AA_TRNA_LIGASE_II domain-containing protein

Overview
NameAA_TRNA_LIGASE_II domain-containing protein
Smed IDSMED30009983
Length (bp)1309
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of AA_TRNA_LIGASE_II domain-containing protein (SMED30009983) t-SNE clustered cells

Violin plots show distribution of expression levels for AA_TRNA_LIGASE_II domain-containing protein (SMED30009983) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009983

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30009983h1SMcG0020663 SmedASXL_007386SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30009983h1SMcG0020663 SmedASXL_007386SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30009983h1SMcG0020663 SmedASXL_074961SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30009983h1SMcG0020663 dd_Smed_v6_21373_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Human
Match: LRR1 (leucine rich repeat protein 1 [Source:HGNC Symbol;Acc:HGNC:19742])

HSP 1 Score: 55.4546 bits (132), Expect = 1.031e-7
Identity = 50/207 (24.15%), Postives = 87/207 (42.03%), Query Frame = 2 
Query:  182 DKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFE--NKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGI-------------------------------------SRMKLSALQIVNCKLERFPVVPLDSN 685
            DK    ++L  NI      F+ EGK T+++K P + I +S+     L  FL  +       N +  ++ ++ + TS + E  +T + I S K +P++K +P +L  L      + + D R+L L+SL  L L    I+++P  I                                     S+ K+ AL +  C+L+    + LD N
Sbjct:   54 DKRGTRYELRENIEQFFTKFVDEGKATVRLKEPPVDICLSKAISSSLKGFLSAMRLAHRGCNVDTPVSTLTPVKTS-EFENFKTKMVITSKKDYPLSKNFPYSLEHLQTSYCGLVRVDMRMLCLKSLRKLDLSHNHIKKLPATIGDLIHLQELNLNDNHLESFSVALCHSTLQKSLRSLDLSKNKIKALPVQFCQLQELKNLKLDDN 259
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Zebrafish
Match: zgc:77287 (zgc:77287 [Source:ZFIN;Acc:ZDB-GENE-040426-2337])

HSP 1 Score: 50.447 bits (119), Expect = 1.838e-6
Identity = 58/159 (36.48%), Postives = 90/159 (56.60%), Query Frame = 2 
Query:  431 LSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMK-LSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISL 904
            L++ S + +P+T+ +P  L++L +   ++ + D R+L L +L  L L    I  +P G+  ++ L+ L + N +LERF      S L  SL LLDLS N L  LP   +  L+ L ++ L+ N+L+ LP   L RL  L+ L  A+N L VLP+ F SL
Sbjct:   50 LTVLSRRDYPLTRSFPPALQQLQVSYCQLSRVDLRVLSLRALRRLDLSNNRIRALPAGLGDLRALAELVLHNNQLERFGDELCCSGLQTSLRLLDLSHNLLPALPAR-FCQLRQLVTLRLDHNRLQRLP-FHLGRLSALRFLSAAHNQLPVLPDGFRSL 206
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Xenopus
Match: rgs2 (regulator of G-protein signaling 2 [Source:Xenbase;Acc:XB-GENE-968776])

HSP 1 Score: 73.559 bits (179), Expect = 1.242e-13
Identity = 81/246 (32.93%), Postives = 125/246 (50.81%), Query Frame = 2 
Query:  182 DKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLE---LLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMK-LSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLA 907
            DKA   +KL NNI  +   FL EGK T+++K P + I +S+     L  F+    L NK  +   + + +++   TS + E  +  + I + K +P+TK +P +L  L +   K+ + D R+L L+ L  L L    I+++P  I  +  L  L + N  LE F VV   + L  +L  LDLS N L  LP  I  + + L S+ L+ N+L  LP   + +L  L+ L    N L  LPN F  L+
Sbjct:   51 DKAGSRYKLENNIEQLFTRFLGEGKATLRLKEPAVDICLSKAEISSLKNFISAVGLANKGADIGTVLLPRLTPAKTS-EIEKPRAKMFITTKKDYPITKSFPYSLEHLQVSYCKLARVDMRMLCLKKLQKLDLSNNHIKKLPKTIGDLVCLQELILNNNLLESFEVVLCSTTLRDTLKSLDLSANKLKALPVQIC-NFKELVSLKLDENELLQLP-FPIGQLSKLRFLSATKNKLQCLPNTFKKLS 293
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Mouse
Match: Lrr1 (leucine rich repeat protein 1 [Source:MGI Symbol;Acc:MGI:1916956])

