SMED30010144

Overview
NameSMED30010144
Smed IDSMED30010144
Length (bp)1958
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010144 (SMED30010144) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010144 (SMED30010144) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010144 (SMED30010144) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010144 (SMED30010144) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010144

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30010144SMESG000038665.1 dd_Smed_v4_6969_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010144SMESG000038665.1 dd_Smed_v4_6969_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30010144SMESG000038665.1 dd_Smed_v4_6969_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010144SMESG000038665.1 dd_Smed_v4_6969_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30010144 vs. Ensembl Fly
Match: pck (gene:FBgn0013720 transcript:FBtr0070256)

HSP 1 Score: 61.2326 bits (147), Expect = 4.729e-10
Identity = 60/247 (24.29%), Postives = 107/247 (43.32%), Query Frame = 3 
Query:  147 RLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEI-YDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYIS--LMLAALICTLMSLGLMNLRHFKNVIVLI--LLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQFELESVHTMMGPSIYDPRGTSSASK 872
            R  N  +F A  I T  S+    ++F    W   +  + + F+N+GLW+ CF  F+  +  + K F+ C  I S +   +  +++  W +A Q   T+S+I   L+LA L  T++ L L  +  ++ ++V +  +   I+ +  F  + +FG          WM  P    L WSY  A    + T + LGL   +++              + +Q+    Q E++       P    PR   S S+
Sbjct:   24 RATNGVVFGA--IVTFASFFVLMMSFCSPYWIESYEETRASFKNMGLWQYCFKDFVYPKYAFLKQFTGCHNIFSHEYYVIREYLLPGWLMAVQGFVTMSFIIVFLVLALLSLTIIRLPLKAVLQYEWLLVRLSYMGTAISSLFMFLAVCIFGGCAYRRD---WMMYPKFNVLGWSYALA----VVTFMLLGLAALILQREARQAYD-----ARGEQKNLVMQMEMQE------PGYQPPRHHHSQSR 250
[Close]
BLAST of SMED30010144 vs. Ensembl Fly
Match: pck (gene:FBgn0013720 transcript:FBtr0070255)

HSP 1 Score: 61.2326 bits (147), Expect = 4.729e-10
Identity = 60/247 (24.29%), Postives = 107/247 (43.32%), Query Frame = 3 
Query:  147 RLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEI-YDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYIS--LMLAALICTLMSLGLMNLRHFKNVIVLI--LLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQFELESVHTMMGPSIYDPRGTSSASK 872
            R  N  +F A  I T  S+    ++F    W   +  + + F+N+GLW+ CF  F+  +  + K F+ C  I S +   +  +++  W +A Q   T+S+I   L+LA L  T++ L L  +  ++ ++V +  +   I+ +  F  + +FG          WM  P    L WSY  A    + T + LGL   +++              + +Q+    Q E++       P    PR   S S+
Sbjct:   24 RATNGVVFGA--IVTFASFFVLMMSFCSPYWIESYEETRASFKNMGLWQYCFKDFVYPKYAFLKQFTGCHNIFSHEYYVIREYLLPGWLMAVQGFVTMSFIIVFLVLALLSLTIIRLPLKAVLQYEWLLVRLSYMGTAISSLFMFLAVCIFGGCAYRRD---WMMYPKFNVLGWSYALA----VVTFMLLGLAALILQREARQAYD-----ARGEQKNLVMQMEMQE------PGYQPPRHHHSQSR 250
[Close]
BLAST of SMED30010144 vs. Ensembl Fly
Match: kune (gene:FBgn0033032 transcript:FBtr0086037)

