UPF0454 protein C12orf49-like

Overview
NameUPF0454 protein C12orf49-like
Smed IDSMED30010070
Length (bp)673
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of UPF0454 protein C12orf49-like (SMED30010070) t-SNE clustered cells

Violin plots show distribution of expression levels for UPF0454 protein C12orf49-like (SMED30010070) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of UPF0454 protein C12orf49-like (SMED30010070) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for UPF0454 protein C12orf49-like (SMED30010070) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010070

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30010070SMESG000061283.1 SmedASXL_000875SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30010070SMESG000061283.1 SmedASXL_000875SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Human
Match: C12orf49 (chromosome 12 open reading frame 49 [Source:HGNC Symbol;Acc:HGNC:26128])

HSP 1 Score: 106.301 bits (264), Expect = 6.899e-28
Identity = 56/131 (42.75%), Postives = 78/131 (59.54%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILA---CKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPIL 581
            N+C N   G  L+ DE+G +C R  +  NGCC+   +P      C  C  N CC+ YEYCVSCC+    + L +  L ++++A + ++  V   FE C+AKCRTSS SV HEN+YR+ I KYC G + P L
Sbjct:   73 NQCRNSIQGKHLITDELGYVCERKDLLVNGCCNVN-VPSTKQYCCDGCWPNGCCSAYEYCVSCCLQPNKQLLLERFLNRAAVAFQNLFMAVEDHFELCLAKCRTSSQSVQHENTYRDPIAKYCYGESPPEL 202
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Human
Match: C12orf49 (chromosome 12 open reading frame 49 [Source:HGNC Symbol;Acc:HGNC:26128])

HSP 1 Score: 50.0618 bits (118), Expect = 2.723e-8
Identity = 25/41 (60.98%), Postives = 29/41 (70.73%), Query Frame = 3 
Query:  459 VRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPIL 581
            V   FE C+AKCRTSS SV HEN+YR+ I KYC G + P L
Sbjct:    3 VEDHFELCLAKCRTSSQSVQHENTYRDPIAKYCYGESPPEL 43
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Fly
Match: CG15643 (gene:FBgn0030654 transcript:FBtr0074072)

HSP 1 Score: 98.9821 bits (245), Expect = 2.303e-25
Identity = 52/142 (36.62%), Postives = 80/142 (56.34%), Query Frame = 3 
Query:  150 LILNTSWMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRK--PIPILACKTCQ-SNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGI 566
            L+     ++      + RC N   G   + DE G +C R  V  NGCC+ +   I   +C+TC  S  CC +YEYCVSCC++   + L + +L+  +   K+++++V   FE C+ KCRT+S SV HEN YR+   K+C G+
Sbjct:   59 LLAPEEQLNRTRGDPEARCRNSVQGRQWLADERGFVCRREEVLTNGCCNLELPGIGYYSCRTCNTSTHCCGVYEYCVSCCLHPGQQPLLERVLQAPN-TPKYIFASVTDHFELCLVKCRTNSHSVEHENKYRDPAAKHCYGL 199
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Fly
Match: CG15643 (gene:FBgn0030654 transcript:FBtr0340349)

HSP 1 Score: 98.9821 bits (245), Expect = 2.303e-25
Identity = 52/142 (36.62%), Postives = 80/142 (56.34%), Query Frame = 3 
Query:  150 LILNTSWMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRK--PIPILACKTCQ-SNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGI 566
            L+     ++      + RC N   G   + DE G +C R  V  NGCC+ +   I   +C+TC  S  CC +YEYCVSCC++   + L + +L+  +   K+++++V   FE C+ KCRT+S SV HEN YR+   K+C G+
Sbjct:   59 LLAPEEQLNRTRGDPEARCRNSVQGRQWLADERGFVCRREEVLTNGCCNLELPGIGYYSCRTCNTSTHCCGVYEYCVSCCLHPGQQPLLERVLQAPN-TPKYIFASVTDHFELCLVKCRTNSHSVEHENKYRDPAAKHCYGL 199
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Zebrafish
Match: C5H12orf49 (zgc:110063 [Source:NCBI gene;Acc:553772])