HSP 1 Score: 72.4034 bits (176), Expect = 2.842e-13
Identity = 65/214 (30.37%), Postives = 110/214 (51.40%), Query Frame = 2 
Query:  182 DKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEI--VTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRM-KLSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALP 814
            D+    +KL  NI  +   F+ EGK T+++K P + I +S+ NP  L   L  +       +++   +S +  V +++ E  +T + I S K +P++K +P  L  L      + + D R+L L++L+ L L    I+++P  I  +  L  L + + +LE F V    S L +SL  LDLS N +  LP   +   + L ++NLN N+L  LP
Sbjct:   62 DRQGTSYKLRENIEQLFTKFVDEGKATVRLKEPPVDICLSKANPGNLKTLLSAMRLAHRGCDVN-TPLSTLKPVKTSEFEKYKTKMVITSKKDYPLSKNFPYFLEHLQASYCSLARVDMRMLCLKNLTKLDLSHNCIKKLPATIGDLTHLQELNLNDNQLETFSVPLCTSTLQKSLHSLDLSKNKIKALPVQ-FCQFRELTNLNLNDNELIHLP 273
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. UniProt/SwissProt
Match: sp|Q96L50|LLR1_HUMAN (Leucine-rich repeat protein 1 OS=Homo sapiens OX=9606 GN=LRR1 PE=1 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 4.953e-7
Identity = 50/207 (24.15%), Postives = 87/207 (42.03%), Query Frame = 2 
Query:  182 DKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFE--NKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGI-------------------------------------SRMKLSALQIVNCKLERFPVVPLDSN 685
            DK    ++L  NI      F+ EGK T+++K P + I +S+     L  FL  +       N +  ++ ++ + TS + E  +T + I S K +P++K +P +L  L      + + D R+L L+SL  L L    I+++P  I                                     S+ K+ AL +  C+L+    + LD N
Sbjct:   54 DKRGTRYELRENIEQFFTKFVDEGKATVRLKEPPVDICLSKAISSSLKGFLSAMRLAHRGCNVDTPVSTLTPVKTS-EFENFKTKMVITSKKDYPLSKNFPYSLEHLQTSYCGLVRVDMRMLCLKSLRKLDLSHNHIKKLPATIGDLIHLQELNLNDNHLESFSVALCHSTLQKSLRSLDLSKNKIKALPVQFCQLQELKNLKLDDN 259
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. TrEMBL
Match: A0A5J4NEX8 (LRR-repeat protein 1 OS=Paragonimus westermani OX=34504 GN=DEA37_0003036 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 6.501e-28
Identity = 100/284 (35.21%), Postives = 163/284 (57.39%), Query Frame = 2 
Query:   98 SGN--KKKFNKCLIVFSLDKKGSRIGFVKNDKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMKLSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLATRLTSCELENAF 943
            SGN    +    L++  ++  G+ +  V +D +S EFKL+NNI  +   + ++G +TI+++VP   I++ + +P  LA+FL++LN +     ++   +S   + +   L    + I+    +P+ K +P +LR+L+  +LK++ FD R+L L SL  L+L    IEQ+P  I+R+ LS L + +  L  +P +  +S LARSL +LDLS N L WLP+D WI L+ L SVNL+   L  LPA  L  +  L  L L +N L+ LP + +SL ++ T     N F
Sbjct:   14 SGNAVHTRPKAALLMLRVNHSGATLKIVFSD-SSKEFKLTNNIVKLYARYFADGNLTIRLRVPAKDIMLRKADPNMLAKFLKMLNDLISGVALAERSISMTGSLSKSLLNPRKMHIKRRDDYPLGKSFPTDLRELNARSLKLRTFDPRLLALSSLCHLELGVNQIEQIPVEIARLNLSVLCLEDNMLTAWPSIEENSPLARSLEILDLSRNKLDWLPDDFWI-LKCLTSVNLSHMGLRGLPAAYLHHVRHLTNLRLDDNHLTCLPFS-VSLLSQCTISVCNNRF 294
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. TrEMBL
Match: A0A564XWP1 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS104 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.743e-21
Identity = 88/270 (32.59%), Postives = 143/270 (52.96%), Query Frame = 2 
Query:  101 GNKKKFNKCLIVFSLDKKGSRIGFVKNDKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMKLSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLAT 910
            G   KF K  +V   ++    + F  + +    F+L++N+  I   F+S+GK ++ +K P+  I   E NP   A     LN +    +   N     + S     + T L ++ LK +PV   +P +L+KL + ++K+  FD+RIL L  LS L +EG  I  +   I+++ L  L + +  +  +P VP +S L+  L+ LDLSGN +  LP D W +L++L ++NLN NQL  +P V L R+  L+ + L NN L  LP +F  L +
Sbjct:   16 GRVNKFRKTRVVIDQNRSAITLSFPSSSQHE-TFRLADNLLLISDRFISQGKFSMTLKKPQRVISFREINPVTAATICRYLNCIKNGTKFKQNSPKTSLNSLSNTSV-TELLVDQLKDYPVGSSFPTSLKKLHIKSIKLDSFDTRILCLTHLSILTIEGSNISVITKDIAKLCLVRLCLNSNSIVSWPSVPRESPLSSFLTYLDLSGNRIIRLPMDFW-NLENLETLNLNDNQLIGVPPVNLHRIRRLRNISLRNNQLRCLPFSFTCLQS 282
