Poly [ADP-ribose] polymerase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010139 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Human
Match: TNKS (tankyrase [Source:HGNC Symbol;Acc:HGNC:11941]) HSP 1 Score: 1175.61 bits (3040), Expect = 0.000e+0 Identity = 650/1217 (53.41%), Postives = 821/1217 (67.46%), Query Frame = 2
Query: 377 GANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSN----KTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRT---DGDANTSNNNN--CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQ 3994
GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T ELRE L +E KG L R + KVK+ L + ++INF P + +T +H ++S + KRK + E LLR+GA ++N NK +PL H+AAE + + +L +G+ T+ +RA+ L + C L DP+ + F + N V+ + E T +Y ++LEASKAGD+E V ++ + + VNCRD +GRHSTPLHFAAG+NR+SVV+ LL +GADVHAKDKGGLVPLHNACSYGH +VAELL+++ A+VNV DLWKFTPLHEAAAKGK+EICKLLL++ ADP K+N+DG+TPLDLVKEGD D+ DLL D+ALL+AAKKG +A+VQKL T NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + DG A T L++++++FL+S+ L+ L ++F EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KG+ + G++P + H + T+L+DL P D +YQSVEE+MQS+IR H+D N GGIFNRY +++++++ N+KL +++ R++E+ EEN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P PSQ
Sbjct: 2 GANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLAL----------------EIINFKQPQSHETALHCAVAS--LHPKRKQVTELLLRKGA--NVNEKNKDFMTPL-----------------------------------HVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAA-LAGHLQTCRLLLSYGS-DPSIISLQG-------FTAAQMGNEA------------------VQQILSESTPIRTSDVDY--RLLEASKAGDLETVKQLCSSQN------VNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASLI--SPASTPSCLSAASSIDNLTGPLAELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGTNPYLTFHC--------------------------------VNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEA---PSQ 1081 HSP 2 Score: 309.686 bits (792), Expect = 4.423e-87 Identity = 153/303 (50.50%), Postives = 208/303 (68.65%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKL--VFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELL 1117
L +A K DL+ VK L +VN +D GR STPLHFA+G+ R +VE+L+ GA+V KD+GGL+PLHNACS+GH +V ++L+ G+ N D+W +TPLHEAA KGK ++C +LL+ AD KN DG T DL + +T + + G+ LL+AA+ G + + TP N+NC + GR STPLHLAAGYN + + LLE GA V+A+DKGGL+PLHNA SYGH+++A L++ VNA D W +TPLHEAA KGRT++C+LLLA+GADPT N ++PL+LA D++R LL
Sbjct: 417 LLEASKAGDLETVKQLCS-SQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGD---AALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALL 715 HSP 3 Score: 305.449 bits (781), Expect = 1.534e-85 Identity = 231/648 (35.65%), Postives = 333/648 (51.39%), Query Frame = 2
Query: 836 MGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEE-INIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSE--KEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPK-FEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADV 2767
MGA VHA+D GGL+PLHNACS+GH EV LL +GA NA D W YTPLHEAA KG+ +VC +LL +GADP N KS L+L A + +L E+K L R G K+ +L L + NC ++ T +H N V +++ LL+ GA ++ +K G PLH A + ++ +LL+++ +N L T LH AA N +C +L+++G++ T+ G A + +L E L+ K + AA +D K+ T ++ N K+P+ E + V S ++ KQV E ++ K +V E N D TPLH AA V++VL ++GA ++A D G LH A GH + LL+ Y ++ ++ L FT AA G + ++L + TP ++ D D LLEA+K G + V++L +S N+NCRD +GR+STPLH A+GYN + + E+LL GADV+A+DKGGL+PLHNA SYGH ++A LL+++ A+VN AD W +TPLHEAA K + ++C LLL HGAD KN +G +PLDL D
Sbjct: 1 MGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDL-ADPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPL----NVNCHASDGRK-----------STPLHLAAGYNRV-----RIVQLLLQHGA--DVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFT---PLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLALEII--------NFKQPQSHETALHCAVASLHPKR----KQVTELLLR-----KGANVN------EKNKDFM--------------TPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFT----AAQMGNEAVQQILSES------------TP---IRTSDVDY--------RLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDT 558 HSP 4 Score: 243.047 bits (619), Expect = 5.930e-65 Identity = 211/742 (28.44%), Postives = 337/742 (45.42%), Query Frame = 2
Query: 110 NPNEYCTDIQDICDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGY---NRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELL------YFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGD 2305
+PN TD + D +AD S + ++++ L +A ++ + + + LL +VN + GRKSTPLH A+G+ R +V+ L+ GA+V KD+GGL+PLHNACS+GH +V ++L+ G+ NA D+W +TPLHEAA K + +VC +LL AD L N G +A D+A + T E+K LL+AAR + L +N + T LH A R ++ +LLL GA V+ K+K + PLH A H +V + L + GA +NA D T LH AA G + C LLL+YG+DP+ ++ + ++ + ++++L + L + G+ VK+ L ++ + NC + + T +H N V +++E+LL GA ++ +K G PLH A + ++ +LLVR+ +N+ L T LH AA IC +L+ +G++ T G L + D ++ L AA + + K KL T + ++ +G + P+ N+ +V EY +LE+ +D VN +D G PLH AA + + + +L++ V+A DK PLH A G ++ L LL + ADP +N++G TPLDL D
Sbjct: 70 DPNIRNTDGKSALD-LADPSAKAVLTGEYKKD--ELLEAARSGNEEKLMALL-TPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLALEIINFKQPQSHE-TALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQM-GNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQ----LCSSQNVNCRDLEGRH-----------STPLHFAAGYNRV-----SVVEYLLHHGA--DVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFT---PLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGD----TDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDT-----QGRNSTPLHLAAG---YNNLEVAEY------------------LLEHGAD-----VNAQDKGGL--IPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCAL---------------------------------LLAHGADPTMKNQEGQTPLDLATADD 710
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Human
Match: TNKS (tankyrase [Source:HGNC Symbol;Acc:HGNC:11941]) HSP 1 Score: 1122.07 bits (2901), Expect = 0.000e+0 Identity = 637/1272 (50.08%), Postives = 834/1272 (65.57%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRT---DGDANTSNNNN--CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQ 3994
R L +AC+N D+ VK L+D +VN+KD GRKS+PLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T ELRE L +E KG L R + KVK+ L + ++INF P + +T +H ++S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L++YGS+ +I + QG+ A+Q+ + + + LSE T SD + +L +K + V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLDLVKEGD D+ DLL D+ALL+AAKKG +A+VQKL T NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + DG A T L++++++FL+S+ L+ L ++F EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KG+ + G++P + H + T+L+DL P D +YQSVEE+MQS+IR H+D N GGIFNRY +++++++ N+KL +++ R++E+ EEN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P PSQ
Sbjct: 184 RELLEACRNGDVSRVKRLVDAA-NVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLAL----------------EIINFKQPQSHETALHCAVAS--LHPKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTL----GQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEA-VQQILSES-----TPIRTSDVD-------YRLLEASKAGDLET------------VKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASLI--SPASTPSCLSAASSIDNLTGPLAELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGTNPYLTFHC--------------------------------VNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEA---PSQ 1318 HSP 2 Score: 251.906 bits (642), Expect = 2.784e-67 Identity = 179/518 (34.56%), Postives = 263/518 (50.77%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + DL VK L + +N K + T LH A RK++ E L+ +GANV+ K++ + PLH A H DV++VL G+ NA D T LH AA+ G C +LL +D ++ + G TA + + +++ + LLEA++ G ET+ + + NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +V Q L + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L T D+ L + + C +V
Sbjct: 485 TYEFKGHSLLQAAREADLAKVKKTLALEI-INFKQPQSHE-TALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCTPENINCRDTQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVV 972
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Human
Match: TNKS (tankyrase [Source:HGNC Symbol;Acc:HGNC:11941]) HSP 1 Score: 1104.35 bits (2855), Expect = 0.000e+0 Identity = 629/1272 (49.45%), Postives = 829/1272 (65.17%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRT---DGDANTSNNNN--CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQ 3994
R L +AC+N D+ VK L+D +VN+KD GRKS+PLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TG+ K E ++ R+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T ELRE L +E KG L R + KVK+ L + ++INF P + +T +H ++S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L++YGS+ +I + QG+ A+Q+ + + + LSE T SD + +L +K + V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLDLVKEGD D+ DLL D+ALL+AAKKG +A+VQKL T NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + DG A T L++++++FL+S+ L+ L ++F EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KG+ + G++P + H + T+L+DL P D +YQSVEE+MQS+IR H+D N GGIFNRY +++++++ N+KL +++ R++E+ EEN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P PSQ
Sbjct: 184 RELLEACRNGDVSRVKRLVDAA-NVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGQCKSSEHVKFYRSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLAL----------------EIINFKQPQSHETALHCAVAS--LHPKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTL----GQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEA-VQQILSES-----TPIRTSDVDY-------RLLEASKAGDLET------------VKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASLI--SPASTPSCLSAASSIDNLTGPLAELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGTNPYLTFHC--------------------------------VNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEA---PSQ 1318 HSP 2 Score: 251.906 bits (642), Expect = 2.760e-67 Identity = 179/518 (34.56%), Postives = 263/518 (50.77%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + DL VK L + +N K + T LH A RK++ E L+ +GANV+ K++ + PLH A H DV++VL G+ NA D T LH AA+ G C +LL +D ++ + G TA + + +++ + LLEA++ G ET+ + + NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +V Q L + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L T D+ L + + C +V
Sbjct: 485 TYEFKGHSLLQAAREADLAKVKKTLALEI-INFKQPQSHE-TALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCTPENINCRDTQGRN-----------STPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVV 972
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Human
Match: TNKS (tankyrase [Source:HGNC Symbol;Acc:HGNC:11941]) HSP 1 Score: 1104.35 bits (2855), Expect = 0.000e+0 Identity = 629/1272 (49.45%), Postives = 829/1272 (65.17%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRT---DGDANTSNNNN--CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQ 3994
R L +AC+N D+ VK L+D +VN+KD GRKS+PLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TG+ K E ++ R+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T ELRE L +E KG L R + KVK+ L + ++INF P + +T +H ++S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L++YGS+ +I + QG+ A+Q+ + + + LSE T SD + +L +K + V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLDLVKEGD D+ DLL D+ALL+AAKKG +A+VQKL T NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + DG A T L++++++FL+S+ L+ L ++F EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KG+ + G++P + H + T+L+DL P D +YQSVEE+MQS+IR H+D N GGIFNRY +++++++ N+KL +++ R++E+ EEN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P PSQ
Sbjct: 184 RELLEACRNGDVSRVKRLVDAA-NVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGQCKSSEHVKFYRSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLAL----------------EIINFKQPQSHETALHCAVAS--LHPKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTL----GQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEA-VQQILSES-----TPIRTSDVDY-------RLLEASKAGDLET------------VKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASLI--SPASTPSCLSAASSIDNLTGPLAELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGTNPYLTFHC--------------------------------VNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEA---PSQ 1318 HSP 2 Score: 251.906 bits (642), Expect = 2.760e-67 Identity = 179/518 (34.56%), Postives = 263/518 (50.77%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + DL VK L + +N K + T LH A RK++ E L+ +GANV+ K++ + PLH A H DV++VL G+ NA D T LH AA+ G C +LL +D ++ + G TA + + +++ + LLEA++ G ET+ + + NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +V Q L + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L T D+ L + + C +V
Sbjct: 485 TYEFKGHSLLQAAREADLAKVKKTLALEI-INFKQPQSHE-TALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCTPENINCRDTQGRN-----------STPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVV 972
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Human
Match: TNKS2 (tankyrase 2 [Source:HGNC Symbol;Acc:HGNC:15677]) HSP 1 Score: 756.133 bits (1951), Expect = 0.000e+0 Identity = 431/863 (49.94%), Postives = 568/863 (65.82%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMK---EKTTNDKQVEEYCMQILE-ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAI 2791
LF+AC+N D++ VK L+ VNS+D GRKSTPLHFA+GFGRK++VE+L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ A+ ++N+DG TA DLA D + K V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL+ GA VHAKDKG LVPLHNACSYGH EV + L++ GA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E L +E KG L R + ++K+ L + +++NF HP T +T +H +S KRK + E LLR+GA ++N K+ +PLH+A + D+++++V++ E ++ L+ + G+ +LH AA C +L++YG + I + QG+ A Q+ + ++ + L E L N++ D+ E A VE+ K ++ N + +E L A+ V +V +L++ +D V+ +D G PLH A + V ++L+++GA V+ D PLH A + G ++ +L LLQ+ ADP K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +A+V+KL + N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL SA DV LL A+
Sbjct: 28 LFEACRNGDVERVKRLV-TPEKVNSRDTAGRKSTPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLA-DPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKHLSL----------------EMVNFKHPQTHETALHCAAASP--YPKRKQICELLLRKGA--NINEKTKEFLTPLHVASEKAHNDVVEVVVKH-EAKVNALDNL-GQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNE-NVQQLLQEGISLG----------------------NSEADRQ-----LLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKL---------------------------------LLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLTAAM 798 HSP 2 Score: 367.466 bits (942), Expect = 1.711e-106 Identity = 182/342 (53.22%), Postives = 246/342 (71.93%), Query Frame = 2
Query: 2951 TDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
T+G ++ ++ S+ +F+R++ L+ L+++F +EQ+T+D+L+EMGH ELKE+G+ AYGHRHK++KG+ + G +P + ++++ G G T+L+DL P D ++QSVEE+MQS++R H+D + GGIFNRY ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 860 TEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERL---------ISGQQGLNPYLTLNTS--------GSG------------------------TILIDLSPDDKEFQSVEEEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRP 1160 HSP 3 Score: 260.766 bits (665), Expect = 1.832e-70 Identity = 179/499 (35.87%), Postives = 268/499 (53.71%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTG-----EYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLS 1654
++E +L A + D+ +K L + VN K + T LH A+ RK++ E L+ +GAN++ K + L PLH A H DVV+V++ + NA D T LH AA G C +LL D N+ + G TA + +N +L+ G ++LLEAA+ G ET+ + T +VNC +GR+STPLH AAGYNR +V+ LL+ GA VHAKDKGGLVPLHNACSYGH EVA+ L++ GA+VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +VK+ L + ++ NC + + T +H NN+ + E+LL+ GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L + D+ L+
Sbjct: 327 AYEFKGHSLLQAAREADVTRIKKHLSLEM-VNFK-HPQTHETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVVKHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGISLGNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGD---AALLDAAKKGCLARVKK----LSSPDNVNCRDTQGRHS-----------TPLHLAAGYNNL-----EVAEYLLQHGA--DVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLT 795 HSP 4 Score: 232.646 bits (592), Expect = 1.618e-61 Identity = 178/561 (31.73%), Postives = 275/561 (49.02%), Query Frame = 2
Query: 1142 LFNYCRLGEYNKVKRMLQMLG-NTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-----NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGD-QDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGY---NNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAIS 2794
LF CR G+ +VKR++ N+ D+ K ++F F RK+++E+LL+ GA ++ + G PLH A + +++ LL+R N N + LH AA +C +L+ +G+ TI G T +DL + P+ K+ L K D ++LE++++G+ E + +L + VNC +DGR STPLH AAG+NR+ +V++LL++GADVHAKDKG LVPLHNACSYGH +V ELL+++ A VN DLW+FTPLHEAA+K + E+C LLL Y ADP N + +DL ++ +LL+AA++ + +++K ++ +N + Q + T LH A+ +I E LL +GA++N + K L PLH AS H D+ +++K+ A VN+ D G T LH AA Q C LLL++G D + + +G + L + + +V+ LL+E IS
Sbjct: 28 LFEACRNGDVERVKRLVTPEKVNSRDTAGR----KSTPLHFAAGFG-----------------RKDVVEYLLQNGA--NVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNY-------TPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDG------RTALDLAD----------------------PSAKAVLTGEYKKD------------------------------------ELLESARSGNEEKMMALL------TPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKHLSLEMVNFKHPQ-THETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVVKHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGN-ENVQQLLQEGIS 486
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 252.292 bits (643), Expect = 1.530e-67 Identity = 238/877 (27.14%), Postives = 381/877 (43.44%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFT--------GEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVT--GKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVEST------MKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLC 2752
E E + A + DL+ V LL G D+N+ + G S LH AS G E+V LI R A V G LH A G + +V +L+ G++ N + V +TPL+ AA + +V LL+ A+ L DG T +A V G+ + L AA+ + T T+L N + TPLH+AA Y + QLLLE GA V+ + + + PLH A +G +A LL RGA++++ TPLH AA G +V LL+ GA + + +PL +AA D R LLY H+ V D +++ T +H +V + + LL R A + LN G +PLH+A + +++LL++ +E T G LH+AA + + +I L+ G+N + T +G L A ++ +V L N ++E + IA + +T ++ ++ + + A+K G E+ +L++N+D K++L + TPLH A+ + + VV++LLE G V + K + PLH A Y + KVA LL++ A+ +TPLH AA K + EI LLQ+ ADP+ +++ G TPL L +EG +++ LL + ++ + G T +HL + +++ +A+ L GA++N++ G PLH A +G +M L++ A+V + YTPLH+AAQ+ LL +GA + GQ+PL +
Sbjct: 27 EPGRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKD-DTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLY--HRAPV------------------------DDVTVDYL---------------TPLHVAAHCGHV-----RVAKLLLDRSADPNSRALN--GFTPLHIACKKNRIKVVELLLKYRA----AIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHL--------------------AARANQTDVV----RVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD-KTLLTK------KGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL--------------------IENGSDVGAKANNGL--TAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 2 Score: 199.134 bits (505), Expect = 4.047e-51 Identity = 208/796 (26.13%), Postives = 327/796 (41.08%), Query Frame = 2
Query: 527 AAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAA-RNG----------LEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE---RGAMV-----------------------NAPDLWQ---YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILEN--NSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLV---TSVNINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
AA G + + LL+A D+N N++GL + LA+ V K+Q ++AA R G + I+TIL N + TPL++AA N +V+ LL+ GA + G PL A GH V LLE +G + + PD+ +TPLH AA G V LLL GA+ + HN SPL +A + G N +L G DS + + T +H S + +++ L+ +GA ++ K G +PLH+A +VD + L+ + +++ +D L LH+AA + +L++ ++ G+ + AC + K+V E +K + + E + A+ G + +V +L+ N D+++V R TPLH AA N+ VV+VL+ NGA V A+ + PLH A G+ + LL+Q AN N ++PLH AA +G+ E+ +LL +NAD K G TPL L K G + V+ L+ T V+I +G+N TPLH+A+ YNN ++A LLE GA A K G PLH A+ ++A+ LL++ A+ N+ + G+TPLH +AQ+ ++ LL+ +G+D K G + + LC+ D
Sbjct: 40 AARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVA---------------------TKWGRTNMANLLLSR-GAIIDSRTKDLL---------------TPLHCAARSGH-----DQVVDLLVVQGA--PISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL------TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHI--------------------ACKKNRIKVV-------------------------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV---------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLAS--------------------KYGNLEVVRLLLERGTPVDI-----EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQED 700 HSP 3 Score: 148.288 bits (373), Expect = 1.851e-35 Identity = 134/460 (29.13%), Postives = 203/460 (44.13%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELREL--LYFE---------HKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYIL 1561
L ACK N ++VV+ LL + + G TPLH A+ G +V +L+ +GAN ++ G PLH A DVV+VLI G+ +A+ TPLH A+ G D+ I+LLQA A+ N D + +A A+ G EE + IL N + + TPLHLA+ Y +V+LLLE G V + K + PLH A Y + +VA LLE GA A YTPLH AA K + E+ S LL + ADP + +PL L+A + +E+ L E + G + C ++ V ++L G +S + G T +H + N+++FL+ GA + + +PLH A + + ++ L+ N + + TG+ L +A L S+ L
Sbjct: 396 LHIACKKNRIKVVELLLKYRAAIEATTESGL--TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIA---------------------AKEGQEE-VAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTN---------------AGYTPLHVACHFGQL-----NMVKFLVENGA--DVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNE--QTATGQTPLSIAQRLGYVSVVETL 807 HSP 4 Score: 135.961 bits (341), Expect = 1.180e-31 Identity = 105/313 (33.55%), Postives = 160/313 (51.12%), Query Frame = 2
Query: 1850 EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEG-DQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNI------NCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
E L A++AGD+E V +L +DI N + +G +S LH A+ VV+ L++ A V A + G LH A G + + +L++ ANVNV + FTPL+ AA + E+ K LL++ A+ +DG TPL + +++G D+ V LL +DS IA + T+ + N T TPLH+A+ Y + + + LLE+GA+VN Q + + PLH A+ +G +MA LLL A ++S K TPLH AA+ Q+ LL+ GA K G +PL + + D
Sbjct: 32 EGSASFLRAARAGDLEKVLELLRAGTDI-----NTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGD 337 HSP 5 Score: 115.161 bits (287), Expect = 2.290e-25 Identity = 93/297 (31.31%), Postives = 127/297 (42.76%), Query Frame = 2
Query: 188 ASFEENCRTLFDACKNNDLQVVKCLLDIGYDVN--SKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQELLEAARNGLEET----------IMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLL 1027
A+ +N L A K +V LLD D +K F TPLH AS +G E+V L+ RG V I+ + + PLH A + + V +L+ G+ A YTPLH AA K + ++ LLQ KAD N K+ G T L+ K L+ G ++ A NGL + IL ++ TPLH+A + + +V+ L+E GA V K + PLH A GH + LLE GA N TPL A G V L
Sbjct: 519 ATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGF----TPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGS----DVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 252.292 bits (643), Expect = 1.530e-67 Identity = 238/877 (27.14%), Postives = 381/877 (43.44%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFT--------GEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVT--GKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVEST------MKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLC 2752
E E + A + DL+ V LL G D+N+ + G S LH AS G E+V LI R A V G LH A G + +V +L+ G++ N + V +TPL+ AA + +V LL+ A+ L DG T +A V G+ + L AA+ + T T+L N + TPLH+AA Y + QLLLE GA V+ + + + PLH A +G +A LL RGA++++ TPLH AA G +V LL+ GA + + +PL +AA D R LLY H+ V D +++ T +H +V + + LL R A + LN G +PLH+A + +++LL++ +E T G LH+AA + + +I L+ G+N + T +G L A ++ +V L N ++E + IA + +T ++ ++ + + A+K G E+ +L++N+D K++L + TPLH A+ + + VV++LLE G V + K + PLH A Y + KVA LL++ A+ +TPLH AA K + EI LLQ+ ADP+ +++ G TPL L +EG +++ LL + ++ + G T +HL + +++ +A+ L GA++N++ G PLH A +G +M L++ A+V + YTPLH+AAQ+ LL +GA + GQ+PL +
Sbjct: 27 EPGRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKD-DTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLY--HRAPV------------------------DDVTVDYL---------------TPLHVAAHCGHV-----RVAKLLLDRSADPNSRALN--GFTPLHIACKKNRIKVVELLLKYRA----AIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHL--------------------AARANQTDVV----RVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD-KTLLTK------KGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL--------------------IENGSDVGAKANNGL--TAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 2 Score: 199.134 bits (505), Expect = 4.047e-51 Identity = 208/796 (26.13%), Postives = 327/796 (41.08%), Query Frame = 2
Query: 527 AAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAA-RNG----------LEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE---RGAMV-----------------------NAPDLWQ---YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILEN--NSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLV---TSVNINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
AA G + + LL+A D+N N++GL + LA+ V K+Q ++AA R G + I+TIL N + TPL++AA N +V+ LL+ GA + G PL A GH V LLE +G + + PD+ +TPLH AA G V LLL GA+ + HN SPL +A + G N +L G DS + + T +H S + +++ L+ +GA ++ K G +PLH+A +VD + L+ + +++ +D L LH+AA + +L++ ++ G+ + AC + K+V E +K + + E + A+ G + +V +L+ N D+++V R TPLH AA N+ VV+VL+ NGA V A+ + PLH A G+ + LL+Q AN N ++PLH AA +G+ E+ +LL +NAD K G TPL L K G + V+ L+ T V+I +G+N TPLH+A+ YNN ++A LLE GA A K G PLH A+ ++A+ LL++ A+ N+ + G+TPLH +AQ+ ++ LL+ +G+D K G + + LC+ D
Sbjct: 40 AARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVA---------------------TKWGRTNMANLLLSR-GAIIDSRTKDLL---------------TPLHCAARSGH-----DQVVDLLVVQGA--PISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL------TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHI--------------------ACKKNRIKVV-------------------------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV---------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLAS--------------------KYGNLEVVRLLLERGTPVDI-----EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQED 700 HSP 3 Score: 148.288 bits (373), Expect = 1.851e-35 Identity = 134/460 (29.13%), Postives = 203/460 (44.13%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELREL--LYFE---------HKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYIL 1561
L ACK N ++VV+ LL + + G TPLH A+ G +V +L+ +GAN ++ G PLH A DVV+VLI G+ +A+ TPLH A+ G D+ I+LLQA A+ N D + +A A+ G EE + IL N + + TPLHLA+ Y +V+LLLE G V + K + PLH A Y + +VA LLE GA A YTPLH AA K + E+ S LL + ADP + +PL L+A + +E+ L E + G + C ++ V ++L G +S + G T +H + N+++FL+ GA + + +PLH A + + ++ L+ N + + TG+ L +A L S+ L
Sbjct: 396 LHIACKKNRIKVVELLLKYRAAIEATTESGL--TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIA---------------------AKEGQEE-VAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTN---------------AGYTPLHVACHFGQL-----NMVKFLVENGA--DVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNE--QTATGQTPLSIAQRLGYVSVVETL 807 HSP 4 Score: 135.961 bits (341), Expect = 1.180e-31 Identity = 105/313 (33.55%), Postives = 160/313 (51.12%), Query Frame = 2
Query: 1850 EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEG-DQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNI------NCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
E L A++AGD+E V +L +DI N + +G +S LH A+ VV+ L++ A V A + G LH A G + + +L++ ANVNV + FTPL+ AA + E+ K LL++ A+ +DG TPL + +++G D+ V LL +DS IA + T+ + N T TPLH+A+ Y + + + LLE+GA+VN Q + + PLH A+ +G +MA LLL A ++S K TPLH AA+ Q+ LL+ GA K G +PL + + D
Sbjct: 32 EGSASFLRAARAGDLEKVLELLRAGTDI-----NTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGD 337 HSP 5 Score: 115.161 bits (287), Expect = 2.290e-25 Identity = 93/297 (31.31%), Postives = 127/297 (42.76%), Query Frame = 2
Query: 188 ASFEENCRTLFDACKNNDLQVVKCLLDIGYDVN--SKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQELLEAARNGLEET----------IMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLL 1027
A+ +N L A K +V LLD D +K F TPLH AS +G E+V L+ RG V I+ + + PLH A + + V +L+ G+ A YTPLH AA K + ++ LLQ KAD N K+ G T L+ K L+ G ++ A NGL + IL ++ TPLH+A + + +V+ L+E GA V K + PLH A GH + LLE GA N TPL A G V L
Sbjct: 519 ATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGF----TPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGS----DVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 247.669 bits (631), Expect = 9.406e-66 Identity = 236/875 (26.97%), Postives = 382/875 (43.66%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLAT----DNNTKLVFTGEYKKQELLEA---ARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVT--GKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVEST------MKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDL 2749
E E + A + DL+ V LL G D+N+ + G S LH AS G E+V LI R A V G LH A G + +V +L+ G++ N + V +TPL+ AA + +V LL+ A+ L DG T +A D ++ + K + L A A + T T+L N + TPLH+AA Y + QLLLE GA V+ + + + PLH A +G +A LL RGA++++ TPLH AA G +V LL+ GA + + +PL +AA D R LLY H+ V ++++ P +H +V + + LL R A + LN G +PLH+A + +++LL++ +E T G LH+AA + + +I L+ G+N + T +G L A ++ +V L N ++E + IA + +T ++ ++ + + A+K G E+ +L++N+D K++L + TPLH A+ + + VV++LLE G V + K + PLH A Y + KVA LL++ A+ +TPLH AA K + EI LLQ+ ADP+ +++ G TPL L +EG +++ G + + ++ + G T +HL + +++ +A+ L GA++N++ G PLH A +G +M L++ A+V + YTPLH+AAQ+ LL +GA + GQ+PL +
Sbjct: 27 EPGRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLY--HRAPV---------------------------------DDVTVDYLTP------LHVAAHCGHV-----RVAKLLLDRSADPNSRALN--GFTPLHIACKKNRIKVVELLLKYRA----AIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHL--------------------AARANQTDVVRV----LIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD-KTLLTK------KGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEI---------------SGLLIE-----NGSDVGAKANNGL--TAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSI 794 HSP 2 Score: 194.897 bits (494), Expect = 1.634e-49 Identity = 208/796 (26.13%), Postives = 324/796 (40.70%), Query Frame = 2
Query: 527 AAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAA-RNGL----------EETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAM--------------------------VNAPDLWQ---YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILEN--NSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLV---TSVNINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
AA G + + LL+A D+N N++GL + LA+ V K+Q ++AA R G + I+TIL N + TPL++AA N +V+ LL+ GA + G PL A GH V LLE + + PD+ +TPLH AA G V LLL GA+ + HN SPL +A + G N +L G DS + + T +H S + +++ L+ +GA S K G +PLH+A +VD + L+ + +++ +D L LH+AA + +L++ ++ G+ + AC + K+V E +K + + E + A+ G + +V +L+ N D+++V R TPLH AA N+ VV+VL+ NGA V A+ + PLH A G+ + LL+Q AN N ++PLH AA +G+ E+ +LL +NAD K G TPL L K G + V+ L+ T V+I +G+N TPLH+A+ YNN ++A LLE GA A K G PLH A+ ++A+ LL++ A+ N+ + G+TPLH +AQ+ ++ LL+ +G+D K G + + LC+ D
Sbjct: 40 AARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVAT---------------------KWGRTNMANLLLSR-GAIIDSRTKDLL---------------TPLHCAARSGH-----DQVVDLLVVQGAPISAKT--KNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL------TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHI--------------------ACKKNRIKVV-------------------------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV---------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLAS--------------------KYGNLEVVRLLLERGTPVDI-----EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQED 700 HSP 3 Score: 133.65 bits (335), Expect = 8.285e-31 Identity = 104/307 (33.88%), Postives = 159/307 (51.79%), Query Frame = 2
Query: 1868 LEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEG-DQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNI------NCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
L A++AGD+E V +L +DI N + +G +S LH A+ VV+ L++ A V A + G LH A G + + +L++ ANVNV + FTPL+ AA + E+ K LL++ A+ +DG TPL + +++G D+ V LL +DS IA + T+ + N T TPLH+A+ Y + + + LLE+GA+VN Q + + PLH A+ +G +MA LLL A ++S K TPLH AA+ Q+ LL+ GA K G +PL + + D
Sbjct: 38 LRAARAGDLEKVLELLRAGTDI-----NTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGD 337 HSP 4 Score: 112.849 bits (281), Expect = 1.627e-24 Identity = 93/299 (31.10%), Postives = 128/299 (42.81%), Query Frame = 2
Query: 182 TEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVN--SKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQELLEAARNGLEET----------IMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLL 1027
+ A+ +N L A K +V LLD D +K F TPLH AS +G E+V L+ RG V I+ + + PLH A + + V +L+ G+ A YTPLH AA K + ++ LLQ KAD N K+ G T L+ K L+ G ++ A NGL + IL ++ TPLH+A + + +V+ L+E GA V K + PLH A GH + LLE GA N TPL A G V L
Sbjct: 517 SNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGF----TPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENG----SDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 247.669 bits (631), Expect = 9.406e-66 Identity = 236/875 (26.97%), Postives = 382/875 (43.66%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLAT----DNNTKLVFTGEYKKQELLEA---ARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVT--GKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVEST------MKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDL 2749
E E + A + DL+ V LL G D+N+ + G S LH AS G E+V LI R A V G LH A G + +V +L+ G++ N + V +TPL+ AA + +V LL+ A+ L DG T +A D ++ + K + L A A + T T+L N + TPLH+AA Y + QLLLE GA V+ + + + PLH A +G +A LL RGA++++ TPLH AA G +V LL+ GA + + +PL +AA D R LLY H+ V ++++ P +H +V + + LL R A + LN G +PLH+A + +++LL++ +E T G LH+AA + + +I L+ G+N + T +G L A ++ +V L N ++E + IA + +T ++ ++ + + A+K G E+ +L++N+D K++L + TPLH A+ + + VV++LLE G V + K + PLH A Y + KVA LL++ A+ +TPLH AA K + EI LLQ+ ADP+ +++ G TPL L +EG +++ G + + ++ + G T +HL + +++ +A+ L GA++N++ G PLH A +G +M L++ A+V + YTPLH+AAQ+ LL +GA + GQ+PL +
Sbjct: 27 EPGRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLY--HRAPV---------------------------------DDVTVDYLTP------LHVAAHCGHV-----RVAKLLLDRSADPNSRALN--GFTPLHIACKKNRIKVVELLLKYRA----AIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHL--------------------AARANQTDVVRV----LIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD-KTLLTK------KGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEI---------------SGLLIE-----NGSDVGAKANNGL--TAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSI 794 HSP 2 Score: 194.897 bits (494), Expect = 1.634e-49 Identity = 208/796 (26.13%), Postives = 324/796 (40.70%), Query Frame = 2
Query: 527 AAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAA-RNGL----------EETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAM--------------------------VNAPDLWQ---YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILEN--NSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLV---TSVNINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
AA G + + LL+A D+N N++GL + LA+ V K+Q ++AA R G + I+TIL N + TPL++AA N +V+ LL+ GA + G PL A GH V LLE + + PD+ +TPLH AA G V LLL GA+ + HN SPL +A + G N +L G DS + + T +H S + +++ L+ +GA S K G +PLH+A +VD + L+ + +++ +D L LH+AA + +L++ ++ G+ + AC + K+V E +K + + E + A+ G + +V +L+ N D+++V R TPLH AA N+ VV+VL+ NGA V A+ + PLH A G+ + LL+Q AN N ++PLH AA +G+ E+ +LL +NAD K G TPL L K G + V+ L+ T V+I +G+N TPLH+A+ YNN ++A LLE GA A K G PLH A+ ++A+ LL++ A+ N+ + G+TPLH +AQ+ ++ LL+ +G+D K G + + LC+ D
Sbjct: 40 AARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVAT---------------------KWGRTNMANLLLSR-GAIIDSRTKDLL---------------TPLHCAARSGH-----DQVVDLLVVQGAPISAKT--KNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL------TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHI--------------------ACKKNRIKVV-------------------------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV---------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLAS--------------------KYGNLEVVRLLLERGTPVDI-----EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQED 700 HSP 3 Score: 133.65 bits (335), Expect = 8.285e-31 Identity = 104/307 (33.88%), Postives = 159/307 (51.79%), Query Frame = 2
Query: 1868 LEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEG-DQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNI------NCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
L A++AGD+E V +L +DI N + +G +S LH A+ VV+ L++ A V A + G LH A G + + +L++ ANVNV + FTPL+ AA + E+ K LL++ A+ +DG TPL + +++G D+ V LL +DS IA + T+ + N T TPLH+A+ Y + + + LLE+GA+VN Q + + PLH A+ +G +MA LLL A ++S K TPLH AA+ Q+ LL+ GA K G +PL + + D
Sbjct: 38 LRAARAGDLEKVLELLRAGTDI-----NTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGD 337 HSP 4 Score: 112.849 bits (281), Expect = 1.627e-24 Identity = 93/299 (31.10%), Postives = 128/299 (42.81%), Query Frame = 2
Query: 182 TEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVN--SKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQELLEAARNGLEET----------IMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLL 1027
+ A+ +N L A K +V LLD D +K F TPLH AS +G E+V L+ RG V I+ + + PLH A + + V +L+ G+ A YTPLH AA K + ++ LLQ KAD N K+ G T L+ K L+ G ++ A NGL + IL ++ TPLH+A + + +V+ L+E GA V K + PLH A GH + LLE GA N TPL A G V L
