Huntingtin-interacting protein K

Overview
NameHuntingtin-interacting protein K
Smed IDSMED30009845
Length (bp)950
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Huntingtin-interacting protein K (SMED30009845) t-SNE clustered cells

Violin plots show distribution of expression levels for Huntingtin-interacting protein K (SMED30009845) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Huntingtin-interacting protein K (SMED30009845) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Huntingtin-interacting protein K (SMED30009845) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009845

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30009845h1SMcG0010032 SmedASXL_013590SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30009845h1SMcG0010032 SmedASXL_013590SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30009845h1SMcG0010032 dd_Smed_v4_1948_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30009845h1SMcG0010032 dd_Smed_v4_1948_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30009845h1SMcG0010032 dd_Smed_v4_1948_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30009845h1SMcG0010032 dd_Smed_v4_1948_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009845h1SMcG0010032 dd_Smed_v4_1948_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Human
Match: HYPK (huntingtin interacting protein K [Source:HGNC Symbol;Acc:HGNC:18418])

HSP 1 Score: 58.5362 bits (140), Expect = 3.240e-10
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:   83 KKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   31 EKPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEA 123
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Human
Match: HYPK (huntingtin interacting protein K [Source:HGNC Symbol;Acc:HGNC:18418])

HSP 1 Score: 58.5362 bits (140), Expect = 3.240e-10
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:   83 KKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   31 EKPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEA 123
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Fly
Match: CG9922 (gene:FBgn0038196 transcript:FBtr0334297)

HSP 1 Score: 57.7658 bits (138), Expect = 2.741e-10
Identity = 36/90 (40.00%), Postives = 57/90 (63.33%), Query Frame = 2 
Query:   95 KHDELGAADLEKVTDFHEEVELTG-GVANIIQNIKS---------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +HD+ GAADLE+VTD+ EE E++   +++ ++   +         V K+ +L K++VK EDI LI+NE+ VS  +AE  L+E   DV+ A
Sbjct:   29 RHDD-GAADLERVTDYAEEKEISADNISSAVEQFGNQRNKENELRVAKEKELQKVQVKKEDIELIMNELLVSKAHAEKVLREQSGDVVAA 117
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Fly
Match: CG9922 (gene:FBgn0038196 transcript:FBtr0082870)

HSP 1 Score: 57.7658 bits (138), Expect = 2.741e-10
Identity = 36/90 (40.00%), Postives = 57/90 (63.33%), Query Frame = 2 
Query:   95 KHDELGAADLEKVTDFHEEVELTG-GVANIIQNIKS---------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +HD+ GAADLE+VTD+ EE E++   +++ ++   +         V K+ +L K++VK EDI LI+NE+ VS  +AE  L+E   DV+ A
Sbjct:   29 RHDD-GAADLERVTDYAEEKEISADNISSAVEQFGNQRNKENELRVAKEKELQKVQVKKEDIELIMNELLVSKAHAEKVLREQSGDVVAA 117
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Zebrafish
Match: hypk (huntingtin interacting protein K [Source:ZFIN;Acc:ZDB-GENE-080515-6])

HSP 1 Score: 60.8474 bits (146), Expect = 3.095e-11
Identity = 37/95 (38.95%), Postives = 60/95 (63.16%), Query Frame = 2 
Query:   80 SKKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            ++KP KHD  GAADLEKVTD+ EE E++        ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   22 TEKPRKHDS-GAADLEKVTDYAEEKEISSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDVELIMGEMEISRAVAERSLREHMGNVVEA 115
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Xenopus
Match: tfrc (transferrin receptor [Source:Xenbase;Acc:XB-GENE-487708])

HSP 1 Score: 59.3066 bits (142), Expect = 1.355e-10
Identity = 36/93 (38.71%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   86 KPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+NEM++    AE  L+E+  +V+ A
Sbjct:   26 KPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDVELIMNEMEIPRTAAERSLREHMGNVVEA 117
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Xenopus
Match: tfrc (transferrin receptor [Source:Xenbase;Acc:XB-GENE-487708])

