Rho-GAP domain-containing protein
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009975 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of Rho-GAP domain-containing protein vs. Ensembl Human
Match: GMIP (GEM interacting protein [Source:HGNC Symbol;Acc:HGNC:24852]) HSP 1 Score: 109.383 bits (272), Expect = 2.194e-23 Identity = 64/195 (32.82%), Postives = 107/195 (54.87%), Query Frame = 2
Query: 1655 VTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK--EAESGE-------VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSK--PRFRMA-DIKEYMDNKSQAKIVQIIIENVETLFG 2203
V +V KC IE L GIYRVS S+++V + ++ + A +L P +++V+K L L+EP++P+HLY+ F+ L K A+ G+ +++ L+ L+ QLP SN + HL ++A EN+M + NLG +F PTL++ PR A + +D+ QA++V+ +I + E +FG
Sbjct: 538 VPFVVTKCTAEIEHRALDVQGIYRVSGSRVRVERLCQAFENGRALVELSGNSPHDVSSVLKRFLQELTEPVIPFHLYDAFISLAKTLHADPGDDPGTPSPSPEVIRSLKTLLVQLPDSNYNTLRHLVAHLFRVAARFMENKMSANNLGIVFGPTLLRPPDGPRAASAIPVTCLLDSGHQAQLVEFLIVHYEQIFG 732 HSP 2 Score: 63.929 bits (154), Expect = 1.962e-9 Identity = 49/195 (25.13%), Postives = 88/195 (45.13%), Query Frame = 2
Query: 92 NPLMSGDQDTENKLSADTITEASLDLSPG-------ITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKL-LDVINRQ-----------RTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
+PL+S D + + +A EAS P E+ L + K VD +L YAK W ++ KEL+ EK+A+ E+E +S K ++ + ++ S + + + ++++ LG L ++ + +Q RTE K RK +KE+W + K+M +A + + + Y QR
Sbjct: 43 DPLLSEDPEPDKTPTATVTNEASCWSGPSPEGPVPLTGEELDLRLIRTKGGVDAALEYAKTWSRYAKELLAWTEKRASYELEFAKSTMKIAEAGKVSIQQQ--------------SHMPLQYIYTLFLEHDLSLGTLAMETVAQQKRDYYQPLAAKRTEIEKWRKEFKEQWMKEQKRMNEAVQALRRAQLQYVQR 223
BLAST of Rho-GAP domain-containing protein vs. Ensembl Human
Match: GMIP (GEM interacting protein [Source:HGNC Symbol;Acc:HGNC:24852]) HSP 1 Score: 109.383 bits (272), Expect = 2.293e-23 Identity = 64/195 (32.82%), Postives = 107/195 (54.87%), Query Frame = 2
Query: 1655 VTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK--EAESGE-------VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSK--PRFRMA-DIKEYMDNKSQAKIVQIIIENVETLFG 2203
V +V KC IE L GIYRVS S+++V + ++ + A +L P +++V+K L L+EP++P+HLY+ F+ L K A+ G+ +++ L+ L+ QLP SN + HL ++A EN+M + NLG +F PTL++ PR A + +D+ QA++V+ +I + E +FG
Sbjct: 564 VPFVVTKCTAEIEHRALDVQGIYRVSGSRVRVERLCQAFENGRALVELSGNSPHDVSSVLKRFLQELTEPVIPFHLYDAFISLAKTLHADPGDDPGTPSPSPEVIRSLKTLLVQLPDSNYNTLRHLVAHLFRVAARFMENKMSANNLGIVFGPTLLRPPDGPRAASAIPVTCLLDSGHQAQLVEFLIVHYEQIFG 758 HSP 2 Score: 63.929 bits (154), Expect = 1.949e-9 Identity = 49/195 (25.13%), Postives = 88/195 (45.13%), Query Frame = 2
Query: 92 NPLMSGDQDTENKLSADTITEASLDLSPG-------ITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKL-LDVINRQ-----------RTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
+PL+S D + + +A EAS P E+ L + K VD +L YAK W ++ KEL+ EK+A+ E+E +S K ++ + ++ S + + + ++++ LG L ++ + +Q RTE K RK +KE+W + K+M +A + + + Y QR
Sbjct: 43 DPLLSEDPEPDKTPTATVTNEASCWSGPSPEGPVPLTGEELDLRLIRTKGGVDAALEYAKTWSRYAKELLAWTEKRASYELEFAKSTMKIAEAGKVSIQQQ--------------SHMPLQYIYTLFLEHDLSLGTLAMETVAQQKRDYYQPLAAKRTEIEKWRKEFKEQWMKEQKRMNEAVQALRRAQLQYVQR 223
BLAST of Rho-GAP domain-containing protein vs. Ensembl Human
Match: ARHGAP10 (Rho GTPase activating protein 10 [Source:HGNC Symbol;Acc:HGNC:26099]) HSP 1 Score: 98.5969 bits (244), Expect = 6.772e-21 Identity = 61/188 (32.45%), Postives = 100/188 (53.19%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPV---------TLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
I+ KCI +E G++ G+YRV + V S ++ L S D NE + T+ + +K L SL EPL+ Y L+ +F+ A+SG V + LV++LP N+++ ++ HL ++N+S++N M NLG +F PTL++ + + MD K Q +V+I+IEN E +F
Sbjct: 76 IIRKCISAVETRGINDQGLYRV----VGVSSKVQRLLSMLMDVKTCNEVDLENSADWEVKTITSALKQYLRSLPEPLMTYELHGDFI---VPAKSGSPESRVNAIHFLVHKLPEKNKEMLDILVKHLTNVSNHSKQNLMTVANLGVVFGPTLMRPQEE----TVAALMDLKFQNIVVEILIENHEKIF 252
BLAST of Rho-GAP domain-containing protein vs. Ensembl Human
Match: ARHGAP29 (Rho GTPase activating protein 29 [Source:HGNC Symbol;Acc:HGNC:30207]) HSP 1 Score: 101.293 bits (251), Expect = 8.088e-21 Identity = 61/209 (29.19%), Postives = 104/209 (49.76%), Query Frame = 2
Query: 1637 QKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESG---------------------EVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+KE D + I+ C IE L GIYRV +K+K + ++L + D+ + +V+K L L EP + + LY EF+DL KE + E++ ++ K + L+ QLP+SN + HLK++ +++EEN+M S NLG +F P+LI+ +P I + +QA++V+ +I + +F
Sbjct: 678 KKEPDGIPFILKICASEIENRALCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEIQHVNEEQETKKNSLEDKKWPNMCIEINRILLKSKDLLRQLPASNFNSLHFLIVHLKRVVDHAEENKMNSKNLGVIFGPSLIRPRPTTAPITISSLAEYSNQARLVEFLITYSQKIF 886 HSP 2 Score: 58.151 bits (139), Expect = 1.323e-7 Identity = 49/151 (32.45%), Postives = 75/151 (49.67%), Query Frame = 2
Query: 188 DDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIID-------QCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
D+ LL K+ ++++ +L+YAK W K+ K +V EKK LE+E R+ K ++ T + M +SL + L+ D Q +Q NK + LL ++ E K RK KE WK + KM++AEN + K K QR
Sbjct: 195 DNVLL--KNTDSIELALSYAKTWSKYTKNIVSWVEKKLNLELESTRNMVKLAEATRTNIGIQEF----MPLQSLFTNALLNDIESSHLLQQTIAALQANKFVQPLLG----RKNEMEKQRKEIKELWKQEQNKMLEAENALKKAKLLCMQR 335
BLAST of Rho-GAP domain-containing protein vs. Ensembl Human
Match: ARHGAP29 (Rho GTPase activating protein 29 [Source:HGNC Symbol;Acc:HGNC:30207]) HSP 1 Score: 100.908 bits (250), Expect = 9.628e-21 Identity = 61/209 (29.19%), Postives = 104/209 (49.76%), Query Frame = 2
Query: 1637 QKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESG---------------------EVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+KE D + I+ C IE L GIYRV +K+K + ++L + D+ + +V+K L L EP + + LY EF+DL KE + E++ ++ K + L+ QLP+SN + HLK++ +++EEN+M S NLG +F P+LI+ +P I + +QA++V+ +I + +F
Sbjct: 678 KKEPDGIPFILKICASEIENRALCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEIQHVNEEQETKKNSLEDKKWPNMCIEINRILLKSKDLLRQLPASNFNSLHFLIVHLKRVVDHAEENKMNSKNLGVIFGPSLIRPRPTTAPITISSLAEYSNQARLVEFLITYSQKIF 886 HSP 2 Score: 59.3066 bits (142), Expect = 5.441e-8 Identity = 49/151 (32.45%), Postives = 75/151 (49.67%), Query Frame = 2
Query: 188 DDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIID-------QCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
D+ LL K+ ++++ +L+YAK W K+ K +V EKK LE+E R+ K ++ T + M +SL + L+ D Q +Q NK + LL ++ E K RK KE WK + KM++AEN + K K QR
Sbjct: 195 DNVLL--KNTDSIELALSYAKTWSKYTKNIVSWVEKKLNLELESTRNMVKLAEATRTNIGIQEF----MPLQSLFTNALLNDIESSHLLQQTIAALQANKFVQPLLG----RKNEMEKQRKEIKELWKQEQNKMLEAENALKKAKLLCMQR 335
BLAST of Rho-GAP domain-containing protein vs. Ensembl Celegans
Match: spv-1 (Spermathecal Physiology Variant [Source:UniProtKB/TrEMBL;Acc:G5ECK6]) HSP 1 Score: 100.523 bits (249), Expect = 1.452e-21 Identity = 62/192 (32.29%), Postives = 99/192 (51.56%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLI--NEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVS------DLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQ 2215
I+ K I+ ++ GL GIYR K K+ + + +D ++ NE P+ LA+VVK L L EPL+ + LY++FV LG E S + S + V +L+ L +LP N + I HL +++ E N M + NL T+ AP+LI P+ R+ M + K ++++I N +FG + Q
Sbjct: 157 IIEKSIDQLQRRGLRAKGIYRTCGVKSKIEEICNAFERSSSDDEVCLDNENPMNLASVVKLYLRKLPEPLLTFELYDDFVRLGTECCSAQASGSNCEEERVEQLRQLARKLPVHNYETLKFIMLHLNRVSWFHEVNLMSTSNLSTVIAPSLIWMSPK-RIDHTSAIMHTQYTNKAIEVMIRNSYHIFGMDRQ 347
BLAST of Rho-GAP domain-containing protein vs. Ensembl Celegans
Match: spv-1 (Spermathecal Physiology Variant [Source:UniProtKB/TrEMBL;Acc:G5ECK6]) HSP 1 Score: 101.679 bits (252), Expect = 1.565e-21 Identity = 63/192 (32.81%), Postives = 99/192 (51.56%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADAD--LINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVS------DLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQ 2215
I+ K I+ ++ GL GIYR K K+ + + +D + L NE P+ LA+VVK L L EPL+ + LY++FV LG E S + S + V +L+ L +LP N + I HL +++ E N M + NL T+ AP+LI P+ R+ M + K ++++I N +FG + Q
Sbjct: 500 IIEKSIDQLQRRGLRAKGIYRTCGVKSKIEEICNAFERSSSDDEVCLDNENPMNLASVVKLYLRKLPEPLLTFELYDDFVRLGTECCSAQASGSNCEEERVEQLRQLARKLPVHNYETLKFIMLHLNRVSWFHEVNLMSTSNLSTVIAPSLIWMSPK-RIDHTSAIMHTQYTNKAIEVMIRNSYHIFGMDRQ 690
BLAST of Rho-GAP domain-containing protein vs. Ensembl Celegans
Match: spv-1 (Spermathecal Physiology Variant [Source:UniProtKB/TrEMBL;Acc:G5ECK6]) HSP 1 Score: 100.523 bits (249), Expect = 1.811e-21 Identity = 62/192 (32.29%), Postives = 99/192 (51.56%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLI--NEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVS------DLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQ 2215
I+ K I+ ++ GL GIYR K K+ + + +D ++ NE P+ LA+VVK L L EPL+ + LY++FV LG E S + S + V +L+ L +LP N + I HL +++ E N M + NL T+ AP+LI P+ R+ M + K ++++I N +FG + Q
Sbjct: 195 IIEKSIDQLQRRGLRAKGIYRTCGVKSKIEEICNAFERSSSDDEVCLDNENPMNLASVVKLYLRKLPEPLLTFELYDDFVRLGTECCSAQASGSNCEEERVEQLRQLARKLPVHNYETLKFIMLHLNRVSWFHEVNLMSTSNLSTVIAPSLIWMSPK-RIDHTSAIMHTQYTNKAIEVMIRNSYHIFGMDRQ 385
BLAST of Rho-GAP domain-containing protein vs. Ensembl Celegans
Match: spv-1 (Spermathecal Physiology Variant [Source:UniProtKB/TrEMBL;Acc:G5ECK6]) HSP 1 Score: 101.293 bits (251), Expect = 2.376e-21 Identity = 63/192 (32.81%), Postives = 99/192 (51.56%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADAD--LINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVS------DLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQ 2215
I+ K I+ ++ GL GIYR K K+ + + +D + L NE P+ LA+VVK L L EPL+ + LY++FV LG E S + S + V +L+ L +LP N + I HL +++ E N M + NL T+ AP+LI P+ R+ M + K ++++I N +FG + Q
Sbjct: 551 IIEKSIDQLQRRGLRAKGIYRTCGVKSKIEEICNAFERSSSDDEVCLDNENPMNLASVVKLYLRKLPEPLLTFELYDDFVRLGTECCSAQASGSNCEEERVEQLRQLARKLPVHNYETLKFIMLHLNRVSWFHEVNLMSTSNLSTVIAPSLIWMSPK-RIDHTSAIMHTQYTNKAIEVMIRNSYHIFGMDRQ 741
BLAST of Rho-GAP domain-containing protein vs. Ensembl Celegans
Match: spv-1 (Spermathecal Physiology Variant [Source:UniProtKB/TrEMBL;Acc:G5ECK6]) HSP 1 Score: 100.908 bits (250), Expect = 3.288e-21 Identity = 63/192 (32.81%), Postives = 99/192 (51.56%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADAD--LINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVS------DLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQ 2215
I+ K I+ ++ GL GIYR K K+ + + +D + L NE P+ LA+VVK L L EPL+ + LY++FV LG E S + S + V +L+ L +LP N + I HL +++ E N M + NL T+ AP+LI P+ R+ M + K ++++I N +FG + Q
Sbjct: 615 IIEKSIDQLQRRGLRAKGIYRTCGVKSKIEEICNAFERSSSDDEVCLDNENPMNLASVVKLYLRKLPEPLLTFELYDDFVRLGTECCSAQASGSNCEEERVEQLRQLARKLPVHNYETLKFIMLHLNRVSWFHEVNLMSTSNLSTVIAPSLIWMSPK-RIDHTSAIMHTQYTNKAIEVMIRNSYHIFGMDRQ 805
BLAST of Rho-GAP domain-containing protein vs. Ensembl Fly