HSP 1 Score: 56.9954 bits (136), Expect = 1.247e-8
Identity = 40/183 (21.86%), Postives = 89/183 (48.63%), Query Frame = 3 
Query:  183 IFTMISYL--FSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMN-EEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYI-SLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFV 719
            +F++I ++  FST  ++V+      P     F N+GLWEVCFN+F +    +D  F+ C ++   +   ++++++  ++++ Q+  TL ++  L++  L    +     + R+   ++ +     +  V  F  +++FG    +     WMP      + WS+    +  +  + S G+L+ V
Sbjct:   16 VFSLICFVIAFSTPYWLVTDGRLQNP----RFTNLGLWEVCFNNFQDIHRFFDNSFNGCLWVFEEEYYIIHDFLLPGFYISVQLFATLCFVMCLVVIPLTVAFLRTSRDDDRYMVLLLAIGSCQVVGSVFGFIAVVIFGAKGDSRD---WMPGWQNNDMGWSFALGVVGAVLLLPS-GVLYLV 190
[Close]
BLAST of SMED30010144 vs. Ensembl Fly
Match: sinu (gene:FBgn0010894 transcript:FBtr0077136)

HSP 1 Score: 48.9062 bits (115), Expect = 6.039e-6
Identity = 39/185 (21.08%), Postives = 86/185 (46.49%), Query Frame = 3 
Query:  171 VALFIFTMISYLFSTLAFIVSQW-YSMFPGSFSDFQNIGLWEVCFNSF--MNEEIYDKLFSDCFYIS---SSDISDLYNWIVTNWFLACQVIYTLSYISLMLAAL-ICTLMSLGLMNLRHFKNVI--VLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVS 698
            V +F+F   ++ F  +AF    W  S +  + +    +GLW  CF S   +N++   + F  C ++    ++   ++  +++  + +A Q  YTL++I ++++A+ +   +     + +HF  +I  +  +L         A ++  G   +N     WMP+       WS+  A +  + T+V+
Sbjct:   40 VGVFVF---AFAFIVIAFATPSWLVSDYRITGAKLDRLGLWVHCFRSLPDVNDDSQRRFFVGCRWVYDPFTTGYDEIRGFLLPAFMIATQFFYTLAFIGMLVSAIGVLVFILCAGPDQKHFITLIKSLGYVLLGAGVSAAIAVIVFAGFGNRN----GWMPEHANNWFGWSFILACVGTVLTLVA 217
[Close]
BLAST of SMED30010144 vs. TrEMBL
Match: A0A4E0R425 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_005990 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 3.394e-23
Identity = 59/204 (28.92%), Postives = 102/204 (50.00%), Query Frame = 3 
Query:  144 NRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNT------KNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRN 737
            N     PL     +FT    L S +AF++  WY+ +P S S F  +GLWE+CF  FM  ++ + +++ CFY+    I  L++ I   WF+ CQV +T S+++ ++  ++       L++ R  + V+  I+      +    + +  G         K  +E  W+    QC+LSW+YG + LSLL  +V   +L + V   R+
Sbjct:    4 NGKQRRPLLWTCLLFTTFGLLCSVVAFVLPYWYARYPQSKSRFLRLGLWEICFEDFMPADLGNLMYTGCFYLYDRKIRALWSLIFRTWFVVCQVFHTFSFVAYVMVEVVLITQVCNLVSSRGPRAVLFSIIASGATLLFVLISSVTMGTGVDAESKRKPSAEQDWLISLDQCSLSWAYGISLLSLLGCLVPFVILCWCVYPKRS 207
[Close]
BLAST of SMED30010144 vs. TrEMBL
Match: A0A504YNA1 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_00762 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 4.461e-23
Identity = 61/204 (29.90%), Postives = 102/204 (50.00%), Query Frame = 3 
Query:  144 NRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLI------LLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRN 737
