slc25a-20
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010073 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of slc25a-20 vs. Ensembl Human
Match: SLC25A19 (solute carrier family 25 member 19 [Source:HGNC Symbol;Acc:HGNC:14409]) HSP 1 Score: 228.409 bits (581), Expect = 9.190e-72 Identity = 127/300 (42.33%), Postives = 178/300 (59.33%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG++SG + R + P DV+KIRFQLQ E +S+ + Y I QAS++I EEG FWKGHVPAQ LSI +GAVQF +FE L + + Y +R + + + GG+A ATL P+DVLRTR +QGE K Y +L +A+ M EG F++GL PT++ IFP +QF Y LK Y + AE N+++ L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ ++ + +EG G FKGL PS +K+ + + FF YE F +
Sbjct: 21 AGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVDVLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLK-----------HLYKWAIPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFSYEFFCNV 309 HSP 2 Score: 53.1434 bits (126), Expect = 2.896e-7 Identity = 51/215 (23.72%), Postives = 85/215 (39.53%), Query Frame = 1
Query: 424 GYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
G + T + G V+G P DV++ R Q E KY + A ++ EEG F++G VP ++ A+QF L + + + Y R ++ H V G +A +A ++P+D+ + R QG K +++LR H++ EG +KGL P+ I + L F Y +
Sbjct: 10 GRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQF-----LSFEMLTELVHRGSVYDAREFSVHF--------VCGGLAACMATLTVHPVDVLRTRFAAQGEP-------KVYNTLR--HAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHL 202
BLAST of slc25a-20 vs. Ensembl Human
Match: SLC25A19 (solute carrier family 25 member 19 [Source:HGNC Symbol;Acc:HGNC:14409]) HSP 1 Score: 228.409 bits (581), Expect = 9.190e-72 Identity = 127/300 (42.33%), Postives = 178/300 (59.33%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG++SG + R + P DV+KIRFQLQ E +S+ + Y I QAS++I EEG FWKGHVPAQ LSI +GAVQF +FE L + + Y +R + + + GG+A ATL P+DVLRTR +QGE K Y +L +A+ M EG F++GL PT++ IFP +QF Y LK Y + AE N+++ L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ ++ + +EG G FKGL PS +K+ + + FF YE F +
Sbjct: 21 AGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVDVLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLK-----------HLYKWAIPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFSYEFFCNV 309 HSP 2 Score: 53.1434 bits (126), Expect = 2.896e-7 Identity = 51/215 (23.72%), Postives = 85/215 (39.53%), Query Frame = 1
Query: 424 GYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
G + T + G V+G P DV++ R Q E KY + A ++ EEG F++G VP ++ A+QF L + + + Y R ++ H V G +A +A ++P+D+ + R QG K +++LR H++ EG +KGL P+ I + L F Y +
Sbjct: 10 GRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQF-----LSFEMLTELVHRGSVYDAREFSVHF--------VCGGLAACMATLTVHPVDVLRTRFAAQGEP-------KVYNTLR--HAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHL 202
BLAST of slc25a-20 vs. Ensembl Human
Match: SLC25A19 (solute carrier family 25 member 19 [Source:HGNC Symbol;Acc:HGNC:14409]) HSP 1 Score: 228.409 bits (581), Expect = 9.190e-72 Identity = 127/300 (42.33%), Postives = 178/300 (59.33%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG++SG + R + P DV+KIRFQLQ E +S+ + Y I QAS++I EEG FWKGHVPAQ LSI +GAVQF +FE L + + Y +R + + + GG+A ATL P+DVLRTR +QGE K Y +L +A+ M EG F++GL PT++ IFP +QF Y LK Y + AE N+++ L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ ++ + +EG G FKGL PS +K+ + + FF YE F +
Sbjct: 21 AGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVDVLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLK-----------HLYKWAIPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFSYEFFCNV 309 HSP 2 Score: 53.1434 bits (126), Expect = 2.896e-7 Identity = 51/215 (23.72%), Postives = 85/215 (39.53%), Query Frame = 1
Query: 424 GYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
G + T + G V+G P DV++ R Q E KY + A ++ EEG F++G VP ++ A+QF L + + + Y R ++ H V G +A +A ++P+D+ + R QG K +++LR H++ EG +KGL P+ I + L F Y +
Sbjct: 10 GRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQF-----LSFEMLTELVHRGSVYDAREFSVHF--------VCGGLAACMATLTVHPVDVLRTRFAAQGEP-------KVYNTLR--HAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHL 202
BLAST of slc25a-20 vs. Ensembl Human
Match: SLC25A19 (solute carrier family 25 member 19 [Source:HGNC Symbol;Acc:HGNC:14409]) HSP 1 Score: 228.409 bits (581), Expect = 9.190e-72 Identity = 127/300 (42.33%), Postives = 178/300 (59.33%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG++SG + R + P DV+KIRFQLQ E +S+ + Y I QAS++I EEG FWKGHVPAQ LSI +GAVQF +FE L + + Y +R + + + GG+A ATL P+DVLRTR +QGE K Y +L +A+ M EG F++GL PT++ IFP +QF Y LK Y + AE N+++ L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ ++ + +EG G FKGL PS +K+ + + FF YE F +
Sbjct: 21 AGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVDVLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLK-----------HLYKWAIPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFSYEFFCNV 309 HSP 2 Score: 53.1434 bits (126), Expect = 2.896e-7 Identity = 51/215 (23.72%), Postives = 85/215 (39.53%), Query Frame = 1
Query: 424 GYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
G + T + G V+G P DV++ R Q E KY + A ++ EEG F++G VP ++ A+QF L + + + Y R ++ H V G +A +A ++P+D+ + R QG K +++LR H++ EG +KGL P+ I + L F Y +
Sbjct: 10 GRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQF-----LSFEMLTELVHRGSVYDAREFSVHF--------VCGGLAACMATLTVHPVDVLRTRFAAQGEP-------KVYNTLR--HAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHL 202
BLAST of slc25a-20 vs. Ensembl Human
Match: SLC25A19 (solute carrier family 25 member 19 [Source:HGNC Symbol;Acc:HGNC:14409]) HSP 1 Score: 228.409 bits (581), Expect = 9.190e-72 Identity = 127/300 (42.33%), Postives = 178/300 (59.33%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG++SG + R + P DV+KIRFQLQ E +S+ + Y I QAS++I EEG FWKGHVPAQ LSI +GAVQF +FE L + + Y +R + + + GG+A ATL P+DVLRTR +QGE K Y +L +A+ M EG F++GL PT++ IFP +QF Y LK Y + AE N+++ L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ ++ + +EG G FKGL PS +K+ + + FF YE F +
Sbjct: 21 AGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVDVLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLK-----------HLYKWAIPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFSYEFFCNV 309 HSP 2 Score: 53.1434 bits (126), Expect = 2.896e-7 Identity = 51/215 (23.72%), Postives = 85/215 (39.53%), Query Frame = 1
Query: 424 GYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
G + T + G V+G P DV++ R Q E KY + A ++ EEG F++G VP ++ A+QF L + + + Y R ++ H V G +A +A ++P+D+ + R QG K +++LR H++ EG +KGL P+ I + L F Y +
Sbjct: 10 GRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQF-----LSFEMLTELVHRGSVYDAREFSVHF--------VCGGLAACMATLTVHPVDVLRTRFAAQGEP-------KVYNTLR--HAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHL 202
BLAST of slc25a-20 vs. Ensembl Celegans
Match: hpo-12 (pep chromosome:WBcel235:IV:12283174:12285065:1 gene:WBGene00016588.1 transcript:C42C1.10.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:hpo-12) HSP 1 Score: 211.075 bits (536), Expect = 1.464e-65 Identity = 128/305 (41.97%), Postives = 170/305 (55.74%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTG-------YSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ-GEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKF-HHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
AG SG + R+I QPLDVLKIRFQLQ EPI + K YK + Q+ I EEG FWKGH+PAQ LS +G VQF +FE L + S ST++ G ++G A P+DV+RTRLV+Q + Y +A+ + +EG+ G+FRG VP+V+ I P T +QF +Y+ M + Y +S AL SG++AG VAKTV+YPLD+ + RLQ+ GFE R FGK ++S + +I V E GLFKGL PS IK+ S F FYE F +I
Sbjct: 21 AGLASGIVTRMIIQPLDVLKIRFQLQEEPI-RGKKSGKYKGVMQSIFLITREEGAHAFWKGHIPAQGLSATYGLVQFSSFEWLSQQAAKVIPADNQSVRSTSDFACGALSGCLAMTAAMPLDVIRTRLVAQKAGHAVYTGTMHAVKHIWEKEGIAGYFRGWVPSVVQIAPFTGMQFALYNCF------MDLWPFNGY-----------ESAGALFSGAMAGTVAKTVLYPLDMVRHRLQMNGFE--RAGFGKTSNYSQGLFKTIGMVVKNESWYGLFKGLWPSQIKAAANSGCAFLFYEIFCDLI 305 HSP 2 Score: 71.633 bits (174), Expect = 9.574e-14 Identity = 53/188 (28.19%), Postives = 83/188 (44.15%), Query Frame = 1
Query: 145 TNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF---RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQG----EYKKYKSLTNAILK----MSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIY 675
+ S F GALSG + PLDV++ R +++ + Y A K I+ +EGI G+++G VP+ F +QF F L+ GY S L G +AG+ A P+D++R RL G + K + + + K + E YG F+GL P+ + + F Y
Sbjct: 119 STSDFACGALSGCLAMTAAMPLDVIRTRL------VAQKAGHAVYTGTMHAVKHIWEKEGIAGYFRGWVPSVVQIAPFTGMQFALYNCFMDLWPFNGYE--SAGALFSGAMAGTVAKTVLYPLDMVRHRLQMNGFERAGFGKTSNYSQGLFKTIGMVVKNESWYGLFKGLWPSQIKAAANSGCAFLFY 298
BLAST of slc25a-20 vs. Ensembl Celegans
Match: hpo-12 (pep chromosome:WBcel235:IV:12283174:12285122:1 gene:WBGene00016588.1 transcript:C42C1.10.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:hpo-12) HSP 1 Score: 211.075 bits (536), Expect = 1.464e-65 Identity = 128/305 (41.97%), Postives = 170/305 (55.74%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTG-------YSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ-GEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKF-HHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
AG SG + R+I QPLDVLKIRFQLQ EPI + K YK + Q+ I EEG FWKGH+PAQ LS +G VQF +FE L + S ST++ G ++G A P+DV+RTRLV+Q + Y +A+ + +EG+ G+FRG VP+V+ I P T +QF +Y+ M + Y +S AL SG++AG VAKTV+YPLD+ + RLQ+ GFE R FGK ++S + +I V E GLFKGL PS IK+ S F FYE F +I
Sbjct: 21 AGLASGIVTRMIIQPLDVLKIRFQLQEEPI-RGKKSGKYKGVMQSIFLITREEGAHAFWKGHIPAQGLSATYGLVQFSSFEWLSQQAAKVIPADNQSVRSTSDFACGALSGCLAMTAAMPLDVIRTRLVAQKAGHAVYTGTMHAVKHIWEKEGIAGYFRGWVPSVVQIAPFTGMQFALYNCF------MDLWPFNGY-----------ESAGALFSGAMAGTVAKTVLYPLDMVRHRLQMNGFE--RAGFGKTSNYSQGLFKTIGMVVKNESWYGLFKGLWPSQIKAAANSGCAFLFYEIFCDLI 305 HSP 2 Score: 71.633 bits (174), Expect = 9.574e-14 Identity = 53/188 (28.19%), Postives = 83/188 (44.15%), Query Frame = 1
Query: 145 TNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF---RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQG----EYKKYKSLTNAILK----MSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIY 675
+ S F GALSG + PLDV++ R +++ + Y A K I+ +EGI G+++G VP+ F +QF F L+ GY S L G +AG+ A P+D++R RL G + K + + + K + E YG F+GL P+ + + F Y
Sbjct: 119 STSDFACGALSGCLAMTAAMPLDVIRTRL------VAQKAGHAVYTGTMHAVKHIWEKEGIAGYFRGWVPSVVQIAPFTGMQFALYNCFMDLWPFNGYE--SAGALFSGAMAGTVAKTVLYPLDMVRHRLQMNGFERAGFGKTSNYSQGLFKTIGMVVKNESWYGLFKGLWPSQIKAAANSGCAFLFY 298
BLAST of slc25a-20 vs. Ensembl Celegans
Match: R07E3.4 (pep chromosome:WBcel235:X:10330547:10331900:-1 gene:WBGene00011105.1 transcript:R07E3.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:R07E3.4) HSP 1 Score: 100.138 bits (248), Expect = 8.836e-24 Identity = 80/317 (25.24%), Postives = 142/317 (44.79%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGH-------VPAQYLSIVFGAVQFRTF-EALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKT---VIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVL-EEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSK 1044
+ T+ AG+ + I + T P D +K+ QLQ + +F + Y I+ +I LE+G W+G+ +P L+ F + T + + + + + GG+ G+T P D RTRL V + +KYK + + + K+ A EGV +++GL + + AI FGI+ +++S+ + S+N ++A I T V YPLD +R + +Q + +QY KT+ ++GI+G ++G L +S++S + + F+YE FSK
Sbjct: 11 LETSKKCLAGSAAAAISKTTTAPFDRVKLVLQLQRQS---EFAMAEYNGIRDCISKIRLEQGAMALWRGNGAGVARCLPNHTLNFAFRDIYRNTLLKNVDRNESFGKFLAGTFVSGGLGGATTLFMLYPFDFARTRLALDVKKDGSRKYKGMVDCLKKIKASEGVASWYKGLSSALQFVIASRAIFFGIFDSIRTSVEDPK-------------------SLNFAACWAIAQISITTSGMVCYPLDTVRRSMMMQSGKQIKQYTS--------TKDCWKTLYKKDGINGFYRGALTNSLRSTGGALIITFYYE-FSK 296
BLAST of slc25a-20 vs. Ensembl Celegans
Match: slc-25A42 (SLC (SoLute Carrier) homolog [Source:UniProtKB/TrEMBL;Acc:Q93717]) HSP 1 Score: 98.5969 bits (244), Expect = 2.869e-23 Identity = 82/308 (26.62%), Postives = 135/308 (43.83%), Query Frame = 1
Query: 154 AFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQV----QGFEDARQYFGKFHHSLRMVHSIRKTVLEEGIS-GLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
+ +AGA++G + + PLD KI FQ +S S+ IK K Y E G F ++G+ + + ++QF FE L SRT + G +A +TAT+ P+D + RL S +Y SL + +K E G+ +RG+ PT+L + P F Y LK R + +Y ++ G +AG++ ++ YPLD+ +RR+Q G+ R +H EG+ GL+KGL + +K + ++F YEK +++
Sbjct: 17 SLSAGAIAGALAKTTIAPLDRTKIYFQ-----VSSTRGYSFRSAIKFI-KLTYRENGFFALYRGNSATMARVVPYASMQFAAFEQYKKLLKVDENGSRTPVKRYITGSLAATTATMITYPLDTAKARL-SVSSKLQYSSLKHVFVKTYKEGGIQLLYRGIYPTILGVIPYAGSSFFTYETLKIMYRDHRGEVENSYY--------------RMLFGMLAGLIGQSSSYPLDIVRRRMQTGRIPSGWSPLRALIHIYH--------------TEGLKRGLYKGLSMNWLKGPIAVGVSFTTYEKVLELV 289
BLAST of slc25a-20 vs. Ensembl Celegans
Match: slc-25A32 (SLC (SoLute Carrier) homolog [Source:UniProtKB/TrEMBL;Acc:A5JYR3]) HSP 1 Score: 97.0561 bits (240), Expect = 9.215e-23 Identity = 82/321 (25.55%), Postives = 145/321 (45.17%), Query Frame = 1
Query: 124 FKVLHMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTST-----NNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY---KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDS----VNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
K+ ++ G G ++ P D+LKIRF + SY A ++I EG+ G ++G P+ + + + F+ + +L +K Y ST NNL+ G ++GS PI + +TRL Q E KKY + + + K +EG +G +RG V V+ A+Q Y S + R QS + L DS + +++ S + ++A T+ +P + + R+Q D+R G + +L+ +H+ EGI GL+KG L ++++ + +TF YE +++
