Laminin subunit alpha-2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009787 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 19
Homology
BLAST of Laminin subunit alpha-2 vs. Ensembl Human
Match: LAMA1 (laminin subunit alpha 1 [Source:HGNC Symbol;Acc:HGNC:6481]) HSP 1 Score: 754.592 bits (1947), Expect = 0.000e+0 Identity = 448/1182 (37.90%), Postives = 632/1182 (53.47%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT--HK--GYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNG--EDIGDQK-PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALP----KFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQ-STLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQ--RQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITLSRGEVH-LALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK--KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC------YNGQVCNNE----------GICVCPPNTIGNLCNKCSEDSWDF--NETIGCKKCNCYINGSYSNICNKI 3550
L NA I+++ATCG GPE+FC+LV+ + + + NP C +CD + N +RHPI A D N WWQSPS+ G +Y V +T+DL+QV+Q+ Y+++K ++PRPGNWILERSLDG + PW ++A + C YN+ R+ P P+ ADDE+ CT+YYS+ L GEI SLINGR S + LS K L+ F SARYIR R + TL ADLMT H+ L+ + RYYYS+++I +GG CIC HA C + +C C+HNTCG +C CCP ++Q+PW+P V CE CNC+ K+ +C Y+++VA + S+N G+ GGGVCINC +NT GINCE CI G+YRP V E PC PC C +G++ S C+ + D+ + K PG C CK+GY G CD C +GY+ YP C+ C C+ GS S C PC CK +V C CK G++NL NP GC C+CFG+++ CSSLS +++ + GW +T + +P ++ N+ + +R YYW AP YLGN +T++G +Y V ++ T+ +L+ DVIIK L S E LQ + ++ + +++ ++ L V LLI+A Y S + ++ L +++L + + L +E C CP G+ G SCESC SGY RV+ +L+ G C+ C C+ H+ C+ ++G C C HNT G HCE C G+YG+ C+ C CP + C + VC C SG C+RC +GYYGNP SC C C+ N + CD VTGEC +C NT G C+ C GFYGDA+T K+C+ C+C GS S C TG CDC+ N +QCD C G Y D C PCNC + G +S C T GQC C+ V G RC++C G++ Y + C C C PH + CD G+C C + C++CE+ H+ ++ C CNC GS + +CD+ TG+C+C R C +C +GY C PC +G CN E G C C N G CN+C E ++ + +GC C C S++C+++
Sbjct: 29 LASNAHISTNATCGEKGPEMFCKLVEHVPGRPVRNPQ----CRICDGNSANPRERHPISHAI-DGTNNWWQSPSIQNGREYHWVTITLDLRQVFQVAYVIIKAANAPRPGNWILERSLDGTTFSPWQYYAVSDSECLSRYNITPRRGP---------PTYRADDEVICTSYYSRLVPLEHGEIHTSLINGRPSA---DDLSPK-----LLEFTSARYIRLRLQRIRTLNADLMTLSHREPKELDPIVTRRYYYSIKDISVGGMCICYGHASSCP--WDETTKKLQCQCEHNTCGESCNRCCPGYHQQPWRPGTVSSGNTCEACNCHNKAKDCYYDESVAKQKKSLNTAGQFRGGGVCINCLQNTMGINCETCIDGYYRPHKVSPYEDEPCRPCNCDPVGSLSSVCIKDDLHSDLHNGKQPGQCPCKEGYTGEKCDRCQLGYKDYPT--CVSCGCNPVGSA--SDEPCTGPCVCKENVEG-KACDRCKPGFYNLKEKNPRGCSECFCFGVSDVCSSLSWPVGQVNSMSGWLVTDLISPR-KIPSQQDALGGRHQVSINNTAVMQRL--APKYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETVDSNLMSHADVIIKGNGLTL--STQAEGLSLQPYEEYLNVVRLVPENFQDFHSKRQIDRDQLMTVLANVTHLLIRANYNSAKMALYRLESVSLDIASSNAIDLVVAADVEHCECPQGYTGTSCESCLSGYYRVDGILFGGICQPCECHGHAAECN-VHGVCIACAHNTTGVHCEQCLPGFYGEPSRGTPGDCQPCACPLTIASNNFSPTCHLNDGDEVVCDWCAPGYSGAWCERCADGYYGNPTVPGESCVPCDCSGNVDPSEAGHCDSVTGECLKCLGNTDGAHCERCADGFYGDAVTAKNCRACECHVKGSHSAVCHLETGLCDCKPNVTGQQCDQCLHGYYGLDSGHGCRPCNCSVAGSVSDG--C-TDEGQCHCVPGVAGKRCDRCAHGFYAYQDGSCTPCDC-PHTQNT-CDPETGECVCPPHTQGVKCEECEDGHWGYDAEVGCQACNCSLVGSTHHRCDVVTGHCQCKSKFGGRACDQCSLGYRDF-----PDCVPCDCDLRGTSGDACNLEQGLCGCVEETGACPCKENVFGPQCNECREGTFALRADNPLGCSPCFC---SGLSHLCSEL 1162 HSP 2 Score: 397.512 bits (1020), Expect = 6.053e-110 Identity = 410/1680 (24.40%), Postives = 683/1680 (40.65%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPKKKIS--CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCD-SLGST--SENCDKLTGK---CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCS-PHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNE------LTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYE----YHIFDEKN--SLESGKDYST--IHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDN------------KCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIK-IKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY-KNDKNYDQCEPCQCP-SAHYNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKINISIQMSSELL--NSINKFYKLK-----NEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKV---LEDLRSTFTDINETISGHEQL--------GISNELIKNATAYLET--IKNIYKEMPNITLAASVLKDVLKYVDELNQ-------IIKYTQSHIQSDQINNLKILKNKTIMADE-----------INTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVR-EIEDLMKNLSSNHLV----------SKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFL----HNAEQAKEAYSNITRDILAVDYAINQTKKEL--------DFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDL-----VNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLP---TSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGK-WHMVIRSISDGRTEQVETSSI--TNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRN 7216
+C +G C+ C+ GYY C+ C+C+ + C+ V G C C++NT G C+ C GFYG+ C+ C C ++ S S C G CD C + C+ C G Y + + C+PC+C S C++ +G+C CL N +G CE+C +G++ NC+ C C + SA C L G C C NV + CD+C + +Y ++ C PCNC GS++ C E G C C VA + C +C G++ C C + C+ E G CVCPP+T G C +C + W ++ +GC+ CNC + GS + C+ ++G C CK+ F G CD+C+ GY FP+C PC C GT + L C + TG C CK+NV G +C CR+GTF GC CFC G+S C + ++ ++ + + N G Y L+A R+ P Y + + F L + + +V+ LD K GR I +Q ++M++ G+R E + +Y N + +K + ED FM L ++ + +K Y + I I + + E + + +E C+C T G CQ+ GY++ LP +GS R ++ C+CN +S CD TG C NC N G+ C +C G+Y K + C C CP S +F+ C +E + +C C +GY GK CE C +YGNP G C+ CDCN GS +C+ G C C G G++CD+C P +++ + C SC C LL +D + + V++ +P + N N + + LL N K+K E N + +M ++ E++ + +E L+ + T+I E + ++ L + +N T+ LE I++ + N TL +D+L + E Q ++K SH+ S N LK + A+ +N N +E+ + ++E L LI + + + Q D ++ ++ L+ QD+ L S +R I+DL+ ++S + V ++ + D L +E N S N +N + E A++A+ +T L + ++ K + + N + + + L+E + + EN I ++ + L + IP G D + L+ + + ++Q +LN + R +L++ D+ L V + N + D K +K L E L + +L+ A + I+ V R + Y P ++N +L +K Q ++ L Y S + A + R + F +D G+ +E ++N + + G + ++ +S + +TS T N+L + T +FVGG ++ +S ++ +KGCL L GL + E +CR
Sbjct: 710 ECPQGYTGTSCESCLSGYYRVDGILFGGICQPCECHGHAAECN-VHGVCIACAHNTTGVHCEQCLPGFYGEPSRGTPGDCQPCACPLTIASNNFSPTCHLNDGDEVVCDWCAPGYSGAWCERCADGYYGNPTVPGESCVPCDCSGNVDPSEAGHCDSVTGECLKCLGNTDGAHCERCADGFYGDAVTAKNCRACECHVKGSHSAVCHLETGLCDCKPNVTGQQCDQCLHGYYGLDSGHGCRPCNCSVAGSVSDGCTDE-GQCHCVPGVAGKRCDRCAHGFYAYQDGSCTPCDCPHTQNTCDPETGECVCPPHTQGVKCEECEDGHWGYDAEVGCQACNCSLVGSTHHRCDVVTGHCQCKSKFGGRACDQCSLGYRDFPDCVPCDCDLRGTSGDACNLEQGLCGCVEETGACPCKENVFGPQCNECREGTFALRADNPLGCSPCFCSGLSHLCSELEDYVRTPVTLGSDQPLLRVVSQSNLRGTTEGVYYQAPDFLLDAATVRQH----IRAEPFYWRLPQQFQGDQLMAYGGKLKYSVAFYSLDGVGTSNFEPQVLIKGGR-IRKQV-IYMDAPAPENGVR-----------------QEQEVAMRENFWKYFNSVSEKPVTRED------------FMSVLSDIEYILIKASYGQGLQQSRISDISMEVGRKAEKLHPEEEVASLLENCVCPPGTVGFSCQDCAPGYHRG--KLP----AGSDRGPRPLVAPCVPCSCNNHSDTCDPNTGKCLNCGDNTAGDHCDVCTSGYYGKVTGSASDCALCACPHSPPASFSPTCV-LEGDHDFRCDACLLGYEGKHCERCSSSYYGNPQTPGGSCQKCDCNPHGSVHGDCDRTSGQCVCRLGASGLRCDECEPRHILMETDCVSCDDECVGVLLNDLDEIGDAVLSLNLTGIIPVPYGILSNLENTTKYLQESLLKENMQKDLGKIKLEGVAEETDNLQKKLTRMLASTQKVNRATERIFKESQDLAIAIERLQMSITEIMEKTTLNQTLDEDFLLPNSTLQNMQQNGTSLLEIMQIRDFTQLHQNATLELKAAEDLLSQIQENYQKPLEELEVLKEAASHVLSKHNNELKAAEALVREAEAKMQESNHLLLMVNANLREFSDKKLHVQEEQNLTSELIVQG----RGLIDAAAAQTDAVQDALEHLE----DHQDKLLLWSAKIRHHIDDLVMHMSQRNAVDLVYRAEDHAAEFQRLADVLYSGLENIRNVSLNATSAAYVHYNIQSLIEESEELARDAHRTVTETSLLSESLVSNGKAAVQRSSRFLKEGNNLSRKLPGIALELSELRNKTNRFQENAVEITRQTNESLLILR------------------AIPKGIRDKGAKTKELATSASQSAVSTLRDVAGLSQELLNTSASLSRVNTTLRETHQLLQ--DSTMATLLAGRKVKDVEIQANLLFDRLKPLKMLEENLSRNLSEIKLLISQARKQAASIKVAVSADRDCIRAYQPQISSTNYNTLTLNVKTQEPDN----LLFYLGSSTASDFLAVEMRR-GRVAFLWDLGSGSTRLEFPDFPIDDNRWHSIHVARFGNIGSLSVKEMSSNQKSPTKTSKSPGTANVLD---VNNSTLMFVGGL-GGQIKKSPAVK------------VTHFKGCLGEAFLNGKSIGLWNYIEREGKCRG 2296 HSP 3 Score: 229.565 bits (584), Expect = 4.700e-59 Identity = 192/690 (27.83%), Postives = 286/690 (41.45%), Query Frame = 2
Query: 914 YYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGS------VRTSFYRC---KPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFT-GEKIALPKFTSFQYL--TSENSYI---DERFNNPTV--------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR---LFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRG----EVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQ----------CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR-LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKL 2848
YY L + C C+ D + G +C+C G C+ C F C C+C N C G C+ C +T+G+ CE+C G W A C C C S V + D G C CK + G CD CS+GY +P +C+PC C + G+ + C C CK +V C C+ G F L + NP GC PC+C G+++ CS +L+ + T T G L + S L T+E Y D + TV +YW P + G+ + +YG +Y V + S + P V+IK G R ++ + ++++ + ++ WK +K T+E+F+S L ++ +LIKA Y + + +I++ G ++H + +E C CPPG G SC+ C GY R L G C C+C NHS+TCD G+C NC NTAG HC+ C SGYYG + + + C C CP + C ++ C CL G HC+RC YYGNP+ SC++C CN + CD +G+C C G C C+ + C CD + +G + D++
Sbjct: 939 YYGLDSGHGCRPCNCSVAGSVSDGCTDEG----QCHCVPGVAGKRCDRCAHGFYAYQ---DGSCTPCDCPHTQNTCDPET------------------GECV-CPPHTQGVKCEECEDGH---WGYDAE--VGCQACNC-------SLVGSTHHRCDVVTGHCQCKSKFGGRACDQCSLGYRDFP--DCVPCDCDLRGTSGDACNLEQGLCGCVEETGACPCKENVFGPQ-CNECREGTFALRADNPLGCSPCFCSGLSHLCS----------ELEDYVRTPVTLGSDQPLLRVVSQSNLRGTTEGVYYQAPDFLLDAATVRQHIRAEPFYWRLPQQFQGDQLMAYGGKLKYSVAFYSLDGVGTSNFE-PQVLIKGGRIRKQVIYMDAPAPENGVRQEQEVAMRENFWKYFNSVSEKPVTREDFMSVLSDIEYILIKASYGQGLQQSRISDISMEVGRKAEKLHPEEEVASLLENCVCPPGTVGFSCQDCAPGYHR--GKLPAGSDRGPRPLVAPCVPCSCNNHSDTCDPNTGKCLNCGDNTAGDHCDVCTSGYYGKV-TGSASDCALCACPHSPPASFSPTCVLEGDHDFRCDACLLGYEGKHCERCSSSYYGNPQTPGGSCQKCDCNPHGSVHGDCDRTSGQCVCRLGASGLRCDECEPRHI--LMETDCVSCDDECVGVLLNDLDEI 1571 HSP 4 Score: 168.318 bits (425), Expect = 1.826e-40 Identity = 191/712 (26.83%), Postives = 285/712 (40.03%), Query Frame = 2
Query: 3086 CLCSPHALSADCDLNNGK--CKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFN-----ETIGCKKCNCYINGSYSNIC-----------NKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI---------LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTA-VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILIN--LKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY--KNDKNYDQCEPCQCPS--AHYNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDC--NQQGSQSLNCNEF-GIC-QCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
C+C HA S D K C+C N C++C + W T C CNC + +C ++VA++ S G F G VC N C NT+G C C + + + E C+ CNC GS S++C K G C CK + G KCDRC GY +P C C C G+ S+E TG C CK+NV G C C+ G + GC CFCFGVS+ C ++ ++ +V + I I ++++L G+ +I+ A R + Y + + + L+ F + VS D + +V ++ NGL S + + E+ + V +Q D K + RD M L NVT L ++ + L +++ ++ + ++ AI V A VE C C G C++ +SGYY+ + IL + C C+G++ C+ V G C C N G C+ C GFY + C+PC CP A NF+ C + E+ C C GYSG CE C G+YGNP + G C CDC N S++ +C+ G C +C G C++CA + V K C++C
Sbjct: 270 CICYGHASSCPWDETTKKLQCQCEHNTCGESCNRCCPGYHQQPWRPGTVSSGNTCEACNCHN---------KAKDCYYDESVAKQKKSLNTAGQF-------------RGGGVCIN-----CLQNTMGINCETCIDGYYRPHKVSPYEDEPCRPCNCDPVGSLSSVCIKDDLHSDLHNGKQPGQCPCKEGYTGEKCDRCQLGYKDYPTCVSCGCNPVGSASDE-----PCTGPCVCKENVEGKACDRCKPGFYNLKEKNPRGCSECFCFGVSDVCSSLSWPVGQVNSMSGWLVTDLISPRKIPSQQDAL-GGRHQVSINNTAVMQR-------LAPKYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETVDSNLMSHADVIIKG-NGL-------TLSTQAEGLSLQPYEEYLNVVRLVPENFQ-DFHSK-----------RQIDRDQLMTVLANVTHLLIRANYNSAKMALYRLESVSLDIA-SSNAIDLVVAADVEHCECPQGYTGTSCESCLSGYYRV----------------DGILFGGICQPCECHGHAAECN-VHGVCIACAHNTTGVHCEQCLPGFYGEPSRGTPGDCQPCACPLTIASNNFSPTC-HLNDGDEVVCDWCAPGYSGAWCERCADGYYGNPTVPGESCVPCDCSGNVDPSEAGHCDSVTGECLKCLGNTDGAHCERCADGFYGDAVTAKNCRAC 902
BLAST of Laminin subunit alpha-2 vs. Ensembl Human
Match: LAMA2 (laminin subunit alpha 2 [Source:HGNC Symbol;Acc:HGNC:6482]) HSP 1 Score: 715.687 bits (1846), Expect = 0.000e+0 Identity = 433/1176 (36.82%), Postives = 614/1176 (52.21%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLM--THKG--YLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQ--KPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGS--------LIDSPDVIIKCGDFRLFHSVSIEDRR--LQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDE--MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQE-----SCDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYF-------------GLDVTGC----KKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW--DFNETIGCKKCNCY 3514
L NA IT++ATCG GPE++C+LV+ + Q + NP C +C+ + N +RHPI A D N WWQSPS+ G++Y V +T+DL+QV+QI Y+++K +SPRPGNWILERSLD Y PW + A T C YN++ R P PS DDE+ CT++YS+ L +GEI ISLINGR S + P+ L+ F SARYIR RF + TL ADLM HK ++ + RYYYS+++I +GG CIC HA C ++ + ++C C+HNTCG +C+ CCP F+Q+PW+ CE CNC+ K+ EC Y++ VA LS+N G+ GGGVCINC +NT GINCE C GF+RP V PC PC C +G++ E + PG C CK G+ GV+CD C+ GY YP +C C CS GS C PC CK +V D C CK+G+FNL N +GC+ C+C G++N+C S KI D+ GW LT G P+ S Q ++ N+ + R P YYW+AP PYLGN + + G + + +L+ + + +++ D+ I ++ S E L ++ I T + + ++EF++ L + ++L++ Y +I L+ V D +E C CPPG+ G SCESC +RRVN ++ G CE C C+ H+ +CD + GEC NC +T G +C+ C G+YG+ C+ C CP + C S +C C +G C+RC EGY+G P SC+ C+CN+N + SCD ++G C C T G C+LC G++GDA+ K+C+ C C++ GS SE C TG+C+CR N ++CD C +G + + C+PCNC G S+ F SGQC+C V G +C++C GYFN+ C C CS L +CD G+C C N I C KC + W + T C CNC GSL+ +C++ TG C C + C++C G++ G D T C KKC + G C C N G C++C + D +GC C C+
Sbjct: 46 LASNALITTNATCGEKGPEMYCKLVEHVPGQPVRNPQ----CRICNQNSSNPNQRHPITNA-IDGKNTWWQSPSIKNGIEYHYVTITLDLQQVFQIAYVIVKAANSPRPGNWILERSLDDVEYKPWQYHAVTDTECLTLYNIYPRTGP---------PSYAKDDEVICTSFYSKIHPLENGEIHISLINGRPSADDPS-------PE-LLEFTSARYIRLRFQRIRTLNADLMMFAHKDPREIDPIVTRRYYYSVKDISVGGMCICYGHARACP--LDPATNKSRCECEHNTCGDSCDQCCPGFHQKPWRAGTFLTKTECEACNCHGKAEECYYDENVARRNLSLNIRGKYIGGGVCINCTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLNEVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYP--DCKACNCSGLGSKNED--PCFGPCICKENVEGGD-CSRCKSGFFNLQEDNWKGCDECFCSGVSNRCQSSYWTYGKIQDMSGWYLTDLPGRIRVAPQQDDLDSPQQISISNA--EARQALPHSYYWSAPAPYLGNKLPAVGGQLTFTISYDLEEEEEDTERVLQLMIILEGNDLSISTAQDEVYLHPSEEHTNVLLLKEESFTIHGTHFPV---------RRKEFMTVLANLKRVLLQITYSFGMDAIFRLSSVNLESAVSYPTDGSIAAAVEVCQCPPGYTGSSCESCWPRHRRVNGTIFGGICEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRCNAGGSFSEVCHSQTGQCECRANVQGQRCDKCKAGTFGLQSARGCVPCNCNSFG---SKSFDCEESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTACECS--HLGNNCDPKTGRCICPPNTIGEKCSKCAP-NTWGHSITTGCKACNCSTVGSLDFQCNVNTGQCNCHPKFSGAKCTECSRGHWNYPRCNLCDCFLPGTDATTCDSETKKC------SCSDQTGQCTCKVNVEGIHCDRCRPGKFGLDAKNPLGCSSCYCF 1169 HSP 2 Score: 234.187 bits (596), Expect = 1.979e-60 Identity = 156/424 (36.79%), Postives = 209/424 (49.29%), Query Frame = 2
Query: 2654 CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCDSLGSTSEN------CDKLTGK-CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPNCKKCLC-SPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKS----NELTK--CDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
C+ C+C + ESCD VTGEC C +T G C C GFYG+ ++ C+ C C L S N D+ G CD C + +C+ C G + + C PC C S C++ SG C C G CE C +GYF + NC+ C C + + S C G+C+C +NV + CDKC+ + + CVPCNC GS + C+ E+G C C V + C +C GYF GC C + G C+ + G C+CPPNTIG C+KC+ ++W + T GCK CNC GS CN +G C C F G+KC C+ G++ +P C C C GT + +E K C TGQC CK NV G C CR G F D GC SC+CFG + +C
Sbjct: 754 CEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCAC-PLNIPSNNFSPTCHLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRCNAGGSFSEVCHSQTGQCECRANVQGQRCDKCKAGTFGLQSARGCVPCNCNSFGSKSFDCE-ESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTACECSHLGNNCDPKTGRCICPPNTIGEKCSKCAPNTWGHSITTGCKACNCSTVGSLDFQCNVNTGQCNCHPKFSGAKCTECSRGHWNYPRCNLCDCFLPGTDATTCDSETKKCSCSDQTGQCTCKVNVEGIHCDRCRPGKFGLDAKNPLGCSSCYCFGTTTQC 1175 HSP 3 Score: 221.09 bits (562), Expect = 2.141e-56 Identity = 198/654 (30.28%), Postives = 273/654 (41.74%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCC-PLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTS----FYRCK-----PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKF-TSFQYLTSENSYIDERFNNPTV---------------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGD-----FRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTP--DKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQ--MTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP-KKKISCKRCKCNNNQE---SCDPVTGECRCSYNTMGTECQLCKH 2761
C CN+ K E+G +C C+ G C+ C FN + C C C+ N C K GR CI C NT G C KC W T C C C +G++ C N G C C + G C CS G+ YP C C C + G+ T+ +C C CKV+V C C+ G F LD+ NP GC CYCFG T QCS AK L ++ W T E+ LP + Q+ T++ F +P + +YW P + G + +YG +Y + E + S + P VII+ G + H + +L R I + EWK P + T+E+F+ L + +LIKA Y + + + I++ E P IEKC+CP G++GLSCE+C G+ R+ + G C C C HS+ CD CQNC H+TAG CE CA GYYG K N C++C CP + + C + ++Y C C G +C+RC GY G+P SC+ C+C+ CDPVTG C C G +C CKH
Sbjct: 967 CNCNSFGSKSFDCEESG----QCWCQPGVTGKKCDRCAHGYFNFQE----GGCTACECSHLGNNCD------------PKTGR------CI-CPPNTIGEKCSKCAP---NTWGHSITT--GCKACNCSTVGSLDFQCNVN--------TGQCNCHPKFSGAKCTECSRGHWNYP--RCNLCDCFLPGTDATTCDSETKKCSCSDQTGQCTCKVNVEGIH-CDRCRPGKFGLDAKNPLGCSSCYCFGTTTQCSE--AKGL----IRTW--VTLKAEQTILPLVDEALQHTTTKGIV----FQHPEIVAHMDLMREDLHLEPFYWKLPEQFEGKKLMAYGGKLKYAIYFEAREETGFSTYN-PQVIIRGGTPTHARIIVRHMAAPLIGQLTRHE-IEMTEKEWKYYGDDPRVHRTVTREDFLDILYDIHYILIKATYGNFMRQSRISEISMEVAEQGRGTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLP-NDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKH 1562 HSP 4 Score: 137.117 bits (344), Expect = 5.205e-31 Identity = 167/688 (24.27%), Postives = 291/688 (42.30%), Query Frame = 2
Query: 4289 DSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTA----VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI-KGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLG-ISNELIKNATAYLETIKNIYKEMPNITLA-----ASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLK-ILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISK----SFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVE-------------QQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSY 6244
D ++ +TR+ F+ L ++ + +K Y + + +I I+ +V E Q G +T +EKC C G C+ + G+Y+ S G RT L C CNG+S +CD T CQNC + G+ C+ C G+Y K + C+ C CP S+ NF+ +C E + +C C GY G+ CE C G+ G+P G C+ C+C+ GS + C+ G C C PG G KCD C + E C C CT LL + L + V++ LP+ + N++ ++ LL+ +L Q + + E+ +L TR KV D T D T + + LG EL ++A A E + + + A + K++ + + EL + TQ I D++ + +LK + E N+E + K +++ ++ D + R+ T D +R+ +L A+++ + + +K+ + R+IE+ +K N ++ ++ + D + ++ ++LN + + + KD QA E+++ D AV I K + FN + A F + NIK+ E EK+ K+ K+ ++
Sbjct: 1316 DDPRVHRTVTREDFLDILYDIHYILIKATYGNFMRQS--RISEISMEVAE--QGRGTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRL-----RSQPGG--RTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCV-AEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKHWHAREGWECVFCGDECTGLLLGDLARLEQMVMSINLTGPLPAPYKMLYGLENMTQEL-KHLLSPQRAPERL----------IQLAEGNLNTLVTEMNELLTRATKVTADGEQTGQDAERTNTRAKSLGEFIKELARDAEAVNEKAIKLNETLGTRDEAFERNLEGLQKEIDQMIKELRRKNLETQKEIAEDELVAAEALLKKVKKLFGESRGENEEMEKDLREKLADYK-------NKVDDAWDLLREAT---DKIREA-NRLFAVNQKNMTALEKKKEAVESGKRQIENTLK--EGNDILDEANRLADEINSIIDYVEDIQTKLPPMSEELNDKIDDLSQEIKDRKLAEKVSQA-ESHAAQLNDSSAVLDGILDEAKNISFNAT-----------AAFKA-----YSNIKDYIDEAEKVAKEAKDLAH 1950 HSP 5 Score: 100.908 bits (250), Expect = 5.401e-20 Identity = 87/275 (31.64%), Postives = 117/275 (42.55%), Query Frame = 2
Query: 3086 CLCSPHALSADCD--LNNGKCKCGSNVINRICDKC-ENLHY--WN-----NRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHA 3838
C+C HA + D N +C+C N CD+C H W +T+C CNC + C +NVARR+ S L++ G G VC N C NT G C C++ + N C+ C+C GS + +C K G C CKT F G CDRC GY G+P+C+ C+C+ G+K+ D G C CK+NV G C C+ G F GC CFC GVS +C +
Sbjct: 287 CICYGHARACPLDPATNKSRCECEHNTCGDSCDQCCPGFHQKPWRAGTFLTKTECEACNC---------HGKAEECYYDENVARRNLS--------LNIRG-----KYIGGGVCIN-----CTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLNEVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYPDCKACNCSGLGSKNE-----DPCFGPCICKENVEGGDCSRCKSGFFNLQEDNWKGCDECFCSGVSNRCQS 529 HSP 6 Score: 99.3673 bits (246), Expect = 1.249e-19 Identity = 80/281 (28.47%), Postives = 125/281 (44.48%), Query Frame = 2
Query: 4274 FIILEDSNVINSN---LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY--KNDKNYDQCEPCQCP--SAHYNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSL--NCNEF-GICQ-CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
++ E+S I+ + R FM L N+ + +++ + ++ + + + G AVE C C G+ C++ + + N T G + + C C G+++ CD VTG+C NC + G C C GFY + C+PC CP NF+ C ++ SL L C C +GY+G CE C +G++G P + G C+ C CN S+ +C+ G C C PG G C+ CA Y V+ K CQ C
Sbjct: 653 LLLKEESFTIHGTHFPVRRKEFMTVLANLKRVLLQITYSFGMDAIFRLSSVNLESAVSYPTDGSIAAAVEVCQCPPGYTGSSCESCWPRHRRV-----NGTIFGGI---------CEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCACPLNIPSNNFSPTC-HLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPC 918 HSP 7 Score: 94.3597 bits (233), Expect = 4.449e-18 Identity = 52/143 (36.36%), Postives = 80/143 (55.94%), Query Frame = 2
Query: 4646 CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSC 5068
CDS++G C C G C+LC +G++ + + C+PC+C +A +F++ C S +C+CR G+ C+ C+ G +G +G C C+CN GS+S +C E G C C PGV G KCD+CA Y ++ C +C
Sbjct: 879 CDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRC-NAGGSFSEVC----HSQTGQCECRANVQGQRCDKCKAGTFGLQSARG--CVPCNCNSFGSKSFDCEESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTAC 1014 HSP 8 Score: 69.707 bits (169), Expect = 1.378e-10 Identity = 60/225 (26.67%), Postives = 88/225 (39.11%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY---GNPKKKI------SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLH 3196
C SG+ C+ C+ G+Y P + +C C+CN + CDP T C+ C ++T G C+ C G+YG L C++C C + S++ NF SC + L D +C C G CE+C GY P +C++C C P+ +L CD G C C R CD C++ H
Sbjct: 1382 CPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSN--------------NF----SPSCVAE-GLDDYRCTACP-----------------------RGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKHWH 1564 HSP 9 Score: 63.1586 bits (152), Expect = 1.421e-8 Identity = 61/224 (27.23%), Postives = 96/224 (42.86%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKN---DKNYDQ-CEPCQC-PSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE---FGICQCSPGVLGIKCDQCAPNY 5038
T +C C T G+ C G++++ + ++G+ T + CNC+G ++ C ++V G C NC N G C+ C +GF++ NY + C+PC C P N + C + E C C+ G+ G SC+ C +G+ G P CK+C+C+ GS+ NE FG C C V G C +C +
Sbjct: 301 ATNKSRCECEHNTCGDSCDQCCPGFHQKPW------RAGTFLTKTEC----EACNCHGKAEECYYDENVARRNLSLNIRGKYIGGGVCINCTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLN--EVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYP----DCKACNCSGLGSK----NEDPCFGPCICKENVEGGDCSRCKSGF 504 HSP 10 Score: 58.5362 bits (140), Expect = 3.162e-7 Identity = 56/201 (27.86%), Postives = 84/201 (41.79%), Query Frame = 2
Query: 3236 GRGS-LNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCP--------PCY-NGQ--VCNNE-GICV-CPPNTIGNLCNKCSEDSWDFNETI--GCKKCNCYINGSYSNICNK--ISGI-----CACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCR 3754
GRG+ + P D+ C C + C C G++ L + P PC NG +C+ E IC C +T G+ C +C+ + + + C++C C + S +N G+ AC + G C+RC GY G P +CQ C C G+ CD +TG C C+ G KC C+
Sbjct: 1365 GRGTTMTPPADL-IEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLP---VPCDPVTGFCTCRPGATGRKCDGCK 1561 HSP 11 Score: 58.151 bits (139), Expect = 4.240e-7 Identity = 48/148 (32.43%), Postives = 66/148 (44.59%), Query Frame = 2
Query: 3218 CVPCNC-GRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTG----CKKCP-PCYNGQ-----VCNNEGI-----CVCPPNTIGNLCNKCSED--SWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRC 3604
CVPC C G SL CD ET C+ C + A C +C +GY+G+ V G C++C P + C EG+ CP G C +C+ N C++C C GS C+ ++G C C+ G KCD C
Sbjct: 1417 CVPCQCNGHSSL---CDPETSICQNCQHHTAGDFCERCALGYYGI-VKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGC 1560 HSP 12 Score: 54.299 bits (129), Expect = 5.798e-6 Identity = 55/199 (27.64%), Postives = 77/199 (38.69%), Query Frame = 2
Query: 2819 GSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY-LKDKK-----------CIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPN-CKKCLC----SPHALSADC---DLNNGKC-KCGSNVINRICDKCENLHY---WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKC 3325
G+T L KCDC + C++C G Y L+ + C+PC C C+ + C C + GD CE+C GY+ PN C++C C S + S C L++ +C C + C++C + N C C C GSL CD TG C C R C C
Sbjct: 1367 GTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCN-----GHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGC 1560
BLAST of Laminin subunit alpha-2 vs. Ensembl Human
Match: LAMA2 (laminin subunit alpha 2 [Source:HGNC Symbol;Acc:HGNC:6482]) HSP 1 Score: 715.687 bits (1846), Expect = 0.000e+0 Identity = 433/1176 (36.82%), Postives = 614/1176 (52.21%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLM--THKG--YLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQ--KPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGS--------LIDSPDVIIKCGDFRLFHSVSIEDRR--LQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDE--MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQE-----SCDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYF-------------GLDVTGC----KKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW--DFNETIGCKKCNCY 3514
L NA IT++ATCG GPE++C+LV+ + Q + NP C +C+ + N +RHPI A D N WWQSPS+ G++Y V +T+DL+QV+QI Y+++K +SPRPGNWILERSLD Y PW + A T C YN++ R P PS DDE+ CT++YS+ L +GEI ISLINGR S + P+ L+ F SARYIR RF + TL ADLM HK ++ + RYYYS+++I +GG CIC HA C ++ + ++C C+HNTCG +C+ CCP F+Q+PW+ CE CNC+ K+ EC Y++ VA LS+N G+ GGGVCINC +NT GINCE C GF+RP V PC PC C +G++ E + PG C CK G+ GV+CD C+ GY YP +C C CS GS C PC CK +V D C CK+G+FNL N +GC+ C+C G++N+C S KI D+ GW LT G P+ S Q ++ N+ + R P YYW+AP PYLGN + + G + + +L+ + + +++ D+ I ++ S E L ++ I T + + ++EF++ L + ++L++ Y +I L+ V D +E C CPPG+ G SCESC +RRVN ++ G CE C C+ H+ +CD + GEC NC +T G +C+ C G+YG+ C+ C CP + C S +C C +G C+RC EGY+G P SC+ C+CN+N + SCD ++G C C T G C+LC G++GDA+ K+C+ C C++ GS SE C TG+C+CR N ++CD C +G + + C+PCNC G S+ F SGQC+C V G +C++C GYFN+ C C CS L +CD G+C C N I C KC + W + T C CNC GSL+ +C++ TG C C + C++C G++ G D T C KKC + G C C N G C++C + D +GC C C+
Sbjct: 46 LASNALITTNATCGEKGPEMYCKLVEHVPGQPVRNPQ----CRICNQNSSNPNQRHPITNA-IDGKNTWWQSPSIKNGIEYHYVTITLDLQQVFQIAYVIVKAANSPRPGNWILERSLDDVEYKPWQYHAVTDTECLTLYNIYPRTGP---------PSYAKDDEVICTSFYSKIHPLENGEIHISLINGRPSADDPS-------PE-LLEFTSARYIRLRFQRIRTLNADLMMFAHKDPREIDPIVTRRYYYSVKDISVGGMCICYGHARACP--LDPATNKSRCECEHNTCGDSCDQCCPGFHQKPWRAGTFLTKTECEACNCHGKAEECYYDENVARRNLSLNIRGKYIGGGVCINCTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLNEVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYP--DCKACNCSGLGSKNED--PCFGPCICKENVEGGD-CSRCKSGFFNLQEDNWKGCDECFCSGVSNRCQSSYWTYGKIQDMSGWYLTDLPGRIRVAPQQDDLDSPQQISISNA--EARQALPHSYYWSAPAPYLGNKLPAVGGQLTFTISYDLEEEEEDTERVLQLMIILEGNDLSISTAQDEVYLHPSEEHTNVLLLKEESFTIHGTHFPV---------RRKEFMTVLANLKRVLLQITYSFGMDAIFRLSSVNLESAVSYPTDGSIAAAVEVCQCPPGYTGSSCESCWPRHRRVNGTIFGGICEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRCNAGGSFSEVCHSQTGQCECRANVQGQRCDKCKAGTFGLQSARGCVPCNCNSFG---SKSFDCEESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTACECS--HLGNNCDPKTGRCICPPNTIGEKCSKCAP-NTWGHSITTGCKACNCSTVGSLDFQCNVNTGQCNCHPKFSGAKCTECSRGHWNYPRCNLCDCFLPGTDATTCDSETKKC------SCSDQTGQCTCKVNVEGIHCDRCRPGKFGLDAKNPLGCSSCYCF 1169 HSP 2 Score: 234.187 bits (596), Expect = 2.118e-60 Identity = 156/424 (36.79%), Postives = 209/424 (49.29%), Query Frame = 2
Query: 2654 CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCDSLGSTSEN------CDKLTGK-CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPNCKKCLC-SPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKS----NELTK--CDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
C+ C+C + ESCD VTGEC C +T G C C GFYG+ ++ C+ C C L S N D+ G CD C + +C+ C G + + C PC C S C++ SG C C G CE C +GYF + NC+ C C + + S C G+C+C +NV + CDKC+ + + CVPCNC GS + C+ E+G C C V + C +C GYF GC C + G C+ + G C+CPPNTIG C+KC+ ++W + T GCK CNC GS CN +G C C F G+KC C+ G++ +P C C C GT + +E K C TGQC CK NV G C CR G F D GC SC+CFG + +C
Sbjct: 754 CEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCAC-PLNIPSNNFSPTCHLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRCNAGGSFSEVCHSQTGQCECRANVQGQRCDKCKAGTFGLQSARGCVPCNCNSFGSKSFDCE-ESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTACECSHLGNNCDPKTGRCICPPNTIGEKCSKCAPNTWGHSITTGCKACNCSTVGSLDFQCNVNTGQCNCHPKFSGAKCTECSRGHWNYPRCNLCDCFLPGTDATTCDSETKKCSCSDQTGQCTCKVNVEGIHCDRCRPGKFGLDAKNPLGCSSCYCFGTTTQC 1175 HSP 3 Score: 221.09 bits (562), Expect = 2.125e-56 Identity = 198/654 (30.28%), Postives = 273/654 (41.74%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCC-PLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTS----FYRCK-----PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKF-TSFQYLTSENSYIDERFNNPTV---------------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGD-----FRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTP--DKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQ--MTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP-KKKISCKRCKCNNNQE---SCDPVTGECRCSYNTMGTECQLCKH 2761
C CN+ K E+G +C C+ G C+ C FN + C C C+ N C K GR CI C NT G C KC W T C C C +G++ C N G C C + G C CS G+ YP C C C + G+ T+ +C C CKV+V C C+ G F LD+ NP GC CYCFG T QCS AK L ++ W T E+ LP + Q+ T++ F +P + +YW P + G + +YG +Y + E + S + P VII+ G + H + +L R I + EWK P + T+E+F+ L + +LIKA Y + + + I++ E P IEKC+CP G++GLSCE+C G+ R+ + G C C C HS+ CD CQNC H+TAG CE CA GYYG K N C++C CP + + C + ++Y C C G +C+RC GY G+P SC+ C+C+ CDPVTG C C G +C CKH
Sbjct: 967 CNCNSFGSKSFDCEESG----QCWCQPGVTGKKCDRCAHGYFNFQE----GGCTACECSHLGNNCD------------PKTGR------CI-CPPNTIGEKCSKCAP---NTWGHSITT--GCKACNCSTVGSLDFQCNVN--------TGQCNCHPKFSGAKCTECSRGHWNYP--RCNLCDCFLPGTDATTCDSETKKCSCSDQTGQCTCKVNVEGIH-CDRCRPGKFGLDAKNPLGCSSCYCFGTTTQCSE--AKGL----IRTW--VTLKAEQTILPLVDEALQHTTTKGIV----FQHPEIVAHMDLMREDLHLEPFYWKLPEQFEGKKLMAYGGKLKYAIYFEAREETGFSTYN-PQVIIRGGTPTHARIIVRHMAAPLIGQLTRHE-IEMTEKEWKYYGDDPRVHRTVTREDFLDILYDIHYILIKATYGNFMRQSRISEISMEVAEQGRGTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLP-NDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKH 1562 HSP 4 Score: 137.117 bits (344), Expect = 5.662e-31 Identity = 167/688 (24.27%), Postives = 291/688 (42.30%), Query Frame = 2
Query: 4289 DSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTA----VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI-KGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLG-ISNELIKNATAYLETIKNIYKEMPNITLA-----ASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLK-ILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISK----SFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVE-------------QQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSY 6244
D ++ +TR+ F+ L ++ + +K Y + + +I I+ +V E Q G +T +EKC C G C+ + G+Y+ S G RT L C CNG+S +CD T CQNC + G+ C+ C G+Y K + C+ C CP S+ NF+ +C E + +C C GY G+ CE C G+ G+P G C+ C+C+ GS + C+ G C C PG G KCD C + E C C CT LL + L + V++ LP+ + N++ ++ LL+ +L Q + + E+ +L TR KV D T D T + + LG EL ++A A E + + + A + K++ + + EL + TQ I D++ + +LK + E N+E + K +++ ++ D + R+ T D +R+ +L A+++ + + +K+ + R+IE+ +K N ++ ++ + D + ++ ++LN + + + KD QA E+++ D AV I K + FN + A F + NIK+ E EK+ K+ K+ ++
Sbjct: 1316 DDPRVHRTVTREDFLDILYDIHYILIKATYGNFMRQS--RISEISMEVAE--QGRGTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRL-----RSQPGG--RTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCV-AEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKHWHAREGWECVFCGDECTGLLLGDLARLEQMVMSINLTGPLPAPYKMLYGLENMTQEL-KHLLSPQRAPERL----------IQLAEGNLNTLVTEMNELLTRATKVTADGEQTGQDAERTNTRAKSLGEFIKELARDAEAVNEKAIKLNETLGTRDEAFERNLEGLQKEIDQMIKELRRKNLETQKEIAEDELVAAEALLKKVKKLFGESRGENEEMEKDLREKLADYK-------NKVDDAWDLLREAT---DKIREA-NRLFAVNQKNMTALEKKKEAVESGKRQIENTLK--EGNDILDEANRLADEINSIIDYVEDIQTKLPPMSEELNDKIDDLSQEIKDRKLAEKVSQA-ESHAAQLNDSSAVLDGILDEAKNISFNAT-----------AAFKA-----YSNIKDYIDEAEKVAKEAKDLAH 1950 HSP 5 Score: 100.908 bits (250), Expect = 5.313e-20 Identity = 87/275 (31.64%), Postives = 117/275 (42.55%), Query Frame = 2
Query: 3086 CLCSPHALSADCD--LNNGKCKCGSNVINRICDKC-ENLHY--WN-----NRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHA 3838
C+C HA + D N +C+C N CD+C H W +T+C CNC + C +NVARR+ S L++ G G VC N C NT G C C++ + N C+ C+C GS + +C K G C CKT F G CDRC GY G+P+C+ C+C+ G+K+ D G C CK+NV G C C+ G F GC CFC GVS +C +
Sbjct: 287 CICYGHARACPLDPATNKSRCECEHNTCGDSCDQCCPGFHQKPWRAGTFLTKTECEACNC---------HGKAEECYYDENVARRNLS--------LNIRG-----KYIGGGVCIN-----CTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLNEVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYPDCKACNCSGLGSKNE-----DPCFGPCICKENVEGGDCSRCKSGFFNLQEDNWKGCDECFCSGVSNRCQS 529 HSP 6 Score: 99.3673 bits (246), Expect = 1.325e-19 Identity = 80/281 (28.47%), Postives = 125/281 (44.48%), Query Frame = 2
Query: 4274 FIILEDSNVINSN---LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY--KNDKNYDQCEPCQCP--SAHYNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSL--NCNEF-GICQ-CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
++ E+S I+ + R FM L N+ + +++ + ++ + + + G AVE C C G+ C++ + + N T G + + C C G+++ CD VTG+C NC + G C C GFY + C+PC CP NF+ C ++ SL L C C +GY+G CE C +G++G P + G C+ C CN S+ +C+ G C C PG G C+ CA Y V+ K CQ C
Sbjct: 653 LLLKEESFTIHGTHFPVRRKEFMTVLANLKRVLLQITYSFGMDAIFRLSSVNLESAVSYPTDGSIAAAVEVCQCPPGYTGSSCESCWPRHRRV-----NGTIFGGI---------CEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCACPLNIPSNNFSPTC-HLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPC 918 HSP 7 Score: 94.3597 bits (233), Expect = 4.525e-18 Identity = 52/143 (36.36%), Postives = 80/143 (55.94%), Query Frame = 2
Query: 4646 CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSC 5068
CDS++G C C G C+LC +G++ + + C+PC+C +A +F++ C S +C+CR G+ C+ C+ G +G +G C C+CN GS+S +C E G C C PGV G KCD+CA Y ++ C +C
Sbjct: 879 CDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRC-NAGGSFSEVC----HSQTGQCECRANVQGQRCDKCKAGTFGLQSARG--CVPCNCNSFGSKSFDCEESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTAC 1014 HSP 8 Score: 69.707 bits (169), Expect = 1.449e-10 Identity = 60/225 (26.67%), Postives = 88/225 (39.11%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY---GNPKKKI------SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLH 3196
C SG+ C+ C+ G+Y P + +C C+CN + CDP T C+ C ++T G C+ C G+YG L C++C C + S++ NF SC + L D +C C G CE+C GY P +C++C C P+ +L CD G C C R CD C++ H
Sbjct: 1382 CPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSN--------------NF----SPSCVAE-GLDDYRCTACP-----------------------RGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKHWH 1564 HSP 9 Score: 63.1586 bits (152), Expect = 1.410e-8 Identity = 61/224 (27.23%), Postives = 96/224 (42.86%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKN---DKNYDQ-CEPCQC-PSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE---FGICQCSPGVLGIKCDQCAPNY 5038
T +C C T G+ C G++++ + ++G+ T + CNC+G ++ C ++V G C NC N G C+ C +GF++ NY + C+PC C P N + C + E C C+ G+ G SC+ C +G+ G P CK+C+C+ GS+ NE FG C C V G C +C +
Sbjct: 301 ATNKSRCECEHNTCGDSCDQCCPGFHQKPW------RAGTFLTKTEC----EACNCHGKAEECYYDENVARRNLSLNIRGKYIGGGVCINCTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLN--EVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYP----DCKACNCSGLGSK----NEDPCFGPCICKENVEGGDCSRCKSGF 504 HSP 10 Score: 58.5362 bits (140), Expect = 3.058e-7 Identity = 56/201 (27.86%), Postives = 84/201 (41.79%), Query Frame = 2
Query: 3236 GRGS-LNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCP--------PCY-NGQ--VCNNE-GICV-CPPNTIGNLCNKCSEDSWDFNETI--GCKKCNCYINGSYSNICNK--ISGI-----CACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCR 3754
GRG+ + P D+ C C + C C G++ L + P PC NG +C+ E IC C +T G+ C +C+ + + + C++C C + S +N G+ AC + G C+RC GY G P +CQ C C G+ CD +TG C C+ G KC C+
Sbjct: 1365 GRGTTMTPPADL-IEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLP---VPCDPVTGFCTCRPGATGRKCDGCK 1561 HSP 11 Score: 58.151 bits (139), Expect = 4.205e-7 Identity = 48/148 (32.43%), Postives = 66/148 (44.59%), Query Frame = 2
Query: 3218 CVPCNC-GRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTG----CKKCP-PCYNGQ-----VCNNEGI-----CVCPPNTIGNLCNKCSED--SWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRC 3604
CVPC C G SL CD ET C+ C + A C +C +GY+G+ V G C++C P + C EG+ CP G C +C+ N C++C C GS C+ ++G C C+ G KCD C
Sbjct: 1417 CVPCQCNGHSSL---CDPETSICQNCQHHTAGDFCERCALGYYGI-VKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGC 1560 HSP 12 Score: 54.299 bits (129), Expect = 5.703e-6 Identity = 55/199 (27.64%), Postives = 77/199 (38.69%), Query Frame = 2
Query: 2819 GSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY-LKDKK-----------CIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPN-CKKCLC----SPHALSADC---DLNNGKC-KCGSNVINRICDKCENLHY---WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKC 3325
G+T L KCDC + C++C G Y L+ + C+PC C C+ + C C + GD CE+C GY+ PN C++C C S + S C L++ +C C + C++C + N C C C GSL CD TG C C R C C
Sbjct: 1367 GTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCN-----GHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGC 1560
BLAST of Laminin subunit alpha-2 vs. Ensembl Human
Match: LAMA2 (laminin subunit alpha 2 [Source:HGNC Symbol;Acc:HGNC:6482]) HSP 1 Score: 715.301 bits (1845), Expect = 0.000e+0 Identity = 433/1176 (36.82%), Postives = 614/1176 (52.21%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLM--THKG--YLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQ--KPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGS--------LIDSPDVIIKCGDFRLFHSVSIEDRR--LQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDE--MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQE-----SCDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYF-------------GLDVTGC----KKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW--DFNETIGCKKCNCY 3514
L NA IT++ATCG GPE++C+LV+ + Q + NP C +C+ + N +RHPI A D N WWQSPS+ G++Y V +T+DL+QV+QI Y+++K +SPRPGNWILERSLD Y PW + A T C YN++ R P PS DDE+ CT++YS+ L +GEI ISLINGR S + P+ L+ F SARYIR RF + TL ADLM HK ++ + RYYYS+++I +GG CIC HA C ++ + ++C C+HNTCG +C+ CCP F+Q+PW+ CE CNC+ K+ EC Y++ VA LS+N G+ GGGVCINC +NT GINCE C GF+RP V PC PC C +G++ E + PG C CK G+ GV+CD C+ GY YP +C C CS GS C PC CK +V D C CK+G+FNL N +GC+ C+C G++N+C S KI D+ GW LT G P+ S Q ++ N+ + R P YYW+AP PYLGN + + G + + +L+ + + +++ D+ I ++ S E L ++ I T + + ++EF++ L + ++L++ Y +I L+ V D +E C CPPG+ G SCESC +RRVN ++ G CE C C+ H+ +CD + GEC NC +T G +C+ C G+YG+ C+ C CP + C S +C C +G C+RC EGY+G P SC+ C+CN+N + SCD ++G C C T G C+LC G++GDA+ K+C+ C C++ GS SE C TG+C+CR N ++CD C +G + + C+PCNC G S+ F SGQC+C V G +C++C GYFN+ C C CS L +CD G+C C N I C KC + W + T C CNC GSL+ +C++ TG C C + C++C G++ G D T C KKC + G C C N G C++C + D +GC C C+
Sbjct: 46 LASNALITTNATCGEKGPEMYCKLVEHVPGQPVRNPQ----CRICNQNSSNPNQRHPITNA-IDGKNTWWQSPSIKNGIEYHYVTITLDLQQVFQIAYVIVKAANSPRPGNWILERSLDDVEYKPWQYHAVTDTECLTLYNIYPRTGP---------PSYAKDDEVICTSFYSKIHPLENGEIHISLINGRPSADDPS-------PE-LLEFTSARYIRLRFQRIRTLNADLMMFAHKDPREIDPIVTRRYYYSVKDISVGGMCICYGHARACP--LDPATNKSRCECEHNTCGDSCDQCCPGFHQKPWRAGTFLTKTECEACNCHGKAEECYYDENVARRNLSLNIRGKYIGGGVCINCTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLNEVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYP--DCKACNCSGLGSKNED--PCFGPCICKENVEGGD-CSRCKSGFFNLQEDNWKGCDECFCSGVSNRCQSSYWTYGKIQDMSGWYLTDLPGRIRVAPQQDDLDSPQQISISNA--EARQALPHSYYWSAPAPYLGNKLPAVGGQLTFTISYDLEEEEEDTERVLQLMIILEGNDLSISTAQDEVYLHPSEEHTNVLLLKEESFTIHGTHFPV---------RRKEFMTVLANLKRVLLQITYSFGMDAIFRLSSVNLESAVSYPTDGSIAAAVEVCQCPPGYTGSSCESCWPRHRRVNGTIFGGICEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRCNAGGSFSEVCHSQTGQCECRANVQGQRCDKCKAGTFGLQSARGCVPCNCNSFG---SKSFDCEESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTACECS--HLGNNCDPKTGRCICPPNTIGEKCSKCAP-NTWGHSITTGCKACNCSTVGSLDFQCNVNTGQCNCHPKFSGAKCTECSRGHWNYPRCNLCDCFLPGTDATTCDSETKKC------SCSDQTGQCTCKVNVEGIHCDRCRPGKFGLDAKNPLGCSSCYCF 1169 HSP 2 Score: 234.187 bits (596), Expect = 2.190e-60 Identity = 156/424 (36.79%), Postives = 209/424 (49.29%), Query Frame = 2
Query: 2654 CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCDSLGSTSEN------CDKLTGK-CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPNCKKCLC-SPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKS----NELTK--CDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
C+ C+C + ESCD VTGEC C +T G C C GFYG+ ++ C+ C C L S N D+ G CD C + +C+ C G + + C PC C S C++ SG C C G CE C +GYF + NC+ C C + + S C G+C+C +NV + CDKC+ + + CVPCNC GS + C+ E+G C C V + C +C GYF GC C + G C+ + G C+CPPNTIG C+KC+ ++W + T GCK CNC GS CN +G C C F G+KC C+ G++ +P C C C GT + +E K C TGQC CK NV G C CR G F D GC SC+CFG + +C
Sbjct: 754 CEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCAC-PLNIPSNNFSPTCHLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRCNAGGSFSEVCHSQTGQCECRANVQGQRCDKCKAGTFGLQSARGCVPCNCNSFGSKSFDCE-ESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTACECSHLGNNCDPKTGRCICPPNTIGEKCSKCAPNTWGHSITTGCKACNCSTVGSLDFQCNVNTGQCNCHPKFSGAKCTECSRGHWNYPRCNLCDCFLPGTDATTCDSETKKCSCSDQTGQCTCKVNVEGIHCDRCRPGKFGLDAKNPLGCSSCYCFGTTTQC 1175 HSP 3 Score: 220.705 bits (561), Expect = 2.330e-56 Identity = 198/654 (30.28%), Postives = 273/654 (41.74%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCC-PLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTS----FYRCK-----PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKF-TSFQYLTSENSYIDERFNNPTV---------------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGD-----FRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTP--DKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQ--MTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP-KKKISCKRCKCNNNQE---SCDPVTGECRCSYNTMGTECQLCKH 2761
C CN+ K E+G +C C+ G C+ C FN + C C C+ N C K GR CI C NT G C KC W T C C C +G++ C N G C C + G C CS G+ YP C C C + G+ T+ +C C CKV+V C C+ G F LD+ NP GC CYCFG T QCS AK L ++ W T E+ LP + Q+ T++ F +P + +YW P + G + +YG +Y + E + S + P VII+ G + H + +L R I + EWK P + T+E+F+ L + +LIKA Y + + + I++ E P IEKC+CP G++GLSCE+C G+ R+ + G C C C HS+ CD CQNC H+TAG CE CA GYYG K N C++C CP + + C + ++Y C C G +C+RC GY G+P SC+ C+C+ CDPVTG C C G +C CKH
Sbjct: 967 CNCNSFGSKSFDCEESG----QCWCQPGVTGKKCDRCAHGYFNFQE----GGCTACECSHLGNNCD------------PKTGR------CI-CPPNTIGEKCSKCAP---NTWGHSITT--GCKACNCSTVGSLDFQCNVN--------TGQCNCHPKFSGAKCTECSRGHWNYP--RCNLCDCFLPGTDATTCDSETKKCSCSDQTGQCTCKVNVEGIH-CDRCRPGKFGLDAKNPLGCSSCYCFGTTTQCSE--AKGL----IRTW--VTLKAEQTILPLVDEALQHTTTKGIV----FQHPEIVAHMDLMREDLHLEPFYWKLPEQFEGKKLMAYGGKLKYAIYFEAREETGFSTYN-PQVIIRGGTPTHARIIVRHMAAPLIGQLTRHE-IEMTEKEWKYYGDDPRVHRTVTREDFLDILYDIHYILIKATYGNFMRQSRISEISMEVAEQGRGTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLP-NDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKH 1562 HSP 4 Score: 136.732 bits (343), Expect = 6.209e-31 Identity = 167/688 (24.27%), Postives = 291/688 (42.30%), Query Frame = 2
Query: 4289 DSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTA----VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI-KGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLG-ISNELIKNATAYLETIKNIYKEMPNITLA-----ASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLK-ILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISK----SFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVE-------------QQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSY 6244
D ++ +TR+ F+ L ++ + +K Y + + +I I+ +V E Q G +T +EKC C G C+ + G+Y+ S G RT L C CNG+S +CD T CQNC + G+ C+ C G+Y K + C+ C CP S+ NF+ +C E + +C C GY G+ CE C G+ G+P G C+ C+C+ GS + C+ G C C PG G KCD C + E C C CT LL + L + V++ LP+ + N++ ++ LL+ +L Q + + E+ +L TR KV D T D T + + LG EL ++A A E + + + A + K++ + + EL + TQ I D++ + +LK + E N+E + K +++ ++ D + R+ T D +R+ +L A+++ + + +K+ + R+IE+ +K N ++ ++ + D + ++ ++LN + + + KD QA E+++ D AV I K + FN + A F + NIK+ E EK+ K+ K+ ++
Sbjct: 1316 DDPRVHRTVTREDFLDILYDIHYILIKATYGNFMRQS--RISEISMEVAE--QGRGTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRL-----RSQPGG--RTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCV-AEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKHWHAREGWECVFCGDECTGLLLGDLARLEQMVMSINLTGPLPAPYKMLYGLENMTQEL-KHLLSPQRAPERL----------IQLAEGNLNTLVTEMNELLTRATKVTADGEQTGQDAERTNTRAKSLGEFIKELARDAEAVNEKAIKLNETLGTRDEAFERNLEGLQKEIDQMIKELRRKNLETQKEIAEDELVAAEALLKKVKKLFGESRGENEEMEKDLREKLADYK-------NKVDDAWDLLREAT---DKIREA-NRLFAVNQKNMTALEKKKEAVESGKRQIENTLK--EGNDILDEANRLADEINSIIDYVEDIQTKLPPMSEELNDKIDDLSQEIKDRKLAEKVSQA-ESHAAQLNDSSAVLDGILDEAKNISFNAT-----------AAFKA-----YSNIKDYIDEAEKVAKEAKDLAH 1950 HSP 5 Score: 100.523 bits (249), Expect = 5.681e-20 Identity = 87/275 (31.64%), Postives = 117/275 (42.55%), Query Frame = 2
Query: 3086 CLCSPHALSADCD--LNNGKCKCGSNVINRICDKC-ENLHY--WN-----NRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHA 3838
C+C HA + D N +C+C N CD+C H W +T+C CNC + C +NVARR+ S L++ G G VC N C NT G C C++ + N C+ C+C GS + +C K G C CKT F G CDRC GY G+P+C+ C+C+ G+K+ D G C CK+NV G C C+ G F GC CFC GVS +C +
Sbjct: 287 CICYGHARACPLDPATNKSRCECEHNTCGDSCDQCCPGFHQKPWRAGTFLTKTECEACNC---------HGKAEECYYDENVARRNLS--------LNIRG-----KYIGGGVCIN-----CTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLNEVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYPDCKACNCSGLGSKNE-----DPCFGPCICKENVEGGDCSRCKSGFFNLQEDNWKGCDECFCSGVSNRCQS 529 HSP 6 Score: 99.3673 bits (246), Expect = 1.325e-19 Identity = 80/281 (28.47%), Postives = 125/281 (44.48%), Query Frame = 2
Query: 4274 FIILEDSNVINSN---LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY--KNDKNYDQCEPCQCP--SAHYNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSL--NCNEF-GICQ-CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
++ E+S I+ + R FM L N+ + +++ + ++ + + + G AVE C C G+ C++ + + N T G + + C C G+++ CD VTG+C NC + G C C GFY + C+PC CP NF+ C ++ SL L C C +GY+G CE C +G++G P + G C+ C CN S+ +C+ G C C PG G C+ CA Y V+ K CQ C
Sbjct: 653 LLLKEESFTIHGTHFPVRRKEFMTVLANLKRVLLQITYSFGMDAIFRLSSVNLESAVSYPTDGSIAAAVEVCQCPPGYTGSSCESCWPRHRRV-----NGTIFGGI---------CEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCACPLNIPSNNFSPTC-HLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPC 918 HSP 7 Score: 94.3597 bits (233), Expect = 4.601e-18 Identity = 52/143 (36.36%), Postives = 80/143 (55.94%), Query Frame = 2
Query: 4646 CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSC 5068
CDS++G C C G C+LC +G++ + + C+PC+C +A +F++ C S +C+CR G+ C+ C+ G +G +G C C+CN GS+S +C E G C C PGV G KCD+CA Y ++ C +C
Sbjct: 879 CDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRC-NAGGSFSEVC----HSQTGQCECRANVQGQRCDKCKAGTFGLQSARG--CVPCNCNSFGSKSFDCEESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTAC 1014 HSP 8 Score: 69.707 bits (169), Expect = 1.449e-10 Identity = 60/225 (26.67%), Postives = 88/225 (39.11%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY---GNPKKKI------SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLH 3196
C SG+ C+ C+ G+Y P + +C C+CN + CDP T C+ C ++T G C+ C G+YG L C++C C + S++ NF SC + L D +C C G CE+C GY P +C++C C P+ +L CD G C C R CD C++ H
Sbjct: 1382 CPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSN--------------NF----SPSCVAE-GLDDYRCTACP-----------------------RGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKHWH 1564 HSP 9 Score: 62.7734 bits (151), Expect = 1.533e-8 Identity = 61/224 (27.23%), Postives = 96/224 (42.86%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKN---DKNYDQ-CEPCQC-PSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE---FGICQCSPGVLGIKCDQCAPNY 5038
T +C C T G+ C G++++ + ++G+ T + CNC+G ++ C ++V G C NC N G C+ C +GF++ NY + C+PC C P N + C + E C C+ G+ G SC+ C +G+ G P CK+C+C+ GS+ NE FG C C V G C +C +
Sbjct: 301 ATNKSRCECEHNTCGDSCDQCCPGFHQKPW------RAGTFLTKTEC----EACNCHGKAEECYYDENVARRNLSLNIRGKYIGGGVCINCTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLN--EVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYP----DCKACNCSGLGSK----NEDPCFGPCICKENVEGGDCSRCKSGF 504 HSP 10 Score: 58.5362 bits (140), Expect = 3.109e-7 Identity = 56/201 (27.86%), Postives = 84/201 (41.79%), Query Frame = 2
Query: 3236 GRGS-LNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCP--------PCY-NGQ--VCNNE-GICV-CPPNTIGNLCNKCSEDSWDFNETI--GCKKCNCYINGSYSNICNK--ISGI-----CACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCR 3754
GRG+ + P D+ C C + C C G++ L + P PC NG +C+ E IC C +T G+ C +C+ + + + C++C C + S +N G+ AC + G C+RC GY G P +CQ C C G+ CD +TG C C+ G KC C+
Sbjct: 1365 GRGTTMTPPADL-IEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLP---VPCDPVTGFCTCRPGATGRKCDGCK 1561 HSP 11 Score: 58.151 bits (139), Expect = 4.276e-7 Identity = 48/148 (32.43%), Postives = 66/148 (44.59%), Query Frame = 2
Query: 3218 CVPCNC-GRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTG----CKKCP-PCYNGQ-----VCNNEGI-----CVCPPNTIGNLCNKCSED--SWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRC 3604
CVPC C G SL CD ET C+ C + A C +C +GY+G+ V G C++C P + C EG+ CP G C +C+ N C++C C GS C+ ++G C C+ G KCD C
Sbjct: 1417 CVPCQCNGHSSL---CDPETSICQNCQHHTAGDFCERCALGYYGI-VKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGC 1560 HSP 12 Score: 54.299 bits (129), Expect = 5.897e-6 Identity = 55/199 (27.64%), Postives = 77/199 (38.69%), Query Frame = 2
Query: 2819 GSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY-LKDKK-----------CIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPN-CKKCLC----SPHALSADC---DLNNGKC-KCGSNVINRICDKCENLHY---WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKC 3325
G+T L KCDC + C++C G Y L+ + C+PC C C+ + C C + GD CE+C GY+ PN C++C C S + S C L++ +C C + C++C + N C C C GSL CD TG C C R C C
Sbjct: 1367 GTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCN-----GHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGC 1560
BLAST of Laminin subunit alpha-2 vs. Ensembl Human
Match: LAMC1 (laminin subunit gamma 1 [Source:HGNC Symbol;Acc:HGNC:6492]) HSP 1 Score: 430.254 bits (1105), Expect = 1.877e-122 Identity = 313/1052 (29.75%), Postives = 492/1052 (46.77%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAK---SDDDNLWWQSPSMAQGLKY-QNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSL--DGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNL-KIS-----DLKGWKLTTFTGEKIALPKFTSFQYLTS-ENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR-----LFHSVSIEDRRLQRQVFIYIKPTE--WKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRR-VNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS----CKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS----CKKCDCDSLGSTSE--NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--PNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWN 3205
N + ++ TCGT PE +C + + C +CD +H F + D WWQS +M G++Y ++N+T+ L + + I Y+ LK S RP ++ + + DG PWI + SC N + + T + +L CT +S L G + S + GR S N + + L +++A IR + T ++ + + + YYY++ + +GG+C CN HA +C ++N CNCKHNT G +CE C P FN RPW+ C C+CN +S EC ++ ++ R G GG C NCQ+NT+G +CE+C + F+R N A C+ C C +G++ + C S G C CK G G CD C G+ C PC C GS+ C CK +V + C+ CK G+FNL+S+NP GC PC+CFG ++ C++ ++ IS D GW+ G + +L + Q + +SY Y+ AP +LG + SYG + +++ + T + + D++++ R + S + VF + T+ W+ A T EF L + + I+ YS + + L ++TL+ + +E C CP G+ G CE C SGYRR N+ C CAC HS TCD G C NC NTAG HCE C+ GYYGD+ + + C+ CPCP + ++ VC C T+G C+ C +GY+G+P + C+ C+C++N + +C+ +TGEC +C YNT G C CK GF+G+ L + CK C+C+ G+ + +C+ +TG+C+C + + C +C G Y + C C+C G + + C+ +GQC C + G CE+C +F + CK C C P +LS C ++G+C+C + CD+CE +++N
Sbjct: 60 NVTVVATNTCGTP-PEEYCVQTGVTGVTKS--------CHLCDAGQPHLQHGAAFLTDYNNQADTTWWQSQTMLAGVQYPSSINLTLHLGKAFDITYVRLKFHTS-RPESFAIYKRTREDG----PWIPYQYYSGSCENTYSKANRGFIRTGGDEQQAL------CTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPV------LQEWVTATDIRVTLNRLNTFGDEV-----FNDPKVLKSYYYAISDFAVGGRCKCNGHASEC---MKNEFDKLVCNCKHNTYGVDCEKCLPFFNDRPWRRATAESASECLPCDCNGRSQECYFDP----------ELYRSTGHGGHCTNCQDNTDGAHCERCRENFFRLGNNEA-----CSSCHCSPVGSLSTQCDSYGR---------CSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDPSGSIDECNIETGR-CVCKDNVEGFN-CERCKPGFFNLESSNPRGCTPCFCFGHSSVCTNAVGYSVYSISSTFQIDEDGWRAEQRDGSEASLEWSSERQDIAVISDSYFPR--------YFIAPAKFLGKQVLSYGQNLSFSFRVDRRDT----RLSAEDLVLEGAGLRVSVPLIAQGNSYPSETTVKYVFRLHEATDYPWR-------PALTPFEFQKLLNNLTSIKIRGT-YSERSAGYLDDVTLASARPGPGVPAT-WVESCTCPVGYGGQFCEMCLSGYRRETPNLGPYSPCVLCACNGHSETCDPETGVC-NCRDNTAGPHCEKCSDGYYGDSTAGTSSDCQPCPCP----GGSSCAVVPKTKEVVCTNCPTGTTGKRCELCDDGYFGDPLGRNGPVRLCRLCQCSDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKDGFFGNPLAPNPADKCKACNCNLYGTMKQQSSCNPVTGQCECLPHVTGQDCGACDPGFYNLQSGQGCERCDCHALGSTNGQ--CDIRTGQCECQPGITGQHCERCEVNHFGFGPEGCKPCDCHPEGSLSLQCK-DDGRCECREGFVGNRCDQCEENYFYN 1022 HSP 2 Score: 160.229 bits (404), Expect = 3.827e-38 Identity = 170/626 (27.16%), Postives = 256/626 (40.89%), Query Frame = 2
Query: 3281 CKCAQNVARRDCSKC-----DVGYFGLDVTGCKKCPPCY-NG--QVC----------NNEGICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRR-FGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQ---YQNDILDKFII-LEDSN--VINSNLTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI-KGIP---CKSCDC--NQQGSQSLNCNEF-GIC-QCSPGVLGIKCDQCAPNY 5038
C C N DC KC D + +C PC NG Q C + G C C NT G C +C E+ + C C+C GS S C+ G C+CK G KCDRC G++ C+PCSC +G+ + +C+ TG+C CK NV G C+ C+ G F + + GC CFCFG S C + + + SI DE +D S L + R+ + P Y F + + +++ + VD D + E ++ +E G+R V +I+Q Y ++ K++ L ++ LT F L N+TS+K++ Y+++ L + + + + G T VE C C G C+ +SGY +E NL + +L C CNG+S+ CD TG C NC N G C+ C +G+Y + C+PC CP +C + + E+ C C G +GK CE C G++G+P+ + P C+ C C N + NCN G C +C G CD+C +
Sbjct: 305 CNCKHNTYGVDCEKCLPFFNDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGHGGHCTNCQDNTDGAHCERCRENFFRLGNNEACSSCHCSPVGSLSTQCDSY-GRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDPSGS----IDECNIETGRCVCKDNVEGFNCERCKPGFFNLESSNPRGCTPCFCFGHSSVCTNAVGYSVY--SISSTFQIDEDGWRAEQRDGSEASLEWSSERQDIAVISDSYFPRYFIAPAKFLGKQVLSYGQNLSFSFRVDRRDTRLSAE-----DLVLEGA----GLRVSVP----------------------LIAQGNSYPSETTVKYVFRLHEATDYPWRPALTPFEFQKLLNNLTSIKIRGTYSERSAGYLDDVTLASAR-----PGPGVPATWVESCTCPVGYGGQFCEMCLSGYRRETPNLGPYSPC--------VL-----CACNGHSETCDPETGVC-NCRDNTAGPHCEKCSDGYYGDSTAGTSSDCQPCPCPG-----GSSCAVVPKTKEVVCTNCPTGTTGKRCELCDDGYFGDPLGRNGPVRLCRLCQCSDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKDGF 868 HSP 3 Score: 135.961 bits (341), Expect = 1.025e-30 Identity = 115/351 (32.76%), Postives = 154/351 (43.87%), Query Frame = 2
Query: 2711 CRCSYNTMGTECQLCKHGFYGDALT----KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKD-----KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYPN--------CKKCLCS----PHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWN----NRTD-CVPCNC---GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV-TGCKKCPPCY-----NGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY---GFPNCQPC 3643
C C G C++C G+ + C C C+ SE CD TG C+CR N C+ C+ G Y C PC C G S T C C G RCE C +GYF P C+ C CS P+A+ +C+ G+C KC N CD+C++ + N N D C CNC G C+ TG C+C +V +DC CD G++ L GC++C C+ NGQ G C C P G C +C + + F GCK C+C+ GS S C K G C C+ F G++CD+C E Y+ +P CQ C
Sbjct: 690 CTCPVGYGGQFCEMCLSGYRRETPNLGPYSPCVLCACNG---HSETCDPETGVCNCRDNTAGPHCEKCSDGYYGDSTAGTSSDCQPCPC--PGGSSCAVVPKTKEVVCTNCPTGTTGKRCELCDDGYFGDPLGRNGPVRLCRLCQCSDNIDPNAV-GNCNRLTGECLKCIYNTAGFYCDRCKDGFFGNPLAPNPADKCKACNCNLYGTMKQQSSCNPVTGQCECLPHVTGQDCGACDPGFYNLQSGQGCERCD-CHALGSTNGQCDIRTGQCECQPGITGQHCERCEVNHFGFGPE-GCKPCDCHPEGSLSLQC-KDDGRCECREGFVGNRCDQCEENYFYNRSWPGCQEC 1031 HSP 4 Score: 132.109 bits (331), Expect = 1.456e-29 Identity = 98/341 (28.74%), Postives = 142/341 (41.64%), Query Frame = 2
Query: 2594 GVHCDRCIEGYY---GNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDA---LTKSCKKCDCDS-------------------LGSTSENCD--------------------------------------KLTGKC-DCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN---CKKCLCSPHALSA---DCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYF-GLDVTGCKKCPPCY 3382
G C+ C+ GY N C C CN + E+CDP TG C C NT G C+ C G+YGD+ + C+ C C G+T + C+ +LTG+C C N CD C G + KC CNC + G + + CN +GQC CL +V G C C G++N + C++C C HAL + CD+ G+C+C + + C++CE H+ C PC+C GSL+ +C + G C+C + C +C+ YF GC++CP CY
Sbjct: 698 GQFCEMCLSGYRRETPNLGPYSPCVLCACNGHSETCDPETGVCNCRDNTAGPHCEKCSDGYYGDSTAGTSSDCQPCPCPGGSSCAVVPKTKEVVCTNCPTGTTGKRCELCDDGYFGDPLGRNGPVRLCRLCQCSDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKDGFFGNPLAPNPADKCKACNCNLYGTMKQQSSCNPVTGQCECLPHVTGQDCGACDPGFYNLQSGQGCERCDC--HALGSTNGQCDIRTGQCECQPGITGQHCERCEVNHFGFGPEGCKPCDCHPEGSLSLQC-KDDGRCECREGFVGNRCDQCEENYFYNRSWPGCQECPACY 1035 HSP 5 Score: 99.7525 bits (247), Expect = 9.246e-20 Identity = 120/480 (25.00%), Postives = 203/480 (42.29%), Query Frame = 2
Query: 4613 KKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTL-DLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNE----LIKNATAYLETIK----NIYKEMPNIT--------LAASVLKDVLKYVDELNQIIKYTQ-SHIQSDQINN--LKILKNKTIMADEINTNNKEY--LNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDA--ISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQ 5950
K CNCN Y + C+ VTG C+ C+ + G C C GFY N ++ CE C C + Q R +C+C+ G +G+ CE C+ +G +G CK CDC+ +GS SL C + G C+C G +G +CDQC NY + + C +CY LV+ + V K + L S + +L ++ Q + L + E+ + + Q +KD + ++++ + + L++ I ET + EQ E LI+ A+ LE K N+ P T LA K ++ E + I++ + ++ S + N L+ L + A EI N++Y +S ++ + +R D Q L + + +A I ++ + LI +++ EDL +++ L K+L+ K EQQ
Sbjct: 882 KACNCNLYGTMKQQSSCNPVTGQCE-CLPHVTGQDCGACDPGFY-NLQSGQGCERCDCHALGSTNGQCDIRTG-----QCECQPGITGQHCERCEVNHFGFGPEG--CKPCDCHPEGSLSLQCKDDGRCECREGFVGNRCDQCEENYFYNRSWPGCQECPACYR------LVKDKVADHRV---KLQELESLIANLGTGDEMVTDQAFEDRLKEAER------EVMDLLREAQDVKDVDQNLMDRLQRVNNTLSSQISRLQNIRNTIEETGNLAEQARAHVENTERLIEIASRELEKAKVAAANVSVTQPESTGDPNNMTLLAEEARKLAERHKQEADDIVRVAKTANDTSTEAYNLLLRTLAGENQTAFEIEELNRKYEQAKNISQDLEKQAARVHEEAKRAGDKAVEIYASVAQLSPLDSETLENEANNIKMEAENLEQLIDQKLKDYEDLREDMRGKELEVKNLL----EKGKTEQQ 1333 HSP 6 Score: 97.4413 bits (241), Expect = 4.651e-19 Identity = 93/306 (30.39%), Postives = 125/306 (40.85%), Query Frame = 2
Query: 3005 CFCLENVNGDRCEKCVEGYFN-------YPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNN----RTDCVPCNCGRGS---LNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTG-------CKKCP------PCYNGQVCNNEGICV-CPPNTIGNLCNKCSE----DSWDFNETIGCKKCNCYINGSY--SNICNKISGICACKTAFFGSKCDRCNEGYYGFPN---CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
C C G CE C+ GY Y C C C+ H S CD G C C N C+KC + +Y ++ +DC PC C GS + PK E C + C CD GYFG D G C+ C P G G C+ C NT G C++C + + N CK CNC + G+ + CN ++G C C G C C+ G+Y + C+ C C G+ + + CD TGQC+C+ + G C+ C F G GC C C
Sbjct: 690 CTCPVGYGGQFCEMCLSGYRRETPNLGPYSPCVLCACNGH--SETCDPETGVCNCRDNTAGPHCEKCSDGYYGDSTAGTSSDCQPCPCPGGSSCAVVPKT-KEVVCTNCPTGTTGKRCELCDDGYFG-DPLGRNGPVRLCRLCQCSDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKDGFFGNPLAPNPADKCKACNCNLYGTMKQQSSCNPVTGQCECLPHVTGQDCGACDPGFYNLQSGQGCERCDCHALGSTNGQ---CDIRTGQCECQPGITGQHCERCEVNHF---GFGPEGCKPCDC 985 HSP 7 Score: 90.1225 bits (222), Expect = 8.287e-17 Identity = 65/211 (30.81%), Postives = 100/211 (47.39%), Query Frame = 2
Query: 2840 DKLTGKCDCRTNFVNRQCDSCTSGLYLKDK-----------KCIPCNCEIQGR---ISSEKFCNTT-SGQCF-CLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY---NGQVCNN 3403
DKL C+C+ N C+ C + D+ +C+PC+C + + E + +T G C C +N +G CE+C E +F N C C CSP +LS CD + G+C C V+ CD+C+ + C PC+C +C++ETG C C NV +C +C G+F L+ + + C PC+ + VC N
Sbjct: 301 DKLV--CNCKHNTYGVDCEKCLP--FFNDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGHGGHCTNCQDNTDGAHCERCRENFFRLGNNEACSSCHCSPVGSLSTQCD-SYGRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDPSGSIDECNIETGRCVCKDNVEGFNCERCKPGFFNLESSNPRGCTPCFCFGHSSVCTN 506 HSP 8 Score: 63.1586 bits (152), Expect = 1.257e-8 Identity = 59/199 (29.65%), Postives = 84/199 (42.21%), Query Frame = 2
Query: 4517 NPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGD--CQNCMGNREGNKCQLCREGFYKNDKNY--------DQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFY--GNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY-VVEKKTCQSC 5068
N ++ + E FN P KS + + +C CNG++ C D NC N G C+ C F ND+ + +C PC C S F R C+ G CE C++ F+ GN C SC C+ GS S C+ +G C C PGV+G KCD+C P + + + C+ C
Sbjct: 254 NRLNTFGDEVFNDPKVLKSYYYAISDFAVGG--RCKCNGHASECMKNEFDKLVCNCKHNTYGVDCEKCLPFF--NDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGHGGHCTNCQDNTDGAHCERCRENFFRLGN---NEACSSCHCSPVGSLSTQCDSYGRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPC 445
BLAST of Laminin subunit alpha-2 vs. Ensembl Celegans
Match: lam-3 (LAMinin related. See also lmb; Laminin alpha chain [Source:UniProtKB/TrEMBL;Acc:G5ECE3]) HSP 1 Score: 947.192 bits (2447), Expect = 0.000e+0 Identity = 676/2152 (31.41%), Postives = 1024/2152 (47.58%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT---HKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVA-----VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGD-QKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCK-PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAAT--KEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRG-----------------EVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGD-AKSKNLNSCKKCPCPRLDY---------QMTELCSSYSSE-NYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSAD--CDLNNGKCKCGSNVINRICDKCENLHYWNNRTD-CVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC-YNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTK----CDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIY---EYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSN----LTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKV----------METTQAIGKRVTAVEKCI-CNALTFGNHCQNPISGYY----KEYFNLPNSTKSGSMRTGNRILINLKK-CNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVD-ELNQII-----------KYTQSHIQSDQINNL-----------KILKNKTIMADEINTNNKEYLNTMSTKK-KEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQ-KLDAISKSFQDE-------KDLISINVREIEDLMKNLSSNHLVSKSLI------IPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDIL----AVDYAINQTK---KELDFNRS---NMNIDSLKKLLAEF----------NSNKSEIVENIKNIDKE 6205
L N+ IT++ TCG E +C+LV+ + +++ N S CD+CD N KRHPIE+A D WWQSPS+A GL+++ VN+TIDL+Q YQ+ YI+LKMG+SPRPG W+LE+SLDGE Y PW ++A C F + TT P +DE+ CT+ YS+ L +GEI SL+NGR N L F AR++R R + TL ADLM L++S+ RY+YS+ +I IGG+CIC HA+ C G KC C+HNTCG +C CCPLFNQ PWKP VC++C C ++ C Y+ + + S+ G EGGG C+ C NTEG NCE+C G+YRP + C C+C +G++ +CV + + + QKPGDCICK G+ G C+ C+ GY +P C PCPC+ GSV +F C C CK +V C CK G +L ++NP GC+PC+CFG ++ C+ +I + GW LT TG K P+ + + L + +R ++YYW AP + GN + SYG Y + T +G+ + DV I+ ++ + IE L R+ P G + + K + + L VD+ ++A Y+ Q + ++L H M +E C CP F G SCESC GYRRVNN LY G+CE C C+ +S CD GEC NC HNT G+ CE C G+ G+ ++ L +C++C CP LD ++ L S S+E NYVC C G C+ C +G++ +P C C CN N + +CD TG+C +C +T G C+ CK +G+A +CK C C + G+ + C + G+C+C+ N++ QCD C + C C+C G I S+ C+ SGQC C + V G +C++C YFN+ + C+ C C+ + D CD GKC+C NV +C+KC + ++ D C C C GS + C++ TG C C V C C ++GL GC +C PC GQVC+ +G CVCPPNT+G +C C+ ++WD++ GCK C+C GS +CN +G C CK A+ G KCD C G++ FP C+PC C GT + C++I G+C CK+NV GTKC C +GTF D + GC CFCF + C +V +Y +F E + K I+L P F T P+Y + D + Y F+ + DN+ GL G+R + II F + + + + +D + K + E + + LTR M+AL + + ++ YT I I+ ++ V + TT+AIG VEKC+ C G CQNP GYY +EY N + + LI + C+C+G+S+ C+ T C +C N G+ C+ C G+ + + + C C CP +F+ +C ++ C C+ GY+G+ CE C G+YG+P G C CDC+ GS CN G C+C PGV G C C + + C SC GC L+ +DT+ E + + L R+ +I Q S + I+K ++ ++ K DA E+ R NK ++ FT I E + Q+ +N N T +L+ ++ E T+ + L +L + LN + + Q H +++++ + + LKN+ + ++ + +E + +S K + E +++ +R+ +++ ++ K R +I ++I K+ +E K+ I++ + E+ DL++ + L+ LI T +H E+ ++ ++ + N +A AY NI + AVD A ++ K L S + N +SL+ L + NSN +IVE +K K+
Sbjct: 55 LATNSLITATDTCGQYTAEEYCKLVEHVLLRKTTNTQS-PQCDICDANNVHKRHPIEYA-IDGTRRWWQSPSLANGLRFEKVNITIDLRQEYQVAYIILKMGNSPRPGTWVLEKSLDGEYYEPWQYYAMQDAECMRQF-----GIPATTGV-PRFQKEDEVHCTSEYSKITPLENGEIHTSLVNGRPGAENTSL--------ELQKFTRARFVRLRLISPRTLNADLMIINKKSDSLDKSVTMRYFYSISDISIGGQCICYGHAESCPSDPVTG--QFKCECRHNTCGESCNRCCPLFNQLPWKPGTNSHPNVCQQCQCFNHAHSCVYDDELDRNKWSITPEGVYEGGGRCLECAHNTEGFNCERCKDGYYRPSGLSHYREDACRTCECDPVGSVSDACVRDDQSAENGQKPGDCICKPGFGGRRCERCAPGYRNHPT--CEPCPCNRAGSV--NFDTCDGASCQCKANVEGI-YCDRCKAGTIHLSASNPLGCQPCFCFGHSSTCTQGKWNKAQIINNIGWHLTDLTGGKDVKPEVENGEVLMFNANQNQDR----SLYYWKAPDSFKGNMLNSYGGYLHY--DVYYVPTEQGATVFVADVAIEGNGIKIEYHSRIE--FLPREKMTVAIPMSELSGWYNAEARSPVDKADMMRALANVDRFTVRATYHQPQLQSSIFGLSLDTAVPAPDEIVEEDTSLKALAYHTQDTLMGGVEVCECPENFAGNSCESCVPGYRRVNNQLYGGRCEKCDCHGNSEECDPFTGECLNCRHNTTGSRCELCKPGHVGNPSRGGELGACEQCECPSLDLNPNPECISTELAVLGSVASNEDNYVCINCPLGYEGNKCEYCSDGFFEDPLTG-KCIECTCNGNIDPMGIGNCDSETGKCLKCIGHTTGDSCESCKEHHWGNAQLHTCKPCGCHTQGAVNPQCSEENGECECKENYIGAQCDRCKENHGDVENGCPACDCNDTGSIGSD--CDQVSGQCNCKQGVFGKQCDQCRPSYFNFTDAGCQFCHCNIYGSIEDGKCDQTTGKCECRENVEGTMCEKCADGYFNITSGDGCEDCGCDPTGSEDVSCNLVTGQCVCKPGVTGLKCDSCLPNFYGLTSEGCTECEPCPAPGQVCDPIDGSCVCPPNTVGEMCENCTTNAWDYHPLNGCKLCDCSDIGSDGGMCNTFTGQCKCKAAYVGLKCDLCTHGFFNFPTCEPCGCNAAGTDPLQCKDGQCLCNEI-GECPCKKNVHGTKCDQCGEGTFSLDSSNLKGCTECFCFNRTSNCE---QSDLVWQQMYAEDRRAVFQEPWEFYTKK--HNINLLREKPSHFNSYPTDATPLYWPLPSTMLG-DRTASYNG-FLRFKIWNEDNR----------------RGLHGIRPDQQYFRHFPQVII--FGNNRIELEHIPMEINDDGIYKIRLHESEWRVRHSPELTLTRKQMMVALQDTQGIYIRGTYTYPARGDAINIQEVSLDVAVPESKIVAGLSTTKAIG-----VEKCLGCPQGYTGLSCQNPEVGYYRKKHREYLN----------QADDIALIGWSEPCSCHGHSQTCNPDTSVCTDCEHNTFGDFCEHCLPGYIGDAREGGANACTKCACPLVENSFSDSCVAVDHGRGYVCNSCKPGYTGQYCETCVAGYYGDPQHIGGTCSPCDCHPDGSLHGACNPLSGQCECKPGVTGRTCSMCQERHAFINRVCTSCDQGCYLPLMETMDTMEEHLGRQNFSGLKPIPWKRVWRIGNESQDLSTFVGGIDKDGEI--------VKDSKWAKDAFALLDEVNFQMGRSNKSAVSIKQ-FTGIAEQLILDAQIHYANAF--NTTNFLK----MFAEHGATTVGGAALDGMLMEAEAHLNATVERGEYVEKRLNRAQQEHKKAEELLKMVTAQKLNETIFEDLKNRIDVLEQWMNDYRETIYDVSKKDTADAERMSLVVGKRINRYKEVSNEIEKLRVEAEDQIAYSRNSIEKARSEELMNMFEDKEKINMTLAELPDLVEQCQNITLLYSQLIDEYDEEYVQTAGRHAEKLEVQAQKIVDRFVDTRTETENPLKASHAYENIVEALKNATEAVDSAAEASEAVSKMLGSEGSESGDANEESLRSQLEKLKNESSLSNVDNSNAVKIVEELKKEKKD 2116
BLAST of Laminin subunit alpha-2 vs. Ensembl Celegans
Match: lam-2 (Laminin-like protein lam-2 [Source:UniProtKB/Swiss-Prot;Acc:Q18823]) HSP 1 Score: 433.335 bits (1113), Expect = 1.082e-123 Identity = 326/1059 (30.78%), Postives = 498/1059 (47.03%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAKS---DDDNLWWQSPSMAQGLKY-QNVNVTIDLKQVYQIVYILLKMGDSPRPGNW-ILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKC-DKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEE-----CNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDL-CKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAK-NLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDK------AATKEEFISCLVKVDKLLIKAKYYSNQKSIELL-NITLSRGEVHLAL---DEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQG---QCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTK---SCKKCDCDSLGSTSEN-------CDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCS-PHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCN 3232
N ++ + TCGT P FC QR + C+ CD+R+E HP ++ ++ WWQS +M +G +Y N+T+ L + + I Y+ LK SPRP ++ I +++ + PW F++ + + Y L R P L P + A + CT +S + G I S + GR S E + L +++A IR + T ++ + + + YYY++ + +GG+C CN HA +C +G + C C+HNT G++C C P +N RPW+ E CNC++ SN C ++Q + + G GG CI+CQ NT+G++CE+CI +R E Y C C C+ +G++ + N G C CK G G CD C G+ + C C C GS+ +C +N + C CK GYF+L + N GC PC+CFG ++ C++ + +S + + G+ + T + L + D N+P Y+ AP +LG+ +SY + +K+ T + D D+II D R S SI Q F + ++ ++ D + +FI L + + I+ Y + K I L N+ L V + + IE C C PGF G CESCESG+RR + G C C C+NHSN+C++ +G C C HNTAG CE CA GYYGDA C+KCPCP C ++ + +C +C + +G CD C +GY+GNPK C C C+ N + +CD +TGEC +C +NT G C+ CK G++GDAL + +C+ C C + G+ N C++ G+CDC N + QCD C G Y C CNC+ G S C+ +GQC C V G RC++C + +F + C+ C C + + CD+N+G+C C NV R CD+C Y + C+PC+
Sbjct: 61 NLEVQVTNTCGTKRPTKFCVQSGHTG-QRSV-------CETCDDRHEGFSHPAKYLTDFNVGNNETWWQSDTMQEGQQYPTTTNLTLVLGKSFDITYVRLKF-ISPRPESFTIYKKTHTDSEWEPWQFYSGSCRATYGLSDRA-PIL-------PGNEATAQ--CTKEFSDISPITGGNIAFSTLEGRPSAHAFEESEV------LQKWVTASAIRISLNRMNTFGDEV-----FKDPQVLRSYYYAISDFAVGGRCKCNGHASECVGSSSVDGENRLVCRCEHNTQGADCNECLPFYNDRPWRSGTSVEANECIACNCSQLSNRCYFDQQL------FEETGH---GGHCIDCQGNTQGVHCEQCIANHWR----RPGENY-CVACGCNEIGSLSTQCDN--------EGKCQCKPGVTGRFCDQCLDGFYDFSTNGCKNCGCETSGSLNNQPRCDSSSGSCSCKLNVEGRQCDKCKPGYFDLSTENQFGCTPCFCFGHSSICNTADGYFAMNVSSVFDQDKQKWAGQNRIGLQDTQWAELDKAVAVSDTD-NSPV--YFVAPEQFLGDQRSSYNQDLVFTLKVAKHVTNQ----DVKDIIIVGAD-RQELSTSI---TAQGNPFPTTEAQTYRF-RVHADPYFGWYPRINELDFIGILSNITAIKIRGTY--SYKDIGYLSNVNLGTAGVAPSAANPKQATWIEHCECLPGFVGQFCESCESGFRR--ETKFGGPFNHCIKCDCHNHSNSCEAESGSCI-CEHNTAGDTCERCARGYYGDALQGTEEDCQKCPCPN-----DGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDALIEPKGNCQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSGLGCQECNCDPLG--SEGNTCDVNTGQCQCKPGVTGQRCDRCADYHFGFSANGCQPCDCEYIGSENQQCDVNSGQCLCKENVEGRRCDQCAENRYGITQG-CLPCD 1042 HSP 2 Score: 159.458 bits (402), Expect = 2.661e-38 Identity = 122/373 (32.71%), Postives = 175/373 (46.92%), Query Frame = 2
Query: 2858 CDCRTNFVNRQCDSCTSGLYLKDK------KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN------YPNCKKCLC---SPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPCNCGRGSLNPK----CDMETGNCK-CAQNVARRDCSKCDVGYFG---LDVTG-CKKCPPCYNGQV-----------CNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRI 3856
C+C FV + C+SC SG + K CI C+C + C SG C C N GD CE+C GY+ +C+KC C P L AD D+ +C G R CD+C + ++ N + T+CV C C G+ +P CD TG CK C N +C C GY+G ++ G C+ C C+ CN + G C C PN IG C++C+ ++ +GC++CNC GS N C+ +G C CK G +CDRC + ++GF CQPC C G+ E +CD +GQC CK+NV G +C C + + GC C + C+ ++ R+
Sbjct: 702 CECLPGFVGQFCESCESGFRRETKFGGPFNHCIKCDCH-----NHSNSCEAESGSCICEHNTAGDTCERCARGYYGDALQGTEEDCQKCPCPNDGPCILHADGDVICTECPNG--YTGRRCDECSDGYFGNPKDGTECVECACS-GNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDALIEPKGNCQSCG-CFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSGLGCQECNCDPLGSEGNTCDVNTGQCQCKPGVTGQRCDRCADYHFGFSANGCQPCDCEYIGS---ENQQCDVNSGQCLCKENVEGRRCDQCAENRY----GITQGCLPC------DDCYTLIQSRV 1052 HSP 3 Score: 147.517 bits (371), Expect = 9.548e-35 Identity = 110/347 (31.70%), Postives = 162/347 (46.69%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGY-----YGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALT---KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK---KCIPCNCEIQGRISSEKFCNTTSGQC-FCLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSA--------DCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNG---QVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC-NCY 3514
+CL G C+ C G+ +G P C +C C+N+ SC+ +G C C +NT G C+ C G+YGDAL + C+KC C + G + D +C + R+CD C+ G + K +C+ C C +S C+ +G+C C+ N +G CE C GY+ NC+ C C +C+ +G+C C NVI CD+C + Y + C CNC GS CD+ TG C+C V + C +C +FG GC+ C Y G Q C+ N G C+C N G C++C+E+ + T GC C +CY
Sbjct: 703 ECLPGFVGQFCESCESGFRRETKFGGPFNH--CIKCDCHNHSNSCEAESGSCICEHNTAGDTCERCARGYYGDALQGTEEDCQKCPCPNDGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDALIEPKGNCQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSGLGCQECNCDPLGSEGNTCDVNTGQCQCKPGVTGQRCDRCADYHFGFSANGCQPCDCEYIGSENQQCDVNSGQCLCKENVEGRRCDQCAENRYGI--TQGCLPCDDCY 1045 HSP 4 Score: 91.2781 bits (225), Expect = 1.300e-17 Identity = 121/575 (21.04%), Postives = 234/575 (40.70%), Query Frame = 2
Query: 4616 KCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKND--KNYDQCEPCQCPSAHYNFAQN-CTRIE-SSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAP-NYVVEKKTCQSC---YSG--------------CTKDLL-VRIDTLNETVINFKWKTLP--STLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDI-NETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITL-AASVLKDVLKYVD---ELNQIIKYTQSHIQSDQINNLKILKNKTI--------MADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDIL--AVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDK 6202
+C C+G + CD +TG+C+ C+ N G C+ C+ G++ + + C+ C C +A N T +E + + +C C G C+ C GFY N G+ C+ C+C+ GS+ C+ G CQC PGV G +CD+CA ++ CQ C Y G C +++ R D E LP L +++N+ + L N++ + + + + +K+K+ ++ E +K + + +++ I +E ++ E+L +E + A + +N K I A +++VL Y++ E I Y S +Q + L + T A EI +++ + + KE I + K + ++ K + +++ + + + KS + +L ++++ +E V + P L+ V L +S NL+ K+ N D L V+ ++ + EL ++ + +L+ E ++ IV+++ DK
Sbjct: 834 ECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDALIEPKGNCQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFY-NITSGLGCQECNCDPLGSEGNTCDVNTGQCQCKPGVTGQRCDRCADYHFGFSANGCQPCDCEYIGSENQQCDVNSGQCLCKENVEGRRCDQCAENRYGITQGCLPCDDCYTLIQSRVNVFREKVKSLDNTLQEIIENPAPVNDTKFD-EKVKETSRAASEVWEAVKQKTKEGGGTIKTKSKAIKDEIVAALEKLTSIDESVAQAKVGADAAENDMKRWEIIIENARREIENVLHYLETEGEERAQIAYNASQKYGEQSKRMSELASGTREEAEKHLKQASEIEQLSEQAIANATQANKEASDAIYGGEQISKQIAELKEKQNQLNESIHRTLDLAEEQKKSADEANNLAAVSLTNVE----------AVKIPSVDPKELRNDVAGVLEESENLVDSSVKE---------------NSANDELFDEVNRSVADARNELQSSQDQQRVSD--QLMLELEKSRERIVDSVSTADK 1379 HSP 5 Score: 80.8777 bits (198), Expect = 1.668e-14 Identity = 77/268 (28.73%), Postives = 120/268 (44.78%), Query Frame = 2
Query: 4328 FMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPS-----AHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDC--NQQGSQSLNCNEF-GIC-QCSPGVLGIKCDQCAPNY-----VVEKKTCQSCYSGC 5080
F+ L N+T++K++ Y+ K L + + T V + A K+ T +E C C G C++ SG+ +E TK G + KC+C+ +S C++ +G C C N G+ C+ C G+Y + + C+ C CP+ H + CT +C GY+G+ C+ C G++GNP G C C C N + NC++ G C +C G C+ C P Y + K CQSC GC
Sbjct: 652 FIGILSNITAIKIRGTYSYKDIGYLSNVNLGTAGVAPSA-ANPKQATWIEHCECLPGFVGQFCESCESGFRRE-------TKFGGP------FNHCIKCDCHNHSNSCEAESGSCI-CEHNTAGDTCERCARGYYGDALQGTEEDCQKCPCPNDGPCILHADGDVICT----------ECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDALIEPKGNCQSC--GC 892 HSP 6 Score: 80.1073 bits (196), Expect = 3.242e-14 Identity = 66/212 (31.13%), Postives = 95/212 (44.81%), Query Frame = 2
Query: 3245 SLNPKCDMETGNCKCAQNVARRDCSKCDVGY-----FGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSE----DSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP----NCQPCSCAENGTKSNELTKCDKITGQC-QCKQNVIGTKCKVCRKGTFPSDGTYE--GGCFSCFCFGVSEK 3829
+ NPK +C+C + C C+ G+ FG C KC + C E G C+C NT G+ C +C+ D+ E C+KC C +G + C + G +CD C++GY+G P C C+C+ N T N + CDKITG+C +C N G C+ C+ G + D E G C SC CF +
Sbjct: 690 AANPKQATWIEHCECLPGFVGQFCESCESGFRRETKFGGPFNHCIKCDCHNHSNSCEAESGSCICEHNTAGDTCERCARGYYGDALQGTEE-DCQKCPCPNDGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGN-TDPNSIGNCDKITGECKKCIFNTHGFNCENCKPG-YWGDALIEPKGNCQSCGCFAAGTR 898 HSP 7 Score: 77.7962 bits (190), Expect = 1.401e-13 Identity = 85/269 (31.60%), Postives = 111/269 (41.26%), Query Frame = 2
Query: 2660 RCKCNNNQESC---DPVTGE----CRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNC-EIQGR--ISSEKFCNTT-SGQCF-CLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLN--PKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY---NGQVCN 3400
RCKCN + C V GE CRC +NT G +C C FY D +S G ++ +CI CNC ++ R + F T G C C N G CE+C+ ++ P C C C+ +LS CD N GKC+C V R CD+C + Y + C C C GSLN P+CD +G+C C NV R C KC GYF L C PC+ + +CN
Sbjct: 286 RCKCNGHASECVGSSSVDGENRLVCRCEHNTQGADCNECLP-FYNDRPWRS---------------------------------------GTSVEANECIACNCSQLSNRCYFDQQLFEETGHGGHCIDCQGNTQGVHCEQCIANHWRRPGENYCVACGCNEIGSLSTQCD-NEGKCQCKPGVTGRFCDQCLDGFYDFSTNGCKNCGCETSGSLNNQPRCDSSSGSCSCKLNVEGRQCDKCKPGYFDLSTENQFGCTPCFCFGHSSICN 513 HSP 8 Score: 72.4034 bits (176), Expect = 6.701e-12 Identity = 62/213 (29.11%), Postives = 90/213 (42.25%), Query Frame = 2
Query: 4478 CICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGD--CQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPS-AHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPI---KGIPCKSCDCNQQGSQS-------LNCNEF-GICQCSPGVLGIKCDQCAPNY--VVEKKTCQSC 5068
CIC T G+ C+ GYY + + +KC C GD C C G +C C +G++ N K+ +C C C N NC +I + +C G +CENC+ G++G+ + KG C+SC C G++ L CN+ G C C P V+GI+CDQCA + + CQ C
Sbjct: 752 CICEHNTAGDTCERCARGYYGDALQGTEE--------------DCQKCPCPNDGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGNTDPNSIGNCDKITGECK---KCIFNTHGFNCENCKPGYWGDALIEPKG-NCQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSGLGCQEC 946 HSP 9 Score: 70.8626 bits (172), Expect = 1.982e-11 Identity = 76/287 (26.48%), Postives = 116/287 (40.42%), Query Frame = 2
Query: 3281 CKCAQNVARRDCSKC-----DVGYFGLDVTGCKKCPPCYNGQVCN-------------NEGICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLN--ANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSV 4072
