SMED30010048

Overview
NameSMED30010048
Smed IDSMED30010048
Length (bp)1635
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010048 (SMED30010048) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010048 (SMED30010048) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010048 (SMED30010048) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010048 (SMED30010048) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010048

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30010048SMESG000000602.1 SMESG000000601.1 dd_Smed_v4_7042_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010048SMESG000000602.1 SMESG000000601.1 dd_Smed_v4_7042_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010048SMESG000000602.1 SMESG000000601.1 dd_Smed_v4_7042_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010048 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.013264:787..15698 -4762
Homology
[Close]
BLAST of SMED30010048 vs. TrEMBL
Match: K1PUR8 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10001639 PE=4 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 9.648e-50
Identity = 136/449 (30.29%), Postives = 228/449 (50.78%), Query Frame = 1 
Query:  241 SSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVTLVAFSYSYQILTCRFVMS-----------------PEDKITTP---PPENVTSKMATLVNKTNIME---------CSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGLNMLLNNEMFQINKEMVLAFRIYFVIHLMPLLIVPLIQCARVNKATECFKEWALHMSVFGYRKEKRKELDSFLLYAIGYSSKAKLLSVPLQWPHIFIVCSFGGLILLSIIQTGI 1500
            ++TTLR       C+K+++ P+  +LM +GWR  G +    ++ +    I N +    +V L+ ++Y Y+I+ C++ +                   E  +T         VT   A L N T + +         C HI+TTYVIP+ +HF+AY++GI+H+R ++NE +Y +MEK    +T       +Q K+  +L I MA   IWV ++L    +Y+ AF+     F+        K+  ++   LF +++ G L+++ VT  VI NYV +CE+V FY+  ++ +++ +  +L     DI  I   + L+N     MT+LI      L I G+ +L+ N+   +    V  +R +F +  + +L  PL Q ARVN  +   K+ AL M VFGY+   + ELDSF+L+      KAKL  VP+   ++  +   G  ILL + QT +
Sbjct:   75 TTTTLRR------CKKQVLRPYWRLLMFIGWRGFGRE--SVNSGRMRWTILNIVYPVFIVCLLLYTYIYEIVACQWKLDLHKDTQVITTTTPTPVSTESNVTDGVSIDEYQVTPSYALLQNLTTMRKIGPSEPPEACQHIITTYVIPNVLHFLAYIMGIVHFRIQENEQLYAIMEKVFLQATPSYSRSASQQKMIKKLRILMAVGAIWVAVTLVMQGLYEYAFNFPQLLFF-------RKVGTTVHWVLFSIELLGILVLNSVTLAVIANYVTHCEMVLFYLKGLALRLQEKSTELKAAMKDILFIKQNVSLLNGPVARMTALISVIFAELTIIGIVILILNKNDSVK---VWIYRSFFPLVWLFILCAPLYQAARVNSVSNRMKKIALEMRVFGYKNSSQLELDSFILFISQARMKAKLFHVPILPQYLIAILVLGAFILLILFQTSV 505
[Close]
BLAST of SMED30010048 vs. TrEMBL
Match: A0A210R2X5 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT20919 PE=4 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 2.085e-45
Identity = 124/440 (28.18%), Postives = 222/440 (50.45%), Query Frame = 1 