Sbjct: 1 MKIPDLTNYEHLIGGLCGGVTSTVVCHPFDLLKIRFSANEGSSLRPQYSSY----ADAVRKIVRVEGVRGLYQGWTPSLIGASLSWGLYFQWYNSLRTKI-YENFSTGSKLANNLISGCISGSAIMCITNPIWLTKTRLCLQYENQQSKKYAGMMDCLKKTVKQEGFFGLYRGFVTGVIGT-THGAVQIAAY----SWIIDKRCQS----------QGLPKDSFLSQTDYVIASSTSKVLATTITFPYQVLRTRMQDHN-TDSR---GVWKTTLKTIHN-------EGIGGLWKGCLIANVRQLPAAVVTFLTYENVKRLV 290
BLAST of slc25a-20 vs. Ensembl Fly
Match: Tpc1 (gene:FBgn0037852 transcript:FBtr0082334) HSP 1 Score: 202.986 bits (515), Expect = 3.795e-62 Identity = 121/307 (39.41%), Postives = 171/307 (55.70%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKD-------SYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL---YSKTGY--SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG LS I R QPLDVLKIRFQLQVEP+ K+ S Y +I QA K IY EEG+ FWKGH PAQ LSI++G QF T+E L +T Y +N L G AG A + P+DV+RTRL++Q K Y++ T A+ + +EG G +RGL +L I P F Y R+ S+ ++ + L G+ +G+++KT++YP DL K+RLQ+QGFE RQ FG+ + +R TV +EG+ GL+KG+ P+ +KS + ++L F Y+K ++
Sbjct: 34 AGGLSAAITRSTCQPLDVLKIRFQLQVEPLGKNAAKEGPGALTSKYTSIGQAVKTIYREEGMLAFWKGHNPAQVLSIMYGICQFWTYEQLSLMAKQTSYLADHQHLSNFLCGAAAGGAAVIISTPLDVIRTRLIAQDTSKGYRNATRAVSAIVRQEGPRGMYRGLSSALLQITPLMGTNFMAY----------RLFSDWACAFLEVSDRSQLPTWTLLGLGASSGMLSKTIVYPFDLIKKRLQIQGFESNRQTFGQTLQCHGVWDCLRLTVRQEGVRGLYKGVAPTLLKSSMTTALYFSIYDKLKQV 330 HSP 2 Score: 54.6842 bits (130), Expect = 5.867e-8 Identity = 56/218 (25.69%), Postives = 94/218 (43.12%), Query Frame = 1
Query: 430 SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGE--------------YKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFS 1041
+R + +L GG++ + CQP+DVL+ R Q E KY S+ A+ + EEG+ F++G P ++ QF Y QL + + Q + + +HLSN + G+ AG A + PLD+ + RL Q + ++ R V +I V +EG G+++GL + ++ + F Y FS
Sbjct: 25 TREQLHQMLAGGLSAAITRSTCQPLDVLKIRFQLQVEPLGKNAAKEGPGALTSKYTSIGQAVKTIYREEGMLAFWKGHNPAQVLSIMYGICQFWTYEQLS-------LMAKQTSYLADH-QHLSN-----FLCGAAAGGAAVIISTPLDVIRTRLIAQDTSKG------YRNATRAVSAI---VRQEGPRGMYRGLSSALLQITPLMGTNFMAYRLFS 220
BLAST of slc25a-20 vs. Ensembl Fly
Match: Tpc1 (gene:FBgn0037852 transcript:FBtr0082333) HSP 1 Score: 202.986 bits (515), Expect = 3.795e-62 Identity = 121/307 (39.41%), Postives = 171/307 (55.70%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKD-------SYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL---YSKTGY--SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG LS I R QPLDVLKIRFQLQVEP+ K+ S Y +I QA K IY EEG+ FWKGH PAQ LSI++G QF T+E L +T Y +N L G AG A + P+DV+RTRL++Q K Y++ T A+ + +EG G +RGL +L I P F Y R+ S+ ++ + L G+ +G+++KT++YP DL K+RLQ+QGFE RQ FG+ + +R TV +EG+ GL+KG+ P+ +KS + ++L F Y+K ++
Sbjct: 34 AGGLSAAITRSTCQPLDVLKIRFQLQVEPLGKNAAKEGPGALTSKYTSIGQAVKTIYREEGMLAFWKGHNPAQVLSIMYGICQFWTYEQLSLMAKQTSYLADHQHLSNFLCGAAAGGAAVIISTPLDVIRTRLIAQDTSKGYRNATRAVSAIVRQEGPRGMYRGLSSALLQITPLMGTNFMAY----------RLFSDWACAFLEVSDRSQLPTWTLLGLGASSGMLSKTIVYPFDLIKKRLQIQGFESNRQTFGQTLQCHGVWDCLRLTVRQEGVRGLYKGVAPTLLKSSMTTALYFSIYDKLKQV 330 HSP 2 Score: 54.6842 bits (130), Expect = 5.867e-8 Identity = 56/218 (25.69%), Postives = 94/218 (43.12%), Query Frame = 1
Query: 430 SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGE--------------YKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFS 1041
+R + +L GG++ + CQP+DVL+ R Q E KY S+ A+ + EEG+ F++G P ++ QF Y QL + + Q + + +HLSN + G+ AG A + PLD+ + RL Q + ++ R V +I V +EG G+++GL + ++ + F Y FS
Sbjct: 25 TREQLHQMLAGGLSAAITRSTCQPLDVLKIRFQLQVEPLGKNAAKEGPGALTSKYTSIGQAVKTIYREEGMLAFWKGHNPAQVLSIMYGICQFWTYEQLS-------LMAKQTSYLADH-QHLSN-----FLCGAAAGGAAVIISTPLDVIRTRLIAQDTSKG------YRNATRAVSAI---VRQEGPRGMYRGLSSALLQITPLMGTNFMAYRLFS 220
BLAST of slc25a-20 vs. Ensembl Fly
Match: Tpc2 (gene:FBgn0035078 transcript:FBtr0304800) HSP 1 Score: 171.4 bits (433), Expect = 4.846e-50 Identity = 99/292 (33.90%), Postives = 161/292 (55.14%), Query Frame = 1
Query: 190 RLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKT---GYSRTSTNNLLF--GGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTN--AILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSK 1044
R ITQPLDVLKIRFQ+QVEP++ + K S Y+ + A K +Y EEG+ G ++GH Q LSI + VQF ++E L S Y R + F GG+AG + QP DV+RT++V+ + + + K+ EG G RGL T++ +FP F Y L +++ + + + + ++G+++G++AK ++YP DL K+R+Q+ F+ R+ FG+ ++ I T EEGI G +KG+LP+ +K+G++S++ F Y+ F +
Sbjct: 24 RTITQPLDVLKIRFQMQVEPVT-NHKGSKYRGVIHAFKSVYAEEGMRGMFRGHNSGQVLSISYALVQFWSYEQLRSMAHQFDYWRERPFLMFFICGGIAGCLGAVAAQPFDVVRTQMVAADPSSRRSQMNTFTGLRKVYKMEGWMGLSRGLPFTLVQVFPLVGANFLFYKYLNAAVLMAKPPDQRQEIHGAFL----------FLNGALSGVLAKMIVYPADLLKKRIQLMAFKQERKTFGRNPECPTILGCITTTFREEGIGGFYKGMLPTLLKAGLMSAVYFSIYDMFKR 304
BLAST of slc25a-20 vs. Ensembl Fly
Match: DPCoAC (gene:FBgn0067783 transcript:FBtr0344281) HSP 1 Score: 104.375 bits (259), Expect = 2.890e-25 Identity = 85/299 (28.43%), Postives = 132/299 (44.15%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVE-PISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVS---GSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYE 1032
+GA +G + + + PLD KI FQ++ + P S Y +N Y EG+ W+G+ + + A+QF E L+ + T L G +AG T+ P+D+ R R+ Y Y++L K+ EEG FRG TVL + P F Y LK R Y E + N+ N LVS G+ AG +T YPLD+ +RR+Q A G + ++ + ++ K EEG+ +G +KGL + IK + ++F Y+
Sbjct: 3 SGAAAGALAKTVIAPLDRTKINFQIRNDVPFSFRASLRYLQNT-------YANEGVLALWRGNSATMARIVPYAAIQFTAHEQWRRILHVDKDGTNTKGRRFLAGSLAGITSQSLTYPLDLARARMAVTDRYTGYRTLRQVFTKIWVEEGPRTLFRGYWATVLGVIPYAGTSFFTYETLK----------------REYYEVVGNNKPNTLVSLAFGAAAGAAGQTASYPLDIVRRRMQTMRVNTA---GGDRYPTI--LETLVKIYREEGVKNGFYKGLSMNWIKGPIAVGISFSTYD 273
BLAST of slc25a-20 vs. Ensembl Fly
Match: DPCoAC (gene:FBgn0067783 transcript:FBtr0089481) HSP 1 Score: 104.375 bits (259), Expect = 2.890e-25 Identity = 85/299 (28.43%), Postives = 132/299 (44.15%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVE-PISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVS---GSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYE 1032
+GA +G + + + PLD KI FQ++ + P S Y +N Y EG+ W+G+ + + A+QF E L+ + T L G +AG T+ P+D+ R R+ Y Y++L K+ EEG FRG TVL + P F Y LK R Y E + N+ N LVS G+ AG +T YPLD+ +RR+Q A G + ++ + ++ K EEG+ +G +KGL + IK + ++F Y+
Sbjct: 3 SGAAAGALAKTVIAPLDRTKINFQIRNDVPFSFRASLRYLQNT-------YANEGVLALWRGNSATMARIVPYAAIQFTAHEQWRRILHVDKDGTNTKGRRFLAGSLAGITSQSLTYPLDLARARMAVTDRYTGYRTLRQVFTKIWVEEGPRTLFRGYWATVLGVIPYAGTSFFTYETLK----------------REYYEVVGNNKPNTLVSLAFGAAAGAAGQTASYPLDIVRRRMQTMRVNTA---GGDRYPTI--LETLVKIYREEGVKNGFYKGLSMNWIKGPIAVGISFSTYD 273
BLAST of slc25a-20 vs. Ensembl Zebrafish
Match: slc25a19 (solute carrier family 25 member 19 [Source:ZFIN;Acc:ZDB-GENE-050417-292]) HSP 1 Score: 245.743 bits (626), Expect = 8.316e-79 Identity = 131/309 (42.39%), Postives = 189/309 (61.17%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
++ A AG+ +G + R + PLDV+KIRFQLQ+E +S + Y + QA++ I EEG+ FWKGH+PAQ LS+ +GAVQF +FE L + KT Y S+T+ + + GG+A +AT+ CQP+D LRTR +QGE K Y++L +AI M EG + F+RGL PT++ +FP +QF Y+ LK L +Q + S +++L+SGS AG+++KT+ YP DL K+RLQV GFE+AR FG+ V + + EEG G FKGL PS +K+ + + TFF+YE F I
Sbjct: 13 LAPEEAALAGSAAGIVTRALISPLDVVKIRFQLQIEKVSWRSRQGKYWGLWQATRCILTEEGLPAFWKGHIPAQLLSVCYGAVQFASFEVLTELVHKKTPYNSQTAGVHFICGGLAACSATVACQPLDTLRTRFAAQGEPKIYRNLRHAIGTMLRSEGPFTFYRGLTPTLVAVFPYAGLQFFFYNILKKLL---ELQDTK-----------SKAGLHSLISGSCAGVISKTLTYPFDLIKKRLQVGGFEEARLKFGEVRTYHGFVDCVLRIGREEGPRGFFKGLSPSLLKAALSTGFTFFWYEFFISAI 307 HSP 2 Score: 77.411 bits (189), Expect = 1.898e-15 Identity = 52/197 (26.40%), Postives = 87/197 (44.16%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYS----KTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---------VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMR 708
F G L+ + QPLD L+ RF Q EP Y+N++ A + EG F F++G P + +QF + L + S+ ++L+ G AG + P D+++ RL + GE + Y + +L++ EEG GFF+GL P++L T F Y S++ +++
Sbjct: 122 FICGGLAACSATVACQPLDTLRTRFAAQGEP-------KIYRNLRHAIGTMLRSEGPFTFYRGLTPTLVAVFPYAGLQFFFYNILKKLLELQDTKSKAGLHSLISGSCAGVISKTLTYPFDLIKKRLQVGGFEEARLKFGEVRTYHGFVDCVLRIGREEGPRGFFKGLSPSLLKAALSTGFTFFWYEFFISAIVTLK 311
BLAST of slc25a-20 vs. Ensembl Zebrafish
Match: slc25a42 (solute carrier family 25 member 42 [Source:NCBI gene;Acc:751743]) HSP 1 Score: 115.931 bits (289), Expect = 3.744e-29 Identity = 83/308 (26.95%), Postives = 143/308 (46.43%), Query Frame = 1
Query: 142 STNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFE-------ALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSND---SVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYE 1032
S ++ +GA +G + + PLD KI FQ+ S K++Y + R YL++G F W+G+ I + A+QF E Y G + LL G +AG+TA + P+D++R R+ + + Y ++ + +++S EEG+ +RG PT+L + P + F Y LK + +AE LV G+ AG++ ++ YPLD+ +RR+Q G H ++ ++R+ V EEGI GL+KGL + +K + ++F ++
Sbjct: 39 SVLNSLVSGAFAGAVAKTAVAPLDRTKIIFQVSSNRFSA--KEAY-----RLIYRTYLKDGFFSLWRGNSATMVRVIPYAAIQFCAHEQYKGILGKYYGFQGKALPPVPRLLAGSLAGTTAAIITYPLDMVRARMAVTPK-EMYSNIMDVFVRISREEGLKTLYRGFTPTILGVVPYAGLSFFTYETLK----------------KTHAEKTGRAHPFPYERLVFGACAGLIGQSASYPLDVVRRRMQTAGVTG--------HTYSTVLGTMREIVAEEGIVRGLYKGLSMNWVKGPIAVGISFMTFD 314 HSP 2 Score: 66.2402 bits (160), Expect = 1.009e-11 Identity = 55/207 (26.57%), Postives = 91/207 (43.96%), Query Frame = 1
Query: 433 RTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY--KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYA-EHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSK 1044
R+ N+L+ G AG+ A P+D RT+++ Q ++ K I + ++G + +RG T++ + P AIQF + Q K L + Y + + V L++GS+AG A + YPLD+ + R+ V E F R EEG+ L++G P+ + + L+FF YE K
Sbjct: 38 RSVLNSLVSGAFAGAVAKTAVAPLD--RTKIIFQVSSNRFSAKEAYRLIYRTYLKDGFFSLWRGNSATMVRVIPYAAIQFCAHEQYKGILG------------KYYGFQGKALPPVPRLLAGSLAGTTAAIITYPLDMVRARMAVTPKEMYSNIMDVFVRISR----------EEGLKTLYRGFTPTILGVVPYAGLSFFTYETLKK 220
BLAST of slc25a-20 vs. Ensembl Zebrafish
Match: slc25a16 (solute carrier family 25 member 16 [Source:ZFIN;Acc:ZDB-GENE-030131-3299]) HSP 1 Score: 107.457 bits (267), Expect = 5.435e-26 Identity = 90/308 (29.22%), Postives = 147/308 (47.73%), Query Frame = 1
Query: 154 AFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ-GEYKKYKSLTNAILKMSAEEG-VYGFFRGLVPTVLMIFPQTAIQFGIYHQLKS--------SLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYE 1032
+FTAG ++G + PLD +KI Q Q P K + IK K+ EG G +KG+ +GA+QF F+ L++K G S + L+ G +AG TA + P+DV+R RL Q + +Y + +A + +EG + GF+RGL+PT++ + P F + LK+ L + + V++ VN L+ G VAG +A+T+ YPLD+A+RR+Q+ +L+ V+S + G+ GL++GL + I+ ++ F YE
Sbjct: 28 SFTAGGVAGCCAKSTIAPLDRVKILLQAQ-NPHYKHL--GVFATIKAVPKK----EGFLGLYKGNGAMMIRIFPYGAIQFMAFDNYKKFLHTKVGIS-GHVHRLMAGSMAGMTAVICTYPLDVIRARLAFQVTGHHRYSGIRHAFQTIYHKEGGISGFYRGLIPTIIGMAPYAGFSFFTFGTLKTLGLTHFPEQLGKPSLDNPDVLVLKT--------QVN-LLCGGVAGAIAQTISYPLDVARRRMQLGASLPDHDKCCSLTKTLKHVYS------QYGVKKGLYRGLSLNYIRCVPSQAVAFTTYE 312
BLAST of slc25a-20 vs. Ensembl Zebrafish
Match: slc25a16 (solute carrier family 25 member 16 [Source:ZFIN;Acc:ZDB-GENE-030131-3299]) HSP 1 Score: 106.686 bits (265), Expect = 7.957e-26 Identity = 88/310 (28.39%), Postives = 149/310 (48.06%), Query Frame = 1
Query: 154 AFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIK--QASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ-GEYKKYKSLTNAILKMSAEEG-VYGFFRGLVPTVLMIFPQTAIQFGIYHQLKS--------SLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYE 1032
+FTAG ++G + PLD +KI Q Q + +YK++ K + +EG G +KG+ +GA+QF F+ L++K G S + L+ G +AG TA + P+DV+R RL Q + +Y + +A + +EG + GF+RGL+PT++ + P F + LK+ L + + V++ VN L+ G VAG +A+T+ YPLD+A+RR+Q+ +L+ V+S + G+ GL++GL + I+ ++ F YE
Sbjct: 28 SFTAGGVAGCCAKSTIAPLDRVKILLQAQ---------NPHYKHLGVFATLKAVPKKEGFLGLYKGNGAMMIRIFPYGAIQFMAFDNYKKFLHTKVGIS-GHVHRLMAGSMAGMTAVICTYPLDVIRARLAFQVTGHHRYTGIRHAFQTIYHKEGGISGFYRGLIPTIIGMAPYAGFSFFTFGTLKTLGLTHFPEQLGKPSLDNPDVLVLKT--------QVN-LLCGGVAGAIAQTISYPLDVARRRMQLGASLPDHDKCCSLTKTLKHVYS------QYGVKKGLYRGLSLNYIRCVPSQAVAFTTYE 312
BLAST of slc25a-20 vs. Ensembl Zebrafish
Match: slc25a5 (solute carrier family 25 member 5 [Source:ZFIN;Acc:ZDB-GENE-020419-9]) HSP 1 Score: 102.449 bits (254), Expect = 2.298e-24 Identity = 89/313 (28.43%), Postives = 142/313 (45.37%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDF-KDSYYKNIKQASKRIYLEEGIFGFWKGHV-------PAQYLSIVFGAVQFRTF-EALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ----GEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQ-GFEDAR-QYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
F AG ++ I + P++ +++ LQV+ SK D YK I RI E+G FW+G++ P Q L+ F + F + + +T + R NL GG AG+T+ F P+D RTRL + G +++ L N ++K+S +G+ G ++G +V I A FGIY K L + N + V+ + SV A VAG+ + YP D +RR+ +Q G + A Y G + RK +EG FKG S++ G+ + Y++ K+I
Sbjct: 12 FLAGGIAAAISKTAVAPIE--RVKLLLQVQHASKQITADKQYKGIMDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKKVFLDGVDKRTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFSGLGNCLVKISKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPK---NTHIVV----SWMIAQSVTA-----VAGLAS----YPFDTVRRRMMMQSGRKGADIMYSG-------TIDCWRKIARDEGGKAFFKGAW-SNVLRGMGGAFVLVLYDELKKVI 298 HSP 2 Score: 60.077 bits (144), Expect = 9.714e-10 Identity = 47/187 (25.13%), Postives = 83/187 (44.39%), Query Frame = 1
Query: 442 TNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY--------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQ-GFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGL 975
+ L GG+A + + PI+ R +L+ Q ++ K+YK + + ++++ E+G F+RG + V+ FP A+ F + K + Q + R +A +L+ SG AG + +YPLD A+ RL G A + F + L + +GI GL++G
