C2H2-type domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010020 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A443S1W5 (Uncharacterized protein (Fragment) OS=Leptotrombidium deliense OX=299467 GN=B4U80_06898 PE=4 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 4.663e-9 Identity = 43/98 (43.88%), Postives = 55/98 (56.12%), Query Frame = 1 Query: 454 EFVGVYPLDKIPYLDDNNA-IVVNTQSSNLGGEHWIAVYV-KPNKIHVFDPMGFYYPAL---LVSKLQTVGKSIV-YNKHRYQNPLTTTCGQHCLVWL 729 EFVG +P D IP A +VNT SSN GEHW+A+Y+ K NK FD G P L L + K+ V YN Q+PL+TTCG+ CL ++ Sbjct: 3 EFVGTFPRDNIPTTTRRPASFIVNTDSSNEPGEHWVAIYLTKNNKAEYFDSFGL--PPLHRDLTEFIHEHAKNGVKYNNICIQHPLSTTCGKFCLKYV 98
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A3C1S1Q5 (Uncharacterized protein OS=Tenacibaculum sp. OX=1906242 GN=DDE71_02150 PE=4 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 8.952e-9 Identity = 36/137 (26.28%), Postives = 72/137 (52.55%), Query Frame = 1 Query: 418 QVEKIGKQLLGNEFVGVYPLDKIPYLDDNNA--IVVNTQSSNLGGEHWIAVYVKPNKIHVFDPMG--FYYPALLVSKLQTVGKSIVYNKHRYQNPLTTTCGQHCLVWLNSMYKRIDXXXXXXXXXXYFIDISVFENE 816 ++ K KQ +F+GV+ +D++ + N ++ NT +S G+HW+A+ + +I FD + F Y ++ K +++N + Q+ ++ CG HCLV+ +M K + + + + F+D+S+ E E Sbjct: 5 EINKFAKQFGLKKFLGVFAVDELSLIPKNRLGLVIFNTDTSQSVGQHWVALCITKTRIFYFDSLNIKFQYSTHFTDFMKYTKKELIWNTIQIQSNISEKCGIHCLVFCYAMQKYKNKRGYKNFLKT-FLDLSIEERE 140
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A1I8H9W8 (Guanylate cyclase OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 66.6254 bits (161), Expect = 9.392e-8 Identity = 30/101 (29.70%), Postives = 61/101 (60.40%), Query Frame = 3 Query: 27 ETYTQDFDNDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLP-QNGNILLDIKFSKAVEEPVHVIIYGVFENEIQITANE 326 + +T F NY Y +++++G ++++L+ ++ D+ + Y FF + L PD +L +P + GN+ L IKFS A+ ++V+++G F++ +QIT + Sbjct: 1671 QPFTPRFGVHNYAREYAM-LLKSLGKMNRDLDIDVSYSDYGAGYCFFAWNLMPDMELGGHSMPREQGNLTLQIKFSAALTAGINVLVFGYFDSTVQITRGQ 1770
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A1I8I4M6 (CN hydrolase domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 66.6254 bits (161), Expect = 9.452e-8 Identity = 31/98 (31.63%), Postives = 60/98 (61.22%), Query Frame = 3 Query: 27 ETYTQDFDNDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLP-QNGNILLDIKFSKAVEEPVHVIIYGVFENEIQIT 317 + +T F NY Y +++++G ++++L+ ++ D+ + Y FF + L PD +L +P + GN+ L IKFS A+ ++V+++G F+N +QIT Sbjct: 1363 QPFTPRFGVHNYAREYAM-LLKSLGKMNRDLDIDVSYSDYGAGYCFFAWNLMPDMELGGHSMPRERGNLTLQIKFSAALAAGINVLVFGYFDNTVQIT 1459
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A354GG20 (Uncharacterized protein OS=Tenacibaculum sp. OX=1906242 GN=DDY16_03295 PE=4 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 1.290e-7 Identity = 30/114 (26.32%), Postives = 64/114 (56.14%), Query Frame = 1 Query: 418 QVEKIGKQLLGNEFVGVYPLDKIPYLDDNNA--IVVNTQSSNLGGEHWIAVYVKPNKIHVFDPM--GFYYPALLVSKLQTVGKSIVYNKHRYQNPLTTTCGQHCLVWLNSMYKR 747 ++ ++ ++L+ +F+GV+ +D++ + + ++ NT +S G+HWIA+ + N I+ FD + FY+ ++ + K +N + Q+ L+ CG H LV+ +M K+ Sbjct: 5 EINELAEKLVIKDFIGVFAVDELILIPKSRTGLLIFNTDTSQNIGQHWIALCITKNNIYYFDSLFCEFYHSKHFKEYMKFIKKKFTWNTIQIQHDLSDKCGIHSLVFCYAMRKK 118