HSP 1 Score: 58.9214 bits (141), Expect = 2.127e-10
Identity = 36/93 (38.71%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   86 KPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+NEM++    AE  L+E+  +V+ A
Sbjct:   25 KPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDVELIMNEMEIPRTAAERSLREHMGNVVEA 116
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Mouse
Match: Hypk (huntingtin interacting protein K [Source:MGI Symbol;Acc:MGI:1914943])

HSP 1 Score: 58.9214 bits (141), Expect = 1.792e-10
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:   83 KKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   31 EKPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEA 123
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Mouse
Match: Hypk (huntingtin interacting protein K [Source:MGI Symbol;Acc:MGI:1914943])

HSP 1 Score: 58.9214 bits (141), Expect = 1.792e-10
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:   83 KKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   31 EKPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEA 123
[Close]
BLAST of Huntingtin-interacting protein K vs. UniProt/SwissProt
Match: sp|Q9CR41|HYPK_MOUSE (Huntingtin-interacting protein K OS=Mus musculus OX=10090 GN=Hypk PE=1 SV=2)

HSP 1 Score: 58.9214 bits (141), Expect = 1.253e-9
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:   83 KKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   31 EKPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEA 123
[Close]
BLAST of Huntingtin-interacting protein K vs. UniProt/SwissProt
Match: sp|Q2PFU1|HYPK_MACFA (Huntingtin-interacting protein K OS=Macaca fascicularis OX=9541 GN=HYPK PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.556e-9
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:   83 KKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   31 EKPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEA 123
[Close]
BLAST of Huntingtin-interacting protein K vs. UniProt/SwissProt
Match: sp|Q9NX55|HYPK_HUMAN (Huntingtin-interacting protein K OS=Homo sapiens OX=9606 GN=HYPK PE=1 SV=2)

HSP 1 Score: 58.5362 bits (140), Expect = 1.556e-9
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:   83 KKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +KP KHD  GAADLE+VTD+ EE E+         ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   31 EKPRKHDS-GAADLERVTDYAEEKEIQSSNLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDLELIMTEMEISRAAAERSLREHMGNVVEA 123
[Close]
BLAST of Huntingtin-interacting protein K vs. TrEMBL
Match: A0A0R3U733 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS2624 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.603e-13
Identity = 42/93 (45.16%), Postives = 61/93 (65.59%), Query Frame = 2 
Query:   77 KSKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQNIKSVKKDNQL-----AKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPAPS 340
            KSKKPT HDE  AADLEK+TD  EE E++ G+ + ++ +   +K + +      +IK+KAED+++IV E ++S   AE KLKE  SD+  A S
Sbjct:   11 KSKKPTTHDEKNAADLEKMTDVFEETEVSAGMTDALKKVTGGEKQSSVPTERAPRIKIKAEDVDIIVQEFEISRAAAEKKLKEFHSDLRQALS 103
[Close]
BLAST of Huntingtin-interacting protein K vs. TrEMBL
Match: A0A074ZQX0 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_14165 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.951e-13
Identity = 45/92 (48.91%), Postives = 58/92 (63.04%), Query Frame = 2 
Query:   77 KSKKPTKHDELGAADLEKVTDFHEEVELTGG------VANIIQN---IKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDV 325
            K KKP  HDE GAADLE+VTD+ EE EL+ G      V+  + +       +K+ QL K+KVKAED+ LI  E++VS   AE +LKEN  DV
Sbjct:   11 KPKKPHTHDERGAADLERVTDYVEEKELSVGPDALTAVSGTVGDEGAASKAQKEKQLLKVKVKAEDVELICQELEVSKSVAERRLKENQGDV 102
[Close]
BLAST of Huntingtin-interacting protein K vs. TrEMBL
Match: A0A4S2MD66 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002191 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.053e-13
Identity = 45/92 (48.91%), Postives = 58/92 (63.04%), Query Frame = 2 
Query:   77 KSKKPTKHDELGAADLEKVTDFHEEVELTGG------VANIIQN---IKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDV 325
            K KKP  HDE GAADLE+VTD+ EE EL+ G      V+  + +       +K+ QL K+KVKAED+ LI  E++VS   AE +LKEN  DV
Sbjct:   11 KPKKPHTHDERGAADLERVTDYVEEKELSVGPDALTAVSGTVGDEGAASKAQKEKQLLKVKVKAEDVELICQELEVSKSVAERRLKENQGDV 102
[Close]
BLAST of Huntingtin-interacting protein K vs. TrEMBL
Match: H2KNY5 (Huntingtin-interacting protein K OS=Clonorchis sinensis OX=79923 GN=Hypk PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.053e-13
Identity = 45/92 (48.91%), Postives = 58/92 (63.04%), Query Frame = 2 
Query:   77 KSKKPTKHDELGAADLEKVTDFHEEVELTGG------VANIIQN---IKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDV 325
            K KKP  HDE GAADLE+VTD+ EE EL+ G      V+  + +       +K+ QL K+KVKAED+ LI  E++VS   AE +LKEN  DV
Sbjct:   11 KPKKPHTHDERGAADLERVTDYVEEKELSVGPDALTAVSGTVGDEGAASKAQKEKQLLKVKVKAEDVELICQELEVSKSVAERRLKENQGDV 102
[Close]
BLAST of Huntingtin-interacting protein K vs. TrEMBL
Match: G4VQH5 (Huntingtin interacting protein-related OS=Schistosoma mansoni OX=6183 GN=Smp_054560 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.075e-12
Identity = 43/90 (47.78%), Postives = 57/90 (63.33%), Query Frame = 2 
Query:   77 KSKKPTKHDELGAADLEKVTDFHEEVELTGG------VANIIQNIKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVM 328
            K KKPT HDE GAADLE+VTD+ EE EL+ G      V++   +    +K  Q+ KIKVK ED++LI  E++VS   AE  LKE+  +V 
Sbjct:   10 KPKKPTTHDERGAADLERVTDYFEEKELSVGLDAVKAVSDTAGDADKAQKQKQMLKIKVKNEDVSLICQELEVSKLTAERYLKEHQGNVF 99
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Cavefish
Match: hypk (huntingtin interacting protein K [Source:NCBI gene;Acc:103022837])