HSP 1 Score: 107.071 bits (266), Expect = 1.892e-28
Identity = 55/131 (41.98%), Postives = 79/131 (60.31%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCD--RKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            ++C N   G  L+ DE+G +C R  +  NGCC+          CK+C +N CC+IYEYCVSCC+    + L +  L +++   + +++ V   FE C+AKCRTSS SV HEN+YRN   KYC G + P L+
Sbjct:   67 SQCRNSIQGKSLLTDELGYVCERKDLLVNGCCNVNAPSSSQYVCKSCLANGCCSIYEYCVSCCLQPDKQPLLERFLNRAAEGFQNLFTAVEDHFELCLAKCRTSSQSVQHENTYRNPQAKYCYGESPPELL 197
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Zebrafish
Match: C5H12orf49 (zgc:110063 [Source:NCBI gene;Acc:553772])

HSP 1 Score: 81.6481 bits (200), Expect = 6.717e-19
Identity = 41/105 (39.05%), Postives = 62/105 (59.05%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCD--RKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSS 506
            ++C N   G  L+ DE+G +C R  +  NGCC+          CK+C +N CC+IYEYCVSCC+    + L +  L +++   + +++ V   FE C+AKCRTSS
Sbjct:   71 SQCRNSIQGKSLLTDELGYVCERKDLLVNGCCNVNAPSSSQYVCKSCLANGCCSIYEYCVSCCLQPDKQPLLERFLNRAAEGFQNLFTAVEDHFELCLAKCRTSS 175
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Xenopus
Match: pak2 (p21 protein (Cdc42/Rac)-activated kinase 2 [Source:Xenbase;Acc:XB-GENE-966302])

HSP 1 Score: 119.013 bits (297), Expect = 6.730e-33
Identity = 58/131 (44.27%), Postives = 84/131 (64.12%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCD--RKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            N+C N   G +L+ D++G IC R  +  NGCC+       + +C+TC SN CC++YEYCVSCC+    + L +  L ++++A + ++  V   FE C+AKCRTSS SV HEN+YRN I KYC G + P L+
Sbjct:   72 NQCRNSVQGKLLITDDMGYICERKELLANGCCNINVASTKLYSCETCLSNGCCSVYEYCVSCCLQPNKQLLLERFLNRAAVAFQNLFQAVEDHFELCLAKCRTSSQSVQHENTYRNPIAKYCYGESPPELL 202
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Mouse
Match: 2410131K14Rik (RIKEN cDNA 2410131K14 gene [Source:MGI Symbol;Acc:MGI:1924042])

