SMED30009883

Overview
NameSMED30009883
Smed IDSMED30009883
Length (bp)950
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009883 (SMED30009883) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009883 (SMED30009883) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009883 (SMED30009883) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009883 (SMED30009883) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30009883

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30009883h1SMcG0018158 dd_Smed_v4_30795_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009883 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002673:706..1549 +3007
Homology
BLAST of SMED30009883 vs. TrEMBL
Match: C3YAR7 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_82444 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 6.306e-10
Identity = 62/221 (28.05%), Postives = 96/221 (43.44%), Query Frame = -2
Query:  110 NKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVS--PMFSSDETVYPSEKSLRQLLDLNYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEF--MAESRN 760
            + +LD G GTG    ++       S +G D S  F+ YA  RT  SS  + Y   D  DP  +K   R       VVS   +H++ D     +   SCL    E V V V   S+   +      +  +W IY  KD   +  P  SSD  +    +S R +    Y++         + + S +QL+ V R  LP +  IP+  + EF+++   +AE R+
Sbjct:   34 DAVLDAGCGTGELCSYISKQPGVASVVGFDLSPDFISYA--RTNNSSPNVRYHVADAADPSTIKPEWRG--AFSKVVSFFVLHWVKDKATALKALNSCLKPGGEIVFVCVSDKSK-FHEVPLELSSLPKWSIYL-KDAAPALFPWPSSDIMITNEHRSSRLMEACGYEVIACDIKKYPHCYISRDQLRGVIRPTLPHLSYIPEDKQEEFMEDLEKLAEERH 248          
BLAST of SMED30009883 vs. TrEMBL
Match: A0A363U9M4 (SAM-dependent methyltransferase OS=Anaerolineales bacterium OX=2073117 GN=C3F07_05865 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.557e-8
Identity = 41/144 (28.47%), Postives = 69/144 (47.92%), Query Frame = -2
Query:  437 NGDEYDEFMG----YVNKTSKNAIKYNVVPLKKSYNKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCL-TGDVEHVHV 853
            NGD Y+++MG    YV +   N +  +      +  + LD+G GTG+ +  ++D   P S  G+D+SEGF+E+AR+R     V    E  +         +   +   D VVS   ++F+ +P + F        +G V  V+V
Sbjct:   11 NGDLYEDYMGRWSRYVAREFINWLSLS------AGGEWLDVGCGTGALSQTVLDLAKPASIKGIDRSEGFVEFARERVGDERVRFEVEDAE--------TITDANESFDAVVSGLVLNFIPNPERAFAGMTRVTRSGGVVAVYV 140          
BLAST of SMED30009883 vs. TrEMBL
Match: A0A293LRN6 (Methyltransf_25 domain-containing protein (Fragment) OS=Ornithodoros erraticus OX=265619 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.010e-6
Identity = 58/241 (24.07%), Postives = 103/241 (42.74%), Query Frame = -2
Query:   38 LDIGSGTGSFT-DFLVDFYHP-KSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDLNYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLCRN-TENKVFWQYDLFILHAKTGP 751
            LDIG G G+ T D +++   P +  +G+D S   ++YARQ +   +V   Y+  D  +   +    +++     V S  + H+L D  + F+     +T + E +   V  +     D  E       W+ Y  + Y +  +  S+  V   +    QL     K H  +   +  +  S + L  +Y  LLP+   IP   K E + +FM      C + +  K+     LF++HA+  P
Sbjct:   48 LDIGCGDGAVTRDVVLENSMPCRRLVGVDISSDMVQYARQHSGHPNV--TYDVLDICE--DVTTFRKQYGQFRRVYSFFAFHWLEDQERGFKNIADLMTPNGECLLAYVGSVPS--FDVWEELSKVPRWRAY--RGYLLRAVPQSNSIVNQRDYMEIQLKTAGLKAHTCEVLRIQWKMESEQALADMYEPLLPVPDDIPPEDKEELMRDFMTGMEKYCTDKSTGKIVLPLSLFLIHARKLP 280          
BLAST of SMED30009883 vs. TrEMBL
Match: A0A2T1D8B7 (SAM-dependent methyltransferase OS=filamentous cyanobacterium CCP2 OX=2107700 GN=C7B76_11165 PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.169e-6
Identity = 55/225 (24.44%), Postives = 100/225 (44.44%), Query Frame = -2
Query:  104 KSYNKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCL-TGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDLN-YKIHLEKFFPVSNEFYSYEQLK-YVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLC 769
            + +  +LDIG G G  T  L         +G+D S+  + +A  + Y     +N  +  + D +Q    LR     D+V S+ ++H+++D + + +     L T     + +  R    E++  +E   +   W+ YF+        FS     Y  E+  R L  +N Y + +E   P +  + S E+L  ++  + LP  Q +P   + +FIDE +A    LC
Sbjct:   32 QGHESILDIGCGNGKITAELSSLVPNGVVVGIDSSDTMIRFATDK-YPPERFLNL-RFIWADARQ----LRFQNQFDIVFSNAALHWVDDHLSVLKGISQSLKTKGRVLLQMGGRGNIPEMLQAIEIVTSSMRWQTYFQH-------FSFPYHFYGLEEYQRWLEVVNLYPVRIE-LIPKTITYRSSEELAGWIRTTWLPYTQQVPQQWREDFIDELVACYLRLC 242          
BLAST of SMED30009883 vs. TrEMBL
Match: A0A1I8NVH2 (Methyltransf_25 domain-containing protein OS=Stomoxys calcitrans OX=35570 GN=106087705 PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.222e-6
Identity = 67/260 (25.77%), Postives = 111/260 (42.69%), Query Frame = -2
Query:   47 NKLLDIGSGTGSFTDFLVDFYHP------KSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHF-------LNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQ--VSPMFSSDETVYPSEKSLRQLLDLNYKIHLE-KFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLCRNT------ENKVFWQYDLFILHAK 760
            + L+D+GSG G   D L+D+ +P         +G D S   ++YA QR Y    +  +   D G PK L + L+     D V S   +H+       LN+  KL         GD   + +S    S  + D   +     +W  Y   D Q  VSP+   D+T    +    Q+++      +E    P S  + + E  K   +S+  L++ IP   ++EF+D+F+A        T      ++K   +YDL I + +
Sbjct:   37 DSLIDVGSGPG---DVLIDYIYPLMPANFTKIIGSDISADMVDYA-QRFYHFVEKCEFLTLDIGSPKPLPQGLQGQ--FDHVTSMLCLHWVQNHRLALNNMFKLLRPS----GGDCFLIFMS----SHTIFDAYLHINKDPKWSPYITTDVQRFVSPLHGCDQT----KDVFTQMMEDEGFCEVEVDLRPRSINYGTVENFKENIKSVCGLIEYIPKSKQDEFMDDFVALMSEYAAKTGDLSDSQSKCSMKYDLLIAYGR 278          
BLAST of SMED30009883 vs. Planmine SMEST
Match: SMESG000038735.1 (SMESG000038735.1)

HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = -2
Query:   35 MHFFAINGDEYDEFMGYVNKTSKNAIKYNVVPLKKSYNKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDLNYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLCRNTENKVFWQYDLFILHAKTGPT 871
            MHFFAINGDEYDEFMGYVNKTSKNAIKYNVVPLKKSYNKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDLNYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLCRNTENKVFWQYDLFILHAKTGPT
Sbjct:    1 MHFFAINGDEYDEFMGYVNKTSKNAIKYNVVPLKKSYNKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDLNYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLCRNTENKVFWQYDLFILHAKTGPT 279          
BLAST of SMED30009883 vs. Planmine SMEST
Match: SMESG000038734.1 (SMESG000038734.1)

HSP 1 Score: 254.988 bits (650), Expect = 6.213e-84
Identity = 116/277 (41.88%), Postives = 178/277 (64.26%), Query Frame = -2
Query:   41 MHFFAINGDEYDEFMGYVNKTSKNAIKYNVVPLKKSYNKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDLNYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLCRNTENKVFWQYDLFILHAKTG 871
            M F A+  ++YD++        K  +   V+P KK+Y K+LD+G GTG+ TDF ++F+ P+SF+G+D +E FL + RQR Y S++EI YEKC+  D ++LK++L+ HPDIDLVVSS  +H++NDPI LF+T   CL G VEH H+SVR +  +LID +  Y NH +WK Y+E   Q+ P+F+  E ++ S++   ++LD  ++I+L+   P+ ++F + E      + +LP  + IP HL +EFI+EF+  S    RN   +  W YD F+ HAK  
Sbjct:    1 MSFLALTEEDYDKYGQITQSFVKGMMTKFVIPKKKTYKKMLDLGCGTGNITDFFIEFFKPESFIGIDTNESFLNFGRQRPYISTIEIKYEKCNLNDMEELKEILKNHPDIDLVVSSFVLHWINDPITLFQTLSLCLNGHVEHAHISVRNIPIDLIDLIGEYENHPKWKTYYEDKCQIPPLFNRIEPIFSSKEFYEKILDHKFEIYLQTSIPICHQFVTDELFTIFIKFILPFTKHIPKHLHDEFINEFVNNSSGFYRNDNGRYAWDYDFFVCHAKNS 277          
BLAST of SMED30009883 vs. Planmine SMEST
Match: SMESG000038731.1 (SMESG000038731.1)