C+C N DC++C D + +C C Q+ N + G C+ C NT G C +C + W C C C GS S C+ G C CK G CD+C +G+Y F C+ C C +G+ +N+ +CD +G C CK NV G +C C+ G F + GC CFCFG S C+ + + S +FD+ +G++ + A + T P+Y + F S + + + + V
Sbjct: 310 CRCEHNTQGADCNECLPFYNDRPWRSGTSVEANECIACNCSQLSNRCYFDQQLFEETGHGGHCIDCQGNTQGVHCEQCIANHWRRPGENYCVACGCNEIGSLSTQCDN-EGKCQCKPGVTGRFCDQCLDGFYDFSTNGCKNCGCETSGSLNNQ-PRCDSSSGSCSCKLNVEGRQCDKCKPGYFDLSTENQFGCTPCFCFGHSSICNTADGYFAMNVS----SVFDQDKQKWAGQNRIGLQDTQWAELDKAVAVSDTDNSPVYFVAPEQFLGDQRSSYNQDLVFTLKV 590 HSP 10 Score: 55.8398 bits (133), Expect = 6.957e-7 Identity = 41/146 (28.08%), Postives = 60/146 (41.10%), Query Frame = 2
Query: 2246 KCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHK------CLHNTSGVHCDRCIEGYYGNPKKKISCKRC 2665
+C+C P G+ C+ C G+ + + L +C + NTCD G+CQ C G C+ CA ++G + N C+ C C + SEN C C N G CD+C E YG + + C C
Sbjct: 915 QCDCLPNVIGIQCDQCAHGFYNITSGLGCQECNCDPLGSEGNTCDVNTGQCQ-CKPGVTGQRCDRCADYHFG----FSANGCQPCDCEYI-----------GSENQQCDVNSGQCLCKENVEGRRCDQCAENRYGITQGCLPCDDC 1044
BLAST of Laminin subunit alpha-2 vs. Ensembl Celegans
Match: epi-1 (Laminin-like protein epi-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21313]) HSP 1 Score: 294.278 bits (752), Expect = 6.082e-79 Identity = 184/561 (32.80%), Postives = 273/561 (48.66%), Query Frame = 2
Query: 95 RIFIIFILLIVHKAHSQDDEPEILSVNVDGLLMENAKITSSATCG--TGGP--EIFCRLVDEIAIQRL-----------------INP---DSHVLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSA 1687
+ + + L+ +SQ P ++++ IT+++TCG G P EI+C L L NP H C C+ NE HP D +N WW SP +++GL++ VN+TIDL+Q + + Y+ ++M +SPRPG+W+LERS D G+ Y PW FAE C F + + P S DD + C T + + L + E++I ++ R S + ++ + + L NF A +R R L TL LM + + ++ RY+Y+++ I IGG+C+CN HA CD + C C+HNTCG CE CCP F Q+ W+ CE CNC +SNEC Y+ V + S++ G EGGGVC NC+ENTEG+NC KC G++RP V E PC C C G+C ++ G C C + G +CD C+ GY P +C PC C+++G++ C CK C+TC +GY N+ + GC C C ++ + SA
Sbjct: 12 KALFLIVTLLAQFTYSQVLTPSQITIS------HRKPITATSTCGEIQGQPVTEIYCSLTGSTQYTPLNSYSYQDDEQQKSWSQYENPMVRGGHG-CGHCNAGNENS-HPAA-NMVDGNNSWWMSPPLSRGLQHNEVNITIDLEQEFHVAYVWIQMANSPRPGSWVLERSTDHGKTYQPWFNFAENAAECMRRFGME--------SLSPIS-EDDSVTCRTDMASLQPLENAEMVIRILEHRPS--SRQF----ATSEALQNFTRATNVRLRLLGTRTLQGHLMDMNEWRDPTVTRRYFYAIKEIMIGGRCVCNGHAVTCDILEPQRPKSLLCRCEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGACA--------EETGKCECLPRFVGEDCDQCASGYYDAP--KCKPCECNVNGTIGDVCLPEDGQCPCKAGFGG-TFCETCADGYTNVTA----GCVECVCDATGSEHGNCSA 533 HSP 2 Score: 285.419 bits (729), Expect = 2.596e-76 Identity = 225/745 (30.20%), Postives = 324/745 (43.49%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGF--YRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQC-SSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPD-KAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHL--ALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCH-KCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNN-----QESCDPVTGECR-CSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGL--YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKC 3184
+C CK G CE C P + P C +C CN +C E G C C + EG C++C+ Y P L C C CH G+ G G + D + G C+C++ G CD C G+ +P+ C C C+ G+ C CK +V C+ CK G F+L + NP GC C+CFG+T+ C SS+ + D+ + T G + K + Y + E S P Y+ P ++ TSYG + + + K D+ DV + + + + S + + Q + K LT + K T+EE + L + + +KA Y+ + K+ L L E + ++ + +E+C CP + G SC+ C SGY RV + + G C C C HS TCD G C +C HNT G HCE C G+YG+A + + C C CP S E + C +G CDRC G++G+P+ SC C+CN N SC P +G+C C NT G C+ C FYGDA+T K+C C+C G S+ CD +G C+C+ N CD C + K + C C+C G + CN +GQC C G RCE C GY+NY C KC C ++ CD+ G+C C CD+C
Sbjct: 1443 GQCKCKPGVIGRRCERCAPGYYNFPE-----CIKCQCN-AGQQCD------------------ERTGQCF-CPPHVEGQTCDRCVSNAFGYDP-------LIGCQKCGCHPQGSEG-----GNLVCDPESGQCLCRESMGGRQCDRCLAGFYGFPH--CYGCSCNRAGTTEEICDATNAQCKCKENVYGG-RCEACKAGTFDLSAENPLGCVNCFCFGVTDSCRSSMYPVTIMSVDMSSFLTTDDNG--MVDNKDDTVIYTSEETS--------PNSVYFNVPIEK-KDYTTSYGLKLTFKLSTVPRGGRKSMNADA-DVRLTGANMTIEYWASEQPTNPEEQFTVKCKLVPENF--LTAEGKTVTREELMKVLHSLQNITLKASYFDHPKTSTLYEFGLEISEPNGVDSVIKASSVEQCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECSQCG--SQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHC---GTAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQC 2128 HSP 3 Score: 241.891 bits (616), Expect = 3.854e-63 Identity = 167/515 (32.43%), Postives = 237/515 (46.02%), Query Frame = 2
Query: 2339 CEACACYNHSNTCD-----SLN-------------GECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQ---ESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHAL-SADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGL---------------DVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWD--FNETIGCKKCNCYINGSYS--NICNKISGICACKTAFFGSKCDRCNEGYYGFPN-----CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKC 3742
CEAC C+ SN C+ LN G C+NC NT G +C C+ GY+ + + + + PC D + + + E C +CL G CD+C GYY PK CK C+CN N + C P G+C C GT C+ C G+ +T C +C CD+ GS NC TG+C+C+ + CD C G + D C CNC+ G + C+ T+GQC C E GD+C++C ++ YPNCK C C + S +CD +G+C C N R CDKC Y N DC C C G+ CD G C C N C +C ++ D GC K P G++C+ C + G +C++C WD ++ GC+ C+C +NG+ S N C+ +G C CK G +CD+C +G+Y + C+ C C G E CD +GQC+C+ V G +C
Sbjct: 354 CEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYF---RPEGVTWNEPQPCKVCDCDPDKHTGACAEETGKC-ECLPRFVGEDCDQCASGYYDAPK----CKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTN--VTAGCVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGYFGDD--CKFCNCDPMG--TEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFY--NYPDCRGCECLLSGAKGQTCD-SNGQCYCKGNFEGERCDRCKPNFYNFPICEECNCNPSGVTRDFQGCDKVSP---GELCS------CRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAE---CDITSGQCKCRPRVTGLRC 839 HSP 4 Score: 240.736 bits (613), Expect = 9.784e-63 Identity = 188/633 (29.70%), Postives = 283/633 (44.71%), Query Frame = 2
Query: 3221 VPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSN--ICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLN-ANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKN---YDQCEPCQCPSAHY-NFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQG----SQSLNCNEFGICQCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
+ C+C +GS + +C+ G CKC V R C +C GY+ + C KC C GQ C+ G C CPP+ G C++C +++ ++ IGC+KC C+ GS +C+ SG C C+ + G +CDRC G+YGFP+C CSC GT + E+ CD QC+CK+NV G +C+ C+ GTF GC +CFCFGV++ C + + + + + + N + KD + I+ + P + + + + Y L + LS R +++ D +V + N + Y +E N + + + + F+ E V L + + +L N+T LK + L + + + I + ++VE+C C A G CQ SGY++ +SGS L C CNG+S CD TG C +C N G+ C+ C EG Y N N YD C C CP A NFA++C E L+C C+ GY+G C+ C GF+G+P I G C C CN S+S + N C G C+ CA + V K C SC
Sbjct: 1424 LSCDCVAQGSESFQCEQYGGQCKCKPGVIGRRCERCAPGYY--NFPECIKCQ-CNAGQQCDERTGQCFCPPHVEGQTCDRCVSNAFGYDPLIGCQKCGCHPQGSEGGNLVCDPESGQCLCRESMGGRQCDRCLAGFYGFPHCYGCSCNRAGT-TEEI--CDATNAQCKCKENVYGGRCEACKAGTFDLSAENPLGCVNCFCFGVTDSCRSSMYPVTIMSVDMSSFLTTDDNGMVDNKDDTVIYTSEETSPNSVYFNVPIEKKDYTTSYGLKLTFKLSTVPRGGRKSMNAD-------------ADVRLTGAN--MTIEYWA--------------SEQPTNPEEQFTVKCKLVPENFLTAEGKTVTREELMK--VLHSLQNIT-LKASYFDHPKTSTLYEFGLEISEPNGVDSVI--KASSVEQCQCPAPYTGPSCQLCASGYHR--------VQSGSF------LGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYD-CMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSC 2001 HSP 5 Score: 221.09 bits (562), Expect = 9.145e-57 Identity = 168/528 (31.82%), Postives = 243/528 (46.02%), Query Frame = 2
Query: 2348 CACYNHSNTCDSLNGE------CQNCTHNTAGAHCEDCASGY----YGDAKSKNLNSCKKCPC----------PRLDYQMTELCSSYSSENY-VCHKCLHNTSGVHCDRCIEGYYGNPK-----KKISCKRCKCNNNQE--SCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGL-DVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNI--CNKISG--ICACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
C C H+ TCD L + C+ C HNT G CE C G+ + A + N +C+ C C +D + S + E VC C NT GV+C++C GY+ P+ + CK C C+ ++ +C TG+C C +G +C C G+Y DA CK C+C+ G+ + C G+C C+ F C++C G C+ C C+ G S C+ ++GQC C G C+KC GYF +CK C C P CD G+C C CD+C+ Y +C C C G G +P+CD +G C C N R C KC G++ D GC+ GQ C++ G C C N G C++C + ++F C++CNC +G + C+K+S +C+C+ G CD+C ++ C+ C C NGT S L CD TGQC CK+N G +C C G + + + GC SC C
Sbjct: 298 CVCNGHAVTCDILEPQRPKSLLCR-CEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYF-RPEGVTWNEPQPCKVCDCDPDKHTGACAEETGKCECLPRFVGEDCDQCASGYY-DA--PKCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVECVCDATG--SEHGNCSASTGQCECKPAYAGLSCDKCQVGYFG-DDCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFY--GYPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYNYPDCRGCECLLSGAKGQTCDSNGQCYCKGNFEGERCDRCKPNFYNFPI---CEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISG-LNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHC 811 HSP 6 Score: 180.259 bits (456), Expect = 1.942e-44 Identity = 138/434 (31.80%), Postives = 205/434 (47.24%), Query Frame = 2
Query: 2246 KCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHS---NTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCN---NNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD---CD-LNNGK-CKCGSNVINRICDKCENLHY---WNNRTDCVPCNC---GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV---TGCKKCPPCYNGQVCNNE-----GICVCPPNTIGNLCNKCSEDSW 3472
KC C P F G C+ C SGY +C+ C C + + C +G+C C G CE CA GY + C +C C + CS+ + + C +C +G+ CD+C GY+G+ CK C C+ CD TG+C C G +C C FYG +CK C CD G TS CD +G+C C NF R CD C +G Y C C C + G + + C+ ++GQC+C N G+RC++C ++N+P C++C C+P ++ D CD ++ G+ C C +V RICD+C+ + +++ C C+C G S CD++TG C C +N R C +C G++ L+ GC+ C C G E G C C P G C++ E+ +
Sbjct: 444 KCECLPRFVGEDCDQCASGYYDAP------KCKPCECNVNGTIGDVCLPEDGQCP-CKAGFGGTFCETCADGY-----TNVTAGCVECVCDATGSEHGN-CSASTGQ---C-ECKPAYAGLSCDKCQVGYFGD-----DCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYG---YPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFY-NYPDCRGCECLLSG--AKGQTCD-SNGQCYCKGNFEGERCDRCKPNFYNFPICEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESC-HCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENHY 847 HSP 7 Score: 163.696 bits (413), Expect = 1.901e-39 Identity = 129/418 (30.86%), Postives = 186/418 (44.50%), Query Frame = 2
Query: 2624 YYGNPKKKISCKRCKCNNNQESCD------PVTGECRCSYNTMGTECQLCKHGFYG---DALTK----SCKKCDCDSLGSTSE-------NCDKLT----------GKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCR 3754
Y+ K+ + RC CN + +CD P + CRC +NT G C+ C GF A T +C+ C+C G ++E + +K + G C +CR N C+ C+ G + + + C C+C+ + C +G+C CL G+ C++C GY++ P CK C C+ + D C +G+C C + C+ C + Y N CV C C GS + C TG C+C A C KC VGYFG D C P G VC+ G C+C G+ C++C + + CK C C G S C+ SG C C F G CD+C G+Y +P+C+ C C +G K CD GQC CK N G +C C+
Sbjct: 285 YFYAIKEIMIGGRCVCNGHAVTCDILEPQRPKSLLCRCEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNC--FGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGA---CAEETGKCECLPRFVGEDCDQCASGYYDAPKCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCAD-GYTNVTAGCVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGYFGDDCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPN---CKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYNYPDCRGCECLLSGAKGQ---TCDS-NGQCYCKGNFEGERCDRCK 689 HSP 8 Score: 148.288 bits (373), Expect = 8.027e-35 Identity = 118/387 (30.49%), Postives = 169/387 (43.67%), Query Frame = 2
Query: 2231 MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHK------CLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNN---NQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTS-GQ-CFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPHALSAD---CDLNNGKCKCGSNVINRICDKCENLHYW---NNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSK 3322
+P +C C GF G CE+C GY V + C+A H N C + G+C+ C AG C+ C GY+GD CK C C + +E VC + C +G CDRC +YG P +CK C C+ CD +G+C C+ N G C C GFY C+ C+C G+ + CD G+C C+ NF +CD C Y C CNC G + C+ S G+ C C ++V G C++C +++ C+ C C+ + + CDL G+C C N R CD+C + Y N+ C C+C G+L +CD+ +G CKC V C +
Sbjct: 485 LPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVECVCDATGS-EHGN-CSASTGQCE-CKPAYAGLSCDKCQVGYFGD-------DCKFCNCDPM-----------GTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYP----NCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYN---YPDCRGCECLLSGAKGQTCDS-NGQCYCKGNFEGERCDRCKPNFY-NFPICEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQ 841 HSP 9 Score: 144.821 bits (364), Expect = 8.543e-34 Identity = 102/338 (30.18%), Postives = 155/338 (45.86%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPKKKI--SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGST--SENCDKLTG----KCDCRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR-TDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCN 3508
+C +G C C GY+ +C C+CN + +CDP TG C C +NT G C+ C G YG+A S C C C + +++CD +C+C+ + +CD C SG + + + C PC C ++ + C+ SG C+ C +N +G CE C ++ NC C CS S CD +G C+C NV CD+C+ H+ ++ C C+CG + N +C++E G C C C C+ GY+ GC KC G VC+ G C C G+ C++C S+ T GC++C+
Sbjct: 1809 QCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECS-QCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCGTAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQCLP-SYLRIPTYGCRRCD 2144 HSP 10 Score: 136.732 bits (343), Expect = 3.000e-31 Identity = 108/369 (29.27%), Postives = 154/369 (41.73%), Query Frame = 2
Query: 2708 ECRCSYNTMGTECQLCKHGFY---GDALTKSCKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKDK-----KCIPCNCEIQGRISSEKFCNTTSG----QCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY----NGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGY--YGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
+C+C G CQLC G++ + +C C+C+ +T CD TG C DC N C+ C G Y C+ C C + K C+ + QC C GDRC++C G+F +P CSP C+C N NN TD + C +G+C C QN R C C ++G VT K C C Q C+N+ G C C N G+ C++C D W F++ GC+ C+C +++ CN +G C C+ G +C+ C GY YG C C C + + T CD TGQC C++ G++C C
Sbjct: 1807 QCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSAT---CDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSP-------------CQCNGN---------------NNLTD-----------SRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTA-KNCSSCECSQCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCG-TAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMG---TVCDVRTGQCHCQEGATGSRCDQC 2128 HSP 11 Score: 103.605 bits (257), Expect = 3.119e-21 Identity = 92/299 (30.77%), Postives = 123/299 (41.14%), Query Frame = 2
Query: 3002 QCFCLENVNGDRCEKCVEGYFNYPN------CKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWNNRT----DCVPCNCGRGSLN---PKCDM-ETGN---CKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY--------NGQVCN-NEGIC-VCPPNTIGNLCNKCSEDSWDFNETIGCKKCN-CYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
QC C G C+ C GY + C C C+ H SA CD + G C C N C+ C HY N DC+ C C N CD+ E G C C C +C G+FG + C PC + + C+ N G C +C NT G C C+ +W + + + K C+ C + S C+ SG C CK G CDRC ++GF CQ C GT + T+C+ GQC C+ G +C+ C G + E GC C C
Sbjct: 1807 QCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGH--SATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCA--AWFYGDAVTAKNCSSCECSQCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCGTAAFN-TQCNVENGQCTCRPGATGMRCEHCEHGYW---NYGEHGCDKCDC 2097 HSP 12 Score: 100.523 bits (249), Expect = 2.536e-20 Identity = 163/813 (20.05%), Postives = 311/813 (38.25%), Query Frame = 2
Query: 4481 ICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSCYS------GCTKDLLVRIDTLNETVINFKWKTLPSTLDLR----LNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQ----KMKDDAKEKYGE-----------IEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEM--PNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDA----ISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLN---------KSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDI----LAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIP-----------TGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRN---LEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELI----HGAAARSVE---PIRHVVRCVRHY-VTYYLPTSNAFSLEFYM 6712
+C T G HC++ + +Y + N + C C+ S+ CD+ +G C+ C N EG+ C C+ + K C+ C C +A +N N +E+ +C CR G +G CE+C+ G++ G C CDC S C+ G C C G G +CDQC P+Y+ + C+ C G +L + ID L + N T+ R N IN +M ++ NS + +I S Q Q + K ++ ++++ R + + +RS S + + + L+K A L T++ + L+++ K + E + + + ++ + K +A +N+ + + S K + + +L ++++L ++ + + + QKL+ I+++ +D+++ ++ V + + +++ +K + D +K V+ QL +++ L + + + A +A A+SN+T + +D A E F S N K E + + + KE EKL KQ + + +KL+ E + + IP +VE LK + E + + + L RN L KL V+ +++ +L SS K+ + + ++E + AR + I H S++ P R V R H +++Y T + +
Sbjct: 1971 LCEQNTDGRHCESCAAWFYGDAVTAKNCSS----------------CECSQCGSQYCDNKSGGCE-CKINVEGDSCDRCKPDHWGFSK-CQGCQGCHCGTAAFNTQCN---VENG---QCTCRPGATGMRCEHCEHGYWNYGEHG--CDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQCLPSYLRIPTYGCRRCDECVHHLIGDVDNLELEIDVLGTAIANISSATIVGARLARNKKEFNDINEITKMLNDEENSFGNVFGDAQDILTNSTQIQNKLVRTKTHSQNSVSSAKNITLNGTEFLQEVMKRAQRARQSVRSLAEIALAIGSSSKAVNVDPRLLKEAEETLMTLEAASADQYPEKAQTVPGKLEEIQKKIQEETEKLDKQKETFEAQK-------KRAEELAAYLNSAQQLLKESKSKADKSNNIAKMLQLTKVENLVAAITDDLERVEAAKGEFQKLNVAIGNITENLKDKREEMTHAVTTLNETRNDVAEALEAAKKRVRRD--EKSVDMQLVNAKAHELHLQATTLRQTFDNNKDNTDQAVEAANAFSNLTDTLKNAKAQIDNAYEALSAEPAFAESVQNARD-KPFPDETKEKIDALSKTVSQDLKETEKLKKQLEQLTELSEKLRKRKEAVKAGIPKYSKNTLDSIDEKVQEVEKLKAEIDANIEETRAKISEIAGKAEEITEKANSAMEGIRLARRNSVQLNKLAPVIVSKFEELKKLSSARSAKVDSVSDKVSQIKEMIAVARDAANRIKLGAHFEKGSSLDLNIPQR-VTRSAAHADISFYFRTEQEHGIPLFF 2746 HSP 13 Score: 70.0922 bits (170), Expect = 3.417e-11 Identity = 52/166 (31.33%), Postives = 72/166 (43.37%), Query Frame = 2
Query: 4613 KKCNCNGYSK---VCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSC---YSGCTKDL 5092
K CNC+ VCD TG C C G+KC C FY Y C+ C C A + C +C C ++G++C+ C GFY P C+ C+C G++ C+ G C C G +CD+C PN+ C+ C SG T+D
Sbjct: 562 KFCNCDPMGTEGGVCDQTTGQCL-CKEGFAGDKCDRCDIAFY----GYPNCKACACDGAGIT-SPECDATSG----QCPCNGNFTGRTCDKCAAGFYNYP----DCRGCECLLSGAKGQTCDSNGQCYCKGNFEGERCDRCKPNF-YNFPICEECNCNPSGVTRDF 712 HSP 14 Score: 67.0106 bits (162), Expect = 3.631e-10 Identity = 59/204 (28.92%), Postives = 87/204 (42.65%), Query Frame = 2
Query: 1004 SVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYER---YPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYF 1606
S +C CK N G C+ C P F P +CEECNCN + + ++ G +C +C+++ G C++C F W++ C C C+ GTI +G + D K G C+CKK +G CD C+ G+ R Y C C C I G++R C C+ V + +N YF
Sbjct: 670 SNGQCYCKGNFEGERCDRCKPNFYNFP-----ICEECNCNP-----------SGVTRDFQGCDKVSPGELC-SCRKHVTGRICDQCKPTF---WDLQYHHEDGCRSCDCNVNGTI-----SGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENHYF 848 HSP 15 Score: 65.4698 bits (158), Expect = 9.206e-10 Identity = 65/208 (31.25%), Postives = 91/208 (43.75%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIP-CKSCDCNQQGSQS-LNCNEF--GICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCY 5071
+C CN G C +G+Y N P+ + +G + + CDS G C C GN EG +C C+ FY N+ CE C C PS Q C ++ S EL C CR +G+ C+ C+ F+ C+SCDCN G+ S LN + G C C G +CDQCA + + C+SC+
Sbjct: 628 QCPCNGNFTGRTCDKCAAGFY----NYPDCRGCECLLSGAK-------------GQTCDS-NGQCY-CKGNFEGERCDRCKPNFY----NFPICEECNCNPSGVTRDFQGCDKV-SPGEL-CSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCH 810 HSP 16 Score: 65.0846 bits (157), Expect = 1.309e-9 Identity = 44/141 (31.21%), Postives = 64/141 (45.39%), Query Frame = 2
Query: 4619 CNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
C+ + ++ C TG C+ C+ G C C G+Y K C+PC+C N + + +C C+ G+ G CE C G Y N G C C C+ GS+ NC+ G C+C P G+ CD+C Y
Sbjct: 429 CDPDKHTGACAEETGKCE-CLPRFVGEDCDQCASGYYDAPK----CKPCEC-----NVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADG-YTNVTAG--CVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGY 556 HSP 17 Score: 59.6918 bits (143), Expect = 5.830e-8 Identity = 43/142 (30.28%), Postives = 61/142 (42.96%), Query Frame = 2
Query: 4661 GDCQNCMGNREGNKCQLCREGFYKNDK-NYDQCEPCQC----PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNC-NEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSC 5068
G C+NC N EG C C G+++ + +++ +PC+ P H T KC+C + G+ C+ C G+Y P CK C+CN G+ C E G C C G G C+ CA Y C C
Sbjct: 389 GVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGACAEETG-------KCECLPRFVGEDCDQCASGYYDAP----KCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVEC 519 HSP 18 Score: 58.9214 bits (141), Expect = 9.559e-8 Identity = 61/229 (26.64%), Postives = 86/229 (37.55%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFN----------LDSANPEGCEP---CYCFG 1657
+C CK G+ CETC + A C EC C+ +E N + +T Q C+ G++C+KC G++ C C C +GT G + DQ G C+CK+G+ G CD C I + YPN C C C G C C + + C C G++N L A + C+ CYC G
Sbjct: 489 GQCPCKAGFGGTFCETCADGYTNV----TAGCVECVCDATGSE-HGNCSASTGQCE---------------CKPAYAGLSCDKCQVGYFGD---------DCKFCNCDPMGTEGG-------VCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPN--CKACACDGAGITSPECDATSGQCPCNGNF-TGRTCDKCAAGFYNYPDCRGCECLLSGAKGQTCDSNGQCYCKG 678 HSP 19 Score: 58.151 bits (139), Expect = 1.594e-7 Identity = 44/139 (31.65%), Postives = 65/139 (46.76%), Query Frame = 2
Query: 4616 KCNCNG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQC 5026
+CN NG VC G C C G C+ C +G+ C C C + NC S+ +C+C+ Y+G SC+ CQ G++G+ CK C+C+ G++ C++ G C C G G KCD+C
Sbjct: 473 ECNVNGTIGDVCLPEDGQCP-CKAGFGGTFCETCADGYTNVTAG---CVECVCDATGSEHG-NC----SASTGQCECKPAYAGLSCDKCQVGYFGD-----DCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRC 597 HSP 20 Score: 55.8398 bits (133), Expect = 7.325e-7 Identity = 68/261 (26.05%), Postives = 106/261 (40.61%), Query Frame = 2
Query: 914 YYSLRNIEIGGKCI---CNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCP-----LFNQRPWKPVA-VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHG---LGTIGSCVSNGEDIGDQKPGDC-ICKKGYEGVNCDLCSIGY--ERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
Y+ +++ G C+ CN H+ CD + GI +C+HNT G +CE C N P+ +A C N + C ++ LQ NC+ G C++C GF+ + C+PCQC+G L SC N GDC +C++ +G +C+ C+ + + C C CS GS C CK++V D C CK ++ +GC+ C+C
Sbjct: 1826 YHRVQSGSFLGACVPCECNGHSATCDP--DTGICT---DCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQC---------------NCKPGYTGDRCDRCASGFFGHPQISGES---CSPCQCNGNNNLTDSRSCHPNS--------GDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECSQCGSQYCD--NKSGGCECKINVEGDS-CDRCKPDHWGFSKC--QGCQGCHC 2050 HSP 21 Score: 55.0694 bits (131), Expect = 1.408e-6 Identity = 44/151 (29.14%), Postives = 72/151 (47.68%), Query Frame = 2
Query: 4613 KKCNCN--GYSK---VCDSVT-GDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
++CNCN G ++ CD V+ G+ +C + G C C+ F+ ++ D C C C N +++ +C C+ G+ C+ C GFY N + C+SC C+ G+ C+ G C+C P V G++CDQ N+
Sbjct: 699 EECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTG---QCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENH 846
BLAST of Laminin subunit alpha-2 vs. Ensembl Celegans
Match: epi-1 (Laminin-like protein epi-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21313]) HSP 1 Score: 293.893 bits (751), Expect = 6.603e-79 Identity = 184/561 (32.80%), Postives = 273/561 (48.66%), Query Frame = 2
Query: 95 RIFIIFILLIVHKAHSQDDEPEILSVNVDGLLMENAKITSSATCG--TGGP--EIFCRLVDEIAIQRL-----------------INP---DSHVLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSA 1687
+ + + L+ +SQ P ++++ IT+++TCG G P EI+C L L NP H C C+ NE HP D +N WW SP +++GL++ VN+TIDL+Q + + Y+ ++M +SPRPG+W+LERS D G+ Y PW FAE C F + + P S DD + C T + + L + E++I ++ R S + ++ + + L NF A +R R L TL LM + + ++ RY+Y+++ I IGG+C+CN HA CD + C C+HNTCG CE CCP F Q+ W+ CE CNC +SNEC Y+ V + S++ G EGGGVC NC+ENTEG+NC KC G++RP V E PC C C G+C ++ G C C + G +CD C+ GY P +C PC C+++G++ C CK C+TC +GY N+ + GC C C ++ + SA
Sbjct: 12 KALFLIVTLLAQFTYSQVLTPSQITIS------HRKPITATSTCGEIQGQPVTEIYCSLTGSTQYTPLNSYSYQDDEQQKSWSQYENPMVRGGHG-CGHCNAGNENS-HPAA-NMVDGNNSWWMSPPLSRGLQHNEVNITIDLEQEFHVAYVWIQMANSPRPGSWVLERSTDHGKTYQPWFNFAENAAECMRRFGME--------SLSPIS-EDDSVTCRTDMASLQPLENAEMVIRILEHRPS--SRQF----ATSEALQNFTRATNVRLRLLGTRTLQGHLMDMNEWRDPTVTRRYFYAIKEIMIGGRCVCNGHAVTCDILEPQRPKSLLCRCEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGACA--------EETGKCECLPRFVGEDCDQCASGYYDAP--KCKPCECNVNGTIGDVCLPEDGQCPCKAGFGG-TFCETCADGYTNVTA----GCVECVCDATGSEHGNCSA 533 HSP 2 Score: 285.419 bits (729), Expect = 2.771e-76 Identity = 225/745 (30.20%), Postives = 324/745 (43.49%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGF--YRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQC-SSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPD-KAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHL--ALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCH-KCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNN-----QESCDPVTGECR-CSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGL--YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKC 3184
+C CK G CE C P + P C +C CN +C E G C C + EG C++C+ Y P L C C CH G+ G G + D + G C+C++ G CD C G+ +P+ C C C+ G+ C CK +V C+ CK G F+L + NP GC C+CFG+T+ C SS+ + D+ + T G + K + Y + E S P Y+ P ++ TSYG + + + K D+ DV + + + + S + + Q + K LT + K T+EE + L + + +KA Y+ + K+ L L E + ++ + +E+C CP + G SC+ C SGY RV + + G C C C HS TCD G C +C HNT G HCE C G+YG+A + + C C CP S E + C +G CDRC G++G+P+ SC C+CN N SC P +G+C C NT G C+ C FYGDA+T K+C C+C G S+ CD +G C+C+ N CD C + K + C C+C G + CN +GQC C G RCE C GY+NY C KC C ++ CD+ G+C C CD+C
Sbjct: 1432 GQCKCKPGVIGRRCERCAPGYYNFPE-----CIKCQCN-AGQQCD------------------ERTGQCF-CPPHVEGQTCDRCVSNAFGYDP-------LIGCQKCGCHPQGSEG-----GNLVCDPESGQCLCRESMGGRQCDRCLAGFYGFPH--CYGCSCNRAGTTEEICDATNAQCKCKENVYGG-RCEACKAGTFDLSAENPLGCVNCFCFGVTDSCRSSMYPVTIMSVDMSSFLTTDDNG--MVDNKDDTVIYTSEETS--------PNSVYFNVPIEK-KDYTTSYGLKLTFKLSTVPRGGRKSMNADA-DVRLTGANMTIEYWASEQPTNPEEQFTVKCKLVPENF--LTAEGKTVTREELMKVLHSLQNITLKASYFDHPKTSTLYEFGLEISEPNGVDSVIKASSVEQCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECSQCG--SQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHC---GTAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQC 2117 HSP 3 Score: 241.891 bits (616), Expect = 4.150e-63 Identity = 167/515 (32.43%), Postives = 237/515 (46.02%), Query Frame = 2
Query: 2339 CEACACYNHSNTCD-----SLN-------------GECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQ---ESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHAL-SADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGL---------------DVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWD--FNETIGCKKCNCYINGSYS--NICNKISGICACKTAFFGSKCDRCNEGYYGFPN-----CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKC 3742
CEAC C+ SN C+ LN G C+NC NT G +C C+ GY+ + + + + PC D + + + E C +CL G CD+C GYY PK CK C+CN N + C P G+C C GT C+ C G+ +T C +C CD+ GS NC TG+C+C+ + CD C G + D C CNC+ G + C+ T+GQC C E GD+C++C ++ YPNCK C C + S +CD +G+C C N R CDKC Y N DC C C G+ CD G C C N C +C ++ D GC K P G++C+ C + G +C++C WD ++ GC+ C+C +NG+ S N C+ +G C CK G +CD+C +G+Y + C+ C C G E CD +GQC+C+ V G +C
Sbjct: 354 CEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYF---RPEGVTWNEPQPCKVCDCDPDKHTGACAEETGKC-ECLPRFVGEDCDQCASGYYDAPK----CKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTN--VTAGCVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGYFGDD--CKFCNCDPMG--TEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFY--NYPDCRGCECLLSGAKGQTCD-SNGQCYCKGNFEGERCDRCKPNFYNFPICEECNCNPSGVTRDFQGCDKVSP---GELCS------CRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAE---CDITSGQCKCRPRVTGLRC 839 HSP 4 Score: 240.736 bits (613), Expect = 9.854e-63 Identity = 188/633 (29.70%), Postives = 283/633 (44.71%), Query Frame = 2
Query: 3221 VPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSN--ICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLN-ANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKN---YDQCEPCQCPSAHY-NFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQG----SQSLNCNEFGICQCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
+ C+C +GS + +C+ G CKC V R C +C GY+ + C KC C GQ C+ G C CPP+ G C++C +++ ++ IGC+KC C+ GS +C+ SG C C+ + G +CDRC G+YGFP+C CSC GT + E+ CD QC+CK+NV G +C+ C+ GTF GC +CFCFGV++ C + + + + + + N + KD + I+ + P + + + + Y L + LS R +++ D +V + N + Y +E N + + + + F+ E V L + + +L N+T LK + L + + + I + ++VE+C C A G CQ SGY++ +SGS L C CNG+S CD TG C +C N G+ C+ C EG Y N N YD C C CP A NFA++C E L+C C+ GY+G C+ C GF+G+P I G C C CN S+S + N C G C+ CA + V K C SC
Sbjct: 1413 LSCDCVAQGSESFQCEQYGGQCKCKPGVIGRRCERCAPGYY--NFPECIKCQ-CNAGQQCDERTGQCFCPPHVEGQTCDRCVSNAFGYDPLIGCQKCGCHPQGSEGGNLVCDPESGQCLCRESMGGRQCDRCLAGFYGFPHCYGCSCNRAGT-TEEI--CDATNAQCKCKENVYGGRCEACKAGTFDLSAENPLGCVNCFCFGVTDSCRSSMYPVTIMSVDMSSFLTTDDNGMVDNKDDTVIYTSEETSPNSVYFNVPIEKKDYTTSYGLKLTFKLSTVPRGGRKSMNAD-------------ADVRLTGAN--MTIEYWA--------------SEQPTNPEEQFTVKCKLVPENFLTAEGKTVTREELMK--VLHSLQNIT-LKASYFDHPKTSTLYEFGLEISEPNGVDSVI--KASSVEQCQCPAPYTGPSCQLCASGYHR--------VQSGSF------LGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYD-CMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSC 1990 HSP 5 Score: 221.09 bits (562), Expect = 9.058e-57 Identity = 168/528 (31.82%), Postives = 243/528 (46.02%), Query Frame = 2
Query: 2348 CACYNHSNTCDSLNGE------CQNCTHNTAGAHCEDCASGY----YGDAKSKNLNSCKKCPC----------PRLDYQMTELCSSYSSENY-VCHKCLHNTSGVHCDRCIEGYYGNPK-----KKISCKRCKCNNNQE--SCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGL-DVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNI--CNKISG--ICACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
C C H+ TCD L + C+ C HNT G CE C G+ + A + N +C+ C C +D + S + E VC C NT GV+C++C GY+ P+ + CK C C+ ++ +C TG+C C +G +C C G+Y DA CK C+C+ G+ + C G+C C+ F C++C G C+ C C+ G S C+ ++GQC C G C+KC GYF +CK C C P CD G+C C CD+C+ Y +C C C G G +P+CD +G C C N R C KC G++ D GC+ GQ C++ G C C N G C++C + ++F C++CNC +G + C+K+S +C+C+ G CD+C ++ C+ C C NGT S L CD TGQC CK+N G +C C G + + + GC SC C
Sbjct: 298 CVCNGHAVTCDILEPQRPKSLLCR-CEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYF-RPEGVTWNEPQPCKVCDCDPDKHTGACAEETGKCECLPRFVGEDCDQCASGYY-DA--PKCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVECVCDATG--SEHGNCSASTGQCECKPAYAGLSCDKCQVGYFG-DDCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFY--GYPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYNYPDCRGCECLLSGAKGQTCDSNGQCYCKGNFEGERCDRCKPNFYNFPI---CEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISG-LNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHC 811 HSP 6 Score: 180.259 bits (456), Expect = 1.973e-44 Identity = 138/434 (31.80%), Postives = 205/434 (47.24%), Query Frame = 2
Query: 2246 KCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHS---NTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCN---NNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD---CD-LNNGK-CKCGSNVINRICDKCENLHY---WNNRTDCVPCNC---GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV---TGCKKCPPCYNGQVCNNE-----GICVCPPNTIGNLCNKCSEDSW 3472
KC C P F G C+ C SGY +C+ C C + + C +G+C C G CE CA GY + C +C C + CS+ + + C +C +G+ CD+C GY+G+ CK C C+ CD TG+C C G +C C FYG +CK C CD G TS CD +G+C C NF R CD C +G Y C C C + G + + C+ ++GQC+C N G+RC++C ++N+P C++C C+P ++ D CD ++ G+ C C +V RICD+C+ + +++ C C+C G S CD++TG C C +N R C +C G++ L+ GC+ C C G E G C C P G C++ E+ +
Sbjct: 444 KCECLPRFVGEDCDQCASGYYDAP------KCKPCECNVNGTIGDVCLPEDGQCP-CKAGFGGTFCETCADGY-----TNVTAGCVECVCDATGSEHGN-CSASTGQ---C-ECKPAYAGLSCDKCQVGYFGD-----DCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYG---YPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFY-NYPDCRGCECLLSG--AKGQTCD-SNGQCYCKGNFEGERCDRCKPNFYNFPICEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESC-HCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENHY 847 HSP 7 Score: 163.696 bits (413), Expect = 1.821e-39 Identity = 129/418 (30.86%), Postives = 186/418 (44.50%), Query Frame = 2
Query: 2624 YYGNPKKKISCKRCKCNNNQESCD------PVTGECRCSYNTMGTECQLCKHGFYG---DALTK----SCKKCDCDSLGSTSE-------NCDKLT----------GKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCR 3754
Y+ K+ + RC CN + +CD P + CRC +NT G C+ C GF A T +C+ C+C G ++E + +K + G C +CR N C+ C+ G + + + C C+C+ + C +G+C CL G+ C++C GY++ P CK C C+ + D C +G+C C + C+ C + Y N CV C C GS + C TG C+C A C KC VGYFG D C P G VC+ G C+C G+ C++C + + CK C C G S C+ SG C C F G CD+C G+Y +P+C+ C C +G K CD GQC CK N G +C C+
Sbjct: 285 YFYAIKEIMIGGRCVCNGHAVTCDILEPQRPKSLLCRCEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNC--FGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGA---CAEETGKCECLPRFVGEDCDQCASGYYDAPKCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCAD-GYTNVTAGCVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGYFGDDCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPN---CKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYNYPDCRGCECLLSGAKGQ---TCDS-NGQCYCKGNFEGERCDRCK 689 HSP 8 Score: 148.288 bits (373), Expect = 8.436e-35 Identity = 118/387 (30.49%), Postives = 169/387 (43.67%), Query Frame = 2
Query: 2231 MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHK------CLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNN---NQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTS-GQ-CFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPHALSAD---CDLNNGKCKCGSNVINRICDKCENLHYW---NNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSK 3322
+P +C C GF G CE+C GY V + C+A H N C + G+C+ C AG C+ C GY+GD CK C C + +E VC + C +G CDRC +YG P +CK C C+ CD +G+C C+ N G C C GFY C+ C+C G+ + CD G+C C+ NF +CD C Y C CNC G + C+ S G+ C C ++V G C++C +++ C+ C C+ + + CDL G+C C N R CD+C + Y N+ C C+C G+L +CD+ +G CKC V C +
Sbjct: 485 LPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVECVCDATGS-EHGN-CSASTGQCE-CKPAYAGLSCDKCQVGYFGD-------DCKFCNCDPM-----------GTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYP----NCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYN---YPDCRGCECLLSGAKGQTCDS-NGQCYCKGNFEGERCDRCKPNFY-NFPICEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQ 841 HSP 9 Score: 145.206 bits (365), Expect = 8.466e-34 Identity = 102/338 (30.18%), Postives = 155/338 (45.86%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPKKKI--SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGST--SENCDKLTG----KCDCRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR-TDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCN 3508
+C +G C C GY+ +C C+CN + +CDP TG C C +NT G C+ C G YG+A S C C C + +++CD +C+C+ + +CD C SG + + + C PC C ++ + C+ SG C+ C +N +G CE C ++ NC C CS S CD +G C+C NV CD+C+ H+ ++ C C+CG + N +C++E G C C C C+ GY+ GC KC G VC+ G C C G+ C++C S+ T GC++C+
Sbjct: 1798 QCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECS-QCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCGTAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQCLP-SYLRIPTYGCRRCD 2133 HSP 10 Score: 136.732 bits (343), Expect = 2.828e-31 Identity = 108/369 (29.27%), Postives = 154/369 (41.73%), Query Frame = 2
Query: 2708 ECRCSYNTMGTECQLCKHGFY---GDALTKSCKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKDK-----KCIPCNCEIQGRISSEKFCNTTSG----QCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY----NGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGY--YGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
+C+C G CQLC G++ + +C C+C+ +T CD TG C DC N C+ C G Y C+ C C + K C+ + QC C GDRC++C G+F +P CSP C+C N NN TD + C +G+C C QN R C C ++G VT K C C Q C+N+ G C C N G+ C++C D W F++ GC+ C+C +++ CN +G C C+ G +C+ C GY YG C C C + + T CD TGQC C++ G++C C
Sbjct: 1796 QCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSAT---CDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSP-------------CQCNGN---------------NNLTD-----------SRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTA-KNCSSCECSQCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCG-TAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMG---TVCDVRTGQCHCQEGATGSRCDQC 2117 HSP 11 Score: 103.605 bits (257), Expect = 3.065e-21 Identity = 92/299 (30.77%), Postives = 123/299 (41.14%), Query Frame = 2
Query: 3002 QCFCLENVNGDRCEKCVEGYFNYPN------CKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWNNRT----DCVPCNCGRGSLN---PKCDM-ETGN---CKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY--------NGQVCN-NEGIC-VCPPNTIGNLCNKCSEDSWDFNETIGCKKCN-CYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
QC C G C+ C GY + C C C+ H SA CD + G C C N C+ C HY N DC+ C C N CD+ E G C C C +C G+FG + C PC + + C+ N G C +C NT G C C+ +W + + + K C+ C + S C+ SG C CK G CDRC ++GF CQ C GT + T+C+ GQC C+ G +C+ C G + E GC C C
Sbjct: 1796 QCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGH--SATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCA--AWFYGDAVTAKNCSSCECSQCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCGTAAFN-TQCNVENGQCTCRPGATGMRCEHCEHGYW---NYGEHGCDKCDC 2086 HSP 12 Score: 100.523 bits (249), Expect = 2.665e-20 Identity = 163/813 (20.05%), Postives = 311/813 (38.25%), Query Frame = 2
Query: 4481 ICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSCYS------GCTKDLLVRIDTLNETVINFKWKTLPSTLDLR----LNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQ----KMKDDAKEKYGE-----------IEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEM--PNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDA----ISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLN---------KSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDI----LAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIP-----------TGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRN---LEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELI----HGAAARSVE---PIRHVVRCVRHY-VTYYLPTSNAFSLEFYM 6712
+C T G HC++ + +Y + N + C C+ S+ CD+ +G C+ C N EG+ C C+ + K C+ C C +A +N N +E+ +C CR G +G CE+C+ G++ G C CDC S C+ G C C G G +CDQC P+Y+ + C+ C G +L + ID L + N T+ R N IN +M ++ NS + +I S Q Q + K ++ ++++ R + + +RS S + + + L+K A L T++ + L+++ K + E + + + ++ + K +A +N+ + + S K + + +L ++++L ++ + + + QKL+ I+++ +D+++ ++ V + + +++ +K + D +K V+ QL +++ L + + + A +A A+SN+T + +D A E F S N K E + + + KE EKL KQ + + +KL+ E + + IP +VE LK + E + + + L RN L KL V+ +++ +L SS K+ + + ++E + AR + I H S++ P R V R H +++Y T + +
Sbjct: 1960 LCEQNTDGRHCESCAAWFYGDAVTAKNCSS----------------CECSQCGSQYCDNKSGGCE-CKINVEGDSCDRCKPDHWGFSK-CQGCQGCHCGTAAFNTQCN---VENG---QCTCRPGATGMRCEHCEHGYWNYGEHG--CDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQCLPSYLRIPTYGCRRCDECVHHLIGDVDNLELEIDVLGTAIANISSATIVGARLARNKKEFNDINEITKMLNDEENSFGNVFGDAQDILTNSTQIQNKLVRTKTHSQNSVSSAKNITLNGTEFLQEVMKRAQRARQSVRSLAEIALAIGSSSKAVNVDPRLLKEAEETLMTLEAASADQYPEKAQTVPGKLEEIQKKIQEETEKLDKQKETFEAQK-------KRAEELAAYLNSAQQLLKESKSKADKSNNIAKMLQLTKVENLVAAITDDLERVEAAKGEFQKLNVAIGNITENLKDKREEMTHAVTTLNETRNDVAEALEAAKKRVRRD--EKSVDMQLVNAKAHELHLQATTLRQTFDNNKDNTDQAVEAANAFSNLTDTLKNAKAQIDNAYEALSAEPAFAESVQNARD-KPFPDETKEKIDALSKTVSQDLKETEKLKKQLEQLTELSEKLRKRKEAVKAGIPKYSKNTLDSIDEKVQEVEKLKAEIDANIEETRAKISEIAGKAEEITEKANSAMEGIRLARRNSVQLNKLAPVIVSKFEELKKLSSARSAKVDSVSDKVSQIKEMIAVARDAANRIKLGAHFEKGSSLDLNIPQR-VTRSAAHADISFYFRTEQEHGIPLFF 2735 HSP 13 Score: 70.0922 bits (170), Expect = 3.593e-11 Identity = 52/166 (31.33%), Postives = 72/166 (43.37%), Query Frame = 2
Query: 4613 KKCNCNGYSK---VCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSC---YSGCTKDL 5092
K CNC+ VCD TG C C G+KC C FY Y C+ C C A + C +C C ++G++C+ C GFY P C+ C+C G++ C+ G C C G +CD+C PN+ C+ C SG T+D
Sbjct: 562 KFCNCDPMGTEGGVCDQTTGQCL-CKEGFAGDKCDRCDIAFY----GYPNCKACACDGAGIT-SPECDATSG----QCPCNGNFTGRTCDKCAAGFYNYP----DCRGCECLLSGAKGQTCDSNGQCYCKGNFEGERCDRCKPNF-YNFPICEECNCNPSGVTRDF 712 HSP 14 Score: 67.0106 bits (162), Expect = 3.723e-10 Identity = 59/204 (28.92%), Postives = 87/204 (42.65%), Query Frame = 2
Query: 1004 SVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYER---YPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYF 1606
S +C CK N G C+ C P F P +CEECNCN + + ++ G +C +C+++ G C++C F W++ C C C+ GTI +G + D K G C+CKK +G CD C+ G+ R Y C C C I G++R C C+ V + +N YF
Sbjct: 670 SNGQCYCKGNFEGERCDRCKPNFYNFP-----ICEECNCNP-----------SGVTRDFQGCDKVSPGELC-SCRKHVTGRICDQCKPTF---WDLQYHHEDGCRSCDCNVNGTI-----SGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENHYF 848 HSP 15 Score: 65.4698 bits (158), Expect = 9.518e-10 Identity = 65/208 (31.25%), Postives = 91/208 (43.75%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIP-CKSCDCNQQGSQS-LNCNEF--GICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCY 5071
+C CN G C +G+Y N P+ + +G + + CDS G C C GN EG +C C+ FY N+ CE C C PS Q C ++ S EL C CR +G+ C+ C+ F+ C+SCDCN G+ S LN + G C C G +CDQCA + + C+SC+
Sbjct: 628 QCPCNGNFTGRTCDKCAAGFY----NYPDCRGCECLLSGAK-------------GQTCDS-NGQCY-CKGNFEGERCDRCKPNFY----NFPICEECNCNPSGVTRDFQGCDKV-SPGEL-CSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCH 810 HSP 16 Score: 65.0846 bits (157), Expect = 1.376e-9 Identity = 44/141 (31.21%), Postives = 64/141 (45.39%), Query Frame = 2
Query: 4619 CNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
C+ + ++ C TG C+ C+ G C C G+Y K C+PC+C N + + +C C+ G+ G CE C G Y N G C C C+ GS+ NC+ G C+C P G+ CD+C Y
Sbjct: 429 CDPDKHTGACAEETGKCE-CLPRFVGEDCDQCASGYYDAPK----CKPCEC-----NVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADG-YTNVTAG--CVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGY 556 HSP 17 Score: 59.3066 bits (142), Expect = 6.233e-8 Identity = 43/142 (30.28%), Postives = 61/142 (42.96%), Query Frame = 2
Query: 4661 GDCQNCMGNREGNKCQLCREGFYKNDK-NYDQCEPCQC----PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNC-NEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSC 5068
G C+NC N EG C C G+++ + +++ +PC+ P H T KC+C + G+ C+ C G+Y P CK C+CN G+ C E G C C G G C+ CA Y C C
Sbjct: 389 GVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGACAEETG-------KCECLPRFVGEDCDQCASGYYDAP----KCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVEC 519 HSP 18 Score: 58.9214 bits (141), Expect = 9.966e-8 Identity = 61/229 (26.64%), Postives = 86/229 (37.55%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFN----------LDSANPEGCEP---CYCFG 1657
+C CK G+ CETC + A C EC C+ +E N + +T Q C+ G++C+KC G++ C C C +GT G + DQ G C+CK+G+ G CD C I + YPN C C C G C C + + C C G++N L A + C+ CYC G
Sbjct: 489 GQCPCKAGFGGTFCETCADGYTNV----TAGCVECVCDATGSE-HGNCSASTGQCE---------------CKPAYAGLSCDKCQVGYFGD---------DCKFCNCDPMGTEGG-------VCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPN--CKACACDGAGITSPECDATSGQCPCNGNF-TGRTCDKCAAGFYNYPDCRGCECLLSGAKGQTCDSNGQCYCKG 678 HSP 19 Score: 58.151 bits (139), Expect = 1.541e-7 Identity = 44/139 (31.65%), Postives = 65/139 (46.76%), Query Frame = 2
Query: 4616 KCNCNG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQC 5026
+CN NG VC G C C G C+ C +G+ C C C + NC S+ +C+C+ Y+G SC+ CQ G++G+ CK C+C+ G++ C++ G C C G G KCD+C
Sbjct: 473 ECNVNGTIGDVCLPEDGQCP-CKAGFGGTFCETCADGYTNVTAG---CVECVCDATGSEHG-NC----SASTGQCECKPAYAGLSCDKCQVGYFGD-----DCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRC 597 HSP 20 Score: 55.8398 bits (133), Expect = 7.573e-7 Identity = 68/261 (26.05%), Postives = 106/261 (40.61%), Query Frame = 2
Query: 914 YYSLRNIEIGGKCI---CNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCP-----LFNQRPWKPVA-VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHG---LGTIGSCVSNGEDIGDQKPGDC-ICKKGYEGVNCDLCSIGY--ERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
Y+ +++ G C+ CN H+ CD + GI +C+HNT G +CE C N P+ +A C N + C ++ LQ NC+ G C++C GF+ + C+PCQC+G L SC N GDC +C++ +G +C+ C+ + + C C CS GS C CK++V D C CK ++ +GC+ C+C
Sbjct: 1815 YHRVQSGSFLGACVPCECNGHSATCDP--DTGICT---DCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQC---------------NCKPGYTGDRCDRCASGFFGHPQISGES---CSPCQCNGNNNLTDSRSCHPNS--------GDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECSQCGSQYCD--NKSGGCECKINVEGDS-CDRCKPDHWGFSKC--QGCQGCHC 2039 HSP 21 Score: 55.0694 bits (131), Expect = 1.396e-6 Identity = 44/151 (29.14%), Postives = 72/151 (47.68%), Query Frame = 2
Query: 4613 KKCNCN--GYSK---VCDSVT-GDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
++CNCN G ++ CD V+ G+ +C + G C C+ F+ ++ D C C C N +++ +C C+ G+ C+ C GFY N + C+SC C+ G+ C+ G C+C P V G++CDQ N+
Sbjct: 699 EECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTG---QCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENH 846
BLAST of Laminin subunit alpha-2 vs. Ensembl Celegans
Match: epi-1 (Laminin-like protein epi-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21313]) HSP 1 Score: 293.893 bits (751), Expect = 6.934e-79 Identity = 184/561 (32.80%), Postives = 273/561 (48.66%), Query Frame = 2
Query: 95 RIFIIFILLIVHKAHSQDDEPEILSVNVDGLLMENAKITSSATCG--TGGP--EIFCRLVDEIAIQRL-----------------INP---DSHVLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSA 1687
+ + + L+ +SQ P ++++ IT+++TCG G P EI+C L L NP H C C+ NE HP D +N WW SP +++GL++ VN+TIDL+Q + + Y+ ++M +SPRPG+W+LERS D G+ Y PW FAE C F + + P S DD + C T + + L + E++I ++ R S + ++ + + L NF A +R R L TL LM + + ++ RY+Y+++ I IGG+C+CN HA CD + C C+HNTCG CE CCP F Q+ W+ CE CNC +SNEC Y+ V + S++ G EGGGVC NC+ENTEG+NC KC G++RP V E PC C C G+C ++ G C C + G +CD C+ GY P +C PC C+++G++ C CK C+TC +GY N+ + GC C C ++ + SA
Sbjct: 12 KALFLIVTLLAQFTYSQVLTPSQITIS------HRKPITATSTCGEIQGQPVTEIYCSLTGSTQYTPLNSYSYQDDEQQKSWSQYENPMVRGGHG-CGHCNAGNENS-HPAA-NMVDGNNSWWMSPPLSRGLQHNEVNITIDLEQEFHVAYVWIQMANSPRPGSWVLERSTDHGKTYQPWFNFAENAAECMRRFGME--------SLSPIS-EDDSVTCRTDMASLQPLENAEMVIRILEHRPS--SRQF----ATSEALQNFTRATNVRLRLLGTRTLQGHLMDMNEWRDPTVTRRYFYAIKEIMIGGRCVCNGHAVTCDILEPQRPKSLLCRCEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGACA--------EETGKCECLPRFVGEDCDQCASGYYDAP--KCKPCECNVNGTIGDVCLPEDGQCPCKAGFGG-TFCETCADGYTNVTA----GCVECVCDATGSEHGNCSA 533 HSP 2 Score: 285.034 bits (728), Expect = 3.139e-76 Identity = 225/745 (30.20%), Postives = 324/745 (43.49%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGF--YRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQC-SSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPD-KAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHL--ALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCH-KCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNN-----QESCDPVTGECR-CSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGL--YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKC 3184
+C CK G CE C P + P C +C CN +C E G C C + EG C++C+ Y P L C C CH G+ G G + D + G C+C++ G CD C G+ +P+ C C C+ G+ C CK +V C+ CK G F+L + NP GC C+CFG+T+ C SS+ + D+ + T G + K + Y + E S P Y+ P ++ TSYG + + + K D+ DV + + + + S + + Q + K LT + K T+EE + L + + +KA Y+ + K+ L L E + ++ + +E+C CP + G SC+ C SGY RV + + G C C C HS TCD G C +C HNT G HCE C G+YG+A + + C C CP S E + C +G CDRC G++G+P+ SC C+CN N SC P +G+C C NT G C+ C FYGDA+T K+C C+C G S+ CD +G C+C+ N CD C + K + C C+C G + CN +GQC C G RCE C GY+NY C KC C ++ CD+ G+C C CD+C
Sbjct: 1423 GQCKCKPGVIGRRCERCAPGYYNFPE-----CIKCQCN-AGQQCD------------------ERTGQCF-CPPHVEGQTCDRCVSNAFGYDP-------LIGCQKCGCHPQGSEG-----GNLVCDPESGQCLCRESMGGRQCDRCLAGFYGFPH--CYGCSCNRAGTTEEICDATNAQCKCKENVYGG-RCEACKAGTFDLSAENPLGCVNCFCFGVTDSCRSSMYPVTIMSVDMSSFLTTDDNG--MVDNKDDTVIYTSEETS--------PNSVYFNVPIEK-KDYTTSYGLKLTFKLSTVPRGGRKSMNADA-DVRLTGANMTIEYWASEQPTNPEEQFTVKCKLVPENF--LTAEGKTVTREELMKVLHSLQNITLKASYFDHPKTSTLYEFGLEISEPNGVDSVIKASSVEQCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECSQCG--SQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHC---GTAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQC 2108 HSP 3 Score: 241.891 bits (616), Expect = 4.471e-63 Identity = 167/515 (32.43%), Postives = 237/515 (46.02%), Query Frame = 2
Query: 2339 CEACACYNHSNTCD-----SLN-------------GECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQ---ESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHAL-SADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGL---------------DVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWD--FNETIGCKKCNCYINGSYS--NICNKISGICACKTAFFGSKCDRCNEGYYGFPN-----CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKC 3742
CEAC C+ SN C+ LN G C+NC NT G +C C+ GY+ + + + + PC D + + + E C +CL G CD+C GYY PK CK C+CN N + C P G+C C GT C+ C G+ +T C +C CD+ GS NC TG+C+C+ + CD C G + D C CNC+ G + C+ T+GQC C E GD+C++C ++ YPNCK C C + S +CD +G+C C N R CDKC Y N DC C C G+ CD G C C N C +C ++ D GC K P G++C+ C + G +C++C WD ++ GC+ C+C +NG+ S N C+ +G C CK G +CD+C +G+Y + C+ C C G E CD +GQC+C+ V G +C
Sbjct: 354 CEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYF---RPEGVTWNEPQPCKVCDCDPDKHTGACAEETGKC-ECLPRFVGEDCDQCASGYYDAPK----CKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTN--VTAGCVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGYFGDD--CKFCNCDPMG--TEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFY--NYPDCRGCECLLSGAKGQTCD-SNGQCYCKGNFEGERCDRCKPNFYNFPICEECNCNPSGVTRDFQGCDKVSP---GELCS------CRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAE---CDITSGQCKCRPRVTGLRC 839 HSP 4 Score: 240.35 bits (612), Expect = 1.044e-62 Identity = 188/633 (29.70%), Postives = 283/633 (44.71%), Query Frame = 2
Query: 3221 VPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSN--ICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLN-ANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKN---YDQCEPCQCPSAHY-NFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQG----SQSLNCNEFGICQCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
+ C+C +GS + +C+ G CKC V R C +C GY+ + C KC C GQ C+ G C CPP+ G C++C +++ ++ IGC+KC C+ GS +C+ SG C C+ + G +CDRC G+YGFP+C CSC GT + E+ CD QC+CK+NV G +C+ C+ GTF GC +CFCFGV++ C + + + + + + N + KD + I+ + P + + + + Y L + LS R +++ D +V + N + Y +E N + + + + F+ E V L + + +L N+T LK + L + + + I + ++VE+C C A G CQ SGY++ +SGS L C CNG+S CD TG C +C N G+ C+ C EG Y N N YD C C CP A NFA++C E L+C C+ GY+G C+ C GF+G+P I G C C CN S+S + N C G C+ CA + V K C SC
Sbjct: 1404 LSCDCVAQGSESFQCEQYGGQCKCKPGVIGRRCERCAPGYY--NFPECIKCQ-CNAGQQCDERTGQCFCPPHVEGQTCDRCVSNAFGYDPLIGCQKCGCHPQGSEGGNLVCDPESGQCLCRESMGGRQCDRCLAGFYGFPHCYGCSCNRAGT-TEEI--CDATNAQCKCKENVYGGRCEACKAGTFDLSAENPLGCVNCFCFGVTDSCRSSMYPVTIMSVDMSSFLTTDDNGMVDNKDDTVIYTSEETSPNSVYFNVPIEKKDYTTSYGLKLTFKLSTVPRGGRKSMNAD-------------ADVRLTGAN--MTIEYWA--------------SEQPTNPEEQFTVKCKLVPENFLTAEGKTVTREELMK--VLHSLQNIT-LKASYFDHPKTSTLYEFGLEISEPNGVDSVI--KASSVEQCQCPAPYTGPSCQLCASGYHR--------VQSGSF------LGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYD-CMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSC 1981 HSP 5 Score: 220.705 bits (561), Expect = 9.596e-57 Identity = 168/528 (31.82%), Postives = 243/528 (46.02%), Query Frame = 2
Query: 2348 CACYNHSNTCDSLNGE------CQNCTHNTAGAHCEDCASGY----YGDAKSKNLNSCKKCPC----------PRLDYQMTELCSSYSSENY-VCHKCLHNTSGVHCDRCIEGYYGNPK-----KKISCKRCKCNNNQE--SCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGL-DVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNI--CNKISG--ICACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
C C H+ TCD L + C+ C HNT G CE C G+ + A + N +C+ C C +D + S + E VC C NT GV+C++C GY+ P+ + CK C C+ ++ +C TG+C C +G +C C G+Y DA CK C+C+ G+ + C G+C C+ F C++C G C+ C C+ G S C+ ++GQC C G C+KC GYF +CK C C P CD G+C C CD+C+ Y +C C C G G +P+CD +G C C N R C KC G++ D GC+ GQ C++ G C C N G C++C + ++F C++CNC +G + C+K+S +C+C+ G CD+C ++ C+ C C NGT S L CD TGQC CK+N G +C C G + + + GC SC C
Sbjct: 298 CVCNGHAVTCDILEPQRPKSLLCR-CEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNCFGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYF-RPEGVTWNEPQPCKVCDCDPDKHTGACAEETGKCECLPRFVGEDCDQCASGYY-DA--PKCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVECVCDATG--SEHGNCSASTGQCECKPAYAGLSCDKCQVGYFG-DDCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFY--GYPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYNYPDCRGCECLLSGAKGQTCDSNGQCYCKGNFEGERCDRCKPNFYNFPI---CEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISG-LNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHC 811 HSP 6 Score: 180.259 bits (456), Expect = 1.972e-44 Identity = 138/434 (31.80%), Postives = 205/434 (47.24%), Query Frame = 2
Query: 2246 KCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHS---NTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCN---NNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD---CD-LNNGK-CKCGSNVINRICDKCENLHY---WNNRTDCVPCNC---GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV---TGCKKCPPCYNGQVCNNE-----GICVCPPNTIGNLCNKCSEDSW 3472
KC C P F G C+ C SGY +C+ C C + + C +G+C C G CE CA GY + C +C C + CS+ + + C +C +G+ CD+C GY+G+ CK C C+ CD TG+C C G +C C FYG +CK C CD G TS CD +G+C C NF R CD C +G Y C C C + G + + C+ ++GQC+C N G+RC++C ++N+P C++C C+P ++ D CD ++ G+ C C +V RICD+C+ + +++ C C+C G S CD++TG C C +N R C +C G++ L+ GC+ C C G E G C C P G C++ E+ +
Sbjct: 444 KCECLPRFVGEDCDQCASGYYDAP------KCKPCECNVNGTIGDVCLPEDGQCP-CKAGFGGTFCETCADGY-----TNVTAGCVECVCDATGSEHGN-CSASTGQ---C-ECKPAYAGLSCDKCQVGYFGD-----DCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYG---YPNCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFY-NYPDCRGCECLLSG--AKGQTCD-SNGQCYCKGNFEGERCDRCKPNFYNFPICEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESC-HCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENHY 847 HSP 7 Score: 163.696 bits (413), Expect = 1.930e-39 Identity = 129/418 (30.86%), Postives = 186/418 (44.50%), Query Frame = 2
Query: 2624 YYGNPKKKISCKRCKCNNNQESCD------PVTGECRCSYNTMGTECQLCKHGFYG---DALTK----SCKKCDCDSLGSTSE-------NCDKLT----------GKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCR 3754
Y+ K+ + RC CN + +CD P + CRC +NT G C+ C GF A T +C+ C+C G ++E + +K + G C +CR N C+ C+ G + + + C C+C+ + C +G+C CL G+ C++C GY++ P CK C C+ + D C +G+C C + C+ C + Y N CV C C GS + C TG C+C A C KC VGYFG D C P G VC+ G C+C G+ C++C + + CK C C G S C+ SG C C F G CD+C G+Y +P+C+ C C +G K CD GQC CK N G +C C+
Sbjct: 285 YFYAIKEIMIGGRCVCNGHAVTCDILEPQRPKSLLCRCEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNC--FGRSNECEYDAEVDLNKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGA---CAEETGKCECLPRFVGEDCDQCASGYYDAPKCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCAD-GYTNVTAGCVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGYFGDDCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPN---CKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYNYPDCRGCECLLSGAKGQ---TCDS-NGQCYCKGNFEGERCDRCK 689 HSP 8 Score: 148.288 bits (373), Expect = 8.574e-35 Identity = 118/387 (30.49%), Postives = 169/387 (43.67%), Query Frame = 2
Query: 2231 MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHK------CLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNN---NQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTS-GQ-CFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPHALSAD---CDLNNGKCKCGSNVINRICDKCENLHYW---NNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSK 3322
+P +C C GF G CE+C GY V + C+A H N C + G+C+ C AG C+ C GY+GD CK C C + +E VC + C +G CDRC +YG P +CK C C+ CD +G+C C+ N G C C GFY C+ C+C G+ + CD G+C C+ NF +CD C Y C CNC G + C+ S G+ C C ++V G C++C +++ C+ C C+ + + CDL G+C C N R CD+C + Y N+ C C+C G+L +CD+ +G CKC V C +
Sbjct: 485 LPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVECVCDATGS-EHGN-CSASTGQCE-CKPAYAGLSCDKCQVGYFGD-------DCKFCNCDPM-----------GTEGGVCDQTTGQCLCKEGFAGDKCDRCDIAFYGYP----NCKACACDGAGITSPECDATSGQCPCNGNFTGRTCDKCAAGFYN---YPDCRGCECLLSGAKGQTCDS-NGQCYCKGNFEGERCDRCKPNFY-NFPICEECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQ 841 HSP 9 Score: 144.821 bits (364), Expect = 8.824e-34 Identity = 102/338 (30.18%), Postives = 155/338 (45.86%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPKKKI--SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGST--SENCDKLTG----KCDCRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR-TDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCN 3508
+C +G C C GY+ +C C+CN + +CDP TG C C +NT G C+ C G YG+A S C C C + +++CD +C+C+ + +CD C SG + + + C PC C ++ + C+ SG C+ C +N +G CE C ++ NC C CS S CD +G C+C NV CD+C+ H+ ++ C C+CG + N +C++E G C C C C+ GY+ GC KC G VC+ G C C G+ C++C S+ T GC++C+
Sbjct: 1789 QCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECS-QCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCGTAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQCLP-SYLRIPTYGCRRCD 2124 HSP 10 Score: 136.732 bits (343), Expect = 3.073e-31 Identity = 108/369 (29.27%), Postives = 154/369 (41.73%), Query Frame = 2
Query: 2708 ECRCSYNTMGTECQLCKHGFY---GDALTKSCKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKDK-----KCIPCNCEIQGRISSEKFCNTTSG----QCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY----NGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGY--YGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
+C+C G CQLC G++ + +C C+C+ +T CD TG C DC N C+ C G Y C+ C C + K C+ + QC C GDRC++C G+F +P CSP C+C N NN TD + C +G+C C QN R C C ++G VT K C C Q C+N+ G C C N G+ C++C D W F++ GC+ C+C +++ CN +G C C+ G +C+ C GY YG C C C + + T CD TGQC C++ G++C C
Sbjct: 1787 QCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGHSAT---CDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSP-------------CQCNGN---------------NNLTD-----------SRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTA-KNCSSCECSQCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCG-TAAFNTQCNVENGQCTCRPGATGMRCEHCEHGYWNYGEHGCDKCDCEADLSMG---TVCDVRTGQCHCQEGATGSRCDQC 2108 HSP 11 Score: 103.605 bits (257), Expect = 3.090e-21 Identity = 92/299 (30.77%), Postives = 123/299 (41.14%), Query Frame = 2
Query: 3002 QCFCLENVNGDRCEKCVEGYFNYPN------CKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWNNRT----DCVPCNCGRGSLN---PKCDM-ETGN---CKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY--------NGQVCN-NEGIC-VCPPNTIGNLCNKCSEDSWDFNETIGCKKCN-CYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
QC C G C+ C GY + C C C+ H SA CD + G C C N C+ C HY N DC+ C C N CD+ E G C C C +C G+FG + C PC + + C+ N G C +C NT G C C+ +W + + + K C+ C + S C+ SG C CK G CDRC ++GF CQ C GT + T+C+ GQC C+ G +C+ C G + E GC C C
Sbjct: 1787 QCQCPAPYTGPSCQLCASGYHRVQSGSFLGACVPCECNGH--SATCDPDTGICTDCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCA--AWFYGDAVTAKNCSSCECSQCGSQYCDNKSGGCECKINVEGDSCDRCKPDHWGFSKCQGCQGCHCGTAAFN-TQCNVENGQCTCRPGATGMRCEHCEHGYW---NYGEHGCDKCDC 2077 HSP 12 Score: 100.138 bits (248), Expect = 2.947e-20 Identity = 163/813 (20.05%), Postives = 311/813 (38.25%), Query Frame = 2
Query: 4481 ICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSCYS------GCTKDLLVRIDTLNETVINFKWKTLPSTLDLR----LNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQ----KMKDDAKEKYGE-----------IEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEM--PNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDA----ISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLN---------KSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDI----LAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIP-----------TGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRN---LEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELI----HGAAARSVE---PIRHVVRCVRHY-VTYYLPTSNAFSLEFYM 6712
+C T G HC++ + +Y + N + C C+ S+ CD+ +G C+ C N EG+ C C+ + K C+ C C +A +N N +E+ +C CR G +G CE+C+ G++ G C CDC S C+ G C C G G +CDQC P+Y+ + C+ C G +L + ID L + N T+ R N IN +M ++ NS + +I S Q Q + K ++ ++++ R + + +RS S + + + L+K A L T++ + L+++ K + E + + + ++ + K +A +N+ + + S K + + +L ++++L ++ + + + QKL+ I+++ +D+++ ++ V + + +++ +K + D +K V+ QL +++ L + + + A +A A+SN+T + +D A E F S N K E + + + KE EKL KQ + + +KL+ E + + IP +VE LK + E + + + L RN L KL V+ +++ +L SS K+ + + ++E + AR + I H S++ P R V R H +++Y T + +
Sbjct: 1951 LCEQNTDGRHCESCAAWFYGDAVTAKNCSS----------------CECSQCGSQYCDNKSGGCE-CKINVEGDSCDRCKPDHWGFSK-CQGCQGCHCGTAAFNTQCN---VENG---QCTCRPGATGMRCEHCEHGYWNYGEHG--CDKCDCEADLSMGTVCDVRTGQCHCQEGATGSRCDQCLPSYLRIPTYGCRRCDECVHHLIGDVDNLELEIDVLGTAIANISSATIVGARLARNKKEFNDINEITKMLNDEENSFGNVFGDAQDILTNSTQIQNKLVRTKTHSQNSVSSAKNITLNGTEFLQEVMKRAQRARQSVRSLAEIALAIGSSSKAVNVDPRLLKEAEETLMTLEAASADQYPEKAQTVPGKLEEIQKKIQEETEKLDKQKETFEAQK-------KRAEELAAYLNSAQQLLKESKSKADKSNNIAKMLQLTKVENLVAAITDDLERVEAAKGEFQKLNVAIGNITENLKDKREEMTHAVTTLNETRNDVAEALEAAKKRVRRD--EKSVDMQLVNAKAHELHLQATTLRQTFDNNKDNTDQAVEAANAFSNLTDTLKNAKAQIDNAYEALSAEPAFAESVQNARD-KPFPDETKEKIDALSKTVSQDLKETEKLKKQLEQLTELSEKLRKRKEAVKAGIPKYSKNTLDSIDEKVQEVEKLKAEIDANIEETRAKISEIAGKAEEITEKANSAMEGIRLARRNSVQLNKLAPVIVSKFEELKKLSSARSAKVDSVSDKVSQIKEMIAVARDAANRIKLGAHFEKGSSLDLNIPQR-VTRSAAHADISFYFRTEQEHGIPLFF 2726 HSP 13 Score: 70.0922 bits (170), Expect = 3.652e-11 Identity = 52/166 (31.33%), Postives = 72/166 (43.37%), Query Frame = 2
Query: 4613 KKCNCNGYSK---VCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSC---YSGCTKDL 5092
K CNC+ VCD TG C C G+KC C FY Y C+ C C A + C +C C ++G++C+ C GFY P C+ C+C G++ C+ G C C G +CD+C PN+ C+ C SG T+D
Sbjct: 562 KFCNCDPMGTEGGVCDQTTGQCL-CKEGFAGDKCDRCDIAFY----GYPNCKACACDGAGIT-SPECDATSG----QCPCNGNFTGRTCDKCAAGFYNYP----DCRGCECLLSGAKGQTCDSNGQCYCKGNFEGERCDRCKPNF-YNFPICEECNCNPSGVTRDF 712 HSP 14 Score: 66.6254 bits (161), Expect = 3.914e-10 Identity = 59/204 (28.92%), Postives = 87/204 (42.65%), Query Frame = 2
Query: 1004 SVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYER---YPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYF 1606
S +C CK N G C+ C P F P +CEECNCN + + ++ G +C +C+++ G C++C F W++ C C C+ GTI +G + D K G C+CKK +G CD C+ G+ R Y C C C I G++R C C+ V + +N YF
Sbjct: 670 SNGQCYCKGNFEGERCDRCKPNFYNFP-----ICEECNCNP-----------SGVTRDFQGCDKVSPGELC-SCRKHVTGRICDQCKPTF---WDLQYHHEDGCRSCDCNVNGTI-----SGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENHYF 848 HSP 15 Score: 65.4698 bits (158), Expect = 1.018e-9 Identity = 65/208 (31.25%), Postives = 91/208 (43.75%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIP-CKSCDCNQQGSQS-LNCNEF--GICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCY 5071
+C CN G C +G+Y N P+ + +G + + CDS G C C GN EG +C C+ FY N+ CE C C PS Q C ++ S EL C CR +G+ C+ C+ F+ C+SCDCN G+ S LN + G C C G +CDQCA + + C+SC+
Sbjct: 628 QCPCNGNFTGRTCDKCAAGFY----NYPDCRGCECLLSGAK-------------GQTCDS-NGQCY-CKGNFEGERCDRCKPNFY----NFPICEECNCNPSGVTRDFQGCDKV-SPGEL-CSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCH 810 HSP 16 Score: 65.0846 bits (157), Expect = 1.353e-9 Identity = 44/141 (31.21%), Postives = 64/141 (45.39%), Query Frame = 2
Query: 4619 CNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
C+ + ++ C TG C+ C+ G C C G+Y K C+PC+C N + + +C C+ G+ G CE C G Y N G C C C+ GS+ NC+ G C+C P G+ CD+C Y
Sbjct: 429 CDPDKHTGACAEETGKCE-CLPRFVGEDCDQCASGYYDAPK----CKPCEC-----NVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADG-YTNVTAG--CVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGY 556 HSP 17 Score: 59.3066 bits (142), Expect = 6.232e-8 Identity = 43/142 (30.28%), Postives = 61/142 (42.96%), Query Frame = 2
Query: 4661 GDCQNCMGNREGNKCQLCREGFYKNDK-NYDQCEPCQC----PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNC-NEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSC 5068
G C+NC N EG C C G+++ + +++ +PC+ P H T KC+C + G+ C+ C G+Y P CK C+CN G+ C E G C C G G C+ CA Y C C
Sbjct: 389 GVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGACAEETG-------KCECLPRFVGEDCDQCASGYYDAP----KCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVEC 519 HSP 18 Score: 58.5362 bits (140), Expect = 1.075e-7 Identity = 61/229 (26.64%), Postives = 86/229 (37.55%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFN----------LDSANPEGCEP---CYCFG 1657
+C CK G+ CETC + A C EC C+ +E N + +T Q C+ G++C+KC G++ C C C +GT G + DQ G C+CK+G+ G CD C I + YPN C C C G C C + + C C G++N L A + C+ CYC G
Sbjct: 489 GQCPCKAGFGGTFCETCADGYTNV----TAGCVECVCDATGSE-HGNCSASTGQCE---------------CKPAYAGLSCDKCQVGYFGD---------DCKFCNCDPMGTEGG-------VCDQTTGQCLCKEGFAGDKCDRCDIAFYGYPN--CKACACDGAGITSPECDATSGQCPCNGNF-TGRTCDKCAAGFYNYPDCRGCECLLSGAKGQTCDSNGQCYCKG 678 HSP 19 Score: 58.151 bits (139), Expect = 1.541e-7 Identity = 44/139 (31.65%), Postives = 65/139 (46.76%), Query Frame = 2
Query: 4616 KCNCNG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQC 5026
+CN NG VC G C C G C+ C +G+ C C C + NC S+ +C+C+ Y+G SC+ CQ G++G+ CK C+C+ G++ C++ G C C G G KCD+C
Sbjct: 473 ECNVNGTIGDVCLPEDGQCP-CKAGFGGTFCETCADGYTNVTAG---CVECVCDATGSEHG-NC----SASTGQCECKPAYAGLSCDKCQVGYFGD-----DCKFCNCDPMGTEGGVCDQTTGQCLCKEGFAGDKCDRC 597 HSP 20 Score: 55.8398 bits (133), Expect = 7.636e-7 Identity = 68/261 (26.05%), Postives = 106/261 (40.61%), Query Frame = 2
Query: 914 YYSLRNIEIGGKCI---CNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCP-----LFNQRPWKPVA-VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHG---LGTIGSCVSNGEDIGDQKPGDC-ICKKGYEGVNCDLCSIGY--ERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
Y+ +++ G C+ CN H+ CD + GI +C+HNT G +CE C N P+ +A C N + C ++ LQ NC+ G C++C GF+ + C+PCQC+G L SC N GDC +C++ +G +C+ C+ + + C C CS GS C CK++V D C CK ++ +GC+ C+C
Sbjct: 1806 YHRVQSGSFLGACVPCECNGHSATCDP--DTGICT---DCEHNTNGDHCEFCNEGHYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQC---------------NCKPGYTGDRCDRCASGFFGHPQISGES---CSPCQCNGNNNLTDSRSCHPNS--------GDCYLCEQNTDGRHCESCAAWFYGDAVTAKNCSSCECSQCGSQYCD--NKSGGCECKINVEGDS-CDRCKPDHWGFSKC--QGCQGCHC 2030 HSP 21 Score: 55.0694 bits (131), Expect = 1.432e-6 Identity = 44/151 (29.14%), Postives = 72/151 (47.68%), Query Frame = 2
Query: 4613 KKCNCN--GYSK---VCDSVT-GDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
++CNCN G ++ CD V+ G+ +C + G C C+ F+ ++ D C C C N +++ +C C+ G+ C+ C GFY N + C+SC C+ G+ C+ G C+C P V G++CDQ N+
Sbjct: 699 EECNCNPSGVTRDFQGCDKVSPGELCSCRKHVTGRICDQCKPTFWDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTG---QCMCKKNADGRRCDQCADGFYRLNSYNQMGCESCHCDIGGALRAECDITSGQCKCRPRVTGLRCDQPIENH 846
BLAST of Laminin subunit alpha-2 vs. Ensembl Fly
Match: wb (gene:FBgn0261563 transcript:FBtr0305063) HSP 1 Score: 743.806 bits (1919), Expect = 0.000e+0 Identity = 566/1920 (29.48%), Postives = 868/1920 (45.21%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIE---------FAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQ-SIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEK-IALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIE-LQSTLKGSLIDSPDVII--KCGDFRLFHSVSIEDRRLQRQV------FIYIKPTEWKI--------GKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRV--NNVLYQ--GQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLD-------------YQMTELCSSYS---SENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--------------------------------PN------CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWN--NRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYN-GQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENG--TKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC--------------------HAILNHRIVENSIYEYHIF---------DEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTE-VFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILED---SNVINSNLTRDIFMMALLNVTSLKVKLYT----DKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIK--GIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQC-APNYVVEKKTCQSCYSGCTKDLLVRID----TLNETVINFKWKTLPS---------TLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLED-----LRS----TFTDINETISGHEQLGISNELIKNATAYLETIK 5464
++ A+I+ +ATCG G E +C+ V C +C+ + + K+ I+ + S + WWQSP++ G +++ V + +DLKQ +QI + LK +SPRP +WILE+SLDG + PW +F + C QR++ +L N K D E+ C+T +S+P L +G + SL+ R ++ P+ L+ F++ RYIR R + A + L+ S+ +YSL +++ + CN HA++ + ++ + +C C+HNTCG+ CE CCPLF RP++ CE C C + C+Y+ + G+C +C NT GI CE C GFYR + T+ PC PC C+ + G C S+G G C C +G++G NC+ C+ GY +C C C GS+ S C C CK++V C C GYF+L + N EGC C+C G++ C S + L L WK+T K I++P L N + YW A YLGN +TSYG+ + V+ + ++ G P+VI+ K G F S++ + V + ++ PT I G ++ T+ +F+S LV +D +LI+A ++++Q L + G V L +E+C CP G+ GLSCE C G++R+ N +Q +C C C HSN+CD +G C +C HNT G CE C GYYG+ N CK+C CP + Y +L + Y+C +C +G HC C +GY+GNP++ SC+RC C C+ TGEC C NT G C+ CK G++GD C C C + GS S CD G+C C+ + ++CD C G + +C CNC+ G + ++ C+ +GQC C E V G +C +C +GYF PN C++C CS +LS+DCD G+C C +NV R CDKC H WN C C C GS +C+ TG C C V + C++C G+FG GC++C C + GQVC+ + G C+CP T G C +C +W + +GC++C C GS C+ G C C+ + G KCD C GY+G+P C+ C C G T+++ CD GQC CK V+G KC C + TF GC CFCFG S +C I H V +S YEY + E ++ D S + + G + P+Y + F S + ++ + + R I Q V + +++ L +DF E+ + +Y ++ + + E + + + R+ M AL N+ + ++ + +V + + Y + +T I K VE+C C G CQ+P +Y+ N+T S+ + ++ C+CNG S CD TG CQNC GN G+ C C EGFY + + C+ C CP + NFA+ C + E+ C C+ GY+G+ CE CQ G++G+P++ C+ C+C+ G Q+ C+ E G C C GV G+KCD+C A + + C+ C CT LL ++ L + N +P+ + + N + + L + LK E +++FQ K A E R + V + LRS T +D++ L + L K A YL+ I+
Sbjct: 160 VVPRAQISVNATCGQNGAEEYCKQVGAKP------------CGICNAHSSDRAKQRSIQSLISSGSGSGSGSGFEEGWWQSPTLQGGRQFEYVTILLDLKQTFQIFSVWLKSANSPRPASWILEKSLDGINFEPWQYFGLSDADC----QRRW-NLS-GQNGKYVFQNDTEIICSTQFSKPGPLENGVLHASLLKNRPGATDQS-------PE-LMKFITTRYIRIRLQGMHSTANQDNSLDWLLDSPSLEKHSFYSLSQLKVSARLDCNGHANRSHESPDD--PLMQCICQHNTCGAQCEQCCPLFQDRPYQMGGECEICQCYGHAESCTYDPFLDK--------------GICQSCSNNTAGIECEFCEMGFYRELDAPLTD--PCLPCSCNPARSTGGCQSDG--------GSCNCLEGFQGKNCEECAPGYY---GDDCKRCECDERGSL-GSTGSCSGVCQCKLNVEGS-TCSECAPGYFDLSAENAEGCTSCWCSGVSQTCHSAKLQTLAFETLNDWKITDIQRVKPISIPVDAETNRLIFAN-----ELDEVEAIYWQASLGYLGNRLTSYGSRLQLVLSWDVIRGDRSGKPTTGPNVILVGKNGLKIAFGDESLDGLGINLNVTLTEVGWYHVPPTVVDIKTRLRRTEGGDYHGESVTRSQFLSVLVSLDAVLIRAAFHTDQGETSLERAVIYSGGVELGGKSSSQVEQCLCPAGYTGLSCEGCAFGFKRIYENTSDHQILSKCIPCPCNGHSNSCDLQSGNCGDCMHNTFGDRCERCQLGYYGNPLQGTPNDCKRCACPLSEDSNNFSPSCQLKSYNYMDLNPQFELIEHAEYICTQCPEGYTGDHCQVCDDGYFGNPRQPGSSCQRCDCAGG--PCNVTTGECITCRGNTEGWHCERCKLGYWGDPAV-GCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYANVELRCPSCNCDPLGSLVQDR-CDPHTGQCHCKEGVMGAKCHECQDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGH-WNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDS-NGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRSGECEQSDLSWGHIRMAESRNLSVQQIRPHS-VPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSLVPKSTGNV-SIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKLL-----------------LDFYEYEE------FEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNVHMDAAIY-IKGSTNLIAK---GVERCKCPKRFDGLSCQDPGRSFYR----WRNTTIVESVFIED-LIGRAAPCHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDG--EVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKIC-DNCTLLLLDYMELVGNKLRRGMHNMDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQDYDSADILKLEAHAENLKFQSRKAVATIGKREFAIKSMREDAVTQQHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTAL-KQARFYLQAIR 1973
BLAST of Laminin subunit alpha-2 vs. Ensembl Fly
Match: wb (gene:FBgn0261563 transcript:FBtr0302731) HSP 1 Score: 741.88 bits (1914), Expect = 0.000e+0 Identity = 566/1920 (29.48%), Postives = 868/1920 (45.21%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIE---------FAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQ-SIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEK-IALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIE-LQSTLKGSLIDSPDVII--KCGDFRLFHSVSIEDRRLQRQV------FIYIKPTEWKI--------GKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRV--NNVLYQ--GQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLD-------------YQMTELCSSYS---SENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--------------------------------PN------CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWN--NRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYN-GQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENG--TKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC--------------------HAILNHRIVENSIYEYHIF---------DEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTE-VFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILED---SNVINSNLTRDIFMMALLNVTSLKVKLYT----DKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIK--GIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQC-APNYVVEKKTCQSCYSGCTKDLLVRID----TLNETVINFKWKTLPS---------TLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLED-----LRS----TFTDINETISGHEQLGISNELIKNATAYLETIK 5464
++ A+I+ +ATCG G E +C+ V C +C+ + + K+ I+ + S + WWQSP++ G +++ V + +DLKQ +QI + LK +SPRP +WILE+SLDG + PW +F + C QR++ +L N K D E+ C+T +S+P L +G + SL+ R ++ P+ L+ F++ RYIR R + A + L+ S+ +YSL +++ + CN HA++ + ++ + +C C+HNTCG+ CE CCPLF RP++ CE C C + C+Y+ + G+C +C NT GI CE C GFYR + T+ PC PC C+ + G C S+G G C C +G++G NC+ C+ GY +C C C GS+ S C C CK++V C C GYF+L + N EGC C+C G++ C S + L L WK+T K I++P L N + YW A YLGN +TSYG+ + V+ + ++ G P+VI+ K G F S++ + V + ++ PT I G ++ T+ +F+S LV +D +LI+A ++++Q L + G V L +E+C CP G+ GLSCE C G++R+ N +Q +C C C HSN+CD +G C +C HNT G CE C GYYG+ N CK+C CP + Y +L + Y+C +C +G HC C +GY+GNP++ SC+RC C C+ TGEC C NT G C+ CK G++GD C C C + GS S CD G+C C+ + ++CD C G + +C CNC+ G + ++ C+ +GQC C E V G +C +C +GYF PN C++C CS +LS+DCD G+C C +NV R CDKC H WN C C C GS +C+ TG C C V + C++C G+FG GC++C C + GQVC+ + G C+CP T G C +C +W + +GC++C C GS C+ G C C+ + G KCD C GY+G+P C+ C C G T+++ CD GQC CK V+G KC C + TF GC CFCFG S +C I H V +S YEY + E ++ D S + + G + P+Y + F S + ++ + + R I Q V + +++ L +DF E+ + +Y ++ + + E + + + R+ M AL N+ + ++ + +V + + Y + +T I K VE+C C G CQ+P +Y+ N+T S+ + ++ C+CNG S CD TG CQNC GN G+ C C EGFY + + C+ C CP + NFA+ C + E+ C C+ GY+G+ CE CQ G++G+P++ C+ C+C+ G Q+ C+ E G C C GV G+KCD+C A + + C+ C CT LL ++ L + N +P+ + + N + + L + LK E +++FQ K A E R + V + LRS T +D++ L + L K A YL+ I+
Sbjct: 160 VVPRAQISVNATCGQNGAEEYCKQVGAKP------------CGICNAHSSDRAKQRSIQSLISSGSGSGSGSGFEEGWWQSPTLQGGRQFEYVTILLDLKQTFQIFSVWLKSANSPRPASWILEKSLDGINFEPWQYFGLSDADC----QRRW-NLS-GQNGKYVFQNDTEIICSTQFSKPGPLENGVLHASLLKNRPGATDQS-------PE-LMKFITTRYIRIRLQGMHSTANQDNSLDWLLDSPSLEKHSFYSLSQLKVSARLDCNGHANRSHESPDD--PLMQCICQHNTCGAQCEQCCPLFQDRPYQMGGECEICQCYGHAESCTYDPFLDK--------------GICQSCSNNTAGIECEFCEMGFYRELDAPLTD--PCLPCSCNPARSTGGCQSDG--------GSCNCLEGFQGKNCEECAPGYY---GDDCKRCECDERGSL-GSTGSCSGVCQCKLNVEGS-TCSECAPGYFDLSAENAEGCTSCWCSGVSQTCHSAKLQTLAFETLNDWKITDIQRVKPISIPVDAETNRLIFAN-----ELDEVEAIYWQASLGYLGNRLTSYGSRLQLVLSWDVIRGDRSGKPTTGPNVILVGKNGLKIAFGDESLDGLGINLNVTLTEVGWYHVPPTVVDIKTRLRRTEGGDYHGESVTRSQFLSVLVSLDAVLIRAAFHTDQGETSLERAVIYSGGVELGGKSSSQVEQCLCPAGYTGLSCEGCAFGFKRIYENTSDHQILSKCIPCPCNGHSNSCDLQSGNCGDCMHNTFGDRCERCQLGYYGNPLQGTPNDCKRCACPLSEDSNNFSPSCQLKSYNYMDLNPQFELIEHAEYICTQCPEGYTGDHCQVCDDGYFGNPRQPGSSCQRCDCAGG--PCNVTTGECITCRGNTEGWHCERCKLGYWGDPAV-GCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYANVELRCPSCNCDPLGSLVQDR-CDPHTGQCHCKEGVMGAKCHECQDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGH-WNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDS-NGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRSGECEQSDLSWGHIRMAESRNLSVQQIRPHS-VPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSLVPKSTGNV-SIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKLL-----------------LDFYEYEE------FEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNVHMDAAIY-IKGSTNLIAK---GVERCKCPKRFDGLSCQDPGRSFYR----WRNTTIVESVFIED-LIGRAAPCHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDG--EVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKIC-DNCTLLLLDYMELVGNKLRRGMHNMDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQDYDSADILKLEAHAENLKFQSRKAVATIGKREFAIKSMREDAVTQQHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTAL-KQARFYLQAIR 1973
BLAST of Laminin subunit alpha-2 vs. Ensembl Fly
Match: LanB2 (gene:FBgn0267348 transcript:FBtr0345031) HSP 1 Score: 393.275 bits (1009), Expect = 1.187e-110 Identity = 297/1005 (29.55%), Postives = 464/1005 (46.17%), Query Frame = 2
Query: 386 WWQSPSMAQGLKYQN-VNVTIDLKQVYQIVYILLKMGDSPRPGNW-ILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKC-DKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWK-----PVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCN-------CKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKG--------WKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSV-----SIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQG---QCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKI----SCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT---KSCKKCDCDSLGSTSEN-----CDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSA-DCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPC 3229
