGAT
CAACAATATTTAGCCAGGAAAGCTTTATTACGCAAGCAAGCCGACAAGGC
TTTATCTGAAGTACCTTTGGCAAGACCACCACAAGGTGCAATGGCCCAAT
CAGATTTGAACTTGATTCCAAGCACTGCAACCAATGAATTTGTCGCTCTT
GTTGGATTGGAGGAGGTCGTCAAAAATCTTCAGGAATATCGTAATTTGGC
GCTCGGTCATAATACAAATGAAGTCCGTATGGTATTCACACCGTTCAATT
GTGTTCAATGTGGCACAGACTTTACACCCCTCTGGAAACGAGAACGGCCC
GGTAGTAGCAAAGTTGTTTGTGAATCGTGTTGTGGTGAAAATTATCGTCG
AGCTCTCCAAAATGATTATCAAGATAAAATTCGGCTCGTTCTCAAAAAAT
ATCAAGATCAAGAACGTGATTTGGATCGCGATTATAAAGACATGCTTGAT
AGTCCTAAAAAATTTGAATTATTCCGTAAAGAACAAGAAAAAAAACTGTT
GGCCATGAGTACCACTAACAACCGTCCTAACGCTACAACGACACAACCTG
GATCTCGTACATCTACAGGGCATCATCATCAAACACCAACGAGCACTGTG
ACAGCAACACAATCGCAGGGTCGGCCTTCACATCGGCCATCAAATACGGT
GTCGACGGTTAGTGGCGCCGCCAGAACAGCACCAACAACGGTTTCTGGAG
GATATGGTCAACAGCAACAAATGCGACATCAGAATACAGCAGCTAGTGGT
ATTAGTGCTACATCAACTGCTCCATCTCAGTTAACTGTGGATCAATTGGC
ATACATGCAAATGTTTCAAGCACTTAATCAACTCGGTTCATCAAGTAATT
TGAATCAACAAGCTACCCGCCAACAACAACAGCTCATGCAACAAATTCTT
CAGATGGCTTATATGAGTAGTGCAAGCACTCTTCTTGGTGGATCTACAAA
CAATGCAGCTGCAAATTCATTCGTACAATTTCTTAGCAACATGGCAGGTC
TCCTCAATAATCCCTCAAGTACCCCTGAAACAACTGGCGCCAGCGCTAAC
AGCGGTAATAATAACGCTCTATTGAACTACTATCTATCGATGCTGCAGCA
ACAACAAGCAACAGCAAATTCTGACAAATGATTCTTTTGGTAGTCCTCCA
AGGATGTCCCATTGACTCATGCGTATTTATTTGTGTACAAGTCTATTTGT
TTTCTACAGATCGGAAGAG
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protein sequence of SMED30010167-orf-1

>SMED30010167-orf-1 ID=SMED30010167-orf-1|Name=SMED30010167-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=582bp
MEIEVLDLSQKPICLNENDLPTAMDLSRKVNSQGSGVICLSEDEDDENIN
QLLTKLNIEKPSMKTIEACQLLLHQLDERLRNEETIEILLAQMKHTQKKI
PPSTSPANTSTIRQNGHVAQPITNNNISNTTNNNNPISQNSNRPNNETSI
ENSPNNQTDAQAVPGNRTSAHGASNNTTAPYTNESNKSSSNGPRMTREMA
MQALDQQYLARKALLRKQADKALSEVPLARPPQGAMAQSDLNLIPSTATN
EFVALVGLEEVVKNLQEYRNLALGHNTNEVRMVFTPFNCVQCGTDFTPLW
KRERPGSSKVVCESCCGENYRRALQNDYQDKIRLVLKKYQDQERDLDRDY
KDMLDSPKKFELFRKEQEKKLLAMSTTNNRPNATTTQPGSRTSTGHHHQT
PTSTVTATQSQGRPSHRPSNTVSTVSGAARTAPTTVSGGYGQQQQMRHQN
TAASGISATSTAPSQLTVDQLAYMQMFQALNQLGSSSNLNQQATRQQQQL
MQQILQMAYMSSASTLLGGSTNNAAANSFVQFLSNMAGLLNNPSSTPETT
GASANSGNNNALLNYYLSMLQQQQATANSDK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0000122negative regulation of transcription from RNA polymerase II promoter
GO:0006355regulation of transcription, DNA-templated
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0046872metal ion binding
GO:0008270zinc ion binding
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
GO:0003676nucleic acid binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000429neoblast
PLANA:0002111X2 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR040386P66
IPR013088Znf_NHR/GATA
IPR000679Znf_GATA
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 374..461
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 120..193
NoneNo IPR availablePANTHERPTHR13455:SF7SIMJANG, ISOFORM Ecoord: 202..545
IPR040386Transcriptional repressor p66PANTHERPTHR13455TRANSCRIPTIONAL REPRESSOR P66-RELATEDcoord: 202..545
IPR000679Zinc finger, GATA-typePROSITEPS50114GATA_ZN_FINGER_2coord: 289..315
score: 8.853