HSP 1 Score: 60.077 bits (144), Expect = 5.397e-11
Identity = 36/95 (37.89%), Postives = 60/95 (63.16%), Query Frame = 2 
Query:   80 SKKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            ++KP KHD  GAADLE+VTD+ EE E++        ++I + +S       +++ +LAK+ +K ED+ LI+ EM++S   AE  L+E+  +V+ A
Sbjct:   22 TEKPRKHDS-GAADLERVTDYAEEKEISSSDLETAMSVIGDRRSREQKAKQEREKELAKVTIKKEDVELIMGEMEISRGVAERSLREHMGNVVEA 115
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Sea Lamprey
Match: hypk (huntingtin interacting protein K [Source:ZFIN;Acc:ZDB-GENE-080515-6])

HSP 1 Score: 61.6178 bits (148), Expect = 3.243e-12
Identity = 37/98 (37.76%), Postives = 62/98 (63.27%), Query Frame = 2 
Query:   80 SKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQNIKSV-------------KKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            ++KP KHD  GAADLEKVTD+ E+ E++   A+ +++  SV             +++N+LAK+ ++ ED+ LI+NEM++    AE  L+E+  +V+ A
Sbjct:   32 AEKPRKHDS-GAADLEKVTDYAEDKEIS---ASSLESAMSVIGDRRSKEQTAKQQRENELAKVTIRKEDVELIMNEMEIGRTAAERSLREHMGNVVEA 125
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Nematostella
Match: EDO46378 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQK2])

HSP 1 Score: 56.9954 bits (136), Expect = 1.406e-10
Identity = 29/89 (32.58%), Postives = 53/89 (59.55%), Query Frame = 2 
Query:   95 KHDELGAADLEKVTDFHEEVELTGGVANIIQNIKS---------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            +HD   +ADLE+VTD+ EE E+T  +   +  ++           +++ +LA++ +K ED++LIV+EM++    AE  L+E+   V+ A
Sbjct:   13 RHDLQTSADLERVTDYAEETEITADIGTALSLVEGRHLKEKADQAQREKELARVVIKKEDVDLIVDEMEIQRPLAERTLREHKGSVVDA 101
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Medaka
Match: hypk (huntingtin interacting protein K [Source:NCBI gene;Acc:101169905])