WWQS +M +G+++ N VN+T+ L + Y I Y+ + + SPRP ++ I +R+ + ++P+ F++ T Y+L PD + + + A CT+ YS L GEI S + GR S +N E L +++A IR + T +L + + + Y+Y++ +I +G +C CN HA KC +G C C+HNT G +C+ C PL+N WK V C+ CNCN +++C ++ N R GG C++C+EN +G NCE+C + FY + + Y C C C +G+ C S+G+ C CK G G CD C Y ++ C C C GS + + P C+ CK +V C CK G+FNLD N GC PC+C+G T++C ++A I + W ++ + K+ + + +E Y+ AP +LG+ SY ++ +++ Q G DVI++ R+ + I D+ ++ F + ++ + P ++A F+S L + + I+A Y ++I L ++ L A IE+C CP G+ G CESC GYR ++ G C C C+ H++ CDS G C C HNT G +C+ CA G+YG+A N CK+CPCP C + + +C +C G C++C +G++G+P + +CK C CN N + +C+ TGEC +C +NT G C C G +GD L C +C C G+ + CD++TG+C C+ N + R C C G + C C C+ G +S C+ SGQC C V G RC++C ++ + + CK C C CD NG+C C NV R CD+C+ Y +R DC C
Sbjct: 130 WWQSETMFEGIQHPNYVNLTLHLGKSYDITYVRI-LFRSPRPESFTIYKRTSESGPWIPYQFYSATCRDTYSL-----PDSRAIRKGEGEAHA----LCTSEYSDISPLRDGEIAFSTLEGRPSGINFERSG------ELQEWVTATDIRITLDRLNTFGDEL-----FGDSQVLKSYFYAISDIAVGARCKCNGHASKCVPSTGMHGERTLVCECRHNTDGPDCDRCLPLYNDLKWKRSTSTEVNECKACNCNGLADKCFFD---------ANLFNRTGHGGHCLDCRENRDGPNCERCKENFY-----MRDDGY-CVNCACDPVGSRSLQCNSHGK---------CQCKPGVTGDKCDRCDNNYYQFGPHGCQQCGCDSGGSHQNT-----PACDTETGICFCKENVEGR-RCNECKPGFFNLDKNNRFGCTPCFCYGHTSEC--MTAPGYSIVSVTSNFNKFKERWTAADLNQREVDI-KYNQYSRSIGTTAQGNEHV------YFQAPDRFLGDQRASYNRDLKFKLQLVGQVANTG----VSDVILEGAGSRISLPIFAQGNGIPDQGVKEYTFRLHEHHDY---QWQPSQSARG--FLSILSNLTAIKIRATYSVQGEAI-LDDVELQTAHRGAAGHPATWIEQCTCPEGYLGQFCESCAPGYR--HSPARGGPFMPCIPCDCHGHADICDSETGRCI-CQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPN-----DGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGNGCENCLCDPVGSYNST--CDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKGFQCD-QNGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDC 1053 HSP 2 Score: 146.747 bits (369), Expect = 2.137e-34 Identity = 161/615 (26.18%), Postives = 240/615 (39.02%), Query Frame = 2
Query: 3281 CKCAQNVARRDCSKC-----DVGYFGLDVTGCKKCPPC----------YNGQVCN---NEGICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEK-NSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGI----PCKSCDCNQQGSQSL--NCNE-FGIC-QCSPGVLGIKCDQC 5026
C+C N DC +C D+ + T +C C ++ + N + G C+ C N G C +C E+ + ++ C C C GS S CN G C CK G KCDRC+ YY F CQ C C G+ N CD TG C CK+NV G +C C+ G F D GC CFC+G + +C + IV + ++ F E+ + + + I N Y R G +Y F + + R + + + G ++V +E + G + Q + + D S R F+ L N+T++K++ Y+ + L +++ T A G T +E+C C G C++ GY + R G + C+C+G++ +CDS TG C C N G+ C C +GFY N + C+ C CP+ C +I + +C GY G CE C GF+G+P + CKSCDCN + NCN G C +C G CDQC
Sbjct: 322 CECRHNTDGPDCDRCLPLYNDLKWKRSTSTEVNECKACNCNGLADKCFFDANLFNRTGHGGHCLDCRENRDGPNCERCKENFYMRDDGY-CVNCACDPVGSRSLQCNS-HGKCQCKPGVTGDKCDRCDNNYYQFGPHGCQQCGCDSGGSHQN-TPACDTETGICFCKENVEGRRCNECKPGFFNLDKNNRFGCTPCFCYGHTSECMTAPGYSIV-SVTSNFNKFKERWTAADLNQREVDIKYN-QYSRSIGTTAQGNEHVYFQAPDRFLGDQRASYNRDLKFKLQLVGQVANTG-----VSDVILEGAGSRISLPIFA-------------------QGNGIPDQGVKEYTFRLHEHHDYQWQPSQSARG-FLSILSNLTAIKIRATYSVQGEAILDDVELQTAH----RGAAGHPATWIEQCTCPEGYLGQFCESCAPGY-----------RHSPARGG--PFMPCIPCDCHGHADICDSETGRCI-CQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGA-----CLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQC 883 HSP 3 Score: 145.591 bits (366), Expect = 5.220e-34 Identity = 105/340 (30.88%), Postives = 159/340 (46.76%), Query Frame = 2
Query: 2594 GVHCDRCIEGYYGNPKKK---ISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCT-------SGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSAD------CDLNNGKCKCGSNVINRICDKCENLHYWNNRT--DCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC-NCY 3514
G C+ C GY +P + + C C C+ + + CD TG C C +NT G C C GFYG+AL + CK+C C + G+ + + +C + +C+ C+ +GL + + C C+C ++ CN T+G+C C+ N G+ C++C+ G+F P C +C C D CD G+C+C NVI R C +C+ Y+N R+ C C C GS N CD +G C C V + C +C+ ++G GCK C G C+ G C C N G C++C E+ +D + GC C +CY
Sbjct: 718 GQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADICDSETGRCICQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQ-PGYFNIRSGNGCENCLCDPVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKGFQCDQNGQCPCNDNVEGRRCDRCKENKYDRHR--GCIDCPDCY 1054 HSP 4 Score: 142.895 bits (359), Expect = 3.141e-33 Identity = 105/357 (29.41%), Postives = 162/357 (45.38%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKDKK------CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN-----YPN-CKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWN------NRTDCVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFG----LDVTGCKKCPPCYNGQVCNNE----------GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC 3805
+C C ++ + C+SC G + CIPC+C I C++ +G+C C N +GD C++C +G++ PN CK+C C + + C +C C++C + + + C C+C G+++P C+ TG C KC N A C +C G+FG L C +C CY +E G C C PN IG C +C ++ GC+ C C GSY++ C++ SG C C+ G +CD+C +YGF + C+PC C E+G+K +CD+ GQC C NV G +C C++ + GC C
Sbjct: 709 QCTCPEGYLGQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADI-----CDSETGRCICQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCN-GNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCS-CYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGNGCENCLCDPVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKG---FQCDQ-NGQCPCNDNVEGRRCDRCKENKY----DRHRGCIDC 1050 HSP 5 Score: 112.849 bits (281), Expect = 4.117e-24 Identity = 156/656 (23.78%), Postives = 266/656 (40.55%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNST-------KSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKK---TCQSCYS----------------GCTKDLLVRIDTLNETVINFKWKTLPSTL-----DLR-------------LNKINISIQMSSELLNSINKFYKLKN-EI----KNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRS---TFTDINETIS--GHEQLGISNELIKNATAYLETIKNIYKEMPNI-TLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLD----AISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQ---QLNKSSNLIKKLTKDFNFLH----NAEQAKEAYSNITRDILAVDYAINQTKKELD-----FNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQ 6226
KCI N T G HC +SG++ + LP+ ++G+ + I CD VTG CQ C N G C C+ G++ N ++ + CE C C P YN C R +C CR G G+ C+ C+ FYG +G CK C+C++ GS+ C++ G C C+ V G +CD+C N + C CY+ T D + R N+ K K + + D R ++ ++ + E L S +KF N EI +N++I Q ++ KE ++ L + L + F +E IS E ++++L A L+ K+ + LA S + LK EL + SH++ ++ K A+E+ LN ++ ++ + E+ I L+K ++ D L K+I +L + F+ E++L ++ E + L L+ ++ D K VEQ L +++N +KL + + +AE+A + NI ++I + I+Q ++ LD N + N + AE S +E++ N K + L++
Sbjct: 870 KCIHN--TAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSIT-------------RCDQVTGQCQ-CKPNVIGRDCGECQPGYF-NIRSGNGCENCLCDPVGSYN--STCDRYSG----QCHCRPGVMGQRCDQCENYFYGFSSEG--CKPCECDESGSKGFQCDQNGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDCYNLVQDAADLHRAKLFNLSQTLDEIARTPVTNDDEFEAKLKAVQEKVAVLAQDARDNSGDGGQTYAEVIDDLHKHLDSVREHLVSADKFQADANGEIDRARQNYTILDQITENAKKELQQALDLLNDEGAQALARAKEKSVEFGQQSEQISDISREARALADKLESEAQFDLKNAKDAKDAVEKAHQLAKSAIDLQLKIGTELRSEVGLELSHVKQSLGTVVQTSKEALRKANEVYDTALTLLNDVN-RQTQPEIDI-------SQLKKDAVAANERADELLKQITELSNSNGELFADFETEQELTEALLKRAE--QQQLEDIELLERAKAAHDKATKAVEQGDNTLKEANNTYEKLAGFQSDVQRSSESAEKALQTVPNIEKEIQNAESLISQAEEALDGANKNANEAKKNAQEAQLKYAEQASKDAELIRRKANETKVAARNLRE 1490 HSP 6 Score: 91.6633 bits (226), Expect = 1.199e-17 Identity = 80/263 (30.42%), Postives = 103/263 (39.16%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-------CRCSYNTMGTECQLCKHGFYGDALTK--------SCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYPN--CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLN---PKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY 3382
RCKCN + C P TG C C +NT G +C C Y D K CK C NC+ L KC N NR G C C EN +G CE+C E ++ + C C C P + S C+ ++GKC+C V CD+C+N +Y C C C G + P CD ETG C C +NV R C++C G+F LD C PC+
Sbjct: 298 RCKCNGHASKCVPSTGMHGERTLVCECRHNTDGPDCDRCLP-LYNDLKWKRSTSTEVNECKAC----------NCNGLADKCFFDANLFNR---------------------------------TGHGGHCLDCRENRDGPNCERCKENFYMRDDGYCVNCACDPVGSRSLQCN-SHGKCQCKPGVTGDKCDRCDNNYYQFGPHGCQQCGCDSGGSHQNTPACDTETGICFCKENVEGRRCNECKPGFFNLDKNNRFGCTPCF 515 HSP 7 Score: 69.3218 bits (168), Expect = 6.732e-11 Identity = 49/154 (31.82%), Postives = 69/154 (44.81%), Query Frame = 2
Query: 4613 KKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQ--SLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
K CNCNG + C G C +C NR+G C+ C+E FY D Y C C C ++ + KCQC+ G +G C+ C +Y G C+ C C+ GS + C+ E GIC C V G +C++C P +
Sbjct: 357 KACNCNGLADKCFFDANLFNRTGHGGHCLDCRENRDGPNCERCKENFYMRDDGY--CVNCACDPV------GSRSLQCNSHGKCQCKPGVTGDKCDRCDNNYYQFGPHG--CQQCGCDSGGSHQNTPACDTETGICFCKENVEGRRCNECKPGF 500 HSP 8 Score: 59.3066 bits (142), Expect = 7.784e-8 Identity = 37/81 (45.68%), Postives = 44/81 (54.32%), Query Frame = 2
Query: 4841 CRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSCYSGC 5080
CR G +CE C++ FY G C +C C+ GS+SL CN G CQC PGV G KCD+C NY CQ C GC
Sbjct: 387 CRENRDGPNCERCKENFYMRD-DGY-CVNCACDPVGSRSLQCNSHGKCQCKPGVTGDKCDRCDNNYYQFGPHGCQQC--GC 463
BLAST of Laminin subunit alpha-2 vs. Ensembl Fly
Match: LanB2 (gene:FBgn0267348 transcript:FBtr0076382) HSP 1 Score: 393.275 bits (1009), Expect = 1.187e-110 Identity = 297/1005 (29.55%), Postives = 464/1005 (46.17%), Query Frame = 2
Query: 386 WWQSPSMAQGLKYQN-VNVTIDLKQVYQIVYILLKMGDSPRPGNW-ILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKC-DKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWK-----PVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCN-------CKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKG--------WKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSV-----SIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQG---QCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKI----SCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT---KSCKKCDCDSLGSTSEN-----CDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSA-DCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPC 3229
WWQS +M +G+++ N VN+T+ L + Y I Y+ + + SPRP ++ I +R+ + ++P+ F++ T Y+L PD + + + A CT+ YS L GEI S + GR S +N E L +++A IR + T +L + + + Y+Y++ +I +G +C CN HA KC +G C C+HNT G +C+ C PL+N WK V C+ CNCN +++C ++ N R GG C++C+EN +G NCE+C + FY + + Y C C C +G+ C S+G+ C CK G G CD C Y ++ C C C GS + + P C+ CK +V C CK G+FNLD N GC PC+C+G T++C ++A I + W ++ + K+ + + +E Y+ AP +LG+ SY ++ +++ Q G DVI++ R+ + I D+ ++ F + ++ + P ++A F+S L + + I+A Y ++I L ++ L A IE+C CP G+ G CESC GYR ++ G C C C+ H++ CDS G C C HNT G +C+ CA G+YG+A N CK+CPCP C + + +C +C G C++C +G++G+P + +CK C CN N + +C+ TGEC +C +NT G C C G +GD L C +C C G+ + CD++TG+C C+ N + R C C G + C C C+ G +S C+ SGQC C V G RC++C ++ + + CK C C CD NG+C C NV R CD+C+ Y +R DC C
Sbjct: 130 WWQSETMFEGIQHPNYVNLTLHLGKSYDITYVRI-LFRSPRPESFTIYKRTSESGPWIPYQFYSATCRDTYSL-----PDSRAIRKGEGEAHA----LCTSEYSDISPLRDGEIAFSTLEGRPSGINFERSG------ELQEWVTATDIRITLDRLNTFGDEL-----FGDSQVLKSYFYAISDIAVGARCKCNGHASKCVPSTGMHGERTLVCECRHNTDGPDCDRCLPLYNDLKWKRSTSTEVNECKACNCNGLADKCFFD---------ANLFNRTGHGGHCLDCRENRDGPNCERCKENFY-----MRDDGY-CVNCACDPVGSRSLQCNSHGK---------CQCKPGVTGDKCDRCDNNYYQFGPHGCQQCGCDSGGSHQNT-----PACDTETGICFCKENVEGR-RCNECKPGFFNLDKNNRFGCTPCFCYGHTSEC--MTAPGYSIVSVTSNFNKFKERWTAADLNQREVDI-KYNQYSRSIGTTAQGNEHV------YFQAPDRFLGDQRASYNRDLKFKLQLVGQVANTG----VSDVILEGAGSRISLPIFAQGNGIPDQGVKEYTFRLHEHHDY---QWQPSQSARG--FLSILSNLTAIKIRATYSVQGEAI-LDDVELQTAHRGAAGHPATWIEQCTCPEGYLGQFCESCAPGYR--HSPARGGPFMPCIPCDCHGHADICDSETGRCI-CQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPN-----DGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGNGCENCLCDPVGSYNST--CDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKGFQCD-QNGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDC 1053 HSP 2 Score: 146.747 bits (369), Expect = 2.137e-34 Identity = 161/615 (26.18%), Postives = 240/615 (39.02%), Query Frame = 2
Query: 3281 CKCAQNVARRDCSKC-----DVGYFGLDVTGCKKCPPC----------YNGQVCN---NEGICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEK-NSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGI----PCKSCDCNQQGSQSL--NCNE-FGIC-QCSPGVLGIKCDQC 5026
C+C N DC +C D+ + T +C C ++ + N + G C+ C N G C +C E+ + ++ C C C GS S CN G C CK G KCDRC+ YY F CQ C C G+ N CD TG C CK+NV G +C C+ G F D GC CFC+G + +C + IV + ++ F E+ + + + I N Y R G +Y F + + R + + + G ++V +E + G + Q + + D S R F+ L N+T++K++ Y+ + L +++ T A G T +E+C C G C++ GY + R G + C+C+G++ +CDS TG C C N G+ C C +GFY N + C+ C CP+ C +I + +C GY G CE C GF+G+P + CKSCDCN + NCN G C +C G CDQC
Sbjct: 322 CECRHNTDGPDCDRCLPLYNDLKWKRSTSTEVNECKACNCNGLADKCFFDANLFNRTGHGGHCLDCRENRDGPNCERCKENFYMRDDGY-CVNCACDPVGSRSLQCNS-HGKCQCKPGVTGDKCDRCDNNYYQFGPHGCQQCGCDSGGSHQN-TPACDTETGICFCKENVEGRRCNECKPGFFNLDKNNRFGCTPCFCYGHTSECMTAPGYSIV-SVTSNFNKFKERWTAADLNQREVDIKYN-QYSRSIGTTAQGNEHVYFQAPDRFLGDQRASYNRDLKFKLQLVGQVANTG-----VSDVILEGAGSRISLPIFA-------------------QGNGIPDQGVKEYTFRLHEHHDYQWQPSQSARG-FLSILSNLTAIKIRATYSVQGEAILDDVELQTAH----RGAAGHPATWIEQCTCPEGYLGQFCESCAPGY-----------RHSPARGG--PFMPCIPCDCHGHADICDSETGRCI-CQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGA-----CLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQC 883 HSP 3 Score: 145.591 bits (366), Expect = 5.220e-34 Identity = 105/340 (30.88%), Postives = 159/340 (46.76%), Query Frame = 2
Query: 2594 GVHCDRCIEGYYGNPKKK---ISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCT-------SGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSAD------CDLNNGKCKCGSNVINRICDKCENLHYWNNRT--DCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC-NCY 3514
G C+ C GY +P + + C C C+ + + CD TG C C +NT G C C GFYG+AL + CK+C C + G+ + + +C + +C+ C+ +GL + + C C+C ++ CN T+G+C C+ N G+ C++C+ G+F P C +C C D CD G+C+C NVI R C +C+ Y+N R+ C C C GS N CD +G C C V + C +C+ ++G GCK C G C+ G C C N G C++C E+ +D + GC C +CY
Sbjct: 718 GQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADICDSETGRCICQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQ-PGYFNIRSGNGCENCLCDPVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKGFQCDQNGQCPCNDNVEGRRCDRCKENKYDRHR--GCIDCPDCY 1054 HSP 4 Score: 142.895 bits (359), Expect = 3.141e-33 Identity = 105/357 (29.41%), Postives = 162/357 (45.38%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKDKK------CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN-----YPN-CKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWN------NRTDCVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFG----LDVTGCKKCPPCYNGQVCNNE----------GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC 3805
+C C ++ + C+SC G + CIPC+C I C++ +G+C C N +GD C++C +G++ PN CK+C C + + C +C C++C + + + C C+C G+++P C+ TG C KC N A C +C G+FG L C +C CY +E G C C PN IG C +C ++ GC+ C C GSY++ C++ SG C C+ G +CD+C +YGF + C+PC C E+G+K +CD+ GQC C NV G +C C++ + GC C
Sbjct: 709 QCTCPEGYLGQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADI-----CDSETGRCICQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCN-GNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCS-CYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGNGCENCLCDPVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKG---FQCDQ-NGQCPCNDNVEGRRCDRCKENKY----DRHRGCIDC 1050 HSP 5 Score: 112.849 bits (281), Expect = 4.117e-24 Identity = 156/656 (23.78%), Postives = 266/656 (40.55%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNST-------KSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKK---TCQSCYS----------------GCTKDLLVRIDTLNETVINFKWKTLPSTL-----DLR-------------LNKINISIQMSSELLNSINKFYKLKN-EI----KNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRS---TFTDINETIS--GHEQLGISNELIKNATAYLETIKNIYKEMPNI-TLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLD----AISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQ---QLNKSSNLIKKLTKDFNFLH----NAEQAKEAYSNITRDILAVDYAINQTKKELD-----FNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQ 6226
KCI N T G HC +SG++ + LP+ ++G+ + I CD VTG CQ C N G C C+ G++ N ++ + CE C C P YN C R +C CR G G+ C+ C+ FYG +G CK C+C++ GS+ C++ G C C+ V G +CD+C N + C CY+ T D + R N+ K K + + D R ++ ++ + E L S +KF N EI +N++I Q ++ KE ++ L + L + F +E IS E ++++L A L+ K+ + LA S + LK EL + SH++ ++ K A+E+ LN ++ ++ + E+ I L+K ++ D L K+I +L + F+ E++L ++ E + L L+ ++ D K VEQ L +++N +KL + + +AE+A + NI ++I + I+Q ++ LD N + N + AE S +E++ N K + L++
Sbjct: 870 KCIHN--TAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSIT-------------RCDQVTGQCQ-CKPNVIGRDCGECQPGYF-NIRSGNGCENCLCDPVGSYN--STCDRYSG----QCHCRPGVMGQRCDQCENYFYGFSSEG--CKPCECDESGSKGFQCDQNGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDCYNLVQDAADLHRAKLFNLSQTLDEIARTPVTNDDEFEAKLKAVQEKVAVLAQDARDNSGDGGQTYAEVIDDLHKHLDSVREHLVSADKFQADANGEIDRARQNYTILDQITENAKKELQQALDLLNDEGAQALARAKEKSVEFGQQSEQISDISREARALADKLESEAQFDLKNAKDAKDAVEKAHQLAKSAIDLQLKIGTELRSEVGLELSHVKQSLGTVVQTSKEALRKANEVYDTALTLLNDVN-RQTQPEIDI-------SQLKKDAVAANERADELLKQITELSNSNGELFADFETEQELTEALLKRAE--QQQLEDIELLERAKAAHDKATKAVEQGDNTLKEANNTYEKLAGFQSDVQRSSESAEKALQTVPNIEKEIQNAESLISQAEEALDGANKNANEAKKNAQEAQLKYAEQASKDAELIRRKANETKVAARNLRE 1490 HSP 6 Score: 91.6633 bits (226), Expect = 1.199e-17 Identity = 80/263 (30.42%), Postives = 103/263 (39.16%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-------CRCSYNTMGTECQLCKHGFYGDALTK--------SCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYPN--CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLN---PKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY 3382
RCKCN + C P TG C C +NT G +C C Y D K CK C NC+ L KC N NR G C C EN +G CE+C E ++ + C C C P + S C+ ++GKC+C V CD+C+N +Y C C C G + P CD ETG C C +NV R C++C G+F LD C PC+
Sbjct: 298 RCKCNGHASKCVPSTGMHGERTLVCECRHNTDGPDCDRCLP-LYNDLKWKRSTSTEVNECKAC----------NCNGLADKCFFDANLFNR---------------------------------TGHGGHCLDCRENRDGPNCERCKENFYMRDDGYCVNCACDPVGSRSLQCN-SHGKCQCKPGVTGDKCDRCDNNYYQFGPHGCQQCGCDSGGSHQNTPACDTETGICFCKENVEGRRCNECKPGFFNLDKNNRFGCTPCF 515 HSP 7 Score: 69.3218 bits (168), Expect = 6.732e-11 Identity = 49/154 (31.82%), Postives = 69/154 (44.81%), Query Frame = 2
Query: 4613 KKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQ--SLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
K CNCNG + C G C +C NR+G C+ C+E FY D Y C C C ++ + KCQC+ G +G C+ C +Y G C+ C C+ GS + C+ E GIC C V G +C++C P +
Sbjct: 357 KACNCNGLADKCFFDANLFNRTGHGGHCLDCRENRDGPNCERCKENFYMRDDGY--CVNCACDPV------GSRSLQCNSHGKCQCKPGVTGDKCDRCDNNYYQFGPHG--CQQCGCDSGGSHQNTPACDTETGICFCKENVEGRRCNECKPGF 500 HSP 8 Score: 59.3066 bits (142), Expect = 7.784e-8 Identity = 37/81 (45.68%), Postives = 44/81 (54.32%), Query Frame = 2
Query: 4841 CRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSCYSGC 5080
CR G +CE C++ FY G C +C C+ GS+SL CN G CQC PGV G KCD+C NY CQ C GC
Sbjct: 387 CRENRDGPNCERCKENFYMRD-DGY-CVNCACDPVGSRSLQCNSHGKCQCKPGVTGDKCDRCDNNYYQFGPHGCQQC--GC 463
BLAST of Laminin subunit alpha-2 vs. Ensembl Fly
Match: LanA (gene:FBgn0002526 transcript:FBtr0077014) HSP 1 Score: 313.538 bits (802), Expect = 9.129e-85 Identity = 201/552 (36.41%), Postives = 289/552 (52.36%), Query Frame = 2
Query: 188 LMENAKITSSATCG--TGGPEIFCRLVDEIAIQRLINPD--SHVLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCD-KVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWK------PVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPP----CNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC---FGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLT 1786
L KI ++ATCG T GPE++C+LV I+ +CD CD + HP E A D WWQSP +++G+K+ VN+TI+ +Q + + Y+ ++MG+SPRPG W LE+S D G+ + PW F++TP C F + KP + DD++ CTT YS+ L +GEI + L+N R S N Y + L + A +R R L+ L LM+ + ++ RY+YS+++I IGG+C+CN HAD CD K ++ + + C C+H+TCG C CCP F Q+ W+ P CE CNC+ SNEC Y++ V LS++ G +GGGVC NCQ NT GINC KC +YRP E C+PCQC + G C +++ G+C C+ ++ +CD C+ GY YPN C C C+++G T+ Y C+ C CK++ + CK C GY+ PE C+ C C ITN C N+ + K LT F G+ K F Y T
Sbjct: 31 LATGRKIYATATCGQDTDGPELYCKLVGANTEHDHIDYSVIQGQVCDYCDPTVPERNHPPENA-IDGTEAWWQSPPLSRGMKFNEVNLTINFEQEFHVAYLFIRMGNSPRPGLWTLEKSTDYGKTWTPWQHFSDTPADCETYFGKD--------TYKPIT-RDDDVICTTEYSKIVPLENGEIPVMLLNERPSSTN--YFN----STVLQEWTRATNVRIRLLRTKNLLGHLMS-VARQDPTVTRRYFYSIKDISIGGRCMCNGHADTCDVKDPKSPVRILACRCQHHTCGIQCNECCPGFEQKKWRQNTNARPFN-CEPCNCHGHSNECKYDEEVNRKGLSLDIHGHYDGGGVCQNCQHNTVGINCNKCKPKYYRPKGKHWNETDVCSPCQCDYFFSTGHC--------EEETGNCECRAAFQPPSCDSCAYGYYGYPN--CRECECNLNG---TNGYHCEAESGQQCPCKINF-AGAYCKQCAEGYYGF----PE-CKACECNKIGSITNDC------NVTTGECKC--LTNFGGDNCERCKHGYFNYPT 537 HSP 2 Score: 298.13 bits (762), Expect = 5.229e-80 Identity = 225/704 (31.96%), Postives = 320/704 (45.45%), Query Frame = 2
Query: 1208 GVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTT--FTGEKIALPKFT--SFQYLTSENS----YIDERFNNPTVYYWTAPPPYLG--NWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHL---ALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSS---YSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKK-KISCKRCKCNNN-----QESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK--KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCN 3232
G C+ C N G CEKC Y V+ C C C+ +G ++NG D G C C++ EG CD+CS GY +P+ C C C G+ + C CK +V D C C +G +NL +NP+GC C+CFG T++C S + +S LK +TT F E I + + + L +E + + N+ Y+ L N I++YG Y + S G I +PDVI+ L H+ S E + E ++ K ++ +F+ L + + I+A Y+ L ++ L+ + + +E+C+CPPG++G SCE C GY R + Y G C C C HS TCD G C C H T G HCE C SGYYG+A + C C CP L + +S S + + +C +G C+ C G+YG P+ CK C+C+ N Q SCD TGEC RC NT G C LC GFYGDA+ K+C+ CDCD LG ++ CD G C C N + +CD C Y + C C+C G S+ C+ +G C C V G +C++C ++ Y C C C+ ++ C+ N GKC+C VI CD C N C CN
Sbjct: 1434 GECM-CPPNVIGDLCEKCAPNTYGFHQVIG-----CEECACNPMG-----IANGNSQCDLFNGTCECRQNIEGRACDVCSNGYFNFPH--CEQCSCHKPGTELEVCDKIDGACFCKKNVVGRD-CDQCVDGTYNLQESNPDGCTTCFCFGKTSRCDSAYLRVYNVSLLKHVSITTPEFHEESIKFDMWPVPADEILLNETTLKADFTLREVNDERPAYFGVLDYLLNQNNHISAYGGDLAYT--LHFTSGFDGKYIVAPDVILFSEHNALVHT-SYEQPSRNEPFTNRVNIVESNFQTIS-GKPVSRADFMMVLRDLKVIFIRANYWEQTLVTHLSDVYLTLADEDADGTGEYQFLAVERCSCPPGYSGHSCEDCAPGYYRDPSGPYGGYCIPCECNGHSETCDCATGICSKCQHGTEGDHCERCVSGYYGNATNGTPGDCMICACP-LPFDSNNFATSCEISESGDQIHCECKPGYTGPRCESCANGFYGEPESIGQVCKPCECSGNINPEDQGSCDTRTGECLRCLNNTFGAACNLCAPGFYGDAIKLKNCQSCDCDDLG--TQTCDPFVGVCTCHENVIGDRCDRCKPDHYGFESGVGCRACDC---GAASNSTQCDPHTGHCACKSGVTGRQCDRCAVDHWKYEKDGCTPCNCNQGYSRGFGCNPNTGKCQCLPGVIGDRCDACPNRWVLIKDEGCQECN 2113 HSP 3 Score: 244.202 bits (622), Expect = 1.101e-63 Identity = 182/546 (33.33%), Postives = 250/546 (45.79%), Query Frame = 2
Query: 2240 IEKCNCPPGFNGLSCESCESGYR----RVNNVLYQGQCEACACYNHSNTCD----------SLN--------GECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCN---NNQESCDPVTGE-CRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSA-DCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEG--------ICVCPPNTIGNLCNKCSEDSWDFN--ETIGCKKCNCYINGSYS--NICNKISGICACKTAFFGSKCDRCNEGYYGFPN-----CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKC 3742
I C C G+ C C G+ R N CE C C+ HSN C SL+ G CQNC HNT G +C C YY K K+ N C + DY + + N C S CD C GYYG P +C+ C+CN N C+ +G+ C C N G C+ C G+YG CK C+C+ +GS + +C+ TG+C C TNF C+ C G Y C C+C+ QG + + CN SGQC C E G RC++C+ G++NYP+CK C CS SA CD N GKC C +N + C C +Y + DC+PC+C GS C+ + G C C N R C C G++ + C+ C G + G +C C G +CN+C W+ N T GC+ C+C+ +G+ S + C SG C CK G +C C +G + + C+ CSC G+ + CDKI+GQC+C + G C
Sbjct: 293 ILACRCQHHTCGIQCNECCPGFEQKKWRQNTNARPFNCEPCNCHGHSNECKYDEEVNRKGLSLDIHGHYDGGGVCQNCQHNTVGINCNKCKPKYY-RPKGKHWNETDVCSPCQCDYFFSTGHCEEETGNCECRAAFQPPS---CDSCAYGYYGYP----NCRECECNLNGTNGYHCEAESGQQCPCKINFAGAYCKQCAEGYYG---FPECKACECNKIGSITNDCNVTTGECKCLTNFGGDNCERCKHG-YFNYPTCSYCDCDNQG--TESEICNKQSGQCICREGFGGPRCDQCLPGFYNYPDCKPCNCSSTGSSAITCD-NTGKCNCLNNFAGKQCTLCTAGYY--SYPDCLPCHCDSHGSQGVSCNSD-GQCLCQPNFDGRQCDSCKEGFY--NFPSCEDCNCDPAGVIDKFAGCGSVPVGELCKCKERVTGRICNECKPLYWNLNISNTEGCEICDCWTDGTISALDTCTSKSGQCPCKPHTQGRRCQECRDGTFDLDSASLFGCKDCSCDVGGSWQ---SVCDKISGQCKCHPRITGLAC 815 HSP 4 Score: 239.965 bits (611), Expect = 1.818e-62 Identity = 199/677 (29.39%), Postives = 299/677 (44.17%), Query Frame = 2
Query: 3221 VPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYING--SYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCH---------AILNH-RIVENSIYEYHI-FDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDS--NVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGK---RVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKN--YDQCEPCQCPSAH--YNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEFGICQCSPG--------VLGIKCDQCAPNY---VVEKKTCQSC----YSGCTKDLLVRIDTLNETVI 5128
+PCNC GS + +C G C+C NV R C C Y+G CK C C N +C G C+CPPN IG+LC KC+ +++ F++ IGC++C C G + ++ C+ +G C C+ G CD C+ GY+ FP+C+ CSC + GT EL CDKI G C CK+NV+G C C GT+ + GC +CFCFG + +C ++L H I +E I FD + L A++ R + RP Y V+ + + + + ++ G+ I + +N L Y + S+N + ++ I+E + + ++R FMM L ++ + ++ Y ++ L+ Y + A G + AVE+C C G+ C++ GYY++ P+ G C CNG+S+ CD TG C C EG+ C+ C G+Y N N C C CP NFA +C ES ++ C+C+ GY+G CE+C GFYG P G CK C+C S ++N + G C G G C+ CAP + ++ K CQSC T D V + T +E VI
Sbjct: 1373 LPCNCDYAGSTSFECHPFGGQCQCKPNVIERTCGACRSRYYGF--PDCKPCK-CPNSAMCEPTTGECMCPPNVIGDLCEKCAPNTYGFHQVIGCEECACNPMGIANGNSQCDLFNGTCECRQNIEGRACDVCSNGYFNFPHCEQCSCHKPGT---ELEVCDKIDGACFCKKNVVGRDCDQCVDGTYNLQESNPDGCTTCFCFGKTSRCDSAYLRVYNVSLLKHVSITTPEFHEESIKFDMWPVPADEILLNETTLKADFTLR---EVNDERPAYFGVLDYLLNQNNHISAYGGDLAYTLHFTSGFDGKYIVAPDVILFSEHNALVHTSY----------------EQPSRNEPFT---------NRVNIVESNFQTISGKPVSRADFMMVLRDLKVIFIRANYWEQT---LVTHLSDVYLTLADEDADGTGEYQFLAVERCSCPPGYSGHSCEDCAPGYYRD----PSGPYGGY----------CIPCECNGHSETCDCATGICSKCQHGTEGDHCERCVSGYYGNATNGTPGDCMICACPLPFDSNNFATSCEISESGDQIHCECKPGYTGPRCESCANGFYGEPESIGQVCKPCEC----SGNINPEDQGSCDTRTGECLRCLNNTFGAACNLCAPGFYGDAIKLKNCQSCDCDDLGTQTCDPFVGVCTCHENVI 1994 HSP 5 Score: 229.95 bits (585), Expect = 1.960e-59 Identity = 170/534 (31.84%), Postives = 240/534 (44.94%), Query Frame = 2
Query: 2348 CACYNHSNTCDSLNGECQ------NCTHNTAGAHCEDCASGYYGDAKSKNLNS----CKKCPCP------RLDYQMTELCSSYSSENY-----VCHKCLHNTSGVHCDRCIEGYYGNPKKKI-----SCKRCKCNN--NQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGK-CDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY------NGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICN----KISGICACKTAFFGSKCDRCNEGYYGF-----PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
C C H++TCD + + C H+T G C +C G+ +N N+ C+ C C + D ++ S + VC C HNT G++C++C YY PK K C C+C+ + C+ TG C C C C +G+YG +C++C+C+ G+ +C+ +G+ C C+ NF C C G Y +C C C G I+++ CN T+G+C CL N GD CE+C GYFNYP C C C ++ C+ +G+C C CD+C L + N DC PCNC GS CD TG C C N A + C+ C GY+ C PC+ G CN++G C+C PN G C+ C E ++F C+ CNC G + +C CK G C+ C Y+ C+ C C +GT S L C +GQC CK + G +C+ CR GTF D GC C C
Sbjct: 273 CMCNGHADTCDVKDPKSPVRILACRCQHHTCGIQCNECCPGFEQKKWRQNTNARPFNCEPCNCHGHSNECKYDEEVNRKGLSLDIHGHYDGGGVCQNCQHNTVGINCNKCKPKYY-RPKGKHWNETDVCSPCQCDYFFSTGHCEEETGNCECRAAFQPPSCDSCAYGYYG---YPNCRECECNLNGTNGYHCEAESGQQCPCKINFAGAYCKQCAEGYY-GFPECKACECNKIGSITND--CNVTTGECKCLTNFGGDNCERCKHGYFNYPTCSYCDCDNQGTESEICNKQSGQCICREGFGGPRCDQC--LPGFYNYPDCKPCNCSSTGSSAITCD-NTGKCNCLNNFAGKQCTLCTAGYYSY-----PDCLPCHCDSHGSQGVSCNSDGQCLCQPNFDGRQCDSCKEGFYNFPS---CEDCNCDPAGVIDKFAGCGSVPVGELCKCKERVTGRICNECKPLYWNLNISNTEGCEICDCWTDGTIS-ALDTCTSKSGQCPCKPHTQGRRCQECRDGTFDLDSASLFGCKDCSC 787 HSP 6 Score: 149.058 bits (375), Expect = 7.056e-35 Identity = 123/418 (29.43%), Postives = 186/418 (44.50%), Query Frame = 2
Query: 2660 RCKCNNNQESCD------PV-TGECRCSYNTMGTECQLCKHGFY-------GDALTKSCKKCDCDSLGSTSENCDKLT---------------GKC-DCRTNFVNRQCDSCTSGLYLKDKK-------CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSA-DCDLNNG-KCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYN----GQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKG--TFPS 3772
RC CN + ++CD PV CRC ++T G +C C GF +A +C+ C+C + + +++ G C +C+ N V C+ C Y K C PC C+ S C +G C C C+ C GY+ YPNC++C C+ + + C+ +G +C C N C +C +Y +C C C + GS+ C++ TG CKC N +C +C GYF C C C N ++CN + G C+C G C++C +++ + CK CNC GS + C+ +G C C F G +C C GYY +P+C PC C +G++ C+ GQC C+ N G +C C++G FPS
Sbjct: 272 RCMCNGHADTCDVKDPKSPVRILACRCQHHTCGIQCNECCPGFEQKKWRQNTNARPFNCEPCNCHGHSNECKYDEEVNRKGLSLDIHGHYDGGGVCQNCQHNTVGINCNKCKPKYYRPKGKHWNETDVCSPCQCDY---FFSTGHCEEETGNCECRAAFQPPSCDSCAYGYYGYPNCRECECNLNGTNGYHCEAESGQQCPCKINFAGAYCKQCAEGYY--GFPECKACECNKIGSITNDCNVTTGECKCLTNFGGDNCERCKHGYFNYPT--CSYC-DCDNQGTESEICNKQSGQCICREGFGGPRCDQCLPGFYNYPD---CKPCNCSSTGSSAITCDN-TGKCNCLNNFAGKQCTLCTAGYYSYPDCLPCHCDSHGSQG---VSCNS-DGQCLCQPNFDGRQCDSCKEGFYNFPS 673 HSP 7 Score: 134.806 bits (338), Expect = 1.319e-30 Identity = 99/334 (29.64%), Postives = 146/334 (43.71%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKDKK-----CIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP------NCKKCLC----SPHALSADCDLNNG----KCKCGSNVINRICDKCENLHYWNNRT---DCVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC----YNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
+C C + C+ C G Y CIPC C + C+ +G C C GD CE+CV GY+ +C C C + + C+++ C+C C+ C N Y + C PC C G++NP+ CD TG C +C N C+ C G++G D K C C Q C+ G+C C N IG+ C++C D + F +GC+ C+C S S C+ +G CACK+ G +CDRC ++ + C PC+C + ++ C+ TG+CQC VIG +C C
Sbjct: 1775 RCSCPPGYSGHSCEDCAPGYYRDPSGPYGGYCIPCECN-----GHSETCDCATGICSKCQHGTEGDHCERCVSGYYGNATNGTPGDCMICACPLPFDSNNFATSCEISESGDQIHCECKPGYTGPRCESCANGFYGEPESIGQVCKPCECS-GNINPEDQGSCDTRTGECLRCLNNTFGAACNLCAPGFYG-DAIKLKNCQSCDCDDLGTQTCDPFVGVCTCHENVIGDRCDRCKPDHYGFESGVGCRACDCGA-ASNSTQCDPHTGHCACKSGVTGRQCDRCAVDHWKYEKDGCTPCNCNQGYSRG---FGCNPNTGKCQCLPGVIGDRCDAC 2097 HSP 8 Score: 134.035 bits (336), Expect = 2.163e-30 Identity = 124/388 (31.96%), Postives = 172/388 (44.33%), Query Frame = 2
Query: 2243 EKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACAC---YNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMT--ELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQES---CDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTS-GQ-CFCLENVNGDRCEKCVEGYFNY-----PNCKKCLC-SPHALSA--DCDLNNGKCKCGSNVINRICDKCENLHYWNNRTD---CVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYF 3340
++C C F G C+ C GY +C+AC C + +N C+ GEC+ C N G +CE C GY+ N +C C C D Q T E+C+ S + C G CD+C+ G+Y P CK C C++ S CD TG+C C N G +C LC G+Y C C CDS GS +C+ G+C C+ NF RQCDSC G Y C CNC+ G I C + G+ C C E V G C +C Y+N C+ C C + +SA C +G+C C + R C +C + + + C C+C GS CD +G CKC + C++ +F
Sbjct: 466 QQCPCKINFAGAYCKQCAEGYYGF------PECKACECNKIGSITNDCNVTTGECK-CLTNFGGDNCERCKHGYF------NYPTCSYCDC---DNQGTESEICNKQSGQCI----CREGFGGPRCDQCLPGFYNYP----DCKPCNCSSTGSSAITCDN-TGKCNCLNNFAGKQCTLCTAGYYS---YPDCLPCHCDSHGSQGVSCNS-DGQCLCQPNFDGRQCDSCKEGFY-NFPSCEDCNCDPAGVIDKFAGCGSVPVGELCKCKERVTGRICNECKPLYWNLNISNTEGCEICDCWTDGTISALDTCTSKSGQCPCKPHTQGRRCQECRDGTFDLDSASLFGCKDCSCDVGGSWQSVCDKISGQCKCHPRITGLACTQPLTTHF 823 HSP 9 Score: 114.39 bits (285), Expect = 2.222e-24 Identity = 102/344 (29.65%), Postives = 144/344 (41.86%), Query Frame = 2
Query: 2246 KCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNH---SNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQE---SCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTG--------KCDCRTNFVNRQCDSCTSGLYL-----KDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN-----CKKCLCS-PHALSADCDLNNGKCKCGSNVINRICDKCENLHYW 3202
+C C F G +CE C+ GY C C C N S C+ +G+C C G C+ C G+Y N CK C C + S+ + +N CL+N +G C C GYY P C C C+++ SC+ G+C C N G +C CK GFY SC+ C+CD G DK G C C+ R C+ C LY + C C+C G IS+ C + SGQC C + G RC++C +G F+ + CK C C + + CD +G+CKC + C + H++
Sbjct: 513 ECKCLTNFGGDNCERCKHGY------FNYPTCSYCDCDNQGTESEICNKQSGQCI-CREGFGGPRCDQCLPGFY------NYPDCKPCNC------SSTGSSAITCDNTGKCNCLNNFAGKQCTLCTAGYYSYP----DCLPCHCDSHGSQGVSCN-SDGQCLCQPNFDGRQCDSCKEGFYN---FPSCEDCNCDPAGVI----DKFAGCGSVPVGELCKCKERVTGRICNECKP-LYWNLNISNTEGCEICDCWTDGTISALDTCTSKSGQCPCKPHTQGRRCQECRDGTFDLDSASLFGCKDCSCDVGGSWQSVCDKISGQCKCHPRITGLACTQPLTTHFF 824 HSP 10 Score: 108.227 bits (269), Expect = 1.307e-22 Identity = 69/203 (33.99%), Postives = 103/203 (50.74%), Query Frame = 2
Query: 2936 IPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCE-NLHYWNNRTDCVPCNC---GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCP---PCYNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNET--IGCKKCNCY 3514
+PCNC+ G S E C+ GQC C NV C C Y+ +P+CK C C A+ C+ G+C C NVI +C+KC N + ++ C C C G + N +CD+ G C+C QN+ R C C GYF + C++C P +VC+ +G C C N +G C++C + +++ E+ GC C C+
Sbjct: 1373 LPCNCDYAGSTSFE--CHPFGGQCQCKPNVIERTCGACRSRYYGFPDCKPCKCPNSAM---CEPTTGECMCPPNVIGDLCEKCAPNTYGFHQVIGCEECACNPMGIANGNSQCDLFNGTCECRQNIEGRACDVCSNGYF--NFPHCEQCSCHKPGTELEVCDKIDGACFCKKNVVGRDCDQCVDGTYNLQESNPDGCTTCFCF 1568 HSP 11 Score: 96.6709 bits (239), Expect = 4.516e-19 Identity = 134/591 (22.67%), Postives = 227/591 (38.41%), Query Frame = 2
Query: 4613 KKCNCNGY-----SKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPC-----------------------------QCPSAHYNF-------AQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEK----KTCQSCYSG---CTKDLLVRIDTLNE-----TVINFKWKTL------------------PSTLDLRLNK----------INISIQMSSELLNSINKFYKLKNEIKNFSIQFQ---KMKDDAKEKYGEIEKL--------KTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITL-------AASVLK-DVLKYVDELNQIIKYTQ-SHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAV 6064
K C C+G CD+ TG+C C+ N G C LC GFY + C+ C +C HY F A +C +S + C C+ G +G+ C+ C + G C C+CNQ S+ CN G CQC PGV+G +CD C +V+ K + C +C+ T + +ID++ E T+ F + L P+++DL +K + + Q++ L N+ + + + N ++ + K D AKE +E L T+ + LE + IN G S EL N LE + +Y+E+ + L + + LK D+ ++ D L + +++ S +S + + K + +T +++ L K LIN + L +I +K+ DNLR + +L++ +K+ +E + VRE H + +L D ++ + K+ +L + T A +A AYS I + A
Sbjct: 1915 KPCECSGNINPEDQGSCDTRTGECLRCLNNTFGAACNLCAPGFYGDAIKLKNCQSCDCDDLGTQTCDPFVGVCTCHENVIGDRCDRCKPDHYGFESGVGCRACDCGAASNSTQCDPHTGHCACKSGVTGRQCDRCAVDHWKYEKDG--CTPCNCNQGYSRGFGCNPNTGKCQCLPGVIGDRCDACPNRWVLIKDEGCQECNNCHHALLDVTDRMRYQIDSVLEDFNSVTLAFFTSQKLNYYDQLADELEPKVKLLDPNSVDLSPSKKANSELESDAKSYAKQVNQTLANAFDIRERSSTTLGNITVAYDEAVKSADQAKEAIASVEALSKNLEAAASTKIDAALEQAQHILGQIN---------GTSIELTPNEQV-LEKARKLYEEVNTLVLPIKAQNKSLNALKNDIGEFSDHLEDLFNWSEASQAKSADVERRNVANQKAFDNSKFDTVSEQKLQAEKNIKDAGNF---LINGDLT-LNQINQKL----DNLRDALNELNSFNKNVDEE-----LPVRE---------DQHKEADALT--DQAEQKAAELAIKAQDLAAQYTDMTASAEPAIKAATAYSGIVEAVEAA 2469 HSP 12 Score: 84.7297 bits (208), Expect = 2.092e-15 Identity = 76/259 (29.34%), Postives = 103/259 (39.77%), Query Frame = 2
Query: 3137 KCKCGSNVINRICDKCENLHYWNNRTD----CVPCNCGRGSLNPKCDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTGCK------KCPPCYNG---------QVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIG--CKKCNCY--INGSYSNICNKISGICA-CKTAFFGSKCDRCNEGYYG----FPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEG-GCFSCFCFGVS 3823
+C C C+ C +Y + C+PC C S CD TG C KC C +C GY+G G CP ++ ++ C C P G C C+ + E+IG CK C C IN C+ +G C C FG+ C+ C G+YG NCQ C C + GT++ CD G C C +NVIG +C C+ + G G GC +C C S
Sbjct: 1775 RCSCPPGYSGHSCEDCAPGYYRDPSGPYGGYCIPCECNGHS--ETCDCATGICSKCQHGTEGDHCERCVSGYYGNATNGTPGDCMICACPLPFDSNNFATSCEISESGDQIHCECKPGYTGPRCESCANGFYGEPESIGQVCKPCECSGNINPEDQGSCDTRTGECLRCLNNTFGAACNLCAPGFYGDAIKLKNCQSCDCDDLGTQT-----CDPFVGVCTCHENVIGDRCDRCKPDHY---GFESGVGCRACDCGAAS 2023 HSP 13 Score: 72.4034 bits (176), Expect = 1.044e-11 Identity = 68/234 (29.06%), Postives = 88/234 (37.61%), Query Frame = 2
Query: 1001 ISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDL-CKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNL 1696
++ +C C N G NCE C + P C C+C+ + E NK G CI C+E G C++C+ GFY YP C PC C G+ N G C C + G C LC+ GY YP +CLPC C GS S C C N D C +CK G++N S C+P C S+ L
Sbjct: 509 VTTGECKCLTNFGGDNCERCKHGYFNYP-----TCSYCDCDNQGTESEI----------CNKQS-----GQCI-CREGFGGPRCDQCLPGFYN---------YPDCKPCNCSSTGSSAITCDN--------TGKCNCLNNFAGKQCTLCTAGYYSYP--DCLPCHCDSHGSQGVS---CNSDGQCLCQPNFDGRQCDSCKEGFYNFPSCEDCNCDPAGVIDKFAGCGSVPVGEL 699 HSP 14 Score: 70.8626 bits (172), Expect = 2.647e-11 Identity = 65/222 (29.28%), Postives = 93/222 (41.89%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPP-----CNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCF 1654
+C C+ G C+ C P F P C+ CNC+ + + ++ + G+ NC N G C C G+Y YP C PC C G+ G SC S+G+ C+C+ ++G CD C G+ +P+ C C C G V F C C CK V + +C CK Y+NL+ +N EGCE C C+
Sbjct: 558 GQCICREGFGGPRCDQCLPGFYNYP-----DCKPCNCS----------STGSSAITCDNTGK-------CNCLNNFAGKQCTLCTAGYYS---------YPDCLPCHCDSHGSQGVSCNSDGQ---------CLCQPNFDGRQCDSCKEGFYNFPS--CEDCNCDPAG-VIDKFAGCGSVPVGELCKCKERV-TGRICNECKPLYWNLNISNTEGCEICDCW 735 HSP 15 Score: 70.4774 bits (171), Expect = 3.670e-11 Identity = 45/134 (33.58%), Postives = 60/134 (44.78%), Query Frame = 2
Query: 4637 SKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
S++C+ +G C C G +C C GFY NY C+PC C S + I KC C ++GK C C G+Y P C C C+ GSQ ++CN G C C P G +CD C +
Sbjct: 550 SEICNKQSGQCI-CREGFGGPRCDQCLPGFY----NYPDCKPCNCSST------GSSAITCDNTGKCNCLNNFAGKQCTLCTAGYYSYP----DCLPCHCDSHGSQGVSCNSDGQCLCQPNFDGRQCDSCKEGF 668 HSP 16 Score: 68.5514 bits (166), Expect = 1.334e-10 Identity = 42/114 (36.84%), Postives = 53/114 (46.49%), Query Frame = 2
Query: 3473 DFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEG-GCFSCFC 3811
D+N G CNC GS S C+ G C CK C C YYGFP+C+PC C + C+ TG+C C NVIG C+ C T+ G ++ GC C C
Sbjct: 1367 DYNS--GALPCNCDYAGSTSFECHPFGGQCQCKPNVIERTCGACRSRYYGFPDCKPCKCPNSAM-------CEPTTGECMCPPNVIGDLCEKCAPNTY---GFHQVIGCEECAC 1468 HSP 17 Score: 67.0106 bits (162), Expect = 3.933e-10 Identity = 57/195 (29.23%), Postives = 86/195 (44.10%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCN--GYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQG--SQSLNCNEFGI---CQCSPGVLGIKCDQCAPNY 5038
+CIC G C + G+Y N P+ K CNC+ G S + TG C NC+ N G +C LC G+Y +Y C PC C S H + +C + +C C+ + G+ C++C++GFY P C+ C+C+ G + C + C+C V G C++C P Y
Sbjct: 559 QCICREGFGGPRCDQCLPGFY----NYPD----------------CKPCNCSSTGSSAITCDNTGKC-NCLNNFAGKQCTLCTAGYY----SYPDCLPCHCDS-HGSQGVSCNS-----DGQCLCQPNFDGRQCDSCKEGFYNFP----SCEDCNCDPAGVIDKFAGCGSVPVGELCKCKERVTGRICNECKPLY 718 HSP 18 Score: 67.0106 bits (162), Expect = 4.809e-10 Identity = 52/169 (30.77%), Postives = 70/169 (41.42%), Query Frame = 2
Query: 4604 INLKKCNCNGYSKVCD------------------SVTGDCQNCMGNREGNKCQLCREGFYKNDKNY----DQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFG-ICQCSPGVLGIKCDQCAPNY 5038
N + CNC+G+S C G CQNC N G C C+ +Y+ + D C PCQC Y F+ E+ C+CR + SC++C G+YG P C+ C+CN G+ +C E G C C G C QCA Y
Sbjct: 328 FNCEPCNCHGHSNECKYDEEVNRKGLSLDIHGHYDGGGVCQNCQHNTVGINCNKCKPKYYRPKGKHWNETDVCSPCQC---DYFFSTGHCEEETG---NCECRAAFQPPSCDSCAYGYYGYP----NCRECECNLNGTNGYHCEAESGQQCPCKINFAGAYCKQCAEGY 486 HSP 19 Score: 61.2326 bits (147), Expect = 2.309e-8 Identity = 65/222 (29.28%), Postives = 93/222 (41.89%), Query Frame = 2
Query: 1004 SVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRK---SNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCK--PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
S +C CK N G+ C+ C + P C+ C CN+ +N+C+ G C C N G NCE+C G++ YP C+ C C GT +I +++ G CIC++G+ G CD C G+ YP +C PC CS GS S C CNC ++ + C C GY++ C PC+C
Sbjct: 464 SGQQCPCKINFAGAYCKQCAEGYYGFPE-----CKACECNKIGSITNDCNVTT------------------GEC-KCLTNFGGDNCERCKHGYFN---------YPTCSYCDCDNQGT-------ESEICNKQSGQCICREGFGGPRCDQCLPGFYNYP--DCKPCNCSSTGS---SAITCDNTGKCNC-LNNFAGKQCTLCTAGYYSYPD-----CLPCHC 634 HSP 20 Score: 55.0694 bits (131), Expect = 1.849e-6 Identity = 88/335 (26.27%), Postives = 111/335 (33.13%), Query Frame = 2
Query: 914 YYSLRNIEIGGKCI---CNNHADKCDKVIENGISVAKCNCKHNTCGSNCETC--------------------CPL-FNQ--------------------RPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGG-----GVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGT------IGSCVSNGEDIGDQ----KP-----------------------------GDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKP---PCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPC 1645
YY + GG CI CN H++ CD GI C+H T G +CE C CPL F+ +P CE C S Q + S N +G G C+ C NT G C C GFY A +L C C C LGT +G C + IGD+ KP G C CK G G CD C++ + +Y C PC C+ G R + C P C C V D C C N + + EGC+ C
Sbjct: 1794 YYRDPSGPYGGYCIPCECNGHSETCD--CATGIC---SKCQHGTEGDHCERCVSGYYGNATNGTPGDCMICACPLPFDSNNFATSCEISESGDQIHCECKPGYTGPRCESCANGFYGEPESIGQVCKPCECSGNINPEDQGSCDTRTGECLRCLNNTFGAACNLCAPGFYGD----AIKLKNCQSCDCDDLGTQTCDPFVGVCTCHENVIGDRCDRCKPDHYGFESGVGCRACDCGAASNSTQCDPHTGHCACKSGVTGRQCDRCAVDHWKYEKDGCTPCNCN-QGYSRG--FGCNPNTGKCQCLPGVIG-DRCDACPNRWVLIKD---EGCQEC 2112
BLAST of Laminin subunit alpha-2 vs. Ensembl Zebrafish
Match: lama1 (laminin, alpha 1 [Source:ZFIN;Acc:ZDB-GENE-060118-1]) HSP 1 Score: 694.115 bits (1790), Expect = 0.000e+0 Identity = 425/1222 (34.78%), Postives = 650/1222 (53.19%), Query Frame = 2
Query: 86 LKMRIFIIFILLIVH------KAHSQDDEPEILSVNVDGLLMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT----HKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKC--DKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSN----GEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNP---TVYYWTAPPPYLGNWITSYGTIFRYVVKIEL-QSTLKGSLIDSPDVIIKCGDFRLFHSVS---IEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYY-SNQKSIELLNITLSRGEVHLALDEMP-HIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP--RLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYL--KDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR-TDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGL-DVTGCKKCPPCYNGQVCN---------NEGICVCPPNTIGNLCNKCSEDSWDFNE--TIGCKKCNCYINGSYSNICNKISGI 3559
++MR+ ++++L +V +A + P IL+ L NA+I+++ATCG PE+FC+LV+ + +R+ NP C +CD ++N ++HPI A D NLWWQSPS+ G ++ V VT+DL+QV+Q+ YI++K +SPRPGNWILERSLDG + PW F+A + C YN+ R P P+ DDE+ CT+YYS+ L GEI SLINGR S + L+ F SAR+IR R + TL ADLMT ++ + RYYYS+++I +GG CIC HA C D V + +C C+HNTCG +C CCP ++Q PW+P + CE+CNC+ K+++C YNQTVA L+LS+N G+ GGGVC+NC+ NT G+NCE C G+YRP V PC CQC G++ C+ + D G PG C+CK+G+ G CD C+ G+ +P C C CS+DGS T C C CK +V C CK G++NL ++NPEGC C+CFG+++ C S + + + G ++ + + ++ EN I R + +V+ W AP P+L N +TSYG+ Y V + + + +L VII+ L + S + + ++VF+ + P + ++ ++E ++ L V L ++A+ S + ++ L +++L G+ + + ++ +E C CP G++G SCE C G+ RV +L+ G C C C +H+ CD +NGEC C HNT G HC+ C G+YGDA + C++C CP + C + ++C +C +G C RC +GY+G P C C+CN N + CD TGEC +C +T G C+ C+ G+YGDA+ K+C+ C C S GS S C+ +TG+C+C+ N V + CD C G + + C PC C G +S+ C+ G+C C+ V GD+C++C GY+N+ C C C+ +C+ G+C C + C++C+ H+ ++ + C CNC GS + +CD+ +G C CA + C +C +GY + C P Q C+ + G C C N G C++C S+ + GC C C+ S++C ++SG+
Sbjct: 2 MEMRMKMLWLLALVFSSVICVEAQQRGLFPAILN------LASNAEISTNATCGDPDPEMFCKLVEHVPGRRIRNPQ----CRICDANSQNPKEQHPITNAI-DGTNLWWQSPSIKNGRQFHWVTVTLDLRQVFQVAYIIIKAANSPRPGNWILERSLDGVNFQPWQFYAISDTECLTRYNITPRIGP---------PTYKRDDEVICTSYYSRLVPLEHGEIHTSLINGRPSA--------DDLTPELLEFTSARFIRLRLQRIRTLNADLMTLSYRDPKDVDPIVTRRYYYSIKDISVGGMCICYGHAQSCPWDPVTKK----LQCVCEHNTCGESCNECCPGYHQEPWQPGTLSDGNTCEKCNCHNKADDCFYNQTVADLKLSMNTHGQFTGGGVCVNCRHNTAGVNCETCADGYYRPHQVSPYAEEPCVECQCDMRGSVSPVCIRDDNHANPDAG-LSPGQCVCKEGFAGEKCDRCAFGFRDFP--VCSRCECSLDGSHNTD--PCM-ECVCKANVMGSH-CDLCKQGFYNLQASNPEGCTECFCFGVSDVCESSTWFSSSVVHRDG------VLHRLHQTSISFWSPVSDENLIISNRSTDADPSSVWTWAAPEPFLNNKLTSYGSFLNYSVAYDTSEENVDKTLRSHFSVIIEGNGRTLRQAQSARLLLNAHTHQRVFVQMLPQIFIDSH--SGRSVQRDELMTVLADVAALRVRAELEDSAEGTLRLSHVSLGVGDSNSDVSKISLDVEHCECPWGYSGTSCELCIPGFYRVGGILFGGNCLPCECNDHATECD-INGECLGCAHNTTGPHCDQCLPGFYGDASEGTPDDCQRCSCPLTLASNNFSPTCQLQAPGEFICDQCQPGYTGDKCQRCADGYFGEPAVPGQRCSPCECNGNVDPSEAGRCDTHTGECLKCVGHTAGPHCERCRDGYYGDAIQEKNCQACGCYSNGSLSTICNLITGQCECKHNVVGKTCDRCQEGYHGINSGEGCRPCECNQSGALSAS--CD-EEGRCQCITGVTGDKCDRCHHGYYNFKENGCTACDCA--HTHGNCNDQTGECICPPHTHGLKCEQCDEGHWGHDGVSGCKVCNCSAVGSSSSQCDLSSGQCVCALQFSGLTCDRCALGYRNFPQCSACDCNPNGTRAQFCDEALGVCGCEDHGQCSCKDNVGGRGCDECKSGSFGLSALNPAGCSPCFCF---GLSDVCEELSGL 1167 HSP 2 Score: 357.066 bits (915), Expect = 7.408e-98 Identity = 345/1238 (27.87%), Postives = 537/1238 (43.38%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYY--GNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDA---LTKSCKKCDCD-SLGST--SENCD-KLTGK--CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLC-SPHALSADCDLNNGKCKCGSNVINRICDKC-ENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSN----ELTKCD-KITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCH---AILNHRIVENS----IYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHS-KNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIK-IKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINL-KKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY-KNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCT--KDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVL-KYVDELNQ--IIKYTQSHIQSDQINNLKILKNKTIMA------------DEINT---NNKEYLNTMSTKKKEHELYIILINER--------------MKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSS-NHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTK--DFNFLHNAEQAKEAYS 6037
+C SG C+ CI G+Y G +C C+CN++ CD + GEC C++NT G C C GFYGDA C++C C +L S S C + G+ CD C+ + +C C G + + ++C PC C S C+T +G+C C+ + G CE+C +GY+ NC+ C C S +LS C+L G+C+C NV+ + CD+C E H N+ C PC C + G+L+ CD E G C+C V C +C GY+ GC C + CN++ G C+CPP+T G C +C E W + GCK CNC GS S+ C+ SG C C F G CDRC GY FP C C C NGT++ L C + GQC CK NV G C C+ G+F GC CFCFG+S+ C ++ IV S + D + +LE G YS + + + L + P Y + + +N + L + + ++ D G + Q + G + ++ S N I EH+ KW +Y N + D+ + D FM L N+ + +K Y + I I + T + G VE C C G CQ GYY++ + N M+ NR LI C C+ +S+ CD TG C C N G C +C G+Y K + C C CP +F+ C +E + + +C +C GY G+ CE C G++GNP + G C+ C C++ GS C+ G C+C PGV G CDQC +V+ + C SC CT + ++ T +N L L ++IQ + L S+ + N+ ++ Q ++ ++ G+I L+ + V ED + + +QL LE I NI+ + + S + L + +DE N +++ T ++ + NL +N + DEI+ NN+ +ST H L + + E ++D+Q +T R+N+ +++ Q+ +DL++ + ED+ ++ + L + + +L+KHV+ +L+ LTK ++ AE +A S
Sbjct: 713 ECPWGYSGTSCELCIPGFYRVGGILFGGNCLPCECNDHATECD-INGECLGCAHNTTGPHCDQCLPGFYGDASEGTPDDCQRCSCPLTLASNNFSPTCQLQAPGEFICDQCQPGYTGDKCQRCADGYFGEPAVPGQRCSPCECNGNVDPSEAGRCDTHTGECLKCVGHTAGPHCERCRDGYYGDAIQEKNCQACGCYSNGSLSTICNLITGQCECKHNVVGKTCDRCQEGYHGINSGEGCRPCECNQSGALSASCD-EEGRCQCITGVTGDKCDRCHHGYYNFKENGCTACDCAHTHGNCNDQTGECICPPHTHGLKCEQCDEGHWGHDGVSGCKVCNCSAVGSSSSQCDLSSGQCVCALQFSGLTCDRCALGYRNFPQCSACDCNPNGTRAQFCDEALGVCGCEDHGQCSCKDNVGGRGCDECKSGSFGLSALNPAGCSPCFCFGLSDVCEELSGLVRAPIVLGSSPALLRVVSQSDLQGTLE-GVYYSDDEMLLDVDQLNKLSL-LTGPYYWRLPQKYNGNKLLSYGGRLSYTLTFFAQD---GVGLANQEPQVL--LRGGHLRKLVIYTDASAPANGIRSTQEHALTEHKW---KYFNSVSDEAVSHAD------------FMSVLSNLEYVIIKASYGSGLQQSRISNISMETALEADELPEGGDVARLVEICECPPGYAGLSCQECAPGYYRQAVSELN------MKGRNRPLIQPCVPCRCSNHSQSCDLHTGQCLGCQHNTAGEHCHVCAAGYYGKVQGSVSDCSLCTCPLRGQSFSSTCV-LEGAGDFRCDRCEEGYEGRYCERCAVGYFGNPSEPGGRCQVCACSESGSVHSVCDAHTGRCECKPGVRGHLCDQCEERHVLVHEQCVSCNDSCTGVLLDDLDALDVSITSVNLTGVILAPYSQL------MTIQNQTRELTSLMSW----NQTPDY--QLTAGEEHSRNLSGQITDLQQQVQSVFEDSGDVLLTSEQRFTQGKQL-------------LELINNIHSAIHGLVEQVSSVNVTLDEGLDEANSTFLLEETSETLEKIRSFNLTHCRNAAEIQLSSAVSVLEKVEDEISKPYRNNQIRRENISTTLNTHNLNLQQVQESLNTAKTHNNQSRRLLQDIQSNTHTLTISRNNVSSLNHEVET---QVQEAQDLLTDALNIAEDMDNGTTTLDELKNDLELWSPSLRKHVD-------SLVMDLTKRDALQLVYRAEDHAQALS 1884 HSP 3 Score: 236.498 bits (602), Expect = 2.408e-61 Identity = 165/556 (29.68%), Postives = 250/556 (44.96%), Query Frame = 2
Query: 1208 GVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPP-----------CNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYID-ERFNNPTV----YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR---LFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH-------IEKCNCPPGFNGLSCESCESGYRR--VNNVLYQGQ-------CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS-CKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLCK 2758
G CI C +T G+ CE+C +G + V C C C +G+ S G C+C + G+ CD C++GY +P +C C C+ +G+ R F C C+CK +V C CK+G F L + NP GC PC+CFG+++ C LS L + L Y + + +D ++ N ++ YYW P Y GN + SYG Y + Q + G P V+++ G R ++ S ++ + +WK D+A + +F+S L ++ ++IKA Y S + + NI++ E L DE+P +E C CPPG+ GLSC+ C GY R V+ + +G+ C C C NHS +CD G+C C HNTAG HC CA+GYYG + +++ C C CP + C + ++ C +C G +C+RC GY+GNP + C+ C C+ + CD TG C C G C C+
Sbjct: 1015 GECI-CPPHTHGLKCEQCDEGHWGHDGVSG-----CKVCNCSAVGSSSSQCD-------LSSGQCVCALQFSGLTCDRCALGYRNFP--QCSACDCNPNGT-RAQF--CDEALGVCGCEDHGQCSCKDNVGGRG-CDECKSGSFGLSALNPAGCSPCFCFGLSDVCEELSGLVRAPIVLGSSPALLRVVSQSDLQGTLEGVYYSDDEMLLDVDQLNKLSLLTGPYYWRLPQKYNGNKLLSYGGRLSYTLTFFAQDGV-GLANQEPQVLLRGGHLRKLVIYTDASAPANGIRSTQEHALTEHKWKYFNSVSDEAVSHADFMSVLSNLEYVIIKASYGSGLQQSRISNISM---ETALEADELPEGGDVARLVEICECPPGYAGLSCQECAPGYYRQAVSELNMKGRNRPLIQPCVPCRCSNHSQSCDLHTGQCLGCQHNTAGEHCHVCAAGYYGKVQG-SVSDCSLCTCPLRGQSFSSTCVLEGAGDFRCDRCEEGYEGRYCERCAVGYFGNPSEPGGRCQVCACSESGSVHSVCDAHTGRCECKPGVRGHLCDQCE 1546 HSP 4 Score: 167.162 bits (422), Expect = 2.999e-40 Identity = 158/606 (26.07%), Postives = 259/606 (42.74%), Query Frame = 2
Query: 3407 GICV-CPPNTIGNLCNKCSEDSWDFNETIG-----CKKCNCYINGSYSNIC-------NKISGI----CACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI------------LNHRIVENSIYEYHIFDEKNSLESGK----DYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSV--DCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPS--AHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQ--QGSQSLNCNEF-GIC-QCSPGVLGIKCDQCAPNY---VVEKKTCQSCYSGC 5080
G+CV C NT G C C++ + ++ C +C C + GS S +C N +G+ C CK F G KCDRC G+ FP C C C+ +G+ + + +C CK NV+G+ C +C++G + + GC CFCFGVS+ C + + HR+ + SI + ++N + S + D S++ W P N + + S F+N SV D + + + V +E NG ++ + + + N H+ +V +L + I S ++ RD M L +V +L+V+ + +++ ++ V ++ + K VE C C G C+ I G+Y+ G + G L C CN ++ CD + G+C C N G C C GFY + + D C+ C CP A NF+ C ++++ E C QC+ GY+G C+ C G++G P + G C C+CN S++ C+ G C +C G C++C Y +++K CQ+C GC
Sbjct: 366 GVCVNCRHNTAGVNCETCADGYYRPHQVSPYAEEPCVECQCDMRGSVSPVCIRDDNHANPDAGLSPGQCVCKEGFAGEKCDRCAFGFRDFPVCSRCECSLDGSHNTDPCM------ECVCKANVMGSHCDLCKQGFYNLQASNPEGCTECFCFGVSDVCESSTWFSSSVVHRDGVLHRLHQTSISFWSPVSDENLIISNRSTDADPSSV-----------WTWAAPEPFLNNKLTSYGS----------FLNYSVAYDTSEENVDKTLRSHFSVIIEG-NG---------RTLRQAQSARLLLNAHTHQRVFV------QMLPQIFIDSHSG---RSVQRDELMTVLADVAALRVRAELEDSAEGTLRLSHVSLGVGDSNSDVSKISLDVEHCECPWGYSGTSCELCIPGFYR----------VGGILFGGNCL----PCECNDHATECD-INGECLGCAHNTTGPHCDQCLPGFYGDASEGTPDDCQRCSCPLTLASNNFSPTC-QLQAPGEFICDQCQPGYTGDKCQRCADGYFGEPAVPGQRCSPCECNGNVDPSEAGRCDTHTGECLKCVGHTAGPHCERCRDGYYGDAIQEKNCQAC--GC 907 HSP 5 Score: 59.3066 bits (142), Expect = 1.117e-7 Identity = 58/223 (26.01%), Postives = 93/223 (41.70%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKNDK----NYDQCEPCQCPSAHYNFAQNCTRIESSLEL-------KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLN-CNEFGICQCSPGVLGIKCDQCAPNY 5038
VT +C+C T G C GY++E + + G++ GN +KCNC+ + C +V G C NC N G C+ C +G+Y+ + + C CQC + + C R ++ +C C+ G++G+ C+ C GF P+ C C+C+ GS + + C E C C V+G CD C +
Sbjct: 291 VTKKLQCVCEHNTCGESCNECCPGYHQEPW------QPGTLSDGN----TCEKCNCHNKADDCFYNQTVADLKLSMNTHGQFTGGGVCVNCRHNTAGVNCETCADGYYRPHQVSPYAEEPCVECQC-DMRGSVSPVCIRDDNHANPDAGLSPGQCVCKEGFAGEKCDRCAFGFRDFPV----CSRCECSLDGSHNTDPCME---CVCKANVMGSHCDLCKQGF 495 HSP 6 Score: 55.8398 bits (133), Expect = 1.198e-6 Identity = 47/168 (27.98%), Postives = 68/168 (40.48%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKC-NCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGG--VCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLC 1444
C C+NH+ CD + +C C+HNT G +C C + + V+ C C C + S++ T +EG G C C+E EG CE+C G++ + C C C G++ S + D G C CK G G CD C
Sbjct: 1407 CRCSNHSQSCD------LHTGQCLGCQHNTAGEHCHVCAAGYYGKVQGSVSDCSLCTCPLRGQ--SFSSTCV-----------LEGAGDFRCDRCEEGYEGRYCERCAVGYFGNPSEPGGR---CQVCACSESGSVHS-------VCDAHTGRCECKPGVRGHLCDQC 1545
BLAST of Laminin subunit alpha-2 vs. Ensembl Zebrafish
Match: CABZ01065423.1 (pep chromosome:GRCz11:20:1834262:1891596:1 gene:ENSDARG00000112019.1 transcript:ENSDART00000185316.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01065423.1) HSP 1 Score: 456.062 bits (1172), Expect = 2.314e-140 Identity = 252/577 (43.67%), Postives = 332/577 (57.54%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGY----LEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVA-----VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSN-GEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKI--ALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLG 1864
L A+I ++ATCG GPE++C+LV+ + + + NP C CD + + +RHPI+FA D N WWQSPS+ G+ +V VT+DL+QV+QI Y++LK +SPRPGNWILERSLDGE +MPW ++A T C YN+ R P PS DDE+ CT++YS+ L +GEI SLINGR S + TL+NF SAR+IR +F + TL ADLMT + ++ + RYYYS+++I +GG CIC HA C + C C+HNTCG +C+ CCP +NQ+PW VCE+CNC+ KS EC YNQTVA +LS+N G EGGGVC+ C ENT GINC+ CI G+YRP V E +PCTPC C G + S C+ + + D G CICK GY G CD C GY +P +C C CS DGSV C+PPC CK HV D C CK G+++L GC+ CYC G+ + C+ ++D+ GW LT GE + A P + +T S P YYW+AP YLG
Sbjct: 23 LASMAEIKTNATCGETGPEMYCKLVEHVPGRPVKNPQ----CRTCDLNSDYDYERHPIKFAI-DGTNRWWQSPSIMNGMDLHHVTVTLDLQQVFQIAYVILKAANSPRPGNWILERSLDGETFMPWQYYAITDTECITRYNIIPRTGP---------PSYTHDDEVICTSFYSKIHPLENGEIHTSLINGRPSADDPS--------PTLLNFTSARFIRLQFQRIRTLNADLMTLALHDPRDIDPIVTRRYYYSIKDISVGGMCICYGHAKACP--LNTHTKKFSCECEHNTCGESCDRCCPAYNQKPWMAGTFLTRHVCEKCNCHNKSEECYYNQTVADAKLSLNIHGEYEGGGVCLGCSENTGGINCQSCIDGYYRPSGVSPLEPWPCTPCTCDLRGALHSTCIPDESQATTDLPVGSCICKPGYAGERCDRCVFGYAGFP--QCERCRCSSDGSVNQD--PCQPPCVCKEHVEGRD-CDRCKQGFYHLSGGRARGCDKCYCSGVASLCAESQWDYTNVTDMSGWYLTGADGEGLVWAEPSRETAHQVTVSQSDAQTHLTAP--YYWSAPSAYLG 568 HSP 2 Score: 74.7146 bits (182), Expect = 1.418e-12 Identity = 74/265 (27.92%), Postives = 107/265 (40.38%), Query Frame = 2
Query: 2138 LIKAKYYSNQKSIELLNITLSRGEVHL-ALDEMPHIEKCNCPPGFNGLSCESCESGYRR----VNNVLYQGQCEACACYNHSNTCD----------SLN--------GECQNCTHNTAGAHCEDCASGYYGDAKSKNLN--SCKKCPCPRLDYQMTELC---SSYSSENYVCHKCL--HNTSGVHCDRCIEGYYGNPKKKISCKRCKCNN----NQESCDPVTGECRCSYNTMGTECQLCKHGFY--GDALTKSCKKCDCDSLGS 2824
++ +YY + K I + + + G L+ C C G SC+ C Y + L + CE C C+N S C SLN G C C+ NT G +C+ C GYY + L C C C L + C S ++ + C+ +G CDRC+ GY G P+ C+RC+C++ NQ+ C P C C + G +C CK GFY + C KC C + S
Sbjct: 246 IVTRRYYYSIKDISVGGMCICYGHAKACPLNTHTKKFSCECEHNTCGESCDRCCPAYNQKPWMAGTFLTRHVCEKCNCHNKSEECYYNQTVADAKLSLNIHGEYEGGGVCLGCSENTGGINCQSCIDGYYRPSGVSPLEPWPCTPCTC-DLRGALHSTCIPDESQATTDLPVGSCICKPGYAGERCDRCVFGYAGFPQ----CERCRCSSDGSVNQDPCQP---PCVCKEHVEGRDCDRCKQGFYHLSGGRARGCDKCYCSGVAS 502 HSP 3 Score: 74.7146 bits (182), Expect = 1.455e-12 Identity = 49/157 (31.21%), Postives = 72/157 (45.86%), Query Frame = 2
Query: 3407 GICV-CPPNTIGNLCNKCSEDSWDFN-----ETIGCKKCNCYINGSYSNIC---------NKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
G+C+ C NT G C C + + + E C C C + G+ + C + G C CK + G +CDRC GY GFP C+ C C+ +G+ + D C CK++V G C C++G + G GC C+C GV+ C
Sbjct: 353 GVCLGCSENTGGINCQSCIDGYYRPSGVSPLEPWPCTPCTCDLRGALHSTCIPDESQATTDLPVGSCICKPGYAGERCDRCVFGYAGFPQCERCRCSSDGSVNQ-----DPCQPPCVCKEHVEGRDCDRCKQGFYHLSGGRARGCDKCYCSGVASLC 504 HSP 4 Score: 73.9442 bits (180), Expect = 2.492e-12 Identity = 63/229 (27.51%), Postives = 90/229 (39.30%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPK------KKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY-------LKDKKCIPCNCEIQGRI-------SSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY 3199
+C HNT G CDRC Y P + C++C C+N E EC + + L HG Y G C + TG +C+ SC G Y L+ C PC C+++G + S+ + G C C G+RC++CV GY +P C++C CS S + D C C +V R CD+C+ Y
Sbjct: 285 ECEHNTCGESCDRCCPAYNQKPWMAGTFLTRHVCEKCNCHNKSE-------ECYYNQTVADAKLSLNIHGEYEG--------------GGVCLGCSENTGGINCQ---------SCIDGYYRPSGVSPLEPWPCTPCTCDLRGALHSTCIPDESQATTDLPVGSCICKPGYAGERCDRCVFGYAGFPQCERCRCSSDG-SVNQDPCQPPCVCKEHVEGRDCDRCKQGFY 482
BLAST of Laminin subunit alpha-2 vs. Ensembl Zebrafish
Match: lamc1 (laminin, gamma 1 [Source:NCBI gene;Acc:286832]) HSP 1 Score: 427.557 bits (1098), Expect = 6.914e-122 Identity = 329/1099 (29.94%), Postives = 499/1099 (45.40%), Query Frame = 2
Query: 92 MRIFIIFILLIVHKAHS---------QDDEPE-ILSVNVDGLLMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAKSDDDNL---WWQSPSMAQGLKYQN-VNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNL-KIS-----DLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR-----LFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKE-EFISCLVKVDKLLIKAKYYSNQKSIELLNITL---SRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQ-GQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSEN-YVCHKCLHNTSGVHCDRCIEGYYGNPKKK----ISCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDA----LTKSCKKCDCDSLGSTSEN--CDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRT 3214
M +F +L + A S +DD P+ + V+ NA + ++ TCG+ PE FC + + C +C+ + H + + + WWQS +M G++Y N +N+T+ L + + I Y+ LK S I +RS + + P+ +++ + Y+ R + + T + + CT +S L G + S + GR S N + + L ++++A IR + T ++ + + + YYY++ + +GG+C CN HA +C ++N S CNCKHNT G++C C P +N RPW+ C CNCN KS EC ++ ++ R G GG C NC +NT+G CE+C+ +YR C C C+ +G++ + N G C CK G G CD C GY C PC C+ GS + + C CK +V+ + C CK GYFNLD NP+GC PC+CF + C S ++ KI+ D +GWK + + + + E S I E + Y+ AP +L N + SYG +I+ + + + DV+++ R + S Q VF T++ P + K +F L + ++I+ YS Q + L N++L RG A +EKC CP G+ G CE C+ G+RR L + CE C C HS+TCD G C NC HNTAG CE C G+YGD+ + + CK CPCP C+ N VC C T+G C+ C +G++G+P + +C+ C CNNN + +C+ +GEC +C YNT G C CK GFYGDA + CK C C G+ C ++TG+C C + +NR C +C G Y K C CNC G + + C+ SGQC C V G CE+C +F + + CK C C P + SA C +G+C C + CD CE +++N T
Sbjct: 1 MSLFSCLLLWTLWAACSHGAMDECIDEDDRPQRCMPEFVNAAF--NATVVATNTCGSP-PEEFCVQTGVTGVTKS--------CHICNAADPRLHHGAVYLTDYNQPVQPTWWQSQTMLAGIQYPNSINLTLHLGKSFDITYVRLKFHTSRPESFAIYKRSSEDGPWTPYQYYSGSCEKTYSKNNRGF----IRTGED-----EQQALCTDEFSDISPLYGGNVAFSTLEGRPSAYNFDNSPV------LQDWVTATDIRVTLNRLNTFGDEV-----FNDPKVLKSYYYAISDFAVGGRCKCNGHASEC---VKNEYSKLVCNCKHNTEGADCNVCKPFYNDRPWRRATAENPNECLPCNCNGKSAECYFDP----------ELYRATGHGGHCRNCADNTDGPKCERCLANYYR-----EASGQRCLSCGCNPVGSLSTQCDN--------TGRCSCKPGVMGDKCDRCQPGYHSLSEAGCRPCSCNPAGSTQECDVQTGR-CQCKENVDGFN-CDRCKLGYFNLDPQNPQGCTPCFCFQHSTVCESADGYSVHKITSTFDRDDEGWKGKQRDDSSVPV----QWSPSSGEISLISEDY---FPIYFVAPDKFLHNQLLSYGQNLTLNFRIQRHD----ARLSAEDVVLEGSGLRVAVPLIAQGNSYPGEETQTFVFRLHDTTDY------PWRPTIKHADFQKLLYNLTSIMIRGT-YSAQSAGYLDNVSLVTARRGPGTPAR----WVEKCTCPQGYLGQHCEQCDQGFRRSRPELRRFSTCERCNCNGHSDTCDPETGMC-NCQHNTAGLSCERCKDGFYGDSTVGSSSDCKACPCP-----AGATCAVVPKTNEVVCTNCPTGTTGKRCELCDDGFFGDPLGEKGPVRACRACSCNNNIDPNAVGNCNRESGECLKCIYNTAGVFCDRCKQGFYGDARAANVADKCKPCKCSPYGTVDRQTACSQVTGQCPCLPHVINRDCGACELGFYNLQSGKGCERCNCNPIGSTNGQ--CDIVSGQCECQPGVTGQHCERCEVNFFGFSSSGCKPCDCDPEGSESAQCK-EDGRCHCRPGFVGSRCDMCEENYFYNRST 1009 HSP 2 Score: 166.777 bits (421), Expect = 2.390e-40 Identity = 116/346 (33.53%), Postives = 173/346 (50.00%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYYGNP---KKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC--DCRTNFVNRQCDSCTSGLY---LKDK----KCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN-------YPNCKKCLCSPHAL---SADCDLNNGKCKCGSNVINRICDKCENLHYWNNRT--DCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC-NCY 3514
C G HC++C +G+ + ++ +C+RC CN + ++CDP TG C C +NT G C+ CK GFYGD+ S CK C C + G+T K +C T ++C+ C G + L +K C C+C ++ CN SG+C C+ N G C++C +G++ CK C CSP+ C G+C C +VINR C CE L ++N ++ C CNC GS N +CD+ +G C+C V + C +C+V +FG +GCK C P C +G C C P +G+ C+ C E+ + T GC++C NCY
Sbjct: 676 CPQGYLGQHCEQCDQGFRRSRPELRRFSTCERCNCNGHSDTCDPETGMCNCQHNTAGLSCERCKDGFYGDSTVGSSSDCKACPCPA-GATCAVVPKTNEVVCTNCPTGTTGKRCELCDDGFFGDPLGEKGPVRACRACSCNNNIDPNAVGNCNRESGECLKCIYNTAGVFCDRCKQGFYGDARAANVADKCKPCKCSPYGTVDRQTACSQVTGQCPCLPHVINRDCGACE-LGFYNLQSGKGCERCNCNPIGSTNGQCDIVSGQCECQPGVTGQHCERCEVNFFGFSSSGCKPCDCDPEGSESAQCKEDGRCHCRPGFVGSRCDMCEENYFYNRSTPGCQQCPNCY 1019 HSP 3 Score: 162.54 bits (410), Expect = 4.581e-39 Identity = 154/569 (27.07%), Postives = 234/569 (41.12%), Query Frame = 2
Query: 3419 CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTM---FRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQ---YQNDILDKFII-LEDSN--VINSNLTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI--KGI--PCKSCDCNQQGSQSL--NCN-EFGIC-QCSPGVLGIKCDQCAPNYVVEKKT 5056
C NT G C +C + + C C C GS S C+ +G C+CK G KCDRC GY+ C+PCSC G+ +CD TG+CQCK+NV G C C+ G F D GC CFCF S C + + + + + FD + GK + + G + + PIY F + L + +++ +N + D + E +V +E G+R V +I+Q Y + F+ L D+ + F L N+TS+ ++ Y+ + L + ++T + + G VEKC C G HC+ G+ + L R ++CNCNG+S CD TG C NC N G C+ C++GFY + + C+ C CP+ C + + E+ C C G +GK CE C GF+G+P+ KG C++C CN + NCN E G C +C G+ CD+C + + +
Sbjct: 354 CADNTDGPKCERCLANYYREASGQRCLSCGCNPVGSLSTQCDN-TGRCSCKPGVMGDKCDRCQPGYHSLSEAGCRPCSCNPAGSTQ----ECDVQTGRCQCKENVDGFNCDRCKLGYFNLDPQNPQGCTPCFCFQHSTVCESADGYSVHKIT----STFDRDDEGWKGKQRDDSSVPVQWSPSSGEISLISEDYFPIYFVAPDKFLHNQLLSYGQNLTLNFRIQRHDARLSAE-----DVVLEGS----GLRVAVP----------------------LIAQGNSYPGEETQTFVFRLHDTTDYPWRPTIKHADFQKLLYNLTSIMIRGTYSAQSAGYLDNVSLVTAR-----RGPGTPARWVEKCTCPQGYLGQHCEQCDQGFRRSRPEL-------------RRFSTCERCNCNGHSDTCDPETGMC-NCQHNTAGLSCERCKDGFYGDSTVGSSSDCKACPCPA-----GATCAVVPKTNEVVCTNCPTGTTGKRCELCDDGFFGDPLGEKGPVRACRACSCNNNIDPNAVGNCNRESGECLKCIYNTAGVFCDRCKQGFYGDARA 858 HSP 4 Score: 112.464 bits (280), Expect = 9.095e-24 Identity = 94/355 (26.48%), Postives = 150/355 (42.25%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKDKK------CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYF------NYPNCKKCLCSPHALSADCDLNNGKC--KCGSNVINRICDKCENLHYWNNRTD------CVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTG--CKKCPPCY--------NGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC 3805
KC C ++ + C+ C G + C CNC C+ +G C C N G CE+C +G++ + +CK C C A A N C + + C+ C++ + + + C C+C +++P C+ E+G C KC N A C +C G++G KC PC C+ G C C P+ I C C ++ GC++CNC GS + C+ +SG C C+ G C+RC ++GF + C+PC C G++S + K G+C C+ +G++C +C + F + T GC C
Sbjct: 673 KCTCPQGYLGQHCEQCDQGFRRSRPELRRFSTCERCNCN-----GHSDTCDPETGMCNCQHNTAGLSCERCKDGFYGDSTVGSSSDCKACPCPAGATCAVVPKTNEVVCTNCPTGTTGKRCELCDDGFFGDPLGEKGPVRACRACSCN-NNIDPNAVGNCNRESGECLKCIYNTAGVFCDRCKQGFYGDARAANVADKCKPCKCSPYGTVDRQTACSQVTGQCPCLPHVINRDCGACELGFYNLQSGKGCERCNCNPIGSTNGQCDIVSGQCECQPGVTGQHCERCEVNFFGFSSSGCKPCDCDPEGSESAQC----KEDGRCHCRPGFVGSRCDMCEENYFYNRST--PGCQQC 1015 HSP 5 Score: 104.76 bits (260), Expect = 1.598e-21 Identity = 136/499 (27.25%), Postives = 201/499 (40.28%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKT-----CQSCYSGCTK----------DLLVRIDTLNETVINFKWKTLPSTL--------DLRLNKINISIQMSSELL-----------NSINKFYKLKNEIKNFSIQFQKMKDDAKEKYG-------EIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNI-TLAASVLKDVLKY-------VDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKE---YLNTMS---TKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQ 5791
KCI N T G C G+Y G R N + K C C+ Y V C VTG C C+ + C C GFY N ++ CE C C Q C + +C+C+ G +G+ CE C+ F+G G CK CDC+ +GS+S C E G C C PG +G +CD C NY + T C +CYS DL ID+L+ T K L DL L + S ++ LL N N+ +KN + N Q + ++ ++ E++K K +KV + +T D N E+ + +L + A + I+ I K+ + T A ++LK L +DELN+ KY ++ D NL+ K + A+ NK Y N S K E I + DL K+ K K+ ++LR+ ++
Sbjct: 835 KCIYN--TAGVFCDRCKQGFY------------GDARAAN-VADKCKPCKCSPYGTVDRQTACSQVTGQCP-CLPHVINRDCGACELGFY-NLQSGKGCERCNCNPIGSTNGQ-CDIVSG----QCECQPGVTGQHCERCEVNFFGFSSSG--CKPCDCDPEGSESAQCKEDGRCHCRPGFVGSRCDMCEENYFYNRSTPGCQQCPNCYSLVRDKVNQQRQKLLDLQNLIDSLDNTETTVSDKAFEDRLKEAEKTIMDL-LEEAQASKEVDKGLLDRLNNINKTLNNQWNRLQNIKNTVDNTGAQADRARNRVRDAENLINTAREELDKAKEAISKVDIKIPTTSGDPNNMTLLAEE---ARKLSEKHKADADQIEKIAKDANDTSTKAYNMLKKALDGENKTSSDIDELNR--KYLEA---KDLAKNLEKQAAK-VHAEAEEAGNKALKIYANLTSLPPINTKTLEDDANKIKKEASDLDKLIDKTEKEYNDLREDLR 1299 HSP 6 Score: 101.293 bits (251), Expect = 1.774e-20 Identity = 76/255 (29.80%), Postives = 116/255 (45.49%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-----CRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGR---ISSEKFCNTT-SGQCF-CLENVNGDRCEKCVEGYFNYPNCKKCL---CSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY 3382
RCKCN + C V E C C +NT G +C +CK FY D + +T+EN +C+PCNC + E + T G C C +N +G +CE+C+ Y+ + ++CL C+P +LS CD N G+C C V+ CD+C+ ++ + C PC+C +CD++TG C+C +NV +C +C +GYF LD + C PC+
Sbjct: 269 RCKCNGHASEC--VKNEYSKLVCNCKHNTEGADCNVCKP-FYNDRPWRR----------ATAEN-----------------------------PNECLPCNCNGKSAECYFDPELYRATGHGGHCRNCADNTDGPKCERCLANYYREASGQRCLSCGCNPVGSLSTQCD-NTGRCSCKPGVMGDKCDRCQPGYHSLSEAGCRPCSCNPAGSTQECDVQTGRCQCKENVDGFNCDRCKLGYFNLDPQNPQGCTPCF 480 HSP 7 Score: 71.2478 bits (173), Expect = 2.413e-11 Identity = 63/197 (31.98%), Postives = 85/197 (43.15%), Query Frame = 2
Query: 4517 NPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC--DSVTGDCQNCMGNREGNKCQLCREGFYKNDK--------NYDQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY-VVEKKTCQSC 5068
N ++ + E FN P KS + + +C CNG++ C + + NC N EG C +C+ FY ND+ N ++C PC C SA F R C G CE C +Y G C SC CN GS S C+ G C C PGV+G KCD+C P Y + + C+ C
Sbjct: 238 NRLNTFGDEVFNDPKVLKSYYYAISDFAVGG--RCKCNGHASECVKNEYSKLVCNCKHNTEGADCNVCKP-FY-NDRPWRRATAENPNECLPCNCNGKSAECYFDPELYRATGHGGHCRNCADNTDGPKCERCLANYY-REASGQRCLSCGCNPVGSLSTQCDNTGRCSCKPGVMGDKCDRCQPGYHSLSEAGCRPC 429 HSP 8 Score: 65.4698 bits (158), Expect = 1.343e-9 Identity = 55/192 (28.65%), Postives = 81/192 (42.19%), Query Frame = 2
Query: 4619 CNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLD 5164
CNCNG S C G C+NC N +G KC+ C +Y+ + + +C C C + + C +C C+ G G C+ CQ G++ G C+ C CN GS + G CQC V G CD+C Y ++ + Q GCT + T+ E+ + + ST D
Sbjct: 326 CNCNGKSAECYFDPELYRATGHGGHCRNCADNTDGPKCERCLANYYR-EASGQRCLSCGCNPVG-SLSTQCDNTG-----RCSCKPGVMGDKCDRCQPGYHSLSEAG--CRPCSCNPAGSTQECDVQTGRCQCKENVDGFNCDRCKLGYFNLDPQNPQ----GCTPCFCFQHSTVCESADGYSVHKITSTFD 504
BLAST of Laminin subunit alpha-2 vs. Ensembl Zebrafish
Match: lamc3 (laminin, gamma 3 [Source:ZFIN;Acc:ZDB-GENE-060531-121]) HSP 1 Score: 367.081 bits (941), Expect = 2.238e-102 Identity = 294/1010 (29.11%), Postives = 447/1010 (44.26%), Query Frame = 2
Query: 311 CDVCD---NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQN-VNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFG------ITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIK---AKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGY-RRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP----KKKISCKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT------KSCKKCDCDSLGSTSE--NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWN 3205
CD D NRN T + +F +D++ WWQS SM G++Y N VN+T+ L + ++I Y+ LK S RP ++ + + D PW + SC + R + + + L CT +S L G + S + GR S N + + L ++++A I + T + + + + Y+Y++ + +GG+C CN HA +C V E G SV C C+HNT G +C+ C P + RPW C CNC+ ++EC ++ V + GG C+ C+ENT G +CE+C + ++R ++ L PC C C+ LG++ C ++G C+C GV CD+C G+ G C C C GSV C+CK +V C CK G FNL NP+GC C+CFG +NQ + + + + D GWK + +L YL Y +E ++ AP YLGN + SYG EL+ L S+ V ++ + + + + I ++ + + + + EF+ L + L I + Y++Q S L + + D +PH +E C+CP GF G C+ C G+ R + C C C H TC G C CT T G HCE C GYYG+A + + C CPCP T + + +C C GV C+ C +G+YGNP C +C+CN N ++ CD +TG C +C +T G C+ C+ G+YG+AL + CK C C+ G++ +C TG C C + R C C +G + C CNC G +SS C+ +GQC C V G C+ C G+F + + C+ C C P ++ NNG C C + + CDKCE +Y N
Sbjct: 76 CDAFDSELNRNAT--YLTDF-HTDEEPTWWQSQSMFYGVQYPNSVNITLHLGKAFEITYVRLKFYTS-RPESFAIYKRTDEN--GPWQPYQYYSASCRKTYGRDNKGFIRPGDNERTPL------CTDEFSDISPLTGGNVAFSTLEGRPSAYNFDQSLV------LQDWVTATDILISLDRLNTFGDEF-----FKDAKVLRSYFYAISDFSVGGRCKCNGHASEC-VVNEEGRSV--CVCEHNTAGVDCQLCAPFYQDRPWARATAHSANECVRCNCSGLADECVFD---------VEQYRSTGQGGRCLGCRENTAGPHCEQCRENYFR-----SSALQPCQNCNCNALGSVSLQCDADGV---------CVCWPSVSGVKCDICKSGFHSLGPGGCRLCDCDERGSVGVCSAE-DGRCHCKANVEGQS-CNRCKPGSFNLQLENPDGCNKCFCFGHSLACFSSNQYTPIYITSDFLEDPDGWKGVFSRAHEQSLIWKEGEVYLL---PYREENG------FYKAPDKYLGNMLLSYGRTLSMSFTAELEELLPRSVT----VRLEGSGMSVSADLHTQQEPYSQLEKTPINSFSLRLTEKHVNPSVSSFEFLRMLYNLTALHISNAGGQNYTSQLSRVSLESAVQKA---YHPDVLPHALWVEVCSCPAGFAGQFCQLCAPGFTRETPDGGPFSPCVPCNCNQHG-TCHPETGVCA-CTDFTTGRHCERCEDGYYGNALTGSPGDCLPCPCP----DRTTCAQVPETGDVICTNCPTGQRGVRCELCEDGFYGNPLGWGGTVQPCVKCQCNGNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGYYGNALANQLNPERKCKPCACNVAGTSGSPNDCHPNTGNCVCLGHVTGRDCGQCEAGYFNLQPGLGCEMCNCNPIGSLSSA--CHPITGQCMCRTGVEGMSCDTCRMGFFGFSSRGCRACNCDPMGSTSMQCHNNGTCSCRNGFVGYKCDKCEQNYYQN 1010 HSP 2 Score: 124.02 bits (310), Expect = 2.785e-27 Identity = 105/345 (30.43%), Postives = 148/345 (42.90%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY-----GNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP---------NCKKCLCSPHALSA---DCDLNNGKCKCGSNVINRICDKCENLHYWNNRT--DCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC 3505
C +G C C G+ G P C C CN + +C P TG C C+ T G C+ C+ G+YG+ALT S C C C + ++ + C +C T +C+ C G Y + C+ C C ++ C+ +G+C CL + GD CEKC GY+ CK C C+ S DC N G C C +V R C +CE Y+N + C CNC GSL+ C TG C C V C C +G+FG GC+ C P C+N G C C +G C+KC ++ + + C++C
Sbjct: 679 CPAGFAGQFCQLCAPGFTRETPDGGPFSP--CVPCNCNQHG-TCHPETGVCACTDFTTGRHCERCEDGYYGNALTGSPGDCLPCPCPDRTTCAQVPETGDVICTNCPTGQRGVRCELCEDGFYGNPLGWGGTVQPCVKCQCNGNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGYYGNALANQLNPERKCKPCACNVAGTSGSPNDCHPNTGNCVCLGHVTGRDCGQCE-AGYFNLQPGLGCEMCNCNPIGSLSSACHPITGQCMCRTGVEGMSCDTCRMGFFGFSSRGCRACNCDPMGSTSMQCHNNGTCSCRNGFVGYKCDKCEQNYYQNRLSHQCEEC 1019 HSP 3 Score: 117.087 bits (292), Expect = 3.530e-25 Identity = 100/349 (28.65%), Postives = 143/349 (40.97%), Query Frame = 2
Query: 2858 CDCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSADC----DLNNGKCKCGSNVINRICDKCENLHY-----WNNRTD-CVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTGC----KKCPPCYNGQVCN-------------NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTF 3766
C C F + C C G + C+PCNC G C+ +G C C + G CE+C +GY+ +C C C A D+ C G + C+ CE+ Y W CV C C G+++ CD TG C KC + C KC +GY+G + +KC PC CN N G CVC + G C +C ++ +GC+ CNC GS S+ C+ I+G C C+T G CD C G++GF + C+ C+C G+ S +C G C C+ +G KC C + +
Sbjct: 677 CSCPAGFAGQFCQLCAPGFTRETPDGGPFSPCVPCNCNQHGT------CHPETGVCACTDFTTGRHCERCEDGYYGNALTGSPGDCLPCPCPDRTTCAQVPETGDVICTNCPTGQRGVR--CELCEDGFYGNPLGWGGTVQPCVKCQCN-GNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGYYGNALANQLNPERKCKPC----ACNVAGTSGSPNDCHPNTGNCVCLGHVTGRDCGQCEAGYFNLQPGLGCEMCNCNPIGSLSSACHPITGQCMCRTGVEGMSCDTCRMGFFGFSSRGCRACNCDPMGSTS---MQCHN-NGTCSCRNGFVGYKCDKCEQNYY 1008 HSP 4 Score: 114.775 bits (286), Expect = 1.428e-24 Identity = 143/565 (25.31%), Postives = 219/565 (38.76%), Query Frame = 2
Query: 3419 CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY--GFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRP---IYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKV-----KLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIKG----IPCKSCDCNQQGSQSLNCNEFGIC--------QCSPGVLGIKCDQCAPNY 5038
C NT G C +C E+ + + C+ CNC GS S C+ G+C C + G KCD C G++ G C+ C C E G+ + C G+C CK NV G C C+ G+F GC CFCFG S C + + IY F E G +S H + + L +R Y K + LS + R++ M+ + + + +P V +E GM + H++ + SQ + ++ F + +N +++ F+ L N+T+L + + YT + L ++ + + + VE C C A G CQ G+ +E T G CNCN + C TG C C G C+ C +G+Y N + C PC CP C ++ + ++ C C G G CE C+ GFYGNP+ PC C CN +++ N G+C +C G C++C Y
Sbjct: 354 CRENTAGPHCEQCRENYFRSSALQPCQNCNCNALGSVSLQCDA-DGVCVCWPSVSGVKCDICKSGFHSLGPGGCRLCDCDERGS----VGVCSAEDGRCHCKANVEGQSCNRCKPGSFNLQLENPDGCNKCFCFGHSLACFSSNQY----TPIYITSDFLEDPDGWKGV-FSRAHEQSLIWKEGEVYLLPYREENGFYKAPDKYLGNMLLS-YGRTLSMSFTAELEE-----LLPRSVTVRLEGS----GMSVSA--------------DLHTQQEPY--SQLEKTPINSFSLRLTEKHVNPSVSSFEFLRMLYNLTALHISNAGGQNYTSQ----LSRVSLESAVQKAYHPDVLPHALWVEVCSCPAGFAGQFCQLCAPGFTRE-------TPDGG------PFSPCVPCNCNQHG-TCHPETGVCA-CTDFTTGRHCERCEDGYYGNALTGSPGDCLPCPCPD-----RTTCAQVPETGDVICTNCPTGQRGVRCELCEDGFYGNPLGWGGTVQPCVKCQCN----GNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGY 854 HSP 5 Score: 87.4261 bits (215), Expect = 3.000e-16 Identity = 64/214 (29.91%), Postives = 91/214 (42.52%), Query Frame = 2
Query: 4616 KCNCNG-----YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKND-----KNYDQCEPC---------------------------------QCPSAHYNFAQ-------NCTRIES------SLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYS 5074
KC CNG VCD +TG C C+G+ G+ C+ CR G+Y N +C+PC QC + ++N NC I S + +C CR G G SC+ C+ GF+G +G C++C+C+ GS S+ C+ G C C G +G KCD+C NY + + C CYS
Sbjct: 813 KCQCNGNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGYYGNALANQLNPERKCKPCACNVAGTSGSPNDCHPNTGNCVCLGHVTGRDCGQCEAGYFNLQPGLGCEMCNCNPIGSLSSACHPITGQCMCRTGVEGMSCDTCRMGFFGFSSRG--CRACNCDPMGSTSMQCHNNGTCSCRNGFVGYKCDKCEQNYYQNRLSHQCEECPVCYS 1024 HSP 6 Score: 64.3142 bits (155), Expect = 3.037e-9 Identity = 76/255 (29.80%), Postives = 105/255 (41.18%), Query Frame = 2
Query: 2660 RCKCNNNQESC---DPVTGECRCSYNTMGTECQLCKHGFYGD-----ALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNT-TSGQCF-CLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVT---GCKKC 3370
RCKCN + C + C C +NT G +CQLC FY D A S +C +C+C SGL +C+ E++ +T G+C C EN G CE+C E YF C+ C C+ ++S CD + G C C +V CD C++ + C C+C RGS+ C E G C C NV + C++C G F L + GC KC
Sbjct: 269 RCKCNGHASECVVNEEGRSVCVCEHNTAGVDCQLCA-PFYQDRPWARATAHSANEC----------------VRCNC-------------SGLA---DECV---------FDVEQYRSTGQGGRCLGCRENTAGPHCEQCRENYFRSSALQPCQNCNCNALGSVSLQCDAD-GVCVCWPSVSGVKCDICKSGFHSLGPGGCRLCDCDERGSVG-VCSAEDGRCHCKANVEGQSCNRCKPGSFNLQLENPDGCNKC 479 HSP 7 Score: 57.7658 bits (138), Expect = 2.798e-7 Identity = 46/153 (30.07%), Postives = 75/153 (49.02%), Query Frame = 2
Query: 4616 KCNCNGYSKVCDSVTGDCQN---CMGNREGNKCQLCREGFYKND-------KNYDQCEPCQCPSAHYNFAQNCTRIESSLEL-KC-QCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+C CNG++ C V + ++ C N G CQLC FY++ + ++C C C + + S+ + +C CR +G CE C++ ++ + PC++C+CN GS SL C+ G+C C P V G+KCD C +
Sbjct: 269 RCKCNGHASEC-VVNEEGRSVCVCEHNTAGVDCQLCAP-FYQDRPWARATAHSANECVRCNCSGLADECVFDVEQYRSTGQGGRCLGCRENTAGPHCEQCRENYFRSSALQ-PCQNCNCNALGSVSLQCDADGVCVCWPSVSGVKCDICKSGF 418 HSP 8 Score: 57.3806 bits (137), Expect = 3.916e-7 Identity = 42/148 (28.38%), Postives = 64/148 (43.24%), Query Frame = 2
Query: 4616 KCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAP 5032
+CNC+G + C G C C N G C+ CRE ++++ C+ C C A ++ + C C SG C+ C+ GF+ G C+ CDC+++GS + E G C C V G C++C P
Sbjct: 325 RCNCSGLADECVFDVEQYRSTGQGGRCLGCRENTAGPHCEQCRENYFRSSA-LQPCQNCNCN------ALGSVSLQCDADGVCVCWPSVSGVKCDICKSGFHSLGPGG--CRLCDCDERGSVGVCSAEDGRCHCKANVEGQSCNRCKP 463
BLAST of Laminin subunit alpha-2 vs. Ensembl Zebrafish
Match: lamc3 (laminin, gamma 3 [Source:ZFIN;Acc:ZDB-GENE-060531-121]) HSP 1 Score: 367.081 bits (941), Expect = 3.786e-102 Identity = 294/1010 (29.11%), Postives = 447/1010 (44.26%), Query Frame = 2
Query: 311 CDVCD---NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQN-VNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFG------ITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIK---AKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGY-RRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP----KKKISCKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT------KSCKKCDCDSLGSTSE--NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWN 3205
CD D NRN T + +F +D++ WWQS SM G++Y N VN+T+ L + ++I Y+ LK S RP ++ + + D PW + SC + R + + + L CT +S L G + S + GR S N + + L ++++A I + T + + + + Y+Y++ + +GG+C CN HA +C V E G SV C C+HNT G +C+ C P + RPW C CNC+ ++EC ++ V + GG C+ C+ENT G +CE+C + ++R ++ L PC C C+ LG++ C ++G C+C GV CD+C G+ G C C C GSV C+CK +V C CK G FNL NP+GC C+CFG +NQ + + + + D GWK + +L YL Y +E ++ AP YLGN + SYG EL+ L S+ V ++ + + + + I ++ + + + + EF+ L + L I + Y++Q S L + + D +PH +E C+CP GF G C+ C G+ R + C C C H TC G C CT T G HCE C GYYG+A + + C CPCP T + + +C C GV C+ C +G+YGNP C +C+CN N ++ CD +TG C +C +T G C+ C+ G+YG+AL + CK C C+ G++ +C TG C C + R C C +G + C CNC G +SS C+ +GQC C V G C+ C G+F + + C+ C C P ++ NNG C C + + CDKCE +Y N
Sbjct: 76 CDAFDSELNRNAT--YLTDF-HTDEEPTWWQSQSMFYGVQYPNSVNITLHLGKAFEITYVRLKFYTS-RPESFAIYKRTDEN--GPWQPYQYYSASCRKTYGRDNKGFIRPGDNERTPL------CTDEFSDISPLTGGNVAFSTLEGRPSAYNFDQSLV------LQDWVTATDILISLDRLNTFGDEF-----FKDAKVLRSYFYAISDFSVGGRCKCNGHASEC-VVNEEGRSV--CVCEHNTAGVDCQLCAPFYQDRPWARATAHSANECVRCNCSGLADECVFD---------VEQYRSTGQGGRCLGCRENTAGPHCEQCRENYFR-----SSALQPCQNCNCNALGSVSLQCDADGV---------CVCWPSVSGVKCDICKSGFHSLGPGGCRLCDCDERGSVGVCSAE-DGRCHCKANVEGQS-CNRCKPGSFNLQLENPDGCNKCFCFGHSLACFSSNQYTPIYITSDFLEDPDGWKGVFSRAHEQSLIWKEGEVYLL---PYREENG------FYKAPDKYLGNMLLSYGRTLSMSFTAELEELLPRSVT----VRLEGSGMSVSADLHTQQEPYSQLEKTPINSFSLRLTEKHVNPSVSSFEFLRMLYNLTALHISNAGGQNYTSQLSRVSLESAVQKA---YHPDVLPHALWVEVCSCPAGFAGQFCQLCAPGFTRETPDGGPFSPCVPCNCNQHG-TCHPETGVCA-CTDFTTGRHCERCEDGYYGNALTGSPGDCLPCPCP----DRTTCAQVPETGDVICTNCPTGQRGVRCELCEDGFYGNPLGWGGTVQPCVKCQCNGNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGYYGNALANQLNPERKCKPCACNVAGTSGSPNDCHPNTGNCVCLGHVTGRDCGQCEAGYFNLQPGLGCEMCNCNPIGSLSSA--CHPITGQCMCRTGVEGMSCDTCRMGFFGFSSRGCRACNCDPMGSTSMQCHNNGTCSCRNGFVGYKCDKCEQNYYQN 1010 HSP 2 Score: 123.635 bits (309), Expect = 3.491e-27 Identity = 105/345 (30.43%), Postives = 148/345 (42.90%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY-----GNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP---------NCKKCLCSPHALSA---DCDLNNGKCKCGSNVINRICDKCENLHYWNNRT--DCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC 3505