Sbjct: 9 AKDFLAGGIAAAISKTAVAPIE--RVKLLLQVQHASKQITADKQYKGIMDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKKVFLDGVDKRTQFW--RYFAGNLA--------SGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFSGLGNCLVKISK------SDGIKGLYQGF 177
BLAST of slc25a-20 vs. Ensembl Xenopus
Match: slc25a19 (solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 [Source:NCBI gene;Acc:548707]) HSP 1 Score: 249.98 bits (637), Expect = 2.858e-80 Identity = 136/309 (44.01%), Postives = 182/309 (58.90%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL-----YSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
+S++ AG+LSG + R + PLDV+KIRFQLQ+E +S Y I QA I EEG+ GFWKGHVPAQ LS+ +GAVQF +FE L S + R+ + L GG+A +ATL QP+D LRTR +QGE K Y++L NAI M EG F+RGL PT+L +FP +QF Y+ LK + + + + + DS+ L+ GS AG+++KTV YP DL K+RLQV GFE AR +FGK +V + EEG G FKGL PS +K+ + LTFF YE F ++
Sbjct: 13 LSSSEVAMAGSLSGLVTRALISPLDVIKIRFQLQIESLSSHGTQGKYHGILQAVGLILREEGLPGFWKGHVPAQLLSVSYGAVQFVSFEMLTELFHVSTSLDPRSPAVHFLCGGLAACSATLAVQPLDTLRTRFAAQGEPKVYRNLRNAIFTMFRTEGPVAFYRGLFPTLLAVFPYAGLQFSSYNLLK-----------RTWNLVLLKDQTQKDSLRNLLCGSGAGVISKTVTYPFDLFKKRLQVGGFEQARAHFGKVRTYHGLVDCACQIWKEEGFRGFFKGLAPSLLKAAFSTGLTFFSYELFCSLM 310 HSP 2 Score: 77.7962 bits (190), Expect = 2.269e-15 Identity = 61/225 (27.11%), Postives = 94/225 (41.78%), Query Frame = 1
Query: 73 MLLIVYFINSFIY*ITLFKVLHMSTN-------SAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF-------RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---------VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYH 678
+L + Y F+ L ++ H+ST+ F G L+ L QPLD L+ RF Q EP Y+N++ A ++ EG F++G P + +QF RT+ + K + S NLL G AG + P D+ + RL G+ + Y L + ++ EEG GFF+GL P++L T + F Y
Sbjct: 87 LLSVSYGAVQFVSFEMLTELFHVSTSLDPRSPAVHFLCGGLAACSATLAVQPLDTLRTRFAAQGEP-------KVYRNLRNAIFTMFRTEGPVAFYRGLFPTLLAVFPYAGLQFSSYNLLKRTWNLVLLKDQTQKDSLRNLLCGSGAGVISKTVTYPFDLFKKRLQVGGFEQARAHFGKVRTYHGLVDCACQIWKEEGFRGFFKGLAPSLLKAAFSTGLTFFSYE 304
BLAST of slc25a-20 vs. Ensembl Xenopus
Match: slc25a19 (solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 [Source:NCBI gene;Acc:548707]) HSP 1 Score: 249.98 bits (637), Expect = 4.048e-80 Identity = 136/309 (44.01%), Postives = 182/309 (58.90%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL-----YSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
+S++ AG+LSG + R + PLDV+KIRFQLQ+E +S Y I QA I EEG+ GFWKGHVPAQ LS+ +GAVQF +FE L S + R+ + L GG+A +ATL QP+D LRTR +QGE K Y++L NAI M EG F+RGL PT+L +FP +QF Y+ LK + + + + + DS+ L+ GS AG+++KTV YP DL K+RLQV GFE AR +FGK +V + EEG G FKGL PS +K+ + LTFF YE F ++
Sbjct: 18 LSSSEVAMAGSLSGLVTRALISPLDVIKIRFQLQIESLSSHGTQGKYHGILQAVGLILREEGLPGFWKGHVPAQLLSVSYGAVQFVSFEMLTELFHVSTSLDPRSPAVHFLCGGLAACSATLAVQPLDTLRTRFAAQGEPKVYRNLRNAIFTMFRTEGPVAFYRGLFPTLLAVFPYAGLQFSSYNLLK-----------RTWNLVLLKDQTQKDSLRNLLCGSGAGVISKTVTYPFDLFKKRLQVGGFEQARAHFGKVRTYHGLVDCACQIWKEEGFRGFFKGLAPSLLKAAFSTGLTFFSYELFCSLM 315 HSP 2 Score: 77.411 bits (189), Expect = 2.611e-15 Identity = 61/225 (27.11%), Postives = 94/225 (41.78%), Query Frame = 1
Query: 73 MLLIVYFINSFIY*ITLFKVLHMSTN-------SAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF-------RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---------VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYH 678
+L + Y F+ L ++ H+ST+ F G L+ L QPLD L+ RF Q EP Y+N++ A ++ EG F++G P + +QF RT+ + K + S NLL G AG + P D+ + RL G+ + Y L + ++ EEG GFF+GL P++L T + F Y
Sbjct: 92 LLSVSYGAVQFVSFEMLTELFHVSTSLDPRSPAVHFLCGGLAACSATLAVQPLDTLRTRFAAQGEP-------KVYRNLRNAIFTMFRTEGPVAFYRGLFPTLLAVFPYAGLQFSSYNLLKRTWNLVLLKDQTQKDSLRNLLCGSGAGVISKTVTYPFDLFKKRLQVGGFEQARAHFGKVRTYHGLVDCACQIWKEEGFRGFFKGLAPSLLKAAFSTGLTFFSYE 309
BLAST of slc25a-20 vs. Ensembl Xenopus
Match: slc25a19 (solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 [Source:NCBI gene;Acc:548707]) HSP 1 Score: 249.595 bits (636), Expect = 1.063e-79 Identity = 136/309 (44.01%), Postives = 182/309 (58.90%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL-----YSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
+S++ AG+LSG + R + PLDV+KIRFQLQ+E +S Y I QA I EEG+ GFWKGHVPAQ LS+ +GAVQF +FE L S + R+ + L GG+A +ATL QP+D LRTR +QGE K Y++L NAI M EG F+RGL PT+L +FP +QF Y+ LK + + + + + DS+ L+ GS AG+++KTV YP DL K+RLQV GFE AR +FGK +V + EEG G FKGL PS +K+ + LTFF YE F ++
Sbjct: 42 LSSSEVAMAGSLSGLVTRALISPLDVIKIRFQLQIESLSSHGTQGKYHGILQAVGLILREEGLPGFWKGHVPAQLLSVSYGAVQFVSFEMLTELFHVSTSLDPRSPAVHFLCGGLAACSATLAVQPLDTLRTRFAAQGEPKVYRNLRNAIFTMFRTEGPVAFYRGLFPTLLAVFPYAGLQFSSYNLLK-----------RTWNLVLLKDQTQKDSLRNLLCGSGAGVISKTVTYPFDLFKKRLQVGGFEQARAHFGKVRTYHGLVDCACQIWKEEGFRGFFKGLAPSLLKAAFSTGLTFFSYELFCSLM 339 HSP 2 Score: 77.411 bits (189), Expect = 2.954e-15 Identity = 61/224 (27.23%), Postives = 94/224 (41.96%), Query Frame = 1
Query: 73 MLLIVYFINSFIY*ITLFKVLHMSTN-------SAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF-------RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---------VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIY 675
+L + Y F+ L ++ H+ST+ F G L+ L QPLD L+ RF Q EP Y+N++ A ++ EG F++G P + +QF RT+ + K + S NLL G AG + P D+ + RL G+ + Y L + ++ EEG GFF+GL P++L T + F Y
Sbjct: 116 LLSVSYGAVQFVSFEMLTELFHVSTSLDPRSPAVHFLCGGLAACSATLAVQPLDTLRTRFAAQGEP-------KVYRNLRNAIFTMFRTEGPVAFYRGLFPTLLAVFPYAGLQFSSYNLLKRTWNLVLLKDQTQKDSLRNLLCGSGAGVISKTVTYPFDLFKKRLQVGGFEQARAHFGKVRTYHGLVDCACQIWKEEGFRGFFKGLAPSLLKAAFSTGLTFFSY 332
BLAST of slc25a-20 vs. Ensembl Xenopus
Match: cep152 (centrosomal protein 152kDa [Source:Xenbase;Acc:XB-GENE-988609]) HSP 1 Score: 126.716 bits (317), Expect = 7.730e-33 Identity = 90/310 (29.03%), Postives = 147/310 (47.42%), Query Frame = 1
Query: 136 HMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFE-------ALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSND---SVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEG-ISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
H S ++ +GAL+G + + PLD KI FQ+ S K++Y + R YL EG W+G+ I + A+QF E + Y G + T LL G +AG+TAT+ P+D++R R+ + + Y ++ + ++MS EEG+ +RG PTVL + P I F Y LK +++AEH L+ G+ AG+ ++ YPLD+ +RR+Q G H ++ ++++ V EEG I GL+KGL + +K V ++F ++
Sbjct: 38 HKSILNSLMSGALAGAVAKTAVAPLDRTKIIFQVSSNRFSA--KEAY-----RLIYRTYLNEGFLSLWRGNSATMVRVIPYAAIQFCAHEQYKKLLGSYYGFQGSALTPIPRLLAGALAGTTATIITYPLDLVRARMAVTPK-EMYSNIIHVFMRMSREEGLKSLYRGFTPTVLGVIPYAGISFFTYETLK----------------KLHAEHSGRTQPYPFERLLFGACAGLFGQSASYPLDVVRRRMQTAGVTG--------HAYGSIIGTMQEIVAEEGVIRGLYKGLSMNWVKGPVAVGISFTTFD 315 HSP 2 Score: 70.0922 bits (170), Expect = 8.841e-13 Identity = 57/204 (27.94%), Postives = 91/204 (44.61%), Query Frame = 1
Query: 445 NNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY--KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSN-DSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
N+L+ G +AG+ A P+D RT+++ Q ++ K I + EG +RG T++ + P AIQF + Q K L S Y S + L++G++AG A + YPLDL + R+ V E Y H +RM EEG+ L++G P+ + + ++FF YE K+
Sbjct: 43 NSLMSGALAGAVAKTAVAPLD--RTKIIFQVSSNRFSAKEAYRLIYRTYLNEGFLSLWRGNSATMVRVIPYAAIQFCAHEQYKKLLGSY------------YGFQGSALTPIPRLLAGALAGTTATIITYPLDLVRARMAVTPKE---MYSNIIHVFMRMSR-------EEGLKSLYRGFTPTVLGVIPYAGISFFTYETLKKL 222
BLAST of slc25a-20 vs. Ensembl Xenopus
Match: slc25a24 (solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24 [Source:NCBI gene;Acc:496457]) HSP 1 Score: 103.605 bits (257), Expect = 5.715e-24 Identity = 83/297 (27.95%), Postives = 135/297 (45.45%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFE---ALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALV-SGSVAGIVAKTVIYPLDLAKRRLQVQG-FEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKF 1038
AG ++G + R T PLD LK+ Q+ + NI K++ E GI W+G+ A++F +E L++ + + G +AG+TA P++VL+TRL + G+ +Y + + K+ EGV F++G +P +L I P I IY LK+ + ++ YA +N V L+ G+ + + YPL L + R+Q Q E A Q L M RK V +EG GL++G+ P+ +K S+++ YEK
Sbjct: 138 AGGMAGAVSRTGTAPLDRLKVMMQVHGSKGNA--------NIITGLKQMVKEGGIRSLWRGNGVNVIKIAPETAMKFWAYEQYKKLFTSESGKLGTAERFIAGSLAGATAQTSIYPMEVLKTRL-AVGKTGQYSGMFDCAKKIMQREGVRAFYKGYIPNILGIIPYAGIDLAIYETLKT------------FWLQNYATDSANPGVLVLLGCGTASSTCGQLASYPLALIRTRMQAQASIEGAPQ--------LNMGGLFRKIVAKEGFFGLYRGIAPNFLKVLPAVSISYVVYEKM 405 HSP 2 Score: 77.0258 bits (188), Expect = 6.410e-15 Identity = 55/195 (28.21%), Postives = 91/195 (46.67%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL--YSKTGYSRTSTNN----LLFGGVAGSTA-TLFCQPIDVLRTRLVSQGEYKKYKSLTNAIL--KMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSL 696
+ T F AG+L+G + P++VLK R + K Y + +K+I EG+ F+KG++P I + + +E L + Y+ S N LL G A ST L P+ ++RTR+ +Q + L L K+ A+EG +G +RG+ P L + P +I + +Y ++K L
Sbjct: 223 LGTAERFIAGSLAGATAQTSIYPMEVLKTRLAVG--------KTGQYSGMFDCAKKIMQREGVRAFYKGYIPNILGIIPYAGIDLAIYETLKTFWLQNYATDSANPGVLVLLGCGTASSTCGQLASYPLALIRTRMQAQASIEGAPQLNMGGLFRKIVAKEGFFGLYRGIAPNFLKVLPAVSISYVVYEKMKIKL 409 HSP 3 Score: 68.5514 bits (166), Expect = 4.087e-12 Identity = 50/178 (28.09%), Postives = 82/178 (46.07%), Query Frame = 1
Query: 451 LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPS 984
LL GG+AG+ + P+D L+ + G K ++ + +M E G+ +RG V+ I P+TA++F Y Q K +S E + ++GS+AG A+T IYP+++ K RL V QY G F + +K + EG+ +KG +P+
Sbjct: 136 LLAGGMAGAVSRTGTAPLDRLKVMMQVHGS-KGNANIITGLKQMVKEGGIRSLWRGNGVNVIKIAPETAMKFWAYEQYKKLFTS---------------ESGKLGTAERFIAGSLAGATAQTSIYPMEVLKTRLAVG---KTGQYSGMFDCA-------KKIMQREGVRAFYKGYIPN 287
BLAST of slc25a-20 vs. Ensembl Mouse
Match: Slc25a19 (solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 [Source:MGI Symbol;Acc:MGI:1914533]) HSP 1 Score: 233.417 bits (594), Expect = 5.117e-74 Identity = 131/310 (42.26%), Postives = 181/310 (58.39%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI**IYFFCWR 1077
AG++SGF+ R + PLDV+KIRFQLQ+E + ++ Y I QA+K+I EEG FWKGHVPAQ LSI +GAVQF FE LY Y + + + + GG++ TATL P+DVLRTRL +QGE K Y +L AI M EG + F++GL PTV+ IFP +QF Y LK + +++I + N + L+ G +G+++KT YPLDL K+RLQV GFE AR FG+ ++ ++ + EEG G FKGL PS +K+ + + FF+YE F + F C R
Sbjct: 21 AGSVSGFVTRALISPLDVIKIRFQLQIERLCPSDPNAKYHGIFQAAKQILQEEGPRAFWKGHVPAQILSIGYGAVQFLAFEELTELLYQANLYQTHQFSAHFVCGGLSAGTATLTVHPVDVLRTRLAAQGEPKIYNNLREAIRTMYKTEGPFVFYKGLTPTVIAIFPYAGLQFSCYRSLKRAY---------DWLIPPDGKQTGN--LKNLLCGCGSGVISKTFTYPLDLIKKRLQVGGFEHARSAFGQVRSYRGLLDLTQQVLQEEGTRGFFKGLSPSLMKAALSTGFMFFWYELFCNL-----FHCIR 314
BLAST of slc25a-20 vs. Ensembl Mouse
Match: Slc25a19 (solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 [Source:MGI Symbol;Acc:MGI:1914533]) HSP 1 Score: 233.417 bits (594), Expect = 5.117e-74 Identity = 131/310 (42.26%), Postives = 181/310 (58.39%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI**IYFFCWR 1077
AG++SGF+ R + PLDV+KIRFQLQ+E + ++ Y I QA+K+I EEG FWKGHVPAQ LSI +GAVQF FE LY Y + + + + GG++ TATL P+DVLRTRL +QGE K Y +L AI M EG + F++GL PTV+ IFP +QF Y LK + +++I + N + L+ G +G+++KT YPLDL K+RLQV GFE AR FG+ ++ ++ + EEG G FKGL PS +K+ + + FF+YE F + F C R
Sbjct: 21 AGSVSGFVTRALISPLDVIKIRFQLQIERLCPSDPNAKYHGIFQAAKQILQEEGPRAFWKGHVPAQILSIGYGAVQFLAFEELTELLYQANLYQTHQFSAHFVCGGLSAGTATLTVHPVDVLRTRLAAQGEPKIYNNLREAIRTMYKTEGPFVFYKGLTPTVIAIFPYAGLQFSCYRSLKRAY---------DWLIPPDGKQTGN--LKNLLCGCGSGVISKTFTYPLDLIKKRLQVGGFEHARSAFGQVRSYRGLLDLTQQVLQEEGTRGFFKGLSPSLMKAALSTGFMFFWYELFCNL-----FHCIR 314
BLAST of slc25a-20 vs. Ensembl Mouse
Match: Slc25a19 (solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 [Source:MGI Symbol;Acc:MGI:1914533]) HSP 1 Score: 148.673 bits (374), Expect = 2.069e-42 Identity = 87/226 (38.50%), Postives = 124/226 (54.87%), Query Frame = 1
Query: 382 AVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSK 1044
A QF FE L Y Y + + + + GG++ TATL P+DVLRTRL +QGE K Y +L AI M EG + F++GL PTV+ IFP +QF Y LK + +++I + N + L+ G +G+++KT YPLDL K+RLQV GFE AR FG+ ++ ++ + EEG G FKGL PS +K+ + + FF+YE F
Sbjct: 11 AYQFLAFEELTELLYQANLYQTHQFSAHFVCGGLSAGTATLTVHPVDVLRTRLAAQGEPKIYNNLREAIRTMYKTEGPFVFYKGLTPTVIAIFPYAGLQFSCYRSLKRAY---------DWLIPPDGKQTGN--LKNLLCGCGSGVISKTFTYPLDLIKKRLQVGGFEHARSAFGQVRSYRGLLDLTQQVLQEEGTRGFFKGLSPSLMKAALSTGFMFFWYELFCN 225 HSP 2 Score: 80.8777 bits (198), Expect = 3.259e-17 Identity = 57/211 (27.01%), Postives = 92/211 (43.60%), Query Frame = 1
Query: 94 INSFIY*ITLFKVLHMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF-------RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---------VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYH 678
+ +Y L++ S + F G LS L P+DVL+ R Q EP Y N+++A + +Y EG F F+KG P + +QF R ++ L G + NLL G +G + F P+D+++ RL + G+ + Y+ L + ++ EEG GFF+GL P+++ T F Y
Sbjct: 20 LTELLYQANLYQTHQFSAH--FVCGGLSAGTATLTVHPVDVLRTRLAAQGEP-------KIYNNLREAIRTMYKTEGPFVFYKGLTPTVIAIFPYAGLQFSCYRSLKRAYDWLIPPDGKQTGNLKNLLCGCGSGVISKTFTYPLDLIKKRLQVGGFEHARSAFGQVRSYRGLLDLTQQVLQEEGTRGFFKGLSPSLMKAALSTGFMFFWYE 221
BLAST of slc25a-20 vs. Ensembl Mouse
Match: Slc25a19 (solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 [Source:MGI Symbol;Acc:MGI:1914533]) HSP 1 Score: 127.487 bits (319), Expect = 2.676e-34 Identity = 67/133 (50.38%), Postives = 87/133 (65.41%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYK 546
AG++SGF+ R + PLDV+KIRFQLQ+E + ++ Y I QA+K+I EEG FWKGHVPAQ LSI +GAVQF FE LY Y + + + + GG++ TATL P+DVLRTRL +QGE K
Sbjct: 21 AGSVSGFVTRALISPLDVIKIRFQLQIERLCPSDPNAKYHGIFQAAKQILQEEGPRAFWKGHVPAQILSIGYGAVQFLAFEELTELLYQANLYQTHQFSAHFVCGGLSAGTATLTVHPVDVLRTRLAAQGEPK 153
BLAST of slc25a-20 vs. Ensembl Mouse