Match: Graf (gene:FBgn0030685 transcript:FBtr0074194) HSP 1 Score: 92.4337 bits (228), Expect = 1.592e-18 Identity = 54/186 (29.03%), Postives = 101/186 (54.30%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVME-SLNSQGADADLINE------QPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+ +CI+V+EI GL GIYR S K+ ++ LN + +D +++ + T+A+ +K L +L+EPL+ Y +++F++ K+ ++ V ++ LV +LP N ++ ++ HL ++ E+N+M NLG +F PTL+ R R + +D K ++ I+I+N E +F
Sbjct: 402 FIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQET---LNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLL----RPREESVAAILDIKFNNIVINILIDNYERIF 580
BLAST of Rho-GAP domain-containing protein vs. Ensembl Fly
Match: Graf (gene:FBgn0030685 transcript:FBtr0345133) HSP 1 Score: 92.4337 bits (228), Expect = 1.592e-18 Identity = 54/186 (29.03%), Postives = 101/186 (54.30%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVME-SLNSQGADADLINE------QPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+ +CI+V+EI GL GIYR S K+ ++ LN + +D +++ + T+A+ +K L +L+EPL+ Y +++F++ K+ ++ V ++ LV +LP N ++ ++ HL ++ E+N+M NLG +F PTL+ R R + +D K ++ I+I+N E +F
Sbjct: 402 FIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQET---LNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLL----RPREESVAAILDIKFNNIVINILIDNYERIF 580
BLAST of Rho-GAP domain-containing protein vs. Ensembl Fly
Match: Graf (gene:FBgn0030685 transcript:FBtr0074197) HSP 1 Score: 92.4337 bits (228), Expect = 1.592e-18 Identity = 54/186 (29.03%), Postives = 101/186 (54.30%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVME-SLNSQGADADLINE------QPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+ +CI+V+EI GL GIYR S K+ ++ LN + +D +++ + T+A+ +K L +L+EPL+ Y +++F++ K+ ++ V ++ LV +LP N ++ ++ HL ++ E+N+M NLG +F PTL+ R R + +D K ++ I+I+N E +F
Sbjct: 402 FIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQET---LNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLL----RPREESVAAILDIKFNNIVINILIDNYERIF 580
BLAST of Rho-GAP domain-containing protein vs. Ensembl Fly
Match: Graf (gene:FBgn0030685 transcript:FBtr0074198) HSP 1 Score: 92.4337 bits (228), Expect = 1.592e-18 Identity = 54/186 (29.03%), Postives = 101/186 (54.30%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVME-SLNSQGADADLINE------QPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+ +CI+V+EI GL GIYR S K+ ++ LN + +D +++ + T+A+ +K L +L+EPL+ Y +++F++ K+ ++ V ++ LV +LP N ++ ++ HL ++ E+N+M NLG +F PTL+ R R + +D K ++ I+I+N E +F
Sbjct: 402 FIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQET---LNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLL----RPREESVAAILDIKFNNIVINILIDNYERIF 580
BLAST of Rho-GAP domain-containing protein vs. Ensembl Fly
Match: Graf (gene:FBgn0030685 transcript:FBtr0340306) HSP 1 Score: 92.4337 bits (228), Expect = 1.699e-18 Identity = 54/186 (29.03%), Postives = 101/186 (54.30%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVME-SLNSQGADADLINE------QPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+ +CI+V+EI GL GIYR S K+ ++ LN + +D +++ + T+A+ +K L +L+EPL+ Y +++F++ K+ ++ V ++ LV +LP N ++ ++ HL ++ E+N+M NLG +F PTL+ R R + +D K ++ I+I+N E +F
Sbjct: 411 FIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQET---LNQRVNEVHKLVYKLPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLL----RPREESVAAILDIKFNNIVINILIDNYERIF 589
BLAST of Rho-GAP domain-containing protein vs. Ensembl Zebrafish
Match: gmip (GEM interacting protein [Source:ZFIN;Acc:ZDB-GENE-130530-823]) HSP 1 Score: 111.694 bits (278), Expect = 2.114e-24 Identity = 65/196 (33.16%), Postives = 106/196 (54.08%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKE----------AES-GEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFG 2203
D+V +VL+C E IE L G+YR+S SK +V + ++ Q DL + P + +V+K L EPL+ + LYN+F+ +GKE AES G V ++V +L+ L+ +LP N + HL ++A E+N+M NLG +F PTL++ ++ I ++ QA +V+ +I N + +FG
Sbjct: 562 DSVPFVVLRCTEEIENRALGVQGVYRISGSKPRVLKLCQAFEIQKDQVDLSDLSPHDITSVLKHFFKELPEPLLTFDLYNDFIAVGKEIQRLSEKDHAAESPGIVENIVRRLRELIGRLPVCNNITVQHMMAHLNRVAEQFEDNKMSPGNLGIVFGPTLLRPLVSGDVSMIA-LLETSYQALLVEFMISNYDQVFG 756 HSP 2 Score: 65.855 bits (159), Expect = 3.022e-10 Identity = 43/172 (25.00%), Postives = 84/172 (48.84%), Query Frame = 2
Query: 179 ITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLG--------KLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQAL 670
I +D L ++ V+ +L YAK+WC++ K+L+ +K+ +LE E +S + + ++ V+ M P+++ I Q E +++ + + ++ +R E K R+ +KE+W + K+M DA + + + + Y QRC EEL A+A+
Sbjct: 100 IAQDADLSLCHCEDGVELALQYAKMWCRYAKDLLTWMDKRISLEQEFAKSIMRAADAAKSCVAQQDMM--PLQN--------IYTQALEHDLKTSASAKQSAENIQQRCYQALSAKRNEIDKWRREFKEQWSKEQKRMHDAVSALKRARQQYMQRC-------EELEKAKAM 254
BLAST of Rho-GAP domain-containing protein vs. Ensembl Zebrafish
Match: gmip (GEM interacting protein [Source:ZFIN;Acc:ZDB-GENE-130530-823]) HSP 1 Score: 110.923 bits (276), Expect = 3.974e-24 Identity = 65/196 (33.16%), Postives = 106/196 (54.08%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKE----------AES-GEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFG 2203
D+V +VL+C E IE L G+YR+S SK +V + ++ Q DL + P + +V+K L EPL+ + LYN+F+ +GKE AES G V ++V +L+ L+ +LP N + HL ++A E+N+M NLG +F PTL++ ++ I ++ QA +V+ +I N + +FG
Sbjct: 439 DSVPFVVLRCTEEIENRALGVQGVYRISGSKPRVLKLCQAFEIQKDQVDLSDLSPHDITSVLKHFFKELPEPLLTFDLYNDFIAVGKEIQRLSEKDHAAESPGIVENIVRRLRELIGRLPVCNNITVQHMMAHLNRVAEQFEDNKMSPGNLGIVFGPTLLRPLVSGDVSMIA-LLETSYQALLVEFMISNYDQVFG 633 HSP 2 Score: 53.9138 bits (128), Expect = 1.438e-6 Identity = 35/148 (23.65%), Postives = 71/148 (47.97%), Query Frame = 2
Query: 251 LWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLG--------KLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQAL 670
+WC++ K+L+ +K+ +LE E +S + + ++ V+ M P+++ I Q E +++ + + ++ +R E K R+ +KE+W + K+M DA + + + + Y QRC EEL A+A+
Sbjct: 1 MWCRYAKDLLTWMDKRISLEQEFAKSIMRAADAAKSCVAQQDMM--PLQN--------IYTQALEHDLKTSASAKQSAENIQQRCYQALSAKRNEIDKWRREFKEQWSKEQKRMHDAVSALKRARQQYMQRC-------EELEKAKAM 131
BLAST of Rho-GAP domain-containing protein vs. Ensembl Zebrafish
Match: arhgap45a (Rho GTPase activating protein 45a [Source:ZFIN;Acc:ZDB-GENE-140106-47]) HSP 1 Score: 110.153 bits (274), Expect = 9.275e-24 Identity = 64/207 (30.92%), Postives = 105/207 (50.72%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEA-----------------------ESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
DAV I+ KCI IE L GIYRV+ K +V + ++ + +L P ++NV+K L L EP++P+ +YNE + L KE+ EV LV KL+ ++ +LP++N I HL++++ +EN+M + NLG +F PTL++ KP + +D QA+IV+ +I +++F
Sbjct: 785 DAVPFIIKKCITEIERRALKMKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPIMPFRMYNELMGLAKESLNSDASESTDGVKSPELVDRGPETEAEVLTLVDKLREVLKRLPNANIATLKYIIQHLRRVSELEQENKMSASNLGIVFGPTLMRPKPTGATVSLSSLVDYPHQARIVEALIVFQQSIF 991 HSP 2 Score: 63.929 bits (154), Expect = 1.272e-9 Identity = 40/154 (25.97%), Postives = 77/154 (50.00%), Query Frame = 2
Query: 197 LLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQ-----CKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLR 643
+L ++ + VD +L+Y+K K+ K+++ +K+ LE E + + +QS + + M P+ LS+ L ++Q C + NN L + ++R E K R+ KE+W+ +K+ DAE+ + K + Y RC++ + R
Sbjct: 288 ILLQRSEGGVDSALSYSKSIAKYMKDIISYVDKRIALENEFSKGLQRLYQSCKQNIIQDHM---PL----LSIFSLALEQDSEQSCGLQQTTNNIYTHSFLQPMTQRRQEHEKKRRDIKEQWQRAKRKLADAESNLRKARQLYMSRCEEYERAR 434
BLAST of Rho-GAP domain-containing protein vs. Ensembl Zebrafish
Match: arhgap29a (Rho GTPase activating protein 29a [Source:ZFIN;Acc:ZDB-GENE-030131-9510]) HSP 1 Score: 105.531 bits (262), Expect = 2.268e-22 Identity = 61/205 (29.76%), Postives = 107/205 (52.20%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAES---------------------GEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + I+ KC IE L+ GIYRV+ +K +V + ++ + +L + P ++NV+K L L EPL+ + YN+F+ L KE++S E++ ++ K++ L+ QLP ++ K + HL +++ ++EN+M S NLG +F PTLIK + + +D QA IV+++I + E +F
Sbjct: 550 DGIPFIIKKCTSEIENRALNIKGIYRVNGAKSRVEKLCQAFENGKDLVELSDLYPHDISNVLKLYLRQLPEPLILFRYYNDFIGLAKESQSIIVDEVEASRGCPTSDTPQISVELNRVLFKIKDLLRQLPPAHYKTLQFLIQHLHRVSERADENKMTSSNLGIIFGPTLIKPRQADAEVSLSSLVDYPYQALIVELLIRHYEMIF 754 HSP 2 Score: 60.4622 bits (145), Expect = 1.510e-8 Identity = 45/153 (29.41%), Postives = 79/153 (51.63%), Query Frame = 2
Query: 191 DKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKL--GKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLR 643
D L R D + V+ +L YAK W K+ KEL+ +K+ ++IE +S++K +S + S P R +S + I+ + I +Q L K + + +++E K RK KE+W+ + KKM +A++ + K + QR ++ K R
Sbjct: 39 DGALLRSD-SGVESALLYAKAWSKYTKELLAWVDKRLNMDIECAKSYAKMAESAKALASQQEHM--PFRDIYISAFRNDIEYSQLI-MQTTAALQANKFMQPLQARKSELDKLRKEVKEQWQREQKKMHEADSALRKARLLQAQRQEEYEKAR 187
BLAST of Rho-GAP domain-containing protein vs. Ensembl Zebrafish
Match: arhgap42b (Rho GTPase activating protein 42b [Source:ZFIN;Acc:ZDB-GENE-120217-5]) HSP 1 Score: 104.76 bits (260), Expect = 3.565e-22 Identity = 64/184 (34.78%), Postives = 107/184 (58.15%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANV-----VKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
V KCI+++E+ GL+T G+YRV KV +M ++ S A +DL + P T N +K+ L LSEPL+ Y L+++F+ +S + + V + LV++LP NR++ ++ HL ++++SE N M NLG +F PTL++S+ + M+ K Q +V+I+IEN+E +F
Sbjct: 399 FVRKCIQLVEVRGLNTMGLYRVGGINSKVQKLMTTVFSPKAASDL-DLDPDTWDNKTITSGLKNYLRCLSEPLMTYKLHSDFL---MAVKSDDQNYRVCAVHALVHKLPDKNREMLEILIKHLLNVSSHSESNMMTVSNLGVIFGPTLMRSQEE----TVAAMMNIKFQNIVVEILIENLEKIF 574
BLAST of Rho-GAP domain-containing protein vs. Ensembl Xenopus
Match: arhgap45 (Rho GTPase activating protein 45 [Source:NCBI gene;Acc:100125789]) HSP 1 Score: 103.605 bits (257), Expect = 1.247e-21 Identity = 60/202 (29.70%), Postives = 98/202 (48.51%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESG------------------EVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + ++ KC+ IE L+ GIYRV+ K +V + ++ + +L P L+NV+K L L EPL+P+ LYN + L KE+ G EV +V +L+ L+ LP NR + HL +++ + N+M NLG +F P L++ +P + +D QA+IV+ +I T+F
Sbjct: 613 DHIPFLIRKCVSEIEERALTMKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDLSNVLKLYLRQLPEPLIPFRLYNGLMGLAKESLRGTETGKGSRLQDKGPNTEPEVLSMVVQLKELLQDLPVDNRTTLQYLVKHLCRVSEQEQLNKMSPSNLGIVFGPALMRPRPTDATVSLSSLVDYPHQARIVETLIIFYSTIF 814 HSP 2 Score: 60.4622 bits (145), Expect = 1.955e-8 Identity = 41/157 (26.11%), Postives = 76/157 (48.41%), Query Frame = 2
Query: 161 LDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRS-ESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQ 628
L+ + ED ++ ++ V +L YAK K+ K+L EK+ LE+E + K +++ ++ P +S S++L + + + + L IN +R E K RK KE+W+ +K+M+AE+ + K + AY QR ++
Sbjct: 126 LEAGSALAEDVDVILQRSDGGVQAALLYAKNMAKYLKDLSNYIEKRTILEMEYAKGLQKLVNAYKGALNQETHM--PFQSIYSVALEQDLEHGNGVLHTALTLQHQTFLQPINMRRQEHEKRRKEVKEQWQRAQRKLMEAESNLRKARQAYMQRSEE 280
BLAST of Rho-GAP domain-containing protein vs. Ensembl Xenopus
