SMED30010117

Overview
NameSMED30010117
Smed IDSMED30010117
Length (bp)6544
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010117 (SMED30010117) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010117 (SMED30010117) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010117 (SMED30010117) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010117 (SMED30010117) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010117

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neoblastSMED30010117SMESG000030021.1 SMESG000000893.1 dd_Smed_v4_2033_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30010117SMESG000030021.1 SMESG000000893.1 dd_Smed_v4_2033_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30010117SMESG000000893.1 dd_Smed_v4_5234_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010117SMESG000000893.1 dd_Smed_v4_5234_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010117SMESG000030021.1 SMESG000000893.1 dd_Smed_v4_2033_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
non-ciliated epidermisSMED30010117SMESG000030021.1 SMESG000000893.1 dd_Smed_v4_2033_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010117SMESG000000893.1 dd_Smed_v4_5234_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30010117SMESG000000893.1 dd_Smed_v4_5234_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30010117SMESG000000893.1 dd_Smed_v4_5234_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30010117 vs. Planmine SMEST
Match: SMESG000000893.1 (SMESG000000893.1)

HSP 1 Score: 4060.76 bits (10530), Expect = 0.000e+0
Identity = 2105/2128 (98.92%), Postives = 2111/2128 (99.20%), Query Frame = 1 
Query:   46 IMNSFSYCSQISFNDCVSYELDSWTKLVKFDESQNNSKIDQSILNKHIYNDGVSKIFRQISRSKLIICCIKRQNVKIKQLIITEFKAPXXXXXXXXXXXXXXXXYTRKEMNTILIKIEKTDSFFLSEIRNEISSLEESCEFCVEFPINENEITNEFCLSADVINCILNDCGLDLEELDRIVSTEIEDFENKLDPLKRSNLSTIQKSRIERRFNKVLSVFEKDVCSNLTFISKILTRLFFGYSNLIIDPHYFDLTPDELEKYYCELNQFQLQNIINMKSFLLDLEKKVTEYILFMKEVQMILSSGNVTHQEKNSLNFLENILSPIRTSSTKRNLNYELASSFLSSTPGPRRLTKDMKXXXXXXXXXXXXXXXXXXXFDSGYLLTSGNEHSFRDSYDNFDVIPFSDDEYEKILDDDLDIPETEEDLEFCDSICTTSEIGIPCSEINSEFSEINFSVSSLVADLDFDSKAFCRKNNFCKINSVGKFLFSSSGNSEFYEKNFIDIPIVLFKRMNMMNSLVFDIELVNDLERIDRDIRQKIALLINSDEWESKYNNVLHITLQRIRQFPLIDMSDQFEYLTTIIKSCLSTKINKMTDFETTTNVGVQFNNSAYTTYTFCDNNIVSCEKNTNNNADSKDDDSIDLSATEHSCSINKNDQSDSLCLDAELQVAFKTILSDSISNEHIVDTSELSNVNQLNEVAKFNNYRDVTVNIYNTSLSDESSNEWPDHNSSDLECASQDEAKSKQTSEYIEDVKLVVKLSELHLRSEYQSEQHNKSTLVYDKNLIVDKYKSLKSHLIYSSDDDNNKTGDWNGNCTKSPTENRNKTNVCVQIRKSVHEPYQIENSEIIDNCDTDRDKSEETETEFYNNINTKIQSDLYQNTEDPLQSDQPLLKELGIVNDNQSLSYTDRISLTIDLTEKLLDLTNYRLKFISSLFNEKISSAKSTMLRNKSLTICALKQRLLITLKILSNLKFAIKNTYDSEIQDPMYSKIETLISKSETLTKFAVDIENQMKTNGCDMLKNDCYNFENELPDDLLIKIEPNDLDVIVSISTCRLQITIASLSQYPCYNYFQKSQLPKISKLINISLPVNCNLEDTLNPSDLLQKLCKLRRITAICALNIVDLHVILTKLEHDGSSAHNSIENKVKNLLIVWRAVDGECHRLLRCTSIVYHIMTDTRKVYIDLISLLAVTQSIIDTVELSKTEMDTLKTNIESLMNYKQRILEWLEEIHLCVSVIYKKRMTNKRFKYYDEIHHFLEENLNTITELTDRIKNGCNQAANIVNDVITYCSNLPHCVITDIGKVLITDSWINNKHLYYSECKTEEVNENIFHAIIDLEELTRRINNCIITKENLKSFSDQFQEFLDQSKDIFQVINVENMDVIVSEKNNSSMIPRQIFRDSKDQSQLIFSDESVTDNAAGKIMDKISKIAACAAENALILFIDKYELEFSDNNDTDHQISLLTSFSDSSDIMKEFCYRIVYNVGASLTLSIIDNILSVTQGHLLVESGMDLKGICQDLSYRTVSTIVQQLLSDQEVMNEMFLSETSNDIANYLCEELGCLMVNNSLQEIQVVDAPTNRNIIFISTCVSNLENKPLFGDLVEKSRFISSDVIGSQFEQMNSMRLSISDTQDPNYQENLVKEFSILPENSFNAVPIGILKQKYDSQEICKSFTRFLDPKTAVVEYHVRQLASKLNYYKCVTNQCKINKPPIKEINFPKQKFNRITKLVAKFNRNTAAEKSQLTIVPLIKQPSLERIFLNLLNVKSLQMVEEIARDALKQHQVYSVYPMVIENCENESPIHSLSNSRNSVVQRLEYNGSPGEVKNQVDRSEIVLHRDCGRMRSIKHSNTFNYEFDNSINANYKSQCLPIICRTPLKETESEIQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGSETIFIGNSETIIKEIKSNSEADFHENNSPKEIDSTEDYFSMKIPMKFQAVENRNNNLEIIFREKINFNASEIENQLFHFPKQFVSPVESISTKHFPSNCCRVSERESLCKIDSIRKSSKSADISMENKTINFRNNQNLTELSLLSSQTANSTGFVVADSSHIQLSKREKNSFFSKFWXXXXXXXX--XXXXYKGQLSQICNCLAFGQLLYPKISSSGHPV 6423
            IMNSFSYCSQISFNDCVSYELDSWTKLVKFDESQNNSKIDQSILNKHIYNDGVSKIFRQISRSKLIICCIKRQNVKIKQLIITEFKAPLLFEKIKLIDLIKLLIYTRKEMNTILIKIEKTDSFFLSEIRNEISSLEESCEFCVEFPINENEITNEFCLSADVINCILNDCGLDLEELDRIVSTEIEDFENKLDPLKRSNLSTIQKSRIERRFNKVLSVFEKDVCSNLTFI+KILTRLFFGYSNLIIDPHYFDLTPDELEKYYCELNQFQLQ IINMKSFLLDLEKKVTEYILFMKEVQMILSSGNVTHQEKNSLNFLENILSPIRTSSTKRNLNYELASSFLSSTPGPRRLTKDMKSYSILSESNEIINEKSISSFDSGYLLTSGNEHSFRDSYDNFDVIPFSDDEYEKILDDDLDIPETEEDLEFCDSICTTSEIGIPCSEINSEFSEINFSVSSLVADLDFDSKAFCRKNNFCKINSVGKFLFSSSGNS+FYE NFIDIPIVLFKRMNMMNSLVFDIELVNDLERIDRDIRQKIALLINSDEWESKYNNVLHITLQRIRQFPLIDMSDQFEYLTTIIKSCLSTKINKMTDFETTTNVGVQFNNSAYTTYTFCDNNIVSCEKNTNNNADSKDDDSIDLSATEHSCSINKNDQSDSLCLDAELQVAFKTILSDSISNEHIVDTSELSNVNQLNEVAKFNNYRDVTVNIYNTSLSDESS