Match: Slc25a16 (solute carrier family 25 (mitochondrial carrier, Graves disease autoantigen), member 16 [Source:MGI Symbol;Acc:MGI:1920382]) HSP 1 Score: 109.383 bits (272), Expect = 1.319e-26 Identity = 89/308 (28.90%), Postives = 152/308 (49.35%), Query Frame = 1
Query: 154 AFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQAS--KRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ--GEYKKYKSLTNAILKMSAEEGVY-GFFRGLVPTVLMIFPQTAIQFGIYHQLKS-----SLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYE 1032
+F AG ++G + PLD +K+ Q + +YK++ S + + +EG G +KG+ +GA+QF FE + +K G S + L+ G +AG TA + P+DV+R RL Q GE+ Y + +A + A+EG + GF+RGL+PT+L + P + F + LKS + + + S+ N + V H++ L+ G VAG +A+T+ YP D+ +RR+Q+ +F L M +++ + GI GL++GL + I+ ++ F YE
Sbjct: 39 SFLAGGIAGCCAKTTVAPLDRVKVLLQAH---------NRHYKHLGVLSTLRAVPQKEGYLGLYKGNGAMMIRIFPYGAIQFMAFEHYKTFITTKLGVS-GHVHRLMAGSMAGMTAVICTYPLDVVRVRLAFQVKGEHT-YSGIIHAFKTIYAKEGGFLGFYRGLMPTILGMAPYAGVSFFTFGTLKSVGLSYAPALLGRPSSDNPNVLVLKTHIN------LLCGGVAGAIAQTISYPFDVTRRRMQLGAV------LPEFEKCLTMRETMKYVYGQHGIRRGLYRGLSLNYIRCIPSQAVAFTTYE 323
BLAST of slc25a-20 vs. UniProt/SwissProt
Match: sp|Q29RM1|TPC_BOVIN (Mitochondrial thiamine pyrophosphate carrier OS=Bos taurus OX=9913 GN=SLC25A19 PE=2 SV=1) HSP 1 Score: 240.35 bits (612), Expect = 8.492e-76 Identity = 129/299 (43.14%), Postives = 181/299 (60.54%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALY-----SKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSK 1044
AG++SG + R++ PLDV+KIRFQLQ+E +S+ ++ Y I QA ++I EEG FWKGH+PAQ LSI +GAVQF +FEAL + +R + + L GG++ ATL P+DVLRTR +QGE + YK+L +A++ M EG F++GL PT++ IFP QF IY LK + Y + AE N + L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ + + EEG G FKGL PS +K+ + + L FF+YE F
Sbjct: 21 AGSVSGLVTRVLISPLDVIKIRFQLQIERLSRSDPNAKYHGILQAGRQILQEEGPTAFWKGHIPAQLLSIGYGAVQFLSFEALTELVHRASVRDARDFSVHFLCGGLSACVATLAVHPVDVLRTRFAAQGEPRVYKTLRDAVVTMYRTEGPLVFYKGLNPTLIAIFPYAGFQFSIYSSLKRA-----------YEWALPAEGKKNGNFKNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEQARASFGQVRSYKGLLDCAGQVLREEGAQGCFKGLSPSLLKAALSTGLVFFWYELFCN 308 HSP 2 Score: 78.1814 bits (191), Expect = 7.771e-15 Identity = 58/200 (29.00%), Postives = 82/200 (41.00%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF-------RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---------VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMR 708
F G LS + L P+DVL+ RF Q EP YK ++ A +Y EG F+KG P + QF R +E G + NLL G AG + P+D+ + RL S G+ + YK L + ++ EEG G F+GL P++L T + F Y + MR
Sbjct: 122 FLCGGLSACVATLAVHPVDVLRTRFAAQGEP-------RVYKTLRDAVVTMYRTEGPLVFYKGLNPTLIAIFPYAGFQFSIYSSLKRAYEWALPAEGKKNGNFKNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEQARASFGQVRSYKGLLDCAGQVLREEGAQGCFKGLSPSLLKAALSTGLVFFWYELFCNFFHHMR 314
BLAST of slc25a-20 vs. UniProt/SwissProt
Match: sp|Q9DAM5|TPC_MOUSE (Mitochondrial thiamine pyrophosphate carrier OS=Mus musculus OX=10090 GN=Slc25a19 PE=1 SV=1) HSP 1 Score: 233.417 bits (594), Expect = 3.579e-73 Identity = 131/310 (42.26%), Postives = 181/310 (58.39%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA----LYSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI**IYFFCWR 1077
AG++SGF+ R + PLDV+KIRFQLQ+E + ++ Y I QA+K+I EEG FWKGHVPAQ LSI +GAVQF FE LY Y + + + + GG++ TATL P+DVLRTRL +QGE K Y +L AI M EG + F++GL PTV+ IFP +QF Y LK + +++I + N + L+ G +G+++KT YPLDL K+RLQV GFE AR FG+ ++ ++ + EEG G FKGL PS +K+ + + FF+YE F + F C R
Sbjct: 21 AGSVSGFVTRALISPLDVIKIRFQLQIERLCPSDPNAKYHGIFQAAKQILQEEGPRAFWKGHVPAQILSIGYGAVQFLAFEELTELLYQANLYQTHQFSAHFVCGGLSAGTATLTVHPVDVLRTRLAAQGEPKIYNNLREAIRTMYKTEGPFVFYKGLTPTVIAIFPYAGLQFSCYRSLKRAY---------DWLIPPDGKQTGN--LKNLLCGCGSGVISKTFTYPLDLIKKRLQVGGFEHARSAFGQVRSYRGLLDLTQQVLQEEGTRGFFKGLSPSLMKAALSTGFMFFWYELFCNL-----FHCIR 314
BLAST of slc25a-20 vs. UniProt/SwissProt
Match: sp|Q5IS35|TPC_MACFA (Mitochondrial thiamine pyrophosphate carrier OS=Macaca fascicularis OX=9541 GN=SLC25A19 PE=2 SV=1) HSP 1 Score: 232.646 bits (592), Expect = 9.509e-73 Identity = 127/300 (42.33%), Postives = 182/300 (60.67%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG++SG + R + P DV+KIRFQLQ E +S+ ++ Y I QAS++I EEG+ FWKGH+PAQ LSI +GAVQF +FE L + + Y +R + + + GG+A TATL P+DVLRTR +QGE K Y +L +A+ M EG F++GL PT++ IFP +QF Y LK Y + AE N+++ L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ ++ + +EG G FKGL PS +K+ + + FF+YE F +
Sbjct: 21 AGSVSGLVTRALISPFDVIKIRFQLQHERLSRRDPNAKYHGIFQASRQILQEEGLTAFWKGHIPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACTATLTVHPVDVLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLK-----------HLYKWAMPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFWYEFFCNV 309 HSP 2 Score: 53.9138 bits (128), Expect = 9.785e-7 Identity = 50/215 (23.26%), Postives = 85/215 (39.53%), Query Frame = 1
Query: 424 GYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
G + T + G V+G P DV++ R Q E KY + A ++ EEG+ F++G +P ++ A+QF L + + + Y R ++ H V G +A A ++P+D+ + R QG K +++LR H++ EG +KGL P+ I + L F Y +
Sbjct: 10 GRNNTKLQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRRDPNAKYHGIFQASRQILQEEGLTAFWKGHIPAQILSIGYGAVQF-----LSFEMLTELVHRGSVYDAREFSVHF--------VCGGLAACTATLTVHPVDVLRTRFAAQGEP-------KVYNTLR--HAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHL 202
BLAST of slc25a-20 vs. UniProt/SwissProt
Match: sp|Q5NVC1|TPC_PONAB (Mitochondrial thiamine pyrophosphate carrier OS=Pongo abelii OX=9601 GN=SLC25A19 PE=2 SV=1) HSP 1 Score: 231.491 bits (589), Expect = 2.706e-72 Identity = 126/300 (42.00%), Postives = 180/300 (60.00%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG++SG + R + P DV+KIRFQLQ E +S+ ++ Y I QAS++I EEG FWKGH+PAQ LSI +GAVQF +FE L + + Y +R + + + GG+A ATL P+DVLRTR +QGE K Y +L +A+ M EG F++GL PT++ IFP +QF Y LK Y + AE N+++ L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ ++ + +EG G FKGL PS +K+ + + FF+YE F +
Sbjct: 21 AGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPNAKYHGILQASRQILQEEGPTAFWKGHIPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVDVLRTRFAAQGEPKVYNTLCHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLK-----------HLYKWAIPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFWYEFFCNV 309 HSP 2 Score: 51.2174 bits (121), Expect = 6.486e-6 Identity = 49/215 (22.79%), Postives = 84/215 (39.07%), Query Frame = 1
Query: 424 GYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
G + T + G V+G P DV++ R Q E KY + A ++ EEG F++G +P ++ A+QF L + + + Y R ++ H V G +A +A ++P+D+ + R QG K +++L H++ EG +KGL P+ I + L F Y +
Sbjct: 10 GRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPNAKYHGILQASRQILQEEGPTAFWKGHIPAQILSIGYGAVQF-----LSFEMLTELVHRGSVYDAREFSVHF--------VCGGLAACMATLTVHPVDVLRTRFAAQGEP-------KVYNTL--CHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHL 202
BLAST of slc25a-20 vs. UniProt/SwissProt
Match: sp|Q9HC21|TPC_HUMAN (Mitochondrial thiamine pyrophosphate carrier OS=Homo sapiens OX=9606 GN=SLC25A19 PE=1 SV=1) HSP 1 Score: 228.409 bits (581), Expect = 4.413e-71 Identity = 127/300 (42.33%), Postives = 178/300 (59.33%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
AG++SG + R + P DV+KIRFQLQ E +S+ + Y I QAS++I EEG FWKGHVPAQ LSI +GAVQF +FE L + + Y +R + + + GG+A ATL P+DVLRTR +QGE K Y +L +A+ M EG F++GL PT++ IFP +QF Y LK Y + AE N+++ L+ GS AG+++KT+ YPLDL K+RLQV GFE AR FG+ ++ ++ + +EG G FKGL PS +K+ + + FF YE F +
Sbjct: 21 AGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVDVLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLK-----------HLYKWAIPAEGKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALGFFKGLSPSLLKAALSTGFMFFSYEFFCNV 309 HSP 2 Score: 53.1434 bits (126), Expect = 1.391e-6 Identity = 51/215 (23.72%), Postives = 85/215 (39.53%), Query Frame = 1
Query: 424 GYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
G + T + G V+G P DV++ R Q E KY + A ++ EEG F++G VP ++ A+QF L + + + Y R ++ H V G +A +A ++P+D+ + R QG K +++LR H++ EG +KGL P+ I + L F Y +
Sbjct: 10 GRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQILQEEGPTAFWKGHVPAQILSIGYGAVQF-----LSFEMLTELVHRGSVYDAREFSVHF--------VCGGLAACMATLTVHPVDVLRTRFAAQGEP-------KVYNTLR--HAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHL 202
BLAST of slc25a-20 vs. TrEMBL
Match: A0A0H3YKB7 (Slc25a-20 OS=Schmidtea mediterranea OX=79327 GN=slc25a-20 PE=2 SV=1) HSP 1 Score: 619.387 bits (1596), Expect = 0.000e+0 Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI
Sbjct: 1 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 304
BLAST of slc25a-20 vs. TrEMBL
Match: A0A2G8JMF0 (Putative mitochondrial thiamine pyrophosphate carrier OS=Stichopus japonicus OX=307972 GN=BSL78_26224 PE=3 SV=1) HSP 1 Score: 262.692 bits (670), Expect = 4.325e-82 Identity = 143/300 (47.67%), Postives = 187/300 (62.33%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFE----ALYSKTGYSRTSTNNLL------FGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
AGA SGF RL+ QPLDVLKIRFQLQ EPISK S Y I+QA IY EEG+FGFWKGH PAQ LS+++G VQF FE +LY SR ++ L GG+AG A L P DVLRTR VSQGE K Y+S+ +A+ M E GV F+RGL+P ++ ++P T+ QF Y L + + S+ N S +V +L GS+AGI AKT++YPLDLAK+RL+VQGFEDAR++FG+ ++ +R +EG+ +KGL PS +K+ ++LTF FYE
Sbjct: 20 AGASSGFFTRLLLQPLDVLKIRFQLQSEPISKASPFSKYHGIRQAVSCIYREEGLFGFWKGHNPAQALSVIYGVVQFGCFEHLTASLYPHVP-SRFTSGPLKPIYHFGCGGIAGCAAALVAHPFDVLRTRFVSQGEPKIYRSIYHAVQSMYKEAGVRTFYRGLIPALVQVYPHTSFQFAFY-TLFNKMWESYFMSHSN----------SKGTVQSLTCGSLAGIAAKTIVYPLDLAKKRLEVQGFEDARRHFGRVEAYKNLIDCLRTMYQKEGVLSFYKGLKPSILKAAFSTALTFTFYE 307 HSP 2 Score: 73.9442 bits (180), Expect = 5.342e-11 Identity = 56/218 (25.69%), Postives = 97/218 (44.50%), Query Frame = 1
Query: 415 SKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGE-------YKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
S ++ T+T+ L G +G L QP+DVL+ R Q E + KY + A+ + EEG++GF++G P + +QFG + L +SL + ++ + G +AG A V +P D+ + R QG K + S+ H+++ E G+ ++GL+P+ ++ +S F FY F+KM
Sbjct: 6 SNVSHNFTTTDYQLAGASSGFFTRLLLQPLDVLKIRFQLQSEPISKASPFSKYHGIRQAVSCIYREEGLFGFWKGHNPAQALSVIYGVVQFGCFEHLTASLYPHVPSRFTSGPLK---------PIYHFGCGGIAGCAAALVAHPFDVLRTRFVSQGEP-------KIYRSI--YHAVQSMYKEAGVRTFYRGLIPALVQVYPHTSFQFAFYTLFNKM 205
BLAST of slc25a-20 vs. TrEMBL
Match: G7Y465 (Mitochondrial thiamine pyrophosphate carrier OS=Clonorchis sinensis OX=79923 GN=SLC25A19 PE=3 SV=1) HSP 1 Score: 263.077 bits (671), Expect = 6.341e-82 Identity = 143/327 (43.73%), Postives = 206/327 (63.00%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKT----GYSRTSTN-NLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSV---NALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFG---------------------KFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
F AG+LSGF VRL+TQP DVLKIRFQLQVEPI + SYY ++ QA RI+ EEGI+G WKGHVP Q LS+ F V+F F L + + GY +T + +L++G VAG+ A CQP+DV+RTRLV+QG+ + Y L +L++ EGV +RGL P+ ++I PQTA+ F Y QLK + + I S + V + L DS+ +L++GSV+G++AKT +YPLDL K+RL V+GFE+AR+ FG +F+ +L H I +++EG+ GLFKG PS+ K+ + + LTF F+E++ +++
Sbjct: 9 FLAGSLSGFSVRLLTQPFDVLKIRFQLQVEPIKRLSPASYYSSLPQAFCRIFREEGIYGLWKGHVPGQLLSVTFCGVEFAVFYGLKALSATSFGYLQTHVHRDLIYGTVAGTIAMTLCQPLDVMRTRLVAQGQKRVYSGLVMGLLELVRNEGVLALWRGLGPSCVLIVPQTAVTFAAYEQLKRTYQN-HIGSITRSSVNVSSPDL-KDSLPRWASLIAGSVSGLIAKTAVYPLDLIKKRLAVRGFEEARRCFGQVPDSYTAASYRLSNLRRVPTQFYATLACFHGI---LVQEGLIGLFKGWTPSACKAMLSTGLTFLFFEQYLQLL 330
BLAST of slc25a-20 vs. TrEMBL
Match: A0A4S2L938 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008880 PE=3 SV=1) HSP 1 Score: 262.692 bits (670), Expect = 7.299e-82 Identity = 143/324 (44.14%), Postives = 203/324 (62.65%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGYSRTSTN-NLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVN---ALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHS----------LRMVHS--------IRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
F AG+LSGF VRL+TQP DVLKIRFQLQVEPI + SYY ++ QA RI+ EEGI+G WKGHVP Q LS+ F V+F F L + GY + + +L++G AG+ AT FCQP+DV+RTRLV+QG+ + Y L +L++ EGV+ +RGL P+ ++I PQTA+ F Y QLK + + N++ V + DS+ +L +GSV+G+VAKT +YPLDL K+RL V+GFE+AR FG+ S LR V + R +++EG+ GLFKG PS+ K+ + + LTF F+E++ +++
Sbjct: 9 FLAGSLSGFSVRLLTQPFDVLKIRFQLQVEPIKRFSPASYYSSLPQAFCRIFREEGIYGLWKGHVPGQLLSVTFCGVEFAVFYGLKALPATSFGYLQAHVHRDLIYGTAAGTIATTFCQPLDVMRTRLVAQGQKRVYSGLVMGLLELVRNEGVFALWRGLGPSCMLIVPQTAVTFATYEQLKRTYEKHIMSINRSSVNASSPDL--KDSLPRWASLFAGSVSGLVAKTAVYPLDLIKKRLAVRGFEEARHCFGQVPDSYTAASYKLSTLRRVPTQLYATLACFRGILVQEGLIGLFKGWTPSAFKAMLSTGLTFLFFEQYLQLL 330
BLAST of slc25a-20 vs. TrEMBL
Match: W5N703 (Solute carrier family 25 member 19 OS=Lepisosteus oculatus OX=7918 PE=3 SV=1) HSP 1 Score: 258.07 bits (658), Expect = 2.890e-80 Identity = 135/306 (44.12%), Postives = 187/306 (61.11%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFS 1041
+ST AG+L+G + R + PLDV+KIRFQLQ+E +S Y + QA +RI EEG+ FWKGH+PAQ LSI FGAVQF +FE L + T Y SRT+ + + GG+A +AT+ CQP+D LRTR +QGE K Y +L +A+ M EGV F+RGL PT++ +FP +QF Y+ L+ Y+ +++ + D+V LV G AG+++KT+ YP DL K+RLQV+GFE+AR FG+ V + K EEG+ G FKGL PS IK+ + + TFF+YE F