Match: arhgap45 (Rho GTPase activating protein 45 [Source:NCBI gene;Acc:100125789]) HSP 1 Score: 103.605 bits (257), Expect = 1.350e-21 Identity = 60/202 (29.70%), Postives = 98/202 (48.51%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESG------------------EVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + ++ KC+ IE L+ GIYRV+ K +V + ++ + +L P L+NV+K L L EPL+P+ LYN + L KE+ G EV +V +L+ L+ LP NR + HL +++ + N+M NLG +F P L++ +P + +D QA+IV+ +I T+F
Sbjct: 637 DHIPFLIRKCVSEIEERALTMKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDLSNVLKLYLRQLPEPLIPFRLYNGLMGLAKESLRGTETGKGSRLQDKGPNTEPEVLSMVVQLKELLQDLPVDNRTTLQYLVKHLCRVSEQEQLNKMSPSNLGIVFGPALMRPRPTDATVSLSSLVDYPHQARIVETLIIFYSTIF 838 HSP 2 Score: 60.077 bits (144), Expect = 2.606e-8 Identity = 41/157 (26.11%), Postives = 76/157 (48.41%), Query Frame = 2
Query: 161 LDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRS-ESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQ 628
L+ + ED ++ ++ V +L YAK K+ K+L EK+ LE+E + K +++ ++ P +S S++L + + + + L IN +R E K RK KE+W+ +K+M+AE+ + K + AY QR ++
Sbjct: 150 LEAGSALAEDVDVILQRSDGGVQAALLYAKNMAKYLKDLSNYIEKRTILEMEYAKGLQKLVNAYKGALNQETHM--PFQSIYSVALEQDLEHGNGVLHTALTLQHQTFLQPINMRRQEHEKRRKEVKEQWQRAQRKLMEAESNLRKARQAYMQRSEE 304
BLAST of Rho-GAP domain-containing protein vs. Ensembl Xenopus
Match: arhgap45 (Rho GTPase activating protein 45 [Source:NCBI gene;Acc:100125789]) HSP 1 Score: 103.219 bits (256), Expect = 1.374e-21 Identity = 60/202 (29.70%), Postives = 98/202 (48.51%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESG------------------EVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + ++ KC+ IE L+ GIYRV+ K +V + ++ + +L P L+NV+K L L EPL+P+ LYN + L KE+ G EV +V +L+ L+ LP NR + HL +++ + N+M NLG +F P L++ +P + +D QA+IV+ +I T+F
Sbjct: 585 DHIPFLIRKCVSEIEERALTMKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDLSNVLKLYLRQLPEPLIPFRLYNGLMGLAKESLRGTETGKGSRLQDKGPNTEPEVLSMVVQLKELLQDLPVDNRTTLQYLVKHLCRVSEQEQLNKMSPSNLGIVFGPALMRPRPTDATVSLSSLVDYPHQARIVETLIIFYSTIF 786 HSP 2 Score: 60.077 bits (144), Expect = 2.544e-8 Identity = 41/157 (26.11%), Postives = 76/157 (48.41%), Query Frame = 2
Query: 161 LDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRS-ESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQ 628
L+ + ED ++ ++ V +L YAK K+ K+L EK+ LE+E + K +++ ++ P +S S++L + + + + L IN +R E K RK KE+W+ +K+M+AE+ + K + AY QR ++
Sbjct: 125 LEAGSALAEDVDVILQRSDGGVQAALLYAKNMAKYLKDLSNYIEKRTILEMEYAKGLQKLVNAYKGALNQETHM--PFQSIYSVALEQDLEHGNGVLHTALTLQHQTFLQPINMRRQEHEKRRKEVKEQWQRAQRKLMEAESNLRKARQAYMQRSEE 279
BLAST of Rho-GAP domain-containing protein vs. Ensembl Xenopus
Match: arhgap45 (Rho GTPase activating protein 45 [Source:NCBI gene;Acc:100125789]) HSP 1 Score: 103.605 bits (257), Expect = 1.421e-21 Identity = 60/202 (29.70%), Postives = 98/202 (48.51%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESG------------------EVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + ++ KC+ IE L+ GIYRV+ K +V + ++ + +L P L+NV+K L L EPL+P+ LYN + L KE+ G EV +V +L+ L+ LP NR + HL +++ + N+M NLG +F P L++ +P + +D QA+IV+ +I T+F
Sbjct: 664 DHIPFLIRKCVSEIEERALTMKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDLSNVLKLYLRQLPEPLIPFRLYNGLMGLAKESLRGTETGKGSRLQDKGPNTEPEVLSMVVQLKELLQDLPVDNRTTLQYLVKHLCRVSEQEQLNKMSPSNLGIVFGPALMRPRPTDATVSLSSLVDYPHQARIVETLIIFYSTIF 865 HSP 2 Score: 58.5362 bits (140), Expect = 8.159e-8 Identity = 43/163 (26.38%), Postives = 72/163 (44.17%), Query Frame = 2
Query: 161 LDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSK------QFQS-FETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQ 628
L+ + ED ++ ++ V +L YAK K+ K+L EK+ LE+E + K FQS + + + G + +L+L L IN +R E K RK KE+W+ +K+M+AE+ + K + AY QR ++
Sbjct: 244 LEAGSALAEDVDVILQRSDGGVQAALLYAKNMAKYLKDLSNYIEKRTILEMEYAKGLQKLVNTHMPFQSIYSVALEQDLEHGNGVLHTALTLQHQT-----------------FLQPINMRRQEHEKRRKEVKEQWQRAQRKLMEAESNLRKARQAYMQRSEE 389
BLAST of Rho-GAP domain-containing protein vs. Ensembl Xenopus
Match: arhgap45 (Rho GTPase activating protein 45 [Source:NCBI gene;Acc:100125789]) HSP 1 Score: 103.605 bits (257), Expect = 1.450e-21 Identity = 60/202 (29.70%), Postives = 98/202 (48.51%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESG------------------EVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + ++ KC+ IE L+ GIYRV+ K +V + ++ + +L P L+NV+K L L EPL+P+ LYN + L KE+ G EV +V +L+ L+ LP NR + HL +++ + N+M NLG +F P L++ +P + +D QA+IV+ +I T+F
Sbjct: 712 DHIPFLIRKCVSEIEERALTMKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDLSNVLKLYLRQLPEPLIPFRLYNGLMGLAKESLRGTETGKGSRLQDKGPNTEPEVLSMVVQLKELLQDLPVDNRTTLQYLVKHLCRVSEQEQLNKMSPSNLGIVFGPALMRPRPTDATVSLSSLVDYPHQARIVETLIIFYSTIF 913 HSP 2 Score: 60.8474 bits (146), Expect = 1.460e-8 Identity = 41/157 (26.11%), Postives = 76/157 (48.41%), Query Frame = 2
Query: 161 LDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRS-ESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQ 628
L+ + ED ++ ++ V +L YAK K+ K+L EK+ LE+E + K +++ ++ P +S S++L + + + + L IN +R E K RK KE+W+ +K+M+AE+ + K + AY QR ++
Sbjct: 253 LEAGSALAEDVDVILQRSDGGVQAALLYAKNMAKYLKDLSNYIEKRTILEMEYAKGLQKLVNAYKGALNQETHM--PFQSIYSVALEQDLEHGNGVLHTALTLQHQTFLQPINMRRQEHEKRRKEVKEQWQRAQRKLMEAESNLRKARQAYMQRSEE 407
BLAST of Rho-GAP domain-containing protein vs. Ensembl Mouse
Match: Gmip (Gem-interacting protein [Source:MGI Symbol;Acc:MGI:1926066]) HSP 1 Score: 110.538 bits (275), Expect = 7.437e-24 Identity = 63/195 (32.31%), Postives = 107/195 (54.87%), Query Frame = 2
Query: 1655 VTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK--EAESGE-------VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSK--PRFRMAD-IKEYMDNKSQAKIVQIIIENVETLFG 2203
V ++ +C IE L GIYRVS S+++V + ++ + A +L P + +V+K L L++P+VP+HLY+ F+ L K A+ G+ +++ L+ L+ QLP SN + HL ++A EEN+M + NLG +F PTL++ PR A + +D+ QA++V+ +I + E +FG
Sbjct: 563 VPFVITRCTAEIEHRALGLQGIYRVSGSRVRVERLCQAFENGRALVELSGNSPHDITSVLKRFLQELTDPVVPFHLYDAFISLAKTLHADPGDDPGTPNPSPEIIRSLKTLLVQLPDSNYSTLRHLVAHLFRVAARFEENKMSANNLGIVFGPTLLRPPDGPRATGASPVACLLDSGHQAQLVEFLIVHYEQIFG 757 HSP 2 Score: 58.5362 bits (140), Expect = 6.346e-8 Identity = 50/199 (25.13%), Postives = 94/199 (47.24%), Query Frame = 2
Query: 92 NPLMSGDQDTENKLSADTITEAS------LDLSP----GITEDD-KLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKL-LDVINRQ-----------RTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
+P+ D + + +A +TE + DLSP +TE++ L + K VD +L YAK W ++ KEL+ +K+A E+E +S K ++ + ++ L S++ + + ++++ LG L L+ + +Q R E K RK +KE+W + K+M +A + +++ Y QR
Sbjct: 43 DPVRREDLEPDKADTATVVTEENSEASSWRDLSPEGPAPLTEEELDLRLIRTKGGVDAALEYAKAWSRYAKELLAWTDKRANYELEFAKSIMKLAEAGKVSI--------------LQQSQMPLQYIYTLFLEHDLSLGALALETVAQQKRDYYQPLAAKRMEIEKWRKEFKEQWLKEQKRMNEAVQALRRSELQYIQR 227
BLAST of Rho-GAP domain-containing protein vs. Ensembl Mouse
Match: Gmip (Gem-interacting protein [Source:MGI Symbol;Acc:MGI:1926066]) HSP 1 Score: 110.153 bits (274), Expect = 8.062e-24 Identity = 63/195 (32.31%), Postives = 107/195 (54.87%), Query Frame = 2
Query: 1655 VTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK--EAESGE-------VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSK--PRFRMAD-IKEYMDNKSQAKIVQIIIENVETLFG 2203
V ++ +C IE L GIYRVS S+++V + ++ + A +L P + +V+K L L++P+VP+HLY+ F+ L K A+ G+ +++ L+ L+ QLP SN + HL ++A EEN+M + NLG +F PTL++ PR A + +D+ QA++V+ +I + E +FG
Sbjct: 563 VPFVITRCTAEIEHRALGLQGIYRVSGSRVRVERLCQAFENGRALVELSGNSPHDITSVLKRFLQELTDPVVPFHLYDAFISLAKTLHADPGDDPGTPNPSPEIIRSLKTLLVQLPDSNYSTLRHLVAHLFRVAARFEENKMSANNLGIVFGPTLLRPPDGPRATGASPVACLLDSGHQAQLVEFLIVHYEQIFG 757 HSP 2 Score: 58.151 bits (139), Expect = 7.979e-8 Identity = 50/199 (25.13%), Postives = 94/199 (47.24%), Query Frame = 2
Query: 92 NPLMSGDQDTENKLSADTITE------ASLDLSP----GITEDD-KLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKL-LDVINRQ-----------RTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
+P+ D + + +A +TE + DLSP +TE++ L + K VD +L YAK W ++ KEL+ +K+A E+E +S K ++ + ++ L S++ + + ++++ LG L L+ + +Q R E K RK +KE+W + K+M +A + +++ Y QR
Sbjct: 43 DPVRREDLEPDKADTATVVTEENSEASSWRDLSPEGPAPLTEEELDLRLIRTKGGVDAALEYAKAWSRYAKELLAWTDKRANYELEFAKSIMKLAEAGKVSI--------------LQQSQMPLQYIYTLFLEHDLSLGALALETVAQQKRDYYQPLAAKRMEIEKWRKEFKEQWLKEQKRMNEAVQALRRSELQYIQR 227
BLAST of Rho-GAP domain-containing protein vs. Ensembl Mouse
Match: Arhgap29 (Rho GTPase activating protein 29 [Source:MGI Symbol;Acc:MGI:2443818]) HSP 1 Score: 102.449 bits (254), Expect = 1.706e-21 Identity = 61/209 (29.19%), Postives = 105/209 (50.24%), Query Frame = 2
Query: 1637 QKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAE---------------------SGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+KE D + ++ C IE L GIYRV +K+K + ++L + D+ + +V+K L L EP + + LY EF+DL KE + S EV+ ++ K + L+ QLP+S+ + HL+++ +++EEN+M S NLG +F PTLI+ +P I + +QA++V+ +I + +F
Sbjct: 273 KKEPDGIPFVLKICASEIENRALCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEIQHVNEEQEAKKDSPEDKKHPHVSIEVNRILLKSKDLLRQLPASHFNSLHYLIAHLRRVVDHAEENKMNSKNLGVIFGPTLIRPRPTTAPVTISSLAEYSNQARLVEFLITYSQKIF 481
BLAST of Rho-GAP domain-containing protein vs. Ensembl Mouse
Match: Arhgap29 (Rho GTPase activating protein 29 [Source:MGI Symbol;Acc:MGI:2443818]) HSP 1 Score: 101.679 bits (252), Expect = 3.982e-21 Identity = 61/209 (29.19%), Postives = 105/209 (50.24%), Query Frame = 2
Query: 1637 QKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAE---------------------SGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+KE D + ++ C IE L GIYRV +K+K + ++L + D+ + +V+K L L EP + + LY EF+DL KE + S EV+ ++ K + L+ QLP+S+ + HL+++ +++EEN+M S NLG +F PTLI+ +P I + +QA++V+ +I + +F
Sbjct: 680 KKEPDGIPFVLKICASEIENRALCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEIQHVNEEQEAKKDSPEDKKHPHVSIEVNRILLKSKDLLRQLPASHFNSLHYLIAHLRRVVDHAEENKMNSKNLGVIFGPTLIRPRPTTAPVTISSLAEYSNQARLVEFLITYSQKIF 888 HSP 2 Score: 53.1434 bits (126), Expect = 3.220e-6 Identity = 47/152 (30.92%), Postives = 71/152 (46.71%), Query Frame = 2
Query: 185 EDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIID-------QCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
E D LL K+ ++++ +L+YAK W K+ K +V EKK LE+E R+ K SS+ M +SL + L+ D Q +Q NK + LL ++ E K RK K+ WK K+++ E + K K QR
Sbjct: 193 ELDNLLL-KNTDSIELALSYAKTWSKYTKNIVSWVEKKLNLELESTRNIVK----LAEATRSSIGIQEFMPLQSLFTNALLSDIHSSHLLQQTIAALQANKFVQPLLG----RKNEMEKQRKEIKDLWKQQQNKLLETETALKKAKLLCMQR 335
BLAST of Rho-GAP domain-containing protein vs. Ensembl Mouse
Match: Arhgap45 (Rho GTPase activating protein 45 [Source:MGI Symbol;Acc:MGI:1917969]) HSP 1 Score: 100.908 bits (250), Expect = 7.110e-21 Identity = 59/204 (28.92%), Postives = 101/204 (49.51%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDL--------------------GKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D V IV KC+ IE L T GIYRV+ K +V + ++ + +L P ++NV+K L L EPL+ + Y+E V L G E+E+ ++ +V +L+ L+ LP+ NR + HL++I ++N+M NLG +F PTL++ +P + +D QA++++ +I + +F