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000050353.1 (SMESG000050353.1) HSP 1 Score: 207.608 bits (527), Expect = 6.762e-97 Identity = 95/108 (87.96%), Postives = 103/108 (95.37%), Query Frame = 3 Query: 3 LSKKSDYKETYTQDFDNDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGNILLDIKFSKAVEEPVHVIIYGVFENEIQITANE 326 LSK SDY+ETYTQDF+NDNYCATYMQSIVRNMGYLDKNLNCGITL+DFK+KYPFFTFVLAPDFDLNQSQLPQNGN+ LDIKF+KA EPVHV+IYGVFENE+QITAN Sbjct: 162 LSKNSDYRETYTQDFENDNYCATYMQSIVRNMGYLDKNLNCGITLDDFKNKYPFFTFVLAPDFDLNQSQLPQNGNLRLDIKFAKATSEPVHVVIYGVFENEVQITANR 269 HSP 2 Score: 166.392 bits (420), Expect = 6.762e-97 Identity = 75/102 (73.53%), Postives = 88/102 (86.27%), Query Frame = 1 Query: 421 VEKIGKQLLGNEFVGVYPLDKIPYLDDNNAIVVNTQSSNLGGEHWIAVYVKPNKIHVFDPMGFYYPALLVSKLQTVGKSIVYNKHRYQNPLTTTCGQHCLVW 726 V +IGKQ+LG+EF+GV+PLDKIPYL +N AI+VNTQSS+LGGEHW+A+Y+KP KI VFDPMGFYYPAL+VSKLQ +GK IVYNK YQ T TCGQHCL W Sbjct: 273 VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPCKIFVFDPMGFYYPALVVSKLQAMGKPIVYNKTMYQKQWTLTCGQHCLAW 374
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000054630.1 (SMESG000054630.1) HSP 1 Score: 172.17 bits (435), Expect = 5.340e-87 Identity = 72/109 (66.06%), Postives = 93/109 (85.32%), Query Frame = 1 Query: 421 VEKIGKQLLGNEFVGVYPLDKIPYLDDNNAIVVNTQSSNLGGEHWIAVYVKPNKIHVFDPMGFYYPALLVSKLQTVGKSIVYNKHRYQNPLTTTCGQHCLVWLNSMYKR 747 ++ I K+L+G F+GV+PLDKIPY++ N A++VNTQS+NLGGEHW+A+YVKP+KIHVFDPMGFYYP LL+SKL+++ I YN+ RYQNPLT TCGQHCL WL SM+ + Sbjct: 1297 IQTIAKKLIGKNFIGVFPLDKIPYININKALIVNTQSANLGGEHWLAIYVKPDKIHVFDPMGFYYPPLLISKLESMLMPIEYNRIRYQNPLTKTCGQHCLAWLKSMFSK 1405 HSP 2 Score: 169.088 bits (427), Expect = 5.340e-87 Identity = 76/108 (70.37%), Postives = 93/108 (86.11%), Query Frame = 3 Query: 3 LSKKSDYKETYTQDFDNDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGNILLDIKFSKAVEEPVHVIIYGVFENEIQITANE 326 + ++DY+E Y+QDF NDNY ++YMQSIVRNMGYL+KN+NCGITL DFK+KYPFFTFVLAPDFDLNQSQLPQ+GN+ LDIKF + E V V++YGVFE EIQIT++ Sbjct: 1188 MEDRNDYREKYSQDFANDNYSSSYMQSIVRNMGYLNKNMNCGITLSDFKNKYPFFTFVLAPDFDLNQSQLPQSGNLRLDIKFENPLTESVSVLVYGVFEKEIQITSSR 1295
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000031049.1 (SMESG000031049.1) HSP 1 Score: 209.92 bits (533), Expect = 7.672e-87 Identity = 97/108 (89.81%), Postives = 103/108 (95.37%), Query Frame = 3 Query: 3 LSKKSDYKETYTQDFDNDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGNILLDIKFSKAVEEPVHVIIYGVFENEIQITANE 326 LSK SDY+ETYTQDF+NDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGN+ LDIKF+KA EPVHV+IYGVFENE+QITAN Sbjct: 287 LSKNSDYRETYTQDFENDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGNLRLDIKFAKATTEPVHVVIYGVFENEVQITANR 394 HSP 2 Score: 130.954 bits (328), Expect = 7.672e-87 Identity = 58/76 (76.32%), Postives = 72/76 (94.74%), Query Frame = 1 Query: 421 VEKIGKQLLGNEFVGVYPLDKIPYLDDNNAIVVNTQSSNLGGEHWIAVYVKPNKIHVFDPMGFYYPALLVSKLQTV 648 V +IGKQ+LG+EF+GV+PLDKIPYL +N AI+VNTQSS+LGGEHW+A+Y+KP+KI VFDPMGFYYPAL+VSKLQT+ Sbjct: 398 VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTM 473