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. TrEMBL
Match: A0A5K3F1V4 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 9.925e-21
Identity = 115/400 (28.75%), Postives = 199/400 (49.75%), Query Frame = 2 
Query:   86 SYINSGNKKKFNKCLIVFSLDKKGSRIGFVKNDKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMKLSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLATRLTSCELENAFCDAVRSLTRGDIRHNRPLQPSRLINCAVVA-IQRNPAFYSNLSLYCTLMPRHLWLQANLSRFCHYCYVRVPPDCLRNWNSLQQLCSIRFLFEFSSICP--VLYYVCQSCLTTVA 1276
            SY  S N+  F +  +VF+ +     + ++ + ++S  F+L  N+  + + F  EGK TI++  P   +   E  P+   R   +LNK+   K+      ++     ++E   T L+I + K +P+ + +P +L +L +    + KFDSRI++L+ L  L L   L + +P  I R+ L  L + +  +  +P +  +SNLARSL++LDLS N +SWLPED+W +L  L++ N++ N L  LPA  L +L  L+ ++L+NN L  LP  F  L  R  +  L +   +        +I  +R   P  L + A    +QR   +   L  Y   +P  L L+ ++ R C  C     P+  R   +L+     +F   ++   P  ++Y   + CL  +A
Sbjct:   14 SYYTSYNR--FKETRVVFNKEDFSLTLTYL-SPRSSHCFQLFGNVIKVFRTFAKEGKFTIRLSKPYRDLSFCEVEPRLSLRLCLILNKLVLGKDFDYVVKNKFWV-LEKETTSTKLTITNRKDYPLGRSFPKHLHQLHISGTVLAKFDSRIILLDHLKILYLCDNLFDSVPREIERLSLKTLILRSNAIVHWPSIGKESNLARSLNVLDLSKNCISWLPEDMW-NLSQLQAFNISDNTLFGLPAPNLHKLKILRLINLSNNQLRCLPFGFSRL--RFITASLSD---NPWLPPNTVNINPSRQTAPMTLFHYAAATFLQR---YEKLLPRYEQFLPPRLALELSVLRRCLVCSHVCGPESHRYLQNLRINFVEQFHNGYTDFAPSFIVYLCSELCLFKLA 400
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. TrEMBL
Match: A0A1S3JIP8 (leucine-rich repeat protein 1 OS=Lingula unguis OX=7574 GN=LOC106173628 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.900e-20
Identity = 109/373 (29.22%), Postives = 183/373 (49.06%), Query Frame = 2 
Query:   68 IGNVVLSYINSGNKKKFNKCLIVFSLDKKGSRIGFVKN-----------DKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQ--TSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMK-LSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLATRLTSCELENAFCDAVRSLTRGDIRHNRPLQPSRLINCAVVAIQRNPAFYSNLSLYCTLMPRHLWLQANLSRFCHYCYV 1144
            IGN +L   N   K KF +  +  S+ KK S  G  K            D+   ++ L NNI  +   F+ EGK TI++K P   I + + +P +L  FL ++      +++    +S +  ++ +++ +  T ++I   K +P+T G+P++L  L++    +KK DSRI+ L+ LS L L G  +  +P  +  ++ L+ L + + ++E+FP    DS+L  SL+ L++S N L +LP   + +L  L ++N+N NQL+ LPA     LL L+ L  A NSL  LP  F  L       E  + +C+    L  G       L    L+ CA  +I++    ++   L   L+  H    A L     YC+ 
Sbjct:    9 IGNRLLPSFNMRGKTKFTRTQL--SVGKKPSASGESKEGVVYLMMCTAKDRNGTKYVLKNNIEQLFMKFIDEGKATIRLKDPPHDICLCKADPIQLKSFLNVVKVGALGRDLGNITLSTLAPASAKQVEKPKTRMTILKRKDYPLTTGFPSSLEFLTINGCSLKKVDSRIIQLKHLSHLNLSGNCLTSLPVALGELQHLTELDVSDNQIEQFPQPLGDSSLRNSLTFLNISKNKLKFLPPS-FCALSSLVTLNMNENQLQCLPA-RFGHLLKLRFLSAAKNSLKTLPGTFTQLLL-----EKLDLYCNEF--LLEGPATAVDKLSFPSLLECAARSIKKGRVQHTEEDLPPMLL--HFLKSAKLCLCGGYCFT 368
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. TrEMBL
Match: W6UJS1 (Peptidylprolyl isomerase-like protein OS=Echinococcus granulosus OX=6210 GN=EGR_03784 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 2.049e-20
Identity = 80/229 (34.93%), Postives = 125/229 (54.59%), Query Frame = 2 
Query:  206 LSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMKLSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNA 892
            L  NI ++   F+ EGK TI++  P   +L  +  P    +    L  +   +++ +  +  +     R      L IE  + +P+ K +P +L  L ++N+K+K FDSRIL L +LS L +E   I  +P  +  ++L+ L + + ++E +P V  DS LA SL  L+LS N L+WLPED W +L++L S+ +  N+L ALPA  L  L  L+ L L NN L  LP A
Sbjct:   20 LKGNIVEVYDRFVREGKFTIRLAEPAQDVLFCKVRPDLAQKLCHFLRDMKNGQKVDVRALLGLGGGLSRAPDVGKLVIEQWRDYPLGKPFPPHLESLHIVNIKLKSFDSRILQLANLSMLSVEKTRISSVPKSVECLRLTRLCLKSNEMETWPSVSRDSALASSLLYLNLSENRLAWLPEDFW-NLENLESLLITDNRLGALPAANLHSLKKLRNLQLRNNKLRCLPYA 247
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Cavefish
Match: zgc:77287 (leucine rich repeat protein 1 [Source:NCBI gene;Acc:103039453])