Sbjct: 13 LSTEEVMLAGSLAGMVTRALISPLDVVKIRFQLQIEKVSARSPQGKYHGLWQACQRIRAEEGLRAFWKGHIPAQLLSIGFGAVQFASFEFLTEFVHKATPYNSRTAGVHFVCGGLAACSATVACQPLDTLRTRFAAQGEPKVYHNLRHALSTMYRTEGVLAFYRGLTPTMIAVFPYAGMQFFFYNILQ-----------HVYLWAMHSNASATDNVRNLVCGGCAGVISKTITYPFDLFKKRLQVRGFEEARIPFGQVRSYQGFVDCMAKIAQEEGLRGFFKGLSPSLIKAAMSTGFTFFWYELFC 307 HSP 2 Score: 85.5001 bits (210), Expect = 5.750e-15 Identity = 55/200 (27.50%), Postives = 87/200 (43.50%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF---RTFEALYSKTGYSRTSTN----NLLFGGVAGSTATLFCQPIDVLRTRL---------VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMR 708
F G L+ + QPLD L+ RF Q EP Y N++ A +Y EG+ F++G P + +QF + +Y +S S NL+ GG AG + P D+ + RL + G+ + Y+ + + K++ EEG+ GFF+GL P+++ T F Y SL +R
Sbjct: 122 FVCGGLAACSATVACQPLDTLRTRFAAQGEP-------KVYHNLRHALSTMYRTEGVLAFYRGLTPTMIAVFPYAGMQFFFYNILQHVYLWAMHSNASATDNVRNLVCGGCAGVISKTITYPFDLFKKRLQVRGFEEARIPFGQVRSYQGFVDCMAKIAQEEGLRGFFKGLSPSLIKAAMSTGFTFFWYELFCDSLRLLR 314
BLAST of slc25a-20 vs. Ensembl Cavefish
Match: slc25a19 (solute carrier family 25 member 19 [Source:NCBI gene;Acc:103042047]) HSP 1 Score: 162.925 bits (411), Expect = 2.715e-48 Identity = 83/201 (41.29%), Postives = 121/201 (60.20%), Query Frame = 1
Query: 448 NLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
+ L GG+A +AT+ CQP+D LRTR +QGE K Y++L +A++ M +EG F+RGL PT++ +FP +QF Y+ K L + +S + +L+SGS AG+++KT+ YP DL K+RLQV GFE AR +FG+ V + + EEG+ G FKGL PS +K+ V + LTFF+YE F+ I
Sbjct: 5 HFLCGGLAACSATVACQPLDTLRTRFAAQGEPKVYRTLRHAVVTMYRQEGALTFYRGLTPTLVAVFPYAGLQFFFYNIFKKLLDNDSTKSK--------------GGLQSLLSGSAAGVISKTLTYPFDLFKKRLQVGGFEQARMHFGQVRTYRGFVDCAMRILKEEGLRGFFKGLSPSLLKAAVSTGLTFFWYEFFTSAI 191 HSP 2 Score: 77.411 bits (189), Expect = 2.574e-16 Identity = 50/197 (25.38%), Postives = 90/197 (45.69%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTF----EALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---------VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMR 708
F G L+ + QPLD L+ RF Q EP Y+ ++ A +Y +EG F++G P + +QF + + L + + S+ +LL G AG + P D+ + RL + G+ + Y+ + +++ EEG+ GFF+GL P++L T + F Y S++++++
Sbjct: 6 FLCGGLAACSATVACQPLDTLRTRFAAQGEP-------KVYRTLRHAVVTMYRQEGALTFYRGLTPTLVAVFPYAGLQFFFYNIFKKLLDNDSTKSKGGLQSLLSGSAAGVISKTLTYPFDLFKKRLQVGGFEQARMHFGQVRTYRGFVDCAMRILKEEGLRGFFKGLSPSLLKAAVSTGLTFFWYEFFTSAIANLK 195
BLAST of slc25a-20 vs. Ensembl Cavefish
Match: slc25a42 (solute carrier family 25 member 42 [Source:NCBI gene;Acc:103032404]) HSP 1 Score: 112.849 bits (281), Expect = 5.232e-28 Identity = 83/305 (27.21%), Postives = 142/305 (46.56%), Query Frame = 1
Query: 142 STNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF-------RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEG-ISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
S ++ +GAL+G + + PLD KI FQ+ S K++Y + R Y ++G W+G+ I + A+QF R Y G + LL G +AG+TA P+D++R R+ + + Y ++ + +++S EEG+ +RG PT+L + P + F Y LK L + + Q Y S L G+ AG++ ++ YPLD+ +RR+Q G H ++ ++R+ V EEG I GL+KGL + +K + ++F ++
Sbjct: 34 SVLNSLLSGALAGAVAKTAVAPLDRTKIIFQVSSNRFSA--KEAY-----RLIYRTYRKDGFLSLWRGNSATMVRVIPYAAIQFCAHEQYKRLLGGYYGFQGKALPPVPRLLAGSLAGTTAASLTYPLDMVRARMAVTPK-EMYSNILHVFVRISREEGLKTLYRGFTPTILGVVPYAGLSFFTYETLK-KLHAEKTGRPQPY------------SFERLAFGACAGLIGQSSSYPLDVVRRRMQTAGVTG--------HTYGTILGTMREIVAEEGVIRGLYKGLSMNWVKGPIAVGISFMTFD 309 HSP 2 Score: 68.9366 bits (167), Expect = 1.133e-12 Identity = 57/208 (27.40%), Postives = 95/208 (45.67%), Query Frame = 1
Query: 433 RTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY--KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYA-EHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
R+ N+LL G +AG+ A P+D RT+++ Q ++ K I + ++G +RG T++ + P AIQF + Q K L Y + + V L++GS+AG A ++ YPLD+ + R+ V E Y H +R+ EEG+ L++G P+ + + L+FF YE K+
Sbjct: 33 RSVLNSLLSGALAGAVAKTAVAPLD--RTKIIFQVSSNRFSAKEAYRLIYRTYRKDGFLSLWRGNSATMVRVIPYAAIQFCAHEQYKRLLGGY------------YGFQGKALPPVPRLLAGSLAGTTAASLTYPLDMVRARMAVTPKE---MYSNILHVFVRISR-------EEGLKTLYRGFTPTILGVVPYAGLSFFTYETLKKL 216
BLAST of slc25a-20 vs. Ensembl Cavefish
Match: slc25a24 (solute carrier family 25 member 24 [Source:NCBI gene;Acc:103040313]) HSP 1 Score: 110.923 bits (276), Expect = 1.389e-26 Identity = 90/299 (30.10%), Postives = 138/299 (46.15%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFE---ALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLV--SQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALV-SGSVAGIVAKTVIYPLDLAKRRLQVQG-FEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
AGA++G + R T PLD +K+ Q+ SK K S KQ K E G+ W+G+ A++F +E L SK G + + G +AG+TA P++V++TRL G+Y ILK +EGV F++G VP +L I P I IY LK N + +YA+ +N V L+ G+++ + YPL L + R+Q Q E A Q + M +RK + +EG GL++G+LP+ +K S+++ YE
Sbjct: 198 LAAGAMAGAVSRTGTAPLDRMKVFMQVHS---SKSNKISLVSGFKQMIK----EGGVASLWRGNGVNVIKIAPETAIKFMAYEQYKKLLSKEGGKIRTHERFMAGSLAGATAQTAIYPMEVMKTRLTLRKTGQYSGMFDCAKKILK---KEGVKAFYKGYVPNILGIIPYAGIDLAIYETLK------------NTWLSLYAKDTANPGVLVLLGCGTISSTCGQLASYPLALVRTRMQAQASMEGAEQ--------VSMSKLVRKILEKEGFFGLYRGILPNFMKVIPAVSISYVVYE 466 HSP 2 Score: 78.5666 bits (192), Expect = 1.633e-15 Identity = 49/193 (25.39%), Postives = 97/193 (50.26%), Query Frame = 1
Query: 145 TNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSK--TGYSRTSTNN-----LLFGGVAGSTATLFCQPIDVLRTRLVSQG--EYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSL 696
T+ F AG+L+G + P++V+K R L+ K Y + +K+I +EG+ F+KG+VP I + + +E L + + Y++ + N L G ++ + L P+ ++RTR+ +Q E + S++ + K+ +EG +G +RG++P + + P +I + +Y +K+ L
Sbjct: 288 THERFMAGSLAGATAQTAIYPMEVMKTRLTLR--------KTGQYSGMFDCAKKILKKEGVKAFYKGYVPNILGIIPYAGIDLAIYETLKNTWLSLYAKDTANPGVLVLLGCGTISSTCGQLASYPLALVRTRMQAQASMEGAEQVSMSKLVRKILEKEGFFGLYRGILPNFMKVIPAVSISYVVYEYMKTGL 472
BLAST of slc25a-20 vs. Ensembl Cavefish
Match: slc25a23a (calcium-binding mitochondrial carrier protein SCaMC-3 [Source:NCBI gene;Acc:103026535]) HSP 1 Score: 108.612 bits (270), Expect = 1.072e-25 Identity = 88/304 (28.95%), Postives = 141/304 (46.38%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALY-----SKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSS-LSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
AGA++G + R T PLD LK+ LQV S D K S +K +K K E GI W+G+ A++F +E + SK G S + G +AG+TA P++VL+TRL + + +Y S+ + ++ +EGV F++G +P +L I P I +Y LK++ L R S V+ + G+V+ + YPL L + R+Q Q K L M+ R V +EG+ GL++G+ P+ +K S+++ YE K++
Sbjct: 188 LMAGAMAGSVSRTGTAPLDRLKVF--LQVHGQSSD-KGSVWKGLKAMVK----EGGIRALWRGNGINVLKIAPETAIKFLAYEQIKRMMNGSKDGGSLKVQERFIAGSLAGATAQTIIYPMEVLKTRLTLR-KTGQYSSVVDCARQILQKEGVLAFYKGYIPNILGIIPYAGIDLAVYETLKNTWLQRHRCDSADPGVLVLVG------------CGTVSSTCGQLASYPLALIRTRMQAQASV-------KGGPQLSMLSLFRSIVAQEGVVGLYRGIAPNFLKVIPAVSISYVVYEHMRKVL 464
BLAST of slc25a-20 vs. Ensembl Cavefish
Match: slc25a32a (solute carrier family 25 member 32a [Source:ZFIN;Acc:ZDB-GENE-040426-758]) HSP 1 Score: 105.145 bits (261), Expect = 2.198e-25 Identity = 88/324 (27.16%), Postives = 147/324 (45.37%), Query Frame = 1
Query: 115 ITLFKVLHMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQ----LQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTST----NNLLFGGVAGSTATLFCQPIDVLRTRLV----SQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
++L K + + AG G I ++ PLD++KIRF L+V P Y+ + + I+ EGI G ++G P + + + F + A+ S T R S +L+ AG P+ V +TRLV ++ K+YK + +A++K+ EG+ G +RG +P L Q A+QF Y LK QN ++++E L + + +V +V+ I A YP + + RLQ Q Y G + IR+T EG+ G +KG++P+ ++ +TF YE S+ +
Sbjct: 5 LSLLKYIRLEN---LAAGLSGGVIATMVLHPLDLIKIRFAVSDGLKVRP--------QYEGMMHCMRSIWQVEGIRGLYQGVTPNIWGAGASWGLYFLFYNAIKSYTQEGRQSELSALEHLVSAAEAGILTLCLTNPVWVTKTRLVLQYNAEPNGKQYKGMLDALVKIYRHEGIPGLYRGFLPG-LFGTTQAALQFMTYEALKRE---------QNKYKKMHSEALLS-PLEYIVMAAVSKIAAVAATYPYQVVRARLQDQ----HNNYSG-------IADVIRRTWSNEGVEGFYKGMVPNLVRVIPACCITFLVYENVSRFL 295
BLAST of slc25a-20 vs. Ensembl Sea Lamprey
Match: slc25a23a (solute carrier family 25 member 23a [Source:ZFIN;Acc:ZDB-GENE-121214-154]) HSP 1 Score: 97.0561 bits (240), Expect = 2.393e-22 Identity = 81/301 (26.91%), Postives = 135/301 (44.85%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTST---NNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVS-GSVAGIVAKTVIYPLDLAKRRLQVQG-FEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
AG +G + R T PLD +K+ Q+ SK S KQ K E GI W+G+ A++F +E + G + S + G +AG+TA P++V++TRL + + +Y + + K+ +EGV F++G VP ++ I P I IY LK N + YA+ ++ + L++ G+V+ + YPL L + R+Q Q E Q L MV R V +G GL++G+ P+ +K S+++ YE +++
Sbjct: 216 AGGAAGAVSRTGTAPLDRMKVFMQVHG---SKSNNISILTGFKQMIK----EGGIASLWRGNGVNVIKIAPETAIKFMAYEQIKKFIGSNNVSLGIHERFMAGSLAGATAQTIIYPMEVMKTRLTLR-KTGQYSGMMDCAKKIFMKEGVIAFYKGYVPNIIGIIPYAGIDLAIYETLK------------NTWLTKYAKDTADPGILVLLACGTVSSSCGQVASYPLALIRTRMQAQASMEGGPQ--------LSMVGLFRNIVKNDGPLGLYRGIAPNFMKVIPAVSISYVVYENLKRVL 488 HSP 2 Score: 80.1073 bits (196), Expect = 1.027e-16 Identity = 49/189 (25.93%), Postives = 91/189 (48.15%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSK--TGYSRTSTNN-----LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAIL--KMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSL 696
F AG+L+G + I P++V+K R L+ K Y + +K+I+++EG+ F+KG+VP I + + +E L + T Y++ + + L G V+ S + P+ ++RTR+ +Q + L+ L + +G G +RG+ P + + P +I + +Y LK L
Sbjct: 308 FMAGSLAGATAQTIIYPMEVMKTRLTLR--------KTGQYSGMMDCAKKIFMKEGVIAFYKGYVPNIIGIIPYAGIDLAIYETLKNTWLTKYAKDTADPGILVLLACGTVSSSCGQVASYPLALIRTRMQAQASMEGGPQLSMVGLFRNIVKNDGPLGLYRGIAPNFMKVIPAVSISYVVYENLKRVL 488 HSP 3 Score: 71.2478 bits (173), Expect = 8.674e-14 Identity = 49/180 (27.22%), Postives = 86/180 (47.78%), Query Frame = 1
Query: 451 LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSI 990
L+ GG AG+ + P+D ++ + G S+ +M E G+ +RG V+ I P+TAI+F Y Q+K + S N + ++ ++GS+AG A+T+IYP+++ K RL ++ QY G M+ +K ++EG+ +KG +P+ I
Sbjct: 214 LMAGGAAGAVSRTGTAPLDRMKVFMQVHGSKSNNISILTGFKQMIKEGGIASLWRGNGVNVIKIAPETAIKFMAYEQIKKFIGS------NNVSLGIHER---------FMAGSLAGATAQTIIYPMEVMKTRLTLR---KTGQYSG-------MMDCAKKIFMKEGVIAFYKGYVPNII 368
BLAST of slc25a-20 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004577.1 (pep scaffold:Pmarinus_7.0:GL479453:699:15637:1 gene:ENSPMAG00000004140.1 transcript:ENSPMAT00000004577.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 93.9745 bits (232), Expect = 4.664e-22 Identity = 74/300 (24.67%), Postives = 130/300 (43.33%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYL--EEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTN---NLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALV-SGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKF 1038
AG +G + R T PLD LK+ Q+ K S + I ++ E G+ W+G+ A++F +E + G + L+ G +AG+TA P++V++TRL + G+ +Y S+ + K++ EG+ FF+G +P VL I P I Y LK N+ ++ + +N + L+ + + + YPL L + R+Q A G MV +R V ++G+ GL++G+ P+ +K +++ YE
Sbjct: 28 LVAGGGAGVVSRTCTAPLDRLKVIMQV---------KASRHNTIGVVGGLAHMIREGGVRSLWRGNGVNVIKIAPETAIKFMAYEQIKKLIGMRQEQIGVQERLVAGSLAGATAQTVIYPMEVVKTRL-ALGKTGQYSSMVDCARKIAKREGLRAFFKGYLPNVLGIIPYAGIDLATYETLK------------NWWLQKHGAEGANPGIAVLLCCATTSSTCGQLASYPLALVRTRMQA-----AASIGGPGASQPGMVGLMRSIVQQDGLLGLYRGIAPNFMKVIPAVGISYVVYENL 300 HSP 2 Score: 72.0182 bits (175), Expect = 1.859e-14 Identity = 58/185 (31.35%), Postives = 80/185 (43.24%), Query Frame = 1
Query: 430 SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPS 984
S T L+ GG AG + P+D L+ + + + + M E GV +RG V+ I P+TAI+F Y Q+K L MR E + LV+GS+AG A+TVIYP+++ K RL GK MV RK EG+ FKG LP+
Sbjct: 21 SGTWWRQLVAGGGAGVVSRTCTAPLDRLKVIMQVKASRHNTIGVVGGLAHMIREGGVRSLWRGNGVNVIKIAPETAIKFMAYEQIK-KLIGMR------------QEQIGVQ--ERLVAGSLAGATAQTVIYPMEVVKTRLA----------LGKTGQYSSMVDCARKIAKREGLRAFFKGYLPN 180
BLAST of slc25a-20 vs. Ensembl Sea Lamprey
Match: slc25a16 (solute carrier family 25 member 16 [Source:ZFIN;Acc:ZDB-GENE-030131-3299]) HSP 1 Score: 93.2041 bits (230), Expect = 1.310e-21 Identity = 90/322 (27.95%), Postives = 149/322 (46.27%), Query Frame = 1
Query: 145 TNSA-----FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYL--EEGIFGFWKGHVPAQYLSIVFGAVQFRTFEA---LYSKTGYSRTSTNNLLFGG--------VAGSTATLFCQPIDVLRTRLVSQ--GEYKKYKSLTNAI-LKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLS----NDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYE 1032
TNS+ F AG ++G + PLD +KI Q + +YK++ S + + +EG G +KG+ +GA+QF FE + SK + LL G G TA +F P+D++R RL Q G++ Y+ + +A L E G GF+ GLVPT++ + P F + S+L S+ + + R A++ +N L G+ AG +A+T+ YPLD+A+RR+Q+ G + FG +L V+ + G+ GL++GL + ++ ++ F YE
Sbjct: 30 TNSSRWMRNFLAGGIAGCCAKTTIAPLDRVKILLQAY---------NPHYKHLGVFSTLVAIPRKEGFLGLYKGNGAMMVRIFPYGAIQFTAFEKYKRVLSKQMGITGHVHRLLAGSHGWYLSPCAPGITAVIFTYPLDMVRARLAYQVKGDHG-YRGILHAFSLIYKKEGGFRGFYHGLVPTIIGMAPYAGFSFFTF----STLKSVGLTRAPALLGRPSADNPDVLVLRTPINLLFGGT-AGAIAQTISYPLDVARRRMQLAGILPNSENFGSMWQTLTNVYK------QYGVRRGLYRGLSLNYVRCVPSQAVAFTTYE 330
BLAST of slc25a-20 vs. Ensembl Sea Lamprey
Match: slc25a4 (solute carrier family 25 member 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5275]) HSP 1 Score: 88.1965 bits (217), Expect = 4.233e-20 Identity = 83/311 (26.69%), Postives = 140/311 (45.02%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFK-DSYYKNIKQASKRIYLEEGIFGFWKGHV-------PAQYLSIVFGAVQFRTFEALYSK-TGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRL---VSQGEY-KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
F AG ++ I + P++ +++ LQV+ SK D YK I RI E+G FW+G++ P Q L+ F + F K + R NL GG AG+T+ F P+D RTRL V +G+ +++K L + I K+ +G+ G ++G +V I A FGIY K L + + S V + A+ ++ +V+GIV+ YP D +RR+ +Q R+ + + +K + +EG FKG + I+ G + Y++ +++