EWPDHNSSDLECASQDEAKSKQTSEYIEDVKLVVKLSELHLRSEYQSEQHNKSTLVYDKNLIVDKYKSLKSHLIYSSDDDNNKTGDWNGNCTKSPTENRNKTNVCVQIR SVHEPYQIENSEIIDNCDTDRDKSEETETEFYNNINTKIQSDLYQNTEDPLQSDQPLLK       + SLSYTDRISLTIDLTEKLLDLTNYRLKFISSLFNEKISSAKSTMLRNKSLTICALKQRLLITLKILSNLKFAIKNTYDSEIQDPM+SKIETLISKSETLTKFAVDIENQMKTNGCDMLKNDCYNFENELPDDLLIKIEPNDLDVIVSISTCRLQITIASLSQYPCYNYFQKSQLPKISKLINISLPVNCNLE+TLNPSDLLQKLCKLRRITAICALNIVDLHVILTKLEHDGSSAHNSIENKVKNLLIVWRAVDGECHRLLRCTSIVYHIMTDTRKVYIDLISLLAVTQSIIDTVELSKTEMDTLKTNIESLMNYKQRILEWLEEIHLCVS+IYKKRMTNKRFKYYDEIHHFLEENLNTITELTDRIKNGCNQAANIVNDVITYCSNLPHCVITDIGKVLITDSWINNKHLYYSECKTEEVNENIFHAIIDLEELTRRINNCIITKENLKSFSDQFQEFLDQSKDIFQVINVENMDVIVSEKNNSSMIPRQIFRDSKDQSQLIFSDESVTDNAAGKIMDKISKIAACAAENALILFIDKYELEFSDNNDTDHQISLLTSFSDSSDIMKEFCYRIVYNVGASLTLSIIDNILSVTQGHLLVESGMDLKGICQDLSYRTVSTIVQQLLSDQEVMNEMFLSETSNDIANYLCEELGCLMVNNSLQEIQVVDAPTNRNIIFISTCVSNLENKPLFGDLVEKSRFISSDVIGSQFEQMNSMRLSISDTQDPNYQENLVKEFSILPENSFNAVPIGILKQKYDSQEICKSFTRFLDPKTAVVEYHVRQLASKLNYYKCVTNQCKINKPPIKEINFPKQKFNRITKLVAKFNRNTAAEKSQLTIVPLIKQPSLERIFLNLLNVKSLQMVEEIARDALKQHQVYSVYPMVIENCENESPIHSLSNSRNSVVQRLEYNGSPGEVKNQVDRSEIVLHRDCGRMRSIKHSNTFNYEFDNSINANYKSQCLPIICRTPLKETESEIQSPLLELTVDELLLLSSSSSSSSFNSVNNDNNRLTGSETIFIGNSETIIKEIKSNSEADFHENNSPKEIDSTEDYFSMKIPMKFQAVENRNNNLEIIFREKIN N SEIENQLFHFPKQFVSPVESISTKHFPSNCCRVSERESLCKIDSIRKSSKSADISMENKTINFRNNQNLTELSLLSSQTANSTGFVVADSSHIQLSKREKNSFFSKFWLLIVALI   VLVLYKGQLSQICNCLAFGQLLYPKISSSGHPV
Sbjct:    9 IMNSFSYCSQISFNDCVSYELDSWTKLVKFDESQNNSKIDQSILNKHIYNDGVSKIFRQISRSKLIICCIKRQNVKIKQLIITEFKAPLLFEKIKLIDLIKLLIYTRKEMNTILIKIEKTDSFFLSEIRNEISSLEESCEFCVEFPINENEITNEFCLSADVINCILNDCGLDLEELDRIVSTEIEDFENKLDPLKRSNLSTIQKSRIERRFNKVLSVFEKDVCSNLTFINKILTRLFFGYSNLIIDPHYFDLTPDELEKYYCELNQFQLQKIINMKSFLLDLEKKVTEYILFMKEVQMILSSGNVTHQEKNSLNFLENILSPIRTSSTKRNLNYELASSFLSSTPGPRRLTKDMKSYSILSESNEIINEKSISSFDSGYLLTSGNEHSFRDSYDNFDVIPFSDDEYEKILDDDLDIPETEEDLEFCDSICTTSEIGIPCSEINSEFSEINFSVSSLVADLDFDSKAFCRKNNFCKINSVGKFLFSSSGNSKFYENNFIDIPIVLFKRMNMMNSLVFDIELVNDLERIDRDIRQKIALLINSDEWESKYNNVLHITLQRIRQFPLIDMSDQFEYLTTIIKSCLSTKINKMTDFETTTNVGVQFNNSAYTTYTFCDNNIVSCEKNTNNNADSKDDDSIDLSATEHSCSINKNDQSDSLCLDAELQVAFKTILSDSISNEHIVDTSELSNVNQLNEVAKFNNYRDVTVNIYNTSLSDESSTEWPDHNSSDLECASQDEAKSKQTSEYIEDVKLVVKLSELHLRSEYQSEQHNKSTLVYDKNLIVDKYKSLKSHLIYSSDDDNNKTGDWNGNCTKSPTENRNKTNVCVQIRNSVHEPYQIENSEIIDNCDTDRDKSEETETEFYNNINTKIQSDLYQNTEDPLQSDQPLLKNWLTCLYSNSLSYTDRISLTIDLTEKLLDLTNYRLKFISSLFNEKISSAKSTMLRNKSLTICALKQRLLITLKILSNLKFAIKNTYDSEIQDPMFSKIETLISKSETLTKFAVDIENQMKTNGCDMLKNDCYNFENELPDDLLIKIEPNDLDVIVSISTCRLQITIASLSQYPCYNYFQKSQLPKISKLINISLPVNCNLENTLNPSDLLQKLCKLRRITAICALNIVDLHVILTKLEHDGSSAHNSIENKVKNLLIVWRAVDGECHRLLRCTSIVYHIMTDTRKVYIDLISLLAVTQSIIDTVELSKTEMDTLKTNIESLMNYKQRILEWLEEIHLCVSIIYKKRMTNKRFKYYDEIHHFLEENLNTITELTDRIKNGCNQAANIVNDVITYCSNLPHCVITDIGKVLITDSWINNKHLYYSECKTEEVNENIFHAIIDLEELTRRINNCIITKENLKSFSDQFQEFLDQSKDIFQVINVENMDVIVSEKNNSSMIPRQIFRDSKDQSQLIFSDESVTDNAAGKIMDKISKIAACAAENALILFIDKYELEFSDNNDTDHQISLLTSFSDSSDIMKEFCYRIVYNVGASLTLSIIDNILSVTQGHLLVESGMDLKGICQDLSYRTVSTIVQQLLSDQEVMNEMFLSETSNDIANYLCEELGCLMVNNSLQEIQVVDAPTNRNIIFISTCVSNLENKPLFGDLVEKSRFISSDVIGSQFEQMNSMRLSISDTQDPNYQENLVKEFSILPENSFNAVPIGILKQKYDSQEICKSFTRFLDPKTAVVEYHVRQLASKLNYYKCVTNQCKINKPPIKEINFPKQKFNRITKLVAKFNRNTAAEKSQLTIVPLIKQPSLERIFLNLLNVKSLQMVEEIARDALKQHQVYSVYPMVIENCENESPIHSLSNSRNSVVQRLEYNGSPGEVKNQVDRSEIVLHRDCGRMRSIKHSNTFNYEFDNSINANYKSQCLPIICRTPLKETESEIQSPLLELTVDELLLLSSSSSSSSFNSVNNDNNRLTGSETIFIGNSETIIKEIKSNSEADFHENNSPKEIDSTEDYFSMKIPMKFQAVENRNNNLEIIFREKINSNVSEIENQLFHFPKQFVSPVESISTKHFPSNCCRVSERESLCKIDSIRKSSKSADISMENKTINFRNNQNLTELSLLSSQTANSTGFVVADSSHIQLSKREKNSFFSKFWLLIVALILIFVLVLYKGQLSQICNCLAFGQLLYPKISSSGHPV 2136
The following BLAST results are available for this feature:
BLAST of SMED30010117 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010117 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000000893.10.000e+098.92SMESG000000893.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010117 ID=SMED30010117|Name=SMED30010117|organism=Schmidtea mediterranea sexual|type=transcript|length=6544bp
ATCTATATAAATCTTATATGATTAGAATGAAACATCCACAAGTACATAAT
GAACAGTTTTAGCTATTGCTCACAAATTTCATTTAATGATTGCGTTTCTT
ATGAATTAGATAGCTGGACAAAACTCGTGAAGTTTGATGAAAGTCAAAAT
AACTCCAAAATTGATCAATCAATACTAAATAAACATATTTATAATGATGG
AGTGAGTAAAATTTTTCGACAAATTAGTCGTTCGAAATTGATAATTTGTT
GTATTAAACGACAAAATGTCAAAATCAAACAATTGATTATCACAGAATTT
AAAGCTCCTCTTTTATTTGAAAAAATAAAACTCATTGATTTGATCAAATT
GCTGATCTATACTAGAAAAGAGATGAACACAATTTTAATTAAAATCGAAA