Sbjct: 517 SNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGF----TPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENG----SDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 242.276 bits (617), Expect = 2.038e-64 Identity = 239/862 (27.73%), Postives = 360/862 (41.76%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFT--------GEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFV----------LFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFID-----KQQLINFVHPF------------TGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHS-TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTS-VNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPL 2644
E E + A + DL+ V LL G D+N+ + G S LH AS G E+V LI R A V G LH A G + +V +L+ G++ N + V +TPL+ AA + +V LL+ A+ L DG T +A V G+ + L AA+ + T T+L N + TPLH+AA Y + QLLLE GA V+ + + + PLH A +G +A LL RGA++++ TPLH AA G +V LL+ GA + + +PL +AA D R LLY H+ V L G K +L + N + F K+ I V +G T +H + N++ +LL++GA + + +G +PLHLA D++++L+RN +++ E T LH+A+ L ++ I +L+ G+N T Y S L ++ KE + A + D N K FT P+ K G++E+V +LE + + D +G++ TPLH AA +N V +LLENGA A K G PLH A ++A L+Q+ A+ N FTPLH +A +G EI LL++ +D + +G T + L + D + Q L + IN + G TPLH+A + L + +FL+E GADV + + PLH A+ GH + LL+ A+ N G TPL
Sbjct: 27 EPGRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKD-DTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLY--HRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAI-----NIVIYLLQQGANPDVETV--RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQT---PLHIASRLGNTDIVILLLQAGANSNATTRDNY--SPL--------HIAAKEGQEEVAGILLDHNADKTLLTKKGFT--------------------PLHLASK---------------------YGNLEVVRLLLERGTPV--------DIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQED--------------------HVPVAQILYNNGAEINSKTNAG--YTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPL 792 HSP 2 Score: 199.134 bits (505), Expect = 4.844e-51 Identity = 208/796 (26.13%), Postives = 327/796 (41.08%), Query Frame = 2
Query: 527 AAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAA-RNG----------LEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE---RGAMV-----------------------NAPDLWQ---YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILEN--NSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLV---TSVNINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
AA G + + LL+A D+N N++GL + LA+ V K+Q ++AA R G + I+TIL N + TPL++AA N +V+ LL+ GA + G PL A GH V LLE +G + + PD+ +TPLH AA G V LLL GA+ + HN SPL +A + G N +L G DS + + T +H S + +++ L+ +GA ++ K G +PLH+A +VD + L+ + +++ +D L LH+AA + +L++ ++ G+ + AC + K+V E +K + + E + A+ G + +V +L+ N D+++V R TPLH AA N+ VV+VL+ NGA V A+ + PLH A G+ + LL+Q AN N ++PLH AA +G+ E+ +LL +NAD K G TPL L K G + V+ L+ T V+I +G+N TPLH+A+ YNN ++A LLE GA A K G PLH A+ ++A+ LL++ A+ N+ + G+TPLH +AQ+ ++ LL+ +G+D K G + + LC+ D
Sbjct: 40 AARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVA---------------------TKWGRTNMANLLLSR-GAIIDSRTKDLL---------------TPLHCAARSGH-----DQVVDLLVVQGA--PISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL------TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHI--------------------ACKKNRIKVV-------------------------------ELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV---------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLAS--------------------KYGNLEVVRLLLERGTPVDI-----EGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQED 700 HSP 3 Score: 187.193 bits (474), Expect = 2.081e-47 Identity = 210/834 (25.18%), Postives = 356/834 (42.69%), Query Frame = 2
Query: 329 ASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA---ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDT-----LIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-RNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGY---RASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSK-------LFTNNKTDKWNSKEPKFEGEIAPPVEST------MKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLC 2752
A+ G E V L+ G +++ + GL LH A GH++VV+ LI + +A T LH A++ G++ + +L++ A+VN+++ +G T +A N E ++ L N S TPL +A R+V +LLE + K K L LH A A LL+ + +TPLH AA G V LLL GA+ + HN SPL +A + LL +G ++ ++ K +L L S +D L+ P + T +H ++V + R L R + V +PLH+A +V + +LL+ R+ + N L G T LH+A + N + +L+ Y + T G + I++ +L + + +P + + ++ L N ++E + IA + +T ++ ++ + + A+K G E+ +L++N+D K++L + TPLH A+ + + VV++LLE G V + K + PLH A Y + KVA LL++ A+ +TPLH AA K + EI LLQ+ ADP+ +++ G TPL L +EG +++ LL + ++ + G T +HL + +++ +A+ L GA++N++ G PLH A +G +M L++ A+V + YTPLH+AAQ+ LL +GA + GQ+PL +
Sbjct: 40 AARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQEN----------------------HEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDS----KGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLL--SRGAII--------DSRTKDLLTPLHCAARSGHDQVVDL--LVVQGAPISAKTKNGLAPLHMAAQGDHVDAART----LLYHRAPVDDVTV---DYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFT---PLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQ-QGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD-KTLLTK------KGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL--------------------IENGSDVGAKANNGL--TAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 4 Score: 148.288 bits (373), Expect = 2.100e-35 Identity = 134/460 (29.13%), Postives = 203/460 (44.13%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELREL--LYFE---------HKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYIL 1561
L ACK N ++VV+ LL + + G TPLH A+ G +V +L+ +GAN ++ G PLH A DVV+VLI G+ +A+ TPLH A+ G D+ I+LLQA A+ N D + +A A+ G EE + IL N + + TPLHLA+ Y +V+LLLE G V + K + PLH A Y + +VA LLE GA A YTPLH AA K + E+ S LL + ADP + +PL L+A + +E+ L E + G + C ++ V ++L G +S + G T +H + N+++FL+ GA + + +PLH A + + ++ L+ N + + TG+ L +A L S+ L
Sbjct: 396 LHIACKKNRIKVVELLLKYRAAIEATTESGL--TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIA---------------------AKEGQEE-VAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTN---------------AGYTPLHVACHFGQL-----NMVKFLVENGA--DVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNE--QTATGQTPLSIAQRLGYVSVVETL 807 HSP 5 Score: 135.576 bits (340), Expect = 1.281e-31 Identity = 105/313 (33.55%), Postives = 160/313 (51.12%), Query Frame = 2
Query: 1850 EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEG-DQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNI------NCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
E L A++AGD+E V +L +DI N + +G +S LH A+ VV+ L++ A V A + G LH A G + + +L++ ANVNV + FTPL+ AA + E+ K LL++ A+ +DG TPL + +++G D+ V LL +DS IA + T+ + N T TPLH+A+ Y + + + LLE+GA+VN Q + + PLH A+ +G +MA LLL A ++S K TPLH AA+ Q+ LL+ GA K G +PL + + D
Sbjct: 32 EGSASFLRAARAGDLEKVLELLRAGTDI-----NTSNANGLNS--LHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGD 337 HSP 6 Score: 115.161 bits (287), Expect = 2.540e-25 Identity = 93/297 (31.31%), Postives = 127/297 (42.76%), Query Frame = 2
Query: 188 ASFEENCRTLFDACKNNDLQVVKCLLDIGYDVN--SKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQELLEAARNGLEE----------TIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLL 1027
A+ +N L A K +V LLD D +K F TPLH AS +G E+V L+ RG V I+ + + PLH A + + V +L+ G+ A YTPLH AA K + ++ LLQ KAD N K+ G T L+ K L+ G ++ A NGL + IL ++ TPLH+A + + +V+ L+E GA V K + PLH A GH + LLE GA N TPL A G V L
Sbjct: 519 ATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGF----TPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENG----SDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Fly
Match: Tnks (gene:FBgn0027508 transcript:FBtr0330383) HSP 1 Score: 734.947 bits (1896), Expect = 0.000e+0 Identity = 417/866 (48.15%), Postives = 560/866 (64.67%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTD--IDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQT 2800
R LF+ACK ++ VK L+ VN++D GRKSTPLHFA+G+GR+E+VEFL++ GA++ DEGGL PLHN CSFGHA+VV++L+ AG+ PN D WNYTPLHEAA KGK DVC+ LLQ A+ ++NS+ T +LA D T+ V TGEY+K ELLEAAR+G E+ ++ +LTP NVNCHASDGR+STPLHLAAGYNR IV++LL GA VHAKDKGGLVPLHNACSYGH +V K L++ GA VNA DLW +TPLHEAASK R EVCSLLL+ GADPT LNCH+KS ++ A T ELRE + FE+KG L + CR + ++ K+++ C+ +++NFVHP+TGDT +H + S + KRK L+E L R+G S LN NK +PLHLA + D +++L++ + ++ L+ + G+ LH A +L++Y ++ I + +G A+QL +D + L + + E AA D + + ++ NN N ++ +G + P+ A+ V +V +LE+ +++ + D G PLH A + V ++L+++GA+V+ D PLH A + G K++ICKLLL++ ADP K+N+DG TP DLVKE D DV +LL SALL+AAKKG +A+VQ+LVT +INCRD QGRNSTPLHLA+GYNN E AE+LLE GADVNAQDKGGLIPLHNASSYGH D+AALL+K+ VN+ DKWG+TPLHEAAQK RTQLCSLLLAHGADA +KN EGQ+P++L +A DV+ LL++A++ +
Sbjct: 25 RELFEACKTGEIAKVKKLI-TPQTVNARDTAGRKSTPLHFAAGYGRREVVEFLLNSGASIQACDEGGLHPLHNCCSFGHAEVVRLLLKAGASPNTTDNWNYTPLHEAASKGKVDVCLALLQHGANHTIRNSEQKTPLELA-DEATRPVLTGEYRKDELLEAARSGAEDRLLALLTPLNVNCHASDGRRSTPLHLAAGYNRIGIVEILLANGADVHAKDKGGLVPLHNACSYGHFDVTKLLIQAGANVNANDLWAFTPLHEAASKSRVEVCSLLLSRGADPTLLNCHSKSAIDAAPTRELRERIAFEYKGHCLLDACRKCDVSRAKKLV----------CA------EIVNFVHPYTGDTPLHLAVVSPD--GKRKQLMELLTRKG--SLLNEKNKAFLTPLHLAAELLHYDAMEVLLK-QGAKVNALDSL-GQTPLHRCAR--DEQAVRLLLSYAADTNIVSLEGLTAAQLASDSVLKLLKNPPDSETHLLEAAKAGDLDTV-----RRIVLNNPI-SVNCRD--LDGRHSTPLHF---------------------AAGFNRVPVVQFLLEHGAEVYAA-----DKGGL--VPLHNACSYGHYEVTELLVKHGANVNVSDLWKFTPLHEAAAKG---------------------------------KYDICKLLLKHGADPMKKNRDGATPADLVKESDHDVAELLRGPSALLDAAKKGNLARVQRLVTPESINCRDAQGRNSTPLHLAAGYNNFECAEYLLENGADVNAQDKGGLIPLHNASSYGHLDIAALLIKHKTVVNATDKWGFTPLHEAAQKGRTQLCSLLLAHGADAYMKNQEGQTPIELATADDVKCLLQDAMATS 795 HSP 2 Score: 344.354 bits (882), Expect = 3.353e-97 Identity = 188/324 (58.02%), Postives = 231/324 (71.30%), Query Frame = 2
Query: 3005 VAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDN--VGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
V+ FL S +L L+ELF +EQ+T+DIL EMGH +LK++GV AYG RHKILKGI++ RST+ G NVN+ C TLLVDLLP D ++ +VEE+MQ++IR H+DN GG F RY I++V+++ N+KLW++Y R+QE+ EEN NER+LFHGSPFI AIV RGFDERHAYIGGMFGAGIYFAE+SSKSNQYVYG+GGG GC HKD+SCY+CPRQ+LLCRVALGK+FLQ + +KMAHAPPGHHSVVGRPS GL +AEYV+YRGEQ+YP +LITY I P
Sbjct: 894 VSGFLSSQQLHHLIELFEREQITLDILAEMGHDDLKQVGVSAYGFRHKILKGIAQL-RSTT---------GIGNNVNL----C---------------------------------TLLVDLLPDDKEFVAVEEEMQATIREHRDNGQAGGYFTRYNIIRVQKVQNRKLWERYAHRRQEIAEENFLQSNERMLFHGSPFINAIVQRGFDERHAYIGGMFGAGIYFAEHSSKSNQYVYGIGGGIGCPSHKDKSCYVCPRQLLLCRVALGKSFLQYSAMKMAHAPPGHHSVVGRPSAGGLHFAEYVVYRGEQSYPEYLITYQIVKP 1170 HSP 3 Score: 253.832 bits (647), Expect = 5.489e-68 Identity = 179/491 (36.46%), Postives = 262/491 (53.36%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFA--SGFG-RKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTIL--TPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEE-INIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDL 1639
+FE L DAC+ D+ K L+ VN + TPLH A S G RK+++E L +G+ ++ K++ L PLH A H D ++VL+ G+ NA D TPLH A +A +LL AD N+ + +GLTA LA+D+ KL+ + LLEAA+ G +T+ I+ P +VNC DGR STPLH AAG+NR +VQ LLE GA+V+A DKGGLVPLHNACSYGH EV + L++ GA VN DLW++TPLHEAA+KG+ ++C LLL +GADP N +P +L + ++ ELL L + + G +V+R++ NC + + T +H NN E+LL GA +N +K G PLH A ++D+ LL++++ +N G T LH AA+ + +C +L+ +G++ + +G +L T D+
Sbjct: 326 AFEYKGHCLLDACRKCDVSRAKKLV-CAEIVNFVHPY-TGDTPLHLAVVSPDGKRKQLMELLTRKGSLLNEKNKAFLTPLHLAAELLHYDAMEVLLKQGAKVNALDSLGQTPLHRCARDEQA--VRLLLSYAADTNIVSLEGLTAAQLASDSVLKLLKNPPDSETHLLEAAKAGDLDTVRRIVLNNPISVNCRDLDGRHSTPLHFAAGFNRVPVVQFLLEHGAEVYAADKGGLVPLHNACSYGHYEVTELLVKHGANVNVSDLWKFTPLHEAAAKGKYDICKLLLKHGADPMKKNRDGATPADLVKESDHDVAELL---RGPSALLDAAKKGNLARVQRLV----TPESINCRDAQGRNS-----------TPLHLAAGYNNF-----ECAEYLLENGA--DVNAQDKGGLIPLHNASSYGHLDIAALLIKHKTVVNATDKWGFT---PLHEAAQKGRTQLCSLLLAHGADAYMKNQEGQTPIELATADDV 784
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Fly
Match: Tnks (gene:FBgn0027508 transcript:FBtr0084890) HSP 1 Score: 733.791 bits (1893), Expect = 0.000e+0 Identity = 418/869 (48.10%), Postives = 561/869 (64.56%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFL-----SEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQT 2800
R LF+ACK ++ VK L+ VN++D GRKSTPLHFA+G+GR+E+VEFL++ GA++ DEGGL PLHN CSFGHA+VV++L+ AG+ PN D WNYTPLHEAA KGK DVC+ LLQ A+ ++NS+ T +LA D T+ V TGEY+K ELLEAAR+G E+ ++ +LTP NVNCHASDGR+STPLHLAAGYNR IV++LL GA VHAKDKGGLVPLHNACSYGH +V K L++ GA VNA DLW +TPLHEAASK R EVCSLLL+ GADPT LNCH+KS ++ A T ELRE + FE+KG L + CR + ++ K+++ C+ +++NFVHP+TGDT +H + S + KRK L+E L R+G S LN NK +PLHLA + D +++L++ + ++ L+ + G+ LH A +L++Y ++ I + +G A+QL +D + + L SE L+ A D+ + ++ NN N ++ +G + P+ A+ V +V +LE+ +++ + D G PLH A + V ++L+++GA+V+ D PLH A + G K++ICKLLL++ ADP K+N+DG TP DLVKE D DV +LL SALL+AAKKG +A+VQ+LVT +INCRD QGRNSTPLHLA+GYNN E AE+LLE GADVNAQDKGGLIPLHNASSYGH D+AALL+K+ VN+ DKWG+TPLHEAAQK RTQLCSLLLAHGADA +KN EGQ+P++L +A DV+ LL++A++ +
Sbjct: 25 RELFEACKTGEIAKVKKLI-TPQTVNARDTAGRKSTPLHFAAGYGRREVVEFLLNSGASIQACDEGGLHPLHNCCSFGHAEVVRLLLKAGASPNTTDNWNYTPLHEAASKGKVDVCLALLQHGANHTIRNSEQKTPLELA-DEATRPVLTGEYRKDELLEAARSGAEDRLLALLTPLNVNCHASDGRRSTPLHLAAGYNRIGIVEILLANGADVHAKDKGGLVPLHNACSYGHFDVTKLLIQAGANVNANDLWAFTPLHEAASKSRVEVCSLLLSRGADPTLLNCHSKSAIDAAPTRELRERIAFEYKGHCLLDACRKCDVSRAKKLV----------CA------EIVNFVHPYTGDTPLHLAVVSPD--GKRKQLMELLTRKG--SLLNEKNKAFLTPLHLAAELLHYDAMEVLLK-QGAKVNALDSL-GQTPLHRCAR--DEQAVRLLLSYAADTNIVSLEGLTAAQLASD-SVLKLLKNPPDSETHLLEAAKAGDLDTVR-------RIVLNNPI-SVNCRD--LDGRHSTPLHF---------------------AAGFNRVPVVQFLLEHGAEVYAA-----DKGGL--VPLHNACSYGHYEVTELLVKHGANVNVSDLWKFTPLHEAAAKG---------------------------------KYDICKLLLKHGADPMKKNRDGATPADLVKESDHDVAELLRGPSALLDAAKKGNLARVQRLVTPESINCRDAQGRNSTPLHLAAGYNNFECAEYLLENGADVNAQDKGGLIPLHNASSYGHLDIAALLIKHKTVVNATDKWGFTPLHEAAQKGRTQLCSLLLAHGADAYMKNQEGQTPIELATADDVKCLLQDAMATS 795 HSP 2 Score: 343.969 bits (881), Expect = 1.636e-98 Identity = 192/339 (56.64%), Postives = 236/339 (69.62%), Query Frame = 2
Query: 2960 DANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDN--VGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
DA+T N V+ FL S +L L+ELF +EQ+T+DIL EMGH +LK++GV AYG RHKILKGI++ RST+ G NVN+ C TLLVDLLP D ++ +VEE+MQ++IR H+DN GG F RY I++V+++ N+KLW++Y R+QE+ EEN NER+LFHGSPFI AIV RGFDERHAYIGGMFGAGIYFAE+SSKSNQYVYG+GGG GC HKD+SCY+CPRQ+LLCRVALGK+FLQ + +KMAHAPPGHHSVVGRPS GL +AEYV+YRGEQ+YP +LITY I P
Sbjct: 887 DADTITN--------VSGFLSSQQLHHLIELFEREQITLDILAEMGHDDLKQVGVSAYGFRHKILKGIAQL-RSTT---------GIGNNVNL----C---------------------------------TLLVDLLPDDKEFVAVEEEMQATIREHRDNGQAGGYFTRYNIIRVQKVQNRKLWERYAHRRQEIAEENFLQSNERMLFHGSPFINAIVQRGFDERHAYIGGMFGAGIYFAEHSSKSNQYVYGIGGGIGCPSHKDKSCYVCPRQLLLCRVALGKSFLQYSAMKMAHAPPGHHSVVGRPSAGGLHFAEYVVYRGEQSYPEYLITYQIVKP 1170 HSP 3 Score: 252.677 bits (644), Expect = 3.283e-68 Identity = 179/491 (36.46%), Postives = 262/491 (53.36%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFA--SGFG-RKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTIL--TPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEE-INIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDL 1639
+FE L DAC+ D+ K L+ VN + TPLH A S G RK+++E L +G+ ++ K++ L PLH A H D ++VL+ G+ NA D TPLH A +A +LL AD N+ + +GLTA LA+D+ KL+ + LLEAA+ G +T+ I+ P +VNC DGR STPLH AAG+NR +VQ LLE GA+V+A DKGGLVPLHNACSYGH EV + L++ GA VN DLW++TPLHEAA+KG+ ++C LLL +GADP N +P +L + ++ ELL L + + G +V+R++ NC + + T +H NN E+LL GA +N +K G PLH A ++D+ LL++++ +N G T LH AA+ + +C +L+ +G++ + +G +L T D+
Sbjct: 326 AFEYKGHCLLDACRKCDVSRAKKLV-CAEIVNFVHPY-TGDTPLHLAVVSPDGKRKQLMELLTRKGSLLNEKNKAFLTPLHLAAELLHYDAMEVLLKQGAKVNALDSLGQTPLHRCARDEQA--VRLLLSYAADTNIVSLEGLTAAQLASDSVLKLLKNPPDSETHLLEAAKAGDLDTVRRIVLNNPISVNCRDLDGRHSTPLHFAAGFNRVPVVQFLLEHGAEVYAADKGGLVPLHNACSYGHYEVTELLVKHGANVNVSDLWKFTPLHEAAAKGKYDICKLLLKHGADPMKKNRDGATPADLVKESDHDVAELL---RGPSALLDAAKKGNLARVQRLV----TPESINCRDAQGRNS-----------TPLHLAAGYNNF-----ECAEYLLENGA--DVNAQDKGGLIPLHNASSYGHLDIAALLIKHKTVVNATDKWGFT---PLHEAAQKGRTQLCSLLLAHGADAYMKNQEGQTPIELATADDV 784
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0344222) HSP 1 Score: 236.884 bits (603), Expect = 2.006e-62 Identity = 235/887 (26.49%), Postives = 367/887 (41.38%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFV------LFNYCRLGEYNKVKRMLQMLGNTN-DSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGAT-------------------------------SSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHS-TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLC 2752
A K+ + VV LL G V+S G +T LH AS G++E+V+ L+ A+V+++ + G PL+ A H VV++L+S G++ + +TPL A +G V VLL++ ++ L A +A K++ ++AA T+L + N + TPLH+A+ Y I LL++ GA V+ K + PLH A +G + LLE+G + A TPLH AA G +V +LL GA + + +PL +AA D R LLY H+ V + + R+ ++L + N D+N G T +H N R ++E LLR GA+ +S +V +G +PLHLA D+I++L+RN +++ E T LH+A+ L + I +L+ +G+ T Y A ++ KE D AA + ++ + K FT P+ T +K G +++ +L+ +D+ D G++ TPLH A +N V +LLE GA HA K G PLH A +A L++Y A N FTPLH ++ +G EI LL+++ A + K+G TP+ L + D N+N +AE L + GA+++ K G PLH AS +G A+M LL+ ANV++A GYTPLH+ AQ+ + +LLL H A+A + GQ+PL +
Sbjct: 51 ASKDGHIHVVSELLRRGAIVDSATKKG--NTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVR----LPALHIAA-------------KKDDVKAA---------TLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLY--HRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANA-------------RALNGFTPLHIACKKN-----RLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQT---PLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALH----------IAAKEGQDEVAAVLIENGAALDAATKKGFT--------------------PLHLT---------------------AKYGHIKVAQLLLQKEADV--------DAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQED--------------------------------NVN----------------------VAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIA 773 HSP 2 Score: 181.415 bits (459), Expect = 2.266e-45 Identity = 194/717 (27.06%), Postives = 316/717 (44.07%), Query Frame = 2
Query: 680 LLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-RNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRA---SQLTTDIDLCEFLSEKEYLDPTAACISDSNK--IVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKE-GDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQG------------------RNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
L AAR G E ++ L N++ + S+ LHLA+ +V LL GA V + K G LH A G EV K LLE A VN +TPL+ AA + V LLL+ GA N+S LA D GF ++KV +L DT + + ++ +K+ ++ L + +V +K G +PLH+A N ++ LL+ + ++N ++ LH+AA+ +++ +L+ G N T G + + + + L E+ A IS K + P + +GE + + D+ +Y + A+ G V + +L+ N+D N R +G TPLH A NR+ VV++LL +GA + A + GL PLH A G + L+Q++A+ +V + TPLH AA + +I ++LL+ A R ++ TPL + G+ D+ LLL A ++ A + + T+++I ++ Q + TPLHL + Y ++++A+ LL++ ADV+AQ K G+ PLH A Y + +A LLL+ A+ ++ K G+TPLH AA+KN+ + + LL +GA A ++ G +PL L S
Sbjct: 15 FLRAARAGNLERVLEHLK-NNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGA--------NQS---LATED-----------GFTPLAVAMQQGHDKVVAVL--------------------------LESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNIS---PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLER------GAPISAKTKNGLAPLHMAA-----------------QGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNAD-----ANARALNG--FTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVD-------ATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSS 642 HSP 3 Score: 150.599 bits (379), Expect = 5.180e-36 Identity = 95/266 (35.71%), Postives = 138/266 (51.88%), Query Frame = 2
Query: 1970 RHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
R TPLH AA N+ ++++LL NGA V A+ + PLH A G+ + LL+Q+ A V+ +T LH AA +G+ E+ +L++ A K G TPL L AK G I KV +L+ + D QG+N TPLH+A YNN ++A LLE+GA +A K G PLH A+ D+A LL+Y A N+ K G+TPLH ++Q+ ++ +LL+ H A G +P+ LC+ D
Sbjct: 435 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLT--------------------AKYGHI-KVAQLLLQKEADV-DAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQED 678 HSP 4 Score: 143.665 bits (361), Expect = 6.591e-34 Identity = 199/838 (23.75%), Postives = 327/838 (39.02%), Query Frame = 2
Query: 527 AAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARN------------GLEETIMTILTP-FNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE---RGAM-------------VNA----------PDLWQ---YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDML---------------------------EGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACIS--------DSNKIVPTFKSKLFTN-----NKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRIL----------------------ENNSDIKSVLV-NCRDTDG---RHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNS-----------TPLHLASGYNNLEIAEFLLEQGA---------------------------------DVNAQDKGGLIPLHNASSYGH 2569
AA G + + L+ D+N N++GL A LA+ + V + ++ ++++A G EE + +L +VN + +G TPL++AA N +V+LLL GA + G PL A GH +V LLE RG + V A PD+ +TPLH A+ G + +LL+ GAD + HN SPL +AA + G+ N V +L+ GN G T +H S + + +++ LL RGA ++ K G +PLH+A ++VD ++L+ + +E+ +D L + G LH+A + N + +L+ +G++ + T G T + + F+ C++ D++ VPT + + + N+TD + ++ D + E + AS+ G+V++V +L E ++ +VL+ N D + TPLH A + I V ++LL+ ADV A+ K G+ PLH AC Y + +VA LL++ A+ + TPLH AA K + +I LL+Y A + +K G TPL L +EG ++ +LL+ A + K + + N+N + +N TPLH+AS + + FLL+ GA + NAQ G PLH A G+
Sbjct: 18 AARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNG--FTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAA---------------------KWGKTNMVSLLLEKGGNIEAKTRD----------------GLTPLHCAARSGH-----EQVVDMLLERGA--PISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGL------TPLHVAAFM----------GCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDI---------------IRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGY 778 HSP 5 Score: 142.895 bits (359), Expect = 1.049e-33 Identity = 107/316 (33.86%), Postives = 157/316 (49.68%), Query Frame = 2
Query: 1868 LEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXK--------KGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
L A++AG++E V L+NN DI N + +G ++ LH A+ I VV LL GA V + K G LH A G +V +LL+++NA+VNV FTPL+ AA + + +LLL A+ +DG TPL + + D +L +S + K K L+ + N T TPLH+AS Y N IA L+++GADVN K + PLH A+ +G +M +LLL+ N+ + + G TPLH AA+ Q+ +LL GA K G +PL + + + R LL
Sbjct: 16 LRAARAGNLERVLEHLKNNIDI-----NTSNANGLNA--LHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILL 324 HSP 6 Score: 119.783 bits (299), Expect = 1.192e-26 Identity = 95/319 (29.78%), Postives = 152/319 (47.65%), Query Frame = 2
Query: 1874 ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTP-LHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLD-LVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTP-----------LHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
A++ G L + + + + +VL+ DT G+ P LH AA + + +LL+N + K G PLH A YG+ +A LLIQ A+VN +PLH AA GK + LLL+ + + +DG TPL + G + V D+LL A + A K +A + +++ + P LH+A+ ++ +A+ LL++ AD NA+ G PLH A + LLL++ A++++ + G TPLH AA + LL H A + G++PL L + A+ +R LL
Sbjct: 139 ATEDGFTPLAVAMQQGHDKVVAVLLES-DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 456 HSP 7 Score: 111.694 bits (278), Expect = 3.933e-24 Identity = 84/294 (28.57%), Postives = 134/294 (45.58%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIG--YDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLAT-DNNTKLVFTGEYKKQELLEAARNGLEET----------IMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLL 1027
+A+ ++ L A K +V L++ G D +K F TPLH + +G ++ + L+ + A+V + + G+ PLH AC + + V +L+ G+ P+A +TPLH AA K + D+ LL+ A N ++ G T L++ + + ++ K + A+NGL + IL N + TPLH+A+ + + +V+ LL+ GA V A G PLH GH + LLE A NA + TPLH A G V L
Sbjct: 496 DATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGF----TPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785 HSP 8 Score: 62.7734 bits (151), Expect = 2.640e-9 Identity = 32/84 (38.10%), Postives = 45/84 (53.57%), Query Frame = 2
Query: 1979 TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLL 2230
TPLH A+ F + ++V+ LL+NGA+V A G PLH GH + LL+++ AN N + TPLH A G + L
Sbjct: 702 TPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785 HSP 9 Score: 58.5362 bits (140), Expect = 5.203e-8 Identity = 44/130 (33.85%), Postives = 65/130 (50.00%), Query Frame = 2
Query: 2423 QGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSA----ADVRFLLEEAISQT 2800
QG +T A+ NLE L+ D+N + GL LH AS GH + + LL+ A V+SA K G T LH A+ + ++ LLL H A +++ G +PL + + A VR LL +Q+
Sbjct: 8 QGDGNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQS 137
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303118) HSP 1 Score: 236.498 bits (602), Expect = 2.395e-62 Identity = 235/887 (26.49%), Postives = 367/887 (41.38%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFV------LFNYCRLGEYNKVKRMLQMLGNTN-DSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGAT-------------------------------SSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHS-TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLC 2752
A K+ + VV LL G V+S G +T LH AS G++E+V+ L+ A+V+++ + G PL+ A H VV++L+S G++ + +TPL A +G V VLL++ ++ L A +A K++ ++AA T+L + N + TPLH+A+ Y I LL++ GA V+ K + PLH A +G + LLE+G + A TPLH AA G +V +LL GA + + +PL +AA D R LLY H+ V + + R+ ++L + N D+N G T +H N R ++E LLR GA+ +S +V +G +PLHLA D+I++L+RN +++ E T LH+A+ L + I +L+ +G+ T Y A ++ KE D AA + ++ + K FT P+ T +K G +++ +L+ +D+ D G++ TPLH A +N V +LLE GA HA K G PLH A +A L++Y A N FTPLH ++ +G EI LL+++ A + K+G TP+ L + D N+N +AE L + GA+++ K G PLH AS +G A+M LL+ ANV++A GYTPLH+ AQ+ + +LLL H A+A + GQ+PL +
Sbjct: 51 ASKDGHIHVVSELLRRGAIVDSATKKG--NTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVR----LPALHIAA-------------KKDDVKAA---------TLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLY--HRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANA-------------RALNGFTPLHIACKKN-----RLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQT---PLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALH----------IAAKEGQDEVAAVLIENGAALDAATKKGFT--------------------PLHLT---------------------AKYGHIKVAQLLLQKEADV--------DAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQED--------------------------------NVN----------------------VAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIA 773 HSP 2 Score: 181.03 bits (458), Expect = 2.330e-45 Identity = 194/717 (27.06%), Postives = 316/717 (44.07%), Query Frame = 2
Query: 680 LLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-RNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRA---SQLTTDIDLCEFLSEKEYLDPTAACISDSNK--IVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKE-GDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQG------------------RNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
L AAR G E ++ L N++ + S+ LHLA+ +V LL GA V + K G LH A G EV K LLE A VN +TPL+ AA + V LLL+ GA N+S LA D GF ++KV +L DT + + ++ +K+ ++ L + +V +K G +PLH+A N ++ LL+ + ++N ++ LH+AA+ +++ +L+ G N T G + + + + L E+ A IS K + P + +GE + + D+ +Y + A+ G V + +L+ N+D N R +G TPLH A NR+ VV++LL +GA + A + GL PLH A G + L+Q++A+ +V + TPLH AA + +I ++LL+ A R ++ TPL + G+ D+ LLL A ++ A + + T+++I ++ Q + TPLHL + Y ++++A+ LL++ ADV+AQ K G+ PLH A Y + +A LLL+ A+ ++ K G+TPLH AA+KN+ + + LL +GA A ++ G +PL L S
Sbjct: 15 FLRAARAGNLERVLEHLK-NNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGA--------NQS---LATED-----------GFTPLAVAMQQGHDKVVAVL--------------------------LESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNIS---PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLER------GAPISAKTKNGLAPLHMAA-----------------QGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNAD-----ANARALNG--FTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVD-------ATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSS 642 HSP 3 Score: 150.599 bits (379), Expect = 5.275e-36 Identity = 95/266 (35.71%), Postives = 138/266 (51.88%), Query Frame = 2
Query: 1970 RHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
R TPLH AA N+ ++++LL NGA V A+ + PLH A G+ + LL+Q+ A V+ +T LH AA +G+ E+ +L++ A K G TPL L AK G I KV +L+ + D QG+N TPLH+A YNN ++A LLE+GA +A K G PLH A+ D+A LL+Y A N+ K G+TPLH ++Q+ ++ +LL+ H A G +P+ LC+ D
Sbjct: 435 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLT--------------------AKYGHI-KVAQLLLQKEADV-DAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQED 678 HSP 4 Score: 143.665 bits (361), Expect = 5.921e-34 Identity = 199/838 (23.75%), Postives = 327/838 (39.02%), Query Frame = 2
Query: 527 AAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARN------------GLEETIMTILTP-FNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE---RGAM-------------VNA----------PDLWQ---YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDML---------------------------EGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACIS--------DSNKIVPTFKSKLFTN-----NKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRIL----------------------ENNSDIKSVLV-NCRDTDG---RHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNS-----------TPLHLASGYNNLEIAEFLLEQGA---------------------------------DVNAQDKGGLIPLHNASSYGH 2569
AA G + + L+ D+N N++GL A LA+ + V + ++ ++++A G EE + +L +VN + +G TPL++AA N +V+LLL GA + G PL A GH +V LLE RG + V A PD+ +TPLH A+ G + +LL+ GAD + HN SPL +AA + G+ N V +L+ GN G T +H S + + +++ LL RGA ++ K G +PLH+A ++VD ++L+ + +E+ +D L + G LH+A + N + +L+ +G++ + T G T + + F+ C++ D++ VPT + + + N+TD + ++ D + E + AS+ G+V++V +L E ++ +VL+ N D + TPLH A + I V ++LL+ ADV A+ K G+ PLH AC Y + +VA LL++ A+ + TPLH AA K + +I LL+Y A + +K G TPL L +EG ++ +LL+ A + K + + N+N + +N TPLH+AS + + FLL+ GA + NAQ G PLH A G+
Sbjct: 18 AARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNG--FTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAA---------------------KWGKTNMVSLLLEKGGNIEAKTRD----------------GLTPLHCAARSGH-----EQVVDMLLERGA--PISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGL------TPLHVAAFM----------GCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDI---------------IRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGY 778 HSP 5 Score: 143.28 bits (360), Expect = 9.348e-34 Identity = 107/316 (33.86%), Postives = 157/316 (49.68%), Query Frame = 2
Query: 1868 LEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXK--------KGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
L A++AG++E V L+NN DI N + +G ++ LH A+ I VV LL GA V + K G LH A G +V +LL+++NA+VNV FTPL+ AA + + +LLL A+ +DG TPL + + D +L +S + K K L+ + N T TPLH+AS Y N IA L+++GADVN K + PLH A+ +G +M +LLL+ N+ + + G TPLH AA+ Q+ +LL GA K G +PL + + + R LL
Sbjct: 16 LRAARAGNLERVLEHLKNNIDI-----NTSNANGLNA--LHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILL 324 HSP 6 Score: 120.168 bits (300), Expect = 1.104e-26 Identity = 95/319 (29.78%), Postives = 152/319 (47.65%), Query Frame = 2
Query: 1874 ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTP-LHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLD-LVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTP-----------LHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
A++ G L + + + + +VL+ DT G+ P LH AA + + +LL+N + K G PLH A YG+ +A LLIQ A+VN +PLH AA GK + LLL+ + + +DG TPL + G + V D+LL A + A K +A + +++ + P LH+A+ ++ +A+ LL++ AD NA+ G PLH A + LLL++ A++++ + G TPLH AA + LL H A + G++PL L + A+ +R LL
Sbjct: 139 ATEDGFTPLAVAMQQGHDKVVAVLLES-DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 456 HSP 7 Score: 111.694 bits (278), Expect = 3.903e-24 Identity = 84/294 (28.57%), Postives = 134/294 (45.58%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIG--YDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLAT-DNNTKLVFTGEYKKQELLEAARNGLEET----------IMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLL 1027
+A+ ++ L A K +V L++ G D +K F TPLH + +G ++ + L+ + A+V + + G+ PLH AC + + V +L+ G+ P+A +TPLH AA K + D+ LL+ A N ++ G T L++ + + ++ K + A+NGL + IL N + TPLH+A+ + + +V+ LL+ GA V A G PLH GH + LLE A NA + TPLH A G V L
Sbjct: 496 DATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGF----TPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785 HSP 8 Score: 62.7734 bits (151), Expect = 2.642e-9 Identity = 32/84 (38.10%), Postives = 45/84 (53.57%), Query Frame = 2
Query: 1979 TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLL 2230
TPLH A+ F + ++V+ LL+NGA+V A G PLH GH + LL+++ AN N + TPLH A G + L
Sbjct: 702 TPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785 HSP 9 Score: 58.5362 bits (140), Expect = 5.435e-8 Identity = 44/130 (33.85%), Postives = 65/130 (50.00%), Query Frame = 2
Query: 2423 QGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSA----ADVRFLLEEAISQT 2800
QG +T A+ NLE L+ D+N + GL LH AS GH + + LL+ A V+SA K G T LH A+ + ++ LLL H A +++ G +PL + + A VR LL +Q+
Sbjct: 8 QGDGNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQS 137
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303122) HSP 1 Score: 236.113 bits (601), Expect = 2.951e-62 Identity = 235/887 (26.49%), Postives = 367/887 (41.38%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFV------LFNYCRLGEYNKVKRMLQMLGNTN-DSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGAT-------------------------------SSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHS-TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLC 2752
A K+ + VV LL G V+S G +T LH AS G++E+V+ L+ A+V+++ + G PL+ A H VV++L+S G++ + +TPL A +G V VLL++ ++ L A +A K++ ++AA T+L + N + TPLH+A+ Y I LL++ GA V+ K + PLH A +G + LLE+G + A TPLH AA G +V +LL GA + + +PL +AA D R LLY H+ V + + R+ ++L + N D+N G T +H N R ++E LLR GA+ +S +V +G +PLHLA D+I++L+RN +++ E T LH+A+ L + I +L+ +G+ T Y A ++ KE D AA + ++ + K FT P+ T +K G +++ +L+ +D+ D G++ TPLH A +N V +LLE GA HA K G PLH A +A L++Y A N FTPLH ++ +G EI LL+++ A + K+G TP+ L + D N+N +AE L + GA+++ K G PLH AS +G A+M LL+ ANV++A GYTPLH+ AQ+ + +LLL H A+A + GQ+PL +
Sbjct: 51 ASKDGHIHVVSELLRRGAIVDSATKKG--NTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVR----LPALHIAA-------------KKDDVKAA---------TLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLY--HRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANA-------------RALNGFTPLHIACKKN-----RLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQT---PLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALH----------IAAKEGQDEVAAVLIENGAALDAATKKGFT--------------------PLHLT---------------------AKYGHIKVAQLLLQKEADV--------DAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQED--------------------------------NVN----------------------VAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIA 773 HSP 2 Score: 180.644 bits (457), Expect = 3.326e-45 Identity = 194/717 (27.06%), Postives = 316/717 (44.07%), Query Frame = 2
Query: 680 LLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-RNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRA---SQLTTDIDLCEFLSEKEYLDPTAACISDSNK--IVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKE-GDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQG------------------RNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
L AAR G E ++ L N++ + S+ LHLA+ +V LL GA V + K G LH A G EV K LLE A VN +TPL+ AA + V LLL+ GA N+S LA D GF ++KV +L DT + + ++ +K+ ++ L + +V +K G +PLH+A N ++ LL+ + ++N ++ LH+AA+ +++ +L+ G N T G + + + + L E+ A IS K + P + +GE + + D+ +Y + A+ G V + +L+ N+D N R +G TPLH A NR+ VV++LL +GA + A + GL PLH A G + L+Q++A+ +V + TPLH AA + +I ++LL+ A R ++ TPL + G+ D+ LLL A ++ A + + T+++I ++ Q + TPLHL + Y ++++A+ LL++ ADV+AQ K G+ PLH A Y + +A LLL+ A+ ++ K G+TPLH AA+KN+ + + LL +GA A ++ G +PL L S
Sbjct: 15 FLRAARAGNLERVLEHLK-NNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGA--------NQS---LATED-----------GFTPLAVAMQQGHDKVVAVL--------------------------LESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNIS---PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLER------GAPISAKTKNGLAPLHMAA-----------------QGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNAD-----ANARALNG--FTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVD-------ATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSS 642 HSP 3 Score: 150.599 bits (379), Expect = 5.288e-36 Identity = 95/266 (35.71%), Postives = 138/266 (51.88%), Query Frame = 2
Query: 1970 RHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
R TPLH AA N+ ++++LL NGA V A+ + PLH A G+ + LL+Q+ A V+ +T LH AA +G+ E+ +L++ A K G TPL L AK G I KV +L+ + D QG+N TPLH+A YNN ++A LLE+GA +A K G PLH A+ D+A LL+Y A N+ K G+TPLH ++Q+ ++ +LL+ H A G +P+ LC+ D
Sbjct: 435 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLT--------------------AKYGHI-KVAQLLLQKEADV-DAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQED 678 HSP 4 Score: 143.28 bits (360), Expect = 8.314e-34 Identity = 199/838 (23.75%), Postives = 327/838 (39.02%), Query Frame = 2