HSP 1 Score: 76.2554 bits (186), Expect = 1.046e-14
Identity = 79/245 (32.24%), Postives = 126/245 (51.43%), Query Frame = 2 
Query:  182 DKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEI--VTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMK-LSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLA 907
            D+A  ++KL  NI      F+ EGK T+++K P + I +S+ +   L  FL          ++    +S +  V + D E  +  L+I S K +P+T  +P +L +L +   K+ + D R+L L++L  L L    I+++P  I  +  L+ L + N  LE F      S L RSL  LDLS N L  LP   +  L+ L ++ L+ N+L  LP   + +L  L+ L  A+N L+VLP  F  L+
Sbjct:   54 DRAGSKYKLKENIEKFFTWFVEEGKATVRLKEPAVDICLSKADANSLKNFLSAARLAHRGSDIDSLPLSTLTPVRARDVEKPKKKLTITSKKDYPLTSNFPYSLEQLQVSYCKLSRVDMRMLSLKALRRLDLSNNHIKKLPATIGDLSCLAELVLHNNHLESFSEAMCLSTLQRSLQHLDLSQNHLQVLPFQ-FCQLRELVNLKLDDNKLLRLP-FRIGQLSKLRFLSAAHNQLTVLPGDFRKLS 296
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Sea Lamprey
Match: zgc:77287 (zgc:77287 [Source:ZFIN;Acc:ZDB-GENE-040426-2337])

