Diacylglycerol kinase

Overview
NameDiacylglycerol kinase
Smed IDSMED30009837
Length (bp)3344
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Diacylglycerol kinase (SMED30009837) t-SNE clustered cells

Violin plots show distribution of expression levels for Diacylglycerol kinase (SMED30009837) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Diacylglycerol kinase (SMED30009837) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Diacylglycerol kinase (SMED30009837) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30009837

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30009837h1SMcG0013879 dd_Smed_v4_6249_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30009837h1SMcG0013879 dd_Smed_v4_6249_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30009837h1SMcG0013879 dd_Smed_v4_6249_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30009837h1SMcG0013879 dd_Smed_v4_6249_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30009837h1SMcG0013879 dd_Smed_v4_6249_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009837h1SMcG0013879 Contig38827newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009837h1SMcG0013879 Contig38827uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009837h1SMcG0013879 Contig29238newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009837h1SMcG0013879 Contig29238uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Diacylglycerol kinase vs. Ensembl Human
Match: DGKB (diacylglycerol kinase beta [Source:HGNC Symbol;Acc:HGNC:2850])

HSP 1 Score: 502.286 bits (1292), Expect = 1.061e-162
Identity = 252/455 (55.38%), Postives = 312/455 (68.57%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E IISQMM+VA YL WD  EL PIL +MM EIDYD+DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+K++  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL+   T+     +++ +  +S    + +  +   +  +            Q +NS   +  QGL        Q+  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  133 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTLPTSG----VSVPEERQSTVKKEKSGSQQPNKVIDKNKM--------QRANS-VTVDGQGL--------QVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKF 564          

HSP 2 Score: 229.565 bits (584), Expect = 2.035e-62
Identity = 118/212 (55.66%), Postives = 146/212 (68.87%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTIS 3096
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KK+    R E +   + +T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CT+S
Sbjct:  576 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTVS 771          
BLAST of Diacylglycerol kinase vs. Ensembl Human
Match: DGKB (diacylglycerol kinase beta [Source:HGNC Symbol;Acc:HGNC:2850])

HSP 1 Score: 502.286 bits (1292), Expect = 2.924e-162
Identity = 252/455 (55.38%), Postives = 312/455 (68.57%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E IISQMM+VA YL WD  EL PIL +MM EIDYD+DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+K++  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL+   T+     +++ +  +S    + +  +   +  +            Q +NS   +  QGL        Q+  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  133 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTLPTSG----VSVPEERQSTVKKEKSGSQQPNKVIDKNKM--------QRANS-VTVDGQGL--------QVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKF 564          

HSP 2 Score: 253.447 bits (646), Expect = 2.447e-70
Identity = 129/228 (56.58%), Postives = 158/228 (69.30%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KK+    R E +   + +T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+M PP K
Sbjct:  576 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPK 787          
BLAST of Diacylglycerol kinase vs. Ensembl Human
Match: DGKB (diacylglycerol kinase beta [Source:HGNC Symbol;Acc:HGNC:2850])

HSP 1 Score: 502.286 bits (1292), Expect = 2.924e-162
Identity = 252/455 (55.38%), Postives = 312/455 (68.57%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E IISQMM+VA YL WD  EL PIL +MM EIDYD+DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+K++  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL+   T+     +++ +  +S    + +  +   +  +            Q +NS   +  QGL        Q+  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  133 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTLPTSG----VSVPEERQSTVKKEKSGSQQPNKVIDKNKM--------QRANS-VTVDGQGL--------QVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKF 564          

HSP 2 Score: 253.447 bits (646), Expect = 2.447e-70
Identity = 129/228 (56.58%), Postives = 158/228 (69.30%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KK+    R E +   + +T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+M PP K
Sbjct:  576 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPK 787          
BLAST of Diacylglycerol kinase vs. Ensembl Human
Match: DGKB (diacylglycerol kinase beta [Source:HGNC Symbol;Acc:HGNC:2850])

HSP 1 Score: 499.975 bits (1286), Expect = 1.600e-161
Identity = 251/455 (55.16%), Postives = 310/455 (68.13%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E IISQMM+VA YL WD  EL PIL +MM EIDYD+DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+K++  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL    +      +++ +  +S    + +  +   +  +            Q +NS   +  QGL        Q+  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  133 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTLPTSG-----VSVPEERQSTVKKEKSGSQQPNKVIDKNKM--------QRANS-VTVDGQGL--------QVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKF 563          

HSP 2 Score: 253.062 bits (645), Expect = 2.502e-70
Identity = 129/228 (56.58%), Postives = 158/228 (69.30%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KK+    R E +   + +T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+M PP K
Sbjct:  575 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPK 786          
BLAST of Diacylglycerol kinase vs. Ensembl Human
Match: DGKB (diacylglycerol kinase beta [Source:HGNC Symbol;Acc:HGNC:2850])

HSP 1 Score: 499.975 bits (1286), Expect = 1.627e-161
Identity = 251/455 (55.16%), Postives = 310/455 (68.13%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E IISQMM+VA YL WD  EL PIL +MM EIDYD+DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+K++  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL    +      +++ +  +S    + +  +   +  +            Q +NS   +  QGL        Q+  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  126 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTLPTSG-----VSVPEERQSTVKKEKSGSQQPNKVIDKNKM--------QRANS-VTVDGQGL--------QVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKF 556          

HSP 2 Score: 253.062 bits (645), Expect = 2.708e-70
Identity = 129/228 (56.58%), Postives = 158/228 (69.30%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KK+    R E +   + +T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+M PP K
Sbjct:  568 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPK 779          

HSP 3 Score: 56.225 bits (134), Expect = 3.579e-7
Identity = 32/104 (30.77%), Postives = 51/104 (49.04%), Query Frame = 1
Query:   82 MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSK 393
            MT     +H +  +F+QLQ +   +   L+DV++ EF     LA+Y+PE  ID  G+  F+  +L    P      LF+SF  +          P+ +P +KSK
Sbjct:    1 MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLE-EFHGNGVLAKYNPEGTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKF---------PHSSPMVKSK 94          
BLAST of Diacylglycerol kinase vs. Ensembl Celegans
Match: dgk-3 (Probable diacylglycerol kinase 3 [Source:UniProtKB/Swiss-Prot;Acc:Q03603])

HSP 1 Score: 669.848 bits (1727), Expect = 0.000e+0
Identity = 352/758 (46.44%), Postives = 466/758 (61.48%), Query Frame = 1
Query:  877 TDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRF-GRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKK 3147
            + + +I +K L+C LSLLE+  PE+KL+ +F +YD+DGNG LDKSE++ II QMMNVA Y  WDT EL  +++QMM +IDYDNDG V+ +EW+RGG+TNIPLLVLLG DT +++DG HVW+L+HF+K T+C+ C  ILV + G+QGL C  C YTVHERCV++A  +CI TY+ +++ D+L  HHW + +  AKC+KCK +  +G   G  CRWC   +H  CM +  Q CDLG    HILPP  I+P+ LERK +   K   N   +S S +L  + +   DC                                              +PLLV +NPKSGGKQG +I+ KF+Y+LNPRQVYDL K  GP  GLQ F  +K+  IL+CGGDGT+GW+LE++DK  + + RP VA+LPLGTGNDLARCLRWGGGYE E L KIL +I   +++ +DRWQI                                 + ++ S++  S           E+GD                P +IINNYFSIGVDASIAH+FHV+REK PEKFNSRMRN+ +Y E+GTSE L+++CK+LHE IDI+CDG  +DL + A LEGIA+LNIPS+YGG NLWG                 + +   GR       + +    L T VQD+GD L+E+VGLE+AM  G +  G R + +RL+QC  VVI+T + FPMQIDGEPW+Q  C I IT+KNQ  M++A   +K
Sbjct:  149 SQEPRIPLKPLICTLSLLEADTPENKLDVVFHVYDSDGNGFLDKSEIDGIIEQMMNVARYQQWDTIELEQVIRQMMVDIDYDNDGIVSFDEWRRGGLTNIPLLVLLGFDTEMKEDGSHVWRLRHFTKPTYCNACCSILVGWGGKQGLSCSLCKYTVHERCVRSAATNCIRTYS-SRQQDKLY-HHWQDANATAKCVKCKAT--VGVFQGKGCRWCHNYVHHRCMSALAQECDLGALVHHILPPTHIFPAFLERKTSTSLK---NHNFSSHSASLLQAVSPSNDC----------------------------------------------RPLLVLVNPKSGGKQGVKILQKFEYLLNPRQVYDLSK-TGPEPGLQLFSTLKNCNILVCGGDGTIGWVLESMDKMTFPHGRPPVAVLPLGTGNDLARCLRWGGGYENENLHKILEQIEKSSLIDMDRWQIKIE------------------------------ITENKSARRAS-----------EKGDTP--------------PYSIINNYFSIGVDASIAHRFHVMREKFPEKFNSRMRNKLWYFELGTSETLSSSCKNLHEQIDILCDGESIDLGQDASLEGIALLNIPSIYGGSNLWG-----------------RSRKSKGRMPGLFPMKNAEKMQLQTRVQDIGDGLIELVGLESAMQMGQIKAGVRGA-RRLSQCSTVVIQTHKSFPMQIDGEPWMQPPCIIQITHKNQAKMLVAAAPRK 779          
BLAST of Diacylglycerol kinase vs. Ensembl Celegans
Match: dgk-5 (Putative diacylglycerol kinase K06A1.6 [Source:UniProtKB/Swiss-Prot;Acc:Q10024])

HSP 1 Score: 234.572 bits (597), Expect = 6.312e-64
Identity = 181/600 (30.17%), Postives = 263/600 (43.83%), Query Frame = 1
Query: 1390 TKTKKS-DQLLEHHWIEN-HYPAKCIKCKKSTKLGAKSGFH-----------CRWCKRTIH-KCCM--ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQ--ISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRD-VKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVM-LDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            T +K+S D L  HHW+    +  +C  C KS     K  F            C WCK + H K C   +   + C+ G  KE I+PP  I                         + LA+                        +SS +    ++   +H Q  +    + +   ++PLLVF+NPKSGG +G++ +    ++LNPRQV+D+   KGP  GL+ FR  V   RIL+CGGDGTVGW+L  +D   W    P +AI+PLGTGNDLARC+ WGG +  E + +++  I+  T+V  LDRW+I   EP                T C             +  + D G++                           +P  ++ NYFSIG DA +A +FH  R  +P+  NSR++NR  Y  +GT ++   + K L EYI + CDGV +  R K   L  I   NI    GG   WG                                 ES+ +N  +      D  +EV+G   A  A L  GG    G+R+AQC  V + T +  PMQ+DGEP + A   I++ + ++VPM+
Sbjct:  228 TPSKESTDGLTAHHWVHKWRHEGRCNTCAKS--FQQKMFFQGKEKKETIAVTCSWCKESYHLKNCFARDKLEERCNRGALKEMIVPPTWI-------------------------LRLANRK----------------------RSSRTPSHPRKHKKSHRQFVVKPTDLWSSGPSQPLLVFVNPKSGGNKGSKALHTLCWLLNPRQVFDITSLKGPKFGLEMFRKVVTQLRILVCGGDGTVGWVLSTLDNLNW-PAYPPMAIMPLGTGNDLARCMGWGGVFSDEPISQLMQAILHETIVTHLDRWRIDV-EP---------------NTSC-------------NLEEEDDGMQ-------------------------SALPLTVMTNYFSIGADAHVALQFHHSRSANPQMLNSRLKNRIAYGGLGTIDLFKRSWKDLCEYITLECDGVDVTPRIKELKLHCILFHNITYYAGGTIPWG---------------------------------ESS-DNKPSCC----DGKVEVLGFTTATLAALQMGG---KGERIAQCSRVRVITNKAIPMQVDGEPCLLAPSIITLGFHSKVPML 682          
BLAST of Diacylglycerol kinase vs. Ensembl Celegans
Match: dgk-5 (Putative diacylglycerol kinase K06A1.6 [Source:UniProtKB/Swiss-Prot;Acc:Q10024])

HSP 1 Score: 234.958 bits (598), Expect = 8.040e-64
Identity = 181/600 (30.17%), Postives = 263/600 (43.83%), Query Frame = 1
Query: 1390 TKTKKS-DQLLEHHWIEN-HYPAKCIKCKKSTKLGAKSGFH-----------CRWCKRTIH-KCCM--ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQ--ISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRD-VKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVM-LDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            T +K+S D L  HHW+    +  +C  C KS     K  F            C WCK + H K C   +   + C+ G  KE I+PP  I                         + LA+                        +SS +    ++   +H Q  +    + +   ++PLLVF+NPKSGG +G++ +    ++LNPRQV+D+   KGP  GL+ FR  V   RIL+CGGDGTVGW+L  +D   W    P +AI+PLGTGNDLARC+ WGG +  E + +++  I+  T+V  LDRW+I   EP                T C             +  + D G++                           +P  ++ NYFSIG DA +A +FH  R  +P+  NSR++NR  Y  +GT ++   + K L EYI + CDGV +  R K   L  I   NI    GG   WG                                 ES+ +N  +      D  +EV+G   A  A L  GG    G+R+AQC  V + T +  PMQ+DGEP + A   I++ + ++VPM+
Sbjct:  290 TPSKESTDGLTAHHWVHKWRHEGRCNTCAKS--FQQKMFFQGKEKKETIAVTCSWCKESYHLKNCFARDKLEERCNRGALKEMIVPPTWI-------------------------LRLANRK----------------------RSSRTPSHPRKHKKSHRQFVVKPTDLWSSGPSQPLLVFVNPKSGGNKGSKALHTLCWLLNPRQVFDITSLKGPKFGLEMFRKVVTQLRILVCGGDGTVGWVLSTLDNLNW-PAYPPMAIMPLGTGNDLARCMGWGGVFSDEPISQLMQAILHETIVTHLDRWRIDV-EP---------------NTSC-------------NLEEEDDGMQ-------------------------SALPLTVMTNYFSIGADAHVALQFHHSRSANPQMLNSRLKNRIAYGGLGTIDLFKRSWKDLCEYITLECDGVDVTPRIKELKLHCILFHNITYYAGGTIPWG---------------------------------ESS-DNKPSCC----DGKVEVLGFTTATLAALQMGG---KGERIAQCSRVRVITNKAIPMQVDGEPCLLAPSIITLGFHSKVPML 744          
BLAST of Diacylglycerol kinase vs. Ensembl Celegans
Match: dgk-5 (Putative diacylglycerol kinase K06A1.6 [Source:UniProtKB/Swiss-Prot;Acc:Q10024])

HSP 1 Score: 234.958 bits (598), Expect = 9.169e-64
Identity = 181/600 (30.17%), Postives = 263/600 (43.83%), Query Frame = 1
Query: 1390 TKTKKS-DQLLEHHWIEN-HYPAKCIKCKKSTKLGAKSGFH-----------CRWCKRTIH-KCCM--ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQ--ISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRD-VKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVM-LDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            T +K+S D L  HHW+    +  +C  C KS     K  F            C WCK + H K C   +   + C+ G  KE I+PP  I                         + LA+                        +SS +    ++   +H Q  +    + +   ++PLLVF+NPKSGG +G++ +    ++LNPRQV+D+   KGP  GL+ FR  V   RIL+CGGDGTVGW+L  +D   W    P +AI+PLGTGNDLARC+ WGG +  E + +++  I+  T+V  LDRW+I   EP                T C             +  + D G++                           +P  ++ NYFSIG DA +A +FH  R  +P+  NSR++NR  Y  +GT ++   + K L EYI + CDGV +  R K   L  I   NI    GG   WG                                 ES+ +N  +      D  +EV+G   A  A L  GG    G+R+AQC  V + T +  PMQ+DGEP + A   I++ + ++VPM+
Sbjct:  294 TPSKESTDGLTAHHWVHKWRHEGRCNTCAKS--FQQKMFFQGKEKKETIAVTCSWCKESYHLKNCFARDKLEERCNRGALKEMIVPPTWI-------------------------LRLANRK----------------------RSSRTPSHPRKHKKSHRQFVVKPTDLWSSGPSQPLLVFVNPKSGGNKGSKALHTLCWLLNPRQVFDITSLKGPKFGLEMFRKVVTQLRILVCGGDGTVGWVLSTLDNLNW-PAYPPMAIMPLGTGNDLARCMGWGGVFSDEPISQLMQAILHETIVTHLDRWRIDV-EP---------------NTSC-------------NLEEEDDGMQ-------------------------SALPLTVMTNYFSIGADAHVALQFHHSRSANPQMLNSRLKNRIAYGGLGTIDLFKRSWKDLCEYITLECDGVDVTPRIKELKLHCILFHNITYYAGGTIPWG---------------------------------ESS-DNKPSCC----DGKVEVLGFTTATLAALQMGG---KGERIAQCSRVRVITNKAIPMQVDGEPCLLAPSIITLGFHSKVPML 748          
BLAST of Diacylglycerol kinase vs. Ensembl Celegans
Match: dgk-5 (Putative diacylglycerol kinase K06A1.6 [Source:UniProtKB/Swiss-Prot;Acc:Q10024])

HSP 1 Score: 235.343 bits (599), Expect = 1.138e-63
Identity = 181/600 (30.17%), Postives = 263/600 (43.83%), Query Frame = 1
Query: 1390 TKTKKS-DQLLEHHWIEN-HYPAKCIKCKKSTKLGAKSGFH-----------CRWCKRTIH-KCCM--ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQ--ISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRD-VKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVM-LDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            T +K+S D L  HHW+    +  +C  C KS     K  F            C WCK + H K C   +   + C+ G  KE I+PP  I                         + LA+                        +SS +    ++   +H Q  +    + +   ++PLLVF+NPKSGG +G++ +    ++LNPRQV+D+   KGP  GL+ FR  V   RIL+CGGDGTVGW+L  +D   W    P +AI+PLGTGNDLARC+ WGG +  E + +++  I+  T+V  LDRW+I   EP                T C             +  + D G++                           +P  ++ NYFSIG DA +A +FH  R  +P+  NSR++NR  Y  +GT ++   + K L EYI + CDGV +  R K   L  I   NI    GG   WG                                 ES+ +N  +      D  +EV+G   A  A L  GG    G+R+AQC  V + T +  PMQ+DGEP + A   I++ + ++VPM+
Sbjct:  339 TPSKESTDGLTAHHWVHKWRHEGRCNTCAKS--FQQKMFFQGKEKKETIAVTCSWCKESYHLKNCFARDKLEERCNRGALKEMIVPPTWI-------------------------LRLANRK----------------------RSSRTPSHPRKHKKSHRQFVVKPTDLWSSGPSQPLLVFVNPKSGGNKGSKALHTLCWLLNPRQVFDITSLKGPKFGLEMFRKVVTQLRILVCGGDGTVGWVLSTLDNLNW-PAYPPMAIMPLGTGNDLARCMGWGGVFSDEPISQLMQAILHETIVTHLDRWRIDV-EP---------------NTSC-------------NLEEEDDGMQ-------------------------SALPLTVMTNYFSIGADAHVALQFHHSRSANPQMLNSRLKNRIAYGGLGTIDLFKRSWKDLCEYITLECDGVDVTPRIKELKLHCILFHNITYYAGGTIPWG---------------------------------ESS-DNKPSCC----DGKVEVLGFTTATLAALQMGG---KGERIAQCSRVRVITNKAIPMQVDGEPCLLAPSIITLGFHSKVPML 793          
BLAST of Diacylglycerol kinase vs. Ensembl Fly
Match: Dgk (gene:FBgn0085390 transcript:FBtr0112581)

HSP 1 Score: 488.419 bits (1256), Expect = 8.016e-154
Identity = 247/489 (50.51%), Postives = 319/489 (65.24%), Query Frame = 1
Query:  817 EKSALTLSKSNNLDIVNRPLTDQD----KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLD-TNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSD--QLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPD--TKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            E  +    K  ++ ++ R L+  D    K+ +KD++CYLSLLE+G+PEDKLEFMFRLYDTD NGVLD +E++ I++QMM VA YL WD  EL PILQ+MM EIDYD DG V+L+EW+RGGMT IPLLVLLG+D T +++DG HVW+LKHFSK  +C+LC  +LV  G++GLCCV C YTVHERCVQ+APASCI TY K+KK      L HHW+E +   +C KC+K  K     +G  CRWC   +H  C  S ++ C LG+  E I+PP  I P+VL+R                                               Q S +Q   + Q ++H+Q + FQI   PD  + PLLVF+NPKSGG+QG RI+ KFQY+LNPRQVYDL K  GP  GL  F+D+  FR++ CGGDGTVGW+LEA+D  E   ++P++ ++PLGTGNDLARCLRWGGGYEGE + K++ K    + VMLDRW I
Sbjct:  138 ESGSAQAEKRADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDR-----------------------------------------------QRSVNQAHKKSQ-MHHHQATHFQITP-PDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKG-GPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIEL-ASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSI 575          

HSP 2 Score: 257.684 bits (657), Expect = 1.301e-70
Identity = 125/220 (56.82%), Postives = 163/220 (74.09%), Query Frame = 1
Query: 2476 MPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            +P NIINNYFS+GVDA+I  KFH+ REK+P KFNSRM+N+ +Y E  TSE   A+CK+LHE I+I+CDGV LDL  G +L+G+A+LNIP  +GG NLWG  +S  +I+K+     K KK  +G  E    S  S   +L+ A+QD GD L+EV+GLEN +H G V  G R+SG+RLAQC EV+I+T++ FPMQIDGEPW+Q  CTI +T+KNQVPM+MAP
Sbjct:  877 VPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSV--DLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAP 1094          
BLAST of Diacylglycerol kinase vs. Ensembl Fly
Match: Dgk (gene:FBgn0085390 transcript:FBtr0112580)

HSP 1 Score: 488.419 bits (1256), Expect = 7.844e-153
Identity = 245/478 (51.26%), Postives = 316/478 (66.11%), Query Frame = 1
Query:  850 NLDIVNRPLTDQD----KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLD-TNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSD--QLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPD--TKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            ++ ++ R L+  D    K+ +KD++CYLSLLE+G+PEDKLEFMFRLYDTD NGVLD +E++ I++QMM VA YL WD  EL PILQ+MM EIDYD DG V+L+EW+RGGMT IPLLVLLG+D T +++DG HVW+LKHFSK  +C+LC  +LV  G++GLCCV C YTVHERCVQ+APASCI TY K+KK      L HHW+E +   +C KC+K  K     +G  CRWC   +H  C  S ++ C LG+  E I+PP  I P+VL+R                                               Q S +Q   + Q ++H+Q + FQI   PD  + PLLVF+NPKSGG+QG RI+ KFQY+LNPRQVYDL K  GP  GL  F+D+  FR++ CGGDGTVGW+LEA+D  E   ++P++ ++PLGTGNDLARCLRWGGGYEGE + K++ K    + VMLDRW I
Sbjct:  252 DIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDR-----------------------------------------------QRSVNQAHKKSQ-MHHHQATHFQITP-PDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKG-GPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIEL-ASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSI 678          

HSP 2 Score: 258.455 bits (659), Expect = 1.533e-70
Identity = 125/220 (56.82%), Postives = 163/220 (74.09%), Query Frame = 1
Query: 2476 MPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            +P NIINNYFS+GVDA+I  KFH+ REK+P KFNSRM+N+ +Y E  TSE   A+CK+LHE I+I+CDGV LDL  G +L+G+A+LNIP  +GG NLWG  +S  +I+K+     K KK  +G  E    S  S   +L+ A+QD GD L+EV+GLEN +H G V  G R+SG+RLAQC EV+I+T++ FPMQIDGEPW+Q  CTI +T+KNQVPM+MAP
Sbjct:  980 VPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSV--DLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAP 1197          
BLAST of Diacylglycerol kinase vs. Ensembl Fly
Match: Dgk (gene:FBgn0085390 transcript:FBtr0346954)

HSP 1 Score: 483.411 bits (1243), Expect = 4.430e-152
Identity = 239/487 (49.08%), Postives = 314/487 (64.48%), Query Frame = 1
Query:  817 EKSALTLSKSNNLDIVNRPLTDQD----KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLD-TNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSD--QLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            E  +    K  ++ ++ R L+  D    K+ +KD++CYLSLLE+G+PEDKLEFMFRLYDTD NGVLD +E++ I++QMM VA YL WD  EL PILQ+MM EIDYD DG V+L+EW+RGGMT IPLLVLLG+D T +++DG HVW+LKHFSK  +C+LC  +LV  G++GLCCV C YTVHERCVQ+APASCI TY K+KK      L HHW+E +   +C KC+K  K     +G  CRWC   +H  C  S ++ C LG+  E I+PP  I P+VL+R+++        + +  ++ +   +  DE+ C                                               PLLVF+NPKSGG+QG RI+ KFQY+LNPRQVYDL K  GP  GL  F+D+  FR++ CGGDGTVGW+LEA+D  E   ++P++ ++PLGTGNDLARCLRWGGGYEGE + K++ K    + VMLDRW I
Sbjct:  138 ESGSAQAEKRADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRS--------VNQAHKATHFQITPPDELSC-----------------------------------------------PLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKG-GPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIEL-ASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSI 567          

HSP 2 Score: 257.684 bits (657), Expect = 1.355e-70
Identity = 125/220 (56.82%), Postives = 163/220 (74.09%), Query Frame = 1
Query: 2476 MPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            +P NIINNYFS+GVDA+I  KFH+ REK+P KFNSRM+N+ +Y E  TSE   A+CK+LHE I+I+CDGV LDL  G +L+G+A+LNIP  +GG NLWG  +S  +I+K+     K KK  +G  E    S  S   +L+ A+QD GD L+EV+GLEN +H G V  G R+SG+RLAQC EV+I+T++ FPMQIDGEPW+Q  CTI +T+KNQVPM+MAP
Sbjct:  869 VPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSV--DLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAP 1086          
BLAST of Diacylglycerol kinase vs. Ensembl Fly
Match: Dgk (gene:FBgn0085390 transcript:FBtr0330238)

HSP 1 Score: 483.797 bits (1244), Expect = 8.455e-152
Identity = 239/487 (49.08%), Postives = 314/487 (64.48%), Query Frame = 1
Query:  817 EKSALTLSKSNNLDIVNRPLTDQD----KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLD-TNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSD--QLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            E  +    K  ++ ++ R L+  D    K+ +KD++CYLSLLE+G+PEDKLEFMFRLYDTD NGVLD +E++ I++QMM VA YL WD  EL PILQ+MM EIDYD DG V+L+EW+RGGMT IPLLVLLG+D T +++DG HVW+LKHFSK  +C+LC  +LV  G++GLCCV C YTVHERCVQ+APASCI TY K+KK      L HHW+E +   +C KC+K  K     +G  CRWC   +H  C  S ++ C LG+  E I+PP  I P+VL+R+++        + +  ++ +   +  DE+ C                                               PLLVF+NPKSGG+QG RI+ KFQY+LNPRQVYDL K  GP  GL  F+D+  FR++ CGGDGTVGW+LEA+D  E   ++P++ ++PLGTGNDLARCLRWGGGYEGE + K++ K    + VMLDRW I
Sbjct:  138 ESGSAQAEKRADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRS--------VNQAHKATHFQITPPDELSC-----------------------------------------------PLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKG-GPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIEL-ASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSI 567          

HSP 2 Score: 257.684 bits (657), Expect = 1.700e-70
Identity = 125/220 (56.82%), Postives = 163/220 (74.09%), Query Frame = 1
Query: 2476 MPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            +P NIINNYFS+GVDA+I  KFH+ REK+P KFNSRM+N+ +Y E  TSE   A+CK+LHE I+I+CDGV LDL  G +L+G+A+LNIP  +GG NLWG  +S  +I+K+     K KK  +G  E    S  S   +L+ A+QD GD L+EV+GLEN +H G V  G R+SG+RLAQC EV+I+T++ FPMQIDGEPW+Q  CTI +T+KNQVPM+MAP
Sbjct:  869 VPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSV--DLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAP 1086          
BLAST of Diacylglycerol kinase vs. Ensembl Fly
Match: Dgk (gene:FBgn0085390 transcript:FBtr0308589)

HSP 1 Score: 484.567 bits (1246), Expect = 2.902e-151
Identity = 239/487 (49.08%), Postives = 314/487 (64.48%), Query Frame = 1
Query:  817 EKSALTLSKSNNLDIVNRPLTDQD----KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLD-TNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSD--QLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            E  +    K  ++ ++ R L+  D    K+ +KD++CYLSLLE+G+PEDKLEFMFRLYDTD NGVLD +E++ I++QMM VA YL WD  EL PILQ+MM EIDYD DG V+L+EW+RGGMT IPLLVLLG+D T +++DG HVW+LKHFSK  +C+LC  +LV  G++GLCCV C YTVHERCVQ+APASCI TY K+KK      L HHW+E +   +C KC+K  K     +G  CRWC   +H  C  S ++ C LG+  E I+PP  I P+VL+R+++        + +  ++ +   +  DE+ C                                               PLLVF+NPKSGG+QG RI+ KFQY+LNPRQVYDL K  GP  GL  F+D+  FR++ CGGDGTVGW+LEA+D  E   ++P++ ++PLGTGNDLARCLRWGGGYEGE + K++ K    + VMLDRW I
Sbjct:  268 ESGSAQAEKRADIKLLARKLSHFDPLTLKVPLKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQEMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCITTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGEYSELIVPPTAICPAVLDRQRS--------VNQAHKATHFQITPPDELSC-----------------------------------------------PLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKG-GPKEGLTLFKDLPRFRVICCGGDGTVGWVLEAMDSIEL-ASQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKFRRASTVMLDRWSI 697          

HSP 2 Score: 257.684 bits (657), Expect = 2.361e-70
Identity = 125/220 (56.82%), Postives = 163/220 (74.09%), Query Frame = 1
Query: 2476 MPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            +P NIINNYFS+GVDA+I  KFH+ REK+P KFNSRM+N+ +Y E  TSE   A+CK+LHE I+I+CDGV LDL  G +L+G+A+LNIP  +GG NLWG  +S  +I+K+     K KK  +G  E    S  S   +L+ A+QD GD L+EV+GLEN +H G V  G R+SG+RLAQC EV+I+T++ FPMQIDGEPW+Q  CTI +T+KNQVPM+MAP
Sbjct:  999 VPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRIRKSAGPFGKSKKLRAGDKEFSATSFNSV--DLSVAIQDFGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAP 1216          
BLAST of Diacylglycerol kinase vs. Ensembl Zebrafish
Match: dgkaa (diacylglycerol kinase, alpha a [Source:ZFIN;Acc:ZDB-GENE-060616-305])

HSP 1 Score: 599.742 bits (1545), Expect = 0.000e+0
Identity = 336/754 (44.56%), Postives = 418/754 (55.44%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIE-NHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            ++ +KD+ CY SLLE G+P DKL+F F+LYD DGNG LD SEV+ II+QMM+ A YL WD  EL P+L+ MM  ID D+ G V+ EEW  GG+ N+PLLVLLGL    + DG+H+W++KHF+K  +C++C  +L+   +QGLCC  C YTVH RC    PA C  TY  +KK   +  H W+  N    KC  C+K  K L   +G  C WC    H  C      TCD G  K HILPP  IYP V ER    KN      +  SE     D                                              +I  +PDT+PLLVF+NPKSGGKQG R++ KFQY+LNPRQVY+L  + GP  GL FFRDV ++RIL+CGGDGTVGW+L+AIDK      RP VA+LPLGTGNDLARCLRWGGGY+G  L +IL  I +     +DRW I  +                                            EDSQ    E GD               +P  IINNYFSIGVDASIAH+FH +REKHP+KFNSRM+N+ +Y E  TSE ++A+CK L E + I C G  LDL     LEGIAILNIPS++GG NLWG                  +   S R + +        E L  + QDM D  LEVVGLE AM  G +  G +S+  RLA+  ++ IRT++  PMQIDGEPW+Q  CTI IT+KNQ  M+M P  K
Sbjct:  135 EVFLKDVSCYFSLLEEGQPRDKLDFAFKLYDRDGNGFLDSSEVDRIIAQMMHAADYLGWDVSELRPVLKDMMTAIDADSSGTVSSEEWVDGGLKNVPLLVLLGLKMTQK-DGQHLWRMKHFNKPVYCNVCQNMLLGLRKQGLCCTCCRYTVHGRCANKNPAPCTRTYVMSKKETGIAVHDWVSGNCDSGKCDGCQKKIKSLHGLTGKRCVWCHTMRHDACATQEPFTCDCGPFKVHILPPWAIYPVVKERPNCIKNGGSFP-DDDSELNTTPDGQV------------------------------------------LRISPIPDTRPLLVFVNPKSGGKQGERVLRKFQYLLNPRQVYNL-SNGGPGPGLSFFRDVPNYRILVCGGDGTVGWILDAIDKANL-PVRPPVAVLPLGTGNDLARCLRWGGGYDGMDLGRILKDIEVSEEGPMDRWSIQVT-------------------------------------------LEDSQ----ERGD--------------PVPYEIINNYFSIGVDASIAHRFHTMREKHPQKFNSRMKNKLWYFEFATSETISASCKKLKECLTIECCGTQLDL-SSLSLEGIAILNIPSMHGGSNLWG------------------EAKKSDRMDQKLPEVIVDPEILKVSPQDMSDKRLEVVGLEGAMEMGQIYTGLKSA-VRLAKTSQITIRTKKPLPMQIDGEPWMQPPCTIHITHKNQASMLMGPQAK 761          
BLAST of Diacylglycerol kinase vs. Ensembl Zebrafish
Match: dgkaa (diacylglycerol kinase, alpha a [Source:ZFIN;Acc:ZDB-GENE-060616-305])

HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0
Identity = 336/754 (44.56%), Postives = 418/754 (55.44%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIE-NHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            ++ +KD+ CY SLLE G+P DKL+F F+LYD DGNG LD SEV+ II+QMM+ A YL WD  EL P+L+ MM  ID D+ G V+ EEW  GG+ N+PLLVLLGL    + DG+H+W++KHF+K  +C++C  +L+   +QGLCC  C YTVH RC    PA C  TY  +KK   +  H W+  N    KC  C+K  K L   +G  C WC    H  C      TCD G  K HILPP  IYP V ER    KN      +  SE     D                                              +I  +PDT+PLLVF+NPKSGGKQG R++ KFQY+LNPRQVY+L  + GP  GL FFRDV ++RIL+CGGDGTVGW+L+AIDK      RP VA+LPLGTGNDLARCLRWGGGY+G  L +IL  I +     +DRW I  +                                            EDSQ    E GD               +P  IINNYFSIGVDASIAH+FH +REKHP+KFNSRM+N+ +Y E  TSE ++A+CK L E + I C G  LDL     LEGIAILNIPS++GG NLWG                  +   S R + +        E L  + QDM D  LEVVGLE AM  G +  G +S+  RLA+  ++ IRT++  PMQIDGEPW+Q  CTI IT+KNQ  M+M P  K
Sbjct:   96 EVFLKDVSCYFSLLEEGQPRDKLDFAFKLYDRDGNGFLDSSEVDRIIAQMMHAADYLGWDVSELRPVLKDMMTAIDADSSGTVSSEEWVDGGLKNVPLLVLLGLKMTQK-DGQHLWRMKHFNKPVYCNVCQNMLLGLRKQGLCCTCCRYTVHGRCANKNPAPCTRTYVMSKKETGIAVHDWVSGNCDSGKCDGCQKKIKSLHGLTGKRCVWCHTMRHDACATQEPFTCDCGPFKVHILPPWAIYPVVKERPNCIKNGGSFP-DDDSELNTTPDGQV------------------------------------------LRISPIPDTRPLLVFVNPKSGGKQGERVLRKFQYLLNPRQVYNL-SNGGPGPGLSFFRDVPNYRILVCGGDGTVGWILDAIDKANL-PVRPPVAVLPLGTGNDLARCLRWGGGYDGMDLGRILKDIEVSEEGPMDRWSIQVT-------------------------------------------LEDSQ----ERGD--------------PVPYEIINNYFSIGVDASIAHRFHTMREKHPQKFNSRMKNKLWYFEFATSETISASCKKLKECLTIECCGTQLDL-SSLSLEGIAILNIPSMHGGSNLWG------------------EAKKSDRMDQKLPEVIVDPEILKVSPQDMSDKRLEVVGLEGAMEMGQIYTGLKSA-VRLAKTSQITIRTKKPLPMQIDGEPWMQPPCTIHITHKNQASMLMGPQAK 722          
BLAST of Diacylglycerol kinase vs. Ensembl Zebrafish
Match: dgkab (diacylglycerol kinase, alpha b [Source:ZFIN;Acc:ZDB-GENE-121105-5])

HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0
Identity = 316/774 (40.83%), Postives = 416/774 (53.75%), Query Frame = 1
Query:  877 TDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPA-KCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISS-------FQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF-------ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFG 3153
            T    + +K + CY S+LE G P DKLE+ F+LYD D NG+LD  EV+ I++QMM  A YL WD  EL P+L+ M+  +D D+ G VTLEEW  GG+ NIPLLVLLGL    + DG+HVW+LK+F++ T+C +C  +++   +QGL C FC YTVH  C       C+ T+ ++K       H WI     + KC  C K  K+   +G  C WC    H  C+      CD G  + HILPP  I                               A D+ DC                        I   GL    + +        QI  + DT PLLVF+NPKSGGKQG R++ KFQY+LNPRQVY+L  + GPN GLQFF++++ FR+L+CGGDGTVGW+L+AIDK      RP VAILPLGTGNDLARCL WGGGYEG  L +IL +I     + +DRW +     AP                                            +D  +EGD               +P +IINNYFSIGVDASIAH+FHV+REKHP+KFNSR RN+ +Y ++ TSE ++A+C++L + + I C GV +DL + + LEG+A+LNIPS++GG NLWG                          E++   +  T+       E L T  QDM D LLEVVGLE+ +  G +  G +S   RLAQ   + +RT +  PMQ+DGEPW+Q  CTI IT+KNQ  M+ AP  K  G
Sbjct:   97 TKDGGVSLKAVSCYFSILEDGNPRDKLEYTFKLYDKDDNGLLDSKEVDKIVAQMMKAAEYLGWDVTELKPVLEDMLKAMDVDSSGAVTLEEWINGGLNNIPLLVLLGLKVADK-DGQHVWRLKNFNRPTYCFVCQNMMLGLRKQGLSCNFCKYTVHSSCANKNHTPCVRTFVRSKAEIGCSAHDWISADCESNKCQICHKKIKI--LTGQRCAWCHSMRHTDCVPLGSSHCDCGSLQHHILPPWAI------------------------------CAVDQADC------------------------ISVSGLGETHLGNITSEGHFLQIFPVMDTNPLLVFVNPKSGGKQGKRVLRKFQYLLNPRQVYNL-DNGGPNPGLQFFQNLQAFRVLVCGGDGTVGWVLDAIDKANM-AIRPPVAILPLGTGNDLARCLNWGGGYEGTDLTEILKQIEESRSIQMDRWSLRV---AP--------------------------------------------VDDADEGD--------------PVPNDIINNYFSIGVDASIAHQFHVMREKHPQKFNSRARNKLWYFQLATSETISASCRNLKDCLSIECCGVPVDLSRLS-LEGVAVLNIPSMHGGSNLWG--------------------------ETKSAEKPKTWQEVRVDPEALQTCSQDMSDELLEVVGLESVLEMGQIYTGLKSKAHRLAQAAHITVRTCKTLPMQVDGEPWMQPPCTIHITHKNQANMLTAPAAKSVG 723          
BLAST of Diacylglycerol kinase vs. Ensembl Zebrafish
Match: dgkaa (diacylglycerol kinase, alpha a [Source:ZFIN;Acc:ZDB-GENE-060616-305])

HSP 1 Score: 551.206 bits (1419), Expect = 0.000e+0
Identity = 323/755 (42.78%), Postives = 403/755 (53.38%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIE-NHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLT-ATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            ++ +KD+ CY SLLE G+P DKL+F F+LYD DGNG LD SEV+ II+QMM+ A YL WD  EL P+L+ MM  ID D+ G V+ EEW  GG+ N+PL     L +  + DG+H+W++KHF+K  +C++C  +L+   +QGLCC  C YTVH RC    PA C  TY  +KK   +  H W+  N    KC  C+K  K L   +G  C WC    H  C      TCD G  K HILPP  IYP V ER    KN      +  SE     D                                              +I  +PDT+PLLVF+NPKSGGKQG R++ KFQY+LNPRQVY+L  + GP  GL FFRDV ++RIL+CGGDGTVGW+L+AIDK      RP VA+LPLGTGNDLARCLRWGGGY+G  L +IL  I +     +DRW I  +                                            EDSQ    E GD               +P  IINNYFSIGVDASIAH+FH +REKHP+KFN        Y+++G    LT AT   L +     C G  LDL     LEGIAILNIPS++GG NLWG                  +   S R + +        E L  + QDM D  LEVVGLE AM  G +  G +S+  RLA+  ++ IRT++  PMQIDGEPW+Q  CTI IT+KNQ  M+M P  K
Sbjct:   96 EVFLKDVSCYFSLLEEGQPRDKLDFAFKLYDRDGNGFLDSSEVDRIIAQMMHAADYLGWDVSELRPVLKDMMTAIDADSSGTVSSEEWVDGGLKNVPL-----LSSMTQKDGQHLWRMKHFNKPVYCNVCQNMLLGLRKQGLCCTCCRYTVHGRCANKNPAPCTRTYVMSKKETGIAVHDWVSGNCDSGKCDGCQKKIKSLHGLTGKRCVWCHTMRHDACATQEPFTCDCGPFKVHILPPWAIYPVVKERPNCIKNGGSFP-DDDSELNTTPDGQV------------------------------------------LRISPIPDTRPLLVFVNPKSGGKQGERVLRKFQYLLNPRQVYNL-SNGGPGPGLSFFRDVPNYRILVCGGDGTVGWILDAIDKANL-PVRPPVAVLPLGTGNDLARCLRWGGGYDGMDLGRILKDIEVSEEGPMDRWSIQVT-------------------------------------------LEDSQ----ERGD--------------PVPYEIINNYFSIGVDASIAHRFHTMREKHPQKFN------IHYLKMGALFRLTSATLSGLMQ-----CCGTQLDL-SSLSLEGIAILNIPSMHGGSNLWG------------------EAKKSDRMDQKLPEVIVDPEILKVSPQDMSDKRLEVVGLEGAMEMGQIYTGLKSA-VRLAKTSQITIRTKKPLPMQIDGEPWMQPPCTIHITHKNQASMLMGPQAK 708          
BLAST of Diacylglycerol kinase vs. Ensembl Zebrafish
Match: dgkg (diacylglycerol kinase, gamma [Source:ZFIN;Acc:ZDB-GENE-081105-39])

HSP 1 Score: 474.167 bits (1219), Expect = 2.196e-153
Identity = 240/455 (52.75%), Postives = 301/455 (66.15%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            + +KD++CYLSLLE G PEDKLEFMFRLYDTDGNG+LD SE++ II+QM++VA YL WD+ EL PIL++MM EIDYD DG VTLEEW RGGMT IPLLVLLG++TNV++DG+H+WKLKHF+K  +C+ CH +L+   +QGLCC  C YTVHERCV    A CI TY K+++    ++H W+E +  AKC KC +S K     +G HC WC+ T+H  C  + +  CD G  ++H L P  I P VL+R    K  +     ++S S +  D+                            QCF   +     Q  + QI  LP T PLLV +NPKSGG+QG R++ KFQY+LNPRQVY L +  GP  GL FFRDV DFR+L CGGDG+VGW+L+ IDK  +  + P VAILPLGTGNDLARCLRWGGGYEG  L K L  I   T V+LDRW I
Sbjct:   27 VFLKDIVCYLSLLERGTPEDKLEFMFRLYDTDGNGLLDSSELDRIINQMVHVAEYLEWDSTELRPILKEMMEEIDYDRDGTVTLEEWIRGGMTTIPLLVLLGMETNVQEDGQHIWKLKHFNKPAYCNYCHTMLLGVRKQGLCCALCKYTVHERCVSKDIAPCISTYAKSRRHTNAMQHVWMEGNSSAKCDKCHRSIKCYQGLTGLHCVWCQITLHNKCASNMKPECDGGPLRDHTLLPSYICPVVLDRHSAVKRGE----GESSPSTSPEDTG---------------------------QCF---KFTGDGQ--ALQITPLPGTHPLLVLVNPKSGGRQGERVLRKFQYLLNPRQVYSLERG-GPMAGLNFFRDVPDFRVLACGGDGSVGWILDCIDKASFARH-PPVAILPLGTGNDLARCLRWGGGYEGGSLVKFLRDIEHSTEVLLDRWNI 443          

HSP 2 Score: 245.743 bits (626), Expect = 7.578e-69
Identity = 121/225 (53.78%), Postives = 155/225 (68.89%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            D +P +I+NNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+ +Y E GT+E ++ATCK L+E I++ CDG++LDL   + LEGIA+LNIPS++GG NLWG         +     P          E    S  +  + L   VQD+ D LLEVVGLE A+  G +  G +S+G+RLAQC  V IRT R  PMQIDGEPW+Q  CTI IT+KNQVPM++ PP K
Sbjct:  455 DPVPYSIVNNYFSIGVDASIAHRFHLMREKHPEKFNSRMKNKLWYFEFGTTETISATCKKLNETIEVECDGIILDLSSTS-LEGIAVLNIPSMHGGSNLWGETKKRRNYNRMSKKVP----------ERMTGSTVTDAKELKFCVQDLSDQLLEVVGLEGAIEMGQIYTGLKSAGRRLAQCSNVTIRTSRLLPMQIDGEPWMQPPCTIRITHKNQVPMLLGPPQK 668          
BLAST of Diacylglycerol kinase vs. Ensembl Xenopus
Match: DGKA (diacylglycerol kinase alpha [Source:NCBI gene;Acc:100124931])

HSP 1 Score: 430.639 bits (1106), Expect = 1.499e-135
Identity = 227/457 (49.67%), Postives = 291/457 (63.68%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPA-KCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            +I + D+ CY SLLESG  EDKL F F+LYD D NG+LD SEV+ II+QMM VA YL+WD  EL P+L++MM ++DYD +G V+ EEW +GG+T +PLLVLLG++ N++DDG+H+W+LKHF++  +C++C  +L    +QGLCC FC +TVHERC + AP SCI TY K++K      H W+     A KC KC+K  K   + +G  C WC   +H  C  S    CD G  K+HILPP CIYP +LER+ + KN              LA S                           SQ F   +G         +I  LP T PLLVF+NPKSGGKQG R++ KFQY+LNPRQV++L+K  GP+ GL FFRDV ++RIL+CGGDGTVGW+L+AIDK      RP VA+LPLGTGNDLARCL WGGGY+GE L K L  I     + +DRW I
Sbjct:  132 RISLNDVSCYFSLLESGTAEDKLAFTFKLYDKDSNGLLDSSEVDRIITQMMRVAEYLDWDITELKPMLEEMMRQVDYDGNGIVSYEEWVKGGLTTVPLLVLLGMEVNIKDDGQHLWRLKHFNRPVYCNVCEALLFGVRKQGLCCTFCKFTVHERCARKAPNSCISTYAKSRKDFSEQAHVWMRGGCEASKCDKCQKKIKSFQSLTGQRCVWCHTKVHDECASS--PLCDGGILKDHILPPACIYPVILERQNSQKNL-------------LASS------------------------EEASQTFSTPEG------QVLRIAPLPGTHPLLVFVNPKSGGKQGERVLRKFQYLLNPRQVHNLIKG-GPSPGLNFFRDVPEYRILVCGGDGTVGWILDAIDKANL-PYRPPVAVLPLGTGNDLARCLSWGGGYDGENLMKFLKDIEKSVPIKMDRWNI 541          

HSP 2 Score: 210.305 bits (534), Expect = 5.651e-56
Identity = 115/228 (50.44%), Postives = 142/228 (62.28%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFE------NLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            D +P  IINNYFSIGVDASIAH+FH +REK+PEKFNSRM+N+ +Y E  TSE + +TCK L E I I C G  L L     LEGIA++NIPS++GG NLWG                   ++     E R  S  +  E       L + VQD+ D  LEVVGLE A+  G +  G +S+GKRLA C E+ IRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+ AP
Sbjct:  553 DPVPYEIINNYFSIGVDASIAHRFHNMREKYPEKFNSRMKNKLWYFEFATSETVFSTCKKLKEVITIECCGSPLKLSS-LSLEGIAVINIPSMHGGSNLWG-------------------ETKKHWGEPRNASCHNAPEAITDPDALKSCVQDLSDKRLEVVGLEGAIEMGQIYTGLKSAGKRLATCSELTIRTTKPLPMQIDGEPWMQPPCTIKITHKNQAPMLRAP 760          
BLAST of Diacylglycerol kinase vs. Ensembl Xenopus
Match: DGKA (diacylglycerol kinase alpha [Source:NCBI gene;Acc:100124931])