C +G C C G+ G P C C CN + +C P TG C C+ T G C+ C+ G+YG+ALT S C C C + ++ + C +C T +C+ C G Y + C+ C C ++ C+ +G+C CL + GD CEKC GY+ CK C C+ S DC N G C C +V R C +CE Y+N + C CNC GSL+ C TG C C V C C +G+FG GC+ C P C+N G C C +G C+KC ++ + + C++C
Sbjct: 679 CPAGFAGQFCQLCAPGFTRETPDGGPFSP--CVPCNCNQHG-TCHPETGVCACTDFTTGRHCERCEDGYYGNALTGSPGDCLPCPCPDRTTCAQVPETGDVICTNCPTGQRGVRCELCEDGFYGNPLGWGGTVQPCVKCQCNGNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGYYGNALANQLNPERKCKPCACNVAGTSGSPNDCHPNTGNCVCLGHVTGRDCGQCE-AGYFNLQPGLGCEMCNCNPIGSLSSACHPITGQCMCRTGVEGMSCDTCRMGFFGFSSRGCRACNCDPMGSTSMQCHNNGTCSCRNGFVGYKCDKCEQNYYQNRLSHQCEEC 1019 HSP 3 Score: 116.701 bits (291), Expect = 3.829e-25 Identity = 100/349 (28.65%), Postives = 143/349 (40.97%), Query Frame = 2
Query: 2858 CDCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSADC----DLNNGKCKCGSNVINRICDKCENLHYWN------NRTDCVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTGC----KKCPPCYNGQVCN-------------NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTF 3766
C C F + C C G + C+PCNC G C+ +G C C + G CE+C +GY+ +C C C A D+ C G + C+ CE+ Y N CV C C G+++ CD TG C KC + C KC +GY+G + +KC PC CN N G CVC + G C +C ++ +GC+ CNC GS S+ C+ I+G C C+T G CD C G++GF + C+ C+C G+ S +C G C C+ +G KC C + +
Sbjct: 677 CSCPAGFAGQFCQLCAPGFTRETPDGGPFSPCVPCNCNQHGT------CHPETGVCACTDFTTGRHCERCEDGYYGNALTGSPGDCLPCPCPDRTTCAQVPETGDVICTNCPTGQRGVR--CELCEDGFYGNPLGWGGTVQPCVKCQCN-GNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGYYGNALANQLNPERKCKPC----ACNVAGTSGSPNDCHPNTGNCVCLGHVTGRDCGQCEAGYFNLQPGLGCEMCNCNPIGSLSSACHPITGQCMCRTGVEGMSCDTCRMGFFGFSSRGCRACNCDPMGSTS---MQCHN-NGTCSCRNGFVGYKCDKCEQNYY 1008 HSP 4 Score: 114.775 bits (286), Expect = 1.510e-24 Identity = 143/565 (25.31%), Postives = 219/565 (38.76%), Query Frame = 2
Query: 3419 CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY--GFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRP---IYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKV-----KLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIKG----IPCKSCDCNQQGSQSLNCNEFGIC--------QCSPGVLGIKCDQCAPNY 5038
C NT G C +C E+ + + C+ CNC GS S C+ G+C C + G KCD C G++ G C+ C C E G+ + C G+C CK NV G C C+ G+F GC CFCFG S C + + IY F E G +S H + + L +R Y K + LS + R++ M+ + + + +P V +E GM + H++ + SQ + ++ F + +N +++ F+ L N+T+L + + YT + L ++ + + + VE C C A G CQ G+ +E T G CNCN + C TG C C G C+ C +G+Y N + C PC CP C ++ + ++ C C G G CE C+ GFYGNP+ PC C CN +++ N G+C +C G C++C Y
Sbjct: 354 CRENTAGPHCEQCRENYFRSSALQPCQNCNCNALGSVSLQCDA-DGVCVCWPSVSGVKCDICKSGFHSLGPGGCRLCDCDERGS----VGVCSAEDGRCHCKANVEGQSCNRCKPGSFNLQLENPDGCNKCFCFGHSLACFSSNQY----TPIYITSDFLEDPDGWKGV-FSRAHEQSLIWKEGEVYLLPYREENGFYKAPDKYLGNMLLS-YGRTLSMSFTAELEE-----LLPRSVTVRLEGS----GMSVSA--------------DLHTQQEPY--SQLEKTPINSFSLRLTEKHVNPSVSSFEFLRMLYNLTALHISNAGGQNYTSQ----LSRVSLESAVQKAYHPDVLPHALWVEVCSCPAGFAGQFCQLCAPGFTRE-------TPDGG------PFSPCVPCNCNQHG-TCHPETGVCA-CTDFTTGRHCERCEDGYYGNALTGSPGDCLPCPCPD-----RTTCAQVPETGDVICTNCPTGQRGVRCELCEDGFYGNPLGWGGTVQPCVKCQCN----GNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGY 854 HSP 5 Score: 87.4261 bits (215), Expect = 3.157e-16 Identity = 64/214 (29.91%), Postives = 91/214 (42.52%), Query Frame = 2
Query: 4616 KCNCNG-----YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKND-----KNYDQCEPC---------------------------------QCPSAHYNFAQ-------NCTRIES------SLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYS 5074
KC CNG VCD +TG C C+G+ G+ C+ CR G+Y N +C+PC QC + ++N NC I S + +C CR G G SC+ C+ GF+G +G C++C+C+ GS S+ C+ G C C G +G KCD+C NY + + C CYS
Sbjct: 813 KCQCNGNVDANAVGVCDHMTGRCLKCLGHTTGDHCEKCRLGYYGNALANQLNPERKCKPCACNVAGTSGSPNDCHPNTGNCVCLGHVTGRDCGQCEAGYFNLQPGLGCEMCNCNPIGSLSSACHPITGQCMCRTGVEGMSCDTCRMGFFGFSSRG--CRACNCDPMGSTSMQCHNNGTCSCRNGFVGYKCDKCEQNYYQNRLSHQCEECPVCYS 1024 HSP 6 Score: 64.6994 bits (156), Expect = 2.434e-9 Identity = 76/255 (29.80%), Postives = 105/255 (41.18%), Query Frame = 2
Query: 2660 RCKCNNNQESC---DPVTGECRCSYNTMGTECQLCKHGFYGD-----ALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNT-TSGQCF-CLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVT---GCKKC 3370
RCKCN + C + C C +NT G +CQLC FY D A S +C +C+C SGL +C+ E++ +T G+C C EN G CE+C E YF C+ C C+ ++S CD + G C C +V CD C++ + C C+C RGS+ C E G C C NV + C++C G F L + GC KC
Sbjct: 269 RCKCNGHASECVVNEEGRSVCVCEHNTAGVDCQLCA-PFYQDRPWARATAHSANEC----------------VRCNC-------------SGLA---DECV---------FDVEQYRSTGQGGRCLGCRENTAGPHCEQCRENYFRSSALQPCQNCNCNALGSVSLQCDAD-GVCVCWPSVSGVKCDICKSGFHSLGPGGCRLCDCDERGSVG-VCSAEDGRCHCKANVEGQSCNRCKPGSFNLQLENPDGCNKC 479 HSP 7 Score: 57.7658 bits (138), Expect = 2.907e-7 Identity = 46/153 (30.07%), Postives = 75/153 (49.02%), Query Frame = 2
Query: 4616 KCNCNGYSKVCDSVTGDCQN---CMGNREGNKCQLCREGFYKND-------KNYDQCEPCQCPSAHYNFAQNCTRIESSLEL-KC-QCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+C CNG++ C V + ++ C N G CQLC FY++ + ++C C C + + S+ + +C CR +G CE C++ ++ + PC++C+CN GS SL C+ G+C C P V G+KCD C +
Sbjct: 269 RCKCNGHASEC-VVNEEGRSVCVCEHNTAGVDCQLCAP-FYQDRPWARATAHSANECVRCNCSGLADECVFDVEQYRSTGQGGRCLGCRENTAGPHCEQCRENYFRSSALQ-PCQNCNCNALGSVSLQCDADGVCVCWPSVSGVKCDICKSGF 418 HSP 8 Score: 57.3806 bits (137), Expect = 4.734e-7 Identity = 42/148 (28.38%), Postives = 64/148 (43.24%), Query Frame = 2
Query: 4616 KCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAP 5032
+CNC+G + C G C C N G C+ CRE ++++ C+ C C A ++ + C C SG C+ C+ GF+ G C+ CDC+++GS + E G C C V G C++C P
Sbjct: 325 RCNCSGLADECVFDVEQYRSTGQGGRCLGCRENTAGPHCEQCRENYFRSSA-LQPCQNCNCN------ALGSVSLQCDADGVCVCWPSVSGVKCDICKSGFHSLGPGG--CRLCDCDERGSVGVCSAEDGRCHCKANVEGQSCNRCKP 463
BLAST of Laminin subunit alpha-2 vs. Ensembl Xenopus
Match: LAMA1 (laminin subunit alpha 1 [Source:NCBI gene;Acc:100488009]) HSP 1 Score: 794.653 bits (2051), Expect = 0.000e+0 Identity = 574/1735 (33.08%), Postives = 840/1735 (48.41%), Query Frame = 2
Query: 158 EILSVNVDGLLMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNR--NETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT--HKGY--LEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVA-KCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS--CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQC-SSLSAKNL----KISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDV-IIKCGDFRLFHSVSIEDRRLQRQVF----IYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITLSRGEVH-LALDEMPHIEKCNCPPGFNGLSCE-----SCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS-CKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKS------NELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEH--SKNGKWVISQYQNDILDKF--------IILEDSN------VINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVT-AVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY-KNDKNYDQCEPCQCPSAH-YNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETV 5125
+ L++ + NA I+++ATCG GPE+FC+LV+ + + + NP C +CD N +RHPI A D N WWQSPS+ G Y + +T+DLKQV+Q+ Y+++K +SPRPGNWILERS+DG +MPW ++A + C YN+ R P P+ D+E+ CT+YYS+ L GEI SLINGR S + L++F SARYIR R + TL ADLMT H+ ++ + RYYYS++++ +GG CIC HA C + +S+ +C C+ NTCG +C CCP ++Q PW+P + CE+CNC+ K+ +C Y+Q VA +S+N + GGGVC+NC + T GINCE CI+G+YR V E PC PC+C G++ S V + PG C CK+GY G CD C G++ YPN C+ C CS+ GSV C C CK +V ++ C CK G++NL NPEGC C+CFG++ C +SL + ++SD+ GW ++ G + PK F ++ NS D R + ++W AP YLGN + SYG RY V ++ DS D +I G+ H++S +Q Q F +K + K ++ ++ L ++ L I+A Y + ++ L ++T+ + + +A +E C CPPG+ G SCE S G+ RV L+ G C+ C C H+ CD ++G C +C HNT G HC+ C GYYG+ C+ C CP + C S +C +C SG CD+C GY+GNP C C CN N + CD VTGEC +C NT G+ C++C GFYGDA+ K+C C C S S C TG C+C+ N + ++CD+C++ L I C + S F S + FC + +C V +P + +G+ +++ + HY C PCNC + GS++ C+ + G C C VA C C G++ GC C + CN G C+CPP+T+G C+ C + W + +GC CNC I GS + C+ SG C CK F G C+ C G+ +P+C C C +GT+S + C++ G C CK NV+G+ C C+ GTF GC CFC GVS+ C + + S+ SL S +D +T + + +P M + V + NS + + +S L NK M SY G V + ++ I DF H K G++ DI I L ++N V + +++R FM L NV + +K Y ++ I + V + G+ V +E C C G CQ+ GYYKE P T S+R ++ C CN +S++CD TG CQ+C N +G+ C +C GFY + C C CP + +F+ C +E + +C C GY G+ CE C G+YGNP + G C+ CDCN GS + +C+ G C C PGV G CD C +++ + C SC CT LL +D+L+ +
Sbjct: 51 KTLALTPFSYALFNAHISTNATCGEMGPEMFCKLVEHVPGRPVRNPQ----CRICDQNSINPKERHPISNAI-DGTNDWWQSPSIQNGRVYHWITITLDLKQVFQVAYVIIKAANSPRPGNWILERSVDGVEFMPWQYYAISDTECLTRYNVTPRIGP---------PTYKRDNEVICTSYYSRLVPLEHGEIHTSLINGRPSADDPS--------PALLDFTSARYIRLRLQRIRTLNADLMTLSHRDLRDVDPIVTRRYYYSVKDLSVGGMCICYGHARSCPW---DEVSLKFQCQCERNTCGESCNECCPGYHQNPWRPGTISVGNKCEKCNCHNKAEDCYYDQGVADRNMSLNINNQYIGGGVCLNCTQFTAGINCETCIEGYYRQHKVSPYEDNPCIPCECDVFGSVNSVCVVDDNHAKPGSSPGQCQCKEGYTGTKCDQCKFGFKGYPN--CVRCNCSLVGSVNED--PCAEQCVCKDNVEGEN-CDRCKAGFYNLQERNPEGCTECFCFGVSGVCDASLYTVYILLMVQVSDVVGWHISDLQGTQRIQPKQDQFDGPHQISVNNS--DARKAFQSAFFWDAPSSYLGNKLVSYGGYLRYTVSYDIPE-------DSADSELISDGN---GHTLSTRGDGVQLQPFEEHTAELKLVPDNFVDFSTRKPVDRDRLMTVLANINHLKIRANYNLAKNTVTRLSSVTMDIAQPNVIAFLPAVQVENCECPPGYAGTSCEITSLPSNLPGFYRVGGTLFGGICQPCECNGHATECD-IHGVCFDCQHNTTGPHCDQCLPGYYGNPLYGTPQDCQPCTCPLSIPSNNFSPTCHLSSDGEVICDQCPPGYSGSQCDKCANGYFGNPTMPGGLCVPCDCNGNVDPSETGHCDSVTGECLKCIDNTAGSHCEICADGFYGDAVFAKNCTACRCHPNSSISATCHHETGLCECKINVLGQRCDTCSNFLMYIYIHYI---CRL---YVSLLFFKLGSMRAFCCV-ITCTKCTYLVGHLPGWPTSHSVHMGKVGGGYPINSFSGRSAAFRELLS-------HGHYGLTTGLGCHPCNCSQMGSISDACN-DLGQCPCVPGVAGLKCDICAHGFYAFQEGGCSPCDCAHTQNNCNPMTGECICPPHTLGPKCDSCEANYWGHDPELGCLNCNCSIAGSQTLQCDLSSGQCPCKDGFGGHNCNTCAFGFRQYPSCIACDCDPSGTQSAWCDDTQTVCSCEEDAGICACKGNVVGSYCNECKTGTFALRADNPLGCTPCFCSGVSQLCTETDGYVRIPISLIPDQPLLHVVSL-SNQDGTTDGVFSEFPE-------MLLDAGM-VQQRLNS-------EAFYWQLSDQFLGNK------------MMSYGGWLTY---VITYYAVGDSGISDFEPHILMKGGRFSKLVIYADIPPPANRLGSEIQISLTENNWKYFNAVSDRSVSRSDFMSVLENVDYILIKASYGYGLHQSRIANISMDIAVEASEMHSGREVAHLIEICECPLGYTGLSCQDCAPGYYKEK---PMET---SVRGSQSLIARCVPCQCNNHSEICDPDTGKCQDCRDNTDGHHCHVCASGFYGRVTGTIADCSLCACPKGNTQSFSPTCV-LEGISDFRCDACLPGYEGQYCERCSLGYYGNPNEPGGTCQKCDCNFSGSLNNDCDGMTGQCSCKPGVTGRLCDTCEIRHILLDQECISCDDDCTGPLLDDLDSLHMAM 1689
BLAST of Laminin subunit alpha-2 vs. Ensembl Xenopus
Match: wdcp (WD repeat and coiled coil containing [Source:Xenbase;Acc:XB-GENE-5729803]) HSP 1 Score: 732.635 bits (1890), Expect = 0.000e+0 Identity = 456/1179 (38.68%), Postives = 622/1179 (52.76%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNR--NETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIY--------NRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQK--PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNN-PTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDS---PDVIIKCGDFRLFHSVSIEDRRLQRQVF------IYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITLS-RGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP-RLDY-QMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQES-----CDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSC---TSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYN---------GQVCNNE----------GICVCPPNTIGNLCNKCSEDSWDFN--ETIGCKKCNCY 3514
L NA+IT++ATCG G E FC+LV+ + Q P + C CD + N RHPI A + N WWQSPS+ G++Y V VT+DL+QV+QI YI++K +SPRPGNWILERSLDG + PW ++A T C ++ + A+ S DDE+ CT++YS+ L +GEI SLI GR S + P+ L+ F SARYIR R + TL ADLM L QS Y RY+YS+++I +GG CIC HA C + C C+HNTCG +C+ CCP FNQ+PWK CE CNC+ KS EC Y+Q VA +LS++ +G GGGVCINC NT GINCE C+ FYRP V T+ YPC PC C +G++ E + PG C CK G+ G CD C++GY YP EC+ C CS+ GS T+ C PC CK +V + C CK GYFNL NP+GC+ C+C G+++ C+ KI+D+ GW +T +G P F L+ N D R P VYYW+APP YL + +T+ G Y + + +++K +DS P++II+ + +S + +Q F I + P ++I + ++ EF+ L V+KLLI+A Y + Q++I L ++TL G+ + +E C CP GF+G SCESC G RRVN L+ G CE C CY H++ C + GEC +C HNT G +C+ C G+YG+ CK C CP +D + C + VC C +G C+RC EGYYG P + SCK CKCNNN + CD TG C C G C C G++GDAL + C+ C+C GS S +C+ TG+C CR N + RQCD C T GL + C+PCNC G S+ F SGQC C V G +C+ C G++++ C C C PH + +CD + G C C N I CDKC H+ ++ T C CNC GSL+ +C+ +TG C C N + CS C +GY +K P C N Q C+ E G C C N G LC++C ++ + +GC C C+
Sbjct: 23 LATNARITTNATCGEKGQETFCKLVEHVPGQ----PLRYSQCRTCDQKSSNPNLRHPISNA-INGKNSWWQSPSIQNGMQYHYVTVTLDLQQVFQIAYIIIKAANSPRPGNWILERSLDGVNFKPWQYYAITESECLTRYKVK------PQPAQASFAKDDEVICTSHYSKIHPLENGEIFTSLIIGRPSAEDPS-------PE-LLEFTSARYIRLRLQRIRTLNADLM----MLSQSTYKDIDPIVTRRYFYSIKDISVGGMCICYGHARSCP--FNLATKKSSCECEHNTCGDSCDRCCPGFNQKPWKAGTFLLKHECEPCNCHWKSEECYYDQQVADKRLSLDILGNYSGGGVCINCTSNTAGINCETCVDRFYRPKGVSPTQPYPCQPCSCDPIGSLHDICVKDEKQAPRGMLPGSCHCKSGFAGERCDKCAVGYIGYP--ECVRCNCSLIGS--TNKDPCVDPCICKDNVEGQN-CDQCKLGYFNLQKDNPQGCDQCFCSGVSSICTGSRLAYTKITDMNGWYVTDLSGYTRVAPAKDGFDGPHQLSITNR--DARAQGLPEVYYWSAPPTYLRSKVTAVGGHLSYTISYD--ASMKTDSVDSTNYPNIIIEGNGIK----ISSQQETIQLGPFEEITETIVLHPDSFRI--YGTEFQVSRREFMMVLANVNKLLIRATYSNGQEAIYRLSSVTLEVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPGNRRVNGTLFGGSCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQ-SAQGCVPCNCNSFG---SKSFDCDDSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPCEC-PH-VGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGY--------RKYPTCVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCF 1147 HSP 2 Score: 242.276 bits (617), Expect = 6.737e-63 Identity = 163/458 (35.59%), Postives = 222/458 (48.47%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEG-------YYGNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYG---DALTKSCKKCDCDSLGSTSEN------CDKLTG-KCD-CRTNFVNRQCDSCTSGLYLKDKK----CIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGT------KSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
+C SG C+ C+ G +G SC+ C+C + + C VTGEC C +NT G C C GFYG + + CK C C L S N DK G CD CR + ++C+ C G Y + + C PC C +S+ C++ +G C C + G C KC EGYF C+ C C + ++S C+ G+C+C +NVI R CD+C + + CVPCNC GS + CD ++G C+C V + C CD G++ GC C + G C+ + G CVCPPNTIG C+KC+ + W ++ GCK CNC GS S CN +G C C+ F G KC C GY +P C C C G+ + E C + TGQC CK NV G C C+ TF GC SC+CFG++ C
Sbjct: 703 QCPSGFSGTSCESCLPGNRRVNGTLFGG-----SCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSC-PLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQSAQGCVPCNCNSFGSKSFDCD-DSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPTCVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCFGLATHC 1153 HSP 3 Score: 231.876 bits (590), Expect = 9.171e-60 Identity = 199/700 (28.43%), Postives = 286/700 (40.86%), Query Frame = 2
Query: 1007 VAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNR---KSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGT-----IGSCVSNGEDIGDQ-----------------------KPGD-----------CICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSV---------RTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSS--------LSAKN----LKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCG---DFRLF--HSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRL--DYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCN---NNQESCDPVTGECRCSYNTMGTECQLC--KHGFYGDALTKSCKKCDCDSLGSTSENCDKL 2848
+C C++N G C+ C P + C CNCN KS +C + G C CQ G C+ C GFY + CTPC+C +G G CV IG+Q PG C C+ + G C C +GY +YP C+ C C + GS + S CNCK++V LC CK F L NP GC CYCFG+ C+ L+ K L + DL TT G P+ + E+ + + +YW P + G +T+YG +Y + E + S + P VII+ G + R+ + + ++ +L R I + +WK + ++E+F+ L + +LIKA Y S + + I+L E + P IEKC CP G++GLSCE+C G+ RV G C C C HS+ CD + CQ C HNTAG HCE CA G+YG + + + CK C CP L + C+ + +Y C C G +C+RC GY GNP SC+ C+CN + C P TG+C C + G +C C +H G C CD + G ++ ++L
Sbjct: 912 TGQCQCRNNVIGRQCDECLP--QTFGLQSAQGCVPCNCNSFGSKSFDC-------------------DDSGQC-RCQPGVTGKKCDHCDHGFYD------FQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPT--CVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGV-LCDRCKPSTFGLHVGNPLGCSSCYCFGLATHCTEADGLIRMWLTLKPDQTVLPLVDLTQQHRTT-KGVTYQHPEIVAMIDQVKEDLHAEP-------FYWRLPQQFEGRKLTAYGGKLKYAIYFEARDETGPSTYE-PQVIIRGGPHANHRIIVRNMAAPQNGQLTRHD-IEMTEHDWKYLGSVDGRPVSREDFMDVLYDIQYILIKAAYGSVLRQTRISEISLEVAEEGTSFTISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGRRTGPTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRG-SPDDCKPCACPLLISSNNFSPTCTLQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLSMICHPTTGQCTCKTGSTGLKCAGCTDRHAREGPV----CVFCDDECTGLLLDDLEQL 1565 HSP 4 Score: 176.407 bits (446), Expect = 6.787e-43 Identity = 193/723 (26.69%), Postives = 283/723 (39.14%), Query Frame = 2
Query: 3086 CLCSPHALSADCDLNNGK--CKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICV-CPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI---------LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNA---NYPRRFGW--PLTMFRPIYVNV---MKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSN---LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSA--HYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEFGICQ--------CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
C+C HA S +L K C+C N CD+C N W T +C PCNC ++ C Q VA + S LD+ G Y+G G+C+ C NT G C C + + + C+ C+C GS +IC K + G C CK+ F G +CD+C GY G+P C C+C+ G+ T D C CK NV G C C+ G F GC CFC GVS C +N V + + K+ + S + +A P + W P T R V + S+D S+ S+ + + G +I Q E G F +I + ++ DS I ++R FMM L NV L ++ + ++ +T +V + GK +VE C C + G C++ + G N +G++ G+ + C C G++ C VTG+C +C N G C C GFY N + + C+PC CP NF+ C ++ + L C CR GY+G+ CE C +G+YG P + G CK C CN +L+ + G C C PG+ G C++CA Y +E + CQSC
Sbjct: 264 CICYGHARSCPFNLATKKSSCECEHNTCGDSCDRCCPGFNQKPWKAGTFLLKHECEPCNC---------HWKSEECYYDQQVADKRLS--------LDILGN------YSGG-----GVCINCTSNTAGINCETCVDRFYRPKGVSPTQPYPCQPCSCDPIGSLHDICVKDEKQAPRGMLPGSCHCKSGFAGERCDKCAVGYIGYPECVRCNCSLIGS-----TNKDPCVDPCICKDNVEGQNCDQCKLGYFNLQKDNPQGCDQCFCSGVSSICTGSRLAYTKITDMNGWYVTDLSGYTRVAPAKDGFDGPHQLSITNRDARAQGLPEVYYWSAPPTYLRSKVTAVGGHLSYTISYDASMKTDSVDSTNYPNIIIEGNGIKISSQQETIQL---GPF-----------------------------------EEITETIVLHPDSFRIYGTEFQVSRREFMMVLANVNKLLIRATYSNGQEAIYRLSSVTLEVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPG---------NRRVNGTLFGGS-----CEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTC-HLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCN----NNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSC 896 HSP 5 Score: 150.599 bits (379), Expect = 4.244e-35 Identity = 173/664 (26.05%), Postives = 286/664 (43.07%), Query Frame = 2
Query: 4313 LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVME--TTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIK-NIYKEMPNITLAAS---VLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKT-IMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNL-IKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDS---LKKLLAE-----FNSNKS-EIVENIKNIDKEHEKLLKQYK 6232
++R+ FM L ++ + +K V +I I+ +V E T+ I R +EKC C G C+ G+Y+ P RTG L C CNG+S +CD + CQ C N G+ C+ C GFY + D C+PC CP + NF+ CT ++ + + +C C +GY G+ CE C G+ GNP + G C+ C+CN GS S+ C+ G C C G G+KC C + E C C CT LL ++ LN+ V++ LP N + ++ LL+ +L Q +++ E+++L R KV D T D T +QL + +K + +K N +PN TL S + KD+ + EL + Q + D++ + L N+ + E + N++ N + K + L E++ D+ + R+ R KI+ D +S ++K++ + V++ + + K V T ++ Q +N++ N+ + LT EQ E +N+T++I D + E N++ IDS L +LAE FN+ + NIK++ + E K+ K
Sbjct: 1300 VSREDFMDVLYDIQYILIKAAYGSV-LRQTRISEISLEVAEEGTSFTISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGR------RTG-PTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPCACPLLISSNNFSPTCT-LQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLSMICHPTTGQCTCKTGSTGLKCAGCTDRHAREGPVCVFCDDECTGLLLDDLEQLNQMVMSVNLSGPLPPPYKTLFTFGNQTQEL-KHLLSPQRAPERL----------LQLAQNNQDTLVIEMDELLNRAAKVTADGEQTGKDAERTNERAKQL---EDFVKETLRASKAVKLNETLGIPNKTLDKSFQELQKDIDSMMAELRRKSLRNQKNTADDELKAAEALLNRVKKLFLEAHKKNEDLNNEVKEK-------LGLFEEKLDDVWDLLREA-------RGKIKAADDLSTV--NQKNMTELEVKKKQGVEKGKKETESVLGDGDSIMTEVNNLAQIINEAENVKLPPLT---------EQLNEKINNLTQEIARRDLPEQILQAE---NQAAQLIDSSAVLDGILAEAKNLSFNATVAFNAYSNIKDLIDDAEDTAKEAK 1912 HSP 6 Score: 125.561 bits (314), Expect = 1.505e-27 Identity = 107/337 (31.75%), Postives = 139/337 (41.25%), Query Frame = 2
Query: 2951 EIQGRISSEKFCNTTSGQCFCLENVNGDRCEKC------VEGYFNYPNCKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWN----NRTDCVPCNC----GRGSLNPKCDMETGNC----KCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-------------EGIC-VCPPNTIGNLCNKCSEDSW-DFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQ---PCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNH 3850
E+ G SS + C C +G CE C V G +C+ C C H + C G+C C N R CD C Y N DC PC+C + +P C ++ C + C +C GY+G C PC CNN G C VC P G CNKC+E + D E GC+ CNC++NGS S CN +G C C+ G +CD C +G + Q PC+C G+KS + CD +GQC+C+ V G KC C G + EGGC C C V C A H
Sbjct: 684 EVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPGNRRVNGTLFGGSCEPCRCYGH--TDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCK----CNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQSAQGCVPCNCNSFGSKSFD---CDD-SGQCRCQPGVTGKKCDHCDHGFYDFQ---EGGCTPCECPHVGNNCDADTGH 1007 HSP 7 Score: 75.0998 bits (183), Expect = 2.939e-12 Identity = 60/197 (30.46%), Postives = 85/197 (43.15%), Query Frame = 2
Query: 2543 SYSSENYVCHKC--LHNTSGVHCDRCIEGYY----GNPKKKI-----SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGST---SENCDKLTGKCD-----CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVE 3055
+ S Y+ KC H SG+ C+ C G+Y P ++ +C C+CN + + CDP + C+ C +NT G C+ C GFYG CK C C L S+ S C L G D C + + C+ C SG + C C C G +S C+ T+GQC C G +C C +
Sbjct: 1345 TISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGRRTGPTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPCACPLLISSNNFSPTC-TLQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLS--MICHPTTGQCTCKTGSTGLKCAGCTD 1538 HSP 8 Score: 72.7886 bits (177), Expect = 1.387e-11 Identity = 54/160 (33.75%), Postives = 75/160 (46.88%), Query Frame = 2
Query: 4613 KKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCT-RIESSLELKCQCRIGYSGKSCENC-QQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPN-YVVEKKTCQSC 5068
K C CN + CDS TG C C G C C EG++ + C+ C C H N + + + E+ +CQCR G+ C+ C Q F +G C C+CN GS+S +C++ G C+C PGV G KCD C Y ++ C C
Sbjct: 841 KPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNC---HVNGSVSTSCNTETG---QCQCRNNVIGRQCDECLPQTFGLQSAQG--CVPCNCNSFGSKSFDCDDSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPC 992 HSP 9 Score: 63.5438 bits (153), Expect = 7.982e-9 Identity = 49/142 (34.51%), Postives = 71/142 (50.00%), Query Frame = 2
Query: 4619 CNCNG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
C+ NG S C++ TG CQ C N G +C C + ++ C PC C S F + S + C+C+ G +GK C++C GFY G C C+C G+ NC+ + G C C P +G +CD+CAPN+
Sbjct: 898 CHVNGSVSTSCNTETGQCQ-CRNNVIGRQCDECLPQTF-GLQSAQGCVPCNCNS----FGSKSFDCDDSGQ--CRCQPGVTGKKCDHCDHGFYDFQEGG--CTPCECPHVGN---NCDADTGHCVCPPNTIGEQCDKCAPNH 1026 HSP 10 Score: 57.3806 bits (137), Expect = 6.141e-7 Identity = 54/185 (29.19%), Postives = 70/185 (37.84%), Query Frame = 2
Query: 3218 CVPCNC-GRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
CVPC C G + CD ++ C+ C N A C +C G++G+ C PC CP N +F+ T C + G+ C AC + G C+RC GY G P +CQ C C G+ S C TGQC CK G KC C
Sbjct: 1393 CVPCQCNGHSDI---CDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPC------------ACPLLISSN----------NFSPT-------CTLQGTNDYRCT------ACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLS---MICHPTTGQCTCKTGSTGLKCAGC 1536 HSP 11 Score: 56.225 bits (134), Expect = 1.531e-6 Identity = 64/227 (28.19%), Postives = 88/227 (38.77%), Query Frame = 2
Query: 4619 CNCNGY---SKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSA----------------------------HYNF-------AQNCTRIESSLELKCQ-------CRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE----------FGICQCSPGVLGIKCDQCAPN----YVVEKKTCQSCY-----SGCTK-DLLVRI 5104
CNCN + S CD +G C+ C G KC C GFY D C PC+CP H+ + A NC+ SL ++C CR +SG C +C+ G+ P C +C C GSQ C++ G C C V G+ CD+C P+ +V C SCY + CT+ D L+R+
Sbjct: 945 CNCNSFGSKSFDCDD-SGQCR-CQPGVTGKKCDHCDHGFY--DFQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSN-PGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPT----CVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCFGLATHCTEADGLIRM 1162
BLAST of Laminin subunit alpha-2 vs. Ensembl Xenopus
Match: wdcp (WD repeat and coiled coil containing [Source:Xenbase;Acc:XB-GENE-5729803]) HSP 1 Score: 732.25 bits (1889), Expect = 0.000e+0 Identity = 456/1179 (38.68%), Postives = 622/1179 (52.76%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNR--NETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIY--------NRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQK--PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNN-PTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDS---PDVIIKCGDFRLFHSVSIEDRRLQRQVF------IYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITLS-RGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP-RLDY-QMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQES-----CDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSC---TSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYN---------GQVCNNE----------GICVCPPNTIGNLCNKCSEDSWDFN--ETIGCKKCNCY 3514
L NA+IT++ATCG G E FC+LV+ + Q P + C CD + N RHPI A + N WWQSPS+ G++Y V VT+DL+QV+QI YI++K +SPRPGNWILERSLDG + PW ++A T C ++ + A+ S DDE+ CT++YS+ L +GEI SLI GR S + P+ L+ F SARYIR R + TL ADLM L QS Y RY+YS+++I +GG CIC HA C + C C+HNTCG +C+ CCP FNQ+PWK CE CNC+ KS EC Y+Q VA +LS++ +G GGGVCINC NT GINCE C+ FYRP V T+ YPC PC C +G++ E + PG C CK G+ G CD C++GY YP EC+ C CS+ GS T+ C PC CK +V + C CK GYFNL NP+GC+ C+C G+++ C+ KI+D+ GW +T +G P F L+ N D R P VYYW+APP YL + +T+ G Y + + +++K +DS P++II+ + +S + +Q F I + P ++I + ++ EF+ L V+KLLI+A Y + Q++I L ++TL G+ + +E C CP GF+G SCESC G RRVN L+ G CE C CY H++ C + GEC +C HNT G +C+ C G+YG+ CK C CP +D + C + VC C +G C+RC EGYYG P + SCK CKCNNN + CD TG C C G C C G++GDAL + C+ C+C GS S +C+ TG+C CR N + RQCD C T GL + C+PCNC G S+ F SGQC C V G +C+ C G++++ C C C PH + +CD + G C C N I CDKC H+ ++ T C CNC GSL+ +C+ +TG C C N + CS C +GY +K P C N Q C+ E G C C N G LC++C ++ + +GC C C+
Sbjct: 19 LATNARITTNATCGEKGQETFCKLVEHVPGQ----PLRYSQCRTCDQKSSNPNLRHPISNA-INGKNSWWQSPSIQNGMQYHYVTVTLDLQQVFQIAYIIIKAANSPRPGNWILERSLDGVNFKPWQYYAITESECLTRYKVK------PQPAQASFAKDDEVICTSHYSKIHPLENGEIFTSLIIGRPSAEDPS-------PE-LLEFTSARYIRLRLQRIRTLNADLM----MLSQSTYKDIDPIVTRRYFYSIKDISVGGMCICYGHARSCP--FNLATKKSSCECEHNTCGDSCDRCCPGFNQKPWKAGTFLLKHECEPCNCHWKSEECYYDQQVADKRLSLDILGNYSGGGVCINCTSNTAGINCETCVDRFYRPKGVSPTQPYPCQPCSCDPIGSLHDICVKDEKQAPRGMLPGSCHCKSGFAGERCDKCAVGYIGYP--ECVRCNCSLIGS--TNKDPCVDPCICKDNVEGQN-CDQCKLGYFNLQKDNPQGCDQCFCSGVSSICTGSRLAYTKITDMNGWYVTDLSGYTRVAPAKDGFDGPHQLSITNR--DARAQGLPEVYYWSAPPTYLRSKVTAVGGHLSYTISYD--ASMKTDSVDSTNYPNIIIEGNGIK----ISSQQETIQLGPFEEITETIVLHPDSFRI--YGTEFQVSRREFMMVLANVNKLLIRATYSNGQEAIYRLSSVTLEVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPGNRRVNGTLFGGSCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQ-SAQGCVPCNCNSFG---SKSFDCDDSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPCEC-PH-VGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGY--------RKYPTCVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCF 1143 HSP 2 Score: 242.276 bits (617), Expect = 7.636e-63 Identity = 163/458 (35.59%), Postives = 222/458 (48.47%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEG-------YYGNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYG---DALTKSCKKCDCDSLGSTSEN------CDKLTG-KCD-CRTNFVNRQCDSCTSGLYLKDKK----CIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGT------KSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
+C SG C+ C+ G +G SC+ C+C + + C VTGEC C +NT G C C GFYG + + CK C C L S N DK G CD CR + ++C+ C G Y + + C PC C +S+ C++ +G C C + G C KC EGYF C+ C C + ++S C+ G+C+C +NVI R CD+C + + CVPCNC GS + CD ++G C+C V + C CD G++ GC C + G C+ + G CVCPPNTIG C+KC+ + W ++ GCK CNC GS S CN +G C C+ F G KC C GY +P C C C G+ + E C + TGQC CK NV G C C+ TF GC SC+CFG++ C
Sbjct: 699 QCPSGFSGTSCESCLPGNRRVNGTLFGG-----SCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSC-PLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQSAQGCVPCNCNSFGSKSFDCD-DSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPTCVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCFGLATHC 1149 HSP 3 Score: 231.876 bits (590), Expect = 8.936e-60 Identity = 199/700 (28.43%), Postives = 286/700 (40.86%), Query Frame = 2
Query: 1007 VAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNR---KSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGT-----IGSCVSNGEDIGDQ-----------------------KPGD-----------CICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSV---------RTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSS--------LSAKN----LKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCG---DFRLF--HSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRL--DYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCN---NNQESCDPVTGECRCSYNTMGTECQLC--KHGFYGDALTKSCKKCDCDSLGSTSENCDKL 2848
+C C++N G C+ C P + C CNCN KS +C + G C CQ G C+ C GFY + CTPC+C +G G CV IG+Q PG C C+ + G C C +GY +YP C+ C C + GS + S CNCK++V LC CK F L NP GC CYCFG+ C+ L+ K L + DL TT G P+ + E+ + + +YW P + G +T+YG +Y + E + S + P VII+ G + R+ + + ++ +L R I + +WK + ++E+F+ L + +LIKA Y S + + I+L E + P IEKC CP G++GLSCE+C G+ RV G C C C HS+ CD + CQ C HNTAG HCE CA G+YG + + + CK C CP L + C+ + +Y C C G +C+RC GY GNP SC+ C+CN + C P TG+C C + G +C C +H G C CD + G ++ ++L
Sbjct: 908 TGQCQCRNNVIGRQCDECLP--QTFGLQSAQGCVPCNCNSFGSKSFDC-------------------DDSGQC-RCQPGVTGKKCDHCDHGFYD------FQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPT--CVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGV-LCDRCKPSTFGLHVGNPLGCSSCYCFGLATHCTEADGLIRMWLTLKPDQTVLPLVDLTQQHRTT-KGVTYQHPEIVAMIDQVKEDLHAEP-------FYWRLPQQFEGRKLTAYGGKLKYAIYFEARDETGPSTYE-PQVIIRGGPHANHRIIVRNMAAPQNGQLTRHD-IEMTEHDWKYLGSVDGRPVSREDFMDVLYDIQYILIKAAYGSVLRQTRISEISLEVAEEGTSFTISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGRRTGPTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRG-SPDDCKPCACPLLISSNNFSPTCTLQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLSMICHPTTGQCTCKTGSTGLKCAGCTDRHAREGPV----CVFCDDECTGLLLDDLEQL 1561 HSP 4 Score: 176.022 bits (445), Expect = 8.368e-43 Identity = 193/723 (26.69%), Postives = 283/723 (39.14%), Query Frame = 2
Query: 3086 CLCSPHALSADCDLNNGK--CKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICV-CPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI---------LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNA---NYPRRFGW--PLTMFRPIYVNV---MKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSN---LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSA--HYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEFGICQ--------CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
C+C HA S +L K C+C N CD+C N W T +C PCNC ++ C Q VA + S LD+ G Y+G G+C+ C NT G C C + + + C+ C+C GS +IC K + G C CK+ F G +CD+C GY G+P C C+C+ G+ T D C CK NV G C C+ G F GC CFC GVS C +N V + + K+ + S + +A P + W P T R V + S+D S+ S+ + + G +I Q E G F +I + ++ DS I ++R FMM L NV L ++ + ++ +T +V + GK +VE C C + G C++ + G N +G++ G+ + C C G++ C VTG+C +C N G C C GFY N + + C+PC CP NF+ C ++ + L C CR GY+G+ CE C +G+YG P + G CK C CN +L+ + G C C PG+ G C++CA Y +E + CQSC
Sbjct: 260 CICYGHARSCPFNLATKKSSCECEHNTCGDSCDRCCPGFNQKPWKAGTFLLKHECEPCNC---------HWKSEECYYDQQVADKRLS--------LDILGN------YSGG-----GVCINCTSNTAGINCETCVDRFYRPKGVSPTQPYPCQPCSCDPIGSLHDICVKDEKQAPRGMLPGSCHCKSGFAGERCDKCAVGYIGYPECVRCNCSLIGS-----TNKDPCVDPCICKDNVEGQNCDQCKLGYFNLQKDNPQGCDQCFCSGVSSICTGSRLAYTKITDMNGWYVTDLSGYTRVAPAKDGFDGPHQLSITNRDARAQGLPEVYYWSAPPTYLRSKVTAVGGHLSYTISYDASMKTDSVDSTNYPNIIIEGNGIKISSQQETIQL---GPF-----------------------------------EEITETIVLHPDSFRIYGTEFQVSRREFMMVLANVNKLLIRATYSNGQEAIYRLSSVTLEVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPG---------NRRVNGTLFGGS-----CEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTC-HLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCN----NNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSC 892 HSP 5 Score: 149.058 bits (375), Expect = 1.123e-34 Identity = 160/618 (25.89%), Postives = 279/618 (45.15%), Query Frame = 2
Query: 4313 LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVME--TTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCPS--AHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINF--------KWKTLPSTLDLRLNKIN--ISIQMSSELLNSI--NKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLET-IKNIYKEMPNITL--AASVLKDVLKYVDELNQIIK--YTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLI--SINVREIEDLMKNLSS--NHLVSKSLIIPDTLKKHVEQQLNKSSNLIKK-------LTKDFNFLHNAEQAKEAYSNITRDIL 6058
++R+ FM L ++ + +K V +I I+ +V E T+ I R +EKC C G C+ G+Y+ P RTG L C CNG+S +CD + CQ C N G+ C+ C GFY + D C+PC CP + NF+ CT ++ + + +C C +GY G+ CE C G+ GNP + G C+ C+CN GS S+ C+ G C C G G+KC C + E C C CT LL ++ LN+ V++ +KTL T + ++ +S Q + E L + N L E+ + K+ D ++ + E+ R ++ + ++ T ++ + +E LGI N+ + + L+ I ++ E+ +L + D LK + L +K + ++H +++ +NN +K K + +E + + L K K + + + M +L++ + + K + + D+I + +I + NV+ + L + L+ N+L + I L + + Q N+++ LI L + N NA A AYSNI +D++
Sbjct: 1296 VSREDFMDVLYDIQYILIKAAYGSV-LRQTRISEISLEVAEEGTSFTISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGR------RTG-PTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPCACPLLISSNNFSPTCT-LQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLSMICHPTTGQCTCKTGSTGLKCAGCTDRHAREGPVCVFCDDECTGLLLDDLEQLNQMVMSVNLSGPLPPPYKTL-FTFGNQTQELKHLLSPQRAPERLLQLAQNNQDTLVIEMDELLNRAAKVTADGEQTGKDAERTNERAKQLEDFVKETLHIYDKAVKLNETLGIPNKTLDKSFQELQKDIDSMMAELRRKSLRNQKNTADDELKAAEALLNRVKKLFLEAHKKNEDLNNE--VKEKLGLFEEKLDDVWDLLREARGKIKAADDLSTVNQKNMTELERKKQGVEKGKKETESVLGDGDSIMTEVNNLAQIINEAENVK-LPPLTEQLNEKINNLTQE--IARRDLPEQILQAENQAAQLIDSSAVLDGILAEAKNLSFNATVAFNAYSNI-KDLI 1897 HSP 6 Score: 125.561 bits (314), Expect = 1.517e-27 Identity = 107/337 (31.75%), Postives = 139/337 (41.25%), Query Frame = 2
Query: 2951 EIQGRISSEKFCNTTSGQCFCLENVNGDRCEKC------VEGYFNYPNCKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWN----NRTDCVPCNC----GRGSLNPKCDMETGNC----KCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-------------EGIC-VCPPNTIGNLCNKCSEDSW-DFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQ---PCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNH 3850
E+ G SS + C C +G CE C V G +C+ C C H + C G+C C N R CD C Y N DC PC+C + +P C ++ C + C +C GY+G C PC CNN G C VC P G CNKC+E + D E GC+ CNC++NGS S CN +G C C+ G +CD C +G + Q PC+C G+KS + CD +GQC+C+ V G KC C G + EGGC C C V C A H
Sbjct: 680 EVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPGNRRVNGTLFGGSCEPCRCYGH--TDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCK----CNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQSAQGCVPCNCNSFGSKSFD---CDD-SGQCRCQPGVTGKKCDHCDHGFYDFQ---EGGCTPCECPHVGNNCDADTGH 1003 HSP 7 Score: 75.0998 bits (183), Expect = 3.091e-12 Identity = 60/197 (30.46%), Postives = 85/197 (43.15%), Query Frame = 2
Query: 2543 SYSSENYVCHKC--LHNTSGVHCDRCIEGYY----GNPKKKI-----SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGST---SENCDKLTGKCD-----CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVE 3055
+ S Y+ KC H SG+ C+ C G+Y P ++ +C C+CN + + CDP + C+ C +NT G C+ C GFYG CK C C L S+ S C L G D C + + C+ C SG + C C C G +S C+ T+GQC C G +C C +
Sbjct: 1341 TISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGRRTGPTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPCACPLLISSNNFSPTC-TLQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLS--MICHPTTGQCTCKTGSTGLKCAGCTD 1534 HSP 8 Score: 72.7886 bits (177), Expect = 1.410e-11 Identity = 54/160 (33.75%), Postives = 75/160 (46.88%), Query Frame = 2
Query: 4613 KKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCT-RIESSLELKCQCRIGYSGKSCENC-QQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPN-YVVEKKTCQSC 5068
K C CN + CDS TG C C G C C EG++ + C+ C C H N + + + E+ +CQCR G+ C+ C Q F +G C C+CN GS+S +C++ G C+C PGV G KCD C Y ++ C C
Sbjct: 837 KPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNC---HVNGSVSTSCNTETG---QCQCRNNVIGRQCDECLPQTFGLQSAQG--CVPCNCNSFGSKSFDCDDSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPC 988 HSP 9 Score: 63.5438 bits (153), Expect = 8.253e-9 Identity = 49/142 (34.51%), Postives = 71/142 (50.00%), Query Frame = 2
Query: 4619 CNCNG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
C+ NG S C++ TG CQ C N G +C C + ++ C PC C S F + S + C+C+ G +GK C++C GFY G C C+C G+ NC+ + G C C P +G +CD+CAPN+
Sbjct: 894 CHVNGSVSTSCNTETGQCQ-CRNNVIGRQCDECLPQTF-GLQSAQGCVPCNCNS----FGSKSFDCDDSGQ--CRCQPGVTGKKCDHCDHGFYDFQEGG--CTPCECPHVGN---NCDADTGHCVCPPNTIGEQCDKCAPNH 1022 HSP 10 Score: 57.3806 bits (137), Expect = 6.089e-7 Identity = 54/185 (29.19%), Postives = 70/185 (37.84%), Query Frame = 2
Query: 3218 CVPCNC-GRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
CVPC C G + CD ++ C+ C N A C +C G++G+ C PC CP N +F+ T C + G+ C AC + G C+RC GY G P +CQ C C G+ S C TGQC CK G KC C
Sbjct: 1389 CVPCQCNGHSDI---CDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPC------------ACPLLISSN----------NFSPT-------CTLQGTNDYRCT------ACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLS---MICHPTTGQCTCKTGSTGLKCAGC 1532 HSP 11 Score: 56.225 bits (134), Expect = 1.624e-6 Identity = 64/227 (28.19%), Postives = 88/227 (38.77%), Query Frame = 2
Query: 4619 CNCNGY---SKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSA----------------------------HYNF-------AQNCTRIESSLELKCQ-------CRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE----------FGICQCSPGVLGIKCDQCAPN----YVVEKKTCQSCY-----SGCTK-DLLVRI 5104
CNCN + S CD +G C+ C G KC C GFY D C PC+CP H+ + A NC+ SL ++C CR +SG C +C+ G+ P C +C C GSQ C++ G C C V G+ CD+C P+ +V C SCY + CT+ D L+R+
Sbjct: 941 CNCNSFGSKSFDCDD-SGQCR-CQPGVTGKKCDHCDHGFY--DFQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSN-PGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPT----CVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCFGLATHCTEADGLIRM 1158
BLAST of Laminin subunit alpha-2 vs. Ensembl Xenopus
Match: wdcp (WD repeat and coiled coil containing [Source:Xenbase;Acc:XB-GENE-5729803]) HSP 1 Score: 724.546 bits (1869), Expect = 0.000e+0 Identity = 455/1179 (38.59%), Postives = 618/1179 (52.42%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNR--NETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIY--------NRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQK--PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNN-PTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDS---PDVIIKCGDFRLFHSVSIEDRRLQRQVF------IYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITLS-RGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP-RLDY-QMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQES-----CDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSC---TSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYN---------GQVCNNE----------GICVCPPNTIGNLCNKCSEDSWDFN--ETIGCKKCNCY 3514
L NA+IT++ATCG G E FC+LV+ + Q P + C CD + N RHPI A + N WWQSPS+ G++Y V VT+DL+QV+QI YI++K +SPRPGNWILERSLDG + PW ++A T C ++ + A+ S DDE+ CT++YS+ L +GEI SLI GR S + P+ L+ F SARYIR R + TL ADLM L QS Y RY+YS+++I +GG CIC HA C + C C+HNTCG +C+ CCP FNQ+PWK CE CNC+ KS EC Y+Q VA +LS++ +G GGGVCINC NT GINCE C+ FYRP V T+ YPC PC C +G++ E + PG C CK G+ G CD C++GY YP EC+ C CS+ GS T+ C PC CK +V + C CK GYFNL NP+GC+ C+C G+++ C+ KI+D+ GW +T +G P F L+ N D R P VYYW+APP YL G Y + + +++K +DS P++II+ + +S + +Q F I + P ++I + ++ EF+ L V+KLLI+A Y + Q++I L ++TL G+ + +E C CP GF+G SCESC G RRVN L+ G CE C CY H++ C + GEC +C HNT G +C+ C G+YG+ CK C CP +D + C + VC C +G C+RC EGYYG P + SCK CKCNNN + CD TG C C G C C G++GDAL + C+ C+C GS S +C+ TG+C CR N + RQCD C T GL + C+PCNC G S+ F SGQC C V G +C+ C G++++ C C C PH + +CD + G C C N I CDKC H+ ++ T C CNC GSL+ +C+ +TG C C N + CS C +GY +K P C N Q C+ E G C C N G LC++C ++ + +GC C C+
Sbjct: 38 LATNARITTNATCGEKGQETFCKLVEHVPGQ----PLRYSQCRTCDQKSSNPNLRHPISNA-INGKNSWWQSPSIQNGMQYHYVTVTLDLQQVFQIAYIIIKAANSPRPGNWILERSLDGVNFKPWQYYAITESECLTRYKVK------PQPAQASFAKDDEVICTSHYSKIHPLENGEIFTSLIIGRPSAEDPS-------PE-LLEFTSARYIRLRLQRIRTLNADLM----MLSQSTYKDIDPIVTRRYFYSIKDISVGGMCICYGHARSCP--FNLATKKSSCECEHNTCGDSCDRCCPGFNQKPWKAGTFLLKHECEPCNCHWKSEECYYDQQVADKRLSLDILGNYSGGGVCINCTSNTAGINCETCVDRFYRPKGVSPTQPYPCQPCSCDPIGSLHDICVKDEKQAPRGMLPGSCHCKSGFAGERCDKCAVGYIGYP--ECVRCNCSLIGS--TNKDPCVDPCICKDNVEGQN-CDQCKLGYFNLQKDNPQGCDQCFCSGVSSICTGSRLAYTKITDMNGWYVTDLSGYTRVAPAKDGFDGPHQLSITNR--DARAQGLPEVYYWSAPPTYL-----PVGGHLSYTISYD--ASMKTDSVDSTNYPNIIIEGNGIK----ISSQQETIQLGPFEEITETIVLHPDSFRI--YGTEFQVSRREFMMVLANVNKLLIRATYSNGQEAIYRLSSVTLEVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPGNRRVNGTLFGGSCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQ-SAQGCVPCNCNSFG---SKSFDCDDSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPCEC-PH-VGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGY--------RKYPTCVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCF 1157 HSP 2 Score: 243.047 bits (619), Expect = 4.294e-63 Identity = 165/461 (35.79%), Postives = 225/461 (48.81%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEG-------YYGNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYG---DALTKSCKKCDCDSLGSTSEN------CDKLTG-KCD-CRTNFVNRQCDSCTSGLYLKDKK----CIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGT------KSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEG---GCFSCFCFGVSEKC 3832
+C SG C+ C+ G +G SC+ C+C + + C VTGEC C +NT G C C GFYG + + CK C C L S N DK G CD CR + ++C+ C G Y + + C PC C +S+ C++ +G C C + G C KC EGYF C+ C C + ++S C+ G+C+C +NVI R CD+C + + CVPCNC GS + CD ++G C+C V + C CD G++ GC C + G C+ + G CVCPPNTIG C+KC+ + W ++ GCK CNC GS S CN +G C C+ F G KC C GY +P C C C G+ + E C + TGQC CK NV G C C+ TF G + G GC SC+CFG++ C
Sbjct: 713 QCPSGFSGTSCESCLPGNRRVNGTLFGG-----SCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSC-PLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQSAQGCVPCNCNSFGSKSFDCD-DSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPTCVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTF---GLHVGNPLGCSSCYCFGLATHC 1163 HSP 3 Score: 232.261 bits (591), Expect = 7.030e-60 Identity = 199/700 (28.43%), Postives = 286/700 (40.86%), Query Frame = 2
Query: 1007 VAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNR---KSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGT-----IGSCVSNGEDIGDQ-----------------------KPGD-----------CICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSV---------RTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSS--------LSAKN----LKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCG---DFRLF--HSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRL--DYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCN---NNQESCDPVTGECRCSYNTMGTECQLC--KHGFYGDALTKSCKKCDCDSLGSTSENCDKL 2848
+C C++N G C+ C P + C CNCN KS +C + G C CQ G C+ C GFY + CTPC+C +G G CV IG+Q PG C C+ + G C C +GY +YP C+ C C + GS + S CNCK++V LC CK F L NP GC CYCFG+ C+ L+ K L + DL TT G P+ + E+ + + +YW P + G +T+YG +Y + E + S + P VII+ G + R+ + + ++ +L R I + +WK + ++E+F+ L + +LIKA Y S + + I+L E + P IEKC CP G++GLSCE+C G+ RV G C C C HS+ CD + CQ C HNTAG HCE CA G+YG + + + CK C CP L + C+ + +Y C C G +C+RC GY GNP SC+ C+CN + C P TG+C C + G +C C +H G C CD + G ++ ++L
Sbjct: 922 TGQCQCRNNVIGRQCDECLP--QTFGLQSAQGCVPCNCNSFGSKSFDC-------------------DDSGQC-RCQPGVTGKKCDHCDHGFYD------FQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPT--CVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGV-LCDRCKPSTFGLHVGNPLGCSSCYCFGLATHCTEADGLIRMWLTLKPDQTVLPLVDLTQQHRTT-KGVTYQHPEIVAMIDQVKEDLHAEP-------FYWRLPQQFEGRKLTAYGGKLKYAIYFEARDETGPSTYE-PQVIIRGGPHANHRIIVRNMAAPQNGQLTRHD-IEMTEHDWKYLGSVDGRPVSREDFMDVLYDIQYILIKAAYGSVLRQTRISEISLEVAEEGTSFTISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGRRTGPTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRG-SPDDCKPCACPLLISSNNFSPTCTLQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLSMICHPTTGQCTCKTGSTGLKCAGCTDRHAREGPV----CVFCDDECTGLLLDDLEQL 1575 HSP 4 Score: 176.407 bits (446), Expect = 6.974e-43 Identity = 192/721 (26.63%), Postives = 283/721 (39.25%), Query Frame = 2
Query: 3086 CLCSPHALSADCDLNNGK--CKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICV-CPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI---------LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNA---NYPRRFGWPLTMFRPIYVNV---MKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINS---NLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSA--HYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEFGICQ--------CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
C+C HA S +L K C+C N CD+C N W T +C PCNC ++ C Q VA + S LD+ G Y+G G+C+ C NT G C C + + + C+ C+C GS +IC K + G C CK+ F G +CD+C GY G+P C C+C+ G+ T D C CK NV G C C+ G F GC CFC GVS C +N V + + K+ + S + +A P + W P Y+ V + S+D S+ S+ + + G +I Q E G F +I + ++ DS I ++R FMM L NV L ++ + ++ +T +V + GK +VE C C + G C++ + G N +G++ G + + C C G++ C VTG+C +C N G C C GFY N + + C+PC CP NF+ C ++ + L C CR GY+G+ CE C +G+YG P + G CK C CN +L+ + G C C PG+ G C++CA Y +E + CQSC
Sbjct: 279 CICYGHARSCPFNLATKKSSCECEHNTCGDSCDRCCPGFNQKPWKAGTFLLKHECEPCNC---------HWKSEECYYDQQVADKRLS--------LDILGN------YSGG-----GVCINCTSNTAGINCETCVDRFYRPKGVSPTQPYPCQPCSCDPIGSLHDICVKDEKQAPRGMLPGSCHCKSGFAGERCDKCAVGYIGYPECVRCNCSLIGS-----TNKDPCVDPCICKDNVEGQNCDQCKLGYFNLQKDNPQGCDQCFCSGVSSICTGSRLAYTKITDMNGWYVTDLSGYTRVAPAKDGFDGPHQLSITNRDARAQGLPEVYYWSAP---PTYLPVGGHLSYTISYDASMKTDSVDSTNYPNIIIEGNGIKISSQQETIQL---GPF-----------------------------------EEITETIVLHPDSFRIYGTEFQVSRREFMMVLANVNKLLIRATYSNGQEAIYRLSSVTLEVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPG---------NRRVNGTLFGG-----SCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTC-HLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCN----NNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSC 906 HSP 5 Score: 151.369 bits (381), Expect = 2.591e-35 Identity = 182/743 (24.50%), Postives = 316/743 (42.53%), Query Frame = 2
Query: 4313 LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVME--TTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINF--------KWKTLPSTLDLRLNKIN--ISIQMSSELLNSI--NKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNK----VLEDLRSTFTDINETISGHEQLGISNELIKNATAYLET-IKNIYKEMPNITL--AASVLKDVLKYVDELNQIIK--YTQSHIQSDQINN-------------------LKILKNKTIMADEINTNNKEYLNTMSTK-------KKEHELYI--------------ILINERMKDLQKIFRKMTKQR----DNLRKKIQKLDAISKSFQDEKDLIS-INVREIEDLM----KNLSSNHLVS-------KSLI-IPDTLKKHVEQQLNKSSNLIK------------KLTKDFNFLHNAEQAKEAYSNITRDILAVD--------------YAINQTKKELDF--NRSNMNIDSLKKLLAEFNSNKSEIVENIKNID 6199
++R+ FM L ++ + +K V +I I+ +V E T+ I R +EKC C G C+ G+Y+ P RTG L C CNG+S +CD + CQ C N G+ C+ C GFY + D C+PC CP + NF+ CT ++ + + +C C +GY G+ CE C G+ GNP + G C+ C+CN GS S+ C+ G C C G G+KC C + E C C CT LL ++ LN+ V++ +KTL T + ++ +S Q + E L + N L E+ + K+ D ++ + E+ R + V E LR++ ++ + +E LGI N+ + + L+ I ++ E+ +L + D LK + L +K + ++H +++ +NN L+ + K AD+++T N++ + + K KKE E + +INE + D+ K +T+Q +NL ++I + D + Q E I+ + D + KNLS N V+ K LI + K + + N++ +L+ + K F L +A++ + D+ + A+N T +L N + + I + K + N S+++E +K+++
Sbjct: 1310 VSREDFMDVLYDIQYILIKAAYGSV-LRQTRISEISLEVAEEGTSFTISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGR------RTG-PTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPCACPLLISSNNFSPTCT-LQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLSMICHPTTGQCTCKTGSTGLKCAGCTDRHAREGPVCVFCDDECTGLLLDDLEQLNQMVMSVNLSGPLPPPYKTL-FTFGNQTQELKHLLSPQRAPERLLQLAQNNQDTLVIEMDELLNRAAKVTADGEQTGKDAERTNERAKQLEDFVKETLRASKDIYDKAVKLNETLGIPNKTLDKSFQELQKDIDSMMAELRRKSLRNQKNTADDELKAAEALLNRVKKLFLEAHKKNEDLNNEVKEKLGLFEEKLDDVWDLLREARGKIKAADDLSTVNQKNMTELERKKQGVEKGKKETESVLGDGDSIMTEVNNLAQIINEDLSDMNKELPPLTEQLNEKINNLTQEIARRDLPEQILQAENQAAQLIDSSAVLDGILAEAKNLSFNATVAFNAYSNIKDLIDDAEDTAKEAKAKANEAIDLVTGPQGSLKDEAKNAIQKSFRVLSDAKRHSGDVKDNENDLAGIKNRLENADEKNQNMLQALNDTFGKLALIPNDTLVKIQTAKDKAKQANDTASDVLERLKDLN 2042 HSP 6 Score: 125.946 bits (315), Expect = 1.191e-27 Identity = 107/337 (31.75%), Postives = 139/337 (41.25%), Query Frame = 2
Query: 2951 EIQGRISSEKFCNTTSGQCFCLENVNGDRCEKC------VEGYFNYPNCKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWN----NRTDCVPCNC----GRGSLNPKCDMETGNC----KCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-------------EGIC-VCPPNTIGNLCNKCSEDSW-DFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQ---PCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNH 3850
E+ G SS + C C +G CE C V G +C+ C C H + C G+C C N R CD C Y N DC PC+C + +P C ++ C + C +C GY+G C PC CNN G C VC P G CNKC+E + D E GC+ CNC++NGS S CN +G C C+ G +CD C +G + Q PC+C G+KS + CD +GQC+C+ V G KC C G + EGGC C C V C A H
Sbjct: 694 EVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPGNRRVNGTLFGGSCEPCRCYGH--TDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCK----CNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQSAQGCVPCNCNSFGSKSFD---CDD-SGQCRCQPGVTGKKCDHCDHGFY---DFQEGGCTPCECPHVGNNCDADTGH 1017 HSP 7 Score: 75.0998 bits (183), Expect = 2.750e-12 Identity = 57/185 (30.81%), Postives = 81/185 (43.78%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYY----GNPKKKI-----SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGST---SENCDKLTGKCD-----CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVE 3055
+C H SG+ C+ C G+Y P ++ +C C+CN + + CDP + C+ C +NT G C+ C GFYG CK C C L S+ S C L G D C + + C+ C SG + C C C G +S C+ T+GQC C G +C C +
Sbjct: 1367 ECPHGYSGLSCEACAPGFYRVAPATPGRRTGPTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPCACPLLISSNNFSPTC-TLQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLS--MICHPTTGQCTCKTGSTGLKCAGCTD 1548 HSP 8 Score: 73.1738 bits (178), Expect = 1.144e-11 Identity = 54/160 (33.75%), Postives = 75/160 (46.88%), Query Frame = 2
Query: 4613 KKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCT-RIESSLELKCQCRIGYSGKSCENC-QQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPN-YVVEKKTCQSC 5068
K C CN + CDS TG C C G C C EG++ + C+ C C H N + + + E+ +CQCR G+ C+ C Q F +G C C+CN GS+S +C++ G C+C PGV G KCD C Y ++ C C
Sbjct: 851 KPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARGCQSCNC---HVNGSVSTSCNTETG---QCQCRNNVIGRQCDECLPQTFGLQSAQG--CVPCNCNSFGSKSFDCDDSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPC 1002 HSP 9 Score: 63.929 bits (154), Expect = 6.530e-9 Identity = 51/146 (34.93%), Postives = 73/146 (50.00%), Query Frame = 2
Query: 4613 KKCNC--NG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
+ CNC NG S C++ TG CQ C N G +C C + ++ C PC C S F + S + C+C+ G +GK C++C GFY G C C+C G+ NC+ + G C C P +G +CD+CAPN+
Sbjct: 904 QSCNCHVNGSVSTSCNTETGQCQ-CRNNVIGRQCDECLPQTF-GLQSAQGCVPCNCNS----FGSKSFDCDDSGQ--CRCQPGVTGKKCDHCDHGFYDFQEGG--CTPCECPHVGN---NCDADTGHCVCPPNTIGEQCDKCAPNH 1036 HSP 10 Score: 57.7658 bits (138), Expect = 5.463e-7 Identity = 54/185 (29.19%), Postives = 70/185 (37.84%), Query Frame = 2
Query: 3218 CVPCNC-GRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
CVPC C G + CD ++ C+ C N A C +C G++G+ C PC CP N +F+ T C + G+ C AC + G C+RC GY G P +CQ C C G+ S C TGQC CK G KC C
Sbjct: 1403 CVPCQCNGHSDI---CDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPC------------ACPLLISSN----------NFSPT-------CTLQGTNDYRCT------ACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLS---MICHPTTGQCTCKTGSTGLKCAGC 1546 HSP 11 Score: 56.225 bits (134), Expect = 1.340e-6 Identity = 64/227 (28.19%), Postives = 88/227 (38.77%), Query Frame = 2
Query: 4619 CNCNGY---SKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSA----------------------------HYNF-------AQNCTRIESSLELKCQ-------CRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE----------FGICQCSPGVLGIKCDQCAPN----YVVEKKTCQSCY-----SGCTK-DLLVRI 5104
CNCN + S CD +G C+ C G KC C GFY D C PC+CP H+ + A NC+ SL ++C CR +SG C +C+ G+ P C +C C GSQ C++ G C C V G+ CD+C P+ +V C SCY + CT+ D L+R+
Sbjct: 955 CNCNSFGSKSFDCDD-SGQCR-CQPGVTGKKCDHCDHGFY--DFQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSN-PGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPT----CVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCFGLATHCTEADGLIRM 1172
BLAST of Laminin subunit alpha-2 vs. Ensembl Xenopus
Match: wdcp (WD repeat and coiled coil containing [Source:Xenbase;Acc:XB-GENE-5729803]) HSP 1 Score: 718.383 bits (1853), Expect = 0.000e+0 Identity = 457/1180 (38.73%), Postives = 622/1180 (52.71%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNR--NETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIY--------NRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQK---PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNN-PTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDS---PDVIIKCGDFRLFHSVSIEDRRLQRQVF------IYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITLS-RGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRL--DYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQES-----CDPVTGECR-CSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSC---TSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYN---------GQVCNNE----------GICVCPPNTIGNLCNKCSEDSWDFN--ETIGCKKCNCY 3514
L NA+IT++ATCG G E FC+LV+ + Q P + C CD + N RHPI A + N WWQSPS+ G++Y V VT+DL+QV+QI YI++K +SPRPGNWILERSLDG + PW ++A T C ++ + A+ S DDE+ CT++YS+ L +GEI SLI GR S + P+ L+ F SARYIR R + TL ADLM L QS Y RY+YS+++I +GG CIC HA C + S C C+HNTCG +C+ CCP FNQ+PWK CE CNC+ KS EC Y+Q VA +LS++ +G GGGVCINC NT GINCE C+ FYRP V T+ YPC PC C +G++ CV + + K PG C CK G+ G CD C++GY YP EC+ C CS+ GS T+ C PC CK +V + C CK GYFNL NP+GC+ C+C G+++ C+ KI+D+ GW +T +G P F L+ N D R P VYYW+APP YL + +T+ G Y + + +++K +DS P++II+ + +S + +Q F I + P ++I + ++ EF+ L V+KLLI+A Y + Q++I L ++TL G+ + +E C CP GF+G SCESC G RRVN L+ G CE C CY H++ C + GEC +C HNT G +C+ C G+YG+ CK C CP + C + VC C +G C+RC EGYYG P + SCK CKCNNN + CD TG C C G C C G++GDAL + C+C GS S +C+ TG+C CR N + RQCD C T GL + C+PCNC G S+ F SGQC C V G +C+ C G++++ C C C PH + +CD + G C C N I CDKC H+ ++ T C CNC GSL+ +C+ +TG C C N + CS C +GY +K P C N Q C+ E G C C N G LC++C ++ + +GC C C+
Sbjct: 21 LATNARITTNATCGEKGQETFCKLVEHVPGQ----PLRYSQCRTCDQKSSNPNLRHPISNA-INGKNSWWQSPSIQNGMQYHYVTVTLDLQQVFQIAYIIIKAANSPRPGNWILERSLDGVNFKPWQYYAITESECLTRYKVK------PQPAQASFAKDDEVICTSHYSKIHPLENGEIFTSLIIGRPSAEDPS-------PE-LLEFTSARYIRLRLQRIRTLNADLMM----LSQSTYKDIDPIVTRRYFYSIKDISVGGMCICYGHARSCPFNLATKKS--SCECEHNTCGDSCDRCCPGFNQKPWKAGTFLLKHECEPCNCHWKSEECYYDQQVADKRLSLDILGNYSGGGVCINCTSNTAGINCETCVDRFYRPKGVSPTQPYPCQPCSCDPIGSLHDICVKDEKQAPRGKGMLPGSCHCKSGFAGERCDKCAVGYIGYP--ECVRCNCSLIGS--TNKDPCVDPCICKDNVEGQN-CDQCKLGYFNLQKDNPQGCDQCFCSGVSSICTGSRLAYTKITDMNGWYVTDLSGYTRVAPAKDGFDGPHQLSITNR--DARAQGLPEVYYWSAPPTYLRSKVTAVGGHLSYTISYD--ASMKTDSVDSTNYPNIIIEGNGIK----ISSQQETIQLGPFEEITETIVLHPDSFRI--YGTEFQVSRREFMMVLANVNKLLIRATYSNGQEAIYRLSSVTLEVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPGNRRVNGTLFGGSCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDAL--EARACNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQ-SAQGCVPCNCNSFG---SKSFDCDDSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPCEC-PH-VGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGY--------RKYPTCVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCF 1144 HSP 2 Score: 338.191 bits (866), Expect = 4.573e-92 Identity = 297/1003 (29.61%), Postives = 440/1003 (43.87%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEG-------YYGNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYG---DALTKSCKKCDCDSLGSTSEN------CDKLTG-KCD-CRTNFVNRQCDSCTSGLYLKDKK----CIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF-NYPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGT------KSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI--LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESY-NGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVME--TTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINF--------KWKTLPS----TLDLRLNKINIS-IQMSSELLNSINKFYKLKNEIKNFSI-------QFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRS--TFTDIN 5383
+C SG C+ C+ G +G SC+ C+C + + C VTGEC C +NT G C C GFYG + + CK C C L S N DK G CD CR + ++C+ C G Y + + C PC C +S+ C++ +G C C + G C KC EGYF + + C C + ++S C+ G+C+C +NVI R CD+C + + CVPCNC GS + CD ++G C+C V + C CD G++ GC C + G C+ + G CVCPPNTIG C+KC+ + W ++ GCK CNC GS S CN +G C C+ F G KC C GY +P C C C G+ + E C + TGQC CK NV G C C+ TF GC SC+CFG++ C L V + I K G Y + A + P Y + + F L+ + + + + D Q + + N +R + ++ EH W K++ +V ++R+ FM L ++ + +K V +I I+ +V E T+ I R +EKC C G C+ G+Y+ P RTG L C CNG+S +CD + CQ C N G+ C+ C GFY + D C+PC CP F+ CT ++ + + +C C +GY G+ CE C G+ GNP + G C+ C+CN GS S+ C+ G C C G G+KC C + E C C CT LL ++ LN+ V++ +KTL + T +L++N IS + S SIN ++ E KN S + +KD + ++ K + N+ ++ +R+ +FT I+
Sbjct: 703 QCPSGFSGTSCESCLPGNRRVNGTLFGG-----SCEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSC-PLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARACNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQSAQGCVPCNCNSFGSKSFDCD-DSGQCRCQPGVTGKKCDHCDHGFYDFQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPTCVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGVLCDRCKPSTFGLHVGNPLGCSSCYCFGLATHCTEADGLIRMWVSRKQLSHGIL--KGGTTKGVTYQHPEIVAMIDQV--KEDLHAEPFYWRLPQQFEGRKLTAYGGKLKYAIYFEARDETGPSTYEPQVIIRGGPHANHRIIVRNMAAPQNGQLTRHDIEMTEHD----W-----------KYL----GSVDGRPVSREDFMDVLYDIQYILIKAAYGSVLRQ-TRISEISLEVAEEGTSFTISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGR------RTG-PTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRGSPDDCKPCACPF----FSPTCT-LQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLSMICHPTTGQCTCKTGSTGLKCAGCTDRHAREGPVCVFCDDECTGLLLDDLEQLNQMVMSVNLSGPLPPPYKTLFTFGNQTQELKVNYCFISEVTFRSNCYTSIN-LCRILAEAKNLSFNATVAFNAYSNIKDLIDDAEDTAKEAKAKANEAIDLVRTKKSFTKIH 1661 HSP 3 Score: 230.335 bits (586), Expect = 2.318e-59 Identity = 198/694 (28.53%), Postives = 284/694 (40.92%), Query Frame = 2
Query: 1007 VAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNR---KSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGT-----IGSCVSNGEDIGDQ-----------------------KPGD-----------CICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSV---------RTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSS--------LSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCG---DFRLF--HSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCN---NNQESCDPVTGECRCSYNTMGTECQLC--KHGFYGDALTKSCKKCDCDSLGSTSENCDKL 2848
+C C++N G C+ C P + C CNCN KS +C + G C CQ G C+ C GFY + CTPC+C +G G CV IG+Q PG C C+ + G C C +GY +YP C+ C C + GS + S CNCK++V LC CK F L NP GC CYCFG+ C+ +S K L LKG T G P+ + E+ + + +YW P + G +T+YG +Y + E + S + P VII+ G + R+ + + ++ +L R I + +WK + ++E+F+ L + +LIKA Y S + + I+L E + P IEKC CP G++GLSCE+C G+ RV G C C C HS+ CD + CQ C HNTAG HCE CA G+YG + + + CK C CP + C+ + +Y C C G +C+RC GY GNP SC+ C+CN + C P TG+C C + G +C C +H G C CD + G ++ ++L
Sbjct: 909 TGQCQCRNNVIGRQCDECLP--QTFGLQSAQGCVPCNCNSFGSKSFDC-------------------DDSGQC-RCQPGVTGKKCDHCDHGFYD------FQEGGCTPCECPHVGNNCDADTGHCVCPPNTIGEQCDKCAPNHWGYSIITGCKACNCSNPGSLSMQCNSDTGCCFCRPNFSGDKCSSCRLGYRKYPT--CVNCGCMLAGSQPQTCDQETEKCSCAEQTGQCNCKINVEGV-LCDRCKPSTFGLHVGNPLGCSSCYCFGLATHCTEADGLIRMWVSRKQLSHGILKG---GTTKGVTYQHPEIVAMIDQVKEDLHAEP-------FYWRLPQQFEGRKLTAYGGKLKYAIYFEARDETGPSTYE-PQVIIRGGPHANHRIIVRNMAAPQNGQLTRHD-IEMTEHDWKYLGSVDGRPVSREDFMDVLYDIQYILIKAAYGSVLRQTRISEISLEVAEEGTSFTISPRAYLIEKCECPHGYSGLSCEACAPGFYRVAPATPGRRTGPTLGTCVPCQCNGHSDICDPQSSVCQACKHNTAGDHCERCAPGFYGIVRG-SPDDCKPCACPFF----SPTCTLQGTNDYRCTACPLGYEGQYCERCASGYTGNPGVPGGSCQECECNPYGSLSMICHPTTGQCTCKTGSTGLKCAGCTDRHAREGPV----CVFCDDECTGLLLDDLEQL 1550 HSP 4 Score: 167.162 bits (422), Expect = 4.093e-40 Identity = 189/722 (26.18%), Postives = 280/722 (38.78%), Query Frame = 2
Query: 3086 CLCSPHALSADCDLNNGK--CKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICV-CPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK-----------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI---------LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNA---NYPRRFGW--PLTMFRPIYVNV---MKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSN---LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSA--HYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEFGICQ--------CSPGVLGIKCDQCAPNYVVEKKTCQSC 5068
C+C HA S +L K C+C N CD+C N W T +C PCNC ++ C Q VA + S LD+ G Y+G G+C+ C NT G C C + + + C+ C+C GS +IC K + G C CK+ F G +CD+C GY G+P C C+C+ G+ T D C CK NV G C C+ G F GC CFC GVS C +N V + + K+ + S + +A P + W P T R V + S+D S+ S+ + + G +I Q E G F +I + ++ DS I ++R FMM L NV L ++ + ++ +T +V + GK +VE C C + G C++ + G N +G++ G+ + C C G++ C VTG+C +C N G C C GFY N + + C+PC CP NF+ C ++ + L C CR GY+G+ CE C +G+YG P + G CK C CN +L+ + G C C PG+ G C++CA Y + ++C
Sbjct: 262 CICYGHARSCPFNLATKKSSCECEHNTCGDSCDRCCPGFNQKPWKAGTFLLKHECEPCNC---------HWKSEECYYDQQVADKRLS--------LDILGN------YSGG-----GVCINCTSNTAGINCETCVDRFYRPKGVSPTQPYPCQPCSCDPIGSLHDICVKDEKQAPRGKGMLPGSCHCKSGFAGERCDKCAVGYIGYPECVRCNCSLIGS-----TNKDPCVDPCICKDNVEGQNCDQCKLGYFNLQKDNPQGCDQCFCSGVSSICTGSRLAYTKITDMNGWYVTDLSGYTRVAPAKDGFDGPHQLSITNRDARAQGLPEVYYWSAPPTYLRSKVTAVGGHLSYTISYDASMKTDSVDSTNYPNIIIEGNGIKISSQQETIQL---GPF-----------------------------------EEITETIVLHPDSFRIYGTEFQVSRREFMMVLANVNKLLIRATYSNGQEAIYRLSSVTLEVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPG---------NRRVNGTLFGGS-----CEPCRCYGHTDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTC-HLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCKCN----NNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGYFGDALEARAC 893 HSP 5 Score: 116.701 bits (291), Expect = 6.109e-25 Identity = 104/336 (30.95%), Postives = 137/336 (40.77%), Query Frame = 2
Query: 2951 EIQGRISSEKFCNTTSGQCFCLENVNGDRCEKC------VEGYFNYPNCKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWN----NRTDCVPCNC----GRGSLNPKCDMETGNC----KCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-------------EGIC-VCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN---CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNH 3850
E+ G SS + C C +G CE C V G +C+ C C H + C G+C C N R CD C Y N DC PC+C + +P C ++ C + C +C GY+G C PC CNN G C VC P G CNKC+E F + + + CNC++NGS S CN +G C C+ G +CD C +G + C PC+C G+KS + CD +GQC+C+ V G KC C G + EGGC C C V C A H
Sbjct: 684 EVAGDPSSSGKTAFSVELCQCPSGFSGTSCESCLPGNRRVNGTLFGGSCEPCRCYGH--TDRCHDVTGECLDCKHNTYGRYCDYCLPGFYGNPSEGTAEDCKPCSCPLTIDSNNFSPTCHLDKTRGLVCDSCRPGYTGQRCERCAEGYYGQPSEPGGSCKPCK----CNNNLDLSTPGCCDSQTGACLVCKPGITGQYCNKCAEGY--FGDALEARACNCHVNGSVSTSCNTETGQCQCRNNVIGRQCDECLPQTFGLQSAQGCVPCNCNSFGSKSFD---CDD-SGQCRCQPGVTGKKCDHCDHGFYDFQ---EGGCTPCECPHVGNNCDADTGH 1004
BLAST of Laminin subunit alpha-2 vs. Ensembl Mouse
Match: Lama1 (laminin, alpha 1 [Source:MGI Symbol;Acc:MGI:99892]) HSP 1 Score: 747.273 bits (1928), Expect = 0.000e+0 Identity = 441/1186 (37.18%), Postives = 640/1186 (53.96%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT--HKGY--LEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGE--DIGDQK-PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTF-TGEKIALPK--FTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQ-STLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQ--RQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITL---SRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP-RLDY-QMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK--KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYW--NNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC---YNGQV---CNNE----------GICVCPPNTIGNLCNKCSEDSWDF--NETIGCKKCNCYINGSYSNICNKISG 3556
L NA I+++ATCG GPE+FC+LV+ + + P H C VCD + N +RHPI A D N WWQSPS+ G +Y V VT+DL+QV+Q+ YI++K ++PRPGNWILERS+DG + PW ++A + C Y + R+ P P+ AD+E+ CT+YYS+ L GEI SLINGR S + PQ L+ F SARYIR R + TL ADLMT H+ L+ + RYYYS+++I +GG CIC HA C + +C C+HNTCG +C+ CCP ++Q+PW+P + CEECNC+ K+ +C Y+ +VA + S+N G+ GGGVC+NC +NT GINCE CI +YRP V + +PC PC C +G++ S C+ + D+ + K PG C C+KGY G CD C GY +PN C+PC C GS+ C PC CK +V + C CK G++NL NPEGC C+CFG++ C SL+ +++++ GW +T + KI + + ++ N+ + +R + YYW AP YLGN +T++G +Y V ++ T+ L+ D+IIK + S E LQ + F ++ + +++ ++ L V LLI+A Y S + ++ L +++L S + LA+ +E C CP G+ G SCE+C GY RV+ +L+ G C+ C C+ H++ CD ++G C CTHNT G HCE C G+YG C+ C CP +D + C E VC +C SG C+RC +GYYGNP +C C C+ N + CD VTGEC +C +NT G C+ C GFYGDA+T K+C+ CDC GS S C TG CDC+ + +QCD C SG Y D C+PCNC ++G +S C T GQC C V+G +C++C G++ + + C C C+ +CD +G+C C + C++CE YW + C CNC GS + +CD+ +G+C C + + C +C +GY C PC G + C+ E G C C N +G C+KC ++ + GC C C+ S +C+++ G
Sbjct: 36 LATNAHISANATCGEKGPEMFCKLVEHVPGR----PVRHAQCRVCDGNSTNPRERHPISHAI-DGTNNWWQSPSIQNGREYHWVTVTLDLRQVFQVAYIIIKAANAPRPGNWILERSVDGVKFKPWQYYAVSDTECLTRYKITPRRGP---------PTYRADNEVICTSYYSKLVPLEHGEIHTSLINGRPSADDPS-------PQ-LLEFTSARYIRLRLQRIRTLNADLMTLSHRDLRDLDPIVTRRYYYSIKDISVGGMCICYGHASSCP--WDEEAKQLQCQCEHNTCGESCDRCCPGYHQQPWRPGTISSGNECEECNCHNKAKDCYYDSSVAKERRSLNTAGQYSGGGVCVNCSQNTTGINCETCIDQYYRPHKVSPYDDHPCRPCNCDPVGSLSSVCIKDDRHADLANGKWPGQCPCRKGYAGDKCDRCQFGYRGFPN--CIPCDCRTVGSLNED--PCIEPCLCKKNVEGKN-CDRCKPGFYNLKERNPEGCSECFCFGVSGVCDSLTWSISQVTNMSGWLVTDLMSTNKIRSQQDVLGGHRQISINNTAVMQRLT--STYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETVDSDLMSHADIIIKGNGLTI--STRAEGLSLQPYEEYFNVVRLVPENFRDFDTRREIDRDQLMTVLANVTHLLIRANYNSAKMALYRLDSVSLDIASPNAIDLAV--AADVEHCECPQGYTGTSCEACLPGYYRVDGILFGGICQPCECHGHASECD-IHGICSVCTHNTTGDHCEQCLPGFYGTPSRGTPGDCQPCACPLSIDSNNFSPTCHLTDGEEVVCDQCAPGYSGSWCERCADGYYGNPTVPGGTCVPCNCSGNVDPLEAGHCDSVTGECLKCLWNTDGAHCERCADGFYGDAVTAKNCRACDCHENGSLSGICHLETGLCDCKPHVTGQQCDQCLSGYYGLDTGLGCVPCNCSVEGSVSDN--C-TEEGQCHCGPGVSGKQCDRCSHGFYAFQDGGCTPCDCA--HTQNNCDPASGECLCPPHTQGLKCEECEEA-YWGLDPEQGCQACNCSAVGSTSAQCDVLSGHCPCKKGFGGQSCHQCSLGYRSF-----PDCVPCGCDLRGTLPDTCDLEQGLCSCSEDGGTCSCKENAVGPQCSKCQAGTFALRGDNPQGCSPCFCF---GLSQLCSELEG 1171 HSP 2 Score: 406.757 bits (1044), Expect = 5.891e-113 Identity = 416/1709 (24.34%), Postives = 689/1709 (40.32%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPKKKIS--CKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCD-SLGST--SENCDKLTGK---CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNE------LTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYE----YHIFDEKN--SLESGKDYST--IHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDL-----SLFYRSIFMNVSVDCLDN-------KCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIK-IKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP-SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTL-PSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKV----------LEDLRSTFTDINETISGHEQ---------LGISNELIKNATAYLETIK--NIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMA---DEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQK----LDAI---SKSFQD-------EKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNIT-----RDILAVDYAINQTKKELD----FNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSE----------LIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTG-KFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGF-------PSSKLLRSLKLEGRLISDLKE-NYDNYFYK-----GCLFGVKLMNTEFGL--QDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSN 7300
+C +G C+ C+ GYY C+ C+C+ + CD + G C C++NT G C+ C GFYG C+ C C S+ S S C G+ CD C + C+ C G Y + C+PCNC C++ +G+C CL N +G CE+C +G++ NC+ C C + +LS C L G C C +V + CD+C + +Y + CVPCNC GS++ C E G C C V+ + C +C G++ GC C + C+ G C+CPP+T G C +C E W + GC+ CNC GS S C+ +SG C CK F G C +C+ GY FP+C PC C GT + L C + G C CK+N +G +C C+ GTF G GC CFCFG+S+ C + + ++ H+ + N G + L+A R+ + P Y + K F L L Y F + N K GR + ++M++ G+R D+ K W +Y N + +K + D FM L N+ + +K Y + I I + + A G+ +E C+C T G+ CQ+ GYY+E LP S G +L CNCN +S VCD TG C +C + G+ C+LC G+Y C PC CP ++F+ C +E + +C C GY G+ CE C G++GNP G C++CDCN QGS +C+ G C C PG G+ C++C P +++ + C SC C LL +D++ + V++ + P+ + N N + L+ K + + +++ + Q + ++ + +K+ + +E L + +I E ++ Q + + +N ++ L IK N + N TL KD+L + Q Q Q LK LK + E +E L +K +E L ++L+ +K+ Q+ ++ ++++ + I K +DA + + QD +D + + R+I + +L ++ + ++H + +++ L + L N NA A +SNI ++LA D K +L +R + + L E S + + +D E + L Q++ N K N + + E P G + R L+ E +L ++ + N + D + ++ ++ + + K+KD+ L +RL+ + + E L+ A + V I+ V R + Y P +++ + + + +LF Y S S A + R + F +D G+ +E +N + I IT + ++ S V TS + + I T +FVGG P+ K+ G + K NY + GC + ++ F +E+ R T++ +L F+PN + +L SN
Sbjct: 717 ECPQGYTGTSCEACLPGYYRVDGILFGGICQPCECHGHASECD-IHGICSVCTHNTTGDHCEQCLPGFYGTPSRGTPGDCQPCACPLSIDSNNFSPTCHLTDGEEVVCDQCAPGYSGSWCERCADGYYGNPTVPGGTCVPCNCSGNVDPLEAGHCDSVTGECLKCLWNTDGAHCERCADGFYGDAVTAKNCRACDCHENGSLSGICHLETGLCDCKPHVTGQQCDQCLSGYYGLDTGLGCVPCNCSVEGSVSDNC-TEEGQCHCGPGVSGKQCDRCSHGFYAFQDGGCTPCDCAHTQNNCDPASGECLCPPHTQGLKCEECEEAYWGLDPEQGCQACNCSAVGSTSAQCDVLSGHCPCKKGFGGQSCHQCSLGYRSFPDCVPCGCDLRGTLPDTCDLEQGLCSCSEDGGTCSCKENAVGPQCSKCQAGTFALRGDNPQGCSPCFCFGLSQLCSELEGYVRTLITLASDQPLLHVVSQSNLKGTIEGVHFQPPDTLLDAEAVRQHIYA----EPFYWRLPKQFQGDQLLAYGGKLQYSVAFYSTLGTGTSNYEPQVLIKGGRA--RKHVIYMDAPAPENGVR--------------QDYEVRMKEEFW---KYFNSVSEKHVTHSD------------FMSVLSNIDYILIKASYGQGLQQSRIANISMEVGRKAVELPAEGEAALLLELCVCPPGTAGHSCQDCAPGYYRE--KLPESGGRGP----RPLLAPCVPCNCNNHSDVCDPETGKCLSCRDHTSGDHCELCASGYYGKVTGLPGDCTPCTCPHHPPFSFSPTCV-VEGDSDFRCNACLPGYEGQYCERCSAGYHGNPRAAGGSCQTCDCNPQGSVHSDCDRASGQCVCKPGATGLHCEKCLPRHILMESDCVSCDDDCVGPLLNDLDSVGDAVLSLNLTGVSPAPYGILENLENTTKYFQRYLIKENAKKIRAEIQLEGIAEQTENLQKELTRVLARHQKVNAEMERTSNGTQALATFIEQLHANIKEITEKVATLNQTARKDFQPPVSALQSMHQNISSLLGLIKERNFTEMQQNATLELKAAKDLLSRI----------QKRFQKPQ-EKLKALKEANSLLSNHSEKLQAAEELLKEAGSKTQESNLLLLLVKANLKEFQEKKLRVQEEQNVTSELIAKGREWVDAAGTHTAAAQDTLTQLEHHRDELLLWARKIRSHVDDLVMQMSKRRARDLVHRAEQHASELQSRAGALDRDLENVRNVSLNATSAAHVHSNIQTLTEEAEMLAADAHKTANKTDLISESLASRGKAVLQRSSRFLKESVSTRRKQQGITMKLD-ELKNLTSQFQESMDNIMKQANDSLAMLRESPGGMREKGRKARELAAAANESAVKTLEDVLALSLRVFN-------TSEDLSRVNATVQETNDLLHNSTMTTLLAGRKMKDMEMQANLLLDRLKPLKTLEENLSRNLSEIKLLISRARKQVASIKVAVSADRDCIRAYQPQTSSTNYNTLILNVKTQEPDNLLF-YLGSSSSSDFLAVEMRR-GKVAFLWDLGSGSTRLEFPEVSINNNRWHSIYITRFGNMGSLSVKEASAAENPPVRTSK-SPGPSKVLDINNSTLMFVGGLGGQIKKSPAVKVTHFKGCMGEAFLNGKSIGLWNYIEREGKCNGCFGSSQNEDSSFHFDGSGYAMVEKTLRPTVTQIVILFS----TFSPNGLLFYLASN 2355 HSP 3 Score: 235.728 bits (600), Expect = 4.381e-61 Identity = 185/670 (27.61%), Postives = 276/670 (41.19%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLG-TIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSV------RTSFYRCKP---PCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVY----YWTAPPPYLGNWITSYGTIFRYVVKIELQSTL-KGSLIDSPDVIIKCGDFR---LFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLAL----DEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQ--------CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP-RLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLC--KHGFYGDALTKSCKKCDCDSLGSTSENCDKL 2848
C C+ D E G +C+C G C+ C F C C+C N C G C+ C +T+G+ CE+C + + W + + C C C +G T C D G C CKKG+ G +C CS+GY +P +C+PC C + G++ C C+CK + C C+ G F L NP+GC PC+CFG++ CS L + L + + L + ++ +D +Y YW P + G+ + +YG +Y V STL G+ P V+IK G R ++ + +++ + +K WK +K T +F+S L +D +LIKA Y + + NI++ G + L + +E C CPPG G SC+ C GY R G+ C C C NHS+ CD G+C +C +T+G HCE CASGYYG C C CP + + C ++ C+ CL G +C+RC GY+GNP+ SC+ C CN CD +G+C C G C+ C +H + C CD D +G + D +
Sbjct: 958 CNCSVEGSVSDNCTEEG----QCHCGPGVSGKQCDRCSHGFYAFQ---DGGCTPCDCAHTQNNCD------------------PASGECL-CPPHTQGLKCEECEEAY---WGLDPEQ--GCQACNCSAVGSTSAQC--------DVLSGHCPCKKGFGGQSCHQCSLGYRSFP--DCVPCGCDLRGTLPDTCDLEQGLCSCSEDGGTCSCKENAVGPQ-CSKCQAGTFALRGDNPQGCSPCFCFGLSQLCSELEGYVRTLITLASDQPLLHVVSQSNLKGTIEGVHFQPPDTLLDAEAVRQHIYAEPFYWRLPKQFQGDQLLAYGGKLQYSVA--FYSTLGTGTSNYEPQVLIKGGRARKHVIYMDAPAPENGVRQDYEVRMKEEFWKYFNSVSEKHVTHSDFMSVLSNIDYILIKASYGQGLQQSRIANISMEVGRKAVELPAEGEAALLLELCVCPPGTAGHSCQDCAPGYYREKLPESGGRGPRPLLAPCVPCNCNNHSDVCDPETGKCLSCRDHTSGDHCELCASGYYGKVTGLP-GDCTPCTCPHHPPFSFSPTCVVEGDSDFRCNACLPGYEGQYCERCSAGYHGNPRAAGGSCQTCDCNPQGSVHSDCDRASGQCVCKPGATGLHCEKCLPRHIL----MESDCVSCDDDCVGPLLNDLDSV 1578 HSP 4 Score: 152.91 bits (385), Expect = 6.806e-36 Identity = 185/749 (24.70%), Postives = 282/749 (37.65%), Query Frame = 2
Query: 3086 CLCSPHALSA--DCDLNNGKCKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIG-----CKKCNCYINGSYSNIC-----------NKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI---------LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTA-VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILIN--LKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPSA--HYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKG---IPC--------------------------------------------------KSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
C+C HA S D + +C+C N CD+C + W T +C CNC + +C +VA+ S G + G VC N C NT G C C + + ++ C+ CNC GS S++C K G C C+ + G KCDRC GY GFPNC PC C G+ + D C CK+NV G C C+ G + GC CFCFGVS C ++ ++ +V + + I +++ L G +I+ A R + Y + + + L+ F + VS D ++ ++ ++ NGL S + + E+ + V +++ + I + + +N+T ++ N S K+ LY ++ ++ + + AI V A VE C C G C+ + GYY+ + IL + C C+G++ CD + G C C N G+ C+ C GFY C+PC CP + NF+ C + E+ C QC GYSG CE C G+YGNP + G +PC ++CDC++ GS S C+ E G+C C P V G +CDQC Y
Sbjct: 277 CICYGHASSCPWDEEAKQLQCQCEHNTCGESCDRCCPGYHQQPWRPGTISSGNECEECNCHN---------KAKDCYYDSSVAKERRSLNTAGQYS-------------GGGVCVN-----CSQNTTGINCETCIDQYYRPHKVSPYDDHPCRPCNCDPVGSLSSVCIKDDRHADLANGKWPGQCPCRKGYAGDKCDRCQFGYRGFPNCIPCDCRTVGSLNE-----DPCIEPCLCKKNVEGKNCDRCKPGFYNLKERNPEGCSECFCFGVSGVCDSLTWSISQVTNMSGWLVTDLMSTNKIRSQQDVL-GGHRQISINNTAVMQR-------LTSTYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETVDSDLMSHADIIIKG-NGL-------TISTRAEGLSLQPYEEYFNVVRLVPENFRDFDTRREIDRDQLMTVLANVTH---LLIRANYNSAKMALY---------RLDSVSLDIA-SPNAIDLAVAADVEHCECPQGYTGTSCEACLPGYYRV----------------DGILFGGICQPCECHGHASECD-IHGICSVCTHNTTGDHCEQCLPGFYGTPSRGTPGDCQPCACPLSIDSNNFSPTC-HLTDGEEVVCDQCAPGYSGSWCERCADGYYGNPTVPGGTCVPCNCSGNVDPLEAGHCDSVTGECLKCLWNTDGAHCERCADGFYGDAVTAKNCRACDCHENGSLSGICHLETGLCDCKPHVTGQQCDQCLSGY 946
BLAST of Laminin subunit alpha-2 vs. Ensembl Mouse
Match: Lama2 (laminin, alpha 2 [Source:MGI Symbol;Acc:MGI:99912]) HSP 1 Score: 725.317 bits (1871), Expect = 0.000e+0 Identity = 443/1172 (37.80%), Postives = 628/1172 (53.58%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLM--THKG--YLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQ--KPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFT---SFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSL-IDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAA----------TKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDE--MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQE-----SCDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSC---TSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV-TGCKKCPPCYNGQVCNNE----------GICVCPPNTIGNLCNKCSEDSW--DFNETIGCKKCNCY 3514
L NA IT++ATCG GPE++C+LV+ + Q + NP C +C+ + N +RHPI A D N WWQSPS+ G++Y V +T+DL+QV+QI Y+++K +SPRPGNWILERSLD Y PW + A T C YN++ R P PS DDE+ CT++YS+ L +GEI ISLINGR S + P+ L+ F SARYIR RF + TL ADLM HK ++ + RYYYS+++I +GG CIC HA C ++ + ++C C+HNTCG +C+ CCP F+Q+PW+ CE CNC+ K+ EC Y++TVA+ LS+N G+ GGGVCINC NT GINCE C+ GF+RP V PC PC C G++ E + KPG C CK G+ GVNCD C GY YP +C PC CS GS T+ C PC+CK +V +D C CK+G+FNL N +GCE C+C G++N+C S I D++GW LT +G P+ S Q ++ NS + R + YYW+APPPYLGN + + G + + +L+ + I +I + D R+ S + ++ +Y++P+E I +++ + A T+++F+ L ++++L++ Y +I L+ V D +E C CPPG++G SCE+C +RRVN ++ G CE C C+ H+ CD + GEC NC +T G +C +C G+YGD + C+ C CP + C S +C +C +G C+RC EGY+G P SC+ C+CN+N + SCD ++G C C T G C+LC G++GDA+ K+C+ C C+ GS SE C TG+C+CR N R CD C T GL L + C+PCNC G S+ F SGQC+C V G +C++C GYFN+ C C CS L +CD G+C C N C +C + W + T C CNC GSL +C++ TG C C + CS+C G++ + T C P + C+ E G C C N G C++C + D +GC C C+