AAACCGATTCTTTCTTTTTGAGTGAAATAAGAAACGAAATTTCCAGTCTA
GAAGAATCATGTGAATTCTGTGTTGAATTTCCAATCAATGAAAACGAAAT
CACGAATGAATTTTGCCTGTCTGCCGATGTGATAAATTGCATACTGAATG
ATTGTGGATTAGATCTAGAAGAGCTAGATCGAATTGTTTCAACAGAAATC
GAAGATTTCGAAAATAAATTGGATCCACTGAAAAGGTCCAATCTGAGCAC
CATTCAAAAGTCGCGCATAGAACGTCGTTTTAACAAAGTTTTATCGGTTT
TTGAAAAAGACGTCTGTTCCAATTTGACTTTTATCAGCAAAATATTAACC
CGACTATTTTTCGGTTATTCTAATTTGATAATTGACCCCCATTACTTTGA
CTTAACTCCCGATGAACTGGAAAAATATTACTGTGAACTTAACCAGTTTC
AACTTCAAAATATTATTAATATGAAAAGCTTTTTGTTGGATCTTGAAAAA
AAAGTAACAGAATATATTTTGTTCATGAAAGAAGTGCAAATGATTTTAAG
CTCAGGAAATGTTACTCATCAAGAGAAAAATTCTTTGAATTTTCTAGAGA
ACATTTTGTCTCCAATCCGTACATCGTCAACTAAGAGAAATTTAAACTAT
GAATTGGCATCATCATTTCTGTCGTCAACTCCCGGACCTCGTCGGTTAAC
GAAAGATATGAAATCATATTCGATTCTTTCTGAATCGAATGAAATAATAA
ACGAAAAAAGCATTTCGTCTTTTGATTCGGGATATTTGTTGACTTCAGGA
AATGAGCATTCTTTCAGGGATAGTTATGATAATTTCGATGTTATCCCATT
CTCAGACGATGAATATGAGAAAATACTGGACGACGATTTAGATATTCCAG
AAACTGAGGAAGATCTGGAGTTTTGTGATTCAATATGTACGACATCTGAA
ATTGGGATTCCCTGCAGTGAAATTAATTCAGAATTTTCTGAAATTAATTT
TTCGGTTTCATCACTTGTAGCTGATTTAGATTTCGATTCAAAAGCATTTT
GTAGAAAAAATAATTTCTGTAAAATCAATTCAGTTGGGAAGTTTTTGTTT
TCGTCTAGCGGCAACTCAGAGTTTTATGAAAAAAATTTCATAGACATTCC
AATAGTGCTGTTTAAAAGAATGAATATGATGAATTCATTAGTATTTGACA
TTGAGCTGGTAAACGATCTGGAGAGGATAGATAGGGATATCCGACAGAAA
ATTGCATTATTAATAAACAGTGATGAATGGGAATCTAAATATAACAATGT
GTTGCACATAACTCTTCAAAGAATCCGTCAATTTCCTTTGATTGATATGA
GTGATCAGTTCGAATACCTAACAACAATAATAAAGTCATGCCTGTCAACA
AAAATAAACAAAATGACAGATTTCGAAACAACAACCAATGTTGGTGTTCA
GTTCAACAACAGCGCTTATACAACATACACTTTCTGTGATAACAATATTG
TCAGTTGTGAAAAAAACACAAATAATAATGCCGATTCTAAAGATGATGAT
TCCATTGATCTGAGCGCGACTGAGCACAGCTGCTCAATCAATAAAAACGA
TCAGAGTGATTCACTGTGTTTAGATGCAGAATTGCAAGTTGCATTTAAAA
CCATTCTATCAGATTCAATATCTAATGAACACATTGTCGACACCAGTGAA
CTGAGCAATGTGAATCAATTGAACGAGGTGGCCAAGTTTAACAATTACCG
AGATGTCACTGTCAACATTTATAATACATCATTGTCTGATGAATCAAGCA
ACGAGTGGCCTGATCATAATTCTAGTGATTTAGAATGTGCTTCTCAAGAC
GAAGCAAAGAGTAAACAGACGTCAGAATACATCGAGGACGTCAAATTAGT
TGTTAAACTTAGCGAGCTTCATTTGAGATCGGAATATCAATCGGAACAGC
ACAATAAATCGACTTTAGTTTATGATAAAAACCTAATAGTTGACAAGTAT
AAGAGTCTGAAATCGCATTTAATTTATAGTAGTGATGATGACAATAATAA
AACTGGCGATTGGAATGGAAACTGTACTAAAAGTCCTACGGAAAATCGCA
ACAAAACCAATGTTTGTGTTCAAATAAGGAAAAGTGTCCATGAGCCATAT
CAAATTGAGAACAGTGAAATAATAGATAATTGTGACACCGACAGAGATAA