HSP 1 Score: 429.869 bits (1104), Expect = 1.770e-135
Identity = 227/457 (49.67%), Postives = 291/457 (63.68%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPA-KCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            +I + D+ CY SLLESG  EDKL F F+LYD D NG+LD SEV+ II+QMM VA YL+WD  EL P+L++MM ++DYD +G V+ EEW +GG+T +PLLVLLG++ N++DDG+H+W+LKHF++  +C++C  +L    +QGLCC FC +TVHERC + AP SCI TY K++K      H W+     A KC KC+K  K   + +G  C WC   +H  C  S    CD G  K+HILPP CIYP +LER+ + KN              LA S                           SQ F   +G         +I  LP T PLLVF+NPKSGGKQG R++ KFQY+LNPRQV++L+K  GP+ GL FFRDV ++RIL+CGGDGTVGW+L+AIDK      RP VA+LPLGTGNDLARCL WGGGY+GE L K L  I     + +DRW I
Sbjct:  109 RISLNDVSCYFSLLESGTAEDKLAFTFKLYDKDSNGLLDSSEVDRIITQMMRVAEYLDWDITELKPMLEEMMRQVDYDGNGIVSYEEWVKGGLTTVPLLVLLGMEVNIKDDGQHLWRLKHFNRPVYCNVCEALLFGVRKQGLCCTFCKFTVHERCARKAPNSCISTYAKSRKDFSEQAHVWMRGGCEASKCDKCQKKIKSFQSLTGQRCVWCHTKVHDECASS--PLCDGGILKDHILPPACIYPVILERQNSQKNL-------------LASS------------------------EEASQTFSTPEG------QVLRIAPLPGTHPLLVFVNPKSGGKQGERVLRKFQYLLNPRQVHNLIKG-GPSPGLNFFRDVPEYRILVCGGDGTVGWILDAIDKANL-PYRPPVAVLPLGTGNDLARCLSWGGGYDGENLMKFLKDIEKSVPIKMDRWNI 518          

HSP 2 Score: 209.92 bits (533), Expect = 5.403e-56
Identity = 115/228 (50.44%), Postives = 142/228 (62.28%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFE------NLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            D +P  IINNYFSIGVDASIAH+FH +REK+PEKFNSRM+N+ +Y E  TSE + +TCK L E I I C G  L L     LEGIA++NIPS++GG NLWG                   ++     E R  S  +  E       L + VQD+ D  LEVVGLE A+  G +  G +S+GKRLA C E+ IRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+ AP
Sbjct:  530 DPVPYEIINNYFSIGVDASIAHRFHNMREKYPEKFNSRMKNKLWYFEFATSETVFSTCKKLKEVITIECCGSPLKLSS-LSLEGIAVINIPSMHGGSNLWG-------------------ETKKHWGEPRNASCHNAPEAITDPDALKSCVQDLSDKRLEVVGLEGAIEMGQIYTGLKSAGKRLATCSELTIRTTKPLPMQIDGEPWMQPPCTIKITHKNQAPMLRAP 737          
BLAST of Diacylglycerol kinase vs. Ensembl Xenopus
Match: DGKA (diacylglycerol kinase alpha [Source:NCBI gene;Acc:100124931])

HSP 1 Score: 380.948 bits (977), Expect = 1.295e-117
Identity = 212/468 (45.30%), Postives = 277/468 (59.19%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLE-----------FMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPA-KCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            +I + D+ CY SLLESG  EDKL             +F       +  +   EV+ II+QMM VA YL+WD  EL P+L++MM ++DYD +G V+ EEW +GG+T +PLLVLLG++ N++DDG+H+W+LKHF++  +C++C  +L    +QGLCC FC +TVHERC + AP SCI TY K++K      H W+     A KC KC+K  K   + +G  C WC   +H  C  S    CD G  K+HILPP CIYP +L    + KN                                                     G +H +IS+  I  LP T PLLVF+NPKSGGKQG R++ KFQY+LNPRQV++L+K  GP+ GL FFRDV ++RIL+CGGDGTVGW+L+AIDK      RP VA+LPLGTGNDLARCL WGGGY+GE L K L  I     + +DRW I
Sbjct:   67 RISLNDVSCYFSLLESGTAEDKLNCLPNYNTCLYTHVFYTKHLSHHLWVFPQEVDRIITQMMRVAEYLDWDITELKPMLEEMMRQVDYDGNGIVSYEEWVKGGLTTVPLLVLLGMEVNIKDDGQHLWRLKHFNRPVYCNVCEALLFGVRKQGLCCTFCKFTVHERCARKAPNSCISTYAKSRKDFSEQAHVWMRGGCEASKCDKCQKKIKSFQSLTGQRCVWCHTKVHDECASS--PLCDGGILKDHILPPACIYPVILVSPLSHKN-----------------------------------------------------GSDHMKISAL-IAPLPGTHPLLVFVNPKSGGKQGERVLRKFQYLLNPRQVHNLIKG-GPSPGLNFFRDVPEYRILVCGGDGTVGWILDAIDKANL-PYRPPVAVLPLGTGNDLARCLSWGGGYDGENLMKFLKDIEKSVPIKMDRWNI 476          

HSP 2 Score: 188.348 bits (477), Expect = 4.687e-49
Identity = 105/215 (48.84%), Postives = 131/215 (60.93%), Query Frame = 1
Query: 2467 SDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFE------NLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTI 3093
             D +P  IINNYFSIGVDASIAH+FH +REK+PEKFNSRM+N+ +Y E  TSE + +TCK L E I I C G  L L     LEGIA++NIPS++GG NLWG                   ++     E R  S  +  E       L + VQD+ D  LEVVGLE A+  G +  G +S+GKRLA C E+ IRT +  PMQIDGEPW+Q  CT+
Sbjct:  487 GDPVPYEIINNYFSIGVDASIAHRFHNMREKYPEKFNSRMKNKLWYFEFATSETVFSTCKKLKEVITIECCGSPLKLSS-LSLEGIAVINIPSMHGGSNLWG-------------------ETKKHWGEPRNASCHNAPEAITDPDALKSCVQDLSDKRLEVVGLEGAIEMGQIYTGLKSAGKRLATCSELTIRTTKPLPMQIDGEPWMQPPCTV 681          
BLAST of Diacylglycerol kinase vs. Ensembl Xenopus
Match: DGKZ (diacylglycerol kinase, zeta [Source:NCBI gene;Acc:100496839])

HSP 1 Score: 270.781 bits (691), Expect = 2.014e-75
Identity = 195/617 (31.60%), Postives = 266/617 (43.11%), Query Frame = 1
Query: 1318 CVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPA-KCIKCKKSTKLGAKSGFH--------CRWCKRTIHK---CCM-ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            C  C   VH  C++      I            + HHW+     A KC +C K  +   K  FH        C WCK+  H    C M     + C LG +   I+PP  I   +  R+  +  K     ++ S    L+  A++E                        + FI             +    P  KP+LVF+NPKSGG QG +I+  F + LNPRQV+DL +  GP   L+ +R V   RIL CGGDGTVGW+L A+D+       P VAILPLGTGNDLAR L WGGGY  E L KIL+ +  G VV LDRW +                             RN    +D                  ++GD  +D          ++P ++ NNYFS+G DA +  +FH  RE +PEKFNSR RN+ FY     S+  T + + L ++I ++CDGV L  + +   L+ +  LNIP    G   WG                     + G     E  R               D  +EV+G   A  A L  GG    G+RL QCREV++ T +  PMQ+DGEP       I I+ +NQ  ++
Sbjct:  117 CAACKIVVHTPCIEQLEKPVI------------VRHHWVHRRRQAGKCRQCGKGFQ--QKFAFHSKEIVAISCSWCKQAYHSKVSCFMFHHIEEPCSLGVHAAVIVPPTWI---IRVRRPQSSLKSSKKKKRPSFKRKLSKKASEE---------------------PRQRPFI------------IRPAPCPLMKPVLVFVNPKSGGNQGVKILQSFMWYLNPRQVFDLSQG-GPKEALEMYRKVPSLRILACGGDGTVGWILSALDQLRLFPP-PPVAILPLGTGNDLARTLNWGGGYTDEPLSKILSHVEEGIVVQLDRWNLV--------------------------VERNPEAWED------------------DKGDGATD----------KLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAVSDFFTGSSRDLAKHIRVVCDGVDLTAKIQDLKLQCLVFLNIPRYCAGTMPWG---------------------NPGEHHDFEPQRHD-------------DGCIEVIGFTMASLAALQVGG---HGERLHQCREVLLTTSKSIPMQVDGEPCKLGPSVIKISLRNQANLV 590          
BLAST of Diacylglycerol kinase vs. Ensembl Xenopus
Match: DGKZ (diacylglycerol kinase, zeta [Source:NCBI gene;Acc:100496839])

HSP 1 Score: 270.396 bits (690), Expect = 2.221e-75
Identity = 195/624 (31.25%), Postives = 270/624 (43.27%), Query Frame = 1
Query: 1318 CVFCLYTVHERCVQNA-------PASCIHTYTKTKKSDQLLEHHWIENHYPA-KCIKCKKSTKLGAKSGFH--------CRWCKRTIHK---CCM-ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            C  C   VH  C++           S   + ++  +   ++ HHW+     A KC +C K      K  FH        C WCK+  H    C M     + C LG +   I+PP  I   +  R+  +  K     ++ S    L+  A++E                        + FI             +    P  KP+LVF+NPKSGG QG +I+  F + LNPRQV+DL +  GP   L+ +R V   RIL CGGDGTVGW+L A+D+       P VAILPLGTGNDLAR L WGGGY  E L KIL+ +  G VV LDRW +                             RN    +D                  ++GD  +D          ++P ++ NNYFS+G DA +  +FH  RE +PEKFNSR RN+ FY     S+  T + + L ++I ++CDGV L  + +   L+ +  LNIP    G   WG                     + G     E  R               D  +EV+G   A  A L  GG    G+RL QCREV++ T +  PMQ+DGEP       I I+ +NQ  ++
Sbjct:   91 CAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNIREPVIVRHHWVHRRRQAGKCRQCGKG--FQQKFAFHSKEIVAISCSWCKQAYHSKVSCFMFHHIEEPCSLGVHAAVIVPPTWI---IRVRRPQSSLKSSKKKKRPSFKRKLSKKASEE---------------------PRQRPFI------------IRPAPCPLMKPVLVFVNPKSGGNQGVKILQSFMWYLNPRQVFDLSQG-GPKEALEMYRKVPSLRILACGGDGTVGWILSALDQLRLFPP-PPVAILPLGTGNDLARTLNWGGGYTDEPLSKILSHVEEGIVVQLDRWNLV--------------------------VERNPEAWED------------------DKGDGATD----------KLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAVSDFFTGSSRDLAKHIRVVCDGVDLTAKIQDLKLQCLVFLNIPRYCAGTMPWG---------------------NPGEHHDFEPQRHD-------------DGCIEVIGFTMASLAALQVGG---HGERLHQCREVLLTTSKSIPMQVDGEPCKLGPSVIKISLRNQANLV 583          
BLAST of Diacylglycerol kinase vs. Ensembl Mouse
Match: Dgkg (diacylglycerol kinase, gamma [Source:MGI Symbol;Acc:MGI:105060])

HSP 1 Score: 658.292 bits (1697), Expect = 0.000e+0
Identity = 349/767 (45.50%), Postives = 454/767 (59.19%), Query Frame = 1
Query:  877 TDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGL-DTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFE--NLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKK--FGLR 3159
            +D   + +KD++CYLSL+E+G+P+DKLEFMFRLYD+D NG+LD++E++ I+SQM++VA YL WD  EL PIL++M+  +DYD DG V+L+EW  GGMT IPLLVLLG+ D+  + DG+H W LKHF K T+C+ C  +L+  G+QGLCC++C YTVH+RCV      C+ T +K K+S ++++H W+E +   KC +C KS K   + +  HC WC+ T H+ C  S    CD G+ K+HIL P  I P   +R                                               Q   S   +  +G     ++ ++I   P T PLLV +NPKSGG+QG RI+ KF Y+LNP QV++L  + GP  GL FF D  DFR+L CGGDGTVGW+L+ IDK  +  + P VA+LPLGTGNDLARCLRWGGGYEG  L KIL +I    +VMLDRW +        PR E                                           E GD              ++P NI+NNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+ +Y E GTSE   ATCK LH++I++ CDGV +DL    +LEGIAILNIPS+YGG NLWG                   ++   R   RE SR+S  +   L   VQD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  V IRT +  PMQ+DGEPW+Q  CTI IT+KNQ PM+M PP K   F LR
Sbjct:  151 SDAPVVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENGLLDQAEMDQIVSQMLHVAQYLEWDPTELRPILKEMLQGMDYDKDGFVSLQEWINGGMTTIPLLVLLGMDDSGSKGDGRHAWTLKHFKKPTYCNFCRAMLMGVGKQGLCCIYCKYTVHQRCVSKTIHGCVKTNSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELS--TVCDGGELKDHILLPTSICPVSGDR-----------------------------------------------QGGKSDGSVAAKG---ELVTQYKIIPSPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPEQVFNL-DNGGPTPGLNFFHDTPDFRVLACGGDGTVGWILDCIDKANFTKH-PPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEV-----MPREE------------------------------------------VENGD--------------QVPYNIMNNYFSIGVDASIAHRFHMMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDL-SNIFLEGIAILNIPSMYGGTNLWG-------------------ETKKNRAVIRE-SRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTNKLLPMQVDGEPWMQPQCTIKITHKNQAPMMMGPPQKSSFFSLR 781          

HSP 2 Score: 58.9214 bits (141), Expect = 3.992e-8
Identity = 36/107 (33.64%), Postives = 54/107 (50.47%), Query Frame = 1
Query:  118 EDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRR-------HSNLEIYNCEPNKAPTLKSKINEDNGKR 417
            E+F+QLQ +   +   ++DV+  EF  G  L QYDP KPI ++ +  F+ AYL  + PQ +   LFL+F ++       H      + EPN      S  N DN  +
Sbjct:   12 EEFDQLQKYSEYSSKKIKDVL-AEFNEGGSLRQYDPHKPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRQETPDHPKEGASSSEPN-----VSDYNSDNAAK 112          
BLAST of Diacylglycerol kinase vs. Ensembl Mouse
Match: Dgkg (diacylglycerol kinase, gamma [Source:MGI Symbol;Acc:MGI:105060])

HSP 1 Score: 610.912 bits (1574), Expect = 0.000e+0
Identity = 336/766 (43.86%), Postives = 432/766 (56.40%), Query Frame = 1
Query:  877 TDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGL-DTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFE--NLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKK--FGLR 3159
            +D   + +KD++CYLSL+E+G+P+DKLEFMFRLYD+D NG+LD++E++ I+SQM++VA YL WD  EL PIL++M+  +DYD DG V+L+EW  GGMT IPLLVLLG+ D+  + DG+H W LKHF K T+C+ C  +L+  G+QGLCC++C YTVH+RCV      C+ T +K K+S +                KC+ ST                            CD G+ K+HIL P  I P   +R                                               Q   S   +  +G     ++ ++I   P T PLLV +NPKSGG+QG RI+ KF Y+LNP QV++L  + GP  GL FF D  DFR+L CGGDGTVGW+L+ IDK  +  + P VA+LPLGTGNDLARCLRWGGGYEG  L KIL +I    +VMLDRW +        PR E                                           E GD              ++P NI+NNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+ +Y E GTSE   ATCK LH++I++ CDGV +DL    +LEGIAILNIPS+YGG NLWG                   ++   R   RE SR+S  +   L   VQD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  V IRT +  PMQ+DGEPW+Q  CTI IT+KNQ PM+M PP K   F LR
Sbjct:  151 SDAPVVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENGLLDQAEMDQIVSQMLHVAQYLEWDPTELRPILKEMLQGMDYDKDGFVSLQEWINGGMTTIPLLVLLGMDDSGSKGDGRHAWTLKHFKKPTYCNFCRAMLMGVGKQGLCCIYCKYTVHQRCVSKTIHGCVKTNSKAKRSGEFHR-------------KCELST---------------------------VCDGGELKDHILLPTSICPVSGDR-----------------------------------------------QGGKSDGSVAAKG---ELVTQYKIIPSPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPEQVFNL-DNGGPTPGLNFFHDTPDFRVLACGGDGTVGWILDCIDKANFTKH-PPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEV-----MPREE------------------------------------------VENGD--------------QVPYNIMNNYFSIGVDASIAHRFHMMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDL-SNIFLEGIAILNIPSMYGGTNLWG-------------------ETKKNRAVIRE-SRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTNKLLPMQVDGEPWMQPQCTIKITHKNQAPMMMGPPQKSSFFSLR 742          

HSP 2 Score: 58.5362 bits (140), Expect = 4.251e-8
Identity = 36/107 (33.64%), Postives = 54/107 (50.47%), Query Frame = 1
Query:  118 EDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRR-------HSNLEIYNCEPNKAPTLKSKINEDNGKR 417
            E+F+QLQ +   +   ++DV+  EF  G  L QYDP KPI ++ +  F+ AYL  + PQ +   LFL+F ++       H      + EPN      S  N DN  +
Sbjct:   12 EEFDQLQKYSEYSSKKIKDVL-AEFNEGGSLRQYDPHKPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRQETPDHPKEGASSSEPN-----VSDYNSDNAAK 112          
BLAST of Diacylglycerol kinase vs. Ensembl Mouse
Match: Dgkb (diacylglycerol kinase, beta [Source:MGI Symbol;Acc:MGI:2442474])

HSP 1 Score: 504.212 bits (1297), Expect = 2.791e-163
Identity = 260/466 (55.79%), Postives = 308/466 (66.09%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVL-----------ERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E II QMM+VA YL WD  EL PIL +MM EIDYD DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+KK+  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL           ER+ TAK        K   S    + A D+                   Q +NS   +  QGL        QI  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  125 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTMPSAGASVPEERQSTAK--------KEKSSSQQPNKATDKNKM----------------QRANS-VTMDGQGL--------QITPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKF 555          

HSP 2 Score: 254.988 bits (650), Expect = 3.546e-71
Identity = 132/241 (54.77%), Postives = 162/241 (67.22%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR*QN 3183
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KKK    R E +   +  T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+M PP K     ++  R +N
Sbjct:  567 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRN 791          

HSP 3 Score: 56.225 bits (134), Expect = 2.674e-7
Identity = 32/104 (30.77%), Postives = 51/104 (49.04%), Query Frame = 1
Query:   82 MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSK 393
            MT     +H +  +F+QLQ +   +   L+DV++ EF     LA+Y+PE  ID  G+  F+  +L    P      LF+SF  +          P+ +P +KSK
Sbjct:    1 MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLE-EFHGNGVLAKYNPEGTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKF---------PHSSPNVKSK 94          
BLAST of Diacylglycerol kinase vs. Ensembl Mouse
Match: Dgkb (diacylglycerol kinase, beta [Source:MGI Symbol;Acc:MGI:2442474])

HSP 1 Score: 504.212 bits (1297), Expect = 2.791e-163
Identity = 260/466 (55.79%), Postives = 308/466 (66.09%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVL-----------ERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E II QMM+VA YL WD  EL PIL +MM EIDYD DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+KK+  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL           ER+ TAK        K   S    + A D+                   Q +NS   +  QGL        QI  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  125 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTMPSAGASVPEERQSTAK--------KEKSSSQQPNKATDKNKM----------------QRANS-VTMDGQGL--------QITPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKF 555          

HSP 2 Score: 254.988 bits (650), Expect = 3.546e-71
Identity = 132/241 (54.77%), Postives = 162/241 (67.22%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR*QN 3183
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KKK    R E +   +  T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+M PP K     ++  R +N
Sbjct:  567 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRN 791          

HSP 3 Score: 56.225 bits (134), Expect = 2.674e-7
Identity = 32/104 (30.77%), Postives = 51/104 (49.04%), Query Frame = 1
Query:   82 MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSK 393
            MT     +H +  +F+QLQ +   +   L+DV++ EF     LA+Y+PE  ID  G+  F+  +L    P      LF+SF  +          P+ +P +KSK
Sbjct:    1 MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLE-EFHGNGVLAKYNPEGTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKF---------PHSSPNVKSK 94          
BLAST of Diacylglycerol kinase vs. Ensembl Mouse
Match: Dgka (diacylglycerol kinase, alpha [Source:MGI Symbol;Acc:MGI:102952])

HSP 1 Score: 414.075 bits (1063), Expect = 6.378e-130
Identity = 218/454 (48.02%), Postives = 277/454 (61.01%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENH-YPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRW 2247
            I + D+ CY +LLE G+PEDKLEF F+LYD D NG+LD +EVE II QMM VA YL+WD  EL PILQ+MM E+D D  G V+L+EW R G T +PLLVLLG+D  ++DDG H+W+ K F++  +C+LC +  +  G+QGL C FC Y VH+ C   A    + TY K++K   +  H W+    +  +C +C+K  +   + +G HC WC   IH  C+++    CD G  ++HILPP  IYPSVL   Q  K+       KT++  +L    A                                          F+I  + +T PLLVFIN KSGGKQG  ++ KFQYILNPRQV+DL KD GP  GL+FF+DV  FRIL+CGGDGTVGW+LE IDK  +    P VA+LPLGTGNDLARCLRWG GYEGE L KIL  I L  VV LDRW
Sbjct:   95 ICLSDVYCYFTLLEGGRPEDKLEFTFKLYDMDRNGILDSTEVEKIILQMMRVAEYLDWDVSELRPILQEMMREMDQDGSGSVSLDEWVRAGATTVPLLVLLGMDVTMKDDGNHIWRPKRFTRLVYCNLCEQS-ISLGKQGLSCNFCKYIVHDHCAMKAQPCEVSTYAKSRKDIGVQSHLWVRGGCHSGRCDRCQKKIRTYHSLTGLHCVWCHLEIHDDCLQAVGPECDCGLLRDHILPPCSIYPSVLVSGQECKH-------KTTDDTSLCTPEA------------------------------------------FRIEPVSNTHPLLVFINLKSGGKQGQSVLWKFQYILNPRQVFDL-KD-GPEPGLRFFKDVPQFRILVCGGDGTVGWVLETIDKANF-ATVPPVAVLPLGTGNDLARCLRWGRGYEGENLRKILKDIELSKVVYLDRW 495          

HSP 2 Score: 210.69 bits (535), Expect = 1.739e-56
Identity = 110/239 (46.03%), Postives = 143/239 (59.83%), Query Frame = 1
Query: 2467 SDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDI-ICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFEN---LATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFS 3171
            SD +P  IINNYFSIGVDASIAH+FH++REK+PEKFNSRM+N+ +Y E  TSE + +TCK L E + + IC  ++         EGIA+LNIPS +GG NLWG    P                D+        S      +   L T V DM D  LEVVG+E A+  G +    +S+G RLA+C E+  +T +  PMQIDGEPW+QA CTI IT+KNQ+PM+M PP   +     +S
Sbjct:  508 SDPVPSQIINNYFSIGVDASIAHRFHLMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESVTVEICGKLLDLSDLSL--EGIAVLNIPSTHGGSNLWGDTKRPH--------------GDTCEINQALGSAAKIITDPDILKTCVPDMSDKRLEVVGIEGAIEMGQIYTRLKSAGHRLAKCSEITFQTTKTLPMQIDGEPWMQAPCTIKITHKNQMPMLMGPPSNSYNFFGFWS 730          
BLAST of Diacylglycerol kinase vs. UniProt/SwissProt
Match: sp|Q03603|DGK3_CAEEL (Probable diacylglycerol kinase 3 OS=Caenorhabditis elegans OX=6239 GN=dgk-3 PE=3 SV=3)