Query: 527 AAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARN------------GLEETIMTILTP-FNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE---RGAM-------------VNA----------PDLWQ---YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDML---------------------------EGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACIS--------DSNKIVPTFKSKLFTN-----NKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRIL----------------------ENNSDIKSVLV-NCRDTDG---RHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNS-----------TPLHLASGYNNLEIAEFLLEQGA---------------------------------DVNAQDKGGLIPLHNASSYGH 2569
AA G + + L+ D+N N++GL A LA+ + V + ++ ++++A G EE + +L +VN + +G TPL++AA N +V+LLL GA + G PL A GH +V LLE RG + V A PD+ +TPLH A+ G + +LL+ GAD + HN SPL +AA + G+ N V +L+ GN G T +H S + + +++ LL RGA ++ K G +PLH+A ++VD ++L+ + +E+ +D L + G LH+A + N + +L+ +G++ + T G T + + F+ C++ D++ VPT + + + N+TD + ++ D + E + AS+ G+V++V +L E ++ +VL+ N D + TPLH A + I V ++LL+ ADV A+ K G+ PLH AC Y + +VA LL++ A+ + TPLH AA K + +I LL+Y A + +K G TPL L +EG ++ +LL+ A + K + + N+N + +N TPLH+AS + + FLL+ GA + NAQ G PLH A G+
Sbjct: 18 AARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNG--FTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAA---------------------KWGKTNMVSLLLEKGGNIEAKTRD----------------GLTPLHCAARSGH-----EQVVDMLLERGA--PISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGL------TPLHVAAFM----------GCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDI---------------IRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGY 778 HSP 5 Score: 142.895 bits (359), Expect = 1.054e-33 Identity = 107/316 (33.86%), Postives = 157/316 (49.68%), Query Frame = 2
Query: 1868 LEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXK--------KGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
L A++AG++E V L+NN DI N + +G ++ LH A+ I VV LL GA V + K G LH A G +V +LL+++NA+VNV FTPL+ AA + + +LLL A+ +DG TPL + + D +L +S + K K L+ + N T TPLH+AS Y N IA L+++GADVN K + PLH A+ +G +M +LLL+ N+ + + G TPLH AA+ Q+ +LL GA K G +PL + + + R LL
Sbjct: 16 LRAARAGNLERVLEHLKNNIDI-----NTSNANGLNA--LHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILL 324 HSP 6 Score: 119.783 bits (299), Expect = 1.395e-26 Identity = 95/319 (29.78%), Postives = 152/319 (47.65%), Query Frame = 2
Query: 1874 ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTP-LHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLD-LVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTP-----------LHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
A++ G L + + + + +VL+ DT G+ P LH AA + + +LL+N + K G PLH A YG+ +A LLIQ A+VN +PLH AA GK + LLL+ + + +DG TPL + G + V D+LL A + A K +A + +++ + P LH+A+ ++ +A+ LL++ AD NA+ G PLH A + LLL++ A++++ + G TPLH AA + LL H A + G++PL L + A+ +R LL
Sbjct: 139 ATEDGFTPLAVAMQQGHDKVVAVLLES-DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 456 HSP 7 Score: 111.309 bits (277), Expect = 4.760e-24 Identity = 84/294 (28.57%), Postives = 134/294 (45.58%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIG--YDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLAT-DNNTKLVFTGEYKKQELLEAARNGLEET----------IMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLL 1027
+A+ ++ L A K +V L++ G D +K F TPLH + +G ++ + L+ + A+V + + G+ PLH AC + + V +L+ G+ P+A +TPLH AA K + D+ LL+ A N ++ G T L++ + + ++ K + A+NGL + IL N + TPLH+A+ + + +V+ LL+ GA V A G PLH GH + LLE A NA + TPLH A G V L
Sbjct: 496 DATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGF----TPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785 HSP 8 Score: 63.1586 bits (152), Expect = 2.464e-9 Identity = 32/84 (38.10%), Postives = 45/84 (53.57%), Query Frame = 2
Query: 1979 TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLL 2230
TPLH A+ F + ++V+ LL+NGA+V A G PLH GH + LL+++ AN N + TPLH A G + L
Sbjct: 702 TPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785 HSP 9 Score: 58.5362 bits (140), Expect = 6.201e-8 Identity = 44/130 (33.85%), Postives = 65/130 (50.00%), Query Frame = 2
Query: 2423 QGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSA----ADVRFLLEEAISQT 2800
QG +T A+ NLE L+ D+N + GL LH AS GH + + LL+ A V+SA K G T LH A+ + ++ LLL H A +++ G +PL + + A VR LL +Q+
Sbjct: 8 QGDGNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQS 137
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Zebrafish
Match: tnksa (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase a [Source:ZFIN;Acc:ZDB-GENE-030131-7450]) HSP 1 Score: 1118.99 bits (2893), Expect = 0.000e+0 Identity = 633/1268 (49.92%), Postives = 832/1268 (65.62%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCS---------LEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T EL+E L +E KG L R + KVK+ L + ++INF HP T +T +H + S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L++YG++ I + QG+ A+Q+ + + + L+E +P S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLD+VKEGD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ + V S++ P + ++ + +N G L G + ++ + L++++++FL+S+ L+ L ++F +EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KGI + G++P + H CS G G T+L+DL P D ++QSVEE++QS+IR H+D N GG+F+RY ILK++++ N+KL ++Y R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 109 RELFEACRNGDVSRVKRLVD-SVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELKERLTYEFKGHSLLQAAREADMTKVKKTLAL----------------EIINFKHPQTHETALHCAVVSPH--PKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDILEVLQKHGAKMNAVDTL----GQTALHRAALAGHLQTCRLLLSYGADPAIVSLQGFTAAQMGNEA-VQQILNEN----------------IPVRNSDV-------DYRLLEAAKAGDL-DTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDMVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSLENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPP------EALPSCFKPQATVVSASVISPGSTPSCLSAASSIDNLAGPLTELAVSGASGPADGATGTERKEGEITILDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERL---------LGGQQGANPYLTFH---CS-------GQG----------------------TVLIDLAPDDKEFQSVEEELQSTIREHRDGGNAGGVFSRYNILKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIIKP 1238
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Zebrafish
Match: tnksa (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase a [Source:ZFIN;Acc:ZDB-GENE-030131-7450]) HSP 1 Score: 1118.99 bits (2893), Expect = 0.000e+0 Identity = 633/1268 (49.92%), Postives = 832/1268 (65.62%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCS---------LEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T EL+E L +E KG L R + KVK+ L + ++INF HP T +T +H + S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L++YG++ I + QG+ A+Q+ + + + L+E +P S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLD+VKEGD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ + V S++ P + ++ + +N G L G + ++ + L++++++FL+S+ L+ L ++F +EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KGI + G++P + H CS G G T+L+DL P D ++QSVEE++QS+IR H+D N GG+F+RY ILK++++ N+KL ++Y R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 109 RELFEACRNGDVSRVKRLVD-SVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELKERLTYEFKGHSLLQAAREADMTKVKKTLAL----------------EIINFKHPQTHETALHCAVVSPH--PKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDILEVLQKHGAKMNAVDTL----GQTALHRAALAGHLQTCRLLLSYGADPAIVSLQGFTAAQMGNEA-VQQILNEN----------------IPVRNSDV-------DYRLLEAAKAGDL-DTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDMVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSLENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPP------EALPSCFKPQATVVSASVISPGSTPSCLSAASSIDNLAGPLTELAVSGASGPADGATGTERKEGEITILDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERL---------LGGQQGANPYLTFH---CS-------GQG----------------------TVLIDLAPDDKEFQSVEEELQSTIREHRDGGNAGGVFSRYNILKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIIKP 1238
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Zebrafish
Match: tnksa (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase a [Source:ZFIN;Acc:ZDB-GENE-030131-7450]) HSP 1 Score: 1118.99 bits (2893), Expect = 0.000e+0 Identity = 633/1268 (49.92%), Postives = 832/1268 (65.62%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCS---------LEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T EL+E L +E KG L R + KVK+ L + ++INF HP T +T +H + S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L++YG++ I + QG+ A+Q+ + + + L+E +P S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLD+VKEGD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ + V S++ P + ++ + +N G L G + ++ + L++++++FL+S+ L+ L ++F +EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KGI + G++P + H CS G G T+L+DL P D ++QSVEE++QS+IR H+D N GG+F+RY ILK++++ N+KL ++Y R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 109 RELFEACRNGDVSRVKRLVD-SVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELKERLTYEFKGHSLLQAAREADMTKVKKTLAL----------------EIINFKHPQTHETALHCAVVSPH--PKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDILEVLQKHGAKMNAVDTL----GQTALHRAALAGHLQTCRLLLSYGADPAIVSLQGFTAAQMGNEA-VQQILNEN----------------IPVRNSDV-------DYRLLEAAKAGDL-DTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDMVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSLENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPP------EALPSCFKPQATVVSASVISPGSTPSCLSAASSIDNLAGPLTELAVSGASGPADGATGTERKEGEITILDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERL---------LGGQQGANPYLTFH---CS-------GQG----------------------TVLIDLAPDDKEFQSVEEELQSTIREHRDGGNAGGVFSRYNILKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIIKP 1238
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Zebrafish
Match: tnksa (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase a [Source:ZFIN;Acc:ZDB-GENE-030131-7450]) HSP 1 Score: 1113.21 bits (2878), Expect = 0.000e+0 Identity = 633/1275 (49.65%), Postives = 830/1275 (65.10%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKST-------PLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCS---------LEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKST PLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T EL+E L +E KG L R + KVK+ L + ++INF HP T +T +H + S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L++YG++ I + QG+ A+Q+ + A N+ +P S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLD+VKEGD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ + V S++ P + ++ + +N G L G + ++ + L++++++FL+S+ L+ L ++F +EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KGI + G++P + H CS G G T+L+DL P D ++QSVEE++QS+IR H+D N GG+F+RY ILK++++ N+KL ++Y R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 130 RELFEACRNGDVSRVKRLVD-SVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTSQKMLSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELKERLTYEFKGHSLLQAAREADMTKVKKTLAL----------------EIINFKHPQTHETALHCAVVSPH--PKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDILEVLQKHGAKMNAVDTL----GQTALHRAALAGHLQTCRLLLSYGADPAIVSLQGFTAAQMGNE-----------------AVQQILNENIPVRNSDV-------DYRLLEAAKAGDL-DTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDMVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSLENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPP------EALPSCFKPQATVVSASVISPGSTPSCLSAASSIDNLAGPLTELAVSGASGPADGATGTERKEGEITILDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERL---------LGGQQGANPYLTFH---CS-------GQG----------------------TVLIDLAPDDKEFQSVEEELQSTIREHRDGGNAGGVFSRYNILKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIIKP 1266
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Zebrafish
Match: tnksb (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b [Source:NCBI gene;Acc:567533]) HSP 1 Score: 1098.58 bits (2840), Expect = 0.000e+0 Identity = 623/1271 (49.02%), Postives = 831/1271 (65.38%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXX------------XXXXXXXXXXXXXRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ +G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH KS +++A T EL+E L +E KG L R + KVK+ Q++I+F HP + D+ +H ++S + KRK + E LLR+GA +++ NK +P H+A + D++++L ++ ++N D L G+ ALH AA C +L++YG++ +I + QG+ ASQ+ + + + L+E VP S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+ LD+VK+GD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+K++ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGA+ +KN EGQ+ LDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + ++G S+ R +GDA L++++++FL+S+ L+ L ++F +EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KGI + G++P + H + T+L+DL D ++QSVEE+MQS+IR H+D N GG+F+RY ++K++++ N+KL ++Y R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCY+C RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 126 RELFEACRNGDVSRVKRLVD-SVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCSGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKTVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHGKSAVDVAPTPELKERLTYEFKGHSLLQAAREADMAKVKKT-----------------AQEIISFKHPHSHDSALHCAVASPH--PKRKQVTELLLRKGA--NIHEKNKDFMTPFHVAAERGHNDVLEVLQKHGAKVNAADTL----GQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTASQMGNEA-VQQILNEN----------------VPPRNSDV-------DYRLLEAAKAGDL-DTVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGANPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSAALI--SPASTPSCLSAASSIDNLAGPLCDGASGGAAGPADGASSTDRKEGDA--------VLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERL---------LGGQQGANPYLTFHCTN--------------------------------QGTVLIDLAVDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKP 1253
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Xenopus
Match: tnks2 (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [Source:NCBI gene;Acc:549762]) HSP 1 Score: 1248.8 bits (3230), Expect = 0.000e+0 Identity = 676/1293 (52.28%), Postives = 882/1293 (68.21%), Query Frame = 2
Query: 146 CDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLL---ERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXX---------XXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNN----CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
C + ++ S A+ E R LF+AC+N D++ V+ L++ +VNS+D GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD ++N+D TA DL +D + K V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL+ GA VHAKDKG LVPLHNACSYGH EV + LL RGA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E+L +E KG L R + +VK+ L + +++NF HP T DT +H SS KRK + E L R+GA S K +PLH+A + D++++L+++ E ++ L+ + G+ +LH AA C +L+N G + +I + QG+ A Q+ + + + L E +P F+N D+ Q+LEA+KAGDVE V ++ + S VNCRD +GR STPLHFAAG+NR++VV+ LL++GADVHAKDKGGLVPLHNACSYGH +V ELL+++ A +NV DLWKFTPLHEAAAKGK+EICKLLLQ+ AD K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +++V+KL T N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL +A DVR LL A+ A + S+ A+ V SS+ TL ++ + ++ + + + + G S + +E+S+ +FL+++ L+ L+++F +EQ+T+D+L+EMGH E KE+G+ AYGHRHKI+KG+ + G +P + +++ + T+L+DL P D ++ SVEE+MQS++R H+D + GG+F +Y ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 6 CAGASGSAASHGDMAAAGEPARELFEACRNGDVERVRKLVNAD-NVNSRDTAGRKSTPLHFAAGFGRKDVVEHLLQSGANVHARDDGGLIPLHNACSFGHAEVVSLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPTIRNTDSRTALDL-SDPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLLCMHMRGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTMLNCHNKSAIDLAPTPQLKEMLSYEFKGHSLLQAAREADLTRVKKHLSL----------------EIVNFKHPQTHDTALHCAASSP--YPKRKQVCELLFRKGANVSEKT--KDFLTPLHVASDKAHNDIVEVLIKH-EAKVNALDNL-GQTSLHRAAHCGHLQTCRLLLNSGCDPSIVSLQGFTALQMGNE-SVQQILQEG----------------LP------FSNTDADR-----------------------------------QLLEAAKAGDVETVKKLCTSQS------VNCRDIEGRQSTPLHFAAGYNRVAVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLVKHGAVINVADLWKFTPLHEAAAKGKYEICKLLLQHGADSTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCVSRVKKLCTPENVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQNGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQAPLDLVTADDVRALLTAAMPPAALSTFYKPQLINVSQPSATAVATLSSVPSSSPTL-----SAASSLDNLASGFSETTSVSSGGAEGASGLDKKEEVPGVELSINQFLQNLGLEHLIDIFEREQITLDVLIEMGHKEFKEIGINAYGHRHKIIKGVERL---------ISGQHGLNPYLTLNTANSG--------------------------------TILIDLAPDDKEFLSVEEEMQSTVREHRDGGHAGGVFTKYNILKIQKVCNKKLWERYTHRRKEVTEENHNHSNERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRP 1164
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Xenopus
Match: tnks2 (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [Source:NCBI gene;Acc:549762]) HSP 1 Score: 1247.26 bits (3226), Expect = 0.000e+0 Identity = 676/1286 (52.57%), Postives = 877/1286 (68.20%), Query Frame = 2
Query: 146 CDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLL---ERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXX------XXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
C + ++ S A+ E R LF+AC+N D++ V+ L++ +VNS+D GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD ++N+D TA DL +D + K V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL+ GA VHAKDKG LVPLHNACSYGH EV + LL RGA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E+L +E KG L R + +VK+ L + +++NF HP T DT +H SS KRK + E L R+GA S K +PLH+A + D++++L+++ E ++ L+ + G+ +LH AA C +L+N G + +I + QG+ A Q+ + + + L E +P F+N D+ Q+LEA+KAGDVE V ++ + S VNCRD +GR STPLHFAAG+NR++VV+ LL++GADVHAKDKGGLVPLHNACSYGH +V ELL+++ A +NV DLWKFTPLHEAAAKGK+EICKLLLQ+ AD K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +++V+KL T N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL +A DVR LL A+ A + I++S + + S + PT S + S S A+ + +E+S+ +FL+++ L+ L+++F +EQ+T+D+L+EMGH E KE+G+ AYGHRHKI+KG+ + G +P + +++ + T+L+DL P D ++ SVEE+MQS++R H+D + GG+F +Y ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 74 CAGASGSAASHGDMAAAGEPARELFEACRNGDVERVRKLVNAD-NVNSRDTAGRKSTPLHFAAGFGRKDVVEHLLQSGANVHARDDGGLIPLHNACSFGHAEVVSLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPTIRNTDSRTALDL-SDPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLLCMHMRGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTMLNCHNKSAIDLAPTPQLKEMLSYEFKGHSLLQAAREADLTRVKKHLSL----------------EIVNFKHPQTHDTALHCAASSP--YPKRKQVCELLFRKGANVSEKT--KDFLTPLHVASDKAHNDIVEVLIKH-EAKVNALDNL-GQTSLHRAAHCGHLQTCRLLLNSGCDPSIVSLQGFTALQMGNE-SVQQILQEG----------------LP------FSNTDADR-----------------------------------QLLEAAKAGDVETVKKLCTSQS------VNCRDIEGRQSTPLHFAAGYNRVAVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLVKHGAVINVADLWKFTPLHEAAAKGKYEICKLLLQHGADSTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCVSRVKKLCTPENVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQNGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQAPLDLVTADDVRALLTAAMPPAALSTFYKPQLINVSQPSATAVATLSSVPSSSPTLSAASSLDNLASGFSETTSVSSGGAEGASGLDKKEEGVELSINQFLQNLGLEHLIDIFEREQITLDVLIEMGHKEFKEIGINAYGHRHKIIKGVERL---------ISGQHGLNPYLTLNTANSG--------------------------------TILIDLAPDDKEFLSVEEEMQSTVREHRDGGHAGGVFTKYNILKIQKVCNKKLWERYTHRRKEVTEENHNHSNERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRP 1230
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Xenopus
Match: tnks2 (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [Source:NCBI gene;Acc:549762]) HSP 1 Score: 1246.49 bits (3224), Expect = 0.000e+0 Identity = 676/1293 (52.28%), Postives = 882/1293 (68.21%), Query Frame = 2
Query: 146 CDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLL---ERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXX---------XXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNN----CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
C + ++ S A+ E R LF+AC+N D++ V+ L++ +VNS+D GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD ++N+D TA DL +D + K V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL+ GA VHAKDKG LVPLHNACSYGH EV + LL RGA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E+L +E KG L R + +VK+ L + +++NF HP T DT +H SS KRK + E L R+GA S K +PLH+A + D++++L+++ E ++ L+ + G+ +LH AA C +L+N G + +I + QG+ A Q+ + + + L E +P F+N D+ Q+LEA+KAGDVE V ++ + S VNCRD +GR STPLHFAAG+NR++VV+ LL++GADVHAKDKGGLVPLHNACSYGH +V ELL+++ A +NV DLWKFTPLHEAAAKGK+EICKLLLQ+ AD K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +++V+KL T N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL +A DVR LL A+ A + S+ A+ V SS+ TL ++ + ++ + + + + G S + +E+S+ +FL+++ L+ L+++F +EQ+T+D+L+EMGH E KE+G+ AYGHRHKI+KG+ + G +P + +++ + T+L+DL P D ++ SVEE+MQS++R H+D + GG+F +Y ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 55 CAGASGSAASHGDMAAAGEPARELFEACRNGDVERVRKLVNAD-NVNSRDTAGRKSTPLHFAAGFGRKDVVEHLLQSGANVHARDDGGLIPLHNACSFGHAEVVSLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPTIRNTDSRTALDL-SDPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLLCMHMRGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTMLNCHNKSAIDLAPTPQLKEMLSYEFKGHSLLQAAREADLTRVKKHLSL----------------EIVNFKHPQTHDTALHCAASSP--YPKRKQVCELLFRKGANVSEKT--KDFLTPLHVASDKAHNDIVEVLIKH-EAKVNALDNL-GQTSLHRAAHCGHLQTCRLLLNSGCDPSIVSLQGFTALQMGNE-SVQQILQEG----------------LP------FSNTDADR-----------------------------------QLLEAAKAGDVETVKKLCTSQS------VNCRDIEGRQSTPLHFAAGYNRVAVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLVKHGAVINVADLWKFTPLHEAAAKGKYEICKLLLQHGADSTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCVSRVKKLCTPENVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQNGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQAPLDLVTADDVRALLTAAMPPAALSTFYKPQLINVSQPSATAVATLSSVPSSSPTL-----SAASSLDNLASGFSETTSVSSGGAEGASGLDKKEEVPGVELSINQFLQNLGLEHLIDIFEREQITLDVLIEMGHKEFKEIGINAYGHRHKIIKGVERL---------ISGQHGLNPYLTLNTANSG--------------------------------TILIDLAPDDKEFLSVEEEMQSTVREHRDGGHAGGVFTKYNILKIQKVCNKKLWERYTHRRKEVTEENHNHSNERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRP 1213
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Xenopus
Match: tnks2 (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [Source:NCBI gene;Acc:549762]) HSP 1 Score: 1241.87 bits (3212), Expect = 0.000e+0 Identity = 672/1275 (52.71%), Postives = 873/1275 (68.47%), Query Frame = 2
Query: 188 ASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLL---ERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXX---------XXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
A+ E R LF+AC+N D++ V+ L++ +VNS+D GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD ++N+D TA DL +D + K V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL+ GA VHAKDKG LVPLHNACSYGH EV + LL RGA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E+L +E KG L R + +VK+ L + +++NF HP T DT +H SS KRK + E L R+GA S K +PLH+A + D++++L+++ E ++ L+ + G+ +LH AA C +L+N G + +I + QG+ A Q+ + + + L E +P F+N D+ Q+LEA+KAGDVE V ++ + S VNCRD +GR STPLHFAAG+NR++VV+ LL++GADVHAKDKGGLVPLHNACSYGH +V ELL+++ A +NV DLWKFTPLHEAAAKGK+EICKLLLQ+ AD K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +++V+KL T N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL +A DVR LL A+ A + S+ A+ V SS+ TL ++ + ++ + + + + G S + + +FL+++ L+ L+++F +EQ+T+D+L+EMGH E KE+G+ AYGHRHKI+KG+ + G +P + +++ + T+L+DL P D ++ SVEE+MQS++R H+D + GG+F +Y ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 33 AAAGEPARELFEACRNGDVERVRKLVNAD-NVNSRDTAGRKSTPLHFAAGFGRKDVVEHLLQSGANVHARDDGGLIPLHNACSFGHAEVVSLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPTIRNTDSRTALDL-SDPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLLCMHMRGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTMLNCHNKSAIDLAPTPQLKEMLSYEFKGHSLLQAAREADLTRVKKHLSL----------------EIVNFKHPQTHDTALHCAASSP--YPKRKQVCELLFRKGANVSEKT--KDFLTPLHVASDKAHNDIVEVLIKH-EAKVNALDNL-GQTSLHRAAHCGHLQTCRLLLNSGCDPSIVSLQGFTALQMGNE-SVQQILQEG----------------LP------FSNTDADR-----------------------------------QLLEAAKAGDVETVKKLCTSQS------VNCRDIEGRQSTPLHFAAGYNRVAVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLVKHGAVINVADLWKFTPLHEAAAKGKYEICKLLLQHGADSTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCVSRVKKLCTPENVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQNGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQAPLDLVTADDVRALLTAAMPPAALSTFYKPQLINVSQPSATAVATLSSVPSSSPTL-----SAASSLDNLASGFSETTSVSSGGAEGASGLDKKEEGKIINQFLQNLGLEHLIDIFEREQITLDVLIEMGHKEFKEIGINAYGHRHKIIKGVERL---------ISGQHGLNPYLTLNTANSG--------------------------------TILIDLAPDDKEFLSVEEEMQSTVREHRDGGHAGGVFTKYNILKIQKVCNKKLWERYTHRRKEVTEENHNHSNERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRP 1173
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Xenopus
Match: tnks2 (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [Source:NCBI gene;Acc:549762]) HSP 1 Score: 1238.79 bits (3204), Expect = 0.000e+0 Identity = 676/1318 (51.29%), Postives = 883/1318 (67.00%), Query Frame = 2
Query: 146 CDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYN----------------------------ADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXX---------XXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNN----CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
C + ++ S A+ E R LF+AC+N D++ V+ L++ +VNS+D GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD ++N+D TA DL +D + K V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL+ GA VHAKDKG LVPLHNACSYGH EV + L++RGA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E+L +E KG L R + +VK+ L + +++NF HP T DT +H SS KRK + E L R+GA S K +PLH+A + D++++L+++ E ++ L+ + G+ +LH AA C +L+N G + +I + QG+ A Q+ + + + L E +P F+N D+ Q+LEA+KAGDVE V ++ + S VNCRD +GR STPLHFAAG+NR++VV+ LL++GADVHAKDKGGLVPLHNACSYGH +V ELL+++ A +NV DLWKFTPLHEAAAKGK+EICKLLLQ N AD K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +++V+KL T N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL +A DVR LL A+ A + S+ A+ V SS+ TL ++ + ++ + + + + G S + +E+S+ +FL+++ L+ L+++F +EQ+T+D+L+EMGH E KE+G+ AYGHRHKI+KG+ + G +P + +++ + T+L+DL P D ++ SVEE+MQS++R H+D + GG+F +Y ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 6 CAGASGSAASHGDMAAAGEPARELFEACRNGDVERVRKLVNAD-NVNSRDTAGRKSTPLHFAAGFGRKDVVEHLLQSGANVHARDDGGLIPLHNACSFGHAEVVSLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPTIRNTDSRTALDL-SDPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKRGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTMLNCHNKSAIDLAPTPQLKEMLSYEFKGHSLLQAAREADLTRVKKHLSL----------------EIVNFKHPQTHDTALHCAASS--PYPKRKQVCELLFRKGANVSEKT--KDFLTPLHVASDKAHNDIVEVLIKH-EAKVNALDNL-GQTSLHRAAHCGHLQTCRLLLNSGCDPSIVSLQGFTALQMGNE-SVQQILQEG----------------LP------FSNTDADR-----------------------------------QLLEAAKAGDVETVKKLCTSQS------VNCRDIEGRQSTPLHFAAGYNRVAVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLVKHGAVINVADLWKFTPLHEAAAKGKYEICKLLLQVNERKGKGDNDDGERGSKSRGGWRGGSEHGADSTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCVSRVKKLCTPENVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQNGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQAPLDLVTADDVRALLTAAMPPAALSTFYKPQLINVSQPSATAVATLSSVPSSSPTL-----SAASSLDNLASGFSETTSVSSGGAEGASGLDKKEEVPGVELSINQFLQNLGLEHLIDIFEREQITLDVLIEMGHKEFKEIGINAYGHRHKIIKGVERL---------ISGQHGLNPYLTLNTANSG--------------------------------TILIDLAPDDKEFLSVEEEMQSTVREHRDGGHAGGVFTKYNILKIQKVCNKKLWERYTHRRKEVTEENHNHSNERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRP 1189
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Mouse
Match: Tnks (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase [Source:MGI Symbol;Acc:MGI:1341087]) HSP 1 Score: 1118.22 bits (2891), Expect = 0.000e+0 Identity = 635/1274 (49.84%), Postives = 830/1274 (65.15%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNN---------CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQ 3994
R L +AC+N D+ VK L+D +VN+KD GRKS+PLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T ELRE L +E KG L R + KVK+ L + ++INF P + +T +H ++S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++ L+ + G+ ALH AA C +L++YGS+ +I + QG+ A+Q+ + + + LSE T SD + +L +K + V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLDLVKEGD D+ DLL D+ALL+AAKKG +A+VQKL T NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ A A V S++ P + ++ + +N G L G +N + L++++++FL+S+ L+ L ++F EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KG+ + G++P + H + T+L+DL P D +YQSVEE+MQS+IR H+D N GGIFNRY +++++++ N+KL +++ R++E+ EEN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P PSQ
Sbjct: 177 RELLEACRNGDVSRVKRLVDAA-NVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLAL----------------EIINFKQPQSHETALHCAVAS--LHPKRKQVAELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDVMEVLHKH-GAKMNALDSL-GQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEA-VQQILSES-----TPMRTSDVD-------YRLLEASKAGDLET------------VKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQAT------VVSASLISPASTPSCLSAASSIDNLTGPLTDLAVGGASNAGDGAAGAERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGTNPYLTFHC--------------------------------VNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEA---PSQ 1311 HSP 2 Score: 255.373 bits (651), Expect = 1.574e-68 Identity = 180/518 (34.75%), Postives = 264/518 (50.97%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + DL VK L + +N K + T LH A RK++ E L+ +GANV+ K++ + PLH A H DV++VL G+ NA D T LH AA+ G C +LL +D ++ + G TA + + +++ + LLEA++ G ET+ + +P NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +V Q L + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L T D+ L + + C +V
Sbjct: 478 TYEFKGHSLLQAAREADLAKVKKTLALEI-INFKQPQSHE-TALHCAVASLHPKRKQVAELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDSLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPMRTSDVDYRLLEASKAGDLETVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCTPENINCRDTQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVV 965
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Mouse
Match: Tnks2 (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [Source:MGI Symbol;Acc:MGI:1921743]) HSP 1 Score: 912.138 bits (2356), Expect = 0.000e+0 Identity = 487/865 (56.30%), Postives = 614/865 (70.98%), Query Frame = 2
Query: 197 EENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAI 2791
E + R LF+AC+N D++ VK L+ VNS+D GRKSTPLHFA+GFGRK++VE+L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ A+ ++N+DG TA DLA D + K V TG+YKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL GA VHAKDKG LVPLHNACSYGH EV + L++ GA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E L +E KG L R + ++K+ L + +++NF HP T +T +H +S KRK + E LLR+GA + N K+ +PLH+A + D+++++V++ E ++ L+ + G+ +LH AA C +L++YG + I + QG+ A Q+ + ++ + L E L + A D+Q+ E A+KAGDVE V ++ S VNCRD +GR STPLHFAAG+NR+SVV+ LL++GADVHAKDKGGLVPLHNACSYGH +VAELL+++ A VNV DLWKFTPLHEAAAKGK+EICKLLLQ+ ADP K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +A+V+KL + N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL SA DV LL A+
Sbjct: 22 EPSARELFEACRNGDVERVKRLV-TPEKVNSRDTAGRKSTPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLQHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLA-DPSAKAVLTGDYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLHHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRIEVCSLLLSYGADPTLLNCHNKSAIDLAPTAQLKERLSYEFKGHSLLQAAREADVTRIKKHLSL----------------EMVNFKHPQTHETALHCAAASP--YPKRKQICELLLRKGANT--NEKTKEFLTPLHVASENAHNDVVEVVVKH-EAKVNALDSL-GQTSLHRAAHCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNE-NVQQLLQEGASLGHSEA--------------------------------------------------DRQLLE-------AAKAGDVETVKKLCTVQS------VNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLTAAM 798 HSP 2 Score: 367.081 bits (941), Expect = 1.360e-106 Identity = 180/342 (52.63%), Postives = 244/342 (71.35%), Query Frame = 2
Query: 2951 TDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
+G + ++ S+ +F+R++ L+ L+++F +EQ+T+D+L+EMGH ELKE+G+ AYGHRHK++KG+ + G +P + ++++ G G T+L+DL P D ++QSVEE+MQS++R H+D + GG+FNRY ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 860 AEGATGLQRKEDSGIDFSITQFIRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERL---------ISGQQGLNPYLTLNNS--------GSG------------------------TILIDLSPDDKEFQSVEEEMQSTVREHRDGGHAGGVFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPIHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIVRP 1160 HSP 3 Score: 312.383 bits (799), Expect = 9.912e-88 Identity = 160/309 (51.78%), Postives = 210/309 (67.96%), Query Frame = 2
Query: 1844 VEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVR 2770
VE ++ EA + GDVE V R++ VN RDT GR STPLHFAAGF R VV+ LL+NGA+V A+D GGL+PLHNACS+GHA+V LL+Q+ A+ N D W +TPLHEAA KGK ++C +LLQ+ A+P RN DG T LDL + V LLE+A+ G K+ L+T +N+NC + GR STPLHLA+GYN ++I + LL GADV+A+DKG L+PLHNA SYGH ++ LL+K+ A VN+ D W +TPLHEAA KNR ++CSLLL++GAD L N +S +DL A ++
Sbjct: 21 VEPSARELFEACRNGDVERVKRLVTPEK------VNSRDTAGRKSTPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLQHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLADPSAKAVLTGDYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLHHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRIEVCSLLLSYGADPTLLNCHNKSAIDLAPTAQLK 323 HSP 4 Score: 260.381 bits (664), Expect = 1.464e-70 Identity = 180/499 (36.07%), Postives = 267/499 (53.51%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTG-----EYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLS 1654
S+E +L A + D+ +K L + VN K + T LH A+ RK++ E L+ +GAN + K + L PLH A H DVV+V++ + NA D T LH AA G C +LL D N+ + G TA + +N +L+ G ++LLEAA+ G ET+ + T +VNC +GR+STPLH AAGYNR +V+ LL+ GA VHAKDKGGLVPLHNACSYGH EVA+ L++ GA+VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +VK+ L + ++ NC + + T +H NN+ + E+LL+ GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L + D+ L+
Sbjct: 327 SYEFKGHSLLQAAREADVTRIKKHLSLEM-VNFK-HPQTHETALHCAAASPYPKRKQICELLLRKGANTNEKTKEFLTPLHVASENAHNDVVEVVVKHEAKVNALDSLGQTSLHRAAHCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGASLGHSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGD---AALLDAAKKGCLARVKK----LSSPDNVNCRDTQGRHS-----------TPLHLAAGYNNL-----EVAEYLLQHGA--DVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLT 795 HSP 5 Score: 229.18 bits (583), Expect = 1.478e-60 Identity = 176/561 (31.37%), Postives = 273/561 (48.66%), Query Frame = 2
Query: 1142 LFNYCRLGEYNKVKRMLQMLG-NTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-----NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGD-QDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGY---NNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAIS 2794
LF CR G+ +VKR++ N+ D+ K ++F F RK+++E+LL+ GA ++ + G PLH A + +++ LL++ N N + LH AA +C +L+ +G+ TI G T +DL + P+ K+ L + K D ++LE++++G+ E + +L + VNC +DGR STPLH AAG+NR+ +V++LL +GADVHAKDKG LVPLHNACSYGH +V ELL+++ A VN DLW+FTPLHEAA+K + E+C LLL Y ADP N + +DL ++ +LL+AA++ + +++K ++ +N + Q + T LH A+ +I E LL +GA+ N + K L PLH AS H D+ +++K+ A VN+ D G T LH AA Q C LLL++G D + + +G + L + + +V+ LL+E S
Sbjct: 28 LFEACRNGDVERVKRLVTPEKVNSRDTAGR----KSTPLHFAAGFG-----------------RKDVVEYLLQNGA--NVQARDDGGLIPLHNACSFGHAEVVNLLLQHGADPNARDNWNY-------TPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDG------RTALDLAD----------------------PSAKAVLTGDYKKD------------------------------------ELLESARSGNEEKMMALL------TPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLHHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRIEVCSLLLSYGADPTLLNCHNKSAIDLAPTAQLKERLSYEFKGHSLLQAAREADVTRIKKHLSLEMVNFKHPQ-THETALHCAAASPYPKRKQICELLLRKGANTNEKTKEFLTPLHVASENAHNDVVEVVVKHEAKVNALDSLGQTSLHRAAHCGHLQTCRLLLSYGCDPNIISLQGFTALQMGN-ENVQQLLQEGAS 486
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Mouse
Match: Tnks2 (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [Source:MGI Symbol;Acc:MGI:1921743]) HSP 1 Score: 264.233 bits (674), Expect = 1.081e-78 Identity = 133/176 (75.57%), Postives = 159/176 (90.34%), Query Frame = 2
Query: 2063 DKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALL 2590
DKGGLVPLHNACSYGH +VAELL+++ A VNV DLWKFTPLHEAAAKGK+EICKLLLQ+ ADP K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +A+V+KL + N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYG D++ALL
Sbjct: 1 DKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGADDVSALL 176 HSP 2 Score: 177.563 bits (449), Expect = 1.189e-48 Identity = 89/179 (49.72%), Postives = 120/179 (67.04%), Query Frame = 2
Query: 398 DEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKL--VFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSL 928
D+GGL+PLHNACS+GH +V ++L+ G+ N D+W +TPLHEAA KGK ++C +LLQ AD KN DG T DL D +T + + G+ LL+AA+ G + + +P NVNC + GR STPLHLAAGYN + + LL+ GA V+A+DKGGL+PLHNA SYG +V+ L
