Lipopolysaccharide choline phosphotransferase protein

Overview
NameLipopolysaccharide choline phosphotransferase protein
Smed IDSMED30009860
Length (bp)1236
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Lipopolysaccharide choline phosphotransferase protein (SMED30009860) t-SNE clustered cells

Violin plots show distribution of expression levels for Lipopolysaccharide choline phosphotransferase protein (SMED30009860) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Lipopolysaccharide choline phosphotransferase protein (SMED30009860) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Lipopolysaccharide choline phosphotransferase protein (SMED30009860) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009860

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30009860h1SMcG0000248 SmedASXL_011969SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30009860h1SMcG0000248 dd_Smed_v4_7880_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30009860h1SMcG0000248 dd_Smed_v4_7880_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009860h1SMcG0000248 Contig5618GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009860h1SMcG0018726 Contig5618GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30009860h1SMcG0000248 OX_Smed_1.0.20669ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A1I8J1S0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 1.508e-56
Identity = 105/263 (39.92%), Postives = 147/263 (55.89%), Query Frame = 3 
Query:  435 RNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHK--SGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLP--------HENGVREILKYKDIEIT-DIVIPEPKYAVSETFMLSLKPGFSMP 1190
            R   +K F+  +T  ER ++ RLL+  D   +  NVTYI+YGGTLLGS RHH L+PWDDDVDL++    R  +  +    +PE      GGHDK +    S + + + W WPYLD+S++ EN TH+ D + TY  W+ YPK +V P H RP  +  LP P+DT AFL  TY     C    YSHM+E G PSG   C +++   PFV   P         ++ V E L   + ++   + + EP +A++E F L L  GF+ P
Sbjct:   76 RVKNVKLFKPFVTKQERWVMLRLLKRIDHVFRKYNVTYIIYGGTLLGSHRHHGLVPWDDDVDLLVNKTVRRHLPAMLAAAAPELVLQEAGGHDKIYDGGLSRRTSTYPWRWPYLDVSFFEENSTHLWDHMATYSHWFVYPKWLVLPTHARPFEHLRLPVPRDTLAFLQLTYGRTGTCLTSAYSHMIEDGMPSGRRPCLSLNGSVPFVHRKPCTTSSNQRTDSCVLETLVLGNRQLKPAVAVAEPSFAINEPFSLKLVHGFTQP 338
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A1I8G4T0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 1.801e-56
Identity = 105/258 (40.70%), Postives = 145/258 (56.20%), Query Frame = 3 
Query:  450 KPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHK--SGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLP--------HENGVREILKYKDIEIT-DIVIPEPKYAVSETFMLSLKPGFSMP 1190
            KPF   +T  ER ++ RLL+  D   +  NVTYI+YGGTLLGS RHH L+PWDDDVDL++    R  +  +    +PE      GGHDK +    S + + + W WPYLD+S++ EN TH+ D + TY  W+ YPK +V P H RP  +  LP P+DT AFL  TY     C    YSHM+E G PSG   C +++   PFV   P         ++ V E L   + ++   + + EP +A++E F L L  GF+ P
Sbjct:  126 KPF---VTKQERWVMLRLLKRIDHVFRKYNVTYIIYGGTLLGSHRHHGLVPWDDDVDLLVNKTVRRHLPAMLAAAAPELVLQEAGGHDKIYDGGLSRRTSTYPWRWPYLDVSFFEENSTHLWDHMATYSHWFVYPKWLVLPTHARPFEHLRLPVPRDTLAFLQLTYGRTGTCLTSAYSHMIEDGMPSGRRPCLSLNGSVPFVHRKPCTTSSNQRTDSCVLETLVLGNRQLKPAVAVAEPSFAINEPFSLKLVHGFTQP 380
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A1S8WS50 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=X801_06824 PE=4 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 2.540e-55
Identity = 111/345 (32.17%), Postives = 177/345 (51.30%), Query Frame = 3 