            N     PL     +FT    L S +AF++  WY+ +P S S F  +GLWE+CF  FM  ++ + +++ CFY+    I  L++ I   WF+ CQV +T S+++ ++  ++       L++ R  + V+  I      LL  +       T +      K  +E  W+    QC+LSW+YG + LSLL  +V   +L + V   R+
Sbjct:    4 NGKQRRPLLWTCLLFTTFGLLCSVVAFVLPYWYARYPQSKSRFLRLGLWEICFEDFMPADLGNLMYTGCFYLYDRKIRALWSLIFRTWFVVCQVFHTFSFVAYVMVEVVLITQVCNLVSSRGPRAVLFSIIASGATLLFVLIGSVTMGTGVDAESKRKPSAEQDWLISLDQCSLSWAYGISLLSLLGCLVPFVILCWCVYPKRS 207
[Close]
BLAST of SMED30010144 vs. TrEMBL
Match: A0A4Z2DDK4 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002054 PE=4 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.934e-22
Identity = 57/184 (30.98%), Postives = 104/184 (56.52%), Query Frame = 3 
Query:  210 STLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQ------WMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNC 743
            S ++ ++  WY+ +P S + F  +GLWEVCF+ +M ++  + ++  CFY+  + I  L+  I  +WF+ACQ+ +T S+ + +LA  +    + GL + R  + V+ +++   I + +   +LI  GV      + +      W+   +Q +LSWSYG AS+SLL T +++ LL + V   R+ C
Sbjct:   24 SFVSMVMPYWYARYPQSQNRFVRLGLWEVCFDGYMAKKPGNNMYMGCFYLFDTKIQTLWPIIFRDWFIACQISFTCSFATYILAECVILTQAFGLFSFRGSRVVLCMMVALSINYFSTLISLITMGVGVDTEKKIESTAKCPWLEYLSQSSLSWSYGLASVSLLPTWITIILLGWFVYPKRSPC 207
[Close]
BLAST of SMED30010144 vs. TrEMBL
Match: A0A4Z2DEH4 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002054 PE=4 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 3.742e-22
Identity = 57/184 (30.98%), Postives = 104/184 (56.52%), Query Frame = 3 
Query:  210 STLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQ------WMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNC 743
            S ++ ++  WY+ +P S + F  +GLWEVCF+ +M ++  + ++  CFY+  + I  L+  I  +WF+ACQ+ +T S+ + +LA  +    + GL + R  + V+ +++   I + +   +LI  GV      + +      W+   +Q +LSWSYG AS+SLL T +++ LL + V   R+ C
Sbjct:   24 SFVSMVMPYWYARYPQSQNRFVRLGLWEVCFDGYMAKKPGNNMYMGCFYLFDTKIQTLWPIIFRDWFIACQISFTCSFATYILAECVILTQAFGLFSFRGSRVVLCMMVALSINYFSTLISLITMGVGVDTEKKIESTAKCPWLEYLSQSSLSWSYGLASVSLLPTWITIILLGWFVYPKRSPC 207
[Close]
BLAST of SMED30010144 vs. TrEMBL
Match: A0A4Z2DDP7 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002054 PE=4 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 4.561e-22
Identity = 57/184 (30.98%), Postives = 104/184 (56.52%), Query Frame = 3 
Query:  210 STLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQ------WMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNC 743
            S ++ ++  WY+ +P S + F  +GLWEVCF+ +M ++  + ++  CFY+  + I  L+  I  +WF+ACQ+ +T S+ + +LA  +    + GL + R  + V+ +++   I + +   +LI  GV      + +      W+   +Q +LSWSYG AS+SLL T +++ LL + V   R+ C
Sbjct:   24 SFVSMVMPYWYARYPQSQNRFVRLGLWEVCFDGYMAKKPGNNMYMGCFYLFDTKIQTLWPIIFRDWFIACQISFTCSFATYILAECVILTQAFGLFSFRGSRVVLCMMVALSINYFSTLISLITMGVGVDTEKKIESTAKCPWLEYLSQSSLSWSYGLASVSLLPTWITIILLGWFVYPKRSPC 207
[Close]
BLAST of SMED30010144 vs. Planmine SMEST
Match: SMESG000038665.1 (SMESG000038665.1)