HSP 1 Score: 669.848 bits (1727), Expect = 0.000e+0
Identity = 352/758 (46.44%), Postives = 466/758 (61.48%), Query Frame = 1
Query:  877 TDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRF-GRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKK 3147
            + + +I +K L+C LSLLE+  PE+KL+ +F +YD+DGNG LDKSE++ II QMMNVA Y  WDT EL  +++QMM +IDYDNDG V+ +EW+RGG+TNIPLLVLLG DT +++DG HVW+L+HF+K T+C+ C  ILV + G+QGL C  C YTVHERCV++A  +CI TY+ +++ D+L  HHW + +  AKC+KCK +  +G   G  CRWC   +H  CM +  Q CDLG    HILPP  I+P+ LERK +   K   N   +S S +L  + +   DC                                              +PLLV +NPKSGGKQG +I+ KF+Y+LNPRQVYDL K  GP  GLQ F  +K+  IL+CGGDGT+GW+LE++DK  + + RP VA+LPLGTGNDLARCLRWGGGYE E L KIL +I   +++ +DRWQI                                 + ++ S++  S           E+GD                P +IINNYFSIGVDASIAH+FHV+REK PEKFNSRMRN+ +Y E+GTSE L+++CK+LHE IDI+CDG  +DL + A LEGIA+LNIPS+YGG NLWG                 + +   GR       + +    L T VQD+GD L+E+VGLE+AM  G +  G R + +RL+QC  VVI+T + FPMQIDGEPW+Q  C I IT+KNQ  M++A   +K
Sbjct:  149 SQEPRIPLKPLICTLSLLEADTPENKLDVVFHVYDSDGNGFLDKSEIDGIIEQMMNVARYQQWDTIELEQVIRQMMVDIDYDNDGIVSFDEWRRGGLTNIPLLVLLGFDTEMKEDGSHVWRLRHFTKPTYCNACCSILVGWGGKQGLSCSLCKYTVHERCVRSAATNCIRTYS-SRQQDKLY-HHWQDANATAKCVKCKAT--VGVFQGKGCRWCHNYVHHRCMSALAQECDLGALVHHILPPTHIFPAFLERKTSTSLK---NHNFSSHSASLLQAVSPSNDC----------------------------------------------RPLLVLVNPKSGGKQGVKILQKFEYLLNPRQVYDLSK-TGPEPGLQLFSTLKNCNILVCGGDGTIGWVLESMDKMTFPHGRPPVAVLPLGTGNDLARCLRWGGGYENENLHKILEQIEKSSLIDMDRWQIKIE------------------------------ITENKSARRAS-----------EKGDTP--------------PYSIINNYFSIGVDASIAHRFHVMREKFPEKFNSRMRNKLWYFELGTSETLSSSCKNLHEQIDILCDGESIDLGQDASLEGIALLNIPSIYGGSNLWG-----------------RSRKSKGRMPGLFPMKNAEKMQLQTRVQDIGDGLIELVGLESAMQMGQIKAGVRGA-RRLSQCSTVVIQTHKSFPMQIDGEPWMQPPCIIQITHKNQAKMLVAAAPRK 779          
BLAST of Diacylglycerol kinase vs. UniProt/SwissProt
Match: sp|P49620|DGKG_RAT (Diacylglycerol kinase gamma OS=Rattus norvegicus OX=10116 GN=Dgkg PE=2 SV=1)

HSP 1 Score: 666.381 bits (1718), Expect = 0.000e+0
Identity = 356/773 (46.05%), Postives = 464/773 (60.03%), Query Frame = 1
Query:  865 NRPLTDQDK--IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNV-RDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFE--NLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKK--FGLR 3159
            N P +  D   + +KD++CYLSL+E+G+P+DKLEFMFRLYD+D N +LD++E++ I+SQM++VA YL WD  EL PIL++M+  +DY+ DG V+LEEW  GGMT IPLLVLLG+D +  + DG+H W LKHF K T+C+ CH +L+   +QGLCC++C Y VH+RCV N+   C+ TY+K K+S ++++H W+E +   KC +C KS K   + +  HC WC+ T H+ C  S    CD G+ K+HIL P  IYP   +R Q  K                +DS A                      ++  +  +Q           ++I   P T PLLV +NPKSGG+QG RI+ KF Y+LNP+QV++L K  GP  GL FF+D  DFR+L CGGDGTVGW+L+ IDK  +  + P VA+LPLGTGNDLARCLRWGGGYEG  L KIL +I    +VMLDRW +        PR E                                           E GD              ++P NI+NNYFSIGVDASIAH+FHV+REKHPEKFNSRM+N+ +Y E GTSE   ATCK LH++I++ CDGV +DL    +LEGIAILNIPS+YGG NLWG                   ++   R   RE SR+S  +   L   VQD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  V IRT++  PMQ+DGEPW+Q  C I IT+KNQ PM+M PP K   F LR
Sbjct:  145 NAPASSSDAPIVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENELLDQAELDQIVSQMLHVAQYLEWDPTELRPILKEMLQGMDYNKDGFVSLEEWVSGGMTTIPLLVLLGMDDSASKGDGRHAWTLKHFKKPTYCNFCHIMLMGVRKQGLCCIYCKYAVHQRCVSNSIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELS--TACDGGELKDHILLPTSIYPVTRDR-QAGK----------------SDSGA----------------------AAKGELVMQ-----------YKIIPSPGTHPLLVLVNPKSGGRQGERILQKFHYLLNPKQVFNLDKG-GPTPGLNFFQDTPDFRVLACGGDGTVGWILDCIDKANFTKH-PPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEV-----MPREE------------------------------------------VENGD--------------QVPYNIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDL-SNIFLEGIAILNIPSMYGGTNLWG-------------------ETKKNRAVIRE-SRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTKKLLPMQVDGEPWMQPPCMIKITHKNQAPMMMGPPQKSSFFSLR 781          

HSP 2 Score: 58.5362 bits (140), Expect = 3.173e-7
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 1
Query:  118 EDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRR 333
            E+F+QLQ +   +   ++DV+  EF  G  L QYDP KPI ++ +  F+ AYL  + PQ +   LFL+F ++
Sbjct:   12 EEFDQLQKYSEYSSKKIKDVL-AEFNEGGSLKQYDPHKPISYDVFKLFMRAYLEVDLPQPLSTNLFLAFSQK 82          
BLAST of Diacylglycerol kinase vs. UniProt/SwissProt
Match: sp|Q91WG7|DGKG_MOUSE (Diacylglycerol kinase gamma OS=Mus musculus OX=10090 GN=Dgkg PE=1 SV=1)

HSP 1 Score: 658.292 bits (1697), Expect = 0.000e+0
Identity = 349/767 (45.50%), Postives = 454/767 (59.19%), Query Frame = 1
Query:  877 TDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGL-DTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFE--NLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKK--FGLR 3159
            +D   + +KD++CYLSL+E+G+P+DKLEFMFRLYD+D NG+LD++E++ I+SQM++VA YL WD  EL PIL++M+  +DYD DG V+L+EW  GGMT IPLLVLLG+ D+  + DG+H W LKHF K T+C+ C  +L+  G+QGLCC++C YTVH+RCV      C+ T +K K+S ++++H W+E +   KC +C KS K   + +  HC WC+ T H+ C  S    CD G+ K+HIL P  I P   +R                                               Q   S   +  +G     ++ ++I   P T PLLV +NPKSGG+QG RI+ KF Y+LNP QV++L  + GP  GL FF D  DFR+L CGGDGTVGW+L+ IDK  +  + P VA+LPLGTGNDLARCLRWGGGYEG  L KIL +I    +VMLDRW +        PR E                                           E GD              ++P NI+NNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+ +Y E GTSE   ATCK LH++I++ CDGV +DL    +LEGIAILNIPS+YGG NLWG                   ++   R   RE SR+S  +   L   VQD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  V IRT +  PMQ+DGEPW+Q  CTI IT+KNQ PM+M PP K   F LR
Sbjct:  151 SDAPVVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENGLLDQAEMDQIVSQMLHVAQYLEWDPTELRPILKEMLQGMDYDKDGFVSLQEWINGGMTTIPLLVLLGMDDSGSKGDGRHAWTLKHFKKPTYCNFCRAMLMGVGKQGLCCIYCKYTVHQRCVSKTIHGCVKTNSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELS--TVCDGGELKDHILLPTSICPVSGDR-----------------------------------------------QGGKSDGSVAAKG---ELVTQYKIIPSPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPEQVFNL-DNGGPTPGLNFFHDTPDFRVLACGGDGTVGWILDCIDKANFTKH-PPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEV-----MPREE------------------------------------------VENGD--------------QVPYNIMNNYFSIGVDASIAHRFHMMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDL-SNIFLEGIAILNIPSMYGGTNLWG-------------------ETKKNRAVIRE-SRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTNKLLPMQVDGEPWMQPQCTIKITHKNQAPMMMGPPQKSSFFSLR 781          

HSP 2 Score: 58.9214 bits (141), Expect = 2.792e-7
Identity = 36/107 (33.64%), Postives = 54/107 (50.47%), Query Frame = 1
Query:  118 EDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRR-------HSNLEIYNCEPNKAPTLKSKINEDNGKR 417
            E+F+QLQ +   +   ++DV+  EF  G  L QYDP KPI ++ +  F+ AYL  + PQ +   LFL+F ++       H      + EPN      S  N DN  +
Sbjct:   12 EEFDQLQKYSEYSSKKIKDVL-AEFNEGGSLRQYDPHKPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRQETPDHPKEGASSSEPN-----VSDYNSDNAAK 112          
BLAST of Diacylglycerol kinase vs. UniProt/SwissProt
Match: sp|Q6NS52|DGKB_MOUSE (Diacylglycerol kinase beta OS=Mus musculus OX=10090 GN=Dgkb PE=1 SV=2)

HSP 1 Score: 504.212 bits (1297), Expect = 1.962e-162
Identity = 260/466 (55.79%), Postives = 308/466 (66.09%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVL-----------ERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E II QMM+VA YL WD  EL PIL +MM EIDYD DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+KK+  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL           ER+ TAK        K   S    + A D+                   Q +NS   +  QGL        QI  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  132 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTMPSAGASVPEERQSTAK--------KEKSSSQQPNKATDKNKM----------------QRANS-VTMDGQGL--------QITPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKF 562          

HSP 2 Score: 255.373 bits (651), Expect = 2.208e-70
Identity = 130/228 (57.02%), Postives = 157/228 (68.86%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KKK    R E +   +  T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+M PP K
Sbjct:  574 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPK 785          
BLAST of Diacylglycerol kinase vs. UniProt/SwissProt
Match: sp|Q9Y6T7|DGKB_HUMAN (Diacylglycerol kinase beta OS=Homo sapiens OX=9606 GN=DGKB PE=2 SV=2)

HSP 1 Score: 502.286 bits (1292), Expect = 1.404e-161
Identity = 252/455 (55.38%), Postives = 312/455 (68.57%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            I +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E IISQMM+VA YL WD  EL PIL +MM EIDYD+DG V+LEEW +GGMT IPLLVLLGL+ NV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+K++  ++ H+W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL+   T+     +++ +  +S    + +  +   +  +            Q +NS   +  QGL        Q+  +P T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY L  + GP  GL FFRDV DFR+L CGGDGTVGW+L+ I+K     + P VAILPLGTGNDLARCLRWGGGYEGE L KIL  I   T +MLDRW+ 
Sbjct:  133 IHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTLPTSG----VSVPEERQSTVKKEKSGSQQPNKVIDKNKM--------QRANS-VTVDGQGL--------QVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGN-GPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKH-PPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKF 564          

HSP 2 Score: 253.447 bits (646), Expect = 1.175e-69
Identity = 129/228 (56.58%), Postives = 158/228 (69.30%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTF---ENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK 3144
            D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+F+Y E GTSE  +ATCK LHE ++I CDGV +DL   + LEGIAILNIPS++GG NLWG                 KK+    R E +   + +T    + L  A QD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  VVIRT +  PMQIDGEPW+Q  CTI IT+KNQ PM+M PP K
Sbjct:  576 DPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINIS-LEGIAILNIPSMHGGSNLWG---------------ESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPK 787          
BLAST of Diacylglycerol kinase vs. TrEMBL
Match: V4A524 (Diacylglycerol kinase OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_164410 PE=3 SV=1)

HSP 1 Score: 801.971 bits (2070), Expect = 0.000e+0
Identity = 424/792 (53.54%), Postives = 527/792 (66.54%), Query Frame = 1
Query:  841 KSNNLDIVNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRES-----KTSNGCGRTG--CTGYCSRN-----RGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPP-LKKFGLRNMFSR 3174
            ++N+ DI N        I +KDL+CYLSLLE+G+PEDKLEFMFRLYD DGNG+LD SE++CI++QMM+VA YL WD  EL PILQ MM EIDYD+DG V++EEWK+GGMT IPLLVLLGLDTNV+DDG HVW+LKHFS+  +C+LC  +LV FG+QGL C FC YTVHERCV  APASCI TYTK+KK+ Q + HHW+E +   KC KC+KS K     +G HCRWC  T+H  C    +  CDLG  ++H LPPI I P+VL+R ++ KNK          S+   DSA                         N    +Q Q       SSFQ+     + PLLVFINPKSGGKQG RI+ KFQY+LNPRQVYD++K  GP  GLQFF+D+ + R+L+CGGDG+VGWLL+ IDK  +   +P +A+LPLGTGNDLARCLRWGGGYEGE L KIL +I     VM+DRW+I  S+P     +E         N     G    GY   N     + +    +   D    E S+ D  E+ +             D +P NIINNYFSIGVDASI H+FH++REKHPEKFNSRM+N+ +Y E GTSE L ATCKHLHE IDI+CDGV LDL  G  LEGIA+LNIPS+YGG NLWG   +PS+ K+ +  K KK+K     F +  +S       LA AVQD+GD L+EVVG++++MH G V  G R+SG+RLAQC +VVIRT++ FPMQIDGEPW+Q  CTI IT+KNQVPM++APP  KK  L  +F +
Sbjct:  251 RTNSFDIKN------GHIYLKDLVCYLSLLEAGRPEDKLEFMFRLYDADGNGLLDSSELDCIVNQMMSVAEYLGWDVSELKPILQDMMLEIDYDSDGVVSMEEWKKGGMTTIPLLVLLGLDTNVQDDGSHVWRLKHFSRPAYCNLCLNLLVGFGKQGLSCTFCKYTVHERCVHRAPASCITTYTKSKKTVQTMNHHWVEGNCAGKCDKCRKSIKSYNGVTGLHCRWCHLTLHNKCASQLKPECDLGPFRDHTLPPITICPAVLDRPRSDKNK----------SLTRVDSA-------------------------NLDGPMQGQ-------SSFQVIPFEGSHPLLVFINPKSGGKQGGRILRKFQYLLNPRQVYDMIK-CGPTEGLQFFKDIPNCRVLVCGGDGSVGWLLDTIDKMNF-AQKPPIAVLPLGTGNDLARCLRWGGGYEGENLIKILKRIEHSQAVMIDRWKIEFSKPDDAEDQEGDPIPYNIINNYFSIGVFAFGYEGENLIKILKRIEHSQAVMIDRWKIEFSKPDDAEDQE------------GDPIPYNIINNYFSIGVDASICHRFHIMREKHPEKFNSRMKNKLWYFEFGTSETLAATCKHLHEEIDIMCDGVSLDLSNGPRLEGIALLNIPSIYGGTNLWGD--NPSQKKRRKAQKAKKEKEKE--FSTSSMSS----AELAIAVQDVGDKLIEVVGIDSSMHVGQVYAGLRASGRRLAQCSQVVIRTRKRFPMQIDGEPWMQPPCTIYITHKNQVPMLVAPPSSKKSKLFKIFKK 972          
BLAST of Diacylglycerol kinase vs. TrEMBL
Match: A0A1D1URY8 (Diacylglycerol kinase OS=Ramazzottius varieornatus OX=947166 GN=RvY_01541-1 PE=3 SV=1)

HSP 1 Score: 792.727 bits (2046), Expect = 0.000e+0
Identity = 412/767 (53.72%), Postives = 506/767 (65.97%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDL-RKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFE----NLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPL-KKFGLRNMF 3168
            +I +KD++CYLSLLE G+PEDKLEFMFRLYD DGNGVLD  E+ECI++QMM VA YL WD  EL PIL++MM EIDYD DG V+L+EWKRGG+T IPLLVLLGLDTNV+DDG HVW+LKHF+K  +C+LC  +LV FG+QGL C FC YTVHERC   APA+CI TY K+KK  + + HHW+E + PAKC +CKKS K     +G HCRWC+ T+H  C    +  C LG+ ++HI+PP  + PSVLER+++      L  +K    M LA S +             +    + P SS +    Q +   +   +SFQ+  L  + PLLVFIN KSGGKQGARI+ KFQY+LNPRQVYDL ++ GPN GLQFF+DV +FRIL CGGDGTVGW+L+AIDK    N +P VA+LPLGTGNDLARCLRWGGGYEGE L+K+L K+    VV +DRW I  SEP                                S S +D            E GD               +P NIINNYFSIGVDASIAH+FHV+REKHPE+FNSRM+N+ +Y E GTSE  +ATCK LHE IDI+CDGV LDL  K   LEGIA+LNIPS+YGG NLWG         +   +K + K   S R +   + + S+      +L  A+QD+GD L+EVVG+E AMHAG V  G RSSG+RLAQC  VVIRT++ FPMQIDGEPW+Q  CTI IT+KNQVP++M PP   K    N F
Sbjct:  320 RISLKDIVCYLSLLEGGRPEDKLEFMFRLYDGDGNGVLDSHELECIVNQMMTVAEYLGWDVTELRPILREMMLEIDYDGDGSVSLDEWKRGGLTTIPLLVLLGLDTNVKDDGVHVWRLKHFNKPAYCNLCLNMLVGFGKQGLSCSFCKYTVHERCAARAPANCIATYVKSKKKAESMLHHWVEGNCPAKCSRCKKSIKTYNGITGLHCRWCQLTLHNKCASQVKPECTLGELRDHIVPPTAVCPSVLERQRSMP----LMPQK---KMGLAKSES-------------ALHGMVSPGSSQASEASQTEEAANGVPTSFQVTPLIGSHPLLVFINRKSGGKQGARILRKFQYLLNPRQVYDLGRN-GPNAGLQFFKDVPNFRILTCGGDGTVGWVLDAIDKLHL-NYKPPVAVLPLGTGNDLARCLRWGGGYEGESLNKVLKKLEKSQVVAMDRWHIEFSEP--------------------------------SESTDD------------EPGD--------------PIPYNIINNYFSIGVDASIAHRFHVMREKHPERFNSRMKNKLWYFEFGTSETFSATCKRLHEEIDIMCDGVSLDLANKYPPLEGIALLNIPSIYGGCNLWG---------EGAAHKKRVKSPGSSRKQKDWMEQGSSVSLSSMDLHFAIQDIGDKLIEVVGMEGAMHAGQVRAGLRSSGRRLAQCSSVVIRTRKRFPMQIDGEPWMQPPCTIHITHKNQVPVLMGPPTDSKMSFMNFF 997          
BLAST of Diacylglycerol kinase vs. TrEMBL
Match: A0A1W0WBN0 (Diacylglycerol kinase OS=Hypsibius dujardini OX=232323 GN=BV898_13145 PE=3 SV=1)

HSP 1 Score: 790.801 bits (2041), Expect = 0.000e+0
Identity = 405/763 (53.08%), Postives = 500/763 (65.53%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQI-SSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK-KFGLRNMF 3168
            ++ +KD++CYLSLLE G+PEDKLEFMFRLYD+DGNGVLD  E+E I++QMM VA YL WD  EL PIL++MM EIDYD DG V+L+EWKRGG+T IPLLVLLGLD NV+DDG HVW+LKHF+K  +C+LC  +LV FG+QGL C FC YTVHERC   AP +CI TY K+KK++Q++ HHW+E +YPAKC +C+K+ K     +G HCRWC+ T+H  C    +  C LG+ ++HILPPI I P+VLER+++       N      ++N ++SA   +                   S  SQ   +E+    N + +SFQ+   P + PLLVFIN KSGGKQGARI+ K QY+LNPRQVYDL +  GP  GLQFF+DV +FRIL CGGDGTVGW+L+AIDK    N +P +A+LPLGTGNDL RCLRWGGGYEGE L KIL K+    VV +DRWQI  S P                                S S +D            E GD               +P NIINNYFSIGVDASIAH+FH++REK+PE+FNSRM+N+ +Y E GTSE  +ATCK LHE IDI+CDGV LDL     LEGIA+LNIPS+YGG NLWG     S  KK     P  K+ D      +  S      +L  A+QD+GD L+EVVG+E AMHAG V  G RSSG+RLAQC  VVIRT++  PMQIDGEPW+Q  CTI IT+KNQVP++M PP   K    NMF
Sbjct:  424 RVSLKDIVCYLSLLEGGRPEDKLEFMFRLYDSDGNGVLDAHEMESIVNQMMTVAEYLGWDVTELRPILKEMMVEIDYDGDGSVSLDEWKRGGLTTIPLLVLLGLDANVKDDGVHVWRLKHFNKPAYCNLCLNMLVGFGKQGLSCSFCKYTVHERCAARAPTNCISTYVKSKKANQVMMHHWVEGNYPAKCTRCRKNVKTYNGITGLHCRWCQMTLHNKCASQVKPECTLGELRDHILPPIAICPAVLERQRSVPLTQ--NQSNRKLAINRSESALQGLTSPG--------------SSQGSQGSREEETTQVNGVPTSFQVTPFPGSHPLLVFINRKSGGKQGARILRKLQYLLNPRQVYDLGRS-GPGLGLQFFKDVPNFRILCCGGDGTVGWVLDAIDKLHL-NYKPPLAVLPLGTGNDLGRCLRWGGGYEGESLFKILKKVEKSQVVAMDRWQIEFSPP--------------------------------SDSTDD------------EPGD--------------PIPYNIINNYFSIGVDASIAHRFHLMREKYPERFNSRMKNKLWYFEFGTSETFSATCKRLHEEIDIMCDGVALDLANRPPLEGIALLNIPSIYGGCNLWG---EGSTNKKRVKKSPGTKRKDDKWSTDQGSSVSLASMDLHFAMQDIGDKLIEVVGMEGAMHAGQVRAGLRSSGRRLAQCSSVVIRTRKRLPMQIDGEPWMQPPCTIHITHKNQVPVLMGPPPSHKMSFMNMF 1107          
BLAST of Diacylglycerol kinase vs. TrEMBL
Match: A0A1W0WBI2 (Diacylglycerol kinase OS=Hypsibius dujardini OX=232323 GN=BV898_13145 PE=3 SV=1)