Query:  111 KWFLLLVTF-CVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKM------VNSNKQNEKNKTHDVNFVKKXXXXXXXXXXXXXXRNSKMKP-FQIQMTFDERKMVYRLLELFDQTMKNNNV--TYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHK-----------SGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVRE 1082
            K F  LV+  C+G+++I+    Q      + F      K       +S       VQ       R + A +L KM      V       +N   +V                     +++ P F+  M+  +RK+  RLL++F +TM  N +   +++YGGTLLGS+RHHD IPWD+D+D+++ V  R K++E+    +P+Y F      DK +                +   +W WP+LD+SY+  N TH+ +I  +YG++Y +P ++VFPLH RPL  Y  P+P+DT+ F   TY+ G+ C + GYSH+ E  GPSG++ C+ ++ K+ FVEH+  +NG  E
Sbjct:   12 KIFRFLVSLSCLGILVILIQQFQSHDVIHIVFSE----KYGNTLDFVSVRSNEFRVQPFWSQPNRSVFAPDLAKMSWPKDPVAPYPAGRRNSNGEV---------------------AQLPPLFEPVMSIAQRKLSKRLLQIFSETMFANGLGDRFMLYGGTLLGSYRHHDFIPWDEDIDVLVDVLIRDKVRELLSGLAPQYNFYQGYSRDKLYGPLIDGAENELDVEKSRPAANWGWPFLDISYFRVNGTHVMEITMSYGRYYVWPYDVVFPLHFRPLGKYWYPAPRDTWLFNRLTYDPGAMCVIPGYSHVKESRGPSGSMPCRDLASKYAFVEHVACKNGTSE 331
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A074ZZ74 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_02483 PE=4 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 3.571e-55
Identity = 111/345 (32.17%), Postives = 177/345 (51.30%), Query Frame = 3 
Query:  111 KWFLLLVTF-CVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKM------VNSNKQNEKNKTHDVNFVKKXXXXXXXXXXXXXXRNSKMKP-FQIQMTFDERKMVYRLLELFDQTMKNNNV--TYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHK-----------SGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVRE 1082
            K F  LV+  C+G+++I+    Q      + F      K       +S       VQ       R + A +L KM      V       +N   +V                     +++ P F+  M+  +RK+  RLL++F +TM  N +   +++YGGTLLGS+RHHD IPWD+D+D+++ V  R K++E+    +P+Y F      DK +                +   +W WP+LD+SY+  N TH+ +I  +YG++Y +P ++VFPLH RPL  Y  P+P+DT+ F   TY+ G+ C + GYSH+ E  GPSG++ C+ ++ K+ FVEH+  +NG  E
Sbjct:   17 KIFRFLVSLSCLGILVILIQQFQSHDVIHIVFSE----KYGNTLDFVSVRSNEFRVQPFWSQPNRSVFAPDLAKMSWPKDPVAPYPAGRRNSNGEV---------------------AQLPPLFEPVMSIAQRKLSKRLLQIFSETMFANGLGDRFMLYGGTLLGSYRHHDFIPWDEDIDVLVDVLIRDKVRELLSGLAPQYNFYQGYSRDKLYGPLIDGAENELDVEKSRPAANWGWPFLDISYFRVNGTHVMEITMSYGRYYVWPYDVVFPLHFRPLGKYWYPAPRDTWLFNRLTYDPGAMCVIPGYSHVKESRGPSGSMPCRDLASKYAFVEHVACKNGTSE 336
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A3R7H1K8 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_3783s PE=4 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 9.480e-53
Identity = 109/339 (32.15%), Postives = 173/339 (51.03%), Query Frame = 3 
Query:  111 KWFLLLVTF-CVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKMVNSNKQNEKNKTHDVNFVKKXXXXXXXXXXXXXXRNSKMKP-FQIQMTFDERKMVYRLLELFDQTMKNNNVT--YIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHK-----------SGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVRE 1082
            K FL LV+  C+G+ +I+   EQF     +    +   K       +S       VQ       R +   +L  M               N+ K           + +   +K+ P F+  M+  +R +  RLL++F +TM  N +   +++YGGTLLGS+RHHD IPWD+D+D+++ V  RGK++E+  + +P Y F      DK +                +   +W WP+LD+SY+  N TH  +I  +YG+ Y +P ++VFPL+ RPL  +  P+P+DT+ F   TY  G+ C + GYSH+ E  GPSG++ C+ ++ K+ FVEH+   NG  E
Sbjct:   17 KIFLFLVSISCLGIFVILI--EQFQSHDVIHI--VFSEKYGNTPGFVSVKSNEFRVQPFGSQPHRSVSLPDLATM---------------NWPKDPLAPYPAGRRNSKGEVAKLPPLFEPVMSIAQRNLSKRLLQVFSETMFANGLGDRFMLYGGTLLGSYRHHDFIPWDEDIDVLVDVLIRGKVRELLSRLAPRYNFYQGYSRDKLYGPLINDTENELDVEKSRPAANWGWPFLDISYFRVNGTHAMEITMSYGRHYMWPYDVVFPLYFRPLGRHWYPAPRDTWLFNRLTYAPGTMCVIPGYSHVKESSGPSGSMPCRDLASKYAFVEHVACNNGTSE 336
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Match: EDO44531 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV89])