Sbjct: 42 LASNALITTNATCGEKGPEMYCKLVEHVPGQPVRNPQ----CRICNQNSSNPYQRHPITNA-IDGKNTWWQSPSIKNGVEYHYVTITLDLQQVFQIAYVIVKAANSPRPGNWILERSLDDVEYKPWQYHAVTDTECLTLYNIYPRTGP---------PSYAKDDEVICTSFYSKIHPLENGEIHISLINGRPSADDPS-------PE-LLEFTSARYIRLRFQRIRTLNADLMMFAHKDPREIDPIVTRRYYYSVKDISVGGMCICYGHARACP--LDPATNKSRCECEHNTCGESCDRCCPGFHQKPWRAGTFLTKSECEACNCHGKAEECYYDETVASRNLSLNIHGKYIGGGVCINCTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTGSLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYP--DCQPCNCSGLGS--TNEDPCVGPCSCKENVEGED-CSRCKSGFFNLQEDNQKGCEECFCSGVSNRCQSSYWTYGNIQDMRGWYLTDLSGRIRMAPQLDNPDSPQQISISNS--EARKSLLDGYYWSAPPPYLGNRLPAVGGQLSFTISYDLEEEEDDTEKILQLMIIFEGNDLRI--STAYKE--------VYLEPSEEHIEEVSLKEEAFTIHGTNLPVTRKDFMIVLTNLERVLMQITYNLGMDAIFRLSSVNLESAVPYPTDRRIATDVEVCQCPPGYSGSSCETCWPRHRRVNGTIFGGICEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRCNINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQL-GRGCLPCNCNSFG---SKSFDCEASGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIACDCS--HLGNNCDPKTGQCICPPNTTGEKCSECLP-NTWGHSIVTGCKVCNCSTVGSLASQCNVNTGQCSCHPKFSGMKCSECSRGHWNYPLCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCRPGKFGLDAKNPLGCSSCYCF 1165 HSP 2 Score: 226.098 bits (575), Expect = 4.430e-58 Identity = 156/425 (36.71%), Postives = 206/425 (48.47%), Query Frame = 2
Query: 2654 CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSEN------CDKLTGK-CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKC--ENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSN----ELTK--CDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
C+ C+C + E+CD +TGEC C +T G C C GFYGD S C+ C C L S N D+ G CD C + +C+ C G + + C PC C S C++ SG C C G CE C +GYF N NC+ C C+ + + S C G+C+C NV R CD+C E R C+PCNC GS + C+ +G C C VA + C +C GYF GC C + G C+ + G C+CPPNT G C++C ++W + GCK CNC GS ++ CN +G C+C F G KC C+ G++ +P C C C GT + E K C TGQC CK NV G C CR G F D GC SC+CFGV+ +C
Sbjct: 750 CEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCAC-PLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRCNINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQLGR-GCLPCNCNSFGSKSFDCEA-SGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIACDCSHLGNNCDPKTGQCICPPNTTGEKCSECLPNTWGHSIVTGCKVCNCSTVGSLASQCNVNTGQCSCHPKFSGMKCSECSRGHWNYPLCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCRPGKFGLDAKNPLGCSSCYCFGVTSQC 1171 HSP 3 Score: 222.246 bits (565), Expect = 6.788e-57 Identity = 198/664 (29.82%), Postives = 280/664 (42.17%), Query Frame = 2
Query: 1007 VAKCNCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCN---------RKSNECSYNQTVATLQLSVNKMGRM---EGG-----------------GVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTS----FYRCK-----PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKF-TSFQYLTSENSYIDERFNNPTV---------------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCG---DFRLF--HSVSIEDRRLQRQVFIYIKPTEWKIGKLTP--DKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQ--MTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQE---SCDPVTGECRCSYNTMGTECQLCKH 2761
+C C+ N G +C+ C P F + + C CNCN S +C VA + G EGG G CI C NT G C +C+ + V C C C +G++ S C N G C C + G+ C CS G+ YP C C C + G+ T+ +C C+CKV+V C C+ G F LD+ NP GC CYCFG+T+QCS AK L ++ W T + E+ LP + Q+ T++ F P + +YW P + G + +YG +Y + E + G P VII+ G R+ H + +L R I + EWK P + T+E+F+ L + +LIKA Y + + + I++ E L P IE+C+CPPG++GLSCE+C G+ R+ + G C C C HS+ CD CQNC H+TAG CE CA GYYG + N C+ C CP + + C E+Y C C G +C+RC GY G+P SC+ C+C+ CD VTG C C G +C C+H
Sbjct: 930 TGQCECRPNVQGRHCDECKPETFGLQLGR---GCLPCNCNSFGSKSFDCEASGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIACDCSHLGNNCDPKTGQCI-CPPNTTGEKCSECLPNTWGHSIVTG-----CKVCNCSTVGSLASQCNVN--------TGQCSCHPKFSGMKCSECSRGHWNYP--LCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVH-CDRCRPGKFGLDAKNPLGCSSCYCFGVTSQCSE--AKGL----IRTW--VTLSDEQTILPLVDEALQHTTTKGI----AFQKPEIVAKMDEVRQELHLEPFYWKLPQQFEGKKLMAYGGKLKYAIYFEARDE-TGFATYKPQVIIRGGTPTHARIITRHMAAPLIGQLTRHE-IEMTEKEWKYYGDDPRISRTVTREDFLDILYDIHYILIKATYGNVVRQSRISEISMEVAEPGHVLAGSPPAHLIERCDCPPGYSGLSCETCAPGFYRLRSEPGGRTPGPTLGTCVPCQCNGHSSQCDPETSVCQNCQHHTAGDFCERCALGYYGIVRGLP-NDCQPCACPLISPSNNFSPSCVLEGLEDYRCTACPRGYEGQYCERCAPGYTGSPSSPGGSCQECECDPYGSLPVPCDRVTGLCTCRPGATGRKCDGCEH 1558 HSP 4 Score: 146.362 bits (368), Expect = 5.097e-34 Identity = 163/651 (25.04%), Postives = 268/651 (41.17%), Query Frame = 2
Query: 4289 DSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTA--VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKIN--------ISIQMSSELLNSINK--FYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTD----------INETISGHEQLGISN--ELIKNATAYLETIKN----IYKEMPNITLAAS--VLKDVLKYVDELNQIIKYTQSHIQS----------DQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKEL 6097
D I+ +TR+ F+ L ++ + +K Y + V +I I+ +V E + A +E+C C G C+ G+Y+ S G RT L C CNG+S CD T CQNC + G+ C+ C G+Y + + C+PC CP S NF+ +C +E + +C C GY G+ CE C G+ G+P G C+ C+C+ GS + C+ G+C C PG G KCD C + E C C CT LL + L + +N LP+ + N +S Q + E L + + L E + K+ D ++ + E+ +R + E ++ D +NET+ ++ N EL K L+ +++ KE+ L A+ +LK V K E + + +Q D + L+ +KT A+ ++ N++ + + TKK+ E I +K+ I + + + I +D I + +S +I+DL + + L K + H Q + S+ L L + N NA A AYSNI +D + + + KEL
Sbjct: 1312 DDPRISRTVTREDFLDILYDIHYILIKATYGNVVRQS--RISEISMEVAEPGHVLAGSPPAHLIERCDCPPGYSGLSCETCAPGFYRL-----RSEPGG--RTPGPTLGTCVPCQCNGHSSQCDPETSVCQNCQHHTAGDFCERCALGYYGIVRGLPNDCQPCACPLISPSNNFSPSCV-LEGLEDYRCTACPRGYEGQYCERCAPGYTGSPSSPGGSCQECECDPYGSLPVPCDRVTGLCTCRPGATGRKCDGCEHWHAREGAECVFCGDECTGLLLGDLARLEQMTMNINLTGPLPAPYKILYGLENTTQELKHLLSPQRAPERLIQLAEGNVNTLVMETNELLTRATKVTADGEQTGQDAERTNSRAESLEEFIKGLVQDAEAINEKAVQLNETLGNQDKTAERNLEELQKEIDRMLKELRSKDLQTQKEVAEDELVAAEGLLKRVNKLFGEPRAQNEDMEKDLQQKLAEYKNKLDDAWDLLREATDKTRDANRLSAANQKNMTILETKKEAIEGSKRQIENTLKEGNDILDEANRLLGEINSVIDYVDDIKTKLPPMSEELS---DKIDDLAQEIKDRRLAEKVF----QAESHAAQLNDSSAVLDGILDEAKNISFNATAAFRAYSNI-KDYIDEAEKVAREAKEL 1944 HSP 5 Score: 103.605 bits (257), Expect = 4.672e-21 Identity = 88/275 (32.00%), Postives = 118/275 (42.91%), Query Frame = 2
Query: 3086 CLCSPHALSADCD--LNNGKCKCGSNVINRICDKC-ENLHY--WN-----NRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHA 3838
C+C HA + D N +C+C N CD+C H W +++C CNC + C + VA R+ S L++ G G VC N C NT G C C + + N C+ C+C GS S +C K G C CKT F G CDRC GY+G+P+CQPC+C+ G+ +NE D G C CK+NV G C C+ G F + GC CFC GVS +C +
Sbjct: 283 CICYGHARACPLDPATNKSRCECEHNTCGESCDRCCPGFHQKPWRAGTFLTKSECEACNC---------HGKAEECYYDETVASRNLS--------LNIHG-----KYIGGGVCIN-----CTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTGSLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYPDCQPCNCSGLGS-TNE----DPCVGPCSCKENVEGEDCSRCKSGFFNLQEDNQKGCEECFCSGVSNRCQS 525 HSP 6 Score: 97.8265 bits (242), Expect = 2.706e-19 Identity = 78/268 (29.10%), Postives = 121/268 (45.15%), Query Frame = 2
Query: 4304 NSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSL--NCNEF-GICQ-CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
N +TR FM+ L N+ + +++ + + ++ + + + T VE C C G+ C+ + + N T G + + C C +++ CD +TG+C NC + G C C GFY + + + C+PC CP NF+ C ++ SL L C +C IGY+G CE C +G++G P I G C+ C CN S+ +C+ G C C PG G C+ CA Y V K CQ C
Sbjct: 662 NLPVTRKDFMIVLTNLERVLMQITYNLGMDAIFRLSSVNLESAVPYPTDRRIATDVEVCQCPPGYSGSSCETCWPRHRRV-----NGTIFGGI---------CEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTC-HLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPC 914 HSP 7 Score: 88.5817 bits (218), Expect = 1.989e-16 Identity = 49/142 (34.51%), Postives = 75/142 (52.82%), Query Frame = 2
Query: 4646 CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSC 5068
CDS++G C C G C+LC +G++ + N C+PC+C N + + I + +C+CR G+ C+ C+ +G + G C C+CN GS+S +C G C C PGV G KCD+CA Y ++ C +C
Sbjct: 875 CDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRC-----NINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQL-GRGCLPCNCNSFGSKSFDCEASGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIAC 1010 HSP 8 Score: 69.3218 bits (168), Expect = 1.345e-10 Identity = 64/237 (27.00%), Postives = 88/237 (37.13%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY---GNPKKKI------SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN-----CKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCN 3232
C SG+ C+ C G+Y P + +C C+CN + CDP T C+ C ++T G C+ C G+YG L C+ C C L S S N + C L+D +C C G CE+C GY P+ C++C C P+ +L CD G C C R CD CE+ H +CV C
Sbjct: 1378 CPPGYSGLSCETCAPGFYRLRSEPGGRTPGPTLGTCVPCQCNGHSSQCDPETSVCQNCQHHTAGDFCERCALGYYGIVRGLPNDCQPCAC-PLISPSNN---FSPSCVLEG---------------LEDYRCTACP-----------------------RGYEGQYCERCAPGYTGSPSSPGGSCQECECDPYGSLPVPCDRVTGLCTCRPGATGRKCDGCEHWHAREG-AECVFCG 1571 HSP 9 Score: 61.2326 bits (147), Expect = 3.237e-8 Identity = 44/146 (30.14%), Postives = 64/146 (43.84%), Query Frame = 2
Query: 3218 CVPCNCGRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQV---------CNNEGI-----CVCPPNTIGNLCNKCSEDSWDFNETIG--CKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRC 3604
CVPC C S +CD ET C+ C + A C +C +GY+G+ C PC + C EG+ CP G C +C+ + G C++C C GS C++++G+C C+ G KCD C
Sbjct: 1413 CVPCQCNGHS--SQCDPETSVCQNCQHHTAGDFCERCALGYYGIVRGLPNDCQPCACPLISPSNNFSPSCVLEGLEDYRCTACPRGYEGQYCERCAPGYTGSPSSPGGSCQECECDPYGSLPVPCDRVTGLCTCRPGATGRKCDGC 1556 HSP 10 Score: 59.6918 bits (143), Expect = 1.101e-7 Identity = 60/239 (25.10%), Postives = 103/239 (43.10%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKN---DKNYDQ-CEPCQC-PSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE---FGICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCY 5071
T +C C T G C G++++ + ++G+ T + + CNC+G ++ C ++V G C NC N G C+ C +GF++ NY + C+PC C P+ + ++ C + E + C C+ G+ G +C+ C +G++G P C+ C+C+ GS NE G C C V G C +C + +K C+ C+
Sbjct: 297 ATNKSRCECEHNTCGESCDRCCPGFHQKPW------RAGTFLTKSEC----EACNCHGKAEECYYDETVASRNLSLNIHGKYIGGGVCINCTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTG--SLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYP----DCQPCNCSGLGST----NEDPCVGPCSCKENVEGEDCSRCKSGFFNLQEDNQKGCEECF 515
BLAST of Laminin subunit alpha-2 vs. Ensembl Mouse
Match: Lama2 (laminin, alpha 2 [Source:MGI Symbol;Acc:MGI:99912]) HSP 1 Score: 703.745 bits (1815), Expect = 0.000e+0 Identity = 442/1171 (37.75%), Postives = 625/1171 (53.37%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLM--THKG--YLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQ--KPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFT---SFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSL-IDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAA----------TKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDE--MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQE-----SCDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSC---TSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKC-ENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV-TGCKKCPPCYNGQVCNNE----------GICVCPPNTIGNLCNKCSEDSW--DFNETIGCKKCNCY 3514
L NA IT++ATCG GPE++C+LV+ + Q + NP C +C+ + N +RHPI A D N WWQSPS+ G++Y V +T+DL+QV+QI Y+++K +SPRPGNWILERSLD Y PW + A T C YN++ R P PS DDE+ CT++YS+ L +GEI ISLINGR S + L+ F SARYIR RF + TL ADLM HK ++ + RYYYS+++I +GG CIC HA C ++ + ++C C+HNTCG +C+ CCP F+Q+PW+ CE CNC+ K+ EC Y++TVA+ LS+N G+ GGGVCINC NT GINCE C+ GF+RP V PC PC C G++ E + KPG C CK G+ GVNCD C GY YP +C PC CS GS T+ C PC+CK +V +D C CK+G+FNL N +GCE C+C G++N+C S I D++GW LT +G P+ S Q ++ NS + R + YYW+APPPYLGN + + G + + +L+ + I +I + D R+ S + ++ +Y++P+E I +++ + A T+++F+ L ++++L++ Y +I L+ V D +E C CPPG++G SCE+C +RRVN ++ G CE C C+ H+ CD + GEC NC +T G +C +C G+YGD + C+ C CP + C S +C +C +G C+RC EGY+G P SC+ C+CN+N + SCD ++G C C T G C+LC G++GDA+ K+C+ C C+ GS SE C TG+C+CR N R CD C T GL L + C+PCNC G S+ F SGQC+C V G +C++C GYFN+ C C CS L +CD G+C C N C +C N + T C CNC GSL +C++ TG C C + CS+C G++ + T C P + C+ E G C C N G C++C + D +GC C C+
Sbjct: 42 LASNALITTNATCGEKGPEMYCKLVEHVPGQPVRNPQ----CRICNQNSSNPYQRHPITNA-IDGKNTWWQSPSIKNGVEYHYVTITLDLQQVFQIAYVIVKAANSPRPGNWILERSLDDVEYKPWQYHAVTDTECLTLYNIYPRTGP---------PSYAKDDEVICTSFYSKIHPLENGEIHISLINGRPSADDPS--------PELLEFTSARYIRLRFQRIRTLNADLMMFAHKDPREIDPIVTRRYYYSVKDISVGGMCICYGHARACP--LDPATNKSRCECEHNTCGESCDRCCPGFHQKPWRAGTFLTKSECEACNCHGKAEECYYDETVASRNLSLNIHGKYIGGGVCINCTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTGSLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYP--DCQPCNCSGLGS--TNEDPCVGPCSCKENVEGED-CSRCKSGFFNLQEDNQKGCEECFCSGVSNRCQSSYWTYGNIQDMRGWYLTDLSGRIRMAPQLDNPDSPQQISISNS--EARKSLLDGYYWSAPPPYLGNRLPAVGGQLSFTISYDLEEEEDDTEKILQLMIIFEGNDLRI--STAYKE--------VYLEPSEEHIEEVSLKEEAFTIHGTNLPVTRKDFMIVLTNLERVLMQITYNLGMDAIFRLSSVNLESAVPYPTDRRIATDVEVCQCPPGYSGSSCETCWPRHRRVNGTIFGGICEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRCNINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQL-GRGCLPCNCNSFG---SKSFDCEASGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIACDCS--HLGNNCDPKTGQCICPPNTTGEKCSECLPNTWGHSIVTGCKVCNCSTVGSLASQCNVNTGQCSCHPKFSGMKCSECSRGHWNYPLCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCRPGKFGLDAKNPLGCSSCYCF 1165 HSP 2 Score: 213.386 bits (542), Expect = 1.210e-54 Identity = 153/424 (36.08%), Postives = 204/424 (48.11%), Query Frame = 2
Query: 2654 CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS---CKKCDC------DSLGSTSENCDKLTGKCD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKC--ENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSN----ELTK--CDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
C+ C+C + E+CD +TGEC C +T G C C GFYGD S C+ C C ++ T L CD C + +C+ C G + + C PC C S C++ SG C C G CE C +GYF N NC+ C C+ + + S C G+C+C NV R CD+C E R C+PCNC GS + C+ +G C C VA + C +C GYF GC C + G C+ + G C+CPPNT G C++C ++W + GCK CNC GS ++ CN +G C+C F G KC C+ G++ +P C C C GT + E K C TGQC CK NV G C CR G F D GC SC+CFGV+ +C
Sbjct: 750 CEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRCNINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQLGR-GCLPCNCNSFGSKSFDCEA-SGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIACDCSHLGNNCDPKTGQCICPPNTTGEKCSECLPNTWGHSIVTGCKVCNCSTVGSLASQCNVNTGQCSCHPKFSGMKCSECSRGHWNYPLCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCRPGKFGLDAKNPLGCSSCYCFGVTSQC 1171 HSP 3 Score: 164.081 bits (414), Expect = 1.624e-39 Identity = 180/701 (25.68%), Postives = 279/701 (39.80%), Query Frame = 2
Query: 3086 CLCSPHALSADCD--LNNGKCKCGSNVINRICDKC-ENLHY--WN-----NRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICV-CPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNL--TRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSL--NCNEF-GICQ-CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
C+C HA + D N +C+C N CD+C H W +++C CNC + C + VA R+ S + G + G G+C+ C NT G C C + + N C+ C+C GS S +C K G C CKT F G CDRC GY+G+P+CQPC+C+ G+ +NE D G C CK+NV G C C+ G F + GC CFC GVS +C ++S + Y +++ + + L+ R + P + + NS + + L N+ + + F SY+ D + + + + I + + E + +NL TR FM+ L N+ + +++ + + ++ + + + T VE C C G+ C+ + + N T G + + C C +++ CD +TG+C NC + G C C GFY + + + C+PC CP NF+ C ++ SL L C +C IGY+G CE C +G++G P I G C+ C CN S+ +C+ G C C PG G C+ CA Y V K CQ C
Sbjct: 283 CICYGHARACPLDPATNKSRCECEHNTCGESCDRCCPGFHQKPWRAGTFLTKSECEACNC---------HGKAEECYYDETVASRNLS---LNIHGKYIGG----------------GVCINCTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTGSLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYPDCQPCNCSGLGS-TNE----DPCVGPCSCKENVEGEDCSRCKSGFFNLQEDNQKGCEECFCSGVSNRC---------QSSYWTY------GNIQDMRGWYLTDLSG----RIRMAPQLDNPDSPQQISISNSEARKSLLDGYYWSAPPPYLGNRLPAVGGQLS--FTISYDLEEEEDDTEKILQLMIIFEGNDLRISTAYKEVYLEPSEEHIEEVSLKEEAFTIHGTNLPVTRKDFMIVLTNLERVLMQITYNLGMDAIFRLSSVNLESAVPYPTDRRIATDVEVCQCPPGYSGSSCETCWPRHRRV-----NGTIFGGI---------CEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTC-HLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPC 914 HSP 4 Score: 98.9821 bits (245), Expect = 1.127e-19 Identity = 101/349 (28.94%), Postives = 147/349 (42.12%), Query Frame = 2
Query: 1208 GVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTS----FYRCK-----PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKF-TSFQYLTSENSYIDERFNNPTV---------------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCG---DFRLF--HSVSIEDRRLQRQVFIYIKPTEWKIGKLTP--DKAATKEEFISCLVKVDKLLIKAKY 2155
G CI C NT G C +C+ + V C C C +G++ S C N G C C + G+ C CS G+ YP C C C + G+ T+ +C C+CKV+V C C+ G F LD+ NP GC CYCFG+T+QCS AK L ++ W T + E+ LP + Q+ T++ F P + +YW P + G + +YG +Y + E + G P VII+ G R+ H + +L R I + EWK P + T+E+F+ L + +LIKA Y
Sbjct: 1024 GQCI-CPPNTTGEKCSECLPNTWGHSIVTG-----CKVCNCSTVGSLASQCNVNT--------GQCSCHPKFSGMKCSECSRGHWNYP--LCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVH-CDRCRPGKFGLDAKNPLGCSSCYCFGVTSQCSE--AKGL----IRTW--VTLSDEQTILPLVDEALQHTTTKGI----AFQKPEIVAKMDEVRQELHLEPFYWKLPQQFEGKKLMAYGGKLKYAIYFEARDE-TGFATYKPQVIIRGGTPTHARIITRHMAAPLIGQLTRHE-IEMTEKEWKYYGDDPRISRTVTREDFLDILYDIHYILIKATY 1341 HSP 5 Score: 84.3445 bits (207), Expect = 2.822e-15 Identity = 47/131 (35.88%), Postives = 70/131 (53.44%), Query Frame = 2
Query: 4646 CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
CDS++G C C G C+LC +G++ + N C+PC+C N + + I + +C+CR G+ C+ C+ +G + G C C+CN GS+S +C G C C PGV G KCD+CA Y
Sbjct: 875 CDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRC-----NINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQL-GRGCLPCNCNSFGSKSFDCEASGQCWCQPGVAGKKCDRCAHGY 999 HSP 6 Score: 55.4546 bits (132), Expect = 1.600e-6 Identity = 60/239 (25.10%), Postives = 103/239 (43.10%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKN---DKNYDQ-CEPCQC-PSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE---FGICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCY 5071
T +C C T G C G++++ + ++G+ T + + CNC+G ++ C ++V G C NC N G C+ C +GF++ NY + C+PC C P+ + ++ C + E + C C+ G+ G +C+ C +G++G P C+ C+C+ GS NE G C C V G C +C + +K C+ C+
Sbjct: 297 ATNKSRCECEHNTCGESCDRCCPGFHQKPW------RAGTFLTKSEC----EACNCHGKAEECYYDETVASRNLSLNIHGKYIGGGVCINCTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTG--SLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYP----DCQPCNCSGLGST----NEDPCVGPCSCKENVEGEDCSRCKSGFFNLQEDNQKGCEECF 515
BLAST of Laminin subunit alpha-2 vs. Ensembl Mouse
Match: Lamc1 (laminin, gamma 1 [Source:MGI Symbol;Acc:MGI:99914]) HSP 1 Score: 424.861 bits (1091), Expect = 7.958e-121 Identity = 311/1052 (29.56%), Postives = 486/1052 (46.20%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAK---SDDDNLWWQSPSMAQGLKYQN-VNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSL--DGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNL-KIS-----DLKGWKLTTFTGEKIALPKFTSFQYLTS-ENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR-----LFHSVSIEDRRLQRQVFIYIKPTE--WKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQ-GQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS----CKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS----CKKCDCDSLGSTSE--NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--PNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWN 3205
N + ++ TCGT PE +C + + C +CD + +H F + D WWQS +M G++Y N +N+T+ L + + I Y+ LK S RP ++ + + DG PWI + SC N + + T + +L CT +S L G + S + GR S N + + L +++A IR + T ++ + + + YYY++ + +GG+C CN HA +C ++N CNCKHNT G +CE C P FN RPW+ C C+CN +S EC ++ ++ R G GG C NC++NT+G CE+C + F+R N A C+PC C +G++ + C S G C CK G G CD C G+ C PC C GS C CK +V + C+ CK G+FNL+S+NP+GC PC+CFG ++ C++ ++ IS D GW++ G + +L + Q + +SY Y+ AP +LGN + SYG + +++ + T + + D++++ R + S + +F + T+ W+ A + EF L + + I+ YS + + L ++TL + +E C CP G+ G CE+C GYRR L C C C HS TCD G C +C NTAG HCE C+ GYYGD+ + C+ CPCP + ++ VC C T+G C+ C +GY+G+P C+ C+CN+N + +C+ +TGEC +C YNT G C CK GF+G+ L + CK C C+ G+ + +C+ +TG+C C + R C +C G Y + C C+C G + + C+ +GQC C + G CE+C +F + CK C C +LS C +G+C+C + CD+CE +++N
Sbjct: 58 NVTVVATNTCGTP-PEEYCVQTGVTGVTKS--------CHLCDAGQQHLQHGAAFLTDYNNQADTTWWQSQTMLAGVQYPNSINLTLHLGKAFDITYVRLKFHTS-RPESFAIYKRTREDG----PWIPYQYYSGSCENTYSKANRGFIRTGGDEQQAL------CTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPV------LQEWVTATDIRVTLNRLNTFGDEV-----FNDPKVLKSYYYAISDFAVGGRCKCNGHASEC---VKNEFDKLMCNCKHNTYGVDCEKCLPFFNDRPWRRATAESASECLPCDCNGRSQECYFDP----------ELYRSTGHGGHCTNCRDNTDGAKCERCRENFFRLGNTEA-----CSPCHCSPVGSLSTQCDSYGR---------CSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDPSGSTDECNVETGR-CVCKDNVEGFN-CERCKPGFFNLESSNPKGCTPCFCFGHSSVCTNAVGYSVYDISSTFQIDEDGWRVEQRDGSEASLEWSSDRQDIAVISDSYFPR--------YFIAPVKFLGNQVLSYGQNLSFSFRVDRRDT----RLSAEDLVLEGAGLRVSVPLIAQGNSYPSETTVKYIFRLHEATDYPWR-------PALSPFEFQKLLNNLTSIKIRGT-YSERSAGYLDDVTLQSARPGPGVPAT-WVESCTCPVGYGGQFCETCLPGYRRETPSLGPYSPCVLCTCNGHSETCDPETGVC-DCRDNTAGPHCEKCSDGYYGDSTLGTSSDCQPCPCP----GGSSCAIVPKTKEVVCTHCPTGTAGKRCELCDDGYFGDPLGSNGPVRLCRPCQCNDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKEGFFGNPLAPNPADKCKACACNPYGTVQQQSSCNPVTGQCQCLPHVSGRDCGTCDPGYYNLQSGQGCERCDCHALGSTNGQ--CDIRTGQCECQPGITGQHCERCETNHFGFGPEGCKPCDCHHEGSLSLQCK-EDGRCECREGFVGNRCDQCEENYFYN 1020 HSP 2 Score: 155.992 bits (393), Expect = 5.605e-37 Identity = 167/627 (26.63%), Postives = 258/627 (41.15%), Query Frame = 2
Query: 3281 CKCAQNVARRDCSKC-----DVGYFGLDVTGCKKCPPCY-NG--QVC----------NNEGICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPL--TMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQ---YQNDILDKFII-LEDSN--VINSNLTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIKGIP----CKSCDCNQQGSQSL--NCNEF-GIC-QCSPGVLGIKCDQCAPNY 5038
C C N DC KC D + +C PC NG Q C + G C C NT G C +C E+ + T C C+C GS S C+ G C+CK G KCDRC G++ C+PCSC +G+ +C+ TG+C CK NV G C+ C+ G F + + GC CFCFG S C + + + + I DE +D S L + R+ + + F ++ +K + LS + +++ + VD D + E ++ +E G+R V +I+Q Y ++ K+I L ++ L+ F L N+TS+K++ Y+++ L + + + + G T VE C C G C+ + GY +E +L + +L C CNG+S+ CD TG C +C N G C+ C +G+Y + C+PC CP +C + + E+ C C G +GK CE C G++G+P+ C+ C CN + NCN G C +C G CD+C +
Sbjct: 303 CNCKHNTYGVDCEKCLPFFNDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGHGGHCTNCRDNTDGAKCERCRENFFRLGNTEACSPCHCSPVGSLSTQCDSY-GRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDPSGSTD----ECNVETGRCVCKDNVEGFNCERCKPGFFNLESSNPKGCTPCFCFGHSSVCTNAVGYSVYD--ISSTFQIDEDGWRVEQRDGSEASLEWSSDRQDIAVISDSYFPRYFIAPVKFLGNQVLS-YGQNLSFSFRVDRRDTRLSAE-----DLVLEGA----GLRVSVP----------------------LIAQGNSYPSETTVKYIFRLHEATDYPWRPALSPFEFQKLLNNLTSIKIRGTYSERSAGYLDDVTLQSAR-----PGPGVPATWVESCTCPVGYGGQFCETCLPGYRRETPSLGPYSPC--------VL-----CTCNGHSETCDPETGVC-DCRDNTAGPHCEKCSDGYYGDSTLGTSSDCQPCPCPG-----GSSCAIVPKTKEVVCTHCPTGTAGKRCELCDDGYFGDPLGSNGPVRLCRPCQCNDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKEGF 866 HSP 3 Score: 145.591 bits (366), Expect = 7.015e-34 Identity = 104/339 (30.68%), Postives = 155/339 (45.72%), Query Frame = 2
Query: 2594 GVHCDRCIEGYYGNPKK---KISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDA---LTKSCKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKD--------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP-------NCKKCLCSPHAL---SADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNC-GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQV---CNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC-NCY 3514
G C+ C+ GY C C CN + E+CDP TG C C NT G C+ C G+YGD+ + C+ C C S + C C T ++C+ C G Y D + C PC C ++ CN +G+C C+ N G C++C EG+F P CK C C+P+ + C+ G+C+C +V R C C+ +Y + C C+C GS N +CD+ TG C+C + + C +C+ +FG GCK C + G + C +G C C +GN C++C E+ + GC++C CY
Sbjct: 696 GQFCETCLPGYRRETPSLGPYSPCVLCTCNGHSETCDPETGVCDCRDNTAGPHCEKCSDGYYGDSTLGTSSDCQPCPCPGGSSCAIVPKTKEVVCTHCPTGTAGKRCELCDDG-YFGDPLGSNGPVRLCRPCQCNDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKEGFFGNPLAPNPADKCKACACNPYGTVQQQSSCNPVTGQCQCLPHVSGRDCGTCDPGYYNLQSGQGCERCDCHALGSTNGQCDIRTGQCECQPGITGQHCERCETNHFGFGPEGCKPCDCHHEGSLSLQCKEDGRCECREGFVGNRCDQCEENYFYNRSWPGCQECPACY 1033 HSP 4 Score: 136.346 bits (342), Expect = 4.935e-31 Identity = 116/351 (33.05%), Postives = 153/351 (43.59%), Query Frame = 2
Query: 2711 CRCSYNTMGTECQLCKHGFYGDALT----KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKD-----KKCIPCNCEIQGRISSEKFCNTTSGQC-FCLENVNGDRCEKCVEGYFNYPN--------CKKCLCS----PHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWN----NRTD-CVPCNC---GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV-TGCKKCPPCY-----NGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY---GFPNCQPC 3643
C C G C+ C G+ + + C C C+ SE CD TG CDCR N C+ C+ G Y C PC C G S T C C G RCE C +GYF P C+ C C+ P+A+ +C+ G+C KC N CD+C+ + N N D C C C G C+ TG C+C +V+ RDC CD GY+ L GC++C C+ NGQ G C C P G C +C + + F GCK C+C+ GS S C K G C C+ F G++CD+C E Y+ +P CQ C
Sbjct: 688 CTCPVGYGGQFCETCLPGYRRETPSLGPYSPCVLCTCNG---HSETCDPETGVCDCRDNTAGPHCEKCSDGYYGDSTLGTSSDCQPCPC--PGGSSCAIVPKTKEVVCTHCPTGTAGKRCELCDDGYFGDPLGSNGPVRLCRPCQCNDNIDPNAV-GNCNRLTGECLKCIYNTAGFYCDRCKEGFFGNPLAPNPADKCKACACNPYGTVQQQSSCNPVTGQCQCLPHVSGRDCGTCDPGYYNLQSGQGCERCD-CHALGSTNGQCDIRTGQCECQPGITGQHCERCETNHFGFGPE-GCKPCDCHHEGSLSLQC-KEDGRCECREGFVGNRCDQCEENYFYNRSWPGCQEC 1029 HSP 5 Score: 101.293 bits (251), Expect = 2.411e-20 Identity = 114/480 (23.75%), Postives = 206/480 (42.92%), Query Frame = 2
Query: 4613 KKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINET----ISGHEQLGISNELIKNATAYLETIK----NIYKEMPNIT--------LAASVLKDVLKYVDELNQIIKY--TQSHIQSDQIN-NLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQ----KIFRKMTKQRDNLRKKIQ-KLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQ 5950
K C CN Y V C+ VTG CQ C+ + G C C G+Y N ++ CE C C + Q R +C+C+ G +G+ CE C+ +G +G CK CDC+ +GS SL C E G C+C G +G +CDQC NY + + C +CY LV+ D + E + + L+ + + M ++ ++ + + E+ + + Q++KD + ++++ + + + L++ I ET ++ + +LI+ A+ LE K N+ P T LA K ++ E + I++ T + ++ N L+ L + A EI N++Y + ++ E ++E K +I+ + + + ++ + + I K D LI +++ EDL +++ K+L+ K EQQ
Sbjct: 880 KACACNPYGTVQQQSSCNPVTGQCQ-CLPHVSGRDCGTCDPGYY-NLQSGQGCERCDCHALGSTNGQCDIRTG-----QCECQPGITGQHCERCETNHFGFGPEG--CKPCDCHHEGSLSLQCKEDGRCECREGFVGNRCDQCEENYFYNRSWPGCQECPACYR------LVK-DKVAEHRVKLQ------ELESLIANLGTGDDMVTDQAFE-DRLKEAEREVTDLLREAQEVKDVDQNLMDRLQRVNSSLHSQISRLQNIRNTIEETGILAERARSRVESTEQLIEIASRELEKAKMAAANVSITQPESTGEPNNMTLLAEEARKLAERHKQEADDIVRVAKTANETSAEAYNLLLRTLAGENQTALEIEELNRKYEQAKNI-SQDLEKQAARVHEEAKRAGDKAVEIYASVAQLTPVDSEALENEANKIKKEAADLDRLIDQKLKDYEDLREDMRGKEHEVKNLL----EKGKAEQQ 1331 HSP 6 Score: 99.7525 bits (247), Expect = 6.507e-20 Identity = 97/305 (31.80%), Postives = 127/305 (41.64%), Query Frame = 2
Query: 3005 CFCLENVNGDRCEKCVEGYFN-------YPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNN----RTDCVPCNCGRGS---LNPKCDMETGNCKCAQNVARRDCSKCDVGYFG--LDVTG-CKKCPPCY-NGQV-------CNN-EGICV-CPPNTIGNLCNKCSE----DSWDFNETIGCKKCNCYINGSYS--NICNKISGICACKTAFFGSKCDRCNEGYYGFPN---CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
C C G CE C+ GY Y C C C+ H S CD G C C N C+KC + +Y ++ +DC PC C GS + PK E C A + C CD GYFG L G + C PC N + CN G C+ C NT G C++C E + N CK C C G+ + CN ++G C C G C C+ GYY + C+ C C G+ + + CD TGQC+C+ + G C+ C F G GC C C
Sbjct: 688 CTCPVGYGGQFCETCLPGYRRETPSLGPYSPCVLCTCNGH--SETCDPETGVCDCRDNTAGPHCEKCSDGYYGDSTLGTSSDCQPCPCPGGSSCAIVPKT-KEVVCTHCPTGTAGKRCELCDDGYFGDPLGSNGPVRLCRPCQCNDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKEGFFGNPLAPNPADKCKACACNPYGTVQQQSSCNPVTGQCQCLPHVSGRDCGTCDPGYYNLQSGQGCERCDCHALGSTNGQ---CDIRTGQCECQPGITGQHCERCETNHF---GFGPEGCKPCDC 983 HSP 7 Score: 92.0485 bits (227), Expect = 1.199e-17 Identity = 75/255 (29.41%), Postives = 116/255 (45.49%), Query Frame = 2
Query: 2726 NTMGTEC----QLCKHGFYGDALTKSCKKCDCDSLGS--TSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK-----------KCIPCNCEIQGR---ISSEKFCNTT-SGQCF-CLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY---NGQVCNN 3403
NT G E ++ K +Y + +C C+ S DKL C+C+ N C+ C + D+ +C+PC+C + + E + +T G C C +N +G +CE+C E +F N C C CSP +LS CD + G+C C V+ CD+C+ + C PC+C +C++ETG C C NV +C +C G+F L+ + K C PC+ + VC N
Sbjct: 255 NTFGDEVFNDPKVLKSYYYAISDFAVGGRCKCNGHASECVKNEFDKLM--CNCKHNTYGVDCEKCLP--FFNDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGHGGHCTNCRDNTDGAKCERCRENFFRLGNTEACSPCHCSPVGSLSTQCD-SYGRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDPSGSTDECNVETGRCVCKDNVEGFNCERCKPGFFNLESSNPKGCTPCFCFGHSSVCTN 504 HSP 8 Score: 73.559 bits (179), Expect = 5.192e-12 Identity = 54/165 (32.73%), Postives = 73/165 (44.24%), Query Frame = 2
Query: 4619 CNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCY 5071
C+CNG S+ C G C NC N +G KC+ CRE F++ N + C PC C S + + C +C C+ G G C+ CQ GF+ G C+ C C+ GS CN E G C C V G C++C P + K C C+
Sbjct: 340 CDCNGRSQECYFDPELYRSTGHGGHCTNCRDNTDGAKCERCRENFFRL-GNTEACSPCHC-SPVGSLSTQCDSYG-----RCSCKPGVMGDKCDRCQPGFHSLTEAG--CRPCSCDPSGSTD-ECNVETGRCVCKDNVEGFNCERCKPGFFNLESSNPKGCTPCF 494 HSP 9 Score: 63.1586 bits (152), Expect = 8.704e-9 Identity = 60/200 (30.00%), Postives = 84/200 (42.00%), Query Frame = 2
Query: 4517 NPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGD---CQNCMGNREGNKCQLCREGFYKNDKNY--------DQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFY--GNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY-VVEKKTCQSC 5068
N ++ + E FN P KS + + +C CNG++ C D C NC N G C+ C F ND+ + +C PC C S F R CR G CE C++ F+ GN C C C+ GS S C+ +G C C PGV+G KCD+C P + + + C+ C
Sbjct: 252 NRLNTFGDEVFNDPKVLKSYYYAISDFAVGG--RCKCNGHASECVKNEFDKLMC-NCKHNTYGVDCEKCLPFF--NDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGHGGHCTNCRDNTDGAKCERCRENFFRLGNTEA---CSPCHCSPVGSLSTQCDSYGRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPC 443
BLAST of Laminin subunit alpha-2 vs. Ensembl Mouse
Match: Lamc3 (laminin gamma 3 [Source:MGI Symbol;Acc:MGI:1344394]) HSP 1 Score: 397.897 bits (1021), Expect = 1.694e-112 Identity = 323/1066 (30.30%), Postives = 476/1066 (44.65%), Query Frame = 2
Query: 191 MENA----KITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFA---KSDDDNLWWQSPSMAQGLKYQ-NVNVTIDLKQVYQIVYILLKMGDSPRPGNW-ILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPP---CNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSA--KNLKISDLK----GWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSV------SIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLI-KAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRR-VNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP----KKKISCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTK----SCKKCDCDSLGSTSE-NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCE-NLHYWNNRTDCVPC 3229
ENA + +S TCG PE FC V P + + C CD+ + +RH + S DD+ WWQSPSMA G++Y +VN+T+ L + Y+I Y+ LK S RP ++ I +R+ + P+ +++ SC + R P+ + +A FCT+ +S LN G + S + GR S N E + L ++++ I + T D+ + + + YYY++ + +GG+C CN HA +C+ N C C+HNT G +CE C P F RPW C CNC+ S EC++++ ++ R G GG C C+++T G +CE+C K +YR W + PC PC CH G++ N G C CK G CD C G+ G C PC C++ GS+ T C P C CK +V LC C+ G FNL NP GC C+C+G + CS + ++ SD + GW++ + K L Q+ S +AP +LG+ SYG V + LQ GS P + ++ L S+ S +D R R+V + E +P + +S L + + +S Q + + +T + + LA +E C CP G+ G CE C GY+R + + C C C H TCD G C C H+T G CE C G+YG+A S + C+ CPCP + + S + VC C G C+ C +G++G+P C+RC+C+ N + +CDP +G C RC YNT G C+ C+ GFYG A+ C C CD GS SE C+ +TG+C C R C C+ G Y + C C C G + E C+ +GQC C V G C++C G+F + C+ C CSP A S+ C N C C + CD+C+ N + T C C
Sbjct: 48 FENAAFGRRAEASHTCGRP-PEDFCPHVGA--------PGAGLQCQRCDDADPGRRHDASYLTDFHSPDDSTWWQSPSMAFGVQYPTSVNLTLSLGKAYEITYVRLKFHTS-RPESFAIYKRTYASGPWEPYQYYSA---SCQKTYGR--PEGHYLRPGEDERVA----FCTSEFSDISPLNGGNVAFSTLEGRPSAYNFEESPV------LQEWVTSTDILISLDRLNTFGDDI-----FKDPRVLQSYYYAVSDFSVGGRCKCNGHASECEP---NAAGQLACRCQHNTTGVDCERCLPFFQDRPWARGTAEDANECLPCNCSGHSEECTFDR----------ELYRSTGHGGHCQRCRDHTTGPHCERCEKNYYR-W----SPKTPCQPCDCHPAGSLSLQCDNS--------GVCPCKPTVTGWKCDRCLPGFHSLSEGGCRPCACNVAGSLGT----CDPRSGNCPCKENVEGS-LCDRCRPGTFNLQPHNPVGCSSCFCYGHSKVCSPAAGFQEHHIRSDFRHGAGGWQIRSMGVSKRPL------QWSQSGLLLGLR-----GGEELSAPKKFLGDQRLSYGQ----PVILTLQVPPGGS---PPPIQLRLEGAGLALSLRPSSLPSPQDTRQPRRVQLQFLLQETSEEAESPLPTFHFQRLLSNLTALSIWTSGQGPGHSGQVLLCEVQLTSAWPQRELA-PPASWVETCLCPQGYTGQFCEFCALGYKREIPHGGPYANCIPCTCNQH-GTCDPNTGICL-CGHHTEGPSCERCMPGFYGNAFSGRADDCQPCPCP----GQSACATIPESGDVVCTHCPPGQRGRRCESCEDGFFGDPLGLSGAPQPCRRCQCSGNVDLNAVGNCDPHSGHCLRCLYNTTGAHCEHCREGFYGSAVATRPVDKCAPCSCDLRGSVSEKTCNPVTGQCVCLPYVSGRDCSRCSPGFYDLQSGRGCQSCKCHPLGSL--ENKCHPKTGQCPCRPGVTGQACDRCQLGFFGFSIKGCRDCRCSPLGAASSQCH-ENSTCVCRPGFVGYKCDRCQDNFFLADGDTGCQEC 1024 HSP 2 Score: 140.584 bits (353), Expect = 2.248e-32 Identity = 166/692 (23.99%), Postives = 263/692 (38.01%), Query Frame = 2
Query: 3083 KCLCSPHALSADCDLNNG---KCKCGSNVINRICDKCENLHYWNNRT----------DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVM------KLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETT----QAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI----KGIPCKSCDCNQQGSQSLNCNEFGICQCSPG--------VLGIKCDQCAPNY 5038
+C C+ HA ++C+ N C+C N C++C L ++ +R +C+PCNC + C + + R G+ G C++C +T G C +C ++ + ++ C+ C+C+ GS S C+ SG+C CK G KCDRC G++ C+PC+C G+ L CD +G C CK+NV G+ C CR GTF GC SCFC+G S+ C E+HI ++ G I R W + K LS + + + + + V + P ++ +E +R S + ++ + +F++ E S S L F L N+T+L + +T P +++ +V T+ + + + VE C+C G C+ GY +E +P+ N C CN + CD TG C C + EG C+ C GFY N D C+PC CP C I S ++ C C G G+ CE+C+ GF+G+P+ PC+ C C S +++ N G C G G C+ C +
Sbjct: 279 RCKCNGHA--SECEPNAAGQLACRCQHNTTGVDCERC--LPFFQDRPWARGTAEDANECLPCNC---------SGHSEECTFDRELYR------STGHGG----HCQRCRD-----------------HTTGPHCERCEKNYYRWSPKTPCQPCDCHPAGSLSLQCDN-SGVCPCKPTVTGWKCDRCLPGFHSLSEGGCRPCACNVAGS----LGTCDPRSGNCPCKENVEGSLCDRCRPGTFNLQPHNPVGCSSCFCYGHSKVCSPAAG-------FQEHHI---RSDFRHGAGGWQIRSMGVSKRPLQWSQSGLLLGLRGGEELSAPKKFLGDQRLS-YGQPVILTLQVPPGGS------PPPIQLRLEGAGLALSLRPSSLPSPQD-------------------TRQPRRVQLQFLLQETSEEAESPLPTFHFQRLLSNLTALSI--WTSGQGPGHSG-QVLLCEVQLTSAWPQRELAPPASWVETCLCPQGYTGQFCEFCALGYKRE---IPHGGP----------YANCIPCTCNQHG-TCDPNTGICL-CGHHTEGPSCERCMPGFYGNAFSGRADDCQPCPCPG-----QSACATIPESGDVVCTHCPPGQRGRRCESCEDGFFGDPLGLSGAPQPCRRCQC----SGNVDLNAVGNCDPHSGHCLRCLYNTTGAHCEHCREGF 862 HSP 3 Score: 120.553 bits (301), Expect = 3.360e-26 Identity = 104/372 (27.96%), Postives = 155/372 (41.67%), Query Frame = 2
Query: 2858 CDCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN------YPNCKKCLCSPHALSADCDLNNGKC--KCGSNVINRICDKCEN------LHYWNNRTDCVPCNC-GRGSLNP--KCDMETGNC-KCAQNVARRDCSKCDVGYFGLDVT--GCKKCPPC-------YNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC-FCFGVSEKCHAILNHRIV 3859
C C + + C+ C G Y ++ CIPC C G C+ +G C C + G CE+C+ G++ +C+ C C + A + C R C+ CE+ L C C C G LN CD +G+C +C N C C G++G V KC PC + + CN G CVC P G C++CS +D GC+ C C+ GS N C+ +G C C+ G CDRC G++GF C+ C C+ G S++ + C C+ +G KC C+ F +DG + GC C C+ + ++ A L R++
Sbjct: 685 CLCPQGYTGQFCEFCALG-YKREIPHGGPYANCIPCTCNQHGT------CDPNTGICLCGHHTEGPSCERCMPGFYGNAFSGRADDCQPCPCPGQSACATIPESGDVVCTHCPPGQRGRRCESCEDGFFGDPLGLSGAPQPCRRCQCSGNVDLNAVGNCDPHSGHCLRCLYNTTGAHCEHCREGFYGSAVATRPVDKCAPCSCDLRGSVSEKTCNPVTGQCVCLPYVSGRDCSRCSPGFYDLQSGRGCQSCKCHPLGSLENKCHPKTGQCPCRPGVTGQACDRCQLGFFGFSIKGCRDCRCSPLGAASSQCHE----NSTCVCRPGFVGYKCDRCQDNFFLADG--DTGCQECPTCYALVKEEAAKLKARLM 1043 HSP 4 Score: 112.464 bits (280), Expect = 8.225e-24 Identity = 99/347 (28.53%), Postives = 140/347 (40.35%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGY-----YGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALT---KSCKKCDC--------------------------------------DSLGST-----------SENCD-KLTGKCD--------CRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV-TGCKKCPPCY 3382
C +G C+ C GY +G P +C C CN + +CDP TG C C ++T G C+ C GFYG+A + C+ C C D LG + S N D G CD C N C+ C G Y KC PC+C+++G + SEK CN +GQC CL V+G C +C G+++ + C+ C C P +L C G+C C V + CD+C+ + + C C C G+ + +C E C C C +C +F D TGC++CP CY
Sbjct: 687 CPQGYTGQFCEFCALGYKREIPHGGPY--ANCIPCTCNQHG-TCDPNTGICLCGHHTEGPSCERCMPGFYGNAFSGRADDCQPCPCPGQSACATIPESGDVVCTHCPPGQRGRRCESCEDGFFGDPLGLSGAPQPCRRCQCSGNVDLNAVGNCDPHSGHCLRCLYNTTGAHCEHCREGFYGSAVATRPVDKCAPCSCDLRGSV-SEKTCNPVTGQCVCLPYVSGRDCSRCSPGFYDLQSGRGCQSCKCHPLGSLENKCHPKTGQCPCRPGVTGQACDRCQLGFFGFSIKGCRDCRCSPLGAASSQCH-ENSTCVCRPGFVGYKCDRCQDNFFLADGDTGCQECPTCY 1028 HSP 5 Score: 93.9745 bits (232), Expect = 3.666e-18 Identity = 84/346 (24.28%), Postives = 141/346 (40.75%), Query Frame = 2
Query: 4613 KKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKN---DKNYDQCEPC------------------QCPSAHYNFAQNCTRIE--------------------SSLELKCQ-------CRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYSGCTKD-------LLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRST---FTDINETISGHEQLGISNELIKNATA 5446
++C C+G + CD +G C C+ N G C+ CREGFY + + D+C PC QC Y ++C+R SLE KC CR G +G++C+ CQ GF+G IKG C+ C C+ G+ S C+E C C PG +G KCD+C N+ + + C +CY+ ++ L++ L + W LD+ +Q + L + + L+ F Q ++D K + +++ L+ + + T ++ ET+ E+ E+++ A+A
Sbjct: 820 RRCQCSGNVDLNAVGNCDPHSGHCLRCLYNTTGAHCEHCREGFYGSAVATRPVDKCAPCSCDLRGSVSEKTCNPVTGQCVCLPYVSGRDCSRCSPGFYDLQSGRGCQSCKCHPLGSLENKCHPKTGQCPCRPGVTGQACDRCQLGFFGFSIKG--CRDCRCSPLGAASSQCHENSTCVCRPGFVGYKCDRCQDNFFLADGDTGCQECPTCYALVKEEAAKLKARLMLMEGWLQRSDCGSPW----GPLDI--------LQGEAPLGDVYQGHHLLQETRGTFLQQMVGLEDSVKATWEQLQVLRGHVHCAQAGAQKTCIQLAELEETLQSSEE-----EVLRAASA 1146 HSP 6 Score: 79.7221 bits (195), Expect = 6.803e-14 Identity = 73/269 (27.14%), Postives = 105/269 (39.03%), Query Frame = 2
Query: 2609 RCIEGYYGNPKKKISCKRCKCNNNQESCDP-VTGE--CRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGR---ISSEKFCNTT-SGQC-FCLENVNGDRCEKCVEGYFNYPN---CKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGL---DVTGCKKC 3370
R ++ YY RCKCN + C+P G+ CRC +NT G +C+ C F + T+E+ ++ C+PCNC E + +T G C C ++ G CE+C + Y+ + C+ C C P +LS CD N+G C C V CD+C + + C PC C CD +GNC C +NV C +C G F L + GC C
Sbjct: 262 RVLQSYYYAVSDFSVGGRCKCNGHASECEPNAAGQLACRCQHNTTGVDCERCLPFFQDRPWARG-----------TAEDANE-----------------------------CLPCNCSGHSEECTFDRELYRSTGHGGHCQRCRDHTTGPHCERCEKNYYRWSPKTPCQPCDCHPAGSLSLQCD-NSGVCPCKPTVTGWKCDRCLPGFHSLSEGGCRPCACNVAGSLGTCDPRSGNCPCKENVEGSLCDRCRPGTFNLQPHNPVGCSSC 489 HSP 7 Score: 53.9138 bits (128), Expect = 5.594e-6 Identity = 64/267 (23.97%), Postives = 108/267 (40.45%), Query Frame = 2
Query: 929 NIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLF--NQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPC-YCFGITNQCSSLSAKNLKISDLKGW 1720
N+++ C+ H+ C + C +NT G++CE C F + +PV C C+C+ L+ SV++ G C+ C G +C +C GFY + C C+CH LG++ E+ K G C C+ G G CD C +G+ + C C CS G+ + + C C+ C C++ +F D GC+ C C+ + + ++ L + ++GW
Sbjct: 827 NVDLNAVGNCDPHSGHCLR------------CLYNTTGAHCEHCREGFYGSAVATRPVDKCAPCSCD--------------LRGSVSEKTCNPVTGQCV-CLPYVSGRDCSRCSPGFYDLQSGRG-----CQSCKCHPLGSL-------ENKCHPKTGQCPCRPGVTGQACDRCQLGFFGFSIKGCRDCRCSPLGAASSQCHE-NSTCVCRPGFVGYK-CDRCQDNFFLADGDT--GCQECPTCYALVKEEAAKLKARLML--MEGW 1048
BLAST of Laminin subunit alpha-2 vs. UniProt/SwissProt
Match: sp|P25391|LAMA1_HUMAN (Laminin subunit alpha-1 OS=Homo sapiens OX=9606 GN=LAMA1 PE=1 SV=2) HSP 1 Score: 754.592 bits (1947), Expect = 0.000e+0 Identity = 448/1182 (37.90%), Postives = 632/1182 (53.47%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT--HK--GYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNG--EDIGDQK-PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALP----KFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQ-STLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQ--RQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITLSRGEVH-LALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK--KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC------YNGQVCNNE----------GICVCPPNTIGNLCNKCSEDSWDF--NETIGCKKCNCYINGSYSNICNKI 3550
L NA I+++ATCG GPE+FC+LV+ + + + NP C +CD + N +RHPI A D N WWQSPS+ G +Y V +T+DL+QV+Q+ Y+++K ++PRPGNWILERSLDG + PW ++A + C YN+ R+ P P+ ADDE+ CT+YYS+ L GEI SLINGR S + LS K L+ F SARYIR R + TL ADLMT H+ L+ + RYYYS+++I +GG CIC HA C + +C C+HNTCG +C CCP ++Q+PW+P V CE CNC+ K+ +C Y+++VA + S+N G+ GGGVCINC +NT GINCE CI G+YRP V E PC PC C +G++ S C+ + D+ + K PG C CK+GY G CD C +GY+ YP C+ C C+ GS S C PC CK +V C CK G++NL NP GC C+CFG+++ CSSLS +++ + GW +T + +P ++ N+ + +R YYW AP YLGN +T++G +Y V ++ T+ +L+ DVIIK L S E LQ + ++ + +++ ++ L V LLI+A Y S + ++ L +++L + + L +E C CP G+ G SCESC SGY RV+ +L+ G C+ C C+ H+ C+ ++G C C HNT G HCE C G+YG+ C+ C CP + C + VC C SG C+RC +GYYGNP SC C C+ N + CD VTGEC +C NT G C+ C GFYGDA+T K+C+ C+C GS S C TG CDC+ N +QCD C G Y D C PCNC + G +S C T GQC C+ V G RC++C G++ Y + C C C PH + CD G+C C + C++CE+ H+ ++ C CNC GS + +CD+ TG+C+C R C +C +GY C PC +G CN E G C C N G CN+C E ++ + +GC C C S++C+++
Sbjct: 29 LASNAHISTNATCGEKGPEMFCKLVEHVPGRPVRNPQ----CRICDGNSANPRERHPISHAI-DGTNNWWQSPSIQNGREYHWVTITLDLRQVFQVAYVIIKAANAPRPGNWILERSLDGTTFSPWQYYAVSDSECLSRYNITPRRGP---------PTYRADDEVICTSYYSRLVPLEHGEIHTSLINGRPSA---DDLSPK-----LLEFTSARYIRLRLQRIRTLNADLMTLSHREPKELDPIVTRRYYYSIKDISVGGMCICYGHASSCP--WDETTKKLQCQCEHNTCGESCNRCCPGYHQQPWRPGTVSSGNTCEACNCHNKAKDCYYDESVAKQKKSLNTAGQFRGGGVCINCLQNTMGINCETCIDGYYRPHKVSPYEDEPCRPCNCDPVGSLSSVCIKDDLHSDLHNGKQPGQCPCKEGYTGEKCDRCQLGYKDYPT--CVSCGCNPVGSA--SDEPCTGPCVCKENVEG-KACDRCKPGFYNLKEKNPRGCSECFCFGVSDVCSSLSWPVGQVNSMSGWLVTDLISPR-KIPSQQDALGGRHQVSINNTAVMQRL--APKYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETVDSNLMSHADVIIKGNGLTL--STQAEGLSLQPYEEYLNVVRLVPENFQDFHSKRQIDRDQLMTVLANVTHLLIRANYNSAKMALYRLESVSLDIASSNAIDLVVAADVEHCECPQGYTGTSCESCLSGYYRVDGILFGGICQPCECHGHAAECN-VHGVCIACAHNTTGVHCEQCLPGFYGEPSRGTPGDCQPCACPLTIASNNFSPTCHLNDGDEVVCDWCAPGYSGAWCERCADGYYGNPTVPGESCVPCDCSGNVDPSEAGHCDSVTGECLKCLGNTDGAHCERCADGFYGDAVTAKNCRACECHVKGSHSAVCHLETGLCDCKPNVTGQQCDQCLHGYYGLDSGHGCRPCNCSVAGSVSDG--C-TDEGQCHCVPGVAGKRCDRCAHGFYAYQDGSCTPCDC-PHTQNT-CDPETGECVCPPHTQGVKCEECEDGHWGYDAEVGCQACNCSLVGSTHHRCDVVTGHCQCKSKFGGRACDQCSLGYRDF-----PDCVPCDCDLRGTSGDACNLEQGLCGCVEETGACPCKENVFGPQCNECREGTFALRADNPLGCSPCFC---SGLSHLCSEL 1162 HSP 2 Score: 397.512 bits (1020), Expect = 2.907e-109 Identity = 410/1680 (24.40%), Postives = 683/1680 (40.65%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPKKKIS--CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCD-SLGST--SENCDKLTGK---CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCS-PHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNE------LTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYE----YHIFDEKN--SLESGKDYST--IHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDN------------KCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIK-IKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY-KNDKNYDQCEPCQCP-SAHYNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKINISIQMSSELL--NSINKFYKLK-----NEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKV---LEDLRSTFTDINETISGHEQL--------GISNELIKNATAYLET--IKNIYKEMPNITLAASVLKDVLKYVDELNQ-------IIKYTQSHIQSDQINNLKILKNKTIMADE-----------INTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVR-EIEDLMKNLSSNHLV----------SKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFL----HNAEQAKEAYSNITRDILAVDYAINQTKKEL--------DFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDL-----VNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLP---TSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGK-WHMVIRSISDGRTEQVETSSI--TNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRN 7216
+C +G C+ C+ GYY C+ C+C+ + C+ V G C C++NT G C+ C GFYG+ C+ C C ++ S S C G CD C + C+ C G Y + + C+PC+C S C++ +G+C CL N +G CE+C +G++ NC+ C C + SA C L G C C NV + CD+C + +Y ++ C PCNC GS++ C E G C C VA + C +C G++ C C + C+ E G CVCPP+T G C +C + W ++ +GC+ CNC + GS + C+ ++G C CK+ F G CD+C+ GY FP+C PC C GT + L C + TG C CK+NV G +C CR+GTF GC CFC G+S C + ++ ++ + + N G Y L+A R+ P Y + + F L + + +V+ LD K GR I +Q ++M++ G+R E + +Y N + +K + ED FM L ++ + +K Y + I I + + E + + +E C+C T G CQ+ GY++ LP +GS R ++ C+CN +S CD TG C NC N G+ C +C G+Y K + C C CP S +F+ C +E + +C C +GY GK CE C +YGNP G C+ CDCN GS +C+ G C C G G++CD+C P +++ + C SC C LL +D + + V++ +P + N N + + LL N K+K E N + +M ++ E++ + +E L+ + T+I E + ++ L + +N T+ LE I++ + N TL +D+L + E Q ++K SH+ S N LK + A+ +N N +E+ + ++E L LI + + + Q D ++ ++ L+ QD+ L S +R I+DL+ ++S + V ++ + D L +E N S N +N + E A++A+ +T L + ++ K + + N + + + L+E + + EN I ++ + L + IP G D + L+ + + ++Q +LN + R +L++ D+ L V + N + D K +K L E L + +L+ A + I+ V R + Y P ++N +L +K Q ++ L Y S + A + R + F +D G+ +E ++N + + G + ++ +S + +TS T N+L + T +FVGG ++ +S ++ +KGCL L GL + E +CR
Sbjct: 710 ECPQGYTGTSCESCLSGYYRVDGILFGGICQPCECHGHAAECN-VHGVCIACAHNTTGVHCEQCLPGFYGEPSRGTPGDCQPCACPLTIASNNFSPTCHLNDGDEVVCDWCAPGYSGAWCERCADGYYGNPTVPGESCVPCDCSGNVDPSEAGHCDSVTGECLKCLGNTDGAHCERCADGFYGDAVTAKNCRACECHVKGSHSAVCHLETGLCDCKPNVTGQQCDQCLHGYYGLDSGHGCRPCNCSVAGSVSDGCTDE-GQCHCVPGVAGKRCDRCAHGFYAYQDGSCTPCDCPHTQNTCDPETGECVCPPHTQGVKCEECEDGHWGYDAEVGCQACNCSLVGSTHHRCDVVTGHCQCKSKFGGRACDQCSLGYRDFPDCVPCDCDLRGTSGDACNLEQGLCGCVEETGACPCKENVFGPQCNECREGTFALRADNPLGCSPCFCSGLSHLCSELEDYVRTPVTLGSDQPLLRVVSQSNLRGTTEGVYYQAPDFLLDAATVRQH----IRAEPFYWRLPQQFQGDQLMAYGGKLKYSVAFYSLDGVGTSNFEPQVLIKGGR-IRKQV-IYMDAPAPENGVR-----------------QEQEVAMRENFWKYFNSVSEKPVTRED------------FMSVLSDIEYILIKASYGQGLQQSRISDISMEVGRKAEKLHPEEEVASLLENCVCPPGTVGFSCQDCAPGYHRG--KLP----AGSDRGPRPLVAPCVPCSCNNHSDTCDPNTGKCLNCGDNTAGDHCDVCTSGYYGKVTGSASDCALCACPHSPPASFSPTCV-LEGDHDFRCDACLLGYEGKHCERCSSSYYGNPQTPGGSCQKCDCNPHGSVHGDCDRTSGQCVCRLGASGLRCDECEPRHILMETDCVSCDDECVGVLLNDLDEIGDAVLSLNLTGIIPVPYGILSNLENTTKYLQESLLKENMQKDLGKIKLEGVAEETDNLQKKLTRMLASTQKVNRATERIFKESQDLAIAIERLQMSITEIMEKTTLNQTLDEDFLLPNSTLQNMQQNGTSLLEIMQIRDFTQLHQNATLELKAAEDLLSQIQENYQKPLEELEVLKEAASHVLSKHNNELKAAEALVREAEAKMQESNHLLLMVNANLREFSDKKLHVQEEQNLTSELIVQG----RGLIDAAAAQTDAVQDALEHLE----DHQDKLLLWSAKIRHHIDDLVMHMSQRNAVDLVYRAEDHAAEFQRLADVLYSGLENIRNVSLNATSAAYVHYNIQSLIEESEELARDAHRTVTETSLLSESLVSNGKAAVQRSSRFLKEGNNLSRKLPGIALELSELRNKTNRFQENAVEITRQTNESLLILR------------------AIPKGIRDKGAKTKELATSASQSAVSTLRDVAGLSQELLNTSASLSRVNTTLRETHQLLQ--DSTMATLLAGRKVKDVEIQANLLFDRLKPLKMLEENLSRNLSEIKLLISQARKQAASIKVAVSADRDCIRAYQPQISSTNYNTLTLNVKTQEPDN----LLFYLGSSTASDFLAVEMRR-GRVAFLWDLGSGSTRLEFPDFPIDDNRWHSIHVARFGNIGSLSVKEMSSNQKSPTKTSKSPGTANVLD---VNNSTLMFVGGL-GGQIKKSPAVK------------VTHFKGCLGEAFLNGKSIGLWNYIEREGKCRG 2296 HSP 3 Score: 229.565 bits (584), Expect = 2.257e-58 Identity = 192/690 (27.83%), Postives = 286/690 (41.45%), Query Frame = 2
Query: 914 YYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGS------VRTSFYRC---KPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFT-GEKIALPKFTSFQYL--TSENSYI---DERFNNPTV--------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR---LFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRG----EVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQ----------CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR-LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKL 2848
YY L + C C+ D + G +C+C G C+ C F C C+C N C G C+ C +T+G+ CE+C G W A C C C S V + D G C CK + G CD CS+GY +P +C+PC C + G+ + C C CK +V C C+ G F L + NP GC PC+C G+++ CS +L+ + T T G L + S L T+E Y D + TV +YW P + G+ + +YG +Y V + S + P V+IK G R ++ + ++++ + ++ WK +K T+E+F+S L ++ +LIKA Y + + +I++ G ++H + +E C CPPG G SC+ C GY R L G C C+C NHS+TCD G+C NC NTAG HC+ C SGYYG + + + C C CP + C ++ C CL G HC+RC YYGNP+ SC++C CN + CD +G+C C G C C+ + C CD + +G + D++
Sbjct: 939 YYGLDSGHGCRPCNCSVAGSVSDGCTDEG----QCHCVPGVAGKRCDRCAHGFYAYQ---DGSCTPCDCPHTQNTCDPET------------------GECV-CPPHTQGVKCEECEDGH---WGYDAE--VGCQACNC-------SLVGSTHHRCDVVTGHCQCKSKFGGRACDQCSLGYRDFP--DCVPCDCDLRGTSGDACNLEQGLCGCVEETGACPCKENVFGPQ-CNECREGTFALRADNPLGCSPCFCSGLSHLCS----------ELEDYVRTPVTLGSDQPLLRVVSQSNLRGTTEGVYYQAPDFLLDAATVRQHIRAEPFYWRLPQQFQGDQLMAYGGKLKYSVAFYSLDGVGTSNFE-PQVLIKGGRIRKQVIYMDAPAPENGVRQEQEVAMRENFWKYFNSVSEKPVTREDFMSVLSDIEYILIKASYGQGLQQSRISDISMEVGRKAEKLHPEEEVASLLENCVCPPGTVGFSCQDCAPGYHR--GKLPAGSDRGPRPLVAPCVPCSCNNHSDTCDPNTGKCLNCGDNTAGDHCDVCTSGYYGKV-TGSASDCALCACPHSPPASFSPTCVLEGDHDFRCDACLLGYEGKHCERCSSSYYGNPQTPGGSCQKCDCNPHGSVHGDCDRTSGQCVCRLGASGLRCDECEPRHI--LMETDCVSCDDECVGVLLNDLDEI 1571 HSP 4 Score: 168.318 bits (425), Expect = 8.769e-40 Identity = 191/712 (26.83%), Postives = 285/712 (40.03%), Query Frame = 2
Query: 3086 CLCSPHALSADCDLNNGK--CKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFN-----ETIGCKKCNCYINGSYSNIC-----------NKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI---------LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTA-VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILIN--LKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY--KNDKNYDQCEPCQCPS--AHYNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDC--NQQGSQSLNCNEF-GIC-QCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
C+C HA S D K C+C N C++C + W T C CNC + +C ++VA++ S G F G VC N C NT+G C C + + + E C+ CNC GS S++C K G C CK + G KCDRC GY +P C C C G+ S+E TG C CK+NV G C C+ G + GC CFCFGVS+ C ++ ++ +V + I I ++++L G+ +I+ A R + Y + + + L+ F + VS D + +V ++ NGL S + + E+ + V +Q D K + RD M L NVT L ++ + L +++ ++ + ++ AI V A VE C C G C++ +SGYY+ + IL + C C+G++ C+ V G C C N G C+ C GFY + C+PC CP A NF+ C + E+ C C GYSG CE C G+YGNP + G C CDC N S++ +C+ G C +C G C++CA + V K C++C
Sbjct: 270 CICYGHASSCPWDETTKKLQCQCEHNTCGESCNRCCPGYHQQPWRPGTVSSGNTCEACNCHN---------KAKDCYYDESVAKQKKSLNTAGQF-------------RGGGVCIN-----CLQNTMGINCETCIDGYYRPHKVSPYEDEPCRPCNCDPVGSLSSVCIKDDLHSDLHNGKQPGQCPCKEGYTGEKCDRCQLGYKDYPTCVSCGCNPVGSASDE-----PCTGPCVCKENVEGKACDRCKPGFYNLKEKNPRGCSECFCFGVSDVCSSLSWPVGQVNSMSGWLVTDLISPRKIPSQQDAL-GGRHQVSINNTAVMQR-------LAPKYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETVDSNLMSHADVIIKG-NGL-------TLSTQAEGLSLQPYEEYLNVVRLVPENFQ-DFHSK-----------RQIDRDQLMTVLANVTHLLIRANYNSAKMALYRLESVSLDIA-SSNAIDLVVAADVEHCECPQGYTGTSCESCLSGYYRV----------------DGILFGGICQPCECHGHAAECN-VHGVCIACAHNTTGVHCEQCLPGFYGEPSRGTPGDCQPCACPLTIASNNFSPTC-HLNDGDEVVCDWCAPGYSGAWCERCADGYYGNPTVPGESCVPCDCSGNVDPSEAGHCDSVTGECLKCLGNTDGAHCERCADGFYGDAVTAKNCRAC 902
BLAST of Laminin subunit alpha-2 vs. UniProt/SwissProt
Match: sp|P19137|LAMA1_MOUSE (Laminin subunit alpha-1 OS=Mus musculus OX=10090 GN=Lama1 PE=1 SV=2) HSP 1 Score: 747.273 bits (1928), Expect = 0.000e+0 Identity = 441/1186 (37.18%), Postives = 640/1186 (53.96%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT--HKGY--LEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGE--DIGDQK-PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTF-TGEKIALPK--FTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQ-STLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQ--RQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITL---SRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP-RLDY-QMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK--KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYW--NNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC---YNGQV---CNNE----------GICVCPPNTIGNLCNKCSEDSWDF--NETIGCKKCNCYINGSYSNICNKISG 3556
L NA I+++ATCG GPE+FC+LV+ + + P H C VCD + N +RHPI A D N WWQSPS+ G +Y V VT+DL+QV+Q+ YI++K ++PRPGNWILERS+DG + PW ++A + C Y + R+ P P+ AD+E+ CT+YYS+ L GEI SLINGR S + PQ L+ F SARYIR R + TL ADLMT H+ L+ + RYYYS+++I +GG CIC HA C + +C C+HNTCG +C+ CCP ++Q+PW+P + CEECNC+ K+ +C Y+ +VA + S+N G+ GGGVC+NC +NT GINCE CI +YRP V + +PC PC C +G++ S C+ + D+ + K PG C C+KGY G CD C GY +PN C+PC C GS+ C PC CK +V + C CK G++NL NPEGC C+CFG++ C SL+ +++++ GW +T + KI + + ++ N+ + +R + YYW AP YLGN +T++G +Y V ++ T+ L+ D+IIK + S E LQ + F ++ + +++ ++ L V LLI+A Y S + ++ L +++L S + LA+ +E C CP G+ G SCE+C GY RV+ +L+ G C+ C C+ H++ CD ++G C CTHNT G HCE C G+YG C+ C CP +D + C E VC +C SG C+RC +GYYGNP +C C C+ N + CD VTGEC +C +NT G C+ C GFYGDA+T K+C+ CDC GS S C TG CDC+ + +QCD C SG Y D C+PCNC ++G +S C T GQC C V+G +C++C G++ + + C C C+ +CD +G+C C + C++CE YW + C CNC GS + +CD+ +G+C C + + C +C +GY C PC G + C+ E G C C N +G C+KC ++ + GC C C+ S +C+++ G
Sbjct: 36 LATNAHISANATCGEKGPEMFCKLVEHVPGR----PVRHAQCRVCDGNSTNPRERHPISHAI-DGTNNWWQSPSIQNGREYHWVTVTLDLRQVFQVAYIIIKAANAPRPGNWILERSVDGVKFKPWQYYAVSDTECLTRYKITPRRGP---------PTYRADNEVICTSYYSKLVPLEHGEIHTSLINGRPSADDPS-------PQ-LLEFTSARYIRLRLQRIRTLNADLMTLSHRDLRDLDPIVTRRYYYSIKDISVGGMCICYGHASSCP--WDEEAKQLQCQCEHNTCGESCDRCCPGYHQQPWRPGTISSGNECEECNCHNKAKDCYYDSSVAKERRSLNTAGQYSGGGVCVNCSQNTTGINCETCIDQYYRPHKVSPYDDHPCRPCNCDPVGSLSSVCIKDDRHADLANGKWPGQCPCRKGYAGDKCDRCQFGYRGFPN--CIPCDCRTVGSLNED--PCIEPCLCKKNVEGKN-CDRCKPGFYNLKERNPEGCSECFCFGVSGVCDSLTWSISQVTNMSGWLVTDLMSTNKIRSQQDVLGGHRQISINNTAVMQRLT--STYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETVDSDLMSHADIIIKGNGLTI--STRAEGLSLQPYEEYFNVVRLVPENFRDFDTRREIDRDQLMTVLANVTHLLIRANYNSAKMALYRLDSVSLDIASPNAIDLAV--AADVEHCECPQGYTGTSCEACLPGYYRVDGILFGGICQPCECHGHASECD-IHGICSVCTHNTTGDHCEQCLPGFYGTPSRGTPGDCQPCACPLSIDSNNFSPTCHLTDGEEVVCDQCAPGYSGSWCERCADGYYGNPTVPGGTCVPCNCSGNVDPLEAGHCDSVTGECLKCLWNTDGAHCERCADGFYGDAVTAKNCRACDCHENGSLSGICHLETGLCDCKPHVTGQQCDQCLSGYYGLDTGLGCVPCNCSVEGSVSDN--C-TEEGQCHCGPGVSGKQCDRCSHGFYAFQDGGCTPCDCA--HTQNNCDPASGECLCPPHTQGLKCEECEEA-YWGLDPEQGCQACNCSAVGSTSAQCDVLSGHCPCKKGFGGQSCHQCSLGYRSF-----PDCVPCGCDLRGTLPDTCDLEQGLCSCSEDGGTCSCKENAVGPQCSKCQAGTFALRGDNPQGCSPCFCF---GLSQLCSELEG 1171 HSP 2 Score: 406.757 bits (1044), Expect = 4.121e-112 Identity = 416/1709 (24.34%), Postives = 689/1709 (40.32%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPKKKIS--CKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCD-SLGST--SENCDKLTGK---CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNE------LTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYE----YHIFDEKN--SLESGKDYST--IHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDL-----SLFYRSIFMNVSVDCLDN-------KCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIK-IKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP-SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTL-PSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKV----------LEDLRSTFTDINETISGHEQ---------LGISNELIKNATAYLETIK--NIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMA---DEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQK----LDAI---SKSFQD-------EKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNIT-----RDILAVDYAINQTKKELD----FNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSE----------LIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTG-KFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGF-------PSSKLLRSLKLEGRLISDLKE-NYDNYFYK-----GCLFGVKLMNTEFGL--QDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSN 7300
+C +G C+ C+ GYY C+ C+C+ + CD + G C C++NT G C+ C GFYG C+ C C S+ S S C G+ CD C + C+ C G Y + C+PCNC C++ +G+C CL N +G CE+C +G++ NC+ C C + +LS C L G C C +V + CD+C + +Y + CVPCNC GS++ C E G C C V+ + C +C G++ GC C + C+ G C+CPP+T G C +C E W + GC+ CNC GS S C+ +SG C CK F G C +C+ GY FP+C PC C GT + L C + G C CK+N +G +C C+ GTF G GC CFCFG+S+ C + + ++ H+ + N G + L+A R+ + P Y + K F L L Y F + N K GR + ++M++ G+R D+ K W +Y N + +K + D FM L N+ + +K Y + I I + + A G+ +E C+C T G+ CQ+ GYY+E LP S G +L CNCN +S VCD TG C +C + G+ C+LC G+Y C PC CP ++F+ C +E + +C C GY G+ CE C G++GNP G C++CDCN QGS +C+ G C C PG G+ C++C P +++ + C SC C LL +D++ + V++ + P+ + N N + L+ K + + +++ + Q + ++ + +K+ + +E L + +I E ++ Q + + +N ++ L IK N + N TL KD+L + Q Q Q LK LK + E +E L +K +E L ++L+ +K+ Q+ ++ ++++ + I K +DA + + QD +D + + R+I + +L ++ + ++H + +++ L + L N NA A +SNI ++LA D K +L +R + + L E S + + +D E + L Q++ N K N + + E P G + R L+ E +L ++ + N + D + ++ ++ + + K+KD+ L +RL+ + + E L+ A + V I+ V R + Y P +++ + + + +LF Y S S A + R + F +D G+ +E +N + I IT + ++ S V TS + + I T +FVGG P+ K+ G + K NY + GC + ++ F +E+ R T++ +L F+PN + +L SN
Sbjct: 717 ECPQGYTGTSCEACLPGYYRVDGILFGGICQPCECHGHASECD-IHGICSVCTHNTTGDHCEQCLPGFYGTPSRGTPGDCQPCACPLSIDSNNFSPTCHLTDGEEVVCDQCAPGYSGSWCERCADGYYGNPTVPGGTCVPCNCSGNVDPLEAGHCDSVTGECLKCLWNTDGAHCERCADGFYGDAVTAKNCRACDCHENGSLSGICHLETGLCDCKPHVTGQQCDQCLSGYYGLDTGLGCVPCNCSVEGSVSDNC-TEEGQCHCGPGVSGKQCDRCSHGFYAFQDGGCTPCDCAHTQNNCDPASGECLCPPHTQGLKCEECEEAYWGLDPEQGCQACNCSAVGSTSAQCDVLSGHCPCKKGFGGQSCHQCSLGYRSFPDCVPCGCDLRGTLPDTCDLEQGLCSCSEDGGTCSCKENAVGPQCSKCQAGTFALRGDNPQGCSPCFCFGLSQLCSELEGYVRTLITLASDQPLLHVVSQSNLKGTIEGVHFQPPDTLLDAEAVRQHIYA----EPFYWRLPKQFQGDQLLAYGGKLQYSVAFYSTLGTGTSNYEPQVLIKGGRA--RKHVIYMDAPAPENGVR--------------QDYEVRMKEEFW---KYFNSVSEKHVTHSD------------FMSVLSNIDYILIKASYGQGLQQSRIANISMEVGRKAVELPAEGEAALLLELCVCPPGTAGHSCQDCAPGYYRE--KLPESGGRGP----RPLLAPCVPCNCNNHSDVCDPETGKCLSCRDHTSGDHCELCASGYYGKVTGLPGDCTPCTCPHHPPFSFSPTCV-VEGDSDFRCNACLPGYEGQYCERCSAGYHGNPRAAGGSCQTCDCNPQGSVHSDCDRASGQCVCKPGATGLHCEKCLPRHILMESDCVSCDDDCVGPLLNDLDSVGDAVLSLNLTGVSPAPYGILENLENTTKYFQRYLIKENAKKIRAEIQLEGIAEQTENLQKELTRVLARHQKVNAEMERTSNGTQALATFIEQLHANIKEITEKVATLNQTARKDFQPPVSALQSMHQNISSLLGLIKERNFTEMQQNATLELKAAKDLLSRI----------QKRFQKPQ-EKLKALKEANSLLSNHSEKLQAAEELLKEAGSKTQESNLLLLLVKANLKEFQEKKLRVQEEQNVTSELIAKGREWVDAAGTHTAAAQDTLTQLEHHRDELLLWARKIRSHVDDLVMQMSKRRARDLVHRAEQHASELQSRAGALDRDLENVRNVSLNATSAAHVHSNIQTLTEEAEMLAADAHKTANKTDLISESLASRGKAVLQRSSRFLKESVSTRRKQQGITMKLD-ELKNLTSQFQESMDNIMKQANDSLAMLRESPGGMREKGRKARELAAAANESAVKTLEDVLALSLRVFN-------TSEDLSRVNATVQETNDLLHNSTMTTLLAGRKMKDMEMQANLLLDRLKPLKTLEENLSRNLSEIKLLISRARKQVASIKVAVSADRDCIRAYQPQTSSTNYNTLILNVKTQEPDNLLF-YLGSSSSSDFLAVEMRR-GKVAFLWDLGSGSTRLEFPEVSINNNRWHSIYITRFGNMGSLSVKEASAAENPPVRTSK-SPGPSKVLDINNSTLMFVGGLGGQIKKSPAVKVTHFKGCMGEAFLNGKSIGLWNYIEREGKCNGCFGSSQNEDSSFHFDGSGYAMVEKTLRPTVTQIVILFS----TFSPNGLLFYLASN 2355 HSP 3 Score: 235.728 bits (600), Expect = 3.064e-60 Identity = 185/670 (27.61%), Postives = 276/670 (41.19%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLG-TIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSV------RTSFYRCKP---PCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVY----YWTAPPPYLGNWITSYGTIFRYVVKIELQSTL-KGSLIDSPDVIIKCGDFR---LFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLAL----DEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQ--------CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP-RLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLC--KHGFYGDALTKSCKKCDCDSLGSTSENCDKL 2848
C C+ D E G +C+C G C+ C F C C+C N C G C+ C +T+G+ CE+C + + W + + C C C +G T C D G C CKKG+ G +C CS+GY +P +C+PC C + G++ C C+CK + C C+ G F L NP+GC PC+CFG++ CS L + L + + L + ++ +D +Y YW P + G+ + +YG +Y V STL G+ P V+IK G R ++ + +++ + +K WK +K T +F+S L +D +LIKA Y + + NI++ G + L + +E C CPPG G SC+ C GY R G+ C C C NHS+ CD G+C +C +T+G HCE CASGYYG C C CP + + C ++ C+ CL G +C+RC GY+GNP+ SC+ C CN CD +G+C C G C+ C +H + C CD D +G + D +
Sbjct: 958 CNCSVEGSVSDNCTEEG----QCHCGPGVSGKQCDRCSHGFYAFQ---DGGCTPCDCAHTQNNCD------------------PASGECL-CPPHTQGLKCEECEEAY---WGLDPEQ--GCQACNCSAVGSTSAQC--------DVLSGHCPCKKGFGGQSCHQCSLGYRSFP--DCVPCGCDLRGTLPDTCDLEQGLCSCSEDGGTCSCKENAVGPQ-CSKCQAGTFALRGDNPQGCSPCFCFGLSQLCSELEGYVRTLITLASDQPLLHVVSQSNLKGTIEGVHFQPPDTLLDAEAVRQHIYAEPFYWRLPKQFQGDQLLAYGGKLQYSVA--FYSTLGTGTSNYEPQVLIKGGRARKHVIYMDAPAPENGVRQDYEVRMKEEFWKYFNSVSEKHVTHSDFMSVLSNIDYILIKASYGQGLQQSRIANISMEVGRKAVELPAEGEAALLLELCVCPPGTAGHSCQDCAPGYYREKLPESGGRGPRPLLAPCVPCNCNNHSDVCDPETGKCLSCRDHTSGDHCELCASGYYGKVTGLP-GDCTPCTCPHHPPFSFSPTCVVEGDSDFRCNACLPGYEGQYCERCSAGYHGNPRAAGGSCQTCDCNPQGSVHSDCDRASGQCVCKPGATGLHCEKCLPRHIL----MESDCVSCDDDCVGPLLNDLDSV 1578 HSP 4 Score: 152.91 bits (385), Expect = 4.761e-35 Identity = 185/749 (24.70%), Postives = 282/749 (37.65%), Query Frame = 2
Query: 3086 CLCSPHALSA--DCDLNNGKCKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIG-----CKKCNCYINGSYSNIC-----------NKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAI---------LNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTA-VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILIN--LKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPSA--HYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKG---IPC--------------------------------------------------KSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNY 5038
C+C HA S D + +C+C N CD+C + W T +C CNC + +C +VA+ S G + G VC N C NT G C C + + ++ C+ CNC GS S++C K G C C+ + G KCDRC GY GFPNC PC C G+ + D C CK+NV G C C+ G + GC CFCFGVS C ++ ++ +V + + I +++ L G +I+ A R + Y + + + L+ F + VS D ++ ++ ++ NGL S + + E+ + V +++ + I + + +N+T ++ N S K+ LY ++ ++ + + AI V A VE C C G C+ + GYY+ + IL + C C+G++ CD + G C C N G+ C+ C GFY C+PC CP + NF+ C + E+ C QC GYSG CE C G+YGNP + G +PC ++CDC++ GS S C+ E G+C C P V G +CDQC Y
Sbjct: 277 CICYGHASSCPWDEEAKQLQCQCEHNTCGESCDRCCPGYHQQPWRPGTISSGNECEECNCHN---------KAKDCYYDSSVAKERRSLNTAGQYS-------------GGGVCVN-----CSQNTTGINCETCIDQYYRPHKVSPYDDHPCRPCNCDPVGSLSSVCIKDDRHADLANGKWPGQCPCRKGYAGDKCDRCQFGYRGFPNCIPCDCRTVGSLNE-----DPCIEPCLCKKNVEGKNCDRCKPGFYNLKERNPEGCSECFCFGVSGVCDSLTWSISQVTNMSGWLVTDLMSTNKIRSQQDVL-GGHRQISINNTAVMQR-------LTSTYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETVDSDLMSHADIIIKG-NGL-------TISTRAEGLSLQPYEEYFNVVRLVPENFRDFDTRREIDRDQLMTVLANVTH---LLIRANYNSAKMALY---------RLDSVSLDIA-SPNAIDLAVAADVEHCECPQGYTGTSCEACLPGYYRV----------------DGILFGGICQPCECHGHASECD-IHGICSVCTHNTTGDHCEQCLPGFYGTPSRGTPGDCQPCACPLSIDSNNFSPTC-HLTDGEEVVCDQCAPGYSGSWCERCADGYYGNPTVPGGTCVPCNCSGNVDPLEAGHCDSVTGECLKCLWNTDGAHCERCADGFYGDAVTAKNCRACDCHENGSLSGICHLETGLCDCKPHVTGQQCDQCLSGY 946
BLAST of Laminin subunit alpha-2 vs. UniProt/SwissProt
Match: sp|Q60675|LAMA2_MOUSE (Laminin subunit alpha-2 OS=Mus musculus OX=10090 GN=Lama2 PE=1 SV=2) HSP 1 Score: 725.317 bits (1871), Expect = 0.000e+0 Identity = 443/1172 (37.80%), Postives = 628/1172 (53.58%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLM--THKG--YLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQ--KPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFT---SFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSL-IDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAA----------TKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDE--MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQE-----SCDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSC---TSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV-TGCKKCPPCYNGQVCNNE----------GICVCPPNTIGNLCNKCSEDSW--DFNETIGCKKCNCY 3514
L NA IT++ATCG GPE++C+LV+ + Q + NP C +C+ + N +RHPI A D N WWQSPS+ G++Y V +T+DL+QV+QI Y+++K +SPRPGNWILERSLD Y PW + A T C YN++ R P PS DDE+ CT++YS+ L +GEI ISLINGR S + P+ L+ F SARYIR RF + TL ADLM HK ++ + RYYYS+++I +GG CIC HA C ++ + ++C C+HNTCG +C+ CCP F+Q+PW+ CE CNC+ K+ EC Y++TVA+ LS+N G+ GGGVCINC NT GINCE C+ GF+RP V PC PC C G++ E + KPG C CK G+ GVNCD C GY YP +C PC CS GS T+ C PC+CK +V +D C CK+G+FNL N +GCE C+C G++N+C S I D++GW LT +G P+ S Q ++ NS + R + YYW+APPPYLGN + + G + + +L+ + I +I + D R+ S + ++ +Y++P+E I +++ + A T+++F+ L ++++L++ Y +I L+ V D +E C CPPG++G SCE+C +RRVN ++ G CE C C+ H+ CD + GEC NC +T G +C +C G+YGD + C+ C CP + C S +C +C +G C+RC EGY+G P SC+ C+CN+N + SCD ++G C C T G C+LC G++GDA+ K+C+ C C+ GS SE C TG+C+CR N R CD C T GL L + C+PCNC G S+ F SGQC+C V G +C++C GYFN+ C C CS L +CD G+C C N C +C + W + T C CNC GSL +C++ TG C C + CS+C G++ + T C P + C+ E G C C N G C++C + D +GC C C+
Sbjct: 42 LASNALITTNATCGEKGPEMYCKLVEHVPGQPVRNPQ----CRICNQNSSNPYQRHPITNA-IDGKNTWWQSPSIKNGVEYHYVTITLDLQQVFQIAYVIVKAANSPRPGNWILERSLDDVEYKPWQYHAVTDTECLTLYNIYPRTGP---------PSYAKDDEVICTSFYSKIHPLENGEIHISLINGRPSADDPS-------PE-LLEFTSARYIRLRFQRIRTLNADLMMFAHKDPREIDPIVTRRYYYSVKDISVGGMCICYGHARACP--LDPATNKSRCECEHNTCGESCDRCCPGFHQKPWRAGTFLTKSECEACNCHGKAEECYYDETVASRNLSLNIHGKYIGGGVCINCTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTGSLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYP--DCQPCNCSGLGS--TNEDPCVGPCSCKENVEGED-CSRCKSGFFNLQEDNQKGCEECFCSGVSNRCQSSYWTYGNIQDMRGWYLTDLSGRIRMAPQLDNPDSPQQISISNS--EARKSLLDGYYWSAPPPYLGNRLPAVGGQLSFTISYDLEEEEDDTEKILQLMIIFEGNDLRI--STAYKE--------VYLEPSEEHIEEVSLKEEAFTIHGTNLPVTRKDFMIVLTNLERVLMQITYNLGMDAIFRLSSVNLESAVPYPTDRRIATDVEVCQCPPGYSGSSCETCWPRHRRVNGTIFGGICEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRCNINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQL-GRGCLPCNCNSFG---SKSFDCEASGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIACDCS--HLGNNCDPKTGQCICPPNTTGEKCSECLP-NTWGHSIVTGCKVCNCSTVGSLASQCNVNTGQCSCHPKFSGMKCSECSRGHWNYPLCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCRPGKFGLDAKNPLGCSSCYCF 1165 HSP 2 Score: 226.098 bits (575), Expect = 3.098e-57 Identity = 156/425 (36.71%), Postives = 206/425 (48.47%), Query Frame = 2
Query: 2654 CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSEN------CDKLTGK-CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKC--ENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSN----ELTK--CDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
C+ C+C + E+CD +TGEC C +T G C C GFYGD S C+ C C L S N D+ G CD C + +C+ C G + + C PC C S C++ SG C C G CE C +GYF N NC+ C C+ + + S C G+C+C NV R CD+C E R C+PCNC GS + C+ +G C C VA + C +C GYF GC C + G C+ + G C+CPPNT G C++C ++W + GCK CNC GS ++ CN +G C+C F G KC C+ G++ +P C C C GT + E K C TGQC CK NV G C CR G F D GC SC+CFGV+ +C
Sbjct: 750 CEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCAC-PLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRCNINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQLGR-GCLPCNCNSFGSKSFDCEA-SGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIACDCSHLGNNCDPKTGQCICPPNTTGEKCSECLPNTWGHSIVTGCKVCNCSTVGSLASQCNVNTGQCSCHPKFSGMKCSECSRGHWNYPLCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCRPGKFGLDAKNPLGCSSCYCFGVTSQC 1171 HSP 3 Score: 222.246 bits (565), Expect = 4.749e-56 Identity = 198/664 (29.82%), Postives = 280/664 (42.17%), Query Frame = 2
Query: 1007 VAKCNCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCN---------RKSNECSYNQTVATLQLSVNKMGRM---EGG-----------------GVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTS----FYRCK-----PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKF-TSFQYLTSENSYIDERFNNPTV---------------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCG---DFRLF--HSVSIEDRRLQRQVFIYIKPTEWKIGKLTP--DKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQ--MTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQE---SCDPVTGECRCSYNTMGTECQLCKH 2761
+C C+ N G +C+ C P F + + C CNCN S +C VA + G EGG G CI C NT G C +C+ + V C C C +G++ S C N G C C + G+ C CS G+ YP C C C + G+ T+ +C C+CKV+V C C+ G F LD+ NP GC CYCFG+T+QCS AK L ++ W T + E+ LP + Q+ T++ F P + +YW P + G + +YG +Y + E + G P VII+ G R+ H + +L R I + EWK P + T+E+F+ L + +LIKA Y + + + I++ E L P IE+C+CPPG++GLSCE+C G+ R+ + G C C C HS+ CD CQNC H+TAG CE CA GYYG + N C+ C CP + + C E+Y C C G +C+RC GY G+P SC+ C+C+ CD VTG C C G +C C+H
Sbjct: 930 TGQCECRPNVQGRHCDECKPETFGLQLGR---GCLPCNCNSFGSKSFDCEASGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIACDCSHLGNNCDPKTGQCI-CPPNTTGEKCSECLPNTWGHSIVTG-----CKVCNCSTVGSLASQCNVN--------TGQCSCHPKFSGMKCSECSRGHWNYP--LCTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVH-CDRCRPGKFGLDAKNPLGCSSCYCFGVTSQCSE--AKGL----IRTW--VTLSDEQTILPLVDEALQHTTTKGI----AFQKPEIVAKMDEVRQELHLEPFYWKLPQQFEGKKLMAYGGKLKYAIYFEARDE-TGFATYKPQVIIRGGTPTHARIITRHMAAPLIGQLTRHE-IEMTEKEWKYYGDDPRISRTVTREDFLDILYDIHYILIKATYGNVVRQSRISEISMEVAEPGHVLAGSPPAHLIERCDCPPGYSGLSCETCAPGFYRLRSEPGGRTPGPTLGTCVPCQCNGHSSQCDPETSVCQNCQHHTAGDFCERCALGYYGIVRGLP-NDCQPCACPLISPSNNFSPSCVLEGLEDYRCTACPRGYEGQYCERCAPGYTGSPSSPGGSCQECECDPYGSLPVPCDRVTGLCTCRPGATGRKCDGCEH 1558 HSP 4 Score: 146.362 bits (368), Expect = 3.566e-33 Identity = 163/651 (25.04%), Postives = 268/651 (41.17%), Query Frame = 2
Query: 4289 DSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTA--VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKIN--------ISIQMSSELLNSINK--FYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTD----------INETISGHEQLGISN--ELIKNATAYLETIKN----IYKEMPNITLAAS--VLKDVLKYVDELNQIIKYTQSHIQS----------DQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKEL 6097
D I+ +TR+ F+ L ++ + +K Y + V +I I+ +V E + A +E+C C G C+ G+Y+ S G RT L C CNG+S CD T CQNC + G+ C+ C G+Y + + C+PC CP S NF+ +C +E + +C C GY G+ CE C G+ G+P G C+ C+C+ GS + C+ G+C C PG G KCD C + E C C CT LL + L + +N LP+ + N +S Q + E L + + L E + K+ D ++ + E+ +R + E ++ D +NET+ ++ N EL K L+ +++ KE+ L A+ +LK V K E + + +Q D + L+ +KT A+ ++ N++ + + TKK+ E I +K+ I + + + I +D I + +S +I+DL + + L K + H Q + S+ L L + N NA A AYSNI +D + + + KEL
Sbjct: 1312 DDPRISRTVTREDFLDILYDIHYILIKATYGNVVRQS--RISEISMEVAEPGHVLAGSPPAHLIERCDCPPGYSGLSCETCAPGFYRL-----RSEPGG--RTPGPTLGTCVPCQCNGHSSQCDPETSVCQNCQHHTAGDFCERCALGYYGIVRGLPNDCQPCACPLISPSNNFSPSCV-LEGLEDYRCTACPRGYEGQYCERCAPGYTGSPSSPGGSCQECECDPYGSLPVPCDRVTGLCTCRPGATGRKCDGCEHWHAREGAECVFCGDECTGLLLGDLARLEQMTMNINLTGPLPAPYKILYGLENTTQELKHLLSPQRAPERLIQLAEGNVNTLVMETNELLTRATKVTADGEQTGQDAERTNSRAESLEEFIKGLVQDAEAINEKAVQLNETLGNQDKTAERNLEELQKEIDRMLKELRSKDLQTQKEVAEDELVAAEGLLKRVNKLFGEPRAQNEDMEKDLQQKLAEYKNKLDDAWDLLREATDKTRDANRLSAANQKNMTILETKKEAIEGSKRQIENTLKEGNDILDEANRLLGEINSVIDYVDDIKTKLPPMSEELS---DKIDDLAQEIKDRRLAEKVF----QAESHAAQLNDSSAVLDGILDEAKNISFNATAAFRAYSNI-KDYIDEAEKVAREAKEL 1944 HSP 5 Score: 103.605 bits (257), Expect = 3.268e-20 Identity = 88/275 (32.00%), Postives = 118/275 (42.91%), Query Frame = 2
Query: 3086 CLCSPHALSADCD--LNNGKCKCGSNVINRICDKC-ENLHY--WN-----NRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHA 3838
C+C HA + D N +C+C N CD+C H W +++C CNC + C + VA R+ S L++ G G VC N C NT G C C + + N C+ C+C GS S +C K G C CKT F G CDRC GY+G+P+CQPC+C+ G+ +NE D G C CK+NV G C C+ G F + GC CFC GVS +C +
Sbjct: 283 CICYGHARACPLDPATNKSRCECEHNTCGESCDRCCPGFHQKPWRAGTFLTKSECEACNC---------HGKAEECYYDETVASRNLS--------LNIHG-----KYIGGGVCIN-----CTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTGSLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYPDCQPCNCSGLGS-TNE----DPCVGPCSCKENVEGEDCSRCKSGFFNLQEDNQKGCEECFCSGVSNRCQS 525 HSP 6 Score: 97.8265 bits (242), Expect = 1.893e-18 Identity = 78/268 (29.10%), Postives = 121/268 (45.15%), Query Frame = 2
Query: 4304 NSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSL--NCNEF-GICQ-CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
N +TR FM+ L N+ + +++ + + ++ + + + T VE C C G+ C+ + + N T G + + C C +++ CD +TG+C NC + G C C GFY + + + C+PC CP NF+ C ++ SL L C +C IGY+G CE C +G++G P I G C+ C CN S+ +C+ G C C PG G C+ CA Y V K CQ C
Sbjct: 662 NLPVTRKDFMIVLTNLERVLMQITYNLGMDAIFRLSSVNLESAVPYPTDRRIATDVEVCQCPPGYSGSSCETCWPRHRRV-----NGTIFGGI---------CEPCQCFAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTC-HLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPC 914 HSP 7 Score: 88.5817 bits (218), Expect = 1.391e-15 Identity = 49/142 (34.51%), Postives = 75/142 (52.82%), Query Frame = 2
Query: 4646 CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSC 5068
CDS++G C C G C+LC +G++ + N C+PC+C N + + I + +C+CR G+ C+ C+ +G + G C C+CN GS+S +C G C C PGV G KCD+CA Y ++ C +C
Sbjct: 875 CDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRC-----NINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQL-GRGCLPCNCNSFGSKSFDCEASGQCWCQPGVAGKKCDRCAHGYFNFQEGGCIAC 1010 HSP 8 Score: 69.3218 bits (168), Expect = 9.409e-10 Identity = 64/237 (27.00%), Postives = 88/237 (37.13%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY---GNPKKKI------SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN-----CKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCN 3232
C SG+ C+ C G+Y P + +C C+CN + CDP T C+ C ++T G C+ C G+YG L C+ C C L S S N + C L+D +C C G CE+C GY P+ C++C C P+ +L CD G C C R CD CE+ H +CV C
Sbjct: 1378 CPPGYSGLSCETCAPGFYRLRSEPGGRTPGPTLGTCVPCQCNGHSSQCDPETSVCQNCQHHTAGDFCERCALGYYGIVRGLPNDCQPCAC-PLISPSNN---FSPSCVLEG---------------LEDYRCTACP-----------------------RGYEGQYCERCAPGYTGSPSSPGGSCQECECDPYGSLPVPCDRVTGLCTCRPGATGRKCDGCEHWHAREG-AECVFCG 1571 HSP 9 Score: 61.2326 bits (147), Expect = 2.264e-7 Identity = 44/146 (30.14%), Postives = 64/146 (43.84%), Query Frame = 2
Query: 3218 CVPCNCGRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQV---------CNNEGI-----CVCPPNTIGNLCNKCSEDSWDFNETIG--CKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRC 3604
CVPC C S +CD ET C+ C + A C +C +GY+G+ C PC + C EG+ CP G C +C+ + G C++C C GS C++++G+C C+ G KCD C
Sbjct: 1413 CVPCQCNGHS--SQCDPETSVCQNCQHHTAGDFCERCALGYYGIVRGLPNDCQPCACPLISPSNNFSPSCVLEGLEDYRCTACPRGYEGQYCERCAPGYTGSPSSPGGSCQECECDPYGSLPVPCDRVTGLCTCRPGATGRKCDGC 1556 HSP 10 Score: 59.6918 bits (143), Expect = 7.699e-7 Identity = 60/239 (25.10%), Postives = 103/239 (43.10%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKN---DKNYDQ-CEPCQC-PSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE---FGICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCY 5071
T +C C T G C G++++ + ++G+ T + + CNC+G ++ C ++V G C NC N G C+ C +GF++ NY + C+PC C P+ + ++ C + E + C C+ G+ G +C+ C +G++G P C+ C+C+ GS NE G C C V G C +C + +K C+ C+
Sbjct: 297 ATNKSRCECEHNTCGESCDRCCPGFHQKPW------RAGTFLTKSEC----EACNCHGKAEECYYDETVASRNLSLNIHGKYIGGGVCINCTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTG--SLSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGYP----DCQPCNCSGLGST----NEDPCVGPCSCKENVEGEDCSRCKSGFFNLQEDNQKGCEECF 515
BLAST of Laminin subunit alpha-2 vs. UniProt/SwissProt
Match: sp|P24043|LAMA2_HUMAN (Laminin subunit alpha-2 OS=Homo sapiens OX=9606 GN=LAMA2 PE=1 SV=4) HSP 1 Score: 715.687 bits (1846), Expect = 0.000e+0 Identity = 433/1176 (36.82%), Postives = 614/1176 (52.21%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLM--THKG--YLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQ--KPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPK---FTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGS--------LIDSPDVIIKCGDFRLFHSVSIEDRR--LQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDE--MPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQE-----SCDPVTGECR-CSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR--TDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYF-------------GLDVTGC----KKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW--DFNETIGCKKCNCY 3514
L NA IT++ATCG GPE++C+LV+ + Q + NP C +C+ + N +RHPI A D N WWQSPS+ G++Y V +T+DL+QV+QI Y+++K +SPRPGNWILERSLD Y PW + A T C YN++ R P PS DDE+ CT++YS+ L +GEI ISLINGR S + P+ L+ F SARYIR RF + TL ADLM HK ++ + RYYYS+++I +GG CIC HA C ++ + ++C C+HNTCG +C+ CCP F+Q+PW+ CE CNC+ K+ EC Y++ VA LS+N G+ GGGVCINC +NT GINCE C GF+RP V PC PC C +G++ E + PG C CK G+ GV+CD C+ GY YP +C C CS GS C PC CK +V D C CK+G+FNL N +GC+ C+C G++N+C S KI D+ GW LT G P+ S Q ++ N+ + R P YYW+AP PYLGN + + G + + +L+ + + +++ D+ I ++ S E L ++ I T + + ++EF++ L + ++L++ Y +I L+ V D +E C CPPG+ G SCESC +RRVN ++ G CE C C+ H+ +CD + GEC NC +T G +C+ C G+YG+ C+ C CP + C S +C C +G C+RC EGY+G P SC+ C+CN+N + SCD ++G C C T G C+LC G++GDA+ K+C+ C C++ GS SE C TG+C+CR N ++CD C +G + + C+PCNC G S+ F SGQC+C V G +C++C GYFN+ C C CS L +CD G+C C N I C KC + W + T C CNC GSL+ +C++ TG C C + C++C G++ G D T C KKC + G C C N G C++C + D +GC C C+
Sbjct: 46 LASNALITTNATCGEKGPEMYCKLVEHVPGQPVRNPQ----CRICNQNSSNPNQRHPITNA-IDGKNTWWQSPSIKNGIEYHYVTITLDLQQVFQIAYVIVKAANSPRPGNWILERSLDDVEYKPWQYHAVTDTECLTLYNIYPRTGP---------PSYAKDDEVICTSFYSKIHPLENGEIHISLINGRPSADDPS-------PE-LLEFTSARYIRLRFQRIRTLNADLMMFAHKDPREIDPIVTRRYYYSVKDISVGGMCICYGHARACP--LDPATNKSRCECEHNTCGDSCDQCCPGFHQKPWRAGTFLTKTECEACNCHGKAEECYYDENVARRNLSLNIRGKYIGGGVCINCTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLNEVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYP--DCKACNCSGLGSKNED--PCFGPCICKENVEGGD-CSRCKSGFFNLQEDNWKGCDECFCSGVSNRCQSSYWTYGKIQDMSGWYLTDLPGRIRVAPQQDDLDSPQQISISNA--EARQALPHSYYWSAPAPYLGNKLPAVGGQLTFTISYDLEEEEEDTERVLQLMIILEGNDLSISTAQDEVYLHPSEEHTNVLLLKEESFTIHGTHFPV---------RRKEFMTVLANLKRVLLQITYSFGMDAIFRLSSVNLESAVSYPTDGSIAAAVEVCQCPPGYTGSSCESCWPRHRRVNGTIFGGICEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRCNAGGSFSEVCHSQTGQCECRANVQGQRCDKCKAGTFGLQSARGCVPCNCNSFG---SKSFDCEESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTACECS--HLGNNCDPKTGRCICPPNTIGEKCSKCAP-NTWGHSITTGCKACNCSTVGSLDFQCNVNTGQCNCHPKFSGAKCTECSRGHWNYPRCNLCDCFLPGTDATTCDSETKKC------SCSDQTGQCTCKVNVEGIHCDRCRPGKFGLDAKNPLGCSSCYCF 1169 HSP 2 Score: 234.187 bits (596), Expect = 1.017e-59 Identity = 156/424 (36.79%), Postives = 209/424 (49.29%), Query Frame = 2