HSP 1 Score: 788.875 bits (2036), Expect = 0.000e+0
Identity = 405/763 (53.08%), Postives = 500/763 (65.53%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQI-SSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLK-KFGLRNMF 3168
            ++ +KD++CYLSLLE G+PEDKLEFMFRLYD+DGNGVLD  E+E I++QMM VA YL WD  EL PIL++MM EIDYD DG V+L+EWKRGG+T IPLLVLLGLD NV+DDG HVW+LKHF+K  +C+LC  +LV FG+QGL C FC YTVHERC   AP +CI TY K+KK++Q++ HHW+E +YPAKC +C+K+ K     +G HCRWC+ T+H  C    +  C LG+ ++HILPPI I P+VLER+++       N      ++N ++SA                    L    +SQ   +E+    N + +SFQ+   P + PLLVFIN KSGGKQGARI+ K QY+LNPRQVYDL +  GP  GLQFF+DV +FRIL CGGDGTVGW+L+AIDK    N +P +A+LPLGTGNDL RCLRWGGGYEGE L KIL K+    VV +DRWQI  S P                                S S +D            E GD               +P NIINNYFSIGVDASIAH+FH++REK+PE+FNSRM+N+ +Y E GTSE  +ATCK LHE IDI+CDGV LDL     LEGIA+LNIPS+YGG NLWG     S  KK     P  K+ D      +  S      +L  A+QD+GD L+EVVG+E AMHAG V  G RSSG+RLAQC  VVIRT++  PMQIDGEPW+Q  CTI IT+KNQVP++M PP   K    NMF
Sbjct:  424 RVSLKDIVCYLSLLEGGRPEDKLEFMFRLYDSDGNGVLDAHEMESIVNQMMTVAEYLGWDVTELRPILKEMMVEIDYDGDGSVSLDEWKRGGLTTIPLLVLLGLDANVKDDGVHVWRLKHFNKPAYCNLCLNMLVGFGKQGLSCSFCKYTVHERCAARAPTNCISTYVKSKKANQVMMHHWVEGNYPAKCTRCRKNVKTYNGITGLHCRWCQMTLHNKCASQVKPECTLGELRDHILPPIAICPAVLERQRSVPLTQ--NQSNRKLAINRSESALQG-----------------LTSPGSSQGSREEETTQVNGVPTSFQVTPFPGSHPLLVFINRKSGGKQGARILRKLQYLLNPRQVYDLGRS-GPGLGLQFFKDVPNFRILCCGGDGTVGWVLDAIDKLHL-NYKPPLAVLPLGTGNDLGRCLRWGGGYEGESLFKILKKVEKSQVVAMDRWQIEFSPP--------------------------------SDSTDD------------EPGD--------------PIPYNIINNYFSIGVDASIAHRFHLMREKYPERFNSRMKNKLWYFEFGTSETFSATCKRLHEEIDIMCDGVALDLANRPPLEGIALLNIPSIYGGCNLWG---EGSTNKKRVKKSPGTKRKDDKWSTDQGSSVSLASMDLHFAMQDIGDKLIEVVGMEGAMHAGQVRAGLRSSGRRLAQCSSVVIRTRKRLPMQIDGEPWMQPPCTIHITHKNQVPVLMGPPPSHKMSFMNMF 1104          
BLAST of Diacylglycerol kinase vs. TrEMBL
Match: R7TZB0 (Diacylglycerol kinase OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_91694 PE=3 SV=1)

HSP 1 Score: 783.867 bits (2023), Expect = 0.000e+0
Identity = 417/764 (54.58%), Postives = 506/764 (66.23%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPP-LKKFGLRNMFSR 3174
            +I +KDL+CYLSLLE+G+PEDKLEFMFR+YDTDGNG+LD SE+E I++QMM VA YL WD  EL PILQ MM EIDYD DG V+LEEWKRGG+T IPLLVLLGL+ NV DDG H W+LKHFSK  +C+LC  +LV  G+QGL C FC YTVHERCVQ APA CI TY K+KK+   + HHW+E + P KC +CKKS K     SG HCRWCK T+H  C    +  CD G+ KEHILPP  I P+VL    +  +  C N+ + S+S   + S  ++V               ++     + C     G    Q SSFQI  +  T PLL+FINPKSGGKQGARIM KFQ+ILNPRQVYD++K  GP  GLQF++ +  FR+L CGGDGTVGWLLEA+DK  +   +P VA+LPLGTGNDLARCLRWGGGYEGE L K L KI    VVM+DRWQI                                    + SS  D+G          E+G              D +P NIINNYFSIGVDASIAH+FH++REKHPEKFNSRMRN+ +Y EVGT+E L A CK+LHE IDI+CDG  LDL  G  LEGIA+LNIPS+YGG NLWG   +P++ K+ +  K  KK+ +     S   S + +F     A+QD+GD  +EVVGLEN++HA  +  G RSSG+R+AQC  VVIRT+  FPMQIDGEPW+Q  CTISIT+KNQVPM+MAPP  KK  L N+F R
Sbjct:  133 RIAIKDLVCYLSLLEAGRPEDKLEFMFRIYDTDGNGILDSSELEAIVNQMMAVAEYLGWDVSELKPILQDMMIEIDYDADGAVSLEEWKRGGLTTIPLLVLLGLEPNVTDDGTHTWRLKHFSKPAYCNLCLNLLVGLGKQGLSCSFCKYTVHERCVQRAPACCITTYVKSKKTSISMNHHWVEGNCPGKCDRCKKSIKTYNGVSGLHCRWCKITLHNKCASQVKPECDFGEFKEHILPPTAICPAVLNMSHS--DSSCDNLAQLSDS---SSSQPEKV--------------MVVFGVRYNYCHTNNGG----QSSSFQITPVDGTIPLLIFINPKSGGKQGARIMRKFQFILNPRQVYDMLKG-GPAEGLQFYKQLPQFRVLCCGGDGTVGWLLEAMDKMNF-VEKPPVAVLPLGTGNDLARCLRWGGGYEGENLHKYLHKISRSVVVMMDRWQI------------------------------------EFSSTEDTG----------EQG--------------DPIPYNIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMRNKIWYFEVGTAETLGARCKNLHENIDIMCDGCSLDLANGPSLEGIAVLNIPSMYGGSNLWGE--TPTQKKRKKAKKNGKKEKEREYSSSSMSSNDLSF-----AIQDVGDKQIEVVGLENSLHAAGIKVGIRSSGRRMAQCSSVVIRTRCRFPMQIDGEPWMQPPCTISITHKNQVPMLMAPPSSKKATLFNLFKR 804          

HSP 2 Score: 65.4698 bits (158), Expect = 7.390e-7
Identity = 40/134 (29.85%), Postives = 74/134 (55.22%), Query Frame = 1
Query:  121 DFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSKINEDNGKRI----IYESRIRNNSLNATSTNAILSNLSERLSAI 510
            +F+QLQ++ S +   L+DV++ EF     L++Y+PE+PID+ G+ QF+  YL  + P  +   LF+SFMRR + +   +    ++ +   K+N +N K      +  S +R  S++  +    + +L   LS +
Sbjct:   15 EFHQLQEYQSYSSKKLKDVLE-EFHGDGCLSRYNPEEPIDYEGFLQFMEHYLEADLPSELTHNLFMSFMRRPA-MSASDPWLRQSSSSLMKLNSENSKSQTNPNLASSAVRRESIDIATGRIAIKDLVCYLSLL 146          
BLAST of Diacylglycerol kinase vs. Ensembl Cavefish
Match: dgkaa (diacylglycerol kinase, alpha a [Source:ZFIN;Acc:ZDB-GENE-060616-305])

HSP 1 Score: 585.489 bits (1508), Expect = 0.000e+0
Identity = 333/761 (43.76%), Postives = 427/761 (56.11%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIE-NHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNM 3165
            ++ +KD+ CY SLLE G+P DKLEF FRLYD DGNGVLD SEV+ II+QMM+ A YL WD  EL P+L+ MM  ID D+ G V+L+EW  GGM N+PLLVLLGL    + DG+H+W++KHF+K  +C++C  +L+   +QGLCC  C YTVH RC    P  C  TY  +KK   +  H W+  N    KC +C+K  K L   +G  C WC    H  C+      C+ G  ++HILPP  IYP + ER    KN  C  +   ++     D    +V                  QS  + C      +  N  +   +                 GGKQG R++ KFQY+LNPRQVY+L    GP  GL FFRD++D+RIL+CGGDGTVGW+L+AIDK      RP VA+LPLGTGNDLARCLRWGGGY+G  L +IL  I   T V +DRW +                                             + EDSQ    E GD               +P  IINNYFSIGVDASIAH+FH +REKHP+KFNSRM+N+ +Y E  TSE ++A+CK L E + I C GV LDL   + LEGIA+LNIPS++GG NLWG                + KKSD G  +  E+  +   E L  + QD+ D  LEVVGLE AM  G +  G +S+  RLA+  ++ IRT++  PMQIDGEPW+Q  CTI IT+KNQ  M+MAP  K  G  N+
Sbjct:   96 EVFLKDVSCYFSLLEDGQPRDKLEFAFRLYDRDGNGVLDSSEVDRIIAQMMHAADYLGWDVSELRPVLKDMMTAIDSDSSGTVSLDEWVEGGMNNVPLLVLLGLKMTQK-DGQHLWRMKHFNKPVYCNVCQNMLLGLRKQGLCCTCCKYTVHGRCANRNPDPCTRTYVMSKKDIGVSTHDWVSGNCDSGKCDRCQKKIKSLHGLTGKRCVWCHTLRHDECVNQEPSECNCGPLRDHILPPWAIYPVIKERTNCVKNG-CSGVTDDTDHNTTPDGQVLQVG-----------------QSLWNPCNYTYSVIPCNTTAGMDV---------------SDGGKQGERVLRKFQYLLNPRQVYNL-SSGGPGPGLCFFRDLQDYRILVCGGDGTVGWILDAIDKASL-PVRPPVAVLPLGTGNDLARCLRWGGGYDGVDLGRILKDIEFSTPVQMDRWSVEV-------------------------------------------VLEDSQ----ERGD--------------PVPYEIINNYFSIGVDASIAHRFHTMREKHPQKFNSRMKNKLWYFEFATSETISASCKKLKECLTIECCGVQLDLSNLS-LEGIAVLNIPSMHGGSNLWG----------------EAKKSDRGGQDLPEVIVDP--EELKVSPQDLSDKRLEVVGLEGAMEMGQIYTGLKSA-VRLAKTSQITIRTKKPLPMQIDGEPWMQPPCTIHITHKNQASMLMAPQSKSSGFFNL 739          
BLAST of Diacylglycerol kinase vs. Ensembl Cavefish
Match: dgkab (diacylglycerol kinase beta [Source:NCBI gene;Acc:103047572])

HSP 1 Score: 384.03 bits (985), Expect = 1.210e-118
Identity = 206/453 (45.47%), Postives = 271/453 (59.82%), Query Frame = 1
Query:  898 MKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHY-PAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            +KD+ CY S+LE G P DKLEF F+LYD +GNG+L+ SEV+ II+QMM  A YL WD  EL P L++MM  ID D++G VT EEW +GGM N+PLLVLLGL      DG+HVW++KHF++ T+C +C  +L+  G+QGL C  C  TVH  C     + C  T+  +K+   L  H W+      +KC  C K  K+ A +G  C WC  T H  C+      CD G  K+H+LPP  I    + +K    +  CL      E  +LA+  +D                                          QI  +  + PLLVF+NPKSGGKQG R++ KFQY+LNPRQVY+L  + GPN GL FFR+++ ++IL+CGGDGTVGW+L+AIDK +   N P VAILPLGTGNDLARCLRWGGGYEG  L +IL +I    V+ +DRW++
Sbjct:  106 IKDVSCYFSVLEDGNPRDKLEFTFKLYDQNGNGLLESSEVDKIITQMMRAAEYLGWDVTELKPALKEMMAAIDADSNGTVTQEEWIQGGMNNVPLLVLLGLKM-ADKDGQHVWRMKHFNRPTYCFVCQNMLLGLGKQGLSCNVCKCTVHSHCANKYHSPCAATFITSKEEIGLAVHAWVSADCDSSKCEICHK--KIKALAGKRCVWCHLTRHNECVSVGSVNCDCGPLKDHVLPPWAI--CSVNKKDGGSH--CL------EESDLANITSDG--------------------------------------HFLQIFPVHYSNPLLVFVNPKSGGKQGGRVLRKFQYLLNPRQVYNL-SNGGPNPGLHFFRNLQKYKILVCGGDGTVGWVLDAIDKADMAIN-PPVAILPLGTGNDLARCLRWGGGYEGSDLSEILKEIEESVVMKMDRWKV 505          

HSP 2 Score: 223.016 bits (567), Expect = 9.052e-61
Identity = 116/241 (48.13%), Postives = 155/241 (64.32%), Query Frame = 1
Query: 2443 VLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNM 3165
            V ++++   D +P  IINNYFSIGVDASIAH+FH +REKHP+KFNSR +N+ +Y E+ TSE ++A+CK+L E + I C GV LDL   + LEGIA+LNIPS++GG NLWG           +  K +    D G         +     L  + QDM D LLEVVGLE A+  G +  G +S G RLAQ  +++I+T++  PMQIDGEPW+Q  CTI IT+KNQ  M+ APP K  G  ++
Sbjct:  508 VPNDENEVGDPLPSEIINNYFSIGVDASIAHRFHTMREKHPQKFNSRAKNKLWYFELATSETISASCKNLRECLTIQCCGVRLDLSSHS-LEGIAVLNIPSMHGGSNLWG-----------ETKKTEDVYKDGGHCPQVITDTDV----LERSSQDMSDKLLEVVGLEGAIEMGQIYTGLKSKGHRLAQTTQIMIKTKKALPMQIDGEPWMQPPCTIHITHKNQASMLAAPPAKSSGFFHL 732          
BLAST of Diacylglycerol kinase vs. Ensembl Cavefish
Match: dgkg (diacylglycerol kinase, gamma [Source:ZFIN;Acc:ZDB-GENE-081105-39])

HSP 1 Score: 309.686 bits (792), Expect = 1.780e-94
Identity = 143/259 (55.21%), Postives = 185/259 (71.43%), Query Frame = 1
Query:  874 LTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAK 1647
            L+    + +KD++CYLSLLE G PEDKLEFMFRLYDTDGNG+LD SE++ II+QM++VA YL WD+ EL PIL++MM EIDYD DG VTLEEW RGGMT IPLLVLLG++TNV +DG+HVW+LKHF+K  +C+ CH +L+   +QGLCC  C YTVHERCV    A+CI TY K+++    ++H WIE + P KC +C +S K     +G HC WC+ T+H  C  + +  CD G  ++H L P  I P VL+R    K
Sbjct:  149 LSSPQVVFLKDIVCYLSLLERGTPEDKLEFMFRLYDTDGNGLLDSSELDRIINQMVHVAEYLEWDSTELRPILKEMMEEIDYDRDGTVTLEEWIRGGMTTIPLLVLLGMETNVEEDGQHVWRLKHFNKPAYCNYCHTMLLGVRKQGLCCSLCKYTVHERCVSKDIAACISTYAKSRRHTNAMQHVWIEGNSPTKCDRCHRSIKYYQGLTGLHCVWCQITVHNKCASNVKAECDGGPLRDHTLLPSYICPVVLDRHSVVK 407          
BLAST of Diacylglycerol kinase vs. Ensembl Cavefish
Match: dgkzb (diacylglycerol kinase zeta [Source:NCBI gene;Acc:103036104])

HSP 1 Score: 287.73 bits (735), Expect = 2.265e-81
Identity = 209/666 (31.38%), Postives = 293/666 (43.99%), Query Frame = 1
Query: 1222 GKHVWKLKHFSK------STHC--SLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPA---SCIHTY----TKTKKSDQLLEHHWIENHYP-AKCIKCKKSTKLGAKSGFH--------CRWCKRTIHK---CCM-ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPD--TKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            G+HVW     S        +HC      K L R+      C  C  +VH  C++        C  ++    T+  +    + HHW+       KC +C K      K  FH        C WCK+  H    C M +   +TC LG +   I+PP  I   +  R+Q A  K     +KTS    L +  +                               ++G    +   F +  +P    KPLLVF+NPKSGG QG++I+  F + LNPRQV+DL +  GP +GL+ +R V + RIL+CGGDGTVGW+L  +D  + N  +P+VA+LPLGTGNDLAR L WG GY  E + KIL+ +  G +V LDRW ++  EP P    + K                                                     D   +D++P N+ NNYFS+G DA +  +FH  RE  PEKFNSR+RN+ FY     S+ L  + K L ++I ++CDG+ L  + +   L+ +  LNIP    G   WG                    S+   FE                 Q   D  +EV+G      A L  GG    G+RL QCREV++ T +  P+Q+DGEP   A   I IT +NQ  M+
Sbjct:  107 GQHVWFETSLSGDFCYVGESHCFAKSLQKSLPRYK-----CAACKISVHTTCMEQLEKINFRCKPSFREPGTRNVRETTSVRHHWVHRRRQDGKCRQCGKG--FQQKFSFHNKDIVAISCSWCKQAYHNKVTCFMLQQIEETCSLGAHAAVIVPPSWI---IRVRRQQASLKSSKRRKKTS----LKNKGS-------------------------------KKGAEDGKWKHFIVKPIPSQLMKPLLVFVNPKSGGNQGSKIIRSFMWFLNPRQVFDLSQG-GPKDGLEMYRKVPNLRILVCGGDGTVGWILSVLDDLQLNP-QPAVAVLPLGTGNDLARTLNWGAGYTDEPVSKILSHVEDGNIVQLDRWNLSV-EPNPEATEDEK-----------------------------------------------------DEHQTDKLPINVFNNYFSLGFDAHVTLEFHESREAKPEKFNSRLRNKMFYAGTAFSDFLMRSSKDLSKHIRVVCDGMDLTSKVQELKLQCLLFLNIPRYCAGTTPWG------------------NPSEHHDFEP----------------QRHDDGCIEVIGFTMTSLATLQVGGH---GERLHQCREVMLTTFKPIPVQVDGEPCRLAPSIIRITLRNQANMV 634          
BLAST of Diacylglycerol kinase vs. Ensembl Cavefish
Match: dgkzb (diacylglycerol kinase zeta [Source:NCBI gene;Acc:103036104])

HSP 1 Score: 288.886 bits (738), Expect = 1.198e-80
Identity = 209/666 (31.38%), Postives = 293/666 (43.99%), Query Frame = 1
Query: 1222 GKHVWKLKHFSK------STHC--SLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPA---SCIHTY----TKTKKSDQLLEHHWIENHYP-AKCIKCKKSTKLGAKSGFH--------CRWCKRTIHK---CCM-ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPD--TKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            G+HVW     S        +HC      K L R+      C  C  +VH  C++        C  ++    T+  +    + HHW+       KC +C K      K  FH        C WCK+  H    C M +   +TC LG +   I+PP  I   +  R+Q A  K     +KTS    L +  +                               ++G    +   F +  +P    KPLLVF+NPKSGG QG++I+  F + LNPRQV+DL +  GP +GL+ +R V + RIL+CGGDGTVGW+L  +D  + N  +P+VA+LPLGTGNDLAR L WG GY  E + KIL+ +  G +V LDRW ++  EP P    + K                                                     D   +D++P N+ NNYFS+G DA +  +FH  RE  PEKFNSR+RN+ FY     S+ L  + K L ++I ++CDG+ L  + +   L+ +  LNIP    G   WG                    S+   FE                 Q   D  +EV+G      A L  GG    G+RL QCREV++ T +  P+Q+DGEP   A   I IT +NQ  M+
Sbjct:  354 GQHVWFETSLSGDFCYVGESHCFAKSLQKSLPRYK-----CAACKISVHTTCMEQLEKINFRCKPSFREPGTRNVRETTSVRHHWVHRRRQDGKCRQCGKG--FQQKFSFHNKDIVAISCSWCKQAYHNKVTCFMLQQIEETCSLGAHAAVIVPPSWI---IRVRRQQASLKSSKRRKKTS----LKNKGS-------------------------------KKGAEDGKWKHFIVKPIPSQLMKPLLVFVNPKSGGNQGSKIIRSFMWFLNPRQVFDLSQG-GPKDGLEMYRKVPNLRILVCGGDGTVGWILSVLDDLQLNP-QPAVAVLPLGTGNDLARTLNWGAGYTDEPVSKILSHVEDGNIVQLDRWNLSV-EPNPEATEDEK-----------------------------------------------------DEHQTDKLPINVFNNYFSLGFDAHVTLEFHESREAKPEKFNSRLRNKMFYAGTAFSDFLMRSSKDLSKHIRVVCDGMDLTSKVQELKLQCLLFLNIPRYCAGTTPWG------------------NPSEHHDFEP----------------QRHDDGCIEVIGFTMTSLATLQVGGH---GERLHQCREVMLTTFKPIPVQVDGEPCRLAPSIIRITLRNQANMV 881          
BLAST of Diacylglycerol kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002929.1 (pep scaffold:Pmarinus_7.0:GL476935:158222:277059:-1 gene:ENSPMAG00000002642.1 transcript:ENSPMAT00000002929.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 484.952 bits (1247), Expect = 2.463e-158
Identity = 247/455 (54.29%), Postives = 299/455 (65.71%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            + +KD++CYLSLLE  +PEDKLEFMF LYDTD N +LD SEV+ II+QMM+VA YL WD  EL PIL+ MM EIDYD DG V+LEEW +GGMT IPLLVLLGL++NV+DDG+HVWKLKHF+K  +C+ C  +L+  G+QGLCC FC YTVHERCV  AP +CI TY K+KKS  +++H+W+E + PAKC KC KS K     +G HC WC  T+H  C    +  CD G  K+H+LPP  I+P VL       N  C   E+          +   +D                 Q +NS       G  H    + QI     T PLLVF+NPKSGGKQG +I+ KFQY+LNPRQVY+L K  GP  GL FFRDV DFR+L CGGDGTVGW+L+ IDK      RP VA+LPLGTGNDLARCL WGGGYEG  L K L  +   T +MLDRW I
Sbjct:    3 VHLKDIVCYLSLLEGSRPEDKLEFMFHLYDTDSNKLLDSSEVDRIITQMMHVAEYLEWDVTELKPILRDMMQEIDYDQDGTVSLEEWIQGGMTTIPLLVLLGLESNVKDDGQHVWKLKHFNKPAYCNFCKAMLIGLGKQGLCCTFCKYTVHERCVAKAPLNCISTYAKSKKSATVMDHYWVEGNCPAKCDKCHKSIKCYQGLTGLHCVWCHITLHNKCASHVKPECDCGQLKDHVLPPNTIFPLVLIMGIPTPNA-CEPQERQMSVQRTDSGSGASLDDDVLRRF----------QRTNSV-----TGDGH----ALQITPTTGTHPLLVFVNPKSGGKQGEKILRKFQYLLNPRQVYNLNKG-GPFPGLNFFRDVPDFRVLTCGGDGTVGWVLDCIDKLSL-PKRPPVAVLPLGTGNDLARCLHWGGGYEGGSLRKTLKDVENSTPLMLDRWSI 435          

HSP 2 Score: 238.81 bits (608), Expect = 2.503e-67
Identity = 122/239 (51.05%), Postives = 161/239 (67.36%), Query Frame = 1
Query: 2467 SDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR*QN 3183
             D +P +IINNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+ +Y E GTSE L ATCK L+E++++ CDGV LDL   + LEGI +LNIPS++GG NLWG   +    +++ + +  ++K  +   + REL             QD+ D+LLEVVGL+ AM  G +  G +S+G RLAQC  V IRT +  PMQIDGEPW+Q  CTI I +KNQ PM+M P   K G  +   R +N
Sbjct:  446 GDPVPYSIINNYFSIGVDASIAHRFHLMREKHPEKFNSRMKNKLWYFEFGTSETLFATCKKLYEFMEVECDGVQLDLANSS-LEGITVLNIPSMHGGSNLWGETNTRRVSRRSSMRRSGREKVQATIADQRELK---------FCQQDLSDHLLEVVGLDGAMEMGQIYTGLKSAGSRLAQCSSVTIRTTKLLPMQIDGEPWMQPPCTIKIIHKNQAPMLMGPA-PKTGFFSFLRRKKN 673          
BLAST of Diacylglycerol kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009725.1 (pep scaffold:Pmarinus_7.0:GL478043:6842:50807:-1 gene:ENSPMAG00000008779.1 transcript:ENSPMAT00000009725.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 476.863 bits (1226), Expect = 1.542e-155
Identity = 244/454 (53.74%), Postives = 299/454 (65.86%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKK-SDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRW 2247
            + +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG+LD SE++ II+QMM+VA YL WD  EL PILQ+MM EIDYD DG V+L+EW RGG+T IPLLVLLGL+TNV+DDG+HVW+LKHF+K  +C+LC  +LV  G+QGLCC FC YTVHERCV  AP SCI TY K+KK +++++ H W+E +   KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+H+LPP  I P VL           +   KT+ S         ++ C                  S + C +             QI  +  T PLLVF NPKSGGKQG RI+ K QY+LNPRQVY+L K  GP  GL FFRDV DFR+L CGGDGTVGW+L+ I         P VA+LPLGTGNDLARCLRWGGGYEG  L K++  I   +VVMLDRW
Sbjct:    2 VFLKDIVCYLSLLEGGRPEDKLEFMFRLYDTDGNGLLDHSELDRIITQMMHVAEYLEWDVTELKPILQEMMEEIDYDQDGTVSLDEWIRGGLTTIPLLVLLGLETNVKDDGQHVWRLKHFNKPAYCNLCLNMLVGLGKQGLCCSFCKYTVHERCVARAPLSCITTYVKSKKDANKVMHHFWVEGNCHTKCHKCHKAIKCYQGLTGLHCAWCQITLHNKCASHVKPECDCGPLKDHVLPPSSICPIVL-----------VACRKTALSRRCHKRTKKKLHC-----------------GSATVCLLS------------QITPVLGTHPLLVFGNPKSGGKQGDRILRKIQYLLNPRQVYNLAKG-GPMPGLSFFRDVPDFRVLACGGDGTVGWVLDCIADKAGLTRHPPVAVLPLGTGNDLARCLRWGGGYEGGSLMKVMKDIENSSVVMLDRW 414          