HSP 1 Score: 57.3806 bits (137), Expect = 3.608e-9
Identity = 31/96 (32.29%), Postives = 48/96 (50.00%), Query Frame = 3 
Query:  489 MVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDL 776
            +  R++ LFD   K +N++Y +  GTLLG+ RH   IPWD D D+ IP+    K  E+  K  P   F+     D +     K+   S + P + +
Sbjct:  183 ITTRMMRLFDLIAKKHNISYWLTSGTLLGAARHRGNIPWDTDNDIDIPLADYIKFFEVAAKELPSDMFLQNSISDPYLRPDNKQYAASITHPKVGI 278
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Match: EDO35576 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SL37])

HSP 1 Score: 55.4546 bits (132), Expect = 1.865e-8
Identity = 21/50 (42.00%), Postives = 34/50 (68.00%), Query Frame = 3 
Query:  489 MVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVK 638
            ++ R+L +FDQ  ++N + Y +  GTLLG+ RH   IPWD D D+ +P++
Sbjct:  126 VLTRMLRIFDQIARSNGLKYWLTSGTLLGAVRHRGFIPWDGDADIEMPLE 175
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Match: EDO45540 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSB7])

HSP 1 Score: 54.299 bits (129), Expect = 2.129e-8
Identity = 48/207 (23.19%), Postives = 85/207 (41.06%), Query Frame = 3 
Query:  474 FDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGG-----------HDKF------FHKSGKK--------------TRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYG-----SPCKMGGYSHMV 986
              +RK++  LL  ++    ++N+TY +  G+LLG  R  D++PWD D+D+++      K++ I     P   F +  G           H+ F      F  +GK+               R      ++D  +Y E + ++  + +  G+  +Y K  VFPL    L+      PKD    L   Y  G       C+ G +  ++
Sbjct:   50 LQKRKLLVTLLRTWNLIASHHNITYSLMYGSLLGVERTADILPWDHDLDIMVSYDGILKLERIANICDPRGHFDINDGLPHLVVLPNSEHNVFMDDRRRFDCAGKEVAKLVDACSIQEPLARLIAGSVFMDFYHYTEKEDNLISVPE--GKTVTYAKGEVFPLKECFLMGVKSWCPKDPIRVLETWYGKGRLGSHKSCQSGEWVELI 254
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Match: EDO45603 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSB6])

HSP 1 Score: 52.373 bits (124), Expect = 1.279e-7
Identity = 20/52 (38.46%), Postives = 36/52 (69.23%), Query Frame = 3 
Query:  483 RKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVK 638
            RK +  +L+ + +    +N+TY +  G+L+G+ R +D+IPWD D+DL++P K
Sbjct:  117 RKPLTAILQYWTEITAKHNITYALMWGSLIGAIRTNDVIPWDHDMDLVVPYK 168
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Match: EDO45781 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRL8])

HSP 1 Score: 50.447 bits (119), Expect = 3.255e-7
Identity = 22/48 (45.83%), Postives = 28/48 (58.33%), Query Frame = 3 
Query:  486 KMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLII 629
            K +Y++    DQ    + + Y   GGT LG  RH  LIPWDDD+DL I
Sbjct:   22 KTLYQMAGDLDQLFTEHQINYWTDGGTTLGCVRHKGLIPWDDDIDLCI 69
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Medaka
Match: fkrp (fukutin related protein [Source:NCBI gene;Acc:101164830])