Sbjct: 14 FMAGGIAAAISKTAVAPIE--RVKLLLQVQHASKQIAVDQQYKGIMDCVVRIPREQGFVSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGQAEREFKGLADCISKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPNPK--STPLVVSWMIAQSVT----------AVSGIVS----YPFDTVRRRMMMQ---SGRKGADIMYRG--TIDCWKKILQQEGGKAFFKGAWSNVIR-GAGGAFVLVLYDEIKRVL 300 HSP 2 Score: 56.6102 bits (135), Expect = 2.107e-9 Identity = 45/187 (24.06%), Postives = 85/187 (45.45%), Query Frame = 1
Query: 445 NNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY--------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRL--QVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGL 975
+ + GG+A + + PI+ R +L+ Q ++ ++YK + + ++++ E+G F+RG + V+ FP A+ F + K + Q + R +A +L+ SG AG + +YPLD A+ RL V + R++ G + I K +G+ GL++G
Sbjct: 12 KDFMAGGIAAAISKTAVAPIE--RVKLLLQVQHASKQIAVDQQYKGIMDCVVRIPREQGFVSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKHKQFW--RYFAGNLA--------SGGAAGATSLCFVYPLDFARTRLAADVGKGQAEREFKG-------LADCISKIFKSDGLRGLYQGF 179
BLAST of slc25a-20 vs. Ensembl Sea Lamprey
Match: ucp2 (uncoupling protein 2 [Source:NCBI gene;Acc:555812]) HSP 1 Score: 85.5001 bits (210), Expect = 5.225e-19 Identity = 56/193 (29.02%), Postives = 86/193 (44.56%), Query Frame = 1
Query: 136 HMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNL------LFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSL 696
H AG +G + QP DV+K+RFQ QV + + Y A K I EEG+ G WKG P + + + T++ + + T+NL FG AG T+ P+DV++TR ++ +Y+S N M +EG F++G VP+ L + + F Y QLK +
Sbjct: 115 HAGIGCRLLAGCTTGAMAVTFAQPTDVVKVRFQAQVNMLGTSKR---YSGTMNAYKTIAREEGVRGLWKGTGPNITRNAIVNCAELVTYDIIKDTILKYKLLTDNLPCHFVSAFG--AGFCTTVVASPVDVVKTRYMNSAP-GQYRSAFNCAYLMLTKEGAMAFYKGFVPSFLRLGSWNVVMFVTYEQLKRGI 301 HSP 2 Score: 72.7886 bits (177), Expect = 1.002e-14 Identity = 72/309 (23.30%), Postives = 121/309 (39.16%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKD----FKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL---YSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ----GEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
F + I LIT PLD K+R Q+Q E + Y+ + + EG + G V + F +V+ ++++ Y+ LL G G+ A F QP DV++ R +Q G K+Y NA ++ EEGV G ++G P + + Y +K ++ ++ L+++ VS AG V P+D+ K R QY F+ + M+ +EG +KG +PS ++ G + + F YE+ + I
Sbjct: 17 FIGAGTAACIADLITFPLDTAKVRLQVQGECQRGEGAARSAGVQYRGVFGTIAAMVRTEGPRSLYSGLVAGLQRQMSFASVRIGLYDSVKNFYTNGAEHAGIGCRLLAGCTTGAMAVTFAQPTDVVKVRFQAQVNMLGTSKRYSGTMNAYKTIAREEGVRGLWKGTGPNITRNAIVNCAELVTYDIIKDTILKYKL--------------LTDNLPCHFVSAFGAGFCTTVVASPVDVVKTRYMNSA---PGQYRSAFNCAYLMLT-------KEGAMAFYKGFVPSFLRLGSWNVVMFVTYEQLKRGI 301 HSP 3 Score: 53.9138 bits (128), Expect = 1.929e-8 Identity = 49/212 (23.11%), Postives = 86/212 (40.57%), Query Frame = 1
Query: 436 TSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKK-----------YKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQ--GFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
T+ + G A A L P+D + RL QGE ++ Y+ + I M EG + GLV + +++ G+Y +K+ ++ AEH L++G G +A T P D+ K R Q Q +++Y G +++ + EEG+ GL+KG P+ ++ +V+ Y+
Sbjct: 12 TAAVKFIGAGTAACIADLITFPLDTAKVRLQVQGECQRGEGAARSAGVQYRGVFGTIAAMVRTEGPRSLYSGLVAGLQRQMSFASVRIGLYDSVKNFYTNG-------------AEHAGIGC--RLLAGCTTGAMAVTFAQPTDVVKVRFQAQVNMLGTSKRYSGT-------MNAYKTIAREEGVRGLWKGTGPNITRNAIVNCAELVTYD 201
BLAST of slc25a-20 vs. Ensembl Yeast
Match: TPC1 (Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family [Source:SGD;Acc:S000003328]) HSP 1 Score: 122.865 bits (307), Expect = 9.029e-33 Identity = 86/301 (28.57%), Postives = 158/301 (52.49%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSK--TGYS-RTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQ------VQGFEDARQYFGKF--HHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
AGA+SG + R IT P+D +KIR QL K F + + ++ + EGI FWKG++P L + +G+ QF ++ +L+++ T + ++L+ G AG T+++ P DVLRTRLV+ + S+T + + EG+ GFF+G + ++ I +I FG Y ++RI ++N + ++N +G++ G++AK + +PL+ +RR+Q ++ F +G + + R+ I K +EG+S L++G+L + K+ + ++F+ YE
Sbjct: 22 AGAVSGLLARSITAPMDTIKIRLQLTPANGLKPFGS----QVMEVARSMIKNEGIRSFWKGNIPGSLLYVTYGSAQFSSY-SLFNRYLTPFGLEARLHSLVVGAFAGITSSIVSYPFDVLRTRLVANNQMHSM-SITREVRDIWKLEGLPGFFKGSIASMTTITLTASIMFGTYE-------TIRIYCDENEKTTAAHKKWELATLNH-SAGTIGGVIAKIITFPLETIRRRMQFMNSKHLEKFSRHSSVYGSYKGYGFARIGLQILK---QEGVSSLYRGILVALSKTIPTTFVSFWGYE 305
BLAST of slc25a-20 vs. Ensembl Yeast
Match: LEU5 (Mitochondrial carrier protein; involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein SLC25A16, which complements yeast null mutant; does not encode an isozyme of Leu4p, as first hypothesized [Source:SGD;Acc:S000001044]) HSP 1 Score: 114.39 bits (285), Expect = 2.708e-29 Identity = 92/327 (28.13%), Postives = 144/327 (44.04%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSR---TSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEG----------------VYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSS-----------------MRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEK 1035
AG +SG + + PLD +KI FQ P + S + +A+K I++ +G+ GF++GH + AV+F +E + + S+ + L+ G +AG + P+D++R RL + E+K+ K L I K+ E F+RG VPTVL + P + F + L L S R+Q Q +R +AE L+SG +AG+ ++T YP ++ +RRLQV Y KF + H I K E G+ G F GL IK + + +FF YE+
Sbjct: 39 AGGISGSCAKTLIAPLDRIKILFQTS-NPHYTKYTGSLI-GLVEAAKHIWINDGVRGFFQGHSATLLRIFPYAAVKFVAYEQIRNTLIPSKEFESHWRRLVSGSLAGLCSVFITYPLDLVRVRLAYETEHKRVK-LGRIIKKIYKEPASATLIKNDYIPNWFCHWCNFYRGYVPTVLGMIPYAGVSFFAHDLLHDVLKSPFFAPYSVLELSEDDELERVQKKQRRPLRTWAE---------LISGGLAGMASQTAAYPFEIIRRRLQVSALSPKTMYDHKFQSISEIAHIIFK---ERGVRGFFVGLSIGYIKVTPMVACSFFVYER 350 HSP 2 Score: 69.707 bits (169), Expect = 1.030e-13 Identity = 51/157 (32.48%), Postives = 77/157 (49.04%), Query Frame = 1
Query: 418 KTGYSRTSTNNL----LFGGVAGSTATLFCQPIDVLRTRL-VSQGEYKKYK----SLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQ 861
KT + R S + + L GG++GS A P+D ++ S Y KY L A + +GV GFF+G T+L IFP A++F Y Q++++L I S + + H LVSGS+AG+ + + YPLDL + RL +
Sbjct: 22 KTSFDRNSFDYIVRSGLAGGISGSCAKTLIAPLDRIKILFQTSNPHYTKYTGSLIGLVEAAKHIWINDGVRGFFQGHSATLLRIFPYAAVKFVAYEQIRNTL----IPSKE------FESHWRR-----LVSGSLAGLCSVFITYPLDLVRVRLAYE 163
BLAST of slc25a-20 vs. Ensembl Yeast
Match: AAC3 (Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication [Source:SGD;Acc:S000000289]) HSP 1 Score: 105.531 bits (262), Expect = 2.136e-26 Identity = 83/301 (27.57%), Postives = 131/301 (43.52%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHV-------PAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVS------QGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKG----LLPSSIKSGVVS 1008
F G +S I + P++ +KI Q Q E I + D Y I KR +EG+ FW+G+ P Q L+ F + + + GY + NL GG AG+ + LF +D RTRL + +G +++ LT+ K +G+ G +RG +P+V+ I + FG++ LK V L + + + G V A T YPLD +RR+ + + A +Y G + ++K V EG+ LFKG +L S +GV+S
Sbjct: 16 FLMGGVSAAIAKTAASPIERVKILIQNQDEMIKQGTLDKKYSGIVDCFKRTAKQEGLISFWRGNTANVIRYFPTQALNFAF-KDKIKLMFGFKKEEGYGKWFAGNLASGGAAGALSLLFVYSLDFARTRLAADAKSSKKGGARQFNGLTDVYKKTLKSDGIAGLYRGFMPSVVGIVVYRGLYFGMFDSLKP---------------LVLTGSLDGSFLASFLLGWVVTTGASTCSYPLDTVRRRMMMTSGQ-AVKYNGA-------IDCLKKIVASEGVGSLFKGCGANILRSVAGAGVIS 292 HSP 2 Score: 70.8626 bits (172), Expect = 3.319e-14 Identity = 52/212 (24.53%), Postives = 93/212 (43.87%), Query Frame = 1
Query: 448 NLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFED----ARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
N L GGV+ + A PI+ ++ + +Q E KKY + + + + +EG+ F+RG V+ FP A+ F ++K + + Y + + + L SG AG ++ +Y LD A+ RL ARQ+ G + +KT+ +GI+GL++G +PS + V L F ++ ++
Sbjct: 15 NFLMGGVSAAIAKTAASPIERVKILIQNQDEMIKQGTLDKKYSGIVDCFKRTAKQEGLISFWRGNTANVIRYFPTQALNFAFKDKIKLMFGFKKEEG--------YGKWFAGN----LASGGAAGALSLLFVYSLDFARTRLAADAKSSKKGGARQFNG-------LTDVYKKTLKSDGIAGLYRGFMPSVVGIVVYRGLYFGMFDSLKPLV 207
BLAST of slc25a-20 vs. Ensembl Yeast
Match: PET9 (Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication; human homolog SLC25A4 implicated in progressive external ophthalmoplegia can complement yeast null mutant [Source:SGD;Acc:S000000126]) HSP 1 Score: 105.531 bits (262), Expect = 2.907e-26 Identity = 82/311 (26.37%), Postives = 135/311 (43.41%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHV-------PAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVS------QGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
F G +S + + P++ +K+ Q Q E + + D Y I KR +EG+ FW+G+ P Q L+ F + + + GY++ NL GG AG+ + LF +D RTRL + +G +++ L + K +GV G +RG +P+V+ I + FG+Y LK L + L + + + G V A T YPLD +RR+ + + A +Y G F +RK V EG+ LFKG ++I GV + Y++ ++
Sbjct: 27 FLMGGVSAAVAKTAASPIERVKLLIQNQDEMLKQGTLDRKYAGILDCFKRTATQEGVISFWRGNTANVIRYFPTQALNFAF-KDKIKAMFGFKKEEGYAKWFAGNLASGGAAGALSLLFVYSLDYARTRLAADSKSSKKGGARQFNGLIDVYKKTLKSDGVAGLYRGFLPSVVGIVVYRGLYFGMYDSLKPLLLT---------------GSLEGSFLASFLLGWVVTTGASTCSYPLDTVRRRMMMTSGQ-AVKYDGAF-------DCLRKIVAAEGVGSLFKG-CGANILRGVAGAGVISMYDQLQMIL 312 HSP 2 Score: 73.1738 bits (178), Expect = 4.954e-15 Identity = 53/212 (25.00%), Postives = 96/212 (45.28%), Query Frame = 1
Query: 448 NLLFGGVAGSTATLFCQPIDVLRTRLVSQGEY-------KKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFED----ARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
+ L GGV+ + A PI+ ++ + +Q E +KY + + + + +EGV F+RG V+ FP A+ F ++K+ + + YA+ + + L SG AG ++ +Y LD A+ RL ARQ+ G ++ +KT+ +G++GL++G LPS + V L F Y+ ++
Sbjct: 26 DFLMGGVSAAVAKTAASPIERVKLLIQNQDEMLKQGTLDRKYAGILDCFKRTATQEGVISFWRGNTANVIRYFPTQALNFAFKDKIKAMFGFKKEEG--------YAKWFAGN----LASGGAAGALSLLFVYSLDYARTRLAADSKSSKKGGARQFNG-------LIDVYKKTLKSDGVAGLYRGFLPSVVGIVVYRGLYFGMYDSLKPLL 218
BLAST of slc25a-20 vs. Ensembl Yeast
Match: YPR011C_mRNA (Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies [Source:SGD;Acc:S000006215]) HSP 1 Score: 104.375 bits (259), Expect = 8.553e-26 Identity = 87/321 (27.10%), Postives = 151/321 (47.04%), Query Frame = 1
Query: 133 LHMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSK-------TGYSR-TSTNNLLFGGVAGSTATLFCQPIDVLRTRL------VSQGEYKKYKSLTN--AILKMSAEE-----GVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
L +N AF AG ++G + R + P + +KI Q+Q SY + I + +++Y EEG G ++G+ + AVQF +EA K G + T+T L G + G + + P+D+++TRL +S K KS++ I ++ +E G+ G +RG+ PT L + P A+ F +Y QL+ + S+ A+ ++ L G+++G VA+T+ YP DL +RR QV F ++ + +I + EG+SG +KGL + K +++++ YE
Sbjct: 18 LKQDSNIAFLAGGVAGAVSRTVVSPFERVKILLQVQSS------TTSYNRGIFSSIRQVYHEEGTKGLFRGNGLNCIRIFPYSAVQFVVYEACKKKLFHVNGNNGQEQLTNTQRLFSGALCGGCSVVATYPLDLIKTRLSIQTANLSSLNRSKAKSISKPPGIWQLLSETYRLEGGLRGLYRGVWPTSLGVVPYVALNFAVYEQLRE----FGVNSSD-------AQPSWKSNLYKLTIGAISGGVAQTITYPFDLLRRRFQVLAMGGNELGF-RYTSVWDALVTIGRA---EGVSGYYKGLAANLFKVVPSTAVSWLVYE 317 HSP 2 Score: 72.7886 bits (177), Expect = 7.697e-15 Identity = 62/203 (30.54%), Postives = 96/203 (47.29%), Query Frame = 1
Query: 454 LFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY-KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTV-----LEEGISGLFKGLLPSSIKSGVVS--SLTFFFYEKF 1038
L GGVAG+ + P + ++ L Q Y + + ++I ++ EEG G FRG + IFP +A+QF +Y K L + + Q E L+N L SG++ G + YPLDL K RL +Q + K S+ I + + LE G+ GL++G+ P+S+ GVV +L F YE+
Sbjct: 27 LAGGVAGAVSRTVVSPFERVKILLQVQSSTTSYNRGIFSSIRQVYHEEGTKGLFRGNGLNCIRIFPYSAVQFVVYEACKKKLFHVNGNNGQ--------EQLTN--TQRLFSGALCGGCSVVATYPLDLIKTRLSIQTANLSSLNRSK-AKSISKPPGIWQLLSETYRLEGGLRGLYRGVWPTSL--GVVPYVALNFAVYEQL 216 HSP 3 Score: 67.0106 bits (162), Expect = 7.122e-13 Identity = 52/197 (26.40%), Postives = 92/197 (46.70%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQA-------SKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALY-----SKTGYSRTSTN--NLLFGGVAGSTATLFCQPIDVLRTR--LVSQGEYK---KYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSL 696
+GAL G + T PLD++K R +Q +S S K+I + S+ LE G+ G ++G P + + A+ F +E L S +N L G ++G A P D+LR R +++ G + +Y S+ +A++ + EGV G+++GL + + P TA+ + +Y + S+
Sbjct: 128 SGALCGGCSVVATYPLDLIKTRLSIQTANLS-SLNRSKAKSISKPPGIWQLLSETYRLEGGLRGLYRGVWPTSLGVVPYVALNFAVYEQLREFGVNSSDAQPSWKSNLYKLTIGAISGGVAQTITYPFDLLRRRFQVLAMGGNELGFRYTSVWDALVTIGRAEGVSGYYKGLAANLFKVVPSTAVSWLVYEVVCDSV 323 HSP 4 Score: 46.9802 bits (110), Expect = 2.256e-6 Identity = 33/101 (32.67%), Postives = 51/101 (50.50%), Query Frame = 1
Query: 748 EHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
+ L DS A ++G VAG V++TV+ P + K LQVQ + ++ + SIR+ EEG GLF+G + I+ S++ F YE K +
Sbjct: 16 DFLKQDSNIAFLAGGVAGAVSRTVVSPFERVKILLQVQSSTTS--------YNRGIFSSIRQVYHEEGTKGLFRGNGLNCIRIFPYSAVQFVVYEACKKKL 108
BLAST of slc25a-20 vs. Ensembl Nematostella
Match: EDO29391 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3S4]) HSP 1 Score: 231.876 bits (590), Expect = 1.127e-73 Identity = 133/324 (41.05%), Postives = 183/324 (56.48%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEP-------ISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGY---SRT------STNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEK----FSKMI 1050
++T G+++G RLI QPLDVLKIRFQLQVEP I+ Y + QA K ++ EEG WKGHVPAQ LS+V+G QF FEA ++K Y RT + + G +G A + QP+DV+RTRLV+QGE K Y SL A M EG FF+GL P++L IFP + +QFG Y LK+ + + I+V D + +L G+++G+++K VI P D+ K+R+QVQGFE+ARQ FG+ + R + EEG GLFKGL PS++K+ V + F YE+ F K+I
Sbjct: 13 LTTAQCGICGSVAGAFTRLIAQPLDVLKIRFQLQVEPTFQPTFQITTKVTTGKYTGVWQAGKLVFEEEGFAALWKGHVPAQALSVVYGYFQFTCFEA-FTKAAYFISPRTMEKKYKPATHFMCGAFSGCAAAVMAQPLDVIRTRLVAQGEPKIYNSLLQAARVMYKGEGPTVFFKGLTPSLLQIFPYSGLQFGSYSLLKTIW-------DHVFDIKV------TDVIESLTCGALSGMISKAVILPFDIIKKRIQVQGFEEARQSFGRVQQYDGVKDCFRTILKEEGAMGLFKGLAPSTLKAAVTVGIMFCTYEQCLHLFRKLI 322