AAGTGAAGAAACTGAAACAGAATTCTATAATAACATAAATACTAAAATCC
AATCTGATTTATATCAAAATACAGAGGACCCATTGCAATCTGATCAGCCA
TTGCTTAAAGAATTGGGGATTGTTAATGATAATCAGTCACTGAGTTACAC
TGATAGAATATCCTTAACAATTGATTTGACGGAGAAACTCCTCGATCTTA
CAAATTATCGATTGAAATTCATTTCATCTTTGTTCAATGAGAAAATTTCT
AGTGCCAAATCGACCATGTTACGAAATAAATCTTTAACCATATGTGCATT
GAAACAAAGATTATTAATAACTCTCAAAATACTCTCAAATTTAAAATTTG
CGATCAAAAATACTTATGACTCTGAAATTCAAGACCCAATGTACTCAAAA
ATAGAAACTTTAATCAGCAAAAGTGAAACGCTTACTAAATTTGCTGTTGA
TATTGAAAATCAAATGAAAACTAATGGATGTGATATGTTAAAAAATGATT
GTTATAATTTCGAAAACGAACTACCTGATGATCTTTTAATTAAAATCGAA
CCAAATGATCTTGATGTGATCGTGTCTATATCCACGTGCAGATTACAAAT
AACAATTGCGTCACTGTCACAATATCCGTGCTATAATTATTTCCAAAAAT
CACAGCTACCGAAGATTAGTAAATTAATAAATATTTCACTGCCAGTAAAT
TGTAATCTGGAAGATACTTTGAATCCTTCTGATCTTTTACAAAAATTATG
TAAATTAAGAAGAATAACGGCAATATGTGCTTTAAATATCGTAGATTTGC
ACGTGATTTTAACGAAATTAGAGCATGACGGATCATCAGCTCACAATTCA
ATAGAAAATAAAGTTAAAAATTTGTTGATAGTTTGGCGTGCAGTAGATGG
CGAATGCCATAGACTGTTGCGTTGTACTAGCATTGTCTATCACATCATGA
CCGATACTCGAAAAGTCTACATAGATCTAATATCACTTTTAGCTGTCACG
CAATCTATCATTGATACCGTTGAATTGTCCAAAACCGAAATGGATACTTT
GAAAACCAATATTGAATCTTTGATGAATTACAAACAACGAATTTTGGAAT
GGTTAGAAGAAATACACTTGTGTGTTTCGGTCATTTATAAAAAGAGAATG
ACAAATAAACGGTTTAAATATTATGACGAAATCCATCATTTTCTGGAAGA
AAATCTGAATACAATTACTGAATTGACTGATAGAATTAAAAATGGATGTA
ATCAGGCAGCCAATATTGTTAATGACGTAATCACTTATTGCTCAAACTTA
CCGCACTGCGTCATAACAGATATCGGGAAAGTATTGATCACAGATTCATG
GATCAATAATAAGCACTTGTATTATTCGGAATGTAAGACTGAGGAAGTCA
ATGAAAATATTTTCCACGCAATCATAGATTTAGAAGAATTGACAAGGAGA
ATTAATAATTGCATCATCACGAAAGAGAATCTAAAATCATTCTCAGATCA
ATTCCAGGAATTTCTTGATCAGAGTAAGGATATATTTCAGGTGATTAATG
TTGAAAACATGGACGTTATTGTTTCGGAGAAAAACAACTCCAGTATGATT
CCCCGACAAATTTTCCGAGATTCGAAGGATCAAAGCCAATTAATATTTTC
AGATGAAAGTGTCACAGATAATGCGGCGGGAAAAATAATGGATAAAATAT
CAAAAATCGCCGCTTGTGCAGCAGAAAATGCTCTAATATTATTTATAGAC
AAATATGAGTTAGAATTTTCTGATAACAATGATACCGATCATCAGATCAG
TTTACTTACAAGTTTCTCAGATAGCAGTGATATAATGAAAGAGTTTTGTT
ATCGAATTGTCTACAACGTCGGAGCATCTTTGACTTTGTCTATAATCGAC
AATATCCTAAGTGTTACACAGGGACATTTGTTAGTTGAATCTGGCATGGA
TTTAAAGGGTATTTGTCAAGATTTGAGTTATCGAACCGTTTCAACAATTG
TCCAGCAACTCCTTTCCGATCAAGAAGTTATGAATGAGATGTTTCTCTCT
GAAACAAGCAATGATATTGCCAATTATCTATGTGAAGAATTAGGTTGTTT
GATGGTTAACAATAGTCTGCAAGAAATACAAGTCGTTGATGCTCCCACAA