HSP 1 Score: 48.521 bits (114), Expect = 9.543e-6
Identity = 39/146 (26.71%), Postives = 65/146 (44.52%), Query Frame = 3 
Query:  525 MKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWP---YLDL-SYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYG 950
            ++++ V Y + GG+LLG+ RH D+IPWD DVDL I ++       +  K     + +   G+       G   R  +S     ++DL  +Y+ N     D    + Q   +P+  + PL   P    +   P +  AFL   +  G
Sbjct:  369 LESSGVRYWLEGGSLLGAVRHQDIIPWDYDVDLGIYLEDVPNCDHL--KNLDSGSLVDANGYVWERAVEGDFYRVQYSEANHLHVDLWPFYSRNGVMTKDTWTDHKQDVEFPEHFLQPLVPMPFAGITAFGPNNHKAFLELKFGEG 512
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000065543.1 (SMESG000065543.1)

HSP 1 Score: 738.413 bits (1905), Expect = 0.000e+0
Identity = 371/378 (98.15%), Postives = 373/378 (98.68%), Query Frame = 3 
Query:   81 ISNTFRMNNGKWFLLLVTFCVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKMVNSNKQNEKNKTHDVNFVKKXXXXXXXXXXXXXXRNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPPAK 1214
            +SN FR NNGKWFLLLVTFCV V IIIKV EQFSQTSFLQ+DRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKMVNSNKQNEKNKTHDVNFVKKLFENESFSNESLEERNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPPAK
Sbjct:    1 MSNLFRKNNGKWFLLLVTFCVSVTIIIKVKEQFSQTSFLQYDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKMVNSNKQNEKNKTHDVNFVKKLFENESFSNESLEERNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPPAK 378
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000065540.1 (SMESG000065540.1)

HSP 1 Score: 736.873 bits (1901), Expect = 0.000e+0
Identity = 371/372 (99.73%), Postives = 371/372 (99.73%), Query Frame = 3 
Query:   99 MNNGKWFLLLVTFCVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKMVNSNKQNEKNKTHDVNFVKKXXXXXXXXXXXXXXRNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPPAK 1214
            MNNGKWFLLLVTFCVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKMVNSNKQNEKNKTHDVNFVKKLFENESFSNESLEERNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQR KMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPPAK
Sbjct:    1 MNNGKWFLLLVTFCVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILASNLTKMVNSNKQNEKNKTHDVNFVKKLFENESFSNESLEERNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRVKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPPAK 372
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000065555.1 (SMESG000065555.1)

HSP 1 Score: 370.548 bits (950), Expect = 1.563e-126
Identity = 204/377 (54.11%), Postives = 264/377 (70.03%), Query Frame = 3 
Query:  120 LLLVTFCVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLISADERRALVQQIVEDSLREILA--SNLTKMVNSNK-----------QNEKNKTHDVNFVKKXXXXXXXXXXXXXXRNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTR-FSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPP 1208
            + +  F     +II+ N +F  +   QF  L+ L+L + +KL+   E+ AL++QIV DSL+ IL    N    V+  K           +N+  K  + N ++++F +    + +   RNS++K FQ+Q+T +ER  +Y LLE+FD+TMK +N+TY MYGGTLLGSFRHHDLIPWDDD+D+++       M+ IF+   P++ F   GGHDKF+HKS KK   +SW WPYLD+SYY  N T I D V+TY  WY +PKEIVFPLHNRPLLN  LP+P+DTFAFL W+YNYGS C M GYSHMVEGGGPSG  EC +++DKFPFVEHLPH +GVRE+LKYKD+EITDIV+PEPKYAVSETF LSLK GF MP +I PP
Sbjct:    1 MAITIFITTGYMIIQKNIEFHNS---QFSGLTNLQLLKLNKLLLPHEQAALIRQIVNDSLQTILQMQKNTISTVSPIKPIFDISTDDFGKNKYKKKLNSNLLQRVFHHAPALSGNHSVRNSELKAFQVQLTLEERSKMYELLEVFDRTMKEHNITYFMYGGTLLGSFRHHDLIPWDDDLDVMLSADNHKDMEMIFKPLEPDFKFYRAGGHDKFYHKSAKKASGYSWLWPYLDISYYEVNGTFIKDWVKTYSYWYYFPKEIVFPLHNRPLLNLLLPAPRDTFAFLTWSYNYGSECIMYGYSHMVEGGGPSGRTECSSLADKFPFVEHLPHADGVREVLKYKDLEITDIVLPEPKYAVSETFKLSLKSGFKMPSHITPP 374
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000079138.1 (SMESG000079138.1)