Sbjct: 769 DGVPFIVKKCVCEIERRALHTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRFYHELVGLAKDSLKAEAEAKAASRGRQGGSESEAATLA-MVGRLRELMQDLPAENRATLLYLLKHLRRIVEMEQDNKMTPGNLGIVFGPTLLRPRPTDATVSLSSLVDYPHQARVIETLIVHYGLVF 971 HSP 2 Score: 58.9214 bits (141), Expect = 4.790e-8 Identity = 78/354 (22.03%), Postives = 136/354 (38.42%), Query Frame = 2
Query: 185 EDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKL-----LDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEK-QLSNKPDIYSEKFQVELY 1228
E+ +L ++ + VD +L YAK ++ K+L+ EK+ TLE+E + K + +V+ P+ LS+ L ++Q E + G L + + +R E + RK KE W +K+ +AE + K K Y QRC+ K R ++ A + Q TG TA SK +DK + + K E M +Y+ V +A + E E K+ L I+ +SD+ +K Y +++ + + +L + Y P Y + + Q +PD+ ++ E Y
Sbjct: 268 EEVDMLLQRCEGGVDAALQYAKDMARYMKDLISYLEKRTTLEMEFAKGLQKVVHNCRQSVTHEPHM--PL----LSIYSLALEQDLEFGHGMVQAAGTLQTQTFMQPLTLRRLEHERRRKEIKESWHRAQRKLQEAEANLRKAKQGYKQRCEDHDKARLQVAKA--------EEEQQGTGPGAGTA----------------------------------------ASK---ALDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQLMHMQTAPLPVNFQMLCESSKLYDPGQQYASHVRQLQRGEEPDV---RYDFEPY 561
BLAST of Rho-GAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6PGG2|GMIP_MOUSE (GEM-interacting protein OS=Mus musculus OX=10090 GN=Gmip PE=1 SV=1) HSP 1 Score: 110.538 bits (275), Expect = 5.202e-23 Identity = 63/195 (32.31%), Postives = 107/195 (54.87%), Query Frame = 2
Query: 1655 VTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK--EAESGE-------VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSK--PRFRMAD-IKEYMDNKSQAKIVQIIIENVETLFG 2203
V ++ +C IE L GIYRVS S+++V + ++ + A +L P + +V+K L L++P+VP+HLY+ F+ L K A+ G+ +++ L+ L+ QLP SN + HL ++A EEN+M + NLG +F PTL++ PR A + +D+ QA++V+ +I + E +FG
Sbjct: 563 VPFVITRCTAEIEHRALGLQGIYRVSGSRVRVERLCQAFENGRALVELSGNSPHDITSVLKRFLQELTDPVVPFHLYDAFISLAKTLHADPGDDPGTPNPSPEIIRSLKTLLVQLPDSNYSTLRHLVAHLFRVAARFEENKMSANNLGIVFGPTLLRPPDGPRATGASPVACLLDSGHQAQLVEFLIVHYEQIFG 757 HSP 2 Score: 58.5362 bits (140), Expect = 4.439e-7 Identity = 50/199 (25.13%), Postives = 94/199 (47.24%), Query Frame = 2
Query: 92 NPLMSGDQDTENKLSADTITEAS------LDLSP----GITEDD-KLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKL-LDVINRQ-----------RTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
+P+ D + + +A +TE + DLSP +TE++ L + K VD +L YAK W ++ KEL+ +K+A E+E +S K ++ + ++ L S++ + + ++++ LG L L+ + +Q R E K RK +KE+W + K+M +A + +++ Y QR
Sbjct: 43 DPVRREDLEPDKADTATVVTEENSEASSWRDLSPEGPAPLTEEELDLRLIRTKGGVDAALEYAKAWSRYAKELLAWTDKRANYELEFAKSIMKLAEAGKVSI--------------LQQSQMPLQYIYTLFLEHDLSLGALALETVAQQKRDYYQPLAAKRMEIEKWRKEFKEQWLKEQKRMNEAVQALRRSELQYIQR 227
BLAST of Rho-GAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9P107|GMIP_HUMAN (GEM-interacting protein OS=Homo sapiens OX=9606 GN=GMIP PE=1 SV=2) HSP 1 Score: 109.383 bits (272), Expect = 1.101e-22 Identity = 64/195 (32.82%), Postives = 107/195 (54.87%), Query Frame = 2
Query: 1655 VTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK--EAESGE-------VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSK--PRFRMA-DIKEYMDNKSQAKIVQIIIENVETLFG 2203
V +V KC IE L GIYRVS S+++V + ++ + A +L P +++V+K L L+EP++P+HLY+ F+ L K A+ G+ +++ L+ L+ QLP SN + HL ++A EN+M + NLG +F PTL++ PR A + +D+ QA++V+ +I + E +FG
Sbjct: 564 VPFVVTKCTAEIEHRALDVQGIYRVSGSRVRVERLCQAFENGRALVELSGNSPHDVSSVLKRFLQELTEPVIPFHLYDAFISLAKTLHADPGDDPGTPSPSPEVIRSLKTLLVQLPDSNYNTLRHLVAHLFRVAARFMENKMSANNLGIVFGPTLLRPPDGPRAASAIPVTCLLDSGHQAQLVEFLIVHYEQIFG 758 HSP 2 Score: 63.929 bits (154), Expect = 9.360e-9 Identity = 49/195 (25.13%), Postives = 88/195 (45.13%), Query Frame = 2
Query: 92 NPLMSGDQDTENKLSADTITEASLDLSPG-------ITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKL-LDVINRQ-----------RTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
+PL+S D + + +A EAS P E+ L + K VD +L YAK W ++ KEL+ EK+A+ E+E +S K ++ + ++ S + + + ++++ LG L ++ + +Q RTE K RK +KE+W + K+M +A + + + Y QR
Sbjct: 43 DPLLSEDPEPDKTPTATVTNEASCWSGPSPEGPVPLTGEELDLRLIRTKGGVDAALEYAKTWSRYAKELLAWTEKRASYELEFAKSTMKIAEAGKVSIQQQ--------------SHMPLQYIYTLFLEHDLSLGTLAMETVAQQKRDYYQPLAAKRTEIEKWRKEFKEQWMKEQKRMNEAVQALRRAQLQYVQR 223
BLAST of Rho-GAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5PQJ5|RHG29_RAT (Rho GTPase-activating protein 29 OS=Rattus norvegicus OX=10116 GN=Arhgap29 PE=1 SV=2) HSP 1 Score: 105.531 bits (262), Expect = 2.094e-21 Identity = 62/209 (29.67%), Postives = 105/209 (50.24%), Query Frame = 2
Query: 1637 QKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAE---------------------SGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+KE D + ++ C IE L GIYRV +K+K + ++L + D+ + +V+K L L EP + + LY EF+DL KE + S E++ ++ + + L+ QLP+SN + HLK++ +++EEN+M S NLG +F PTLI+ +P I + SQA++V+ +I + +F
Sbjct: 675 KKEPDGIPFVLKICASEIESRALCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEIQHVNEEQEAKKDSPEDKKHPHVSIEINRILLRSKDLLRQLPASNFNSLHYLIVHLKRVVDHAEENKMNSKNLGVIFGPTLIRPRPTTAPVTISSLAEYSSQARVVEFLITYAQKIF 883
BLAST of Rho-GAP domain-containing protein vs. UniProt/SwissProt
Match: sp|A7YY57|RHG29_BOVIN (Rho GTPase-activating protein 29 OS=Bos taurus OX=9913 GN=ARHGAP29 PE=2 SV=1) HSP 1 Score: 104.375 bits (259), Expect = 5.110e-21 Identity = 63/209 (30.14%), Postives = 105/209 (50.24%), Query Frame = 2
Query: 1637 QKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAE---------------------SGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+KE D + ++ C IE LS GIYRV +K+K + ++L + D+ + +V+K L L EP + + LY EF+DL E + S E+S ++ K + L+ QLP+SN + HLK++ ++SEEN+M S NLG +F P+L++ +P I D +QA++V+ +I + +F
Sbjct: 678 KKEPDGIPFVLKMCASEIENRALSLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAVEIQHVNEEQEMKKDNPEDKKWPSSSIEISRILLKSKDLLRQLPASNFNSLHYLIVHLKRVVDHSEENKMNSRNLGVIFGPSLLRPRPTTAPITISSLADYSNQARLVEFLITYSQKIF 886 HSP 2 Score: 57.3806 bits (137), Expect = 9.148e-7 Identity = 49/152 (32.24%), Postives = 73/152 (48.03%), Query Frame = 2
Query: 185 EDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIID-------QCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
E D +L K+ N+V+ +L+YAK W K+ K +V EKK LE+E R+ K ++ T + M +SL + L+ D Q +Q NK + LL ++ E K RK KE WK + KM++ E + K K QR
Sbjct: 193 ELDSMLL-KNTNSVELALSYAKTWSKYTKNIVSWVEKKLNLELESTRNIVKLAEATRTNIGLQEF----MPLQSLFTNALLNDIESSHLLQQTIAALQANKFVQPLLG----RKNEMEKQRKEIKELWKQEQNKMLETETALKKAKLLCMQR 335
BLAST of Rho-GAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6PCS4|RHG29_DANRE (Rho GTPase-activating protein 29 OS=Danio rerio OX=7955 GN=arhgap29 PE=2 SV=1) HSP 1 Score: 103.605 bits (257), Expect = 7.761e-21 Identity = 62/205 (30.24%), Postives = 109/205 (53.17%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAE-------------------SGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADI--KEYMDNKSQAKIVQIIIENVETLF 2200
D + I+ KC IE L+ GIYRV+ +K +V + ++ + +L + P ++NV+K L L EPL+ Y YN+ + L KE + S E+ ++ K++ L+ QLP+ + K + HL +++ +EEN+M + NLG +F PTLI KPR A++ +D QA++V+++I++ + +F
Sbjct: 748 DGIPFIIKKCTSEIESRALTIKGIYRVNGAKSRVEKLCQAFENGKDLVELSDLHPHDISNVLKLYLRQLPEPLILYRYYNDVIGLAKETQNMDKTDSAKEKSAGEQLGLSTELKRVLFKVRDLLRQLPAPHYKTLQFLITHLHRVSEQAEENKMTASNLGIIFGPTLI--KPRHLEAEVSLSSLVDYPHQARMVELLIKHHQMIF 950 HSP 2 Score: 56.6102 bits (135), Expect = 1.657e-6 Identity = 48/170 (28.24%), Postives = 85/170 (50.00%), Query Frame = 2
Query: 155 ASLDLSPGIT--EDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEI-----EIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLR 643
A LSP ++ E LL K+ + V+ +L YAK W K+ K+++ EK+ +L++E +S++K +S + S P R +S K I+ + +Q NK LL ++ + K RK KE+W+ + KKM ++E+ + K + Q+ ++ K R
Sbjct: 213 ADHSLSPELSTVEQVDLLLLKNDSGVESALLYAKAWSKYTKDVLAWVEKRLSLDMECAKSFAKMAESAKAVASQQDFM--PFRDIYVSAFKNEIEYNHVLLQTAAALQTNKFTQPLLA----RKNDLDKQRKEIKEQWQRELKKMNESESALKKARLLKMQKREEYEKAR 376
BLAST of Rho-GAP domain-containing protein vs. TrEMBL
Match: A0A4E0R5Y1 (RhoGAP OS=Fasciola hepatica OX=6192 GN=D915_005491 PE=4 SV=1) HSP 1 Score: 539.265 bits (1388), Expect = 5.034e-168 Identity = 311/737 (42.20%), Postives = 445/737 (60.38%), Query Frame = 2
Query: 125 NKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGS--------------TNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXX------XXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPXXXXXXXXXXXXXXXXGVPAS----SNRTGVLNTIVEFGSNLFRXXXXXXXXXXXXXXXRRNQQNKNKKXXXXXXXXXXXXXXXXAEKDLE-WQKEVDAVTEIVLKCIEVIE--INGLSTHGIYRVSASKLKVGSVMESLNS-------QGAD--ADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK---EAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQY 2218
+K S I E ++ G++E+DKLL RKDKNA+DF+ NY+KLWCK CK+LV+ EK+A E E CR+ K FQ+FET ++S + GAPM+++ LSL++++ID CKE E N K+ +LL+V+ RQRT F KTRK K+KWKSD K+M+DAEN+++KTKSAYYQRCQ GVKLREEL AQ L M TGS + Q S +GSTA+++ + ++ + S +++Q++KQ+ KV++LEKQL+DNDKKEIEQMY+Y+E+VE AN++L E EK KME LCD RLT +SDEVVKD+LAE L TR M+ +Y ++ FQ+Y PC +YR LIE+ + D Y EK+Q +H+ +++ K DS T R R IGS ++ T + G S S + VL+++VEFGSNLFR S S +R Q + + + + S T + L +KE A + +C+ IE GL THGIYRV ASK KV S++ES+ S + +D A L E P+TLA ++K++LI+L EPL+ Y+LY FV+LGK +++ + +LV +LQLL+N L N++LAGL+FHHL ++A ENQM S NLGT+F PT+++ KP+F++A++ E+MDNK Q ++V+++I+ V +FGP + Y
Sbjct: 54 SKSSVPVIKEFEIEFGHGVSEEDKLLSRKDKNAIDFAFNYSKLWCKSCKDLVLGLEKRAYAENEACRAIVKHFQAFETNLNS--LVGAPMKTQRLSLARMLIDHCKETETHNTKRCRELLEVLTRQRTLFEKTRKFLKDKWKSDVKRMLDAENSLIKTKSAYYQRCQTGVKLREELAMAQNQLNEMNAGMPVVTGSGPGIPSGSISPGPTSPGQGQSAPITNSPMGANQGQTVGSTASLTQDIADSAPDSAATSTSSTSNQVAKQRAKVERLEKQLSDNDKKEIEQMYAYREAVEQANHRLCELEKSKMEILCDTRLTIRKSDEVVKDSLAELFNHLYTTRINMVKQYETIAAAFQDYVPCSEYRALIEEHIQKGVDTYPEKYQFAGFHEASSSGGKL--------DSLTGRFSGR-SIGSARDASDNDPLAGTRSALCIHSDSDSEGEDEPGPSKSGGLVSTSSNVLSSLVEFGSNLFR------PDSKKSVRGKRGQGSAGSTNQAVMSGSATTESLDTLTAQLANLEKEYVAACYPIARCVAAIEKQPGGLETHGIYRVPASKAKVASLLESIQSSQPMTPEKASDDIAMLSQEHPLTLAGLIKTRLIALPEPLLTYNLYPNFVELGKIFDASDANILKELVKRLQLLINHLSPGNKRLAGLLFHHLYRVAACQSENQMSSANLGTMFGPTVLRQKPKFQVANMMEFMDNKGQTRVVEVLIDQVVEIFGPAEHY 773
BLAST of Rho-GAP domain-containing protein vs. TrEMBL
Match: A0A5J4NSN3 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0007433 PE=4 SV=1) HSP 1 Score: 523.857 bits (1348), Expect = 4.312e-162 Identity = 303/756 (40.08%), Postives = 451/756 (59.66%), Query Frame = 2
Query: 152 EASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDH-------------GIQET--GSTNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXX-------XXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEK------HKFGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPXXXXXXXXXXXXXXXXGVPASSNRTGVLNTIVEFGSNLFRXXXXXXXXXXXXXXXRRNQQNKNKKXXXXXXXXXXXXXXXXAEKDLEWQ-----KEVDAVTEIVLKCIEVIE--INGLSTHGIYRVSASKLKVGSVMESLNS-------QGAD--ADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK---EAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQ 2278