Query:  271 MKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGV-LVTLVAFSYSYQILTCRFVMS------------------PEDKITTPPPENVTSKMATLVNKTNIME-----------CSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGLNMLLNNEMFQINKEMVLAFRIYFVIHLMPLLIVPLIQCARVNKATECFKEWALHMSVFGYRKEKRKELDSFLLYAIGYSSKAKLLSVPLQWPHIFIVCSFGGLILLSIIQTGI 1500
            +K+C+++++ P+  +LM +GWR  G +  +      F   + NIL  V +V L+ ++Y Y+++ C++ ++                  P    T P    +T   A  +   N+ +           C HI+TTYVIP+ +HF+AY++G+ H+R ++NE +Y LMEK    +T +     +Q K+  +L +++A    WV ++L    +Y+ AFD  +  F+        K        LFI+++ G ++++ V+  VI NYV  CE+  FY+  ++ +++ +  ++     D+ ++   + L+N     MTSL+      L I G+++L+ N+    N   V  +R  F +    +LI PL Q A+VN      K+ A+ M VFGY+   + ELDSFL +      KAK+ S+P+   ++  +      ILL + QT I
Sbjct:   99 LKLCKRQVLRPYWRLLMFIGWRGFGRESVNSGHRGWF---YLNILYPVFIVILLLYTYVYEVVACQWKLNIHKDTQFVPTTTMLPSTIPASNTTPPFINELTFSPAESIMTLNLSKLHHAHAQPPEACEHIITTYVIPNVLHFIAYIMGMYHFRIQENEQLYALMEKVFLQATPLYNRSISQQKMIRKLRLFLAVGGFWVGVTLGMQGLYEWAFDFPALAFF-------RKTGKVAHWVLFIVELLGIMVLNSVSLAVIANYVTQCEMTLFYIRGLALRLQEKSTEIKAAMKDLFVVKQNLSLLNGPIARMTSLVAVIFAELIIIGISILVLNKN---NSPKVWTYRSIFPLVWASMLICPLYQAAKVNSVCNGIKKIAVEMRVFGYKGSSQLELDSFLGFVSQVQLKAKIFSIPVMPSYLISILVLTSFILLILFQTSI 525
[Close]
BLAST of SMED30010048 vs. TrEMBL
Match: A0A2C9KHL1 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106055683 PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.574e-42
Identity = 124/440 (28.18%), Postives = 212/440 (48.18%), Query Frame = 1 
Query:  259 TLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVTLVAFSYSYQILTCRFVM--------------SPEDKITTPPPENVTSKMA-------------TLVNKTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGLNMLLNNEMFQINKEMVLAFRIYFVIHLMPLLIVPLIQCARVNKATECFKEWALHMSVFGYRKEKRKELDSFLLYAIGYSSKAKLLSVPLQWPHIFIVCSFGGLILLSIIQTG 1497
            T   ++ C++ ++ P+  +L+ +GWR  G      D ++R     N +   V+V L+ ++Y Y+IL C + +              +P    TT    ++T+ M+             +L    NI  C HIVTTY IP+ +HF+AY LG+ ++R ++NE +Y LMEK    +T ++    +Q K+  +L +++    IWV  ++    +Y  AF+    P +         I  S    LF +++ G  + + V   +++NY   CE++KFYV  +  +++ +   L     D+ ++  ++ L+N     MTSL       L I G+ +L  NE    +   V A+R +F +    +L  PL+Q ARVN      ++ +L M VFGY+     +LDSFL +      KAKL  VP+   H+         ILL ++QT 
Sbjct:   99 TTNTLRKCKQLVLRPYWGLLLFIGWR--GFHRESIDASKRRWRCINFMYPAVIVLLLLYTYMYEILACEWKLDIKTDSKFMEPTSSTPVTTRTTKNHSHITAPMSFIQSAHPLGNNLSSLSISQNI-GCQHIVTTYFIPNLLHFLAYCLGLYYFRIQENEQLYALMEKVFLQATPLQARTASQQKMIYKLRLFLILGAIWVFFTVLLQCLYVWAFNFPKLPLFET-------IGESGYWVLFSVELMGRTVFNSVILAIVMNYATQCEMIKFYVRGLGLRLQEKSTDLKTAMKDLLMLRQSLSLLNGIISKMTSLAAIILAELTIIGVCILFLNEY---DDAKVWAYRSFFPVIWALMLCFPLVQAARVNSICLRIQKISLEMRVFGYKGASILDLDSFLQFVSLTKLKAKLFHVPMLPSHLIFTAIATSFILLILVQTS 525
[Close]
BLAST of SMED30010048 vs. TrEMBL
Match: A0A2C9KHM1 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106055683 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 2.067e-42