HSP 1 Score: 58.9214 bits (141), Expect = 1.229e-10
Identity = 36/95 (37.89%), Postives = 60/95 (63.16%), Query Frame = 2 
Query:   80 SKKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPA 334
            ++KP KHD  GAADLE+VTD+ EE E++        ++I + +S       +++ +LAK+ +K ED+ LI++EM++S   AE  L+E+  +V  A
Sbjct:   22 AEKPRKHDS-GAADLERVTDYAEEKEISSSDLQTAMSVIGDRRSREQKAKQEREKELAKVTIKKEDVELIMSEMEISRAVAERSLREHLGNVGKA 115
[Close]
BLAST of Huntingtin-interacting protein K vs. Ensembl Medaka
Match: hypk (huntingtin interacting protein K [Source:NCBI gene;Acc:101169905])

HSP 1 Score: 46.9802 bits (110), Expect = 1.627e-6
Identity = 28/72 (38.89%), Postives = 46/72 (63.89%), Query Frame = 2 
Query:   80 SKKPTKHDELGAADLEKVTDFHEEVELTGG----VANIIQNIKS------VKKDNQLAKIKVKAEDINLIVN 265
            ++KP KHD  GAADLE+VTD+ EE E++        ++I + +S       +++ +LAK+ +K ED+ LIV+
Sbjct:   22 AEKPRKHDS-GAADLERVTDYAEEKEISSSDLQTAMSVIGDRRSREQKAKQEREKELAKVTIKKEDVELIVS 92
[Close]
BLAST of Huntingtin-interacting protein K vs. Planmine SMEST
Match: SMESG000064372.1 (SMESG000064372.1)

HSP 1 Score: 437.958 bits (1125), Expect = 6.930e-157
Identity = 215/216 (99.54%), Postives = 216/216 (100.00%), Query Frame = 2 
Query:   77 KSKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQNIKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPAPSKRIKLNNYEYSVNNNATSDNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMVKNQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIADRDHVRADDAIKLPIIKSGESGSANAISR 724
            KSKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQNIKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPAPSKRIKLNNYEYSVNNNATSDNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMV+NQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIADRDHVRADDAIKLPIIKSGESGSANAISR
Sbjct:   13 KSKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQNIKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPAPSKRIKLNNYEYSVNNNATSDNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMVENQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIADRDHVRADDAIKLPIIKSGESGSANAISR 228
[Close]
BLAST of Huntingtin-interacting protein K vs. Planmine SMEST
Match: SMESG000064372.1 (SMESG000064372.1)