HSP 1 Score: 110.153 bits (274), Expect = 1.720e-29
Identity = 57/131 (43.51%), Postives = 78/131 (59.54%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILA---CKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPIL 581
            N+C N   G  L+ DE+G +C R  +  NGCCD   +P      C  C +N CC  YEYCVSCC+    + L +  L ++++A + ++  V   FE C+AKCRTSS SV HEN+YR+ I KYC G + P L
Sbjct:   73 NQCRNSVQGKHLLTDELGYVCERKDLLANGCCDVS-VPSTKQYCCDGCLANGCCEAYEYCVSCCLQPSKQLLLERFLNRAAVAFQNLFMAVEDHFELCLAKCRTSSQSVQHENTYRDPIAKYCYGESPPEL 202
[Close]
BLAST of UPF0454 protein C12orf49-like vs. UniProt/SwissProt
Match: sp|Q6GNT2|CL049_XENLA (UPF0454 protein C12orf49 homolog OS=Xenopus laevis OX=8355 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 4.293e-30
Identity = 56/131 (42.75%), Postives = 82/131 (62.60%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCD--RKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            N+C N   G +LV D++G IC R  +  NGCC+       + +C+TC  N CC++YE+CVSCC+    +   +  L K+++A + ++  V   FE C+AKCRTSS SV HEN+YRN I K+C G + P L+
Sbjct:   73 NQCRNSVQGKLLVTDDMGYICERKELLANGCCNINVASTKLYSCETCLPNGCCSVYEFCVSCCLQPNKQLQLERFLNKAAVAFQNLFQAVEDHFELCLAKCRTSSQSVQHENTYRNPIAKHCYGESPPELL 203
[Close]
BLAST of UPF0454 protein C12orf49-like vs. UniProt/SwissProt
Match: sp|Q5ZJE4|CL049_CHICK (UPF0454 protein C12orf49 homolog OS=Gallus gallus OX=9031 GN=RCJMB04_18o22 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 6.584e-30
Identity = 57/132 (43.18%), Postives = 83/132 (62.88%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIP---ILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            N+C N   G +L+ DE+G IC R  +  NGCC+   +P   + +C +C  N CC++YEYCVSCC+    + L +  L ++++A + ++  V   FE C+AKCRTSS SV HEN+YR+ I KYC G   P L+
Sbjct:   73 NQCRNSVQGKLLITDELGYICERKDLLVNGCCNVN-VPSTKLYSCDSCLPNGCCSVYEYCVSCCLQPSKQHLLERFLNRAAMAFQNLFMAVEDRFELCLAKCRTSSQSVQHENTYRDPIAKYCYGEYPPELL 203
[Close]
BLAST of UPF0454 protein C12orf49-like vs. UniProt/SwissProt
Match: sp|Q8BTG6|CL049_MOUSE (UPF0454 protein C12orf49 homolog OS=Mus musculus OX=10090 PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.203e-28
Identity = 57/131 (43.51%), Postives = 78/131 (59.54%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILA---CKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPIL 581
            N+C N   G  L+ DE+G +C R  +  NGCCD   +P      C  C +N CC  YEYCVSCC+    + L +  L ++++A + ++  V   FE C+AKCRTSS SV HEN+YR+ I KYC G + P L
Sbjct:   73 NQCRNSVQGKHLLTDELGYVCERKDLLANGCCDVS-VPSTKQYCCDGCLANGCCEAYEYCVSCCLQPSKQLLLERFLNRAAVAFQNLFMAVEDHFELCLAKCRTSSQSVQHENTYRDPIAKYCYGESPPEL 202
[Close]
BLAST of UPF0454 protein C12orf49-like vs. UniProt/SwissProt
Match: sp|Q503V3|CL049_DANRE (UPF0454 protein C12orf49 homolog OS=Danio rerio OX=7955 GN=zgc:110063 PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 6.021e-28
Identity = 56/131 (42.75%), Postives = 79/131 (60.31%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCD--RKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            ++C N   G  L+ DE+G +C R  +  NGCC+          CK+C SN CC+IYEYCVSCC+    + L +  L +++   + +++ V   FE C+AKCRTSS SV HEN+YRN   KYC G + P L+
Sbjct:   67 SQCRNSIQGKSLLTDELGYVCERKDLLVNGCCNVNAPSSSQYVCKSCLSNGCCSIYEYCVSCCLQPDKQPLLERFLNRAAEGFQNLFTAVEDHFELCLAKCRTSSQSVQHENTYRNPQAKYCYGESPPELL 197
[Close]
BLAST of UPF0454 protein C12orf49-like vs. UniProt/SwissProt
Match: sp|Q9H741|CL049_HUMAN (UPF0454 protein C12orf49 OS=Homo sapiens OX=9606 GN=C12orf49 PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 3.313e-27
Identity = 56/131 (42.75%), Postives = 78/131 (59.54%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILA---CKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPIL 581
            N+C N   G  L+ DE+G +C R  +  NGCC+   +P      C  C  N CC+ YEYCVSCC+    + L +  L ++++A + ++  V   FE C+AKCRTSS SV HEN+YR+ I KYC G + P L
Sbjct:   73 NQCRNSIQGKHLITDELGYVCERKDLLVNGCCNVN-VPSTKQYCCDGCWPNGCCSAYEYCVSCCLQPNKQLLLERFLNRAAVAFQNLFMAVEDHFELCLAKCRTSSQSVQHENTYRDPIAKYCYGESPPEL 202