Sbjct: 1 DKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGD---AALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGADDVSALL 176 HSP 3 Score: 121.324 bits (303), Expect = 2.639e-29 Identity = 78/202 (38.61%), Postives = 112/202 (55.45%), Query Frame = 2
Query: 860 DKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV 1459
DKGGLVPLHNACSYGH EVA+ L++ GA+VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +VK+ L + ++ NC + + T +H NN+ + E+LL+ GA +N +K G PLH A D+ LL
Sbjct: 1 DKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGD---AALLDAAKKGCLARVKK----LSSPDNVNCRDTQGRHS-----------TPLHLAAGYNNL-----EVAEYLLQHGA--DVNAQDKGGLIPLHNAASYGADDVSALLT 177 HSP 4 Score: 103.219 bits (256), Expect = 3.782e-23 Identity = 48/82 (58.54%), Postives = 59/82 (71.95%), Query Frame = 2
Query: 2522 DKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADV 2767
DKGGL+PLHNA SYGH ++A LL+K+ A VN AD W +TPLHEAA K + ++C LLL HGAD KN +G +PLDL D
Sbjct: 1 DKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDT 82 HSP 5 Score: 93.5893 bits (231), Expect = 6.455e-20 Identity = 47/89 (52.81%), Postives = 64/89 (71.91%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELL 2131
+L+A+K G + V ++ ++ VNCRDT GRHSTPLH AAG+N + V + LL++GADV+A+DKGGL+PLHNA SYG V+ LL
Sbjct: 94 LLDAAKKGCLARVKKLSSPDN------VNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGADDVSALL 176 HSP 6 Score: 80.8777 bits (198), Expect = 1.242e-15 Identity = 49/110 (44.55%), Postives = 66/110 (60.00%), Query Frame = 2
Query: 212 TLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKG 541
L DA K L VK L +VN +D GR STPLH A+G+ E+ E+L+ GA+V+ +D+GGLIPLHNA S+G AD V L++A P+A Y P + V+G
Sbjct: 93 ALLDAAKKGCLARVKKLSSPD-NVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYG-ADDVSALLTAAMPPSALPTC-YKPQVLSGVRG 199 HSP 7 Score: 80.4925 bits (197), Expect = 1.520e-15 Identity = 30/74 (40.54%), Postives = 54/74 (72.97%), Query Frame = 2
Query: 2951 TDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKF 3172
+G + ++ S+ +F+R++ L+ L+++F +EQ+T+D+L+EMGH ELKE+G+ AYGHRHK++KG+ +
Sbjct: 242 AEGATGLQRKEDSGIDFSITQFIRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERL 315 HSP 8 Score: 52.373 bits (124), Expect = 2.238e-6 Identity = 27/69 (39.13%), Postives = 40/69 (57.97%), Query Frame = 2
Query: 2438 TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPL 2644
PLH A Y + E+AE L++ GA VN D PLH A++ G ++ LLL++ A+ ++ G TPL
Sbjct: 6 VPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPL 74
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Mouse
Match: Tnks (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase [Source:MGI Symbol;Acc:MGI:1341087]) HSP 1 Score: 221.09 bits (562), Expect = 3.652e-66 Identity = 112/149 (75.17%), Postives = 131/149 (87.92%), Query Frame = 2
Query: 3485 RLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQA 3931
++ N+KL +++ R++E+ EEN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQ
Sbjct: 2 KVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQV 150
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025]) HSP 1 Score: 236.113 bits (601), Expect = 6.064e-63 Identity = 244/878 (27.79%), Postives = 370/878 (42.14%), Query Frame = 2
Query: 212 TLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLAT------------DNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKS-----------TPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHS-------TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLC 2752
+ A + +L V L G D+N+ + G + LH A+ G +V+ L+ RG++V + G LH A G A+VVKVL+ G++ NA+ +TPL+ AA + DV LL+ A+ + DG T +A +N+TK G+ + L AAR ++ +L N H +D + TPLH+AA Y + LLL GA V + G+ PLH A G+ + K LL+RG ++A TPLH AA G +V LLL A + SPL +AA + E C VK +LQ +D T +H + + + LL + A + LN G +PLH+A + +++LLV+ + E +G +H+AA + +I +L+ G++ + +G A + E + C+ + +V + + E P ++ KT + + ++ A+ G L E D+ SVL+ + HS TPLH AA + + V K+LL+ A + K GL PLH A Y + KVA LL++ A+ + +TPLH AA K + +I LL Y A+ + K G TPL L +EG D+ LL L NI+ G S LHLA+ + + +A+ L + GAD +A K G PL A YG+ M LLK ANVN+ K GYTPLH+AAQ+ T + ++LL HGA G + L +
Sbjct: 13 SFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNA--LHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTK----GKVRLPALHIAARKDDTKSAALLLQ----NDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVE--------------C-------VKHLLQYKAPVDDVTLDYL----------------TALHVAAHCGHY-----RVTKLLLDKRANPNARALN--GFTPLHIACKKNRIKVMELLVKYGASIQAITE--SGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVR----------CLLRNGALV-------------------DARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLL---EAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLL--------------------LDKGANIHMSTKSGLTS--LHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIA 780 HSP 2 Score: 192.586 bits (488), Expect = 4.344e-49 Identity = 222/830 (26.75%), Postives = 350/830 (42.17%), Query Frame = 2
Query: 350 EMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDL-ATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE--------RGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDL-CSAADVR---FLLEEA 2788
++VE+L G +++ ++ GL LH A GH +V+ L+ GS ++ T LH A++ G+A+V VL++ A++N ++ +G T + A +N+ +V + LLE NG ++ T DG + L G+N+ V +LLE K K L LH A + A LL+ MVN +TPLH AA G V +LLL GA + +PL +A+ + G N VK +L G ID + G T +H S + ++E LL R A L K G SPLH+A +V+ ++ L++ +++ +D L ALH+AA + +L++ +N G+ + + + + + I++S + P + + N + +P V + E + A++AG VE+V +L N + LV+ R + TPLH A+ + +V++LL++ A A G PLH + G VA +L++ A ++ FTPLH AA G ++ KLLLQ A K+G TPL + D KV L+ + T TPLH+A+ N ++IA LL GA+ N K G+ PLH AS GH DM LLL AN++ + K G T LH AAQ+++ + +L HGAD G +PL + C +V+ FLL++
Sbjct: 25 KVVEYL-KGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVV-------KYLLE---NGANQSTAT-----------EDGFTPLAVALQQGHNQA--VAILLENDT----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVAS---------------------KRGNTNMVKLLLDRGGQ---------IDAKT-------RDGLTPLHCAARSGH-----DQVVELLLERKA--PLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYL------TALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESG-LTPIHVAAFMGH-----LNIVLLLLQNGASPDVTNIRGETALH------------MAARAGQVEVVRCLLRNGA-----LVDARARE--EQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQ---------------------KVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQG 730 HSP 3 Score: 156.762 bits (395), Expect = 5.532e-38 Identity = 186/695 (26.76%), Postives = 283/695 (40.72%), Query Frame = 2
Query: 149 DKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-----------------------NEEINIDMLEG-----VTGKNA---LHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTD---IDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELL 2131
D AD + + E L A ++ V LL+ G V+ G TPLH AS G MV+ L+ RG + K GL PLH A GH VV++L+ + A+ +PLH AA + LLQ KA V+ D LTA +A G Y+ +LL R N N A +G TPLH+A NR ++++LL++ GA + A + GL P+H A GH+ + LL+ GA + ++ T LH AA G+ EV LL GA ++PL +A+ RLG+ V+ +LQ + + D+ +N G T +H + V ++ LL GA SL K+G +PLH+A ++D+ +LL++ N+++ + +LE T KN LH+AA+ N I L+NYG+ T QG L + D+ L +K A I S K + + L + DK N + K D + ++ A G+V++V +L+ ++ VN + +G TPLH AA ++ VLL++GA +A G L A G+ V + L
Sbjct: 194 DHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNG--ITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVA-------AHCGHYRVTKLLLDKR-------------ANPNARALNG--FTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIAS---------------------RLGKTEIVQLLLQHMAHP-DAATTN---------------GYTPLHISAREGQV-----DVASVLLEAGAAHSLAT--KKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDK------GANIHMSTK---SGLTSLHLAAQEDKVNV------------ADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGAN-----VNAKTKNGY--TPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792 HSP 4 Score: 53.9138 bits (128), Expect = 1.755e-6 Identity = 38/127 (29.92%), Postives = 69/127 (54.33%), Query Frame = 2
Query: 2432 NSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLLEEAISQT 2800
N++ L A N ++ E+L + G D+N ++ GL LH A+ GH + LL ++V+SA K G T LH A+ + ++ +L+ GA+ ++ G +PL + + + V++LLE +Q+
Sbjct: 11 NASFLRAARAGNLDKVVEYL-KGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQS 136
BLAST of Poly [ADP-ribose] polymerase vs. UniProt/SwissProt
Match: sp|O95271|TNKS1_HUMAN (Poly [ADP-ribose] polymerase tankyrase-1 OS=Homo sapiens OX=9606 GN=TNKS PE=1 SV=2) HSP 1 Score: 1122.07 bits (2901), Expect = 0.000e+0 Identity = 637/1272 (50.08%), Postives = 834/1272 (65.57%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRT---DGDANTSNNNN--CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQ 3994
R L +AC+N D+ VK L+D +VN+KD GRKS+PLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T ELRE L +E KG L R + KVK+ L + ++INF P + +T +H ++S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L++YGS+ +I + QG+ A+Q+ + + + LSE T SD + +L +K + V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLDLVKEGD D+ DLL D+ALL+AAKKG +A+VQKL T NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + DG A T L++++++FL+S+ L+ L ++F EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KG+ + G++P + H + T+L+DL P D +YQSVEE+MQS+IR H+D N GGIFNRY +++++++ N+KL +++ R++E+ EEN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P PSQ
Sbjct: 184 RELLEACRNGDVSRVKRLVDAA-NVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLAL----------------EIINFKQPQSHETALHCAVAS--LHPKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTL----GQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEA-VQQILSES-----TPIRTSDVD-------YRLLEASKAGDLET------------VKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASLI--SPASTPSCLSAASSIDNLTGPLAELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGTNPYLTFHC--------------------------------VNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEA---PSQ 1318 HSP 2 Score: 251.906 bits (642), Expect = 1.337e-66 Identity = 179/518 (34.56%), Postives = 263/518 (50.77%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + DL VK L + +N K + T LH A RK++ E L+ +GANV+ K++ + PLH A H DV++VL G+ NA D T LH AA+ G C +LL +D ++ + G TA + + +++ + LLEA++ G ET+ + + NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +V Q L + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L T D+ L + + C +V
Sbjct: 485 TYEFKGHSLLQAAREADLAKVKKTLALEI-INFKQPQSHE-TALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCTPENINCRDTQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVV 972
BLAST of Poly [ADP-ribose] polymerase vs. UniProt/SwissProt
Match: sp|Q6PFX9|TNKS1_MOUSE (Poly [ADP-ribose] polymerase tankyrase-1 OS=Mus musculus OX=10090 GN=Tnks PE=1 SV=1) HSP 1 Score: 1118.22 bits (2891), Expect = 0.000e+0 Identity = 635/1274 (49.84%), Postives = 830/1274 (65.15%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNN---------CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQ 3994
R L +AC+N D+ VK L+D +VN+KD GRKS+PLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T ELRE L +E KG L R + KVK+ L + ++INF P + +T +H ++S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++ L+ + G+ ALH AA C +L++YGS+ +I + QG+ A+Q+ + + + LSE T SD + +L +K + V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLDLVKEGD D+ DLL D+ALL+AAKKG +A+VQKL T NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ A A V S++ P + ++ + +N G L G +N + L++++++FL+S+ L+ L ++F EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KG+ + G++P + H + T+L+DL P D +YQSVEE+MQS+IR H+D N GGIFNRY +++++++ N+KL +++ R++E+ EEN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P PSQ
Sbjct: 177 RELLEACRNGDVSRVKRLVDAA-NVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLAL----------------EIINFKQPQSHETALHCAVAS--LHPKRKQVAELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDVMEVLHKH-GAKMNALDSL-GQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEA-VQQILSES-----TPMRTSDVD-------YRLLEASKAGDLET------------VKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQAT------VVSASLISPASTPSCLSAASSIDNLTGPLTDLAVGGASNAGDGAAGAERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGTNPYLTFHC--------------------------------VNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEA---PSQ 1311 HSP 2 Score: 255.373 bits (651), Expect = 1.101e-67 Identity = 180/518 (34.75%), Postives = 264/518 (50.97%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + DL VK L + +N K + T LH A RK++ E L+ +GANV+ K++ + PLH A H DV++VL G+ NA D T LH AA+ G C +LL +D ++ + G TA + + +++ + LLEA++ G ET+ + +P NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +V Q L + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L T D+ L + + C +V
Sbjct: 478 TYEFKGHSLLQAAREADLAKVKKTLALEI-INFKQPQSHE-TALHCAVASLHPKRKQVAELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDSLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPMRTSDVDYRLLEASKAGDLETVKQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCTPENINCRDTQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVV 965
BLAST of Poly [ADP-ribose] polymerase vs. UniProt/SwissProt
Match: sp|Q3UES3|TNKS2_MOUSE (Poly [ADP-ribose] polymerase tankyrase-2 OS=Mus musculus OX=10090 GN=Tnks2 PE=2 SV=2) HSP 1 Score: 912.138 bits (2356), Expect = 0.000e+0 Identity = 487/865 (56.30%), Postives = 614/865 (70.98%), Query Frame = 2
Query: 197 EENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAI 2791
E + R LF+AC+N D++ VK L+ VNS+D GRKSTPLHFA+GFGRK++VE+L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ A+ ++N+DG TA DLA D + K V TG+YKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL GA VHAKDKG LVPLHNACSYGH EV + L++ GA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E L +E KG L R + ++K+ L + +++NF HP T +T +H +S KRK + E LLR+GA + N K+ +PLH+A + D+++++V++ E ++ L+ + G+ +LH AA C +L++YG + I + QG+ A Q+ + ++ + L E L + A D+Q+ E A+KAGDVE V ++ S VNCRD +GR STPLHFAAG+NR+SVV+ LL++GADVHAKDKGGLVPLHNACSYGH +VAELL+++ A VNV DLWKFTPLHEAAAKGK+EICKLLLQ+ ADP K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +A+V+KL + N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL SA DV LL A+
Sbjct: 22 EPSARELFEACRNGDVERVKRLV-TPEKVNSRDTAGRKSTPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLQHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLA-DPSAKAVLTGDYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLHHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRIEVCSLLLSYGADPTLLNCHNKSAIDLAPTAQLKERLSYEFKGHSLLQAAREADVTRIKKHLSL----------------EMVNFKHPQTHETALHCAAASP--YPKRKQICELLLRKGANT--NEKTKEFLTPLHVASENAHNDVVEVVVKH-EAKVNALDSL-GQTSLHRAAHCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNE-NVQQLLQEGASLGHSEA--------------------------------------------------DRQLLE-------AAKAGDVETVKKLCTVQS------VNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLTAAM 798 HSP 2 Score: 367.081 bits (941), Expect = 9.514e-106 Identity = 180/342 (52.63%), Postives = 244/342 (71.35%), Query Frame = 2
Query: 2951 TDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
+G + ++ S+ +F+R++ L+ L+++F +EQ+T+D+L+EMGH ELKE+G+ AYGHRHK++KG+ + G +P + ++++ G G T+L+DL P D ++QSVEE+MQS++R H+D + GG+FNRY ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 860 AEGATGLQRKEDSGIDFSITQFIRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERL---------ISGQQGLNPYLTLNNS--------GSG------------------------TILIDLSPDDKEFQSVEEEMQSTVREHRDGGHAGGVFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPIHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIVRP 1160 HSP 3 Score: 312.383 bits (799), Expect = 6.933e-87 Identity = 160/309 (51.78%), Postives = 210/309 (67.96%), Query Frame = 2
Query: 1844 VEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVR 2770
VE ++ EA + GDVE V R++ VN RDT GR STPLHFAAGF R VV+ LL+NGA+V A+D GGL+PLHNACS+GHA+V LL+Q+ A+ N D W +TPLHEAA KGK ++C +LLQ+ A+P RN DG T LDL + V LLE+A+ G K+ L+T +N+NC + GR STPLHLA+GYN ++I + LL GADV+A+DKG L+PLHNA SYGH ++ LL+K+ A VN+ D W +TPLHEAA KNR ++CSLLL++GAD L N +S +DL A ++
Sbjct: 21 VEPSARELFEACRNGDVERVKRLVTPEK------VNSRDTAGRKSTPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLQHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLADPSAKAVLTGDYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLHHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRIEVCSLLLSYGADPTLLNCHNKSAIDLAPTAQLK 323 HSP 4 Score: 260.381 bits (664), Expect = 1.024e-69 Identity = 180/499 (36.07%), Postives = 267/499 (53.51%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTG-----EYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLS 1654
S+E +L A + D+ +K L + VN K + T LH A+ RK++ E L+ +GAN + K + L PLH A H DVV+V++ + NA D T LH AA G C +LL D N+ + G TA + +N +L+ G ++LLEAA+ G ET+ + T +VNC +GR+STPLH AAGYNR +V+ LL+ GA VHAKDKGGLVPLHNACSYGH EVA+ L++ GA+VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +VK+ L + ++ NC + + T +H NN+ + E+LL+ GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L + D+ L+
Sbjct: 327 SYEFKGHSLLQAAREADVTRIKKHLSLEM-VNFK-HPQTHETALHCAAASPYPKRKQICELLLRKGANTNEKTKEFLTPLHVASENAHNDVVEVVVKHEAKVNALDSLGQTSLHRAAHCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGASLGHSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGD---AALLDAAKKGCLARVKK----LSSPDNVNCRDTQGRHS-----------TPLHLAAGYNNL-----EVAEYLLQHGA--DVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLT 795 HSP 5 Score: 229.18 bits (583), Expect = 1.034e-59 Identity = 176/561 (31.37%), Postives = 273/561 (48.66%), Query Frame = 2
Query: 1142 LFNYCRLGEYNKVKRMLQMLG-NTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-----NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGD-QDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGY---NNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAIS 2794
LF CR G+ +VKR++ N+ D+ K ++F F RK+++E+LL+ GA ++ + G PLH A + +++ LL++ N N + LH AA +C +L+ +G+ TI G T +DL + P+ K+ L + K D ++LE++++G+ E + +L + VNC +DGR STPLH AAG+NR+ +V++LL +GADVHAKDKG LVPLHNACSYGH +V ELL+++ A VN DLW+FTPLHEAA+K + E+C LLL Y ADP N + +DL ++ +LL+AA++ + +++K ++ +N + Q + T LH A+ +I E LL +GA+ N + K L PLH AS H D+ +++K+ A VN+ D G T LH AA Q C LLL++G D + + +G + L + + +V+ LL+E S
Sbjct: 28 LFEACRNGDVERVKRLVTPEKVNSRDTAGR----KSTPLHFAAGFG-----------------RKDVVEYLLQNGA--NVQARDDGGLIPLHNACSFGHAEVVNLLLQHGADPNARDNWNY-------TPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDG------RTALDLAD----------------------PSAKAVLTGDYKKD------------------------------------ELLESARSGNEEKMMALL------TPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLHHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRIEVCSLLLSYGADPTLLNCHNKSAIDLAPTAQLKERLSYEFKGHSLLQAAREADVTRIKKHLSLEMVNFKHPQ-THETALHCAAASPYPKRKQICELLLRKGANTNEKTKEFLTPLHVASENAHNDVVEVVVKHEAKVNALDSLGQTSLHRAAHCGHLQTCRLLLSYGCDPNIISLQGFTALQMGN-ENVQQLLQEGAS 486
BLAST of Poly [ADP-ribose] polymerase vs. UniProt/SwissProt
Match: sp|Q9H2K2|TNKS2_HUMAN (Poly [ADP-ribose] polymerase tankyrase-2 OS=Homo sapiens OX=9606 GN=TNKS2 PE=1 SV=1) HSP 1 Score: 756.133 bits (1951), Expect = 0.000e+0 Identity = 431/863 (49.94%), Postives = 568/863 (65.82%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMK---EKTTNDKQVEEYCMQILE-ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAI 2791
LF+AC+N D++ VK L+ VNS+D GRKSTPLHFA+GFGRK++VE+L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ A+ ++N+DG TA DLA D + K V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR +IVQLLL+ GA VHAKDKG LVPLHNACSYGH EV + L++ GA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCHNKS ++LA T +L+E L +E KG L R + ++K+ L + +++NF HP T +T +H +S KRK + E LLR+GA ++N K+ +PLH+A + D+++++V++ E ++ L+ + G+ +LH AA C +L++YG + I + QG+ A Q+ + ++ + L E L N++ D+ E A VE+ K ++ N + +E L A+ V +V +L++ +D V+ +D G PLH A + V ++L+++GA V+ D PLH A + G ++ +L LLQ+ ADP K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +A+V+KL + N+NCRDTQGR+STPLHLA+GYNNLE+AE+LL+ GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+A VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD LKN EGQ+PLDL SA DV LL A+
Sbjct: 28 LFEACRNGDVERVKRLV-TPEKVNSRDTAGRKSTPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLA-DPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKHLSL----------------EMVNFKHPQTHETALHCAAASP--YPKRKQICELLLRKGA--NINEKTKEFLTPLHVASEKAHNDVVEVVVKH-EAKVNALDNL-GQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNE-NVQQLLQEGISLG----------------------NSEADRQ-----LLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKL---------------------------------LLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLTAAM 798 HSP 2 Score: 367.466 bits (942), Expect = 8.219e-106 Identity = 182/342 (53.22%), Postives = 246/342 (71.93%), Query Frame = 2
Query: 2951 TDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
T+G ++ ++ S+ +F+R++ L+ L+++F +EQ+T+D+L+EMGH ELKE+G+ AYGHRHK++KG+ + G +P + ++++ G G T+L+DL P D ++QSVEE+MQS++R H+D + GGIFNRY ILK++++CN+KLW++Y R++E+ EEN N NER+LFHGSPF+ AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 860 TEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERL---------ISGQQGLNPYLTLNTS--------GSG------------------------TILIDLSPDDKEFQSVEEEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRP 1160 HSP 3 Score: 260.766 bits (665), Expect = 8.797e-70 Identity = 179/499 (35.87%), Postives = 268/499 (53.71%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTG-----EYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLS 1654
++E +L A + D+ +K L + VN K + T LH A+ RK++ E L+ +GAN++ K + L PLH A H DVV+V++ + NA D T LH AA G C +LL D N+ + G TA + +N +L+ G ++LLEAA+ G ET+ + T +VNC +GR+STPLH AAGYNR +V+ LL+ GA VHAKDKGGLVPLHNACSYGH EVA+ L++ GA+VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +VK+ L + ++ NC + + T +H NN+ + E+LL+ GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G L + D+ L+
Sbjct: 327 AYEFKGHSLLQAAREADVTRIKKHLSLEM-VNFK-HPQTHETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVVKHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGISLGNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGD---AALLDAAKKGCLARVKK----LSSPDNVNCRDTQGRHS-----------TPLHLAAGYNNL-----EVAEYLLQHGA--DVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLT 795 HSP 4 Score: 232.646 bits (592), Expect = 7.772e-61 Identity = 178/561 (31.73%), Postives = 275/561 (49.02%), Query Frame = 2
Query: 1142 LFNYCRLGEYNKVKRMLQMLG-NTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-----NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGD-QDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGY---NNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAIS 2794
LF CR G+ +VKR++ N+ D+ K ++F F RK+++E+LL+ GA ++ + G PLH A + +++ LL+R N N + LH AA +C +L+ +G+ TI G T +DL + P+ K+ L K D ++LE++++G+ E + +L + VNC +DGR STPLH AAG+NR+ +V++LL++GADVHAKDKG LVPLHNACSYGH +V ELL+++ A VN DLW+FTPLHEAA+K + E+C LLL Y ADP N + +DL ++ +LL+AA++ + +++K ++ +N + Q + T LH A+ +I E LL +GA++N + K L PLH AS H D+ +++K+ A VN+ D G T LH AA Q C LLL++G D + + +G + L + + +V+ LL+E IS
Sbjct: 28 LFEACRNGDVERVKRLVTPEKVNSRDTAGR----KSTPLHFAAGFG-----------------RKDVVEYLLQNGA--NVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNY-------TPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDG------RTALDLAD----------------------PSAKAVLTGEYKKD------------------------------------ELLESARSGNEEKMMALL------TPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKHLSLEMVNFKHPQ-THETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVVKHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGN-ENVQQLLQEGIS 486
BLAST of Poly [ADP-ribose] polymerase vs. UniProt/SwissProt
Match: sp|Q9VBP3|TNKS_DROME (Poly [ADP-ribose] polymerase tankyrase OS=Drosophila melanogaster OX=7227 GN=Tnks PE=1 SV=1) HSP 1 Score: 733.791 bits (1893), Expect = 0.000e+0 Identity = 418/869 (48.10%), Postives = 561/869 (64.56%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFL-----SEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQT 2800
R LF+ACK ++ VK L+ VN++D GRKSTPLHFA+G+GR+E+VEFL++ GA++ DEGGL PLHN CSFGHA+VV++L+ AG+ PN D WNYTPLHEAA KGK DVC+ LLQ A+ ++NS+ T +LA D T+ V TGEY+K ELLEAAR+G E+ ++ +LTP NVNCHASDGR+STPLHLAAGYNR IV++LL GA VHAKDKGGLVPLHNACSYGH +V K L++ GA VNA DLW +TPLHEAASK R EVCSLLL+ GADPT LNCH+KS ++ A T ELRE + FE+KG L + CR + ++ K+++ C+ +++NFVHP+TGDT +H + S + KRK L+E L R+G S LN NK +PLHLA + D +++L++ + ++ L+ + G+ LH A +L++Y ++ I + +G A+QL +D + + L SE L+ A D+ + ++ NN N ++ +G + P+ A+ V +V +LE+ +++ + D G PLH A + V ++L+++GA+V+ D PLH A + G K++ICKLLL++ ADP K+N+DG TP DLVKE D DV +LL SALL+AAKKG +A+VQ+LVT +INCRD QGRNSTPLHLA+GYNN E AE+LLE GADVNAQDKGGLIPLHNASSYGH D+AALL+K+ VN+ DKWG+TPLHEAAQK RTQLCSLLLAHGADA +KN EGQ+P++L +A DV+ LL++A++ +
Sbjct: 25 RELFEACKTGEIAKVKKLI-TPQTVNARDTAGRKSTPLHFAAGYGRREVVEFLLNSGASIQACDEGGLHPLHNCCSFGHAEVVRLLLKAGASPNTTDNWNYTPLHEAASKGKVDVCLALLQHGANHTIRNSEQKTPLELA-DEATRPVLTGEYRKDELLEAARSGAEDRLLALLTPLNVNCHASDGRRSTPLHLAAGYNRIGIVEILLANGADVHAKDKGGLVPLHNACSYGHFDVTKLLIQAGANVNANDLWAFTPLHEAASKSRVEVCSLLLSRGADPTLLNCHSKSAIDAAPTRELRERIAFEYKGHCLLDACRKCDVSRAKKLV----------CA------EIVNFVHPYTGDTPLHLAVVSPD--GKRKQLMELLTRKG--SLLNEKNKAFLTPLHLAAELLHYDAMEVLLK-QGAKVNALDSL-GQTPLHRCAR--DEQAVRLLLSYAADTNIVSLEGLTAAQLASD-SVLKLLKNPPDSETHLLEAAKAGDLDTVR-------RIVLNNPI-SVNCRD--LDGRHSTPLHF---------------------AAGFNRVPVVQFLLEHGAEVYAA-----DKGGL--VPLHNACSYGHYEVTELLVKHGANVNVSDLWKFTPLHEAAAKG---------------------------------KYDICKLLLKHGADPMKKNRDGATPADLVKESDHDVAELLRGPSALLDAAKKGNLARVQRLVTPESINCRDAQGRNSTPLHLAAGYNNFECAEYLLENGADVNAQDKGGLIPLHNASSYGHLDIAALLIKHKTVVNATDKWGFTPLHEAAQKGRTQLCSLLLAHGADAYMKNQEGQTPIELATADDVKCLLQDAMATS 795 HSP 2 Score: 343.969 bits (881), Expect = 1.640e-97 Identity = 192/339 (56.64%), Postives = 236/339 (69.62%), Query Frame = 2
Query: 2960 DANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDN--VGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
DA+T N V+ FL S +L L+ELF +EQ+T+DIL EMGH +LK++GV AYG RHKILKGI++ RST+ G NVN+ C TLLVDLLP D ++ +VEE+MQ++IR H+DN GG F RY I++V+++ N+KLW++Y R+QE+ EEN NER+LFHGSPFI AIV RGFDERHAYIGGMFGAGIYFAE+SSKSNQYVYG+GGG GC HKD+SCY+CPRQ+LLCRVALGK+FLQ + +KMAHAPPGHHSVVGRPS GL +AEYV+YRGEQ+YP +LITY I P
Sbjct: 887 DADTITN--------VSGFLSSQQLHHLIELFEREQITLDILAEMGHDDLKQVGVSAYGFRHKILKGIAQL-RSTT---------GIGNNVNL----C---------------------------------TLLVDLLPDDKEFVAVEEEMQATIREHRDNGQAGGYFTRYNIIRVQKVQNRKLWERYAHRRQEIAEENFLQSNERMLFHGSPFINAIVQRGFDERHAYIGGMFGAGIYFAEHSSKSNQYVYGIGGGIGCPSHKDKSCYVCPRQLLLCRVALGKSFLQYSAMKMAHAPPGHHSVVGRPSAGGLHFAEYVVYRGEQSYPEYLITYQIVKP 1170 HSP 3 Score: 252.677 bits (644), Expect = 3.291e-67 Identity = 179/491 (36.46%), Postives = 262/491 (53.36%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFA--SGFG-RKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTIL--TPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEE-INIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDL 1639
+FE L DAC+ D+ K L+ VN + TPLH A S G RK+++E L +G+ ++ K++ L PLH A H D ++VL+ G+ NA D TPLH A +A +LL AD N+ + +GLTA LA+D+ KL+ + LLEAA+ G +T+ I+ P +VNC DGR STPLH AAG+NR +VQ LLE GA+V+A DKGGLVPLHNACSYGH EV + L++ GA VN DLW++TPLHEAA+KG+ ++C LLL +GADP N +P +L + ++ ELL L + + G +V+R++ NC + + T +H NN E+LL GA +N +K G PLH A ++D+ LL++++ +N G T LH AA+ + +C +L+ +G++ + +G +L T D+
Sbjct: 326 AFEYKGHCLLDACRKCDVSRAKKLV-CAEIVNFVHPY-TGDTPLHLAVVSPDGKRKQLMELLTRKGSLLNEKNKAFLTPLHLAAELLHYDAMEVLLKQGAKVNALDSLGQTPLHRCARDEQA--VRLLLSYAADTNIVSLEGLTAAQLASDSVLKLLKNPPDSETHLLEAAKAGDLDTVRRIVLNNPISVNCRDLDGRHSTPLHFAAGFNRVPVVQFLLEHGAEVYAADKGGLVPLHNACSYGHYEVTELLVKHGANVNVSDLWKFTPLHEAAAKGKYDICKLLLKHGADPMKKNRDGATPADLVKESDHDVAELL---RGPSALLDAAKKGNLARVQRLV----TPESINCRDAQGRNS-----------TPLHLAAGYNNF-----ECAEYLLENGA--DVNAQDKGGLIPLHNASSYGHLDIAALLIKHKTVVNATDKWGFT---PLHEAAQKGRTQLCSLLLAHGADAYMKNQEGQTPIELATADDV 784
BLAST of Poly [ADP-ribose] polymerase vs. TrEMBL
Match: A0A1S3I014 (Poly [ADP-ribose] polymerase OS=Lingula unguis OX=7574 GN=LOC106159753 PE=4 SV=1) HSP 1 Score: 1293.49 bits (3346), Expect = 0.000e+0 Identity = 692/1254 (55.18%), Postives = 880/1254 (70.18%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDNVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N DL VK L++ +VN+KD GRKS+PLHFA+GFGRK++VE L+ GA+V +D+GGLIPLHNACSFGHA+VV++L+ G++ NA+D WNYTPLHEAA+KGK DVCIVLLQ AD +++N+DG TA DLA + + K+V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLLA+GADPT +NCH+KS ++ A T +L+E L E++G L R + +VK+ L +NF HP+TGD+ +H SS++ KRK + E L+R+GA +LN NK+ +PLH+A +++++ +L+++ ++ L+G+ G+ ALH A+ + +L+ YG+ +I + QGY A+QL T+ + + + +VP + + D +Q+LEA+KAGD+E+V +++ I S VNCRD DGRHSTPLHFAAG+NR++VVK LLE+GADVHAKDKGGLVPLHNACSYGH +V ELLIQ+ A VNV DLWKFTPLHEA+AKGKFEI KLLL++ ADP K+N+DGHTPLDLVKEGDQDV D+L D+ALLEAAKKG + +V+KL T NINCRD QGRNSTPLHLA+GYNN+E+AEFLLE GADVNAQDKGGLIPLHNASSYGH D+AALL+K+SANVN+ D+WG+TPLHEA+QK RTQLC+LLLAHGAD +KN EGQ+P DL +A DV+ LL++A+ + +A + V +SN + N +GC +R R +G+ + N +SVA FL SI LD L ++F +EQ+TMDIL+EM H ELK++G+ AYGHRHKILKGI K +Q + + H P G T+L+DL D +Y SVEEQMQS+IR HKDNVGGIF++Y I+K++++ N++LW++Y RK+E+ +EN NERLLFHGSPFI AIV++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKD+SCYIC RQ+LLCR LGK+FLQ + VKMAH+PPGHHSV+GRPS+ GL++AEYV+YRGEQAYP +LITY I P
Sbjct: 22 RDLFEACRNGDLNKVKKLVNTN-NVNAKDTAGRKSSPLHFAAGFGRKDVVEHLLGIGADVHARDDGGLIPLHNACSFGHAEVVQLLLRQGAEANARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPSIRNTDGKTALDLA-EQSAKIVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRTRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHFEVTELLLKHGANVNAMDLWQFTPLHEAASKSRLEVCSLLLAHGADPTLVNCHSKSAIDAAPTRDLQEKLLNEYRGHALLEGARQADLARVKKYLS----------------PDTVNFKHPYTGDSALHC--SSSSPFPKRKQVTEALIRKGA--NLNDKNKEFLTPLHMAADKSHIEIVDVLLKHGA-KVNALDGL-GQTALHRVAQQGNIQAARLLLTYGAEPSIVSLQGYTAAQLGTE---------------SIQKVLRDDSVVPMAEEAAIGASDFD-----------------------------------IQLLEAAKAGDMEVVKKLI----GIHSNTVNCRDLDGRHSTPLHFAAGYNRVAVVKFLLEHGADVHAKDKGGLVPLHNACSYGHLEVTELLIQHGACVNVADLWKFTPLHEASAKGKFEIVKLLLKHGADPVKKNRDGHTPLDLVKEGDQDVADILRGDAALLEAAKKGNLDRVKKLATPDNINCRDQQGRNSTPLHLAAGYNNVEVAEFLLEHGADVNAQDKGGLIPLHNASSYGHVDIAALLIKFSANVNAVDRWGFTPLHEASQKGRTQLCALLLAHGADPTMKNQEGQTPHDLTTAEDVKVLLQDAMPPALMPPTTTKAAVPTLAGPVGASNGLDNLVGAVGGIGISQPNTGDGCIDRGRIEGEKDLEN-------MSVASFLSSIGLDQLKDIFEREQITMDILVEMRHDELKDIGINAYGHRHKILKGIEKLPSGAAQPMPF---------------TPATHSNPQG-------------------------TILIDLTTDDAEYVSVEEQMQSTIREHKDNVGGIFDKYNIIKIQKIRNKRLWERYQHRKKEVADENHGHDNERLLFHGSPFINAIVHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDKSCYICHRQLLLCRATLGKSFLQFSAVKMAHSPPGHHSVIGRPSMGGLTFAEYVVYRGEQAYPEYLITYQIVKP 1150
BLAST of Poly [ADP-ribose] polymerase vs. TrEMBL
Match: K1QV17 (Poly [ADP-ribose] polymerase OS=Crassostrea gigas OX=29159 GN=CGI_10020506 PE=4 SV=1) HSP 1 Score: 1291.95 bits (3342), Expect = 0.000e+0 Identity = 701/1280 (54.77%), Postives = 882/1280 (68.91%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCSLE------------ISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDNVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLI---ENPTKCLEPSQNNS 4003
R LF+AC+N DL VK L++ ++VN+KD GRKS+PLHFA+GFGRK++VE L+ GA+V KD+GGLIPLHNACSFGHA+VV++L+ +G+D NA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG TA DLA D + K V TGEYKK ELLEAAR+G E+ +M++LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLLA+GADPT +NCH+KS +++A T EL+E L E KG L CR + +VK+ L +++NF HP++GDT +H +S KRK ++E L+R+GA +LN NK+ + LH+A + D++ +L+++ ++ L+G+ G+ ALH A+ + C +L++YG + +I + QGY A+QL T+ ++ + L E PPV T + +Q+LEA+KAGD+E+V +++ N VNCRD DGRHSTPLHFAAG+NR+ VV+ LL++GADVHAKDKGGLVPLHNACSYGH +V ELLI++ A VNV DLWKFTPLHEAAAKGKFEICKLLL++ ADP+K+N+DG+TPLDLVKEGDQDV DLL D+ALLEAAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNN+E+AEFLLE GADVNAQDKGGLIPLHNASSYGH D+AALL+K++ VN+ D+WG+TPLHEAAQK RTQLCSLLLAHGAD +KN EG +PLDL +A DV LL++A+ S+ + SSN +L ++ + S G + + C++E +SV+ FL S+ L+ LV +F KEQ++MDIL+EMGH ELKE+G+ AYGHRHKI+KG+ K S S SL P G T+LVDL D +Y SV+EQMQS++R HKDNVGG+FNRY I K++++ N++LW KY R++E+ EEN NERLLFHGSPFI AIV++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCYIC RQ++LCRV LGK+FLQ + +KMAHAPPGHHS++GRPS GL + EYV+YRGEQAYP +LIT+ I E+P P+ S
Sbjct: 23 RDLFEACRNGDLNKVKKLVN-HHNVNAKDTAGRKSSPLHFAAGFGRKDVVEHLLECGASVHAKDDGGLIPLHNACSFGHAEVVQLLLRSGADANARDNWNYTPLHEAAIKGKIDVCIVLLQHCADPNIRNTDGKTALDLA-DPSAKAVLTGEYKKDELLEAARSGNEDKLMSLLTPLNVNCHASDGRKSTPLHLAAGYNRTRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHFEVTEMLLKAGASVNAMDLWQFTPLHEAASKSRVEVCSLLLAHGADPTLVNCHSKSAIDVAPTRELQERLQQEFKGHSLLEGCRQADVARVKKYL------------------EVVNFKHPYSGDTALHCAAAS--PFPKRKQVVEALIRKGA--NLNDKNKEYVTALHIAADKAHYDVMDVLLKH-GAKVNALDGL-GQTALHRVAQQGNMQACRLLMSYGVDSSIVSLQGYTAAQLATE-NIQKMLKED---------------------------------------------PPVGGT------------DVDIQLLEAAKAGDMEVVKKLISQNPHS----VNCRDLDGRHSTPLHFAAGYNRVVVVEYLLQHGADVHAKDKGGLVPLHNACSYGHFEVTELLIKHGACVNVADLWKFTPLHEAAAKGKFEICKLLLKHGADPNKKNRDGNTPLDLVKEGDQDVADLLRGDAALLEAAKKGNLARVQKLASQENINCRDTQGRNSTPLHLAAGYNNVEVAEFLLENGADVNAQDKGGLIPLHNASSYGHVDIAALLIKFNTCVNAVDRWGFTPLHEAAQKGRTQLCSLLLAHGADPTMKNQEGHTPLDLATADDVHALLQDAMPPAVQPPSLTKSSINLGNTSDASSNNSLSFNASDLGVAAGTSGTSAA--------GASMSMGAEGCNMERTMAEGERGIDSMSVSAFLNSVGLEQLVCVFEKEQISMDILVEMGHDELKEVGINAYGHRHKIMKGLEKLAASKV--------------------SASLPAVPTQG------------------------TVLVDLTTDDPEYISVDEQMQSTLREHKDNVGGLFNRYNISKIQKISNKRLWGKYCHRRKEVAEENHGHDNERLLFHGSPFINAIVHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPAHKDRSCYICHRQLILCRVTLGKSFLQFSAIKMAHAPPGHHSIIGRPSSGGLMFPEYVVYRGEQAYPEYLITFQICKPESPDNSAAPTPKKS 1162 HSP 2 Score: 308.145 bits (788), Expect = 4.943e-83 Identity = 158/326 (48.47%), Postives = 220/326 (67.48%), Query Frame = 2
Query: 1808 STMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEE 2785
S ++ ++++ + + EA + GD+ V +++ +++ VN +DT GR S+PLHFAAGF R VV+ LLE GA VHAKD GGL+PLHNACS+GHA+V +LL++ A+ N D W +TPLHEAA KGK ++C +LLQ+ ADP+ RN DG T LDL + V LLEAA+ G K+ L+T +N+NC + GR STPLHLA+GYN I + LL+ GADV+A+DKGGL+PLHNA SYGH ++ +LLK A+VN+ D W +TPLHEAA K+R ++CSLLLAHGAD L N +S +D+ +++ L++
Sbjct: 6 SLLQNESSSPSHANDPGRDLFEACRNGDLNKVKKLVNHHN------VNAKDTAGRKSSPLHFAAGFGRKDVVEHLLECGASVHAKDDGGLIPLHNACSFGHAEVVQLLLRSGADANARDNWNYTPLHEAAIKGKIDVCIVLLQHCADPNIRNTDGKTALDLADPSAKAVLTGEYKKDELLEAARSGNEDKLMSLLTPLNVNCHASDGRKSTPLHLAAGYNRTRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHFEVTEMLLKAGASVNAMDLWQFTPLHEAASKSRVEVCSLLLAHGADPTLVNCHSKSAIDVAPTRELQERLQQ 325
BLAST of Poly [ADP-ribose] polymerase vs. TrEMBL
Match: A0A1S3I021 (Poly [ADP-ribose] polymerase OS=Lingula unguis OX=7574 GN=LOC106159753 PE=4 SV=1) HSP 1 Score: 1291.95 bits (3342), Expect = 0.000e+0 Identity = 690/1254 (55.02%), Postives = 880/1254 (70.18%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDNVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N DL VK L++ +VN+KD GRKS+PLHFA+GFGRK++VE L+ GA+V +D+GGLIPLHNACSFGHA+VV++L+ G++ NA+D WNYTPLHEAA+KGK DVCIVLLQ AD +++N+DG TA DLA + + K+V TGEYKK ELLE+AR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLLA+GADPT +NCH+KS ++ A T +L+E L E++G L R + +VK+ L +NF HP+TGD+ +H SS++ KRK + E L+R+GA +LN NK+ +PLH+A +++++ +L+++ ++ L+G+ G+ ALH A+ + +L+ YG+ +I + QGY A+QL T+ + + + +VP + + D +Q+LEA+KAGD+E+V +++ I S VNCRD DGRHSTPLHFAAG+NR++VVK LLE+GADVHAKDKGGLVPLHNACSYGH +V ELLIQ+ A VNV DLWKFTPLHEA+AKGKFEI KLLL++ ADP K+N+DGHTPLDLVKEGDQDV D+L D+ALLEAAKKG + +V+KL T NINCRD QGRNSTPLHLA+GYNN+E+AEFLLE GADVNAQDKGGLIPLHNASSYGH D+AALL+K+SANVN+ D+WG+TPLHEA+QK RTQLC+LLLAHGAD +KN EGQ+P DL +A DV+ LL++A+ + +A + V +SN + N +GC +R R +G+ + N +SVA FL SI LD L ++F +EQ+TMDIL+EM H ELK++G+ AYGHRHKILKGI K +Q + + H P G T+L+DL D +Y SVEEQMQS+IR HKDNVGGIF++Y I+K++++ N++LW++Y RK+E+ +EN NERLLFHGSPFI AIV++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKD+SCYIC RQ++LCRV LGK+FLQ + +KMAH+PPGHHSV+GRPS+ L++AEYV+YRGEQAYP +LITY I P
Sbjct: 22 RDLFEACRNGDLNKVKKLVNTN-NVNAKDTAGRKSSPLHFAAGFGRKDVVEHLLGIGADVHARDDGGLIPLHNACSFGHAEVVQLLLRQGAEANARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPSIRNTDGKTALDLA-EQSAKIVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRTRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHFEVTELLLKHGANVNAMDLWQFTPLHEAASKSRLEVCSLLLAHGADPTLVNCHSKSAIDAAPTRDLQEKLLNEYRGHALLEGARQADLARVKKYLS----------------PDTVNFKHPYTGDSALHC--SSSSPFPKRKQVTEALIRKGA--NLNDKNKEFLTPLHMAADKSHIEIVDVLLKHGA-KVNALDGL-GQTALHRVAQQGNIQAARLLLTYGAEPSIVSLQGYTAAQLGTE---------------SIQKVLRDDSVVPMAEEAAIGASDFD-----------------------------------IQLLEAAKAGDMEVVKKLI----GIHSNTVNCRDLDGRHSTPLHFAAGYNRVAVVKFLLEHGADVHAKDKGGLVPLHNACSYGHLEVTELLIQHGACVNVADLWKFTPLHEASAKGKFEIVKLLLKHGADPVKKNRDGHTPLDLVKEGDQDVADILRGDAALLEAAKKGNLDRVKKLATPDNINCRDQQGRNSTPLHLAAGYNNVEVAEFLLEHGADVNAQDKGGLIPLHNASSYGHVDIAALLIKFSANVNAVDRWGFTPLHEASQKGRTQLCALLLAHGADPTMKNQEGQTPHDLTTAEDVKVLLQDAMPPALMPPTTTKAAVPTLAGPVGASNGLDNLVGAVGGIGISQPNTGDGCIDRGRIEGEKDLEN-------MSVASFLSSIGLDQLKDIFEREQITMDILVEMRHDELKDIGINAYGHRHKILKGIEKLPSGAAQPMPF---------------TPATHSNPQG-------------------------TILIDLTTDDAEYVSVEEQMQSTIREHKDNVGGIFDKYNIIKIQKIRNKRLWERYQHRKKEVADENHGHDNERLLFHGSPFINAIVHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDKSCYICHRQLVLCRVTLGKSFLQFSAIKMAHSPPGHHSVIGRPSMGALTFAEYVVYRGEQAYPEYLITYQIVKP 1150