HSP 2 Score: 209.149 bits (531), Expect = 2.516e-57
Identity = 106/224 (47.32%), Postives = 140/224 (62.50%), Query Frame = 1
Query: 2467 SDEMPCNIINNYFSIGVDASIAHKFHVLREKH-PEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
             D +P +IINNYFS+GV   I H  H +  K+ P      M+N+ +Y E GTSE  +ATCK LHE I+I CDG++LDL   + LEG+A+LNIPS++GG NLWG              +   ++    R   R ++  +  + L   VQD+GD LLEVVGLE AM  G +  G +S+GKR+AQC  V IRT +  PMQIDGEPW+Q  CTI +T+KNQ PM+M P
Sbjct:  427 GDPVPYSIINNYFSMGVHLPIQHGVHFIATKYFPPLCEGSMKNKLWYFEFGTSETFSATCKKLHECIEIECDGMLLDLSNTS-LEGVALLNIPSMHGGSNLWG----------ESRRRRNYRRQSKKRPPERAMTTVTDPKELKYCVQDLGDQLLEVVGLEGAMEMGQIYTGLKSAGKRMAQCSSVTIRTTKLLPMQIDGEPWMQPPCTIKVTHKNQAPMLMGP 639          
BLAST of Diacylglycerol kinase vs. Ensembl Sea Lamprey
Match: dgkzb (diacylglycerol kinase, zeta b [Source:ZFIN;Acc:ZDB-GENE-111104-4])

HSP 1 Score: 268.855 bits (686), Expect = 4.388e-79
Identity = 208/660 (31.52%), Postives = 279/660 (42.27%), Query Frame = 1
Query: 1222 GKHVWKLKHFSKSTHCSL----CHKILVRFGRQGLCCVFCLYTVHERCV---QNAPASCIHTY----TKTKKSDQLLEHHWIENH-YPAKCIKCKKSTKLGAKSGFH--------CRWCKRTIH---KCCM-ESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            G HVW     S    C L    C K + R       C  C   VH  C+   +     C  T+    T++ +    + HHW+  H    +C +C K  +   K  FH        C WCK+  H    C M +   + C LG +   I+PP  I    ++R Q   +K      K SE       A   V                                           A P  KPL+VFINPKSGG QG +IM    ++LNPRQV+DL    GP   L+ +R V + R+L CGGDGTVGW+L  +D+ +     P VAILPLGTGNDLAR L WGGGY  E + K+L+ +  G  V LDRW +       RP                     N  V +D   Q                        S+ S   +++P ++ NNYFS+G DA +  +FH  RE +PEKFNSR RN+ FY     S+ L  + + L +++ ++CDGV L  + +   L+ I  LNIP    G   WG                    +D   FE                 Q   D  +EV+G   A  A L  GG    G+RL QCRE V+ T R  P+Q+DGEP   A   I I+ +NQ  M+
Sbjct:   24 GDHVWFDSGDSGDGCCYLGEDGCVKSMSRKK-----CASCRIVVHVACIPQLEQINFRCKPTFREAGTRSAREASAVRHHWVHRHRQEGRCRQCGKGFQ--QKFTFHSKEIVAISCSWCKQAYHNKVSCFMLQQIEEPCSLGVHAAVIVPPTWII--RVKRPQRKSSK------KGSEECKWRPFAVKAV-------------------------------------------ASPLVKPLVVFINPKSGGNQGNKIMQSLMWLLNPRQVFDL-GAGGPQEALELYRKVPNLRVLACGGDGTVGWILSVLDQLQIVPP-PPVAILPLGTGNDLARTLNWGGGYTDEPISKVLSHVEEGATVQLDRWNLDV-----RP---------------------NPDVGRDEQEQGT----------------------SKPSPNPNQLPLDVFNNYFSLGFDARVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLAKHVRVMCDGVDLTPKIQELKLQCIVFLNIPRYCAGTVPWG------------------NPADHHDFEP----------------QRHDDGYIEVIGFTMASLAALQVGGH---GERLHQCREAVLATLRTTPVQVDGEPCRLAPSIIRISLRNQALMV 538          
BLAST of Diacylglycerol kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007078.1 (pep scaffold:Pmarinus_7.0:GL477921:24257:38296:1 gene:ENSPMAG00000006363.1 transcript:ENSPMAT00000007078.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 228.409 bits (581), Expect = 2.964e-67
Identity = 152/413 (36.80%), Postives = 201/413 (48.67%), Query Frame = 1
Query: 1867 PLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAID--KYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISI 3099
            PLLV INP+SGG QG  ++  F+  +NP QV+DL    GP  GL  FR+V  FR+L+CGGDGTVGW+L  ++  K +    +P VAILPLGTGNDLAR LRWG GY GE    +L  +     V +DRW I      P                    C++ R     S                           +   +Y  ++ C  +NNYF IGVDA ++  FH  RE+ P KFNSR+ N+  Y++VG  ++     ++LH  I +  D V +DL     +EG+  LNIPS   G +LWG                    SDS          E  F         M D LLEVVG+   MH G V GG R SG R+AQ + + +  QR  P+Q+DGEPW+Q    I +
Sbjct:   11 PLLVLINPRSGGMQGRELLHSFRKAVNPHQVFDLAC-GGPLPGLHVFREVPRFRLLVCGGDGTVGWVLSLLEDIKLKLACTQPPVAILPLGTGNDLARVLRWGPGYSGEDPLSVLINVDEAQEVKMDRWTILLDANEPE-------------------CAQERTSMPRS---------------------------THTRVYDKDV-CTCMNNYFGIGVDADLSLGFHNAREEDPGKFNSRLHNKSVYVKVGLQKI--THNRNLHRDIKVQVDNVDIDL---PSVEGLIFLNIPSWGSGADLWG--------------------SDS----------EGKFSKPC-----MHDGLLEVVGVTGVMHMGQVQGGLR-SGIRIAQGQFIRVVLQRETPIQVDGEPWLQPPGNIIV 334          
BLAST of Diacylglycerol kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007068.1 (pep scaffold:Pmarinus_7.0:GL477921:19741:38299:1 gene:ENSPMAG00000006363.1 transcript:ENSPMAT00000007068.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 229.565 bits (584), Expect = 2.310e-66
Identity = 153/415 (36.87%), Postives = 204/415 (49.16%), Query Frame = 1
Query: 1867 PLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAID--KYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDS-GRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISIT 3102
            PLLV INP+SGG QG  ++  F+  +NP QV+DL    GP  GL  FR+V  FR+L+CGGDGTVGW+L  ++  K +    +P VAILPLGTGNDLAR LRWG GY GE    +L  +     V +DRW I      P                    C++ R      +S    G + +SQ    +                       +NNYF IGVDA ++  FH  RE+ P KFNSR+ N+  Y++VG  ++     ++LH  I +  D V +DL     +EG+  LNIPS   G +LWG                    SDS G+F                    M D LLEVVG+   MH G V GG R SG R+AQ + + +  QR  P+Q+DGEPW+Q    I ++
Sbjct:  115 PLLVLINPRSGGMQGRELLHSFRKAVNPHQVFDLACG-GPLPGLHVFREVPRFRLLVCGGDGTVGWVLSLLEDIKLKLACTQPPVAILPLGTGNDLARVLRWGPGYSGEDPLSVLINVDEAQEVKMDRWTILLDANEPE-------------------CAQER------TSMPRLGDETESQYQIVQ-----------------------MNNYFGIGVDADLSLGFHNAREEDPGKFNSRLHNKSVYVKVGLQKI--THNRNLHRDIKVQVDNVDIDL---PSVEGLIFLNIPSWGSGADLWG--------------------SDSEGKFSK----------------PCMHDGLLEVVGVTGVMHMGQVQGGLR-SGIRIAQGQFIRVVLQRETPIQVDGEPWLQPPGNIIVS 438          
BLAST of Diacylglycerol kinase vs. Ensembl Nematostella
Match: EDO42510 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1H8])

HSP 1 Score: 671.389 bits (1731), Expect = 0.000e+0
Identity = 351/758 (46.31%), Postives = 465/758 (61.35%), Query Frame = 1
Query:  892 IDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGR-QGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQL-LEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVL---ERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKK 3147
            + ++++ CYLS+LE GK EDKLEF+FR+YD DGN  LD  E+ECI  QMM+VA YL WD  EL PIL+ M+ E+D D DG ++LEEW +GG+T IPL VLLGL+T +RDDG+H W+ KHF++  +C++CH  L  F R QGL C+FC YTVHERCVQ APASCI+TY K+K++ ++ ++HHW+E + P KC KC K+ K     +G  C WC+  +H  C  +    C+LG ++EHILPP+ I P+ L   +RK+T                   +S+A+  D                  ++    ++   G+        QI  LP T PL VFINPKSGG+QG+R+M KFQY+LNPRQV++L  D GP  GL+FF+ + DFR+L CGGDGTVGW+L  IDK +    RP VA+LPLGTGNDLARCL+WGGGYEG  + K+L+++  G+V+ +DRWQI  ++                                               +D  E GD                P NIINNYFSIGVDAS+A KFH+ REK+PEKFNSR++N+F Y E GTSE L+ATCK+L + I +ICDG +L+L     LEGIAI+NIPSVYGG NLWG                   ++D  + +       S   +LA AVQD+GD  LEVVGLE++++ G +  G R+ G RLAQC  + I+T+R FPMQIDGEPW+Q A T+ I +KNQVPM+  PP  K
Sbjct:   74 VSIREIACYLSMLEGGKVEDKLEFVFRVYDADGNEQLDSQELECITQQMMSVAQYLGWDVTELKPILRDMLVELDDDCDGTISLEEWIKGGLTTIPLRVLLGLETQIRDDGQHQWRQKHFNRPAYCNMCHNQLTGFARKQGLQCLFCKYTVHERCVQRAPASCINTYVKSKRTGKMTMDHHWVEGNCPGKCFKCNKTVKSTNCLTGLRCAWCQMVVHNKCATNVSPECNLGVHREHILPPMSITPAGLVSTDRKRT-------------------NSSAESPD-----------------STTKPNSYVNFDGM------PMQITPLPGTHPLAVFINPKSGGRQGSRLMHKFQYLLNPRQVFNL-GDGGPAPGLKFFQHLSDFRVLCCGGDGTVGWVLATIDKLQM-RFRPPVAVLPLGTGNDLARCLKWGGGYEGGSISKVLSQVQRGSVLSMDRWQIDVTD-----------------------------------------------VDSSENGD--------------SPPLNIINNYFSIGVDASVALKFHLQREKNPEKFNSRLKNKFRYFECGTSETLSATCKNLQDAIQVICDGKILELPN---LEGIAIVNIPSVYGGANLWG-------------------ETDKKKAKKSRSKSGSKDNDLAWAVQDIGDGQLEVVGLESSLYVGQIIAGVRTHGLRLAQCSSIEIKTKRLFPMQIDGEPWMQPAATMKIVHKNQVPMLQGPPPAK 704          
BLAST of Diacylglycerol kinase vs. Ensembl Nematostella
Match: EDO38287 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDC5])

HSP 1 Score: 176.022 bits (445), Expect = 6.595e-45
Identity = 112/324 (34.57%), Postives = 161/324 (49.69%), Query Frame = 1
Query: 1318 CVFCLYTVHERCVQNAPAS---CIHTYTKTKKS---DQLLEHHWIENH-YPAKCIKCKKSTK--LGAKSGFHCRWCKRTIHKCC-MESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNS--QCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            C  C   +H +C++        C  T+ + +     +  + HHW+       +C  C KS +  L  +S +H +       +C  ++   + CDLG +   I+PP  +                + I K + S                            P   NS  +  I+ +     +    +     + +PL+VFINPKSGG QG RIM KFQ++LNPRQV+DL ++ GP  GL+ +R V + R+L CGGDGTVGW+L  +DK ++N  RP VAILPLGTGNDL+R L WGGGY  E L KIL  +  G+VV LDRW +
Sbjct:  146 CTACKIVIHTKCMEKLERINFICKTTFREIQPKGVHESNMRHHWVHRRRQDGRCETCGKSFQHMLSFQSKYHNK------DECFKVDRIEELCDLGVHSNVIVPPSWV----------------VKIPKKARS---------------------------FPTKRNSFKKKSIKRRSTKERKPFVIKPSGSANKRPLVVFINPKSGGNQGLRIMHKFQWLLNPRQVFDLSRE-GPREGLELYRKVPNVRLLACGGDGTVGWILSELDKLKFNP-RPPVAILPLGTGNDLSRALNWGGGYADEPLSKILTHVDEGSVVQLDRWDL 418          

HSP 2 Score: 113.62 bits (283), Expect = 1.691e-25
Identity = 73/239 (30.54%), Postives = 109/239 (45.61%), Query Frame = 1
Query: 2449 SEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEG----IAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFG 3153
            ++  I    +P N++NNYFS+G DA +  +FH  RE HP KFNSR++N+ FY +  ++  +    K  +++  + CDGV +  +    LE     +  LNI     G + WG   +P                           R+  F       Q   D  +EV+ L +A  A    GG    G+RLAQCR V++ T +  PMQ+DGEP       I I+ +NQ  MI     +  G
Sbjct:  426 TDGEIAESRLPLNVMNNYFSLGFDAEVCLEFHESREAHPAKFNSRVKNKLFYGKASSTTFIQGKAKDFYKHTKLECDGVDITEK---LLEAKPMCLLFLNISKYSAGTSPWG---NP--------------------------GRDHEF-----LPQRSDDGYIEVLALTSATLATTRVGGH---GERLAQCRNVIMTTSKSIPMQVDGEPCRLQPSRIRISVRNQADMIQKVKRRALG 624          
BLAST of Diacylglycerol kinase vs. Ensembl Nematostella
Match: EDO34591 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNX6])

HSP 1 Score: 138.658 bits (348), Expect = 2.705e-33
Identity = 70/136 (51.47%), Postives = 91/136 (66.91%), Query Frame = 1
Query: 1855 PDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYE--WNNNRPSVAILPLGTGNDLARCLRWGGGY-EGEKLDKILAKIILGTVVMLDRWQI 2253
            P++ PLLVF+N KSGG QG  I++ FQ +LNP QVY+L+ D GP  GL  F+D+  FRILICGGDGTVGW+L  +D         +P  AI+PLGTGND++R L+WG GY  G+    +L  +    VV LDRW +
Sbjct:  535 PESDPLLVFVNCKSGGGQGDDILSAFQRLLNPHQVYNLM-DGGPLPGLYAFKDLPRFRILICGGDGTVGWVLSCLDDVSTALTYKKPPSAIVPLGTGNDMSRVLQWGSGYSSGDTPLSLLIAVDHAEVVHLDRWFV 669          

HSP 2 Score: 110.923 bits (276), Expect = 1.029e-24
Identity = 73/204 (35.78%), Postives = 100/204 (49.02%), Query Frame = 1
Query: 2488 IINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISI 3099
            ++NNY  IG+DA +   FH+ RE+ PEKF SR+RN+  Y  VG  ++   T     E ++I  DG  L L     LEGI ILNI S   G +LWGP                         +  +  R S++           D L+EVVGL   M  G +  G R SG RLAQ  ++ I+ +   P+QIDGEPW+Q+   + I
Sbjct:  701 VMNNYLGIGIDADLCLDFHLRREEAPEKFTSRLRNKGVYFRVGLRKMANKTKWVFSEEVEIEVDGEKLQL---PTLEGIVILNIGSWAAGADLWGP-------------------------DKDDEFRPSSY----------CDCLVEVVGLTGVMQMGQIQSGIR-SGVRLAQGAQINIKLKCEIPVQIDGEPWMQSPGNVII 865          

HSP 3 Score: 58.151 bits (139), Expect = 1.961e-8
Identity = 42/143 (29.37%), Postives = 58/143 (40.56%), Query Frame = 1
Query: 1204 TNVRDDGKHVWKLKHFS-KSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTK---KSDQLLEHHWIENHYPA--KCIKCKKSTKLG-AKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICI 1611
            T V+    H W    FS +   CS+C + L         C  C Y VHE C   +   C  + T      K     +HHW E ++ A  KC+ CK S   G   +   C WC ++ H  C +   + C  G      LPP C+
Sbjct:   90 TLVKIPVAHTWSGLSFSFRKKFCSVCRRRLEDI--PAFRCQVCDYYVHEACQDFSVTDCKQSATYVPFLGKRAVKQDHHWREGNFSASSKCVSCKHSCGSGECLASLKCSWCAQSSHTNCSQLLPKECHYGYLHRVSLPPFCV 230          
BLAST of Diacylglycerol kinase vs. Ensembl Nematostella
Match: EDO48435 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJW1])

HSP 1 Score: 123.25 bits (308), Expect = 2.966e-29
Identity = 99/301 (32.89%), Postives = 141/301 (46.84%), Query Frame = 1
Query: 1423 HHWIENHY---PAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQ--TCDL------GDNKEHILP---PICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDC---QXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTK---PLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFR--ILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEK-LDKILAKIILGTVVMLDRWQIT 2256
            HHW    +   P+ C  C+ +       G HC  C  ++H  C+E+  +  TC           K H +    P+C Y SV           C    +  +   +        DC   Q   C        ILP    S   +  +   + Q    +    P+T+   PLLVF N KSG   G R++  F+ +LNP QV DL  +  P   L+F R +   R  +L+CGGDG+VGW+L+A+DK +   + P + ILPLGTGNDLAR L WG GY GE+  + +L  I+   V  LDRW++T
Sbjct:   49 HHWFFTEFLDKPSYCNACENALV----RGLHCENCTLSVHDECLETANEVFTCKALVLSRRSSLKHHYIRGNLPLCSYCSVC-------GCLCGTAPRLCDQRCIWCQEKVHDDCLRNQSMICDFGRYRTLILPPHCISLTLVGWR--KNKQRFVVKEITPPNTRNWSPLLVFANCKSGDNDGERLLQAFRGVLNPVQVIDL-HEVPPETALEFCRLLPGHRCRVLVCGGDGSVGWVLDALDKVKLKLS-PYIGILPLGTGNDLARVLGWGSGYAGEEDANDVLNSILKADVTELDRWKVT 334          

HSP 2 Score: 106.301 bits (264), Expect = 1.095e-23
Identity = 70/213 (32.86%), Postives = 99/213 (46.48%), Query Frame = 1
Query: 2491 INNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREV-VIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMI 3126
            +NNYFS+G DA +   FH  RE  P  F SR+ N+  Y   G  +VL   CK+LHE +++  D   ++L     LEGI ILNI S  GG ++W                      D GR  +                    D LLEVVGL +++H   +     +   R+ Q  ++      +  P+Q+DGEPW QA C I+IT+ NQ  M+
Sbjct:  352 MNNYFSVGCDAKVVLNFHRHRESQPTLFTSRLFNKAMYGVYGARDVLQQECKNLHEMVELELDDKKVELPD---LEGIVILNISSWCGGCDMWNSC-----------------SDDDGRPPT------------------ASDGLLEVVGLYSSLHIARLQVSL-ADPHRIGQAHKIKASENAKHLPVQVDGEPWEQAPCVITITHHNQALML 525          

HSP 3 Score: 56.6102 bits (135), Expect = 4.390e-8
Identity = 40/139 (28.78%), Postives = 60/139 (43.17%), Query Frame = 1
Query: 1222 GKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPA--SCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGF-------HCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICI 1611
            G H +  +   K ++C+ C   LVR    GL C  C  +VH+ C++ A    +C       + S   L+HH+I  + P     C   +  G   G         C WC+  +H  C+ +    CD G  +  ILPP CI
Sbjct:   48 GHHWFFTEFLDKPSYCNACENALVR----GLHCENCTLSVHDECLETANEVFTCKALVLSRRSS---LKHHYIRGNLPL----CSYCSVCGCLCGTAPRLCDQRCIWCQEKVHDDCLRNQSMICDFGRYRTLILPPHCI 175          
BLAST of Diacylglycerol kinase vs. Ensembl Nematostella
Match: EDO40684 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6N0])

HSP 1 Score: 117.472 bits (293), Expect = 5.990e-27
Identity = 65/141 (46.10%), Postives = 89/141 (63.12%), Query Frame = 1
Query: 1855 PDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGE-KLDKILAKIILGTVVMLDRWQITCSEPAP 2274
            P   PLLVF+N KSG  QG + + KF+ +LNP QV+DL    GP NGL+FFR  + FRIL+CGGDG+VGW+++ +D  +    +  + +LPLGTGNDL+R L WG  +  E  + + L  +     +MLDRW I   E  P
Sbjct:  235 PRNSPLLVFVNSKSGDNQGVKFIRKFKQLLNPLQVFDL-GVGGPTNGLKFFRRFRRFRILVCGGDGSVGWVMKEVDLMKM-ATQCQIGVLPLGTGNDLSRVLGWGTSFADENSVPQFLQHLERAKPLMLDRWAIMVQECNP 373          

HSP 2 Score: 86.6557 bits (213), Expect = 2.634e-17
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 1
Query: 2488 IINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWG 2772
            ++NNYF IG+DA I   FH  RE+HPEK+ SR RN  +Y  +G  E++  T K++ + +   CDG  + L     L+GI +LNI S  GG N WG
Sbjct:  602 VMNNYFGIGIDAKITLDFHTRREEHPEKYRSRTRNMIWYGVLGGKEIVQRTYKNIDQRVK--CDGHRISL---PSLQGIVVLNITSYMGGANFWG 691          

HSP 3 Score: 76.6406 bits (187), Expect = 3.625e-14
Identity = 46/144 (31.94%), Postives = 66/144 (45.83%), Query Frame = 1
Query: 1222 GKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSD---------QLLEHHWIE-NHYP-AKCIKCKKSTKLGAKS---GFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICI 1611
            G H W     ++ T C++C  IL     +GL C  C +  H+RC   AP +C  T  +  + D           + H W+E N  P A+C  C++    G+K     F C WC  T+H  C  S    C LG+N   +LPP+ I
Sbjct:   80 GLHNWYSCAHARPTFCNVCKDILSGVTSKGLSCEVCRFKSHKRCAAKAPNNCKWTTMQAIRKDNEDCDNSDPMSMPHQWLEGNLTPGARCTVCERPC--GSKRRLLDFRCLWCNMTVHHSCKLSVVSKCTLGENSLSVLPPMFI 221          
BLAST of Diacylglycerol kinase vs. Ensembl Medaka
Match: dgkb (diacylglycerol kinase beta [Source:NCBI gene;Acc:101159850])

HSP 1 Score: 509.99 bits (1312), Expect = 8.751e-166
Identity = 268/493 (54.36%), Postives = 325/493 (65.92%), Query Frame = 1
Query:  823 SALTLSKSNNLDI----VNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRR 2286
            S L L K+N+  +    + RP+   D + +KD++ YLSLLE G+PEDKLEFMFRLYDTDGNG LD SE+E IISQMM VA YL WD  EL PILQ+MM EIDYD DG V+LEEW +GG+T IPLLVLLGL+TNV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+KK+ +++ H W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VLER+ T + KD  + +     M  A+S                               +  QGL        QI  +  T PLLVF+NPKSGGKQG RI  KFQY+LNPRQVY+L K+ GP  GL FFRD+ D R+L CGGDGTVGW+L+ IDK   + N P V ILPLGTGNDLARCLRWGGGYEGE L KIL  I   T VMLDRW+I  + PA +  R
Sbjct:  105 SGLKLIKANSTPLKTPTLPRPV---DSVQLKDIVFYLSLLEGGRPEDKLEFMFRLYDTDGNGSLDSSELEHIISQMMRVAEYLEWDVTELKPILQEMMQEIDYDQDGTVSLEEWIQGGLTTIPLLVLLGLETNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGLGKQGLCCSFCKYTVHERCVGRAPHSCIKTYVKSKKNTEIMHHFWVEGNCPTKCDKCHKTIKCYQGLTGLHCVWCQITLHNKCASHVKPECDCGPLKDHILPPNSICPIVLERQSTLR-KDSRSSQSGRGKMQRANSVT-----------------------------VDGQGL--------QITTIEGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYNLAKN-GPMPGLNFFRDLPDCRVLACGGDGTVGWILDFIDKANMDKN-PPVCILPLGTGNDLARCLRWGGGYEGESLFKILRDIENSTQVMLDRWKIDVT-PADKEER 553          