HSP 1 Score: 381.719 bits (979), Expect = 5.014e-135
Identity = 193/216 (89.35%), Postives = 194/216 (89.81%), Query Frame = 2 
Query:   77 KSKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQNIKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPAPSKRIKLNNYEYSVNNNATSDNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMVKNQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIADRDHVRADDAIKLPIIKSGESGSANAISR 724
            KSKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQNIKSVKKDNQLAKIKVKAEDINLI                      MPAPSKRIKLNNYEYSVNNNATSDNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMV+NQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIADRDHVRADDAIKLPIIKSGESGSANAISR
Sbjct:   13 KSKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQNIKSVKKDNQLAKIKVKAEDINLI----------------------MPAPSKRIKLNNYEYSVNNNATSDNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMVENQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIADRDHVRADDAIKLPIIKSGESGSANAISR 206
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BLAST of Huntingtin-interacting protein K vs. Planmine SMEST
Match: SMESG000041408.1 (SMESG000041408.1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.218e-11
Identity = 35/101 (34.65%), Postives = 60/101 (59.41%), Query Frame = 2 
Query:  395 DNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMVKNQAQQFLITEQRRVLDRIYTEDELRRRIP------NIDIADRDHVRADDAIKLP 679
            + L  +   ++A+ +KLK TW +P    K+ QK  +LT++  EL++++++Q+ LI EQ   L RI+TE ELR R+P      ++DI    H+ ++    LP
Sbjct:   25 EQLEKDHTVLQAKYQKLKGTWSSPESTKKLFQKIARLTRARDELIIESRSQRILIAEQSTALRRIFTEAELRARLPGCSLAQSLDIIPAQHLPSNPTPSLP 125
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BLAST of Huntingtin-interacting protein K vs. Planmine SMEST
Match: SMESG000068149.1 (SMESG000068149.1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.124e-9
Identity = 27/81 (33.33%), Postives = 52/81 (64.20%), Query Frame = 2 
Query:  395 DNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMVKNQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIA 637
            D L  + A ++A+ +KLK TW +P     + Q+  +L+++  +L+++ + Q+  I EQ   L RI+TE+E+R R+P+ ++A
Sbjct:   45 DQLERDHAILRAKYEKLKGTWSSPETTSNLYQRISRLSRARDDLIIETRGQRITIAEQSAALTRIFTENEVRTRLPSCNLA 125
[Close]
BLAST of Huntingtin-interacting protein K vs. Planmine SMEST
Match: SMESG000071773.1 (SMESG000071773.1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.422e-9
Identity = 29/81 (35.80%), Postives = 51/81 (62.96%), Query Frame = 2 
Query:  395 DNLTNESAYVKAELKKLKATWYNPLRVIKMKQKEFKLTQSCAELMVKNQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIA 637
            + L  +   ++A+ +KLK TW +P    K+ QK  +L+++  +L+V++++Q+ LI +Q   L RI+TE ELR R+P    A
Sbjct:   22 EQLQKDHTVLQAKYQKLKGTWSSPEATRKLFQKSSRLSRARDDLVVESRSQRILIAKQSAALRRIFTEAELRARLPGCSWA 102
The following BLAST results are available for this feature:
BLAST of Huntingtin-interacting protein K vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
HYPK3.240e-1038.30huntingtin interacting protein K [Source:HGNC Symb... [more]
HYPK3.240e-1038.30huntingtin interacting protein K [Source:HGNC Symb... [more]
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BLAST of Huntingtin-interacting protein K vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Huntingtin-interacting protein K vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG99222.741e-1040.00gene:FBgn0038196 transcript:FBtr0334297[more]
CG99222.741e-1040.00gene:FBgn0038196 transcript:FBtr0082870[more]
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BLAST of Huntingtin-interacting protein K vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hypk3.095e-1138.95huntingtin interacting protein K [Source:ZFIN;Acc:... [more]
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BLAST of Huntingtin-interacting protein K vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tfrc1.355e-1038.71transferrin receptor [Source:Xenbase;Acc:XB-GENE-4... [more]
tfrc2.127e-1038.71transferrin receptor [Source:Xenbase;Acc:XB-GENE-4... [more]
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BLAST of Huntingtin-interacting protein K vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Hypk1.792e-1038.30huntingtin interacting protein K [Source:MGI Symbo... [more]
Hypk1.792e-1038.30huntingtin interacting protein K [Source:MGI Symbo... [more]