HSP 1 Score: 75.8702 bits (185), Expect = 3.247e-15
Identity = 45/164 (27.44%), Postives = 86/164 (52.44%), Query Frame = 2 
Query:  182 DKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVT--SADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMK-LSALQIVNCKLERFP 664
            DKA   +KL  NI  +   F+ EGK T+++K P + + +S+ +   L  FL  L    ++  +S   +S +    S+D E  +  +++ +   +P+  G+P++L+ L + N ++ + DSR+L L  L  L L    + ++   +S +  L+ L + +  LE FP
Sbjct:   75 DKAGSRYKLKGNIEQLFTKFVDEGKATVRLKEPPVDLCLSKASTVGLKGFLSALRLAHQDHNVSSMHISSLAPARSSDVEKPRERMTVLARADYPLIAGFPSSLQTLQVSNCRLSRVDSRMLNLRHLCRLDLSHNHVRELTAALSSLGCLAELVLADNHLEAFP 238
[Close]
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Nematostella
Match: EDO40291 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7S6])

HSP 1 Score: 83.1889 bits (204), Expect = 2.150e-17
Identity = 82/280 (29.29%), Postives = 133/280 (47.50%), Query Frame = 2 
Query:  101 GNKKKFNKCLIVFSL-DKKGSRIGFVKNDKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMKLSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLATRLTSCELEN 937
            GN  K   CL+V +  ++ G++   +          +  NI  I   F+  GK TI+   P   + IS+ +P  L  FL LL    ++ E           ++D E  +T L I+    +P+TK +P NL++LS+ N  MK  D RI  L+ L+ L L G +I+++P GI+ MKLS L +VN  L  F    L  N+      +             ++ + ++L  + L+ N L  LP + + R+  L+    + N+L VLP +   L  RL S ++ N
Sbjct:   36 GNSDKATACLMVCTAKERNGTKYKAI----------IHGNILQIFNKFVQTGKATIRFNDPAHDLFISKADPSSLKSFLSLLKLSAKDIERVALSTLLPAKTSDLEKPKTELIIKQRSDYPLTKPFPGNLQRLSVTNCSMKCVDRRITKLKELTYLSLHGNIIKELPLGIASMKLSELHLVNNGLTEFH-WGLCCNMLSLSLKVLDLRENSIKSLPSVFCNFKNLVHLKLDDNGLHLLP-IHMGRMTSLRFFSASGNNLRVLPLSLSKL--RLDSVDVSN 301
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Medaka
Match: zgc:77287 (leucine rich repeat protein 1 [Source:NCBI gene;Acc:101170558])

HSP 1 Score: 80.4925 bits (197), Expect = 4.188e-16
Identity = 82/245 (33.47%), Postives = 128/245 (52.24%), Query Frame = 2 
Query:  182 DKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEI--VTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRM-KLSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLA 907
            D+A  ++KL NNI      F+ EGK T+++K P + I +S+ +   L  FL       +  + S   +S +  V + D E  +  L+I S K +P+T  +P +L +L +   K+ + D R+L L+ L  L L    I+++P  I  + +LS L + N  LE F      S L ++L +LD+S N L  LP   +  L+ L ++ L+ NQL  LP   + RL  L+ L  ANN LS LP  F  L+
Sbjct:   54 DRAGAKYKLKNNIEKFFTWFVEEGKATVRLKEPAVDICLSKADTNSLKNFLSAARLADKGNDASSLPLSTLAPVRARDVEQPKRKLTIMSKKDYPLTSNFPYSLEQLQVSYCKLAQVDMRMLSLKVLRKLDLSNNHIKKLPATIGDLSRLSELSLHNNHLEAFSEALCLSTLQQTLQVLDISQNRLRCLPAQ-FCQLRELVNLKLDDNQLARLP-FHIGRLSKLRFLSAANNQLSGLPGDFRKLS 296
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Planmine SMEST
Match: SMESG000019852.1 (SMESG000019852.1)