HSP 2 Score: 249.21 bits (635), Expect = 2.492e-69
Identity = 122/235 (51.91%), Postives = 158/235 (67.23%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR 3174
            D +P +IINNYFSIGVDASIAH+FHV+REKHPEKFNSR +N+ +Y E GTSE  +ATCK LH+++++ CDGV+LDL     LEGIAILNIPS++GG NLWG             +K ++     G+    + +     + L  AVQD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  V IRT +  PMQIDGEPW+Q  CTI I +KNQ PM++ PP  +    ++  R
Sbjct:  555 DPVPYSIINNYFSIGVDASIAHRFHVMREKHPEKFNSRTKNKLWYFEFGTSETFSATCKKLHDFLEVECDGVILDL-SSISLEGIAILNIPSMHGGSNLWG------------ESKKRRGHRKGGKKSQEKRTPVLDPKELMFAVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTSKSLPMQIDGEPWMQTPCTIEIVHKNQAPMLIGPPQSRSFFSSVLRR 776          
BLAST of Diacylglycerol kinase vs. Ensembl Medaka
Match: dgkg (diacylglycerol kinase beta [Source:NCBI gene;Acc:105356367])

HSP 1 Score: 436.417 bits (1121), Expect = 4.735e-139
Identity = 233/463 (50.32%), Postives = 297/463 (64.15%), Query Frame = 1
Query:  871 PLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSF-QICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            P T    + +KD++CYLSLLE G+PEDKLEFMFRLYDTDGNG+LD SE++ II+QM++VA YL+WD+ EL PIL++MM EIDYD+DG VTLEEW RGG+T IPLLVLLG++T V +DG+HVW+LKHF+K  +C+ CH +L+   +QGLCC  C YTVHERCV    A+CI TY K+++    + H W+E + P+ C +C +S K     +G HC WC+ T    C    +  CD G  ++H L P  I P VL R        C                                     PQ S S   +Q Q  +  + S+  +I  LP T PLLV +NPKSGG+QG R++ KF+Y+LNPRQVY L +  GP  GL FF DV DFR+L CGGDGTVGW+L+ I+K  ++ + P VAILPLGTGNDLARCLRWGGGYEG  L K+L  I     V+LDRW I
Sbjct:   46 PHTPPQVVLLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGLLDSSELDRIINQMVHVAEYLDWDSTELRPILKEMMEEIDYDHDGTVTLEEWIRGGLTTIPLLVLLGMET-VCEDGQHVWRLKHFNKPAYCNFCHTVLLGVRKQGLCCSLCKYTVHERCVSKDIAACISTYAKSRRHTNAMLHVWMEGNCPSNCDRCHRSIKCYQGLTGLHCVWCQIT----CASHVKPECDGGVLRDHTLLPSYICPVVLVRCPLC----CF----------------------------------CCPQLSGS--ILQLQPKHGGRWSTPEEITPLPGTCPLLVLVNPKSGGRQGERVLRKFRYLLNPRQVYSL-EQGGPMVGLNFFHDVPDFRVLACGGDGTVGWILDCIEKANFSKH-PPVAILPLGTGNDLARCLRWGGGYEGGSLLKVLRDIEHSEEVVLDRWHI 461          

HSP 2 Score: 218.779 bits (556), Expect = 1.144e-59
Identity = 109/213 (51.17%), Postives = 142/213 (66.67%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYK 3108
            D +P +I+NNYFSIGVDASIAH+FH++REKHPEKFNSRM+N+ +Y E GT+E ++ATCK L+E I++ CDGV LDL   + LEGIA+LNIPS++GG NLWG                        R     +S++ T +  +  V D  D LLEVVGLE A+  G +  G +S+G+RLAQC  V IRT R  PMQIDGEPW+Q  CT++ + K
Sbjct:  473 DPVPNSIVNNYFSIGVDASIAHRFHLMREKHPEKFNSRMKNKLWYFEFGTTETISATCKKLNECIEVECDGVTLDLSNTS-LEGIAVLNIPSMHGGSNLWG--------------------ETKKRRNYSRMSKKVTDKIPSITVTDFSDQLLEVVGLEGAIEMGQIYTGLKSAGRRLAQCASVTIRTSRRLPMQIDGEPWMQPPCTVNASIK 664          
BLAST of Diacylglycerol kinase vs. Ensembl Medaka
Match: dgkb (diacylglycerol kinase beta [Source:NCBI gene;Acc:101159850])

HSP 1 Score: 430.254 bits (1105), Expect = 1.929e-135
Identity = 242/499 (48.50%), Postives = 302/499 (60.52%), Query Frame = 1
Query:  823 SALTLSKSNNLDI----VNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTK-LGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLER-----KQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICA-LPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRR 2286
            S L L K+N+  +    + RP+   D + +KD++ YLSLLE G+PEDKLE    L++     +L + E+E IISQMM VA YL WD  EL PILQ+MM EIDYD DG V+LEEW +GG+T IPLLVLLGL+TNV+DDG+HVW+LKHF+K  +C+LC  +L+  G+QGLCC FC YTVHERCV  AP SCI TY K+KK+ +++ H W+E + P KC KC K+ K     +G HC WC+ T+H  C    +  CD G  K+HILPP  I P VL R     +Q+   KD  + +     M  A+S    VD Q                    Q    + G       SF   +  P  K                 I  KFQY+LNPRQVY+L K+ GP  GL FFRD+ D R+L CGGDGTVGW+L+ IDK   + N P V ILPLGTGNDLARCLRWGGGYEGE L KIL  I   T VMLDRW+I  + PA +  R
Sbjct:  108 SGLKLIKANSTPLKTPTLPRPV---DSVQLKDIVFYLSLLEGGRPEDKLE----LFEAFHGCLLWRQELEHIISQMMRVAEYLEWDVTELKPILQEMMQEIDYDQDGTVSLEEWIQGGLTTIPLLVLLGLETNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGLGKQGLCCSFCKYTVHERCVGRAPHSCIKTYVKSKKNTEIMHHFWVEGNCPTKCDKCHKTIKCYQGLTGLHCVWCQITLHNKCASHVKPECDCGPLKDHILPPNSICPIVLVRTPSPERQSTLRKDSRSSQSGRGKMQRANSVT--VDGQGL------------------QVRTPDVGQTQMHFHSFTTRSHFPQMK-----------------IYRKFQYLLNPRQVYNLAKN-GPMPGLNFFRDLPDCRVLACGGDGTVGWILDFIDKANMDKN-PPVCILPLGTGNDLARCLRWGGGYEGESLFKILRDIENSTQVMLDRWKIDVT-PADKEER 559          

HSP 2 Score: 248.825 bits (634), Expect = 3.291e-69
Identity = 122/235 (51.91%), Postives = 158/235 (67.23%), Query Frame = 1
Query: 2470 DEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR 3174
            D +P +IINNYFSIGVDASIAH+FHV+REKHPEKFNSR +N+ +Y E GTSE  +ATCK LH+++++ CDGV+LDL     LEGIAILNIPS++GG NLWG             +K ++     G+    + +     + L  AVQD+ D LLEVVGLE AM  G +  G +S+G+RLAQC  V IRT +  PMQIDGEPW+Q  CTI I +KNQ PM++ PP  +    ++  R
Sbjct:  561 DPVPYSIINNYFSIGVDASIAHRFHVMREKHPEKFNSRTKNKLWYFEFGTSETFSATCKKLHDFLEVECDGVILDL-SSISLEGIAILNIPSMHGGSNLWG------------ESKKRRGHRKGGKKSQEKRTPVLDPKELMFAVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTSKSLPMQIDGEPWMQTPCTIEIVHKNQAPMLIGPPQSRSFFSSVLRR 782          
BLAST of Diacylglycerol kinase vs. Ensembl Medaka
Match: dgkaa (diacylglycerol kinase alpha [Source:NCBI gene;Acc:101154951])

HSP 1 Score: 421.009 bits (1081), Expect = 1.253e-132
Identity = 222/456 (48.68%), Postives = 282/456 (61.84%), Query Frame = 1
Query:  889 KIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIE-NHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            ++ +KD+ CY SLLE G+P DKLEF FRLYD DGNGVLD SEV+ II+QMM+ A YL+WD  EL P+L+ MM  ID D+ G V+LEEW  GGM N+PLLVLLGL    + DG+H+W++K F+K  +C+ C   L    +QGLCC  C YTVH RC    PA C  TY K+KK   +  H W+  N    KC +C+K  K+ +  G HC WC    H  C E     C  G  K+HILPP  IYP V ER    KN               +  +AD                       +S+  I   G         QI  +P+T PLLVF+NPKSGGKQG R++ KFQY+LNPRQVY+L  + GP  GL FF+D++++RIL+CGGDGTVGW+L+AIDK +    RP VA+LPLGTGNDLARCLRWGGGY+GE L +IL +I   ++V +DRW +
Sbjct:   96 EVFLKDVSCYFSLLEDGQPRDKLEFAFRLYDRDGNGVLDSSEVDRIIAQMMHAAEYLDWDVSELKPVLKDMMTAIDADSSGTVSLEEWVEGGMNNVPLLVLLGLKM-TQKDGQHLWRMKRFNKRVNCNKCRSPL-GLRKQGLCCTCCKYTVHGRCANCDPAPCTRTYVKSKKEAGVAAHDWVSGNCDSKKCDRCQK--KIKSFQGKHCVWCHTMRHDECAELEPTECTCGLLKDHILPPWAIYPVVKERPNNMKNG--------------SSGSAD-----------------------DSELNITPDG------QVLQILPVPNTHPLLVFVNPKSGGKQGERVLNKFQYLLNPRQVYNL-SNGGPGPGLSFFKDLQEYRILVCGGDGTVGWILDAIDKAKL-LVRPPVAVLPLGTGNDLARCLRWGGGYDGEDLSRILKEIEGSSLVPMDRWSV 502          

HSP 2 Score: 211.075 bits (536), Expect = 1.051e-56
Identity = 114/241 (47.30%), Postives = 150/241 (62.24%), Query Frame = 1
Query: 2443 VLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFEN-LATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRN 3162
            +  E+    D +P  IINNYFSIGVDASIAH+FH +REKHP+KFNSRM+N+ +Y E  TSE ++A+CK L+E + I C G  LDL     LEGIA+LNIPS++GG NLWG                + KK D+    S+E        + L    QD+ D  LEVVGL+ A   G +  G +S+ +RLA+  ++ I+T++  PMQIDGEPW+Q  CTI IT+KNQ  M+MAP  K  G  N
Sbjct:  505 ITDENQEKGDPVPYEIINNYFSIGVDASIAHRFHTMREKHPQKFNSRMKNKLWYFEFATSETISASCKKLNESLTIECCGTPLDL-SSVSLEGIAVLNIPSMHGGSNLWG----------------ETKKGDAKGLTSQEEPEVIIDPDILKVTSQDLSDRRLEVVGLKGATEMGQIYTGLKSA-ERLAKTSQITIKTKKALPMQIDGEPWMQPPCTIHITHKNQACMLMAPQSKSSGFFN 727          
BLAST of Diacylglycerol kinase vs. Ensembl Medaka
Match: dgkab (diacylglycerol kinase beta [Source:NCBI gene;Acc:101165301])

HSP 1 Score: 375.555 bits (963), Expect = 1.474e-115
Identity = 199/463 (42.98%), Postives = 268/463 (57.88%), Query Frame = 1
Query:  874 LTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIE-NHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDC--LNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQI 2253
            +  + ++ ++D+ CY S+LE  +P +KLEF F+LYD DGNG+LD SEV+ II+QMM  A YL WD  EL P+L+ MM  ID D+ G VTLEEW +GGM N+PLLVLLGL   +  DG+H+W++KHF+K T+CS C  +L+  G+QGLCC+ C YTVH +C      +C  T+ K+K+      H WI+ +   +KC  C K  K+   +G  C WC+   H+ C+      C  G  K+HILPP  IY         +K +D   LN+      + +A                       +P +     F+                            NPKSGGKQG R++ KFQ +LNPRQVY+L  + GP  GL FFR +  +RIL+CGGDGTVGWLL+AID+     + P VA+LPLGTGNDLARCLRWGGGYEG  L +IL +I    V+ +DRW +
Sbjct:   95 MPKEGRVYLRDVSCYFSVLEEAQPREKLEFTFKLYDADGNGLLDSSEVDRIITQMMRAADYLGWDVTELRPVLKDMMTAIDVDSSGTVTLEEWVKGGMNNVPLLVLLGLKM-IEKDGQHMWRMKHFNKPTYCSQCENMLLGLGKQGLCCICCSYTVHNQCANKKLETCPPTFVKSKQEIGKSSHGWIKVDCNNSKCEVCSK--KIKNLTGKRCVWCQEMRHEACVLPGIPRCGCGPLKDHILPPWAIY-------AVSKEEDSCLLNVTPDGHILQIAQ----------------------IPDTHPLLVFV----------------------------NPKSGGKQGERVLRKFQGLLNPRQVYNL-SNGGPTPGLHFFRTLTQYRILVCGGDGTVGWLLDAIDRANLQVH-PPVAVLPLGTGNDLARCLRWGGGYEGTDLREILKEIEKSEVIPVDRWSV 495          

HSP 2 Score: 229.18 bits (583), Expect = 7.490e-63
Identity = 120/233 (51.50%), Postives = 151/233 (64.81%), Query Frame = 1
Query: 2467 SDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNM 3165
             D +P  IINNYFSIGVDASIAH+FH +REKHP++FNSRM+N+ +Y E  TSE ++A+CK L+E + I C G  LDL + A LEGIAILNIPS++GG NLWG    P  +            S+ GR E       +  E+L T  QDM D   EVVGLE AM  G +  G +S+G RLAQ  ++ IRT +  PMQIDGEPW+Q  CTI IT+KNQ  M+MA P K  G  ++
Sbjct:  506 GDPVPQEIINNYFSIGVDASIAHRFHSMREKHPQRFNSRMKNKLWYFEFATSETISASCKKLNESLVIECCGKRLDLSRVA-LEGIAILNIPSMHGGSNLWGESKKPDSV------------SEVGRGEVI-----TDPEHLKTVTQDMSDKRFEVVGLEGAMEMGQIYTGLKSAGHRLAQASQITIRTTKALPMQIDGEPWMQPPCTIHITHKNQANMLMAQPTKPSGFFHL 720          
BLAST of Diacylglycerol kinase vs. Planmine SMEST
Match: SMESG000075921.1 (SMESG000075921.1)

HSP 1 Score: 1998.4 bits (5176), Expect = 0.000e+0
Identity = 1015/1031 (98.45%), Postives = 1017/1031 (98.64%), Query Frame = 1
Query:   82 MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSKINEDNGKRIIYESRIRNNSLNATSTNAILSNLSERLSAIAHXXXXXXXXXXXXXYVCHNNLQCCQSSISHQRCRTLPLNHIDSTFNVFGKKYKSNLGSAHSHPSLVTHTIQPIFPNEDTPIHHXXXXXXXXXXXIENEIVCEKSALTLSKSNNLDIVNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR 3174
            MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSKINEDNGKRI+YESRIRNNSLNATSTNAILSNLSERLSAIAHSSTILSSKSIFSTYVCHNNLQCCQSSISHQRCRTLPLNHIDSTFNVFGKKYKSNLGSAHSHPSLVTHTIQPIFPNEDTPIHHSRSSSKKSDKKIENEIVCEKSALTLSKSNNLDIVNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQENNCGNNSNSENILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRK              VYGGKNLWGPVISPSKIKKNQ+NKPKKKKSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR
Sbjct:    1 MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSKINEDNGKRIVYESRIRNNSLNATSTNAILSNLSERLSAIAHSSTILSSKSIFSTYVCHNNLQCCQSSISHQRCRTLPLNHIDSTFNVFGKKYKSNLGSAHSHPSLVTHTIQPIFPNEDTPIHHSRSSSKKSDKKIENEIVCEKSALTLSKSNNLDIVNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQENNCGNNSNSENILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRK--------------VYGGKNLWGPVISPSKIKKNQINKPKKKKSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR 1017          
BLAST of Diacylglycerol kinase vs. Planmine SMEST
Match: SMESG000075921.1 (SMESG000075921.1)

HSP 1 Score: 1991.08 bits (5157), Expect = 0.000e+0
Identity = 1014/1031 (98.35%), Postives = 1016/1031 (98.55%), Query Frame = 1
Query:   82 MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSKINEDNGKRIIYESRIRNNSLNATSTNAILSNLSERLSAIAHXXXXXXXXXXXXXYVCHNNLQCCQSSISHQRCRTLPLNHIDSTFNVFGKKYKSNLGSAHSHPSLVTHTIQPIFPNEDTPIHHXXXXXXXXXXXIENEIVCEKSALTLSKSNNLDIVNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR 3174
            MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSKINEDNGKRI+YESRIRNNSLNATSTNAILSNLSERLSAIAHSSTILSSKSIFSTYVCHNNLQCCQSSISHQRCRTLPLNHIDSTFNVFGKKYKSNLGSAHSHPSLVTHTIQPIFPNEDTPIHHSRSSSKKSDKKIENEIVCEKSALTLSKSNNLDIVNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQENNCGNNSNSENILPQSSNSQCFIQE GLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRK              VYGGKNLWGPVISPSKIKKNQ+NKPKKKKSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR
Sbjct:    1 MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEKPIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPTLKSKINEDNGKRIVYESRIRNNSLNATSTNAILSNLSERLSAIAHSSTILSSKSIFSTYVCHNNLQCCQSSISHQRCRTLPLNHIDSTFNVFGKKYKSNLGSAHSHPSLVTHTIQPIFPNEDTPIHHSRSSSKKSDKKIENEIVCEKSALTLSKSNNLDIVNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYDTDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDGEVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHWIENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDNKEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQENNCGNNSNSENILPQSSNSQCFIQE-GLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLRK--------------VYGGKNLWGPVISPSKIKKNQINKPKKKKSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAPPLKKFGLRNMFSR 1016          
BLAST of Diacylglycerol kinase vs. Planmine SMEST
Match: SMESG000051710.1 (SMESG000051710.1)

HSP 1 Score: 282.337 bits (721), Expect = 3.316e-82
Identity = 207/644 (32.14%), Postives = 284/644 (44.10%), Query Frame = 1
Query: 1318 CVFCLYTVHERCVQNAPASC--------IHTYTKTKKSDQLLEHHWI-ENHYPAKCIKCKKS--TKLGAKS----GFHCRWCKRTIHK---CCMESW-RQTCDLGDNKEHILPPICIY-----------------PSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDS-SSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            C  C   +H+ C+ +    C        +  Y   KK +  L HHW+       KC +C KS  +K    S       C WCK   H    C   S   + C  G + + I+PP  I                  P  + R  +A  +D   I K +   N++  +   +D        + +   I   S  S   I     NH       I   P +KPLLVF+NPKSGG  G ++M KFQ++LNPRQV+D+  D+GP  GL+ F  + + RIL+CGGDGTVGW++  ID      + P +AILPLGTGNDLAR   WG GY  E L KI++ +  G +  LDRW +            + T N C RT           +++D  S QN  G  E S      +                  P N+ NNYFS+G DA+ A +FH  RE +PEKFNSR++N+ FY  +G  +VL  + K L +YI ++CDG+    R K      +  LNI    GG   WG                    + +G F                  Q   D  LEVVGL  A  A L  GG      R+ QC+  V+ T +  PMQ+DGEP       ISI++     +I+ P
Sbjct:   85 CSSCKLIIHQLCIPHLQLPCRPTFREAGVRDYRDEKKKEDTLRHHWVHRKRQDGKCKQCGKSFQSKFTINSKEIVAVQCSWCKSPYHNKTLCFATSLLTEECSRGQHYDSIIPPSWIIKMATKNNFKSSVRKSRSPDTMTR--SALPQDSTFIPKPANPDNISSPSKLHID-----DNISEHITMIKSISLQSTFGIT----NHIPFVIKPIPNSPPSKPLLVFLNPKSGGNAGVKLMRKFQWLLNPRQVFDIT-DRGPRLGLELFGKIANVRILVCGGDGTVGWVMSEIDSLAIFPS-PPIAILPLGTGNDLARATHWGSGYTDESLSKIISCVEEGQIKPLDRWNL------------ASTPNQCPRT-----------ISKDELSMQNKDGTSEQSNPPAVSK-----------------FPLNVFNNYFSLGADAATALEFHESREANPEKFNSRLKNKIFYAGIGGKDVLKRSWKDLCDYITLVCDGIDFTPRIKEMKPHLLLFLNIQRYSGGTQPWG--------------------NPAGGF----------------GPQSFNDGKLEVVGLTAASLATLQVGGH---ADRICQCQNAVVTTIKTIPMQVDGEPCRLLPSEISISHHGIAQIILKP 636          
BLAST of Diacylglycerol kinase vs. Planmine SMEST
Match: SMESG000051710.1 (SMESG000051710.1)

HSP 1 Score: 281.952 bits (720), Expect = 4.488e-82
Identity = 207/644 (32.14%), Postives = 284/644 (44.10%), Query Frame = 1
Query: 1318 CVFCLYTVHERCVQNAPASC--------IHTYTKTKKSDQLLEHHWI-ENHYPAKCIKCKKS--TKLGAKS----GFHCRWCKRTIHK---CCMESW-RQTCDLGDNKEHILPPICIY-----------------PSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDS-SSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            C  C   +H+ C+ +    C        +  Y   KK +  L HHW+       KC +C KS  +K    S       C WCK   H    C   S   + C  G + + I+PP  I                  P  + R  +A  +D   I K +   N++  +   +D        + +   I   S  S   I     NH       I   P +KPLLVF+NPKSGG  G ++M KFQ++LNPRQV+D+  D+GP  GL+ F  + + RIL+CGGDGTVGW++  ID      + P +AILPLGTGNDLAR   WG GY  E L KI++ +  G +  LDRW +            + T N C RT           +++D  S QN  G  E S      +                  P N+ NNYFS+G DA+ A +FH  RE +PEKFNSR++N+ FY  +G  +VL  + K L +YI ++CDG+    R K      +  LNI    GG   WG                    + +G F                  Q   D  LEVVGL  A  A L  GG      R+ QC+  V+ T +  PMQ+DGEP       ISI++     +I+ P
Sbjct:   85 CSSCKLIIHQLCIPHLQLPCRPTFREAGVRDYRDEKKKEDTLRHHWVHRKRQDGKCKQCGKSFQSKFTINSKEIVAVQCSWCKSPYHNKTLCFATSLLTEECSRGQHYDSIIPPSWIIKMATKNNFKSSVRKSRSPDTMTR--SALPQDSTFIPKPANPDNISSPSKLHID-----DNISEHITMIKSISLQSTFGIT----NHIPFVIKPIPNSPPSKPLLVFLNPKSGGNAGVKLMRKFQWLLNPRQVFDIT-DRGPRLGLELFGKIANVRILVCGGDGTVGWVMSEIDSLAIFPS-PPIAILPLGTGNDLARATHWGSGYTDESLSKIISCVEEGQIKPLDRWNL------------ASTPNQCPRT-----------ISKDELSMQNKDGTSEQSNPPAVSK-----------------FPLNVFNNYFSLGADAATALEFHESREANPEKFNSRLKNKIFYAGIGGKDVLKRSWKDLCDYITLVCDGIDFTPRIKEMKPHLLLFLNIQRYSGGTQPWG--------------------NPAGGF----------------GPQSFNDGKLEVVGLTAASLATLQVGGH---ADRICQCQNAVVTTIKTIPMQVDGEPCRLLPSEISISHHGIAQIILKP 636          
BLAST of Diacylglycerol kinase vs. Planmine SMEST
Match: SMESG000051710.1 (SMESG000051710.1)