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000031049.1 (SMESG000031049.1) HSP 1 Score: 209.534 bits (532), Expect = 9.753e-87 Identity = 97/108 (89.81%), Postives = 103/108 (95.37%), Query Frame = 3 Query: 3 LSKKSDYKETYTQDFDNDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGNILLDIKFSKAVEEPVHVIIYGVFENEIQITANE 326 LSK SDY+ETYTQDF+NDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGN+ LDIKF+KA EPVHV+IYGVFENE+QITAN Sbjct: 544 LSKNSDYRETYTQDFENDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGNLRLDIKFAKATTEPVHVVIYGVFENEVQITANR 651 HSP 2 Score: 130.954 bits (328), Expect = 9.753e-87 Identity = 58/76 (76.32%), Postives = 72/76 (94.74%), Query Frame = 1 Query: 421 VEKIGKQLLGNEFVGVYPLDKIPYLDDNNAIVVNTQSSNLGGEHWIAVYVKPNKIHVFDPMGFYYPALLVSKLQTV 648 V +IGKQ+LG+EF+GV+PLDKIPYL +N AI+VNTQSS+LGGEHW+A+Y+KP+KI VFDPMGFYYPAL+VSKLQT+ Sbjct: 655 VYRIGKQILGHEFLGVFPLDKIPYLRNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTM 730
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000029226.1 (SMESG000029226.1) HSP 1 Score: 208.764 bits (530), Expect = 1.499e-86 Identity = 97/108 (89.81%), Postives = 102/108 (94.44%), Query Frame = 3 Query: 3 LSKKSDYKETYTQDFDNDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTFVLAPDFDLNQSQLPQNGNILLDIKFSKAVEEPVHVIIYGVFENEIQITANE 326 LSK SDY+ETYTQDF+NDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFT VLAPDFDLNQSQLPQNGN+ LDIKFSKA EPVHV+IYGVFENE+QITAN Sbjct: 947 LSKNSDYRETYTQDFENDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSKYPFFTLVLAPDFDLNQSQLPQNGNLRLDIKFSKATTEPVHVVIYGVFENEVQITANR 1054 HSP 2 Score: 130.954 bits (328), Expect = 1.499e-86 Identity = 58/77 (75.32%), Postives = 73/77 (94.81%), Query Frame = 1 Query: 421 VEKIGKQLLGNEFVGVYPLDKIPYLDDNNAIVVNTQSSNLGGEHWIAVYVKPNKIHVFDPMGFYYPALLVSKLQTVG 651 V +IGKQ+LG++F+GV+PLDKIPYL +N AI+VNTQSS+LGGEHW+A+Y+KP+KI VFDPMGFYYPAL+VSKLQT+G Sbjct: 1058 VYRIGKQILGHKFLGVFPLDKIPYLLNNQAIIVNTQSSHLGGEHWLAIYMKPSKIFVFDPMGFYYPALMVSKLQTMG 1134 The following BLAST results are available for this feature:
BLAST of C2H2-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of C2H2-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010020 ID=SMED30010020|Name=C2H2-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1327bpback to top protein sequence of SMED30010020-orf-1 >SMED30010020-orf-1 ID=SMED30010020-orf-1|Name=SMED30010020-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp LSKKSDYKETYTQDFDNDNYCATYMQSIVRNMGYLDKNLNCGITLEDFKSback to top protein sequence of SMED30010020-orf-2 >SMED30010020-orf-2 ID=SMED30010020-orf-2|Name=SMED30010020-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=140bp MFFKMSSYFIDISVFENEFGGLVIKELCMINTNNILRPLHWVFSANEYHEback to top Annotated Terms
The following terms have been associated with this transcript:
|