HSP 1 Score: 657.522 bits (1695), Expect = 0.000e+0
Identity = 355/375 (94.67%), Postives = 356/375 (94.93%), Query Frame = 3 
Query:  141 MNRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQFELESVHTMMGPSIYDPRGTSSASKISAEXXXXXXXXXXXXTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQTNKNPSQASLLSDKPTXXXXXXXXXXXXXXXXXLGHEKITPGFDKPGKISSPTASQLTLTARNQQPSSSIVI 1265
            MNRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQ ELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSK                  TNKNPSQASLLSDKPTSSLYSKRSKQSSHSNPSLGHEKITPGFDKPGKISSPTASQLTLTA+NQQPSSSIVI
Sbjct:    1 MNRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQLELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSK------------------TNKNPSQASLLSDKPTSSLYSKRSKQSSHSNPSLGHEKITPGFDKPGKISSPTASQLTLTAKNQQPSSSIVI 357
[Close]
BLAST of SMED30010144 vs. Planmine SMEST
Match: SMESG000038665.1 (SMESG000038665.1)

HSP 1 Score: 622.854 bits (1605), Expect = 0.000e+0
Identity = 338/358 (94.41%), Postives = 339/358 (94.69%), Query Frame = 3 
Query:  192 MISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQFELESVHTMMGPSIYDPRGTSSASKISAEXXXXXXXXXXXXTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQTNKNPSQASLLSDKPTXXXXXXXXXXXXXXXXXLGHEKITPGFDKPGKISSPTASQLTLTARNQQPSSSIVI 1265
            MISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQ ELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSK                  TNKNPSQASLLSDKPTSSLYSKRSKQSSHSNPSLGHEKITPGFDKPGKISSPTASQLTLTA+NQQPSSSIVI
Sbjct:    1 MISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQLELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSK------------------TNKNPSQASLLSDKPTSSLYSKRSKQSSHSNPSLGHEKITPGFDKPGKISSPTASQLTLTAKNQQPSSSIVI 340
[Close]
BLAST of SMED30010144 vs. Planmine SMEST
Match: SMESG000038665.1 (SMESG000038665.1)

HSP 1 Score: 599.742 bits (1545), Expect = 0.000e+0
Identity = 302/304 (99.34%), Postives = 302/304 (99.34%), Query Frame = 3 
Query:  141 MNRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQFELESVHTMMGPSIYDPRGTSSASKISAEXXXXXXXXXXXXTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQT 1052
            MNRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQ ELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQ 
Sbjct:    1 MNRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQLELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQV 304
[Close]
BLAST of SMED30010144 vs. Planmine SMEST
Match: SMESG000038665.1 (SMESG000038665.1)

HSP 1 Score: 588.186 bits (1515), Expect = 0.000e+0
Identity = 320/340 (94.12%), Postives = 321/340 (94.41%), Query Frame = 3 
Query:  246 MFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQFELESVHTMMGPSIYDPRGTSSASKISAEXXXXXXXXXXXXTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQTNKNPSQASLLSDKPTXXXXXXXXXXXXXXXXXLGHEKITPGFDKPGKISSPTASQLTLTARNQQPSSSIVI 1265
            MFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQ ELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSK                  TNKNPSQASLLSDKPTSSLYSKRSKQSSHSNPSLGHEKITPGFDKPGKISSPTASQLTLTA+NQQPSSSIVI
Sbjct:    1 MFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQLELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSK------------------TNKNPSQASLLSDKPTSSLYSKRSKQSSHSNPSLGHEKITPGFDKPGKISSPTASQLTLTAKNQQPSSSIVI 322
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BLAST of SMED30010144 vs. Planmine SMEST
Match: SMESG000038665.1 (SMESG000038665.1)

