SMED30009969

Overview
NameSMED30009969
Smed IDSMED30009969
Length (bp)6024
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009969 (SMED30009969) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009969 (SMED30009969) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009969 (SMED30009969) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009969 (SMED30009969) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009969

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30009969 SmedASXL_077333SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30009969SMESG000064953.1 SMESG000050693.1 dd_Smed_v4_4882_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30009969SMESG000064953.1 SMESG000050693.1 dd_Smed_v4_4882_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
prepharyngeal regionSMED30009969 dd_Smed_v6_17573_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30009969 vs. Planmine SMEST
Match: SMESG000064895.1 (SMESG000064895.1)

HSP 1 Score: 272.707 bits (696), Expect = 1.465e-81
Identity = 172/282 (60.99%), Postives = 207/282 (73.40%), Query Frame = -3 
Query: 2708 NENTNSSMRIPRLPPSIVAIRHKYEDLKIQYRDLQTKHERVKGTWAPPEVVRELRQKISRLTQAREELIVESRAQRMLIREQSTALHQAYTETELRIKVPGSVRAEQIDIIPAHHLPNAVDQSNTVTNAXXXXXXXXXXXXSGATENPADSNAXXXXXXXXXXXXXXXXXXXXXXXXXXXQTTGGVEQLDDLPPPYQEFENSGMDPDIRYAPRILFSSPPASIHINLVDEEYEAHPLVQTLSDLELDFDEIAVLTGMVPNVGPRGEIAGPSSRGNEDPRGHP 3553
            NE+ N S RIPRLPPSIVA+RHKYEDLK Q+ DL+ K+ER+KGTWAPPE VRELRQKI R TQAR+ELI+ESR+Q++LIREQS AL QA+T+ ELR  V  SVRA Q+ IIPAHHLPN  D   +  +   S V      +    E   +S   TAR RPNRN+RRRDRRRQEE R R T+      ++DDLPPPY+EFE   +DPDI YAP+++FSSPP SIHINLVDEE E   +++ L  L LD  EIA+LTG VPNVGP+GEIAGPS++ N+D R  P
Sbjct:    5 NESNNLS-RIPRLPPSIVAVRHKYEDLKQQHNDLKAKYERMKGTWAPPETVRELRQKIFRFTQARDELIIESRSQQLLIREQSMALRQAFTDVELRTHVLESVRAGQLGIIPAHHLPNIEDLPASTVHISSSVVNPPSSLNPPLAEITTNSAISTARHRPNRNRRRRDRRRQEEERFRETRAAEDTYRIDDLPPPYREFEIPELDPDILYAPQVMFSSPPESIHINLVDEEIEPPRVIRALDRLGLDTGEIAILTGEVPNVGPQGEIAGPSTQSNDDYRVDP 285
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BLAST of SMED30009969 vs. Planmine SMEST
Match: SMESG000071419.1 (SMESG000071419.1)

HSP 1 Score: 201.83 bits (512), Expect = 3.914e-78
Identity = 125/291 (42.96%), Postives = 156/291 (53.61%), Query Frame = -1 
Query:  913 ENQKQTNRQVRRAQQRLDEINRDIATGLIPSTNGYAPPYNTSFNIDDPRLNVRIEAINVVVPR----RIWPERTRRTNEPSNTHGDHPYARGFPRRSSDQ*HLRTNSPRRFLVSSKNICFRLFFALWINNLNRSKSFES*NRSIDLRTHNKNNHHLLYMS*FHASIADMYSDQILQRELERLQKANSVLHQQVLQLTKEIQQTRATWVEPSRAKRIYQKMTVAQKGWVEEKQLNQNQKTQIRGLEVVLSACQEVAAVTYPLVFAPSQLAYREAMKVAKPATKPTRHRLGKS 1773
            E+Q+QT+RQV +A++ L+EINRDIA GLIPS  G   P  T+      R      A+NVVVPR    R W E     N+P+N H DHP+AR  P R  +                                  SK FE  N                          +M+    L+ +L++    N  LH+Q+ Q+  EIQ+ +ATW+EP +AK IYQKM  AQ+GW EEKQL Q+QK QIRGLEV LSACQE  AVTYPLVFAP+QLAYRE+    K ATKP RHR G+S
Sbjct:   87 EHQRQTDRQVDKARRHLEEINRDIAAGLIPSLTGM--PGRTTL-----RSTWMTSAMNVVVPREALIRGWGE----ANKPANEHHDHPHARERPSRRDN----------------------------------SKRFECLN--------------------------NMHPPLGLRDKLDKAHVKNRELHRQLTQVNSEIQRIKATWIEPGKAKLIYQKMMAAQRGWAEEKQLTQSQKVQIRGLEVALSACQEGKAVTYPLVFAPAQLAYRESTTATKIATKPPRHRPGRS 306

HSP 2 Score: 112.464 bits (280), Expect = 3.914e-78
Identity = 50/60 (83.33%), Postives = 54/60 (90.00%), Query Frame = -2 
Query: 1776 MSHFLENSWIDSSRVTLRFPAHSAARCRQAMWDVGFSISLVRESRVEDRTNADDTDLPFV 1955
            MSHFLENSWID+SRVTLRFPAHSA RCRQAMWD GFSIS VRESR+ +R+N DD DLPFV
Sbjct:    1 MSHFLENSWIDASRVTLRFPAHSAVRCRQAMWDAGFSISPVRESRIGNRSNTDDVDLPFV 60
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BLAST of SMED30009969 vs. Planmine SMEST
Match: SMESG000009535.1 (SMESG000009535.1)

HSP 1 Score: 250.751 bits (639), Expect = 7.058e-72
Identity = 158/297 (53.20%), Postives = 201/297 (67.68%), Query Frame = -3 
Query: 2660 RIPRLPPSIVAIRHKYEDLKIQYRDLQTKHERVKGTWAPPEVVRELRQKISRLTQAREELIVESRAQRMLIREQSTALHQAYTETELRIKVPGSVRAEQIDIIPAHHLPNA-------VDQSNTVTNAXXXXXXXXXXXXSGATENPADSNAXXXXXXXXXXXXXXXXXXXXXXXXXXXQTTGGVEQLDDLPPPYQEFENSGMDPDIRYAPRILFSSPPASIHINLVDEEYEAHPLVQTLSDLELDFDEIAVLTGMVPNVGPRGEIAGPSSRGNEDPRGHPPSYRPPKRKHLPTDIN 3529
            RIPRL PSIVA+RH+YE+L+ +Y  L  K+ERVKGTWA P+V++ LRQKI RLT+AR+EL+ ESR QR+LIREQSTAL +A+T+ ELR  VPG+VRA Q++IIPAHH+P         V   +T      ++ P      +GA E P  S     RRRPNRN+RRRDRRR+EE   +  +T+    Q  D PPPY+EFE   +DPDI YAP ++F SP  SIHINLVDE+    P+ Q L  L L  +EIA+L G VP +    E+AGPS+RG E+    PP YRP KRK+ PTDI+
Sbjct:   46 RIPRLSPSIVAVRHQYEELQRKYDALHAKYERVKGTWASPDVIKSLRQKIERLTRARDELVTESRIQRLLIREQSTALRRAFTDIELRTLVPGNVRASQLEIIPAHHIPVMGILPSLPVSNPSTSEARAPAATPVVSQPPTGAVETPTISTT---RRRPNRNRRRRDRRRREEELSQGVETSELSRQTGDPPPPYEEFEFPDLDPDICYAPNVVFRSPVPSIHINLVDEDPNPFPVTQVLDGLGLGTEEIAILMGEVPQIVSSQEVAGPSTRGEENRPTSPPPYRPMKRKYFPTDID 339
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BLAST of SMED30009969 vs. Planmine SMEST
Match: SMESG000075471.1 (SMESG000075471.1)

HSP 1 Score: 212.231 bits (539), Expect = 2.797e-58
Identity = 130/306 (42.48%), Postives = 179/306 (58.50%), Query Frame = -3 
Query: 2651 INENTNSSM----RIPRLPPSIVAIRHKYEDLKIQYRDLQTKHERVKGTWAPPEVVRELRQKISRLTQAREELIVESRAQRMLIREQSTALHQAYTETELRIKVPGSVRAEQIDIIPAHHLPNAVDQSNTVTNAXXXXXXXXXXXXSGATENPADSNAXXXXXXXXXXXXXXXXXXXXXXXXXXXQTTGGVEQLDDLPPPYQEFENSGMDPDIRYAPRILFSSPPASIHINLVDEEYEAHPLVQTLSDLELDFDEIAVLTGMVPNVGPRGEIAGPSSRGNEDPRGHPPSYRPPKRKHLPTDINAEE 3556
            +N  T SS+    RIPRL PS++A+R + E+L+ ++  L+TK+ER+KGTW+ PEV + L +K  RLT+AR+ELI ESR+QR+LI+EQ TAL QA+T+ ELR +VPGSV A Q++IIP HHLP  V+   T++    +     Q + +  + NP+ S A                                  Q    PP   EFE  G+DP+I YAP   F      IHINL+DE+ ++  + Q L  L L+ D+IAVLT  VP + P+ EIAGPS+R   +   +PP YRP KR+ LPTDI   E
Sbjct:    1 MNSGTGSSVNNLPRIPRLSPSVIALRQRLEELEAKHNSLRTKYERIKGTWSSPEVTQGLHRKKERLTRARDELIAESRSQRLLIKEQVTALRQAFTDEELRTQVPGSVGARQLNIIPEHHLP-IVEPPPTLS----ALFSLPQATVAETSVNPSQSKAT-------------------------------TSQGSGAPPGNGEFEIPGLDPNILYAPTATFPPLATQIHINLMDEDSDSPGVSQVLDRLGLNLDDIAVLTDSVPQISPQVEIAGPSTRIEGNHSVNPPPYRPAKRRCLPTDIRESE 270

HSP 2 Score: 98.9821 bits (245), Expect = 9.025e-21
Identity = 58/120 (48.33%), Postives = 72/120 (60.00%), Query Frame = -3 
Query: 2651 TTGGVEQLDDLPPPYQEFENSGMDPDIRYAPRILFSSPPASIHINLVDEEYEAHPLVQTLSDLELDFDEIAVLTGMVPNVGPRGEIAGPSSRGNEDPRGHPPSYRPPKRKHLPTDINAEE 3010
            +TG   + +DLPPPYQEFE    DPDI YAP ++  SP  SI+INLV++  EA  L   L  L L   EI VLTG VP   P     GPS+R   +   +PP  R PKR+ LP DI+AE+
Sbjct:  413 STGMPNRENDLPPPYQEFEFPNFDPDIIYAPSMINHSPARSIYINLVEDATEAPSLNHALEQLGLYIGEITVLTGRVPMSPPPANEPGPSTRVELNVITNPPLRRQPKRRCLPVDIDAED 532

HSP 3 Score: 59.6918 bits (143), Expect = 2.193e-8
Identity = 29/44 (65.91%), Postives = 33/44 (75.00%), Query Frame = -1 
Query: 2185 EGPSRSSARNGAHLLEWHSAIAYSTNMISVMG-TQWLLKSCDVS 2313
            EGPSRSS  N AHLLEWHSAIAYSTNMI VM  T+++    D +
Sbjct:  270 EGPSRSSVSNSAHLLEWHSAIAYSTNMILVMANTEYVRAHQDTT 313
[Close]
BLAST of SMED30009969 vs. Planmine SMEST
Match: SMESG000054981.1 (SMESG000054981.1)

HSP 1 Score: 191.045 bits (484), Expect = 6.822e-56
Identity = 91/129 (70.54%), Postives = 114/129 (88.37%), Query Frame = -3 
Query: 3176 MSINENTNSSMRIPRLPPSIVAIRHKYEDLKIQYRDLQTKHERVKGTWAPPEVVRELRQKISRLTQAREELIVESRAQRMLIREQSTALHQAYTETELRIKVPGSVRAEQIDIIPAHHLPNAVDQSNTV 3562
            M+ NE T++S RIPRLPPSIVA+RHKYEDLKIQ+RDLQTKH+RV+GTWAPPEV+RELRQKISRL++A +ELI ESRAQR+LIREQS ALHQAY+++ELRI+VPG++RAEQ+D++    L  A+  SN +
Sbjct:    1 MTGNEATSTSTRIPRLPPSIVALRHKYEDLKIQHRDLQTKHDRVRGTWAPPEVLRELRQKISRLSRALDELIAESRAQRLLIREQSVALHQAYSDSELRIRVPGNLRAEQLDVLRGVVLVMALTCSNGI 129
The following BLAST results are available for this feature:
BLAST of SMED30009969 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009969 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000064895.11.465e-8160.99SMESG000064895.1[more]
SMESG000071419.13.914e-7842.96SMESG000071419.1[more]
SMESG000009535.17.058e-7253.20SMESG000009535.1[more]
SMESG000075471.12.797e-5842.48SMESG000075471.1[more]
SMESG000054981.16.822e-5670.54SMESG000054981.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009969 ID=SMED30009969|Name=SMED30009969|organism=Schmidtea mediterranea sexual|type=transcript|length=6024bp
AATAATAATAAAAATAATAAATAATAATCGCGAAAAGATCTCTCCATAAC
GTCAATTAGGCGATTTTTATATCAATTTATCTATACTTATCCATATCTCT
GCAATATCAATGTTTAACCACTTGCGCTATGATTTAATTTTCGGTAGCTG
GGCCGCGCTCTACTATATATGTAAAGTATCGTAAGGCATCGACGTCTCAC
CGCGTCAGCGAGCTCAGGGGTTTGACCAGTGCTTAGCGCGAGGGTAGGGT
CTTACATAAAAGATTTACACGAATATAAATAATTATTCACAATAACAATA
AATTTTATTAATCAATAGTCTACTTTTGTATGATAATTTTCAAAACAAAT
TGGAATATGTAATGCCACAAGGCATTTCTTGCACTTCAAGTAGTCTCGCT
TCGTTTCTTATTATTGTTATTCATTTTATAATATGGGTGGGACTAAAATA
AAAAATATATTATTTTTTTAGCATTATAATAAAATAAAAACTGCTAACTG
CAAACCACAAGCATTTCCTTGTAAAAAATCAGTACAACATACCTTATCGA
TTCAGAAATATTCCTTATATCGATAACTCTACGCGATGTGAACTAACTTT
ATAATTGCAGTTCGAATAATGACGTTACGTCTGTCAGCCGACGTTGCACA
CACGTCGTTTTTGTTTTTGCGACAAATGCTACCGACGTGGTATACACGTC
ACTTGCGGTTTTGAAAAAAATCATCAAGGCGTGCCTGGCACGTCAATATA
GCGCAAGTGGTTAAATCTAATATCATCTAACTTATCGTTGGTGGACTTTC
GGTTTTAATGAAATTATCTTTAATAACATAAGATATAGGGGAAGTCGATT
TTAAAATCTTTTATTAAACGACAGACGGATCATTATTGTGTCATTCCTTA
TTCAATTTTTCCGGATTTTCCGAGTCGGTGTCGAGTTGGTTTGGTGGCCG
GTTTTGCGACTTTCATTGCCTCTCGATAGGCTAATTGCGACGGGGCGAAA
ACTAACGGGTAGGTTACCGCAGCGACCTCTTGGCACGCCGACAACACTAC
CTCCAGACCTCTGATTTGGGTCTTCTGATTTTGGTTGAGCTGTTTTTCTT
CAACCCAACCCTTTTGCGCCACCGTCATCTTTTGGTAGATGCGTTTTGCT
CTACTCGGCTCCACCCATGTGGCCCTAGTCTGTTGTATCTCCTTAGTCAA
CTGTAAGACTTGTTGGTGGAGCACGGAATTAGCTTTCTGCAGTCGTTCTA
ACTCTCGTTGTAGGATCTGGTCGGAGTACATATCTGCAATAGAAGCGTGA
AATCAACTCATATATAATAAATGGTGGTTATTTTTATTATGGGTCCTTAG
ATCTATGGAACGGTTTTATGACTCGAATGATTTACTTCTATTTAAATTAT
TAATCCACAAGGCAAAGAATAACCTAAAACATATATTTTTACTAGACACT
AGAAAACGTCGAGGTGAGTTAGTTCTCAGATGTTATTGATCCGAGCTCCT
TCGCGGGAACCCACGAGCATATGGATGATCGCCATGAGTGTTGGACGGTT
CGTTTGTTCTCCTTGTTCTTTCTGGCCAAATCCGGCGGGGGACCACCACG
TTGATTGCTTCTATTCGCACATTTAGCCTGGGATCGTCTATGTTGAAGGA
CGTGTTGTATGGAGGGGCATACCCATTTGTACTTGGGATCAGTCCAGTCG
CGATATCCCGGTTGATTTCGTCTAAACGTTGCTGTGCTCTCCGGACTTGG
CGGTTAGTTTGCTTCTGGTTCTCGCGACGAATGGGAGGTCCGTGTCGTCT
GCATTTGTTCGATCTTCCACCCGGGACTCTCTGACCAGCGAGATTGAAAA
TCCCACGTCCCACATCGCTTGCCGACACCGAGCTGCTGAGTGTGCCGGAA
AGCGTAATGTGACCCGGGACGAGTCGATCCAAGAATTTTCTAAAAAATGA
GACATTTATTAACATAACTCGTTTATCCCGTCACTAGCACTGAAGTCGAT
GGGGCTGTGCCCTGATAATGGTTCGTCGTGCTCGGGTATGAAAATTTGCT
AACCCGGTCTTGGTTAGTGCTAAGGACGTGGGTATTGAATTTATCTTGCT
GTTGGACGTACAGGAGTTTTAAACTAAGGTACTATTCATCTCACTGATCT
GACCTTCACTTGCAGGTTCTAGGGATAATTTACCTGATACGTCGCACGAT
TTTAATAACCATTGCGTACCCATGACCGAAATCATGTTGGTACTGTACGC
AATAGCCGAATGCCATTCGAGCAGGTGAGCGCCATTTCTAGCACTACTCC
TCGAAGGACCTTCGTCTTTCCCGGATTATAGCTGCCGATAGAAGGACTTA
CGCTCTCCCTCAAAACGTAGCAAAAACCCTCTATTCCTTGACATTACGTT
CACTTCAGAAGTGGGAATTATGTAACAACCTCGTTCTCGTAACCCCGCGC
GTCCGTAATTCTCATCTCCGATTGTTCCTACTTTCGATTTATAAAGAGAA
ACATAAATGTAGCAATATTTATTAGGAAACAAAACAACATTTAATCAAGA
GCAAAAATAATAAACGGAAATGAAAATAAACAGTTCGAGCACTGGTTGAA
GTAAAAACCACTCATAGTGTGAGTAAACTCGGGATTTCTGGCGGTTAGCT
TTCTTCCGCGTTTATATCCGTCGGGAGGTGTTTCCGTTTAGGTGGTCGGT
ACGATGGAGGATGGCCTCGAGGATCCTCGTTTCCCCGCGATGATGGTCCA
GCGATTTCGCCACGGGGACCAACGTTGGGGACCATTCCCGTGAGAACAGC
GATCTCATCGAAGTCCAACTCAAGGTCACTTAGCGTCTGCACCAACGGGT
GAGCTTCATATTCCTCATCCACCAGGTTAATATGTATGGAAGCTGGGGGA
GAGGAGAATAAGATTCGCGGGGCATATCGGATATCCGGATCCATCCCCGA
GTTCTCGAACTCTTGGTAAGGGGGTGGGAGATCATCCAGTTGTTCCACCC
CTCCTGTCGTCTGAGTTCTCCGGAGGCGGGCCTCTTCTTGCCTGCGACGA
TCTCGACGTCGCTTGTTCCGGTTTGGCCGACGACGGGCCGTAGGGGCGTT
GGAGTCGGCCGGGTTTTCGGTAGCTCCGGAAGATGATGGTTGAGAGGTGG
GCACACTCGAGATAGGAGCATTAGTTACTGTATTAGATTGGTCCACGGCA
TTGGGGAGGTGGTGAGCAGGGATGATGTCGATCTGCTCAGCCCGAACACT
CCCTGGGACCTTGATCCGGAGCTCCGTTTCGGTATAGGCCTGGTGTAGGG
CCGTTGATTGTTCGCGGATCAACATCCGCTGGGCGCGCGACTCGACGATG
AGTTCCTCGCGCGCTTGCGTAAGTCGTGAGATCTTCTGCCTGAGCTCCCT
GACGACTTCTGGTGGAGCCCAGGTCCCTTTTACTCTTTCGTGCTTCGTTT
GAAGATCCCTATATTGGATTTTAAGATCTTCGTATTTGTGGCGGATCGCC
ACGATTGATGGAGGCAGGCGAGGAATCCTCATTGAAGAGTTAGTGTTCTC
GTTGATTGACATACTATTGGAAAGGAGGTAATATATTTAATAGCCAAACA
AGAAAAATTAATAATAAAAAAAAAATAGAAAAAAATTCGAAAGAGGAAAT
TTTAAAGGTAATTCGCGCGACGTCGAAAATCGGAGCATCGGTGATGATTA
GGACGGCTTTCTCGGGTGTACCCTCATTCCCTTCATAAGTGTGATTTATT
TGACAAGCTAGTCGTTATTAATTTCGGAAATATCATTACCAAAACATTTA
CTCAGCAAGTTACACATTCGAGATCATTATTGTTCTAAAACCTAAACTCC
TACTCGAACCGATATTTGGTAGGGGAGTTAATGGGTAGTTTGCCGACTTG
ATATCTAGCCTCACGTTCTGAGTTACTATAGGTGTCATGGGTACGATTAA
TAATTTAGCGTCTATCTCCCCCATTTTCTTCGAACCGCTCTACAGTCATG
GATAGATAGATTTTAATAGCAACAACAAAAAAAAATTTAATTTTTATTTG
ATTTTACAACGCCACTAGAATTTTTATTGATTATTTAAGCGATTTGATGC
CGCCTACCGCAACTACATACCGGTAGGTGCGATCAAATAATTTCTGAGCT
ATGATGCGCTGATCCATGAACGGTTTATTATGTCTTCACATTTTTGGGTC
TCTCGTCTTAACCTGTGGTTTCAAATCACTTACTCTACTGATTGCGGATT
TATTAAACATTATTCATGTCGTCAACTACCTCTAGTCTTAGTGGATATAT
AATTTTCCAAGAAAATGGTTTGTCCAGCACCGTTCCGATCACATGACATT
CTCTTACTCAGGTTCTCAGATGGAATAAGATAATTAGAGAGTGGTTACAC
GTGCGATTCTCCCGTGGAACATTTTTGCAAGGTAATAAATTTTCCGTTAT
CATCTAAATTTGTCTGAATATTTGGTAATTTTGCGATTCGCTTGCTTTAA
AACTTAAAAGTTGTCTTTCTTAAGTCTTATCCGTACTACCCAATTCTACT
TTTTGTTCGGAATATTAAAAATCTGCTCCAGATCTATATGTGATCCGCCC
TGATTCTCCTAACTACAGTTCTCCGCACTTCCCGAAAATTTTTAGCTATT
CGCTCCGGCCACATTTTCAGCAGCCGCCTCGAATATACAATGGCGCCCTG
CGTAGCGGTCCCGTGCACAAAGCCGGAACCGACTGCGAACTGCCAAGGGG
TTCACGAGGCATGACCCACGAGCTCAACTAAGGGTTTTCACCCACTAGTT
AGCCCGAGATCCGTCCCATGGACCATCTATTGAAATACTTATTATAAATA
AGATTTTAGGGGGATTTGTCAGTGGCACCAAAAGATTCCACGACCATCCA
GAATAAAATCAAAGTAACAAGACTTCGAAATATATATTTATACAAGAAAA
CAATACTTACAATCCACAGTCATGCAACCAGATTTGAAAAATTGGCATAA
ATTGGCATACAAAATTTGGTCTAAGAATGATTCACTGCTAGTCTAAAAAT
TTCTACAAATTTTCTAAAAATTTTCTGCCGGACTAAAATTTTCTAAGTCT
GTTAAAATTCGGTTAGAAATTATGAAAATGACTGCCGTATATATAGTATT
TTTGTGCCTGTGTGCGACCAATGGGCGATTTTTCGCCATTTTTAGGGCTT
TTTAATTATTTTATTTATTTCAAAATATTTTTGTAATTTTTTATATATTT
TTCTTTATTTTTTGATATATTAATATTTTATTGTTGGATTTTATTTTTAT
TATTATTTTCTTAAAATATTATTTTTAAACCCCAATATTTTGTTTTCTGG
TCGCCTCTTCTGCCTGTGTTTTTTCTAAACCTTATTTTGCAAGGTTATAG
GTGGTTTTGTGTTTTACTTTATTTTTTCATATGCAACTGTGTGCGAAATT
TATTAATTATGTTATTTTTTTATTTTGTTTAATTTGGTGTTTTTTTATTT
AGTTTTGTTTTCTGTTTGCGGTGTATGCTTATGTTATAGCAGCCATTTTG
ACAATTTCCAAACCTCATTAATTATTTTGTTTTAATTTACACAATTTTCT
CATTTTTGTTTAAATTATGGCTTATAGCCCAACACTATTAATTACACCAA
AGCGCATTAAATTGGAGCTAATGAGACAGCATTATGGATTTTATATAATC
AATCAAAAATCTAACCAATAATAAATGTGTGTGTCATAGCTAAAATAGAA
TATTGGTAAGAGCTGCAGCATTAGGTAGGGGATTATATTATAGAATGGCA
GAGAAATTTCATAAGAAAAAATTACCTGCACACAATCTTGGTTCAGAAAA
TTTCTATAATTAAAATGCTAGTAAATTAGTCAATAAATTGCACCTTGGTA
AATAAATTGTCGCTATTTTTATACTTTATTTTAAAAATACTTTATTTTTT
CAAAGATATTTCCAATGTTTCATCGGCATCAACAGTTGCGTACAAGCAAA
AGAACAAAAATATGTTATATCTAT
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protein sequence of SMED30009969-orf-1

>SMED30009969-orf-1 ID=SMED30009969-orf-1|Name=SMED30009969-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=128bp
MYSDQILQRELERLQKANSVLHQQVLQLTKEIQQTRATWVEPSRAKRIYQ
KMTVAQKGWVEEKQLNQNQKTQIRGLEVVLSACQEVAAVTYPLVFAPSQL
AYREAMKVAKPATKPTRHRLGKSGKIE*
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protein sequence of SMED30009969-orf-2

>SMED30009969-orf-2 ID=SMED30009969-orf-2|Name=SMED30009969-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=306bp
MSINENTNSSMRIPRLPPSIVAIRHKYEDLKIQYRDLQTKHERVKGTWAP
PEVVRELRQKISRLTQAREELIVESRAQRMLIREQSTALHQAYTETELRI
KVPGSVRAEQIDIIPAHHLPNAVDQSNTVTNAPISSVPTSQPSSSGATEN
PADSNAPTARRRPNRNKRRRDRRRQEEARLRRTQTTGGVEQLDDLPPPYQ
EFENSGMDPDIRYAPRILFSSPPASIHINLVDEEYEAHPLVQTLSDLELD
FDEIAVLTGMVPNVGPRGEIAGPSSRGNEDPRGHPPSYRPPKRKHLPTDI
NAEES*
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protein sequence of SMED30009969-orf-3

>SMED30009969-orf-3 ID=SMED30009969-orf-3|Name=SMED30009969-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp
MLINVSFFRKFLDRLVPGHITLSGTLSSSVSASDVGRGIFNLAGQRVPGG
RSNKCRRHGPPIRRENQKQTNRQVRRAQQRLDEINRDIATGLIPSTNGYA
PPYNTSFNIDDPRLNVRIEAINVVVPRRIWPERTRRTNEPSNTHGDHPYA
RGFPRRSSDQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000419prepharyngeal region