HSP 1 Score: 771.155 bits (1990), Expect = 0.000e+0
Identity = 412/413 (99.76%), Postives = 413/413 (100.00%), Query Frame = 2 
Query:   59 MKIIGNVVLSYINSGNKKKFNKCLIVFSLDKKGSRIGFVKNDKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMKLSALQIVNCKLERFPVVPLDSNLARXXXXXXXXXXXXXXXPEDIWISLQHLRSVNLNGNQLEALPAVXXXXXXXXXXXXXANNSLSVLPNAFISLATRLTSCELENAFCDAVRSLTRGDIRHNRPLQPSRLINCAVVAIQRNPAFYSNLSLYCTLMPRHLWLQANLSRFCHYCYVRVPPDCLRNWNSLQQLCSIRFLFEFSSICPVLYYVCQSCLTTVASTVHQSI 1297
            MKIIGNVVLSYINSGNKKKFNKCLIVFSLDKKGSRIGFVKNDKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLL+EQMPDGISRMKLSALQIVNCKLERFPVVPLDSNLARSLSLLDLSGNGLSWLPEDIWISLQHLRSVNLNGNQLEALPAVGLDRLLGLQKLDLANNSLSVLPNAFISLATRLTSCELENAFCDAVRSLTRGDIRHNRPLQPSRLINCAVVAIQRNPAFYSNLSLYCTLMPRHLWLQANLSRFCHYCYVRVPPDCLRNWNSLQQLCSIRFLFEFSSICPVLYYVCQSCLTTVASTVHQSI
Sbjct:    1 MKIIGNVVLSYINSGNKKKFNKCLIVFSLDKKGSRIGFVKNDKASIEFKLSNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFENKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMNLKMKKFDSRILMLESLSTLQLEGLLVEQMPDGISRMKLSALQIVNCKLERFPVVPLDSNLARSLSLLDLSGNGLSWLPEDIWISLQHLRSVNLNGNQLEALPAVGLDRLLGLQKLDLANNSLSVLPNAFISLATRLTSCELENAFCDAVRSLTRGDIRHNRPLQPSRLINCAVVAIQRNPAFYSNLSLYCTLMPRHLWLQANLSRFCHYCYVRVPPDCLRNWNSLQQLCSIRFLFEFSSICPVLYYVCQSCLTTVASTVHQSI 413
The following BLAST results are available for this feature:
BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
LRR11.031e-724.15leucine rich repeat protein 1 [Source:HGNC Symbol;... [more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:772871.838e-636.48zgc:77287 [Source:ZFIN;Acc:ZDB-GENE-040426-2337][more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rgs21.242e-1332.93regulator of G-protein signaling 2 [Source:Xenbase... [more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Lrr12.842e-1330.37leucine rich repeat protein 1 [Source:MGI Symbol;A... [more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q96L50|LLR1_HUMAN4.953e-724.15Leucine-rich repeat protein 1 OS=Homo sapiens OX=9... [more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4NEX86.501e-2835.21LRR-repeat protein 1 OS=Paragonimus westermani OX=... [more]
A0A564XWP11.743e-2132.59Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A5K3F1V49.925e-2128.75Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A1S3JIP81.900e-2029.22leucine-rich repeat protein 1 OS=Lingula unguis OX... [more]
W6UJS12.049e-2034.93Peptidylprolyl isomerase-like protein OS=Echinococ... [more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:772871.046e-1432.24leucine rich repeat protein 1 [Source:NCBI gene;Ac... [more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:772873.247e-1527.44zgc:77287 [Source:ZFIN;Acc:ZDB-GENE-040426-2337][more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO402912.150e-1729.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:772874.188e-1633.47leucine rich repeat protein 1 [Source:NCBI gene;Ac... [more]
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BLAST of AA_TRNA_LIGASE_II domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000019852.10.000e+099.76SMESG000019852.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009983 ID=SMED30009983|Name=AA_TRNA_LIGASE_II domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1309bp
AATCTAAAATTGATAAGTTAATATTATTTTTTTATTATAAGTTACAAATT
GAAATTTAATGAAAATAATTGGTAATGTCGTCCTTTCCTATATCAATTCT
GGCAATAAAAAGAAATTTAACAAATGCTTGATTGTATTCTCACTTGATAA
GAAAGGTAGCCGTATAGGTTTTGTGAAAAATGATAAGGCAAGCATTGAAT
TTAAGTTAAGTAATAATATTTGTGATATTCGTAAAAACTTCCTTAGTGAA
GGTAAAATTACAATCAAAATAAAAGTTCCAGAAATTTACATTCTAATATC
TGAAACAAATCCAAAAGAATTGGCAAGATTTCTCGAGTTATTGAACAAAG
TATTCGAAAACAAGGAGATGTCGATGAATAAAATGAGTGAAATCGTGACA
AGTGCCGACAGGGAACTGCTGCAAACGAGCTTATCAATTGAATCGCTGAA
GCAATTCCCAGTGACAAAGGGATATCCGGCAAATCTCCGCAAATTGAGCT
TGATGAATTTGAAGATGAAGAAATTCGACAGCCGGATTTTGATGTTAGAA
TCGTTGTCGACTTTGCAGCTAGAGGGATTGCTGATCGAGCAGATGCCAGA
CGGAATCTCGAGGATGAAATTGTCGGCGTTGCAGATTGTTAATTGCAAAT
TGGAACGGTTTCCGGTTGTTCCGCTCGATAGCAACTTGGCAAGGAGCCTT
TCACTCCTGGATTTGAGCGGCAACGGGCTGTCATGGCTACCGGAGGATAT
TTGGATATCTCTGCAGCACTTGCGCTCTGTCAATTTGAATGGAAATCAGT
TGGAAGCGCTGCCTGCTGTTGGATTGGATCGATTGCTGGGCTTGCAGAAA
CTCGACTTAGCTAACAATAGTCTTTCGGTCCTTCCAAATGCATTCATCTC
CTTGGCAACGCGCTTGACCTCGTGTGAATTGGAAAATGCGTTCTGTGACG
CAGTCAGATCGTTGACGCGTGGAGACATCCGGCATAATCGGCCGCTACAG
CCATCGCGCCTTATCAACTGTGCCGTTGTTGCGATCCAGAGAAATCCCGC
ATTTTATAGCAACCTGTCCTTGTATTGCACTCTAATGCCCCGTCACCTAT
GGCTACAGGCCAACCTGTCGAGGTTCTGCCATTATTGCTACGTCCGCGTG
CCCCCAGACTGCTTGCGCAATTGGAATAGCCTTCAACAGCTCTGTAGCAT
CAGATTCTTATTTGAGTTCTCCAGCATTTGTCCTGTGCTTTATTACGTGT
GCCAATCCTGCTTGACAACCGTTGCTAGCACGGTACATCAGTCAATATAG
ATTTTACCC
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protein sequence of SMED30009983-orf-1