HSP 1 Score: 565.844 bits (1457), Expect = 0.000e+0
Identity = 285/287 (99.30%), Postives = 285/287 (99.30%), Query Frame = 3 
Query:  192 MISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQFELESVHTMMGPSIYDPRGTSSASKISAEXXXXXXXXXXXXTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQT 1052
            MISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQ ELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQ 
Sbjct:    1 MISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLWEVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLSYISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGVNTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNNCVSWGCGKNKMDQQQFSNQLELESVHTMMGPSIYDPRGTSSASKISAERDRSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIPGNQV 287
The following BLAST results are available for this feature:
BLAST of SMED30010144 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pck4.729e-1024.29gene:FBgn0013720 transcript:FBtr0070256[more]
pck4.729e-1024.29gene:FBgn0013720 transcript:FBtr0070255[more]
kune1.247e-821.86gene:FBgn0033032 transcript:FBtr0086037[more]
sinu6.039e-621.08gene:FBgn0010894 transcript:FBtr0077136[more]
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BLAST of SMED30010144 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0R4253.394e-2328.92Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A504YNA14.461e-2329.90Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A4Z2DDK42.934e-2230.98Uncharacterized protein OS=Schistosoma japonicum O... [more]
A0A4Z2DEH43.742e-2230.98Uncharacterized protein OS=Schistosoma japonicum O... [more]
A0A4Z2DDP74.561e-2230.98Uncharacterized protein OS=Schistosoma japonicum O... [more]
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BLAST of SMED30010144 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010144 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038665.10.000e+094.67SMESG000038665.1[more]
SMESG000038665.10.000e+094.41SMESG000038665.1[more]
SMESG000038665.10.000e+099.34SMESG000038665.1[more]
SMESG000038665.10.000e+094.12SMESG000038665.1[more]
SMESG000038665.10.000e+099.30SMESG000038665.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010144 ID=SMED30010144|Name=SMED30010144|organism=Schmidtea mediterranea sexual|type=transcript|length=1958bp
ATTAGTATTTAAATTTTTGAGATTTAAGTATTTTTTGTTTTATTTGTTTA
ATTAGTTTATGTTTGATGAGGAAACTTAAATTATAATTTGAGATGAGTTA
TTTTTAATATTTAATAATTTAATATCAGAATTCACTGATAATGAATCGAC
TTGACAATAGCCCGTTGTTCGTAGCCTTATTTATCTTTACTATGATATCA
TATTTATTTTCCACTCTGGCATTTATTGTATCACAATGGTATTCAATGTT
TCCAGGATCATTTTCAGATTTTCAAAACATTGGCCTTTGGGAAGTTTGCT
TTAATAGTTTCATGAATGAAGAAATATACGATAAACTATTTAGTGATTGT
TTTTATATCAGCTCATCTGATATAAGTGACTTGTATAATTGGATTGTAAC
AAATTGGTTTTTAGCTTGTCAAGTTATTTACACATTAAGTTATATTAGTT
TAATGTTGGCAGCCTTAATATGCACACTAATGTCGCTTGGTTTAATGAAC
CTTAGACATTTTAAAAATGTCATTGTACTTATTTTATTACATTGCATAGC
CTGGGTAACCTGTTTTGCTACATTAATTCTTTTCGGAGTCAACACGAAAA
ACATGTCTGAATACCAGTGGATGCCAAAACCCACTCAATGCACTTTATCA
TGGAGTTATGGATCTGCATCTTTGTCTTTATTAGCTACTATGGTTTCATT
GGGATTGCTTTTCTTCGTCGTAAAATCCAATCGCAACAATTGCGTTAGTT
GGGGTTGTGGAAAAAATAAAATGGATCAACAACAGTTTTCGAATCAATTC
GAACTAGAAAGCGTCCATACGATGATGGGTCCAAGCATTTATGATCCACG
AGGTACCAGTTCTGCTTCGAAAATCAGCGCGGAACGAGATCGATCTGTAA
TTTCCTCTCGAGTCGTTTCAACTCATGCCTCGAAGAATGAAAATCAGCCC
GAAAAATCGCATCAAATTCGGCATCATACTGAGAAGTCATCAAAGTTTAT
GAAAAATAATTCTCGTGCGAATGTACCAAAATATATCCCAGGCAATCAAA
CAAACAAGAATCCATCACAAGCATCACTTCTTAGTGATAAACCGACCTCG
AGTTTATATTCAAAAAGATCCAAACAATCATCACACTCGAATCCATCGTT
GGGACATGAAAAAATAACTCCTGGATTTGATAAACCTGGCAAAATCTCTT
CACCGACGGCCTCTCAACTGACTTTGACCGCTAGAAATCAGCAACCATCA
TCATCGATAGTAATTAAGCTGTGTTCTTGGCTAATCCAACCCGTTGCGAA
GAAAGGATCGTCTTTTTAATTTAATTTTTAGATATAACGGGCAAATATAA
ACCTTAGATATTCAAAAACTGATGGAAATACACTGAATTAAAGAAATATA
ATAAAAACAGAAAGCAAACTGAAATATTTAGTTGAATATAATGTCTTTAC
AAATCCGATACATGTCGCAGATATGTGTCTTAAAGCATCAGATTCTATAT
AATCGAGGGGAATGTAATAGAATTTAATTTGTTTTAACGCTGTGAAATAA
ATGAATTTCGTGTATTCAAATATAATTCATTGCACAATGTTTCAATAAAT
TAACTCAAAAGCTTGAGACATTATTTATTGAATTATTCTAAATGTTTGTT
TCAAATTCAAAGTATGAAGACGCCACTGTTTAATGAATTTTTCATTTATA
CACAGGTTTTGTGGACTATTTGAATAATTATCATCAACTTTTCTATAATT
GTTTATGTGATTTGTGGTCAATGAGTTCGTTCTGTAGAAATAGTTTACAT
AATGTTTATATTATAGTGTTTTATATTATATATTATCAGTTGCTATTTTT
ATTTTATCGTTTTGTTATTAAAATAGATTTCATATTGTCGTTAATAATTT
TTGCTGAAGTGCTCTATGCGAACAATTTTATTACCAAGGAAATAAAATTA
TTAAAACA
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protein sequence of SMED30010144-orf-1