Identity = 124/440 (28.18%), Postives = 212/440 (48.18%), Query Frame = 1 
Query:  259 TLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVTLVAFSYSYQILTCRFVM--------------SPEDKITTPPPENVTSKMA-------------TLVNKTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGLNMLLNNEMFQINKEMVLAFRIYFVIHLMPLLIVPLIQCARVNKATECFKEWALHMSVFGYRKEKRKELDSFLLYAIGYSSKAKLLSVPLQWPHIFIVCSFGGLILLSIIQTG 1497
            T   ++ C++ ++ P+  +L+ +GWR  G      D ++R     N +   V+V L+ ++Y Y+IL C + +              +P    TT    ++T+ M+             +L    NI  C HIVTTY IP+ +HF+AY LG+ ++R ++NE +Y LMEK    +T ++    +Q K+  +L +++    IWV  ++    +Y  AF+    P +         I  S    LF +++ G  + + V   +++NY   CE++KFYV  +  +++ +   L     D+ ++  ++ L+N     MTSL       L I G+ +L  NE    +   V A+R +F +    +L  PL+Q ARVN      ++ +L M VFGY+     +LDSFL +      KAKL  VP+   H+         ILL ++QT 
Sbjct:   96 TTNTLRKCKQLVLRPYWGLLLFIGWR--GFHRESIDASKRRWRCINFMYPAVIVLLLLYTYMYEILACEWKLDIKTDSKFMEPTSSTPVTTRTTKNHSHITAPMSFIQSAHPLGNNLSSLSISQNI-GCQHIVTTYFIPNLLHFLAYCLGLYYFRIQENEQLYALMEKVFLQATPLQARTASQQKMIYKLRLFLILGAIWVFFTVLLQCLYVWAFNFPKLPLFET-------IGESGYWVLFSVELMGRTVFNSVILAIVMNYATQCEMIKFYVRGLGLRLQEKSTDLKTAMKDLLMLRQSLSLLNGIISKMTSLAAIILAELTIIGVCILFLNEY---DDAKVWAYRSFFPVIWALMLCFPLVQAARVNSICLRIQKISLEMRVFGYKGASILDLDSFLQFVSLTKLKAKLFHVPMLPSHLIFTAIATSFILLILVQTS 522
[Close]
BLAST of SMED30010048 vs. TrEMBL
Match: A0A2C9KHP7 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106055683 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 2.438e-42
Identity = 124/440 (28.18%), Postives = 212/440 (48.18%), Query Frame = 1 
Query:  259 TLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVTLVAFSYSYQILTCRFVM--------------SPEDKITTPPPENVTSKMA-------------TLVNKTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGLNMLLNNEMFQINKEMVLAFRIYFVIHLMPLLIVPLIQCARVNKATECFKEWALHMSVFGYRKEKRKELDSFLLYAIGYSSKAKLLSVPLQWPHIFIVCSFGGLILLSIIQTG 1497
            T   ++ C++ ++ P+  +L+ +GWR  G      D ++R     N +   V+V L+ ++Y Y+IL C + +              +P    TT    ++T+ M+             +L    NI  C HIVTTY IP+ +HF+AY LG+ ++R ++NE +Y LMEK    +T ++    +Q K+  +L +++    IWV  ++    +Y  AF+    P +         I  S    LF +++ G  + + V   +++NY   CE++KFYV  +  +++ +   L     D+ ++  ++ L+N     MTSL       L I G+ +L  NE    +   V A+R +F +    +L  PL+Q ARVN      ++ +L M VFGY+     +LDSFL +      KAKL  VP+   H+         ILL ++QT 
Sbjct:  109 TTNTLRKCKQLVLRPYWGLLLFIGWR--GFHRESIDASKRRWRCINFMYPAVIVLLLLYTYMYEILACEWKLDIKTDSKFMEPTSSTPVTTRTTKNHSHITAPMSFIQSAHPLGNNLSSLSISQNI-GCQHIVTTYFIPNLLHFLAYCLGLYYFRIQENEQLYALMEKVFLQATPLQARTASQQKMIYKLRLFLILGAIWVFFTVLLQCLYVWAFNFPKLPLFET-------IGESGYWVLFSVELMGRTVFNSVILAIVMNYATQCEMIKFYVRGLGLRLQEKSTDLKTAMKDLLMLRQSLSLLNGIISKMTSLAAIILAELTIIGVCILFLNEY---DDAKVWAYRSFFPVIWALMLCFPLVQAARVNSICLRIQKISLEMRVFGYKGASILDLDSFLQFVSLTKLKAKLFHVPMLPSHLIFTAIATSFILLILVQTS 535