HSP 1 Score: 224.942 bits (572), Expect = 2.230e-70
Identity = 106/263 (40.30%), Postives = 160/263 (60.84%), Query Frame = 3 
Query:  435 RNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSP-EYTFIVVGGHDKFF--HKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGG-GPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPPA 1211
            R   MK F  Q++  ER+  Y+L+ LFD+ MK + + Y + GGTLLGS+R+HD +PWDDD+D+ + V  R +++++F   +           HDK +  ++S    +F W WPY+D+S+Y+ N THITDI +TYG++ + PK I+FPL  RP   + LPSP+D FA   W + +   C M  YSH++E G    G  +C    +++P+V    + +GV E L YK+  I  I++ EP YA+SE+F + LK GF  P +I  PA
Sbjct:   64 RMKTMKAFNYQISKTERQTFYKLISLFDRIMKQHGIPYFISGGTLLGSYRNHDFLPWDDDIDVYVNVGSRRQVEKVFTSLNRINVVLYYTTKHDKLYFDNESFFSNKFGWKWPYVDISFYDSNATHITDIAKTYGRYETVPKSIIFPLSTRPFFIFDLPSPRDAFANCAWMFGFTDMCSMHSYSHIIEAGYSGYGKEKCILFKNRYPYVNRYLNSDGVIEELVYKNTVIQRIIVNEPNYAISESFSIKLKYGFRFPSDIDLPA 326
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000017956.1 (SMESG000017956.1)