[Close]
BLAST of UPF0454 protein C12orf49-like vs. TrEMBL
Match: A0A2B4SFZ1 (UPF0454 protein C12orf49-like OS=Stylophora pistillata OX=50429 GN=AWC38_SpisGene7946 PE=4 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 3.311e-31
Identity = 64/156 (41.03%), Postives = 94/156 (60.26%), Query Frame = 3 
Query:  123 KMNYSNKDLLILNTS---WMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDR--KPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRP 575
            + +Y + D+L + T+   W++  N  +   C N   GA+L+VDE G +C    +S +GCC    +      C+ CQ+N CC+IYE+CVSCC+N  NK L + IL   S     ++ +V   FE C+ KCRTSS SV+HENSY++K FK+C G+  P
Sbjct:   28 RKSYQDSDVLEVPTTEFQWINIENLTEFRDCRNSIQGALLIVDERGFVCNHKDLSASGCCHSGGESTKRYECRYCQNNNCCSIYEHCVSCCLNPDNKRLLEQILILGSSVPNVIFKSVSDQFELCLLKCRTSSKSVWHENSYKDKTFKHCFGLTGP 183
[Close]
BLAST of UPF0454 protein C12orf49-like vs. TrEMBL
Match: A0A093Q3V7 (UPF0454 protein C12orf49 (Fragment) OS=Manacus vitellinus OX=328815 GN=N305_12072 PE=4 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 9.667e-30
Identity = 59/132 (44.70%), Postives = 84/132 (63.64%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIP---ILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            N+C N   G +L+ DE+G IC R  +  NGCCD   +P   + +C++C  N CC+IYEYCVSCC+    + L +  L ++++A + ++  V   FE C+AKCRTSS SV HEN+YR+ I KYC G   P L+
Sbjct:   37 NQCRNSVQGKLLITDELGYICERKDLLVNGCCDVH-VPSTKLFSCESCLPNGCCSIYEYCVSCCLQPSKQHLLERFLNRAAIAFQNLFMAVEDHFELCLAKCRTSSQSVQHENTYRDPIAKYCYGEYPPELL 167
[Close]
BLAST of UPF0454 protein C12orf49-like vs. TrEMBL
Match: B2GUI5 (P21 protein (Cdc42/Rac)-activated kinase 2 OS=Xenopus tropicalis OX=8364 GN=whrn PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.057e-29
Identity = 58/131 (44.27%), Postives = 84/131 (64.12%), Query Frame = 3 
Query:  198 NRCSNFNSGAILVVDEIGRICLRSSVSGNGCCD--RKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            N+C N   G +L+ D++G IC R  +  NGCC+       + +C+TC SN CC++YEYCVSCC+    + L +  L ++++A + ++  V   FE C+AKCRTSS SV HEN+YRN I KYC G + P L+
Sbjct:   72 NQCRNSVQGKLLITDDMGYICERKELLANGCCNINVASTKLYSCETCLSNGCCSVYEYCVSCCLQPNKQLLLERFLNRAAVAFQNLFQAVEDHFELCLAKCRTSSQSVQHENTYRNPIAKYCYGESPPELL 202
[Close]
BLAST of UPF0454 protein C12orf49-like vs. TrEMBL
Match: A0A068YKS4 (Uncharacterized protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001048200 PE=4 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 2.764e-29
Identity = 64/163 (39.26%), Postives = 93/163 (57.06%), Query Frame = 3 
Query:  117 LEKMNYSNKDLL--ILNTSWMDAVNE----IKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDR--KPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPIL 581
            L K+ YS+      I N SW+ ++N     + +  C +   G   V DE G +C+RS++   GCCDR  +      C TC S +CC++YEYCVSCC+N  N  +WK +LE + L  +    +  S FEFC A+CRTSS SV + N+YR+   ++C G+  P L
Sbjct:    4 LSKIPYSSPPFPKPIYNFSWITSMNSSLVLLPKEHCHHTVQGVSRVTDEFGYVCIRSNLLRTGCCDRTKRTSSRFICSTCSSLRCCSVYEYCVSCCLNPVNLGIWKRVLELAQLKAQLDVLSATSVFEFCSARCRTSSESVVNANAYRDAERRFCFGVELPEL 166
[Close]
BLAST of UPF0454 protein C12orf49-like vs. TrEMBL
Match: A0A3M6U0R1 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00012925 PE=4 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 2.988e-29
Identity = 60/138 (43.48%), Postives = 84/138 (60.87%), Query Frame = 3 
Query:  168 WMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDR--KPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRP 575
            W++  N  +   C N   GA+L+VDE G +C R  +S +GCC    +      C+ CQ+N CC+IYE+CVSCC+N  N+ L + IL   S     +  +V   FE C+ KCRTSS SV+HENSY++K FK+C G+  P
Sbjct:   46 WINIENLTEFRDCRNSIQGALLIVDERGFVCNRKDLSASGCCHSGGESTKRYECRYCQNNNCCSIYEHCVSCCLNPDNRRLLEQILILGSSVPNVISKSVGDQFELCLVKCRTSSKSVWHENSYKDKTFKHCFGLTGP 183
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Cavefish
Match: zgc:110063 (chromosome unknown C12orf49 homolog [Source:NCBI gene;Acc:103033454])

HSP 1 Score: 106.686 bits (265), Expect = 2.457e-28
Identity = 56/134 (41.79%), Postives = 80/134 (59.70%), Query Frame = 3 
Query:  201 RCSNFNSGAILVVDEIGRICLRSSVSGNGCCD------RKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            +C N   G  L+ DE+G +C R  +  NGCC+      R+ I    CK+C  N CC++YEYCVSCC+    + L +  L +++   + +++ V   FE C+AKCRTSS SV HEN+YRN   KYC G + P L+
Sbjct:   68 QCRNSIQGKALLTDELGYVCERKDLLVNGCCNVNSPSSRQYI----CKSCLPNGCCSVYEYCVSCCLQPDKQPLLERFLNRAAEGFQNLFTAVEDHFELCLAKCRTSSQSVQHENTYRNPQAKYCYGDSPPELL 197
[Close]
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Sea Lamprey
Match: zgc:110063 (chromosome 12 open reading frame 49 [Source:HGNC Symbol;Acc:HGNC:26128])

HSP 1 Score: 110.538 bits (275), Expect = 7.328e-31
Identity = 57/130 (43.85%), Postives = 81/130 (62.31%), Query Frame = 3 
Query:  201 RCSNFNSGAILVVDEIGRICLRSSVSGNGCC-DRKPIPI-LACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            RC N   G +L+ DE G +C R +V  +GCC D  P     +C+ CQ N CC+IYE+CVSCC+  + + + +  L  ++ A + ++  VR  FE C+AKCRTSS SV HEN+YR+   KYC G + P L+
Sbjct:   43 RCRNSVQGRMLLTDERGFVCERRAVLPSGCCNDSAPGSAQYSCQGCQPNGCCSIYEHCVSCCLQPEQQAVLERFLGHAAAAFQNLFLAVRDHFELCLAKCRTSSQSVQHENTYRDPRAKYCYGEDPPELL 172
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Nematostella
Match: EDO39698 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9F9])

HSP 1 Score: 107.457 bits (267), Expect = 7.980e-30
Identity = 54/126 (42.86%), Postives = 76/126 (60.32%), Query Frame = 3 
Query:  204 CSNFNSGAILVVDEIGRICLRSSVSGNGCC--DRKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRP 575
            C N   G  L+VDE G +C R  +S +GCC  D +      C++CQ N CC+IYE CVSCC++ K K L + +L     A   +  ++   FE C+ KCRTSS SV+HENSY++  +K+C G+  P
Sbjct:    2 CRNSVQGPSLIVDERGYLCSRKDLSASGCCHSDGETTHRYNCESCQVNNCCSIYENCVSCCLDPKQKELLREVLNVWRTAPNVILKSITDQFELCLTKCRTSSKSVWHENSYKDNKYKHCFGLTSP 127
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Medaka
Match: zgc:110063 (chromosome 9 C12orf49 homolog [Source:NCBI gene;Acc:101164064])