E ++ G++E+DK L R+DKNA++F+ NY+KLWCK CK++V+ EK+A E E CR+ KQFQ+ E +V++ + GAPM+ ++L ++D CKE+E N K+ +LL+V+ RQRT F KTRK+ K+KWK++ K+M+DAEN ++KTKS YYQRCQ GVKLREEL AQ LL + GI T G++N ++ + T+ S+ ++ GS ++ + S A + +++Q++KQK KV++LEKQL+DNDKKEIEQMY+Y+ESVE AN++L + EK KME LCDIRLT +SDEVVKD+LAE L TR M+++Y +++ FQ+Y PC DY+ L+E + D + EK+Q +H+ A + K G + S+ I S E+D E + N + + S + VL+++VEFGS+LFR + ++ + +T+ ++ + A +L Q +E + V +C+ IE GL THGIYRV ASK KV S++E+L S Q AD A L E P+TLA ++K++LI+L EPL+ Y LY FV+LGK A+ G V +LV +L+LL+N L N KLAGL+FHHL ++A ENQM NLGT+F PT+++ KP+F++A++ E+MDNK Q ++V+++IE V +FGP DQ Y+P T LA+ +T +++
Sbjct: 57 EFEIEFGHGVSEEDKSLSRRDKNAIEFAFNYSKLWCKSCKDIVLGLEKRAYAENEACRAIVKQFQALEASVAT--VNGAPMKQQTLMFLFQMVDHCKEMETHNTKRCRELLEVLTRQRTLFEKTRKYLKDKWKAEVKRMLDAENVLIKTKSGYYQRCQTGVKLREELAAAQNLLNELSASMSNIPSNAGTLQPGISSTLSGASNMSSTLPTTAVSSNNSGVSQTAGSAVSLVQEGADVSTDVNPTLAGTATSTQVAKQKAKVERLEKQLSDNDKKEIEQMYAYRESVETANSRLYDLEKTKMEILCDIRLTIMKSDEVVKDSLAELFNHLHTTRMAMISQYQTVASAFQDYVPCSDYKSLLEDHIQKGVDYFPEKYQFAGFHEAAASGLKLDALPGRFLGRTAATCSKEAGESDPLCIHSDSESDGEPQVENN-----------------TNLSSKSTGSTVLSSLVEFGSSLFRPDSKKSI----------RKRGQGSTTTAVVSTGIPVSATSDALDNLSTQLANLEREYISACYPVARCVSAIEKQPGGLETHGIYRVPASKAKVASLLETLQSSQPSSPEQIADDIAVLSQEHPLTLAGLIKTRLIALPEPLLTYSLYPNFVELGKVYDSADPGVVKELVKRLRLLINHLSPGNLKLAGLLFHHLHRVAACQSENQMSPANLGTMFGPTVLRQKPKFQVANMMEFMDNKGQTRVVEVLIECVTEIFGPADQ-YDPIRTLISLANSNDTPLER 782
BLAST of Rho-GAP domain-containing protein vs. TrEMBL
Match: A0A3R7C423 (Minor histocompatibility protein HA-1 (Fragment) OS=Clonorchis sinensis OX=79923 GN=HMHA1 PE=4 SV=1) HSP 1 Score: 511.531 bits (1316), Expect = 8.099e-159 Identity = 297/742 (40.03%), Postives = 451/742 (60.78%), Query Frame = 2
Query: 176 GITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNAT--------AQIXXXXXXXXXXXXXDGIGXXXX---------XXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHK------FGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPXXXXXXXXXXXXXXXXGVPASSNRTG--VLNTIVEFGSNLFRXXXXXXXXXXXXXXXRRNQQNKNKKXXXXXXXXXXXXXXXXAE-------KDLEWQKEVDAVTEIVLKCIEVIE--INGLSTHGIYRVSASKLKVGSVME-------SLNSQGAD--ADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK---EAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEE 2263
G++E+DK L R+ +NAV+F+ +Y+KLWCK CK++V+ EK+A E E RS +KQFQ+ E +VS+ + GAPM+ E++ L+ ++++ KE+E+ N K+ +LL+V+ RQRT F +TRK+ K+KWK+D K+++DAEN++ KTK+AYYQRCQ GVKLREEL AQ + Y + + T +T A+ A + +SN +++SN N GS + S T+ A +++Q++KQ+ KV++LEKQL+DNDKKEIE MY+Y+E+V+ ANN+L + EK KME LCD RLT +SDEVVKD+LAE L TR M+ +Y ++ FQEY PC DYR L+ +Q+ D Y EK+Q +H+ + K + G S + + G GI S +++ E +++P G P TG VL++IVEFGS+LFR ++++ + K + ++ ++ AE + +KE +A + +CI IE GL THGIYR+ ASK KV S++E S Q AD A L E P+TLA ++K++LI+L EPL+ Y+LY FV+LGK A+ V +LV +LQLL+ L NR+LAGL+FHHL ++A + ENQM S NLGT+FAPT+++ +P+F++A++ E++DN+ Q ++V+++I+ V +FGP + Y+P+ LA+ EE
Sbjct: 2 GVSEEDKALSRRHRNAVEFAFDYSKLWCKSCKDIVLGLEKRAYAENEAVRSIAKQFQALEASVSA--VNGAPMKQETIRLAHMMVEHSKEVELHNTKRCRELLEVLTRQRTNFERTRKYLKDKWKTDFKRLVDAENSLFKTKAAYYQRCQTGVKLREELAAAQ--IMYDEISTLSATSATPASTTSAAQNAAGVASSNAASSSNSNTQTAGSAPNLAQESEIPITTDVSLTSGAGGSTSTQLAKQRAKVERLEKQLSDNDKKEIELMYAYREAVDVANNRLYDLEKSKMEILCDTRLTILKSDEVVKDSLAELFNHLYTTRIAMVKQYETIAATFQEYVPCSDYRALVAEQIQKGVDSYPEKYQFAGFHEATASGLKLESTSGRLGGRISASSREASDGDPLSGIHSDSDSETEQPTDSSMP--------------PGGKPTGIVSTGSNVLSSIVEFGSSLFR------------PDSKKSIRGKRSQPSAPTSQVGIVPGPATAESLDNLTNQTNNLEKEYNAACIPLARCIAAIEKQPGGLETHGIYRIPASKAKVASLLEMIQTSHPSTAGQLADDIAVLSQENPLTLAGLIKTRLIALPEPLLTYNLYPNFVELGKVFDTADEKGVKELVKRLQLLITHLTPGNRRLAGLLFHHLNRVAASQAENQMNSANLGTMFAPTVLRQRPKFQVANMMEFVDNRGQTRVVEVLIDRVTDIFGPAAE-YDPRKIILVLANPEE 712
BLAST of Rho-GAP domain-containing protein vs. TrEMBL
Match: A0A4S2MGP8 (Rho-GAP domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001012 PE=4 SV=1) HSP 1 Score: 512.686 bits (1319), Expect = 1.082e-158 Identity = 305/761 (40.08%), Postives = 457/761 (60.05%), Query Frame = 2
Query: 92 NPLMSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQAL------LTYMQDHGIQETGSTNATAQIXXXXXXXXXXXXXDGIGXXXX---------XXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPXXXXXXXXXXXXXXXXGVPASSNRTG-------VLNTIVEFGSNLFRXXXXXXXXXXXXXXXRRNQQNKNKKXXXXXXXXXXXXXXXXAEKDLEWQKEVDAVTEIVLKCIEVIE--INGLSTHGIYRVSASKLKVGSVME-------SLNSQGAD--ADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGK---EAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEET 2266
NPL GD T+ K A+ E +++ G++E+DK L R+ ++ V+F+ +Y+KLWCK CK++V+ EK+A E E RS +KQF + E +VS+ + GAPM+ E++ L+ ++++ KE+E+ N K+ +LL+V+ RQRT F +TRK+ K+KWK+D K+++DAEN++ KTK+AYYQRCQ GVKLREEL AQ + L+ T + T + +SN +++SN N GS + SST+ A +++Q++KQ+ KV++LEKQL+DNDKKEIE MY+Y+E+V+ ANN+L + EK KME LCD RLT +SDEVVKD+LAE L TR M+ +Y ++ FQEY PC DYR L+ +Q+ D Y EK+Q +H+ + K ++T G R G G I + E+ + + L PT +P S TG VL++IVEFGS+LFR S R ++ + S+ T + + +KE +A + +CI IE GL THGIYR+ ASK KV S++E S Q AD A L E P+TLA ++K++LI+L EPL+ Y+LY FV+LGK A+ V +LV +LQLL+ L NR+LAGL+FHHL ++A + ENQM S NLGT+FAPT+++ +P+F++A++ E++DN+ Q ++V+++I+ V +FGP + Y+P+ LA+ EE+
Sbjct: 32 NPL--GDSVTQEK--ANAPKEFAIEFGHGVSEEDKALSRRHRHPVEFAFDYSKLWCKSCKDIVLGLEKRAYAENEAVRSIAKQFLALEASVSA--VNGAPMKQETIRLAHMMVEHSKEVELHNTKRCRELLEVLTRQRTNFERTRKYLKDKWKTDFKRLVDAENSLFKTKAAYYQRCQTGVKLREELAAAQIMYDEINTLSATSAAPTSNTSAAQNTTGVASSNPASSSNSNTQTAGSAPNLAPESEVPITADVSSTSGAGGSTSTQLAKQRAKVERLEKQLSDNDKKEIELMYAYREAVDVANNRLYDLEKSKMEILCDTRLTILKSDEVVKDSLAELFNHLYTTRIAMVKQYETIAAAFQEYVPCSDYRALVAEQIQKGVDSYPEKYQFAGFHEATASGLKL----------ESTSG--RLG-GRISASSREASDGDPLSGIHSDSDSETEQPTDSSMPPGSKPTGIVSTGSNVLSSIVEFGSSLFR-----PDSKKSIRGKRSQPSAPTSQAGTVPGSATTESLDNLTNQTNNLEKEYNAACIPLARCIAAIEKQPGGLETHGIYRIPASKAKVASLLEVIQTSHPSTAGQLADDIAVLSQENPLTLAGLIKTRLIALPEPLLTYNLYPNFVELGKVFDTADEKGVKELVKRLQLLITHLTPGNRRLAGLLFHHLNRVAASQAENQMSSANLGTMFAPTVLRQRPKFQVANMMEFVDNRGQTRVVEVLIDRVTDIFGPAAE-YDPRKIILALANPEES 767
BLAST of Rho-GAP domain-containing protein vs. TrEMBL
Match: A0A183A9L6 (Rho-GAP domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS3651 PE=4 SV=1) HSP 1 Score: 457.603 bits (1176), Expect = 1.380e-140 Identity = 297/770 (38.57%), Postives = 440/770 (57.14%), Query Frame = 2
Query: 176 GITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQ-------------------DHGIQETGSTNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTTRGLTRFGIGSIKET---DHESRNRNTLPXXXXXXXXXXXX-XXXXGVPASSNRTGVLNTIVEFGSNLFRXXXXXXXXXXXXXXXRRNQQNKNKKXXXXXXXXXXXXXXXXAEKDLE-WQKEVDAVTEIVLKCIEVIE--INGLSTHGIYRVSASKLKVGSVMESLNS-------QGAD--ADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNP--KLTKGDLADFEETKIQQEKDSLNVLKVCSEPAVDSKVKQQRPHSSIIES 2374
G++E+DKLL RKDKNA+DF+ NY+KLWCK CK+LV+ EK+A E E CR+ K FQ+FET ++S + GAPM++++LSL+++++D CKE E+ N K+ +LL+V+ RQRT F KTRK K+KWK+D K+M+DAEN+++KTKSAYYQRCQ GVKLREEL AQ L M G +G T + ++ + + + + T SA ST ++ S +++Q++KQ+ KV++LEKQL+DNDKKEIEQMY+Y+E+VE AN++L E EK KME LCD RLT +SDEVVKD+LAE L TR +M+ +Y ++ FQ+Y PC +YR LI++ + + Y EK+Q +H+ + + K D+ T R + R GS K+ D + R+ L HSDSDS+ E G S + VL+++VEFGSNLFR S S +RNQ + + + + S T + L +KE A + +C+ IE GL THGIYRV ASK KV S++ES+ S + +D A L E P+TLA ++K++LI++ + +++ + +LV +LQLL+N L N++LAGL+FHHL RF++A++ E+MDNK Q ++V+++I+ V +FGP +Q Y+P LT A +E +++ L SE + D+K P ++ +S
Sbjct: 16 GVSEEDKLLSRKDKNAIDFAFNYSKLWCKSCKDLVLGLEKRAYAENEACRAIVKHFQAFETNLNS--VNGAPMKTQTLSLARMMVDHCKETEMHNTKRCRELLEVLTRQRTLFEKTRKFLKDKWKADVKRMLDAENSLIKTKSAYYQRCQTGVKLREELAMAQNQLNEMNAGLVASGLGSGSTSGPVSPGPGSVASGQTTSLVNTSSGTTQSQTVGSTASLTQDITDSAPDSTATSSSSTSNQVAKQRAKVERLEKQLSDNDKKEIEQMYAYREAVEQANHRLCELEKSKMEILCDTRLTIRKSDEVVKDSLAELFNHLYATRISMIKQYESMATAFQDYVPCSEYRALIDEHIQKGVETYPEKYQFAGFHEASASGGKL--------DAVTGRFMGR-SAGSAKDASDNDPLAGTRSALCIHSDSDSEGEEDPGPSRGGGLVSTSSNVLSSLVEFGSNLFR------PDSKKSVRGKRNQGSGGSGNQTAMSGSATTESLDTLTAQLANLEKEYIAACYPIARCVAAIEKQPGGLETHGIYRVPASKAKVASLLESVQSSQPMTPEKASDDIAMLSQEHPLTLAGLIKTRLIAVFDA----------------SDANILKELVKRLQLLINHLSPGNKRLAGLLFHHLY-----------------------------RFQVANMMEFMDNKGQTRVVEVLIDQVVEIFGPAEQ-YDPILILTSAPPAAEDELDLRRGSGPLARRNTASEKSTDTKPTLLHPQAARAQS 722
BLAST of Rho-GAP domain-containing protein vs. Ensembl Cavefish
Match: arhgap29a (Rho GTPase activating protein 29a [Source:ZFIN;Acc:ZDB-GENE-030131-9510]) HSP 1 Score: 115.161 bits (287), Expect = 2.209e-25 Identity = 66/189 (34.92%), Postives = 110/189 (58.20%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAES---GEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADI--KEYMDNKSQAKIVQIIIENVETLF 2200
D + I+ KC IE L+ GIYRV+ +K +V + ++ + +L + P ++NV+K L L EPL+ + YN+F+ L KE +S E++ ++ K++ L+ QLP+++ K L+ HL ++ +EEN+M + NLG +F PTLI KPR AD+ +D QA IV+++I + E +F
Sbjct: 688 DGIPFIIRKCTTEIENRALNIKGIYRVNGAKSRVEKLCQAFENGKDLVELSDLYPHDISNVLKLYLRQLPEPLILFRYYNDFIGLAKECQSIIIDELNRVLFKIKDLLRQLPTTHYKTLQLLIRHLHRVTEKAEENKMTASNLGIIFGPTLI--KPRQADADVSLSSLVDYPYQALIVELLIRHYEMIF 874 HSP 2 Score: 51.6026 bits (122), Expect = 6.728e-6 Identity = 45/167 (26.95%), Postives = 85/167 (50.90%), Query Frame = 2
Query: 179 ITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIE-----IQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQ 664
+ E D +L + D + V+ +L YAK W K+ KEL+ +K+ ++++E +S +K +S +T + R + K I+ + ++ +Q NK + LL ++ E K RK KE+W+ + KKM +AE+ + K + QR ++ + R + A+
Sbjct: 228 VEELDSVLQQSD-SGVESALLYAKAWSKYTKELLNWVDKRLSMDLECAKSLAKMAESAKTLANQQEHMA--FRDIYTTAFKNDIEYSQVLQHTVSALQANKFMQPLLA----RKNELDKLRKEVKEQWQREQKKMHEAESALKKARLLKDQRQEEYERARISTSRAE 387
BLAST of Rho-GAP domain-containing protein vs. Ensembl Cavefish
Match: gmip (GEM interacting protein [Source:NCBI gene;Acc:103034590]) HSP 1 Score: 108.997 bits (271), Expect = 1.279e-23 Identity = 62/192 (32.29%), Postives = 104/192 (54.17%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKE----------AESGE-VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGP 2206
IV +C IE L+ G+YRVS SK ++ + + +Q DL P + +V+K L EPL+ + LYN+F+++GKE AE+ + V ++V ++ L+ +LP N + HL +I+ N EEN+M NLG +F PTL++ ++ I +++ QA +V+ +I N + +FGP
Sbjct: 570 IVQRCTGEIESRALTVQGVYRVSGSKPRILKLCHAFETQKDQVDLAEVSPHDVTSVLKYFFKELPEPLLTFDLYNDFINIGKEIQRLSEKDHVAETAQIVENIVINIKQLLARLPPCNYSTVRHMMGHLHRISENFEENKMSPGNLGIVFGPTLLRPLVSGDVSMIA-LLESSYQAMLVEFMITNHDQIFGP 760 HSP 2 Score: 67.3958 bits (163), Expect = 8.713e-11 Identity = 48/186 (25.81%), Postives = 92/186 (49.46%), Query Frame = 2
Query: 182 TEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNN---KKLGKLL-----DVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGST 715
+D L + ++ V+ +L YAK+WC++ K+L+ +K+ +LE E ++ +K ++ ++ V+ M P+ + I E +I+ + K +LL ++ +R E K R+ +KE+W + K+M DA + + + Y QRC EEL A+A+ G++E G +
Sbjct: 97 AQDADLAMCRSESGVELALQYAKMWCRYAKDLLTWLDKRISLEQEYAKNITKAAETAKSCVTQQDMM--PL--------QYIYTMAMEQDIKTSATAKHTTELLHQRCYQALSAKRNEIDKWRREFKEQWAKEQKRMNDAVAALKRARQQYLQRC-------EELEKAKAISA----KGLEEAGGS 261
BLAST of Rho-GAP domain-containing protein vs. Ensembl Cavefish
Match: gmip (GEM interacting protein [Source:NCBI gene;Acc:103034590]) HSP 1 Score: 108.997 bits (271), Expect = 1.328e-23 Identity = 62/192 (32.29%), Postives = 104/192 (54.17%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKE----------AESGE-VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGP 2206
IV +C IE L+ G+YRVS SK ++ + + +Q DL P + +V+K L EPL+ + LYN+F+++GKE AE+ + V ++V ++ L+ +LP N + HL +I+ N EEN+M NLG +F PTL++ ++ I +++ QA +V+ +I N + +FGP
Sbjct: 604 IVQRCTGEIESRALTVQGVYRVSGSKPRILKLCHAFETQKDQVDLAEVSPHDVTSVLKYFFKELPEPLLTFDLYNDFINIGKEIQRLSEKDHVAETAQIVENIVINIKQLLARLPPCNYSTVRHMMGHLHRISENFEENKMSPGNLGIVFGPTLLRPLVSGDVSMIA-LLESSYQAMLVEFMITNHDQIFGP 794 HSP 2 Score: 58.5362 bits (140), Expect = 4.477e-8 Identity = 48/210 (22.86%), Postives = 94/210 (44.76%), Query Frame = 2
Query: 182 TEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSV---------MFGAPMRSESLSL---------------SKLIIDQCKEIEIQNN---KKLGKLL-----DVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGST 715
+D L + ++ V+ +L YAK+WC++ K+L+ +K+ +LE E ++ +K ++ ++ V+ V + ++ E + I E +I+ + K +LL ++ +R E K R+ +KE+W + K+M DA + + + Y QRC EEL A+A+ G++E G +
Sbjct: 97 AQDADLAMCRSESGVELALQYAKMWCRYAKDLLTWLDKRISLEQEYAKNITKAAETAKSCVTQQVYEHTWVNVDVHAFALKCEETCFLVNDASCACVQDMMPLQYIYTMAMEQDIKTSATAKHTTELLHQRCYQALSAKRNEIDKWRREFKEQWAKEQKRMNDAVAALKRARQQYLQRC-------EELEKAKAI----SAKGLEEAGGS 295
BLAST of Rho-GAP domain-containing protein vs. Ensembl Cavefish
Match: gmip (GEM interacting protein [Source:NCBI gene;Acc:103034590]) HSP 1 Score: 108.612 bits (270), Expect = 1.889e-23 Identity = 63/195 (32.31%), Postives = 105/195 (53.85%), Query Frame = 2
Query: 1655 VTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKE----------AESGE-VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGP 2206
V IV +C IE L+ G+YRVS SK ++ + + +Q DL P + +V+K L EPL+ + LYN+F+++GKE AE+ + V ++V ++ L+ +LP N + HL +I+ N EEN+M NLG +F PTL++ ++ I +++ QA +V+ +I N + +FGP
Sbjct: 567 VPFIVQRCTGEIESRALTVQGVYRVSGSKPRILKLCHAFETQKDQVDLAEVSPHDVTSVLKYFFKELPEPLLTFDLYNDFINIGKEIQRLSEKDHVAETAQIVENIVINIKQLLARLPPCNYSTVRHMMGHLHRISENFEENKMSPGNLGIVFGPTLLRPLVSGDVSMIA-LLESSYQAMLVEFMITNHDQIFGP 760 HSP 2 Score: 68.9366 bits (167), Expect = 3.533e-11 Identity = 48/186 (25.81%), Postives = 92/186 (49.46%), Query Frame = 2
Query: 182 TEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNN---KKLGKLL-----DVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGST 715
+D L + ++ V+ +L YAK+WC++ K+L+ +K+ +LE E ++ +K ++ ++ V+ M P+ + I E +I+ + K +LL ++ +R E K R+ +KE+W + K+M DA + + + Y QRC EEL A+A+ G++E G +
Sbjct: 97 AQDADLAMCRSESGVELALQYAKMWCRYAKDLLTWLDKRISLEQEYAKNITKAAETAKSCVTQQDMM--PL--------QYIYTMAMEQDIKTSATAKHTTELLHQRCYQALSAKRNEIDKWRREFKEQWAKEQKRMNDAVAALKRARQQYLQRC-------EELEKAKAISA----KGLEEAGGS 261
BLAST of Rho-GAP domain-containing protein vs. Ensembl Cavefish
Match: arhgap29b (Rho GTPase activating protein 29b [Source:NCBI gene;Acc:378998]) HSP 1 Score: 107.842 bits (268), Expect = 4.211e-23 Identity = 65/205 (31.71%), Postives = 105/205 (51.22%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAE---------------------SGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + I+ KC IE LS GIYRV+ +K +V + ++ + +L + P ++NV+K L L EPL+ Y YNEF+ L KEA+ S E++ V K++ L+ QLP ++ + + HL ++ +EEN+M + NLG +F PTLIK + + +D QA +V+I I ++ T+F
Sbjct: 655 DGIPFIIKKCTSEIESRALSIKGIYRVNGAKSRVEKLCQAFENGKDLVELSDLYPHDISNVLKLYLRQLPEPLILYRYYNEFIGLAKEAQAILVEEAESARNRPLEEEPSLSMELNRTVFKIRDLLRQLPLAHYRTLHFLITHLHRVTECAEENKMTASNLGIIFGPTLIKPRQTEAEVSLSSLVDYPYQALMVEIFITHLATIF 859 HSP 2 Score: 54.6842 bits (130), Expect = 8.387e-7 Identity = 41/159 (25.79%), Postives = 84/159 (52.83%), Query Frame = 2
Query: 176 GITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFET-TVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKL--GKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLR 643
G+ + D+ L + D + V+ +L YAK W K+ K+++ EK+ +L++E +S++K +S + + M P R +S K ++ ++ +Q L + + + ++ + + RK KE+W+ + KKM +AE+ + K + QR ++ K R
Sbjct: 173 GVEKVDQALLKND-SGVESALLYAKAWSKYTKDVLAWVEKRLSLDMECAKSYAKMAESAKAVAIQQDYM---PFRDIYVSAFKNEMEH-SQLLLQTAAALQTNRFIQPLVARKNDLDRRRKELKEQWQREQKKMSEAESAVRKARLLQSQRREEYEKAR 326
BLAST of Rho-GAP domain-containing protein vs. Ensembl Sea Lamprey
Match: arhgap5 (Rho GTPase activating protein 5 [Source:ZFIN;Acc:ZDB-GENE-081014-1]) HSP 1 Score: 83.1889 bits (204), Expect = 3.610e-16 Identity = 64/189 (33.86%), Postives = 98/189 (51.85%), Query Frame = 2
Query: 1652 AVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADL----INEQPVTLANVVKSKLISLSEPLVPYHLYNEFVD-LGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFR-MADIKEYMDNKSQAKIVQIIIENVETLF 2200
AV + KC++ IE+NGL T G+YRVS +K ++ + L Q D I+ VT A +KS +L EPL+PY D +G E E D++T LQ +V +LP + + I HL ++ +++ N+M S NL F PTL+ KP F MA I + +++ +Q I + T+F
Sbjct: 1263 AVPVFIDKCVDFIEMNGLDTEGLYRVSGNKADQENI-QLLFGQDHTVDFRSLGISVHSVTGA--LKSFFTALPEPLIPYEQQKSLADAVGIENEK----DMITALQDVVKKLPQIHYDVFKFIITHLHRVYQHNQNNKMTSENLSICFWPTLM--KPNFTDMASITATLVSRTP---IQPFILHCPTIF 1439
BLAST of Rho-GAP domain-containing protein vs. Ensembl Sea Lamprey
Match: arhgap21b (Rho GTPase activating protein 21b [Source:ZFIN;Acc:ZDB-GENE-030131-4944]) HSP 1 Score: 78.9518 bits (193), Expect = 7.742e-15 Identity = 60/183 (32.79%), Postives = 95/183 (51.91%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLIS----LSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
IV C ++E GL + GIYRV + V S+ E LN + D +++ L NV+ S L S L EPL Y EF+D + E+G + + L+ L+++LP + + HLK IA NS +N+M NL +F PTL+++ D+ +M + Q KIV+ +I++ + F
Sbjct: 1025 IVEVCCGLVEERGLESTGIYRVPGNNAAVCSLQEELNRGVTEIDTNDDKWRDL-NVISSLLKSFFRKLPEPLFTDEKYYEFIDANR-MENG--VERLKALKKLIHELPEHHFHTLKFLSMHLKTIAENSPKNKMEPRNLAIVFGPTLVRTSE--DNTDMVTHMPD--QYKIVETLIQHYDWFF 1199
BLAST of Rho-GAP domain-containing protein vs. Ensembl Sea Lamprey
Match: arhgap5 (Rho GTPase activating protein 5 [Source:ZFIN;Acc:ZDB-GENE-081014-1]) HSP 1 Score: 74.7146 bits (182), Expect = 1.312e-13 Identity = 52/183 (28.42%), Postives = 89/183 (48.63%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNS-QGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLL---VNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
V KCI IE GL+ G+YRVS SK++ ++ + ++ Q D + +A +K+ L PL+PY ++ + E+ + D T+L L + +LP++N + + HL ++ NS N+M S NL F PTL+ +P F + D M + +I++ I +F
Sbjct: 1245 FVEKCITFIEDKGLTMEGVYRVSGSKVEQDNLQRTFDADQNIIFDNMELNVNAVAGALKAFFNELPGPLIPYDMHQGLI------EAITIPDKTTRLLALRGKLKKLPAANMAVLKCVITHLHTVSQNSSLNKMTSENLSICFWPTLM--RPDFTVVD--PVMATRVYKQILETFIHQCPFMF 1417
BLAST of Rho-GAP domain-containing protein vs. Ensembl Sea Lamprey
Match: chn2 (chimerin 2 [Source:ZFIN;Acc:ZDB-GENE-091020-5]) HSP 1 Score: 70.4774 bits (171), Expect = 3.797e-13 Identity = 55/187 (29.41%), Postives = 88/187 (47.06%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADL---INEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQL----PSSNRKLAGLIFH-HLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+V CI IE GL + G+YRVS V V + + +G AD+ E + +K L PLV Y Y FV E+ +VSD +L+ L L P+ L L+ H H ++++ + N M S NL +F PTL++ + +A + + Q +V+++I +V+ LF
Sbjct: 128 VVEMCIGEIEARGLKSEGLYRVSGVSDLVEEVRTAFDKEGEKADISAKAYEDVNVITGALKLYFRELPTPLVTYQAYPHFV------EAAKVSDPAERLEQLRRALQTLPPAHYDTLHCLMAHLHRRRVSQQEKHNLMSSENLSIVFGPTLMRPHEQDVLATLN---GMRHQRLLVELLIRHVQELF 305
BLAST of Rho-GAP domain-containing protein vs. Ensembl Sea Lamprey
Match: chn2 (chimerin 2 [Source:ZFIN;Acc:ZDB-GENE-091020-5]) HSP 1 Score: 68.1662 bits (165), Expect = 6.929e-13 Identity = 52/187 (27.81%), Postives = 91/187 (48.66%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADL-------INEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGE-VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
+V CI+ IE+ GL + G+YRVS + V + + G AD+ IN + +K L PL+ Y Y +F++ K ++ E + + L+LL P ++ + + HLK++ S+EN M + NL +F PTL++ + +A + D + Q +++ +I N E LF
Sbjct: 48 VVDMCIKEIEMRGLRSEGLYRVSGFSEHIEDVKMAFDRDGEKADISANSYDDIN----IITGALKLYFRDLPIPLITYDAYPKFIEASKLMDADERLEGIHEALRLL----PPAHYESLRYLMAHLKRVTLYSKENLMSAENLAIVFGPTLMRPPEQDVLATLN---DIRYQRLVIETLINNEEILF 223
BLAST of Rho-GAP domain-containing protein vs. Ensembl Yeast
Match: RGD1 (GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization [Source:SGD;Acc:S000000464]) HSP 1 Score: 55.8398 bits (133), Expect = 3.305e-8 Identity = 51/200 (25.50%), Postives = 92/200 (46.00%), Query Frame = 2
Query: 1643 EVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLI-------NEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTK---LQLLVNQLPSSN-RKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPE 2209
E D V IV +CI VI+ GL GIYR SA+ L V + E ++ A+ +I + + +++K+ SL + ++P L +E ++ D T+ + L+ LP + L L+F HLK++ + +N+M L ++ PT+ + P + D Q +++++E + F PE
Sbjct: 481 EQDMVPAIVRQCIYVIDKFGLDQEGIYRKSANVLDVSKLKEEIDKDPANISMILPSKPHSDSDIYLVGSLLKTFFASLPDSVLPKALSSEI------KVCLQIEDPTTRKNFMHGLIYNLPDAQYWTLRALVF-HLKRVLAHEAQNRMNLRALCIIWGPTIAPANP-------DDANDVNFQIMAMEVLLEVSDQAFEPE 666
BLAST of Rho-GAP domain-containing protein vs. Ensembl Yeast
Match: RGA2 (GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication [Source:SGD;Acc:S000002787]) HSP 1 Score: 52.7582 bits (125), Expect = 3.589e-7 Identity = 49/194 (25.26%), Postives = 89/194 (45.88%), Query Frame = 2
Query: 1700 GLSTHGIYRVSASKLKVGSVMESL---NSQGADA------DLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGE-----------------VSDLVTKLQLLVNQ-LPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
GL+ G+YR S S+ V + NS +D L+N+ +A+V+K L L +P++ + +Y+ +DL + + E + ++V K L + + LP ++++ ++ H+ K+ SE N M NL +FAP+LI K+ +D K + IV+ I+ N +F
Sbjct: 818 GLNMEGLYRKSGSQTLVEEIENEFAQNNSLHSDTLSPKLNALLNQDIHAVASVLKRYLRKLPDPVLSFSIYDALIDLVRNNQLIERLPLNNDKFLDSPQKVTIYEMVLKSLLEIFKILPVEHQEVLKVLAAHIGKVRRCSERNLMNLHNLSLVFAPSLIHD-----FDGEKDIVDMKERNYIVEFILGNYRDIF 1006
BLAST of Rho-GAP domain-containing protein vs. Ensembl Yeast
Match: BAG7 (Rho GTPase activating protein (RhoGAP); stimulates the intrinsic GTPase activity of Rho1p, which plays a bud growth by regulating actin cytoskeleton organization and cell wall biosynthesis, resulting in the downregulation of Rho1p; structurally and functionally related to Sac7p; BAG7 has a paralog, SAC7, that arose from the whole genome duplication [Source:SGD;Acc:S000005660]) HSP 1 Score: 51.9878 bits (123), Expect = 3.693e-7 Identity = 48/172 (27.91%), Postives = 83/172 (48.26%), Query Frame = 2
Query: 1643 EVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVT-----LANVVKSKLISLSEPLVPYHLYNEFVD--LGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEY 2137
E+ ++ ++ K + ++ N L T GI+R++ S +V ++++ S+ D E +A ++K L SLSEPLVP LY+ F + L + ++ + + LP NR L + L A N ++N M + NL + P+L+ S P+ M KEY