Query: 2654 CKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCDSLGSTSEN------CDKLTGK-CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYF----NYPNCKKCLC-SPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKS----NELTK--CDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
C+ C+C + ESCD VTGEC C +T G C C GFYG+ ++ C+ C C L S N D+ G CD C + +C+ C G + + C PC C S C++ SG C C G CE C +GYF + NC+ C C + + S C G+C+C +NV + CDKC+ + + CVPCNC GS + C+ E+G C C V + C +C GYF GC C + G C+ + G C+CPPNTIG C+KC+ ++W + T GCK CNC GS CN +G C C F G+KC C+ G++ +P C C C GT + +E K C TGQC CK NV G C CR G F D GC SC+CFG + +C
Sbjct: 754 CEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCAC-PLNIPSNNFSPTCHLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRCNAGGSFSEVCHSQTGQCECRANVQGQRCDKCKAGTFGLQSARGCVPCNCNSFGSKSFDCE-ESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTACECSHLGNNCDPKTGRCICPPNTIGEKCSKCAPNTWGHSITTGCKACNCSTVGSLDFQCNVNTGQCNCHPKFSGAKCTECSRGHWNYPRCNLCDCFLPGTDATTCDSETKKCSCSDQTGQCTCKVNVEGIHCDRCRPGKFGLDAKNPLGCSSCYCFGTTTQC 1175 HSP 3 Score: 221.09 bits (562), Expect = 1.020e-55 Identity = 198/654 (30.28%), Postives = 273/654 (41.74%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCC-PLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIG-SCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTS----FYRCK-----PPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKF-TSFQYLTSENSYIDERFNNPTV---------------YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGD-----FRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTP--DKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH---IEKCNCPPGFNGLSCESCESGYRRVNNVL-------YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQ--MTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP-KKKISCKRCKCNNNQE---SCDPVTGECRCSYNTMGTECQLCKH 2761
C CN+ K E+G +C C+ G C+ C FN + C C C+ N C K GR CI C NT G C KC W T C C C +G++ C N G C C + G C CS G+ YP C C C + G+ T+ +C C CKV+V C C+ G F LD+ NP GC CYCFG T QCS AK L ++ W T E+ LP + Q+ T++ F +P + +YW P + G + +YG +Y + E + S + P VII+ G + H + +L R I + EWK P + T+E+F+ L + +LIKA Y + + + I++ E P IEKC+CP G++GLSCE+C G+ R+ + G C C C HS+ CD CQNC H+TAG CE CA GYYG K N C++C CP + + C + ++Y C C G +C+RC GY G+P SC+ C+C+ CDPVTG C C G +C CKH
Sbjct: 967 CNCNSFGSKSFDCEESG----QCWCQPGVTGKKCDRCAHGYFNFQE----GGCTACECSHLGNNCD------------PKTGR------CI-CPPNTIGEKCSKCAP---NTWGHSITT--GCKACNCSTVGSLDFQCNVN--------TGQCNCHPKFSGAKCTECSRGHWNYP--RCNLCDCFLPGTDATTCDSETKKCSCSDQTGQCTCKVNVEGIH-CDRCRPGKFGLDAKNPLGCSSCYCFGTTTQCSE--AKGL----IRTW--VTLKAEQTILPLVDEALQHTTTKGIV----FQHPEIVAHMDLMREDLHLEPFYWKLPEQFEGKKLMAYGGKLKYAIYFEAREETGFSTYN-PQVIIRGGTPTHARIIVRHMAAPLIGQLTRHE-IEMTEKEWKYYGDDPRVHRTVTREDFLDILYDIHYILIKATYGNFMRQSRISEISMEVAEQGRGTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLP-NDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKH 1562 HSP 4 Score: 137.117 bits (344), Expect = 2.719e-30 Identity = 167/688 (24.27%), Postives = 291/688 (42.30%), Query Frame = 2
Query: 4289 DSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTA----VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-DQCEPCQCP--SAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI-KGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWK-TLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLG-ISNELIKNATAYLETIKNIYKEMPNITLA-----ASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLK-ILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISK----SFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVE-------------QQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSY 6244
D ++ +TR+ F+ L ++ + +K Y + + +I I+ +V E Q G +T +EKC C G C+ + G+Y+ S G RT L C CNG+S +CD T CQNC + G+ C+ C G+Y K + C+ C CP S+ NF+ +C E + +C C GY G+ CE C G+ G+P G C+ C+C+ GS + C+ G C C PG G KCD C + E C C CT LL + L + V++ LP+ + N++ ++ LL+ +L Q + + E+ +L TR KV D T D T + + LG EL ++A A E + + + A + K++ + + EL + TQ I D++ + +LK + E N+E + K +++ ++ D + R+ T D +R+ +L A+++ + + +K+ + R+IE+ +K N ++ ++ + D + ++ ++LN + + + KD QA E+++ D AV I K + FN + A F + NIK+ E EK+ K+ K+ ++
Sbjct: 1316 DDPRVHRTVTREDFLDILYDIHYILIKATYGNFMRQS--RISEISMEVAE--QGRGTTMTPPADLIEKCDCPLGYSGLSCEACLPGFYRL-----RSQPGG--RTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCV-AEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKHWHAREGWECVFCGDECTGLLLGDLARLEQMVMSINLTGPLPAPYKMLYGLENMTQEL-KHLLSPQRAPERL----------IQLAEGNLNTLVTEMNELLTRATKVTADGEQTGQDAERTNTRAKSLGEFIKELARDAEAVNEKAIKLNETLGTRDEAFERNLEGLQKEIDQMIKELRRKNLETQKEIAEDELVAAEALLKKVKKLFGESRGENEEMEKDLREKLADYK-------NKVDDAWDLLREAT---DKIREA-NRLFAVNQKNMTALEKKKEAVESGKRQIENTLK--EGNDILDEANRLADEINSIIDYVEDIQTKLPPMSEELNDKIDDLSQEIKDRKLAEKVSQA-ESHAAQLNDSSAVLDGILDEAKNISFNAT-----------AAFKA-----YSNIKDYIDEAEKVAKEAKDLAH 1950 HSP 5 Score: 100.908 bits (250), Expect = 2.551e-19 Identity = 87/275 (31.64%), Postives = 117/275 (42.55%), Query Frame = 2
Query: 3086 CLCSPHALSADCD--LNNGKCKCGSNVINRICDKC-ENLHY--WN-----NRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNICNK---------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHA 3838
C+C HA + D N +C+C N CD+C H W +T+C CNC + C +NVARR+ S L++ G G VC N C NT G C C++ + N C+ C+C GS + +C K G C CKT F G CDRC GY G+P+C+ C+C+ G+K+ D G C CK+NV G C C+ G F GC CFC GVS +C +
Sbjct: 287 CICYGHARACPLDPATNKSRCECEHNTCGDSCDQCCPGFHQKPWRAGTFLTKTECEACNC---------HGKAEECYYDENVARRNLS--------LNIRG-----KYIGGGVCIN-----CTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLNEVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYPDCKACNCSGLGSKNE-----DPCFGPCICKENVEGGDCSRCKSGFFNLQEDNWKGCDECFCSGVSNRCQS 529 HSP 6 Score: 99.3673 bits (246), Expect = 6.364e-19 Identity = 80/281 (28.47%), Postives = 125/281 (44.48%), Query Frame = 2
Query: 4274 FIILEDSNVINSN---LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY--KNDKNYDQCEPCQCP--SAHYNFAQNCTRIESSLELKCQ-CRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSL--NCNEF-GICQ-CSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
++ E+S I+ + R FM L N+ + +++ + ++ + + + G AVE C C G+ C++ + + N T G + + C C G+++ CD VTG+C NC + G C C GFY + C+PC CP NF+ C ++ SL L C C +GY+G CE C +G++G P + G C+ C CN S+ +C+ G C C PG G C+ CA Y V+ K CQ C
Sbjct: 653 LLLKEESFTIHGTHFPVRRKEFMTVLANLKRVLLQITYSFGMDAIFRLSSVNLESAVSYPTDGSIAAAVEVCQCPPGYTGSSCESCWPRHRRV-----NGTIFGGI---------CEPCQCFGHAESCDDVTGECLNCKDHTGGPYCDKCLPGFYGEPTKGTSEDCQPCACPLNIPSNNFSPTC-HLDRSLGLICDGCPVGYTGPRCERCAEGYFGQPSVPGGSCQPCQCNDNLDFSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPC 918 HSP 7 Score: 94.3597 bits (233), Expect = 2.173e-17 Identity = 52/143 (36.36%), Postives = 80/143 (55.94%), Query Frame = 2
Query: 4646 CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSC 5068
CDS++G C C G C+LC +G++ + + C+PC+C +A +F++ C S +C+CR G+ C+ C+ G +G +G C C+CN GS+S +C E G C C PGV G KCD+CA Y ++ C +C
Sbjct: 879 CDSLSGSCLICKPGTTGRYCELCADGYFGDAVDAKNCQPCRC-NAGGSFSEVC----HSQTGQCECRANVQGQRCDKCKAGTFGLQSARG--CVPCNCNSFGSKSFDCEESGQCWCQPGVTGKKCDRCAHGYFNFQEGGCTAC 1014 HSP 8 Score: 69.707 bits (169), Expect = 6.959e-10 Identity = 60/225 (26.67%), Postives = 88/225 (39.11%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY---GNPKKKI------SCKRCKCNNNQESCDPVTGECR-CSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLH 3196
C SG+ C+ C+ G+Y P + +C C+CN + CDP T C+ C ++T G C+ C G+YG L C++C C + S++ NF SC + L D +C C G CE+C GY P +C++C C P+ +L CD G C C R CD C++ H
Sbjct: 1382 CPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSN--------------NF----SPSCVAE-GLDDYRCTACP-----------------------RGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGCKHWH 1564 HSP 9 Score: 63.1586 bits (152), Expect = 6.770e-8 Identity = 61/224 (27.23%), Postives = 96/224 (42.86%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKN---DKNYDQ-CEPCQC-PSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE---FGICQCSPGVLGIKCDQCAPNY 5038
T +C C T G+ C G++++ + ++G+ T + CNC+G ++ C ++V G C NC N G C+ C +GF++ NY + C+PC C P N + C + E C C+ G+ G SC+ C +G+ G P CK+C+C+ GS+ NE FG C C V G C +C +
Sbjct: 301 ATNKSRCECEHNTCGDSCDQCCPGFHQKPW------RAGTFLTKTEC----EACNCHGKAEECYYDENVARRNLSLNIRGKYIGGGVCINCTQNTAGINCETCTDGFFRPKGVSPNYPRPCQPCHCDPIGSLN--EVCVKDEKHARRGLAPGSCHCKTGFGGVSCDRCARGYTGYP----DCKACNCSGLGSK----NEDPCFGPCICKENVEGGDCSRCKSGF 504 HSP 10 Score: 58.5362 bits (140), Expect = 1.468e-6 Identity = 56/201 (27.86%), Postives = 84/201 (41.79%), Query Frame = 2
Query: 3236 GRGS-LNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCP--------PCY-NGQ--VCNNE-GICV-CPPNTIGNLCNKCSEDSWDFNETI--GCKKCNCYINGSYSNICNK--ISGI-----CACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCR 3754
GRG+ + P D+ C C + C C G++ L + P PC NG +C+ E IC C +T G+ C +C+ + + + C++C C + S +N G+ AC + G C+RC GY G P +CQ C C G+ CD +TG C C+ G KC C+
Sbjct: 1365 GRGTTMTPPADL-IEKCDCPLGYSGLSCEACLPGFYRLRSQPGGRTPGPTLGTCVPCQCNGHSSLCDPETSICQNCQHHTAGDFCERCALGYYGIVKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLP---VPCDPVTGFCTCRPGATGRKCDGCK 1561 HSP 11 Score: 58.151 bits (139), Expect = 2.019e-6 Identity = 48/148 (32.43%), Postives = 66/148 (44.59%), Query Frame = 2
Query: 3218 CVPCNC-GRGSLNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTG----CKKCP-PCYNGQ-----VCNNEGI-----CVCPPNTIGNLCNKCSED--SWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRC 3604
CVPC C G SL CD ET C+ C + A C +C +GY+G+ V G C++C P + C EG+ CP G C +C+ N C++C C GS C+ ++G C C+ G KCD C
Sbjct: 1417 CVPCQCNGHSSL---CDPETSICQNCQHHTAGDFCERCALGYYGI-VKGLPNDCQQCACPLISSSNNFSPSCVAEGLDDYRCTACPRGYEGQYCERCAPGYTGSPGNPGGSCQECECDPYGSLPVPCDPVTGFCTCRPGATGRKCDGC 1560
BLAST of Laminin subunit alpha-2 vs. UniProt/SwissProt
Match: sp|A0JP86|LAMC1_XENTR (Laminin subunit gamma-1 OS=Xenopus tropicalis OX=8364 GN=lamc1 PE=2 SV=1) HSP 1 Score: 451.44 bits (1160), Expect = 7.733e-129 Identity = 314/1050 (29.90%), Postives = 497/1050 (47.33%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAKSDDDNL---WWQSPSMAQGLKY-QNVNVTIDLKQVYQIVYILLKMGDSPRPGNWIL-ERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKI------SDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR-----LFHSVSIEDRRLQRQVFIYIKPTE--WKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQG---QCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP----KKKISCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS----CKKCDCDSLGST--SENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--PNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWN 3205
NA + ++ TCGT PE +C + + C +CD+ +H E+ ++ WWQS +M G++Y +N+T+ L + + I Y+ LK S RP ++ L +R+ + ++P+ +++ + Y F R + + T + + CT +S L G + S + GR S N + + L +++A IR + T ++ + + + YYY++ + +GG+C CN HA +C + N CNCKHNT GS+CE C P +N RPW+ C CNCN +S EC ++ ++ R G GG C C +NT+G NCE+C + +YR N PC CQC+ +G++ + N G C CK G G CD C G+ C PC C+ GS C+CK +V + C+ CK G+F+LD ANP GC PC+C+G ++ CSS + S ++GW G + +L S+ +S S I E + Y+ AP +LGN SYG + ++E + T + + D++++ R + S QR +F + T+ W+ + + EF L + + I+ Y +++S L V A P +E C+CP G+ G CE C GYRR N QG C C C HS+TCD +G C +C HNTAG HCE C+ GYYGD+ + + + C+ CPCP + ++ VC C T+G C+ C +GY+G+P C+ C+C+NN + +CD +TGEC +C YNT G C C+ GFYG+ L ++ C+ C C+ G+ C+++TG+C+C + R C +C G Y L + C C+C G S + C+ +GQC C ++G +C++C +F + CK C C P + +G+C+C S + CD+CE +++N
Sbjct: 43 NATVVATNTCGTP-PEEYCVQTGVTGVTKS--------CHICDSGQFHLQHGAEYLTDYNNQAEITWWQSQTMLAGIQYPSTINLTLHLGKAFDITYVRLKFHTS-RPESFALYKRTHEDGPWIPYQYYSGSCEKTYQKFNRGF----IRTGED-----EQQALCTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPV------LQEWVTATDIRVTLNRLNTFGDEVFS-----DPKVLKSYYYAISDFAVGGRCKCNGHASEC---VRNEFEKIVCNCKHNTFGSDCEKCLPFYNDRPWRRSTADSPNECLPCNCNGRSQECYFDP----------ELYRSTGHGGHCTGCADNTDGPNCERCRENYYRQDNN-----EPCHACQCNPVGSLSTQCDNY--------GRCSCKPGVMGEKCDRCQPGFHSLTEAGCRPCACNPAGSTDECNVETGR-CSCKDNVEGFN-CERCKPGFFHLDEANPRGCTPCFCYGHSSVCSSAEGYRVSSIVSTFESGVEGWTAQQRDGSEYSL----SWVSDSSAVSVISESY---FPIYFIAPAKFLGNQGASYGQNLTFSFRVERRDT----RLSAEDLVLEGAGLRVSVPLIAQGNSYPSETTQRYIFRMHEATDYPWR-------PSVSPFEFQKMLQNLTAIKIRGSY--SERSAGFLEEVSLVTAVAGAGPSAPWVEICSCPTGYIGQFCERCAPGYRRENPS--QGPYSPCVLCTCNGHSDTCDPESGVC-DCQHNTAGPHCERCSEGYYGDSTTGSASDCQPCPCP----GGSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGYFGDPLGENGAPRPCRICECSNNIDPNAVGNCDRLTGECLKCIYNTGGFYCDRCRDGFYGNPLAQNPDLKCRACSCNPYGTVKGQSGCNQVTGQCECLPHVTERDCSACEPGFYNLLSGRGCERCDCHSLGSTSGQ--CDVRTGQCECHPGISGQQCQQCEPNHFGFGPEGCKPCDCDPEGSGSLQCKEDGRCECKSGFVGTRCDQCEENYFYN 1005 HSP 2 Score: 152.91 bits (385), Expect = 3.071e-35 Identity = 169/694 (24.35%), Postives = 273/694 (39.34%), Query Frame = 2
Query: 3083 KCLCSPHALSADCDLNNGK---CKCGSNVINRICDKCENLHYWNNR----------TDCVPCNC-GRGS---LNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVE-NSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQ---YQNDILDKFIILEDSNV---INSNLTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI----KGIPCKSCDCNQQGSQSLNCNEFGIC--------QCSPGVLGIKCDQCAPNY 5038
+C C+ HA ++C N + C C N C+KC L ++N+R +C+PCNC GR +P+ TG+ G TGC NT G C +C E+ + + C C C GS S C+ G C+CK G KCDRC G++ C+PC+C G+ +C+ TG+C CK NV G C+ C+ G F D GC CFC+G S C + +R+ S +E + G +YS ++ + + PIY F + + + +++ + V+ D + E ++ +E G+R V +I+Q Y ++ ++I +++ F L N+T++K++ Y+++ L ++ ++T G VE C C G C+ GY +E N S + C CNG+S CD +G C +C N G C+ C EG+Y + + C+PC CP +C + + E+ C C +G +GK CE C G++G+P+ PC+ C+C S +++ N G C +C G CD+C +
Sbjct: 268 RCKCNGHA--SECVRNEFEKIVCNCKHNTFGSDCEKC--LPFYNDRPWRRSTADSPNECLPCNCNGRSQECYFDPELYRSTGH--------------------GGHCTGCAD--------------------NTDGPNCERCRENYYRQDNNEPCHACQCNPVGSLSTQCDNY-GRCSCKPGVMGEKCDRCQPGFHSLTEAGCRPCACNPAGSTD----ECNVETGRCSCKDNVEGFNCERCKPGFFHLDEANPRGCTPCFCYGHSSVCSSAEGYRVSSIVSTFESGVEGWTAQQRDGSEYSLSWVSDSSA--VSVISESYFPIYFIAPAKFLGNQGASYGQNLTFSFRVERRDTRLSAE-----DLVLEGA----GLRVSVP----------------------LIAQGNSYPSETTQRYIFRMHEATDYPWRPSVSPFEFQKMLQNLTAIKIRGSYSERSAGFLEEVSLVT-----AVAGAGPSAPWVEICSCPTGYIGQFCERCAPGYRRE--NPSQGPYSPCVL-----------CTCNGHSDTCDPESGVC-DCQHNTAGPHCERCSEGYYGDSTTGSASDCQPCPCPG-----GSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGYFGDPLGENGAPRPCRICEC----SNNIDPNAVGNCDRLTGECLKCIYNTGGFYCDRCRDGF 851 HSP 3 Score: 138.272 bits (347), Expect = 9.141e-31 Identity = 104/341 (30.50%), Postives = 142/341 (41.64%), Query Frame = 2
Query: 2594 GVHCDRCIEGYY-GNPKKKI--SCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDS-------------------LGSTSE--------------------------------------NCDKLTGKC-DCRTNFVNRQCDSCTSGLYL------KDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY---PNCKKCLC-SPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNC---GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYF-GLDVTGCKKCPPCY 3382
G C+RC GY NP + C C CN + ++CDP +G C C +NT G C+ C G+YGD+ T S C+ C C LG+T + NCD+LTG+C C N CD C G Y D KC C+C G + + CN +GQC CL +V C C G++N C++C C S + S CD+ G+C+C + + C +CE H+ C PC+C G GSL K E G C+C C +C+ YF GC++CP CY
Sbjct: 681 GQFCERCAPGYRRENPSQGPYSPCVLCTCNGHSDTCDPESGVCDCQHNTAGPHCERCSEGYYGDSTTGSASDCQPCPCPGGSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGYFGDPLGENGAPRPCRICECSNNIDPNAVGNCDRLTGECLKCIYNTGGFYCDRCRDGFYGNPLAQNPDLKCRACSCNPYGTVKGQSGCNQVTGQCECLPHVTERDCSACEPGFYNLLSGRGCERCDCHSLGSTSGQCDVRTGQCECHPGISGQQCQQCEPNHFGFGPEGCKPCDCDPEGSGSLQCK---EDGRCECKSGFVGTRCDQCEENYFYNRSGPGCQECPACY 1018 HSP 4 Score: 111.694 bits (278), Expect = 1.100e-22 Identity = 113/485 (23.30%), Postives = 200/485 (41.24%), Query Frame = 2
Query: 4613 KKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYSGCT-------------KDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSEL-------LNSIN-----KFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKI--LKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKI-FRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQL 5953
+ C+CN Y V C+ VTG C+ C+ + C C GFY N + CE C C S Q R +C+C G SG+ C+ C+ +G +G CK CDC+ +GS SL C E G C+C G +G +CDQC NY + + C +CY +DLL + T E + + ++ + +N + + Q S ++ L IN + +L+N I++ K +A+++ E + LE + ++ +I+ E G N + A + + +E +I AA D N+ ++ Q + S+ L I L K A +I ++ + + + +E + I + + I + + D ++K+ ++LD+ LI +R+ EDL +++ + K+L+ ++ QL
Sbjct: 865 RACSCNPYGTVKGQSGCNQVTGQCE-CLPHVTERDCSACEPGFY-NLLSGRGCERCDCHSLGSTSGQCDVRTG-----QCECHPGISGQQCQQCEPNHFGFGPEG--CKPCDCDPEGSGSLQCKEDGRCECKSGFVGTRCDQCEENYFYNRSGPGCQECPACYRLVKDKVNEQRGKLQELEDLLKNLSTGEENITDQAFEERLREAEKAVNDLLLDAQSSKDVDQGMLDRLAEINTTLSFQLERLQN-IRDMIRDTDKQAQEARDRVENTEFIIDSARVQLEKAKMAIANV--SITPPESTGDPNNMTLLAEEARKLAERHMQEARDIEKAAKEANDT------ANEALRLLQKTLASENQTALDIEELNRKYAQAKDIARELEKQASKVHAEAEEAGNRALQIYANLTSVPSIDTTALQNEADKIQKEAEELDS----------LIDRKLRDYEDLREDMRGREMEVKNLLDKGKTEQQTADQL 1321 HSP 5 Score: 106.301 bits (264), Expect = 4.355e-21 Identity = 106/376 (28.19%), Postives = 147/376 (39.10%), Query Frame = 2
Query: 2711 CRCSYNTMGTECQLCKHGFYGDALTKS----CKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKD-----KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNC-KCAQNVARRDCSKCDVGYFGLDVT-----GCKKCPPCYNGQV-----CNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTF 3766
C C +G C+ C G+ + ++ C C C+ S+ CD +G CDC+ N C+ C+ G Y C PC C G S T C C G RCE C +GYF P G N R C CE +N D P G CD TG C KC N C +C G++G + C+ C G V CN G C C P+ C+ C ++ GC++C+C+ GS S C+ +G C C G +C +C ++GF C+PC C G+ S +C K G+C+CK +GT+C C + F
Sbjct: 673 CSCPTGYIGQFCERCAPGYRRENPSQGPYSPCVLCTCNG---HSDTCDPESGVCDCQHNTAGPHCERCSEGYYGDSTTGSASDCQPCPCP--GGSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGYFGDP------------------------LGENGAPRPCRICE----CSNNID--PNAVG------NCDRLTGECLKCIYNTGGFYCDRCRDGFYGNPLAQNPDLKCRACSCNPYGTVKGQSGCNQVTGQCECLPHVTERDCSACEPGFYNLLSGRGCERCDCHSLGSTSGQCDVRTGQCECHPGISGQQCQQCEPNHFGFGPEGCKPCDCDPEGSGS---LQC-KEDGRCECKSGFVGTRCDQCEENYF 1003 HSP 6 Score: 95.5153 bits (236), Expect = 7.862e-18 Identity = 75/255 (29.41%), Postives = 108/255 (42.35%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-----CRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGR---ISSEKFCNTT-SGQCF-CLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY 3382
RCKCN + C V E C C +NT G++C+ C FY D + ++ DS +C+PCNC + + E + +T G C C +N +G CE+C E Y+ N C C C+P +LS CD N G+C C V+ CD+C+ + C PC C +C++ETG C C NV +C +C G+F LD + C PC+
Sbjct: 268 RCKCNGHASEC--VRNEFEKIVCNCKHNTFGSDCEKCLP-FYND---RPWRRSTADS------------------------------------PNECLPCNCNGRSQECYFDPELYRSTGHGGHCTGCADNTDGPNCERCRENYYRQDNNEPCHACQCNPVGSLSTQCD-NYGRCSCKPGVMGEKCDRCQPGFHSLTEAGCRPCACNPAGSTDECNVETGRCSCKDNVEGFNCERCKPGFFHLDEANPRGCTPCF 479 HSP 7 Score: 78.1814 bits (191), Expect = 1.726e-12 Identity = 77/288 (26.74%), Postives = 118/288 (40.97%), Query Frame = 2
Query: 4295 NVINSNLTRDIFMMALLNVTSLKVKLYTDKV--NPPLIKIKIITYKVMETTQAIGKRV-----------TAVEKCICNAL--TFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCY 5071
N NS + ++ + VT ++ + D+V +P ++K +Y + A+G R EK +CN TFG+ C+ + Y + + + + N L CNCNG S+ C G C C N +G C+ CRE +Y+ D N + C CQC + + C +C C+ G G+ C+ CQ GF+ G C+ C CN GS CN E G C C V G C++C P + + C C+
Sbjct: 216 NFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFSDPKVLK----SYYYAISDFAVGGRCKCNGHASECVRNEFEKIVCNCKHNTFGSDCEKCLPFYNDRPW------RRSTADSPNECL----PCNCNGRSQECYFDPELYRSTGHGGHCTGCADNTDGPNCERCRENYYRQDNN-EPCHACQCNPVG-SLSTQCDNYG-----RCSCKPGVMGEKCDRCQPGFHSLTEAG--CRPCACNPAGSTD-ECNVETGRCSCKDNVEGFNCERCKPGFFHLDEANPRGCTPCF 479
BLAST of Laminin subunit alpha-2 vs. TrEMBL
Match: G7YIN3 (Laminin alpha 1/2 (Fragment) OS=Clonorchis sinensis OX=79923 GN=CLF_108864 PE=4 SV=1) HSP 1 Score: 1343.56 bits (3476), Expect = 0.000e+0 Identity = 857/2689 (31.87%), Postives = 1321/2689 (49.13%), Query Frame = 2
Query: 158 EILSVNVDGLLMENAKITSSATCGTGGPEIFCRLVDE----IAIQRL------------INPDSH-------VLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYP-DLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPN--GECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQ----------YLTSENSYIDERFNNPTV------YYWTAPPPYLGNWITSYGTIFRYVVK-------------------------IELQSTLKGSLIDSPDVIIKCGDFRLFHSVS---------IEDRRLQRQVFIYIKPT----------EWKIGKLT---------------PDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNV-----------LYQGQCEACA---CYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSS------ENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQG-RISSEKFCNTTSGQCFCLENVNGD-RCEKCVEGYFNY-PN-CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWN-NRTDCVPCNCGRGSL-------NPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSN---ICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSC------AENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSE--KCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRR-----------FGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLD-NKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQ------NDILDKF--IILEDSNVINSNL---------TRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVM-ETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFN-------LPNSTKSGSMRTGNRILI--NLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESS--LELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQ-FQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQI-IKYTQSHIQS--DQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISIN--------VREIEDLMKNLSSNHLVSK--------------------SLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDI-------LAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYR------------------DLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHA--FAFTLERNNQ-YKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSN-----VRIGPQTNIFVGGF-PSSKLLRSLKLEG---------RLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNI-------ESVNTFKRSVRRQKRD--VQTNFDLHFTG-NGFAMIN 7405
+ L ++ D +L ENA+IT++ATCG E +CRLV+ + +RL P S+ V C CD RNE+ RHPIEF +S + +LWWQSPS+A+GLKY V +T+D KQVYQIVY+LL+MGDSPRP NWILERSLDG+ Y PW+FFAE+ Y C L++ L +T+ +P L DDE++CTTYYSQP++L SGEII++L R S ++ ++V LI+FLSARY+R RF K TL+ D M L+ S+YNRYYYS+R I++GGKCICN+HA++C++ + +G+ A C C+HNTCG+NCETCCP+FNQ+PW+P VCEECNC+ K++ C +NQTVA L+LS K G EGGGVC++C+E+T GINCE C G+YRP +V PC C+C G+ G CVSN +I ++ PGDCICK+G+ G CD C+ GY R +C+ C C + GS R YRC+PPCNCKV+VN D C+ C+ GYFNLD+ NPEGC+PCYC G+T++C +S +S L GW + F G S++ +L ++ ++ + ++ YYW+AP YLG +T+Y + +++ + ++ L + PD++++ +RL + + IE ++ + ++P + G LT P + AT + ++ L K+D+ LIK KY S+Q +IEL + L R E +P +E+C CP G G SCESC GY R +V +++G+ E C C+ HS CD G+C +C HNTAG CE CA G+YGD KS + ++C+ C CP L + T C + Y+C C T G +C+ CIEGYYG P+K I C++C C C+ TG C C +NT G C C G +GD L ++C+ C+C +GS S C G+C CR + +CD C +G D C PC C G + + C+ +GQC C G C KC GY++ P+ C +C+CS A+ CD +G+CKCG NV+ CDKC+ ++WN +C+ C+CG G++ CDM+TG C+CA +V R C++C+ GYFG+ GC+ CP C NGQ+C+ G C+CPPNT G+ C +C++ SWD+N GCK CNC GS S C+ +SG C+CK + G CD+C+ GYYG+PNC+PC C NGT+S +T C+ G C CK NV G +C C+ GTF + Y GC++CFCF ++ KC + R I +S G + I L+ R F W + RP ++ + +L L+ Y S+ + + +CL C P+Q + + G V S+ VD + G+ V +YQ + I D + I L +S+ + + + +R+ M+AL+NVTS V+L+ + P L+ +K Y+ M E ++G + ++EKC C + + G+HC+ SG+Y LP + ++R I ++KC C+G S CD VTG CQ C GN G C C EG++ + K C CQCP+ ++A CT + + +C C+ GY+G CE C G++G+P+ I C+ C+C+ GS+S NC++ G C C PG+ G +CDQC P++VV+ C+ C CT +LL++ D + + N LN +++ Q + L + K N+ + Q K ++ GEI L R + E ++ ++ + L + +++ I+ + EMP+ + L+ K +L++I + TQ + D+IN L + D+ + + + + ++ L+ + ++K + +I + L+ +K DA+ K+ + +L I +R+ D + LS L S+ + +P L V + L + S I + ++ A EAY +I +I + + A+ ++ + I + K LAE + E N +N + ++++KQ + +++ +K S+ + +V+ +K+ L+ P+ + I L + G L+ +K +N+Y LV + E IK L + LR +AR M ++ +GA A+ PI CV V Y L S F +EF+ + + ++ + I H FAFTLE + +F ++ N +EL E + + +T G+ M++ D +++ + + N +N + + Q I +GG PS L + + G RL+S + C+F ++L T GL D+ A C+ + C+ + N I D +I + V T RS R+ + +Q F F G G+A I
Sbjct: 6 DFLDMDPDSILEENAEITANATCGEHRREYYCRLVEHADGALIYKRLERERRQVMTYFQQQPRSYRDPSGQWVQCSFCDARNESLRHPIEFVRSGEPHLWWQSPSLAEGLKYHAVTITMDFKQVYQIVYVLLRMGDSPRPANWILERSLDGQNYKPWVFFAESEYDCKRLYEPLLEGPLTVTSGPRPWKLEDDEVYCTTYYSQPQALQSGEIIVTLSLDRASTISTTLGLERAVSPKLIDFLSARYVRLRFQKLQTLSGDWMAMPNQLDSSVYNRYYYSIRTIKVGGKCICNSHANRCEQRVVDGVPRAVCVCQHNTCGTNCETCCPMFNQQPWRPGRVCEECNCHGKADSCVFNQTVANLRLSQRKDGVFEGGGVCVDCREDTTGINCESCKPGYYRPVHVRPDSPNPCVRCECDTAGSTGVCVSNEREIPNRYPGDCICKEGFAGKRCDQCAEGYRRSDTDPSKCVACTCDVRGSSRGGSYRCEPPCNCKVNVNPDSYCRECQPGYFNLDAENPEGCQPCYCSGLTDKCDGISPITALVSTLNGWSI-LFPGTATDSGYLVSYRDDNVGEGESSFLLADIRVVESWLSAASLRPIDRGYYWSAPTDYLGKQLTAYRSQLTVILRFNSPTMQAFGVSYSPDTSRRISTSSLAIRDQLDAMWLSEPDIVLEGNGYRLAYILPPDYRANHLIIEIPLIESSFRVLVEPPSSVPLDRIPIDLSQGSLTSSRIAPYGGSNGMQLPGRPATIADMVAVLSKLDRFLIKVKYISDQATIELRQVKLERAERD-PDGRIPSLEECICPAGHTGTSCESCAFGYWRSQDVNVTTEPPRMLGMWRGRVEVCVPCECHGHSAHCDERTGKCTDCQHNTAGDKCELCAPGFYGDPKSGSPSACQPCECPNLANKKTGSCVANEQAPPTDLRPYLCLDCEDYTRGRYCELCIEGYYGQPEKGIPCQKCDCGRGAIGCNGTTGLCICGFNTAGPRCDACAEGTHGDPLLGQACRPCNCHEMGSVSPTCRLGDGQCTCRPGYEGVRCDRCVAGRGNVDAGCPPCQCSPLGTQQNVRPPCDQVTGQCVCKHGFGGTLDCSKCAPGYYSLGPDTCTECMCSSRAIDRVCDPEDGRCKCGENVVGNTCDKCKTGYFWNVTGPNCLACDCGIGTIIDGTVGGAVGCDMQTGQCRCAPHVTGRQCTECEPGYFGVSAKGCQPCPSCPNGQLCDQITGKCICPPNTQGDRCEECTKGSWDYNPVAGCKDCNCSEVGSVSGADTTCDLVSGKCSCKRGYTGRACDQCDAGYYGYPNCRPCECDPAGSLRPNGTEST-ITLCNPADGSCFCKANVQGERCDECKPGTFGLNIDYPLGCYTCFCFPSNQKPKCSLLTGFRSSRGREKGVEIIASDHSSTPGG--ALIQLDIGLKDRGNNDLTVGVSQFNWRFSDHRPTFLEIPEL--KGPLTRNYGSVVIELLTECLPMGDCHLNPPDQPSGLVVNGGAGVGPNTDVAGSLIVRRPYRVDAKMVAFGGR-VQLEYQPSDDAPSAIQDGYRQIWLRESDWVLTRVVDVDTRVRPSRNELMLALMNVTSFSVRLFMPEAKPKLVTVKYRIYQAMSEMKTSVGTPIRSIEKCECPSTSSGDHCEIASSGFYFPPLIPKPGTSLLPPDPSAPAIRDPQNIQWTGGVEKCRCHGMSSHCDPVTGVCQACTGNTVGPDCGQCAEGYFGDPKKGQTCVKCQCPTEQTDYAITCTPFQDRPYVAHQCVCKPGYTGPRCERCAPGYFGDPLSMIACQPCECDVSGSKSANCDQRTGACDCWPGIQGRRCDQCEPDHVVDAGRCRDCRGPCTGELLIKADEVKSQIDN-------------LNMTSLAYQGLARLGQQVEGARKRFNKERATKEQELVKHAHSVAKQTGEIRALADRVMATRNAIEVARCSSTENVTQLQK--TAQRLEQRVREWIQRIEELSVEMPDERVLQRWLEQARKVNTDLSKINLDSTQEWLDKLMDEINELISSAEQLSKLDKYSKTTADLERLTAYQSQQINLH----DAQIKLMYEISNSTARGNVRLKDAQEKSDALKKAATEAAELNVIQAFQESLEKLRKQIDQIATLSPEELQSRLDEAKAALKYITGIDLADLDGIYVPPNLLSGVVEPLERESERILSTLRYSTKINQTIVALEAYESIVNNIKKAEEATIKAEEALQESTGPESWQERREKIQLIGKKLAE-DVEAQEATVNSRN--ETLKEVIKQVDSSKMELDEVQKTADKTLSDAKSAIKNVQSVKQLLASTD------------PLTRNISALVE---QQGSLMSRLQNRLKSAENRYAKLQGTAQSAVDQANGTQTSLVKAITEAEESIKQLDSKLTHLRRLADEARSMLDVNYGALAQDPVPISLDRECVYSLVPYSLTKSRIFDIEFWFRLHASSSETDGVMMVGRRAFIGHTQMFAFTLESAGKTLRFSWNIPNG--KLELD-SIKEETWYQVRVTSMWGETKMMLLERPDEGSQKPQYREVATNASANYPESALMLDRQMEIRIGGANPSQSRLLNPEAWGDNGAEVFWTRLLSLDSTQF-------CIFNLRLGATAIGLVDLAAASSGCQQP--------SRMVCQIS-NYRPIVRDGHIWRTQGKMDDVTTTPRSPRKSPPNFVLQRMFFYDFAGAGGYARIQ 2630
BLAST of Laminin subunit alpha-2 vs. TrEMBL
Match: A0A074ZCC3 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_10681 PE=4 SV=1) HSP 1 Score: 1323.53 bits (3424), Expect = 0.000e+0 Identity = 851/2705 (31.46%), Postives = 1325/2705 (48.98%), Query Frame = 2
Query: 110 FILLIVHKAHSQDDEP-----EILSVNVDGLLMENAKITSSATCGTGGPEIFCRLVDE----IAIQRL------------INPDSH-------VLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPD-LKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYER--YPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQC---SSLSAKNLK-------ISDLKGWKLTTFTGEKIALPKFTSFQ----------YLTSENSYIDERFNNPTV------YYWTAPPPYLGNWITSYGTIFRYVVK-------------------------IELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKI----------------------------------GKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNV-----------LYQGQ---CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSS------ENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQG-RISSEKFCNTTSGQCFCLENVNGD-RCEKCVEGYFNY-PN-CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWN-NRTDCVPCNCGRGSL-------NPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSN---ICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSC------AENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSE--KCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRR-----------FGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLD-NKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQ------NDILDKF--IILEDSNVINSNL---------TRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVM-ETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFN-------LPNSTKSGSMRTGNRILI--NLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESS--LELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLP--STLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFT----DINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQIN----NLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQ------RDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVS-KSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDI-------LAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYR------------------DLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHA--FAFTLERNNQ-YKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSN-----VRIGPQTNIFVGGF-PSSKLLRSLKLEG---------RLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNI-------ESVNTFKRSVRRQKRD--VQTNFDLHFTG-NGFAMIN 7405
+ LI+ A+ Q P + L ++ D +L ENA+IT++ATCG E +CRLV+ + +RL P S+ V C CD RNE+ RHPIEF +S + +LWWQSPS+A+GLKY VYQIVY+LL+MGDSPRP NWILERSLDG+ Y PW+FFAE+ Y C L++ L +T+ +P L DDE++CTTYYSQP++L SGEII++L R S ++ ++V LI+FLSARY+R RF K TL+ D M L+ S+YNRYYYS+R I++GGKCICN+HA++C++ + +G+ A C C+HNTCG+NCETCCP+FNQ+PW+P VCEECNC+ K++ C +NQTVA L+LS K G EGGGVC++C+E+T GINCE C G+YRP +V +PC C+C G+ G CVSN +I ++ PGDCICK+G+ G CD C+ GY R +C+PC C + GS R YRC+PPCNCKV+VN D C+ C+ GYFNLD+ NPEGC+PCYC G+T++C S ++A L+ +S L GW + F G S++ +L ++ ++ + ++ YYW+AP YLG +T+Y + +++ + ++ L + PD++++ +RL + + + R + I + + +++ G P + AT + ++ L K+D+ LIK KY S+Q +IEL + L R E +P +E+C CP G G SCESC GY R +V +++GQ C C C+ HS CD G+C +C HNTAG CE CA G+YGD KS + +C+ C CP L + T+ C + Y+C C T G +C+ CIEGYYG P+K I C++C C C+ TG C C +NT G C C G +GD L ++C+ C+C +GS S C G+C CR + +CD C +G D C PC C G + + C+ +GQC C G C KC GY++ P+ C +C+CS A+ CD +G+CKCG NV+ CDKC+ ++WN +C+ C+CG G++ CDM+TG C+CA +V R C++C+ GYFG+ GC+ CP C NGQ+C+ G C+CPPNT G+ C +C++ SWD+N GCK CNC GS S C+ +SG C+CK + G CD+C+ GYYG+PNC+PC C NGT+S +T C+ G C CK NV G +C C+ GTF + Y GC++CFCF ++ KC + R I +S G + I L+ R F W + RP ++ + +L L+ Y S+ + + +CL C P++ F+ + G V S+ + VD + G+ V +YQ + I D + I L +S+ + + + +R+ M+AL+NVTS V+L+ + P L+ +K Y+ M E ++G + ++EKC C + + G+HC+ SG+Y LP + ++R I ++KC C+G S CD VTG CQ C GN G C C EG++ + K C CQCP+ ++A CT + + +C C+ GY+G CE C G++G+P+ I C+ C+C+ GS+S NC++ G C C PG+ G +CDQC P++VV+ C+ C CT +LL++ D + + N +L LR + S E + K + + + + + + D IE + + + L+ T + E I E+L + + +LE + + ++ NI L S + + K +DE+N++I + + D+ + +L+ L +++ + + +S + + E+ L+K + + +++L K +++D I+ +E + + E + +K ++ L + +P L V + L + S I + ++ A EAY +I ++ + + A+ ++ + I + K LAE + V + KE ++KQ + +++ +K S+ + +V+ +K+ L+ P+ + I L + G L+ +K +N+Y LV + E IK L + LR +AR M ++ +GA A+ PI CV V Y L S F +EF+ + + ++ + I H FAFTLE + +F ++ N +EL E + + +T G+ M++ D +++ + + N +N + + Q I +GG PS L + + G RL+S + C+F +++ T GL D+ A C+ + C+ + N I D +I ++V RS RR + +Q F F G G+A I
Sbjct: 10 LLSLILFTAYGQGYLPYTYDSDFLDMDPDSILEENAEITANATCGEHRREYYCRLVEHADGALIYKRLERDRRQVMTYFQQQPRSYRDPSGQWVQCSFCDARNESLRHPIEFVRSGEPHLWWQSPSLAEGLKYH---------AVYQIVYVLLRMGDSPRPANWILERSLDGQNYKPWVFFAESEYDCKRLYEPLLEGALTVTSGPRPWKLEDDEVYCTTYYSQPQALQSGEIIVTLSLDRASTISTTLGLERAVSPKLIDFLSARYVRLRFQKLQTLSGDWMAMPNQLDSSVYNRYYYSIRTIKVGGKCICNSHANRCEQRVVDGVPRAVCVCQHNTCGTNCETCCPMFNQQPWRPGRVCEECNCHGKADSCVFNQTVANLRLSQRKDGVFEGGGVCVDCREDTTGINCESCKPGYYRPLHVRPDSPHPCVKCECDTAGSTGVCVSNEREIPNRYPGDCICKEGFAGKRCDQCAEGYRRSDMDPSKCVPCTCDVRGSSRGGSYRCEPPCNCKVNVNPDSYCRECQPGYFNLDAENPEGCQPCYCSGLTDKCDGISPITALTLEQNGLLGAVSTLNGWSI-LFPGTSTDSGYLVSYRDDNGGEGESSFLLADVRIVESWLSAASLRPIDRGYYWSAPTDYLGKQLTAYRSQLTVILRFNSPTMQAFGVSYSSDTSRRISTSSLAIRDQLDAMWLSEPDIVLEGNGYRLAYMLPPDYRANHLIIEIPLSESSFRVLVEPPSSVPLDRIPIDLSQGSLASSRIAPYGGSNGIQLPGRPATIADMVAVLSKLDRFLIKVKYISDQATIELRQVKLERAERD-PDGRIPSLEECICPVGHTGTSCESCAFGYWRSQDVNVTTEQPTILGMWRGQVEKCVPCECHGHSAHCDERTGKCTDCQHNTAGDKCELCAPGFYGDPKSGSPTACQPCECPNLANKKTDSCVANEQSPPTDLRPYLCLDCEDFTRGRYCELCIEGYYGQPEKGIPCRKCDCGRGAVGCNGTTGLCICGFNTAGPRCDACAEGTHGDPLLGQACRPCNCHEMGSVSPTCRLGDGQCTCRPGYEGVRCDRCVAGRGNVDAGCPPCQCSPLGTQQNVRPPCDQVTGQCVCKHGFGGTLDCSKCAPGYYSLGPDTCTECMCSSRAIDRVCDPEDGRCKCGENVVGITCDKCKAGYFWNVTGPNCLACDCGIGTIVDGTVGGAVGCDMQTGQCRCAPHVTGRQCTECEPGYFGVSAKGCQPCPSCPNGQLCDQITGKCICPPNTQGDRCEECTKGSWDYNPVTGCKDCNCSEVGSVSGADTTCDLVSGKCSCKRGYTGRACDQCDAGYYGYPNCRPCECDPAGSLRPNGTEST-ITLCNPTDGSCFCKANVQGDRCDECKPGTFGLNIDYPLGCYTCFCFPSNQKPKCSLLTGFRSSRGREKGVEIIASDHSSTPGG--ALIQLDIGLKDRGHNDLTVGVSQFNWRFSDHRPTFLEIPEL--KGPLTRNYGSVVIELLTECLPMGDCHLNPPDEPSGFVVNGGAGVGPNTDVAGSLIVRHPYRVDAKMVAFGGR-VQLEYQPSDDAPSAIQDGYRQIWLRESDWVLTRVVDVDTRVRPSRNELMLALMNVTSFSVRLFMPEARPKLVTVKYRIYQAMSEMKTSVGTPIRSIEKCECPSTSSGDHCEIASSGFYFPPLIPKPGTSLLPPDPSAPAIRDSQNIQWTGGVEKCRCHGMSSHCDPVTGVCQACTGNTVGPDCGQCAEGYFGDPKKGQTCVKCQCPTEQTDYAITCTPFQDRPYVAHQCVCKPGYTGPRCERCAPGYFGDPLSMIACQPCECDLSGSKSANCDQRTGACDCWPGIQGRRCDQCEPDHVVDAGRCRDCRGPCTGELLIKADEVKAQIDNLNMTSLAYQGLARLRQQAEGARKRFSKERATKEQELVKHAHSVAKQTGEIRALADRVMATRNAIEVARCSSTENVTQLQKTAQRFEHRVREWIQRIEELSVEMPDERVLQRWLEQARKVNTDLSNINL-DSTQEWLDKLMDEINEVISSAEQLSKLDKYSKTTADLERLTAYQSQQVKLHDAQIKLMYEISNSTARGNVRLNAAQEKSDALKKAATEAAELNVIQAFQESLEKLQEQIDQIATIPPEE---LQSRLDEAKAALKYITGIDLADLDGIYVPSNLLSGVVEPLERESERILSTLRYSTKINQTIVALEAYESIANNMKKAEEATIKAEEALQESTGPESWQERREKIQLIGKKLAEAMEAQEATVNSRNETLKE---VMKQVDSSKMELDEVQKTADKTLSDAKSAIKNVQSVKQLLASTD------------PLTKNISALVE---QQGSLMNRLQSRLKSAENRYAKLQGTAQSAVDQANGTQTSLVKAITEAEESIKQLDSKLTHLRRLADEARSMLDVNYGALAQDPVPISLDRDCVYSLVPYSLTKSRIFDIEFWFRLHGSSSETDGVMMVGRRAFIGHTQMFAFTLESAGKTLRFSWNIPNG--KLELD-SIREEIWYQVRVTSMWGETKMMLLERPDEGSQKPQYREVATNASANYPESALMLDRQMEIRIGGANPSQSRLLNPEAWGDNGAEVFWTRLLSLDSTQF-------CIFNLRIGATAVGLVDLAAASSGCQQP--------SRMVCQIS-NYRPIVRDGHIWRTQGKMDNVTATPRSPRRSPPNFVLQRMFFYDFAGAGGYARIQ 2656
BLAST of Laminin subunit alpha-2 vs. TrEMBL
Match: A0A4S2LAR7 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008536 PE=4 SV=1) HSP 1 Score: 1307.74 bits (3383), Expect = 0.000e+0 Identity = 805/2454 (32.80%), Postives = 1237/2454 (50.41%), Query Frame = 2
Query: 158 EILSVNVDGLLMENAKITSSATCGTGGPEIFCRLV---DEIAIQRLIN---------------PDSH-------VLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPD-LKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYER--YPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQC---SSLSAKNLK-------ISDLKGWKLTTFTGEKIALPKFTSFQ----------YLTSENSYIDERFNNPTV------YYWTAPPPYLGNWITSYGTIFRYVVK-------------------------IELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKI-------------------GKLT---------------PDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNV-----------LYQGQCEACA---CYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELC------SSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQG-RISSEKFCNTTSGQCFCLENVNGD-RCEKCVEGYFNY-PN-CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWN-NRTDCVPCNCGRGSL-------NPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSN---ICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSC------AENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSE--KCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRR-----------FGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLD-NKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQ------NDILDKF--IILEDSNVINSNL---------TRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVM-ETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFN-------LPNSTKSGSMRTGNRILI--NLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESS--LELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKI--NISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFT----DINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASV---------LKDVLKYVDELNQIIKYTQS-----HIQSDQINNLKILKNKTIMADEI-NTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVS-KSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDI-------LAVDYAINQTKKELDFNRSNMNIDSLKKLLAE--------FNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHA--FAFTLE 6802
+ L ++ D +L ENA+IT++ATCG E +CRLV DE I + I+ P S+ V C CD RNE+ RHPIEF +S + +LWWQSPS+A+GLKY V +T+D KQVYQIVY+LL+MGDSPRP NWILERSLDG+ Y PW+FFAE+ Y C L++ L +T+ +P L DDE++CTTYYSQP++L SGEII++L R S ++ ++V LI+FLSARY+R RF K TL+ D M L+ S+YNRYYYS+R I++GGKCICN+HA++C++ + G+ A C C+HNTCG+NCETCCP+FNQ+PW+P VCEECNC+ K++ C +NQTVA L+LS K G EGGGVC++C+E+T GINCE C G+YRP +V PC C+C G+ G CVSN +I ++ PGDCICK+G+ G CD C+ GY R +C+ C C + GS R YRC+PPCNCKV+VN D C+ C+ GYFNLD+ NPEGC+PCYC G+T++C S ++A L+ +S L GW + F G S++ +L ++ ++ + ++ YYW+AP YLG +T+Y + +++ + ++ L + PD++++ +RL + + + R + I + + +++ G LT P + AT + ++ L K+D+ LIK KY S+Q +IEL + L R E +P +E+C CP G G SCESC GY R +V +++G+ E C C+ HS CD G+C +C HNTAG CE CA G+YGD KS + ++C+ C CP L + T+ C S Y+C C T G +C+ CIEGYYG P K I C++C C C+ TG C C +NT G C C G +GD L ++C+ C+C +GS S C G+C CR + +CD C +G D C PC C G + + C+ +GQC C G C KC GY++ P+ C +C+CS A+ CD +G+CKCG NV+ CDKC+ ++WN +C+ C+CG G++ CDM+TG C+CA +VA R C++C+ GYFG+ GC+ CP C NGQ+C+ G C+CPPNT G+ C +C++ SWD+N GCK CNC GS S C+ SG C+CK + G CD+C+ GYYG+PNC+PC C NGT+S +T C+ G C CK NV G +C C+ GTF + Y GC++CFCF S+ KC + R I +S G + I L+ R F W + RP ++ + +L L+ Y S+ + + +CL C P+Q F+ + G V S+ VD + G+ V +YQ + I D + I L +S+ + + + +R+ M+AL+NVTS V+L+ + P L+ +K Y+ M E ++G + ++EKC C + + G+HC+ SG+Y LP + ++R I ++KC C+G S CD VTG CQ C GN G C C EG++ + K C CQCP+ ++A CT + + +C C+ GY+G CE C G++G+P+ I C+ C+C+ GS+S NC++ G C C PG+ G +CDQC P++VV+ C+ C CT +LL++ D + + N +L RL + + + E + K + + + + + + D IE + + + L+ T + E I E+L + + +LE + + ++ I L ++ +++V+ ++L+++ KY+++ + + Q +K+ + + EI N+ + + + ++K L ++ + F +++L K +++D I+ +E + + E +D +K ++ L + +P L V + L + S + ++ A EAY +I ++ + + A+ ++ + I + K LAE NS + E +K +D +L + K K+ + + S + + L RN+S + +Q ++ +N+ L + + + + + LV E IK L + LR +AR M ++ +GA A+ PI CV V Y L S F +EF+ + + ++ + I H FAFTLE
Sbjct: 31 DFLDMDPDSILEENAEITANATCGEQRREYYCRLVEHADEALIYKRISLEREKRQVMTYFQQQPRSYRDPSGQWVQCSFCDARNESLRHPIEFVRSGEPHLWWQSPSLAEGLKYHAVTITMDFKQVYQIVYVLLRMGDSPRPANWILERSLDGQNYKPWVFFAESEYDCKRLYEPLLEGPLTITSGPRPWKLEDDEVYCTTYYSQPQALQSGEIIVTLSLDRASTISTTLGLERAVSPKLIDFLSARYVRLRFQKLQTLSGDWMAMPNQLDSSVYNRYYYSIRTIKVGGKCICNSHANRCEQRVVEGVPRAVCVCQHNTCGTNCETCCPMFNQQPWRPGRVCEECNCHGKADSCVFNQTVANLRLSQRKDGVFEGGGVCVDCREDTTGINCESCKPGYYRPVHVRPDSPNPCVRCECDTAGSTGVCVSNEREIPNRYPGDCICKEGFAGKRCDQCAEGYRRSDMDPSKCVACTCDVRGSSRGGSYRCEPPCNCKVNVNPDSYCRECQPGYFNLDAENPEGCQPCYCSGLTDKCDGISPITALTLEQNGLLGAVSTLNGWSIL-FPGTATDSGYLVSYRDDNVGEGESTFLLADVRVVESWLSAASLRPIDRGYYWSAPTDYLGKQLTAYRSQLTVILRFNSPTMQAFGVSYSSDTSRRISTSSLAIRDQLDAMWLSEPDIVLEGNGYRLAYMLPPDYRASHLIIEIPLTESSFRVLVEPPSSVPLDRIPIDLSQGSLTSSRIAPYGGSNGMQLPGRPATIADMVAVLSKLDRFLIKVKYISDQATIELRQVKLERAERD-PDGRIPSLEECICPVGHTGTSCESCAFGYWRSQDVNVTTEQPTMLGMWRGRVEVCVPCECHGHSAHCDERTGKCTDCQHNTAGDKCELCAPGFYGDPKSGSPSACQPCECPNLANKKTDSCVANEQASPADLRPYLCLDCEDYTRGRYCELCIEGYYGQPDKGIPCRKCDCGRGAIGCNGTTGLCICGFNTAGPRCDACAEGTHGDPLLGQACRPCNCHEMGSVSPTCRLGDGQCTCRPGYEGVRCDRCVAGRGNVDAGCPPCQCSPLGTQQNIRPPCDQVTGQCVCKHGFGGTLDCSKCGPGYYSLGPDRCTECMCSSRAIDRVCDPEDGRCKCGENVVGITCDKCKAGYFWNVTGPNCLACDCGIGTIIDGTVGGAVGCDMQTGQCRCAPHVAGRQCTECEPGYFGVSAKGCQPCPSCPNGQLCDQITGKCICPPNTQGDRCEECTKGSWDYNPVTGCKDCNCSEVGSVSGADTTCDLGSGKCSCKRGYTGRACDQCDAGYYGYPNCRPCECDPAGSLRPNGTEST-ITLCNPTDGSCFCKTNVQGDRCDECKPGTFGLNIDYPLGCYTCFCFPSSQKPKCSLLTGFRSSRGREKSVEIIASDHSSTPGG--ALIQLDIGLKDRGNNDLTVGVSQFNWRFSDHRPTFLEIPEL--KGPLTRNYGSVVIELLTECLPMGDCHLNPPDQPSGFVVNGGAGVGPNTDVAGSLIVRRPYRVDAKMVAFGGR-VQLEYQPSDDAPSAIQDGYRQIWLRESDWVLTRVVDVDTRVRPSRNELMLALMNVTSFSVRLFMPEARPKLVTVKYRIYQAMSEMKTSVGTPIRSIEKCECPSTSSGDHCEIASSGFYFPPLIPKPGTSLLPPDPSAPAIRDPQNIQWAGGVEKCRCHGMSSHCDPVTGVCQACTGNTVGPDCGQCAEGYFGDPKKGQTCVKCQCPTEQTDYAITCTPFQDRPYVAHQCVCKPGYTGPRCERCAPGYFGDPLSMIACQPCECDISGSKSANCDQRTGACDCWPGIQGRRCDQCEPDHVVDAGRCRDCRGPCTGELLIKADEVKTQIDNLNMTSLAYQGLARLGQQAEGARKRFNKERATKEQELVKHTHSVAKQTGEIRALADRVMATRNAIELARCSSTENVTQLQKTAQRFEHRVREWIQRIEELSVEMPDERVLQRWLEQARKVNTDLSKINLDSTQEWLGKLMDEIEEVISSAEQLSKLDKYSKTTADLERLTAYQSQQVKLHDAQIKLMYEISNSTARGNVRLKAAQEKSDALKKAATEAAELNVIQAF------QESLEKLQKQIDQIATIPPEE---LQSRLDEAKDSLKYITGIDLADLDGIYVPPNLISGVVEPLERESERVLSTLLYSTKINQTIVALEAYESIVNNMKKAEEATIKAEEALQESTGPESWQERREKIQLVGKKLAEDVEAQEATVNSRNETLKEAMKQVDSSKMELDEVQKTADKTLSDAKSAIKNVQS-VNQLLSSTDPLTRNISALVEQQGSL-------MNRLQNRLKSAENRYAKLQGTAQSAVDQANGTQTSLVKAIIEAEESIKQLDSKLTHLRRLADEARSMLDVNYGALAQDPVPISLDRECVYSLVPYSLTKSRIFDMEFWFRLHASSSETDGVMMVGRRAFIGHTQMFAFTLE 2459
BLAST of Laminin subunit alpha-2 vs. TrEMBL
Match: A0A564ZBU7 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS14019 PE=4 SV=1) HSP 1 Score: 1292.72 bits (3344), Expect = 0.000e+0 Identity = 820/2650 (30.94%), Postives = 1278/2650 (48.23%), Query Frame = 2
Query: 158 EILSVNVDGLLMENAKITSSATCGTGGPEIFCRLVDEI-----AIQRLI--------------NPDSHVL-CDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQY-PDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGE--CLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLK----------ISDLKGWKLTTFTGEK-IALP-----------KFTSFQYLTSENSYIDERFNNPTV-----YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDS----------PDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKI------------------------GKLTPD-----------------KAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEV-HLALDEMPH----IEKCNCPPGFNGLSCESCESGYRR--------------------VNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSEN-----------YVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGD-ALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRI-SSEKFCNTTSGQCFCLENVNGD-RCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWN-NRTDCVPCNCGRGSL-NPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSY--SNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSN----ELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSE--KCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYP-------------RRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLD-NKCGREIP------------------EQTEVF--MESYNGLFGMRYXXXXXXXXXXXXXXDFNEH-----SKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKI--KIITYKVMETTQAIGKRVTAVEKCICN-ALTFGNHCQ---------------NPISGYYKEYFNLPNSTKSGSMRTGNRILINLK-----KCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLP----STLDLRLNKINISIQMSSELLNS----INKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRY-NKVLEDLRSTFTDINETISGHEQL--GISNELIKNATAYLETIKNIYKEMPNITL------AASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRS-NMNIDSLKKLLAEFNSNKSEIVENIKNI-DKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDV-EILKRNLSXXXXXXXXXXXXFILPINQT-----ILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKI----KDLRKSIKSLRERLQ--------------KARVMSELIHGAAARSVEPIR--HVVRCVRHYVTYYLPTSNAFSLEFYMKPQI------------LNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVE---SIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGR-------------TEQVETSSITNNILSN----VRIGPQTNIFVGGFPSSKLLRSLKLEG------RLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEEC 7210
+ L ++ D LL E+A IT++ATCG E FCRLV+ A+ R+ + D V+ C+ CD R+ T RHPIE+A S D+NLWWQSPS+A+GL+Y V + +DLKQVYQIV++LL+MGDSPRP NWILE+S+DG+ + PW+FFAE C +++Q L++T++++P L + E++CTTY+SQP++L +GEIII L R+ + + K + LI FLSAR++R RF K TL+ D MT + L+ ++YNRYYYS+RNI+IGGKCICN+HA+ C + I +G+ A C+C+HNTCG CE CCPLFNQ+PW+P +CEECNC+ K++ C YNQTVA L LS+N+ EGGGVC+ C+E T GINCE CI G+YRP NV PC PCQC+ G+ G C+SN + +++PGDC+C++G+ G CD C+ GY + P CL CPC + GS R YRC+PPCNCK +V+ D LCK C +FNLD +PEGC+ C+C G+T C S+S + + L+ W L + + +++P + T L++E++ P YW+AP YLG +T Y + + + + D+ PDV+I+ RL +S E R+ R I + + +++ ++ PD + AT + ++ L V KL +KAKY +Q EL + + R E + L +P IE+C CPPG G+SCE CE GY R + N +++ C C C ++TCD NG C NC +NT G CE CA G++ D + C+ C CP + Q T C + S + Y C C NT G C+ CIE +YGNP C+ C C +CD +TGEC+C + T G C C G +GD + + C C C +GS S CDK TG C CR + R CD C +G + C CNC+ G + + C+ +GQC+C V G C++C +GY+N + C+ C CS ALS C G+C+CG+NVI + CD+C HYWN + +C+PC CG G+L N +CD+ TG C+CA V R C +C G++G GC++CP C NG+VC+ G C+CPPNTIG+ C CS +S+D+N +GCK CNC GS + C+ ++G CACK+ F G CD C GYYG+PNC+PC C+ GT N + CD ITG C CK NV G KC C G+F Y GC++CFCF S+ +C + R V + + N G +HL + +F W + P Y+ + + H S Y SIF+ + DCL CG+E P + ++F M + NG Y + + + D + WV+++ + S + +R ++ L N+T+ V+L T + I + +I T K TQ +G +T +E CICN T G+HC+ P Y+ + F++ +++G++ G + ++ +C CNG + CDS TG C NC GN G C++C EG+ + CE C+CPS + NFA+ C I + C+C GY+G+ C+ C G+YG+P+ IPCK C+CN GSQ NCN + G C C PGV G +CD+C P +VV+ C C CT +LL R ++E + L L R +K+ + + ++LN+ I + K+ +E+ S+ + + E L N+ D+ S NE I L G +E++ + +T + I +E+ + L A +L+D + ++L IIK T + +I+ LK + + D T E L M + + I+ I++ + + N + ++L+ + + D ++ + + D+++N + + + + PD + K ++ K L D + + +EAY I I +++ + ++ + ++ S++ LA+ + E ++ + D+ +++ L N + + F D+ +L+R L ++ + + + + T L + T N + D ++K +K ++ Q+ ++ ++ N+ K L +I + ER++ AR + +LI+G+ + P+R CV + Y+ S F LEF+ P + + S++L G + FA T+E N +F + + +E+ G + N + +T G+ + ++ I R T++ ++ S+T +L + +R+G + ++ + + +G RLI KGCL+ +K+ G D + C
Sbjct: 31 DYLDMDPDSLLAEHAVITANATCGERKREYFCRLVEHAGYNFAALLRMKRQAFSYPQDARTFKDVDGTVIECNYCDARDPTLRHPIEYAVSGDNNLWWQSPSLAEGLQYHAVTIDVDLKQVYQIVFVLLRMGDSPRPANWILEKSIDGKDFQPWVFFAENAQQCISMYQPTVNKTLRITSDSRPHKLGNAEVYCTTYFSQPQNLQAGEIIIPLTVDREGASASYFEASKPIDPELIEFLSARFVRLRFQKLQTLSGDWMTLRDQLDPTVYNRYYYSIRNIKIGGKCICNSHANTCGRQIIDGVPRAVCDCQHNTCGYTCEKCCPLFNQQPWRPGGMCEECNCHGKADSCVYNQTVANLLLSLNRYNVREGGGVCVGCREQTTGINCEDCIPGYYRPLNVGPDAKNPCVPCQCNIYGSTGVCISNDALMPEKQPGDCVCREGFAGRLCDRCAEGYYQSPIDPRGCLKCPCDVAGSHRGQGYRCQPPCNCKANVDPDSLCKACLPKHFNLDDEDPEGCQECFCMGLTKDCQSVSVATARRLEQEGKLGLVDTLEEWSLVVPSSPRSLSVPPTSAGASADEIETTRMLRLSAEDAEF--ALGPPAAAPGRSIYWSAPAKYLGKQLTGYRGYLEVITRFTSPAIRTATAYDNYTASRVWITEPDVVIEGHGIRLGYSPYPETRQTHRVQRIRLHESNFRVLVEAGNGSLDRIPIHQTQATGNGRQQMYPDPTYGQDGSYRLDYTRVGRPATIVDIMTVLSNVTKLYVKAKYTDDQTFTELRRVAMERAERKNTTLGGLPEGVRGIEECRCPPGHRGISCEECEPGYWRDPSIAPAGDGQSGEIAAFLGLVNPIFRRACVPCDCNGRADTCDPNNGHCLNCKYNTFGPKCEQCAPGFHIDPSKFGSDICQPCECPSVLNQKTNSCVALSPGDLRTFGISAEKPYACLDCEDNTRGQFCESCIEMFYGNPLLGQPCRHCDCGPMAINCDSITGECQCGHYTTGPRCTECAPGSHGDPSRGEPCTPCGCHPVGSQSIECDKDTGLCLCRLFYEGRHCDRCVAGRGNVEAGCPTCNCDPLGSVPQANASCDPVTGQCYCKPGVGGTLECDRCADGYYNLGSNGCQLCECSNRALSTACHPTTGQCECGNNVIGQKCDRCLRGHYWNGSEPNCLPCECGPGALVNNECDLYTGQCRCALYVTGRRCDQCQTGFWGPSPRGCQRCPQCPNGRVCDQLTGKCICPPNTIGDQCETCSPNSYDYNPALGCKDCNCSSIGSVDPTGDCDLLTGQCACKSGFAGRACDECAPGYYGYPNCKPCMCSLEGTLRNFSNGDQITCDPITGACLCKPNVEGEKCDKCIPGSFGLSADYPLGCYACFCFPTSQPAQCEQLKGFRSVPEAQRRLQLVGTDNQYMPGGPIVDLHLTSEVSTGSSELPDLTVGQNQFKWRFSKSMPTYLILPDVKGPHTRS--YGSIFVEIHADCLPTGNCGQEYPSTALQTRGRPSADRTVLEDPQKIFARMTALNGQLEFEYQPENLPTQTAIPGGDLAAQYNTIWMREADWVLTRIR------------SQHLRVRPSRAALVLGLTNITTFSVRLATPGIPLKAISVNYRIRTAKSEALTQ-LGTPITTIETCICNDTSTKGDHCELADELNYFPPITIKPGPDGTYFDDLFDITKPSENGTIPAGGKPSFEIEGGSVLRCECNGMASECDSETGFCMNCEGNTAGAHCEVCAEGYMGDPTQGIPCERCRCPSENANFARTCHTIGQTGSYICECLPGYAGQLCDRCDTGYYGDPVNMIPCKKCECNPAGSQHQNCNMKTGQCVCLPGVTGQQCDECPPGHVVDGGRCIDCRGECTGELLERAAEVSEKIDRIDLTELAHQGLGDLQNRTDKLLDRYESAGKILNTERDLIERASKITSELTRISLNMMTSLPSSVKAIDEASCLAANATNQFSRDIVSLERKFNEWIQSMRNLRPGQVDEIL--IQHWHQTARLIRQEIARLDLGLTSEWANKMLEDSRRLEEQLASIIKRTSTADYGSKIDRLKYYQK---LQDSHLTTQLEQLYLMGNQTQRGLDEIVSIHDMIDTTSG--GALDTLWVNQSRFEEELNVLKSNASDYQEPTMTEISRLSDILENTQTFSEIPDT-VPPDVISKAHSLEMAKDRILA---ALDRPGVDQTLEVREAYKKIADTIWEARNITDESMEAIEASGGEGVSASSIEASLADIMKRHDQATERVQRLSDRLNDEQLIIAANADRI------------TALKKSFVDINAVLQRTLEASKLTADRVNKQLLPLVTLTRPQLDALAIETANARKRFDA--IDKEVKAIEKQFNEMEGTANMAVNQANSTQKSLLDAILATEERVETVDRKMADARRLASTARSLLDLIYGSLGKDPIPLRLNGGGDCVYTLIPYHATKSRVFDLEFWFAPALQRISGTTGSATGIRTNSVLLVGRRAFPARTSIFAVTVEPNGNLRFAWSQPEVIGGELGVEI-GPLNGSNTNRVRVTAIPGRVDLYLQEIPKDRDNLEGLPIHQATYTDETDSESLTTGLLLDNQLELRVGGAFHSVSAAGSNAPPMETWGDDGAEEIWNRLIK-------MEHAKGCLYELKISGHRLGFPDFAKADPAC 2630
BLAST of Laminin subunit alpha-2 vs. TrEMBL
Match: A0A5K4FDV5 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 1285.01 bits (3324), Expect = 0.000e+0 Identity = 832/2483 (33.51%), Postives = 1252/2483 (50.42%), Query Frame = 2
Query: 86 LKMRIFIIF--ILLIVHKAHSQDDEPEILSVNVDGLLMENAKITSSATCGTGGPEIFCRLVDE-----IAIQRLINPDSHVL------------------CDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYP-DLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYER--YPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKG----------WKL------TTFTGEKIALPKFTSFQY---LTSENSYIDERFNNPTV------YYWTAPPPYLGNWITSYGTIFRYVVKI-----------ELQSTLKGS----------------LIDSPDVIIKCGDFRLFHSVSIEDRR--------LQRQVFIYIK-----------PTEWKIG-KLTPD--------KAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRR---------VNNVLYQGQ----------------CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSE------NYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCE-IQGRISSEKFCNTTSGQCFCLENVNGD-RCEKCVEGYFNY-PN-CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR-TDCVPCNCGRGSLNPK-------CDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNI---CNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTK------SNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCF--GVSEKCHAILNHRIVENSIYEYHIFDEKNSLESG----------KDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCL---DNKCGREIPEQTEVFMESY-----NGLFGMRYXXXXXXXXXXXXXXDFN-EHSKNGKWVISQYQNDIL--DKFIILEDSNVINSNL--TRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKV-METTQAIGKRVTAVEKCICNALTFGNHCQNPISGYY---------KEYFNLPNSTKSGSMRTGNRILI-NLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRI--ESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISG--HEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKI-LKNKTIMADEINTNNKEYLNTMSTKK-----------KEHELYIILINERMKDLQKI--FRKM--------TKQRDNLRKKIQKLDAIS--KSFQDEKDLISINVREIEDLMKNLSSNHLV-----------------SKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDV----EILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLD---------------TRNLEKLIKVVDNQYRDLVNESSFVENKI-----------KDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYM 6712
L + +FIIF + I+ + E + L ++ D +L ENA IT++ATCG G E FCRLV+ RL VL C CD+R+ RHPIE+ S + NLWWQSPS+AQGL+Y V +T+D +QVYQIVY+LL+MGDSPRP NWILERS+DGE+Y PW+FFA+T Y C L++ L +T+ +P L DDE++CTT+YSQP++L SGEII++L R+S ++ E + LI+FLSAR++R RF + TL+ D M L+ S+YNRYYYS+R I++GGKC+CN+HA +C++ + +G+ A C+C+HNTCG+NCE CCPLFNQ+PW+P +CEECNCN+K++EC YNQTVA L+LS+ K G +EGGGVC+NC+E+T G+NCEKC +G+YRP NV YPC PC+C G+ G C SN +I ++ PGDCICK+GY G CD C+ GY+R C+PC C I GS R Y+C+PPCNCK++V+ D C TC+ G+FNLD NPEGC+ CYC G+T++C+ ++ I + KG WK+ T TG I + + TS QY L ++ ++ ++ T YYW+AP YLGN IT+Y +++ + + + GS + PD++I+ +RL H +S R L F I P W G +L D + AT + ++ L +D+L+IKAKY ++Q + EL+++TL R + + +P+IE+C CP G++G SCESC GY R N + C C C+ HS+ CD G C +C HNTAG C+ CA G+YGD ++ + +CK C CP LDYQMT +C ++ E YVC C NT G +C+ C E YYG+P + C+ C C C+ G C C +NT G +C C G +GD + C+ C+C GS S +C G+C+C + + +CD C G + C PC C+ I R C+ SGQC C V G C C GY+N PN C +C C+ A+ C+ G+CKCG NVI CDKC +YWNN ++C+PC+CG G+ + CDM TG CKCA +V RDC++C++GYFG+ +GCK C PC NGQVC+ G C+CPPNT G+ C+KC SWD+N IGCK CNC I+GS + C+ I+G C CK F G C+ C++GYYG+P+C+ C+C GT S+ + C+ GQC CK+NV G +C C+ GTF + Y GC+SCFCF G +C + +R V + I + G +D L +F W ++ RP ++ + +L S L+ Y S+ + V +CL D K G E + T + S GL R + + + N ++ + S+N I+ I ++ +L + I++ + TR+ M+ALLNVTS V+L+ + P L+ +K Y+ E T G + +EKC C G+HC+ SG+Y +E P S ++ TG+ I +KC C+G S CD +TG C +C GN EG C C G+ + K C CQCP+ ++A CT S L KC C++GY+G CE C G+YG+P C++CDC+ GS+S CN+ G C C PG+ G +CDQC ++VV+ TC+ C CT +LL++ D + + TL L+K+ I+ + N+ +L+NEI + Q + ++ + Y + +K E L + ++ + I+ +E I N+L + N+ K+ N + +++ ++ DE QI ++++ + Q+N+ + L N + ++IN E++ + + + EL L+N M L +I + K+ K+ D L ++ ++S +SF E + + ++E L N + + S+ + IP L H + + + S I + L+ A AY I +I + A N+ + F S ID K++ + + + I + +++ N S L E+ T D+ +I K NL + N +++ Q L P V++ D + +E + + LV+ ++ ++ + + L + +LR +AR M ++ +G+ + P++ CV V Y L S F +EF+
Sbjct: 5 LSVLLFIIFNVKVAILQRYDGYPYEADFLDMDPDSILEENAIITANATCGIGRKEYFCRLVEHADGFITYDGRLHRTPREVLTYSQEAKSYRDPSGQWVQCGYCDDRDPNLRHPIEYVLSGEANLWWQSPSLAQGLQYHAVTITMDFRQVYQIVYVLLRMGDSPRPANWILERSVDGEVYHPWVFFAKTEYDCKKLYEPLIDRPLTITSGPRPWHLGDDEVYCTTFYSQPQALQSGEIIVTLTLDRESTISTESGLESPISSKLIDFLSARFVRLRFQQLQTLSGDWMAMPNQLDSSVYNRYYYSIRTIKVGGKCVCNSHASRCEQKVIDGVPRAVCDCQHNTCGNNCEKCCPLFNQQPWRPGRICEECNCNQKADECIYNQTVANLRLSMRKDGVLEGGGVCLNCREDTTGVNCEKCKQGYYRPLNVRPDSQYPCRPCECSTFGSTGDCTSNDAEIPERYPGDCICKEGYAGRRCDECAEGYQRSNIDPQLCIPCTCDIRGSHRGPGYQCEPPCNCKINVDPDSYCNTCQKGFFNLDVNNPEGCQSCYCSGLTDRCTGVTPSTAMILEKKGLLGPVNTLANWKIIVPSLSTPDTGYTIPMTE-TSVQYDKVLFAQTRTVEAWLSSVTSTPVTRGYYWSAPEAYLGNQITAYRDTLNIILRFNSPTMLTYRQPTIPTDISGSRQFSMSMAMTDHLNYMWLHEPDIVIEGNGYRLVHMLSPSYRESHMILNIPLSESSFRVIVEPPTSIPLDRFPISWLQGDQLRFDESTLERVGRPATIADIMTVLSSIDRLMIKAKYITDQTTTELMSVTLGRA-IRDEIGGIPNIEECICPNGYSGSSCESCAIGYYRDYQKQSMIQTNQTSFTSSTALSLWRSTMMTQLPVCVLCECHGHSDICDEKTGVCIDCAHNTAGDKCDICAPGFYGDPRTGDPKACKSCECPHLDYQMTTVCLAHEEELFKEEKPYVCLDCTENTRGRYCEVCAEMYYGDPLNGVPCRPCNCGPAAIGCNATNGACICGFNTAGPQCDACAEGTHGDPTRGQPCRPCNCHPKGSISPSCRLEDGQCNCLSTYEGVRCDRCIPGRGNTEAGCPPCQCDPIGTRPEYLTMCDPVSGQCSCKPGVGGTLDCSTCQIGYYNLGPNGCTECKCTSRAIDRTCNPITGQCKCGENVIGDTCDKCLPGYYWNNTGSNCLPCSCGIGTELTRTLTQLTECDMNTGQCKCAPHVVGRDCTECELGYFGVSESGCKPCLPCPNGQVCDQITGKCICPPNTEGDRCDKCIAGSWDYNPVIGCKNCNCSIDGSVIGLEDQCDLITGQCTCKPEFSGRACNECSKGYYGYPDCRKCNCDMRGTAWGNLSISDTVVPCNPKDGQCNCKENVEGDQCDQCKPGTFGLNIDYPLGCYSCFCFPTGTPSRCSLLTGYRSVPGKVKGVEIISTDDPRYPGGPLIDLKLGLRDIDVNELTIGL-SQFSWRFSVHRPTHLEIPELKGS--LTRNYGSVIIGVLTECLPTGDCKLGIEDAQTTTTTILSTRDDLPGGLIIRRNDIVDARMTALNGYIEMEYQPSENSPTSIADGYRQIWLREQDWVLTRVSGIDTRVRPTRNELMLALLNVTSFSVRLFMPEARPKLVSVKYKIYQPRTEITTLSGIEIKTIEKCECPPTASGDHCEIASSGFYFPPIKPKPGREILP-PTSIIDPTLPTGHIIWEGGAQKCRCHGMSSNCDPITGICLDCTGNTEGPNCGNCATGYMGDPKIGQPCVKCQCPTEQTDYAITCTPSTDPSFLPHKCICKVGYAGLRCEQCAPGYYGDPTSMTACQACDCDIGGSRSETCNQDTGQCDCWPGIKGRRCDQCEEDHVVDHGTCRDCRGPCTGELLIKSDYIKLQINELNITTLAYQ---GLSKLKQQIEFNKARFIESNQ--QLENEIILRTKQLTRQFNEINQSYISVILIKQ------ETLNQSNCNLIKQINQLYNESQSIENQLYN-------WMNNLLKQEFNQPIINEE-EELKRWYDEAQQI----KNNLINIQLNHTDLWLINIELQFNKINKEINEFIQIIKQQSNKINNYLIKFNRYQELQQFLMNYEMNQLYRISNYTKLLMNELDMGIKKADTLVDTVKTTTSLSTLQSFDKESKNLDQELLKLEQLHTNQEQINRIEQLKQLIQQLSQIQLPDSRQIYIPPELITHTIEPIEQESKRIIDSLIPSDQLNRTYLAYNAYQFIIDNINLAENATNEAENA--FFNSTTTIDG-KQITWQ---------QRLNTIINKRDQITDILNNYSIIYNSHNETLNILDKELQTTENDLSTLNQIAKNNLKLTKDITNKVDSI------QQFLKDTKPLVQNASDKLQVQLGRYNELHDRLQGMENRFSQIQGTAKTLVDRANITQSYLIKSIDEAETVARQLDSKLLNLRRLADEARSMLDVNYGSLSHDPVPLQLGQDCVYTLVPYSLTKSRVFDIEFWF 2440
BLAST of Laminin subunit alpha-2 vs. Ensembl Cavefish
Match: lama1 (laminin, alpha 1 [Source:ZFIN;Acc:ZDB-GENE-060118-1]) HSP 1 Score: 651.744 bits (1680), Expect = 0.000e+0 Identity = 407/1188 (34.26%), Postives = 600/1188 (50.51%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCD--NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT----HKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKC--DKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQK---PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIE--LQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQ-RQVFIYIKPTEWKIGKLTP--------DKAATKEEFISCLVKVDKLLIKAKYYSNQKSI-ELLNITLSRGE-VHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP--RLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYL--KDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR-TDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQV------------CNNEGICVCPPNTIGNLCNKCSEDS---WDFNETIGCKKCNCYINGSYSNICNKISGI 3559
L NA+I+++ATCG PE++C+LV+ + +R+ NP C CD + N +RHPI A D N WWQSPS+ G ++ V VT+DL+Q++Q+ Y+++K +SPRPGNWILERSLDG Y PW ++A + C YN+ R P P+ D+E+ CT+YYS+ L GEI SLINGR + L+ F SAR+IR R + TL ADLMT ++ + RYYYS+++I +GG CIC HA C D V + +C C+HNTCG +C CCP ++Q W+P + CE+CNC+ K+ +C YNQTVA L++S+N G+ GGGVCINC +NT G+NCE C G+YRP + PC C C G++ C+ + + K PG C+CK+G+ G CD C+ GY +P C C CS+ GS R C CKV+V + C CK G++NL ++NPEGC C+CFG+++ C + W + T +YG+ Y V + + + + +L DV+I+ G+ R R+LQ R+VF+ + + + P ++ ++E +S L V L I+A ++ + L +++L+ + + + P +E+C CP G++G SCE C SG+ RV +L+ G C C C +H+ CD +NG C C+HNT G HC+ C GYYGD C++C CP + CS +C +C +G C+RC GYYGNP C C+CN N + CD VTGEC +C +T G C+ C+ G++GDA+T K C+ C C + GS S C+ +TG+CDCR N V +CD C G + C C C G +S + C GQC C+ V+G +C++C +G++N+ + C C C+ +C+ G+C C + C+ CE H+ ++ T C PC C GS + +CD+ G C C + A + C +C +GY C C NG C + G CVC N G C++C + W N + GC C C+ S +C ++ G+
Sbjct: 24 LASNAEISTNATCGEPLPEMYCKLVEHVPGRRIRNPQ----CRTCDANSANLKERHPITNAI-DGTNQWWQSPSIKNGRQFHWVTVTLDLRQIFQVAYVIIKAANSPRPGNWILERSLDGINYQPWQYYAISDTECLTRYNITPRLGP---------PTYKRDEEVICTSYYSRLVPLEHGEIHTSLINGRPGA--------DDLTPELLEFTSARFIRLRLQRIRTLNADLMTLSYRDPKDVDPIVTRRYYYSIKDISVGGMCICYGHAQSCPWDPVTKK----LQCVCEHNTCGESCNECCPGYHQELWQPGTISDGNTCEKCNCHNKAEDCYYNQTVADLKMSLNTHGQFVGGGVCINCTQNTAGVNCETCADGYYRPHKMSPYATEPCLECNCDMRGSVSHVCLKDDKHANPGKGLLPGQCVCKEGFAGEKCDRCAFGYRDFP--LCTRCECSLKGSHNIDPCR---ECICKVNVMGAN-CDLCKQGFYNLQASNPEGCTECFCFGVSDVCEGST-----------WNTSLLT----------------------------------------------AYGSFLNYSVAYDASVDNNVDKTLPSHFDVVIQ-GNGRSL-------RQLQSRRVFLNPHMEQHVVLEFLPHFFVDMHMERRVQRDELMSVLADVASLRIRAHLNTSVDGVLRLSSVSLAVADPISRSAAPAPDVEQCECPWGYSGTSCEYCISGFYRVGGILFGGNCLQCECNDHATECD-INGVCLGCSHNTTGPHCDQCLPGYYGDPTEGTAEDCQRCACPLTLASNNFSPTCSLQGPGEIMCDQCEQGYTGTKCERCANGYYGNPTVAGQKCSVCECNGNVDPSEPGHCDAVTGECLKCVGHTSGRHCERCEDGYFGDAVTAKDCQACGCHANGSVSSVCNIITGQCDCRANVVGDKCDRCQVGFHGINSGDGCRQCECSQSGSVS--EACE-EDGQCQCIVGVSGAKCDRCQQGFYNFTDGGCTPCDCA--HTHGNCNAETGECICPPHTRGEKCEVCEEDHWGHDAVTGCKPCECSVDGSNSSQCDLLNGQCACRPHFASQKCDRCALGY--RKFPECTACNCNTNGTREDFCDDELEVCGCEDHGQCVCKDNVGGGGCDECKTGTFGLWGQNPS-GCSPCFCF---GVSTVCEELGGL 1102 HSP 2 Score: 378.637 bits (971), Expect = 1.624e-104 Identity = 344/1271 (27.07%), Postives = 558/1271 (43.90%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYY--GNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGD---ALTKSCKKCDCD-SLGST--SENCDKLTGK----CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCE-NLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKS----NELTKCD-KITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIF---DEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINL-KKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK-NYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETV--INFKWKTL-PSTLDLRLNKINISIQMSSELLNSINKF-YKLKNEIKNF-SIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFT-------DINETISGHEQL--GISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLK 6223
+C SG C+ CI G+Y G +C +C+CN++ CD + G C CS+NT G C C G+YGD + C++C C +L S S C L G CD C + +C+ C +G Y + +KC C C S C+ +G+C C+ + +G CE+C +GYF +C+ C C + ++S+ C++ G+C C +NV+ CD+C+ H N+ C C C + GS++ C+ E G C+C V+ C +C G++ GC C + CN E G C+CPP+T G C C ED W + GCK C C ++GS S+ C+ ++G CAC+ F KCDRC GY FP C C+C NGT+ +EL C + GQC CK NV G C C+ GTF G GC CFCFGVS C + V S H+ D K +LE Y T + ++ P Y + + F L + + +V+ LD Q + L + S V EH KW +Y N + +K + D I SNL +I ++ + + + LY N I + T + VE C C G CQ GYY+++ + N M+ R ++ C CN +S CD TG C C N G C +C G+Y K + C C CP +F+ C +E + +C C+ GY G+ CE C G+YGNP + G C+ C C + GS C+ + G C+C GV G CDQC +V+ C SC GCT LL +D + ++ +N L P +L + L +Q+ + L ++ L E++ S + EK I+ L +R ++LR+ D+ E + + L IS L ++ +E + + +LK++ ++E +++ S + + NL+ ++ ++ D + + TM ++++ EL R K + + ++TK RD L + + + + +++ E + + + + N++S V+ + I D + + Q L+ + L+ + +H + ++ +++ V+ +N + +D N + + DSL L N + +VE + ++K + L K
Sbjct: 648 ECPWGYSGTSCEYCISGFYRVGGILFGGNCLQCECNDHATECD-INGVCLGCSHNTTGPHCDQCLPGYYGDPTEGTAEDCQRCACPLTLASNNFSPTC-SLQGPGEIMCDQCEQGYTGTKCERCANGYYGNPTVAGQKCSVCECNGNVDPSEPGHCDAVTGECLKCVGHTSGRHCERCEDGYFGDAVTAKDCQACGCHANGSVSSVCNIITGQCDCRANVVGDKCDRCQVGFHGINSGDGCRQCECSQSGSVSEACE-EDGQCQCIVGVSGAKCDRCQQGFYNFTDGGCTPCDCAHTHGNCNAETGECICPPHTRGEKCEVCEEDHWGHDAVTGCKPCECSVDGSNSSQCDLLNGQCACRPHFASQKCDRCALGYRKFPECTACNCNTNGTREDFCDDELEVCGCEDHGQCVCKDNVGGGGCDECKTGTFGLWGQNPSGCSPCFCFGVSTVCEELGGLVRVPVSAERLHVVSQSDLKGTLEG--VYHTEGEMLLDVTQLQSASSLTGPYYWRLPQRFQGSKLLSYGGDLSYSVAFAALDGSGLSNHEPQVLIRGGHLKKLVIYMDMPAPENSIRTTQRVPLTEH----KW---KYFNAVSEKAVSHADFMSILSNLHINILVL----MANYLLFLYMLISN-----ISMDTAVEADEAPEGTPEARLVEACECPPGYAGLSCQECAPGYYRQHVSELN------MKGMKRPMMQPCVPCQCNNHSLACDLDTGKCLGCQHNTTGENCNVCAPGYYGQVKGSISDCSLCACPLHSNSFSPTCV-LEGVGDFRCDACKPGYEGRYCERCSLGYYGNPSEPGGKCEVCQCTESGSLHPVCDSQTGKCECKAGVRGHLCDQCEDRHVLSNDQCLSCDDGCTGLLLDDVDAVEASIQSLNLSGVILAPYSLLISLENQTQELQVGAFLEENVGNLNMTLTEEVEEVNSTTLMEQMMALIEKMKTID-LSSRNTTADDELRAAVAVLESIQKDVVEPSNRTQTLKEKISTALARHDQQLMEAQDLLNAAQSHNNHTEHLLKNIHTNIEEFTS-LRHNVSSLNQTTVTNLEDAQD--LLTDAFSIAGDLHNLTMKAEEEKDELVEWSPTLR-KHVDTLVMELTK-RDAL-QLVYRAEDHAQALSKEAQAVYNTLTGVREASTNITSASQVNSN--ISDNIHQAENQALSANQTASSALSLVSHTVH-----IQTLDDLAQNVSDVNGRLNLVRARVD-NFTQLLPDSLTALRNLSNGGRELVVEVKQQVEKANASLQK 1875 HSP 3 Score: 211.846 bits (538), Expect = 7.268e-54 Identity = 168/564 (29.79%), Postives = 246/564 (43.62%), Query Frame = 2
Query: 1208 GVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVH---VNSDDL----CKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSL--------SAKNLKI---SDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDS--PDVIIKCGDFR---LFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCL--VKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLAL--DEMPH-------IEKCNCPPGFNGLSCESCESGYRR--VNNVLYQGQ-------CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS-CKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLCK 2758
G CI C +T G CE C + + V C PC+C G+ S D G C C+ + CD C++GY ++P EC C C+ +G+ C C+ H V D++ C CK G F L NP GC PC+CFG++ C L SA+ L + SDLKG + E L T Q +S P YYW P + G+ + SYG Y V + L GS + + P V+I+ G + ++ + + ++ + + +WK +KA + +F+S L + ++ L++ A Y + L + +S + A+ DE P +E C CPPG+ GLSC+ C GY R V+ + +G C C C NHS CD G+C C HNT G +C CA GYYG K +++ C C CP + C ++ C C G +C+RC GYYGNP + C+ C+C + CD TG+C C G C C+
Sbjct: 950 GECI-CPPHTRGEKCEVCEEDHWGHDAVTG-----CKPCECSVDGSNSSQC-------DLLNGQCACRPHFASQKCDRCALGYRKFP--ECTACNCNTNGTREDFCDDELEVCGCEDHGQCVCKDNVGGGGCDECKTGTFGLWGQNPSGCSPCFCFGVSTVCEELGGLVRVPVSAERLHVVSQSDLKGTLEGVYHTEGEMLLDVTQLQSASS--------LTGP--YYWRLPQRFQGSKLLSYGGDLSYSVAF---AALDGSGLSNHEPQVLIRGGHLKKLVIYMDMPAPENSIRTTQRVPLTEHKWKYFNAVSEKAVSHADFMSILSNLHINILVLMANY------LLFLYMLISNISMDTAVEADEAPEGTPEARLVEACECPPGYAGLSCQECAPGYYRQHVSELNMKGMKRPMMQPCVPCQCNNHSLACDLDTGKCLGCQHNTTGENCNVCAPGYYGQVKG-SISDCSLCACPLHSNSFSPTCVLEGVGDFRCDACKPGYEGRYCERCSLGYYGNPSEPGGKCEVCQCTESGSLHPVCDSQTGKCECKAGVRGHLCDQCE 1478 HSP 4 Score: 100.138 bits (248), Expect = 4.847e-20 Identity = 77/265 (29.06%), Postives = 121/265 (45.66%), Query Frame = 2
Query: 4313 LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPS--AHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQ--QGSQSLNCNEF-GIC-QCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
+ RD M L +V SL+++ + + ++++ ++ V + VE+C C G C+ ISG+Y+ G + G L +C CN ++ CD + G C C N G C C G+Y + + + C+ C CP A NF+ C+ ++ E+ C QC GY+G CE C G+YGNP + G C C+CN S+ +C+ G C +C G C++C Y V K CQ+C
Sbjct: 592 VQRDELMSVLADVASLRIRAHLNTSVDGVLRLSSVSLAVADPISRSAAPAPDVEQCECPWGYSGTSCEYCISGFYR----------VGGILFGGNCL----QCECNDHATECD-INGVCLGCSHNTTGPHCDQCLPGYYGDPTEGTAEDCQRCACPLTLASNNFSPTCS-LQGPGEIMCDQCEQGYTGTKCERCANGYYGNPTVAGQKCSVCECNGNVDPSEPGHCDAVTGECLKCVGHTSGRHCERCEDGYFGDAVTAKDCQAC 840 HSP 5 Score: 93.9745 bits (232), Expect = 3.379e-18 Identity = 58/161 (36.02%), Postives = 81/161 (50.31%), Query Frame = 2
Query: 3407 GICV-CPPNTIGNLCNKCSEDSWDFNE-----TIGCKKCNCYINGSYSNICNK-----------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHA 3838
G+C+ C NT G C C++ + ++ T C +CNC + GS S++C K + G C CK F G KCDRC GY FP C C C+ G S+ + C +C CK NV+G C +C++G + + GC CFCFGVS+ C
Sbjct: 354 GVCINCTQNTAGVNCETCADGYYRPHKMSPYATEPCLECNCDMRGSVSHVCLKDDKHANPGKGLLPGQCVCKEGFAGEKCDRCAFGYRDFPLCTRCECSLKG--SHNIDPCR----ECICKVNVMGANCDLCKQGFYNLQASNPEGCTECFCFGVSDVCEG 508 HSP 6 Score: 61.2326 bits (147), Expect = 2.614e-8 Identity = 57/185 (30.81%), Postives = 79/185 (42.70%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKC-NCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGG--VCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERY--PNGECLPC 1489
C CNNH+ CD + KC C+HNT G NC C P + + ++ C C C SN S++ T +EG G C C+ EG CE+C G+Y + + C CQC G++ + D + G C CK G G CD C +R+ N +CL C
Sbjct: 1339 CQCNNHSLACD------LDTGKCLGCQHNTTGENCNVCAPGYYGQVKGSISDCSLCACPLHSN--SFSPTCV-----------LEGVGDFRCDACKPGYEGRYCERCSLGYYGNPSEPGGK---CEVCQCTESGSL-------HPVCDSQTGKCECKAGVRGHLCDQCE---DRHVLSNDQCLSC 1491 HSP 7 Score: 59.3066 bits (142), Expect = 9.999e-8 Identity = 57/223 (25.56%), Postives = 95/223 (42.60%), Query Frame = 2
Query: 4460 VTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYKNDK----NYDQCEPCQCPSAHYNFAQNCTRIESS-------LELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLN-CNEFGICQCSPGVLGIKCDQCAPNY 5038
VT +C+C T G C GY++E + + G++ GN +KCNC+ ++ C +V G C NC N G C+ C +G+Y+ K + C C C + + C + + L +C C+ G++G+ C+ C G+ P+ C C+C+ +GS +++ C E C C V+G CD C +
Sbjct: 279 VTKKLQCVCEHNTCGESCNECCPGYHQELW------QPGTISDGN----TCEKCNCHNKAEDCYYNQTVADLKMSLNTHGQFVGGGVCINCTQNTAGVNCETCADGYYRPHKMSPYATEPCLECNC-DMRGSVSHVCLKDDKHANPGKGLLPGQCVCKEGFAGEKCDRCAFGYRDFPL----CTRCECSLKGSHNIDPCRE---CICKVNVMGANCDLCKQGF 483
BLAST of Laminin subunit alpha-2 vs. Ensembl Cavefish
Match: ENSAMXT00000010400.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001664.1:1364:148130:-1 gene:ENSAMXG00000009442.2 transcript:ENSAMXT00000010400.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 554.673 bits (1428), Expect = 6.960e-169 Identity = 389/1117 (34.83%), Postives = 541/1117 (48.43%), Query Frame = 2
Query: 200 AKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNET--KRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSC---YNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT----HKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCD-KVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVA-----VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSN-GEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTV---YYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGF--------NGLSCESCESGYRRVNNVLYQGQCEACACY------NHSNTCDSLNGE---CQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPC-PRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY-LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY---PNCKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDME---------TGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY 3382
AKIT++ATCG PE+FC+LV+ + Q + NP C +C+ ++E +RHPIE+A D N WWQSPS+ G++Y V +T+DL+QV+QI Y +LK +SPRPGNWILERSLDGE + PW ++A T C +N+ R P P DDE+ CT++YS+ L +GEI SLINGR S + TL+NF +ARYIR +FL+ TL A+LMT ++ + RYYYS+++I +GG CIC HA C +I +S C C+HNTCG +C+ CCP +NQ+PW VCE+CNC+ KS EC YNQTVA +LS+N G EGGGVC C ENT G+NC+ C G+YRP V + YPC PC C G+I S C+ + + G G C CK+GY G CD C+ GY +P EC C CS+ GS+ C+ PC CK +V + C CK G++NL GCE CYC G+ + C L K ++D+ GW LT GE + T Y+ R P + ++W+APP YLGN +++ ++ + + L S F F V + + + D A+ E +C + S + +N T +G L H C+ G+ G C+ C GY C+ CAC N S TC C C G C+ C +GY+G S SC+ C C LD C + E C +C G CD C +GYYG+ C+ C+CN N E C+ G+C C N +G +C C L C C+C+S GS S +C + G+C C+ ++CD C G + ++ C PC C G C++T+GQC C N G+RC++C ++ Y CK C C+ ++S C++N G C C + C +C+ + + C+ C+C GS CD + TG C C NV CS C G FGL V+ C CY
Sbjct: 2 AKITANATCGERKPEMFCKLVEHVPGQPVRNPQ----CRMCNLKSERPYERHPIEYAI-DGTNKWWQSPSIKNGMEYHYVTITLDLQQVFQIAYFILKAANSPRPGNWILERSLDGENFTPWQYYAITDTECITRFNISPRTGP---------PGYRRDDEVICTSFYSKIHPLENGEIHTSLINGRPSADDPS--------PTLLNFTTARYIRLKFLRIRTLNAELMTLALNDPLDIDPIVTRRYYYSIKDISVGGMCICYGHAKACPLNLITKKLS---CECEHNTCGESCDRCCPGYNQKPWMAGTFLTRHVCEKCNCHGKSEECYYNQTVADGKLSLNVNGEYEGGGVCRGCSENTAGVNCQSCTDGYYRPRGVNPEDPYPCRPCSCDPEGSISSACIPDETQASGGLSEGWCRCKQGYTGEKCDRCAFGYTGFP--ECQRCNCSVFGSINQD--PCQLPCVCKDNVEGVN-CDRCKMGFYNLHGDGFHGCEKCYCSGVASNCEDLLWKRSNLTDMSGWFLTGAEGEGLVWANRN-----TPLQVYVSHRDAQPHLEAPFFWSAPPSYLGNKVST-----NHLDHLSINIYLNVSPF-----------FLYFCLCVCVCVCRLGSVSVEVASE-----GSSSDVQASAVEVCTCPFGYSGTSCE----SCAEGYRRINGTTYQGVCELCQCHG-HAAHCDEVTGYCLGCEDHTTGPHCDKCAPGYYGDATKGTASDCQPCACPLNAPSNNFSPTCHQDARSQMICDRCQPGYTGPRCDRCDNGYFG-RPSVLGGSCRPCECNDNLDLSTDRSCDPVTGE---CLRCREGYGGPVCDTCADGYYGDAIFAKDCQPCRCNINGSVSEVCNKQNGQCECRQNAIGRQCDEC---MVTHMLPGGCVPCNCNSFGSKSFDCGE-NGQCRCQPGVTGQKCDRCAPGHFNFQEGGCTPCQCAHVG-----NNCDSTTGQCICPPNTIGERCDRCAPNHWGYDITTGCKPCGCNVLGSVSQQCNVNTGCCTCQERFVGEKCTECK--LGFRDFPQCMSCDCTLAGSTADTCDSDSGLCACADRTGQCSCKANVEGVRCSSCKAGTFGLSVSNPLGCSHCY 1042 HSP 2 Score: 279.256 bits (713), Expect = 3.516e-76 Identity = 187/564 (33.16%), Postives = 262/564 (46.45%), Query Frame = 2
Query: 2240 IEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCP--RLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKK-KISCKRCKCNNN-----QESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC---HAILNHRIVENSIYEY 3880
+E C CP G++G SCESC GYRR+N YQG CE C C+ H+ CD + G C C +T G HC+ CA GYYGDA + C+ C CP + C + +C +C +G CDRC GY+G P SC+ C+CN+N SCDPVTGEC RC G C C G+YGDA+ K C+ C C+ GS SE C+K G+C+CR N + RQCD C ++ C+PCNC G S+ F +GQC C V G +C++C G+FN+ C C C+ + +CD G+C C N I CD+C H+ ++ T C PC C GS++ +C++ TG C C + C++C +G+ DF + C C+C + GS ++ C+ SG+CAC DR + CSC K NV G +C C+ GTF + GC C+CFG+S+ C ++ R+ + + Y
Sbjct: 599 VEVCTCPFGYSGTSCESCAEGYRRINGTTYQGVCELCQCHGHAAHCDEVTGYCLGCEDHTTGPHCDKCAPGYYGDATKGTASDCQPCACPLNAPSNNFSPTCHQDARSQMICDRCQPGYTGPRCDRCDNGYFGRPSVLGGSCRPCECNDNLDLSTDRSCDPVTGECLRCREGYGGPVCDTCADGYYGDAIFAKDCQPCRCNINGSVSEVCNKQNGQCECRQNAIGRQCDECMV-THMLPGGCVPCNCNSFG---SKSFDCGENGQCRCQPGVTGQKCDRCAPGHFNFQEGGCTPCQCA--HVGNNCDSTTGQCICPPNTIGERCDRCAPNHWGYDITTGCKPCGCNVLGSVSQQCNVNTGCCTCQERFVGEKCTECKLGFR--------------------------------------------DFPQ---CMSCDCTLAGSTADTCDSDSGLCACA--------DRTGQ----------CSC----------------------KANVEGVRCSSCKAGTFGLSVSNPLGCSHCYCFGMSQSCTEAQGLIRMRVSKTHTHTY 1069 HSP 3 Score: 82.8037 bits (203), Expect = 5.908e-15 Identity = 72/271 (26.57%), Postives = 119/271 (43.91%), Query Frame = 2
Query: 4292 SNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCP--SAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQ--QGSQSLNCNEF-GIC-QCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
N +++N + + LNV+ + + V + ++ ++ +V + + +AVE C C G C++ GY + + +T G + C C+G++ CD VTG C C + G C C G+Y + C+PC CP + NF+ C + S + +C+ GY+G C+ C G++G P + G C+ C+CN S +C+ G C +C G G CD CA Y + K CQ C
Sbjct: 541 GNKVSTNHLDHLSINIYLNVSPFFL-YFCLCVCVCVCRLGSVSVEVASEGSSSDVQASAVEVCTCPFGYSGTSCESCAEGYRR----INGTTYQGV----------CELCQCHGHAAHCDEVTGYCLGCEDHTTGPHCDKCAPGYYGDATKGTASDCQPCACPLNAPSNNFSPTCHQDARSQMICDRCQPGYTGPRCDRCDNGYFGRPSVLGGSCRPCECNDNLDLSTDRSCDPVTGECLRCREGYGGPVCDTCADGYYGDAIFAKDCQPC 796 HSP 4 Score: 77.411 bits (189), Expect = 2.800e-13 Identity = 49/147 (33.33%), Postives = 73/147 (49.66%), Query Frame = 2
Query: 4613 KKCNCN-----GYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+ C CN + CD VTG+C C G C C +G+Y + C+PC+C N + + + + +C+CR G+ C+ C + + G C C+CN GS+S +C E G C+C PGV G KCD+CAP +
Sbjct: 741 RPCECNDNLDLSTDRSCDPVTGECLRCREGYGGPVCDTCADGYYGDAIFAKDCQPCRC-----NINGSVSEVCNKQNGQCECRQNAIGRQCDEC---MVTHMLPG-GCVPCNCNSFGSKSFDCGENGQCRCQPGVTGQKCDRCAPGH 878 HSP 5 Score: 75.485 bits (184), Expect = 1.071e-12 Identity = 83/280 (29.64%), Postives = 110/280 (39.29%), Query Frame = 2
Query: 3086 CLCSPHALSADCDLNNGK--CKCGSNVINRICDKC---ENLHYWN-----NRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGSYSNIC---------NKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHR 3853
C+C HA + +L K C+C N CD+C N W R C CNC ++ C Q VA G L+V G + G VC C NT G C C++ + + + C+ C+C GS S+ C G C CK + G KCDRC GY GFP CQ C+C+ G+ + D C CK NV G C C+ G + G GC C+C GV+ C +L R
Sbjct: 239 CICYGHAKACPLNLITKKLSCECEHNTCGESCDRCCPGYNQKPWMAGTFLTRHVCEKCNC---------HGKSEECYYNQTVAD--------GKLSLNVNGEYE-----GGGVCRG-----CSENTAGVNCQSCTDGYYRPRGVNPEDPYPCRPCSCDPEGSISSACIPDETQASGGLSEGWCRCKQGYTGEKCDRCAFGYTGFPECQRCNCSVFGSINQ-----DPCQLPCVCKDNVEGVNCDRCKMGFYNLHGDGFHGCEKCYCSGVASNCEDLLWKR 486 HSP 6 Score: 67.3958 bits (163), Expect = 2.857e-10 Identity = 73/253 (28.85%), Postives = 101/253 (39.92%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVR------TSFYRCKP---PCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCS 1675
C CN+ K ENG +C C+ G C+ C P FN + C C C N C G CI C NT G C++C W T C PC C+ LG++ C N G C C++ + G C C +G+ +P +C+ C C++ GS + C C+CK +V C +CK G F L +NP GC CYCFG++ C+
Sbjct: 842 CNCNSFGSKSFDCGENG----QCRCQPGVTGQKCDRCAPGHFNFQE----GGCTPCQCAHVGNNCDSTT------------------GQCI-CPPNTIGERCDRCAPNH---WGYDITT--GCKPCGCNVLGSVSQQCNVN--------TGCCTCQERFVGEKCTECKLGFRDFP--QCMSCDCTLAGSTADTCDSDSGLCACADRTGQCSCKANVEGV-RCSSCKAGTFGLSVSNPLGCSHCYCFGMSQSCT 1051 HSP 7 Score: 59.3066 bits (142), Expect = 8.064e-8 Identity = 86/374 (22.99%), Postives = 142/374 (37.97%), Query Frame = 2
Query: 4316 TRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIG----------------KRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---DSVT---------------GDCQNCMGNREGNKCQLCREGFYK----NDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELK---CQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYV---------VEKKTCQSCYSGCTKDLLVRIDTLNETVINF---------KWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIK 5257
T + L + +L +L T +N PL I+T + + + I +T C C T G C GY ++ + +G+ T + +KCNC+G S+ C +V G C+ C N G CQ C +G+Y+ N ++ C PC C P + A ++S L C+C+ GY+G+ C+ C G+ G P C+ C+C+ GS + + + C C V G+ CD+C + EK C S C +DLL + L + F W + L + ++ + + + S Y L N++
Sbjct: 189 TARYIRLKFLRIRTLNAELMTLALNDPLDIDPIVTRRYYYSIKDISVGGMCICYGHAKACPLNLITKKLSCECEHNTCGESCDRCCPGYNQKPW------MAGTFLTRHVC----EKCNCHGKSEECYYNQTVADGKLSLNVNGEYEGGGVCRGCSENTAGVNCQSCTDGYYRPRGVNPEDPYPCRPCSCDPEGSISSACIPDETQASGGLSEGWCRCKQGYTGEKCDRCAFGYTGFP----ECQRCNCSVFGSINQDPCQL-PCVCKDNVEGVNCDRCKMGFYNLHGDGFHGCEKCYCSGVASNC-EDLLWKRSNLTDMSGWFLTGAEGEGLVWANRNTPLQVYVSHRDAQPHLEAPFFWSAPPSY-LGNKVS 545
BLAST of Laminin subunit alpha-2 vs. Ensembl Cavefish
Match: lamc1 (laminin subunit gamma 1 [Source:NCBI gene;Acc:103023755]) HSP 1 Score: 442.965 bits (1138), Expect = 6.685e-127 Identity = 317/1049 (30.22%), Postives = 483/1049 (46.04%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAK---SDDDNLWWQSPSMAQGLKYQN-VNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKI------SDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR-----LFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVL-YQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSY-SSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKI----SCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS----CKKCDCDSLGSTSEN--CDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--PNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRT 3214
N + ++ TCG+ PE +C + + C +CD R+ + H + + D WWQS +M G++Y N +N+T+ L + + I Y+ LK S I +R+ +G + P+ +++ + Y+ R + + T + + CT +S L G + S + GR S N + + L ++++A IR + T ++ + + + YYY++ + +GG+C CN HA +C +++ S CNCKHNT G +C C P +N RPW+ C CNCN KS EC ++ +++ R G GG C NC +NT+G NCE+C+ +YR + C PC C+ +G++ S DI G C CK G G CD C GY C PC C+ GS + C CK +V+ + C CK GYFNLD NP+GC PC+CF + C S ++ D +GWK G + + + + E S I + + Y+ AP +LGN + SYG ++E + + + + DV+++ + + S ++Q VF + T++ + T +F L + + I+ YS + + L N++L + +EKC CPPG+ G C C GYRR L CE C C HS TCD G C NC NTAG CE C G+YGDA + + CK CPCP C+ ++ VC C T+G C+ C +G++G+P K +C+ CKC++N + +CD TGEC +C YNT G C CK GFYG+AL + CK C C G+ + C ++TG+C C + NR C +C G Y K C CNC G S+ C+ SGQC C V G +CE+C +F + CK C C P +G+C+C + CD CE +++N T
Sbjct: 47 NVTVVATNTCGSP-PEEYCVQTGVTGVTKS--------CHICDARDPRQHHSAVYLTDYNNQADTTWWQSQTMLAGIQYPNSINLTLHLGKAFDITYVRLKFHTSRPESFAIYKRTREGGPWTPYQYYSGSCEKTYSKLNRGF----IRTGED-----EQQALCTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPV------LQDWVTATDIRVTLNRLNTFGDEV-----FNDPKVLKSYYYAISDFAVGGRCKCNGHASEC---VKDEYSKLVCNCKHNTEGDDCGVCKPFYNDRPWRRATADNPNECLPCNCNGKSGECYFD----------SELYRATGHGGHCRNCADNTDGPNCERCLDNYYRDGSGQR-----CLPCSCNPVGSL----STQCDI----TGRCSCKSGVMGDKCDRCQPGYHTLTEAGCSPCSCNPAGSTQECDVNTGR-CQCKDNVDGFN-CDRCKLGYFNLDPQNPQGCTPCFCFHHSTVCESADGYSIHTITSNFDRDDEGWKGKQRDGSSVPV----QWSLASQEISLISDDY---FPMYFVAPAKFLGNHLLSYGQNLTLNFRVE----RRDARLSAEDVMLEGAGLQVAVPLIAQGNSYPGEQMQTFVFRLHQTTDYPWRPVL-----THGDFQKLLHNLTSIKIRGT-YSERSAGYLDNVSLVSAQRGPGTPAR-WVEKCTCPPGYVGQHCGQCALGYRRSRPELGLYSPCEPCNCNGHSETCDPATGMC-NCQDNTAGMSCERCKDGFYGDATVGSSSDCKPCPCPS-----GATCALVPKTKEAVCTNCPAGTTGKRCELCDDGFFGDPLGKSGPVRACRECKCSSNIDPNAVGNCDRETGECLKCIYNTDGFFCDRCKEGFYGNALATNVADKCKPCSCSPYGTVDQQTTCMQVTGECTCLPHVTNRDCSACQPGFYNLQSGKGCERCNCNQIG--STNGHCDIVSGQCECQPGVTGQQCERCEVNFFGFSTSGCKPCDCDPEGSRLAQCREDGRCECRPGFVGARCDMCEENYFYNRST 1012 HSP 2 Score: 165.236 bits (417), Expect = 6.498e-40 Identity = 158/562 (28.11%), Postives = 239/562 (42.53%), Query Frame = 2