HSP 1 Score: 106.301 bits (264), Expect = 3.314e-28
Identity = 56/137 (40.88%), Postives = 82/137 (59.85%), Query Frame = 3 
Query:  192 KQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCD------RKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILV 584
            +  +C N   G  L+ DE+G +C R  +  NGCC+      R+ I    CK+C +N CC+IYEYCVSCC+    + L +  L +++   + +++ V   FE C+AKCRTSS SV HEN+YRN   K+C G + P L+
Sbjct:   67 QMTQCRNSIQGKSLLTDELGYVCERKDLLVNGCCNVNAPNSRQHI----CKSCLANGCCSIYEYCVSCCLQPDKQLLLERFLNRAAEGFQNLFTAVEDHFELCLAKCRTSSQSVQHENTYRNPQAKFCYGESPPELL 199
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BLAST of UPF0454 protein C12orf49-like vs. Planmine SMEST
Match: SMESG000061283.1 (SMESG000061283.1)

HSP 1 Score: 319.701 bits (818), Expect = 7.408e-113
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 3 
Query:  126 MNYSNKDLLILNTSWMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILVKDVH 596
            MNYSNKDLLILNTSWMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILVKDVH
Sbjct:    1 MNYSNKDLLILNTSWMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILACKTCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFEFCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILVKDVH 157
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BLAST of UPF0454 protein C12orf49-like vs. Planmine SMEST
Match: SMESG000061283.1 (SMESG000061283.1)

HSP 1 Score: 186.037 bits (471), Expect = 1.155e-60
Identity = 90/90 (100.00%), Postives = 90/90 (100.00%), Query Frame = 3 
Query:  126 MNYSNKDLLILNTSWMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILACKTCQSNKCCAIYEYCVSCCVNEKN 395
            MNYSNKDLLILNTSWMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILACKTCQSNKCCAIYEYCVSCCVNEKN
Sbjct:    1 MNYSNKDLLILNTSWMDAVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILACKTCQSNKCCAIYEYCVSCCVNEKN 90