HSP 1 Score: 283.878 bits (725), Expect = 4.699e-80
Identity = 214/675 (31.70%), Postives = 297/675 (44.00%), Query Frame = 1
Query: 1228 HVWKLKHFSKSTHCSLCHKILVRFGRQGLCCVFCLYTVHERCVQNAPASC--------IHTYTKTKKSDQLLEHHWI-ENHYPAKCIKCKKS--TKLGAKS----GFHCRWCKRTIHK---CCMESW-RQTCDLGDNKEHILPPICIY-----------------PSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQXXXXXXXXXXXXILPQSSNSQCFIQEQGL-NHNQISSFQICALPDTKPLLVFINPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRILICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYEGEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTGYCSRNRGVNQDS-SSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMPCNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVLTATCKHLHEYIDIICDGVVLDLR-KGAYLEGIAILNIPSVYGGKNLWGPVISPSXXXXXXXXXXXXXXSDSGRFESRELSRESTFENLATAVQDMGDNLLEVVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQAACTISITYKNQVPMIMAP 3135
            H+W +   S    C L   +  + G +  C   C   +H+ C+ +    C        +  Y   KK +  L HHW+       KC +C KS  +K    S       C WCK   H    C   S   + C  G + + I+PP  I                  P  + R  +A  +D   I K +   N++  +   +D       N S    ++   S    F    G+ NH       I   P +KPLLVF+NPKSGG  G ++M KFQ++LNPRQV+D+  D+GP  GL+ F  + + RIL+CGGDGTVGW++  ID      + P +AILPLGTGNDLAR   WG GY  E L KI++ +  G +  LDRW +            + T N C RT           +++D  S QN  G  E S      +                  P N+ NNYFS+G DA+ A +FH  RE +PEKFNSR++N+ FY  +G  +VL  + K L +YI ++CDG+    R K      +  LNI    GG   WG                    + +G F                  Q   D  LEVVGL  A  A L  GG      R+ QC+  V+ T +  PMQ+DGEP       ISI++     +I+ P
Sbjct:   57 HLW-VDTNSSGDFCYLNENVCEKGGAKRKCSS-CKLIIHQLCIPHLQLPCRPTFREAGVRDYRDEKKKEDTLRHHWVHRKRQDGKCKQCGKSFQSKFTINSKEIVAVQCSWCKSPYHNKTLCFATSLLTEECSRGQHYDSIIPPSWIIKMATKNNFKSSVRKSRSPDTMTR--SALPQDSTFIPKPANPDNISSPSKLHID------DNISEHITMIKSISLQSTF----GITNHIPFVIKPIPNSPPSKPLLVFLNPKSGGNAGVKLMRKFQWLLNPRQVFDIT-DRGPRLGLELFGKIANVRILVCGGDGTVGWVMSEIDSLAIFPS-PPIAILPLGTGNDLARATHWGSGYTDESLSKIISCVEEGQIKPLDRWNL------------ASTPNQCPRT-----------ISKDELSMQNKDGTSEQSNPPAVSK-----------------FPLNVFNNYFSLGADAATALEFHESREANPEKFNSRLKNKIFYAGIGGKDVLKRSWKDLCDYITLVCDGIDFTPRIKEMKPHLLLFLNIQRYSGGTQPWG--------------------NPAGGF----------------GPQSFNDGKLEVVGLTAASLATLQVGGH---ADRICQCQNAVVTTIKTIPMQVDGEPCRLLPSEISISHHGIAQIILKP 636          
The following BLAST results are available for this feature:
BLAST of Diacylglycerol kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DGKB1.061e-16255.38diacylglycerol kinase beta [Source:HGNC Symbol;Acc... [more]
DGKB2.924e-16255.38diacylglycerol kinase beta [Source:HGNC Symbol;Acc... [more]
DGKB2.924e-16255.38diacylglycerol kinase beta [Source:HGNC Symbol;Acc... [more]
DGKB1.600e-16155.16diacylglycerol kinase beta [Source:HGNC Symbol;Acc... [more]
DGKB1.627e-16155.16diacylglycerol kinase beta [Source:HGNC Symbol;Acc... [more]
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BLAST of Diacylglycerol kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dgk-30.000e+046.44Probable diacylglycerol kinase 3 [Source:UniProtK... [more]
dgk-56.312e-6430.17Putative diacylglycerol kinase K06A1.6 [Source:Un... [more]
dgk-58.040e-6430.17Putative diacylglycerol kinase K06A1.6 [Source:Un... [more]
dgk-59.169e-6430.17Putative diacylglycerol kinase K06A1.6 [Source:Un... [more]
dgk-51.138e-6330.17Putative diacylglycerol kinase K06A1.6 [Source:Un... [more]
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BLAST of Diacylglycerol kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Dgk8.016e-15450.51gene:FBgn0085390 transcript:FBtr0112581[more]
Dgk7.844e-15351.26gene:FBgn0085390 transcript:FBtr0112580[more]
Dgk4.430e-15249.08gene:FBgn0085390 transcript:FBtr0346954[more]
Dgk8.455e-15249.08gene:FBgn0085390 transcript:FBtr0330238[more]
Dgk2.902e-15149.08gene:FBgn0085390 transcript:FBtr0308589[more]
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BLAST of Diacylglycerol kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dgkaa0.000e+044.56diacylglycerol kinase, alpha a [Source:ZFIN;Acc:ZD... [more]
dgkaa0.000e+044.56diacylglycerol kinase, alpha a [Source:ZFIN;Acc:ZD... [more]
dgkab0.000e+040.83diacylglycerol kinase, alpha b [Source:ZFIN;Acc:ZD... [more]
dgkaa0.000e+042.78diacylglycerol kinase, alpha a [Source:ZFIN;Acc:ZD... [more]
dgkg2.196e-15352.75diacylglycerol kinase, gamma [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of Diacylglycerol kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DGKA1.499e-13549.67diacylglycerol kinase alpha [Source:NCBI gene;Acc:... [more]
DGKA1.770e-13549.67diacylglycerol kinase alpha [Source:NCBI gene;Acc:... [more]
DGKA1.295e-11745.30diacylglycerol kinase alpha [Source:NCBI gene;Acc:... [more]
DGKZ2.014e-7531.60diacylglycerol kinase, zeta [Source:NCBI gene;Acc:... [more]
DGKZ2.221e-7531.25diacylglycerol kinase, zeta [Source:NCBI gene;Acc:... [more]
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BLAST of Diacylglycerol kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Dgkg3.992e-845.50diacylglycerol kinase, gamma [Source:MGI Symbol;Ac... [more]
Dgkg4.251e-843.86diacylglycerol kinase, gamma [Source:MGI Symbol;Ac... [more]
Dgkb2.791e-16355.79diacylglycerol kinase, beta [Source:MGI Symbol;Acc... [more]
Dgkb2.791e-16355.79diacylglycerol kinase, beta [Source:MGI Symbol;Acc... [more]
Dgka6.378e-13048.02diacylglycerol kinase, alpha [Source:MGI Symbol;Ac... [more]
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BLAST of Diacylglycerol kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q03603|DGK3_CAEEL0.000e+046.44Probable diacylglycerol kinase 3 OS=Caenorhabditis... [more]
sp|P49620|DGKG_RAT3.173e-746.05Diacylglycerol kinase gamma OS=Rattus norvegicus O... [more]
sp|Q91WG7|DGKG_MOUSE2.792e-745.50Diacylglycerol kinase gamma OS=Mus musculus OX=100... [more]
sp|Q6NS52|DGKB_MOUSE1.962e-16255.79Diacylglycerol kinase beta OS=Mus musculus OX=1009... [more]
sp|Q9Y6T7|DGKB_HUMAN1.404e-16155.38Diacylglycerol kinase beta OS=Homo sapiens OX=9606... [more]
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BLAST of Diacylglycerol kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V4A5240.000e+053.54Diacylglycerol kinase OS=Lottia gigantea OX=225164... [more]
A0A1D1URY80.000e+053.72Diacylglycerol kinase OS=Ramazzottius varieornatus... [more]
A0A1W0WBN00.000e+053.08Diacylglycerol kinase OS=Hypsibius dujardini OX=23... [more]
A0A1W0WBI20.000e+053.08Diacylglycerol kinase OS=Hypsibius dujardini OX=23... [more]
R7TZB07.390e-754.58Diacylglycerol kinase OS=Capitella teleta OX=28390... [more]
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BLAST of Diacylglycerol kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dgkaa0.000e+043.76diacylglycerol kinase, alpha a [Source:ZFIN;Acc:ZD... [more]
dgkab1.210e-11845.47diacylglycerol kinase beta [Source:NCBI gene;Acc:1... [more]
dgkg1.780e-9455.21diacylglycerol kinase, gamma [Source:ZFIN;Acc:ZDB-... [more]
dgkzb2.265e-8131.38diacylglycerol kinase zeta [Source:NCBI gene;Acc:1... [more]
dgkzb1.198e-8031.38diacylglycerol kinase zeta [Source:NCBI gene;Acc:1... [more]
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BLAST of Diacylglycerol kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000002929.12.463e-15854.29pep scaffold:Pmarinus_7.0:GL476935:158222:277059:-... [more]
ENSPMAT00000009725.11.542e-15553.74pep scaffold:Pmarinus_7.0:GL478043:6842:50807:-1 g... [more]
dgkzb4.388e-7931.52diacylglycerol kinase, zeta b [Source:ZFIN;Acc:ZDB... [more]
ENSPMAT00000007078.12.964e-6736.80pep scaffold:Pmarinus_7.0:GL477921:24257:38296:1 g... [more]
ENSPMAT00000007068.12.310e-6636.87pep scaffold:Pmarinus_7.0:GL477921:19741:38299:1 g... [more]
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BLAST of Diacylglycerol kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Diacylglycerol kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO425100.000e+046.31Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO382876.595e-4534.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO345912.705e-3351.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO484352.966e-2932.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO406845.990e-2746.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Diacylglycerol kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dgkb8.751e-16654.36diacylglycerol kinase beta [Source:NCBI gene;Acc:1... [more]
dgkg4.735e-13950.32diacylglycerol kinase beta [Source:NCBI gene;Acc:1... [more]
dgkb1.929e-13548.50diacylglycerol kinase beta [Source:NCBI gene;Acc:1... [more]
dgkaa1.253e-13248.68diacylglycerol kinase alpha [Source:NCBI gene;Acc:... [more]
dgkab1.474e-11542.98diacylglycerol kinase beta [Source:NCBI gene;Acc:1... [more]
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BLAST of Diacylglycerol kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000075921.10.000e+098.45SMESG000075921.1[more]
SMESG000075921.10.000e+098.35SMESG000075921.1[more]
SMESG000051710.13.316e-8232.14SMESG000051710.1[more]
SMESG000051710.14.488e-8232.14SMESG000051710.1[more]
SMESG000051710.14.699e-8031.70SMESG000051710.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009837 ID=SMED30009837|Name=Diacylglycerol kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=3344bp
GAACAGAACATGTGCTGGAATTTTCTTGTTCTAATTTAGTTTACGATGAA
ATTGTGATGTCTAATTTAATTCATTAAAGGAATGACATTTTCTGCGTCTT
GTAGTCATTTCACTTTTGAAGACTTCAATCAATTACAAGACTTTATTTCA
GATGCTATACATTCTCTGCAAGATGTAGTCCAAAAGGAATTCAAACCAGG
ATCAAAATTAGCTCAATATGATCCAGAAAAACCAATTGATCATAATGGAT
ACTGCCAATTTATTACTGCTTACTTGGGAGAAAACTTCCCCCAGTCAATC
CGGGATAAACTATTTCTTTCATTTATGAGACGTCATTCCAACCTGGAGAT
TTATAACTGTGAACCAAATAAGGCACCTACATTAAAATCGAAAATAAATG
AAGATAATGGTAAAAGGATAATTTACGAGAGTCGAATTCGAAACAATAGC
TTAAACGCCACTAGCACAAATGCAATACTGAGTAACCTTTCTGAAAGATT
AAGCGCTATTGCACATAGCTCCACAATTTTATCATCAAAATCTATTTTTT
CAACATATGTTTGCCACAATAACTTGCAATGTTGTCAATCATCAATTTCA
CATCAGAGATGTCGAACTTTGCCTCTTAATCATATCGATTCTACCTTTAA
TGTTTTCGGAAAAAAGTATAAGTCAAACTTAGGTTCCGCTCATAGTCATC
CCTCTCTTGTTACTCATACTATTCAGCCTATATTTCCCAACGAGGACACC
CCGATTCATCATTCACGTTCGTCCTCTAAAAAGTCAGATAAAAAAATTGA
AAATGAAATCGTTTGCGAAAAATCAGCTTTGACTTTATCGAAGTCAAATA
ACTTAGATATTGTTAATAGGCCTCTAACGGACCAAGACAAAATTGATATG
AAGGATTTGTTATGTTACTTGAGTTTACTGGAGTCTGGAAAACCTGAAGA
CAAATTGGAGTTTATGTTTAGATTATATGACACCGATGGAAACGGAGTTT
TGGATAAATCTGAAGTTGAATGCATAATTAGCCAGATGATGAATGTTGCT
GGATATCTAAATTGGGATACGGAAGAATTATTGCCGATCCTACAGCAAAT
GATGAATGAAATTGACTATGACAATGATGGTGAAGTAACATTGGAAGAGT
GGAAAAGAGGTGGAATGACAAATATACCATTACTTGTATTGCTCGGTTTG
GATACTAATGTGAGAGACGATGGAAAACATGTTTGGAAATTGAAGCATTT
TTCTAAATCTACTCATTGCAGCCTTTGCCACAAAATTCTAGTAAGATTTG
GTCGGCAAGGCCTCTGTTGTGTATTTTGCTTGTACACTGTGCACGAAAGA
TGTGTTCAAAATGCTCCAGCTTCTTGTATTCACACATATACCAAAACTAA
AAAAAGTGATCAGTTATTAGAACATCATTGGATTGAAAATCATTATCCAG
CAAAATGTATCAAATGCAAAAAATCTACAAAATTAGGGGCCAAATCCGGA
TTTCACTGTAGGTGGTGTAAAAGAACCATTCATAAATGTTGTATGGAAAG
TTGGAGACAAACGTGTGATTTAGGGGATAACAAGGAACATATTCTTCCTC
CGATTTGTATATACCCAAGTGTTTTGGAAAGAAAACAAACAGCAAAAAAC
AAAGACTGTTTGAATATTGAAAAAACAAGTGAAAGCATGAATCTAGCTGA
TAGTGCTGCGGATGAAGTGGATTGTCAAGAAAATAACTGTGGAAATAATA
GCAATTCTGAAAATATTTTACCACAGTCATCTAATTCGCAATGTTTCATA
CAAGAGCAGGGTTTAAATCATAATCAAATTTCTTCATTTCAAATATGTGC
GTTACCTGACACTAAACCACTGCTAGTTTTTATCAATCCAAAATCTGGTG
GGAAACAAGGAGCAAGGATTATGACAAAATTTCAATATATATTAAATCCT
CGTCAAGTTTATGATCTTGTTAAAGATAAAGGACCAAATAATGGATTACA
GTTCTTTAGAGATGTTAAAGACTTTAGAATTTTAATTTGTGGAGGTGACG
GTACTGTTGGCTGGCTATTGGAAGCTATAGATAAATATGAATGGAATAAC
AACCGGCCTTCTGTTGCCATTTTACCCCTGGGAACTGGAAATGATTTGGC
TCGATGTTTAAGATGGGGAGGGGGATATGAAGGAGAAAAATTGGATAAAA
TTCTAGCGAAGATAATACTCGGTACTGTAGTAATGCTTGATAGATGGCAG
ATCACTTGTTCAGAACCAGCACCAAGGCCAAGAAGGGAATCAAAGACATC
CAATGGCTGTGGGAGAACTGGGTGCACTGGATATTGTTCTCGAAATCGGG
GAGTTAATCAAGATTCCAGCAGTCAAAATGATTCTGGAATTAAAGAAGAT
AGTCAAATAGATTGTTGCGAAGAAGGTGATTTAGATTCTGATGTCCTAAG
TGAAGATTCAATTTATAGTGATGAAATGCCGTGCAATATTATCAACAATT
ATTTCAGTATTGGAGTAGATGCCAGCATTGCTCACAAATTTCATGTTTTA
CGTGAAAAACACCCAGAAAAATTTAACAGTCGAATGAGAAATCGTTTTTT
TTACATGGAAGTCGGAACTTCCGAAGTATTAACTGCAACTTGCAAACATC
TTCACGAATACATTGATATTATTTGTGATGGAGTAGTTTTAGATTTGAGA
AAAGGTGCTTATTTAGAGGGAATAGCAATTTTAAACATACCATCAGTATA
TGGAGGTAAAAATTTATGGGGTCCAGTAATTTCTCCCTCAAAAATTAAGA
AAAATCAACTAAACAAACCAAAAAAGAAAAAGTCTGATTCAGGAAGATTT
GAATCCCGAGAACTTTCAAGAGAATCGACATTTGAAAATCTTGCTACTGC
TGTTCAAGATATGGGAGATAATTTACTTGAAGTTGTTGGTCTGGAGAATG
CTATGCATGCTGGACTGGTCGCAGGAGGTTTTCGAAGTTCTGGTAAAAGA
CTAGCTCAGTGTAGAGAAGTAGTTATACGTACTCAAAGATGCTTCCCAAT
GCAGATTGATGGAGAACCATGGATTCAAGCGGCTTGCACTATATCCATAA
CATATAAAAATCAAGTCCCGATGATTATGGCTCCTCCTTTAAAGAAGTTT
GGTTTGAGAAACATGTTTTCCCGTTAACAAAATTATGATCTCTGAGTATT
TTATATCATATAGAATCATAATTTTGATTATATTAAATTTTTAATATCGA
GATGAATACTTTTTGATGCAGTTATATTTTAAAAATTCATATTATAACTT
TAATTTCATTTTTTTTTGAATAAATGATAAACATTGTAAAAAAA
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protein sequence of SMED30009837-orf-1

>SMED30009837-orf-1 ID=SMED30009837-orf-1|Name=SMED30009837-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1032bp
MTFSASCSHFTFEDFNQLQDFISDAIHSLQDVVQKEFKPGSKLAQYDPEK
PIDHNGYCQFITAYLGENFPQSIRDKLFLSFMRRHSNLEIYNCEPNKAPT
LKSKINEDNGKRIIYESRIRNNSLNATSTNAILSNLSERLSAIAHSSTIL
SSKSIFSTYVCHNNLQCCQSSISHQRCRTLPLNHIDSTFNVFGKKYKSNL
GSAHSHPSLVTHTIQPIFPNEDTPIHHSRSSSKKSDKKIENEIVCEKSAL
TLSKSNNLDIVNRPLTDQDKIDMKDLLCYLSLLESGKPEDKLEFMFRLYD
TDGNGVLDKSEVECIISQMMNVAGYLNWDTEELLPILQQMMNEIDYDNDG
EVTLEEWKRGGMTNIPLLVLLGLDTNVRDDGKHVWKLKHFSKSTHCSLCH
KILVRFGRQGLCCVFCLYTVHERCVQNAPASCIHTYTKTKKSDQLLEHHW
IENHYPAKCIKCKKSTKLGAKSGFHCRWCKRTIHKCCMESWRQTCDLGDN
KEHILPPICIYPSVLERKQTAKNKDCLNIEKTSESMNLADSAADEVDCQE
NNCGNNSNSENILPQSSNSQCFIQEQGLNHNQISSFQICALPDTKPLLVF
INPKSGGKQGARIMTKFQYILNPRQVYDLVKDKGPNNGLQFFRDVKDFRI
LICGGDGTVGWLLEAIDKYEWNNNRPSVAILPLGTGNDLARCLRWGGGYE
GEKLDKILAKIILGTVVMLDRWQITCSEPAPRPRRESKTSNGCGRTGCTG
YCSRNRGVNQDSSSQNDSGIKEDSQIDCCEEGDLDSDVLSEDSIYSDEMP
CNIINNYFSIGVDASIAHKFHVLREKHPEKFNSRMRNRFFYMEVGTSEVL
TATCKHLHEYIDIICDGVVLDLRKGAYLEGIAILNIPSVYGGKNLWGPVI
SPSKIKKNQLNKPKKKKSDSGRFESRELSRESTFENLATAVQDMGDNLLE
VVGLENAMHAGLVAGGFRSSGKRLAQCREVVIRTQRCFPMQIDGEPWIQA
ACTISITYKNQVPMIMAPPLKKFGLRNMFSR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003951NAD+ kinase activity
GO:0005509calcium ion binding
GO:0016301kinase activity
GO:0004143diacylglycerol kinase activity
GO:0000166nucleotide binding
GO:0005524ATP binding
GO:0016740transferase activity
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
GO:0007205protein kinase C-activating G-protein coupled receptor signaling pathway
GO:0035556intracellular signal transduction
GO:0016310phosphorylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR038199DGK_typeI_N_sf
IPR017438ATP-NAD_kinase_N
IPR037607DGK
IPR002048EF_hand_dom
IPR001206Diacylglycerol_kinase_cat_dom
IPR000756Diacylglycerol_kin_accessory
IPR002219PE/DAG-bd
IPR029477DAG_kinase_typeI_N
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR016064NAD/diacylglycerol_kinase_sf
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainSMARTSM00109c1_12coord: 448..495
e-value: 0.019
score: 21.9
coord: 383..432
e-value: 2.6E-7
score: 40.3
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPFAMPF00130C1_1coord: 383..433
e-value: 1.4E-9
score: 37.6
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS00479ZF_DAG_PE_1coord: 383..432
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 382..432
score: 13.093
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 447..495
score: 10.959
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainCDDcd00029C1coord: 383..432
e-value: 9.69963E-12
score: 58.6576
IPR002048EF-hand domainSMARTSM00054efh_1coord: 336..364
e-value: 0.015
score: 24.5
coord: 291..319
e-value: 0.0023
score: 27.2
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 290..356
e-value: 5.0E-9
score: 36.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 332..367
score: 8.962
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 287..322
score: 13.174
IPR002048EF-hand domainCDDcd00051EFhcoord: 266..311
e-value: 2.84773E-6
score: 43.6905
IPR002048EF-hand domainCDDcd00051EFhcoord: 291..360
e-value: 6.56024E-15
score: 67.9581
IPR000756Diacylglycerol kinase, accessory domainSMARTSM00045dagk_c4b_2coord: 803..996
e-value: 2.2E-81
score: 286.4
IPR000756Diacylglycerol kinase, accessory domainPFAMPF00609DAGK_acccoord: 804..996
e-value: 2.9E-45
score: 154.5
IPR001206Diacylglycerol kinase, catalytic domainSMARTSM00046dagk_c4a_7coord: 596..722
e-value: 3.5E-61
score: 219.2
IPR001206Diacylglycerol kinase, catalytic domainPFAMPF00781DAGK_catcoord: 596..709
e-value: 5.8E-28
score: 97.0
IPR001206Diacylglycerol kinase, catalytic domainPROSITEPS50146DAGKcoord: 592..729
score: 45.668
IPR038199DGK type I, N-terminal domain superfamilyGENE3DG3DSA:1.10.238.110coord: 236..292
e-value: 1.2E-7
score: 33.7
coord: 5..101
e-value: 7.4E-14
score: 53.7
NoneNo IPR availableGENE3DG3DSA:3.30.60.20coord: 375..449
e-value: 1.2E-11
score: 46.1
NoneNo IPR availableGENE3DG3DSA:3.30.60.20coord: 450..496
e-value: 4.1E-5
score: 25.2
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 293..366
e-value: 1.8E-15
score: 59.1
NoneNo IPR availablePANTHERPTHR11255:SF48DIACYLGLYCEROL KINASE 1coord: 220..1022
NoneNo IPR availableSUPERFAMILYSSF57889Cysteine-rich domaincoord: 439..498
NoneNo IPR availableSUPERFAMILYSSF57889Cysteine-rich domaincoord: 371..435
IPR029477Diacylglycerol kinase type I, N-terminalPFAMPF14513DAG_kinase_Ncoord: 10..168
e-value: 9.7E-18
score: 64.9
IPR017438Inorganic polyphosphate/ATP-NAD kinase, N-terminalGENE3DG3DSA:3.40.50.10330coord: 593..715
e-value: 6.6E-14
score: 53.4
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 220..1022
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 300..312
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 345..357
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 8..295
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 267..372
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILYSSF111331NAD kinase/diacylglycerol kinase-likecoord: 595..1017