ACCGCAACATAATTTTCATTTCCACTTGTGTTTCAAATTTGGAAAATAAA
CCATTATTCGGTGATTTAGTTGAAAAATCCCGATTTATCTCTTCCGATGT
CATTGGTTCGCAATTCGAACAAATGAATTCGATGCGACTCTCGATCTCTG
ACACACAAGATCCAAATTATCAAGAAAATCTAGTTAAAGAATTTTCTATT
TTACCGGAAAATAGTTTCAACGCGGTTCCGATTGGTATTTTGAAACAGAA
ATATGATTCACAGGAAATCTGTAAAAGTTTCACTAGATTCCTTGATCCGA
AAACCGCTGTTGTCGAATATCATGTGCGGCAATTGGCTAGCAAACTGAAC
TATTACAAATGCGTGACCAATCAATGCAAAATTAACAAACCTCCCATCAA
AGAAATAAATTTCCCAAAGCAAAAATTCAACCGAATCACGAAACTAGTTG
CCAAGTTCAATCGGAATACCGCAGCAGAAAAGTCACAACTTACAATTGTA
CCTTTGATCAAGCAACCGTCACTTGAAAGAATATTTTTGAATTTATTAAA
CGTAAAATCTCTTCAAATGGTCGAAGAAATCGCTAGAGATGCGTTGAAAC
AACATCAAGTCTACAGTGTTTATCCGATGGTTATCGAGAATTGTGAGAAT
GAAAGTCCGATTCATAGTTTGTCGAATTCCAGAAATTCAGTTGTTCAACG
ATTGGAATATAACGGATCGCCGGGAGAAGTTAAAAATCAAGTAGATCGCT
CCGAAATCGTACTCCATCGAGACTGCGGAAGAATGCGATCTATAAAACAC
AGTAATACCTTTAATTATGAATTCGATAACAGCATCAATGCAAATTATAA
ATCACAATGCCTTCCGATTATCTGTAGAACTCCGTTGAAAGAAACAGAAT
CGGAAATCCAATCTCCACTGTTGGAGTTGACAGTAGACGAGTTGTTGTTG
TTGTCATCATCATCATCATCATCATCATTCAATTCCGTTAATAATGACAA
CAACCGTCTCACTGGATCGGAAACGATTTTCATCGGAAATTCTGAAACAA
TTATCAAAGAAATCAAGTCTAATTCCGAAGCAGATTTCCACGAAAATAAT
TCTCCGAAAGAAATTGATTCTACAGAGGATTATTTCTCCATGAAAATTCC
GATGAAGTTCCAAGCAGTTGAAAACAGAAATAATAATCTCGAAATAATCT
TTCGGGAAAAGATAAATTTCAATGCATCAGAAATTGAAAACCAATTGTTC
CATTTTCCCAAGCAATTCGTCTCTCCAGTGGAATCGATTTCGACAAAGCA
CTTTCCGAGTAATTGTTGTCGAGTTTCAGAGAGAGAATCTCTTTGTAAAA
TCGATTCCATTCGGAAATCCAGCAAAAGTGCCGACATTTCTATGGAAAAC
AAAACAATCAATTTCAGAAACAATCAAAACCTCACAGAATTATCATTATT
ATCATCCCAAACTGCGAACTCGACTGGATTTGTTGTCGCGGATTCTTCAC
ATATTCAATTGTCAAAGAGAGAGAAAAACTCTTTTTTCTCAAAATTTTGG
TTATTAATTGTTGCTTTGATTTTCGTTCTCGTACTCTACAAAGGTCAACT
TTCTCAGATTTGCAATTGTCTCGCATTCGGCCAATTGCTTTATCCAAAGA
TTTCATCATCTGGCCATCCAGTTTAGCAGTTGACGTTTCTCGGTCTTGAT
CAGTTATTAATTATATACTCATTTTAACTTTAATATTATTATGACGGTTA
TAAGTAAATTAATAGAAAATATATATATTCTTTGTTGAAAAAAA
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protein sequence of SMED30010117-orf-1

>SMED30010117-orf-1 ID=SMED30010117-orf-1|Name=SMED30010117-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2126bp
MNSFSYCSQISFNDCVSYELDSWTKLVKFDESQNNSKIDQSILNKHIYND
GVSKIFRQISRSKLIICCIKRQNVKIKQLIITEFKAPLLFEKIKLIDLIK
LLIYTRKEMNTILIKIEKTDSFFLSEIRNEISSLEESCEFCVEFPINENE
ITNEFCLSADVINCILNDCGLDLEELDRIVSTEIEDFENKLDPLKRSNLS