>SMED30009983-orf-1 ID=SMED30009983-orf-1|Name=SMED30009983-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=414bp
MKIIGNVVLSYINSGNKKKFNKCLIVFSLDKKGSRIGFVKNDKASIEFKL
SNNICDIRKNFLSEGKITIKIKVPEIYILISETNPKELARFLELLNKVFE
NKEMSMNKMSEIVTSADRELLQTSLSIESLKQFPVTKGYPANLRKLSLMN
LKMKKFDSRILMLESLSTLQLEGLLIEQMPDGISRMKLSALQIVNCKLER
FPVVPLDSNLARSLSLLDLSGNGLSWLPEDIWISLQHLRSVNLNGNQLEA
LPAVGLDRLLGLQKLDLANNSLSVLPNAFISLATRLTSCELENAFCDAVR
SLTRGDIRHNRPLQPSRLINCAVVAIQRNPAFYSNLSLYCTLMPRHLWLQ
ANLSRFCHYCYVRVPPDCLRNWNSLQQLCSIRFLFEFSSICPVLYYVCQS
CLTTVASTVHQSI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0004827proline-tRNA ligase activity
GO:0005524ATP binding
GO:0005515protein binding
Vocabulary: biological process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
GO:0006433prolyl-tRNA aminoacylation
GO:0007165signal transduction
GO:0016567protein ubiquitination
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR003591Leu-rich_rpt_typical-subtyp
IPR032675LRR_dom_sf
IPR001611Leu-rich_rpt
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005886plasma membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003591Leucine-rich repeat, typical subtypeSMARTSM00369LRR_typ_2coord: 235..258
e-value: 0.83
score: 18.7
coord: 259..281
e-value: 2.0
score: 17.4
coord: 163..186
e-value: 220.0
score: 2.2
coord: 212..234
e-value: 26.0
score: 9.8
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 102..322
e-value: 1.0E-20
score: 75.4
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 237..283
e-value: 1.1E-6
score: 28.3
NoneNo IPR availablePANTHERPTHR45752:SF84LEUCINE-RICH REPEAT PROTEIN 1coord: 41..357
NoneNo IPR availablePANTHERPTHR45752FAMILY NOT NAMEDcoord: 41..357
NoneNo IPR availableSUPERFAMILYSSF52058L domain-likecoord: 123..292