Query: 3419 CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYP-RRFGWPLTMFRPIY-VNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQ---YQNDILDKFIILEDSNV---INSNLTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI-KGIP---CKSCDC--NQQGSQSLNCN-EFGIC-QCSPGVLGIKCDQCAPNY 5038
C NT G C +C ++ + C C+C GS S C+ I+G C+CK+ G KCDRC GY+ C PCSC G+ +CD TG+CQCK NV G C C+ G F D GC CFCF S C + + I ++I D++ +D S++ + + + + P+Y V K +H LS + +++ +N V+ D + E +V +E G++ V +I+Q Y + + F+ LT F L N+TS+K++ Y+++ L + +++ + + G VEKC C G HC GY + L + + CNCNG+S+ CD TG C NC N G C+ C++GFY + + C+PC CPS C + + E C C G +GK CE C GF+G+P+ K P C+ C C N + NC+ E G C +C G CD+C +
Sbjct: 357 CADNTDGPNCERCLDNYYRDGSGQRCLPCSCNPVGSLSTQCD-ITGRCSCKSGVMGDKCDRCQPGYHTLTEAGCSPCSCNPAGSTQ----ECDVNTGRCQCKDNVDGFNCDRCKLGYFNLDPQNPQGCTPCFCFHHSTVCESADGYSI--HTITSNFDRDDEGWKGKQRDGSSVPVQWSLASQEISLISDDYFPMYFVAPAKFLGNHLLS-YGQNLTLNFRVERRDARLSAE-----DVMLEGA----GLQVAVP----------------------LIAQGNSYPGEQMQTFVFRLHQTTDYPWRPVLTHGDFQKLLHNLTSIKIRGTYSERSAGYLDNVSLVSAQ-----RGPGTPARWVEKCTCPPGYVGQHCGQCALGYRRSRPELG-------------LYSPCEPCNCNGHSETCDPATGMC-NCQDNTAGMSCERCKDGFYGDATVGSSSDCKPCPCPS-----GATCALVPKTKEAVCTNCPAGTTGKRCELCDDGFFGDPLGKSGPVRACRECKCSSNIDPNAVGNCDRETGECLKCIYNTDGFFCDRCKEGF 855 HSP 3 Score: 158.688 bits (400), Expect = 5.541e-38 Identity = 110/338 (32.54%), Postives = 157/338 (46.45%), Query Frame = 2
Query: 2594 GVHCDRCIEGYY-GNPKKKI--SCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN-------YPNCKKCLCSPHAL---SADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC-NCY 3514
G HC +C GY P+ + C+ C CN + E+CDP TG C C NT G C+ CK GFYGDA S CK C C S + + C +C ++C+ C G + + C C C ++ C+ +G+C C+ N +G C++C EG++ CK C CSP+ C G+C C +V NR C C+ Y + C CNC + GS N CD+ +G C+C V + C +C+V +FG +GCK C P C +G C C P +G C+ C E+ + T GC++C NCY
Sbjct: 685 GQHCGQCALGYRRSRPELGLYSPCEPCNCNGHSETCDPATGMCNCQDNTAGMSCERCKDGFYGDATVGSSSDCKPCPCPSGATCALVPKTKEAVCTNCPAGTTGKRCELCDDGFFGDPLGKSGPVRACRECKCSSNIDPNAVGNCDRETGECLKCIYNTDGFFCDRCKEGFYGNALATNVADKCKPCSCSPYGTVDQQTTCMQVTGECTCLPHVTNRDCSACQPGFYNLQSGKGCERCNCNQIGSTNGHCDIVSGQCECQPGVTGQQCERCEVNFFGFSTSGCKPCDCDPEGSRLAQCREDGRCECRPGFVGARCDMCEENYFYNRSTPGCQQCPNCY 1022 HSP 4 Score: 117.472 bits (293), Expect = 2.065e-25 Identity = 98/356 (27.53%), Postives = 152/356 (42.70%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKDKK-------CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYF------NYPNCKKCLCSPHALSADCDLNNGKC--KCGSNVINRICDKCENLHYWNNRTDCVP------CNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFG--LDVTGCKKCPPCY---NGQVCNNE------GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC 3805
KC C +V + C C G Y + + C PCNC + C+ +G C C +N G CE+C +G++ + +CK C C A A C + + C+ C++ + + P C C +++P CD ETG C KC N C +C G++G L KC PC G V G C C P+ C+ C ++ GC++CNC GS + C+ +SG C C+ G +C+RC ++GF C+PC C G++ L +C + G+C+C+ +G +C +C + F + T GC C
Sbjct: 676 KCTCPPGYVGQHCGQCALG-YRRSRPELGLYSPCEPCNCN-----GHSETCDPATGMCNCQDNTAGMSCERCKDGFYGDATVGSSSDCKPCPCPSGATCALVPKTKEAVCTNCPAGTTGKRCELCDDGFFGDPLGKSGPVRACRECKCS-SNIDPNAVGNCDRETGECLKCIYNTDGFFCDRCKEGFYGNALATNVADKCKPCSCSPYGTVDQQTTCMQVTGECTCLPHVTNRDCSACQPGFYNLQSGKGCERCNCNQIGSTNGHCDIVSGQCECQPGVTGQQCERCEVNFFGFSTSGCKPCDCDPEGSR---LAQC-REDGRCECRPGFVGARCDMCEENYFYNRST--PGCQQC 1018 HSP 5 Score: 102.834 bits (255), Expect = 5.571e-21 Identity = 131/544 (24.08%), Postives = 208/544 (38.24%), Query Frame = 2
Query: 4484 CNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNG-----YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN---DKNYDQCEPCQCPS---------------------------------AHYNFAQ-------NCTRIESS------LELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKT-----CQSCYSGCTK----------------DLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSI--------NKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDI------------NETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKY-------VDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKE---YLNTMSTKK---KEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQ 5791
C A T G C+ G++ + KSG +R ++C C+ CD TG+C C+ N +G C C+EGFY N D+C+PC C YN NC +I S+ + +C+C+ G +G+ CE C+ F+G G CK CDC+ +GS+ C E G C+C PG +G +CD C NY + T C +CYS D L DT+++ + K + L + S + LL+ + ++ +L+N I+N + D A+ + E EKL R L+ + + + N T+ E ++ + A +T K+ T A ++LK L +DELN+ KY+++ D NL+ +K + A+ NK Y N S K E I + DL K+ K K+ ++LR ++
Sbjct: 782 CPAGTTGKRCELCDDGFFGDPLG-----KSGPVRA-------CRECKCSSNIDPNAVGNCDRETGECLKCIYNTDGFFCDRCKEGFYGNALATNVADKCKPCSCSPYGTVDQQTTCMQVTGECTCLPHVTNRDCSACQPGFYNLQSGKGCERCNCNQIGSTNGHCDIVSGQCECQPGVTGQQCERCEVNFFGFSTSG--CKPCDCDPEGSRLAQCREDGRCECRPGFVGARCDMCEENYFYNRSTPGCQQCPNCYSLVRDKVNQHRQKLHDLQTLIDNLGTQDTVSDKAFEDRLKEAEKAIMDLLKEAQASKDVDKGLLDRLKGINNTLTTQWNRLQN-IRNTVDNTGTLADKAQNRVREAEKLIDRARDELDKAKDAISKVDIKTPTTTGDPNNMTLLAEEARNLAEKHKLEADQIEKTAKDAND---TSTKAYNLLKKTLDGENKINSDIDELNK--KYSEAK---DLAKNLEKQASK-VQAEAEEAGNKALKIYANLTSLPPFDVKSLEDEASKIKKEASDLDKLIDKTEKEYNDLRDDLK 1301 HSP 6 Score: 98.9821 bits (245), Expect = 8.954e-20 Identity = 76/255 (29.80%), Postives = 115/255 (45.10%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-----CRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGR---ISSEKFCNTT-SGQCF-CLENVNGDRCEKCVEGYFNYPNCKKCL---CSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY 3382
RCKCN + C V E C C +NT G +C +CK FY D + +T++N +C+PCNC + SE + T G C C +N +G CE+C++ Y+ + ++CL C+P +LS CD+ G+C C S V+ CD+C+ ++ C PC+C +CD+ TG C+C NV +C +C +GYF LD + C PC+
Sbjct: 272 RCKCNGHASEC--VKDEYSKLVCNCKHNTEGDDCGVCKP-FYNDRPWRR----------ATADN-----------------------------PNECLPCNCNGKSGECYFDSELYRATGHGGHCRNCADNTDGPNCERCLDNYYRDGSGQRCLPCSCNPVGSLSTQCDIT-GRCSCKSGVMGDKCDRCQPGYHTLTEAGCSPCSCNPAGSTQECDVNTGRCQCKDNVDGFNCDRCKLGYFNLDPQNPQGCTPCF 483 HSP 7 Score: 68.1662 bits (165), Expect = 1.964e-10 Identity = 84/312 (26.92%), Postives = 127/312 (40.71%), Query Frame = 2
Query: 4295 NVINSNLTRDIFMMALLNVTSLKVKLYTDKV--NPPLIK---IKIITYKVMETTQAIGKRVTAVEK------CICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC--DSV-------TGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGS-QSLNCNEFGICQCSPGVLGIKCDQCAPNYV-VEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLD 5164
N NS + +D + VT ++ + D+V +P ++K I + V + G V+ C C T G+ C G K ++N + + N L CNCNG S C DS G C+NC N +G C+ C + +Y+ D + +C PC C + + C + +C C+ G G C+ CQ G++ G C C CN GS Q + N G CQC V G CD+C Y ++ + Q GCT T+ E+ + T+ S D
Sbjct: 220 NFDNSPVLQDWVTATDIRVTLNRLNTFGDEVFNDPKVLKSYYYAISDFAVGGRCKCNGHASECVKDEYSKLVCNCKHNTEGDDC-----GVCKPFYN-DRPWRRATADNPNECL----PCNCNGKSGECYFDSELYRATGHGGHCRNCADNTDGPNCERCLDNYYR-DGSGQRCLPCSCNPVG-SLSTQC-----DITGRCSCKSGVMGDKCDRCQPGYHTLTEAG--CSPCSCNPAGSTQECDVNT-GRCQCKDNVDGFNCDRCKLGYFNLDPQNPQ----GCTPCFCFHHSTVCESADGYSIHTITSNFD 507
BLAST of Laminin subunit alpha-2 vs. Ensembl Cavefish
Match: lamc3 (laminin, gamma 3 [Source:ZFIN;Acc:ZDB-GENE-060531-121]) HSP 1 Score: 383.645 bits (984), Expect = 1.559e-107 Identity = 303/1020 (29.71%), Postives = 449/1020 (44.02%), Query Frame = 2
Query: 311 CDVCD-NRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQN-VNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLS---AKNLK---ISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIF--------RYVVKIELQSTLKGSLIDSPDVIIKCGDFRL----FHSVSIEDRRLQRQVFIYIKPTEWK--IGKLTPDKA--ATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGY-RRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS----CKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDAL------TKSCKKCDCDSLGS--TSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRT 3214
CD D N + +F SD++ WWQS SM G++Y N VN+T+ L + ++I Y+ LK S RP ++ + + D PW PY Y+ R+ + +P + CT +S L G + S + G+ S N P L +++A + + T + + + + Y+Y++ + +GG+C CN HA +C + N C C+H+T G +C+ C P + RPW C CNC+ +++EC ++ A LS + GG CI C++NT G +CE C FYRP + L PC C C+ +G+ + + G C C+ G+ CD+C G+ G C C C GSV CNCK+ V + C C+ G FNL NP+GC+ C+C+G + CSS S A N+ + D GW + F+G++ + L E ++ Y+ AP ++GN + SYG R ++ ++ L+GS I + D S + R ++R V + P E++ + LT + A + + S L V Y + S RG + P +E C CPPGF G CE C G+ R N +C C C H TC G C +CT T G CE C GYYG+ + + N+C CPCP + ++ VC C N G C+ C +G+YGNP + C RC+CN N + CD VTG C +C +T G C+ C+ GF+G AL ++ CK CDC G+ S CD TG+C C + R C C +G + C CNC G SS C+ +GQC C V G +C+ C G+F + + C+ C C P S+ NG C C + CDKCE HY N+ T
Sbjct: 79 CDALDLETNHNATYLTDF-HSDEEPTWWQSQSMFYGVQYPNSVNLTLHLGKAFEITYVRLKFYTS-RPESFAIYKRTDET--APW-----QPYQYYSASCRKTYGRETRGFIRPGE-EERTAVCTDEFSDISPLTGGNVAFSTLEGKPSAYN-----FDQSP-ALQEWVTATDLLISLNRLNTFGDEF-----FKDAKVLRSYFYAISDFSVGGRCKCNGHASEC---MPNEDGHLVCVCEHHTAGKDCQQCAPFYLDRPWARATADSANQCVMCNCSGRADECVFD---AEQYLSTSS------GGRCIGCRDNTAGPHCEHCRDNFYRP-----SPLLPCYNCSCNTMGSA--------SLQCDEEGVCACRPSVMGLKCDVCKAGFHSLSPGGCRLCKCDERGSVGVCSTE-DGRCNCKLSVEGNS-CDRCRPGSFNLQQDNPDGCQTCFCYGHSLACSSSSRHVAFNITSDFLEDPDGW-VGEFSGQQQS-------PLLWKEGEVYLLPYSEKDAGYYKAPEKFIGNQLLSYGQHLSLSFTAEARELLPRKVTVVLEGSGISVSASLYSEQDLESGTGHIPSNTFSLRLIERDVHPALSPFEFRRMLSNLTALRISNAGGQNYTSQLSGVSLASAFPIAYPSDSS---------RGPL------APWVEMCTCPPGFMGQFCEQCAPGFTRETPNTGPFSRCVPCDCNQH-GTCHPETGVC-DCTDFTTGVSCERCLDGYYGNPLTGSPNACLPCPCP----DRSSCVQILETKAVVCTNCPANQRGTRCELCEDGFYGNPLGQDGVVRPCVRCECNGNIDPNSVGICDHVTGHCLKCLGHTAGDHCERCQKGFFGSALDSTLGPSQKCKPCDCYPAGTFGLSNECDPDTGRCMCLNHVTGRDCGQCQAGYFNLQPGVGCERCNCNPVG--SSSPACHPITGQCTCRTGVEGKQCDICRMGFFGFSSRGCRACNCDPMGSSSMQCHENGTCPCRQGFVGYKCDKCELNHYLNHVT 1019 HSP 2 Score: 135.576 bits (340), Expect = 5.750e-31 Identity = 107/339 (31.56%), Postives = 152/339 (44.84%), Query Frame = 2
Query: 2594 GVHCDRCIEGYYGNPKKKISCKRC-KCNNNQE-SCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLY----LKDKKCIPC-NCEIQGRI--SSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP---------NCKKCLCSP---HALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCN-CY 3514
G C++C G+ RC C+ NQ +C P TG C C+ T G C+ C G+YG+ LT S C C C S + + C +C N +C+ C G Y +D PC CE G I +S C+ +G C CL + GD CE+C +G+F CK C C P LS +CD + G+C C ++V R C +C+ ++ C CNC GS +P C TG C C V + C C +G+FG GC+ C P + C+ G C C +G C+KC + + + T C++C CY
Sbjct: 691 GQFCEQCAPGFTRETPNTGPFSRCVPCDCNQHGTCHPETGVCDCTDFTTGVSCERCLDGYYGNPLTGSPNACLPCPCPDRSSCVQILETKAVVCTNCPANQRGTRCELCEDGFYGNPLGQDGVVRPCVRCECNGNIDPNSVGICDHVTGHCLKCLGHTAGDHCERCQKGFFGSALDSTLGPSQKCKPCDCYPAGTFGLSNECDPDTGRCMCLNHVTGRDCGQCQAGYFNLQPGVGCERCNCNPVGSSSPACHPITGQCTCRTGVEGKQCDICRMGFFGFSSRGCRACNCDPMGSSSMQCHENGTCPCRQGFVGYKCDKCELNHYLNHVTHQCEECPVCY 1029 HSP 3 Score: 130.568 bits (327), Expect = 2.011e-29 Identity = 101/338 (29.88%), Postives = 154/338 (45.56%), Query Frame = 2
Query: 2858 CDCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP---NCKKCLCSPHALSADC----DLNNGKCK-CGSNVINRICDKCENLHYWNNRTD------CVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFG--LDVT--GCKKCPPC---------YNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
C C F+ + C+ C G + +C+PC+C G C+ +G C C + G CE+C++GY+ P + CL P + C + C C +N C+ CE+ Y N CV C C G+++P CD TG+C KC + A C +C G+FG LD T +KC PC + + + G C+C + G C +C ++ +GC++CNC GS S C+ I+G C C+T G +CD C G++GF + C+ C+C G+ S +C + G C C+Q +G KC C
Sbjct: 683 CTCPPGFMGQFCEQCAPGFTRETPNTGPFSRCVPCDCNQHGT------CHPETGVCDCTDFTTGVSCERCLDGYYGNPLTGSPNACLPCPCPDRSSCVQILETKAVVCTNCPANQRGTRCELCEDGFYGNPLGQDGVVRPCVRCEC-NGNIDPNSVGICDHVTGHCLKCLGHTAGDHCERCQKGFFGSALDSTLGPSQKCKPCDCYPAGTFGLSNECDPDTGRCMCLNHVTGRDCGQCQAGYFNLQPGVGCERCNCNPVGSSSPACHPITGQCTCRTGVEGKQCDICRMGFFGFSSRGCRACNCDPMGSSS---MQCHE-NGTCPCRQGFVGYKCDKC 1009 HSP 4 Score: 118.242 bits (295), Expect = 1.156e-25 Identity = 139/635 (21.89%), Postives = 268/635 (42.20%), Query Frame = 2
Query: 4616 KCNCNG-----YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY-----DQCEPC---------------------------------QCPSAHYNFAQ-------NCTRIESS------LELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKT-----CQSCYS---GCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYK---LKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETI-------SGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVL-KYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMS-TKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLS---SNHLVSKS--------LIIPDTLKKHVEQQLNKSSNLIK-KLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSL-----------KKLLAEFNSNKSEIVENIKNIDKEHEKLLK 6223
+C CNG +CD VTG C C+G+ G+ C+ C++GF+ + + +C+PC QC + ++N NC + SS + +C CR G GK C+ C+ GF+G +G C++C+C+ GS S+ C+E G C C G +G KCD+C N+ + T C CYS L V++ L + N+ ++ L +R N + Q + L N +N L N +++F + Q+ +D ++ ++E T + LR+ T +N ++ G E +G + T + T + + + L + V+ VL K + N ++ +++ ++S + ++I + + + +N +++ Y +S + EL+I + E++ ++Q++ +T Q + + + ++ + D K +++ + +L KN++ +N S S + DTL + EQ +NK+ +K +L K + Q + A+ I K E + + NI+ + K L + + +I+ +K + E+ LK
Sbjct: 819 RCECNGNIDPNSVGICDHVTGHCLKCLGHTAGDHCERCQKGFFGSALDSTLGPSQKCKPCDCYPAGTFGLSNECDPDTGRCMCLNHVTGRDCGQCQAGYFNLQPGVGCERCNCNPVGSSSPACHPITGQCTCRTGVEGKQCDICRMGFFGFSSRG--CRACNCDPMGSSSMQCHENGTCPCRQGFVGYKCDKCELNHYLNHVTHQCEECPVCYSLIRDQVSKLKVKLHDLERLLSNYDCRSR-HRLHVRPNHVRQHQQ--NVLDNQVNDHQGEDYLPNALEDF-LAIQEARDAFMSQFSQLE---TSAQTLELQLRNLATAVNCSLVVEGGSEQGEEAMGANECRAVVDTFVVVTDTQVRLQRMTLDLNSLVIPFVLPKGPTQWNALVNESEALVKSHKEMAIRI---EELAGEAMNVSSRTYSMLLSLLEDNSTELHIQDLTEQLSEMQELKENLTLQAN---ETLAAYLSVEEQHADAKAILNNITASLPELHKNVTTTEANATFSSSQQYNFWPLWFLLDTLIQVKEQLVNKTREEMKPELNKTLKTIQEFHQLTALTQGLKE--AALSSVIRGKKTESEAFSLHKNIEGIHQDWPVLQIQTKAALKRESIMEEKILTEVKKKVRHAERALK 1436 HSP 5 Score: 116.701 bits (291), Expect = 3.951e-25 Identity = 139/566 (24.56%), Postives = 228/566 (40.28%), Query Frame = 2
Query: 3407 GICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLE--SGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGRE-IPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNP-PLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI--KGI--PCKSCDCNQQGSQSLNCNEFGIC--------QCSPGVLGIKCDQCAPNY 5038
G C+ C NT G C C ++ + + + C C+C GS S C++ G+CAC+ + G KCD C G++ C+ C C E G+ + C G+C CK +V G C CR G+F GC +CFC+G S C + H V +I + D + SG+ S + + P + Y + F + L + + + ++ + + RE +P + V +E +G+ + S + H + + + + D+ E ++ SNLT L +++ + YT +++ L I Y + + G VE C C G C+ G+ +E PN+ C+CN + C TG C +C G C+ C +G+Y N + + C PC CP +C +I + + C C G CE C+ GFYGNP+ G+ PC C+CN +++ N GIC +C G C++C +
Sbjct: 352 GRCIGCRDNTAGPHCEHCRDNFYRPSPLLPCYNCSCNTMGSASLQCDE-EGVCACRPSVMGLKCDVCKAGFHSLSPGGCRLCKCDERGS----VGVCSTEDGRCNCKLSVEGNSCDRCRPGSFNLQQDNPDGCQTCFCYGHSLACSSSSRH--VAFNITSDFLEDPDGWVGEFSGQQQSPLLWKEG--EVYLLPYSEKDAGYYKAPEKFIGNQLLSYGQHLSLSFTAE------ARELLPRKVTVVLEG-SGI------SVSASLYSEQDLESGTGHIPSNTFSLRLIERDVHPALSPFEFRRML-SNLT-------ALRISNAGGQNYTSQLSGVSLASAFPIAYP---SDSSRGPLAPWVEMCTCPPGFMGQFCEQCAPGFTRE---TPNTGP----------FSRCVPCDCNQHG-TCHPETGVC-DCTDFTTGVSCERCLDGYYGNPLTGSPNACLPCPCPD-----RSSCVQILETKAVVCTNCPANQRGTRCELCEDGFYGNPLGQDGVVRPCVRCECN----GNIDPNSVGICDHVTGHCLKCLGHTAGDHCERCQKGF 860 HSP 6 Score: 67.3958 bits (163), Expect = 2.928e-10 Identity = 47/151 (31.13%), Postives = 69/151 (45.70%), Query Frame = 2
Query: 4616 KCNCNGYSKVCDSVTGDCQNCM--GNREGNKCQLC------REGFYKNDKNYDQCEPCQCPSAHYNFAQNCTR-IESSLELKC-QCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+C CNG++ C C+ + G CQ C R + +QC C C + + + +S +C CR +G CE+C+ FY P +PC +C CN GS SL C+E G+C C P V+G+KCD C +
Sbjct: 272 RCKCNGHASECMPNEDGHLVCVCEHHTAGKDCQQCAPFYLDRPWARATADSANQCVMCNCSGRADECVFDAEQYLSTSSGGRCIGCRDNTAGPHCEHCRDNFY-RPSPLLPCYNCSCNTMGSASLQCDEEGVCACRPSVMGLKCDVCKAGF 421 HSP 7 Score: 63.1586 bits (152), Expect = 5.937e-9 Identity = 62/205 (30.24%), Postives = 87/205 (42.44%), Query Frame = 2
Query: 4478 CICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTG--NRILINLKKCNCNGYS---KVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYD----QCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGY-SGKSCENCQQGFYGNPIKGI-----PCKSCDCNQQGSQSLN--CN-EFGICQCSPGVLGIKCDQCAPNY 5038
C C T G C+ + GYY G+ TG N L C C S ++ ++ C NC N+ G +C+LC +GFY N D C C+C + N N I + C +G+ +G CE CQ+GF+G+ + CK CDC G+ L+ C+ + G C C V G C QC Y
Sbjct: 732 CDCTDFTTGVSCERCLDGYY------------GNPLTGSPNACL----PCPCPDRSSCVQILETKAVVCTNCPANQRGTRCELCEDGFYGNPLGQDGVVRPCVRCEC---NGNIDPNSVGICDHVTGHCLKCLGHTAGDHCERCQKGFFGSALDSTLGPSQKCKPCDCYPAGTFGLSNECDPDTGRCMCLNHVTGRDCGQCQAGY 917 HSP 8 Score: 61.6178 bits (148), Expect = 1.878e-8 Identity = 56/211 (26.54%), Postives = 83/211 (39.34%), Query Frame = 2
Query: 4478 CICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKT----CQSCY 5071
C+C T G CQ F L + + N+ ++ CNC+G + C S G C C N G C+ CR+ FY+ C C C + ++ E C CR G C+ C+ GF+ G C+ C C+++GS + E G C C V G CD+C P ++ CQ+C+
Sbjct: 292 CVCEHHTAGKDCQQCAP------FYLDRPWARATADSANQCVM----CNCSGRADECVFDAEQYLSTSSGGRCIGCRDNTAGPHCEHCRDNFYRPSPLLP-CYNCSCNT------MGSASLQCDEEGVCACRPSVMGLKCDVCKAGFHSLSPGG--CRLCKCDERGSVGVCSTEDGRCNCKLSVEGNSCDRCRPGSFNLQQDNPDGCQTCF 483 HSP 9 Score: 58.151 bits (139), Expect = 1.960e-7 Identity = 63/226 (27.88%), Postives = 87/226 (38.50%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE---CRCSYNTMGTECQLCKHGFYGD-----ALTKSCKKC--------------DCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKDKKCIPC-NCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY---WNNRTDCVPCNCGRGSL 3250
RCKCN + C P C C ++T G +CQ C FY D A S +C D + STS G+C CR N C+ C Y + +PC NC S+ C+ G C C +V G +C+ C G+ + C+ C C C +G+C C +V CD+C + +N C C C SL
Sbjct: 272 RCKCNGHASECMPNEDGHLVCVCEHHTAGKDCQQCA-PFYLDRPWARATADSANQCVMCNCSGRADECVFDAEQYLSTSSG-----GRCIGCRDNTAGPHCEHCRDNFY-RPSPLLPCYNCSCNTMGSASLQCD-EEGVCACRPSVMGLKCDVCKAGFHSLSPGGCRLCKCDERGSVGVCSTEDGRCNCKLSVEGNSCDRCRPGSFNLQQDNPDGCQTCFCYGHSL 489 HSP 10 Score: 56.6102 bits (135), Expect = 6.466e-7 Identity = 81/309 (26.21%), Postives = 114/309 (36.89%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPW------KPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYP---CTPCQCHGLGTIG------------SCVSN--GED------------------------IGDQKP------GDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCK-NGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLK 1714
C C + + C +++E V NC N G+ CE C F P +P CE CN N N V G C+ C +T G +CE+C KGF+ + L + L P C PC C+ GT G C+++ G D +G P G C C+ G EG CD+C +G+ + + C C C GS + C C+ C C+ N Y N + E C CY I +Q S L +K+ DL+
Sbjct: 765 CPCPDRS-SCVQILETKAVVCT-NCPANQRGTRCELCEDGFYGNPLGQDGVVRPCVRCE-CNGNIDPNSVGICDHVT---------------GHCLKCLGHTAGDHCERCQKGFF--GSALDSTLGPSQKCKPCDCYPAGTFGLSNECDPDTGRCMCLNHVTGRDCGQCQAGYFNLQPGVGCERCNCNPVGSSSPACHPITGQCTCRTGVEGKQCDICRMGFFGFSSRGCRACNCDPMGSSSMQCHE-NGTCPCRQGFVGYK-CDKCELNHYLNHVTHQCEECPVCYSL-IRDQVSKL---KVKLHDLE 1047
BLAST of Laminin subunit alpha-2 vs. Ensembl Cavefish
Match: lama5 (laminin, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-030131-9823]) HSP 1 Score: 330.487 bits (846), Expect = 7.555e-90 Identity = 197/532 (37.03%), Postives = 273/532 (51.32%), Query Frame = 2
Query: 188 LMENAKITSSATCGTGGP-----EIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLF-QRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYP----------NGECLP------CPCSIDGSVRTSFYRCKP-----PCNCKVHVNSDDLCKTCKNGYFNLDSANPE----GCEPCYCFGITNQC 1672
L E KIT++ATCG P +++C+LV CD CD+ + + HPI A D WWQSP +++ +Y VNVT+DL Q++ + Y+L+K +SPRP W+LERS+D G+ Y PW FFA + C F QR + D+++ CTT YS+ L +GEI++SL+NGR MN Y + L F A IR RFL+ TL LM K + ++ RYYYS+++I IGG+C+CN HA+ C+ N +C+C+HNTCG +C+ CCP FNQ PWKP CE CNC+ + +C Y+ V + S++ G GGGVC+ CQ +T GINCE+CI G+YR + C+ CQC T G+C + G C CK Y G +CD C+ GYE +P NGE P C C+ G+ S C+P C CK + C C G++ L+ E GC C G+T QC
Sbjct: 53 LAEGTKITATATCGEDEPGRAIQDLYCKLVGGPVSGDPSQTIQGQYCDTCDSADSNRAHPITNAI-DGTERWWQSPPLSRSTEYNQVNVTLDLGQLFHVAYVLVKFANSPRPDLWVLERSVDFGKTYQPWQFFASSKRDCIEQFGQRTIERI----------YRDNDVICTTEYSRIVPLENGEIVVSLVNGRPGAMNFSYSPV------LREFTKATNIRLRFLRTNTLLGHLMG-KALRDPTVTRRYYYSIKDISIGGRCVCNGHAEACNAKDPNDPYKLQCDCQHNTCGPSCDHCCPGFNQLPWKPATTYSANECEPCNCHGHAFDCYYDPEVDQRRASLDIEGEFRGGGVCMECQHHTTGINCERCIPGYYRSPDHPLESPQACSKCQCESEFTDGTC--------EDHTGRCYCKPNYTGEHCDSCAEGYENFPSCYRKSPHVLNGEARPAGEIINCECNAAGTEGNS---CRPDPQTRTCVCKPGFTGEH-CDVCAPGHYTLNCLRCECSGPGCLDGSCDGLTGQC 554 HSP 2 Score: 313.153 bits (801), Expect = 1.615e-84 Identity = 219/745 (29.40%), Postives = 336/745 (45.10%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIG-DQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKG------SLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYG-DAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS-CKRCKCNNNQES------CDPVTGECR-CSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPC-NCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKC 3184
+C+CK N G +C C + P C CNC + E + + + + + C+ CQ T G + L C C C G +DI D G C CK G CD C+ G+ YPN C PC C+ G+ + C CK +V C C G F+LD NP+GC C+CFG T++C + +I +++GW L +++ + + + ++ S + + + + +W AP YLG+ ++SY RY ++ + T++G S + PDVI+K L + + E ++ + E ++EE + LV ++ L I+A + + +++ L L E ++E C CP + G SC+ C GY R L+ G+C C C HS+ C G C +C HNTAG HCE C G+ G ++ + SC CPCP N + C+ +G C+RC G+YGNP S C+ C CN+N + C P+TGEC+ C ++T G C+LC G+YGDA+T K+C +C+C G+ S CD TG+C C+ CD C +G++ + C C +CE + EK C+ +G C C NG C +C GY++Y CKKC C CD G+C C + + + CD+C
Sbjct: 1445 GQCHCKPNVIGRDCSECATGYYGFP-----NCRPCNCGTRLCEPVTGECICPPRTLLPE---------CVQCQPQTFGCH-----------------PLVGCEMCNCSPPGV------TSQDINCDTNSGQCRCKTNVIGRQCDRCAPGFYGYPN--CRPCNCNEAGTEKNVCDSVTGQCLCKENVEGPR-CDQCSLGTFHLDPTNPKGCTKCFCFGATDRCRKSDKRRNEIINMEGWVLLRGDRQEVPVSVYLDQDLVEADLSDVPDVYQD---LHWHAPQTYLGDKVSSYSGYLRYQLRTQ---TMRGDALYLPSEVSRPDVILKGNQMTLVY-MEREYSSPEQPHEGVVHLVEGSFRHAQTGNVVSREELMMVLVALESLQIRALHAQSAQAVSLQAAVLEGAEHLPTGRHANNVEICLCPASYQGDSCQQCAPGYYRDTKGLFLGKCVPCNCNGHSDQCLDGTGVCVDCRHNTAGDHCEKCQGGFRGNNSVDGHAVSCTSCPCPMQVASNNFAIRCVEKPNQMQCLCMPGYAGPKCERCAPGFYGNPMVIGSTCQPCNCNDNSDPNMLFSDCHPITGECQSCMFDTAGPHCELCAPGYYGDAITAKNCTRCNCSPCGTAS--CDPHTGQCQCKPGVSGVHCDHCENGMFGFN-SCSGCHHCECTAAAALEKSCDPVTGSCKCQPGANGPNCRQCAPGYWDYSPTGCKKCDCK----GGRCDPRTGECHCPAGLTGKQCDRC 2135 HSP 3 Score: 213.386 bits (542), Expect = 2.307e-54 Identity = 176/578 (30.45%), Postives = 244/578 (42.21%), Query Frame = 2
Query: 2234 PHIEKCNCPPGFNGLSCESCESGYRRV----NNVLYQGQCEACACYNHSNTC----------DSLN--------GECQNCTHNTAGAHCEDCASGYY--GDAKSKNLNSCKKCPCPRLDYQMTE-LCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKR------------------CKCN---NNQESC--DPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY---LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC--YNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETI--GCKKCNCYINGSYSNI--CNKISGICACKTAFFGSKCDRCNEGYYG-----FPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSD 3775
P+ +C+C G SC+ C G+ ++ +CE C C+ H+ C SL+ G C C H+T G +CE C GYY D ++ +C KC C + + T+ C ++ Y C N +G HCD C EGY P SC R C+CN SC DP T C C G C +C G Y T +C +C+C G +CD LTG+C CR+ F CD CT G + L ++C+ + G + C+ T G+C C G RCE+C G +YPNCK+C C P ++++ C + G C C N CD+C Y C PC C +GS +CD TG C C NV + C C G + + C P Q+ G C C P G CNKC W+ + GC C C G+ S + C + +G C CK C C +GY+ + CQ C C G+ C + G+C+C+ NV G KC R + D
Sbjct: 313 PYKLQCDCQHNTCGPSCDHCCPGFNQLPWKPATTYSANECEPCNCHGHAFDCYYDPEVDQRRASLDIEGEFRGGGVCMECQHHTTGINCERCIPGYYRSPDHPLESPQACSKCQC---ESEFTDGTCEDHTGRCY----CKPNYTGEHCDSCAEGYENFP----SCYRKSPHVLNGEARPAGEIINCECNAAGTEGNSCRPDPQTRTCVCKPGFTGEHCDVCAPGHY----TLNCLRCECSGPGCLDGSCDGLTGQCVCRSGFQGYSCDQCTPGYFNYPLCQRECV-----VLGTVPGG--CDPT-GRCVCRPEFTGPRCEQCSSGLHSYPNCKECTCDPRTSVNSSCSVA-GHCHCRPNYSGSKCDQCAPGFY--GFPSCSPCQCSLKGSRFTECDRVTGQCVCLPNVVGQHCDTCSPGSYSFPLCQADSCDPAGSVQNQILPTVGSCECKPYVEGVACNKCKPLYWNLSPDTLYGCSNCECNTGGTLSEVAECAQSNGQCYCKPNVCSGTCAVCKDGYFNLQKNNYFGCQGCQCDIGGSAG---LSCGERNGRCRCRPNVEGPKCNQPRPDHYFPD 861 HSP 4 Score: 200.675 bits (509), Expect = 1.614e-50 Identity = 162/543 (29.83%), Postives = 230/543 (42.36%), Query Frame = 2
Query: 2348 CACYNHSNTC------DSLNGECQNCTHNTAGAHCEDCASGY----YGDAKSKNLNSCKKCPC----------PRLDYQMTEL-CSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKI----SCKRCKCNN--NQESCDPVTGECRCSYNTMGTECQLCKHGFY--------------GDALTKS-CKKCDCDSLGSTSENC--DKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCEN-LHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQV---CNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
C C H+ C D +C +C HNT G C+ C G+ + A + + N C+ C C P +D + L VC +C H+T+G++C+RCI GYY +P + +C +C+C + +C+ TG C C N G C C G+ G+A C+C++ G+ +C D T C C+ F CD C G Y + C+ C C G + C+ +GQC C G C++C GYFNYP C++ + CD G+C C C++C + LH + N C C C R S+N C + G+C C N + C +C G++G C C G C+ G CVC PN +G C+ CS S+ F C+ +C GS N G C CK G C++C Y+ C C C GT S E+ +C + GQC CK NV C VC+ G F GC C C
Sbjct: 296 CVCNGHAEACNAKDPNDPYKLQC-DCQHNTCGPSCDHCCPGFNQLPWKPATTYSANECEPCNCHGHAFDCYYDPEVDQRRASLDIEGEFRGGGVCMECQHHTTGINCERCIPGYYRSPDHPLESPQACSKCQCESEFTDGTCEDHTGRCYCKPNYTGEHCDSCAEGYENFPSCYRKSPHVLNGEARPAGEIINCECNAAGTEGNSCRPDPQTRTCVCKPGFTGEHCDVCAPGHYTLN--CLRCECSGPGCLDGS--CDGLTGQCVCRSGFQGYSCDQCTPGYFNYPLCQRECVVLGTVPGGCD-PTGRCVCRPEFTGPRCEQCSSGLHSYPN---CKECTCDPRTSVNSSCSV-AGHCHCRPNYSGSKCDQCAPGFYGF--PSCSPCQCSLKGSRFTECDRVTGQCVCLPNVVGQHCDTCSPGSYSFPL---CQADSCDPAGSVQNQILPTVGSCECKPYVEGVACNKCKPLYWNLSPDTLYGCSNCECNTGGTLS-EVAECAQSNGQCYCKPNVCSGTCAVCKDGYFNLQKNNYFGCQGCQC 822 HSP 5 Score: 161.384 bits (407), Expect = 1.208e-38 Identity = 91/217 (41.94%), Postives = 121/217 (55.76%), Query Frame = 2
Query: 3197 YWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNG-QVCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNI--CNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKC 3832
++NN +PC C G+ + C G C C NV RDCS+C GY+G C PC G ++C G C+CPP T+ C +C ++ + +GC+ CNC G S C+ SG C CKT G +CDRC G+YG+PNC+PC+C E GT+ N CD +TGQC CK+NV G +C C GTF D T GC CFCFG +++C
Sbjct: 1420 FFNN--GALPCGCHEAGAESDMCQPFGGQCHCKPNVIGRDCSECATGYYGF-----PNCRPCNCGTRLCEPVTGECICPPRTLLPECVQCQPQTFGCHPLVGCEMCNCSPPGVTSQDINCDTNSGQCRCKTNVIGRQCDRCAPGFYGYPNCRPCNCNEAGTEKN---VCDSVTGQCLCKENVEGPRCDQCSLGTFHLDPTNPKGCTKCFCFGATDRC 1626 HSP 6 Score: 135.191 bits (339), Expect = 1.277e-30 Identity = 89/252 (35.32%), Postives = 120/252 (47.62%), Query Frame = 2
Query: 2228 EMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNT------CDSLNGECQNCTHNTAGAHCEDCASGYYGDA-KSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCE 2953
E P+ +C C PG+ G CE C G+ N ++ C+ C C ++S+ C + GECQ+C +TAG HCE CA GYYGDA +KN C PC T C ++ + C +C SGVHCD C G +G C C+C ++SCDPVTG C+C G C+ C G++ D CKKCDC CD TG+C C +QCD C + + +CE
Sbjct: 1919 EKPNQMQCLCMPGYAGPKCERCAPGFYG-NPMVIGSTCQPCNCNDNSDPNMLFSDCHPITGECQSCMFDTAGPHCELCAPGYYGDAITAKNCTRCNCSPC------GTASCDPHTGQ---C-QCKPGVSGVHCDHCENGMFGF-NSCSGCHHCECTAAAALEKSCDPVTGSCKCQPGANGPNCRQCAPGYW-DYSPTGCKKCDCKG-----GRCDPRTGECHCPAGLTGKQCDRCAEKYSVPVTRGADVHCE 2152 HSP 7 Score: 125.176 bits (313), Expect = 1.109e-27 Identity = 100/335 (29.85%), Postives = 139/335 (41.49%), Query Frame = 2
Query: 2711 CRCSYNTMGTECQLCKHGFYGDA---LTKSCKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKDK------KCIPCNCEIQGRISSEKFC-----NTTSGQCFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCS----PHALSADCDLNNGKCK-CGSNVINRICDKCENLHYWNNRT--DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV-TGCKKCPPCYNGQVCNNE-----GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGY 3616
C C + G CQ C G+Y D C C+C+ S+ C TG C DCR N C+ C G + C C C +Q ++S F QC C+ G +CE+C G++ P C+ C C+ P+ L +DC G+C+ C + C+ C +Y + T +C CNC CD TG C+C V+ C C+ G FG + +GC C C G C C P G C +C+ WD++ T GCKKC+C C+ +G C C G +CDRC E Y
Sbjct: 1818 CLCPASYQGDSCQQCAPGYYRDTKGLFLGKCVPCNCNG---HSDQCLDGTGVCVDCRHNTAGDHCEKCQGGFRGNNSVDGHAVSCTSCPCPMQ--VASNNFAIRCVEKPNQMQCLCMPGYAGPKCERCAPGFYGNPMVIGSTCQPCNCNDNSDPNMLFSDCHPITGECQSCMFDTAGPHCELCAPGYYGDAITAKNCTRCNCSPCG-TASCDPHTGQCQCKPGVSGVHCDHCENGMFGFNSCSGCHHCE-CTAAAALEKSCDPVTGSCKCQPGANGPNCRQCAPGYWDYSPT-GCKKCDCK-----GGRCDPRTGECHCPAGLTGKQCDRCAEKY 2139 HSP 8 Score: 115.161 bits (287), Expect = 1.158e-24 Identity = 70/201 (34.83%), Postives = 97/201 (48.26%), Query Frame = 2
Query: 2801 CDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN-YP--NCKKCLCSPHALSA---DCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY 3382
C C G+ S+ C G+C C+ N + R C C +G Y C PCNC + C +G+C C C +C F +P C+ C CSP +++ +CD N+G+C+C +NVI R CD+C Y +C PCNC G+ CD TG C C +NV C +C +G F LD T K C C+
Sbjct: 1428 CGCHEAGAESDMCQPFGGQCHCKPNVIGRDCSECATG-YYGFPNCRPCNC-------GTRLCEPVTGECICPPRTLLPECVQCQPQTFGCHPLVGCEMCNCSPPGVTSQDINCDTNSGQCRCKTNVIGRQCDRCAPGFY--GYPNCRPCNCNEAGTEKNVCDSVTGQCLCKENVEGPRCDQCSLGTFHLDPTNPKGCTKCF 1618 HSP 9 Score: 114.775 bits (286), Expect = 1.704e-24 Identity = 93/331 (28.10%), Postives = 127/331 (38.37%), Query Frame = 2
Query: 2858 CDCRTNFVNRQCDSCTSGLYLKDK-----KCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGY-------FNYPNCKKCLCSPHALSADCDL------NNGKCKCGSNVINRICDKCENLHYWNNR---TDCVPCNCGRGS----LNPKCDMETGNCK-CAQNVARRDCSKCDVGYFGLDVTG--CKKC--PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
C C ++ C C G Y K KC+PCNC C +G C C N GD CEKC G+ + +C C C S + + N +C C C++C Y N + C PCNC S L C TG C+ C + A C C GY+G +T C +C PC + G C C P G C+ C + FN GC C C + C+ ++G C C+ G C +C GY+ + C+ C C +CD TG+C C + G +C C
Sbjct: 1818 CLCPASYQGDSCQQCAPGYYRDTKGLFLGKCVPCNCN-----GHSDQCLDGTGVCVDCRHNTAGDHCEKCQGGFRGNNSVDGHAVSCTSCPCPMQVASNNFAIRCVEKPNQMQCLCMPGYAGPKCERCAPGFYGNPMVIGSTCQPCNCNDNSDPNMLFSDCHPITGECQSCMFDTAGPHCELCAPGYYGDAITAKNCTRCNCSPCGTASCDPHTGQCQCKPGVSGVHCDHCENGMFGFNSCSGCHHCECTAAAALEKSCDPVTGSCKCQPGANGPNCRQCAPGYWDYSPTGCKKCDCKGG--------RCDPRTGECHCPAGLTGKQCDRC 2135 HSP 10 Score: 114.005 bits (284), Expect = 2.657e-24 Identity = 83/265 (31.32%), Postives = 125/265 (47.17%), Query Frame = 2
Query: 4313 LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAI--GKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN---DKNYDQCEPCQCPS--AHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQQGSQSL---NCNEF-GICQ-CSPGVLGIKCDQCAPNYVVEKKTCQSC 5068
++R+ MM L+ + SL+++ ++ + + V+E + + G+ VE C+C A G+ CQ GYY++ T L CNCNG+S C TG C +C N G+ C+ C+ GF N D + C C CP A NFA C +E +++C C GY+G CE C GFYGNP + G C+ C+CN ++ +C+ G CQ C G C+ CAP Y + T ++C
Sbjct: 1765 VSREELMMVLVALESLQIR----ALHAQSAQAVSLQAAVLEGAEHLPTGRHANNVEICLCPASYQGDSCQQCAPGYYRD--------------TKGLFLGKCVPCNCNGHSDQCLDGTGVCVDCRHNTAGDHCEKCQGGFRGNNSVDGHAVSCTSCPCPMQVASNNFAIRC--VEKPNQMQCLCMPGYAGPKCERCAPGFYGNPMVIGSTCQPCNCNDNSDPNMLFSDCHPITGECQSCMFDTAGPHCELCAPGYYGDAITAKNC 2009 HSP 11 Score: 99.3673 bits (246), Expect = 8.550e-20 Identity = 144/676 (21.30%), Postives = 254/676 (37.57%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINL--KKCNCNGYSKV------CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKT--------------------CQSCYSG-----CTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSIN-------KFYKLKNEIKNFSI-QFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINE--TISGHEQLGISNELI---KNATAYLETIKNIYKEMPNI-----TLAASVLKDVLKYVDELNQIIKYTQSHIQSDQIN------NLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIIL---INERMKDLQKIFRKMT---KQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSN-----HLVSKSLIIPDTLKKHVE------------QQLNKSSNLIKKLTKDFN----------FLHNAEQAKEAYSNITRDILAVDYAINQTKKE----------LDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNI 6196
+C+C G C+ G+Y GN ++I + CNCN S C +TG+CQ+CM + G C+LC G+Y + C C C T +CQC+ G SG C++C+ G +G N G C C+C + +C+ G C+C PG G C QCAP Y T C + +G C + V + T V + + L L+K+N + + L +I+ +F+ L I+N SI + D +++K +E K L+ + S ++ E ++S + + N I + A L+T+KN K++ T+ LK L V + + +++ + Q Q + N + + K+ MAD N + K H+ L +NE + K KQ D ++K+ L K D + +V ++ D++ L + L ++ L + V+ ++ K + L+ +L K+ + F+ A A AY+NI + D + K L++ + +K L + KS+++ +++ +
Sbjct: 1925 QCLCMPGYAGPKCERCAPGFY-----------------GNPMVIGSTCQPCNCNDNSDPNMLFSDCHPITGECQSCMFDTAGPHCELCAPGYYGDAITAKNCTRCNCSPCGTASCDPHTG-------QCQCKPGVSGVHCDHCENGMFGFNSCSG--CHHCECTAAAALEKSCDPVTGSCKCQPGANGPNCRQCAPGYWDYSPTGCKKCDCKGGRCDPRTGECHCPAGLTGKQCDRCAEKYSVPV-TRGADVHCERCDSCVVELLRDLDKVNGTYLSAEHQLKNISTTAFAWAQFHNLNKSIENKSINNYNSSLDGSRDKANTLE-------KELQTIDSDLFELQEKASLSSRKGATLENNTIATKERAQNLLDTVKNYMKDIEAAQNGSQTMDEEELKRKLAEVAAMLRDMRFRSTTPQGKQADYELSQANQLLDRVKSEMADRTAANEATVEEIKNKLNKFHQELTSLRDALNEAANNTALASEKNNANQKQLDENQQKVNDLKKKQKEVTDTMQMAEDDVAQVNDMLAMLQDSKEEYERLAAQLDGARQPLAEKVQNFSTSGAKSPLVEEAEKHAELLDQLAKNLSSVISDTNQDGFIQRALNASRAYNNIINSVNEADNLAKEANKSATEALEPKCVLNWTELKPQLQDIKNRLQNATTKKSDLMMDLQKV 2566 HSP 12 Score: 97.8265 bits (242), Expect = 2.011e-19 Identity = 57/168 (33.93%), Postives = 83/168 (49.40%), Query Frame = 2
Query: 2690 CDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKC 3184
C P G+C C N +G +C C G+YG +C+ C+C + C+ +TG+C C + +C C + C CNC G S + C+T SGQC C NV G +C++C G++ YPNC+ C C+ + CD G+C C NV CD+C
Sbjct: 1440 CQPFGGQCHCKPNVIGRDCSECATGYYG---FPNCRPCNCG-----TRLCEPVTGECICPPRTLLPECVQCQPQTFGCHPLVGCEMCNCSPPGVTSQDINCDTNSGQCRCKTNVIGRQCDRCAPGFYGYPNCRPCNCNEAGTEKNVCDSVTGQCLCKENVEGPRCDQC 1599 HSP 13 Score: 70.0922 bits (170), Expect = 5.803e-11 Identity = 78/331 (23.56%), Postives = 127/331 (38.37%), Query Frame = 2
Query: 686 YSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPG--DCICKKGYEGVNCDLCSIGYERYP---NGECLPCPCSIDGSVRTSFYRCKP---PCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
YS P+ + G ++ L+ G S + + ++ S + ++ ++ ++ R L H A + LE + + N+EI C C + G +C+ C P + + C CNCN S++C ++G GVC++C+ NT G +CEKC GF R N + CT C C + +N +KP C+C GY G C+ C+ G+ P C PC C+ + F C P C + + C+ C GY+ D+ + C C C
Sbjct: 1738 YSSPEQPHEG--VVHLVEG--SFRHAQTGNVVSREELMMVLVALESLQIRAL-HAQSAQAVSLQAAVLEGAEHLPTGRHANNVEI-------------------------CLCPASYQGDSCQQCAPGYYRDTKGLFLGKCVPCNCNGHSDQC------------------LDGTGVCVDCRHNTAGDHCEKCQGGF-RGNNSVDGHAVSCTSCPC----PMQVASNNFAIRCVEKPNQMQCLCMPGYAGPKCERCAPGFYGNPMVIGSTCQPCNCNDNSDPNMLFSDCHPITGECQSCMFDTAGPHCELCAPGYYG-DAITAKNCTRCNC 2014 HSP 14 Score: 67.0106 bits (162), Expect = 4.338e-10 Identity = 49/147 (33.33%), Postives = 69/147 (46.94%), Query Frame = 2
Query: 4607 NLKKCNCNGYSKVCDS--VTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGS-QSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
N K+C C+ + V S V G C +C N G+KC C GFY + C PCQC F + C R+ +C C G+ C+ C G Y P+ C++ C+ GS Q+ G C+C P V G+ C++C P Y
Sbjct: 621 NCKECTCDPRTSVNSSCSVAGHC-HCRPNYSGSKCDQCAPGFY----GFPSCSPCQCSLKGSRFTE-CDRVTG----QCVCLPNVVGQHCDTCSPGSYSFPL----CQADSCDPAGSVQNQILPTVGSCECKPYVEGVACNKCKPLY 753 HSP 15 Score: 64.3142 bits (155), Expect = 3.188e-9 Identity = 52/195 (26.67%), Postives = 75/195 (38.46%), Query Frame = 2
Query: 4478 CICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNG---YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIES-----SLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
C+C G HC G+Y +N +C C+G CD +TG C C +G C C G++ NY C+ + C + + +C CR ++G CE C G + P CK C C+ + S + +C+ G C C P G KCDQCAP +
Sbjct: 509 CVCKPGFTGEHCDVCAPGHYT---------------------LNCLRCECSGPGCLDGSCDGLTGQCV-CRSGFQGYSCDQCTPGYF----NYPLCQ------------RECVVLGTVPGGCDPTGRCVCRPEFTGPRCEQCSSGLHSYPN----CKECTCDPRTSVNSSCSVAGHCHCRPNYSGSKCDQCAPGF 661 HSP 16 Score: 63.5438 bits (153), Expect = 4.889e-9 Identity = 46/131 (35.11%), Postives = 60/131 (45.80%), Query Frame = 2
Query: 4646 CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAP 5032
CD TG C C G +C+ C G + +Y C+ C C P N + S+ C CR YSG C+ C GFYG P C C C+ +GS+ C+ G C C P V+G CD C+P
Sbjct: 592 CDP-TGRCV-CRPEFTGPRCEQCSSGLH----SYPNCKECTCDPRTSVNSS-------CSVAGHCHCRPNYSGSKCDQCAPGFYGFP----SCSPCQCSLKGSRFTECDRVTGQCVCLPNVVGQHCDTCSP 705 HSP 17 Score: 62.003 bits (149), Expect = 1.541e-8 Identity = 78/311 (25.08%), Postives = 111/311 (35.69%), Query Frame = 2
Query: 911 YYYSLRNIEIGGKCI---CNNHADKCDKVIENGISVAKCNCKHNTCGSNCETC---------------------CPL-------------------------------------FNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPN-GECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFG 1657
YY + + +G KC+ CN H+D+C + G+ V +C+HNT G +CE C CP+ F P + C+ CNCN S+ + G C +C +T G +CE C G+Y A CT C C GT SC D G C CK G GV+CD C G + + C C C+ ++ S C C+ N + C+ C GY++ +P GC+ C C G
Sbjct: 1836 YYRDTKGLFLG-KCVPCNCNGHSDQC--LDGTGVCV---DCRHNTAGDHCEKCQGGFRGNNSVDGHAVSCTSCPCPMQVASNNFAIRCVEKPNQMQCLCMPGYAGPKCERCAPGFYGNPMVIGSTCQPCNCNDNSDPNMLFSDCHPIT------------GECQSCMFDTAGPHCELCAPGYYGD----AITAKNCTRCNCSPCGT-ASC--------DPHTGQCQCKPGVSGVHCDHCENGMFGFNSCSGCHHCECTAAAALEKSCDPVTGSCKCQPGANGPN-CRQCAPGYWDY---SPTGCKKCDCKG 2111 HSP 18 Score: 61.2326 bits (147), Expect = 2.818e-8 Identity = 78/334 (23.35%), Postives = 139/334 (41.62%), Query Frame = 2
Query: 4586 TGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYV----VEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSEL----LNSINKFYK----------LKNEIKNFS------IQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQL--GISNELIKNATAYLETIKNIYKEMPNITLAA 5503
TG N + CNC +++C+ VTG+C C +C C+ + CE C C P + NC +C+C+ G+ C+ C GFYG P C+ C+CN+ G++ C+ G C C V G +CDQC+ K C C+ D + D +IN + L L ++ +S+ + +L L+ + Y+ L +++ ++S ++ Q M+ DA E+ +R + +L+ + T + S EQ G+ + L++ + + +T + +E + L A
Sbjct: 1463 TGYYGFPNCRPCNCG--TRLCEPVTGECI-CPPRTLLPECVQCQPQTFGCHPLVG-CEMCNCSPPGVTSQDINCDTNSG----QCRCKTNVIGRQCDRCAPGFYGYPN----CRPCNCNEAGTEKNVCDSVTGQCLCKENVEGPRCDQCSLGTFHLDPTNPKGCTKCFCFGATDRCRKSDKRRNEIINMEGWVL---LRGDRQEVPVSVYLDQDLVEADLSDVPDVYQDLHWHAPQTYLGDKVSSYSGYLRYQLRTQTMRGDALYLPSEV----SRPDVILKGNQMTLVYMEREYSSPEQPHEGVVH-LVEGSFRHAQTGNVVSREELMMVLVA 1776 HSP 19 Score: 54.6842 bits (130), Expect = 2.714e-6 Identity = 57/213 (26.76%), Postives = 80/213 (37.56%), Query Frame = 2
Query: 1007 VAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEP 1642
+C C+ G +C+ C P + P C R EC TV G + G C+ C+ G CE+C G + YP C C C ++ S S G C C+ Y G CD C+ G+ +P+ C PC CS+ GS T R C C +V C TC G ++ + C+P
Sbjct: 551 TGQCVCRSGFQGYSCDQCTPGYFNYPL----------CQR---ECVVLGTVP---------GGCDPTGRCV-CRPEFTGPRCEQCSSGLHS---------YPNCKECTCDPRTSVNSSCSVA--------GHCHCRPNYSGSKCDQCAPGFYGFPS--CSPCQCSLKGSRFTECDRVTGQCVCLPNVVGQH-CDTCSPGSYSFPLCQADSCDP 720 HSP 20 Score: 53.5286 bits (127), Expect = 5.487e-6 Identity = 63/221 (28.51%), Postives = 85/221 (38.46%), Query Frame = 2
Query: 1007 VAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLC-SIGYERYPNG--ECLPCPCSIDGSVRTSFYRCKPP---CNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
C+C+ N GS C+ C P F P C C C+ K + + R+ G VC+ N G +C+ C G Y A P Q L T+GSC CK EGV C+ C + + P+ C C C+ G++ + C C CK +V S C CK+GYFNL N GC+ C C
Sbjct: 640 AGHCHCRPNYSGSKCDQCAPGFYGFP-----SCSPCQCSLKGSR-------------FTECDRVTGQCVCL---PNVVGQHCDTCSPGSYSFPLCQADSCDPAGSVQNQILPTVGSCE---------------CKPYVEGVACNKCKPLYWNLSPDTLYGCSNCECNTGGTL-SEVAECAQSNGQCYCKPNVCSG-TCAVCKDGYFNLQKNNYFGCQGCQC 822
BLAST of Laminin subunit alpha-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008925.1 (pep scaffold:Pmarinus_7.0:GL476496:30277:70426:-1 gene:ENSPMAG00000008054.1 transcript:ENSPMAT00000008925.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 619.387 bits (1596), Expect = 0.000e+0 Identity = 374/1092 (34.25%), Postives = 541/1092 (49.54%), Query Frame = 2
Query: 455 QVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMT--HKGYLEQS--IYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQK----PGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQ-STLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVF----IYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELL---NITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGEC----QNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPR--LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRCKCNNNQES------CDPVTGEC-RCSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYW-NNRTDCVPCNCGRG-SLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDV----------TGCKKCPPCYNGQVCN---NEGICVCPPNTIGNLCNKCSEDSWDFN--ETIGCKKCNCYINGSYSNICNKISGI 3559
QV+Q+ Y+++K +SPRPGNWILERSLDG Y PW +++ + C + T P DDE+ CT+YY L G+I SLINGR S + L++F SARYIR R + TL ADLMT H+ E + RYYYS+++I +GG CIC+ HA C +C C+HNTCG NC+ CCP +NQR W+P + CE+CNC+ K+ +C Y+ V S++ GR GGGVC+NC NT G NCE C GFYRP VL + YPC CQC G++ E D + PG C+C+ G+ G C+ C+ Y YP +C PC CS+ G T+ C PC CK V + C C+ G+++L NP GC C+CFG+++ C SL ++ D++GW++T G + + F + N+ D R P +YYW AP YLGN +T+Y RY V ++ + +I DVII+ G+ + S S+ L Q F + ++ + +++ ++ L V +L I+A Y S ++++ L ++ ++ + + +E+C+CP G+ G SCESC G+RRV+ L+ G+CE C C+ H++ CD L+G+C C HNT G HCE C G+YG+A + C C CP + C C +CL G+ C+RC + ++G P SC+RC+CN N+E+ CD TG+C RC T G C+ C GFYGDA T C+ C C G+T+ CD++ G+C CR + V R C+ C G Y C PC C + + + G+C C V G C++C G++ + C C CS + CD G+C C N C +C + H+ N C CNC S +CD +G C C R C +C G +G T CP G +C G C C N G C+ C ++ N + GC C C+ S++C ++ G
Sbjct: 1 QVFQVAYVIVKAANSPRPGNWILERSLDGATYHPWQYYSVSDSECLTRYG------ITPTVGNPVYRRDDEVICTSYYVVLLMLARGKIHTSLINGRPSADDPS--------PKLLDFTSARYIRLRLQRIRTLNADLMTLSHRDPREVDPIVTRRYYYSIKDISVGGMCICSGHASTCP--WNEDTQKMECQCEHNTCGENCQHCCPGYNQRRWRPGTINNGNTCEKCNCHGKTEDCYYDAEVDRTNRSLSVHGRFSGGGVCVNCSANTAGTNCETCRDGFYRPTGVLPNDPYPCRLCQCDPQGSLSQVCIKDEKHADPEKDLSPGQCLCRPGFAGERCERCAFAYRGYP--DCKPCLCSMAGG--TNDDPCSEPCVCKERVEGEH-CDRCRAGFYDLRPRNPRGCSACFCFGLSSSCRSLPWGVTQVVDMRGWRVTDRQGLR-KVKTFVEVDQVAVRNA--DVRRTLPALYYWLAPTSYLGNKLTAYAGHLRYSVSYDIPVDSTDSEMISDVDVIIE-GNGQALSSGSLG---LMLQPFEEQTLSLRLLPENFFDFRSNAPVSRDALMTALANVTRLQIRASYSSVKQAVYRLSAVSLDVASPDAAVGSPAALDVEQCHCPHGYAGTSCESCMRGHRRVDGTLHGGRCEPCRCHGHADDCDDLSGDCMLPLSGCRHNTMGPHCELCRPGFYGNATRGTADDCLPCTCPLSIASNNFSPTCHQDPRGVLTCDQCLPGYIGLRCERCADDFFGEPSSPGGSCRRCECNGNEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGGCQPCQCHPEGATAPQCDRINGQCPCRPSVVGRTCEQCAIGFYGLSSGAGCSPCPCHP---VGTAGVACSADGRCHCWPGVEGRSCDRCTSGHYGFKEGGCTPCNCS--HTNHHCDQETGRCLCPPNTEGTRCHRCIDDHWGVNPHAGCRACNCSAAHSRGARCDEASGQCSCLDGYGGRTCGECAQGRWGYPACRPCECHPEGTRANTCPAPPTGSLCGCDERTGQCACKENVGGTRCDACLPGTFGLNREDPRGCTACFCF---GVSSVCRELQGF 1056 HSP 2 Score: 241.891 bits (616), Expect = 1.999e-64 Identity = 189/700 (27.00%), Postives = 298/700 (42.57%), Query Frame = 2
Query: 2567 CHKCLHNTSGVHCDRCIEGY--YGNPKKKISCKRCKCNNNQESCDPVTGECR-----CSYNTMGTECQLCKHGFYGDALTKS---CKKCDC------DSLGSTSENCDKLTGKCD-CRTNFVNRQCDSCTSGLYLKDKK----CIPCNCEI-QGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPHALSA-DCDLNNGKCKCGSNVINRICDKCE-NLHYWNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSN--------ELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSL----ESGKDYSTIHLNANYPRRFGWPLTMFR-----PIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNG-LFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKL-YTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQ 4516
CH C H +G C+ C+ G+ C+ C+C+ + + CD ++G+C C +NTMG C+LC+ GFYG+A + C C C ++ T + CD C ++ +C+ C + + C C C + + C+ +GQC C E G CE+C +G++ C+ C C P +A CD NG+C C +V+ R C++C + ++ C PC C G+ C + G C C V R C +C G++G GC C + C+ E G C+CPPNT G C++C +D W N GC+ CNC S C++ SG C+C + G C C +G +G+P C+PC C GT++N L CD+ TGQC CK+NV GT+C C GTF + GC +CFCFGVS C + V ++ + + + S+ + G+ + + +P + + P Y + F L+ + + V + D GR E + N L R + +D EH +W +Y N +LD+ + +R FM L + ++ +K Y ++ I + + + VE+C C G CQ
Sbjct: 592 CH-CPHGYAGTSCESCMRGHRRVDGTLHGGRCEPCRCHGHADDCDDLSGDCMLPLSGCRHNTMGPHCELCRPGFYGNATRGTADDCLPCTCPLSIASNNFSPTCHQDPRGVLTCDQCLPGYIGLRCERCADDFFGEPSSPGGSCRRCECNGNEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGGCQPCQCHPEGATAPQCDRINGQCPCRPSVVGRTCEQCAIGFYGLSSGAGCSPCPCHPVGTAGVACSAD-GRCHCWPGVEGRSCDRCTSGHYGFKEGGCTPCNCSHTNHHCDQETGRCLCPPNTEGTRCHRCIDDHWGVNPHAGCRACNCSAAHSRGARCDEASGQCSCLDGYGGRTCGECAQGRWGYPACRPCECHPEGTRANTCPAPPTGSLCGCDERTGQCACKENVGGTRCDACLPGTFGLNREDPRGCTACFCFGVSSVCRELQG--FVRMQVF---MVEGQRSMPVVNQVGQRETMSGVRYQHPEMILHAGEVLKTLHHEPFYWKLPSQFTGPKLTAYGGKLRYTVYFEAEDGS-GRSDREPQVLLRGGRNKELLIYRDMAPPRPGQRTQHQMDMTEH----EW---RYFNSVLDQPV------------SRADFMSILGGIGNIFIKASYGSRMTESRISEVSLEVAARGNGSSHLQAACQVEQCECPPGYSGLSCQ 1264 HSP 3 Score: 165.236 bits (417), Expect = 8.381e-41 Identity = 147/591 (24.87%), Postives = 234/591 (39.59%), Query Frame = 2
Query: 3407 GICV-CPPNTIGNLCNKCSEDSWDF-----NETIGCKKCNCYINGSYSNICNK-----------ISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSN--LTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTA-VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDC----QNCMGNREGNKCQLCREGFYKNDK--NYDQCEPCQCPS--AHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIK-GIPCKSCDCNQQ-----GSQSLNCNEFGICQCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
G+CV C NT G C C + + N+ C+ C C GS S +C K G C C+ F G +C+RC Y G+P+C+PC C+ G ++ D + C CK+ V G C CR G + GC +CFCFG+S C ++ + + + D + L K + + ++ R T+ Y + + L+ + + +VS D + E+ +V +E S + ++ ++ L ++ E+ SN ++RD M AL NVT L+++ V + ++ ++ V A+G VE+C C G C++ + G+ + G++ G + C C+G++ CD ++GDC C N G C+LCR GFY N D C PC CP A NF+ C + + QC GY G CE C F+G P G C+ C+CN G + + +C G C++CA + CQ C
Sbjct: 245 GVCVNCSANTAGTNCETCRDGFYRPTGVLPNDPYPCRLCQCDPQGSLSQVCIKDEKHADPEKDLSPGQCLCRPGFAGERCERCAFAYRGYPDCKPCLCSMAGGTND-----DPCSEPCVCKERVEGEHCDRCRAGFYDLRPRNPRGCSACFCFGLSSSCRSLPWGVTQVVDMRGWRVTD-RQGLRKVKTF--VEVDQVAVRNADVRRTLPALYYWLAPTSYLGNKLTAYAGHLRYSVSYDIPVDSTDSEMISDVDVIIEGNGQALS----------------------SGSLGLMLQPFEEQTLSLRLLPENFFDFRSNAPVSRDALMTALANVTRLQIRASYSSVKQAVYRLSAVSLDVASPDAAVGSPAALDVEQCHCPHGYAGTSCESCMRGHRR---------VDGTLHGGR-----CEPCRCHGHADDCDDLSGDCMLPLSGCRHNTMGPHCELCRPGFYGNATRGTADDCLPCTCPLSIASNNFSPTCHQDPRGVLTCDQCLPGYIGLRCERCADDFFGEPSSPGGSCRRCECNGNEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGGCQPC 791 HSP 4 Score: 124.405 bits (311), Expect = 2.967e-28 Identity = 97/386 (25.13%), Postives = 157/386 (40.67%), Query Frame = 2
Query: 1208 GVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPP--------------CNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSA----KNLKISDLKGWKLTTFTGEKIALP--KFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCG---DFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITL---SRGEVHLALDEMPHIEKCNCPPGFNGLSCE 2287
G C+ C NTEG C +CI W V C C C + G+ D+ G C C GY G C C+ G YP C PC C +G T C P C CK +V C C G F L+ +P GC C+CFG+++ C L + + + + G++ + ++ + + + + P +YW P + G +T+YG RY V E + G P V+++ G + ++ ++ + Q + + EW+ D+ ++ +F+S L + + IKA Y S + ++L +RG L +E+C CPPG++GLSC+
Sbjct: 901 GRCL-CPPNTEGTRCHRCIDDH---WGV--NPHAGCRACNCSAAHSRGARC-------DEASGQCSCLDGYGGRTCGECAQGRWGYP--ACRPCECHPEG---TRANTCPAPPTGSLCGCDERTGQCACKENVGGT-RCDACLPGTFGLNREDPRGCTACFCFGVSSVCRELQGFVRMQVFMVEGQRSMPVVNQVGQRETMSGVRYQHPEMILHAGEVLKTLHHEP--FYWKLPSQFTGPKLTAYGGKLRYTVYFEAEDG-SGRSDREPQVLLRGGRNKELLIYRDMAPPRPGQRTQHQMDMTEHEWRYFNSVLDQPVSRADFMSILGGIGNIFIKASYGSRMTESRISEVSLEVAARGNGSSHLQAACQVEQCECPPGYSGLSCQ 1264 HSP 5 Score: 90.1225 bits (222), Expect = 8.349e-18 Identity = 56/159 (35.22%), Postives = 76/159 (47.80%), Query Frame = 2
Query: 4613 KKCNCNG------YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQC-APNYVVEKKTCQSC 5068
++C CNG +VCD+ TG C C G C+ C +GFY + C+PCQC A C RI +C CR G++CE C GFYG G C C C+ G+ + C+ G C C PGV G CD+C + +Y ++ C C
Sbjct: 735 RRCECNGNEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGGCQPCQC-HPEGATAPQCDRING----QCPCRPSVVGRTCEQCAIGFYGL-SSGAGCSPCPCHPVGTAGVACSADGRCHCWPGVEGRSCDRCTSGHYGFKEGGCTPC 887 HSP 6 Score: 65.855 bits (159), Expect = 1.911e-10 Identity = 77/322 (23.91%), Postives = 126/322 (39.13%), Query Frame = 2
Query: 4619 CNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNC------------NEFGICQCSPGVLGIKCDQCAPN-YVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVL-EDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYK--EMPNITLAASVLKDVLKYVD 5536
CNC+ + CD TG C C N EG +C C + + + + C C C +AH A+ C +C C GY G++C C QG +G P C+ C+C+ +G+++ C G C C V G +CD C P + + ++ + GCT + ++ + F +R+ + Q S ++N + + + + Q +M A GE+ LKT +++ L S FT T G + T Y E + P + L K++L Y D
Sbjct: 887 CNCSHTNHHCDQETGRCL-CPPNTEGTRCHRCIDDHWGVNP-HAGCRACNCSAAHSRGAR-CDEASG----QCSCLDGYGGRTCGECAQGRWGYPA----CRPCECHPEGTRANTCPAPPTGSLCGCDERTGQCACKENVGGTRCDACLPGTFGLNREDPR----GCTACFCFGVSSVCRELQGF----------VRMQVFMVEGQRSMPVVNQVGQ----RETMSGVRYQHPEMILHA----GEV--LKTLHHEPFYWKLPSQFTGPKLTAYGGK---------LRYTVYFEAEDGSGRSDREPQVLLRGGRNKELLIYRD 1164 HSP 7 Score: 63.5438 bits (153), Expect = 8.558e-10 Identity = 91/408 (22.30%), Postives = 154/408 (37.75%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCD------------------SVTGDCQNCMGNREGNKCQLCREGFYK------NDKNYDQCEPCQCPSAHYNFAQNCTRIESSLEL-------KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLN-CNEFGICQCSPGVLGIKCDQCAPNYVVEK----KTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISI-QMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRS-TFTDINETISGHEQ------LGISNELIKNATAYLETI-KNIYKEMPNITLAASVLKDVLKYVDELNQIIKYT 5563
+C C T G +CQ+ GY + + + G++ GN +KCNC+G ++ C S G C NC N G C+ CR+GFY+ ND C CQC + +Q C + E + +C CR G++G+ CE C + G P CK C C+ G + + C+E C C V G CD+C + + + C +C+ + V++ + + LR K + + Q++ + L + S K+ Y + Y+ ++ S +D++ I G+ Q LG+ + + T L + +N + N ++ L L V L Y+
Sbjct: 175 ECQCEHNTCGENCQHCCPGYNQRRW------RPGTINNGN----TCEKCNCHGKTEDCYYDAEVDRTNRSLSVHGRFSGGGVCVNCSANTAGTNCETCRDGFYRPTGVLPNDPY--PCRLCQC-DPQGSLSQVCIKDEKHADPEKDLSPGQCLCRPGFAGERCERCAFAYRGYPD----CKPCLCSMAGGTNDDPCSE--PCVCKERVEGEHCDRCRAGFYDLRPRNPRGCSACFCFGLSSSCRSLPWGVTQVVDMRGWRVTDRQGLRKVKTFVEVDQVAVRNADVRRTLPALYYWLAPTSYLGNKLT-----AYAGHLRYSVSYDIPVDSTDSEMISDVDVIIEGNGQALSSGSLGLMLQPFEEQTLSLRLLPENFFDFRSNAPVSRDALMTALANVTRLQIRASYS 558
BLAST of Laminin subunit alpha-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008872.1 (pep scaffold:Pmarinus_7.0:GL476733:325925:343343:-1 gene:ENSPMAG00000008023.1 transcript:ENSPMAT00000008872.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 343.969 bits (881), Expect = 3.176e-102 Identity = 239/710 (33.66%), Postives = 343/710 (48.31%), Query Frame = 2
Query: 1112 CNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWK-LTTFTGEKIALPKFT-SFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDR-RLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDAL-TKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP----NCKKCLC-SPHALSADCDLNNGKCKCGSNVINRICDKC 3184
CNC+ K+ EC Y+ VA LS+N G+ GGGVC+NC +NT G+NCE C+ ++RP PC PC C+ +G++ C++N C CK GY G C+ C+ GY+ +P +C+ C CS +GS+ CK PC CK +V C +CK G++NL +N GC C+CFG+T C S+ K+ +KI + T + L+ R PT YG + + +I + +G P V G+ H + R R PT P + L + + + A ++ S L + +A + +E C CPPG++G SCE+C G+RRVN LY G CE C C+ H+ CD ++G C NC HN+ G CE C G+YG+A+ + C+ C CP L + T C S +C +C +G CDRC +GYYG+P SC+ C+CN N + +CD VTGEC RC T G C+ C G++GDA+ K+C+ C C S GS S CD +TG+C+C+ N RQCD C + + +KC PC C ++G +SS C+ G+C C +G RCE ++ C C C S A S DCD +G+C C V+ R CD+C
Sbjct: 1 CNCHGKAGECYYDPDVARRNLSLNTAGKFVGGGVCLNCSQNTAGVNCEICVDEYFRP------PKNPCRPCNCNPVGSLSKVCINNSRS--------CRCKPGYGGERCNRCAFGYKGFP--KCVKCKCSTEGSINKD--PCKEPCICKSNVQGKH-CDSCKPGHYNLQESNALGCTECFCFGVTRMCDSIPWARAKVRGQAARHGADGRINKKIHVMDITLWWTRLSLAVQDTVSRGMMPTAQ-------------PDYGRGNKKMTRIHKTTYTRGPPPPPPCVSALVGEKLELHRCCLLPRASWARGPHHAPLPTPPSPCAPLPVPLCPHPSLHTPL-GLHRAPLPAPLSPHRLSSVTLEVAAEGTRGAVAASD---VELCQCPPGYSGTSCEACVRGHRRVNGSLYGGACEPCDCHGHATECDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCACP-LGFSPT--CRVGPSGAMLCDRCAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFGDAIVAKNCQPCKCSSNGSQSNTCDAVTGQCECKPNVAGRQCDHCVPNCWWDAQQRKCQPCRCSLEGAVSSA--CD-KHGRCACRPEFSGKRCEPSQARHYGMSQQPTGCAPCNCNSFGAFSFDCD-ESGQCHCQPGVVGRQCDRC 667 HSP 2 Score: 117.087 bits (292), Expect = 1.956e-26 Identity = 98/326 (30.06%), Postives = 147/326 (45.09%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGY-------YGNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDA---LTKSCKKCDCDSLGSTSENCDKLTGK--CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN----YPNCKKCLCSPHALSAD-CDLNNGKCKCGSNVINRICDKCENLHYWN-NRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLD--VTGCKKCPPCYNGQV---CNNEGICVCPPNTIGNLCNKC 3457
+C SG C+ C+ G+ YG +C+ C C+ + CD V+G C C +N++G C+ C GFYG+A C+ C C LG + +G CD C + +CD C G Y + C PC C +S+ C+ +G+C C+ +G CE+C GYF NC+ C CS + ++ CD G+C+C NV R CD C +W+ + C PC C G+++ CD G C C + + C ++G+ TGC C G C+ G C C P +G C++C
Sbjct: 349 QCPPGYSGTSCEACVRGHRRVNGSLYGG-----ACEPCDCHGHATECDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCAC-PLGFSPTCRVGPSGAMLCDRCAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFGDAIVAKNCQPCKCSSNGSQSNTCDAVTGQCECKPNVAGRQCDHCVPNCWWDAQQRKCQPCRCSLEGAVSSACDKH-GRCACRPEFSGKRCEPSQARHYGMSQQPTGCAPCNCNSFGAFSFDCDESGQCHCQPGVVGRQCDRC 667 HSP 3 Score: 108.612 bits (270), Expect = 7.541e-24 Identity = 99/332 (29.82%), Postives = 138/332 (41.57%), Query Frame = 2
Query: 2858 CDCRTNFVNRQCDSCTSGLY-----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSADCDLNNGKC----KCGSNVINRICDKCENLHYWNNRT---DCVPCNCGRG---SLNPKCDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC---YNGQVCNN----EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVC 3751
C C + C++C G L C PC+C C+ SG C C N G RCE+C+ G++ +C+ C C P S C + +C CD+C + +Y + C PC C S CD TG C +C + C +C GYFG D K C PC NG N G C C PN G C+ C + W + C+ C C + G+ S+ C+K G CAC+ F G +C+ +YG C PC+C G S + CD+ +GQC C+ V+G +C C
Sbjct: 348 CQCPPGYSGTSCEACVRGHRRVNGSLYGGACEPCDCH-----GHATECDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCAC-PLGFSPTCRVGPSGAMLCDRCAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFG-DAIVAKNCQPCKCSSNGSQSNTCDAVTGQCECKPNVAGRQCDHCVPNCWWDAQQRKCQPCRCSLEGAVSSACDK-HGRCACRPEFSGKRCEPSQARHYGMSQQPTGCAPCNCNSFGAFSFD---CDE-SGQCHCQPGVVGRQCDRC 667 HSP 4 Score: 87.0409 bits (214), Expect = 4.181e-17 Identity = 76/296 (25.68%), Postives = 116/296 (39.19%), Query Frame = 2
Query: 4364 VKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTA--VEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCN-----------------------------------------------------QQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPN--YVVEKKTCQSC 5068
+ L+ + PL ++ + + + V A VE C C G C+ + G+ + +GS+ G + C+C+G++ CD V+G+C NC N G +C+ C GFY N + C+PC CP F+ C S L +C GY+G C+ C G+YG+P + G C+ C CN GSQS C+ G C+C P V G +CD C PN + +++ CQ C
Sbjct: 308 LGLHRAPLPAPLSPHRLSSVTLEVAAEGTRGAVAASDVELCQCPPGYSGTSCEACVRGHRR---------VNGSLYGGA-----CEPCDCHGHATECDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCACP---LGFSPTCRVGPSGAMLCDRCAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFGDAIVAKNCQPCKCSSNGSQSNTCDAVTGQCECKPNVAGRQCDHCVPNCWWDAQQRKCQPC 586 HSP 5 Score: 81.6481 bits (200), Expect = 2.161e-15 Identity = 68/213 (31.92%), Postives = 89/213 (41.78%), Query Frame = 2
Query: 3260 CDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEK 3898
C + G C +VARR+ S G F G VC N C NT G C C ++ + + C+ CNC GS S +C S C CK + G +C+RC GY GFP C C C+ G+ + D C CK NV G C C+ G + + GC CFCFGV+ C +I R H D +
Sbjct: 3 CHGKAGECYYDPDVARRNLSLNTAGKF-------------VGGGVCLN-----CSQNTAGVNCEICVDEYFRPPKNP-CRPCNCNPVGSLSKVCINNSRSCRCKPGYGGERCNRCAFGYKGFPKCVKCKCSTEGSINK-----DPCKEPCICKSNVQGKHCDSCKPGHYNLQESNALGCTECFCFGVTRMCDSIPWARAKVRGQAARHGADGR 191 HSP 6 Score: 77.7962 bits (190), Expect = 2.553e-14 Identity = 60/184 (32.61%), Postives = 85/184 (46.20%), Query Frame = 2
Query: 4484 CNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNC--NG-YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQC 5026
C T G HC+ +GY+ + + N + C C NG S CD+VTG C+ C N G +C C + D +C+PC+C S + C + +C CR +SGK CE Q YG + C C+CN G+ S +C+E G C C PGV+G +CD+C
Sbjct: 508 CVGQTSGWHCERCTTGYFGDAI----------------VAKNCQPCKCSSNGSQSNTCDAVTGQCE-CKPNVAGRQCDHCVPNCWW-DAQQRKCQPCRC-SLEGAVSSACDK-----HGRCACRPEFSGKRCEPSQARHYGMSQQPTGCAPCNCNSFGAFSFDCDESGQCHCQPGVVGRQCDRC 667 HSP 7 Score: 60.8474 bits (146), Expect = 4.145e-9 Identity = 75/289 (25.95%), Postives = 109/289 (37.72%), Query Frame = 2
Query: 824 IRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPV------AVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCV--SNGEDIGDQKPGDCICKKGYEGVNCDLCSIGY---ERYPNGECLPCPC--SIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
+ H +L L H+ L + S+ +E+ A + + V C C G++CE C R + V CE C+C+ + EC + G C+NC+ N+ G CE+C+ GFY N + C PC C LG +C +G + D+ C GY G CD C+ GY P G C PC C ++D S + C V S C+ C GYF D+ + C+PC C
Sbjct: 294 LPVPLCPHPSLHTPLGLHRAPLPAPLSPHRLSSV-TLEV---------AAEGTRGAVAASDVELCQCPPGYSGTSCEAC-----VRGHRRVNGSLYGGACEPCDCHGHATECD------------------DVSGNCLNCEHNSVGPRCERCLPGFY--GNAQEGTPHDCQPCACP-LGFSPTCRVGPSGAMLCDR------CAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFG-DAIVAKNCQPCKC 539 HSP 8 Score: 60.077 bits (144), Expect = 9.063e-9 Identity = 70/242 (28.93%), Postives = 96/242 (39.67%), Query Frame = 2
Query: 950 CICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLF--NQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHG---LGTIGSCVSNGEDIGDQKPGDCI-CKKGYEGVNCDLCSIGY--ERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
C C+ HA +CD V N + NC+HN+ G CE C P F N + P C+ C C L S G +C C G C++C G+Y V C PCQC+G L T G+C D G+C+ C G +C+ C+ GY + C PC CS +GS + C CK +V N +++ A C+PC C
Sbjct: 381 CDCHGHATECDDVSGNCL-----NCEHNSVGPRCERCLPGFYGNAQEGTP-HDCQPCAC--------------PLGFSPTCRVGPSGAMLCDRCAPGYAGARCDRCADGYYGSPGVPGGS---CQPCQCNGNVDLSTPGAC--------DAVTGECLRCVGQTSGWHCERCTTGYFGDAIVAKNCQPCKCSSNGSQSNTCDAVTGQCECKPNVAGRQCDHCVPNCWWD---AQQRKCQPCRC 588 HSP 9 Score: 56.9954 bits (136), Expect = 6.512e-8 Identity = 41/114 (35.96%), Postives = 51/114 (44.74%), Query Frame = 2
Query: 2861 DCRTNFVNRQCDSCTSGLYLKDK-KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY 3199
+C N C+ C + K C PCNC G +S K C S C C G+RC +C GY +P C KC CS S + D C C SNV + CD C+ HY
Sbjct: 37 NCSQNTAGVNCEICVDEYFRPPKNPCRPCNCNPVGSLS--KVCINNSRSCRCKPGYGGERCNRCAFGYKGFPKCVKCKCSTEG-SINKDPCKEPCICKSNVQGKHCDSCKPGHY 147 HSP 10 Score: 54.299 bits (129), Expect = 5.355e-7 Identity = 55/230 (23.91%), Postives = 96/230 (41.74%), Query Frame = 2
Query: 4661 GDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLN-CNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKV 5347
G C NC N G C++C + +++ KN C PC C N + +++ + C+C+ GY G+ C C G+ G P C C C+ +GS + + C E C C V G CD C P + + +S GCT+ + + +++ W + + I +++ + +L +++ + M A+ YG K TR +K
Sbjct: 33 GVCLNCSQNTAGVNCEICVDEYFRPPKN--PCRPCNC-----NPVGSLSKVCINNSRSCRCKPGYGGERCNRCAFGYKGFPK----CVKCKCSTEGSINKDPCKE--PCICKSNVQGKHCDSCKPGHYNLQ---ESNALGCTECFCFGVTRMCDSI---PWARAKVRGQAARHGADGRINKKIHVMDITLWWTRLSLAVQD--TVSRGMMPTAQPDYGRGNKKMTRIHKT 241 HSP 11 Score: 53.1434 bits (126), Expect = 1.093e-6 Identity = 40/146 (27.40%), Postives = 63/146 (43.15%), Query Frame = 2
Query: 3410 ICVCPPNTIGNLCNKCSEDSWDFNETI---GCKKCNCYINGSYSNICNKISGICA-CKTAFFGSKCDRCNEGYYGFP------NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFG 3817
+C CPP G C C N ++ C+ C+C+ ++ C+ +SG C C+ G +C+RC G+YG +CQPC+C + + + + +C G +C C G + S G G C C C G
Sbjct: 347 LCQCPPGYSGTSCEACVRGHRRVNGSLYGGACEPCDCH---GHATECDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCACPLGFSPTCRVGPSGAMLCD-RCAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNG 488
BLAST of Laminin subunit alpha-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000128.1 (pep scaffold:Pmarinus_7.0:GL482156:314:14887:1 gene:ENSPMAG00000000114.1 transcript:ENSPMAT00000000128.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 239.965 bits (611), Expect = 3.351e-67 Identity = 193/650 (29.69%), Postives = 281/650 (43.23%), Query Frame = 2
Query: 1406 CKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGE------KIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKI----ELQSTLKGSLIDSPDVIIKCGDFRLF-----HSVSIEDRRLQRQVFI------YIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASG-----YYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS-CKRCKCNNNQE-----------SCDPVTGECR-CSYNTMGTECQLCKH---------GFYGDALTKS-----CKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKD--KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY-PN-CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKC 3184
CK G C+ C G+ YP EC PC C G C CK +V C C+ G F+L + NP+GC C+CFG+T +C+S I+++ GW+L +G ++ + T ++D YW AP YLG+ ++SYG + V+ + T G PD+I++ + H I R +RQ + ++ E + A++EE L + LL++ + + L ++ + +++ +E+C CP + G SC+ C G+ R + + G+C C C HS+ C+ Q+C HNT G HCE C G GD S + C CPCP + C E C C G +C+RC GY+GNP S CK C+CN N SCDPVTG C C ++ G CQ C+ G A +S C C CD G + CD +GKC C+ + C +C G Y D K C PC C + S C+ SG+C C +V G RCE C GY+NY PN C++C C+ + CD ++G+C C V CD+C
Sbjct: 1 CKPNVGGQRCEHCLPGFFGYP--ECRPCDCDAAGVSGAGCDAVTGQCLCKENVEGR-RCDVCRLGTFDLGAENPKGCTACFCFGVTGECTSSELHQDTIANMSGWRLLMGSGGGGEESVQVKHDETAQRAEATLSKRHVDAELE----LYWAAPHAYLGDQLSSYGGELSFRVRASGGQDGHRTPPGDH-QQPDLILRVVKIEIVTKNGSHMSLIH--RAERQPVVGETFHGSVRLLEGGFQHDASGRPASREELAMVLQDLQALLLRGRTGPGPSRLTLSHVVMETASANVSGPLARGVERCLCPASYRGGSCQECARGHYRDSRGSFLGRCLPCDCNGHSDACE------QHCDHNTRGDHCELCREGCLPRDVQGDEPS---SQCFPCPCPMT--IRSNQCCVQKPEGLEC-LCREGYEGKNCERCAPGYFGNPMVIGSECKACQCNGNAADDGGGPGVPGSSCDPVTGRCHECRHHRAGPRCQDCERVTVAWVRRPGVTLAATVRSLVACPCAACRCDKCG--MKGCDGRSGKCICKLGVMGHSCSTCEPGYYGFDSCKGCEPCECAVAALGSQ---CDPRSGRCRCPPSVTGRRCELCQPGYWNYGPNGCQRCECT----TGSCDPHSGRCSCKGGVSGEQCDQC 619 HSP 2 Score: 152.525 bits (384), Expect = 5.135e-38 Identity = 124/474 (26.16%), Postives = 197/474 (41.56%), Query Frame = 2
Query: 3566 CKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIF-----DEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIP----EQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQA--IGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYD----QCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCN 4939
CK G +C+ C G++G+P C+PC C G CD +TGQC CK+NV G +C VCR GTF GC +CFCFGV+ +C + H+ ++ + + + S++ D + A +R + +Y + LS + + V + G P +Q ++ + + + S+ H + V+ + + + + +R+ M L ++ +L L + P ++ +++ VMET A G VE+C+C A G CQ G+Y++ + L C+CNG+S C+ Q+C N G+ C+LCREG D D QC PC CP N ++ L+C CR GY GK+CE C G++GNP + G CK+C CN
Sbjct: 1 CKPNVGGQRCEHCLPGFFGYPECRPCDCDAAGVSG---AGCDAVTGQCLCKENVEGRRCDVCRLGTFDLGAENPKGCTACFCFGVTGECTSSELHQDTIANMSGWRLLMGSGGGGEESVQVKHDETAQRAEATLSKRH---VDAELELYWAAPHAYLGDQLSSYGGELSFRVRAS--GGQDGHRTPPGDHQQPDLILRVVKIEIVTKNGSHMSLI-----------HRAERQPVVGETFHGSVRLLEGGFQHDASGRPASREELAMVLQDLQAL---LLRGRTGPGPSRL-TLSHVVMETASANVSGPLARGVERCLCPASYRGGSCQECARGHYRD--------------SRGSFLGRCLPCDCNGHSDACE------QHCDHNTRGDHCELCREGCLPRDVQGDEPSSQCFPCPCP---MTIRSNQCCVQKPEGLECLCREGYEGKNCERCAPGYFGNPMVIGSECKACQCN 428 HSP 3 Score: 104.76 bits (260), Expect = 9.521e-23 Identity = 99/347 (28.53%), Postives = 133/347 (38.33%), Query Frame = 2
Query: 2708 ECRCSYNTMGTECQLCKHGFYGDA---LTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFC--NTTSGQCFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPHALSAD---------CDLNNGKC-KCGSNVINRICDKCENLHY-WNNRTD--------------CVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC------YNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRC 3604
C C + G CQ C G Y D+ C CDC+ S+ C++ C N C+ C G +D +C PC C + I S + C +C C E G CE+C GYF P CK C C+ +A CD G+C +C + C CE + W R C C C + + CD +G C C V CS C+ GY+G D CK C PC Q G C CPP+ G C C W++ GC++C C + C+ SG C+CK G +CD+C
Sbjct: 292 RCLCPASYRGGSCQECARGHYRDSRGSFLGRCLPCDCNG---HSDACEQ-----HCDHNTRGDHCELCREGCLPRDVQGDEPSSQCFPCPCPMT--IRSNQCCVQKPEGLECLCREGYEGKNCERCAPGYFGNPMVIGSECKACQCNGNAADDGGGPGVPGSSCDPVTGRCHECRHHRAGPRCQDCERVTVAWVRRPGVTLAATVRSLVACPCAACRCDKCGMK-GCDGRSGKCICKLGVMGHSCSTCEPGYYGFDS--CKGCEPCECAVAALGSQCDPRSGRCRCPPSVTGRRCELCQPGYWNYGPN-GCQRCECT-----TGSCDPHSGRCSCKGGVSGEQCDQC 619 HSP 4 Score: 93.9745 bits (232), Expect = 2.553e-19 Identity = 88/332 (26.51%), Postives = 134/332 (40.36%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYYGNPKKKI--SCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHG-----FYGDALTKSCKKCDCDSLGSTSENC-DKLTG-KCDCRTNFVNRQCDSCTSGLY----LKDKKCIPCNC------EIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVE---GYFNYPN--------------CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKC 3457
C + G C C G+Y + + C C CN + ++C E C +NT G C+LC+ G GD + C C C +++ C K G +C CR + + C+ C G + + +C C C + G C+ +G+C C + G RC+ C + P C C C + CD +GKC C V+ C CE +Y +++ C PC C +L +CD +G C+C +V R C C GY+ GC++C C G + G C C G C++C
Sbjct: 295 CPASYRGGSCQECARGHYRDSRGSFLGRCLPCDCNGHSDAC-----EQHCDHNTRGDHCELCREGCLPRDVQGDEPSSQCFPCPCPMTIRSNQCCVQKPEGLECLCREGYEGKNCERCAPGYFGNPMVIGSECKACQCNGNAADDGGGPGVPGSSCDPVTGRCHECRHHRAGPRCQDCERVTVAWVRRPGVTLAATVRSLVACPCAACRCDKCGMKG-CDGRSGKCICKLGVMGHSCSTCEPGYYGFDSCKGCEPCECAVAALGSQCDPRSGRCRCPPSVTGRRCELCQPGYWNYGPNGCQRC-ECTTGSCDPHSGRCSCKGGVSGEQCDQC 619 HSP 5 Score: 62.003 bits (149), Expect = 1.883e-9 Identity = 84/347 (24.21%), Postives = 119/347 (34.29%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKDK-----KCIPCNCEIQGRISSEKFCNTTSGQC--FCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKC---DMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY-NGQVCNNE--------------GIC-VCPPNTIGNLCNKCSEDSWDFNETIG--------------CKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN---CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTF 3766
+C C ++ C C G Y + +C+PC+C N S C C N GD CE C EG C P + D + C PC C + +C E C C + ++C +C GYFG + +C C NG ++ G C C + G C C + + G C C C G C+ SG C CK G C C GYYGF + C+PC CA S +CD +G+C+C +V G +C++C+ G +
Sbjct: 292 RCLCPASYRGGSCQECARGHYRDSRGSFLGRCLPCDC------------NGHSDACEQHCDHNTRGDHCELCREG-----------CLPRDVQGD----------------------------EPSSQCFPCPCPMTIRSNQCCVQKPEGLECLCREGYEGKNCERCAPGYFGNPMVIGSECKACQCNGNAADDGGGPGVPGSSCDPVTGRCHECRHHRAGPRCQDCERVTVAWVRRPGVTLAATVRSLVACPCAACRCDKCGMKG--CDGRSGKCICKLGVMGHSCSTCEPGYYGFDSCKGCEPCECAVAALGS----QCDPRSGRCRCPPSVTGRRCELCQPGYW 581 HSP 6 Score: 57.3806 bits (137), Expect = 5.784e-8 Identity = 89/376 (23.67%), Postives = 141/376 (37.50%), Query Frame = 2
Query: 680 TYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYN----------------RYYYSLRNIEIGGKCI---CNNHADKCDKVIENGISVAKCNCKHNTCGSNCETC---CPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHG-LGTIGSCVSNGEDIGDQKPGDCI-CKKGYEGVNCDLC---SIGYERYPN-------GECLPCPCSIDGSVRTSFYRC---KPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC--FGITNQCSSLSAK 1690
T++ + L G +GR + E + ++ + L+ + L H+ + G L + + R +Y G+C+ CN H+D C++ +C HNT G +CE C C + + +P + C C C T+ + Q V K +E C+ C+E EG NCE+C G++ V+ +E C CQC+G G D G C C+ G C C ++ + R P + CPC+ + C C CK+ V C TC+ GY+ DS +GCEPC C + +QC S +
Sbjct: 219 TFHGSVRLLEGG--FQHDASGRPASREELAMVLQDLQALLLRGRTGPGPSRLTLSHVVMETASANVSGPLARGVERCLCPASYRGGSCQECARGHYRDSRGSFLGRCLPCDCNGHSDACEQ-----------HCDHNTRGDHCELCREGCLPRDVQGDEPSSQCFPCPC---------PMTIRSNQCCVQKPEGLE----CL-CREGYEGKNCERCAPGYFGNPMVIGSE---CKACQCNGNAADDGGGPGVPGSSCDPVTGRCHECRHHRAGPRCQDCERVTVAWVRRPGVTLAATVRSLVACPCAACRCDKCGMKGCDGRSGKCICKLGVMGHS-CSTCEPGYYGFDSC--KGCEPCECAVAALGSQCDPRSGR 561 HSP 7 Score: 56.225 bits (134), Expect = 1.105e-7 Identity = 64/241 (26.56%), Postives = 91/241 (37.76%), Query Frame = 2
Query: 1013 KCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKC----IKGFYRPWNVLATELY-----PCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGE-CLPCPCSIDG-----SVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAK 1690
+C C+ G NCE C P + P + C+ C CN N S + + G C C+ + G C+ C + RP LA + PC C+C G G D + G CICK G G +C C GY + + + C PC C++ R+ RC P + C+ C+ GY+N P GC+ C C T C S +
Sbjct: 392 ECLCREGYEGKNCERCAPGYFGNPMVIGSECKACQCN--GNAADDGGGPGVPGSSCDPVT-----GRCHECRHHRAGPRCQDCERVTVAWVRRPGVTLAATVRSLVACPCAACRCDKCGMKGC---------DGRSGKCICKLGVMGHSCSTCEPGYYGFDSCKGCEPCECAVAALGSQCDPRSGRCRCPP-------SVTGRRCELCQPGYWNY---GPNGCQRCEC--TTGSCDPHSGR 604 HSP 8 Score: 56.225 bits (134), Expect = 1.213e-7 Identity = 28/60 (46.67%), Postives = 33/60 (55.00%), Query Frame = 2
Query: 3011 CLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALS-ADCDLNNGKCKCGSNVINRICDKCE 3187
C NV G RCE C+ G+F YP C+ C C +S A CD G+C C NV R CD C
Sbjct: 1 CKPNVGGQRCEHCLPGFFGYPECRPCDCDAAGVSGAGCDAVTGQCLCKENVEGRRCDVCR 60 HSP 9 Score: 55.8398 bits (133), Expect = 1.764e-7 Identity = 58/211 (27.49%), Postives = 80/211 (37.91%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILIN--LKKCNCNGYSKV-----------CDSVTGDCQNCMGNREGNKCQLC---------REGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+C+C G +C+ GY+ GN ++I K C CNG + CD VTG C C +R G +CQ C R G CP A + + KC C++G G SC C+ G+YG + KG C+ C+C S G C+C P V G +C+ C P Y
Sbjct: 392 ECLCREGYEGKNCERCAPGYF-----------------GNPMVIGSECKACQCNGNAADDGGGPGVPGSSCDPVTGRCHECRHHRAGPRCQDCERVTVAWVRRPGVTLAATVRSLVA---CPCAACRCDKCGMKGCDGRSGKCICKLGVMGHSCSTCEPGYYGFDSCKG--CEPCECAVAALGSQCDPRSGRCRCPPSVTGRRCELCQPGY 580
BLAST of Laminin subunit alpha-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009682.1 (pep scaffold:Pmarinus_7.0:GL479835:738:11671:-1 gene:ENSPMAG00000008750.1 transcript:ENSPMAT00000009682.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 144.821 bits (364), Expect = 1.502e-37 Identity = 103/363 (28.37%), Postives = 167/363 (46.01%), Query Frame = 2
Query: 455 QVYQIVYILLKMGDSPRPGNWILER--SLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEM--FCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSV 1513
+ + I Y+ LK S RP ++ + + S+DG PW + SC + ++ N DDE CT +S L G + S + GR S N + + L +++++ IR + T ++ + + YYY++ + +GG+C CN HA +C ++N + C C+HNT G++CE C PL+N RPW C C+CN +++EC ++ ++ R G GG C NC++NT+G CE+C++ FYR A+E C PC C+ +G+ + G C CK+G G CD C G+ C PC C+ G++
Sbjct: 2 KAFDITYVRLKFHTS-RPESFAIYKRASMDG----PWTPYQYYSGSCEPTYGKR--------NRGFLRSGDDEQTPLCTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPV------LQDWVTSTEIRVTLNRLNTFGDEVFN-----DHKVLRSYYYAISDFAVGGRCKCNGHASEC---VKNELGALACKCRHNTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDP----------ELYRATGHGGHCTNCRDNTDGKTCERCLENFYR---RSASEH--CLPCNCNPVGSYSRQC--------DEQGRCSCKQGVMGERCDQCLPGFHSMDEAGCRPCTCNPAGTL 314 HSP 2 Score: 83.5741 bits (205), Expect = 8.217e-17 Identity = 56/175 (32.00%), Postives = 82/175 (46.86%), Query Frame = 2
Query: 2573 KCLHNTSGVHCDRCIEGYYGNPKKKIS------CKRCKCNNNQESC--DPVT-------GEC-RCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKK-CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEK 3046
KC HNT G C++C+ Y P + + C C CN + C DP G C C NT G C+ C FY + ++ C C+C+ +GS S CD+ G+C C+ + +CD C G + D+ C PC C G + C +G+C C +NV G C++
Sbjct: 167 KCRHNTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRATGHGGHCTNCRDNTDGKTCERCLENFYRRSASEHCLPCNCNPVGSYSRQCDE-QGRCSCKQGVMGERCDQCLPGFHSMDEAGCRPCTCNPAGTLED---CQPDTGRCVCKDNVEGYNCDR 337 HSP 3 Score: 75.485 bits (184), Expect = 3.862e-14 Identity = 63/198 (31.82%), Postives = 92/198 (46.46%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-----CRCSYNTMGTECQLCKHGFYGD-----ALTKSCKKCD-CDSLGSTSE--------NCDKLTGKC-DCRTNFVNRQCDSCTSGLYLK--DKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGY--FNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDK 3181
RCKCN + C V E C+C +NTMG +C+ C Y D A ++S +C CD G E G C +CR N + C+ C Y + + C+PCNC G S + C+ G+C C + V G+RC++C+ G+ + C+ C C+P DC + G+C C NV CD+
Sbjct: 146 RCKCNGHASEC--VKNELGALACKCRHNTMGADCEKCLP-LYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRATGHGGHCTNCRDNTDGKTCERCLENFYRRSASEHCLPCNCNPVGSYSRQ--CD-EQGRCSCKQGVMGERCDQCLPGFHSMDEAGCRPCTCNPAGTLEDCQPDTGRCVCKDNVEGYNCDR 337 HSP 4 Score: 67.781 bits (164), Expect = 1.168e-11 Identity = 57/175 (32.57%), Postives = 76/175 (43.43%), Query Frame = 2
Query: 3281 CKCAQNVARRDCSKC-----DVGYFGLDVTGCKKCPPCY-NGQV------------CNNEGICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKC 3742
CKC N DC KC D + +C C NG+ + G C C NT G C +C E+ + + + C CNC GSYS C++ G C+CK G +CD+C G++ C+PC+C GT L C TG+C CK NV G C
Sbjct: 166 CKCRHNTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRATGHGGHCTNCRDNTDGKTCERCLENFYRRSASEHCLPCNCNPVGSYSRQCDE-QGRCSCKQGVMGERCDQCLPGFHSMDEAGCRPCTCNPAGT----LEDCQPDTGRCVCKDNVEGYNC 335 HSP 5 Score: 58.5362 bits (140), Expect = 1.018e-8 Identity = 64/266 (24.06%), Postives = 101/266 (37.97%), Query Frame = 2
Query: 4295 NVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVT-------------AVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC-PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQ 5023
N NS + +D + VT ++ + D+V ++ Y + + A+G R C C T G C+ + Y ++ S + N + C+CNG + C G C NC N +G C+ C E FY+ + + C PC C P Y ++ C + +C C+ G G+ C+ C GF+ G C+ C CN G+ + G C C V G CD+
Sbjct: 94 NFDNSPVLQDWVTSTEIRVTLNRLNTFGDEVFNDHKVLRSYYYAISDF--AVGGRCKCNGHASECVKNELGALACKCRHNTMGADCEKCLPLYNDRPWHRATS------ESANECTL----CDCNGRADECYFDPELYRATGHGGHCTNCRDNTDGKTCERCLENFYRRSAS-EHCLPCNCNPVGSY--SRQCDE-----QGRCSCKQGVMGERCDQCLPGFHSMDEAG--CRPCTCNPAGTLEDCQPDTGRCVCKDNVEGYNCDR 337 HSP 6 Score: 56.9954 bits (136), Expect = 3.311e-8 Identity = 47/162 (29.01%), Postives = 69/162 (42.59%), Query Frame = 2
Query: 4616 KCNCNGYSKVC--DSVTGDCQNCMGNREGNKCQLC------REGFYKNDKNYDQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY-VVEKKTCQSC 5068
+C CNG++ C + + C N G C+ C R ++ ++C C C + F R CR GK+CE C + FY C C+CN GS S C+E G C C GV+G +CDQC P + +++ C+ C
Sbjct: 146 RCKCNGHASECVKNELGALACKCRHNTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRATGHGGHCTNCRDNTDGKTCERCLENFYRRSASEH-CLPCNCNPVGSYSRQCDEQGRCSCKQGVMGERCDQCLPGFHSMDEAGCRPC 306
BLAST of Laminin subunit alpha-2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006618.1 (pep scaffold:Pmarinus_7.0:GL481588:182:19210:1 gene:ENSPMAG00000005955.1 transcript:ENSPMAT00000006618.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 110.153 bits (274), Expect = 4.211e-24 Identity = 83/246 (33.74%), Postives = 112/246 (45.53%), Query Frame = 2
Query: 2441 CASGYYGDAKSKNLNSCKKCPCPR---LDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-KKISCKRCKCNNN-----QESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT-KSCKKCDCDSLGSTSENC---------DKLTGKCDCRTNFVNRQCDSCTS---GLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHAL 3109
CASGYYG + C+ C CP CS V CL +G CD+C G+YG+ + C+ C+CN N SCD TG C RC +T G+ CQ C+ G+YGDA + + C+KC C + G+ +C D +G+C C N CD CT GL + C PC C+ S+ C+ +GQC CL G C +C E ++ P K C H L
Sbjct: 4 CASGYYGSPGAGE--QCRPCQCPEGPGTGRHFASGCSHDPRSRAVACACLPGFTGARCDQCSPGHYGDLRVAGGRCQPCQCNGNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYGDATSQRRCQKCTCVTAGTERSSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGLS-QGTGCRPCGCDPA--HSAGPSCSEFNGQCHCLPGFGGLTCSECQENHWGEPRVKCSECLTHNL 244 HSP 2 Score: 90.5077 bits (223), Expect = 5.413e-18 Identity = 66/246 (26.83%), Postives = 98/246 (39.84%), Query Frame = 2
Query: 2405 CTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPC-PRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKC------------NNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK-KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVN---GDRCEKCVEGYFN-YPNCKKC 3088
C GA C+ C+ G+YGD + C+ C C +D C + C +CL +T G C C GYYG+ + C++C C + +CDP +G+CRC N G C C +G + C+ C CD S +C + G+C C F C C + + + KC C + C C V G RC++C G+ +P C+ C
Sbjct: 50 CLPGFTGARCDQCSPGHYGDLRVAG-GRCQPCQCNGNIDPTDAASCDGRTGR---CLRCLRDTEGSACQNCRPGYYGDATSQRRCQKCTCVTAGTERSSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGLSQGTGCRPCGCDPAHSAGPSCSEFNGQCHCLPGFGGLTCSECQENHWGEPRVKCSEC--------LTHNLCMAQLAYCLFRPPVRSETGRRCDRCARGFSGAFPACEPC 283 HSP 3 Score: 84.3445 bits (207), Expect = 3.597e-16 Identity = 73/252 (28.97%), Postives = 104/252 (41.27%), Query Frame = 2
Query: 2249 CNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNH-----SNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCH--------KCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQE---SCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCR---TNFVNRQCDSCTSGLYLKDKKCIPCN 2947
C C PGF G C+ C G+ V G+C+ C C + + +CD G C C +T G+ C++C GYYGDA S+ C+KC C + SS + C +CL N G+ CDRC ++G + C+ C C+ SC G+C C G C C+ +G+ K C +C +L C C R + R+CD C G C PC+