BLAST of slc25a-20 vs. Ensembl Nematostella
Match: EDO34426 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPF1]) HSP 1 Score: 132.494 bits (332), Expect = 1.092e-35 Identity = 101/316 (31.96%), Postives = 153/316 (48.42%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSK------TGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFF-----RGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI**IYFFCWR 1077
AG ++G + R PL+ +KI Q+QV K+ +K + +I EEGI G++KG+ + AVQF +E Y K +T L+ G +AG T+ P+D++RTRL +QG +KY+ + +A + EEG GFF RGLVPT + I P + F +Y LK L S + S+Q + + L+ GS+AG V++T YPLD+ +RR+Q++G Y +H+ V EG GL+KG+ P+ +K + F YE SK +Y WR
Sbjct: 40 AGGIAGAVSRTSVSPLERVKILLQIQV-------KNPKFKGVLPTLIQIGKEEGILGYFKGNGTNVIRIFPYSAVQFAAYEE-YKKLLNIPDDPEHQTPIKRLVAGAMAGVTSITATYPLDLIRTRLSAQGADRKYRGIVHAFRTILNEEG--GFFSGCLYRGLVPTAMGIAPYVGLNFAVYETLKGFLFSTVMASSQGASLTNIRKDRELPVNFKLMCGSLAGAVSQTATYPLDVVRRRMQMKGIRADFAY-------KSTLHAFSSIVKLEGFRGLYKGMWPNILKVAPSVGIQFAAYE-LSKSF--LYSNKWR 335 HSP 2 Score: 95.5153 bits (236), Expect = 3.650e-22 Identity = 63/198 (31.82%), Postives = 105/198 (53.03%), Query Frame = 1
Query: 448 NLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEG--ISG-LFKGLLPSSIKSGVVSSLTFFFYE 1032
+LL GG+AG+ + P++ ++ L Q + K+K + ++++ EEG+ G+F+G V+ IFP +A+QF Y + K L+ +Q + R LV+G++AG+ + T YPLDL + RL QG + R+Y G +VH+ R + EEG SG L++GL+P+++ L F YE
Sbjct: 37 HLLAGGIAGAVSRTSVSPLERVKILLQIQVKNPKFKGVLPTLIQIGKEEGILGYFKGNGTNVIRIFPYSAVQFAAYEEYKKLLNIPDDPEHQTPIKR-------------LVAGAMAGVTSITATYPLDLIRTRLSAQGAD--RKYRG-------IVHAFRTILNEEGGFFSGCLYRGLVPTAMGIAPYVGLNFAVYE 212 HSP 3 Score: 67.0106 bits (162), Expect = 1.723e-12 Identity = 59/214 (27.57%), Postives = 89/214 (41.59%), Query Frame = 1
Query: 136 HMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIF---GFWKGHVPAQYLSIVFGAVQFRTFEAL---------YSKTGYSRTSTNN---------LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKK--YKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMR 708
H + AGA++G T PLD+++ R Q D Y+ I A + I EEG F ++G VP + + F +E L S G S T+ L+ G +AG+ + P+DV+R R+ +G YKS +A + EG G ++G+ P +L + P IQF Y KS L S +
Sbjct: 127 HQTPIKRLVAGAMAGVTSITATYPLDLIRTRLSAQGA-------DRKYRGIVHAFRTILNEEGGFFSGCLYRGLVPTAMGIAPYVGLNFAVYETLKGFLFSTVMASSQGASLTNIRKDRELPVNFKLMCGSLAGAVSQTATYPLDVVRRRMQMKGIRADFAYKSTLHAFSSIVKLEGFRGLYKGMWPNILKVAPSVGIQFAAYELSKSFLYSNK 333
BLAST of slc25a-20 vs. Ensembl Nematostella
Match: EDO34326 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPL4]) HSP 1 Score: 115.161 bits (287), Expect = 2.104e-29 Identity = 87/308 (28.25%), Postives = 144/308 (46.75%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKD-----FKDSYYKN-IKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNL---LFGGVA-GSTATLFCQPIDVLRTRL--VSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSK 1044
F G LS +T P++V+KIR QL E SK F++ YYK I+ R+Y EEG+ G ++G PA ++ + + +E + + G + +++ L + GV+ G + P D+++ R V GE YK++ +A K++ +EG G + G+ PTV + Q Y K L + AE + L S VAG VA V P+D+ + R Q +D + + +L ++ KTV EGI L+KG P+ ++G+ + + FF YE+ +
Sbjct: 12 FVLGGLSCMTATTVTNPIEVVKIRMQLDNELGSKHNSKDIFRERYYKGLIRTGLSRVYREEGVRGLYRGIFPALLRQAIYSSTRLGAYEPIKNLLGATDSTSAALWKKIVAGVSSGVIGSAIATPTDLVKIRFQAVKIGETIPYKNMFHAFYKIAKKEGFLGLWTGMKPTVKRAACISGTQIPTYDHTKHLL--------------LNAELMREGVALHLASALVAGFVATCVASPVDIVRTRFMTQP-KDTKGRPLVYQGTLDCIY---KTVRHEGILALYKGFFPNWTRTGLDTIIIFFVYERLRR 301
BLAST of slc25a-20 vs. Ensembl Nematostella
Match: EDO31058 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZ11]) HSP 1 Score: 111.694 bits (278), Expect = 2.452e-28 Identity = 87/295 (29.49%), Postives = 143/295 (48.47%), Query Frame = 1
Query: 169 ALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIK--QASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ-GEYKKYKSLTNAILKMSAEE-GVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQ-SNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQG-FEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYE 1032
LS R PL+ LKI Q + +YK +K A + IY EG+ ++KG+ + +GAVQF ++E S + N L+ G +AG TA P+D++R+RL Q + + Y ++T I +S +E G ++G VPT+L I P I F ++ +K+ RI +N N LS + V G VAG V++T+ YPLD+ +RR+Q+ G D +Y ++++ ++GI GL++GL + ++ ++ F YE
Sbjct: 7 GLSTCCARTTMAPLERLKILLQAN---------NRHYKGMKVLTAFRAIYRNEGLLAYFKGNGAMMLRTFPYGAVQFLSYEHYSKVLQTSSPAINKLVAGSLAGMTACACTYPLDMVRSRLAFQVAQDQGYTTITQTIRCISVKEGGPKALYKGFVPTLLTIVPAMGIGFYMFETMKAYFLETRIAFTNTN--PDTLCPELS--IIGGFVCGGVAGAVSQTIAYPLDVVRRRMQLAGAVPDGHKYN-------TCINTLVNVYKDDGIRRGLYRGLSINYLRVCPQVAIMFGVYE 281 HSP 2 Score: 81.2629 bits (199), Expect = 2.088e-17 Identity = 66/214 (30.84%), Postives = 102/214 (47.66%), Query Frame = 1
Query: 127 KVLHMSTNS--AFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEG-IFGFWKGHVPAQYLSIV----FGAVQFRTFEALYSKTGYSRTSTN------------NLLFGGVAGSTATLFCQPIDVLRTRLVSQG---EYKKYKSLTNAILKMSAEEGVY-GFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLS 699
KVL S+ + AG+L+G T PLD+++ R QV +D Y I Q + I ++EG +KG VP L+IV G F T +A + +T + T+TN + GGVAG+ + P+DV+R R+ G + KY + N ++ + ++G+ G +RGL L + PQ AI FG+Y K L+
Sbjct: 82 KVLQTSSPAINKLVAGSLAGMTACACTYPLDMVRSRLAFQVA------QDQGYTTITQTIRCISVKEGGPKALYKGFVPT-LLTIVPAMGIGFYMFETMKAYFLETRIAFTNTNPDTLCPELSIIGGFVCGGVAGAVSQTIAYPLDVVRRRMQLAGAVPDGHKYNTCINTLVNVYKDDGIRRGLYRGLSINYLRVCPQVAIMFGVYEVTKQFLN 288 HSP 3 Score: 64.3142 bits (155), Expect = 1.135e-11 Identity = 48/192 (25.00%), Postives = 84/192 (43.75%), Query Frame = 1
Query: 457 FGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
F G++ A P++ L+ L + + K + A + EG+ +F+G +L FP A+QF Y L + S+ ++N LV+GS+AG+ A YPLD+ + RL Q +D Q + ++R + +V E G L+KG +P+ + + F+ +E
Sbjct: 5 FLGLSTCCARTTMAPLERLKILLQANNRHYKGMKVLTAFRAIYRNEGLLAYFKGNGAMMLRTFPYGAVQFLSYEHYSKVLQT------------------SSPAINKLVAGSLAGMTACACTYPLDMVRSRLAFQVAQD--QGYTTITQTIRCI-----SVKEGGPKALYKGFVPTLLTIVPAMGIGFYMFE 171
BLAST of slc25a-20 vs. Ensembl Nematostella
Match: EDO37153 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGK5]) HSP 1 Score: 109.768 bits (273), Expect = 1.507e-27 Identity = 85/314 (27.07%), Postives = 153/314 (48.73%), Query Frame = 1
Query: 145 TNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIK--QASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFE--ALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQ-GEYKKYKSLTNAILKM-SAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSV----NALVSGSVAGIVAKTVIYPLDLAKRRLQVQG-FEDARQYFGKFHHSLRMVHSIRKTVLEEGI-SGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
T + F AG LS + T PL+ LKI FQ Q + +YKN+ A K IY +EG+ G++KG+ +G++QF ++E L + + + ++ GG+AG TA P+D++R+RL Q + Y + + ++ E G+ +RG PT L + P I F + K +M+ + R++ E + ++V L+ G++AG ++T+ YPLD+ +RR+Q+ G D +Y +++ E+GI GL++GL + ++ ++ F YE +++
Sbjct: 6 TINTFVAGGLSTCCAKTTTAPLERLKILFQAQ---------NKHYKNMSVFGALKAIYKKEGLQGYYKGNGAMMVRVFPYGSIQFVSYEQYKLLFENALQNSHLSKIVAGGLAGLTACSCTYPLDIVRSRLAFQVADEHTYCGICQTVKQIFMTEGGMVALYRGFTPTSLSMIPAVGIGFYAFESFKDFFVAMK-----GVLTRIHPE--TGETVLTAPGGLLCGALAGATSQTLAYPLDVVRRRMQLAGTVADGHKYS-------TCINTFISVYTEDGIRRGLYRGLSINYLRVCPQVAVMFAVYEVVKQLL 296 HSP 2 Score: 80.1073 bits (196), Expect = 5.837e-17 Identity = 59/209 (28.23%), Postives = 95/209 (45.45%), Query Frame = 1
Query: 133 LHMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEG-IFGFWKGHVPAQYLSIVFGAVQFRTFEA--------------LYSKTGYS-RTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQG---EYKKYKSLTNAILKMSAEEGVY-GFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLS 699
L S S AG L+G T PLD+++ R QV + Y I Q K+I++ EG + ++G P I + F FE+ ++ +TG + T+ LL G +AG+T+ P+DV+R R+ G + KY + N + + E+G+ G +RGL L + PQ A+ F +Y +K L+
Sbjct: 95 LQNSHLSKIVAGGLAGLTACSCTYPLDIVRSRLAFQVA------DEHTYCGICQTVKQIFMTEGGMVALYRGFTPTSLSMIPAVGIGFYAFESFKDFFVAMKGVLTRIHPETGETVLTAPGGLLCGALAGATSQTLAYPLDVVRRRMQLAGTVADGHKYSTCINTFISVYTEDGIRRGLYRGLSINYLRVCPQVAVMFAVYEVVKQLLT 297 HSP 3 Score: 72.4034 bits (176), Expect = 2.153e-14 Identity = 51/219 (23.29%), Postives = 101/219 (46.12%), Query Frame = 1
Query: 439 STNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEE-GISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI**IYFFCWRNLLLR 1092
+ N + GG++ A P++ L+ +Q ++ K S+ A+ + +EG+ G+++G ++ +FP +IQF Y Q K ++ L N ++ +V+G +AG+ A + YPLD+ + RL Q D Y G + ++++ + E G+ L++G P+S+ + F+ +E F +F + +L R
Sbjct: 6 TINTFVAGGLSTCCAKTTTAPLERLKILFQAQNKHYKNMSVFGALKAIYKKEGLQGYYKGNGAMMVRVFPYGSIQFVSYEQYK----------------LLFENALQNSHLSKIVAGGLAGLTACSCTYPLDIVRSRLAFQ-VADEHTYCG-------ICQTVKQIFMTEGGMVALYRGFTPTSLSMIPAVGIGFYAFESFKD-----FFVAMKGVLTR 195
BLAST of slc25a-20 vs. Ensembl Medaka
Match: slc25a19 (solute carrier family 25 member 19 [Source:NCBI gene;Acc:101168214]) HSP 1 Score: 240.736 bits (613), Expect = 9.165e-77 Identity = 132/313 (42.17%), Postives = 183/313 (58.47%), Query Frame = 1
Query: 127 KVLHMSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL----YSKTGY-SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
K +S A AG+ +G + R + P DV+KIRFQLQ+E +S + Y + QA + IY EEG+ FWKGHVPAQ LSI +GAVQF +FE L Y T Y S+++ + GG+A +AT+ CQP+D LRTR SQGE K Y++L +A+ M EG FFRGL PT++ ++P +QF Y+ K L + S S+ +LV GS AG+++KT+ YP DL K+RLQV GFE AR FG+ ++ + + EEG G FKGL PS +K+ + + TFF+YE F +I
Sbjct: 9 KEAAISPKEAVLAGSAAGMVTRALISPFDVVKIRFQLQIERVSALRPEGKYAGLFQAFRCIYSEEGLSAFWKGHVPAQLLSICYGAVQFTSFEVLTKMVYKLTPYDSQSAGVHFFCGGLAACSATVVCQPLDTLRTRFASQGEPKVYRNLRHAVSTMWRTEGPLTFFRGLSPTLVAVYPYAGLQFFFYNVSKKWLGPPSKGGD------------SGGSLKSLVCGSGAGVISKTITYPFDLFKKRLQVGGFEAARARFGQVRRYSGLMDCMFQIAKEEGFRGFFKGLSPSLVKAALSTGFTFFWYEFFINLI 309
BLAST of slc25a-20 vs. Ensembl Medaka
Match: SLC25A42 (mitochondrial coenzyme A transporter SLC25A42 [Source:NCBI gene;Acc:101165762]) HSP 1 Score: 117.087 bits (292), Expect = 1.249e-29 Identity = 88/303 (29.04%), Postives = 140/303 (46.20%), Query Frame = 1
Query: 148 NSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQF-------RTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEG-ISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
NS F+ GAL+G + + PLD KI FQ+ S K++Y + R YL++G W+G+ I + A+QF R Y G LL G +AG+TA + P+DV+R R+ + + Y ++ + ++S EEG+ FRG PT+L + P + F Y LK L + R Y S L G+ AG++ ++ YPLD+ +RR+Q G H + ++R+ V EEG I GL+KGL + +K + ++F ++
Sbjct: 38 NSLFS-GALAGAVAKTAVAPLDRTKIIFQVSSNRFSA--KEAY-----RLIYRTYLKDGFLSLWRGNSATMVRVIPYAAIQFCAHEQYKRLLGGYYGFQGKVLPPVPRLLAGSLAGTTAAMLTYPLDVVRARMAVTPK-EMYSNILHVFARISQEEGIKTLFRGFTPTILGVVPYAGLSFFTYETLK-KLHAERTGRAHPY------------SYERLTFGACAGLIGQSASYPLDVVRRRMQTAGVTG--------HTYGTIFGTMREIVSEEGFIRGLYKGLSMNWVKGPIAVGISFTTFD 310 HSP 2 Score: 69.707 bits (169), Expect = 7.300e-13 Identity = 57/208 (27.40%), Postives = 93/208 (44.71%), Query Frame = 1
Query: 430 SRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY--KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
+R+ N+L G +AG+ A P+D RT+++ Q ++ K I + ++G +RG T++ + P AIQF + Q K L Q V+ V L++GS+AG A + YPLD+ + R+ V E ++H + EEGI LF+G P+ + + L+FF YE K+
Sbjct: 33 TRSVLNSLFSGALAGAVAKTAVAPLD--RTKIIFQVSSNRFSAKEAYRLIYRTYLKDGFLSLWRGNSATMVRVIPYAAIQFCAHEQYKRLLGGY--YGFQGKVL---------PPVPRLLAGSLAGTTAAMLTYPLDVVRARMAVTPKEMYSN----------ILHVFARISQEEGIKTLFRGFTPTILGVVPYAGLSFFTYETLKKL 217
BLAST of slc25a-20 vs. Ensembl Medaka
Match: ENSORLT00000031788.1 (mitochondrial coenzyme A transporter SLC25A42-like [Source:NCBI gene;Acc:101167609]) HSP 1 Score: 117.087 bits (292), Expect = 1.528e-29 Identity = 89/309 (28.80%), Postives = 149/309 (48.22%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIY---LEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNN-------LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEG-ISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
+S + GA +G + + + PLD KI FQ+ SK F + ++A + IY L++G+ W+G+ + + A+QF + E G S LL G +AG+TA P+DV+R R+ + + Y ++ + +++S EEGV +RG PT+L + P I F Y LK L + R + Q Y H LV G+ AG++ ++ YPLD+ +RR+Q G G + ++ V +IR+ V +EG + GL+KGL + +K V ++F ++
Sbjct: 35 LSAVDSLLCGAFAGAVAKTVIAPLDRTKIIFQVS----SKRF------SAREAFRLIYCTYLKDGLLSLWRGNSATMVRVMPYAAIQFCSHEQYKKLLGGDYGSQERALPPFPRLLAGSLAGTTAATLTYPLDVVRARMAVTAK-EMYSNIMHVFVRISQEEGVRTLYRGFTPTILGVIPYAGITFFTYETLK-KLHAERTKRCQPY------PH------ERLVFGACAGLIGQSASYPLDVVRRRMQTAGVT------GSSYSTI--VGTIREIVTKEGVVRGLYKGLSMNWLKGPVAVGISFTAFD 311 HSP 2 Score: 68.9366 bits (167), Expect = 1.007e-12 Identity = 55/206 (26.70%), Postives = 95/206 (46.12%), Query Frame = 1
Query: 436 TSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY--KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
++ ++LL G AG+ A P+D RT+++ Q K++ + I ++G+ +RG T++ + P AIQF + Q K L +Q + + L++GS+AG A T+ YPLD+ + R+ V E Y H +R+ EEG+ L++G P+ + + +TFF YE K+
Sbjct: 36 SAVDSLLCGAFAGAVAKTVIAPLD--RTKIIFQVSSKRFSAREAFRLIYCTYLKDGLLSLWRGNSATMVRVMPYAAIQFCSHEQYKKLLGG--DYGSQERALPPFPR---------LLAGSLAGTTAATLTYPLDVVRARMAVTAKE---MYSNIMHVFVRISQ-------EEGVRTLYRGFTPTILGVIPYAGITFFTYETLKKL 218
BLAST of slc25a-20 vs. Ensembl Medaka
Match: ENSORLT00000000948.2 (mitochondrial coenzyme A transporter SLC25A42-like [Source:NCBI gene;Acc:101167609]) HSP 1 Score: 117.087 bits (292), Expect = 1.528e-29 Identity = 89/309 (28.80%), Postives = 149/309 (48.22%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIY---LEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNN-------LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEG-ISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
