SMED30009798
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009798 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8Note: Hover over icons to view figure legend
Alignments
SMED30009798 aligns in the following genomic locations:
Homology
BLAST of SMED30009798 vs. TrEMBL
Match: A0A2G8KM90 (Putative ubiquitin-conjugating enzyme E2 U-like OS=Stichopus japonicus OX=307972 GN=BSL78_13973 PE=3 SV=1) HSP 1 Score: 65.4698 bits (158), Expect = 1.723e-8 Identity = 42/149 (28.19%), Postives = 72/149 (48.32%), Query Frame = 2 Query: 347 EISFLDYLLTWNGIATSKPNKDSENLYLNQIRNQTKLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGHNENYRIDKFRSKALEYQSNLKMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI 793 ++SF +YL TW GIATSKP + +N L I+ +KL + H G+ L +++Q ++HN +YGNF KK + + + L+ S +K I ++S + +E + +++DL+ WT L D+ Sbjct: 188 KVSFDEYLTTWTGIATSKPQINMKNPLLEAIKRDSKLQAAHFGLTLEDLQEQMKKQLNEHNAIMYGNFGKK------------QKTQDAQERRLDQISKMKKIYLPRRSAPPP-----STAAQSESGEPWDGDVEDLVAWTNELDEDDL 319
BLAST of SMED30009798 vs. TrEMBL
Match: V3Z724 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_235267 PE=4 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 1.938e-8 Identity = 45/152 (29.61%), Postives = 69/152 (45.39%), Query Frame = 2 Query: 350 ISFLDYLLTWNGIATSKPNKDSENLYLNQIRNQTKLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGHNENYRIDKFRSKALEYQSNLKMIIHIKQSE-KSSKHFIENNLFHNE---DESADEAEMKDLMEWTENLKTCDI 793 +SF DY TW GIATSK + N L+++R + +L H + I++Q ++H M +YG F KP + D+ +K Q K+ + + S +S+ + H E E E+ DL+EWT NL T I Sbjct: 57 LSFEDYYTTWCGIATSKSKEVDTNPLLDKLREKPELQKTHMALRPEDIEEQIKKQIEEHTMLMYGKFDSKPTVQ-----------DEKEAKLARLQKMKKIYLPPRVSPSPASQDIVLGGSLHTSLHGKEEPWEKEVDDLVEWTNNLDTTTI 197
BLAST of SMED30009798 vs. TrEMBL
Match: A0A433T1T1 (UBIQUITIN_CONJUGAT_2 domain-containing protein OS=Elysia chlorotica OX=188477 GN=EGW08_016726 PE=3 SV=1) HSP 1 Score: 62.3882 bits (150), Expect = 1.789e-7 Identity = 46/148 (31.08%), Postives = 71/148 (47.97%), Query Frame = 2 Query: 347 EISFLDYLLTWNGIATSKPNKDSENLYLNQIRNQTKLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQL-SFNDGHNENY-RIDKFRSKALEYQSNLKMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKT 784 ++SF DY TW GIATSK D+ N +L IR KL IH G+ +++Q ++H++ +YG F +P + S D N R+ K + + I I +S + + +ES E E+ DL+ WT NL + Sbjct: 197 KLSFDDYHTTWCGIATSKTRPDAINPFLETIRGNPKLQEIHLGVPREELEDQMKKQMEEHSILMYGKFKSQPSVESVRDAKIANLERMKKIYFPPRQSPLPPREDIDIGKSLPALQ----------ANESNWEKEVDDLVNWTNNLDS 334
BLAST of SMED30009798 vs. TrEMBL
Match: C3YQR1 (UBIQUITIN_CONJUGAT_2 domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_130394 PE=3 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 2.751e-7 Identity = 65/260 (25.00%), Postives = 110/260 (42.31%), Query Frame = 2 Query: 11 PFHPNSKYSY*YFPQLKYPSYSVDSKTGQIGLEMNKFKQE--NYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFXXXXXXXXXXXXXXXXEISFLDYLLTWNGIATSKPNKDSENLYLNQIRNQTKLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGHNENYRIDKFRSKALEYQSNLKMIIHIKQSEKSSKHFIENNLFHNEDESAD--EAEMKDLMEWTENL 778 PFHPN V S TG+ ++ QE + L+ S LL NP +E+ + N A ++ ++N +Y +V + + K ++SF +Y TW+GIATSK +N L IR+ S H+G+ + +++Q ++HN +YG F + G ++ R K ++ K+ + + +E S+ + F ES + E E+ DL+ WT NL Sbjct: 79 PFHPN-----------------VHSITGRPCIDYLDNIQEWKESYSLVHVLLSIQTLLSNPVVEDAV-NDDAARMLVTNPDRYLEMV----RSCVDASKRVHAPAAKTAGIKTRKVSFDEYHTTWSGIATSKATPAMKNPLLESIRDDPIRQSSHYGLTVEQLQDQMKRQLEEHNALMYGKFV-------DHGKTDDQR----EQKLMKINQMRKVYMSKRITEPSTPTPSDTKEFAPPKESEEPWEKEVDDLVAWTHNL 305
BLAST of SMED30009798 vs. TrEMBL
Match: K1QM65 (Ubiquitin-conjugating enzyme E2 U OS=Crassostrea gigas OX=29159 GN=CGI_10008530 PE=3 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 5.872e-7 Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 2 Query: 350 ISFLDYLLTWNGIATSKPNKDSENLYLNQIRNQTKLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKK 559 +SF DY +TW+GIATSKP ++ N L I+++ L H GI +++Q ++HN +YGNF K Sbjct: 195 LSFDDYFMTWSGIATSKPTIETPNPLLEAIKDKPGLQRAHLGIPQEEIQDQMKRQLEEHNALMYGNFKMK 264
BLAST of SMED30009798 vs. Planmine SMEST