[Close]
BLAST of SMED30010048 vs. Ensembl Nematostella
Match: EDO40955 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5S9])

HSP 1 Score: 93.2041 bits (230), Expect = 3.242e-20
Identity = 92/397 (23.17%), Postives = 170/397 (42.82%), Query Frame = 1 
Query:  268 IMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVTLVAFSYSYQILTC--RFVMSP----------------EDKITTP----PPENVTSKMAT-------LVNKT----------------------------------NIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGLNMLLNNEMFQINKEMVLAFRIYFVIHLMPLLIVPLI 1269
            +++ C ++++ P+  ML ++GWRP+    +H    + FL + N     V+  L+ FSY   I  C  R  + P                   ITTP    P  +  S M T       L  KT                                     EC+HI++ YV+P F+HF+A+++G  H+R +++E ++ L+E     S       K Q K+  RL  ++ A  +W+   L  +I +  +F   S   +  H  +   + AS+     ++        + VT V++LNY   C+L+ +++  I+ ++E +  +L  +  D+  +   +  +NS +  +T+LI F    + + GL +L  N       ++V  +R    +  +  L+ PLI
Sbjct:   92 VLQKCNQQVMVPYFSMLSVIGWRPLCRDSFH---CRGFLYLVNIFYTLVIFLLLLFSYVASITACQARLDIEPLIPDTTSLTPTHRPTTSHNITTPHPTTPSNSTNSTMPTTQSIPSSLPPKTMPAPTVKHPTKSTQYPDVITFFTMYTMSPAQPDWKPTECTHIMSGYVVPKFLHFIAFVIGFYHFRIQESEGLHALVEMVFLQS-------KEQSKIVKRLRSFLLAGLVWLLGMLACYIFFCFSFGLASVTGF-RHSVSRQWVAASMMVLGDLVA-------NCVTFVIMLNYCAQCQLLIYFLRDIALRMEEKTAELGHLMKDLWEVKKHLTNLNSVSAHITTLIIFNFCEVAVIGLTILFRNSEGVPTSQVV--YRAIIPVMCLITLMCPLI 468
[Close]
BLAST of SMED30010048 vs. Planmine SMEST
Match: SMESG000000601.1 (SMESG000000601.1)

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 343/346 (99.13%), Postives = 345/346 (99.71%), Query Frame = 1 
Query:  124 MSQRKSFNSLRNPSSERNSKVDNLPNLXXXXXXXXXXXXSSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVNKTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGL 1161
            MSQRKSFNSLRNPSSERNS VDNLPNLDIHDNINEDIEISSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFN+ILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVNKTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASLGNFLFILDIFGFLLMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDIQIIGIAIDLINSTTGTMTSLIYFYTMTLFIGG+
Sbjct:    1 MSQRKSFNSLRNPSSERNSNVDNLPNLDIHDNINEDIEISSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNSILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVNKTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASLGNFLFILDIFGFLLMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDIQIIGIAIDLINSTTGTMTSLIYFYTMTLFIGGM 346
[Close]
BLAST of SMED30010048 vs. Planmine SMEST
Match: SMESG000000601.1 (SMESG000000601.1)

HSP 1 Score: 611.298 bits (1575), Expect = 0.000e+0
Identity = 343/348 (98.56%), Postives = 345/348 (99.14%), Query Frame = 1 
Query:  124 MSQRKSFNSLRNPSSERNSKVDNLPNLXXXXXXXXXXXXSSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVN--KTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGL 1161