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BLAST of Huntingtin-interacting protein K vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q9CR41|HYPK_MOUSE1.253e-938.30Huntingtin-interacting protein K OS=Mus musculus O... [more]
sp|Q2PFU1|HYPK_MACFA1.556e-938.30Huntingtin-interacting protein K OS=Macaca fascicu... [more]
sp|Q9NX55|HYPK_HUMAN1.556e-938.30Huntingtin-interacting protein K OS=Homo sapiens O... [more]
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BLAST of Huntingtin-interacting protein K vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3U7331.603e-1345.16Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A074ZQX01.951e-1348.91Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A4S2MD662.053e-1348.91Uncharacterized protein OS=Opisthorchis felineus O... [more]
H2KNY52.053e-1348.91Huntingtin-interacting protein K OS=Clonorchis sin... [more]
G4VQH51.075e-1247.78Huntingtin interacting protein-related OS=Schistos... [more]
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BLAST of Huntingtin-interacting protein K vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hypk5.397e-1137.89huntingtin interacting protein K [Source:NCBI gene... [more]
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BLAST of Huntingtin-interacting protein K vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hypk3.243e-1237.76huntingtin interacting protein K [Source:ZFIN;Acc:... [more]
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BLAST of Huntingtin-interacting protein K vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Huntingtin-interacting protein K vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO463781.406e-1032.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Huntingtin-interacting protein K vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hypk1.229e-1037.89huntingtin interacting protein K [Source:NCBI gene... [more]
hypk1.627e-638.89huntingtin interacting protein K [Source:NCBI gene... [more]
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BLAST of Huntingtin-interacting protein K vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000064372.16.930e-15799.54SMESG000064372.1[more]
SMESG000064372.15.014e-13589.35SMESG000064372.1[more]
SMESG000041408.11.218e-1134.65SMESG000041408.1[more]
SMESG000068149.12.124e-933.33SMESG000068149.1[more]
SMESG000071773.12.422e-935.80SMESG000071773.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009845 ID=SMED30009845|Name=Huntingtin-interacting protein K|organism=Schmidtea mediterranea sexual|type=transcript|length=950bp
TCTTTGTTTTAAATATGACATAATTTAAATAGAATACATCATGGACTATA
CTGAAGAAGTAGAAGATGAAGATGTTAAATCTAAAAAACCTACTAAGCAC
GATGAATTAGGTGCAGCAGATCTCGAAAAAGTTACAGATTTTCACGAAGA
AGTCGAATTAACTGGAGGAGTTGCTAATATTATTCAAAATATTAAATCTG
TAAAAAAAGATAATCAATTAGCCAAAATAAAAGTTAAGGCAGAAGATATT
AATTTAATAGTAAATGAAATGGATGTCTCAAGTAAATATGCCGAAGGAAA
ATTAAAAGAAAATGATAGCGACGTGATGCCAGCCCCCTCGAAGCGAATCA
AACTCAACAATTACGAATACTCAGTAAACAACAATGCAACTTCCGACAAC
CTAACGAATGAATCAGCATATGTAAAAGCTGAACTAAAAAAACTAAAGGC
GACCTGGTACAATCCTCTTCGGGTTATTAAAATGAAACAGAAAGAATTTA
AATTAACCCAATCTTGTGCCGAATTGATGGTCAAAAATCAGGCTCAACAG
TTCTTGATTACTGAACAAAGAAGAGTTCTCGACAGAATCTACACAGAAGA
TGAACTAAGACGTCGAATACCAAATATAGACATAGCCGATCGGGATCATG
TACGGGCTGATGACGCTATTAAGTTGCCAATCATAAAATCCGGAGAATCA
GGCTCCGCCAATGCCATATCTAGGTAACCTGACATCCGTCAAATATCTTA
TTTTCGCCCTAACTAAACGAATATCTGAACAAGAATATGATCTGGCTTAT
CTTAATGACATTTTATTCCCTTTCGGAACTTTAATAACCATGATTTTGTG
TGCATCTGTCACTTAGGTTAAATTGAATTGTTTTCTAGTATAATATTTTC
ATCTTTTATTTGCTTTGTTTATAATTATCGATTTATTTTTATGCTCGGCT
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protein sequence of SMED30009845-orf-1

>SMED30009845-orf-1 ID=SMED30009845-orf-1|Name=SMED30009845-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=229bp
MDYTEEVEDEDVKSKKPTKHDELGAADLEKVTDFHEEVELTGGVANIIQN
IKSVKKDNQLAKIKVKAEDINLIVNEMDVSSKYAEGKLKENDSDVMPAPS
KRIKLNNYEYSVNNNATSDNLTNESAYVKAELKKLKATWYNPLRVIKMKQ
KEFKLTQSCAELMVKNQAQQFLITEQRRVLDRIYTEDELRRRIPNIDIAD
RDHVRADDAIKLPIIKSGESGSANAISR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR038922HYPK_UBA
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005737cytoplasm
GO:0015630microtubule cytoskeleton
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
IPR038922Huntingtin-interacting protein K, UBA-like domainCDDcd14361UBA_HYPKcoord: 63..95
e-value: 3.05998E-7
score: 43.6511