Sbjct: 71 EIGSIPVVIAKSGKYLKENALDTTGIFRIAGSNKRVRE-LQAVFSKPPDYGRKFEGWCDFNVHDIATLLKRYLNSLSEPLVPLALYDIFRNPILENPKINEHKEQIIKDYEDIYMLLPQQNRHLILYLAALLNLFARNEKKNLMSASNLAAIVQPSLL-SHPKDEMCP-KEY 239
BLAST of Rho-GAP domain-containing protein vs. Ensembl Yeast
Match: BEM2 (Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p [Source:SGD;Acc:S000000957]) HSP 1 Score: 52.7582 bits (125), Expect = 3.818e-7 Identity = 40/161 (24.84%), Postives = 77/161 (47.83%), Query Frame = 2
Query: 1637 QKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGA-DADLINE-----QPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQL--LVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLI 2095
++E + IV+K +E IE+ GL G+YR+ S + ++ + + +GA D E + +A K L L + L + + N+F DL + ++ + + K + L+ +LP+ + I HL K+ + N+M + NL +F+ + I
Sbjct: 1974 ERENTLIPTIVVKLLEEIELRGLDEVGLYRIPGSIGSINALKNAFDEEGATDNSFTLEDDRWFEVNAIAGCFKMYLRELPDSLFSHAMVNDFTDLAIKYKAHAMVNEEYKRMMNELLQKLPTCYYQTLKRIVFHLNKVHQHVVNNKMDASNLAIVFSMSFI 2134
BLAST of Rho-GAP domain-containing protein vs. Ensembl Yeast
Match: RGA1 (GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication [Source:SGD;Acc:S000005653]) HSP 1 Score: 50.447 bits (119), Expect = 1.855e-6 Identity = 48/212 (22.64%), Postives = 94/212 (44.34%), Query Frame = 2
Query: 1643 EVDAVTEIVLKCIEVIEIN--GLSTHGIYRVSASKLKVGSVMESLNSQGADAD-----LINEQPV-TLANVVKSKLISLSEPLVPYHLYNEFVDL--------------GKEAESGEVSDLV----TKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
E + + I+ CI+ IE + + + GIYR S S+L + + + ++ + ++ EQ + + V+K L L P+ + +Y + L GK + + SD + L+ ++ LP + ++ ++ H++K+ S N+M NL +FAP LI+ + K+ +D K + IV I N + +
Sbjct: 800 ENNEIPMILSVCIDFIESDEENMRSEGIYRKSGSQLVIEEIEKQFSAWKVQQNTETPNILTEQDLNVVTGVLKRYLRKLPNPIFTFQIYEPLMRLVKSKKMMENLPFVGGKLSLEAKNSDTYMSSKSALKNILEDLPREHYRVLRVLSEHIEKVTRYSHWNRMTLYNLALVFAPGLIRD-----FSGEKDIIDMKERNYIVAFIFGNYKDIL 1006
BLAST of Rho-GAP domain-containing protein vs. Ensembl Nematostella
Match: EDO45667 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RS04]) HSP 1 Score: 90.5077 bits (223), Expect = 2.799e-18 Identity = 59/191 (30.89%), Postives = 100/191 (52.36%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPV----------TLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGP 2206
+ K I +E GL+ G+YR+ + VGS +++L Q + I++ + T+ + +K L ++ PL Y + +F+D K+ V L LV LP N+KL +I HLK +A NS +N M + NLG +F PTL+KS+ MA I M+ K Q+ +++++++ + +FGP
Sbjct: 395 FIRKSIRAVEKRGLTDQGLYRI----VGVGSKVQNLLFQCVEKKKISQIELESDDSEYEVKTITSALKQHLRNMDPPLFTYEYHEDFLDAVKQ---DTYEKRVASLSKLVQLLPEENKKLIVMIMEHLKNVAANSNKNLMAASNLGVVFGPTLMKSREE-TMAAI---MNLKYQSVVIELLVKEFDRVFGP 574
BLAST of Rho-GAP domain-containing protein vs. Ensembl Nematostella
Match: EDO47441 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM51]) HSP 1 Score: 83.1889 bits (204), Expect = 4.568e-16 Identity = 65/189 (34.39%), Postives = 97/189 (51.32%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESL--NSQGADADLINEQPV-TLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFR--MADIKEYMDNKSQAKIVQIIIE-NVETLFGPED 2212
IV+ C+ IE GL GIYR+S + V + E NSQ A L++E + +A + K L L EPL LY++FV A++ E + T L+L LP+ NR A +F HL+K+A SE ++MG NL T+F P +++ P+ M + +D SQ I ++ + E L P D
Sbjct: 628 IVIGCVREIEKRGLEEVGIYRLSGASSDVKRLKEGFDENSQSALV-LVSEADIHAVAGLFKMYLRDLPEPLFTDELYDKFVKAYAMADAEEKRE--TMLELF-QSLPTPNRLTACYLFQHLRKVAEKSEVHKMGLNNLSTVFGPNVLRPSPKTSSPMDLAQGTLDVMSQVGIFMYFLKLDAEELHLPND 812
BLAST of Rho-GAP domain-containing protein vs. Ensembl Nematostella
Match: EDO46865 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNI7]) HSP 1 Score: 75.485 bits (184), Expect = 9.636e-14 Identity = 48/189 (25.40%), Postives = 100/189 (52.91%), Query Frame = 2
Query: 1655 VTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGAD--ADLINEQPV-TLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPED 2212
+ E+V C+ I+ GL GI+R+S S + M+ L G D A L + + + +A +++ L +PL P Y +F++ + + D++ ++ ++N+LP S ++ +F LK+++ +SE+N+M + NL +F PTL++ M + +++ ++++II+N + +F D
Sbjct: 441 IPEVVESCVRFIKRFGLDHPGIFRLSGSSTDIND-MKQLFECGRDPLAGLNHWKDINAVAGLLRVYFRELEDPLFPSSHYQQFIEASRFPTN---EDVIRQVTCVINELPDSVNRVMKYLFKFLKQVSEHSEQNKMDAHNLAVVFGPTLLRIPSEQDMITYQSHVNG-----MMEVIIKNYDQVFPQND 620
BLAST of Rho-GAP domain-containing protein vs. Ensembl Nematostella
Match: EDO49086 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RI38]) HSP 1 Score: 67.781 bits (164), Expect = 4.972e-13 Identity = 45/158 (28.48%), Postives = 84/158 (53.16%), Query Frame = 2
Query: 1718 IYRVSASKLKVGSVMESLNSQGADADLINEQPV----TLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIII 2179
+ RV + VG++ E LN +GA+ E+ + +++K L L E LV LY F++ ++ + E + L++L+N+LP N + + HLK +A +++ N+M + NL +F PTL+++ M+ +K+ D Q +IV+ I+
Sbjct: 4 VRRVPGNSAAVGALSEELNEKGAENINFQEEKWHDVNNITSLLKQFLRKLPEGLVTAELYESFIEANRKDDPVE---RMGALKMLINELPDHNYETLRHLLTHLKHVAEHADTNKMEARNLAIVFGPTLVRTGEDTMMSMVKDMSD---QCRIVETIL 155
BLAST of Rho-GAP domain-containing protein vs. Ensembl Nematostella
Match: EDO44981 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUA5]) HSP 1 Score: 72.4034 bits (176), Expect = 1.072e-12 Identity = 55/186 (29.57%), Postives = 88/186 (47.31%), Query Frame = 2
Query: 1664 IVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADL----INEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPE 2209
V KCI+ +E G+ GIYR S + ++ S + + DAD +N VT A +K L L EPL+P +L N +D ++ E + L+ LV QLP N + + HL ++ S+EN M S NL T+ PT+++++ ++ + +Q IE E +F E
Sbjct: 1540 FVEKCIDFVEAEGMQIEGIYRKSGKIEDIRTIQNSFDEK-RDADFSALEMNVHAVTGA--LKKFLKLLPEPLIPNNLQNNILDATVIMDADE---RLAVLRGLVMQLPKVNFAVLKYLMCHLNRVTEYSKENLMESRNLATVIFPTILRTE----FESLQTMARTMNYGLFIQTCIEKCEYMFDEE 1715
BLAST of Rho-GAP domain-containing protein vs. Ensembl Medaka
Match: gmip (GEM interacting protein [Source:NCBI gene;Acc:101164647]) HSP 1 Score: 108.997 bits (271), Expect = 1.845e-23 Identity = 62/199 (31.16%), Postives = 104/199 (52.26%), Query Frame = 2
Query: 1637 QKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAE----------SGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFG 2203
Q D V +V +C IE LS G+YRVS SK ++ + ++ Q DL + P + +++K L EPL+ + LYN+FV +GK + S E+ D+V LQ L+ +LP+ + I HL++++ N EN+M NLG +F PTL++ M+ I ++ Q+ +++ +I + T+FG
Sbjct: 537 QDTADEVPFVVTRCTSEIESRALSVQGVYRVSGSKPRIQKLCQAFEMQKEQVDLSDNSPHDITSMLKHFFKELPEPLLTFDLYNDFVAVGKAIQHLFEKETSPGSNEIMDIVQDLQKLLKRLPTHSYSTLQHIISHLQRVSEN-HENKMSPSNLGIVFGPTLLRPLVSADMSMIA-ILETSYQSMLIEFLITHYNTIFG 733 HSP 2 Score: 68.1662 bits (165), Expect = 5.380e-11 Identity = 42/144 (29.17%), Postives = 75/144 (52.08%), Query Frame = 2
Query: 215 KNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNN---KKLGKLLD-----VINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRC 622
++ V+ +L YAK+WC++ K+L+ EK+ +LE E ++ K + +T V M P+ + I + E +I+N+ +K +LL + +R E K R+ +KE+W + +KM DA + K + Y+QRC
Sbjct: 98 EDGVETALQYAKMWCRYAKDLLAWMEKRISLEQEFAKNVIKTAEGAKTNVWQQEMM--PL--------QYIYNMALEQDIKNSLTSRKTCELLQTRCYQALAAKRNEIDKWRREFKEQWAREQRKMNDAVTALKKARHQYFQRC 231
BLAST of Rho-GAP domain-containing protein vs. Ensembl Medaka
Match: arhgap42a (rho GTPase-activating protein 42 [Source:NCBI gene;Acc:101172099]) HSP 1 Score: 106.686 bits (265), Expect = 7.147e-23 Identity = 64/183 (34.97%), Postives = 107/183 (58.47%), Query Frame = 2
Query: 1673 KCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGA------DADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFG 2203
KCIE+IE G++T G+YR KV +M S+ + A D+D+ + + +T A +K L+EPL+ Y L+ +FV + A+ + S V + LV++LP N+ + L+ +HL K++++S++NQM NLG +F PTL++S+ + M+ K Q +V+IIIEN + +FG
Sbjct: 400 KCIELIEARGITTVGLYRTGGVNSKVQRLMTSVFASTATSEIQLDSDVWDNKTITSA--LKDYFRCLAEPLLTYRLHKDFV---RAAKFEDQSYRVRAIHALVHKLPEKNKAMLDLLTNHLLKVSSHSDQNQMTVSNLGMIFGPTLMRSQEE----TVAAMMNIKFQNIVVEIIIENHKKIFG 573
BLAST of Rho-GAP domain-containing protein vs. Ensembl Medaka
Match: arhgap45b (Rho GTPase activating protein 45 [Source:NCBI gene;Acc:101162675]) HSP 1 Score: 106.301 bits (264), Expect = 1.275e-22 Identity = 64/213 (30.05%), Postives = 101/213 (47.42%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGE-----------------------------VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + I+ KCI IE L GIYRV+ K +V + ++ + +L P ++NV+K L L EP++P+ LYN + + KE+ GE V LV L+ ++ +LP +N I HHL++IA EEN+M NLG +F P+LI+ +P + +D QA+IV+ +I ++F
Sbjct: 798 DCIPFIITKCISEIERRALKMKGIYRVNGVKTRVEKLCQAFENGKELVELSQCSPHDISNVLKLYLRQLPEPIMPFRLYNTLMGMAKESLHGEGEESEPSAINPAVSRGPELIDLGPDTDPDVLALVDSLKEILKELPKANFATLRYIIHHLRRIAELEEENKMSPSNLGIVFGPSLIRPRPTGATISLSSLVDYPHQARIVEALIVFYSSIF 1010 HSP 2 Score: 69.3218 bits (168), Expect = 2.379e-11 Identity = 82/379 (21.64%), Postives = 153/379 (40.37%), Query Frame = 2
Query: 176 GITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRS-ESLSLSKLIIDQCKEIEIQ---NNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEK-QLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTT 1297
G E+ ++ + + VD +L YAK K+ K+L+ EK+ +LE+E + K +QS + +++ M P+ S SL+L + DQ + + +Q N + + + +++ E K RK KE W +K+++ E + K K AY RC EE A+ ++ G TGST + + K +LE++ + K +E +Y+ + +A + +E E K+ L ++ +SD+ ++ Y +++ + Y L + Y P Y + QL +P ++ + E Y + + GH R+DS T
Sbjct: 308 GRVEEVDVILQHSEGGVDSALLYAKSTAKYMKDLISYVEKRTSLEMEFSKGLQKLYQSCKHSITHPHM---PLFSIYSLALEQ---DQEQSVGLQQASNTLQSQTFVQPLTQRKQEHEKKRKEIKENWFRARRKLLECEANLRKAKQAYMARC-------EEYEKAKTATCRAEEEG---TGSTAKSVE---------------------------------------------KKKRLEEEARN---KAVEAEATYRTCIADATTQQLELEHTKVTVLRQLQDIIKQSDQTLRSATISYYQLMHMQTVALPVHYQTLCESSKLYDPGQQYAAHVRDLQLPGQPTVH---YVFETY---SPSSSGTSHGHRPRNDSFNT 616
BLAST of Rho-GAP domain-containing protein vs. Ensembl Medaka
Match: arhgap45b (Rho GTPase activating protein 45 [Source:NCBI gene;Acc:101162675]) HSP 1 Score: 106.301 bits (264), Expect = 1.385e-22 Identity = 64/213 (30.05%), Postives = 101/213 (47.42%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGE-----------------------------VSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
D + I+ KCI IE L GIYRV+ K +V + ++ + +L P ++NV+K L L EP++P+ LYN + + KE+ GE V LV L+ ++ +LP +N I HHL++IA EEN+M NLG +F P+LI+ +P + +D QA+IV+ +I ++F
Sbjct: 846 DCIPFIITKCISEIERRALKMKGIYRVNGVKTRVEKLCQAFENGKELVELSQCSPHDISNVLKLYLRQLPEPIMPFRLYNTLMGMAKESLHGEGEESEPSAINPAVSRGPELIDLGPDTDPDVLALVDSLKEILKELPKANFATLRYIIHHLRRIAELEEENKMSPSNLGIVFGPSLIRPRPTGATISLSSLVDYPHQARIVEALIVFYSSIF 1058 HSP 2 Score: 69.707 bits (169), Expect = 1.837e-11 Identity = 82/379 (21.64%), Postives = 153/379 (40.37%), Query Frame = 2
Query: 176 GITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRS-ESLSLSKLIIDQCKEIEIQ---NNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEK-QLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTT 1297
G E+ ++ + + VD +L YAK K+ K+L+ EK+ +LE+E + K +QS + +++ M P+ S SL+L + DQ + + +Q N + + + +++ E K RK KE W +K+++ E + K K AY RC EE A+ ++ G TGST + + K +LE++ + K +E +Y+ + +A + +E E K+ L ++ +SD+ ++ Y +++ + Y L + Y P Y + QL +P ++ + E Y + + GH R+DS T