            MSQRKSFNSLRNPSSERNS VDNLPNLDIHDNINEDIEISSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFN+ILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVN  KTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASLGNFLFILDIFGFLLMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDIQIIGIAIDLINSTTGTMTSLIYFYTMTLFIGG+
Sbjct:    1 MSQRKSFNSLRNPSSERNSNVDNLPNLDIHDNINEDIEISSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNSILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVNSTKTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASLGNFLFILDIFGFLLMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDIQIIGIAIDLINSTTGTMTSLIYFYTMTLFIGGM 348
[Close]
BLAST of SMED30010048 vs. Planmine SMEST
Match: SMESG000000601.1 (SMESG000000601.1)

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 342/348 (98.28%), Postives = 345/348 (99.14%), Query Frame = 1 
Query:  124 MSQRKSFNSLRNPSSERNSKVDNLPNLXXXXXXXXXXXXSSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVN--KTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASXXXXXXXXXXXXXXXMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDXXXXXXXXXXXNSTTGTMTSLIYFYTMTLFIGGL 1161
            M+QRKSFNSLRNPSSERNS VDNLPNLDIHDNINEDIEISSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFN+ILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVN  KTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASLGNFLFILDIFGFLLMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDIQIIGIAIDLINSTTGTMTSLIYFYTMTLFIGG+
Sbjct:    1 MNQRKSFNSLRNPSSERNSNVDNLPNLDIHDNINEDIEISSTTLRTLEIMKMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNSILLGVLVTLVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVNSTKTNIMECSHIVTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKTQYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENKINASLGNFLFILDIFGFLLMSIVTTVVILNYVLYCELVKFYVNAISKKIEMRHIKLNVVKNDIQIIGIAIDLINSTTGTMTSLIYFYTMTLFIGGM 348
The following BLAST results are available for this feature:
BLAST of SMED30010048 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1PUR89.648e-5030.29Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A210R2X52.085e-4528.18Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
A0A2C9KHL11.574e-4228.18Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A2C9KHM12.067e-4228.18Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A2C9KHP72.438e-4228.18Uncharacterized protein OS=Biomphalaria glabrata O... [more]
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BLAST of SMED30010048 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO409553.242e-2023.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30010048 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010048 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000000601.10.000e+099.13SMESG000000601.1[more]