BLAST of Poly [ADP-ribose] polymerase vs. TrEMBL
Match: A0A2T7P7U8 (Poly [ADP-ribose] polymerase OS=Pomacea canaliculata OX=400727 GN=C0Q70_08742 PE=4 SV=1) HSP 1 Score: 1290.79 bits (3339), Expect = 0.000e+0 Identity = 704/1271 (55.39%), Postives = 875/1271 (68.84%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDNVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQNNSENMDNL 4021
R LF+AC+N DL VK L+ ++VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV++L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG TA DLA D++ K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADP +NCH+KS +++A T EL+E + FE+KG L R + +VK+ L + ++NF HP+ GD+ +H +S KRK ++E L+R+GA +LN NK + LH+A + D++ +L+++ ++ L+ + G+ ALH A+ + C +L++Y + TI ++QGY A+QL T+ ++ + L E PPV T + +Q+LEA+KAGD++LV +++ N VNCRD DGRHSTPLHFAAG+NR++VV+ LLE+GADVHAKDKGGLVPLHNACSYGH +V ELLI++ A VNV DLWKFTPLHEAA+KGKFEICKLLL++ ADP+K+N+DGHTPLDLVKEGDQDV DLL D+ALLEAAKKG +A+VQKL ++ NINCRDTQGRNSTPLHLA+GYNN+E+AEFLLE GADVNAQDKGGLIPLHNASSYGH D+AALL+KY+ VN+ D+WG+TPLHEAAQK RTQLC+LLLAHGAD +KN EG +PLDL SA DV+ LL +A+ + SSN +L ++ G GD + + +SVA L S+ L+ L ++F +EQ+TMDIL+EMGH ELK++G+ AYGHRHKILKG+ K SC G V + S T+L+DL P D + SV +QMQS++R HKDNVGGIFNRY ILKV+++ N+KL +KYL R++E+ EEN NE LLFHGSPFI AIV++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQ+LLCRV LGK+FLQ + +KMAHAPPGHHSV GRPS GL+Y EYVIYRGEQAYP FLIT+ I P EP +N + L
Sbjct: 23 RELFEACRNGDLNKVKKLVS-SHNVNAKDTAGRKSTPLHFAAGFGRKDVVEHLLENGANVHARDDGGLIPLHNACSFGHAEVVQLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKTALDLA-DSSAKAVLTGEYKKDELLEAARSGNEEQMMALLTPLNVNCHASDGRKSTPLHLAAGYNRTRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHFEVTEMLLKAGASVNAVDLWQFTPLHEAASKSRVEVCSLLLSHGADPNLVNCHSKSIIDVAPTRELQERIQFEYKGHSLLEGARQADLARVKKYLSV----------------DVVNFKHPYNGDSALHCAAASP--FPKRKQVVELLIRKGA--NLNDKNKDYLTALHIAADKAHYDVMDVLLKH-GAKVNGLDSL-GQTALHRVAQQGNMQACRLLMSYAVDPTIVSHQGYTAAQLATE-NIQKLLQED---------------------------------------------PPVGGT------------DIDLQLLEAAKAGDLDLVKKLVTTNVHA----VNCRDVDGRHSTPLHFAAGYNRVAVVEYLLEHGADVHAKDKGGLVPLHNACSYGHYEVTELLIKHGACVNVADLWKFTPLHEAASKGKFEICKLLLKHGADPNKKNRDGHTPLDLVKEGDQDVADLLRGDAALLEAAKKGNLARVQKLASTENINCRDTQGRNSTPLHLAAGYNNVEVAEFLLENGADVNAQDKGGLIPLHNASSYGHVDIAALLIKYNTCVNAQDRWGFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGHTPLDLASADDVKALLADAMPLLTLPPSLPKGQLGSLASSTSSSNNSLFNSSTEAVMGAVGGTAQVGDG----CAGDHSVAEGERSIESLSVAGLLASLGLEQLRDIFEQEQITMDILMEMGHEELKDIGINAYGHRHKILKGVEKL-------VSCR---GIRTVVGMASQG----------------------------------TVLLDLGPDDPELISVSDQMQSTLRDHKDNVGGIFNRYDILKVQKIRNKKLLEKYLHRRKEVSEENHGQPNEHLLFHGSPFISAIVHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICNRQLLLCRVTLGKSFLQFSAMKMAHAPPGHHSVTGRPSQGGLNYTEYVIYRGEQAYPEFLITFKIIRP----EPVENKNATHKEL 1155 HSP 2 Score: 311.997 bits (798), Expect = 6.086e-83 Identity = 159/317 (50.16%), Postives = 218/317 (68.77%), Query Frame = 2
Query: 1829 TNDKQVEEY---CMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVR 2770
TND V + ++ EA + GD+ V +++ +++ VN +DT GR STPLHFAAGF R VV+ LLENGA+VHA+D GGL+PLHNACS+GHA+V +LL+++ A+ N D W +TPLHEAA KGK ++C +LLQ+ ADP+ RN DG T LDL + V LLEAA+ G ++ L+T +N+NC + GR STPLHLA+GYN I + LL+ GADV+A+DKGGL+PLHNA SYGH ++ +LLK A+VN+ D W +TPLHEAA K+R ++CSLLL+HGAD L N +S +D+ +++
Sbjct: 10 TNDTSVPSHNDPGRELFEACRNGDLNKVKKLVSSHN------VNAKDTAGRKSTPLHFAAGFGRKDVVEHLLENGANVHARDDGGLIPLHNACSFGHAEVVQLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKTALDLADSSAKAVLTGEYKKDELLEAARSGNEEQMMALLTPLNVNCHASDGRKSTPLHLAAGYNRTRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHFEVTEMLLKAGASVNAVDLWQFTPLHEAASKSRVEVCSLLLSHGADPNLVNCHSKSIIDVAPTRELQ 320 HSP 3 Score: 265.003 bits (676), Expect = 4.907e-68 Identity = 186/501 (37.13%), Postives = 261/501 (52.10%), Query Frame = 2
Query: 194 FEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLV-----FTGEYKKQELLEAARNGLEETIMTILTP--FNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSE 1657
FE +L + + DL VK L + VN K + S LH A+ RK++VE LI +GAN++ K++ L LH A H DV+ VL+ G+ N D T LH A +G C +L+ D + + G TA LAT+N KL+ G +LLEAA+ G + + ++T VNC DGR STPLH AAGYNR +V+ LLE GA VHAKDKGGLVPLHNACSYGH EV + L++ GA VN DLW++TPLHEAASKG+ E+C LLL +GADP N +PL+L ++ +LL + L + G +V Q L +T + NC + + T +H NNV + EFLL GA +N +K G PLH A +VD+ LL++ N +N G T LH AA+ + +C +L+ +G++ T+ +G+ L + D+ L++
Sbjct: 325 FEYKGHSLLEGARQADLARVKKYLSVDV-VNFKHPYNGDSA-LHCAAASPFPKRKQVVELLIRKGANLNDKNKDYLTALHIAADKAHYDVMDVLLKHGAKVNGLDSLGQTALHRVAQQGNMQACRLLMSYAVDPTIVSHQGYTAAQLATENIQKLLQEDPPVGGTDIDLQLLEAAKAGDLDLVKKLVTTNVHAVNCRDVDGRHSTPLHFAAGYNRVAVVEYLLEHGADVHAKDKGGLVPLHNACSYGHYEVTELLIKHGACVNVADLWKFTPLHEAASKGKFEICKLLLKHGADPNKKNRDGHTPLDLVKEGDQDVADLLRGD---AALLEAAKKGNLARV----QKLASTENINCRDTQGRN-----------STPLHLAAGYNNV-----EVAEFLLENGA--DVNAQDKGGLIPLHNASSYGHVDIAALLIKYNTCVNAQDRWGFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGHTPLDLASADDVKALLAD 795
BLAST of Poly [ADP-ribose] polymerase vs. TrEMBL
Match: A0A087UUU1 (Poly [ADP-ribose] polymerase (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_08871 PE=4 SV=1) HSP 1 Score: 1278.46 bits (3307), Expect = 0.000e+0 Identity = 694/1273 (54.52%), Postives = 879/1273 (69.05%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAI-SQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLX-------------XXXXXXXXXXXXXXXXXRLRTDG--DANTSNNNNCSLE-ISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDN--VGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LFDAC+ D+ V+ L+ +VN++D GRKSTPLHFA+GFGR+++VE L+ GANV +D+GGLIPLHNACSFGHA+VV++L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD ++N+DG TA DLA D + + V TGEY+K ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + L++ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH+KS +++A+ +L E L +E+KG L RL + +VK+ L + +INF HPFTGDT +H ++SS KRK++ E +R+GA SLN NK+ +PLH+A + D++ L+++ ++ L+G+ G+ ALH A + C IL++Y + +I + QGY A+Q+ + + + LSE PT I +++ K+ GD+E+V RIL + + VNCRD DGR STPLHFAAG+NR+SVV+ LL++GADVHAKDKGGLVPLHNACSYGH +VAELL++Y ANVNV DLWKFTPLHEAAAKGK+EI KLLL++ ADP K+N+DGHTPLDLVKEGDQDV DLL D+ALL+AAKKG +++V KLVT NINCRD+QGRNSTPLHLA+GYNNLE+AEFLLE GADVNAQDKGGLIPLHNASSYGH D+AALL+KY+ VN+ D+WG+TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+P DL +A DV+ LL +A+ + S+ P++ SS+ + P+ + + ++ + S DG D + + + ++E I ++ FL ++ LD L ++F KEQ+T+DIL EMGH ELK++GV AYGHRHK+LKGI K C H+ S + KPG TLLV+L P D +YQ+V+E+MQ++IR H+DN GG+F RY ILK++++ N+KLW+KY R++E+ EEN + NER+LFHGSPFI AIV +GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCY+C RQ+LLCRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GLSY EYV+YRGEQAYP +LITY I P
Sbjct: 23 RELFDACRYGDVSRVRKLV-TPQNVNARDAAGRKSTPLHFAAGFGRRDVVEHLLQNGANVHARDDGGLIPLHNACSFGHAEVVQLLLKHGADPNARDNWNYTPLHEAAIKGKTDVCIVLLQHGADPAIRNTDGKTALDLA-DPSARAVLTGEYRKDELLEAARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRIRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHFEVTEILIKHGANVNAMDLWQFTPLHEAASKARVEVCSLLLSHGADPTLLNCHSKSAIDVASARDLPERLSYEYKGHCLLEAARLADPARVKKYLSL----------------DVINFKHPFTGDTPLHCSVSSP--YPKRKHVTEVFIRKGA--SLNEKNKEYLTPLHVAADKSHFDVMDALLKHGA-KVNALDGL-GQTALHRCAREGNVQACRILLSYDIDTSIISLQGYTAAQVANE-SVQKLLSE-----PTTVGIDIEYQLLEAAKA-----------------------------------------------------GDMEVVKRILATHLPV----VNCRDVDGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKYGANVNVADLWKFTPLHEAAAKGKYEIVKLLLKHGADPTKKNRDGHTPLDLVKEGDQDVADLLRGDAALLDAAKKGNLSRVLKLVTPENINCRDSQGRNSTPLHLAAGYNNLEVAEFLLEHGADVNAQDKGGLIPLHNASSYGHLDIAALLIKYNTVVNATDRWGFTPLHEAAQKGRTQLCALLLAHGADPTVKNHEGQTPFDLATAEDVKCLLVDAMPPPSLPPSTKSSTVGTSVIPVIASSSISTPSAIANFPVIGLEDDIPLSLNLLSSQKMPNVSVSGSQHGDGCMDFDRAEAPDPTMENIGISGFLSNLGLDHLADIFEKEQITLDILAEMGHEELKQIGVNAYGHRHKLLKGIEKL---------CTG----------HNASTFMPNKPG--------------------------TLLVELGPEDKEYQAVDEEMQATIREHRDNGHAGGVFARYNILKIQKVRNKKLWEKYCHRRKEVAEENHSQSNERMLFHGSPFINAIVQKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYVCHRQLLLCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVGGLSYPEYVVYRGEQAYPEYLITYQIAKP 1163 HSP 2 Score: 315.464 bits (807), Expect = 1.925e-85 Identity = 158/314 (50.32%), Postives = 216/314 (68.79%), Query Frame = 2
Query: 1826 TTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADV 2767
+++ + + ++ +A + GDV V +++ + VN RD GR STPLHFAAGF R VV+ LL+NGA+VHA+D GGL+PLHNACS+GHA+V +LL+++ A+ N D W +TPLHEAA KGK ++C +LLQ+ ADP RN DG T LDL + V LLEAA+ G K+ L+T +N+NC + GR STPLHLA+GYN + I + LL+ GADV+A+DKGGL+PLHNA SYGH ++ +L+K+ ANVN+ D W +TPLHEAA K R ++CSLLL+HGAD L N +S +D+ SA D+
Sbjct: 12 ASDNSLISDIGRELFDACRYGDVSRVRKLVTPQN------VNARDAAGRKSTPLHFAAGFGRRDVVEHLLQNGANVHARDDGGLIPLHNACSFGHAEVVQLLLKHGADPNARDNWNYTPLHEAAIKGKTDVCIVLLQHGADPAIRNTDGKTALDLADPSARAVLTGEYRKDELLEAARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRIRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHFEVTEILIKHGANVNAMDLWQFTPLHEAASKARVEVCSLLLSHGADPTLLNCHSKSAIDVASARDL 319
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Cavefish
Match: tnksb (tankyrase-1 [Source:NCBI gene;Acc:103045612]) HSP 1 Score: 1255.74 bits (3248), Expect = 0.000e+0 Identity = 675/1267 (53.28%), Postives = 878/1267 (69.30%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXX-------XXXXXXXXXXXXRLRTDGDANTSNNNN--CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G E+ +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH+KS ++LA T ELR+ L +E KG L R G+ KVK+ Q++I+F HP + D+ +H ++S + KRK + E LLR+GA +++ NK +P H+A + D++++L ++ ++N +D L G+ ALH AA C +L++YG++ +I + QG+ A+Q+ + + + LS +E + P GE P S + + LEA+KAGD++ V ++ + VNCRD +GRHSTPLHFAAG+NR++VV+ LL +GADVHAKDKGGLVPLHNACSYGH +VAELL+++ A+VNV DLWKFTPLHEAAAKGK+EICKLLL++ ADP K+N+DG+ LD+VK+GD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+K++ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+ LDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + ++ DG + T L++++++FL+S+ L+ L ++F +EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KGI + G +P + H + T+L+DL P D ++QSVEE+MQS+IR H+D N GG+F+RY ++K++++ N+KL ++Y R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCY+C RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 82 RELFEACRNGDVSRVKRLVDAA-NVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKTVLTGEYKKDELLEAARSGNEDKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHSKSAIDLAPTPELRDKLTYEFKGHSLLQAAREGDLAKVKKT-----------------PQEIISFKHPHSHDSALHCVVASPH--PKRKQVTELLLRKGA--NIHDKNKDFMTPFHVAAERAHNDVLEVLQKHGAKVNAVDTL----GQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTAAQMGNEA-VQQILSGEESVIPV--------------------------------HMAGENVPARNSDVD-------------YRFLEAAKAGDLDTVKQLCSPQN------VNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPDNINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSATLI--SPASTPSCLSAASSIDNLAGPLSDGATGGASGGATGPADGASGTERKEGEVTMLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERL---------LGGQQGGNPYLAFHCTA--------------------------------QGTVLIDLPPDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYVCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIAKP 1226
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Cavefish
Match: tnksb (tankyrase-1 [Source:NCBI gene;Acc:103045612]) HSP 1 Score: 1249.19 bits (3231), Expect = 0.000e+0 Identity = 675/1274 (52.98%), Postives = 878/1274 (68.92%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRK-------STPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXX-------XXXXXXXXXXXXRLRTDGDANTSNNNN--CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G E+ +M +LTP NVNCHASDGRK STPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH+KS ++LA T ELR+ L +E KG L R G+ KVK+ Q++I+F HP + D+ +H ++S + KRK + E LLR+GA +++ NK +P H+A + D++++L ++ ++N +D L G+ ALH AA C +L++YG++ +I + QG+ A+Q+ + + + LS +E + P GE P S + + LEA+KAGD++ V ++ + VNCRD +GRHSTPLHFAAG+NR++VV+ LL +GADVHAKDKGGLVPLHNACSYGH +VAELL+++ A+VNV DLWKFTPLHEAAAKGK+EICKLLL++ ADP K+N+DG+ LD+VK+GD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+K++ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+ LDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + ++ DG + T L++++++FL+S+ L+ L ++F +EQ+T+D+L +MGH ELKE+G+ AYGHRHK++KGI + G +P + H + T+L+DL P D ++QSVEE+MQS+IR H+D N GG+F+RY ++K++++ N+KL ++Y R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCY+C RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 82 RELFEACRNGDVSRVKRLVDAA-NVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHSRDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKTVLTGEYKKDELLEAARSGNEDKLMALLTPLNVNCHASDGRKSTSQKMLSTPLHLAAGYNRVRIVQLLLQYGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHSKSAIDLAPTPELRDKLTYEFKGHSLLQAAREGDLAKVKKT-----------------PQEIISFKHPHSHDSALHCVVASPH--PKRKQVTELLLRKGA--NIHDKNKDFMTPFHVAAERAHNDVLEVLQKHGAKVNAVDTL----GQTALHRAALAGHIQTCRLLLSYGADPSIVSLQGFTAAQMGNEA-VQQILSGEESVIPV--------------------------------HMAGENVPARNSDVD-------------YRFLEAAKAGDLDTVKQLCSPQN------VNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPSKKNRDGNMALDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPDNINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKFNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVLSATLI--SPASTPSCLSAASSIDNLAGPLSDGATGGASGGATGPADGASGTERKEGEVTMLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGIERL---------LGGQQGGNPYLAFHCTA--------------------------------QGTVLIDLPPDDKEFQSVEEEMQSTIREHRDGGNAGGVFSRYNVIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYVCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIAKP 1233
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Cavefish
Match: ENSAMXT00000005100.2 (tankyrase-2 [Source:NCBI gene;Acc:103036789]) HSP 1 Score: 590.497 bits (1521), Expect = 0.000e+0 Identity = 356/823 (43.26%), Postives = 497/823 (60.39%), Query Frame = 2
Query: 179 LTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNE-EINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNK-TDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTP 2641
+ A+ E R LF+AC++ DL+ V+ LL+ +VNS+D GRKSTPLHFA+GFGR+++V+ L+ GA+V +D+GGL+ LHNACSFGH +VV L+ G+D N++D WNYTPLHEAA+KGK DVCIVLLQ A++ ++N+DG TA DLA + TK V TGEY+K ELLE+AR+G EE +M++LTP NVNCHASDGRKSTPLHLAAGYNR R VQLLL+ GA VHAKDKG LVPLHNACSYGH EV + L++ GA VNA DLW++TPLHEAASK R EVCSLLL++GADPT LNCHNKS ++LA T L+E L +E +G L R ++ ++K+ L + + INF HP T +T +H S ++ KR + E LLR GA ++N + +PLHLA + D+I++L++ E ++ + ++ G+ L A+ C +L+ G ++ I + QG+ +QL ++ E L E + C D + + + L T K N EG+ + P+ AS V +V +L++ +D V+ +D G PLH A + V ++L+ +GA ++ D +PLH A + G ++ +L LLQ+ ADP K+N+DG+TPLDLVKEGD D+ DLL + LL+AA+KG +A++ K+ N+NCR++QGR+ TP+HL +GY N E AE+LL+ GA+ N+QDK LI LHNA+SY D+ ALL +A SA +TP
Sbjct: 19 MERAAGGEPNRRLFEACRSGDLERVRKLLNAA-NVNSRDTAGRKSTPLHFAAGFGRRDVVDHLLKHGADVHARDDGGLVSLHNACSFGHVEVVSHLLRHGADANSRDNWNYTPLHEAAIKGKIDVCIVLLQHGAELTIRNTDGRTALDLA-EPFTKAVLTGEYRKDELLESARSGNEEKLMSLLTPLNVNCHASDGRKSTPLHLAAGYNRVRTVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAVDLWRFTPLHEAASKNRVEVCSLLLSHGADPTFLNCHNKSAIDLAPTTLLKERLAYEFRGHALLKAIRETDFIRIKKYLSL----------------ETINFKHPQTHETALHCAASLSS--PKRTQICEMLLRNGA--NINEKTRDLLTPLHLAAEKSHKDVIEVLMKYEAKVPVKTVDS-QGQAPLQRVAQCGHLQNCRLLIGAGCDQPITSLQGFYPAQLANQ-NIQENLQEGVLM---GNCDKDRHLLEASKSGDLETVKKLCTLQNVNCRDLEGQQSTPLHF---------------------ASGYNRVTVVDYLLQHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVIHGAVINVADLWKFMPLHEAAAKGKYEICKL---------------------------------LLQHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGEDELLDAARKGCLARISKVCRPDNVNCRNSQGRHCTPIHLPAGYKNFEAAEYLLQHGAEENSQDKSRLISLHNAASYRVNDVRALLT--AAMPPSALPTCFTP 751 HSP 2 Score: 508.834 bits (1309), Expect = 5.360e-159 Identity = 414/1203 (34.41%), Postives = 588/1203 (48.88%), Query Frame = 2
Query: 674 QELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEE-INIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAK--DKGGLVPLHNACSYGH------------------------AKVAELLIQYN----------------------------------ANVNVCDL--WKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPL-DLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALL--------------------LKYSANVNSADKWGY--TPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPL-DLCSAA-----DVRFLLEEAISQTXX------XXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANT----SNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDN--VGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
+ L EA R+G E + +L NVN + GRKSTPLH AAG+ R +V LL+ GA VHA+D GGLV LHNACS+GH+EV LL GA N+ D W YTPLHEAA KG+ +VC +LL +GA+ T N ++ L+LA + +L E++ L R G K+ +L L + NC ++ T +H N V + ++ LL+ GA ++ +K PLH A + ++ +LLV++ +N L T LH AA N +C +L+++G++ T A L L E L+ + I +++ I + K+ S E T+ K + +C L + K ++ +L N ++I RD TPLH AA + V++VL++ A V K D G PL GH A++A IQ N NVN DL + TPLH A+ + + LLQ+ AD H ++K G PL + G +V +LL ++ IN D PLH A+ EI + LL+ GAD +++ G PL + G D+ LL + NVN + G TP+H A + LL HGA+ +N + +S L L +AA DVR LL A+ +A V S+S++A S + S++ T P++ ++ L T GD + ++S+++FL+ + L L+E+F +EQ+T+D+L+EMGH ELKE+G+ AYGHRHKI++G+ + AP N P + +++ + T+L+DL + ++QSVEE+MQS++R H+D GGIFN Y +LK++++CN+KLW+KY R++E+ EEN N NER+LFHGSPF+ +I+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC LHKDRSCY C RQ+L CRV LGK+FLQ + +KMAH+PPGHHSV GRPSV GL+ AEYVIYRGEQAYP +LITY I P
Sbjct: 29 RRLFEACRSGDLERVRKLLNAANVNSRDTAGRKSTPLHFAAGFGRRDVVDHLLKHGADVHARDDGGLVSLHNACSFGHVEVVSHLLRHGADANSRDNWNYTPLHEAAIKGKIDVCIVLLQHGAELTIRNTDGRTALDLAEPFT-KAVLTGEYRKDELLESARSGNEEKLMSLLTPL----NVNCHASDGRKS-----------TPLHLAAGYNRVRT-----VQLLLQHGA--DVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAVDLWRFT---PLHEAASKNRVEVCSLLLSHGADPTFLNCHNKSAIDLAPTTLLKERLAYEFRGHALLKAIRETDFI------------RIKKYLSLE-------------TINFKHPQTHETALHCAASLSSPKR--TQICEMLLRNGANINE---KTRDL----LTPLHLAAEKSHKDVIEVLMKYEAKVPVKTVDSQGQAPLQRVAQCGHLQNCRLLIGAGCDQPITSLQGFYPAQLANQNIQENLQEGVLMGNCDKDRHLLEASKSGDLETVKKLCTLQNVNCRDLEGQQSTPLHFASGYNRVTVVDYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELL--------------------VIHGAVINVADLW--KFMPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPL-DLVKEGDTDIQDLLRGEDELLDAARKGCLARISKVCRPDNVNCRNSQGRHCTPIHLPAGYKNFEAAEYLLQHGAE---ENSQDKSRLISLHNAASYRVNDVRALLTAAMPPSALPTCFTPQVFSVSASAEAVSSSLASNSSTHPSR-----------SSMEAFHKLLGTSGDEGAMRVEKTEEGVAPDMSISQFLQKLGLKYLLEIFDREQITLDVLVEMGHKELKEIGISAYGHRHKIIRGVERL----ISAPQSLN-----PYLTLNTANSG--------------------------------TILIDLALDEKEFQSVEEEMQSTVREHRDGGFAGGIFNTYRVLKIQKVCNKKLWEKYTHRRKEVSEENHNHCNERMLFHGSPFVNSIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPLHKDRSCYTCERQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVSGRPSVDGLALAEYVIYRGEQAYPEYLITYQIIKP 1093 HSP 3 Score: 206.068 bits (523), Expect = 1.581e-53 Identity = 138/371 (37.20%), Postives = 200/371 (53.91%), Query Frame = 2
Query: 155 IADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFG---RKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAK--DVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEY-----KKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSN 1231
+A T+L K ++E L A + D +K L + +N K + T LH A+ R ++ E L+ GAN++ K L PLH A H DV++VL+ + K D PL A G C +L+ A D + + G LA N + + G K + LLEA+++G ET+ + T NVNC +G++STPLH A+GYNR +V LL+ GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA++N DLW++ PLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL E + L + R G ++ ++ + ++ NC N
Sbjct: 319 LAPTTLLK-ERLAYEFRGHALLKAIRETDFIRIKKYLSL-ETINFK-HPQTHETALHCAASLSSPKRTQICEMLLRNGANINEKTRDLLTPLHLAAEKSHKDVIEVLMKYEAKVPVKTVDSQGQAPLQRVAQCGHLQNCRLLIGAGCDQPITSLQGFYPAQLANQNIQENLQEGVLMGNCDKDRHLLEASKSGDLETVKKLCTLQNVNCRDLEGQQSTPLHFASGYNRVTVVDYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVIHGAVINVADLWKFMPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGEDE---LLDAARKGCLARISKVCR----PDNVNCRN 679 HSP 4 Score: 53.5286 bits (127), Expect = 1.959e-6 Identity = 38/116 (32.76%), Postives = 58/116 (50.00%), Query Frame = 2
Query: 152 KIADTSLSKLTEASFEENCRTLFDACKNNDLQVVK--CLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNA 493
K DT + L E L DA + L + C D +VN +++ GR TP+H +G+ E E+L+ GA + +D+ LI LHNA S+ D V+ L++A P+A
Sbjct: 638 KEGDTDIQDLLRGEDE-----LLDAARKGCLARISKVCRPD---NVNCRNSQGRHCTPIHLPAGYKNFEAAEYLLQHGAEENSQDKSRLISLHNAASYRVND-VRALLTAAMPPSA 744
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Cavefish
Match: tnksa (tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase a [Source:ZFIN;Acc:ZDB-GENE-030131-7450]) HSP 1 Score: 495.738 bits (1275), Expect = 1.178e-160 Identity = 249/419 (59.43%), Postives = 311/419 (74.22%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN 1465
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPN++D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA D + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT +NCH KS +++A T EL+E L +E KG L R + KVK+ L + ++INF HP T +T +H ++S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++
Sbjct: 128 RELFEACRNGDVSRVKRLVD-SVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNSRDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTLAL----------------EIINFKHPQTHETALHCAVASPH--PKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDILEVLQKH 524 HSP 2 Score: 314.309 bits (804), Expect = 2.061e-93 Identity = 159/303 (52.48%), Postives = 210/303 (69.31%), Query Frame = 2
Query: 1862 QILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVR 2770
++ EA + GDV V R+++ SV VN +D GR STPLHFAAGF R VV+ LL+ GA+VHA+D GGL+PLHNACS+GHA+V LL+ A+ N D W +TPLHEAA KGK ++C +LLQ+ ADP+ RN DG + LDL + V LLEAA+ G K+ L+T +N+NC + GR STPLHLA+GYN + I + LL+ GADV+A+DKGGL+PLHNA SYGH ++ LLLK+ A VN+ D W +TPLHEAA KNR ++CSLLL+HGAD L N G+S +D+ +++
Sbjct: 129 ELFEACRNGDVSRVKRLVD------SVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNSRDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELK 425 HSP 3 Score: 201.83 bits (512), Expect = 1.285e-54 Identity = 157/499 (31.46%), Postives = 243/499 (48.70%), Query Frame = 2
Query: 1142 LFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-----RNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGD-QDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGY---NNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANV 2611
LF CR G+ ++VKR++ + N N + + K ++F F RK+++E LL+ GA +++ + G PLH A + +++ LL+ N N + LH AA +C +L+ +G++ I G A +DL + P+ K+ L K D ++LEA+++G+ E + +L + VNC +DGR STPLH AAG+NR+ +V++LL++GADVHAKDKGGLVPLHNACSYGH +V ELL+++ A VN DLW+FTPLHEAA+K + E+C LLL + ADP N G + +D+ + ++ +LL+AA++ +AKV+K + IN + Q + T LH A ++ E LL +GA+VN ++K + PLH A+ H D+ +L K+ A V
Sbjct: 130 LFEACRNGDVSRVKRLVDSV-NVNAKDMAG--RKSTPLHFAAGFG-----------------RKDVVEHLLQTGA--NVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNSRDNWNY-------TPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSA------LDLAD----------------------PSAKAVLTGEYKKD------------------------------------ELLEAARSGNEEKLMALL------TPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTLALEIINFKHPQ-THETALHCAVASPHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDILEVLQKHGAKV 528 HSP 4 Score: 167.548 bits (423), Expect = 4.928e-43 Identity = 109/305 (35.74%), Postives = 164/305 (53.77%), Query Frame = 2
Query: 110 NPNEYCTDIQDICDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGY---NRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNA--PDLWQYTPLHEAASKGRT 1009
+PN TD + D +AD S + ++++ L +A ++ + + + LL +VN + GRKSTPLH A+G+ R +V+ L+ GA+V KD+GGL+PLHNACS+GH +V ++L+ G+ NA D+W +TPLHEAA K + +VC +LL AD L N G +A D+A K T E+K LL+AAR + L +N + T LH A R ++ +LLL GA V+ K+K + PLH A H ++ + L + GA V P + + + E SK T
Sbjct: 251 DPNIRNTDGKSALD-LADPSAKAVLTGEYKKD--ELLEAARSGNEEKLMALL-TPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELKERLTYEFKGHSLLQAAREADMAKVKKTLALEIINFKHPQTHE-TALHCAVASPHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDILEVLQKHGAKVRVTRPHVTRLGSVQEKPSKVNT 550
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Cavefish
Match: ENSAMXT00000017800.2 (pep primary_assembly:Astyanax_mexicanus-2.0:16:21012912:21225385:-1 gene:ENSAMXG00000017269.2 transcript:ENSAMXT00000017800.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 229.18 bits (583), Expect = 6.111e-60 Identity = 235/854 (27.52%), Postives = 364/854 (42.62%), Query Frame = 2
Query: 212 TLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFT--------GEYKKQELLEAARNGLEETIMTILT-PFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVH-AKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILE----ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHS-------TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGA 2704
+ A + ++ V L G D+ + + G + LH A+ G ++V+ L+ RG++V + G LH A G A+VVK+L+ G+D NA+ +TPL+ A+ + DV LL+ + + DG T +A V + G+ + L AAR ++ +L N + + G TPLH+AA Y + LLL GA V + G+ PLH A G+ + + LL+RG+ ++A TPLH AA G LLL GA + SPL +AA + E C VK +LQ +D T +H + + + LL + A + L + G +PLH+A V +++LLV+ I G+T +H+AA + +I +L+ G++ + +G A + E + C L N ++E + IA + T ++ + +Q + A+ G L E D+ SVL+ + HS TPLH A+ + + V K+LL+ A + K GL PLH A Y + KVA LL+ A+ + +TPLH AA K + EI LLQY A+ + K G TPL L +EG D+ +KG VN+ + T LHLA+ + + +AE L++ GA ++ Q K G PL A YG+A M LLK +A+VN+ K GYTPLH+AAQ+ T + ++LL +GA
Sbjct: 35 SFLRAARAGNIDKVLEYLKGGVDIGTSNQNGLNA--LHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSG--FTPLHIAAHYGNVNVATLLLNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVE--------------C-------VKHLLQHKAPVDDVTLDYL----------------TALHVAAHCGHY-----RVTKLLLDKRANPNARALLQNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLT---PIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQVEVVR----------C--------------LLRNGAMVDARAREDQTPLHIASRLGKT---------EIVQLLLQHMAHPDAATTNGYTPLHISAREGQLDVASVLL---EAGASHSLATKKGFTPLHVASKYGSLDVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLASQEGHSDM---------AALLLQKG---------AQVNVPTKS----GLTALHLAAQEDRVGVAEILVKNGASMDQQTKLGYTPLIVACHYGNAKMVNFLLKSNASVNAKTKNGYTPLHQAAQQGNTHIINVLLQYGA 781 HSP 2 Score: 183.341 bits (464), Expect = 7.508e-46 Identity = 194/781 (24.84%), Postives = 316/781 (40.46%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPL-----------------------------HEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLAT------------DNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMV---------------------------------NAPDLWQ--YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTT---DIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKT-----DKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVL------VNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVK 2296
A K + +V+ LL+ G V+S G +T LH AS G+ E+V+ L+ +GA+++ + + G PL+ A H DVV+ L+ G + + +TPL H AA K +LLQ + ++++ G T +A + + FT L A ++ +L A TPLH AA V+LLLE GA + A+ K GL PLH A H+E K LL+ A V NA L Q +TPLH A K R +V LL+ YGA + +P+ +AA + H VL +L N + SN G+T +H + V ++ LLR GA ++ ++ +PLH+A +++QLL+++ + G LH++A + +L+ G++ ++ T +G+ + + +D+ + L ++ +A N + P + + N K DK S + P + K + + +Y + +K G L E +SD+ ++L VN G T LH AA +R+ V ++L++NGA + + K G PL AC YG+AK+ L++ NA+VN +TPLH+AA +G I +LLQY A P+ +G+T L + +
Sbjct: 72 AAKEGHMDLVQELLERGSSVDSATKKG--NTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALLQNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAA--------FMGHLNIVLL----------------LLQNGASPDVSNI-------------RGETALHMAARAGQV-----EVVRCLLRNGAM--VDARAREDQTPLHIASRLGKTEIVQLLLQH--MAHPDAATTNGYTPLHISAREGQLDVASVLLEAGASHSLATKKGFTPLHVASKYGSLDVAKLLLQRRAPPDSAG----KNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLASQEGHSDMAALLLQKGAQVNVPTKSGL--TALHLAAQEDRVGVAEILVKNGASMDQQTKLGYTPLIVACHYGNAKMVNFLLKSNASVNAKTKNGYTPLHQAAQQGNTHIINVLLQYGAKPNAITVNGNTALAIAR 798 HSP 3 Score: 150.984 bits (380), Expect = 4.789e-36 Identity = 105/307 (34.20%), Postives = 154/307 (50.16%), Query Frame = 2
Query: 1874 ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLC----SAADVRFLLE 2782
A++AG VE+V +L N + +V+ R + + TPLH A+ + +V++LL++ A A G PLH + G VA +L++ A+ ++ FTPLH A+ G ++ KLLLQ A P K+G TPL + D KV L+ + T TPLH+A+ N +EIA LL+ A+ N K G+ PLH AS GH+DMAALLL+ A VN K G T LH AAQ++R + +L+ +GA + G +PL + +A V FLL+
Sbjct: 467 AARAGQVEVVRCLLRNGA-----MVDARAREDQ--TPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLDVASVLLEAGASHSLATKKGFTPLHVASKYGSLDVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQ---------------------KVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLASQEGHSDMAALLLQKGAQVNVPTKSGLTALHLAAQEDRVGVAEILVKNGASMDQQTKLGYTPLIVACHYGNAKMVNFLLK 745 HSP 4 Score: 135.961 bits (341), Expect = 2.072e-31 Identity = 89/272 (32.72%), Postives = 148/272 (54.41%), Query Frame = 2
Query: 1979 TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKE-GDQDVYX------------XXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
TPLH A NR+ V+++L++ GA + A + GL P+H A GH + LL+Q A+ +V ++ T LH AA G+ E+ + LL+ A R ++ TPL + G ++ + L +A++GQ+ L+ + + T+ + TPLH+AS Y +L++A+ LL++ A ++ K GL PLH A+ Y + +A LLL A+ ++ K GYTPLH AA+KN+ ++ + LL + A+ + +G +PL L S
Sbjct: 396 TPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQVEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLDVASVLLEAGASHSLATK-KGFTPLHVASKYGSLDVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLAS 666 HSP 5 Score: 123.635 bits (309), Expect = 1.041e-27 Identity = 104/310 (33.55%), Postives = 153/310 (49.35%), Query Frame = 2
Query: 1874 ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRN--KDGHTPLD-------------LVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQG-RNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
ASK G+ +V +L+ S I + + DG TPLH AA + V++LLE GA + A+ K GL PLH A H + + L+Q+ A V+ L T LH AA G + + KLLL A+P+ R ++G TPL LVK G + + AA G + V L+ N D R T LH+A+ +E+ LL GA V+A+ + PLH AS G ++ LLL++ A+ ++A GYTPLH +A++ + + S+LL GA L +G +PL + S
Sbjct: 267 ASKRGNTNMVRLLLDRGSQI-----DAKTRDGL--TPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALLQNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIV--LLLLQNGASPDVSNIRGETALHMAARAGQVEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLDVASVLLEAGASHSLATKKGFTPLHVAS 567 HSP 6 Score: 120.168 bits (300), Expect = 1.133e-26 Identity = 98/319 (30.72%), Postives = 159/319 (49.84%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEG-DQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSV-----NINCRDTQGRNS-TPLHLASGYNNLEIAEFLLEQGADVN-AQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLLE 2782
L A++AG+++ V L+ DI + N + LH AA + +V+ LLE G+ V + K G LH A G A+V ++L++ A++N FTPL+ A+ + ++ + LL+ + +DG TPL + +++G +Q V LL +D+ IA + S N + D Q ++ TPLH+A+ Y N+ +A LL +GA V+ + G+ PLH AS G+ +M LLL + +++ + G TPLH AA+ LLL GA + G SPL + + D V+ LL+
Sbjct: 36 FLRAARAGNIDKVLEYLKGGVDIGTSNQNGLNA-------LHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQ 347 HSP 7 Score: 61.2326 bits (147), Expect = 1.063e-8 Identity = 41/127 (32.28%), Postives = 70/127 (55.12%), Query Frame = 2
Query: 2432 NSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLLEEAISQT 2800
N++ L A N ++ E+L + G D+ ++ GL LH A+ GH D+ LL+ ++V+SA K G T LH A+ + ++ +L+ GAD ++ G +PL + S + VR+LLE +Q+
Sbjct: 33 NTSFLRAARAGNIDKVLEYL-KGGVDIGTSNQNGLNALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQS 158
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009608.1 (pep scaffold:Pmarinus_7.0:GL477242:23155:61557:-1 gene:ENSPMAG00000008655.1 transcript:ENSPMAT00000009608.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 781.556 bits (2017), Expect = 0.000e+0 Identity = 440/864 (50.93%), Postives = 575/864 (66.55%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEINI-DMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAI 2791
+ LF+AC+N D+ VK L+ +VN++D GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHEAA+KGK DVCIVLLQ AD ++N+DG TA DLA + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + L++ GA VNA DLWQ+TPLHEAASK R EVCSLLL+YGADPT LNCH+KS ++LA T EL+E L +E +G L R + +VK+ L + +INF HP T +T +H ++S KRK + E LLR+GA ++N K +PLH+A + D+I +L+++ +IN+ D L G+ +LH AA + C +L+++G + +P+ QG+ A+QL + S ++ L ++ + +S+ + +L K ++ V+ +T N + +E + + A+ V +V +L++ +D V+ +D G PLH A + V ++L++ GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLDLVKEGD D+ DLL D+ALL+AAKKG +A+VQKL + N+NCRD+QGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KYSA VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A DVR LL +A+
Sbjct: 149 KELFEACRNGDVGRVKRLVSP-LNVNARDTTGRKSTPLHFAAGFGRKDVVEHLLQNGANVHARDDGGLIPLHNACSFGHAEVVNLLLRQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPGIRNTDGKTALDLA-EPTAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLVKHGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHSKSAIDLAPTSELKERLVYEFRGHSLLQAAREADVTRVKKHLGL----------------DIINFKHPQTHETALHCAVASP--YPKRKQVSELLLRKGA--NVNEKTKDLLTPLHVASDKAHNDVIDVLLKHGAKINVTDSL----GQTSLHRAAHAGHLTTCRLLLSHGCDPNLPSLQGFTAAQLGNE-------SVQQILHESSLPVRNSD-----VEHQLLEAAKAGDLDT------------VKQLCTTQTVNCRDMEGRHSTPLHFAAGYNRVSVVEYLLQHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVKRGAVVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSGENVNCRDSQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYSACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPSMKNQEGQTPLDLATADDVRALLIDAM 922 HSP 2 Score: 366.696 bits (940), Expect = 2.451e-106 Identity = 182/328 (55.49%), Postives = 239/328 (72.87%), Query Frame = 2
Query: 2993 LEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
L++++ FL+ + LD L ++F KEQ+T+D+L +MGH ELKE+G+ AYGHRHK++KG+ + + + G++P ++ H+ + TLL+DL P D +YQSVE++MQS+IR HKD GG+FNRY ++K++++ N+KLW++Y R++E+ EEN N NER+LFHGSPFI AI+N+GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC +HKDRSCYIC RQML CRV LGK+FLQ + +KMAH+PPGHHSV+GRPSV GL++AEYVIYRGEQAYP +LITY I P
Sbjct: 1003 LDMNINMFLKGLGLDHLRDIFEKEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGAQQGGLAYP---GANPYLSYHTTA--------------------------------QGTLLIDLPPDDKEYQSVEDEMQSTIREHKDGGQAGGVFNRYNVIKIQKVRNKKLWERYTHRRKEVTEENHNHHNERMLFHGSPFINAIINKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICQRQMLFCRVTLGKSFLQFSAMKMAHSPPGHHSVIGRPSVNGLAFAEYVIYRGEQAYPEYLITYQILKP 1295 HSP 3 Score: 258.84 bits (660), Expect = 1.799e-70 Identity = 183/521 (35.12%), Postives = 269/521 (51.63%), Query Frame = 2
Query: 194 FEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLH--FASGF-GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYK------KQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEE-INIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTF 1720
+E +L A + D+ VK L G D+ + + T LH AS + RK++ E L+ +GANV+ K + L PLH A H DV+ VL+ G+ N D T LH AA G C +LL D NL + G TA L ++ +++ + +LLEAA+ G +T+ + T VNC +GR STPLH AAGYNR +V+ LL+ GA VHAKDKGGLVPLHNACSYGH EVA+ L++RGA+VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N +PL+L ++++LL + L + + G +V Q L + + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ +N T LH AA+ + +C +L+ +G++ ++ +G L T D+ L + + P A ++ +PT
Sbjct: 451 YEFRGHSLLQAAREADVTRVKKHL--GLDIINFKHPQTHETALHCAVASPYPKRKQVSELLLRKGANVNEKTKDLLTPLHVASDKAHNDVIDVLLKHGAKINVTDSLGQTSLHRAAHAGHLTTCRLLLSHGCDPNLPSLQGFTAAQLGNESVQQILHESSLPVRNSDVEHQLLEAAKAGDLDTVKQLCTTQTVNCRDMEGRHSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKRGAVVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCSGENVNCRDSQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYSACVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPSMKNQEGQTPLDLATADDVRALLIDA--MPPEALPVTHKPPNIPTI 939
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 241.121 bits (614), Expect = 1.589e-66 Identity = 240/862 (27.84%), Postives = 366/862 (42.46%), Query Frame = 2
Query: 191 SFEENCRTLF-DACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVF--------TGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDE---LRELLYFEHKGF--VLFNY-------CRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKG--GLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGA 2704
SF+ + T F A +N +L+ + L G D+N+ + G + LH AS G ++V L+ R A + + G LH A G D+V+VL+ G++ NA+ +TPL+ AA + ++ LL A+ ++ DG T +A + V G+ + L AAR + +L N + TPLH+AA Y + LLL +GA V + G+ PLH A G+ + K LL+RGA ++ TPLH AA G +V +LL A + SPL +A E +LL V +Y G Y K +L G N + F T +H N R ++E LL+ GA S++ + G +P+H+A +++++QLL++N + V G+ LH+A+ + L+ G+ G +A + T + + L + E + SD N + + L+ AS+ G ++ +LEN +DI V + TPLH A+ + R+ V +LLE A K G GL PLH A Y H KVA LL+ A+ + +TPLH AA K + E+ K+LL+ A+ + + G PL L +EG D+ LLL A + A KG + T +HLA+ + + +AE L + ADVN K PLH AS YG+ M LL+ A VN+ + GYTPLH+AAQ+ T + + LL H A
Sbjct: 6 SFQSDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNA--LHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKD-DTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGF----------------TPLHIACKKN-----RIKVMELLLKHGA--SIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASS--NATNVRGETPLHMASRAGQLEVVKTLLQSGARV------GVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYF---------------------------------------------ASREGHGDVASLLLENGADISPV-------TKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGL----------------------TAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEA 759 HSP 2 Score: 190.274 bits (482), Expect = 6.121e-50 Identity = 206/801 (25.72%), Postives = 324/801 (40.45%), Query Frame = 2
Query: 467 ISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE----------------RGAMVNAPDLW-------------QYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNE----EINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTF---KSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQ--YNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
+ G D N + LH A+ +G DV LL+ +A+++ G TA +A G+ I+ +L F N +A TPL++AA N IV+ LL+ GA + G PL A GH +V LLE R NA L +TPLH AA G V +LLL GA + +PL +AA + G N VK +L G D+ + G T +H S + + +IE LL R A + K G SPLH+A +++ +QLL+ + ++ +D L LH+AA + +L++ G G+ + AC + K++ + + T ++ F G + V+ ++ +++ + AS+AG +E+V +L++ + + V R+ TPLH A+ + +V++LL+ G+D +A G PL+ A GH VA LL++ A+++ FTPLH A+ G+ E+ LLL+ +ADP ++G TPL + D KV L+ + T TPLH+A+ N LE+ + LLE GA+ N + G+ PLH A+ GHADM ALLL A+++ A K G T +H AAQ+++ + +L H AD +PL + S
Sbjct: 31 LKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIA-------ALAGQTD---------------IVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAA---------------------KRGNNNMVKLLLDR-GAKIDTKTRD---------------GLTPLHCAARSGH-----EKVIEMLLERDA--PILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYL------TPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHI--------------------ACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNV-VQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVG---VKAREDQ----TPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQ---------------------KVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVAS 710 HSP 3 Score: 132.88 bits (333), Expect = 1.304e-31 Identity = 129/468 (27.56%), Postives = 213/468 (45.51%), Query Frame = 2
Query: 1376 LNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQG----YRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRIL--ENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAA 2761
+N N+ G + LHLA +VD++ L++ E ID V G ALH+AA + I +LV +G+N + G Y A+Q +++ +FL LD + +N+ V T + T + + E + +ES K K V + I A++ D +L E+N+D+ T TPLH AA + ++V +LL GA V + G+ PLH A G+ + +LL+ A ++ TPLH AA G ++ ++LL+ +A + K+G +PL + +G+ +A VQ L+ D TPLH+A+ + +A+ LL++G + NA+ G PLH A + LLLK+ A++++ + G TP+H A+ + LLL +GA + N G++PL + S A
Sbjct: 37 INTCNQNGLNALHLASKEGHVDVVTELLK-REAEIDTATKV-GNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQ-ENHLEIVKFL-----LD------NGANQSVAT------EDGFTPLAVALQQGHEQVVGLLLESDTKGK------VRLPALHI--AARKDDTNAAALLLQHEHNADV---------TSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEH--------------------LACVQ-LLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRA 446 HSP 4 Score: 115.161 bits (287), Expect = 3.700e-26 Identity = 93/314 (29.62%), Postives = 147/314 (46.82%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYXXXXXXXXXXXXXKKGQI-------------AKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
L A++ G++E + L+ DI N + +G ++ LH A+ + VV LL+ A++ K G LH A G + +L+++ AN+N FTPL+ AA + EI K LL A+ +DG TPL + +++G + V LLL KG++ L+ N T TPLH+A+ Y N+ +A LL GA V+ + G+ PLH A+ G+ +M LLL A +++ + G TPLH AA+ ++ +LL A K G SPL + + +
Sbjct: 15 FLRAARNGNLERLMDHLKGGMDI-----NTCNQNGLNA--LHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDT------KGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGE 315 HSP 5 Score: 60.077 bits (144), Expect = 3.738e-9 Identity = 40/115 (34.78%), Postives = 63/115 (54.78%), Query Frame = 2
Query: 2468 NLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLLEEAISQT 2800
NLE L+ G D+N ++ GL LH AS GH D+ LLK A +++A K G T LH AA +T + +L+ GA+ ++ G +PL + + + V+FLL+ +Q+
Sbjct: 23 NLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQS 137
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 237.654 bits (605), Expect = 4.466e-65 Identity = 237/861 (27.53%), Postives = 362/861 (42.04%), Query Frame = 2
Query: 212 TLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVF--------TGEYKKQELLEAARNGLEETIMTIL---------TPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDE---LRELLYFEHKGF--VLFNY-------CRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGA 2704
+ A +N +L+ + L G D+N+ + G + LH AS G ++V L+ R A + + G LH A G D+V+VL+ G++ NA+ +TPL+ AA + ++ LL A+ ++ DG T +A + V G+ + L AAR +L + +N G TPLH+AA Y + LLL +GA V + G+ PLH A G+ + K LL+RGA ++ TPLH AA G +V +LL A + SPL +A E +LL V +Y G Y K +L G N + F T +H N R ++E LL+ GA S++ + G +P+H+A +++++QLL++N + V G+ LH+A+ + L+ G+ G +A + T + + L + E + SD N + + L+ AS+ G ++ +LEN +DI V + TPLH A+ + R+ V +LLE A K K GL PLH A Y H KVA LL+ A+ + +TPLH AA K + E+ K+LL+ A+ + + G PL L +EG D+ LLL A + A KG + T +HLA+ + + +AE L + ADVN K PLH AS YG+ M LL+ A VN+ + GYTPLH+AAQ+ T + + LL H A
Sbjct: 13 SFLRAARNGNLERLMDHLKGGMDINTCNQNGLNA--LHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESG--FTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGF----------------TPLHIACKKN-----RIKVMELLLKHGA--SIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASS--NATNVRGETPLHMASRAGQLEVVKTLLQSGARV------GVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYF---------------------------------------------ASREGHGDVASLLLENGADISPV-------TKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGL----------------------TAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEA 764 HSP 2 Score: 191.815 bits (486), Expect = 3.997e-50 Identity = 202/763 (26.47%), Postives = 322/763 (42.20%), Query Frame = 2
Query: 512 TPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-RNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLD-------------LVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
T AA G + + L+ D+N N +GL A LA+ V T K++ E+ A + G +T LH+AA + IV++L+E GA ++A+ + G PL+ A H+E+ K LL+ GA + +TPL A +G +V LLL K + L A L R + N +LQ N + + + ++N +G T +H N+ N+ LL GA +++ + G +PLH+A N ++++LL+ R +I+ +G+T LH AA + +L+ + T G + T + E+L AC+ G AP + T+ +Y + A+ G + +L+ + N R +G TPLH A NRI V+++LL++GA +H + GL P+H A GH V +LL+Q A+ N ++ TPLH A+ G+ E+ K LLQ A + ++ TPL L++ G + L A+++G V L+ + + TPLH+AS Y LE+A LLE+ A + + K GL PLH A+ Y H +A LLL A+ +S K GYTPLH AA+KN+ ++ +LL GA+ + +G +PL L +
Sbjct: 12 TSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVG-----------------------NTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLL---------ESDTKGKVRLPA----------------LHIAARKDDTNAAALLLQHEHNAD-------VTSKMMLNQT-TESGFTPLHIAAHYGNM-----NVATLLLNLGA--AVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLT---PLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMAT---------QGEHL----ACVQ---------------------------LLLGRQAPVDDVTL-----------DYLTPLHVAAHCGHYRVAKLLLDRGGE-----PNARALNG--FTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGH-GDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAA 649 HSP 3 Score: 190.274 bits (482), Expect = 1.062e-49 Identity = 203/807 (25.15%), Postives = 321/807 (39.78%), Query Frame = 2
Query: 467 ISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGA-------------------------------------MVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNE----EINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTF---KSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
+ G D N + LH A+ +G DV LL+ +A+++ G TA +A G+ I+ +L F N +A TPL++AA N IV+ LL+ GA + G PL A GH +V LLE M+N +TPLH AA G V +LLL GA + +PL +AA + G N VK +L G D+ + G T +H S + + +IE LL R A + K G SPLH+A +++ +QLL+ + ++ +D L LH+AA + +L++ G G+ + AC + K++ + + T ++ F G + V+ ++ +++ + AS+AG +E+V +L++ + + V R+ TPLH A+ + +V++LL+ G+D +A G PL+ A GH VA LL++ A+++ FTPLH A+ G+ E+ LLL+ A + K+G TPL + D KV L+ + T TPLH+A+ N LE+ + LLE GA+ N + G+ PLH A+ GHADM ALLL A+++ A K G T +H AAQ+++ + +L H AD +PL + S
Sbjct: 30 LKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIA-------ALAGQTD---------------IVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAA---------------------KRGNNNMVKLLLDR-GAKIDTKTRD---------------GLTPLHCAARSGH-----EKVIEMLLERDA--PILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYL------TPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHI--------------------ACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNV-VQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVG---VKAREDQ----TPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQ---------------------KVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVAS 715 HSP 4 Score: 148.288 bits (373), Expect = 2.016e-36 Identity = 134/465 (28.82%), Postives = 206/465 (44.30%), Query Frame = 2
Query: 245 QVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLE----------------------AARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATD---ELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQL-INFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYIL 1561
+V K LLD G + N++ G TPLH A R +++E L+ GA++ E GL P+H A GH +VV++L+ G+ NA +V TPLH A+ G+ +V LLQ+ A V +K A ++ T L K E+++ A+R G + ++L + + TPLH+A+ Y R + LLLE A K K GL PLH A Y H +VA LL++GA ++ YTPLH AA K + EV +LL GA+ L +PL LAA + ++ LL H L L + + + N + + K + +N T +H N+ ++ FLLR A +N + G +PLH A + ++ L+++E ++ G AL +A L S+ L
Sbjct: 358 RVAKLLLDRGGEPNARALNG--FTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVK----------AREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREG-HGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGL-------TAMHLAAQEDKVNVAEILTKHKADVNTTTKLC-YTPLHVASHYGNI-----KMVNFLLRNRAL--VNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELT--ANGNTALAIARRLGYISVVDTL 792 HSP 5 Score: 146.747 bits (369), Expect = 5.853e-36 Identity = 92/265 (34.72%), Postives = 136/265 (51.32%), Query Frame = 2
Query: 1979 TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTS---VNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD 2764
TPLH AA V K+LL+ G + +A+ G PLH AC KV ELL+++ A+++ TP+H A+ G + +LLLQ A + N G TPL + + GQ+ V+ L+ S V + R+ Q TPLH+AS EI + LL++G+D NA G PL+ AS GH D+A+LLL+ A+++ K G+TPLH A++ R ++ +LLL A A K G +PL + + D
Sbjct: 346 TPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMAS--------------------RAGQLEVVKTLLQSGARVGVKAREDQ----TPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYD 586 HSP 6 Score: 128.642 bits (322), Expect = 2.900e-30 Identity = 179/750 (23.87%), Postives = 292/750 (38.93%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYD--VNSKDNFGRKSTPLHFASGFGRKEMVEFLI---SRG----------------------------ANVSIK------DEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLAT-DNNTKLVFTGEYKKQELLEAARNGL-------------------------EETIMTILTPFNVNCHASDGRKS------------------TPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGY-------RASQLTTDIDLCEFLSEKEYL-----------------DPTAACISDSNKIVPTFKSKLFTNN---KTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILE-ASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELL 2131
L+ A + N L++VK LLD G + V ++D F TPL A G +++V L+ ++G A+V+ K E G PLH A +G+ +V +L++ G+ + TPLH AA +G ++ +LL A ++ K DGLT A + K++ + +L +NGL ++ + LTP +V H R + TPLH+A NR ++++LLL+ GA +H + GL P+H A GH+ V + LL+ GA NA ++ TPLH A+ G+ EV LL GA +++PL +A+ RLG+ V+ +LQ + N S G T ++ + ++ LL GA ++ + K+G +PLH+A +++ LL+ E+ +G G LH+A + + +L++ G++ GY + +QL L E +E L D A + I K L + + DK N V + + + + C L AS G++++V +L N + LVN + +G TPLH AA VV LL++ A + G L A G+ V + L
Sbjct: 113 LYMAAQENHLEIVKFLLDNGANQSVATEDGF----TPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIAS---------------------RLGKAEIVQLLLQRGSDPNAPTAS----------------GSTPLYFASREGH-----GDVASLLLENGA--DISPVTKKGFTPLHIASKYGRLEVATLLL--EKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVN-------------VAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRA-----LVNAKTRNGY--TPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 792 HSP 7 Score: 110.923 bits (276), Expect = 7.597e-25 Identity = 96/325 (29.54%), Postives = 153/325 (47.08%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYXXXXXXXXXXX--------XXKKGQIAKVQKLV-------TSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
L A++ G++E + L+ DI N + +G ++ LH A+ + VV LL+ A++ K G LH A G + +L+++ AN+N FTPL+ AA + EI K LL A+ +DG TPL + +++G + V LLL + AA+K L+ + + T TPLH+A+ Y N+ +A LL GA V+ + G+ PLH A+ G+ +M LLL A +++ + G TPLH AA+ ++ +LL A K G SPL + + + V+ LL
Sbjct: 14 FLRAARNGNLERLMDHLKGGMDI-----NTCNQNGLNA--LHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLL 331 HSP 8 Score: 60.077 bits (144), Expect = 3.708e-9 Identity = 40/115 (34.78%), Postives = 63/115 (54.78%), Query Frame = 2
Query: 2468 NLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLLEEAISQT 2800
NLE L+ G D+N ++ GL LH AS GH D+ LLK A +++A K G T LH AA +T + +L+ GA+ ++ G +PL + + + V+FLL+ +Q+
Sbjct: 22 NLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQS 136
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 192.971 bits (489), Expect = 4.553e-50 Identity = 230/877 (26.23%), Postives = 361/877 (41.16%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIP---TNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGE--IAPPVESTMKEKTTNDKQVEEYCMQILEASKA-GDVELVCRILENNSDIKS---VLVNCRDT-----------------DGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGG--LVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNIN--CRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLL----LAHGADAKLKNFEGQSPL 2743
L A N D V+ L+ +VN+ D+ K TPLH A+ G E++E L+ GA V+ KD L PLH AC+ V+ L+ +D NA+D TPLH AA ++ + N+ + G TA L AA NG E ++ L N +A D + +H AA +V++L+ GA V KDK G PLH A S G I V K LL+ G ++ P+ + T LH A G+ V + L+ GA+ N +PL AA H L ++ N D N V G + +H T + R + L++ G ++ ++ G++PLH+A + LI L+ N G+ G LHLAA S C L++ G +I +N+ ++ D EF + + + N ++ T NK D++ + V + + ++Q E C + A+ A GD V + N ++S V C + D + TP+H+AA + +++LLE + K G + PLH A +GH E+LI+ +++V + +TPL AA +G + ++L+Q+ A L+KEG +AL AA G + ++ LV + + N + TP+ LA G + + A LL +GA +NA+D G LH ++ GH + LLL+ S +V+ ++ G PLH AA + S L +A GA L + G +PL
Sbjct: 10 LIQAIFNGDPDEVRSLIYKKEEVNAVDD--EKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTA---------------------LHHAAFNGHSE-MVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAAS--------THGALCLE---------------LLVNNGADVN-------------VQSKDGKSPLHMT-----AVHGRFTRSQTLIQNGG--EIDCEDRDGNTPLHIAARYGHELLINTLITNGADTAK--RGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSFSNEHVLSAGFEIDTPD-EFGRTCLHAAASGGNVECLNLLLSTGADM----NKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGDGSTVSFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDADPGIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASV------------LLKEGPD-------RRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPL 793 HSP 2 Score: 182.956 bits (463), Expect = 5.349e-47 Identity = 236/910 (25.93%), Postives = 365/910 (40.11%), Query Frame = 2
Query: 116 NEYCTDIQDICDKIADTSLSKLTEASFEENCR------TLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRI-VQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA----TDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVD-LIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLC-EFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEP---KFEGEIAPPVESTMKEKTTNDKQVEEYCMQIL----EASKAGDVELVCRILENNSDIKSVLVN------CRDTDGRHSTPLHFAAGFNRISVVKVLLENGAD---VHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKE-GDQDVYXXXXXXXXXXXXXKKGQIAKVQ-KLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKG-GLIPLHNASSYGHADMAALLL-KYSAN-VNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPL 2743
N++ T + C ++ ++ L + S + N R L A N ++ + ++ + N D GR T LH A+ G EMV FL+++GAN++ D+ +H A GH +VVKVL++ G+D KD Y+PLH A+ G+ V +LL D++ NS G TA +A N +V EL++ N + ++ R TPLH AA + ++LL+ GA V+ + K G PLH +G +++L++ G ++ D TPLH AA G + + L+ GAD H PL LAA +D R+LL K ++ ++ +N+ S + F G T +HA S NV + LL GA +N ++ + LH A NV ++ L+ +N T LH AA + V++ TI ++ +C E L K+ DP I D P + + + + ++P + +G+ P+ E ++ L G L L ++D VL+ ++ R T LH AA + +++L+EN V A D P+ A GH A LL+ A++N D T LH AA G E LLLQ + D R + G PL + G + A VQ + + I D G TPLH A + E LLE DV + +G PLH A + A +L+ + A +N D G PLH AA + + LLL+H A + + G+SPL
Sbjct: 70 NKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGR--TALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVV------NELIDCG----------------ANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCEDRDGNTPLHIAARYGHELLINTLITNGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSF------------------SNEHVLSAGFEIDTPDEF-----GRTCLHAAASGGNV-----ECLNLLLSTGA--DMNKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCT---PLHYAAAADGDG---STVSFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDA-DPG---IQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKEGPDRR-TALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILS-----------------ALVQAAMASGAPIPLTDRNGY--TPLHWACYNGHDGCVELLLE--LDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPL 893 HSP 3 Score: 112.464 bits (280), Expect = 3.188e-25 Identity = 92/299 (30.77%), Postives = 147/299 (49.16%), Query Frame = 2
Query: 1946 VNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLD-------------LVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVT-SVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLC-SLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQ 2797
VN D + R TPLH AA ++++L+ +GA V++KD L PLH AC+ + L++++A+VN D TPLH AAA + + ++ + + ++ G T L LV +G A+ AA G V+ LV ++ C+D +G +PLH AS + + + LL+ G D++ + G LH A G + L+ ANVN A++ G+TPLH AA LC LL+ +GAD +++ +G+SPL + +A RF + + Q
Sbjct: 32 VNAVDDEKR--TPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGY--SPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQSKDGKSPLHM-TAVHGRFTRSQTLIQ 325 HSP 4 Score: 112.464 bits (280), Expect = 3.653e-25 Identity = 119/431 (27.61%), Postives = 188/431 (43.62%), Query Frame = 2
Query: 314 TPLHFASGFGRKEMVEFLISR-GANVSIKDEGGLI-PLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVN--CHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNY-CRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-RNEEINI-DMLEGVTGKNALHLAAELNSSSICYILVNYGSNKT 1585
TP+H+A+ +G + +E L+ + GA S G I PLH A GH ++VLI + D + ++ YTPL AA++G D VL+Q A V LK G ++ L AA NG E + ++ + N ASD + TP+ LA G LLL GA ++AKD G LH + GH E LL+R V+ + PLH AA+ G + S L+ + +P+ L + G+ ++ C G V+ +L++ + KQ+ F + +H + ++N ++ E L+ +N + +G PLH A T +V+ +QLL+ N +N+ DM GK+ L +A E ++ +LVN S KT
Sbjct: 550 TPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKE--------------------GPDRRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMA-------SGAPIPLT-----------DRNGYTPLHWACYNGHDGCVELLLEL----------DVFQKQEGNAF-------SPLHCAVLNDN-----ESCAEMLIDTFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDM----AGKSPLMMACENGQATAVEVLVN--STKT 914 HSP 5 Score: 111.309 bits (277), Expect = 6.915e-25 Identity = 83/270 (30.74%), Postives = 123/270 (45.56%), Query Frame = 2
Query: 1949 NCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIA-EFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
N D GR T LH AA +V L+ GA+++A DK +H A GH +V ++L+ + A+V D ++PLH A++ G+ + KLLL D N G+T L + QDV V +L+ N R TPLH A+ + + E L+ GADVN Q K G PLH + +G + L++ ++ D+ G TPLH AA+ L + L+ +GAD + G PL L +
Sbjct: 132 NVSDRAGR--TALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVV--------------------VNELI-DCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCEDRDGNTPLHIAARYGHELLINTLITNGADTAKRGIHGMFPLHLAA 378 HSP 6 Score: 105.531 bits (262), Expect = 4.465e-23 Identity = 167/733 (22.78%), Postives = 269/733 (36.70%), Query Frame = 2
Query: 818 VQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSN--------FIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVL----------NKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVT-GKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTD-KWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVA------------------ELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCR--DTQGRNSTPLHLASG------------YNNLEIA----------------EFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGY--TPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLLEEAIS 2794
V+ L+ +V+A D PLH A G E+ + L+ GA VN+ D TPLH A + LL + AD + + ++PL +AA + + + ++ ML ++N S+ + F +++NF L+ + N K + I + G T + VL +K+G SPLH A + + +++LL+ ++ +D+ + + G ALH+A + L++ G+N +G+ F + + A C+ +L NN D SK+ K P+ T Q +++N +I +C D DG +TPLH AA + ++ L+ NGAD + G+ PLH A G + E ++ ++ D + T LH AA+ G E LLL AD +K+++ T L VQ +VT V R + R TPLH A+ YN + + E LL + AD QDK G P+H A++YGH LLL+ S K G+ +PLH AA +L+ D ++ G +PL L + VR L++ S
Sbjct: 22 VRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKC---------------------AEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNF--------LVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLL---DLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTP---------LHFAAASTH---GALCL------------ELLVNNGADVNVQSKDGK------SPLHMTAVHGRFTRSQT---------------------LIQNGGEI-----DCEDRDG--NTPLHIAARYGHELLINTLITNGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSFSNEHVLSAGFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKDRFERTALHYAAAN-----------------------CNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGDGSTVSFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDADPGIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGAS 641 HSP 7 Score: 100.523 bits (249), Expect = 1.444e-21 Identity = 162/682 (23.75%), Postives = 270/682 (39.59%), Query Frame = 2
Query: 878 PLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGA----------DPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKR----MLQMLGNTNDSNCSN--------FIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVL----------NKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVT-GKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRA---SQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGR--HST-PLHFAA--GFN-----------RISVVKV-----LLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQD-------VYXXXXXXXX--XXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGG--LIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLK 2719
PL A G + +SL+ + VNA D + TPLH AA G E+ LL+ GA P H C ++S E A L+ + + N+ + ++ ML ++N S+ + F +++NF L+ + N K + I + G T + VL +K+G SPLH A + + +++LL+ ++ +D+ + + G ALH+A + L++ G+N +G+ + +T LC L D + + T FT ++T N E ++ ++ T Y ++L I +++ N DT R H PLH AA GF+ + S+V +L G ++ D+ G LH A S G+ + LL+ A++N D ++ T LH AAA + L+ A +++++ TPL D D VY+ + S + + QI L + +D QG TP+H A+ Y + E LLEQ + K G + PLH A+ +GH +L++ +++ + GYTPL AA + T +L+ HGA LK
Sbjct: 9 PLIQAIFNGDPDEVRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRS--EAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNF--------LVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLL---DLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGE----------IDCEDRDGNTPLHIAARYGHELL--------------------INTLITNGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSFSNEHVLSAGFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGDGSTVSFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDADPGIQDKQGY--TPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLK 645 HSP 8 Score: 93.2041 bits (230), Expect = 2.993e-19 Identity = 74/242 (30.58%), Postives = 113/242 (46.69%), Query Frame = 2
Query: 2081 PLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVT-SVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLLEEAI 2791
PL A G LI VN D K TPLH AA G+ EI +LL+ A + ++ TPL + A VQ L+ S ++N RD + TPLH+A+ ++ AE ++ + N D+ G LH+A+ GH++M L+ AN+N+ DK +H AA T++ +L+AHGAD + K+ +G SPL S++ V+ LL+ +
Sbjct: 9 PLIQAIFNGDPDEVRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSE--------------------AAVQTLLKHSADVNARDKNWQ--TPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGV 228 HSP 9 Score: 92.4337 bits (228), Expect = 4.499e-19 Identity = 106/377 (28.12%), Postives = 172/377 (45.62%), Query Frame = 2
Query: 416 PLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNK---SPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIH-ATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDL-IQLLVRN-EEINIDMLEGVTGKNALHLAA 1528
PL A G D V+ LI + NA D TPLH AA G+A++ +L+ + A VN K++ LT A A+R+ E + T+L + + +A D TPLH+AA + + ++ M + + D+ G LH+A GH E+ L+ +GA +NA D +H AA G TEV +L+A+GAD + C +K SPL A++ G+ VK +L + + +D N +F G+T +H A + +V+ + L+ GA ++N N++G +PLH A + + L ++LLV N ++N+ + GK+ LH+ A
Sbjct: 9 PLIQAIFNGDPDEVRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRAC-------------------ASRS--EAAVQTLLK-HSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGAD---VRCKDKKGYSPLHAASSS---------------------GQIGVVKLLLDLGVDIDDPN-----------SF-----GNTALHVACYNGQDVV------VNELIDCGA--NVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNV---QSKDGKSPLHMTA 312 HSP 10 Score: 79.7221 bits (195), Expect = 3.356e-15 Identity = 82/314 (26.11%), Postives = 115/314 (36.62%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNA----KDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLER-------------------------------------GAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGA 1042
A N LL G +N+KDN G T LH + G +E V+ L+ R +VS+++ G PLH A + GH ++ L+ A A D YTPLH A G D C+ LL + + + A N+ + E ++ +NC + GR PLH AA + +QLLL A V+ D G PL AC G + L+ ++NA + TPLH AA G V +LL GA
Sbjct: 693 AVGNGHTDCASLLLSKGASINAKDNKG--CTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGH-DGCVELLLELDVFQKQEGNAFSPLHCAVLNDNE------------------SCAEMLIDTFGAKIINCPDAKGR--MPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLVNSTKTDLKLQDANRNTALHLACSRSHETCALLILDKLDDLNLINATNAALQTPLHLAARSGMATVVQVLLNKGA 983 HSP 11 Score: 79.337 bits (194), Expect = 4.256e-15 Identity = 81/292 (27.74%), Postives = 124/292 (42.47%), Query Frame = 2
Query: 212 TLFDACKNNDLQVVKCLL---DIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAK----ADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQE------LLEAARNGLEETIMTILTPFN---VNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAM-VNAPDLWQYTPLHEAASKGRTEVCS 1021
L A N ++ ++ L+ D V++ D + TP+ A G G + L+S+GA+++ KD G LH + GH + V +L+ D + ++ PLH AA G + L+QA A + L + +G T A N L+ ++KQE L A N E ++ F +NC + GR PLH AA + +QLLL A V+ D G PL AC G + L+ + D + T LH A S+ E C+
Sbjct: 653 ALHSAATNGHVECLRLLVENADSNEAVDASD--ANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGR--MPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLVNSTKTDLKLQDANRNTALHLACSRSH-ETCA 939 HSP 12 Score: 59.3066 bits (142), Expect = 5.739e-9 Identity = 67/280 (23.93%), Postives = 101/280 (36.07%), Query Frame = 2
Query: 200 ENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGR----KEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLIS----------------------------------AGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNN----TKLVFTGEYKKQELLEAARNGL--------EETI---MTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVP 880
+ C L + V LL DV+ ++ GR PLH A+ G +V+ ++ GA + + D G PLH AC GH V++L+ N D PLH AA + +LL A VN+ + G + +A +N +++ +L +A RN ET + +A++ TPLHLAA +VQ+LL GA + A D G P
Sbjct: 718 KGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGR--APLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLVNSTKTDLKLQDANRNTALHLACSRSHETCALLILDKLDDLNLINATNAALQTPLHLAARSGMATVVQVLLNKGASILAVDHNGYTP 995
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 179.489 bits (454), Expect = 9.349e-46 Identity = 143/496 (28.83%), Postives = 225/496 (45.36%), Query Frame = 2
Query: 1322 SKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN----EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRH-STPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDL-CSAADVR---FLLE 2782
S + ++E LL +GA + K G SPLH+A +V+ +Q+L++ ++I +D L ALH+AA +L++ G+N + G+ + + + + +++S + P S N N +G +P + E + A++AG E+V +L N + + D R TPLH AA ++ +V++LL++ A A G PLH A GH V +L+++ A++ V FTPLH A+ GK E+ LLLQ A P K+G TPL + D KV L+ + T TPLH+A+ + LEIA LLE GA+ NA + G+ PLH + GHAD+A+LLL A+VN A K G +PLH AAQ+++ + +L+ HGA G +PL + C +++ FLL+
Sbjct: 16 SGHEQVVEILLEQGA--PILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYL------TALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESG-LTPMHVSAFMGN-----LNIVLILLQGGASPNATNVRGETALH------------MAARAGQTEVVRSLLRNGAQV--------DAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQ---------------------KVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQ 456 HSP 2 Score: 140.584 bits (353), Expect = 1.001e-33 Identity = 120/414 (28.99%), Postives = 176/414 (42.51%), Query Frame = 2
Query: 185 EASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDE---LRELLYFEHKGFV----------LFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVL 1387
EA E + + +L +V LL G N+ + R T LH A+ G+ E+V L+ GA V K PLH A G ++V++L+ + P+A YTPLH AA +G DV +LL+ A + + G T +A+ +Y K E + ++L + A+ TPLH+A+ Y+ ++ LLL+ GA HA K G PLH A +E+A +LLE GA NA PLH A +G +V SLLLA AD H SPL LAA ++ + E+L +H V L C G V +LQ + N + + Q H + L+ N+V + R G S ++ L
Sbjct: 132 EAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNV--RGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVAS----------KYGKME------------VASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEIL-VKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISVVDTL 520 HSP 3 Score: 140.198 bits (352), Expect = 1.228e-33 Identity = 139/477 (29.14%), Postives = 209/477 (43.82%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDL-ATDNNTKLVF-----------TGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEF---LLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-RNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGY 1606
A ++ QVV+ LL+ G + +K G +PLH A+ E V+ L+ A V L LH A GH KVL+ G++PN + + +TPLH A K + V +LL+ A + GLT + A N +V T + L AAR G E + ++L A + TPLH+AA + IVQLLL+ A A G PLH A GH +V LLE GA + +TPLH A+ G+ EV SLLL A P + +PL +A+ +++++ KV +L G + + N G T +H I+ +K+ +E LL GA + N + +QG +PLHL + D+ LL+ R ++N G++ LHLAA+ + ++ ILV +G++ T GY
Sbjct: 13 AARSGHEQVVEILLEQGAPILAKTKNGL--SPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRN-GAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVAS--------HYDNQ--------------KVALLLLDQGASPHATAKN---------------GYTPLH--------IAAKKDQLEIATTLLEYGAET--NAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLS---PLHLAAQEDKVNVAEILVKHGAHVNATTKLGY 436 HSP 4 Score: 131.339 bits (329), Expect = 6.080e-31 Identity = 97/271 (35.79%), Postives = 142/271 (52.40%), Query Frame = 2
Query: 1979 TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAK--VQKLVTSVNINCRDTQG---------RNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
TPLH AA VV++LLE GA + AK K GL PLH A H + ++L+QY A V+ L T LH AA G + K+LL A+P+ R +G TPL + K+ V +LLL A +EA + + V + ++NI QG R T LH+A+ E+ LL GA V+A+ + PLH A+ G ++ LLL++ A+ ++A GYTPLH AA++ + ++LL HGA + +G +PL + S
Sbjct: 8 TPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVAS 278 HSP 5 Score: 125.176 bits (313), Expect = 5.121e-29 Identity = 135/541 (24.95%), Postives = 227/541 (41.96%), Query Frame = 2
Query: 854 AKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFI--------DKQQLINFVHPF---------TGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN---------EE-------INIDMLEGVT---------------GKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLS--EKEYLDPTAACISDSNKIVPTFKSKLFTNNKT-----DKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHST-----PLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVK 2296
A + GL PLH A GH +V + LLE+GA + A +PLH A E +L+ Y ++P++ D L L H G Y K +L N N + F ++ +++ + + +G T +H + N+ N++ LL+ GA S N N +G + LH+A ++++ L+RN EE + LE V G LH+AA + IL+ +G++ + T +G+ + + E S + + P AA N + P + + N K D+ S + P + K++ + EY + ++ G L E ++D+ S+L+ R D H+T PLH AA ++++V ++L+++GA V+A K G PL AC YG+ K+ L+Q+ A+VN +TPLH+AA +G I LLL+ A P+ +G+T L + +
Sbjct: 1 AHRQDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQY-----------RAPVDDITLDYLTALHVAAH----------CGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNL-----NIVLILLQGGA--SPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAA---GKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLL-ARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIAR 509
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464]) HSP 1 Score: 74.3294 bits (181), Expect = 4.076e-15 Identity = 54/148 (36.49%), Postives = 79/148 (53.38%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLD--IGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAG-SDPNAKDVWNYTPLHEAAVKGKADVCIVLLQA-KADVNLKNSDGLTAFDLATDNNTKLVF 655
AC +L+VVK L D + D+N N G T LH A G E+ +FLI GA+V IKD+ IPLH A S G ++++L G S N +D +TPL A +G D ++L++ A+ +L ++ G A D+A + K F
Sbjct: 79 ACSVGNLEVVKSLYDRPLKPDLNKITNQG--VTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKGAKAEDVALNEQVKKFF 224 HSP 2 Score: 72.4034 bits (176), Expect = 1.994e-14 Identity = 78/272 (28.68%), Postives = 110/272 (40.44%), Query Frame = 2
Query: 1982 PLHFAAGFNRISVVKVLLENGADVH-AKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDL-----WKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALL--LKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLL-AHGADAKLKNFEGQSPLDLCSAADVR 2770
PLH A N V+ LL + + KD+ G +PLH + S+ ++ L+ NVN+ D W TP H A + G E+ K L P DL K +T+ + C LHLA G E+++FL+E GA V +DK IPLH A+S G + LL L SA VN DK G+TPL A + LL+ +GA+ L + +G D+ V+
Sbjct: 5 PLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGW--TPFHIACSVGNLEVVKSLYDRPLKP-----------DLNK-------------------------------ITNQGVTC----------LHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSA-VNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKGAKAEDVALNEQVK 221 HSP 3 Score: 61.2326 bits (147), Expect = 1.355e-10 Identity = 62/218 (28.44%), Postives = 88/218 (40.37%), Query Frame = 2
Query: 416 PLHNACSFGHADVVKVLISAGSDPN---AKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNL---KNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERG-AMVNAPDLWQYTPLHEAASKGRTEVCSLLL-AYGAD 1045
PLH AC + KV S P+ KD PLH + ++ LL +VNL + G T F +A V Y + P + + + T LHLA G + Q L+E GA V KDK +PLH A S G +++ + L G + VN D +TPL A ++G + LL+ YGA+
Sbjct: 5 PLHQACM--ENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDR--------------------PLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAE 200 HSP 4 Score: 55.4546 bits (132), Expect = 9.128e-9 Identity = 38/92 (41.30%), Postives = 49/92 (53.26%), Query Frame = 2
Query: 1979 TPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNAN-VNVCDLWKFTPLHEAAAKGKFEICKLLLQ-YNAD 2248
T LH A G V + L+ENGA V KDK +PLH A S G K+ ELL + VN D +TPL A A+G + LL++ Y A+
Sbjct: 109 TCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAE 200 HSP 5 Score: 55.4546 bits (132), Expect = 9.299e-9 Identity = 45/134 (33.58%), Postives = 65/134 (48.51%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVN-SKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSI---KDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWN--YTPLHEAAVKGKADVCIVLLQAKADVNLKN 598
L AC N+ V+ LL + KD GR PLH++ F E+ FL+S+ NV++ D+ G P H ACS G+ +VVK L P+ + N T LH A K +V L++ A V +K+
Sbjct: 6 LHQACMENEFFKVQELLHSKPSLLLQKDQDGR--IPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKD 137
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374]) HSP 1 Score: 73.1738 bits (178), Expect = 2.256e-13 Identity = 45/116 (38.79%), Postives = 64/116 (55.17%), Query Frame = 2
Query: 1955 RDTDGRHSTPLHFAAGFNRISVVKVLLENGA-DVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKF--TPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV 2293
RD+ GR T L A + VVK ++E G D++ +D G LH A GH ++ ELLI+ A+VN+ + F TPL +A+A G ++ K LL+ ADP RN G T + V
Sbjct: 326 RDSGGR--TRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPTIRNAKGLTAFESV 439 HSP 2 Score: 65.4698 bits (158), Expect = 4.729e-11 Identity = 41/114 (35.96%), Postives = 66/114 (57.89%), Query Frame = 2
Query: 290 KDNFGRKSTPLHFASGFGRKEMVEFLISRGA-NVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNY--TPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFD 622
+D+ GR T L A G+ ++V+ +I G +++ +D G LH A GH ++V++LI G+D N K + + TPL +A+ G DV LL+ AD ++N+ GLTAF+
Sbjct: 326 RDSGGR--TRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPTIRNAKGLTAFE 437 HSP 3 Score: 60.4622 bits (145), Expect = 1.818e-9 Identity = 38/105 (36.19%), Postives = 56/105 (53.33%), Query Frame = 2
Query: 758 SDGRKSTPLHLAAGYNRCRIVQLLLEMGA-QVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQY--TPLHEAASKGRTEVCSLLLAYGADPTHLNC 1063
S GR T L +A + +V+ ++E G ++ +D G LH A GHIE+ + L+E GA VN + + TPL +A++ G +V LL GADPT N
Sbjct: 328 SGGR--TRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPTIRNA 430 HSP 4 Score: 60.4622 bits (145), Expect = 1.996e-9 Identity = 37/109 (33.94%), Postives = 61/109 (55.96%), Query Frame = 2
Query: 2414 RDTQGRNSTPLHLASGYNNLEIAEFLLEQGA-DVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVN--SADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEG 2731
RD+ GR T L +A ++ + ++E+G D+N QD G LH A+ GH ++ LL++ A+VN S + +G TPL +A+ + LL +GAD ++N +G
Sbjct: 326 RDSGGR--TRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPTIRNAKG 432 HSP 5 Score: 50.447 bits (119), Expect = 2.279e-6 Identity = 31/85 (36.47%), Postives = 47/85 (55.29%), Query Frame = 2
Query: 2360 KGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQ--DKGGLIPLHNASSYGHADMAALLLKYSAN 2608
KG+ V+K++ + D +T LH A+ ++EI E L+E GADVN + + G PL +AS+ GH D+ LLK A+
Sbjct: 340 KGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGAD 424
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672]) HSP 1 Score: 65.855 bits (159), Expect = 3.052e-11 Identity = 61/225 (27.11%), Postives = 92/225 (40.89%), Query Frame = 2
Query: 200 ENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLH----------------NACSFGHADV----------------------VKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNT------KLVFTG-EYKKQELLEAARNGLEETIMTILTPF 739
E+ L A NN L VV L+ G DVN++ +TPLH+A+ +G +V+FL+ GA+ ++ D+ G LH N S G D+ V L+ G+ D +TPLH VKG+ V L+Q AD K G F +A + NT L +G +Y + + + ++T +TPF
Sbjct: 108 EHITGLHWASINNRLSVVDFLVSQGADVNARAG-ALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIAQEMNTVYSLREALTHSGFDYHGYPIKKWFKKSQHAKLVTFITPF 331 HSP 2 Score: 64.6994 bits (156), Expect = 8.923e-11 Identity = 69/272 (25.37%), Postives = 112/272 (41.18%), Query Frame = 2
Query: 1805 ESTMKEKTTNDKQVEE--YCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGL--VPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSAN 2608
E + E+ ++ + EE + A + GD+ V ++ K + VN H T LH+A+ NR+SVV L+ GADV+A+ G L PLH A YG+ + + L+++ A+ + D F LH + + +L ++V +G D I+CRD +GR S L A ++L +AE LL+ GA + D G PLH + G + L++ A+
Sbjct: 55 EESGNEQVNHNDEAEEDPLLTRYHTACQRGDLATVKEMIHG----KLLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNAR-AGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVL----------------FNVVSKGLLD-------------------------------IDCRDPKGRTSL-LWAAYQGDSLTVAE-LLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGAD 272 HSP 3 Score: 61.2326 bits (147), Expect = 1.035e-9 Identity = 40/132 (30.30%), Postives = 67/132 (50.76%), Query Frame = 2
Query: 2357 KKGQIAKVQKLVTS--VNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGL--IPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSP 2740
++G +A V++++ + +N + T LH AS N L + +FL+ QGADVNA+ G L PLH A+ YG+ + LLK+ A+ D G+ LH + + L +L + L + + + P
Sbjct: 82 QRGDLATVKEMIHGKLLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNAR-AGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDP 212 HSP 4 Score: 55.8398 bits (133), Expect = 3.780e-8 Identity = 48/184 (26.09%), Postives = 75/184 (40.76%), Query Frame = 2
Query: 2366 QIAKVQKLVT-SVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLH----------------NASSYGHADM----------------------AALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQT 2800
+++ V LV+ ++N R ++TPLH A+ Y + I +FLL+ GAD D G LH N S G D+ A LLK+ A++ AD G+TPLH K + + L+ GAD K G+ + + + L EA++ +
Sbjct: 121 RLSVVDFLVSQGADVNAR-AGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIAQEMNTVYSLREALTHS 303 HSP 5 Score: 54.299 bits (129), Expect = 1.232e-7 Identity = 51/198 (25.76%), Postives = 78/198 (39.39%), Query Frame = 2
Query: 1823 KTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLH----------------NACSYG-----------------------HAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKE 2299
+ ND E+ + AS + +V ++ +D VN R H+TPLH+AA + + +V LL++GAD D G LH N S G VAELL ++ A++ + D FTPLH KG+ + K L+Q AD ++ G + +E
Sbjct: 99 EVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGAD-----VNAR-AGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELL-KFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIAQE 289
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Yeast
Match: GDE1 (Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes [Source:SGD;Acc:S000006031]) HSP 1 Score: 60.077 bits (144), Expect = 2.360e-9 Identity = 60/236 (25.42%), Postives = 102/236 (43.22%), Query Frame = 2
Query: 2081 PLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDK-GGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSL------LLAHGADAKLKNFEGQSPLDLCSAADV 2767
PLH +C YG ++V +L+I+ N+ W P+ + +A G E L H + + L++ D +V S L + V +IN +D + + TPL+LA N E A LL GAD+ ++K G + A++ G D+ LL+ +AN + D+ G+TP+ A + + + L+ H E + PL+ SA ++
Sbjct: 431 PLHYSCQYGLSEVTKLIIKLMKEWNI---WNEIPIDDVSAFGDAESLTPLHLCVLGAHPKTTEV-----LLQSLDPNVKLKSSSLLHLATEWNNYPLLHVLLSSKRFDINYQDNE-LHETPLYLACRLNFFEAAVCLLYNGADLEIREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADMVQIRDELVTHPHSQLNSGSEEKEPLNEISAGEL 657 HSP 2 Score: 56.9954 bits (136), Expect = 2.021e-8 Identity = 62/237 (26.16%), Postives = 100/237 (42.19%), Query Frame = 2
Query: 293 DNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQA-KADVNLKNSDGLTAFDLATD-NNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVN----CHASDGRK---------STPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAP 958
DN+ R TPLH++ +G E+ + +I +I +E IP+ + +FG A+ + TPLH + VLLQ+ +V LK+S + LAT+ NN L+ K+ + N L ET + + N C +G T + +AA IV+LL+ A +D+GG P+ +A GH+ +A + R +V P
Sbjct: 425 DNYKR--TPLHYSCQYGLSEVTKLIIKLMKEWNIWNE---IPIDDVSAFGDAE------------------SLTPLHLCVLGAHPKTTEVLLQSLDPNVKLKSS---SLLHLATEWNNYPLLHVLLSSKRFDINYQDNELHETPLYLACRLNFFEAAVCLLYNGADLEIREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADMVQIRDELVTHP 635 HSP 3 Score: 51.2174 bits (121), Expect = 1.120e-6 Identity = 30/96 (31.25%), Postives = 48/96 (50.00%), Query Frame = 2
Query: 1946 VNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDK-GGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLL 2230
+N +D + H TPL+ A N LL NGAD+ ++K G + A + G + +LLI NAN ++ D +TP+ A +G I ++
Sbjct: 531 INYQDNE-LHETPLYLACRLNFFEAAVCLLYNGADLEIREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADMV 625 HSP 4 Score: 50.447 bits (119), Expect = 2.058e-6 Identity = 32/98 (32.65%), Postives = 54/98 (55.10%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDN-FGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGH---ADVVKVLISAGSDPNAK 496
L+ AC+ N + CLL G D+ ++ FG T + A+ G ++V+ LI+ AN I+DEGG P+ +A GH AD+V++ + P+++
Sbjct: 543 LYLACRLNFFEAAVCLLYNGADLEIREKLFGW--TAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADMVQIRDELVTHPHSQ 638
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Yeast
Match: YCR051W_mRNA (Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene [Source:SGD;Acc:S000000647]) HSP 1 Score: 51.2174 bits (121), Expect = 2.586e-7 Identity = 32/105 (30.48%), Postives = 53/105 (50.48%), Query Frame = 2
Query: 2444 LHLASGYNNLEIAEFLLEQGADV---NAQDKGGLIPLHNASSYGHADMAALLL-KYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLD 2746
+ +A+ NL+ E +L + ++D G P+H A++YGH D+ + +Y+ ++N D G TPLH +L L G D ++N EGQ+P D
Sbjct: 5 IWVAASDGNLDRVEHILRESKGAMTPQSKDINGYTPMHAAAAYGHLDLLKKMCNEYNGDINVLDNDGDTPLHHVEDVATARLIVEEL--GGDFTIRNVEGQTPYD 107
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Nematostella
Match: EDO43690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXV8]) HSP 1 Score: 109.768 bits (273), Expect = 3.673e-26 Identity = 70/229 (30.57%), Postives = 119/229 (51.97%), Query Frame = 2
Query: 305 RKSTPLHFASGF--------GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIM--TILTPFNVNC--HASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNA 955
RK + F SG G + V+ L+++GA+++ ++ GL LH AC + D+VKVL+ G+ + +D +T LH AA G ++ L++ ADV N++G DLA + + + + T E + Q + +EE IM + N NC + D +++ LH+A+ +++ LLL++G +++AKD PLH A +G + + L E GA NA
Sbjct: 40 RKKPKIQFNSGIVFLSAVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEEKIMLNDAQSWLNKNCINESKDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDACELLSENGADFNA 268 HSP 2 Score: 92.0485 bits (227), Expect = 3.992e-20 Identity = 64/210 (30.48%), Postives = 107/210 (50.95%), Query Frame = 2
Query: 2012 ISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGD-QDVYXXXXXXXXXXXXXKKGQIAKVQ--KLVTSVNINC--RDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADK 2626
+ VK LL GAD++ ++ GL LH AC + + ++L+ A+++V D +T LH AA+ G EI K L++ AD N +G PLDL +E D +D+ + + + + K+ + +N NC ++ LH+AS L++ LL+ G ++NA+D PLH A+ +G D LL + A+ N+ +K
Sbjct: 62 VDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEEKIMLNDAQSWLNKNCINESKDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDACELLSENGADFNARNK 271 HSP 3 Score: 67.0106 bits (162), Expect = 9.954e-12 Identity = 61/218 (27.98%), Postives = 100/218 (45.87%), Query Frame = 2
Query: 818 VQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLY--FEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN 1465
V+ LL GA ++ ++ GL LH AC + ++ K L+++GA ++ D +T LH AAS G E+ L+ GAD +N + PL+LA +++ +LL E++G + + + E K ML + + NC IN T + +H S + +I LL+ G +N + +PLH A D +LL N
Sbjct: 65 VKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEE----KIMLNDAQSWLNKNC---------INESKDKTEASALHVASSKGYL-----KVIGLLLQLGV--EINAKDADNWTPLHAAAHWGQKDACELLSEN 262 HSP 4 Score: 63.929 bits (154), Expect = 9.891e-11 Identity = 37/101 (36.63%), Postives = 55/101 (54.46%), Query Frame = 2
Query: 2489 LLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAI 2791
LL +GAD+N Q+ GL LH A + D+ +L+ A+++ D G+T LH AA ++ L+ +GAD N EG+ PLDL D+ LL + I
Sbjct: 68 LLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEI 168 HSP 5 Score: 56.6102 bits (135), Expect = 2.138e-8 Identity = 63/267 (23.60%), Postives = 122/267 (45.69%), Query Frame = 2
Query: 1271 FTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV-RNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDK 2068
F + + +SS +V +K LL +GA +N N G + LH A + +N DL+++LV + +++ EG T +LH AA S I L+ G++ N+G L + D+ + L+++ ++ + D+ + + K+ N+ +S + + N+ + + + AS G ++++ +L+ V +N +D D + TPLH AA + + ++L ENGAD +A++K
Sbjct: 47 FNSGIVFLSAVSSGDVDEVKK-----LLAKGA--DINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWT---SLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDE--IENQGIDVDDARAV----EEKIMLND-------------------AQSWLNKNCINESKDKTEASALHVASSKGYLKVIGLLLQ-----LGVEINAKDAD--NWTPLHAAAHWGQKDACELLSENGADFNARNK 271 HSP 6 Score: 52.373 bits (124), Expect = 6.074e-7 Identity = 29/68 (42.65%), Postives = 40/68 (58.82%), Query Frame = 2
Query: 395 KDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKN 598
KD+ LH A S G+ V+ +L+ G + NAKD N+TPLH AA G+ D C +L + AD N +N
Sbjct: 203 KDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDACELLSENGADFNARN 270 HSP 7 Score: 50.8322 bits (120), Expect = 1.927e-6 Identity = 62/251 (24.70%), Postives = 110/251 (43.82%), Query Frame = 2
Query: 1418 AILTKNVDLIQ-LLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVL-VNC--RDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNV 2158
A+ + +VD ++ LL + +IN ++G+T ALH A + + +LV+ G++ + N+G+ + C + +YL A ++ N EGE+ P++ +E+ D +E Q ++ A VE +I+ N D +S L NC D ++ LH A+ + V+ +LL+ G +++AKD PLH A +G ELL + A+ N
Sbjct: 56 AVSSGDVDEVKKLLAKGADINYQNVDGLT---ALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAAS---CGSVEIAKYLIENGADVAAVNN-------------------------EGEL--PLD-LAEEEDMEDLLTDEIENQGIDVDDARAVEE--KIMLN--DAQSWLNKNCINESKDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDACELLSENGADFNA 268
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Nematostella
Match: EDO36720 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHV7]) HSP 1 Score: 90.8929 bits (224), Expect = 5.294e-20 Identity = 75/240 (31.25%), Postives = 114/240 (47.50%), Query Frame = 2
Query: 305 RKSTPLHFASGFGRKEMVE--FLIS---RGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLE------AARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEA 991
RK++ F RK++V FL+ R NV + DE GL L AC G+ +V++L+S G++ + +D ++ LH AAV G + L+ ADV+++N G D+ATD + +V K+ L + A R L+ I L N + + + LH AG VQ LLE GA+V+ + G PLH A G+I+ LL GA + A + P+ A
Sbjct: 7 RKTSSSIFHDAVARKDIVRLAFLMESNRRQFNVDLLDEDGLTALQRACFTGNLKLVQLLVSYGANLDIQDKEGWSVLHAAAVAGNLSILRYLVAVGADVSVRNDLGELPIDVATDVHCVIVLAEGMKRAGLRKLTDKFFAKRPQLKTLIKDSLIQGLDNVNDLNDSGISLLHEMAGRGDSESVQFLLEHGAEVNRQSLNGSSPLHEAVQSGNIDCTLLLLRSGADLYAETDGGFLPIDIA 246 HSP 2 Score: 90.1225 bits (222), Expect = 8.494e-20 Identity = 70/238 (29.41%), Postives = 106/238 (44.54%), Query Frame = 2
Query: 1976 STPLHFAAGFNRISVVKVLLENGA---DVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNI-------NCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQ 2659
S+ H A I + L+E+ +V D+ GL L AC G+ K+ +LL+ Y AN+++ D ++ LH AA G I + L+ AD RN G P+D+ + + A L AK +L T + N D + LH +G + E +FLLE GA+VN Q G PLH A G+ D LLL+ A++ + G+ P+ A +
Sbjct: 11 SSIFHDAVARKDIVRLAFLMESNRRQFNVDLLDEDGLTALQRACFTGNLKLVQLLVSYGANLDIQDKEGWSVLHAAAVAGNLSILRYLVAVGADVSVRNDLGELPIDVATDVHCVIVLAEGMKRAGLRKLTDKFFAKRPQLKTLIKDSLIQGLDNVNDLNDSGISLLHEMAGRGDSESVQFLLEHGAEVNRQSLNGSSPLHEAVQSGNIDCTLLLLRSGADLYAETDGGFLPIDIARE 248 HSP 3 Score: 58.5362 bits (140), Expect = 4.183e-9 Identity = 60/212 (28.30%), Postives = 94/212 (44.34%), Query Frame = 2
Query: 860 DKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKR----------MLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN 1465
D+ GL L AC G++++ + L+ GA ++ D ++ LH AA G + L+A GAD + N + P+++A TD ++ E G +L + KR ++Q L N ND N S I+ +H G ++FLL GA + LN GSSPLH A+ + N+D LL+R+
Sbjct: 43 DEDGLTALQRACFTGNLKLVQLLVSYGANLDIQDKEGWSVLHAAAVAGNLSILRYLVAVGADVSVRNDLGELPIDVA-TDVHCVIVLAE--GMKRAGLRKLTDKFFAKRPQLKTLIKDSLIQGLDNVNDLNDSG-------ISLLHEMAG--------------RGDSESVQFLLEHGAEVNRQSLN--GSSPLHEAVQSGNIDCTLLLLRS 228 HSP 4 Score: 56.9954 bits (136), Expect = 1.441e-8 Identity = 36/126 (28.57%), Postives = 63/126 (50.00%), Query Frame = 2
Query: 2432 NSTPLHLASGYNNLEIAEFLLE---QGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQT 2800
+S+ H A ++ FL+E + +V+ D+ GL L A G+ + LL+ Y AN++ DK G++ LH AA + L+A GAD ++N G+ P+D+ + +L E + +
Sbjct: 10 SSSIFHDAVARKDIVRLAFLMESNRRQFNVDLLDEDGLTALQRACFTGNLKLVQLLVSYGANLDIQDKEGWSVLHAAAVAGNLSILRYLVAVGADVSVRNDLGELPIDVATDVHCVIVLAEGMKRA 135 HSP 5 Score: 51.6026 bits (122), Expect = 7.864e-7 Identity = 42/176 (23.86%), Postives = 78/176 (44.32%), Query Frame = 2
Query: 197 EENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFG-------------------------RKSTPLHFASGFGRKEMV-EFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK 646
+E L A +L +++ L+ +G DV+ +++ G RK T FA K ++ + LI NV+ ++ G+ LH G ++ V+ L+ G++ N + + +PLHEA G D ++LL++ AD+ + G D+A +N +
Sbjct: 77 KEGWSVLHAAAVAGNLSILRYLVAVGADVSVRNDLGELPIDVATDVHCVIVLAEGMKRAGLRKLTDKFFAKRPQLKTLIKDSLIQGLDNVNDLNDSGISLLHEMAGRGDSESVQFLLEHGAEVNRQSLNGSSPLHEAVQSGNIDCTLLLLRSGADLYAETDGGFLPIDIARENTIR 252
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Nematostella
Match: EDO49485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG84]) HSP 1 Score: 88.9669 bits (219), Expect = 1.306e-19 Identity = 75/242 (30.99%), Postives = 111/242 (45.87%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIV-LLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAM 946
AC + K LL G DVN+KDN R TPLH A+ G +E L+S+ + + D+ G PLH A + V L++AGS+ N D TPLH AA C+ LL+ + + + ++ G +A A+ N KL + LL+ + N L +S TPLHLAA + +++L++ + +D G PL A GH+E + LL AM
Sbjct: 7 ACLGGFTECCKKLLFYGVDVNAKDNNDR--TPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYASANGHKLAI------EMLLDVSANDLLNR-------------SSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLLNHNAM 227 HSP 2 Score: 71.2478 bits (173), Expect = 1.500e-13 Identity = 70/255 (27.45%), Postives = 110/255 (43.14%), Query Frame = 2
Query: 1859 MQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEIC-KLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNS-----TPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSA 2605
MQ L + G C+ L V VN +D + R TPLH AA ++ +++L+ D + DK G PLH A + + + L+ +NVN D TPLH AAA C + LL+ + KR+K G++ + G ++ L ++++ D R+S TPLHLA+ + + E L++ D+ +D G PL AS GH + LL ++A
Sbjct: 1 MQPLHLACLGGFTECCKKLL----FYGVDVNAKDNNDR--TPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYAS--------------------ANGHKLAIEML---LDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLLNHNA 226 HSP 3 Score: 70.4774 bits (171), Expect = 2.769e-13 Identity = 70/232 (30.17%), Postives = 101/232 (43.53%), Query Frame = 2
Query: 2075 LVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTS-VNINCRDTQGRNSTPLHLASGYN-NLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANV---NSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
+ PLH AC G + + L+ Y +VN D TPLH AA G +LL+ D + +K G PL A V LVT+ N+N DT+ TPLH A+ + + + E LL + + +DK G +H AS+ GH +LL SAN S+ TPLH AA +L+ D ++++ G++PLDL S
Sbjct: 1 MQPLHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYA--------------------AGHTNFRCVMSLVTAGSNVNKPDTKA--CTPLHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLAS 210 HSP 4 Score: 63.5438 bits (153), Expect = 6.519e-11 Identity = 67/234 (28.63%), Postives = 98/234 (41.88%), Query Frame = 2
Query: 416 PLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYN-RCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQ-------YTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA 1093
PLH AC G + K L+ G D NAKD + TPLH AA G + +L+ D N + G + L AA + +M+++T N + D + TPLH AA + + V+ LL +DK G +H A + GH + LL+ V+A DL TPLH AA G +L+ D + ++PL+LA+
Sbjct: 3 PLHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLG---------------------RNPLHYAAGHTNFRCVMSLVTA-GSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYASANGHKLAIEMLLD----VSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLAS 210 HSP 5 Score: 60.077 bits (144), Expect = 7.557e-10 Identity = 43/133 (32.33%), Postives = 63/133 (47.37%), Query Frame = 2
Query: 2387 LVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLC-SLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLE 2782
L V++N +D R TPLH A+ N+ E L+ Q D N DK G PLH A+ + + L+ +NVN D TPLH AA + C LL + +A ++ +G S + SA + +E
Sbjct: 20 LFYGVDVNAKDNNDR--TPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYASANGHKLAIE 150 HSP 6 Score: 58.151 bits (139), Expect = 3.863e-9 Identity = 36/109 (33.03%), Postives = 54/109 (49.54%), Query Frame = 2
Query: 2441 PLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADV 2767
PLHLA E + LL G DVNA+D PLH A+ G+ + LL+ + + N DK G PLH AA + L+ G++ + + +PL +A+D+
Sbjct: 3 PLHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDL 111 HSP 7 Score: 56.6102 bits (135), Expect = 1.369e-8 Identity = 38/115 (33.04%), Postives = 51/115 (44.35%), Query Frame = 2
Query: 674 QELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVC 1018
Q L A G E +L + V+ +A D TPLH AA ++LL+ + DK G PLH A + + SL+ G+ VN PD TPLH AA+ C
Sbjct: 2 QPLHLACLGGFTECCKKLLF-YGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKC 115
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Nematostella
Match: EDO38725 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC11]) HSP 1 Score: 83.1889 bits (204), Expect = 8.781e-18 Identity = 77/254 (30.31%), Postives = 107/254 (42.13%), Query Frame = 2
Query: 281 VNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGA 1042
+N D+ G + LH A+ + R E + LI G SI+D PLH AC G +V ++L+ G+ + K+ TPLH AA G D LL ++ ++ T F L A G E M +L D + +PLHLA R V+ L G + +D+ G LH A G +V LL GA +NA D ++TPL AA G + C LL GA
Sbjct: 1 LNKMDDTG--FSALHHATRYNRVEAMAELIRNG---SIEDLS--TPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPF---------------------LSAVAAGRTENAMLLLE--------KDENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVKTCMELLQSGA 218 HSP 2 Score: 77.411 bits (189), Expect = 1.070e-15 Identity = 66/244 (27.05%), Postives = 98/244 (40.16%), Query Frame = 2
Query: 1979 TPLHFAAGFNRISVVKVLLENGA--DVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGA 2704
+ LH A +NR+ + L+ NG+ D+ PLH AC G +V LL++ A ++ + TPLH AA G F+ K LL +R+ TP ++ V T + + +PLHLA E L G N +D+ G LH A+ G + LL+Y A++N+ D +TPL AA+ + C LL GA
Sbjct: 10 SALHHATRYNRVEAMAELIRNGSIEDLS-------TPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPF----------------------------LSAVAAGRTENAMLLLEKDENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVKTCMELLQSGA 218 HSP 3 Score: 62.003 bits (149), Expect = 1.688e-10 Identity = 39/110 (35.45%), Postives = 52/110 (47.27%), Query Frame = 2
Query: 773 STPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGAD---PTHLNCHNKSPLELAA 1093
STPLH+A + +LL+E GA + K+ G PLH A G + K LL +G ++ D TP A + GRTE LLL + P HL C L + A
Sbjct: 37 STPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEKDENQKSPLHLACAAGRALHVEA 146 HSP 4 Score: 61.6178 bits (148), Expect = 2.528e-10 Identity = 36/93 (38.71%), Postives = 48/93 (51.61%), Query Frame = 2
Query: 308 KSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADV 586
+ +PLH A GR VE L G + + +DE G LH A G VV L+ G+D NA+D +TPL AA G C+ LLQ+ A +
Sbjct: 128 QKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVKTCMELLQSGASI 220 HSP 5 Score: 60.4622 bits (145), Expect = 5.453e-10 Identity = 66/232 (28.45%), Postives = 96/232 (41.38%), Query Frame = 2
Query: 398 DEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAA 1093
D+ G LH+A + + + LI GS + TPLH A KG +V +L++ A ++ KN G T +A G + + L + ++E + TPF +AAG R LLLE KD+ PLH AC+ G ++L G N D + LH AA G +V + LL YGAD + + +PL AA
Sbjct: 5 DDTGFSALHHATRYNRVEAMAELIRNGSIEDLS-----TPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMA-------ACIGSFDALKFLLSKGVAIDERDFSHRTPFLSA-------------VAAG--RTENAMLLLE-------KDENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAA 202 HSP 6 Score: 55.8398 bits (133), Expect = 1.815e-8 Identity = 41/127 (32.28%), Postives = 55/127 (43.31%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNA 2245
L A AG E +LE + + KS PLH A R V+ L G + +D+ G LH A G KV L++Y A++N D ++TPL AA G + C LLQ A
Sbjct: 106 FLSAVAAGRTENAMLLLEKDENQKS--------------PLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVKTCMELLQSGA 218 HSP 7 Score: 55.0694 bits (131), Expect = 3.325e-8 Identity = 40/121 (33.06%), Postives = 59/121 (48.76%), Query Frame = 2
Query: 2435 STPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQ 2797
STPLH+A ++E+ L+E+GA ++ ++ G PLH A+ G D LL ++ D TP A RT+ LLL K+ +SPL L AA R L EA+++
Sbjct: 37 STPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLE-------KDENQKSPLHLACAAG-RALHVEALTR 149
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Nematostella
Match: EDO39113 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB41]) HSP 1 Score: 81.2629 bits (199), Expect = 2.083e-17 Identity = 65/205 (31.71%), Postives = 95/205 (46.34%), Query Frame = 2
Query: 341 GRKEMVEFLISRG--ANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAK---DVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERG 940
GRK+ V L+ RG N++ D G PLH A + H D+V++L+ AG+D + + + P+ AA + C VLL+A ADVN+ N G TA AT R G + + +L ++ + D +T LH+AA I++ LL GA + AKD G H A G EV ++LL G
Sbjct: 6 GRKDCVRKLL-RGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHAT---------------------RRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLRTG 188 HSP 2 Score: 70.4774 bits (171), Expect = 1.253e-13 Identity = 55/171 (32.16%), Postives = 83/171 (48.54%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFG-RKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGS-DPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTIL 730
A N + +V+ LLD G DV+ + + ++ P+ A+ F E L+ GA+V+I + G LH+A GH+ +V++L+ G D N D N T LH AA+ G + LL A++ K++DG TAF L AAR G EE + T+L
Sbjct: 36 ATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHL---------------------AAREGEEEVLQTLL 185 HSP 3 Score: 66.6254 bits (161), Expect = 2.237e-12 Identity = 53/141 (37.59%), Postives = 74/141 (52.48%), Query Frame = 2
Query: 1919 NNSDIKSVLVNC-RDTDGRHST------PLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQ-YNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDV 2314
N+ DI +L++ D D R S P+ AA FN I +VLL GADV+ + G LH+A GH+K+ ELL++ +VNV D T LH AA G I K LL A+ ++ DG T L +EG+++V
Sbjct: 40 NHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEV 180 HSP 4 Score: 60.077 bits (144), Expect = 3.922e-10 Identity = 52/186 (27.96%), Postives = 88/186 (47.31%), Query Frame = 2
Query: 686 EAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLV---PLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYG-ADPTHLNCHNKSPLELAA---TDELRELLYF--------EHKGFVLFNY-CRLGEYNKVKRMLQ 1195
+A R+G ++ + +L N + D +PLH A N IVQ+LL+ GA V + + P+ A + IE + LL GA VN + + T LH A +G +++ LLL G D ++ N + L +AA ++ + + L F ++ GF F+ R GE ++ +L+
Sbjct: 1 QACRDGRKDCVRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLR 186 HSP 5 Score: 58.9214 bits (141), Expect = 1.042e-9 Identity = 50/196 (25.51%), Postives = 86/196 (43.88%), Query Frame = 2
Query: 2018 VVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNV---CDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLK 2596
V K+L N +++ D G PLH A + H + ++L+ A+V+V + + P+ AA E C++LL+ AD + N G T L A ++G V+ L+ ++ N+T LH+A+ + I + LL GA++ A+D G H A+ G ++ LL+
Sbjct: 11 VRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTAL--------------------HHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLR 186 HSP 6 Score: 58.151 bits (139), Expect = 1.807e-9 Identity = 56/223 (25.11%), Postives = 83/223 (37.22%), Query Frame = 2
Query: 2093 ACSYGHAKVAELLIQYN-ANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLK-YSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
AC G L++ N N+N D F+PLH A +I ++LL AD R +Q++ P+ A+ +N +E LL GADVN + G LH+A+ GH+ M LLL+ +VN D+ T LH AA + LL GA+ K+ +G + L +
Sbjct: 2 ACRDGRKDCVRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGS-----------------------------------PSIQRI----------------PPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAA 173 HSP 7 Score: 55.8398 bits (133), Expect = 1.319e-8 Identity = 48/180 (26.67%), Postives = 79/180 (43.89%), Query Frame = 2
Query: 314 TPLHFASGFGRKEMVEFLISRGANVSIKDEGG---LIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQ-AKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMG 841
+PLH+A+ ++V+ L+ GA+V ++ + P+ A F + +VL+ AG+D N + + T LH A +G + + +LL+ + DVN+ + D T+ + AA G I T+L N A D T HLAA ++Q LL G
Sbjct: 31 SPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHV---------------------AAMIGSNPIIKTLLF-CGANITAKDNDGFTAFHLAAREGEEEVLQTLLRTG 188
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Medaka
Match: ENSORLT00000038944.1 (tankyrase-1 [Source:NCBI gene;Acc:101158122]) HSP 1 Score: 1116.68 bits (2887), Expect = 0.000e+0 Identity = 627/1278 (49.06%), Postives = 837/1278 (65.49%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFS-SNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSN-----------NNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPS 3991
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++V+ L+ GANV +D+GGLIPLHNACSFGH++VV +L+ G++PNA+D WNYTPLHEAA+KGK DVCIVLLQ AD N++N+DG +A DLA + + K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH+KS +++A T EL+E L +E KG L R + K K+ L + ++INF HP T +T +H ++S + KRK + E LLR+GA ++N NK +PLH+A + D++++L ++ ++N +D L G+ ALH AA C +L+ YG++ ++ + QG+ A+Q+ + A N+ VP S + + E G++ ++S + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+TPLDLVK+GD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRDTQGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+PLDL +A D+R LL +A+ A A + ++ +V + + + + S +C + ++ + + L A SN N+ L++++ +FL+S+ L+ L ++F +EQ+++D+L +MGH ELKE+G+ AYGHRHK++KGI + G++P + H +S T+L+DL P D ++QSVEE++QS+IR H+D N GG+F+RY I+K++++ N+KL ++Y R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCY+C RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P P+
Sbjct: 131 RELFEACRNGDVSRVKKLVD-AMNVNAKDMAGRKSTPLHFAAGFGRKDVVDHLLQMGANVHARDDGGLIPLHNACSFGHSEVVSLLLCQGAEPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-EPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHSKSSVDMAPTPELKERLTYEFKGHSLLQAAREADMAKAKKTLAL----------------EIINFKHPHTHETALHCAVASPH--PKRKQVTELLLRKGA--NVNEKNKDFMTPLHVAAERAHNDIMEVLQKHGAKVNALDTL----GQTALHRAALAGHLQTCRLLLGYGADASLVSLQGFTAAQMGNE-----------------AVQQILNESVPVRNSDV-------DYRLLEAAKAGDL-DTIKSLCTPQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPDNINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPDALPSCLKPQATVVSAGVVSTGATAAVVISPSPSPSCLSAASSIDNLTAPLSDIAGAGVSNVADGASGSDRKENDTLLDMTINQFLKSLGLEHLRDIFQREQISLDVLADMGHEELKEIGINAYGHRHKLIKGIERL---------LGGQQGANPYLTFHCSS--------------------------------QGTVLIDLAPDDKEFQSVEEELQSTIREHRDGGNAGGVFSRYNIIKIQKVVNKKLRERYAHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYVCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIGKPESVAAPA 1276
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Medaka
Match: tnksb (tankyrase-1-like [Source:NCBI gene;Acc:105353570]) HSP 1 Score: 1110.52 bits (2871), Expect = 0.000e+0 Identity = 625/1266 (49.37%), Postives = 837/1266 (66.11%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXX-XXXXXXXRLRTDGDANTSNNNN------CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHE+A+KGK DVCIVLLQ AD N++N+DG +A DLA D++ K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH+KS +++A T EL+E L +E KG L R + KVK+ L + ++I F HP T +T +H ++S + KRK + E LLR+GA ++N NK +PLH++ + D++++L ++ ++N +D L G+ ALH AA C +L+++G++ I + QG+ A+Q+ + + + L+E VPT S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+ PLD+VK+GD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRD QGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+ LDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + T G A+ + ++ L++++++FL+S+ L+ L ++F +EQ+T+D+L++MGH ELKE+G+ AYGHRHK++KG+ + G++P + H C+ + T+L+DL P D +YQSVEE+MQS+IR H+D N GG+F+RY I+K++++ N+KL ++ R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCY+C RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 114 RELFEACRNGDVSRVKRLVDT-VNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHESAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DSSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRMEVCSLLLSHGADPTLLNCHSKSAVDMAPTPELKERLTYEFKGHSLLQAAREADIAKVKKTLAL----------------EVIGFKHPQTNETALHCAVASPH--PKRKQVTELLLRKGA--NINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTL----GQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEA-VQQILNEN----------------VPTRNSDV-------DYRFLEAAKAGDL-DTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSQENINCRDAQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVVSASVI--SPASTPSCLSAASSIDNLAGPLTELAAAAAGTSGVADGATGSDRKEGEMAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLVDMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGANPYLTFH---CA-----------------------------NQGTILIDLSPDDKEYQSVEEEMQSTIREHRDGGNAGGVFSRYNIIKIQKVVNKKLRERCAHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQILKP 1245 HSP 2 Score: 256.144 bits (653), Expect = 4.265e-69 Identity = 178/518 (34.36%), Postives = 263/518 (50.77%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + D+ VK L + +V + T LH A RK++ E L+ +GAN++ K++ + PLH + H DV++VL G+ NA D T LH AA+ G C +LL AD + + G TA + + +++ + LEAA+ G +T+ + TP NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N PL++ ++++LL + L + + G +V Q L + + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G A L T D+ L + D +C +V
Sbjct: 415 TYEFKGHSLLQAAREADIAKVKKTLAL--EVIGFKHPQTNETALHCAVASPHPKRKQVTELLLRKGANINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTLGQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEAVQQILNENVPTRNSDVDYRFLEAAKAGDLDTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCSQENINCRDAQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVV 902
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Medaka
Match: tnksb (tankyrase-1-like [Source:NCBI gene;Acc:105353570]) HSP 1 Score: 1105.12 bits (2857), Expect = 0.000e+0 Identity = 625/1273 (49.10%), Postives = 837/1273 (65.75%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKST-------PLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXX-XXXXXXXRLRTDGDANTSNNNN------CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHE+A+KGK DVCIVLLQ AD N++N+DG +A DLA D++ K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKST PLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH+KS +++A T EL+E L +E KG L R + KVK+ L + ++I F HP T +T +H ++S + KRK + E LLR+GA ++N NK +PLH++ + D++++L ++ ++N +D L G+ ALH AA C +L+++G++ I + QG+ A+Q+ + + + L+E VPT S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+ PLD+VK+GD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRD QGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+ LDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + T G A+ + ++ L++++++FL+S+ L+ L ++F +EQ+T+D+L++MGH ELKE+G+ AYGHRHK++KG+ + G++P + H C+ + T+L+DL P D +YQSVEE+MQS+IR H+D N GG+F+RY I+K++++ N+KL ++ R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCY+C RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 114 RELFEACRNGDVSRVKRLVDT-VNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHESAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DSSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTSQKMLSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRMEVCSLLLSHGADPTLLNCHSKSAVDMAPTPELKERLTYEFKGHSLLQAAREADIAKVKKTLAL----------------EVIGFKHPQTNETALHCAVASPH--PKRKQVTELLLRKGA--NINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTL----GQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEA-VQQILNEN----------------VPTRNSDV-------DYRFLEAAKAGDL-DTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSQENINCRDAQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVVSASVI--SPASTPSCLSAASSIDNLAGPLTELAAAAAGTSGVADGATGSDRKEGEMAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLVDMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGANPYLTFH---CA-----------------------------NQGTILIDLSPDDKEYQSVEEEMQSTIREHRDGGNAGGVFSRYNIIKIQKVVNKKLRERCAHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQILKP 1252 HSP 2 Score: 256.529 bits (654), Expect = 3.756e-69 Identity = 178/518 (34.36%), Postives = 263/518 (50.77%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + D+ VK L + +V + T LH A RK++ E L+ +GAN++ K++ + PLH + H DV++VL G+ NA D T LH AA+ G C +LL AD + + G TA + + +++ + LEAA+ G +T+ + TP NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N PL++ ++++LL + L + + G +V Q L + + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G A L T D+ L + D +C +V
Sbjct: 422 TYEFKGHSLLQAAREADIAKVKKTLAL--EVIGFKHPQTNETALHCAVASPHPKRKQVTELLLRKGANINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTLGQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEAVQQILNENVPTRNSDVDYRFLEAAKAGDLDTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCSQENINCRDAQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVV 909
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Medaka
Match: tnksb (tankyrase-1-like [Source:NCBI gene;Acc:105353570]) HSP 1 Score: 1101.66 bits (2848), Expect = 0.000e+0 Identity = 625/1273 (49.10%), Postives = 837/1273 (65.75%), Query Frame = 2
Query: 209 RTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKST-------PLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXX-XXXXXXXRLRTDGDANTSNNNN------CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENP 3970
R LF+AC+N D+ VK L+D +VN+KD GRKSTPLHFA+GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHE+A+KGK DVCIVLLQ AD N++N+DG +A DLA D++ K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKST PLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH+KS +++A T EL+E L +E KG L R + KVK+ L + ++I F HP T +T +H ++S + KRK + E LLR+GA ++N NK +PLH++ + D++++L ++ ++N +D L G+ ALH AA C +L+++G++ I + QG+ A+Q+ + + + L+E VPT S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+ PLD+VK+GD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRD QGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYGH D+AALL+KY+ VN+ DKW +TPLHEAAQK RTQLC+LLLAHGAD +KN EGQ+ LDL +A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + T G A+ + ++ L++++++FL+S+ L+ L ++F +EQ+T+D+L++MGH ELKE+G+ AYGHRHK++KG+ + G++P + H C+ + T+L+DL P D +YQSVEE+MQS+IR H+D N GG+F+RY I+K++++ N+KL ++ R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCY+C RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQAYP +LITY I P
Sbjct: 97 RELFEACRNGDVSRVKRLVDT-VNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHESAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DSSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTSQKMLSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRMEVCSLLLSHGADPTLLNCHSKSAVDMAPTPELKERLTYEFKGHSLLQAAREADIAKVKKTLAL----------------EVIGFKHPQTNETALHCAVASPH--PKRKQVTELLLRKGA--NINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTL----GQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEA-VQQILNEN----------------VPTRNSDV-------DYRFLEAAKAGDL-DTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSQENINCRDAQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVVSASVI--SPASTPSCLSAASSIDNLAGPLTELAAAAAGTSGVADGATGSDRKEGEMAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLVDMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGANPYLTFH---CA-----------------------------NQGTILIDLSPDDKEYQSVEEEMQSTIREHRDGGNAGGVFSRYNIIKIQKVVNKKLRERCAHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQILKP 1235 HSP 2 Score: 256.144 bits (653), Expect = 4.406e-69 Identity = 178/518 (34.36%), Postives = 263/518 (50.77%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVR-NEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIV 1711
++E +L A + D+ VK L + +V + T LH A RK++ E L+ +GAN++ K++ + PLH + H DV++VL G+ NA D T LH AA+ G C +LL AD + + G TA + + +++ + LEAA+ G +T+ + TP NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N PL++ ++++LL + L + + G +V Q L + + NC + + T +H NN+ + E+LL GA +N +K G PLH A +VD+ LL++ N +N T LH AA+ + +C +L+ +G++ T+ +G A L T D+ L + D +C +V
Sbjct: 405 TYEFKGHSLLQAAREADIAKVKKTLAL--EVIGFKHPQTNETALHCAVASPHPKRKQVTELLLRKGANINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTLGQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEAVQQILNENVPTRNSDVDYRFLEAAKAGDLDTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCSQENINCRDAQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFT---PLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVV 892
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Medaka
Match: tnksb (tankyrase-1-like [Source:NCBI gene;Acc:105353570]) HSP 1 Score: 630.943 bits (1626), Expect = 0.000e+0 Identity = 367/756 (48.54%), Postives = 484/756 (64.02%), Query Frame = 2
Query: 335 GFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEIN-IDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVE-EYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLL 2593
GFGRK++VE L+ GANV +D+GGLIPLHNACSFGHA+VV +L+ G+DPNA+D WNYTPLHE+A+KGK DVCIVLLQ AD N++N+DG +A DLA D++ K V TGEYKK ELLEAAR+G EE +M +LTP NVNCHASDGRKSTPLHLAAGYNR RIVQLLL+ GA VHAKDKGGLVPLHNACSYGH EV + LL+ GA VNA DLWQ+TPLHEAASK R EVCSLLL++GADPT LNCH+KS +++A T EL+E L +E KG L R + KVK+ L + ++I F HP T +T +H ++S + KRK + E LLR+GA ++N NK +PLH++ + D++++L ++ ++N +D L G+ ALH AA C +L+++G++ I + QG+ A+Q+ + A N+ VPT S + + E G++ V+ + N + +E + + A+ V +V +L + +D V+ +D G PLH A + V ++L+ +GA V+ D PLH A + G K+EICKLLL++ ADP K+N+DG+ PLD+VK+GD D+ DLL D+ALL+AAKKG +A+VQKL + NINCRD QGRNSTPLHLA+GYNNLE+AE+LLE GADVNAQDKGGLIPLHNA+SYG D+ ALL+
Sbjct: 3 GFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHESAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLA-DSSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRMEVCSLLLSHGADPTLLNCHSKSAVDMAPTPELKERLTYEFKGHSLLQAAREADIAKVKKTLAL----------------EVIGFKHPQTNETALHCAVASPH--PKRKQVTELLLRKGA--NINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTL----GQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNE-----------------AVQQILNENVPTRNSDV-------DYRFLEAAKAGDL-DTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGAD-----VHAKDKGGL--VPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKG---------------------------------KYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSQENINCRDAQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGADDIRALLM 668 HSP 2 Score: 545.428 bits (1404), Expect = 3.268e-173 Identity = 400/1155 (34.63%), Postives = 578/1155 (50.04%), Query Frame = 2
Query: 797 GYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEF--LSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGF---NRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXX------XXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLI---------------------------------PLHNASSYGHADMAALLLKYSA----------------------------------------------------NVNSADKWGY--TPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS---AADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXX-XXXXXXXRLRTDGDANTSNNNN------CSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKD--NVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQ 3928
G+ R +V+ LL+ GA VHA+D GGL+PLHNACS+GH EV LL +GA NA D W YTPLHE+A KG+ +VC +LL +GADP N KS L+LA + + +L E+K L R G K+ +L L + NC HA+ + + S+PLHLA V ++QLL+++ +++ G+ LH A + +L+ +G+ +DL +F L E + C S L ++ + K ++AP E +KE+ T E +L+A++ D+ V + L ++ + T+ T LH A R V ++LL GA+++ K+K + PLH + H V E+L ++ A VN D T LH AA G + CKLLL + AD + G T + E Q + + + EAAK G + VQ+L T N+NCRD +GR+STPLH A+GYN + + E+LL GADV+A+DKGGL+ PLH A++ G ++ LLLK+ A N+N D G TPLH AA N ++ LL HGAD ++ G PL + A D+R LL +A+ A A + ++ ++ S + P+ L+ + + N + + T G A+ + ++ L++++++FL+S+ L+ L ++F +EQ+T+D+L++MGH ELKE+G+ AYGHRHK++KG+ + G++P + H C+ + T+L+DL P D +YQSVEE+MQS+IR H+D N GG+F+RY I+K++++ N+KL ++ R++E+ +EN N NER+LFHGSPFI AI+++GFDERHAYIGGMFGAGIYFAENSSKSNQYVYG+GGGTGC HKDRSCY+C RQML CRV LGK+FLQ + +KMAHAPPGHHSV+GRPSV GL+YAEYVIYRGEQ
Sbjct: 3 GFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHESAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADSSA-KAVLTGEYKKDELLEAARSGNEEKLMALLTPL----NVNC----------------------HAS-----------------------------DGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLV---PLHNACSYGHYEVTELLLKHGA--------------CVNAMDLWQFTPLHEAASKNRMEVC------------SLLLSHGADPTLLNCHSKSAVDMAPTPE--LKERLT----YEFKGHSLLQAAREADIAKVKKTLA----LEVIGFKHPQTN---ETALHCAVASPHPKRKQVTELLLRKGANINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTLGQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEAVQQILNENVPTRNSDVDYRFLEAAKAGDLDTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSQENINCRDAQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGADDIRALLMDAMPPDALPSCFKPQATVVSASVI--SPASTPSCLSAASSIDNLAGPLTELAAAAAGTSGVADGATGSDRKEGEMAVLDMNISQFLKSLGLEHLRDIFEREQITLDVLVDMGHEELKEIGINAYGHRHKLIKGVERL---------LGGQQGANPYLTFH---CA-----------------------------NQGTILIDLSPDDKEYQSVEEEMQSTIREHRDGGNAGGVFSRYNIIKIQKVVNKKLRERCAHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQ 1016 HSP 3 Score: 231.491 bits (589), Expect = 1.118e-61 Identity = 157/433 (36.26%), Postives = 225/433 (51.96%), Query Frame = 2
Query: 191 SFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGF---GRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQ-----ELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELA--ATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLV 1459
++E +L A + D+ VK L + +V + T LH A RK++ E L+ +GAN++ K++ + PLH + H DV++VL G+ NA D T LH AA+ G C +LL AD + + G TA + + +++ + LEAA+ G +T+ + TP NVNC +GR STPLH AAGYNR +V+ LL GA VHAKDKGGLVPLHNACSYGH EVA+ L+ GA VN DLW++TPLHEAA+KG+ E+C LLL +GADPT N PL++ ++++LL + L + + G +V Q L + + NC + + T +H NN+ + E+LL GA +N +K G PLH A D+ LL+
Sbjct: 263 TYEFKGHSLLQAAREADIAKVKKTLAL--EVIGFKHPQTNETALHCAVASPHPKRKQVTELLLRKGANINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTLGQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEAVQQILNENVPTRNSDVDYRFLEAAKAGDLDTVQQLCTPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGD---AALLDAAKKGCLARV----QKLCSQENINCRDAQGRNS-----------TPLHLAAGYNNL-----EVAEYLLEHGA--DVNAQDKGGLIPLHNAASYGADDIRALLM 668 HSP 4 Score: 218.394 bits (555), Expect = 1.516e-57 Identity = 111/242 (45.87%), Postives = 156/242 (64.46%), Query Frame = 2
Query: 215 LFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKL--VFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLE 934
+A K DL V+ L +VN +D GR STPLHFA+G+ R +VE+L+ GA+V KD+GGL+PLHNACS+GH +V ++L+ G+ N D+W +TPLHEAA KGK ++C +LL+ AD KN DG D+ D +T + + G+ LL+AA+ G + + + N+NC + GR STPLHLAAGYN + + LLE GA V+A+DKGGL+PLHNA SYG ++ L++
Sbjct: 432 FLEAAKAGDLDTVQQLC-TPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGD---AALLDAAKKGCLARVQKLCSQENINCRDAQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGADDIRALLMD 669 HSP 5 Score: 207.608 bits (527), Expect = 4.729e-54 Identity = 161/518 (31.08%), Postives = 250/518 (48.26%), Query Frame = 2
Query: 110 NPNEYCTDIQDICDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGY---NRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLEL---AATDELRELLYFEHK--GFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRN-EEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDID 1636
+PN TD + D +AD+S + ++++ L +A ++ + + + LL +VN + GRKSTPLH A+G+ R +V+ L+ GA+V KD+GGL+PLHNACS+GH +V ++L+ G+ NA D+W +TPLHEAA K + +VC +LL AD L N +A D+A K T E+K LL+AAR + L V T LH A R ++ +LLL GA ++ K+K + PLH + H +V + L + GA VNA D T LH AA G + C LLL++GAD ++ + ++ A L E + + + + G+ + V Q L + NC + + T +H N V ++E+LL GA ++ +K G PLH A + ++ +LLVR+ +N+ L T LH AA IC +L+ +G++ T G + D D
Sbjct: 85 DPNIRNTDGKSALD-LADSSAKAVLTGEYKKD--ELLEAARSGNEEKLMALL-TPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRMEVCSLLLSHGADPTLLNCHSKSAVDMAPTPELKERLTYEFKGHSLLQAAREADIAKVKKTLA-LEVIGFKHPQTNETALHCAVASPHPKRKQVTELLLRKGANINEKNKDFMTPLHISAERAHNDVLEVLQKHGAKVNAVDTLGQTALHRAALAGHIQTCKLLLSFGADAAIVSLQGFTAAQMGNEAVQQILNENVPTRNSDVDYRFLEAAKAGDLDTV----QQLCTPQNVNCRDLEGRHS-----------TPLHFAAGYNRVA-----VVEYLLHHGA--DVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFT---PLHEAAAKGKYEICKLLLKHGADPTKKNRDGNIPLDMVKDGD 572 HSP 6 Score: 76.2554 bits (186), Expect = 2.060e-13 Identity = 41/94 (43.62%), Postives = 59/94 (62.77%), Query Frame = 2
Query: 212 TLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNA 493
L DA K L V+ L ++N +D GR STPLH A+G+ E+ E+L+ GA+V+ +D+GGLIPLHNA S+G AD ++ L+ P+A
Sbjct: 584 ALLDAAKKGCLARVQKLCS-QENINCRDAQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYG-ADDIRALLMDAMPPDA 675
BLAST of Poly [ADP-ribose] polymerase vs. Planmine SMEST
Match: SMESG000068744.1 (SMESG000068744.1) HSP 1 Score: 2584.67 bits (6698), Expect = 0.000e+0 Identity = 1340/1342 (99.85%), Postives = 1340/1342 (99.85%), Query Frame = 2
Query: 47 MNHEIYRNRSVSEGEVNCASYNPNEYCTDIQDICDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTXXXXXXXXXXXXXXXXMVFSSNRTLPTKLXXXXXXXXXXXXXXXXXXRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPXXXXXXXXXXXXXXXXDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDNVGGIFNRYTILKVERLCNQKLWQKYLIRKQXXXXXXXXXXXXXXXXHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQNNSENMDNLISLNHSGGTNNIDMDPE 4072
MNHEIYRNRSVSEGEVN ASYNPNEYCTDIQDICDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYDLLLSDSALLEAAKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTANNVISISSAAIPNSPMVFSSNRTLPTKLNNSINCTNHNNNSNGCSNRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPVTTSVLSSVFSSSVGSDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDNVGGIFNRYTILKVERLCNQKLWQKYLIRKQELFEENLNVVNERLLFHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQNNSENMDNLISLNHS GTNNIDMDPE
Sbjct: 1 MNHEIYRNRSVSEGEVNRASYNPNEYCTDIQDICDKIADTSLSKLTEASFEENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTKLVFTGEYKKQELLEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLVKEGDQDVYDLLLSDSALLEAAKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAADVRFLLEEAISQTANNVISISSAAIPNSPMVFSSNRTLPTKLNNSINCTNHNNNSNGCSNRLRTDGDANTSNNNNCSLEISVAEFLRSIRLDGLVELFYKEQVTMDILLEMGHPELKELGVVAYGHRHKILKGISKFNRSTSQAPSCENSLGSDPNVNIHSNSCSLHRKPGGGVGAIDPVTTSVLSSVFSSSVGSDKTLLVDLLPSDIDYQSVEEQMQSSIRPHKDNVGGIFNRYTILKVERLCNQKLWQKYLIRKQELFEENLNVVNERLLFHGSPFIQAIVNRGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGLGGGTGCQLHKDRSCYICPRQMLLCRVALGKAFLQLTGVKMAHAPPGHHSVVGRPSVAGLSYAEYVIYRGEQAYPLFLITYLIENPTKCLEPSQNNSENMDNLISLNHSDGTNNIDMDPE 1342
BLAST of Poly [ADP-ribose] polymerase vs. Planmine SMEST
Match: SMESG000000606.1 (SMESG000000606.1) HSP 1 Score: 231.106 bits (588), Expect = 9.297e-61 Identity = 223/870 (25.63%), Postives = 368/870 (42.30%), Query Frame = 2
Query: 200 ENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNN----TKLVFTGEYKKQELLEAARNGLEETIMTIL------TPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQK-------------LVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKY--SANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEG 2731
E+ + A + +L V L+ G D+N+ ++ G + LH A+ G +V+ LI R + + G LH A G +VVK+L+ G++ N + +TPL+ AA + +V L+ AD + DG T +A L+ + + ++ L A +++ + + + +VN +S G TPLH+AA Y + QLL+E A V+ + K + PLH A +G + + L++ GA+V+ TPLH +A G ++C L+ GAD ++ G + G++ R+L + + ++F+ T +H T N + + LL R + LN G +PLH+A + +++LL++ + E TG LH++A + S +L+ G+N T +G A L T NN+T+ V+ +K D + E + A+K G++EL+ L++ SD V RD + T LH AA R + +LL++GA + A+ K G PLH A Y VA +L+Q AN N TPLH A ++ +LLL ADP + K+G+TPL + K+ +QD+ +LL A A + + +Q L ++N + G S +HL + + + +A L+ GAD+ PLH A+ +G +MA L+ +N + G+TPLH AAQ+ QL +LLL AD +KN +G
Sbjct: 10 ESNHSFLRAARAGNLSKVIDFLETGTDINTCNDIGLSA--LHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVALQQGHERIVALLLENDNRSKQRLPALHIAVKKNDVNAVNLLLNSSEVDVNHQSSSG--FTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNITPLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKLIDNGAD----------------------VMSKTRNGLTSLHMAAQGDFEDTARILLLKRANINETTTDFL---------------TPLHVTAHYGN-----EKVARVLLDRRCDPNARALN--GFTPLHIACKKHKIKIVELLLKYGALIEATTE--TGLTPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLAT------------------------------------RNNQTET---------------VKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPN---VATRD----NYTALHVAAKEGRSEAISLLLDSGAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLASQEGHTDVVSLLLQHKADVNHKAKNGLTS--MHLTAQEDKVTVARILVHAGADITPTTTAEYTPLHTAAHFGQINMAKYLISLMDQNQINQKTQLGFTPLHLAAQQGHLQLVNLLLESRADPNIKNNQG 769 HSP 2 Score: 178.333 bits (451), Expect = 1.285e-44 Identity = 202/712 (28.37%), Postives = 306/712 (42.98%), Query Frame = 2
Query: 680 LLEAARNG-LEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNE-EINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGY---RASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFE--GEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-------------VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
L AAR G L + I + T ++N G + LHLAA IV+ L++ ++ A K G LH A G EV K L+E GA VN +TPL+ AA + EV + L+ GAD T +PL +A L H+ V +L N N S KQ+L +H + N+V N + LL +N + G +PLH+A N QLL+ + ++N +T LH+AA+ S + +L++ G+ + T G S + LCE L +D A +S + N T + + FE I + + E TT ++ + + G+ ++ +L+ D N R +G TPLH A ++I +V++LL+ GA + A + GL PLH + G + +L+Q AN+ + T LH A + E KLLL+ A P + ++ TPL + ++ G + +AL AAK+G+ + L+ S R TPLH+A+ Y+ + +A LL++ A+ NA K GL PLH A+ Y H +A LLL A+ K GYTPLH AA+KN L +LL AD+ ++ G SPL L S
Sbjct: 15 FLRAARAGNLSKVIDFLETGTDINTCNDIGLSA--LHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVA--------LQQGHERIVAL----------------LLENDNRS-------KQRL----------PALHIAVKKNDV-----NAVNLLLNSSEVD-VNHQSSSGFTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNIT---PLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKL-----IDNGADVMSKTR------------NGLTSLHMAAQGDFEDTARILLLKRANINETTT------DFLTPLHVTAHYGNEKVARVLLDRRCD-----PNARALNG--FTPLHIACKKHKIKIVELLLKYGALIEATTETGLTPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLATRNNQTETVKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPNVATRDNYTALHVAAKEGRSEAISLLLDS-GAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLAS 643 HSP 3 Score: 139.428 bits (350), Expect = 1.236e-32 Identity = 103/318 (32.39%), Postives = 154/318 (48.43%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYX--------XXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
L A++AG++ V LE +DI + C D + LH AA I +VK L++ + A K G LH A G +V ++L++ ANVN+ FTPL+ AA + E+ L+ AD +DG TPL + +++G + + AL A KK + V L+ S ++ TPLH+A+ Y N + A+ L+EQ ADVN Q K + PLH A+ +G + M LL+ A V+ + G TPLH +A+ QLC L+ +GAD K G + L + + D R LL
Sbjct: 15 FLRAARAGNLSKVIDFLETGTDINT----CNDIG---LSALHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVALQQGHERIVALLLENDNRSKQRLPALHIAVKKNDVNAVNLLLNSSEVDVNHQSSSGFTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNITPLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKLIDNGADVMSKTRNGLTSLHMAAQGDFEDTARILL 325 HSP 4 Score: 128.257 bits (321), Expect = 2.504e-29 Identity = 123/434 (28.34%), Postives = 190/434 (43.78%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQEL------LEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAAT-DELRELLYFEHKG-----------FVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLL 1456
ACK + +++V+ LL G + + G TPLH ++ G KE V L+ +GAN+ G LH A + VK+L+ AG+ P+AK N TPLH A G ++ + LQ+ +D N+ D TA +A L+ +G + E L A + + +IL N +A+ TPLH+A YN ++ QLLL+ A K K G PLH A + ++A LL A NA ++PL A+ +G T+V SLLL + AD H + + + L A D++ H G L G+ N K ++ ++ D N N K QL G T +H ++ L+ LL A N+ N QG + H+A +++ ++L
Sbjct: 378 ACKKHKIKIVELLLKYGALIEATTETGL--TPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLATRNNQTETVKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPNVATRDNYTALHVAAKEGRSEAISLLLDSGAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLASQEGHTDVVSLLLQHKADVNHKAKNGLTSMHLTAQEDKVTVARILVHAGADITPTTTAEYTPLHTAAHFGQINMAKYLISLM----DQNQIN--QKTQL--------GFTPLHLAAQQGHL-----QLVNLLLESRADP--NIKNNQGWTAAHIARKQHYLNIFEVL 788
BLAST of Poly [ADP-ribose] polymerase vs. Planmine SMEST
Match: SMESG000000606.1 (SMESG000000606.1) HSP 1 Score: 231.106 bits (588), Expect = 9.503e-61 Identity = 223/870 (25.63%), Postives = 368/870 (42.30%), Query Frame = 2
Query: 200 ENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNN----TKLVFTGEYKKQELLEAARNGLEETIMTIL------TPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQK-------------LVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKY--SANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEG 2731
E+ + A + +L V L+ G D+N+ ++ G + LH A+ G +V+ LI R + + G LH A G +VVK+L+ G++ N + +TPL+ AA + +V L+ AD + DG T +A L+ + + ++ L A +++ + + + +VN +S G TPLH+AA Y + QLL+E A V+ + K + PLH A +G + + L++ GA+V+ TPLH +A G ++C L+ GAD ++ G + G++ R+L + + ++F+ T +H T N + + LL R + LN G +PLH+A + +++LL++ + E TG LH++A + S +L+ G+N T +G A L T NN+T+ V+ +K D + E + A+K G++EL+ L++ SD V RD + T LH AA R + +LL++GA + A+ K G PLH A Y VA +L+Q AN N TPLH A ++ +LLL ADP + K+G+TPL + K+ +QD+ +LL A A + + +Q L ++N + G S +HL + + + +A L+ GAD+ PLH A+ +G +MA L+ +N + G+TPLH AAQ+ QL +LLL AD +KN +G
Sbjct: 10 ESNHSFLRAARAGNLSKVIDFLETGTDINTCNDIGLSA--LHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVALQQGHERIVALLLENDNRSKQRLPALHIAVKKNDVNAVNLLLNSSEVDVNHQSSSG--FTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNITPLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKLIDNGAD----------------------VMSKTRNGLTSLHMAAQGDFEDTARILLLKRANINETTTDFL---------------TPLHVTAHYGN-----EKVARVLLDRRCDPNARALN--GFTPLHIACKKHKIKIVELLLKYGALIEATTE--TGLTPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLAT------------------------------------RNNQTET---------------VKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPN---VATRD----NYTALHVAAKEGRSEAISLLLDSGAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLASQEGHTDVVSLLLQHKADVNHKAKNGLTS--MHLTAQEDKVTVARILVHAGADITPTTTAEYTPLHTAAHFGQINMAKYLISLMDQNQINQKTQLGFTPLHLAAQQGHLQLVNLLLESRADPNIKNNQG 769 HSP 2 Score: 178.333 bits (451), Expect = 1.511e-44 Identity = 202/712 (28.37%), Postives = 306/712 (42.98%), Query Frame = 2
Query: 680 LLEAARNG-LEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNE-EINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGY---RASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFE--GEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-------------VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
L AAR G L + I + T ++N G + LHLAA IV+ L++ ++ A K G LH A G EV K L+E GA VN +TPL+ AA + EV + L+ GAD T +PL +A L H+ V +L N N S KQ+L +H + N+V N + LL +N + G +PLH+A N QLL+ + ++N +T LH+AA+ S + +L++ G+ + T G S + LCE L +D A +S + N T + + FE I + + E TT ++ + + G+ ++ +L+ D N R +G TPLH A ++I +V++LL+ GA + A + GL PLH + G + +L+Q AN+ + T LH A + E KLLL+ A P + ++ TPL + ++ G + +AL AAK+G+ + L+ S R TPLH+A+ Y+ + +A LL++ A+ NA K GL PLH A+ Y H +A LLL A+ K GYTPLH AA+KN L +LL AD+ ++ G SPL L S
Sbjct: 15 FLRAARAGNLSKVIDFLETGTDINTCNDIGLSA--LHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVA--------LQQGHERIVAL----------------LLENDNRS-------KQRL----------PALHIAVKKNDV-----NAVNLLLNSSEVD-VNHQSSSGFTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNIT---PLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKL-----IDNGADVMSKTR------------NGLTSLHMAAQGDFEDTARILLLKRANINETTT------DFLTPLHVTAHYGNEKVARVLLDRRCD-----PNARALNG--FTPLHIACKKHKIKIVELLLKYGALIEATTETGLTPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLATRNNQTETVKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPNVATRDNYTALHVAAKEGRSEAISLLLDS-GAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLAS 643 HSP 3 Score: 139.428 bits (350), Expect = 1.213e-32 Identity = 103/318 (32.39%), Postives = 154/318 (48.43%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYX--------XXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
L A++AG++ V LE +DI + C D + LH AA I +VK L++ + A K G LH A G +V ++L++ ANVN+ FTPL+ AA + E+ L+ AD +DG TPL + +++G + + AL A KK + V L+ S ++ TPLH+A+ Y N + A+ L+EQ ADVN Q K + PLH A+ +G + M LL+ A V+ + G TPLH +A+ QLC L+ +GAD K G + L + + D R LL
Sbjct: 15 FLRAARAGNLSKVIDFLETGTDINT----CNDIG---LSALHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVALQQGHERIVALLLENDNRSKQRLPALHIAVKKNDVNAVNLLLNSSEVDVNHQSSSGFTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNITPLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKLIDNGADVMSKTRNGLTSLHMAAQGDFEDTARILL 325 HSP 4 Score: 128.257 bits (321), Expect = 2.497e-29 Identity = 123/434 (28.34%), Postives = 190/434 (43.78%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQEL------LEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAAT-DELRELLYFEHKG-----------FVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLL 1456
ACK + +++V+ LL G + + G TPLH ++ G KE V L+ +GAN+ G LH A + VK+L+ AG+ P+AK N TPLH A G ++ + LQ+ +D N+ D TA +A L+ +G + E L A + + +IL N +A+ TPLH+A YN ++ QLLL+ A K K G PLH A + ++A LL A NA ++PL A+ +G T+V SLLL + AD H + + + L A D++ H G L G+ N K ++ ++ D N N K QL G T +H ++ L+ LL A N+ N QG + H+A +++ ++L
Sbjct: 378 ACKKHKIKIVELLLKYGALIEATTETGL--TPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLATRNNQTETVKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPNVATRDNYTALHVAAKEGRSEAISLLLDSGAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLASQEGHTDVVSLLLQHKADVNHKAKNGLTSMHLTAQEDKVTVARILVHAGADITPTTTAEYTPLHTAAHFGQINMAKYLISLM----DQNQIN--QKTQL--------GFTPLHLAAQQGHL-----QLVNLLLESRADP--NIKNNQGWTAAHIARKQHYLNIFEVL 788
BLAST of Poly [ADP-ribose] polymerase vs. Planmine SMEST
Match: SMESG000000606.1 (SMESG000000606.1) HSP 1 Score: 231.106 bits (588), Expect = 9.670e-61 Identity = 223/870 (25.63%), Postives = 368/870 (42.30%), Query Frame = 2
Query: 200 ENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNN----TKLVFTGEYKKQELLEAARNGLEETIMTIL------TPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQK-------------LVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKY--SANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEG 2731
E+ + A + +L V L+ G D+N+ ++ G + LH A+ G +V+ LI R + + G LH A G +VVK+L+ G++ N + +TPL+ AA + +V L+ AD + DG T +A L+ + + ++ L A +++ + + + +VN +S G TPLH+AA Y + QLL+E A V+ + K + PLH A +G + + L++ GA+V+ TPLH +A G ++C L+ GAD ++ G + G++ R+L + + ++F+ T +H T N + + LL R + LN G +PLH+A + +++LL++ + E TG LH++A + S +L+ G+N T +G A L T NN+T+ V+ +K D + E + A+K G++EL+ L++ SD V RD + T LH AA R + +LL++GA + A+ K G PLH A Y VA +L+Q AN N TPLH A ++ +LLL ADP + K+G+TPL + K+ +QD+ +LL A A + + +Q L ++N + G S +HL + + + +A L+ GAD+ PLH A+ +G +MA L+ +N + G+TPLH AAQ+ QL +LLL AD +KN +G
Sbjct: 10 ESNHSFLRAARAGNLSKVIDFLETGTDINTCNDIGLSA--LHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVALQQGHERIVALLLENDNRSKQRLPALHIAVKKNDVNAVNLLLNSSEVDVNHQSSSG--FTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNITPLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKLIDNGAD----------------------VMSKTRNGLTSLHMAAQGDFEDTARILLLKRANINETTTDFL---------------TPLHVTAHYGN-----EKVARVLLDRRCDPNARALN--GFTPLHIACKKHKIKIVELLLKYGALIEATTE--TGLTPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLAT------------------------------------RNNQTET---------------VKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPN---VATRD----NYTALHVAAKEGRSEAISLLLDSGAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLASQEGHTDVVSLLLQHKADVNHKAKNGLTS--MHLTAQEDKVTVARILVHAGADITPTTTAEYTPLHTAAHFGQINMAKYLISLMDQNQINQKTQLGFTPLHLAAQQGHLQLVNLLLESRADPNIKNNQG 769 HSP 2 Score: 178.333 bits (451), Expect = 1.617e-44 Identity = 202/712 (28.37%), Postives = 306/712 (42.98%), Query Frame = 2
Query: 680 LLEAARNG-LEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNE-EINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGY---RASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFE--GEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-------------VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
L AAR G L + I + T ++N G + LHLAA IV+ L++ ++ A K G LH A G EV K L+E GA VN +TPL+ AA + EV + L+ GAD T +PL +A L H+ V +L N N S KQ+L +H + N+V N + LL +N + G +PLH+A N QLL+ + ++N +T LH+AA+ S + +L++ G+ + T G S + LCE L +D A +S + N T + + FE I + + E TT ++ + + G+ ++ +L+ D N R +G TPLH A ++I +V++LL+ GA + A + GL PLH + G + +L+Q AN+ + T LH A + E KLLL+ A P + ++ TPL + ++ G + +AL AAK+G+ + L+ S R TPLH+A+ Y+ + +A LL++ A+ NA K GL PLH A+ Y H +A LLL A+ K GYTPLH AA+KN L +LL AD+ ++ G SPL L S
Sbjct: 15 FLRAARAGNLSKVIDFLETGTDINTCNDIGLSA--LHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVA--------LQQGHERIVAL----------------LLENDNRS-------KQRL----------PALHIAVKKNDV-----NAVNLLLNSSEVD-VNHQSSSGFTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNIT---PLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKL-----IDNGADVMSKTR------------NGLTSLHMAAQGDFEDTARILLLKRANINETTT------DFLTPLHVTAHYGNEKVARVLLDRRCD-----PNARALNG--FTPLHIACKKHKIKIVELLLKYGALIEATTETGLTPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLATRNNQTETVKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPNVATRDNYTALHVAAKEGRSEAISLLLDS-GAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLAS 643 HSP 3 Score: 139.428 bits (350), Expect = 1.234e-32 Identity = 103/318 (32.39%), Postives = 154/318 (48.43%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYX--------XXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
L A++AG++ V LE +DI + C D + LH AA I +VK L++ + A K G LH A G +V ++L++ ANVN+ FTPL+ AA + E+ L+ AD +DG TPL + +++G + + AL A KK + V L+ S ++ TPLH+A+ Y N + A+ L+EQ ADVN Q K + PLH A+ +G + M LL+ A V+ + G TPLH +A+ QLC L+ +GAD K G + L + + D R LL
Sbjct: 15 FLRAARAGNLSKVIDFLETGTDINT----CNDIG---LSALHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVALQQGHERIVALLLENDNRSKQRLPALHIAVKKNDVNAVNLLLNSSEVDVNHQSSSGFTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNITPLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKLIDNGADVMSKTRNGLTSLHMAAQGDFEDTARILL 325 HSP 4 Score: 128.257 bits (321), Expect = 2.605e-29 Identity = 123/434 (28.34%), Postives = 190/434 (43.78%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQEL------LEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAAT-DELRELLYFEHKG-----------FVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLL 1456
ACK + +++V+ LL G + + G TPLH ++ G KE V L+ +GAN+ G LH A + VK+L+ AG+ P+AK N TPLH A G ++ + LQ+ +D N+ D TA +A L+ +G + E L A + + +IL N +A+ TPLH+A YN ++ QLLL+ A K K G PLH A + ++A LL A NA ++PL A+ +G T+V SLLL + AD H + + + L A D++ H G L G+ N K ++ ++ D N N K QL G T +H ++ L+ LL A N+ N QG + H+A +++ ++L
Sbjct: 378 ACKKHKIKIVELLLKYGALIEATTETGL--TPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLATRNNQTETVKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPNVATRDNYTALHVAAKEGRSEAISLLLDSGAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLASQEGHTDVVSLLLQHKADVNHKAKNGLTSMHLTAQEDKVTVARILVHAGADITPTTTAEYTPLHTAAHFGQINMAKYLISLM----DQNQIN--QKTQL--------GFTPLHLAAQQGHL-----QLVNLLLESRADP--NIKNNQGWTAAHIARKQHYLNIFEVL 788
BLAST of Poly [ADP-ribose] polymerase vs. Planmine SMEST
Match: SMESG000000606.1 (SMESG000000606.1) HSP 1 Score: 231.106 bits (588), Expect = 9.712e-61 Identity = 223/870 (25.63%), Postives = 368/870 (42.30%), Query Frame = 2
Query: 200 ENCRTLFDACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNN----TKLVFTGEYKKQELLEAARNGLEETIMTIL------TPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNEEINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGYRASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFEGEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDLV-KEGDQDVYXXXXXXXXXXXXXKKGQIAKVQK-------------LVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKY--SANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEG 2731
E+ + A + +L V L+ G D+N+ ++ G + LH A+ G +V+ LI R + + G LH A G +VVK+L+ G++ N + +TPL+ AA + +V L+ AD + DG T +A L+ + + ++ L A +++ + + + +VN +S G TPLH+AA Y + QLL+E A V+ + K + PLH A +G + + L++ GA+V+ TPLH +A G ++C L+ GAD ++ G + G++ R+L + + ++F+ T +H T N + + LL R + LN G +PLH+A + +++LL++ + E TG LH++A + S +L+ G+N T +G A L T NN+T+ V+ +K D + E + A+K G++EL+ L++ SD V RD + T LH AA R + +LL++GA + A+ K G PLH A Y VA +L+Q AN N TPLH A ++ +LLL ADP + K+G+TPL + K+ +QD+ +LL A A + + +Q L ++N + G S +HL + + + +A L+ GAD+ PLH A+ +G +MA L+ +N + G+TPLH AAQ+ QL +LLL AD +KN +G
Sbjct: 10 ESNHSFLRAARAGNLSKVIDFLETGTDINTCNDIGLSA--LHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVALQQGHERIVALLLENDNRSKQRLPALHIAVKKNDVNAVNLLLNSSEVDVNHQSSSG--FTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNITPLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKLIDNGAD----------------------VMSKTRNGLTSLHMAAQGDFEDTARILLLKRANINETTTDFL---------------TPLHVTAHYGN-----EKVARVLLDRRCDPNARALN--GFTPLHIACKKHKIKIVELLLKYGALIEATTE--TGLTPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLAT------------------------------------RNNQTET---------------VKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPN---VATRD----NYTALHVAAKEGRSEAISLLLDSGAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLASQEGHTDVVSLLLQHKADVNHKAKNGLTS--MHLTAQEDKVTVARILVHAGADITPTTTAEYTPLHTAAHFGQINMAKYLISLMDQNQINQKTQLGFTPLHLAAQQGHLQLVNLLLESRADPNIKNNQG 769 HSP 2 Score: 178.333 bits (451), Expect = 1.412e-44 Identity = 202/712 (28.37%), Postives = 306/712 (42.98%), Query Frame = 2
Query: 680 LLEAARNG-LEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAATDELRELLYFEHKGFVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLLVRNE-EINIDMLEGVTGKNALHLAAELNSSSICYILVNYGSNKTIPTNQGY---RASQLTTDIDLCEFLSEKEYLDPTAACISDSNKIVPTFKSKLFTNNKTDKWNSKEPKFE--GEIAPPVESTMKEKTTNDKQVEEYCMQILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-------------VKEGDQDVYXXXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCS 2755
L AAR G L + I + T ++N G + LHLAA IV+ L++ ++ A K G LH A G EV K L+E GA VN +TPL+ AA + EV + L+ GAD T +PL +A L H+ V +L N N S KQ+L +H + N+V N + LL +N + G +PLH+A N QLL+ + ++N +T LH+AA+ S + +L++ G+ + T G S + LCE L +D A +S + N T + + FE I + + E TT ++ + + G+ ++ +L+ D N R +G TPLH A ++I +V++LL+ GA + A + GL PLH + G + +L+Q AN+ + T LH A + E KLLL+ A P + ++ TPL + ++ G + +AL AAK+G+ + L+ S R TPLH+A+ Y+ + +A LL++ A+ NA K GL PLH A+ Y H +A LLL A+ K GYTPLH AA+KN L +LL AD+ ++ G SPL L S
Sbjct: 15 FLRAARAGNLSKVIDFLETGTDINTCNDIGLSA--LHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVA--------LQQGHERIVAL----------------LLENDNRS-------KQRL----------PALHIAVKKNDV-----NAVNLLLNSSEVD-VNHQSSSGFTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNIT---PLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKL-----IDNGADVMSKTR------------NGLTSLHMAAQGDFEDTARILLLKRANINETTT------DFLTPLHVTAHYGNEKVARVLLDRRCD-----PNARALNG--FTPLHIACKKHKIKIVELLLKYGALIEATTETGLTPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLATRNNQTETVKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPNVATRDNYTALHVAAKEGRSEAISLLLDS-GAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLAS 643 HSP 3 Score: 139.428 bits (350), Expect = 1.268e-32 Identity = 103/318 (32.39%), Postives = 154/318 (48.43%), Query Frame = 2
Query: 1865 ILEASKAGDVELVCRILENNSDIKSVLVNCRDTDGRHSTPLHFAAGFNRISVVKVLLENGADVHAKDKGGLVPLHNACSYGHAKVAELLIQYNANVNVCDLWKFTPLHEAAAKGKFEICKLLLQYNADPHKRNKDGHTPLDL-VKEGDQDVYX--------XXXXXXXXXXXXKKGQIAKVQKLVTSVNINCRDTQGRNSTPLHLASGYNNLEIAEFLLEQGADVNAQDKGGLIPLHNASSYGHADMAALLLKYSANVNSADKWGYTPLHEAAQKNRTQLCSLLLAHGADAKLKNFEGQSPLDLCSAAD----VRFLL 2779
L A++AG++ V LE +DI + C D + LH AA I +VK L++ + A K G LH A G +V ++L++ ANVN+ FTPL+ AA + E+ L+ AD +DG TPL + +++G + + AL A KK + V L+ S ++ TPLH+A+ Y N + A+ L+EQ ADVN Q K + PLH A+ +G + M LL+ A V+ + G TPLH +A+ QLC L+ +GAD K G + L + + D R LL
Sbjct: 15 FLRAARAGNLSKVIDFLETGTDINT----CNDIG---LSALHLAAKEGHIVIVKELIKREPRIDATTKKGNSALHIASLAGKFEVVKILVENGANVNLQSTNGFTPLYMAAQENHLEVVTFLINNGADQTITTEDGFTPLAVALQQGHERIVALLLENDNRSKQRLPALHIAVKKNDVNAVNLLLNSSEVDVNHQSSSGFTPLHIAAHYGNEKSAQLLIEQKADVNFQAKNNITPLHVAAKWGRSGMVQLLIDSGALVSCRTRDGLTPLHCSARSGHAQLCEKLIDNGADVMSKTRNGLTSLHMAAQGDFEDTARILL 325 HSP 4 Score: 128.257 bits (321), Expect = 2.547e-29 Identity = 123/434 (28.34%), Postives = 190/434 (43.78%), Query Frame = 2
Query: 224 ACKNNDLQVVKCLLDIGYDVNSKDNFGRKSTPLHFASGFGRKEMVEFLISRGANVSIKDEGGLIPLHNACSFGHADVVKVLISAGSDPNAKDVWNYTPLHEAAVKGKADVCIVLLQAKADVNLKNSDGLTAFDLATDNNTK-----LVFTGEYKKQEL------LEAARNGLEETIMTILTPFNVNCHASDGRKSTPLHLAAGYNRCRIVQLLLEMGAQVHAKDKGGLVPLHNACSYGHIEVAKSLLERGAMVNAPDLWQYTPLHEAASKGRTEVCSLLLAYGADPTHLNCHNKSPLELAAT-DELRELLYFEHKG-----------FVLFNYCRLGEYNKVKRMLQMLGNTNDSNCSNFIDKQQLINFVHPFTGDTLIHATISSNNVISKRKNLIEFLLRRGATSSLNVLNKQGSSPLHLAILTKNVDLIQLL 1456
ACK + +++V+ LL G + + G TPLH ++ G KE V L+ +GAN+ G LH A + VK+L+ AG+ P+AK N TPLH A G ++ + LQ+ +D N+ D TA +A L+ +G + E L A + + +IL N +A+ TPLH+A YN ++ QLLL+ A K K G PLH A + ++A LL A NA ++PL A+ +G T+V SLLL + AD H + + + L A D++ H G L G+ N K ++ ++ D N N K QL G T +H ++ L+ LL A N+ N QG + H+A +++ ++L
Sbjct: 378 ACKKHKIKIVELLLKYGALIEATTETGL--TPLHVSAFIGSKEAVNMLLQKGANIEQGTMRGETALHLATRNNQTETVKLLLKAGAIPDAKARENQTPLHVATKIGNLELITLFLQSGSDPNVATRDNYTALHVAAKEGRSEAISLLLDSGAKIEAETKRGFTPLHIAAKYDKVGVASILLQKKANPNATGKNGLTPLHVATHYNHEKVAQLLLDNKADPKCKAKNGYTPLHIAAKKNNQDLAIMLLVSDADSNAESRNGFSPLQLASQEGHTDVVSLLLQHKADVNHKAKNGLTSMHLTAQEDKVTVARILVHAGADITPTTTAEYTPLHTAAHFGQINMAKYLISLM----DQNQIN--QKTQL--------GFTPLHLAAQQGHL-----QLVNLLLESRADP--NIKNNQGWTAAHIARKQHYLNIFEVL 788 The following BLAST results are available for this feature:
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Poly [ADP-ribose] polymerase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010139 ID=SMED30010139|Name=Poly [ADP-ribose] polymerase|organism=Schmidtea mediterranea sexual|type=transcript|length=4145bpback to top protein sequence of SMED30010139-orf-1 >SMED30010139-orf-1 ID=SMED30010139-orf-1|Name=SMED30010139-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1343bp MNHEIYRNRSVSEGEVNCASYNPNEYCTDIQDICDKIADTSLSKLTEASFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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