>SMED30010144-orf-1 ID=SMED30010144-orf-1|Name=SMED30010144-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=393bp
MNRLDNSPLFVALFIFTMISYLFSTLAFIVSQWYSMFPGSFSDFQNIGLW
EVCFNSFMNEEIYDKLFSDCFYISSSDISDLYNWIVTNWFLACQVIYTLS
YISLMLAALICTLMSLGLMNLRHFKNVIVLILLHCIAWVTCFATLILFGV
NTKNMSEYQWMPKPTQCTLSWSYGSASLSLLATMVSLGLLFFVVKSNRNN
CVSWGCGKNKMDQQQFSNQFELESVHTMMGPSIYDPRGTSSASKISAERD
RSVISSRVVSTHASKNENQPEKSHQIRHHTEKSSKFMKNNSRANVPKYIP
GNQTNKNPSQASLLSDKPTSSLYSKRSKQSSHSNPSLGHEKITPGFDKPG
KISSPTASQLTLTARNQQPSSSIVIKLCSWLIQPVAKKGSSF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.140.150coord: 7..205
e-value: 3.0E-12
score: 48.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 287..338
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..286
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 236..350
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 236..266
NoneNo IPR availablePANTHERPTHR21284FAMILY NOT NAMEDcoord: 7..198
NoneNo IPR availableCDDcd150747tmA_Opsin5_neuropsincoord: 90..194
e-value: 0.00829386
score: 35.7136
NoneNo IPR availableTMHMMTMhelixcoord: 9..31
NoneNo IPR availableTMHMMTMhelixcoord: 172..194
NoneNo IPR availableTMHMMTMhelixcoord: 130..152
NoneNo IPR availableTMHMMTMhelixcoord: 96..118