Sbjct: 356 GRVEEVDVILQHSEGGVDSALLYAKSTAKYMKDLISYVEKRTSLEMEFSKGLQKLYQSCKHSITHPHM---PLFSIYSLALEQ---DQEQSVGLQQASNTLQSQTFVQPLTQRKQEHEKKRKEIKENWFRARRKLLECEANLRKAKQAYMARC-------EEYEKAKTATCRAEEEG---TGSTAKSVE---------------------------------------------KKKRLEEEARN---KAVEAEATYRTCIADATTQQLELEHTKVTVLRQLQDIIKQSDQTLRSATISYYQLMHMQTVALPVHYQTLCESSKLYDPGQQYAAHVRDLQLPGQPTVH---YVFETY---SPSSSGTSHGHRPRNDSFNT 664
BLAST of Rho-GAP domain-containing protein vs. Ensembl Medaka
Match: arhgap29a (rho GTPase-activating protein 29 [Source:NCBI gene;Acc:101167748]) HSP 1 Score: 102.064 bits (253), Expect = 2.849e-21 Identity = 67/208 (32.21%), Postives = 106/208 (50.96%), Query Frame = 2
Query: 1649 DAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDL---------VTKLQL-------------LVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADI--KEYMDNKSQAKIVQIIIENVETLF 2200
D + I+ KC IE L+ GIYRV+ +K +V + ++ + +L P ++NV+K L L EPL+ + YN+F+ L KE++S V DL VT Q+ L+ LP ++ K + HL ++ SEEN+M + NLG +F PTL+ KPR AD+ +D QA IV+++I + + +F
Sbjct: 747 DGIPFIIRKCTSEIENRALNIKGIYRVNGAKSRVEKLCQAFENGKHLVELSELYPHDISNVLKLYLRQLPEPLILFRYYNDFIGLAKESQSIIVEDLEALRLNTNPVTPAQVSVDLNRVLFKMKDLLRHLPPAHYKTLQFLIEHLHRVTECSEENKMTASNLGIIFGPTLL--KPRQADADVSLSSLVDYPYQALIVELLIRHYQMVF 952 HSP 2 Score: 63.929 bits (154), Expect = 1.109e-9 Identity = 45/152 (29.61%), Postives = 80/152 (52.63%), Query Frame = 2
Query: 185 EDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLS-------LSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQR 619
E D+ L R+D + VD +L YAK W K+ KEL+ EK+ ++IE ++++K ++ + S P R ++ S+L+++ +Q+NK + LL ++ E + RK KE+W+ + KKM +A+N + K + QR
Sbjct: 228 EVDRALQRQD-SGVDSALLYAKAWSKYTKELLTWVEKRLNMDIECAKTYAKMAETAKGLASQQEFM--PFREIYMTAFKNDNEYSQLVLNTAA--ILQSNKFMQPLL----ARKNELDRRRKEVKEQWQREQKKMHEADNALKKARLLQTQR 370
BLAST of Rho-GAP domain-containing protein vs. Planmine SMEST
Match: SMESG000078332.1 (SMESG000078332.1) HSP 1 Score: 2022.28 bits (5238), Expect = 0.000e+0 Identity = 1126/1133 (99.38%), Postives = 1128/1133 (99.56%), Query Frame = 2
Query: 101 MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPXXXXXXXXXXXXXXXXGVPASSNRTGVLNTIVEFGSNLFRXXXXXXXXXXXXXXXRRNQQNKNKKXXXXXXXXXXXXXXXXAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCSEPAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVPASGHTVXXXXXXTPEKPAPKMMDMLFGXXXXXXXXXXXPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRXXXXXXXXXXXXXXXXXXXXPLIEHNIKSSNSMLXXXXXXXXXXXXYNYSSHLDSTSNSQNENTDSQSHLPFDPILETLFNSPLSNSKNCSHFSNVIPRKNLRYRSRSYIMSVEPHNKPSNRISSSVSASPFIRQSKIRVPLLAKSFSDVNNSSVTFFTEGDDNFNSKGSNDKSKLSMSSQYTKLNSFLLKYCSVDNIDKIDHSKVAKYQSWLSQPVFDEFFV 3499
MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQ NTSNQSNTSNVNVDGIGSTATISASSSTTSADSISASQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPSHSDSDSDTETTPTGDGVPASSNRTGVLNTIVEFGSNLFRQGGGGAQGSTSSDATRRNQQNKNKKSTSGSTSSMTGCTEGGAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCS PAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVP SGHTVSSSISSTPEKPAPKMMDMLFGSDTSTTIRSTSPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRSFISSTESSFVSKSNQNSSNPLIEHNIKSSNSMLSRSRPTSATTRSYNYSSHLDSTSNSQNENTDSQSHLPFDPI ETLFNSPLSNSKNCSHFSNVIPRKNLRYRSRSYIMSVEPHNKPSNRISSSVSASPFIRQSKIRVPLLAKSFSDVNNSSVT FTEGDDNFNSKGSNDKSKLSMSSQY+K+NSFLLKYCSVDNIDKIDHSKVAKYQSWLSQPVFDEFFV
Sbjct: 1 MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQTNTSNQSNTSNVNVDGIGSTATISASSSTTSADSISASQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPSHSDSDSDTETTPTGDGVPASSNRTGVLNTIVEFGSNLFRQGGGGAQGSTSSDATRRNQQNKNKKSTSGSTSSMTGCTEGGAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCSIPAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVPTSGHTVSSSISSTPEKPAPKMMDMLFGSDTSTTIRSTSPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRSFISSTESSFVSKSNQNSSNPLIEHNIKSSNSMLSRSRPTSATTRSYNYSSHLDSTSNSQNENTDSQSHLPFDPIPETLFNSPLSNSKNCSHFSNVIPRKNLRYRSRSYIMSVEPHNKPSNRISSSVSASPFIRQSKIRVPLLAKSFSDVNNSSVTIFTEGDDNFNSKGSNDKSKLSMSSQYSKMNSFLLKYCSVDNIDKIDHSKVAKYQSWLSQPVFDEFFV 1133
BLAST of Rho-GAP domain-containing protein vs. Planmine SMEST
Match: SMESG000078332.1 (SMESG000078332.1) HSP 1 Score: 1996.86 bits (5172), Expect = 0.000e+0 Identity = 1118/1133 (98.68%), Postives = 1120/1133 (98.85%), Query Frame = 2
Query: 101 MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPXXXXXXXXXXXXXXXXGVPASSNRTGVLNTIVEFGSNLFRXXXXXXXXXXXXXXXRRNQQNKNKKXXXXXXXXXXXXXXXXAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCSEPAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVPASGHTVXXXXXXTPEKPAPKMMDMLFGXXXXXXXXXXXPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRXXXXXXXXXXXXXXXXXXXXPLIEHNIKSSNSMLXXXXXXXXXXXXYNYSSHLDSTSNSQNENTDSQSHLPFDPILETLFNSPLSNSKNCSHFSNVIPRKNLRYRSRSYIMSVEPHNKPSNRISSSVSASPFIRQSKIRVPLLAKSFSDVNNSSVTFFTEGDDNFNSKGSNDKSKLSMSSQYTKLNSFLLKYCSVDNIDKIDHSKVAKYQSWLSQPVFDEFFV 3499
MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQ NTSNQSNTSNVNVDGIGSTATISASSSTTSADSISASQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEK SDSQTTRGLTRFGIGSIKETDHESRNRNTLPSHSDSDSDTETTPTGDGVPASSNRTGVLNTIVEFGSNLFRQGGGGAQGSTSSDATRRNQQNKNKKSTSGSTSSMTGCTEGGAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCS PAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVP SGHTVSSSISSTPEKPAPKMMDMLFGSDTSTTIRSTSPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRSFISSTESSFVSKSNQNSSNPLIEHNIKSSNSMLSRSRPTSATTRSYNYSSHLDSTSNSQNENTDSQSHLPFDPI ETLFNSPLSNSKNCSHFSNVIPRKNLRYRSRSYIMSVEPHNKPSNRISSSVSASPFIRQSKIRVPLLAKSFSDVNNSSVT FTEGDDNFNSKGSNDKSKLSMSSQY+K+NSFLLKYCSVDNIDKIDHSKVAKYQSWLSQPVFDEFFV
Sbjct: 1 MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQTNTSNQSNTSNVNVDGIGSTATISASSSTTSADSISASQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEK--------SDSQTTRGLTRFGIGSIKETDHESRNRNTLPSHSDSDSDTETTPTGDGVPASSNRTGVLNTIVEFGSNLFRQGGGGAQGSTSSDATRRNQQNKNKKSTSGSTSSMTGCTEGGAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCSIPAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVPTSGHTVSSSISSTPEKPAPKMMDMLFGSDTSTTIRSTSPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRSFISSTESSFVSKSNQNSSNPLIEHNIKSSNSMLSRSRPTSATTRSYNYSSHLDSTSNSQNENTDSQSHLPFDPIPETLFNSPLSNSKNCSHFSNVIPRKNLRYRSRSYIMSVEPHNKPSNRISSSVSASPFIRQSKIRVPLLAKSFSDVNNSSVTIFTEGDDNFNSKGSNDKSKLSMSSQYSKMNSFLLKYCSVDNIDKIDHSKVAKYQSWLSQPVFDEFFV 1125
BLAST of Rho-GAP domain-containing protein vs. Planmine SMEST
Match: SMESG000078332.1 (SMESG000078332.1) HSP 1 Score: 1657.11 bits (4290), Expect = 0.000e+0 Identity = 953/965 (98.76%), Postives = 953/965 (98.76%), Query Frame = 2
Query: 101 MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQIXXXXXXXXXXXXXDGIGXXXXXXXXXXXXXXXXXXXXQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEKHKFGHFKRSDSQTTRGLTRFGIGSIKETDHESRNRNTLPXXXXXXXXXXXXXXXXGVPASSNRTGVLNTIVEFGSNLFRXXXXXXXXXXXXXXXRRNQQNKNKKXXXXXXXXXXXXXXXXAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCSEPAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVPASGHTVXXXXXXTPEKPAPKMMDMLFGXXXXXXXXXXXPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRXXXXXXXXXXXXXXXXXXXXPLIEHNIKSSNSMLXXXXXXXXXXXXYNYSSHLDS 2995
MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQ NTSNQSNTSNVNVDGIGSTATISASSSTTSADSISASQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEK SDSQTTRGLTRFGIGSIKETDHESRNRNTLPSHSDSDSDTETTPTGDGVPASSNRTGVLNTIVEFGSNLFRQGGGGAQGSTSSDATRRNQQNKNKKSTSGSTSSMTGCTEGGAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCS PAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVP SGHTVSSSISSTPEKPAPKMMDMLFGSDTSTTIRSTSPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRSFISSTESSFVSKSNQNSSNPLIEHNIKSSNSMLSRSRPTSATTRSYNYSSHL S
Sbjct: 1 MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKLWCKFCKELVVAFEKKATLEIELCRSWSKQFQSFETTVSSSVMFGAPMRSESLSLSKLIIDQCKEIEIQNNKKLGKLLDVINRQRTEFSKTRKHYKEKWKSDCKKMMDAENTMLKTKSAYYQRCQQGVKLREELTNAQALLTYMQDHGIQETGSTNATAQTNTSNQSNTSNVNVDGIGSTATISASSSTTSADSISASQMSKQKLKVDKLEKQLADNDKKEIEQMYSYKESVENANNKLIEFEKKKMEHLCDIRLTTSRSDEVVKDTLAEYLRILAETRTTMLNEYSILSNKFQEYKPCYDYRCLIEKQLSNKPDIYSEKFQVELYHDCATTKEK--------SDSQTTRGLTRFGIGSIKETDHESRNRNTLPSHSDSDSDTETTPTGDGVPASSNRTGVLNTIVEFGSNLFRQGGGGAQGSTSSDATRRNQQNKNKKSTSGSTSSMTGCTEGGAEKDLEWQKEVDAVTEIVLKCIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQGADADLINEQPVTLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLFGPEDQYYNPKLTKGDLADFEETKIQQEKDSLNVLKVCSIPAVDSKVKQQRPHSSIIESAVNISALHESSFSRPLSGSLRVPTSGHTVSSSISSTPEKPAPKMMDMLFGSDTSTTIRSTSPRPKRITPEPSADKESISIDNNTKDKNSSSIPHRSISVGSGPLPEAIKLAQLSQPSNDSIFSKSKDNENITVTEKSVLNYTTGDRYNDLKRSFISSTESSFVSKSNQNSSNPLIEHNIKSSNSMLSRSRPTSATTRSYNYSSHLGS 957
BLAST of Rho-GAP domain-containing protein vs. Planmine SMEST
Match: SMESG000021141.1 (SMESG000021141.1) HSP 1 Score: 88.1965 bits (217), Expect = 2.315e-17 Identity = 55/184 (29.89%), Postives = 91/184 (49.46%), Query Frame = 2
Query: 1676 CIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQ---GADADLINEQPV------TLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
C IE GL G+YR S +M+ + D D+++E+ + L ++VKS + L P+ + LY+EF+ GK + + V K+ + LP N KL ++FHHLK I+ +SE+N M + NLG F+PT+ +P A + ++K ++ I+IE + F
Sbjct: 412 CFNSIERRGLENQGLYRTSGRVGYTDKIMKKIAEDLKCPIDGDVMSEKELENLEINVLCSMVKSYIKYLRAPMATFELYDEFISAGKLQNYEDKFNAVDKI---IKSLPKDNFKLFSILFHHLKIISESSEKNLMTAPNLGVCFSPTIF--RPEQVTASLTAIRESKFAGDVITILIEKCDDYF 590
BLAST of Rho-GAP domain-containing protein vs. Planmine SMEST
Match: SMESG000021141.1 (SMESG000021141.1) HSP 1 Score: 88.1965 bits (217), Expect = 2.398e-17 Identity = 55/184 (29.89%), Postives = 91/184 (49.46%), Query Frame = 2
Query: 1676 CIEVIEINGLSTHGIYRVSASKLKVGSVMESLNSQ---GADADLINEQPV------TLANVVKSKLISLSEPLVPYHLYNEFVDLGKEAESGEVSDLVTKLQLLVNQLPSSNRKLAGLIFHHLKKIANNSEENQMGSVNLGTLFAPTLIKSKPRFRMADIKEYMDNKSQAKIVQIIIENVETLF 2200
C IE GL G+YR S +M+ + D D+++E+ + L ++VKS + L P+ + LY+EF+ GK + + V K+ + LP N KL ++FHHLK I+ +SE+N M + NLG F+PT+ +P A + ++K ++ I+IE + F
Sbjct: 412 CFNSIERRGLENQGLYRTSGRVGYTDKIMKKIAEDLKCPIDGDVMSEKELENLEINVLCSMVKSYIKYLRAPMATFELYDEFISAGKLQNYEDKFNAVDKI---IKSLPKDNFKLFSILFHHLKIISESSEKNLMTAPNLGVCFSPTIF--RPEQVTASLTAIRESKFAGDVITILIEKCDDYF 590 The following BLAST results are available for this feature:
BLAST of Rho-GAP domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Rho-GAP domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009975 ID=SMED30009975|Name=Rho-GAP domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3875bpback to top protein sequence of SMED30009975-orf-1 >SMED30009975-orf-1 ID=SMED30009975-orf-1|Name=SMED30009975-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1134bp MSGDQDTENKLSADTITEASLDLSPGITEDDKLLWRKDKNAVDFSLNYAKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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