HSP 2 Score: 45.4394 bits (106), Expect = 2.173e-6
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 2 
Query:  398 NSLETDIREILTRRKVCLFHS 460
            NSLETDIREILTRRKVCLFHS
Sbjct:   93 NSLETDIREILTRRKVCLFHS 113
The following BLAST results are available for this feature:
BLAST of UPF0454 protein C12orf49-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
C12orf496.899e-2842.75chromosome 12 open reading frame 49 [Source:HGNC S... [more]
C12orf492.723e-860.98chromosome 12 open reading frame 49 [Source:HGNC S... [more]
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG156432.303e-2536.62gene:FBgn0030654 transcript:FBtr0074072[more]
CG156432.303e-2536.62gene:FBgn0030654 transcript:FBtr0340349[more]
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
C5H12orf491.892e-2841.98zgc:110063 [Source:NCBI gene;Acc:553772][more]
C5H12orf496.717e-1939.05zgc:110063 [Source:NCBI gene;Acc:553772][more]
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pak26.730e-3344.27p21 protein (Cdc42/Rac)-activated kinase 2 [Source... [more]
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
2410131K14Rik1.720e-2943.51RIKEN cDNA 2410131K14 gene [Source:MGI Symbol;Acc:... [more]
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BLAST of UPF0454 protein C12orf49-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6GNT2|CL049_XENLA4.293e-3042.75UPF0454 protein C12orf49 homolog OS=Xenopus laevis... [more]
sp|Q5ZJE4|CL049_CHICK6.584e-3043.18UPF0454 protein C12orf49 homolog OS=Gallus gallus ... [more]
sp|Q8BTG6|CL049_MOUSE1.203e-2843.51UPF0454 protein C12orf49 homolog OS=Mus musculus O... [more]
sp|Q503V3|CL049_DANRE6.021e-2842.75UPF0454 protein C12orf49 homolog OS=Danio rerio OX... [more]
sp|Q9H741|CL049_HUMAN3.313e-2742.75UPF0454 protein C12orf49 OS=Homo sapiens OX=9606 G... [more]
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BLAST of UPF0454 protein C12orf49-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2B4SFZ13.311e-3141.03UPF0454 protein C12orf49-like OS=Stylophora pistil... [more]
A0A093Q3V79.667e-3044.70UPF0454 protein C12orf49 (Fragment) OS=Manacus vit... [more]
B2GUI51.057e-2944.27P21 protein (Cdc42/Rac)-activated kinase 2 OS=Xeno... [more]
A0A068YKS42.764e-2939.26Uncharacterized protein OS=Echinococcus multilocul... [more]
A0A3M6U0R12.988e-2943.48Uncharacterized protein OS=Pocillopora damicornis ... [more]
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1100632.457e-2841.79chromosome unknown C12orf49 homolog [Source:NCBI g... [more]
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1100637.328e-3143.85chromosome 12 open reading frame 49 [Source:HGNC S... [more]
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO396987.980e-3042.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of UPF0454 protein C12orf49-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1100633.314e-2840.88chromosome 9 C12orf49 homolog [Source:NCBI gene;Ac... [more]
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BLAST of UPF0454 protein C12orf49-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000061283.17.408e-113100.00SMESG000061283.1[more]
SMESG000061283.11.155e-60100.00SMESG000061283.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010070 ID=SMED30010070|Name=UPF0454 protein C12orf49-like|organism=Schmidtea mediterranea sexual|type=transcript|length=673bp
GCGACTATGATTAACTGTGTAATAAAATGCATATAAATTTTTTATTAAAA
TTTTTAAGATCATTACTTTTTCTTTTAATATGTTTATGTCTATTCATATT
TTCAATAATATATAAATTAGAAAAGATGAACTACTCTAACAAGGATTTAC
TTATATTAAATACGTCGTGGATGGACGCAGTTAATGAAATTAAACAAAAC
CGATGCTCAAATTTCAACAGTGGTGCTATATTAGTTGTGGATGAAATCGG
TAGGATTTGCTTGAGGAGTTCCGTGAGCGGAAACGGCTGTTGTGACCGCA
AACCAATCCCGATCCTGGCCTGTAAAACTTGTCAATCGAATAAGTGCTGT
GCAATTTATGAGTATTGTGTCTCGTGTTGCGTTAATGAGAAAAATAAAAC
TCTCTGGAAACTGATATTAGAGAAATCCTCACTCGCAGAAAAGTTTGTCT
ATTCCACAGTTAGATCTCCATTTGAATTTTGTGTTGCTAAATGTCGGACT
TCCTCTGCGAGTGTTTTCCATGAAAATTCTTACCGGAATAAGATTTTCAA
ATATTGCTGTGGAATCAATCGGCCGATTCTGGTGAAAGATGTTCATTAAT
TAGACTGTTTCTTTTCTGTTTGAAATTTGTGTTTTTGTTAATAGATTTTT
AATTGAAATTCGAATCAAGGCGC
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protein sequence of SMED30010070-orf-1

>SMED30010070-orf-1 ID=SMED30010070-orf-1|Name=SMED30010070-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=191bp
MHINFLLKFLRSLLFLLICLCLFIFSIIYKLEKMNYSNKDLLILNTSWMD
AVNEIKQNRCSNFNSGAILVVDEIGRICLRSSVSGNGCCDRKPIPILACK
TCQSNKCCAIYEYCVSCCVNEKNKTLWKLILEKSSLAEKFVYSTVRSPFE
FCVAKCRTSSASVFHENSYRNKIFKYCCGINRPILVKDVH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005576extracellular region
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR019352UPF0454
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019352Uncharacterised protein family UPF0454PFAMPF10218DUF2054coord: 57..177
e-value: 4.4E-32
score: 110.9
IPR019352Uncharacterised protein family UPF0454PANTHERPTHR13481UNCHARACTERIZEDcoord: 13..182
NoneNo IPR availableTMHMMTMhelixcoord: 7..29