Match: SMESG000051529.1 (SMESG000051529.1) HSP 1 Score: 444.891 bits (1143), Expect = 9.487e-159 Identity = 238/262 (90.84%), Postives = 242/262 (92.37%), Query Frame = 2 Query: 8 PPFHPNSKYSY*YFPQLKYPSYSVDSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFXXXXXXXXXXXXXXXXEISFLDYLLTWNGIATSKPNKDSENLYLNQIRNQTKLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGHNENYRIDKFRSKALEYQSNLKMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI 793 PPFHPN +DSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFVSARSSVRNARRRKRNEISFLDYLLTWNGIATSKPN+DSENLYLNQIRNQ +LLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDG NENYRIDK RSKALEYQSN+KMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI Sbjct: 89 PPFHPN-----------------IDSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFVSARSSVRNARRRKRNEISFLDYLLTWNGIATSKPNEDSENLYLNQIRNQKQLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGQNENYRIDKLRSKALEYQSNMKMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI 333
BLAST of SMED30009798 vs. Planmine SMEST
Match: SMESG000051529.1 (SMESG000051529.1) HSP 1 Score: 444.506 bits (1142), Expect = 1.351e-158 Identity = 238/262 (90.84%), Postives = 242/262 (92.37%), Query Frame = 2 Query: 8 PPFHPNSKYSY*YFPQLKYPSYSVDSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFXXXXXXXXXXXXXXXXEISFLDYLLTWNGIATSKPNKDSENLYLNQIRNQTKLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGHNENYRIDKFRSKALEYQSNLKMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI 793 PPFHPN +DSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFVSARSSVRNARRRKRNEISFLDYLLTWNGIATSKPN+DSENLYLNQIRNQ +LLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDG NENYRIDK RSKALEYQSN+KMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI Sbjct: 86 PPFHPN-----------------IDSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFVSARSSVRNARRRKRNEISFLDYLLTWNGIATSKPNEDSENLYLNQIRNQKQLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGQNENYRIDKLRSKALEYQSNMKMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI 330
BLAST of SMED30009798 vs. Planmine SMEST
Match: SMESG000051529.1 (SMESG000051529.1) HSP 1 Score: 443.736 bits (1140), Expect = 1.739e-158 Identity = 238/262 (90.84%), Postives = 242/262 (92.37%), Query Frame = 2 Query: 8 PPFHPNSKYSY*YFPQLKYPSYSVDSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFXXXXXXXXXXXXXXXXEISFLDYLLTWNGIATSKPNKDSENLYLNQIRNQTKLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGHNENYRIDKFRSKALEYQSNLKMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI 793 PPFHPN +DSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFVSARSSVRNARRRKRNEISFLDYLLTWNGIATSKPN+DSENLYLNQIRNQ +LLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDG NENYRIDK RSKALEYQSN+KMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI Sbjct: 78 PPFHPN-----------------IDSKTGQIGLEMNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERLVVQGKLNQESTFVSARSSVRNARRRKRNEISFLDYLLTWNGIATSKPNEDSENLYLNQIRNQKQLLSIHHGIDLHSAGSLIRQQEDKHNMYLYGNFAKKPQLSFNDGQNENYRIDKLRSKALEYQSNMKMIIHIKQSEKSSKHFIENNLFHNEDESADEAEMKDLMEWTENLKTCDI 322 The following BLAST results are available for this feature:
BLAST of SMED30009798 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30009798 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30009798 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30009798 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30009798 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30009798 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009798 ID=SMED30009798|Name=SMED30009798|organism=Schmidtea mediterranea sexual|type=transcript|length=812bpback to top protein sequence of SMED30009798-orf-1 >SMED30009798-orf-1 ID=SMED30009798-orf-1|Name=SMED30009798-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=229bp MNKFKQENYFDLLLFCQSTIDLLKNPNLENNISNILAMKLFLSNRQQYERback to top Annotated Terms
The following terms have been associated with this transcript:
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