SMESG000000601.10.000e+098.56SMESG000000601.1[more]
SMESG000000601.10.000e+098.28SMESG000000601.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010048 ID=SMED30010048|Name=SMED30010048|organism=Schmidtea mediterranea sexual|type=transcript|length=1635bp
CGTGACACTGCACTGTATTATTAATATTAACTAGGGAAAGACCTAGATCA
AAGTACGGAGTAAGTTGTATTAAACAAATGAAGTAGTTAATTTATTAAAA
TTATATTTTTATTAATCTCAAATATGAGTCAAAGAAAGTCATTTAATTCT
TTACGGAATCCGTCAAGCGAGAGAAATAGCAAGGTAGATAATTTACCTAA
CCTGGATATTCACGACAATATCAACGAGGACATTGAAATAAGTTCCACGA
CATTAAGAACATTAGAAATTATGAAAATGTGCCGAAAACGAATCATATAT
CCCTTTAATATTATGCTAATGATGGTAGGATGGCGTCCTGTTGGTTCGCA
AATGTATCATTACGATACCAATCAGAGATTCCTATGCATATTTAATAACA
TTCTATTGGGAGTCTTGGTTACGTTGGTGGCATTTTCATATTCATATCAA
ATCCTAACATGCAGATTTGTTATGAGTCCGGAAGATAAAATTACAACACC
CCCACCAGAAAATGTGACTTCGAAAATGGCTACATTAGTTAACAAAACGA
ATATTATGGAGTGTAGCCACATTGTAACAACATATGTGATTCCAAGTTTC
ATTCACTTCATGGCTTACTTGCTTGGAATACTCCATTATCGTTTTCGGAA
AAATGAAGATATTTATGGTCTCATGGAAAAGGCATTGCAATACTCTACAA
GCATGGAGCAGAACAAGAAAACCCAATATAAAGTCACTACACGTCTATCC
ATCTGGATGGCGGCTGCCTGTATATGGGTATGCTTATCATTGACAAATCA
CATCATGTATGATGTGGCTTTCGACACCTTTTCCAAGCCATTTTACAGCA
ATCATATCAACGCAGAAAATAAAATAAATGCGAGTCTCGGAAATTTTTTA
TTTATTTTAGATATATTTGGATTTCTACTAATGTCGATTGTCACAACTGT
TGTGATCCTTAATTATGTGCTCTATTGTGAGTTGGTGAAATTCTACGTCA
ACGCCATCAGCAAAAAGATTGAAATGAGGCATATCAAATTGAATGTTGTC
AAAAATGACATCCAGATCATTGGAATTGCGATAGACCTGATAAACTCCAC
CACCGGAACCATGACAAGCTTGATTTACTTTTACACAATGACATTATTTA
TTGGAGGATTGAATATGCTACTCAACAATGAAATGTTTCAAATCAACAAA
GAAATGGTGCTGGCATTCAGGATCTACTTTGTCATTCACTTAATGCCTTT
ACTAATAGTCCCTTTGATACAGTGTGCACGAGTCAACAAGGCCACTGAAT
GCTTCAAAGAATGGGCATTGCATATGTCAGTTTTTGGATATCGCAAAGAA
AAAAGGAAAGAATTAGACTCATTTTTACTTTACGCAATTGGATATAGTTC
AAAAGCTAAGCTGCTGTCAGTGCCATTGCAATGGCCCCATATATTTATTG
TTTGTAGTTTTGGCGGACTAATTTTATTATCCATTATACAAACGGGAATT
TGGTGCAACTTGCGATTCATCGGGAAATTGTAAAGAAATGTATTTTGAAT
GTATAAAAGATAAAACATATTTGAATTTTTATCGAAATTGTTAATATATT
TTCATTTTGTTCATTATAATTTCATTCGAAAAAAA
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protein sequence of SMED30010048-orf-1

>SMED30010048-orf-1 ID=SMED30010048-orf-1|Name=SMED30010048-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=470bp
MSQRKSFNSLRNPSSERNSKVDNLPNLDIHDNINEDIEISSTTLRTLEIM
KMCRKRIIYPFNIMLMMVGWRPVGSQMYHYDTNQRFLCIFNNILLGVLVT
LVAFSYSYQILTCRFVMSPEDKITTPPPENVTSKMATLVNKTNIMECSHI
VTTYVIPSFIHFMAYLLGILHYRFRKNEDIYGLMEKALQYSTSMEQNKKT
QYKVTTRLSIWMAAACIWVCLSLTNHIMYDVAFDTFSKPFYSNHINAENK
INASLGNFLFILDIFGFLLMSIVTTVVILNYVLYCELVKFYVNAISKKIE
MRHIKLNVVKNDIQIIGIAIDLINSTTGTMTSLIYFYTMTLFIGGLNMLL
NNEMFQINKEMVLAFRIYFVIHLMPLLIVPLIQCARVNKATECFKEWALH
MSVFGYRKEKRKELDSFLLYAIGYSSKAKLLSVPLQWPHIFIVCSFGGLI
LLSIIQTGIWCNLRFIGKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane