U4/U6.U5 tri-snRNP-associated protein 1

Overview
NameU4/U6.U5 tri-snRNP-associated protein 1
Smed IDSMED30009928
Length (bp)2136
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of U4/U6.U5 tri-snRNP-associated protein 1 (SMED30009928) t-SNE clustered cells

Violin plots show distribution of expression levels for U4/U6.U5 tri-snRNP-associated protein 1 (SMED30009928) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of U4/U6.U5 tri-snRNP-associated protein 1 (SMED30009928) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for U4/U6.U5 tri-snRNP-associated protein 1 (SMED30009928) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009928

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30009928SMESG000047653.1 SmedASXL_012100SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30009928SMESG000047653.1 dd_Smed_v4_5108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009928SMESG000047653.1 dd_Smed_v4_5108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009928SMESG000016758.1 Contig4948GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009928SMESG000047653.1 Contig4948GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Human
Match: SART1 (spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP [Source:HGNC Symbol;Acc:HGNC:10538])

HSP 1 Score: 102.834 bits (255), Expect = 5.548e-22
Identity = 64/172 (37.21%), Postives = 95/172 (55.23%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +N G+ + LLL +NKG LE    +   +  P   +  A Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  627 ILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDD---KYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNA 795

HSP 2 Score: 97.8265 bits (242), Expect = 2.263e-20
Identity = 101/298 (33.89%), Postives = 144/298 (48.32%), Query Frame = 3 
Query:   72 GDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIEN---------ESSDEEFSDKPMYKRDELFGISVEHKASRFDEAGITI--------------LTLKDKNILDEEKDDVLVNINILDREREEKNLR-NKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            GD SSLSIEETNKLR +LGLKPLE N +  E  T+    +  V    IN         L  + ++  +    KEK L  + LG    L  D+         +E   ++QK ++         E  D+EF    +   +E FG   +   S  D  G+T+              LTLKDK +L EE +DVLVN+N++D+ER EKN+   KK   Y  + +    DD L   KP S+LSKYDE ++G + + F L+  G  +  +ER  +  R       +      P +AS+YLTP E+
Sbjct:  113 GDASSLSIEETNKLRAKLGLKPLEVNAIKKEAGTK----EEPVTADVIN------PMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLV--EEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKGVLQEE-EDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDD-LAQQKPRSILSKYDEELEGERPHSFRLEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEM 396
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Celegans
Match: F19F10.9 (pep chromosome:WBcel235:V:7571950:7575715:-1 gene:WBGene00017605.1 transcript:F19F10.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F19F10.9)

HSP 1 Score: 76.2554 bits (186), Expect = 5.383e-14
Identity = 52/171 (30.41%), Postives = 85/171 (49.71%), Query Frame = 3 
Query: 1542 LGDEVQINSGIYSTLLLVKNKGYLED----KKSQPLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNL----PEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKLIKMHSKMKSENRFCVP---LSG 2021
            LG EV ++ G+   L L   KGYL++      S P + + KNK  +    Q    R+D  D+   + DR N     P MPF +K+ + P++ + Y D +GR    K+A+R LS++FHG+  GK +  KR  +  ++ ++      D  L  +  + K + +   P   LSG
Sbjct:  653 LGQEVDVSKGVGRMLKLAGQKGYLQNPNAKSHSGPSLDHLKNKTTQRIDGQ----RFDPDDRNAKKADRLNSQHRGPVMPFAEKHDYKPDVNISYVDRKGREMDAKDAYRELSYKFHGRNPGKKQLEKRANRKDKEERMLKTNSYDTPLGTLDKQRKKQKQLSTPYLVLSG 819

HSP 2 Score: 63.5438 bits (153), Expect = 4.025e-10
Identity = 53/147 (36.05%), Postives = 83/147 (56.46%), Query Frame = 3 
Query:  468 GISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLR-NKKMGAYGAFVDSGDED-DPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTE-DTYRKSFFSGTK-HSINTEPI-IASDYLTPLEI 893
            G+ + H    F E G  IL L+DK +LD+  D+VLVN N+LD ER  +N+   K+      F    DED D     K   VL+KYDE ++G ++ +F LD  G  ++E+ER E + +R+   +G +  S+ T+   +AS++ T  E+
Sbjct:  201 GMVIGHGREAFIE-GDQILVLQDKGVLDD-GDEVLVNPNLLDNERHVRNVELRKRKDPNRNF----DEDVDEFGNAKNFGVLAKYDETLEGEQKKQFRLDEHGGVDLEEERREMEAFRRMKMAGKRLESLETKKYELASEFYTQDEM 341
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Fly
Match: CG6686 (gene:FBgn0032388 transcript:FBtr0089573)

HSP 1 Score: 106.301 bits (264), Expect = 2.786e-23
Identity = 75/175 (42.86%), Postives = 98/175 (56.00%), Query Frame = 3 
Query: 1536 GILGDEVQINSGIYSTLLLVKNKGYLE-DKKSQPLISNPKNKMLRAQ-YFQEEKIRYDDIDQKCPRRDRRNL-PEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQ---KLIKMHSKMKSENRFCVPLSGKNSIIPA 2042
             IL +E  + +G+ + L L  +KGYLE ++K++P  SN K   L+A+ Y  E+K   +D  +K  RRDR +  P   F DK TF P +KL+Y DD GR    KEAFR LSH+FHGK  GK K  KRLKKM +   + TM  TD     L  +  K K      V LSG N  +P 
Sbjct:  784 AILDEEPDVGAGVANALRLALSKGYLEKEEKNRP--SNTKMAHLQAKNYSIEDKAAGED--EKVGRRDRFHFGPITDFKDKETFKPNVKLDYIDDNGRILNLKEAFRYLSHKFHGKGPGKNKIEKRLKKMEQDGLMKTMSSTDTPLGTLTMLQQKQKETKTAYVVLSGNNKNVPG 954

HSP 2 Score: 70.4774 bits (171), Expect = 3.815e-12
Identity = 48/123 (39.02%), Postives = 70/123 (56.91%), Query Frame = 3 
Query:  447 YKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMG----AYGAFVDSGDE-DDPLTLLKPPSVLSKYDEVIDGV--KRNRFVLDSKGKYNMEQER 794
            Y    L G+ V+H    F E    ILTLKD+++L+EE+ D LVN+N++D ER +KN+ NKK       Y  + +  DE  +P+      SVL KYD+ ++G   KR  FV+      NM++ER
Sbjct:  429 YGDSNLKGLRVDHDMDDFGEGRTVILTLKDQDVLNEEEGDTLVNVNMVDEERYKKNVANKKQNPLSYGYNVYEEQYDELGNPIER----SVLEKYDDEMEGQPKKRKNFVIGE----NMDEER 543
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Fly
Match: CG6686 (gene:FBgn0032388 transcript:FBtr0089572)

HSP 1 Score: 106.301 bits (264), Expect = 2.800e-23
Identity = 75/175 (42.86%), Postives = 98/175 (56.00%), Query Frame = 3 
Query: 1536 GILGDEVQINSGIYSTLLLVKNKGYLE-DKKSQPLISNPKNKMLRAQ-YFQEEKIRYDDIDQKCPRRDRRNL-PEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQ---KLIKMHSKMKSENRFCVPLSGKNSIIPA 2042
             IL +E  + +G+ + L L  +KGYLE ++K++P  SN K   L+A+ Y  E+K   +D  +K  RRDR +  P   F DK TF P +KL+Y DD GR    KEAFR LSH+FHGK  GK K  KRLKKM +   + TM  TD     L  +  K K      V LSG N  +P 
Sbjct:  790 AILDEEPDVGAGVANALRLALSKGYLEKEEKNRP--SNTKMAHLQAKNYSIEDKAAGED--EKVGRRDRFHFGPITDFKDKETFKPNVKLDYIDDNGRILNLKEAFRYLSHKFHGKGPGKNKIEKRLKKMEQDGLMKTMSSTDTPLGTLTMLQQKQKETKTAYVVLSGNNKNVPG 960

HSP 2 Score: 70.4774 bits (171), Expect = 3.825e-12
Identity = 48/123 (39.02%), Postives = 70/123 (56.91%), Query Frame = 3 
Query:  447 YKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMG----AYGAFVDSGDE-DDPLTLLKPPSVLSKYDEVIDGV--KRNRFVLDSKGKYNMEQER 794
            Y    L G+ V+H    F E    ILTLKD+++L+EE+ D LVN+N++D ER +KN+ NKK       Y  + +  DE  +P+      SVL KYD+ ++G   KR  FV+      NM++ER
Sbjct:  435 YGDSNLKGLRVDHDMDDFGEGRTVILTLKDQDVLNEEEGDTLVNVNMVDEERYKKNVANKKQNPLSYGYNVYEEQYDELGNPIER----SVLEKYDDEMEGQPKKRKNFVIGE----NMDEER 549
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Zebrafish
Match: sart1 (spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP [Source:ZFIN;Acc:ZDB-GENE-040718-422])

HSP 1 Score: 107.842 bits (268), Expect = 9.422e-24
Identity = 68/173 (39.31%), Postives = 98/173 (56.65%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQPL--ISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +NSG+ + LLL KNKG L D + Q +  +  PK  +    Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K KS+    + LSG    + A
Sbjct:  604 ILDEEPIVNSGLAAALLLCKNKGLL-DTQMQKVARVKAPKPVLANDNYCIEDKMHIDD---KYSRREEYRGFTQDFKEKDNYKPDVKIEYVDESGRKLCPKEAFRQLSHRFHGKGSGKMKTERRMKKLEEEALLKKMSSSDTPLGTVALLQEKQKSQKTPYIVLSGSGKSMNA 772

HSP 2 Score: 93.5893 bits (231), Expect = 3.091e-19
Identity = 58/160 (36.25%), Postives = 90/160 (56.25%), Query Frame = 3 
Query:  420 DEEFSD--KPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            ++EF+   +  Y   +L G+ V+HK   F E    +LTL+DK +L+EE +DVLVN+ ++D+E+ EKN+  KK        +  +  D + + KP SVLSKYDE I+G K+  F L S G    E+ER     R+S     +    +   +A++Y TP E+
Sbjct:  218 EQEFAQEKRQAYSSHDLKGLKVQHKVESFSEGQTVVLTLQDKGVLEEE-EDVLVNVGLIDKEKAEKNVELKKKKPDYKPYEEEESVDDMIVFKPKSVLSKYDEEIEGEKKKSFRLSSGGFVGGERERELQAIRESLRDQAQSLSTSALTLATEYYTPQEM 376
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000022048.1 (SART1, U4/U6.U5 tri-snRNP-associated protein 1 [Source:NCBI gene;Acc:100485596])

HSP 1 Score: 118.242 bits (295), Expect = 7.458e-27
Identity = 106/295 (35.93%), Postives = 153/295 (51.86%), Query Frame = 3 
Query:   72 GDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIENESSD--------------------EEFSDKPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINT-EPIIASDYLTPLEI 893
            GD SSLSIEETNKLR +LGLKPLE N V  E  T+    ++ V    IN         L+ + ++  +    KEK L  + LG   +L +DE         +E   K+Q +E E ++                    EEF  K  Y   +L G++VEHK + F E    ILTLKDK +LDE+  DVLVN+N++D+E+ EKN+  KK        +  +  + + + +P SVLSKYDE I+G K+  F L+S G  +   E+     R+S  S   H++   +  +AS+Y TP E+
Sbjct:   89 GD-SSLSIEETNKLRAKLGLKPLEVNTVKTESGTK----EDPVAASVIN------PIALKQQQELREKLAAAKEKRLLNQKLGKIKSLGDDELWLDDTAAWIEKSRKLQ-MEKEIAEKRAKLLAEMDEEFGVSNLVEEEFGQKKHYSSRDLQGLTVEHKINAFKEGQTVILTLKDKGVLDEDDADVLVNVNLVDKEKAEKNVELKKKKPEYKPYEEEENVEDMAVFRPKSVLSKYDEEIEGEKKKSFQLESGGVADGSWEKELQNIRESLHSQA-HTLELPQSKLASEYYTPEEM 370

HSP 2 Score: 105.916 bits (263), Expect = 5.658e-23
Identity = 65/172 (37.79%), Postives = 94/172 (54.65%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +N G+ + LLL KNKG LE    +   +  P   +  A Y  E+K+  DD   K  RR+        F +K  + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  593 ILDEEPIVNRGLGAALLLCKNKGLLETTVQKVARVKTPNKSLPSAVYCIEDKMAIDD---KYSRREEYRGFTQDFKEKEGYRPDVKIEYVDETGRKLCPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMTA 761
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000022057.1 (SART1, U4/U6.U5 tri-snRNP-associated protein 1 [Source:NCBI gene;Acc:100485596])

HSP 1 Score: 118.242 bits (295), Expect = 7.722e-27
Identity = 106/295 (35.93%), Postives = 153/295 (51.86%), Query Frame = 3 
Query:   72 GDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIENESSD--------------------EEFSDKPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINT-EPIIASDYLTPLEI 893
            GD SSLSIEETNKLR +LGLKPLE N V  E  T+    ++ V    IN         L+ + ++  +    KEK L  + LG   +L +DE         +E   K+Q +E E ++                    EEF  K  Y   +L G++VEHK + F E    ILTLKDK +LDE+  DVLVN+N++D+E+ EKN+  KK        +  +  + + + +P SVLSKYDE I+G K+  F L+S G  +   E+     R+S  S   H++   +  +AS+Y TP E+
Sbjct:  125 GD-SSLSIEETNKLRAKLGLKPLEVNTVKTESGTK----EDPVAASVIN------PIALKQQQELREKLAAAKEKRLLNQKLGKIKSLGDDELWLDDTAAWIEKSRKLQ-MEKEIAEKRAKLLAEMDEEFGVSNLVEEEFGQKKHYSSRDLQGLTVEHKINAFKEGQTVILTLKDKGVLDEDDADVLVNVNLVDKEKAEKNVELKKKKPEYKPYEEEENVEDMAVFRPKSVLSKYDEEIEGEKKKSFQLESGGVADGSWEKELQNIRESLHS-QAHTLELPQSKLASEYYTPEEM 406

HSP 2 Score: 105.531 bits (262), Expect = 8.623e-23
Identity = 65/172 (37.79%), Postives = 94/172 (54.65%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +N G+ + LLL KNKG LE    +   +  P   +  A Y  E+K+  DD   K  RR+        F +K  + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  629 ILDEEPIVNRGLGAALLLCKNKGLLETTVQKVARVKTPNKSLPSAVYCIEDKMAIDD---KYSRREEYRGFTQDFKEKEGYRPDVKIEYVDETGRKLCPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMTA 797
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Xenopus
Match: ENSXETT00000022014.1 (SART1, U4/U6.U5 tri-snRNP-associated protein 1 [Source:NCBI gene;Acc:100485596])

HSP 1 Score: 105.531 bits (262), Expect = 5.169e-23
Identity = 65/172 (37.79%), Postives = 94/172 (54.65%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +N G+ + LLL KNKG LE    +   +  P   +  A Y  E+K+  DD   K  RR+        F +K  + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  450 ILDEEPIVNRGLGAALLLCKNKGLLETTVQKVARVKTPNKSLPSAVYCIEDKMAIDD---KYSRREEYRGFTQDFKEKEGYRPDVKIEYVDETGRKLCPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMTA 618

HSP 2 Score: 98.9821 bits (245), Expect = 6.251e-21
Identity = 95/287 (33.10%), Postives = 144/287 (50.17%), Query Frame = 3 
Query:  105 NKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIENESSD--------------------EEFSDKPM---YKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINT-EPIIASDYLTPLEI 893
            +KLR +LGLKPLE N V  E  T+    ++ V    IN         L+ + ++  +    KEK L  + LG   +L +DE         +E   K+Q +E E ++                    EEF  K +   Y   +L G++VEHK + F E    ILTLKDK +LDE+  DVLVN+N++D+E+ EKN+  KK        +  +  + + + +P SVLSKYDE I+G K+  F L+S G  +   E+     R+S  S   H++   +  +AS+Y TP E+
Sbjct:   10 SKLRAKLGLKPLEVNTVKTESGTK----EDPVAASVIN------PIALKQQQELREKLAAAKEKRLLNQKLGKIKSLGDDELWLDDTAAWIEKSRKLQ-MEKEIAEKRAKLLAEMDEEFGVSNLVEEEFGQKKVGDHYSSRDLQGLTVEHKINAFKEGQTVILTLKDKGVLDEDDADVLVNVNLVDKEKAEKNVELKKKKPEYKPYEEEENVEDMAVFRPKSVLSKYDEEIEGEKKKSFQLESGGVADGSWEKELQNIRESLHSQA-HTLELPQSKLASEYYTPEEM 284
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Mouse
Match: Sart1 (squamous cell carcinoma antigen recognized by T cells 1 [Source:MGI Symbol;Acc:MGI:1309453])

HSP 1 Score: 102.064 bits (253), Expect = 6.934e-22
Identity = 64/172 (37.21%), Postives = 95/172 (55.23%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +N G+ + LLL +NKG LE    +   +  P   +  A Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  633 ILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDD---KYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNA 801

HSP 2 Score: 87.0409 bits (214), Expect = 4.116e-17
Identity = 57/161 (35.40%), Postives = 92/161 (57.14%), Query Frame = 3 
Query:  420 DEEFSDK--PMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLR-NKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            +EEF  +   +Y   +L G++VEH    F E    +LTLKDK +L ++ +DVLVN+N++D+ER +KN+   KK   Y  + +    DD L   KP S+L+KYDE ++G + + F L+  G  +  +ER  +  R       +   +  P +AS+YL+P E+
Sbjct:  238 EEEFEQRRQDLYSARDLQGLTVEHAIDSFREGETVVLTLKDKGVL-QDGEDVLVNVNMVDKERADKNVELRKKKPDYLPYAEDESVDD-LAQQKPRSILAKYDEELEGERPHSFRLEQGGMADGLRERELEEIRTKLRLQAQSLSSVGPRLASEYLSPEEM 396
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Mouse
Match: Sart1 (squamous cell carcinoma antigen recognized by T cells 1 [Source:MGI Symbol;Acc:MGI:1309453])

HSP 1 Score: 50.8322 bits (120), Expect = 2.350e-7
Identity = 25/35 (71.43%), Postives = 27/35 (77.14%), Query Frame = 3 
Query:   72 GDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTE 176
            GD SSLSIEETNKLR +LGLKPLE N V  E  T+
Sbjct:   38 GDASSLSIEETNKLRAKLGLKPLEVNAVKKEAGTK 72
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. UniProt/SwissProt
Match: sp|O43290|SNUT1_HUMAN (U4/U6.U5 tri-snRNP-associated protein 1 OS=Homo sapiens OX=9606 GN=SART1 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.664e-21
Identity = 64/172 (37.21%), Postives = 95/172 (55.23%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +N G+ + LLL +NKG LE    +   +  P   +  A Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  627 ILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDD---KYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNA 795

HSP 2 Score: 95.1301 bits (235), Expect = 7.710e-19
Identity = 63/161 (39.13%), Postives = 91/161 (56.52%), Query Frame = 3 
Query:  420 DEEFSDK--PMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLR-NKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            +EEF  +   +Y   +L G++VEH    F E    ILTLKDK +L EE +DVLVN+N++D+ER EKN+   KK   Y  + +    DD L   KP S+LSKYDE ++G + + F L+  G  +  +ER  +  R       +      P +AS+YLTP E+
Sbjct:  238 EEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKGVLQEE-EDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDD-LAQQKPRSILSKYDEELEGERPHSFRLEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEM 396
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. UniProt/SwissProt
Match: sp|Q5XIW8|SNUT1_RAT (U4/U6.U5 tri-snRNP-associated protein 1 OS=Rattus norvegicus OX=10116 GN=Sart1 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 4.221e-21
Identity = 64/172 (37.21%), Postives = 95/172 (55.23%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +N G+ + LLL +NKG LE    +   +  P   +  A Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  633 ILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDD---KYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNA 801

HSP 2 Score: 88.1965 bits (217), Expect = 1.085e-16
Identity = 61/162 (37.65%), Postives = 95/162 (58.64%), Query Frame = 3 
Query:  420 DEEFSDK--PMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLR-NKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINT-EPIIASDYLTPLEI 893
            +EEF  +   +Y   +L G++VEH    F E    +LTLKDK +L +E +DVLVN+N++D+ER +KN+   KK   Y  + +    DD L   KP S+L+KYDE ++G + + F L+  G  +  +ER  +  R +       S+NT  P +AS+YL+P E+
Sbjct:  238 EEEFEQRRQDLYSARDLQGLTVEHAIDSFREGETVVLTLKDKGVL-QEGEDVLVNVNMVDKERADKNVELRKKKPDYLPYAEDESVDD-LAQQKPRSILAKYDEELEGERPHSFRLEQGGMADGLRERELEEIR-TKLRLQAQSLNTVGPRLASEYLSPEEM 396
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. UniProt/SwissProt
Match: sp|Q9Z315|SNUT1_MOUSE (U4/U6.U5 tri-snRNP-associated protein 1 OS=Mus musculus OX=10090 GN=Sart1 PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.851e-21
Identity = 64/172 (37.21%), Postives = 95/172 (55.23%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +N G+ + LLL +NKG LE    +   +  P   +  A Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  633 ILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDD---KYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNA 801

HSP 2 Score: 87.0409 bits (214), Expect = 2.879e-16
Identity = 57/161 (35.40%), Postives = 92/161 (57.14%), Query Frame = 3 
Query:  420 DEEFSDK--PMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLR-NKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            +EEF  +   +Y   +L G++VEH    F E    +LTLKDK +L ++ +DVLVN+N++D+ER +KN+   KK   Y  + +    DD L   KP S+L+KYDE ++G + + F L+  G  +  +ER  +  R       +   +  P +AS+YL+P E+
Sbjct:  238 EEEFEQRRQDLYSARDLQGLTVEHAIDSFREGETVVLTLKDKGVL-QDGEDVLVNVNMVDKERADKNVELRKKKPDYLPYAEDESVDD-LAQQKPRSILAKYDEELEGERPHSFRLEQGGMADGLRERELEEIRTKLRLQAQSLSSVGPRLASEYLSPEEM 396
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. UniProt/SwissProt
Match: sp|Q9LFE0|DOT2_ARATH (SART-1 family protein DOT2 OS=Arabidopsis thaliana OX=3702 GN=DOT2 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 3.534e-8
Identity = 46/163 (28.22%), Postives = 75/163 (46.01%), Query Frame = 3 
Query: 1551 EVQINSGIYSTLLLVKNKGYLE----------DKKSQPLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTD------QKLIKMHSKMKS 1991
            EV +  G+   L L+K++G L+          DKK   L+    +   +    +E K R+ DI                           ++E  D+ GR   PKEAFR LSH+FHGK  GK+K  KR+K+  E+ K+  M ++D      Q++ +  +++K+
Sbjct:  620 EVAVGKGLSGALKLLKDRGTLKEKVEWGGRNMDKKKSKLVGIVDDDGGKESKDKESKDRFKDI---------------------------RIERTDEFGRTLTPKEAFRLLSHKFHGKGPGKMKEEKRMKQYQEELKLKQMKNSDTPSQSVQRMREAQAQLKT 755
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. UniProt/SwissProt
Match: sp|O94538|SNU66_SCHPO (U4/U6.U5 tri-snRNP-associated protein snu66 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=snu66 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.577e-6
Identity = 36/108 (33.33%), Postives = 59/108 (54.63%), Query Frame = 3 
Query: 1620 KKSQPLISNPKNKML---RAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFR-ALSHRFHGKTSGKIKSAKRLKKMAEQAK 1931
            KK Q  +S    KM    R QY ++E  R+D           + + E+     + + P++ ++Y D+ G    PKEA++  LSH+FHGK SGK K+ KRL+++ E+ +
Sbjct:  546 KKEQDRLSGKFEKMTQKEREQYAKKENERWD-----------KKIAEIELEQFHDYKPQVDIKYVDEFGVELGPKEAYKYLLSHQFHGKGSGKAKTEKRLRRIVEKER 642
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. TrEMBL
Match: A0A2C9K0C8 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106074971 PE=4 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.296e-32
Identity = 115/294 (39.12%), Postives = 169/294 (57.48%), Query Frame = 3 
Query:   54 ENSEYHGDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIEN---ESSDEEFSDKPM----------YKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGA-YGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            +N++  GDGSSLSIEETN+LR +LGLKPLE +D   ED +  +++D  VH PA+N+G L+  E L  K+   + +RE ++KL + K LG   +   D     +  +KL+ E ++ +      E  DE+F    +          Y + +L G+ VEH   +F+E    +LTLKDK +LDEE+ DVLVN+NI D ER EKN+ NKK    Y  + +   E D   +LKP ++LSKYDE I+G K+  FVL S G Y+   E+     R++  S  +      P + ++YLTP E+
Sbjct:   94 DNNQASGDGSSLSIEETNRLRAKLGLKPLEVSDSKGEDKS--KKSDG-VHVPAVNLGQLRKTESLREKMVNKKTQREMEKKLRTVKGLGDSDSEDEDASAWVLKSRKLQQEKEMAEKRAKMLEEMDEDFGIGSLVEQEFKKDKRYTQQDLRGLKVEHSLEKFEEGRNVVLTLKDKGVLDEEEGDVLVNVNIQDDERAEKNIENKKSKPDYKPYDEP--EYDEYGMLKPSNLLSKYDEEIEGAKKESFVLGSGGSYDAAHEKRMAEIRQALRSQGQSLQMAPPTLLTEYLTPEEM 382

HSP 2 Score: 93.2041 bits (230), Expect = 8.845e-16
Identity = 70/167 (41.92%), Postives = 97/167 (58.08%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDK--KSQPLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMP-FIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTD---QKLIKMHSKMKSENRFCVPLSG 2021
            +L +E   + G+ S L L   KGYLED+  K  P +S  K   L+AQ +  +  RYDD+D+K  +RDR     +  F +K+++ P++KL+Y DD GR    KEAFR LSHRFHGK SGK K+ KR KK+ E+  +  M  TD     L  +  K K+E    + LSG
Sbjct:  609 VLEEEPIAHVGVGSALKLAMKKGYLEDEAPKKLPTLST-KYLELQAQNYTIQDKRYDDLDEKNRKRDRYQGGMITEFKEKDSYKPDVKLDYVDDSGRNLSAKEAFRQLSHRFHGKGSGKKKTEKRSKKVEEELLMKRMSSTDTPLNTLSLLKDKQKTEKSPYIVLSG 774
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. TrEMBL
Match: A0A2C9K0C7 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106074971 PE=4 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.402e-32
Identity = 115/294 (39.12%), Postives = 169/294 (57.48%), Query Frame = 3 
Query:   54 ENSEYHGDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIEN---ESSDEEFSDKPM----------YKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGA-YGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            +N++  GDGSSLSIEETN+LR +LGLKPLE +D   ED +  +++D  VH PA+N+G L+  E L  K+   + +RE ++KL + K LG   +   D     +  +KL+ E ++ +      E  DE+F    +          Y + +L G+ VEH   +F+E    +LTLKDK +LDEE+ DVLVN+NI D ER EKN+ NKK    Y  + +   E D   +LKP ++LSKYDE I+G K+  FVL S G Y+   E+     R++  S  +      P + ++YLTP E+
Sbjct:   94 DNNQASGDGSSLSIEETNRLRAKLGLKPLEVSDSKGEDKS--KKSDG-VHVPAVNLGQLRKTESLREKMVNKKTQREMEKKLRTVKGLGDSDSEDEDASAWVLKSRKLQQEKEMAEKRAKMLEEMDEDFGIGSLVEQEFKKDKRYTQQDLRGLKVEHSLEKFEEGRNVVLTLKDKGVLDEEEGDVLVNVNIQDDERAEKNIENKKSKPDYKPYDEP--EYDEYGMLKPSNLLSKYDEEIEGAKKESFVLGSGGSYDAAHEKRMAEIRQALRSQGQSLQMAPPTLLTEYLTPEEM 382

HSP 2 Score: 93.2041 bits (230), Expect = 8.943e-16
Identity = 70/167 (41.92%), Postives = 97/167 (58.08%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDK--KSQPLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMP-FIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTD---QKLIKMHSKMKSENRFCVPLSG 2021
            +L +E   + G+ S L L   KGYLED+  K  P +S  K   L+AQ +  +  RYDD+D+K  +RDR     +  F +K+++ P++KL+Y DD GR    KEAFR LSHRFHGK SGK K+ KR KK+ E+  +  M  TD     L  +  K K+E    + LSG
Sbjct:  611 VLEEEPIAHVGVGSALKLAMKKGYLEDEAPKKLPTLST-KYLELQAQNYTIQDKRYDDLDEKNRKRDRYQGGMITEFKEKDSYKPDVKLDYVDDSGRNLSAKEAFRQLSHRFHGKGSGKKKTEKRSKKVEEELLMKRMSSTDTPLNTLSLLKDKQKTEKSPYIVLSG 776
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. TrEMBL
Match: A0A3S1HZ78 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_003146 PE=4 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.776e-30
Identity = 109/305 (35.74%), Postives = 156/305 (51.15%), Query Frame = 3 
Query:   48 MSENSEYHGDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIENESSDEEFSDKPMYKRDELFGIS--------------------------VEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGA-YGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLT 881
            +S   +  GDGSSLS+ ETN+LR QLGLKPL+  D    +D++ +++D  VH PAIN+G+ +  ++L  KL     + +R  +       G+  + S DE     +        K +KI+ E    E   K + + DE FGI                           VEH   +F+E    ILTLKDK ILDEE+DDVLVN+NI D ER EKN+ NKK    Y  + +   E D   ++KP ++LSKYDE I+G K+  F L S G Y+   E+     R+   +  +      P IA++Y+T
Sbjct:   83 VSGEPQVSGDGSSLSVVETNRLRAQLGLKPLDIPDAAGGEDSKPKKSDG-VHAPAINMGEKRKADRLREKLSTR--KEKRNIEKRLLDVKGLGDSDSGDEGAAAWV-------LKSRKIQKEKELAEKRAKALEEMDEDFGIGGLVEEEFKKEKDKKQAYRSHDLSGLRVEHAMDKFEEGRGVILTLKDKGILDEEQDDVLVNVNIEDDERAEKNIENKKQKPDYKPYDEP--EYDEYGMMKPNNMLSKYDEEIEGAKKQSFTLGSGGSYDAAHEKRMAEIRQQLRAQGQTLTLAAPTIAAEYMT 375

HSP 2 Score: 80.8777 bits (198), Expect = 6.067e-12
Identity = 62/152 (40.79%), Postives = 89/152 (58.55%), Query Frame = 3 
Query: 1584 LLLVKNKGYLEDK--KSQPLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMP-FIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTD---QKLIKMHSKMKSENRFCVPLSG 2021
            L L  + G++ED+  K  P +S+ K   L+AQ +  +  RYDD+D+K  +R+R     +  F +K T+ PE+KL+Y D+ GR    KEAFR LSHRFHGK SGK K+ KR KK+ E+  +  M  TD     L  +  K ++E    + LSG
Sbjct:  608 LCLYPSTGFIEDESPKKMPGLSS-KYLELQAQNYTIQDKRYDDLDEKNRKRERYQGGMVTDFKEKETYKPEVKLDYVDESGRSLSQKEAFRQLSHRFHGKGSGKKKTEKRGKKVEEELLMKRMSSTDTPLNTLSLLQDKQRTEKSPFIVLSG 758
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. TrEMBL
Match: A0A0X3NFR3 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR146019 PE=4 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 4.609e-27
Identity = 115/290 (39.66%), Postives = 164/290 (56.55%), Query Frame = 3 
Query:   72 GDGSSLSIEETNKLRIQLGLKPLESNDVTNEDD--TEIRRTD-NFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENET--KIQKIENESSDEEFSDKPM----------YKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNK-KMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            G  SSLSIEETNKLR +LGL PL+       DD   +IR +D +FVH PA NI +++  EK++ KL+  RE+RE  EKL   + L +   ++   +++K+ LK+ E     + +K+ +E  DEEF    +          Y   +L G+ VEHKA R  E+G  ILTLKDK +LDE++ DVLVN+NI+D E+ EKN  NK K    G  +   DE D +  L+   VL KYD  IDG K+  FVL + G Y+ E E      ++   S    + + E  +AS+Y +  E+
Sbjct:   53 GSTSSLSIEETNKLRAKLGLAPLDIGTEKKPDDGTAQIRNSDEDFVHVPAKNISEIRRTEKIQEKLRTMREKRELMEKLREAQPLSLGEEVNTLSWVEKMRLKEKERRAAEEREKLLSEM-DEEFGVSQLVQAKISAPKEYASRDLEGLKVEHKADRLIESGPIILTLKDKGVLDEDEGDVLVNVNIIDDEKAEKNRENKRKTAGLGGILQDEDE-DVIMGLRAKGVLEKYDAEIDGPKKQEFVLGAGGSYSSEHELALRRLQQELQSSAHSANDYEVRVASEYFSAAEM 340

HSP 2 Score: 126.331 bits (316), Expect = 3.633e-26
Identity = 75/170 (44.12%), Postives = 110/170 (64.71%), Query Frame = 3 
Query: 1536 GILGDEVQINSGIYSTLLLVKNKGYLEDKKSQPLISNPKNKM--LRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKLI---KMHSKMKSENRFCVPLSGKNS 2030
            G+L DE ++N G+YS LLL + KGY++   +Q   ++  NK+  L A+++ +E +RYDDID K  +R+R + P   F +K+++ PE++L Y DD GR   PKEAFR LSH+FHGK SGK K  KR KK+ E+A +     +D  L    K++ K+KS+    V LSGKN+
Sbjct:  629 GVLDDEPELNQGVYSALLLAQKKGYVD---TQNEATHTVNKLVNLMAKHYVQEDVRYDDIDAKFSKRERYSGPLSEFREKSSYKPEVQLHYVDDMGRALAPKEAFRQLSHKFHGKGSGKKKIEKRTKKIMEEALLRNAQSSDTPLGTTEKLNKKLKSQGMPYVVLSGKNA 795
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. TrEMBL
Match: A0A183SY25 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS9123 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 3.981e-26
Identity = 75/170 (44.12%), Postives = 110/170 (64.71%), Query Frame = 3 
Query: 1536 GILGDEVQINSGIYSTLLLVKNKGYLEDKKSQPLISNPKNKM--LRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKLI---KMHSKMKSENRFCVPLSGKNS 2030
            G+L DE ++N G+YS LLL + KGY++   +Q   ++  NK+  L A+++ +E +RYDDID K  +R+R + P   F +K+++ PE++L Y DD GR   PKEAFR LSH+FHGK SGK K  KR KK+ E+A +     +D  L    K++ K+KS+    V LSGKN+
Sbjct:  605 GVLDDEPELNQGVYSALLLAQKKGYVD---TQNEATHTVNKLVNLMAKHYVQEDVRYDDIDAKFSKRERYSGPLSEFREKSSYKPEVQLHYVDDMGRALAPKEAFRQLSHKFHGKGSGKKKIEKRTKKIMEEALLRNAQSSDTPLGTTEKLNKKLKSQGMPYVVLSGKNA 771

HSP 2 Score: 106.686 bits (265), Expect = 6.130e-20
Identity = 63/150 (42.00%), Postives = 86/150 (57.33%), Query Frame = 3 
Query:  447 YKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNK-KMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            Y   +L G+ VEHKA R  E+G  ILTLKDK +LDE++ DVLVN+NI+D E+ EKN  NK K    G  +   DE D +  L+   VL KYD  IDG K+  FVL + G Y+ E E      ++   S    + + E  +AS+Y +  E+
Sbjct:  168 YASRDLEGLKVEHKADRLIESGPIILTLKDKGVLDEDEGDVLVNVNIIDDEKAEKNRENKRKTAGLGGILQDEDE-DVIMGLRAKGVLEKYDAEIDGPKKQEFVLGAGGSYSSEHELALRRLQQELQSSAHSANDYEVRVASEYFSAAEM 316
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Cavefish
Match: sart1 (spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP [Source:NCBI gene;Acc:103042677])

HSP 1 Score: 110.538 bits (275), Expect = 1.254e-24
Identity = 68/172 (39.53%), Postives = 97/172 (56.40%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +NSG+ + LLL KNKG LE +  +   +  PK  +    Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ KR+KK+ E+A +  M  +D  L     +  K KS+    + LSG    + A
Sbjct:  609 ILDEEPIVNSGLAAALLLCKNKGLLETQVQKVARVRAPKGALPNDNYCIEDKMAIDD---KYSRREEYRGFTQDFKEKDGYKPDVKIEYVDESGRKLTPKEAFRQLSHRFHGKGSGKMKTEKRMKKLEEEALLKKMSSSDTPLGTVALLQEKQKSQKTPYIVLSGSGKSMNA 777

HSP 2 Score: 110.153 bits (274), Expect = 1.778e-24
Identity = 104/289 (35.99%), Postives = 147/289 (50.87%), Query Frame = 3 
Query:   72 GDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIEN---ESSDEEF------------SDKPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            G  +SLSIEETNKLR +LGLKPLE     NE+  E+   +  +    IN   +K + ++  KL   +++R   +KL   K+LG D    +D        +K   E ++ +      E  DEEF            + K  Y   +L G+SV+HK   F E    ILTL+DK +LDE  +DVLVN+ ++D+E+ EKN+  KK        D  +  D +   KP SVLSKYDE IDG K+  F L S G    E+ER     R+S  +  +        +AS+Y TP E+
Sbjct:   98 GGDASLSIEETNKLRAKLGLKPLE----LNENKKELGTKEEPLLAETINPVQIKQQAEIREKLAALKQKRLLNQKLGKVKTLGEDEPWLDDTAAWVERSRKQAKEKELAEKRAKLLEEMDEEFGVSNLVEQEFAQTKKDAYSSRDLKGLSVQHKMESFKEGETVILTLQDKGVLDE-NEDVLVNVGLVDKEKAEKNVELKKKKPEYKPYDEEESVDDMVTFKPKSVLSKYDEEIDGEKKKSFRLSSGGFVGGERERELQAIRESLHNQAQSLEMPALTLASEYYTPQEM 381
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Cavefish
Match: sart1 (spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP [Source:NCBI gene;Acc:103042677])

HSP 1 Score: 110.538 bits (275), Expect = 1.352e-24
Identity = 68/172 (39.53%), Postives = 97/172 (56.40%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSGKNSIIPA 2042
            IL +E  +NSG+ + LLL KNKG LE +  +   +  PK  +    Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ KR+KK+ E+A +  M  +D  L     +  K KS+    + LSG    + A
Sbjct:  602 ILDEEPIVNSGLAAALLLCKNKGLLETQVQKVARVRAPKGALPNDNYCIEDKMAIDD---KYSRREEYRGFTQDFKEKDGYKPDVKIEYVDESGRKLTPKEAFRQLSHRFHGKGSGKMKTEKRMKKLEEEALLKKMSSSDTPLGTVALLQEKQKSQKTPYIVLSGSGKSMNA 770

HSP 2 Score: 98.5969 bits (244), Expect = 6.167e-21
Identity = 64/160 (40.00%), Postives = 89/160 (55.62%), Query Frame = 3 
Query:  420 DEEFSD--KPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            ++EF+   K  Y   +L G+SV+HK   F E    ILTL+DK +LDE  +DVLVN+ ++D+E+ EKN+  KK        D  +  D +   KP SVLSKYDE IDG K+  F L S G    E+ER     R+S  +  +        +AS+Y TP E+
Sbjct:  218 EQEFAQTKKDAYSSRDLKGLSVQHKMESFKEGETVILTLQDKGVLDE-NEDVLVNVGLVDKEKAEKNVELKKKKPEYKPYDEEESVDDMVTFKPKSVLSKYDEEIDGEKKKSFRLSSGGFVGGERERELQAIRESLHNQAQSLEMPALTLASEYYTPQEM 376
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Cavefish
Match: sart1 (spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP [Source:NCBI gene;Acc:103042677])

HSP 1 Score: 109.768 bits (273), Expect = 2.117e-24
Identity = 104/289 (35.99%), Postives = 147/289 (50.87%), Query Frame = 3 
Query:   72 GDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIEN---ESSDEEF------------SDKPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            G  +SLSIEETNKLR +LGLKPLE     NE+  E+   +  +    IN   +K + ++  KL   +++R   +KL   K+LG D    +D        +K   E ++ +      E  DEEF            + K  Y   +L G+SV+HK   F E    ILTL+DK +LDE  +DVLVN+ ++D+E+ EKN+  KK        D  +  D +   KP SVLSKYDE IDG K+  F L S G    E+ER     R+S  +  +        +AS+Y TP E+
Sbjct:   98 GGDASLSIEETNKLRAKLGLKPLE----LNENKKELGTKEEPLLAETINPVQIKQQAEIREKLAALKQKRLLNQKLGKVKTLGEDEPWLDDTAAWVERSRKQAKEKELAEKRAKLLEEMDEEFGVSNLVEQEFAQTKKDAYSSRDLKGLSVQHKMESFKEGETVILTLQDKGVLDE-NEDVLVNVGLVDKEKAEKNVELKKKKPEYKPYDEEESVDDMVTFKPKSVLSKYDEEIDGEKKKSFRLSSGGFVGGERERELQAIRESLHNQAQSLEMPALTLASEYYTPQEM 381

HSP 2 Score: 58.5362 bits (140), Expect = 2.030e-8
Identity = 39/116 (33.62%), Postives = 59/116 (50.86%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSG 1883
            IL +E  +NSG+ + LLL KNKG LE +  +   +  PK  +    Y  E+K+  DD   K  RR+        F +K+ + P++K+EY D+ GR   PKE     + R    + G
Sbjct:  609 ILDEEPIVNSGLAAALLLCKNKGLLETQVQKVARVRAPKGALPNDNYCIEDKMAIDD---KYSRREEYRGFTQDFKEKDGYKPDVKIEYVDESGRKLTPKEFGYKTTQRAWKSSCG 721
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006096.1 (pep scaffold:Pmarinus_7.0:GL480774:18501:31007:-1 gene:ENSPMAG00000005488.1 transcript:ENSPMAT00000006096.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 94.7449 bits (234), Expect = 6.035e-21
Identity = 62/169 (36.69%), Postives = 89/169 (52.66%), Query Frame = 3 
Query: 1548 DEVQINSGIYSTLLLVKNKGYLEDKKSQ-PLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKLIK---MHSKMKSENRFCVPLSGKNSIIPA 2042
            +E  I  G+ S L L   KGYLE + ++   + NP   +  A Y  E+K   DD   K  RR+        F DK  + P++++EY D+ GR   PKEAFR LSHRFHGK SG++K+ KR KK+ E+  +  M  +D  L     +  K K++    + LSG    + A
Sbjct:  249 EEPMITGGLASALKLCNTKGYLETEIAKMARVKNPHKTLPSALYTIEDKNPSDD---KYSRREEYRAYGQEFKDKEHYRPDVRIEYVDETGRKLTPKEAFRQLSHRFHGKGSGRMKTEKRQKKLEEEHMLRKMSSSDTPLGTVALLQEKQKAQKTPFIILSGSGKSMNA 414
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Nematostella
Match: EDO37415 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFV2])

HSP 1 Score: 70.4774 bits (171), Expect = 8.824e-13
Identity = 33/67 (49.25%), Postives = 44/67 (65.67%), Query Frame = 3 
Query: 1764 DKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL 1964
            +K  + P +KLEY D++GR   PKEAFR LSH+FHGKT  K+K+ KR KK+ E+  +  M   D  L
Sbjct:  353 EKEGYKPNVKLEYVDEKGRTLTPKEAFRYLSHKFHGKTPAKMKTEKRTKKILEEMAMQKMSSIDTPL 419
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Medaka
Match: SART1 (spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP [Source:NCBI gene;Acc:101161463])

HSP 1 Score: 105.531 bits (262), Expect = 4.066e-23
Identity = 67/166 (40.36%), Postives = 94/166 (56.63%), Query Frame = 3 
Query: 1539 ILGDEVQINSGIYSTLLLVKNKGYLEDKKSQPL--ISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKL---IKMHSKMKSENRFCVPLSG 2021
            IL +E  +NSG+ + L L  NKG L D + Q +  +   K  +    Y  E+K+ +DD   K  RR+        F DK+ + PEIK+EY D+ GR   PKEAFR LSHRFHGK SGK+K+ +R+KK+ E+A +  M  +D  L     +  K KS+    + LSG
Sbjct:  590 ILDEEPIVNSGLAAALKLCTNKGLL-DTQMQKVARVRATKGSLPNDNYCIEDKMGFDD---KYSRREEYRGFTQDFKDKDGYKPEIKIEYVDESGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLEEEALLKKMSSSDTPLGTVALLQEKQKSQKTPYIVLSG 751

HSP 2 Score: 87.4261 bits (215), Expect = 2.268e-17
Identity = 60/160 (37.50%), Postives = 89/160 (55.62%), Query Frame = 3 
Query:  420 DEEF--SDKPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEI 893
            +EEF  S +  Y   +L G+ V+HK   F E    ILTL+DK +L EEK+DVLVN+ ++D+E+ EKN+  KK           +  + +   K  +VLSKYDE I+G K+  F L + G  + E+ER   + R++  +  +        IAS+Y TP EI
Sbjct:  214 EEEFGQSKQEAYTSRDLKGLKVQHKLESFSEGQSVILTLEDKGVL-EEKEDVLVNVGLIDKEKAEKNVELKKKKPDYKPYQEEESVEDMATFKSHTVLSKYDEEIEGEKKQSFRLSTGGFADGERERELQSVREALRNQAQSLQMPVLTIASEYYTPQEI 372
[Close]
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Planmine SMEST
Match: SMESG000047653.1 (SMESG000047653.1)

HSP 1 Score: 1118.22 bits (2891), Expect = 0.000e+0
Identity = 664/667 (99.55%), Postives = 666/667 (99.85%), Query Frame = 3 
Query:   48 MSENSEYHGDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNFVHTPAINIGDXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXGIDYNLSNDEFXXXXXXXXXENETKIQKIENESSDEEFSDKPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEIXXXXXXXXXXAGGVRTVQSKPAXXXXXXXXXXXXXXXXXXLAEDENGKKSTRGRIVMDFSSDEDDNRPPIVLPELESTAVNLDLKRQLSMLXXXXXXXXXXXXMEIDEIKTESDSKKLVFSNTEEFTKSIFSTLVAKRKVDAEVAAQXXXXXXXXXXXXXXXXXXXXXXXVQSSGWQPEEKIMSQPAQIPSKKKSNYREHTVAPKKKEKLKLTDGILGDEVQINSGIYSTLLLVKNKGYLEDKKSQPLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKLIKMHSKMKSENRFCVPLSGKNSIIPAAL 2048
            MSENSEYHGDGSSLSIEETNKLRIQLGLKPLES+DVTNEDDTEIRRTDNFVHTPAINIGDLKMKEKLENKLKMHREERERKEKLLSEKSLGIDYNLSNDEFLKKVLLKKLENETKIQKIENESSDEE SDKPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEIKEKLKTKKRKAGGVRTVQSKPAKKDSSESETKKSKKEDGELAEDENGKKSTRGRIVMDFSSDEDDNRPPIVLPELESTAVNLDLKRQLSMLKKTKTKVEGVTKME+DEIKTESDSKKLVFSNTEEFTKSIFSTLVAKRKVDAEVAAQVKEEPVTKPTPPAPKKIEIKEEPVQSSGWQPEEKIMSQPAQIPSKKKSNYREHTVAPKKKEKLKLTDGILGDEVQINSGIYSTLLLVKNKGYLEDKKSQPLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKLIKMHSKMKSENRFCVPLSGKNSIIPAAL
Sbjct:    1 MSENSEYHGDGSSLSIEETNKLRIQLGLKPLESSDVTNEDDTEIRRTDNFVHTPAINIGDLKMKEKLENKLKMHREERERKEKLLSEKSLGIDYNLSNDEFLKKVLLKKLENETKIQKIENESSDEELSDKPMYKRDELFGISVEHKASRFDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGAFVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERTEDTYRKSFFSGTKHSINTEPIIASDYLTPLEIKEKLKTKKRKAGGVRTVQSKPAKKDSSESETKKSKKEDGELAEDENGKKSTRGRIVMDFSSDEDDNRPPIVLPELESTAVNLDLKRQLSMLKKTKTKVEGVTKMEVDEIKTESDSKKLVFSNTEEFTKSIFSTLVAKRKVDAEVAAQVKEEPVTKPTPPAPKKIEIKEEPVQSSGWQPEEKIMSQPAQIPSKKKSNYREHTVAPKKKEKLKLTDGILGDEVQINSGIYSTLLLVKNKGYLEDKKSQPLISNPKNKMLRAQYFQEEKIRYDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFRALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKLIKMHSKMKSENRFCVPLSGKNSIIPAAL 667
The following BLAST results are available for this feature:
BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SART15.548e-2237.21spliceosome associated factor 1, recruiter of U4/U... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
F19F10.95.383e-1430.41pep chromosome:WBcel235:V:7571950:7575715:-1 gene:... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG66862.786e-2342.86gene:FBgn0032388 transcript:FBtr0089573[more]
CG66862.800e-2342.86gene:FBgn0032388 transcript:FBtr0089572[more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sart19.422e-2439.31spliceosome associated factor 1, recruiter of U4/U... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSXETT00000022048.17.458e-2735.93SART1, U4/U6.U5 tri-snRNP-associated protein 1 [So... [more]
ENSXETT00000022057.17.722e-2735.93SART1, U4/U6.U5 tri-snRNP-associated protein 1 [So... [more]
ENSXETT00000022014.15.169e-2337.79SART1, U4/U6.U5 tri-snRNP-associated protein 1 [So... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Sart16.934e-2237.21squamous cell carcinoma antigen recognized by T ce... [more]
Sart12.350e-771.43squamous cell carcinoma antigen recognized by T ce... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O43290|SNUT1_HUMAN2.664e-2137.21U4/U6.U5 tri-snRNP-associated protein 1 OS=Homo sa... [more]
sp|Q5XIW8|SNUT1_RAT4.221e-2137.21U4/U6.U5 tri-snRNP-associated protein 1 OS=Rattus ... [more]
sp|Q9Z315|SNUT1_MOUSE4.851e-2137.21U4/U6.U5 tri-snRNP-associated protein 1 OS=Mus mus... [more]
sp|Q9LFE0|DOT2_ARATH3.534e-828.22SART-1 family protein DOT2 OS=Arabidopsis thaliana... [more]
sp|O94538|SNU66_SCHPO1.577e-633.33U4/U6.U5 tri-snRNP-associated protein snu66 OS=Sch... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2C9K0C81.296e-3239.12Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A2C9K0C71.402e-3239.12Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A3S1HZ781.776e-3035.74Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
A0A0X3NFR34.609e-2739.66Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A183SY253.981e-2644.12Uncharacterized protein OS=Schistocephalus solidus... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
sart11.254e-2439.53spliceosome associated factor 1, recruiter of U4/U... [more]
sart11.352e-2439.53spliceosome associated factor 1, recruiter of U4/U... [more]
sart12.117e-2435.99spliceosome associated factor 1, recruiter of U4/U... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000006096.16.035e-2136.69pep scaffold:Pmarinus_7.0:GL480774:18501:31007:-1 ... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO374158.824e-1349.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SART14.066e-2340.36spliceosome associated factor 1, recruiter of U4/U... [more]
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BLAST of U4/U6.U5 tri-snRNP-associated protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000047653.10.000e+099.55SMESG000047653.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009928 ID=SMED30009928|Name=U4/U6.U5 tri-snRNP-associated protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=2136bp
TTTAATTGTTTTAATAATATTAATTTAAATCGGTTTATATTTTGAGAATG
TCTGAAAATTCTGAATATCATGGTGATGGATCCTCATTGAGTATAGAAGA
AACAAATAAGTTACGTATTCAATTAGGTTTGAAACCTTTGGAGTCAAATG
ATGTTACCAATGAAGACGATACAGAAATTAGAAGAACAGATAATTTCGTA
CATACACCAGCCATCAATATAGGTGATCTTAAAATGAAGGAAAAACTTGA
AAATAAACTAAAAATGCATCGGGAGGAAAGGGAGAGAAAAGAGAAATTAT
TGTCTGAAAAGTCATTGGGCATTGACTACAATTTGTCGAATGACGAATTT
TTGAAAAAAGTTTTATTGAAGAAATTAGAAAATGAAACTAAAATTCAAAA
AATTGAAAACGAATCCTCTGATGAAGAATTTTCAGATAAACCAATGTACA
AACGAGATGAGTTATTTGGAATATCCGTTGAACACAAAGCTAGTAGATTT
GACGAAGCTGGTATAACTATCCTAACTCTAAAAGACAAAAACATTCTGGA
CGAAGAAAAAGACGATGTTCTTGTAAATATCAATATCTTGGATAGAGAAA
GAGAGGAAAAGAATTTAAGAAATAAAAAAATGGGAGCCTATGGAGCATTT
GTTGATTCTGGAGATGAAGATGATCCTTTAACATTGTTAAAACCGCCGAG
TGTTTTATCGAAATACGACGAAGTAATCGACGGAGTGAAGCGCAATCGAT
TCGTATTAGATTCCAAAGGAAAATATAACATGGAACAAGAAAGAACGGAA
GATACTTATCGAAAATCTTTTTTTTCTGGAACAAAACACAGCATCAATAC
AGAACCAATAATAGCTAGTGATTATTTGACTCCGCTAGAAATCAAAGAAA
AATTAAAGACTAAGAAACGTAAAGCCGGTGGTGTAAGAACCGTCCAATCG
AAACCCGCCAAAAAAGATTCCAGTGAATCAGAAACGAAAAAGTCCAAAAA
AGAAGACGGCGAATTAGCCGAAGATGAAAACGGGAAAAAATCAACTAGAG
GACGAATTGTGATGGATTTTTCTAGCGATGAAGATGATAACCGGCCACCG
ATAGTGTTACCGGAACTCGAATCGACTGCAGTGAATTTGGATTTAAAGCG
CCAATTGTCGATGTTGAAGAAAACTAAAACAAAAGTCGAAGGAGTAACGA
AAATGGAAATAGATGAAATAAAAACCGAATCGGATTCAAAGAAATTAGTG
TTCTCCAACACTGAAGAGTTTACAAAATCGATTTTCAGCACTTTGGTGGC
CAAACGGAAAGTCGATGCCGAGGTGGCTGCACAAGTAAAGGAAGAGCCAG
TAACTAAACCGACACCTCCAGCTCCGAAGAAAATCGAAATTAAAGAAGAA
CCAGTACAATCGAGTGGATGGCAACCAGAAGAAAAGATAATGTCACAACC
TGCTCAAATACCTTCGAAGAAAAAATCAAATTATCGAGAACATACTGTTG
CTCCCAAGAAAAAAGAGAAGCTCAAATTAACAGATGGAATCCTTGGTGAT
GAAGTGCAAATCAATTCGGGAATATATTCGACATTGTTGCTTGTTAAAAA
CAAAGGCTACCTAGAAGATAAAAAGTCTCAGCCGTTGATATCGAACCCGA
AAAATAAAATGCTACGAGCACAGTATTTCCAGGAAGAGAAAATACGATAT
GACGATATCGATCAAAAGTGCCCGCGTCGCGACAGGCGGAACCTACCAGA
AATGCCGTTTATCGACAAAAACACTTTCGATCCGGAAATCAAGCTCGAGT
ATCACGATGACCAAGGACGCCCTCAACCGCCGAAAGAAGCATTCCGAGCT
CTAAGTCATCGATTTCATGGGAAAACATCGGGAAAGATCAAATCAGCGAA
ACGCTTGAAGAAAATGGCCGAGCAGGCTAAAATCAACACAATGGGAGATA
CGGACCAGAAATTGATCAAGATGCACAGTAAAATGAAGAGTGAAAATAGA
TTTTGTGTGCCATTGAGTGGGAAAAATTCCATAATCCCAGCTGCTTTAAA
GAAAGGCGAAAAGAAATAAACAAAACTGAAAAATGATAATTTTATCACAA
TTTGGCATTTTGAAATAAATTTGTTTCATGTTTTTC
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protein sequence of SMED30009928-orf-1

>SMED30009928-orf-1 ID=SMED30009928-orf-1|Name=SMED30009928-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=674bp
MSENSEYHGDGSSLSIEETNKLRIQLGLKPLESNDVTNEDDTEIRRTDNF
VHTPAINIGDLKMKEKLENKLKMHREERERKEKLLSEKSLGIDYNLSNDE
FLKKVLLKKLENETKIQKIENESSDEEFSDKPMYKRDELFGISVEHKASR
FDEAGITILTLKDKNILDEEKDDVLVNINILDREREEKNLRNKKMGAYGA
FVDSGDEDDPLTLLKPPSVLSKYDEVIDGVKRNRFVLDSKGKYNMEQERT
EDTYRKSFFSGTKHSINTEPIIASDYLTPLEIKEKLKTKKRKAGGVRTVQ
SKPAKKDSSESETKKSKKEDGELAEDENGKKSTRGRIVMDFSSDEDDNRP
PIVLPELESTAVNLDLKRQLSMLKKTKTKVEGVTKMEIDEIKTESDSKKL
VFSNTEEFTKSIFSTLVAKRKVDAEVAAQVKEEPVTKPTPPAPKKIEIKE
EPVQSSGWQPEEKIMSQPAQIPSKKKSNYREHTVAPKKKEKLKLTDGILG
DEVQINSGIYSTLLLVKNKGYLEDKKSQPLISNPKNKMLRAQYFQEEKIR
YDDIDQKCPRRDRRNLPEMPFIDKNTFDPEIKLEYHDDQGRPQPPKEAFR
ALSHRFHGKTSGKIKSAKRLKKMAEQAKINTMGDTDQKLIKMHSKMKSEN
RFCVPLSGKNSIIPAALKKGEKK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR005011SNU66/SART1
Vocabulary: biological process
TermDefinition
GO:0000398mRNA splicing, via spliceosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 64..87
NoneNo IPR availableCOILSCoilCoilcoord: 102..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 451..484
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 288..351
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 303..351
IPR005011SNU66/SART1 familyPFAMPF03343SART-1coord: 13..625
e-value: 4.2E-92
score: 309.7
IPR005011SNU66/SART1 familyPANTHERPTHR14152SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTEScoord: 10..648