+S + GA +G + + + PLD KI FQ+ SK F + ++A + IY L++G+ W+G+ + + A+QF + E G S LL G +AG+TA P+DV+R R+ + + Y ++ + +++S EEGV +RG PT+L + P I F Y LK L + R + Q Y H LV G+ AG++ ++ YPLD+ +RR+Q G G + ++ V +IR+ V +EG + GL+KGL + +K V ++F ++
Sbjct: 35 LSAVDSLLCGAFAGAVAKTVIAPLDRTKIIFQVS----SKRF------SAREAFRLIYCTYLKDGLLSLWRGNSATMVRVMPYAAIQFCSHEQYKKLLGGDYGSQERALPPFPRLLAGSLAGTTAATLTYPLDVVRARMAVTAK-EMYSNIMHVFVRISQEEGVRTLYRGFTPTILGVIPYAGITFFTYETLK-KLHAERTKRCQPY------PH------ERLVFGACAGLIGQSASYPLDVVRRRMQTAGVT------GSSYSTI--VGTIREIVTKEGVVRGLYKGLSMNWLKGPVAVGISFTAFD 311 HSP 2 Score: 68.9366 bits (167), Expect = 1.007e-12 Identity = 55/206 (26.70%), Postives = 95/206 (46.12%), Query Frame = 1
Query: 436 TSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY--KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
++ ++LL G AG+ A P+D RT+++ Q K++ + I ++G+ +RG T++ + P AIQF + Q K L +Q + + L++GS+AG A T+ YPLD+ + R+ V E Y H +R+ EEG+ L++G P+ + + +TFF YE K+
Sbjct: 36 SAVDSLLCGAFAGAVAKTVIAPLD--RTKIIFQVSSKRFSAREAFRLIYCTYLKDGLLSLWRGNSATMVRVMPYAAIQFCSHEQYKKLLGG--DYGSQERALPPFPR---------LLAGSLAGTTAATLTYPLDVVRARMAVTAKE---MYSNIMHVFVRISQ-------EEGVRTLYRGFTPTILGVIPYAGITFFTYETLKKL 218
BLAST of slc25a-20 vs. Ensembl Medaka
Match: ENSORLT00000035929.1 (mitochondrial coenzyme A transporter SLC25A42-like [Source:NCBI gene;Acc:101167609]) HSP 1 Score: 117.087 bits (292), Expect = 1.528e-29 Identity = 89/309 (28.80%), Postives = 149/309 (48.22%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIY---LEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNN-------LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEG-ISGLFKGLLPSSIKSGVVSSLTFFFYE 1032
+S + GA +G + + + PLD KI FQ+ SK F + ++A + IY L++G+ W+G+ + + A+QF + E G S LL G +AG+TA P+DV+R R+ + + Y ++ + +++S EEGV +RG PT+L + P I F Y LK L + R + Q Y H LV G+ AG++ ++ YPLD+ +RR+Q G G + ++ V +IR+ V +EG + GL+KGL + +K V ++F ++
Sbjct: 35 LSAVDSLLCGAFAGAVAKTVIAPLDRTKIIFQVS----SKRF------SAREAFRLIYCTYLKDGLLSLWRGNSATMVRVMPYAAIQFCSHEQYKKLLGGDYGSQERALPPFPRLLAGSLAGTTAATLTYPLDVVRARMAVTAK-EMYSNIMHVFVRISQEEGVRTLYRGFTPTILGVIPYAGITFFTYETLK-KLHAERTKRCQPY------PH------ERLVFGACAGLIGQSASYPLDVVRRRMQTAGVT------GSSYSTI--VGTIREIVTKEGVVRGLYKGLSMNWLKGPVAVGISFTAFD 311 HSP 2 Score: 68.9366 bits (167), Expect = 1.007e-12 Identity = 55/206 (26.70%), Postives = 95/206 (46.12%), Query Frame = 1
Query: 436 TSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY--KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
++ ++LL G AG+ A P+D RT+++ Q K++ + I ++G+ +RG T++ + P AIQF + Q K L +Q + + L++GS+AG A T+ YPLD+ + R+ V E Y H +R+ EEG+ L++G P+ + + +TFF YE K+
Sbjct: 36 SAVDSLLCGAFAGAVAKTVIAPLD--RTKIIFQVSSKRFSAREAFRLIYCTYLKDGLLSLWRGNSATMVRVMPYAAIQFCSHEQYKKLLGG--DYGSQERALPPFPR---------LLAGSLAGTTAATLTYPLDVVRARMAVTAKE---MYSNIMHVFVRISQ-------EEGVRTLYRGFTPTILGVIPYAGITFFTYETLKKL 218
BLAST of slc25a-20 vs. Planmine SMEST
Match: SMESG000047064.1 (SMESG000047064.1) HSP 1 Score: 239.195 bits (609), Expect = 2.749e-76 Identity = 138/308 (44.81%), Postives = 187/308 (60.71%), Query Frame = 1
Query: 163 AGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFE-------ALYSK---TGYS-RTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSS-MRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
AGA GF+ R + QPLDV+KIRFQLQVEPI + D YK I QA K IY EEG+ FWKGH+ +Q ++ F VQF T+E L S+ TG S S+ L G +AGSTAT+F QP DV RTR +QGE K Y +L+NAI+ + EG+ G FRGL+P++L++ PQT QF Y+ + L+ + + Q+ + ++ + + + +SG +AG +AK +YP+D+ K+R+QVQGFE AR FGK H S +V I+ L EGI G FKGL S IKS + L F YE+ I
Sbjct: 20 AGASGGFVSRAVVQPLDVVKIRFQLQVEPI-QTITDGKYKGIAQAIKCIYKEEGLKAFWKGHLSSQIQAVSFSLVQFSTYELCKVYFWPLISESPSTGKSVLKSSKTFLLGSLAGSTATIFTQPFDVFRTRFAAQGEPKTYPNLSNAIIMIIKTEGLRGLFRGLIPSLLLVAPQTGFQFFFYNLSNNVLTKFFKTKGKQDNLFEIHKIGI----LQSALSGGIAGSMAKICVYPVDIIKKRMQVQGFEKARSKFGKLHVSKGVVKCIKVIFLVEGIGGYFKGLKTSVIKSFISLGLRFSVYEQVCNGI 322 HSP 2 Score: 73.1738 bits (178), Expect = 3.134e-14 Identity = 56/214 (26.17%), Postives = 98/214 (45.79%), Query Frame = 1
Query: 139 MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFE---ALYSKTGYSRTSTNNL------------LFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKY---------KSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMR 708
+ ++ F G+L+G + TQP DV + RF Q EP + Y N+ A I EG+ G ++G +P+ L QF + + +K ++ +NL L GG+AGS A + P+D+++ R+ QG K K + I + EG+ G+F+GL +V+ F ++F +Y Q+ + + ++R
Sbjct: 120 LKSSKTFLLGSLAGSTATIFTQPFDVFRTRFAAQGEPKT-------YPNLSNAIIMIIKTEGLRGLFRGLIPSLLLVAPQTGFQFFFYNLSNNVLTKFFKTKGKQDNLFEIHKIGILQSALSGGIAGSMAKICVYPVDIIKKRMQVQGFEKARSKFGKLHVSKGVVKCIKVIFLVEGIGGYFKGLKTSVIKSFISLGLRFSVYEQVCNGILNLR 326
BLAST of slc25a-20 vs. Planmine SMEST
Match: SMESG000015827.1 (SMESG000015827.1) HSP 1 Score: 207.223 bits (526), Expect = 5.374e-64 Identity = 125/310 (40.32%), Postives = 173/310 (55.81%), Query Frame = 1
Query: 142 STNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSKTGYS--RTST------NNLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYH----QLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEK 1035
+ SAF AGA SGF+ R + QPLDVLKIR QLQVEP+ K F Y I IY EEG WKGH+ Q +I F +QF T+ L K +S R ST +N G ++G A QP+D+LRTR V+QGE K Y+SL +A + +EG +RG +P++L+I PQ+ +QF Y+ + S S R N+N L V ++ SG +AG ++K +++PLD+ K+R+QVQGFE+AR+ FGK H S + EEG + KGL P+ +KS + L F YE+
Sbjct: 12 ARESAF-AGAASGFVSRAMVQPLDVLKIRLQLQVEPLKKKFGK--YHGIFPTICCIYKEEGFKALWKGHMCGQIHAISFSMIQFYTYTCLIEKYKFSLQRHSTFSEKLISNFFCGSLSGILAISLTQPLDILRTRFVAQGEPKLYRSLYHAAASIVQKEGFLTLYRGSIPSILLIAPQSGLQFLFYNFSNAVIGSIFSLFRFYENKN--------ELKIGVVQSIASGGMAGALSKVLVFPLDIVKKRMQVQGFEEARKKFGKLHRSQGFIRCFGVIYREEGWTAFTKGLKPTLLKSFISIGLKFSVYEQ 310 HSP 2 Score: 70.8626 bits (172), Expect = 1.990e-13 Identity = 57/211 (27.01%), Postives = 101/211 (47.87%), Query Frame = 1
Query: 151 SAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPA----------QYL-----SIVFGAVQFRTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQG---EYKKYKSL--TNAILK----MSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRI 711
S F G+LSG + +TQPLD+L+ RF Q EP Y+++ A+ I +EG ++G +P+ Q+L + V G++ F F +K ++ GG+AG+ + + P+D+++ R+ QG KK+ L + ++ + EEG F +GL PT+L F ++F +Y Q L+ +++
Sbjct: 118 SNFFCGSLSGILAISLTQPLDILRTRFVAQGEP-------KLYRSLYHAAASIVQKEGFLTLYRGSIPSILLIAPQSGLQFLFYNFSNAVIGSI-FSLFRFYENKNELKIGVVQSIASGGMAGALSKVLVFPLDIVKKRMQVQGFEEARKKFGKLHRSQGFIRCFGVIYREEGWTAFTKGLKPTLLKSFISIGLKFSVYEQAYQLLTGVQM 320 HSP 3 Score: 49.6766 bits (117), Expect = 1.734e-6 Identity = 29/90 (32.22%), Postives = 49/90 (54.44%), Query Frame = 1
Query: 760 NDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFY 1029
N + + +G+ +G V++ ++ PLD+ K RLQ+Q E ++ FGK+H + I K EEG L+KG + I + S + F+ Y
Sbjct: 10 NSARESAFAGAASGFVSRAMVQPLDVLKIRLQLQ-VEPLKKKFGKYHGIFPTICCIYK---EEGFKALWKGHMCGQIHAISFSMIQFYTY 95
BLAST of slc25a-20 vs. Planmine SMEST
Match: SMESG000074820.1 (SMESG000074820.1) HSP 1 Score: 98.2117 bits (243), Expect = 2.957e-22 Identity = 76/301 (25.25%), Postives = 135/301 (44.85%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEALYSK--TGYSRTSTNNLLF------GGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEK 1035
AG ++G + R T PLD LK+ Q + I A K + E G+ W+G+ A++F +E+ Y K +++ T N+ G +AG+TA P++VL+TR+ + + +Y S+ + K+ EG+ FFRG VP +L I P I +Y K +L I +++ +Y +L SG+V+ + + YPL L + +LQ D + + M + R + EG+ G ++G+ P+ +K S+++ +E+
Sbjct: 188 LVAGGIAGCVSRTATAPLDRLKVTMQAHGAKAAN-------HGIINAIKYMVNEGGVSSLWRGNGVNCIKIAPESAIKFLAYES-YKKLIVNFTQNETGNVTLGEKFVAGAMAGATAQTLIYPMEVLKTRMCLR-KSGQYSSIFDCAKKVYINEGIQVFFRGYVPNILGIIPYAGIDLALYETFK-NLYLKSINHDKSASTPIYV---------SLCSGAVSSVCGQVATYPLALVRTKLQAMSKSDGK---------ISMKNVSRDIIKNEGVLGFYRGIGPNMLKVIPAVSISYAVFEQ 460 HSP 2 Score: 83.1889 bits (204), Expect = 3.404e-17 Identity = 50/185 (27.03%), Postives = 95/185 (51.35%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQ---FRTFEALYSKT-GYSRTSTN----NLLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLK 687
F AGA++G + + P++VLK R L+ K Y +I +K++Y+ EGI F++G+VP I + + + TF+ LY K+ + ++++ +L G V+ + P+ ++RT+L + + S+ N + EGV GF+RG+ P +L + P +I + ++ Q +
Sbjct: 286 FVAGAMAGATAQTLIYPMEVLKTRMCLR--------KSGQYSSIFDCAKKVYINEGIQVFFRGYVPNILGIIPYAGIDLALYETFKNLYLKSINHDKSASTPIYVSLCSGAVSSVCGQVATYPLALVRTKLQAMSKSDGKISMKNVSRDIIKNEGVLGFYRGIGPNMLKVIPAVSISYAVFEQTR 462 HSP 3 Score: 68.1662 bits (165), Expect = 2.809e-12 Identity = 50/199 (25.13%), Postives = 88/199 (44.22%), Query Frame = 1
Query: 451 LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKM 1047
L+ GG+AG + P+D L+ + + G + NAI M E GV +RG + I P++AI+F Y K + + N + V+G++AG A+T+IYP+++ K R+ ++ + QY F + +K + EGI F+G +P+ + + + YE F +
Sbjct: 188 LVAGGIAGCVSRTATAPLDRLKVTMQAHGAKAANHGIINAIKYMVNEGGVSSLWRGNGVNCIKIAPESAIKFLAYESYKKLIVNFTQNETGNVTLG-----------EKFVAGAMAGATAQTLIYPMEVLKTRMCLR---KSGQYSSIFDCA-------KKVYINEGIQVFFRGYVPNILGIIPYAGIDLALYETFKNL 365 HSP 4 Score: 50.0618 bits (118), Expect = 1.885e-6 Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 1
Query: 748 EHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
E L + LV+G +AG V++T PLD K +Q G + A +H + +++I+ V E G+S L++G + IK S++ F YE + K+I
Sbjct: 178 EKLKGIAWKTLVAGGIAGCVSRTATAPLDRLKVTMQAHGAKAA-------NHGI--INAIKYMVNEGGVSSLWRGNGVNCIKIAPESAIKFLAYESYKKLI 269
BLAST of slc25a-20 vs. Planmine SMEST
Match: SMESG000009142.1 (SMESG000009142.1) HSP 1 Score: 89.3521 bits (220), Expect = 6.221e-20 Identity = 84/308 (27.27%), Postives = 132/308 (42.86%), Query Frame = 1
Query: 154 AFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGH------VPAQYLSIVFGAVQFRTFEALYSKTGYSRTSTNNLLFGGVAGSTATLFCQPIDVLRTRLVSQGE---YKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSKMI 1050
+F AG + G ++ QPLD +K+R LQ PI K + YK++ + EG FGF+KG V + FG R + T ++ G ++G T P + ++ L QG KYK + + ++ E G+ ++G T+L P T F Y LK SL+ HLS + L++G AG+ V P D K RLQ A + GK+ + LR V R+ + EG+ GL++G+ P I++ ++ F YE K++
Sbjct: 11 SFIAGGVGGICTVVVGQPLDTIKVR--LQAMPIPKPGELPLYKSMADCFLQTLKNEGPFGFYKGMAAPLIGVTPMFAVCFFGYNLGRKLQVKDENTPLTKPQI--FAAGMLSGVFTTAIMTPGERVKCLLQVQGASSGPPKYKGPADVVKQLYKEGGIRSIYKGTAATLLRDIPATGTYFMSYEWLKESLTPEGGDK----------AHLS--AGRTLIAGGFAGMCNWLVCIPPDTLKSRLQT-----APE--GKYPNGLRSV--FREMMANEGLIGLYRGVSPVLIRAFPANAACFLGYEMALKLL 293
BLAST of slc25a-20 vs. Planmine SMEST
Match: SMESG000054493.1 (SMESG000054493.1) HSP 1 Score: 88.5817 bits (218), Expect = 1.559e-19 Identity = 81/312 (25.96%), Postives = 134/312 (42.95%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL--YSKTGYSRTSTNNLLF---GGVAGSTATLFCQPIDVLRTRLVSQGEYK------------KYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSLSSMRIQSNQNYVIRVYAEHLSNDSVNALVSGSVAGIVAKTVIYPLDLAKRRLQVQGFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFS 1041
+G +G I L+ PLD++KIRFQ+ E + YY + A +RI G G ++G +P S F + ++ Y + G + + L + ++G+ F PI V++TRL Q + + Y + N ++ EG+ G ++G +P L A+QF Y +K+ S + + N + + L+ G V+ +VA + YP + + RLQ + R Y G ++ I + EGI G +KGLLPS I+ S + F YE S
Sbjct: 15 LVSGMSAGIISTLVLHPLDLIKIRFQVN-EGTGVAIRPKYYGMV-DACRRIVKVNGFSGLYQGVMPNTVGSGCAWGFYFIIYNSMKEYQQKGNLKQELSKLEYMTMAALSGALTLAFTNPIWVVKTRLCLQYDKELFNTSKNNPKTVHYSGMINCFNQIYKTEGMRGLYKGFIPG-LFGTSHGAVQFVTYESMKNFYSQWYYRKDINTKLNTW---------EYLLLGCVSKLVAASTTYPYQVVRSRLQ----DQHRVYGG-------IIDVIFQLWKYEGIYGFYKGLLPSLIRVTPASGIVFVVYETMS 303 HSP 2 Score: 87.0409 bits (214), Expect = 5.235e-19 Identity = 62/194 (31.96%), Postives = 101/194 (52.06%), Query Frame = 1
Query: 157 FTAGALSGFIVRLITQPLDVLKIRFQLQVEP----ISKDF-KDSYYKNIKQASKRIYLEEGIFGFWKGHVPAQYLSIVFGAVQFRTFEAL---YSKTGYSR---TSTNN---LLFGGVAGSTATLFCQPIDVLRTRLVSQGEYKKYKSLTNAILKMSAEEGVYGFFRGLVPTVLMIFPQTAIQFGIYHQLKSSL 696
T ALSG + T P+ V+K R LQ + SK+ K +Y + +IY EG+ G +KG +P + GAVQF T+E++ YS+ Y + T N LL G V+ A P V+R+RL Q +++ Y + + I ++ EG+YGF++GL+P+++ + P + I F +Y + + L
Sbjct: 116 MTMAALSGALTLAFTNPIWVVKTRLCLQYDKELFNTSKNNPKTVHYSGMINCFNQIYKTEGMRGLYKGFIPGLF-GTSHGAVQFVTYESMKNFYSQWYYRKDINTKLNTWEYLLLGCVSKLVAASTTYPYQVVRSRL--QDQHRVYGGIIDVIFQLWKYEGIYGFYKGLLPSLIRVTPASGIVFVVYETMSTYL 306 HSP 3 Score: 59.6918 bits (143), Expect = 9.180e-10 Identity = 36/105 (34.29%), Postives = 56/105 (53.33%), Query Frame = 1
Query: 745 AEHLSNDSVNA--LVSGSVAGIVAKTVIYPLDLAKRRLQVQ---GFEDARQYFGKFHHSLRMVHSIRKTVLEEGISGLFKGLLPSSIKSGVVSSLTFFFYEKFSK 1044
+ ++SN +V LVSG AGI++ V++PLDL K R QV G +Y+G MV + R+ V G SGL++G++P+++ SG F Y +
Sbjct: 2 SANISNRTVQWEHLVSGMSAGIISTLVLHPLDLIKIRFQVNEGTGVAIRPKYYG-------MVDACRRIVKVNGFSGLYQGVMPNTVGSGCAWGFYFIIYNSMKE 99 The following BLAST results are available for this feature:
BLAST of slc25a-20 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of slc25a-20 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of slc25a-20 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of slc25a-20 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of slc25a-20 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010073 ID=SMED30010073|Name=slc25a-20|organism=Schmidtea mediterranea sexual|type=transcript|length=1126bpback to top protein sequence of SMED30010073-orf-1 >SMED30010073-orf-1 ID=SMED30010073-orf-1|Name=SMED30010073-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=305bp MSTNSAFTAGALSGFIVRLITQPLDVLKIRFQLQVEPISKDFKDSYYKNIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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