TIQKSRIERRFNKVLSVFEKDVCSNLTFISKILTRLFFGYSNLIIDPHYF
DLTPDELEKYYCELNQFQLQNIINMKSFLLDLEKKVTEYILFMKEVQMIL
SSGNVTHQEKNSLNFLENILSPIRTSSTKRNLNYELASSFLSSTPGPRRL
TKDMKSYSILSESNEIINEKSISSFDSGYLLTSGNEHSFRDSYDNFDVIP
FSDDEYEKILDDDLDIPETEEDLEFCDSICTTSEIGIPCSEINSEFSEIN
FSVSSLVADLDFDSKAFCRKNNFCKINSVGKFLFSSSGNSEFYEKNFIDI
PIVLFKRMNMMNSLVFDIELVNDLERIDRDIRQKIALLINSDEWESKYNN
VLHITLQRIRQFPLIDMSDQFEYLTTIIKSCLSTKINKMTDFETTTNVGV
QFNNSAYTTYTFCDNNIVSCEKNTNNNADSKDDDSIDLSATEHSCSINKN
DQSDSLCLDAELQVAFKTILSDSISNEHIVDTSELSNVNQLNEVAKFNNY
RDVTVNIYNTSLSDESSNEWPDHNSSDLECASQDEAKSKQTSEYIEDVKL
VVKLSELHLRSEYQSEQHNKSTLVYDKNLIVDKYKSLKSHLIYSSDDDNN
KTGDWNGNCTKSPTENRNKTNVCVQIRKSVHEPYQIENSEIIDNCDTDRD
KSEETETEFYNNINTKIQSDLYQNTEDPLQSDQPLLKELGIVNDNQSLSY
TDRISLTIDLTEKLLDLTNYRLKFISSLFNEKISSAKSTMLRNKSLTICA
LKQRLLITLKILSNLKFAIKNTYDSEIQDPMYSKIETLISKSETLTKFAV
DIENQMKTNGCDMLKNDCYNFENELPDDLLIKIEPNDLDVIVSISTCRLQ
ITIASLSQYPCYNYFQKSQLPKISKLINISLPVNCNLEDTLNPSDLLQKL
CKLRRITAICALNIVDLHVILTKLEHDGSSAHNSIENKVKNLLIVWRAVD
GECHRLLRCTSIVYHIMTDTRKVYIDLISLLAVTQSIIDTVELSKTEMDT
LKTNIESLMNYKQRILEWLEEIHLCVSVIYKKRMTNKRFKYYDEIHHFLE
ENLNTITELTDRIKNGCNQAANIVNDVITYCSNLPHCVITDIGKVLITDS
WINNKHLYYSECKTEEVNENIFHAIIDLEELTRRINNCIITKENLKSFSD
QFQEFLDQSKDIFQVINVENMDVIVSEKNNSSMIPRQIFRDSKDQSQLIF
SDESVTDNAAGKIMDKISKIAACAAENALILFIDKYELEFSDNNDTDHQI
SLLTSFSDSSDIMKEFCYRIVYNVGASLTLSIIDNILSVTQGHLLVESGM
DLKGICQDLSYRTVSTIVQQLLSDQEVMNEMFLSETSNDIANYLCEELGC
LMVNNSLQEIQVVDAPTNRNIIFISTCVSNLENKPLFGDLVEKSRFISSD
VIGSQFEQMNSMRLSISDTQDPNYQENLVKEFSILPENSFNAVPIGILKQ
KYDSQEICKSFTRFLDPKTAVVEYHVRQLASKLNYYKCVTNQCKINKPPI
KEINFPKQKFNRITKLVAKFNRNTAAEKSQLTIVPLIKQPSLERIFLNLL
NVKSLQMVEEIARDALKQHQVYSVYPMVIENCENESPIHSLSNSRNSVVQ
RLEYNGSPGEVKNQVDRSEIVLHRDCGRMRSIKHSNTFNYEFDNSINANY
KSQCLPIICRTPLKETESEIQSPLLELTVDELLLLSSSSSSSSFNSVNND
NNRLTGSETIFIGNSETIIKEIKSNSEADFHENNSPKEIDSTEDYFSMKI
PMKFQAVENRNNNLEIIFREKINFNASEIENQLFHFPKQFVSPVESISTK
HFPSNCCRVSERESLCKIDSIRKSSKSADISMENKTINFRNNQNLTELSL
LSSQTANSTGFVVADSSHIQLSKREKNSFFSKFWLLIVALIFVLVLYKGQ
LSQICNCLAFGQLLYPKISSSGHPV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000018Category 4 cell
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000101muscle cell
PLANA:0002063non-ciliated epidermis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 1141..1160