HSP 1 Score: 222.631 bits (566), Expect = 5.112e-70
Identity = 108/266 (40.60%), Postives = 165/266 (62.03%), Query Frame = 3 
Query:  435 RNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIF-EKFSPEYTFIVVGGHDK--FFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYPKEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEG-GGPSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVSETFMLSLKPGFSMPKNIMPPAK*H 1220
            R   + PF+ Q+T  ER  +YRLL +FD+TMK N + + ++ GTLLGS+R+HD IPWDDD+D+ + V +R +++++F  K            HDK  F + S   +++ W WPY+D+S+YN N TH+ D+  TYGQ+ +YPK I+FPL NRPL   +LPSPKD +A+ VW+Y +   C M  YSH+ E        ++CK  ++K+P+V+    +N V E L +K   I  I + EP YA+S++F   L+ G+  P ++  P + H
Sbjct:   10 RIGNIPPFEYQITKQERMTMYRLLAVFDKTMKENGIPFFIHSGTLLGSYRNHDFIPWDDDIDVFVSVAERSQLEKVFYNKNVSNLILYYTPTHDKIYFSNDSVSGSKYGWRWPYIDISFYNTNGTHLFDLSPTYGQYDTYPKSIIFPLTNRPLFTMNLPSPKDAYAYCVWSYGFSDDCVMNYYSHITENLMTKYVKVKCKVFANKYPYVKRYRTDNSVIEELLFKGRVIQRIKVNEPLYAISKSFGFVLQKGYPYPTDVSLPNQNH 275
The following BLAST results are available for this feature:
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8J1S01.508e-5639.92Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8G4T01.801e-5640.70Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1S8WS502.540e-5532.17Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A074ZZ743.571e-5532.17Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A3R7H1K89.480e-5332.15Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO445313.608e-932.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO355761.865e-842.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO455402.129e-823.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO456031.279e-738.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO457813.255e-745.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fkrp9.543e-626.71fukutin related protein [Source:NCBI gene;Acc:1011... [more]
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000065543.10.000e+098.15SMESG000065543.1[more]
SMESG000065540.10.000e+099.73SMESG000065540.1[more]
SMESG000065555.11.563e-12654.11SMESG000065555.1[more]
SMESG000079138.12.230e-7040.30SMESG000079138.1[more]
SMESG000017956.15.112e-7040.60SMESG000017956.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009860 ID=SMED30009860|Name=Lipopolysaccharide choline phosphotransferase protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1236bp
ATTTTGTTGACTTTATTTTTTTGCAATTATGTCAATAAATAAGAAACATT
TTAAAGAAATATTTTCAAGTTTTTTTATTTATATCGAATACATTTAGAAT
GAACAATGGAAAATGGTTTCTACTTTTAGTCACATTTTGTGTCGGTGTAA
TAATTATTATAAAGGTAAATGAACAATTCTCTCAGACTTCTTTTCTACAG
TTCGATCGTTTGTCAAGATTGAAACTCTCGGAATTTGATAAACTAATCTC
TGCAGATGAGCGTCGGGCATTAGTCCAGCAAATCGTTGAAGATTCTCTTA
GAGAGATTTTGGCATCCAACTTGACAAAAATGGTGAATTCCAACAAACAA
AATGAAAAGAACAAAACGCACGACGTAAACTTTGTTAAGAAACTTTTCGA
AAATGAAAGTTTCTCCAACGAAAGTCTTGAAGAAAGAAATTCTAAAATGA
AACCCTTTCAAATCCAAATGACGTTTGATGAAAGAAAAATGGTTTATAGG
TTATTGGAATTATTTGATCAAACAATGAAAAATAACAATGTGACATACAT
TATGTATGGTGGGACACTGTTGGGTTCATTCAGACATCATGATCTCATTC
CTTGGGATGATGATGTAGATCTTATTATACCAGTCAAACAAAGAGGAAAA
ATGAAAGAAATCTTTGAAAAGTTTTCTCCTGAGTATACTTTTATTGTAGT
AGGAGGGCATGATAAATTCTTCCATAAATCTGGAAAGAAAACTAGATTTT
CATGGTCATGGCCATATTTAGATTTAAGCTATTACAATGAAAACGATACG
CACATTACCGATATAGTACAAACGTACGGACAGTGGTATTCCTATCCCAA
AGAAATAGTATTTCCTTTACACAATAGACCTTTATTAAACTATTCATTGC
CTTCGCCTAAAGATACATTTGCATTTTTGGTTTGGACTTATAATTATGGA
TCTCCGTGTAAAATGGGCGGCTACTCTCACATGGTTGAAGGAGGTGGACC
GAGCGGGAATATCGAATGTAAAACAGTGAGCGACAAATTTCCTTTCGTAG
AACATTTGCCTCATGAAAATGGGGTTAGAGAGATTTTAAAATACAAAGAC
ATTGAAATAACAGATATAGTAATTCCTGAACCAAAATATGCAGTCTCGGA
AACATTTATGTTATCATTAAAACCCGGATTTAGTATGCCTAAAAATATTA
TGCCGCCAGCAAAATAACATATATTCAAAACCTGTG
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protein sequence of SMED30009860-orf-1

>SMED30009860-orf-1 ID=SMED30009860-orf-1|Name=SMED30009860-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=373bp
MNNGKWFLLLVTFCVGVIIIIKVNEQFSQTSFLQFDRLSRLKLSEFDKLI
SADERRALVQQIVEDSLREILASNLTKMVNSNKQNEKNKTHDVNFVKKLF
ENESFSNESLEERNSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTY
IMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIV
VGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQWYSYP
KEIVFPLHNRPLLNYSLPSPKDTFAFLVWTYNYGSPCKMGGYSHMVEGGG
PSGNIECKTVSDKFPFVEHLPHENGVREILKYKDIEITDIVIPEPKYAVS
ETFMLSLKPGFSMPKNIMPPAK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000419prepharyngeal region
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR007074LicD_fam
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007074LicD familyPFAMPF04991LicDcoord: 144..196
e-value: 1.5E-14
score: 54.7
NoneNo IPR availablePANTHERPTHR43404FAMILY NOT NAMEDcoord: 125..284
NoneNo IPR availableTMHMMTMhelixcoord: 7..29