HSP 1 Score: 251.906 bits (642), Expect = 8.267e-83
Identity = 124/280 (44.29%), Postives = 190/280 (67.86%), Query Frame = -2
Query:   47 MHFFAINGDEYDEFMG-----YVNKTSKNAIKYNVVPLKKSYNKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDLNYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLCRNTENKVFWQYDLFILHAK 871
            M F A++G EY E  G     YV+K     I+  V+P K +Y KLL++G GTG+ TDF ++++ P+SFLG+D+ E +LEYAR+   KSS+EI YEKCD  + ++LK++L+K+ D+DLV+SS  +H+L DPI+LF+T  S L   VEH H+SV    +ELID VE Y  H  WK+Y++   ++ P+ S  + +YPS+  ++ +LD  ++I+LE+  PV+N F S E  +  ++ LLP +  IP HL+ EF+ EF+++SR  CR+ ++KV W+Y+LF+ H K
Sbjct:    1 MSFSALSGSEY-ELCGKNTKLYVSK----MIQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWLEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGELFHKFLLPFLNFIPCHLREEFMKEFLSDSRKACRSVDDKVTWKYNLFLFHTK 275          
The following BLAST results are available for this feature:
BLAST of SMED30009883 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
C3YAR76.306e-1028.05Uncharacterized protein OS=Branchiostoma floridae ... [more]
A0A363U9M43.557e-828.47SAM-dependent methyltransferase OS=Anaerolineales ... [more]
A0A293LRN62.010e-624.07Methyltransf_25 domain-containing protein (Fragmen... [more]
A0A2T1D8B72.169e-624.44SAM-dependent methyltransferase OS=filamentous cya... [more]
A0A1I8NVH22.222e-625.77Methyltransf_25 domain-containing protein OS=Stomo... [more]
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BLAST of SMED30009883 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009883 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000038735.10.000e+0100.00SMESG000038735.1[more]
SMESG000038734.16.213e-8441.88SMESG000038734.1[more]
SMESG000038731.18.267e-8344.29SMESG000038731.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009883 ID=SMED30009883|Name=SMED30009883|organism=Schmidtea mediterranea sexual|type=transcript|length=950bp
TTGTCTTTCAATAATTATTTATTTCCAAAAGCTATGTTGGTCCGGTTTTC
GCATGTAAAATAAACAAATCATATTGCCAAAATACTTTATTCTCAGTGTT
TCTACACAGGTTCCTGGACTCGGCCATAAATTCGTCGATGAATTCGTTCT
TAAGATGATCAGGAATGATTTGCATCAATGGCAAAAGACTCCTATAAACA
TATTTCAATTGTTCATAGGAATAGAATTCATTTGAAACTGGAAAAAACTT
TTCTAAATGAATTTTATAGTTTAAATCCAAAAGTTGTCGAAGGGATTTTT
CTGAAGGGTAAACCGTTTCATCTGAACTAAACATTGGTGAAACTTGATAA
TCTTTCTCAAAATAGATTTTCCATTCCTGATGATTTCGATAGTTTTCAAC
AAAATCAATTAGTTCTTGAGAGAGTTTTCGTACCGAAACATGAACATGTT
CAACATCCCCAGTTAGACATGATATAAATGTTTCAAATAGCTTTATCGGA
TCATTAAGGAAGTGTATACTATGTGAACTAACAACAAGGTCAATGTCTGG
ATGTTTTCTTAATACTTTCTTCAACTGTTTTGGATCGCCGAAATCACATT
TTTCATAATTTATTTCAACCGATGACTTATAGGTTCGTTGTCTGGCATAT
TCGAGAAATCCTTCGCTTTGATCAAGTCCAAGGAATGATTTTGGGTGATA
AAAATCTACCAGGAAATCCGTAAAACTGCCAGTCCCACTGCCAATATCCA
AAAGTTTATTATAGCTTTTCTTTAGTGGGACAACATTATATTTTATTGCA
TTTTTTGATGTTTTATTCACATAACCCATGAACTCATCATACTCATCGCC
ATTTATCGCAAAGAAATGCATTGTATCAAAAAAATGTATATTGTGCGCTT
AAATTTTGTTTTAGATGCGCGTATGTGAAATTTTGCTATATTTTTAATGC
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protein sequence of SMED30009883-orf-1

>SMED30009883-orf-1 ID=SMED30009883-orf-1|Name=SMED30009883-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=280bp
MHFFAINGDEYDEFMGYVNKTSKNAIKYNVVPLKKSYNKLLDIGSGTGSF
TDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQL
KKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKL
SQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDL
NYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMA
ESRNLCRNTENKVFWQYDLFILHAKTGPT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR041698Methyltransf_25
IPR029063SAM-dependent_MTases
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 3..221
e-value: 1.1E-16
score: 62.8
NoneNo IPR availablePANTHERPTHR42912FAMILY NOT NAMEDcoord: 36..130
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 39..136
e-value: 1.14938E-6
score: 44.3431
IPR041698Methyltransferase domain 25PFAMPF13649Methyltransf_25coord: 41..136
e-value: 1.4E-10
score: 41.7
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILYSSF53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 3..139