Sbjct: 48 CACLPGFTGARCDQCSPGHYGDLRVAG-GRCQPCQCNGNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYGDATSQ--RRCQKCTCVTAGTER----SSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWG-LSQGTGCRPCGCDPAHSAGPSCSEFNGQCHCLPGFGGLTCSECQENHWGEPRVK-CSECLTHNL------CMAQLAYCLFRPPVRSETGRRCDRCARGFSGAFPACEPCH 284 HSP 4 Score: 84.3445 bits (207), Expect = 3.659e-16 Identity = 66/236 (27.97%), Postives = 95/236 (40.25%), Query Frame = 2
Query: 3038 CEKCVEGYFNYPN----CKKCLCSPHALSADCDLNNG----------KCKCGSNVINRICDKCENLHYWNNRT---DCVPCNCGRGSLNP----KCDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC--------------YNGQ-VCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN 3631
C +C GY+ P C+ C C P +G C C CD+C HY + R C PC C G+++P CD TG C +C ++ C C GY+G D T ++C C +G C+ G C C PN G C++C+ + W ++ GC+ C C S C++ +G C C F G C C E ++G P
Sbjct: 1 CCRCASGYYGSPGAGEQCRPCQC-PEGPGTGRHFASGCSHDPRSRAVACACLPGFTGARCDQCSPGHYGDLRVAGGRCQPCQCN-GNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYG-DATSQRRCQKCTCVTAGTERSSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGLSQGTGCRPCGCDPAHSAGPSCSEFNGQCHCLPGFGGLTCSECQENHWGEPR 233 HSP 5 Score: 70.8626 bits (172), Expect = 5.104e-12 Identity = 49/166 (29.52%), Postives = 79/166 (47.59%), Query Frame = 2
Query: 4613 KKCNCNGY-----SKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESS-----LELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKT-CQSCYS 5074
+ C CNG + CD TG C C+ + EG+ CQ CR G+Y + + +C+ C C +A + +C + + +C+C G +C+ C +G +G C+ C C+ S +C+EF G C C PG G+ C +C N+ E + C C +
Sbjct: 78 QPCQCNGNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYGDATSQRRCQKCTCVTAGTERS-SCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGLS-QGTGCRPCGCDPAHSAGPSCSEFNGQCHCLPGFGGLTCSECQENHWGEPRVKCSECLT 241 HSP 6 Score: 60.8474 bits (146), Expect = 5.247e-9 Identity = 40/130 (30.77%), Postives = 59/130 (45.38%), Query Frame = 2
Query: 4712 CREGFYKNDKNYDQCEPCQCPSA---HYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGN-PIKGIPCKSCDCNQ--QGSQSLNCN-EFGIC-QCSPGVLGIKCDQCAPNY---VVEKKTCQSC 5068
C G+Y + +QC PCQCP +FA C+ S + C C G++G C+ C G YG+ + G C+ C CN + + +C+ G C +C G C C P Y ++ CQ C
Sbjct: 4 CASGYYGSPGAGEQCRPCQCPEGPGTGRHFASGCSHDPRSRAVACACLPGFTGARCDQCSPGHYGDLRVAGGRCQPCQCNGNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYGDATSQRRCQKC 133
BLAST of Laminin subunit alpha-2 vs. Ensembl Nematostella
Match: EDO36523 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF3]) HSP 1 Score: 395.586 bits (1015), Expect = 4.545e-112 Identity = 306/1062 (28.81%), Postives = 495/1062 (46.61%), Query Frame = 2
Query: 203 KITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHP---IEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNW-ILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKV-IENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRC--KPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSL---SAKNLK---ISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKC-GDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVH--LALDEMPHIEKCNCPPGFNGLSCESCESGY-RRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK----ISCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMG---TECQLCKHGFYGDAL---TKSCKKCDCDSLGSTSENCDKLT--------GKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKC-VEGY-FNYPNCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKC-ENLHYWNNRTDCVPC 3229
++ S+ TCG P+ FC + +++ C++C++ + HP I K D + WWQS ++ + + V +T+DL + Y + YI ++ SPRP + I +++ ++P+ +F+ T Y + TTN CT +S L G + S + R N + P L +++A +R + T ++ + + ++ YYY++ ++ IGG+C CN HA +C++ + +G + +C C+H+T G +CE C PL+N RPW + C CNCN + C +++ + R G GG C NC++NT+G NCE+C + FYR T L PC C C+ +G++ S N E G C CK G G+ CD C Y + C PC C G+ + +C K C CK HV C C++GYF L+++NP GC+ C+C+G + C + +++N+ + L+GW + G+ F S + E+ + A Y+GN +SYG F ++ ++ T+ G +D+ D+ I+ G ++ ++ + F + + + T +F L + + I+ Y + +L+ V+ + +++ +E+C+CP + G +CE C+ GY R+ + QC C CY HS+TCD + G+C NC HNT G C+ C GYYG+ + CKKCPC + C + N VC C G CD C +GYYG+P K +C++C C+ N + SCD +TG+C +C +NT ++C+LC G+YG+A CK C+C G+ S T G+C C N +CD+C + K C PCNC+ G S C+ T+GQC C V G RC++C ++ Y F+ C C C+ +L+ CD G C+C V+ CD C EN H N C+ C
Sbjct: 43 RVISNNTCGDP-PKKFCVQTGVLEVRKE--------CEMCNSSDPALEHPALKITDIKDDTNRTWWQSETLLENPD-KPVTLTLDLGKSYDVSYIRIRF-RSPRPESMAIYKKTTPSSKWIPYQYFSATCEKTYGV----------TTNGIVDPRNQQVALCTDAFSTITPLTGGNVAFSTLEDRPDAYN-----FDNSP-ALQEWVTAVAVRIHLRRMNTFGDEV-----FGDPNVLRSYYYAIIDLSIGGRCKCNGHASECEETRLWDGQTRLQCKCQHDTEGVDCERCKPLYNDRPWARASSRNANECVRCNCNGLAESCVFDEALY----------RQTGHGGRCQNCKQNTDGPNCERCKENFYR-----KTSLEPCVACGCNPVGSV-SLQCNSE-------GQCRCKPGVTGIKCDKCQANYFGFSQTGCRPCQCFGPGT-DPAKTQCNEKGECTCKRHVVGAK-CTLCRSGYFGLEASNPHGCKQCFCYGHSTVCKAAPGYTSRNITTDFTTGLEGWAVENDQGDSAG---FVSVTAIGKES------------LFARASLVYIGNRRSSYGQTFGFMFRV---GTVDGMQVDNGDITIEGEGGLKITTQLTAQGNPKPGVAFQQYRYRLHEDASFGWRPSLTSHDFQRLLTSIKSIKIRMNYAD--QGTGMLDGMFFESAVYDPTSPNQVNWVEQCSCPKEYKGNNCEQCKDGYTRQAGSTDRYAQCVPCQCYGHSDTCDPVTGKC-NCKHNTTGIRCDKCIDGYYGNPTQGTPDDCKKCPC-----KFGTRCIQIGA-NVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNCSGNIDPNAIGSCDRLTGDCLKCIHNTRNGPISQCELCAIGYYGNATHYPKPQCKACECYVQGTVSPPGHDPTLPFPCDDKGRCTCLPNVSGDKCDTCRPEYWNVASGKGCEPCNCDTTG--SWNNTCSVTTGQCHCKPGVTGRRCDQCSIDHYAFSELGCLACNCNLQGSLNVTCD-EQGVCQCRPGVLGIKCDSCQENFH--NLTAGCIAC 1015 HSP 2 Score: 141.354 bits (355), Expect = 3.972e-33 Identity = 117/412 (28.40%), Postives = 182/412 (44.17%), Query Frame = 2
Query: 2825 TSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNR------TDCVPCNCGRGSLNP----KCDMETGNC-KCAQNVARRDCSKCD---VGYFG----LDVTGCKKC---------PPCYNGQV---CNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC-FCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDY 3925
TS N +C C + C+ C G + +C+PC C C+ +G+C C N G RC+KC++GY+ P +CKKC C N C + +CD C++ +Y + T C CNC G+++P CD TG+C KC N S+C+ +GY+G CK C PP ++ + C+++G C C PN G+ C+ C + W+ GC+ CNC GS++N C+ +G C CK G +CD+C+ +Y F C C+C G+ + CD+ G CQC+ V+G KC C++ GC +C C+ V +K L ++ + IY F+ +S+ D+
Sbjct: 659 TSPNQVNWVEQCSCPKEYKGNNCEQCKDGYTRQAGSTDRYAQCVPCQC-----YGHSDTCDPVTGKCNCKHNTTGIRCDKCIDGYYGNPTQGTPDDCKKCPCKFGTRCIQIGANVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNCS-GNIDPNAIGSCDRLTGDCLKCIHNTRNGPISQCELCAIGYYGNATHYPKPQCKACECYVQGTVSPPGHDPTLPFPCDDKGRCTCLPNVSGDKCDTCRPEYWNVASGKGCEPCNCDTTGSWNNTCSVTTGQCHCKPGVTGRRCDQCSIDHYAFSELGCLACNCNLQGSLN---VTCDE-QGVCQCRPGVLGIKCDSCQENFH----NLTAGCIACPPCYDVIQKIVGGLRRKLYD-LIYIIDRFNTSSSVVVDVDF 1055 HSP 3 Score: 140.198 bits (352), Expect = 8.920e-33 Identity = 141/536 (26.31%), Postives = 223/536 (41.60%), Query Frame = 2
Query: 3419 CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRF-GWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGK----WVISQYQNDILDKFIILEDSNV-INSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPI--KGIP--CKSCDCNQQGSQSLNCNEFGIC 4984
C NT G C +C E+ + C C C GS S CN G C CK G KCD+C Y+GF C+PC C GT + T+C++ G+C CK++V+G KC +CR G F + + GC CFC+G S C A Y++ ++ ++ GW + + + + SLF R+ + + N+ R QT FM + GM+ + + + G QY ++ + ED++ +LT F L ++ S+K+++ ++ V + T +V VE+C C GN+C+ GY ++ +GS + + C C G+S CD VTG C NC N G +C C +G+Y N D C+ C C F C +I +++ + C G+ G C+ CQ G+YG+P+ +G P C+ C+C S +++ N G C
Sbjct: 352 CKQNTDGPNCERCKENFYRKTSLEPCVACGCNPVGSVSLQCNS-EGQCRCKPGVTGIKCDKCQANYFGFSQTGCRPCQCFGPGTDPAK-TQCNE-KGECTCKRHVVGAKCTLCRSGYFGLEASNPHGCKQCFCYGHSTVCKA-------------------------APGYTSRNITTDFTTGLEGWAVENDQGDSAGFVSVTAIGKESLFARASLVYIG-----NR--RSSYGQTFGFMFRVGTVDGMQVDNGDITIEGEGGLKITTQLTAQGNPKPGVAFQQY------RYRLHEDASFGWRPSLTSHDFQRLLTSIKSIKIRMNYADQGTGMLDGMFFESAVYDPTSP--NQVNWVEQCSCPKEYKGNNCEQCKDGYTRQ---------AGSTDRYAQCV----PCQCYGHSDTCDPVTGKC-NCKHNTTGIRCDKCIDGYYGNPTQGTPDDCKKCPC-----KFGTRCIQIGANV-VCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNC----SGNIDPNAIGSC 820 HSP 4 Score: 131.724 bits (330), Expect = 3.690e-30 Identity = 112/349 (32.09%), Postives = 150/349 (42.98%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGY---YGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY-------LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENV-NG--DRCEKCVEGYFN------YPNCKKCLC------SPHA----LSADCDLNNGKCKCGSNVINRICDKCENLHYWN--NRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQV---CNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC 3505
C G +C++C +GY G+ + C C+C + ++CDPVTG+C C +NT G C C G+YG+ + CKKC C G+ DC V CD C G Y C CNC ++ C+ +G C C+ N NG +CE C GY+ P CK C C SP L CD + G+C C NV CD C YWN + C PCNC GS N C + TG C C V R C +C + ++ GC C G + C+ +G+C C P +G C+ C E+ N T GC C
Sbjct: 672 CPKEYKGNNCEQCKDGYTRQAGSTDRYAQCVPCQCYGHSDTCDPVTGKCNCKHNTTGIRCDKCIDGYYGNPTQGTPDDCKKCPC-KFGTRCIQIGANVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNCSGNIDPNAIGSCDRLTGDCLKCIHNTRNGPISQCELCAIGYYGNATHYPKPQCKACECYVQGTVSPPGHDPTLPFPCD-DKGRCTCLPNVSGDKCDTCRP-EYWNVASGKGCEPCNCDTTGSWNNTCSVTTGQCHCKPGVTGRRCDQCSIDHYAFSELGCLACNCNLQGSLNVTCDEQGVCQCRPGVLGIKCDSCQENF--HNLTAGCIAC 1015 HSP 5 Score: 82.8037 bits (203), Expect = 2.228e-15 Identity = 60/200 (30.00%), Postives = 95/200 (47.50%), Query Frame = 2
Query: 4676 CMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY---VVEKKTCQSCYSGCTK----------DLLVRIDTLNET---VINFKWKTLPSTLDLRLNKINISIQMSSELLNSIN 5227
C+ N G+KC CR ++ N + CEPC C + ++ C S +C C+ G +G+ C+ C Y + C +C+CN QGS ++ C+E G+CQC PGVLGIKCD C N+ C CY K DL+ ID N + V++ ++ + L ++ ++ + S + S+N
Sbjct: 893 CLPNVSGDKCDTCRPEYW-NVASGKGCEPCNCDTTG-SWNNTC----SVTTGQCHCKPGVTGRRCDQCSIDHYA--FSELGCLACNCNLQGSLNVTCDEQGVCQCRPGVLGIKCDSCQENFHNLTAGCIACPPCYDVIQKIVGGLRRKLYDLIYIIDRFNTSSSVVVDVDFEKRLALLKEQVERLLGQSRRSQQYRQSLN 1084 HSP 6 Score: 73.9442 bits (180), Expect = 1.106e-12 Identity = 64/204 (31.37%), Postives = 94/204 (46.08%), Query Frame = 2
Query: 4523 ISGYYKEYFNLPNSTKSGSMRTGNRILINLK---KCNCNGYSKVCDSVT-GDCQN-----CMGNREGNKCQLC------REGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLEL-----KCQ-CRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKT-CQSC 5068
++ + E F PN +R+ +I+L +C CNG++ C+ D Q C + EG C+ C R + +N ++C C C A++C E+ +CQ C+ G +CE C++ FY PC +C CN GS SL CN G C+C PGV GIKCD+C NY +T C+ C
Sbjct: 234 MNTFGDEVFGDPNV-----LRSYYYAIIDLSIGGRCKCNGHASECEETRLWDGQTRLQCKCQHDTEGVDCERCKPLYNDRPWARASSRNANECVRCNC----NGLAESCVFDEALYRQTGHGGRCQNCKQNTDGPNCERCKENFY-RKTSLEPCVACGCNPVGSVSLQCNSEGQCRCKPGVTGIKCDKCQANYFGFSQTGCRPC 427 HSP 7 Score: 70.4774 bits (171), Expect = 1.367e-11 Identity = 67/259 (25.87%), Postives = 112/259 (43.24%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTG-------ECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCE--IQGRISSEKFCNTTS--GQCF-CLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNP---KCDMETGNCKCAQNVARRDCSKCDVGYFGLDVT---GCKKC 3370
RCKCN + C+ +C+C ++T G +C+ CK + ++ +S N ++ C+ CNC + + E T G+C C +N +G CE+C E ++ + C C C+P ++S C+ + G+C+C V CDKC+ ++ ++T C PC C +P +C+ E G C C ++V C+ C GYFGL+ + GCK+C
Sbjct: 263 RCKCNGHASECEETRLWDGQTRLQCKCQHDTEGVDCERCKPLYNDRPWARA-----------SSRNANE-----------------------------CVRCNCNGLAESCVFDEALYRQTGHGGRCQNCKQNTDGPNCERCKENFYRKTSLEPCVACGCNPVGSVSLQCN-SEGQCRCKPGVTGIKCDKCQANYFGFSQTGCRPCQCFGPGTDPAKTQCN-EKGECTCKRHVVGAKCTLCRSGYFGLEASNPHGCKQC 479 HSP 8 Score: 68.1662 bits (165), Expect = 7.407e-11 Identity = 47/152 (30.92%), Postives = 69/152 (45.39%), Query Frame = 2
Query: 4616 KCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQ--SLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+CNCNG ++ C G CQNC N +G C+ C+E FY+ + + C C C ++ + E +C+C+ G +G C+ CQ ++G G C+ C C G+ CNE G C C V+G KC C Y
Sbjct: 323 RCNCNGLAESCVFDEALYRQTGHGGRCQNCKQNTDGPNCERCKENFYRK-TSLEPCVACGCNPV------GSVSLQCNSEGQCRCKPGVTGIKCDKCQANYFGFSQTG--CRPCQCFGPGTDPAKTQCNEKGECTCKRHVVGAKCTLCRSGY 465 HSP 9 Score: 61.2326 bits (147), Expect = 8.077e-9 Identity = 45/145 (31.03%), Postives = 67/145 (46.21%), Query Frame = 2
Query: 4673 NCMGNREGNKCQLCREGFYK---NDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIP--CKSCDCNQQGSQSLNCNEFGIC-QCSPGVLGIKCDQCAPNYVVE-------KKTCQSC 5068
+C +GN C+ C++G+ + + Y QC PCQC Y + C + KC C+ +G C+ C G+YGNP +G P CK C C + G++ + +C C G +G CD C Y + TCQ C
Sbjct: 671 SCPKEYKGNNCEQCKDGYTRQAGSTDRYAQCVPCQC----YGHSDTCDPVTG----KCNCKHNTTGIRCDKCIDGYYGNPTQGTPDDCKKCPC-KFGTRCIQIGANVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKC 806
BLAST of Laminin subunit alpha-2 vs. Ensembl Nematostella
Match: EDO39502 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9X8]) HSP 1 Score: 293.508 bits (750), Expect = 1.704e-79 Identity = 319/1205 (26.47%), Postives = 498/1205 (41.33%), Query Frame = 2
Query: 179 DGLLMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNR----NETKRHPIEFAKSDDD----NLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKC----DKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTI--GSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGY----ERYPNGECLPCPCSIDGSVRTSFYRCKPP---CNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC---FGITNQCSSLSAKNLKISDLKGWKLTTF-TGEKIALPKFTSFQYLTSENSYIDE----RFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLI--DSPDVIIKCGDFRLFHSVSIED---------RRLQ------RQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSC---------------ESCESGYRRVNNVLYQGQCEACAC--------YNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGST---SENCDKLTGK------CD-CRTNFVNRQCDSCTSGLY----LKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN---YPNCKKCLCSPHALSA----DCDLNNGKCKCGSNVINRICDKCENLHYWN--NRTDCVPCN-CGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQV---CNNE-GICVCPPNTIGNLCNKCSEDS 3469
D L+ KI +S+TCG GPE +C + N + C +CD+R N H ++ S D + WWQS + +NV + +DL+ + +++++ + RP ++ERS D G+ + + +F+ C +F P + +P + D++ CT YS + +GE+I + + I N ++ N L IR F K TL DL+ + I +YYY++ ++ I G C C HA++C + G+ +CNC HNT G NCE C + PW+P C +CNCN + +C ++ V +V+ GGVC +C+ NT G NCE+C Y+ + ++ C C C G + G C + + G CICK+ GV CD C G+ P+G C C C+ G++ C P C CK +V +D C C + Y+ L S+ P GC C C NQC + + + G + TG LP ++ + + E + WT G + GT Y + S L+ ++ D G +R +S D RR R V I + P+ + PD E+ +S ++V ++ + + SI +I L V ++D E F+ C + C+ +++V+Y G AC C N + CD + G+C+ C N G C+ CA G +G + C C C R+ + C+ + + +C + SG C C +G P C+ C+C + SC+P TG C C +NT+G C+ C GFYG A + CK+C C S SE+C T CD C+ + RQC+ C G Y + C PC+C + C+ +G C CL N G CE+C GYF NC C+C + C+ G+C C +V + CD+C +WN + C C+ C GSL C+ TG C+C C +C+ ++G GC C G V C+ + G C+C IG+ C+ C+ D+
Sbjct: 29 DLLVGRENKIYASSTCGLRGPEEYCVVS---------NLQDYGDCHICDSRPYQTNPQFSHVPKYMLSSFDGRKKDEWWQSEN-----GRENVYLQLDLESEFHFTHLIMRF-RTFRPAGMVIERSWDYGKKWQIYRYFSA---DCDKIF----PGI---PKGEPKDI--DDVICTNKYSTVEPSTNGEVIFRALKSQIPIKNAYSSKVQ-------NLLKITNIRINFTKLHTLGDDLLDPR----PEIKRKYYYAVYDLVIRGSCSCYGHAEQCLPEPGQPTTPGMVYGRCNCTHNTTGKNCERCQDGYFDVPWRPADADNPNECRKCNCNGHAVKCRFDPAVYQASGNVS-------GGVCQDCEHNTVGRNCERCKPLHYQDPSKKFSDPDACIACNCDPTGGVNGGECEAKNDPAAGLVAGRCICKRNVGGVRCDRCKAGFFNLTTDNPDG-CKACGCNPLGTI----GECNPDTGRCTCKRYVEGED-CDRCASEYWGL-SSTPAGCSNCACDVGGSYDNQCDKQTGQCRCRKGITGRQCERVKTGNYYPLPDHLIYESEEARAVGVSEVARQEYPKNGAATWTGD----GLLDVADGTAVVYSITNIPNSGFYTPLLRYETKDA----GQWRAVVQISRPDGKPLTTGACRRPSGRDEYYRNVTIALNPSRRYV--TLPDDVCL-EKGVSYDIRV--RFVRDPVSNTRDSIFTDSIVLL--PVPGSVDIFQGSEGAKRQAQFDLYQCLDYHLAASQPRPKLSDVCKKLIFSMSSVIYDGAV-ACNCDPKGTQRDANGTLVCDPIGGQCK-CKSNVIGQRCDRCAPGSFGFSNK----GCSACDCDRIG-AIDNFCNILTGQ----CQCRNWASGRQCSLCPPNMWGFP----LCRPCRCYGHASSCNPKTGVCVDCQHNTVGKNCEACAVGFYGIATKGTPNDCKRCQCPGGSSGNKFSESCYVDTSTTPPSVLCDKCQVGYTGRQCEECADGYYGNPLVPGGSCKPCSCNNNIDPTVSGNCDRLTGSCLQCLYNTAGFNCERCKPGYFGDAIARNCTSCVCDKKGTNPGFHYSCNHKTGQCNCLPDVEGKQCDRCAE-GFWNLSSGKGCQQCDCCIEGSLRSMCNQITGQCQCKAGFGGPRCCECEDNFWGSPPDGCSACNCNPAGSVHLQCDRDTGKCLCKVGHIGDKCDMCAADT 1150 HSP 2 Score: 184.111 bits (466), Expect = 4.340e-46 Identity = 132/393 (33.59%), Postives = 190/393 (48.35%), Query Frame = 2
Query: 2690 CDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNY-----PN-CKKCLC----SPHALSADCDLNNGKC-------KCGSNVINRICDKCENLHYWNNRT---DCVPCNCGRG---SLNPKCDMETGNC-KCAQNVARRDCSKCDVGYFGLDV----TGC----KKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGT 3763
CDP+ G+C+C N +G C C G +G + K C CDCD +G+ C+ LTG+C CR RQC C ++ C PC C G SS CN +G C C N G CE C G++ PN CK+C C S + S C ++ KC R C++C + +Y N C PC+C +++ CD TG+C +C N A +C +C GYFG + T C K P ++ + G C C P+ G C++C+E W+ + GC++C+C I GS ++CN+I+G C CK F G +C C + ++G P C C+C G+ +CD+ TG+C CK IG KC +C T
Sbjct: 768 CDPIGGQCKCKSNVIGQRCDRCAPGSFGFS-NKGCSACDCDRIGAIDNFCNILTGQCQCRNWASGRQCSLCPPNMW-GFPLCRPCRC--YGHASS---CNPKTGVCVDCQHNTVGKNCEACAVGFYGIATKGTPNDCKRCQCPGGSSGNKFSESCYVDTSTTPPSVLCDKCQVGYTGRQCEECADGYYGNPLVPGGSCKPCSCNNNIDPTVSGNCDRLTGSCLQCLYNTAGFNCERCKPGYFGDAIARNCTSCVCDKKGTNPGFHYSCNHKTGQCNCLPDVEGKQCDRCAEGFWNLSSGKGCQQCDCCIEGSLRSMCNQITGQCQCKAGFGGPRCCECEDNFWGSPPDGCSACNCNPAGSVH---LQCDRDTGKCLCKVGHIGDKCDMCAADT 1150 HSP 3 Score: 123.25 bits (308), Expect = 1.390e-27 Identity = 83/241 (34.44%), Postives = 120/241 (49.79%), Query Frame = 2
Query: 2255 CPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNH-----SNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSC---KKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKC---NNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPC 2944
C G+ G CE C GY N ++ G C+ C+C N+ S CD L G C C +NTAG +CE C GY+GDA ++N SC KK P Y C+ + + C+ CL + G CDRC EG++ N C++C C + + C+ +TG+C+C G C C+ F+G + C C+C+ GS CD+ TGKC C+ + +CD C + K +C C
Sbjct: 931 CQVGYTGRQCEECADGYYG-NPLVPGGSCKPCSCNNNIDPTVSGNCDRLTGSCLQCLYNTAGFNCERCKPGYFGDAIARNCTSCVCDKKGTNPGFHYS----CNHKTGQ---CN-CLPDVEGKQCDRCAEGFW-NLSSGKGCQQCDCCIEGSLRSMCNQITGQCQCKAGFGGPRCCECEDNFWG-SPPDGCSACNCNPAGSVHLQCDRDTGKCLCKVGHIGDKCDMCAADTSGKMPQCEIC 1160 HSP 4 Score: 117.857 bits (294), Expect = 5.631e-26 Identity = 80/255 (31.37%), Postives = 118/255 (46.27%), Query Frame = 2
Query: 2999 GQCFCLENVNGDRCEKCVEGYFNYP----------NCKKCLCSPHAL---------SADCDLNNGKCK-CGSNVINRICDKCENLHY------WNNRTDCVPCNCG-RGSLN---------PKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVT---GCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNET-IGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEG-YYGFPN 3631
G+C C N G CE+C +GYF+ P C+KC C+ HA+ A +++ G C+ C N + R C++C+ LHY +++ C+ CNC G +N P + G C C +NV C +C G+F L GCK C P G+ + G C C G C++C+ + W + T GC C C + GSY N C+K +G C C+ G +C+R G YY P+
Sbjct: 281 GRCNCTHNTTGKNCERCQDGYFDVPWRPADADNPNECRKCNCNGHAVKCRFDPAVYQASGNVSGGVCQDCEHNTVGRNCERCKPLHYQDPSKKFSDPDACIACNCDPTGGVNGGECEAKNDPAAGLVAGRCICKRNVGGVRCDRCKAGFFNLTTDNPDGCKACGCNPLGTIGECNPDTGRCTCKRYVEGEDCDRCASEYWGLSSTPAGCSNCACDVGGSYDNQCDKQTGQCRCRKGITGRQCERVKTGNYYPLPD 535 HSP 5 Score: 91.2781 bits (225), Expect = 7.291e-18 Identity = 131/603 (21.72%), Postives = 235/603 (38.97%), Query Frame = 2
Query: 4589 GNRILINLKKCNCN------GYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSC----------YSGCTKDLLVRID-----TLNETVINFKWKT-----------LPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLE---------------DLRSTFTDINE----TISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASV----LKDVLKYVD-------ELNQIIKYTQS-----------------------HIQSDQINNLKILKNKTIMAD-EINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQD------------------EKDLISINVREIEDLMKNLSS-NHLVSKS---LIIPDTLKKHVEQQLNKS---SNLIKKLTKDFNFLHNAEQ-AKEAYSNITRDIL 6058
G+ I N C C+ G+ C+ TG C NC+ + EG +C C EGF+ N + C+ C C + C +I +CQC+ G+ G C C+ F+G+P G C +C+CN GS L C+ + G C C G +G KCD CA + + C+ C ++++ +ID TL T + + + LD + K N + + +EL + + ++K + + + EI +L+ N +L+ ++R F I E + +++ S + I AT + I+ P+ + L D++K ++ ELN I+ T + ++ D + K ++ M + + N KE + I++ M D Q + TK+ D L K I L+ +++ F D D I+ ++I + K+L+ + ++ K+ L + LK+ E+ K+ S++I+ +T + N +Q AK+A DI+
Sbjct: 1003 GDAIARNCTSCVCDKKGTNPGFHYSCNHKTGQC-NCLPDVEGKQCDRCAEGFW-NLSSGKGCQQCDC-CIEGSLRSMCNQITG----QCQCKAGFGGPRCCECEDNFWGSPPDG--CSACNCNPAGSVHLQCDRDTGKCLCKVGHIGDKCDMCAADTSGKMPQCEICGECYYQWKATLGFLSRNVTYQIDRAANLTLKTTGGAYAYDKELKELEEKLKRVQKILDAQKTKENDTKMIEAELDAATKRLREIKENTDRIANEVNSTAVKTHQANDEIARLRKLLNDLLQKADQMKNDILSVRDTNIRGAFEGIKENQRRSREAEKRVNGSLDSIDRATKNRKKIQMTLDGPPSFNQTHTKNQVDLGDLMKRIEELKRNSVELNGIVCGTPASKCGECTATNCSVCGGPGCNGTRNLAQDAVKRAKEAEDAQRMREAKANMTLKETMEAEMMVNMSRTAAETAISKAM-DAQMRAQNATKRMDQLIKDI--LEFLAQPFPDIDMSRMLANKVLNMTLSVSPDQITKLAQDIREAWKSLTGVDEILEKTKQNLTRAEDLKRRAEEAREKALTVSDVIQNVTDALDMAANGQQLAKDAIQLAEDDIV 1593 HSP 6 Score: 84.3445 bits (207), Expect = 8.862e-16 Identity = 58/166 (34.94%), Postives = 83/166 (50.00%), Query Frame = 2
Query: 4613 KKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFY--KNDKNYDQCEPCQCP--SAHYNFAQNC---TRIESSLELKCQCRIGYSGKSCENCQQGFYGNP-IKGIPCKSCDCNQ--QGSQSLNCNEF-GIC-QCSPGVLGIKCDQCAPNYVVE--KKTCQSC 5068
+ C C G++ C+ TG C +C N G C+ C GFY + C+ CQCP S+ F+++C T L +C++GY+G+ CE C G+YGNP + G CK C CN + S NC+ G C QC G C++C P Y + + C SC
Sbjct: 848 RPCRCYGHASSCNPKTGVCVDCQHNTVGKNCEACAVGFYGIATKGTPNDCKRCQCPGGSSGNKFSESCYVDTSTTPPSVLCDKCQVGYTGRQCEECADGYYGNPLVPGGSCKPCSCNNNIDPTVSGNCDRLTGSCLQCLYNTAGFNCERCKPGYFGDAIARNCTSC 1013 HSP 7 Score: 65.4698 bits (158), Expect = 4.456e-10 Identity = 60/202 (29.70%), Postives = 83/202 (41.09%), Query Frame = 2
Query: 3260 CDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNN----EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICA-CKTAFFGSKCDRCNEGYYGF-----PN-CQPCSC--AENGTKSNELTKCDKITGQC-----QCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
CD G CKC NV + C +C G FG GC C G + N G C C G C+ C + W F C+ C CY +++ CN +G+C C+ G C+ C G+YG PN C+ C C +G K +E D T +C+ G +C+ C G + + G C C C
Sbjct: 768 CDPIGGQCKCKSNVIGQRCDRCAPGSFGFSNKGCSACDCDRIGAIDNFCNILTGQCQCRNWASGRQCSLCPPNMWGFPL---CRPCRCY---GHASSCNPKTGVCVDCQHNTVGKNCEACAVGFYGIATKGTPNDCKRCQCPGGSSGNKFSESCYVDTSTTPPSVLCDKCQVGYTGRQCEECADGYYGNPLVPGGSCKPCSC 963 HSP 8 Score: 57.7658 bits (138), Expect = 9.976e-8 Identity = 56/209 (26.79%), Postives = 86/209 (41.15%), Query Frame = 2
Query: 4475 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC-----------DSVTGDCQNCMGNREGNKCQLCREGFYKNDK----NYDQCEPCQC-PSAHYNFAQNCTRIESSLEL---KCQCRIGYSGKSCENCQQGFYGNPIKGIP--CKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+C C T G +C+ GY+ + ++ R KCNCNG++ C + G CQ+C N G C+ C+ Y++ + D C C C P+ N + + + + L +C C+ G C+ C+ GF+ N P CK+C CN G+ + G C C V G CD+CA Y
Sbjct: 282 RCNCTHNTTGKNCERCQDGYFDVPWRPADADNPNECR----------KCNCNGHAVKCRFDPAVYQASGNVSGGVCQDCEHNTVGRNCERCKPLHYQDPSKKFSDPDACIACNCDPTGGVNGGECEAKNDPAAGLVAGRCICKRNVGGVRCDRCKAGFF-NLTTDNPDGCKACGCNPLGTIGECNPDTGRCTCKRYVEGEDCDRCASEY 479 HSP 9 Score: 51.6026 bits (122), Expect = 7.001e-6 Identity = 62/224 (27.68%), Postives = 93/224 (41.52%), Query Frame = 2
Query: 950 CICNNHADK-----CDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKV-HVNSD-DLCKTCKNG 1600
C CNN+ D CD++ + + C +NT G NCE C P + C C C++K ++ + +K G+ NC + EG C++C +GF WN+ + + C C C G++ S + +Q G C CK G+ G C C + P C C C+ GSV R C CKV H+ D+C +G
Sbjct: 961 CSCNNNIDPTVSGNCDRLTGSCL-----QCLYNTAGFNCERCKPGYFGDAI--ARNCTSCVCDKKGTNPGFHYSCN------HKTGQ-------CNCLPDVEGKQCDRCAEGF---WNLSSGK--GCQQCDCCIEGSLRS-------MCNQITGQCQCKAGFGGPRCCECEDNFWGSPPDGCSACNCNPAGSVHLQCDRDTGKCLCKVGHIGDKCDMCAADTSG 1152
BLAST of Laminin subunit alpha-2 vs. Ensembl Nematostella
Match: EDO31103 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYW2]) HSP 1 Score: 262.692 bits (670), Expect = 2.244e-70 Identity = 202/719 (28.09%), Postives = 313/719 (43.53%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIG-DQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKN-----------LKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYL--GNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDF--RLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYS---NQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVN-NVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTEL---CSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS--CKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGL--YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN 3067
+C+C+ + G C+ C P + + C+ C CN ++ S G+ E C + C +C+ ++ T C PC C+ L + D+ D G C CK G G C CS + C C C ++GS+ + C C+ + LC +C +G + L + +P+GC C+C G + CS S +DL GW G+ + F+S L ++ +Y++AP YL G + ++ F ++ +T S DVIIK F L S+ + + W+IG T + AT +E + L ++ L I+ ++ + L++IT++ D + ++E C CP + G CE C GY R N C C+C NH++TCD +G C +C HNT G HCE CA G YGDA + + C++CPCP + C S C C + +G C+RC +GYYG P ++ C++C CN N + +C+ TGEC +C YNT G CQ C GF+G+A K+C+ C+C GS C+ +TG+CDC C+ C Y K C C C QG S++ C+ G C C NV GD+C++C+ +++
Sbjct: 679 GQCSCRPHVIGRKCDECRPDYYD--FTSGLGCKACACNMTGSKRSSCHP---------DTGKCE-------CHPSITERQCSRCLDHYFN-----FTSGVGCQPCGCNELYS--------RDLQCDNDTGVCSCKDGVGGAKCTECSDAFYNLTTEGCTGCQCDLNGSLNPVCNKLNGNCTCRSNA-IGRLCDSCPDGSYGLRAGHPDGCLRCHCSGFSTNCSMASGYTRGQLISYLLLTADPTDLDGWTGRYPNGQLASNLIFSSISNLPGGFYRFYIEASSARDFYFSAPTKYLTRGRYAYTHAMTF----SLQQDNTTIPSSSTRGDVIIKGRYFNSELVTSLPTPPGLTFTTYKVVFLESNWRIGN-TSGRVATDKELVRVLSSLEYLWIRGRWTNLTDPSLKTRLMDITMTIANKSSIGDTVFNVETCQCPIQYVGQFCERCAPGYTRATPNGGAYTPCVPCSCNNHTDTCDPESGVCIDCQHNTTGDHCEKCAVGLYGDATNGTSSDCRECPCPGGPFSANSFARSCVLGSDGLPTCTNCSESHAGRQCERCQDGYYGTPTDPVNGQCRKCNCNGNVDPGVTGNCNTTTGECLKCLYNTSGFNCQWCVSGFHGNATAKTCQACNCTDRGSKHGECNNVTGQCDCHPLVTGLSCERCVKDAFNYTSGKGCTACGCHPQG--SNDTQCDAVFGNCTCNTNVKGDKCDQCMPNFYD 1358 HSP 2 Score: 144.821 bits (364), Expect = 3.539e-34 Identity = 160/659 (24.28%), Postives = 246/659 (37.33%), Query Frame = 2
Query: 3242 GSLNPK-CDMETGNCKCAQNVARRDCSKCDVGYFGLDV-TGCKKCPPCYNGQ---VCN-NEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP--NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNH-----------------------RIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWP-----LTMFRPIYVNVMKLFNSHD-LSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKN--YDQCEPCQCPSAHY---NFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGI--PCKSCDCNQQGSQSL--NCN-EFGIC-QCSPGVLGIKCDQCAPNYV--VEKKTCQSC 5068
G++ P CD G C C +V R C +C Y+ GCK C G C+ + G C C P+ C++C + ++F +GC+ C C S C+ +G+C+CK G+KC C++ +Y C C C NG+ + C+K+ G C C+ N IG C C G++ + GC C C G S C + R + IF ++L G + ++ A+ R F + LT R Y + M D ++ S +V + GR + + + GL Y V F E + W I + + L R + + L + L + L+ I + + +IG V VE C C G C+ GY + +T +G T C+CN ++ CD +G C +C N G+ C+ C G Y + N C C CP + +FA++C L C ++G+ CE CQ G+YG P + C+ C+CN + NCN G C +C G C C + KTCQ+C
Sbjct: 667 GAVTPSVCDQCGGQCSCRPHVIGRKCDECRPDYYDFTSGLGCKACACNMTGSKRSSCHPDTGKCECHPSITERQCSRCLDHYFNFTSGVGCQPCGCNELYSRDLQCDNDTGVCSCKDGVGGAKCTECSDAFYNLTTEGCTGCQCDLNGSLN---PVCNKLNGNCTCRSNAIGRLCDSCPDGSYGLRAGHPDGCLRCHCSGFSTNCSMASGYTRGQLISYLLLTADPTDLDGWTGRYPNGQLASNLIFSSISNLPGG--FYRFYIEASSARDFYFSAPTKYLTRGRYAYTHAMTFSLQQDNTTIPSSSTRGDVIIK------GRYFNSELVTSLPTPPGLTFTTYKVV------------FLESN----WRIGNTSGRV-----------ATDKELVRVLSSLEYLWIRGRWTNLTDPSLKTRLMDITM----TIANKSSIGDTVFNVETCQCPIQYVGQFCERCAPGYTR-------ATPNGGAYT------PCVPCSCNNHTDTCDPESGVCIDCQHNTTGDHCEKCAVGLYGDATNGTSSDCRECPCPGGPFSANSFARSCVLGSDGLPTCTNCSESHAGRQCERCQDGYYGTPTDPVNGQCRKCNCNGNVDPGVTGNCNTTTGECLKCLYNTSGFNCQWCVSGFHGNATAKTCQAC 1270 HSP 3 Score: 109.383 bits (272), Expect = 1.813e-23 Identity = 93/327 (28.44%), Postives = 143/327 (43.73%), Query Frame = 2
Query: 317 VCDNRNETKRHPIEFAKSDDD-NLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLK---MGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAA-DLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYR 1279
VCD N HP+ D +W+S +NV+ + + + VYI ++ + + P L +S D G Y F++ + YN+ + QY N + +SL E F S + +N + ++I + I+ + FL + R K++T D T L++S +Y+S++NI+I C CN AD CD + C C+ NT G+NCE C PLFN +P+ P CE+C CN S C Y+ + +G GVC+NC +NT G CE+C FYR
Sbjct: 69 VCDASNPNTSHPVSHVNDAFDLKTFWKS---------RNVDQPVFIDFNFGAVYIFMQAMLLFEYELPAAMYLSKSKDFGSSYDVIGFYSSDCQAYYNMTETQY-------NRRNASLT--ECFKINPLSSDQRVNHMPMQDTVIAAK----------IRVDHNVQLKFLMTNF-RVVLDKYVTPPGYDPPTSSDTLKKS----FYFSIQNIDILAACFCNGQADTCDTK-----DPSVCVCEKNTAGNNCEKCLPLFNNKPYAPQRACEDCGCNNHSASCEYHSS--------------KGYGVCLNCTDNTMGDKCEQCKPEFYR 343 HSP 4 Score: 96.6709 bits (239), Expect = 1.490e-19 Identity = 72/234 (30.77%), Postives = 109/234 (46.58%), Query Frame = 2
Query: 2615 IEGYYGNPKKKISCKRCKCNNN----QESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNC-EIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP--NCKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHY---WNNRTDCVPCNCGRGSLNPKCDMETG 3277
+ G+YG+ +K + C+CN CD G+C C + +G +C C+ +Y CK C C+ GS +C TGKC+C + RQC C + C PC C E+ R + C+ +G C C + V G +C +C + ++N C C C + +L+ C+ NG C C SN I R+CD C + Y + C+ C+C S N C M +G
Sbjct: 647 LAGFYGDLYQKG--QACECNATGAVTPSVCDQCGGQCSCRPHVIGRKCDECRPDYYDFTSGLGCKACACNMTGSKRSSCHPDTGKCECHPSITERQCSRCLDHYFNFTSGVGCQPCGCNELYSR---DLQCDNDTGVCSCKDGVGGAKCTECSDAFYNLTTEGCTGCQCDLNGSLNPVCNKLNGNCTCRSNAIGRLCDSCPDGSYGLRAGHPDGCLRCHCSGFSTN--CSMASG 873 HSP 5 Score: 95.1301 bits (235), Expect = 4.343e-19 Identity = 64/186 (34.41%), Postives = 91/186 (48.92%), Query Frame = 2
Query: 3119 CDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC-------YNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSC 3649
CD G+C C +VI R CD+C +Y + + C C C GS C +TG C+C ++ R CS+C YF + T C PC + Q N+ G+C C G C +CS+ ++ T GC C C +NGS + +CNK++G C C++ G CD C +G YG P C
Sbjct: 674 CDQCGGQCSCRPHVIGRKCDECRPDYYDFTSGLGCKACACNMTGSKRSSCHPDTGKCECHPSITERQCSRCLDHYF--NFTSGVGCQPCGCNELYSRDLQCDNDTGVCSCKDGVGGAKCTECSDAFYNLT-TEGCTGCQCDLNGSLNPVCNKLNGNCTCRSNAIGRLCDSCPDGSYGLRAGHPDGC 856 HSP 6 Score: 92.0485 bits (227), Expect = 3.448e-18 Identity = 88/308 (28.57%), Postives = 129/308 (41.88%), Query Frame = 2
Query: 2813 SLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFN------YPNCKKCLCSPHALSAD-----CDLNNGK----CKCGSNVINRICDKCENLHYWNNRTD-----CVPCNCGRGSLNP----KCDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTGCKKCPPC-------YNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY 3619
S+G T N + C C +V + C+ C G C+PC+C + C+ SG C C N GD CEKC G + +C++C C SA+ C L + C + R C++C++ Y+ TD C CNC G+++P C+ TG C KC N + +C C G+ G K C C +G+ N G C C P G C +C +D++++ GC C C+ GS C+ + G C C T G KCD+C +Y
Sbjct: 1063 SIGDTVFNVET----CQCPIQYVGQFCERCAPGYTRATPNGGAYTPCVPCSCN-----NHTDTCDPESGVCIDCQHNTTGDHCEKCAVGLYGDATNGTSSDCRECPCPGGPFSANSFARSCVLGSDGLPTCTNCSESHAGRQCERCQD-GYYGTPTDPVNGQCRKCNC-NGNVDPGVTGNCNTTTGECLKCLYNTSGFNCQWCVSGFHGNATA--KTCQACNCTDRGSKHGECNNVTGQCDCHPLVTGLSCERCVKDAFNYTSGKGCTACGCHPQGSNDTQCDAVFGNCTCNTNVKGDKCDQCMPNFY 1357 HSP 7 Score: 77.7962 bits (190), Expect = 7.112e-14 Identity = 54/155 (34.84%), Postives = 74/155 (47.74%), Query Frame = 2
Query: 4613 KKCNCNG-----YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTC 5059
+KCNCNG + C++ TG+C C+ N G CQ C GF+ N C+ C C + C + +C C +G SCE C + + N G C +C C+ QGS C+ FG C C+ V G KCDQC PN+ K+C
Sbjct: 1216 RKCNCNGNVDPGVTGNCNTTTGECLKCLYNTSGFNCQWCVSGFHGN-ATAKTCQACNCTDRGSKHGE-CNNVTG----QCDCHPLVTGLSCERCVKDAF-NYTSGKGCTACGCHPQGSNDTQCDAVFGNCTCNTNVKGDKCDQCMPNFYDVGKSC 1363 HSP 8 Score: 73.1738 bits (178), Expect = 1.801e-12 Identity = 78/266 (29.32%), Postives = 112/266 (42.11%), Query Frame = 2
Query: 3113 ADCDLNNGKCKCGSNVINRICDKCENLHY-----WNNRTDCVPCNCGRGSLNPKCDMETGNC-KCAQNVARRDCSKCDVGYFGLDVTG----CKKCPPCYNGQ----------VCNNEGICVC---PPNTIGNLCNKCSEDSWDFNETIG----CKKCNCY--INGSYSNICNKISGIC-ACKTAFFGSKCDRCNEGYYG---FPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
D N C+C + + C++C + T CVPC+C + CD E+G C C N C KC VG +G G C++CP C G V ++G+ C + G C +C +D + T C+KCNC ++ + CN +G C C G C C G++G CQ C+C + G+K E C+ +TGQC C V G C+ C K F + T GC +C C
Sbjct: 1065 GDTVFNVETCQCPIQYVGQFCERCAPGYTRATPNGGAYTPCVPCSCNNHT--DTCDPESGVCIDCQHNTTGDHCEKCAVGLYGDATNGTSSDCRECP-CPGGPFSANSFARSCVLGSDGLPTCTNCSESHAGRQCERC-QDGYYGTPTDPVNGQCRKCNCNGNVDPGVTGNCNTTTGECLKCLYNTSGFNCQWCVSGFHGNATAKTCQACNCTDRGSKHGE---CNNVTGQCDCHPLVTGLSCERCVKDAF--NYTSGKGCTACGC 1321 HSP 9 Score: 58.151 bits (139), Expect = 7.488e-8 Identity = 62/231 (26.84%), Postives = 94/231 (40.69%), Query Frame = 2
Query: 2240 IEKCNCPPGFNGLSCESC--ESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENY-VCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY 2917
++K PPG++ + +S Y + N+ AC C ++TCD+ + C NTAG +CE C + + +C+ C C + C +SS+ Y VC C NT G C++C +Y NP ++ C + + T + K GFY T C C+CD GS + C G C CR +F +CD G Y
Sbjct: 218 LDKYVTPPGYDPPTSSDTLKKSFYFSIQNIDILA---ACFCNGQADTCDTKDPSVCVCEKNTAGNNCEKCLP-LFNNKPYAPQRACEDCGC----NNHSASCEYHSSKGYGVCLNCTDNTMGDKCEQCKPEFYRNPDTPLNHTN-TCLGKTNHIMFINSIINQTKGFRPTRENIKKDGFYSLNAGNTAGCSACNCDPAGSINVTCSD-GGACHCRPHFSGLKCDVIERGFY 438 HSP 10 Score: 52.7582 bits (125), Expect = 2.834e-6 Identity = 49/165 (29.70%), Postives = 71/165 (43.03%), Query Frame = 2
Query: 4613 KKCNCNGYS----KVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNC-NEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDL 5092
+ C CN VCD G C +C + G KC CR +Y C+ C C N + KC+C + + C C ++ N G+ C+ C CN+ S+ L C N+ G+C C GV G KC +C+ + T + C +GC DL
Sbjct: 659 QACECNATGAVTPSVCDQCGGQC-SCRPHVIGRKCDECRPDYYDFTSGLG-CKACAC-----NMTGSKRSSCHPDTGKCECHPSITERQCSRCLDHYF-NFTSGVGCQPCGCNELYSRDLQCDNDTGVCSCKDGVGGAKCTECSDAFY--NLTTEGC-TGCQCDL 812 HSP 11 Score: 52.373 bits (124), Expect = 4.775e-6 Identity = 41/143 (28.67%), Postives = 63/143 (44.06%), Query Frame = 2
Query: 4613 KKCNCNGYSKVCDSVT----GDCQNCMGNREGNKCQLCREGFYKN-DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQ-GFYG-NPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCD 5020
+ C CN +S C+ + G C NC N G+KC+ C+ FY+N D + C + H F + + EN ++ GFY N C +C+C+ GS ++ C++ G C C P G+KCD
Sbjct: 300 EDCGCNNHSASCEYHSSKGYGVCLNCTDNTMGDKCEQCKPEFYRNPDTPLNHTNTCLGKTNHIMFINSIINQTKGFR-----------PTRENIKKDGFYSLNAGNTAGCSACNCDPAGSINVTCSDGGACHCRPHFSGLKCD 431
BLAST of Laminin subunit alpha-2 vs. Ensembl Nematostella
Match: EDO36501 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF6]) HSP 1 Score: 243.817 bits (621), Expect = 2.779e-64 Identity = 262/1045 (25.07%), Postives = 425/1045 (40.67%), Query Frame = 2
Query: 92 MR-IFIIFILLIVHKAHSQ-------DDEPEILSVNVDGLLMENAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAKS---DDDNLWWQSPSMAQGLKYQ-----NVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRF----LKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV------CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQC--HGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCS---IGYERYPNGECLPCPCSIDGSVRTSFY--RCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC-FG--ITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFT--SFQ-------YLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEF-----------ISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNV------------------LYQGQ----CEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHK------CLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQES---CDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKK----CIPCNC 2950
MR + ++ LL V K +S+ DD + M++ ++S++TCG+ P +CR+ + +N C C+ + T+RHPIE+ D +WQS + + VNV+I + Y I ++ +SPRP ILE+S D G+ + P +++ ++C F + T+ + D CT YS +G++ + Y + +LSA +R R L +A D +T K +YYY++ ++E+ +C C+ HA CD E G C+C HNT G +CE C PLFN + W P CE C C+ ++ C+Y+Q EG G C +C NTEG CE+C FYR ++ C C C G+ G+C +Q G C C+ G CD C+ GY P G C C C +G+V SF + C+CK +V++ C+ CK+GY++ C+ C C +G + CS + + +++ G + T T P F+ +F+ Y+ + N+ + F + T Y P Y+ N+ +++ + + G+ DS + I V++ + + + W+ TP + + ++ + VD L+I L ++T SR A + + H GF+ + C + + ++ + ++ G C++ Y +S TCD G+CQ C G C+ C G+Y N S PC C S S + VC+ C C RC + +YG C C+CN + + CD VTG C C +T G +C C+ GFY + + C+ C+C GS S+ C+ TG C C++N + +C+SC G Y D C PC C
Sbjct: 1 MRGLGMVLFLLTVSKVNSECFENEKYDDVQTCVPEPRFAAKMKHRAVSSTSTCGSP-PSEYCRV-------KPLNG-----CFKCNASSPTERHPIEYIADKSPGSDATFWQSETWWDWYQKNPRDDLKVNVSISFNKTYDITGHIMFTFESPRPSKAILEKSFDKGQTWDPLQYYS---FNCRKDFGME------NTDPTENRFGDFSAKCTEKYSSSSPQQNGKVTFDFL--------RRYNKASFFDTQVQQYLSATDLRLRMEYPGTDGLEVAKDEVTLK---------QYYYAVSDLEVIARCKCHGHARWCDFPHETG---KNCDCLHNTTGDDCERCLPLFNNKTWMPATSETEPNPCERCECHSHADSCTYSQ--------------QEGYGTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNCFPQGITNNGTC--------NQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVGNGTVNASFNCDQYNGQCSCKPNVHT-RTCQECKHGYWDFPKTLDTDCKRCDCDYGGSVLQICSKTTGSCMCRTNIIGTRCTDVTTGYFT-PSFSYLTFEAEEATGDYMQASNTLDLDMFFSGTGYGRITPGQYI-NFQARVDVNWKWFLMLRYTDLATGA--DSLQIHISAEAAGNCSGVNMT--VMNVTGLLAGQGLAWRAA--TPIVMCPGQTYNFNVTFSPGSSVNASIDVDSLVI------------LPDVTESRVYKVAAAEGVAH--------GFDSDTIRQCWNNWTTISTIVTSQPICSKNISFSVMSEVFHGAMACGCDSVGTYLNS-TCDRYGGKCQ-CKPGVTGRTCDKCKPGFY------NFTSSGCAPCD---------CVSTGSHHLVCNAVSGQCPCKPGVITRTCSRCNDSFYGYTTGS-GCVECRCNMSGSADAQCDDVTGNCTCKNSTDGAKCAECRFGFY-NFTSAGCQACNCHEGGSQSQQCNLQTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYC 933 HSP 2 Score: 157.147 bits (396), Expect = 6.869e-38 Identity = 149/595 (25.04%), Postives = 233/595 (39.16%), Query Frame = 2
Query: 3248 LNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQ---VCNN-EGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWP-----LTMFRPIYVNVMKLFNSHDLSLFYRSIF----MNVSVDCLDNKC--GREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTF-GNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREG-------------FYKNDKNYDQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKG-IPCKSC 4930
LN CD G C+C V R C KC G++ +GC C G VCN G C C P I C++C++ + + GC +C C ++GS C+ ++G C CK + G+KC C G+Y F CQ C+C E G++S + C+ TG C CK N +G KC+ CR GT+ SD GC C+CFG S C + SG S I ++ P W ++M R S +++L + F +N + L+ K + P ++F E G+ + H NG W + + D +L D I N M+ +V ++KL++ + +G++ VE C C + + G C+ G+ + N PN++ S C C G S CD TG C+ C +G C+ C+ G ++ + + C+ C C P+ Y + C +C C+ SG++C+ C + Y +G + C C
Sbjct: 743 LNSTCDRYGGKCQCKPGVTGRTCDKCKPGFYNFTSSGCAPCDCVSTGSHHLVCNAVSGQCPCKPGVITRTCSRCNDSFYGYTTGSGCVECRCNMSGSADAQCDDVTGNCTCKNSTDGAKCAECRFGFYNFTSAGCQACNCHEGGSQSQQ---CNLQTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYCFGRSNTCTSA-----------------------SGFVRSYIKVDLGSPFSIDWTFVQRDVSMARVSQGRNFSFVTSGNMTLGTKVNFTKNQLNSYGELLEMKLKYSSQGPLNVKMFWELEKTNGGIVRFDVKPTPSPEVTSYYVRLHEANGIWKTEPNRRNASDLQELLSDLMSITLNAQ----FMSNGSVLLSEIKLHS------------------ASQGIVGEKAGHVENCTCESGNYTGLSCELCNQGFTR---NRPNNSYSA-----------CSLCRCTGRSLDCDGETGQCKGCRLGSQGLYCEQCQPGVDNTTDCTQCLPEYWGLKEGSNGCQDCDCVLPNTLYGNSTVCNATTG----QCTCKEKISGRTCDRCHENAYNTSDRGCVECPEC 1271 HSP 3 Score: 126.716 bits (317), Expect = 1.241e-28 Identity = 144/582 (24.74%), Postives = 230/582 (39.52%), Query Frame = 2
Query: 1010 AKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSA---KNLKISDLKGWKLT-TFTGEKIALPKFT---SFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSL---IDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWK-IGKLTPDK--AATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPG-FNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASG-------------YYGDAKSKNLNSCKKCPC--PRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK-ISCKRC 2665
KC CK G C+ C P F + C C+C + + L N + G C C+ C +C FY T C C+C+ GS + +D+ G+C CK +G C C G+ + + C C C GS C CK + + C++C+ G ++ D NP+GC PCYCFG +N C+S S +K+ + + TF +++ + + +F ++TS N + + N + N + SYG + +K Q L + ++ + I D + S + Y++ E I K P++ A+ +E +S L+ + + A++ SN S+ L I L + ++ H+E C C G + GLSCE C G+ R C C C S CD G+C+ C + G +CE C G Y+G + N C+ C C P Y + +C++ + + C C SG CDRC E Y + + C C
Sbjct: 752 GKCQCKPGVTGRTCDKCKPGFYNFT---SSGCAPCDC----------VSTGSHHLVCNAVS-----GQCP-CKPGVITRTCSRCNDSFYG-----YTTGSGCVECRCN---MSGSADAQCDDV----TGNCTCKNSTDGAKCAECRFGFYNFTSAGCQACNCHEGGSQSQQCNLQTGICTCKSNALGEK-CESCRPGTYHSDPNNPDGCTPCYCFGRSNTCTSASGFVRSYIKVDLGSPFSIDWTFVQRDVSMARVSQGRNFSFVTSGNMTLGTKVN------------FTKNQLNSYGELLEMKLKYSSQGPLNVKMFWELEKTNGGIVRFDVKPTPSPEVTS--------YYVRLHEANGIWKTEPNRRNASDLQELLSDLMSI---TLNAQFMSN-GSVLLSEIKLHSASQGIVGEKAGHVENCTCESGNYTGLSCELCNQGFTRNRPNNSYSACSLCRCTGRSLDCDGETGQCKGCRLGSQGLYCEQCQPGVDNTTDCTQCLPEYWGLKEGS--NGCQDCDCVLPNTLYGNSTVCNATTGQ---C-TCKEKISGRTCDRCHENAYNTSDRGCVECPEC 1271 HSP 4 Score: 121.709 bits (304), Expect = 4.317e-27 Identity = 209/922 (22.67%), Postives = 330/922 (35.79%), Query Frame = 2
Query: 2936 IPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN----------YPN-CKKCLCSPHALSADCDLNNGKCKCGS---NVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFN-ETIG-CKKCNCYINGSY--SNICNKISGICACKTAFFGSKCDRCNEGYYGFP-----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTF-PSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLF---GMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILED-----------SNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQA-----IGKRVTAV-----EKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNC---NGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCN-EFGICQCSPGVLGIKCDQCAPNYVVEKKT----CQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAY---LETIKNIYKEMPNITLAASVLKDVL 5524
+ C+ G F + T C CL N GD CE+C+ + N PN C++C C HA S G C N C++C++ Y N + + + P C NC F +T NG G C C N G C+ C++ W + E IG C +C C NG+ S C++ +G C+CK C C GY+ FP +C+ C C G+ L C K TG C C+ N+IGT+C G F PS FS F E A ++ N++ + +F I+ A + W L + DL+ S+ +++S + N G + + + GL G+ + + +FN G V + +D +IL D + + D N T++ + + + I +++ +V A +G + + KC C G C G+Y N T SG C+C + VC++V+G C C C C + FY C C+C + AQ C + + C C+ G C C+ GFY G C++C+C++ GSQS CN + GIC C LG KC+ C P C CY + + I + P ++D + ++S+ S+ N F N + F K + ++ YGE+ ++K +Y+ F ++ +T G + + T+Y L I+K PN AS L+++L
Sbjct: 265 VIARCKCHGHARWCDFPHETGKNCDCLHNTTGDDCERCLPLFNNKTWMPATSETEPNPCERCECHSHADSCTYSQQEGYGTCDDCYHNTEGHFCERCKDKFYRN-----MSLSLNHPEICPDC-----NC------------------FPQGITN--------NGTCNQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVGNGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHGYWDFPKTLDTDCKRCDCDYGGSV---LQICSKTTGSCMCRTNIIGTRCTDVTTGYFTPS--------FSYLTFEAEE---ATGDYMQASNTL-DLDMFFSGTGYGRITPGQYINFQARVDVNWKWFL------------MLRYTDLATGADSLQIHISAEAAGNCSG------VNMTVMNVTGLLAGQGLAWRAATPIVMCPGQTYNFNVTFSPGSSVNASID---VDSLVILPDVTESRVYKVAAAEGVAHGFDSDTIRQCWNNWTTISTIVTSQPICSKNISFSVMS-EVFHGAMACGCDSVGTYLNSTCDRYGGKCQCKPGVTGRTCDKCKPGFY-------NFTSSG-----------CAPCDCVSTGSHHLVCNAVSGQCP-CKPGVITRTCSRCNDSFYGYTTG-SGCVECRCNMSGSADAQ-CDDVTGN----CTCKNSTDGAKCAECRFGFYNFTSAG--CQACNCHEGGSQSQQCNLQTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYCFGRSNTCTSASGFVRSYIKVDLGS-PFSIDWTFVQRDVSMARVSQGRNF--SFVTSGNMTLGTKVNFTKNQLNS---YGELLEMKLKYSSQGPLNVKMFWELEKTNGGIVRFDVKPTPSPEVTSYYVRLHEANGIWKTEPN-RRNASDLQELL 1079 HSP 5 Score: 100.908 bits (250), Expect = 7.707e-21 Identity = 61/191 (31.94%), Postives = 90/191 (47.12%), Query Frame = 2
Query: 2687 SCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--PNCKKCLC-SPHALSADCDLNNGKCKCGSNVINRICDKCENLHYW---NNRTDCVPCNC 3235
+CD G+C+C G C CK GFY + + C CDC S GS C+ ++G+C C+ + R C C Y C+ C C + G S++ C+ +G C C + +G +C +C G++N+ C+ C C + S C+L G C C SN + C+ C Y NN C PC C
Sbjct: 746 TCDRYGGKCQCKPGVTGRTCDKCKPGFY-NFTSSGCAPCDCVSTGSHHLVCNAVSGQCPCKPGVITRTCSRCNDSFYGYTTGSGCVECRCNMSG--SADAQCDDVTGNCTCKNSTDGAKCAECRFGFYNFTSAGCQACNCHEGGSQSQQCNLQTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYC 933 HSP 6 Score: 98.2117 bits (243), Expect = 5.066e-20 Identity = 71/228 (31.14%), Postives = 112/228 (49.12%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGY-------YGNPKKKISCKRCKCNNNQESC----DPVTGECR-CSYNTMGTECQLCKHGFYGDAL-----TKSCKKCDCDSLGSTSEN-CDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKK----CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCS-PHALSADCDLNNGKCKCGSNVINRIC 3175
CLHNT+G C+RC+ + + + C+RC+C+++ +SC G C C +NT G C+ CK FY + + C C+C G T+ C++ TG+C+CR N RQCDSC + + C C C G +++ C+ +GQC C NV+ C++C GY+++P +CK+C C ++ C G C C +N+I C
Sbjct: 290 CLHNTTGDDCERCLPLFNNKTWMPATSETEPNPCERCECHSHADSCTYSQQEGYGTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNCFPQGITNNGTCNQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVGNGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHGYWDFPKTLDTDCKRCDCDYGGSVLQICSKTTGSCMCRTNIIGTRC 517 HSP 7 Score: 87.0409 bits (214), Expect = 1.226e-16 Identity = 64/217 (29.49%), Postives = 90/217 (41.47%), Query Frame = 2
Query: 2471 SKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKC---NNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLY-LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEG-YFNYPN----CKKCLC 3094
S+ + C C + + C Y + C +C +G CD+C G+Y C C C ++ C+ V+G+C C + C C FYG C +C C+ GS CD +TG C C+ + +C C G Y C CNC G S + CN +G C C N G++CE C G Y + PN C C C
Sbjct: 725 SEVFHGAMACGCDSVGTYLNSTCDRYGGK---C-QCKPGVTGRTCDKCKPGFYNFTSS--GCAPCDCVSTGSHHLVCNAVSGQCPCKPGVITRTCSRCNDSFYGYTTGSGCVECRCNMSGSADAQCDDVTGNCTCKNSTDGAKCAECRFGFYNFTSAGCQACNCHEGGSQSQQ--CNLQTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYC 933 HSP 8 Score: 56.225 bits (134), Expect = 2.755e-7 Identity = 99/476 (20.80%), Postives = 177/476 (37.18%), Query Frame = 2
Query: 4661 GDCQNCM---GNREGNKCQLCREGFYKNDKN--YDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQG-------------FYGNPIKGIPCKSCDC---NQQGSQSLNCNEF-GICQCSPGVLGIKCDQCAPN-YVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPS--------TLDLRLNKINISIQ-MSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEK-LKTRYNKVLEDLRS-TFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVL-KYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQK-LDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKK 5980
G +NC GN G C+LC +GF +N N Y C C+C + + + CR+G G CE CQ G ++G C+ CDC N S CN G C C + G CD+C N Y + C C C + L + L + + + + L T L N+S++ +SS NS L E+ K+ D K E+E+ LK R L L T T E + + + ++ L+++ + + + N T + LK+++ ++ + + + QS Q+ N+ + +KT A + T+ ++ ++ + ++ + ++++ + L + LD S++ Q L N + + + D K + + + SNL K+
Sbjct: 1117 GHVENCTCESGNYTGLSCELCNQGFTRNRPNNSYSACSLCRCTGRSLDCDGETGQCKG-------CRLGSQGLYCEQCQPGVDNTTDCTQCLPEYWGLKEGSNGCQDCDCVLPNTLYGNSTVCNATTGQCTCKEKISGRTCDRCHENAYNTSDRGCVECPE-CFRQLQEHVFKLRDGIKDLQGLILRVKNGDIGDITFAQSLAAANVSVEAISSNAKNSSGIEGTLHQELL-------KLNDTLK----ELERVLKGRVTSELTSLEGVTMTTQQERVQIQDHIRLTEGLVRSGYGVVTSTMQAAVDQANAT--DTYLKELVPRFTSQAS--MATNQSARQNGTGND---ITSKTSSAGALVTSADVTSLEAGLRQNMTSQLLVALEQQAQSVRRLATETNATAHALYANVTAVLDGASRALQAANGLGPDNAAAVLSVKR-------------AADDAKANASSAVQRVSNLTKQ 1553
BLAST of Laminin subunit alpha-2 vs. Ensembl Nematostella
Match: EDO46760 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNY3]) HSP 1 Score: 212.231 bits (539), Expect = 1.973e-54 Identity = 178/560 (31.79%), Postives = 247/560 (44.11%), Query Frame = 2
Query: 2303 YRRVNNVLYQGQCEACACYNHSNTCDSLNG----------ECQNCTHNTAGAHCEDCASGYYGDAKSKNLN----SCKKCPCPRLD---YQMTELCSSYSSENY----VCHKCLHNTSGVHCDRCIEGYYGNPKKKIS----CKRCKCNN------------NQESCDPVTGECRCSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSEN--CDKLTGKCDCRTNFVNRQCDSCTSGLY-LKD---KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGY--FNYPNCKKCLCSP----HALSADCDLNNGKCKCGSNVINRICDKCENLHY---WNNRTDCVPCNC---GRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVT---GCKKC------------PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP-NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGT 3763
Y + VL G+CE C+ H+ + G EC T T G CE C Y A + N C KC C Y + L S +S VC CLHNT+G C C + ++ P K +S C C+C + + + G+C C T G +C CK GFY + + C C C++ G+ + N CD+ G+C C++N R CD+C SG Y L+D C C C+ G + C+ SGQC C G RC C +GY + CK C CS L CD NG+C C + V + CD+C+ +Y N+ C C+C G + C++ +GNC C + V C +C GYF L + GC+ C P + + G C C N G C++C + GC CNC +GS + CN +G C CK G +CDRC G YG P +C+PC+C G+ S+ CD +G+C CK NV G KC C+ GT
Sbjct: 487 YFALKRVLVTGRCE---CHGHAQKVTFIKGNSSSPGRCRCECDPSTF-TQGEKCESCLPMYNNKAFLRGTNDDPYPCVKCECNNHATACYYNSSLDPSPASRTVGGGGVCINCLHNTAGQFCHVCRDDFFREPGKSLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAGTVNRNASCDQSRGQCYCKSNVTGRTCDTCESGFYGLRDNDPSGCRACECDPFGTLPG-SVCDAGSGQCRCKAGSTGLRCHLCEDGYHSLDSSGCKTCNCSTIGAKPGLVNTCDKTNGQCYCKTYVEGKTCDRCKTGYYNLTTVNQDGCSLCSCDPKGIQGSSLTCEVSSGNCTCKKYVREPTCGQCMKGYFNLSSSNPEGCQACNCNPKGTLGGDRTPAGELECSGSNGKCACLTNVQGLRCDQCVSAYYWNPSGYGCSPCNCDASGSLATNCNS-TGYCQCKPNIGGRRCDRCMPGSYGGPGSCKPCNCNMAGSLSD---TCDD-SGKCNCKPNVQGDKCDTCKDGT 1036 HSP 2 Score: 182.185 bits (461), Expect = 2.120e-45 Identity = 163/520 (31.35%), Postives = 223/520 (42.88%), Query Frame = 2
Query: 2246 KCNCPPGF--NGLSCESCESGY------RRVNNVLYQGQCEACACYNHSNTC---DSLN-----------GECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKC-PC----PRLDYQMTELCSSYSSENYVCHKC--LHNTSGVHCDRCIEGYYG-NPKKKISCKRCKCN-----NNQESCDPVTGECRCSYNTMGTECQLCKHGFYG--DALTKSCKKCDCDSLGSTSEN-CDKLTGKCDCRTNFVNRQCDSCTSGLY-LKDKKCIPCNCE-IQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP-----NCKKCLCSPHAL---SADCDLNNGKCKCGSNVINRICDKCENLHY---WNNRTDCVPCNCG-RGSL----NPKCDME----TGNCKCAQNVARRDCSKCDVGYF-GLDVTGCKKCPPCYNGQV---CNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEG 3613
+C C P G CESC Y R N+ Y C C C NH+ C SL+ G C NC HNTAG C C ++ + K+L++ C PC P ++ T S+ + +C T G CD C G+Y C C CN N SCD G+C C N G C C+ GFYG D C+ C+CD G+ + CD +G+C C+ +C C G + L C CNC I + C+ T+GQC+C V G C++C GY+N C C C P + S C++++G C C V C +C ++ +N C CNC +G+L P ++E G C C NV C +C Y+ GC C +G + CN+ G C C PN G C++C S+ + CK CNC + GS S+ C+ SG C CK G KCD C +G
Sbjct: 522 RCECDPSTFTQGEKCESCLPMYNNKAFLRGTNDDPYP--CVKCECNNHATACYYNSSLDPSPASRTVGGGGVCINCLHNTAGQFCHVCRDDFFRE-PGKSLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAGTVNRNASCDQSRGQCYCKSNVTGRTCDTCESGFYGLRDNDPSGCRACECDPFGTLPGSVCDAGSGQCRCKAGSTGLRCHLCEDGYHSLDSSGCKTCNCSTIGAKPGLVNTCDKTNGQCYCKTYVEGKTCDRCKTGYYNLTTVNQDGCSLCSCDPKGIQGSSLTCEVSSGNCTCKKYVREPTCGQCMKGYFNLSSSNPEGCQACNCNPKGTLGGDRTPAGELECSGSNGKCACLTNVQGLRCDQCVSAYYWNPSGYGCSPCNCDASGSLATNCNSTGYCQCKPNIGGRRCDRCMPGSYGGPGS--CKPCNCNMAGSLSDTCDD-SGKCNCKPNVQGDKCDTCKDG 1035 HSP 3 Score: 158.688 bits (400), Expect = 3.265e-38 Identity = 135/470 (28.72%), Postives = 203/470 (43.19%), Query Frame = 2
Query: 2588 TSGVHCDRCIEGY------YGNPKKKISCKRCKCNNNQESC------DPVT--------GEC-RCSYNTMGTECQLCKHGFY---GDALTKS--CKKCDCDSLG--STSENCDK-------LTGKCDCRTNFVNRQCDSCTSGLY-LKDKK---CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYF----NYPN-CKKCLCSPHAL--SADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNP----KCDMETGNCKCAQNVARRDCSKCDVGYFGLDVT---GCKKC---PPCYNGQVCN---NEGICVCPPNTIGNLCNKCSEDSWDFNET--IGCKKCNCYINGSYSNI--------CNKISGICACKTAFFGSKCDRCNEGYYGFPN---CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGT 3781
T G C+ C+ Y G C +C+CNN+ +C DP G C C +NT G C +C+ F+ G +L+ C C C G + +C + + G+C+C+ RQCD+C G Y LK C+ C C G ++ C+ + GQC+C NV G C+ C G++ N P+ C+ C C P + CD +G+C+C + C CE+ ++ + + C CNC P CD G C C V + C +C GY+ L GC C P G + G C C C +C + ++ + + GC+ CNC G+ C+ +G CAC T G +CD+C YY P+ C PC+C +G+ + T C+ TG CQCK N+ G +C C G++ G+
Sbjct: 531 TQGEKCESCLPMYNNKAFLRGTNDDPYPCVKCECNNHATACYYNSSLDPSPASRTVGGGGVCINCLHNTAGQFCHVCRDDFFREPGKSLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAGTVNRNASCDQSRGQCYCKSNVTGRTCDTCESGFYGLRDNDPSGCRACECDPFGTLPGSVCDAGSGQCRCKAGSTGLRCHLCEDGYHSLDSSGCKTCNCSTIGAKPGLVNTCDKTNGQCYCKTYVEGKTCDRCKTGYYNLTTVNQDGCSLCSCDPKGIQGSSLTCEVSSGNCTCKKYVREPTCGQCMKGYFNLSSSNPEGCQACNCNPKGTLGGDRTPAGELECSGSNGKCACLTNVQGLRCDQCVSAYYWNPSGYGCSPCNCDASGSLA---TNCNS-TGYCQCKPNIGGRRCDRCMPGSYGGPGS 996 HSP 4 Score: 130.568 bits (327), Expect = 9.756e-30 Identity = 96/295 (32.54%), Postives = 133/295 (45.08%), Query Frame = 2
Query: 2246 KCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHS------NTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYY----GNPKKKISCKRCKCN-----------NNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN 3067
+C C G GL C CE GY +++ C+ C C NTCD NG+C C G C+ C +GYY + + N + C C C Q + L SS N C K + + C +C++GY+ NP+ C+ C CN + C G+C C N G C C +Y + C C+CD+ GS + NC+ TG C C+ N R+CD C G Y C PCNC + G +S C+ SG+C C NV GD+C+ C +G N
Sbjct: 760 QCRCKAGSTGLRCHLCEDGYHSLDS----SGCKTCNCSTIGAKPGLVNTCDKTNGQCY-CKTYVEGKTCDRCKTGYY-NLTTVNQDGCSLCSCDPKGIQGSSLTCEVSSGNCTCKKYVREPT---CGQCMKGYFNLSSSNPE---GCQACNCNPKGTLGGDRTPAGELECSGSNGKCACLTNVQGLRCDQCVSAYYWNPSGYGCSPCNCDASGSLATNCNS-TGYCQCKPNIGGRRCDRCMPGSYGGPGSCKPCNCNMAGSLSDT--CD-DSGKCNCKPNVQGDKCDTCKDGTQN 1038 HSP 5 Score: 127.487 bits (319), Expect = 8.285e-29 Identity = 125/462 (27.06%), Postives = 192/462 (41.56%), Query Frame = 2
Query: 329 RNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDL-KQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHN----TCGSNCETCCPLFNQRPW------KPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKP----GDCICKKGYEGVNCDLCSIGYERYPNGE---CLPCPCSIDGSV--RTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
R T HP E+ D +WQS N V +DL Q QI ++++ + P P ++ERS D G Y PW +FA S + + + + D + C Y Q + ++ I G S + + P T A +R + H + + H+ Y++L+ + + G+C C+ HA K + N S +C C+ + T G CE+C P++N + + P C +C CN + C YN ++ S + GGGVCINC NT G C C F+R + + C+PCQC G G S D D P G C CK+ +G CD C G+ + C+ C C+ G+V S + + C CK +V + C TC++G++ L +P GC C C
Sbjct: 326 RLATTAHPSEYMTDADTTTYWQSAVT------DNATVEVDLLYQELQIFFVIM-LFYGPPPEAILIERSRDRGSTYKPWQYFASDCQSSFGMENNG------------NLVNPDSINCIQY--QRCECHGHAQKVTFIKGNSS--SPGRCRCECDPSTFTQGEKASNVRIQLYNHTLIQNE--KHR-----------YFALKRVLVTGRCECHGHAQKVTFIKGNSSSPGRCRCECDPSTFTQGEKCESCLPMYNNKAFLRGTNDDPYP-CVKCECNNHATACYYNSSLDPSPASRT----VGGGGVCINCLHNTAGQFCHVCRDDFFREPGKSLSAVDVCSPCQCTGPGVETGKTSCARD--DSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAGTVNRNASCDQSRGQCYCKSNV-TGRTCDTCESGFYGLRDNDPSGCRACEC 743 HSP 6 Score: 90.8929 bits (224), Expect = 9.017e-18 Identity = 106/400 (26.50%), Postives = 148/400 (37.00%), Query Frame = 2
Query: 2798 KCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKC-IPCNCEIQGRISSEKFC---NTTSGQCFC----LENVNGDRCEKCVEGYFN-----------YPNCKKCLCSPHALS-----------ADCDLNNGKC--KCGSNVINRICDKCENLHYWNNRTD------CVPCNC-GRGSLNPKCD---------METGNCKCAQNVARRDCSKCDVGYFGLDV---TGCKKCPPCYNGQVCNN------EGICVCPPNTIGNLCNKCSEDSWDF--NETIGCKKCNCYINGSY-SNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 3811
+C+CD ST +K + + R N Y K+ + CE G F +++ G+C C G++CE C+ Y N YP C KC C+ HA + A + G C N + C C + + C PC C G G K + G C+C Q R C C G++ L TGC C G V N G C C N G C+ C + N+ GC+ C C G+ ++C+ SG C CK G +C C +GY+ C+ C+C+ G K + CDK GQC CK V G C C+ G + + GC C C
Sbjct: 453 RCECDP--STFTQGEKAS---NVRIQLYNHTLIQNEKHRYFALKRVLVTGRCECHGHAQKVTFIKGNSSSPGRCRCECDPSTFTQGEKCESCLPMYNNKAFLRGTNDDPYP-CVKCECNNHATACYYNSSLDPSPASRTVGGGGVCINCLHNTAGQFCHVCRDDFFREPGKSLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAGTVNRNASCDQSRGQCYCKSNVTGRTCDTCESGFYGLRDNDPSGCRACECDPFGTLPGSVCDAGSGQCRCKAGSTGLRCHLCEDGYHSLDSSGCKTCNCSTIGAKPGLVNTCDKTNGQCYCKTYVEGKTCDRCKTGYYNLTTVNQDGCSLCSC 846 HSP 7 Score: 78.5666 bits (192), Expect = 4.873e-14 Identity = 59/174 (33.91%), Postives = 73/174 (41.95%), Query Frame = 2
Query: 4538 KEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKV-----------CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQC 5026
K YFNL +S G + CNCN + C G C C+ N +G +C C +Y N Y C PC C A + A NC CQC+ G+ C+ C G YG P CK C+CN GS S C++ G C C P V G KCD C
Sbjct: 881 KGYFNLSSSNPEG-----------CQACNCNPKGTLGGDRTPAGELECSGSNGKCA-CLTNVQGLRCDQCVSAYYWNPSGYG-CSPCNC-DASGSLATNCNSTG-----YCQCKPNIGGRRCDRCMPGSYGGPGS---CKPCNCNMAGSLSDTCDDSGKCNCKPNVQGDKCDTC 1032 HSP 8 Score: 75.485 bits (184), Expect = 5.228e-13 Identity = 70/233 (30.04%), Postives = 90/233 (38.63%), Query Frame = 2
Query: 998 GISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGR-MEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPP---CNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLS 1684
G+ +C CK T G C+ C P F C C CN +VN+ + G C C+ N G C+ C GFY + + C C+C GT+ V D G C CK G G+ C LC GY + C C CS G+ C C CK +V C CK GY+NL + N +GC C C Q SSL+
Sbjct: 650 GVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAG--------------TVNRNASCDQSRGQCY-CKSNVTGRTCDTCESGFY---GLRDNDPSGCRACECDPFGTLPGSVC------DAGSGQCRCKAGSTGLRCHLCEDGYHSLDSSGCKTCNCSTIGAKPGLVNTCDKTNGQCYCKTYVEGKT-CDRCKTGYYNLTTVNQDGCSLCSCDPKGIQGSSLT 857 HSP 9 Score: 66.6254 bits (161), Expect = 2.012e-10 Identity = 79/254 (31.10%), Postives = 102/254 (40.16%), Query Frame = 2
Query: 914 YYSLRNIEIGGKCICNNHADKCDKVIENG------ISVAKCNCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRME---GGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGS-CVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPP--CNCKVHVNSDDLCKTCKNGYFNLDSANPEGC 1636
YY+L + G +C+ CD G +S C CK C C FN P C+ CNCN K TL G +E G C C N +G+ C++C+ +Y WN Y C+PC C G++ + C S G C CK G CD C G P G C PC C++ GS+ + C CNCK +V D C TCK+G NL N GC
Sbjct: 830 YYNLTTVNQDGCSLCS-----CDPKGIQGSSLTCEVSSGNCTCKKYVREPTCGQCMKGYFNLSSSNPEG-CQACNCNPK----------GTLGGDRTPAGELECSGSNGKCA-CLTNVQGLRCDQCVSAYY--WNPSG---YGCSPCNCDASGSLATNCNSTGY---------CQCKPNIGGRRCDRCMPGSYGGP-GSCKPCNCNMAGSLSDT---CDDSGKCNCKPNVQGDK-CDTCKDGTQNLQVNNDFGC 1047 HSP 10 Score: 65.855 bits (159), Expect = 3.589e-10 Identity = 46/150 (30.67%), Postives = 67/150 (44.67%), Query Frame = 2
Query: 4631 GYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYK-NDKNYDQCEPCQC-PSAHYNFAQN-CTRIE-SSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSC 5068
G S C+ +G+C C C C +G++ + N + C+ C C P + +E S KC C G C+ C +Y NP G C C+C+ GS + NCN G CQC P + G +CD+C P +C+ C
Sbjct: 853 GSSLTCEVSSGNC-TCKKYVREPTCGQCMKGYFNLSSSNPEGCQACNCNPKGTLGGDRTPAGELECSGSNGKCACLTNVQGLRCDQCVSAYYWNP-SGYGCSPCNCDASGSLATNCNSTGYCQCKPNIGGRRCDRCMPGSYGGPGSCKPC 1000 HSP 11 Score: 60.4622 bits (145), Expect = 1.484e-8 Identity = 42/134 (31.34%), Postives = 62/134 (46.27%), Query Frame = 2
Query: 4661 GDCQNCMGNREGNKCQLCREGFY----KNDKNYDQCEPCQCPSAHYNFAQ-NCTRIESS---LELKCQCRIGYSGKSCENCQQGFYG-NPIKGIPCKSCDCNQQGSQSLNCN---EFGICQCSPGVLGIKCDQC 5026
G C NC+ N G C +CR+ F+ K+ D C PCQC + +C R +S+ + +C+C+ G+ C+ C+ GFY C +C CN G+ + N + G C C V G CD C
Sbjct: 590 GVCINCLHNTAGQFCHVCRDDFFREPGKSLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAGTVNRNASCDQSRGQCYCKSNVTGRTCDTC 723 HSP 12 Score: 51.6026 bits (122), Expect = 8.609e-6 Identity = 42/163 (25.77%), Postives = 75/163 (46.01%), Query Frame = 2
Query: 4604 INLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQ-CEPCQCPSAH-YNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYG---NPIKGIPCKSCDCNQQGSQSLNCNEFGI--CQCSPGVLGIKCDQCAPNY-VVEKKTCQSC 5068
+ K +C + G C+ C +G +C C+ GFY ++ C C C +A N +C + +C C+ +G++C+ C+ GFYG N G C++C+C+ G+ + + G C+C G G++C C Y ++ C++C
Sbjct: 635 VETGKTSCARDDSTPGVIAGQCE-CKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAGTVNRNASCDQSRG----QCYCKSNVTGRTCDTCESGFYGLRDNDPSG--CRACECDPFGTLPGSVCDAGSGQCRCKAGSTGLRCHLCEDGYHSLDSSGCKTC 790
BLAST of Laminin subunit alpha-2 vs. Ensembl Medaka
Match: lamc1 (laminin subunit gamma 1 [Source:NCBI gene;Acc:100529197]) HSP 1 Score: 428.328 bits (1100), Expect = 3.834e-122 Identity = 313/1056 (29.64%), Postives = 492/1056 (46.59%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFA---KSDDDNLWWQSPSMAQGLKYQ-NVNVTIDLKQVYQIVYILLKMGDSPRPGNW-ILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEM--FCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEG-GGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSL---SAKNLKISDLKG---WKLTTFTGEKIALPKFTSFQYLT-SENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFR-----LFHSVSIEDRRLQRQVFIYIKPTE--WKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLN----ITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQ-GQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSY-SSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKK----ISCKRCKCNNNQE-----SCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALT----KSCKKCDCDSLGSTSE--NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWN 3205
N + ++ TCG+ PE +C + + C +CD R+ H + + D WWQS +M G++Y ++N+T+ L + + I Y+ LK S RP ++ I +R+ +G ++P+ +++ SC +Q+ N ADDE CT +S L G + S + GR S N + + L ++++A I+ + T ++ + + + YYY++ + +GG+C CN HA +C ++N + C+CKHNT G +C TC P FN RPW+ C CNCN KS EC ++ ++ R G GG C NC +NT+G NCE+C+ +YR + C PC C +G++ G G C CK G G CD C GY C C CS GS + C CK +V C CK G+FNLD NP+GC PC+CF ++ C+S S ++ S G W+ G +++ S Q ++ S +S++ Y+ AP +LGN I SYG ++E + + + D+I++ + R + + + +Q VF T+ W+ + +F L + + I+ Y +++S L+ +T RG A +E+C+CP G+ G CE C GYRR L CE C+C HS+ C+ + G C +C NTAG CE C G+YGDA + N C+ CPCP C+ S+ VC C T+G C+ C +G++G+P + SC +C C+ N + +C+ TGEC +C +T G C CK GFYG+AL+ K CK C C G+ + +C ++TG+C+C N R C +C G + K C CNC G + + C+ +GQC C V+G C++C +F + + CK C C S+ ++G+C+C + CD CE +++N
Sbjct: 46 NVTVVATNTCGSP-PEEYCVQTGATGVTKS--------CHICDARDPRNHHGAAYLTDYNNHQDATWWQSQTMLAGVQYPYSINLTLHLGKAFDITYVRLKFHTS-RPESFAIYKRTKEGGPWVPYQYYS---GSCEKTYQK--------VNRGFIRTADDEQQALCTDEFSDISPLTGGNVAFSTLEGRPSSHNFDNSPV------LQDWVTATDIKVTLNRLNTFGDEV-----FNDPKVLKSYYYAISDFAVGGRCKCNGHASEC---VKNSVGRLVCDCKHNTAGDDCNTCRPFFNDRPWRRATADSANECLSCNCNGKSAECYFDP----------ELYRATGHGGHCRNCADNTDGPNCERCLDNYYRDQSG-----NRCQPCSCSPIGSLSPQCDRG--------GVCACKPGVTGEKCDRCLPGYHSLTEAGCRQCSCSPAGSTQECDV-ATGQCRCKENVEGFS-CDRCKLGFFNLDPHNPQGCTPCFCFQHSSVCNSAEGYSVHSITSSFTNGNEQWRGQQQDGSSVSIQWSPSGQEVSLSSDSFL--------PMYFVAPEKFLGNQILSYGQNLSLNFRVE----RRDHRLSAEDLILEGANMRVAVPLIAQGNAYPNENMQTYVFRLHDSTDYPWR-------PHISHADFQKLLQNLTAIKIRGTY--SERSAGFLDDVSLVTARRGPGVPAR----WVEQCDCPQGYQGQHCEQCTLGYRRARPDLGAFSPCEPCSCNGHSDACNPVTGAC-DCQDNTAGLSCERCKDGFYGDATQGSPNDCQPCPCP-----GGATCAIVPSTREVVCTNCPTGTTGKRCELCDDGHFGDPLGQNGPVRSCHKCMCSGNIDLNAVGNCNRETGECLKCVNDTEGFFCDRCKEGFYGNALSTGPGKRCKSCSCSPFGTVGQQTSCSEVTGQCECLPNVAERDCSACQPGHFNLQSGKGCERCNCNPIGSTNGQ--CDIVTGQCECQPGVSGLHCDRCEVNFFGFSSSGCKPCDCDSEGSSSGQCKDDGRCQCRPGFVGARCDMCEENYFYN 1008 HSP 2 Score: 164.466 bits (415), Expect = 9.440e-40 Identity = 156/577 (27.04%), Postives = 252/577 (43.67%), Query Frame = 2
Query: 3419 CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGF--PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLN-ANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQ---YQNDILDKFII-LEDSN--VINSNLTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN--DKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPI-KGIPCKSC-DCNQQGSQSL----NCN-EFGIC-QCSPGVLGIKCDQCAPNY------VVEKKTCQSC 5068
C NT G C +C ++ + C+ C+C GS S C++ G+CACK G KCDRC GY+ C+ CSC+ G+ +CD TGQC+CK+NV G C C+ G F D GC CFCF S C++ + + +SI + +D S++ + + + F P+Y + F + + + +++ +N V+ D++ E ++ +E N MR V +I+Q Y N+ + ++ L DS +++ F L N+T++K++ Y+++ L + ++T + + G VE+C C G HC+ GY + +L + C+CNG+S C+ VTG C +C N G C+ C++GFY + + + C+PC CP C + S+ E+ C C G +GK CE C G +G+P+ + P +SC C G+ L NCN E G C +C G CD+C + K C+SC
Sbjct: 356 CADNTDGPNCERCLDNYYRDQSGNRCQPCSCSPIGSLSPQCDR-GGVCACKPGVTGEKCDRCLPGYHSLTEAGCRQCSCSPAGSTQ----ECDVATGQCRCKENVEGFSCDRCKLGFFNLDPHNPQGCTPCFCFQHSSVCNSAEGYSV--HSITSSFTNGNEQWRGQQQDGSSVSIQWSPSGQEVSLSSDSFLPMYFVAPEKFLGNQILSYGQNLSLNFRVERRDHRLSAE-----DLILEGAN----MRVAVP----------------------LIAQGNAYPNENMQTYVFRLHDSTDYPWRPHISHADFQKLLQNLTAIKIRGTYSERSAGFLDDVSLVTAR-----RGPGVPARWVEQCDCPQGYQGQHCEQCTLGYRRARPDLG-------------AFSPCEPCSCNGHSDACNPVTGAC-DCQDNTAGLSCERCKDGFYGDATQGSPNDCQPCPCPG-----GATCAIVPSTREVVCTNCPTGTTGKRCELCDDGHFGDPLGQNGPVRSCHKCMCSGNIDLNAVGNCNRETGECLKCVNDTEGFFCDRCKEGFYGNALSTGPGKRCKSC 870 HSP 3 Score: 152.91 bits (385), Expect = 3.076e-36 Identity = 109/345 (31.59%), Postives = 160/345 (46.38%), Query Frame = 2
Query: 2576 CLHNTSGVHCDRCIEGYY-GNPKKKI--SCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP-------NCKKCLCSPHAL---SADCDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC----PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC-NCY 3514
C G HC++C GY P C+ C CN + ++C+PVTG C C NT G C+ CK GFYGDA S C+ C C + + C +C T ++C+ C G + + C C C +++ CN +G+C C+ + G C++C EG++ CK C CSP C G+C+C NV R C C+ H+ + C CNC GS N +CD+ TG C+C V+ C +C+V +FG +GCK C +GQ C ++G C C P +G C+ C E+ + GC++C +CY
Sbjct: 678 CPQGYQGQHCEQCTLGYRRARPDLGAFSPCEPCSCNGHSDACNPVTGACDCQDNTAGLSCERCKDGFYGDATQGSPNDCQPCPCPGGATCAIVPSTREVVCTNCPTGTTGKRCELCDDGHFGDPLGQNGPVRSCHKCMCSGNIDLNAVGNCNRETGECLKCVNDTEGFFCDRCKEGFYGNALSTGPGKRCKSCSCSPFGTVGQQTSCSEVTGQCECLPNVAERDCSACQPGHFNLQSGKGCERCNCNPIGSTNGQCDIVTGQCECQPGVSGLHCDRCEVNFFGFSSSGCKPCDCDSEGSSSGQ-CKDDGRCQCRPGFVGARCDMCEENYFYNRSVPGCQQCPSCY 1021 HSP 4 Score: 117.857 bits (294), Expect = 1.453e-25 Identity = 97/342 (28.36%), Postives = 147/342 (42.98%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKDKK-------CIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFN-----YPN-CKKCLCSPHALSADCDLNNGKC--KCGSNVINRICDKCENLHYWNNR------TDCVPCNC-GRGSLNP--KCDMETGNC-KCAQNVARRDCSKCDVGYFG--LDVTGCKKCPPC-------YNGQVCNNE--GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTF 3766
+CDC + + C+ CT G Y + + C PC+C CN +G C C +N G CE+C +G++ PN C+ C C A A C + + C+ C++ H+ + C C C G LN C+ ETG C KC + C +C G++G L K+C C Q +E G C C PN C+ C ++ GC++CNC GS + C+ ++G C C+ G CDRC ++GF + C+PC C G+ S + K G+CQC+ +G +C +C + F
Sbjct: 675 QCDCPQGYQGQHCEQCTLG-YRRARPDLGAFSPCEPCSCN-----GHSDACNPVTGACDCQDNTAGLSCERCKDGFYGDATQGSPNDCQPCPCPGGATCAIVPSTREVVCTNCPTGTTGKRCELCDDGHFGDPLGQNGPVRSCHKCMCSGNIDLNAVGNCNRETGECLKCVNDTEGFFCDRCKEGFYGNALSTGPGKRCKSCSCSPFGTVGQQTSCSEVTGQCECLPNVAERDCSACQPGHFNLQSGKGCERCNCNPIGSTNGQCDIVTGQCECQPGVSGLHCDRCEVNFFGFSSSGCKPCDCDSEGSSSGQC----KDDGRCQCRPGFVGARCDMCEENYF 1006 HSP 5 Score: 101.679 bits (252), Expect = 1.374e-20 Identity = 165/714 (23.11%), Postives = 285/714 (39.92%), Query Frame = 2
Query: 4613 KKCNCNGYSKV-----CDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLD-LRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIE----KLKTRYNKVLEDLRSTFTDIN-ETISGHEQLGISNELIKNATAYLETIKNIYK-----------EMPNITLAASVLKDVL-KYVDELNQIIKYTQ-SHIQSDQINN--LKILKNKTIMADEINTNNKEYLNTMSTKK---KEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNH------LVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYS---NITRDILAVDYAINQTKKELD-----FNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRN-----LEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSEL 6595
K C+C+ + V C VTG C+ C+ N C C+ G + N ++ CE C C Q C + +C+C+ G SG C+ C+ F+G G CK CDC+ +GS S C + G CQC PG +G +CD C NY + + C SCY LVR D +N+ K L + +D L + +S + + L K I + Q KD + ++ L T++N+ L+ +R+T D + ++ + LI A L+ K+ E N+TL A + + K+ E +QI K + ++ S + N LK L+ ++ EI NK+Y + K K+ + +E KIF +T + DA K+ +DE + I ++++L+ + N+ L SK + L+K +Q L + A++ K ++ NI D+ D +N K + N I S + E + EN ++ E+ K N K K EK + DV + LS + + + + + + + N K D ++K++KV+ + L N +K+ D+ +S+K +E+++ +++ +L
Sbjct: 868 KSCSCSPFGTVGQQTSCSEVTGQCE-CLPNVAERDCSACQPGHF-NLQSGKGCERCNCNPIGSTNGQ-CDIVTG----QCECQPGVSGLHCDRCEVNFFGFSSSG--CKPCDCDSEGSSSGQCKDDGRCQCRPGFVGARCDMCEENYFYNRSVPGCQQCPSCYG------LVR-DKVNQQ--RQKLHDLQTLIDNLGSGQETVSDKAFEDRLKEAEK------AIAELLSEAQASKDLDRGLMDRLDTINNTLTTQWNR-LQSIRNTVDDTGAQADRARSRVQDAESLIDRARQELDKAKDALSKVDIKPPTGTGEPNNMTLLAEEARRLAEKHKTEADQIEKIAKDANDTSTKAYNLLLKTLEGESRTGQEIEELNKKYNESKELAKNLEKQAKKVQDEADEAGNQALKIFANLT--------SLPPFDA--KALEDEANKIKKEATDLDNLVDKMEKNYNDMRDDLKSKEQEVRKLLEKGKSEQQTADQLLARADAAKALAEEAAQKGKATFAEAENILEDLRDFDRRVNDNKTAAEEALKKIPAINATIMSANEKTREAKTALGNATENALEAKRKAEEAEKIASNVQIGSAKTKEDAEKALQDTNNLENDVNNMMDQLSAAEQDLAKKKVEAD--ADMMMAAMASDNAKEAEDNARKAKNMVKKVLKVITDLLAQLGNIDKVDLSKLNDIDESLKKAKEKMRDSKLDEKL 1544 HSP 6 Score: 87.0409 bits (214), Expect = 3.597e-16 Identity = 76/261 (29.12%), Postives = 116/261 (44.44%), Query Frame = 2
Query: 2726 NTMGTEC----QLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGK--CDCRTNFVNRQCDSCTSGLYLKDK-----------KCIPCNCEIQGR---ISSEKFCNTT-SGQCF-CLENVNGDRCEKCVEGYFNYPN---CKKCLCSP-HALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY---NGQVCNN-EGICV 3418
NT G E ++ K +Y + +C C+ G SE G+ CDC+ N C++C + D+ +C+ CNC + E + T G C C +N +G CE+C++ Y+ + C+ C CSP +LS CD G C C V CD+C ++ C C+C +CD+ TG C+C +NV C +C +G+F LD + C PC+ + VCN+ EG V
Sbjct: 243 NTFGDEVFNDPKVLKSYYYAISDFAVGGRCKCN--GHASECVKNSVGRLVCDCKHNTAGDDCNTCRP--FFNDRPWRRATADSANECLSCNCNGKSAECYFDPELYRATGHGGHCRNCADNTDGPNCERCLDNYYRDQSGNRCQPCSCSPIGSLSPQCD-RGGVCACKPGVTGEKCDRCLPGYHSLTEAGCRQCSCSPAGSTQECDVATGQCRCKENVEGFSCDRCKLGFFNLDPHNPQGCTPCFCFQHSSVCNSAEGYSV 498 HSP 7 Score: 66.6254 bits (161), Expect = 5.584e-10 Identity = 69/267 (25.84%), Postives = 114/267 (42.70%), Query Frame = 2
Query: 4295 NVINSNLTRDIFMMALLNVTSLKVKLYTDKV-NPPLIKIKIITYKVMETTQAIGKRVT---AVEKCICNAL----------TFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQC 5026
N NS + +D + VT ++ + D+V N P + +K Y + + A+G R +C+ N++ T G+ C + +FN + + + N L CNCNG S C G C+NC N +G C+ C + +Y+ D++ ++C+PC C S + + C R C C+ G +G+ C+ C G++ G C+ C C+ GS G C+C V G CD+C
Sbjct: 219 NFDNSPVLQDWVTATDIKVTLNRLNTFGDEVFNDPKV-LKSYYYAISDF--AVGGRCKCNGHASECVKNSVGRLVCDCKHNTAGDDCNT-----CRPFFN-DRPWRRATADSANECL----SCNCNGKSAECYFDPELYRATGHGGHCRNCADNTDGPNCERCLDNYYR-DQSGNRCQPCSC-SPIGSLSPQCDRGGV-----CACKPGVTGEKCDRCLPGYHSLTEAG--CRQCSCSPAGSTQECDVATGQCRCKENVEGFSCDRC 463 HSP 8 Score: 64.6994 bits (156), Expect = 1.922e-9 Identity = 59/197 (29.95%), Postives = 85/197 (43.15%), Query Frame = 2
Query: 4517 NPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC--DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNY--------DQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY-VVEKKTCQSC 5068
N ++ + E FN P KS + + +C CNG++ C +SV +C N G+ C CR F ND+ + ++C C C SA F R C G +CE C +Y + G C+ C C+ GS S C+ G+C C PGV G KCD+C P Y + + C+ C
Sbjct: 240 NRLNTFGDEVFNDPKVLKSYYYAISDFAVGG--RCKCNGHASECVKNSVGRLVCDCKHNTAGDDCNTCRPFF--NDRPWRRATADSANECLSCNCNGKSAECYFDPELYRATGHGGHCRNCADNTDGPNCERCLDNYYRDQ-SGNRCQPCSCSPIGSLSPQCDRGGVCACKPGVTGEKCDRCLPGYHSLTEAGCRQC 431
BLAST of Laminin subunit alpha-2 vs. Ensembl Medaka
Match: lamc3 (laminin subunit gamma 3 [Source:NCBI gene;Acc:105355198]) HSP 1 Score: 368.622 bits (945), Expect = 5.541e-103 Identity = 308/1068 (28.84%), Postives = 466/1068 (43.63%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFA---KSDDDNLWWQSPSMAQGLKY-QNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYL---------EQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVA-----VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKP---PCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSS------LSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQY---LTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKI-----GKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH--IEKCNCPPGFNGLSCESCESGY-RRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLD--YQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS----CKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS----CKKCDCDSLGSTSE--NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTD 3217
N + +S CG+ PE +C + S C CD + H +++ WWQS SM G++Y ++VN+T+ L + ++I YI LK S I +R+ +G ++P+ +++ SC + + + ++L CT +S L G + S + GR S N + Q+L FL+ A DL+ L + + Y+Y++ + +GG+C CN HA +C +E C C+H+T G++C+ C P + RPW C +CNCN +S++C ++ + + GG C++C++NT+G +CE C YR +A E PC C C+ G++ S N E G C+C+ G G C++C G+ C PC C GSV C P C CK +V C CK G+FNL NP GC+PC+CFG + CSS ++ + I D W F GE+ L Y LT E+S ++ AP +LG+ I SYG E L SL VI++ L L + ++ P+ I P +A + + + L + L I N S +L +TL+ + +P +E+C+CP GF+G CE C G+ R+ C C C+ H CD G C+ C+H T G CE C GYYG+A C+ CPCP Q+ E + VC C +G+ C C +GYYG+P + C RC CN N + CD TG+C +C +T G CQ C+ GFYG+AL ++ CK C C S G++ C TG C C ++ R C C G + C C C G S+ + C+ +G+C C V G C+ C G+F + + C+ C C P ++S C NG C+C + CDKCE ++Y++NR +
Sbjct: 53 NRTVEASNVCGST-PEDYC-----------MQTGSTRSCHYCDASDPELSHNASLLTDFHRNEEPTWWQSQSMFYGIQYPKSVNLTLHLGKAFEITYIRLKFYTSRPESFAIYKRTEEGGPWLPYQYYSA---SCKKTYGKDAKGFIRPGEEEKTAL------CTDEFSDISPLTGGNVAFSTLEGRPSAYNFD--------QSL------------FLQEWVTATDLLISLDRLNTFGDEFFKDAKVLRSYFYAISDFSVGGRCKCNGHASEC---VEGERGDLVCACQHHTVGADCQKCHPFYQDRPWARATGDSANECLKCNCNGRSDQCIFD---------MEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYR----VAPE-DPCLACNCNINGSV-SLQCNAE-------GRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGSVG----ECSPLDGQCFCKRNVEGHR-CGRCKPGFFNLQLQNPAGCQPCFCFGHSLACSSSNHYTPVNITSDFIEDQDRWVGEFFGGEEYILQWKEGEVYLLPLTEEDS-----------GFYKAPDKFLGHQIYSYGQPLHITFTSETYDLLPNSLT----VILEGSGMTL-------SADLYPKPILHPDPSNSPILHEYETSFKPRLSAFELQHL--LYNLTALKISNAGGHNYTS-QLARVTLASASISSRPLSLPAAWVEQCSCPLGFSGQFCEGCAPGFTRQEPGGGPLSACVPCKCHQH-GICDPETGVCE-CSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPDRSSCVQIAE------TGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIG--STSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVSMQCH-GNGTCQCRQGFVGYKCDKCE-VNYFHNRAN 1012 HSP 2 Score: 126.716 bits (317), Expect = 2.995e-28 Identity = 140/571 (24.52%), Postives = 222/571 (38.88%), Query Frame = 2
Query: 3407 GICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY--GFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEK---NSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQ-NDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKND--KNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIKGI----PCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSG 5077
G CV C NT G C C + + C CNC INGS S CN G C C+ G+KC+ C +G++ G C+PC C +G+ + +C + GQC CK+NV G +C C+ G F GC CFCFG S C + NH N ++ I D+ G++Y L + PLT + F H + + + + + + + D +P V +E + + +E+ + K +S ++ +L L+ SN N T + + L + + +++ + VE+C C G C+ G+ ++ G L C C+ + +CD TG C+ C G C+ C G+Y N C+PC CP +C +I + ++ C C G +G C+ C+ G+YG+P+ PC C+CN +++ N GIC G +C +C + E CQ C G
Sbjct: 355 GRCVDCRDNTDGAHCEHCKANHYRVAPEDPCLACNCNINGSVSLQCNA-EGRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGS----VGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCFCFGHSLACSSS-NHYTPVNITSDF-IEDQDRWVGEFFGGEEYI---LQWKEGEVYLLPLTEEDSGFYKAPDKFLGHQIYSYGQPLHITFTSETYD-----LLPNSLTVILEGSGMTLSADLYPKPILHPDPSNSPILHEYETSFKPRLSAFELQHLLYNLTALKISNAGGHNYTSQLARVTLASASI--------------------------SSRPLSLPAAWVEQCSCPLGFSGQFCEGCAPGFTRQ-------------EPGGGPLSACVPCKCHQHG-ICDPETGVCE-CSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPD-----RSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCN----GNVDFNAVGICDHFTG----QCLKCMGH--TEGDHCQRCQRG 854 HSP 3 Score: 120.553 bits (301), Expect = 2.453e-26 Identity = 99/338 (29.29%), Postives = 143/338 (42.31%), Query Frame = 2
Query: 2591 SGVHCDRCIEGYY-----GNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP-------NCKKCLCSPHALSA---DCDLNNGKCKCGSNVINRICDKCENLHYWNNRT--DCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC 3505
SG C+ C G+ G P +C CKC+ + CDP TG C CS+ T G C+ C G+YG+AL + C+ C C S + + C +C +C C G Y + C+ CNC ++ C+ +GQC C+ + GD C++C G++ CK C CS S +C G C C S+V R C CE + ++N + C C C GS + C TG C C V C C +G+FG GC+ C P C+ G C C +G C+KC + + C++C
Sbjct: 685 SGQFCEGCAPGFTRQEPGGGPLS--ACVPCKCHQHG-ICDPETGVCECSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPDRSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCE-VGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVSMQCHGNGTCQCRQGFVGYKCDKCEVNYFHNRANHQCEEC 1018 HSP 4 Score: 117.087 bits (292), Expect = 2.869e-25 Identity = 111/405 (27.41%), Postives = 167/405 (41.23%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSADCDLNNGKC---KCGSNVINRICDKCENLHYWNNRTD------CVPCNC-GRGSLNPK--CDMETGNC-KCAQNVARRDCSKCDVGYFG--LDVTGCKKCPPC------YNGQV--CNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC-FCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANY 3952
+C C F + C+ C G ++ C+PC C G C+ +G C C G CE C+ GY+ +C+ C C P S G+ C + C CE+ +Y + CV CNC G N CD TG C KC + C +C G++G L+ T +KC PC +G V C+ + G C C + G C+ C ++ GC++C C GS S C+ +G C C+ G+ CD C G++GF + C+ C+C G+ S +C G CQC+Q +G KC C F + ++ C C C+ + +K L R+ + H FD + L + H+ Y
Sbjct: 677 QCSCPLGFSGQFCEGCAPGFTRQEPGGGPLSACVPCKCHQHG------ICDPETGVCECSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPC-PDRSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVS---MQCHG-NGTCQCRQGFVGYKCDKCEVNYFHNRANHQ--CEECPVCYSLVKKQAEKLQTRLQDLEKLLSH-FDCRGRLGRQPNLQQYHMAGFY 1067 HSP 5 Score: 102.449 bits (254), Expect = 6.553e-21 Identity = 95/354 (26.84%), Postives = 141/354 (39.83%), Query Frame = 2
Query: 4484 CNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNG-----YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN---DKNYDQCEPCQCPSA----HYNFAQ------------------------------------NCTRIESSLEL------KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYSGCTKD---LLVRIDTLNETVINF----KWKTLPSTLDLRLNKINISIQMSSE--LLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYN 5341
C A G CQ GYY + +SG++R R CNCNG +CD TG C CMG+ EG+ CQ C+ GFY N + +C+PC C SA H N C+ I S+ + +C CR G G C++C+ GF+G +G C++C+C+ GS S+ C+ G CQC G +G KCD+C NY + + C CYS K L R+ L + + +F + P+ + Q+ E L N++ F + + F QF ++ ++ L N
Sbjct: 782 CPAGQTGMRCQMCEDGYYGDPMG-----QSGTVRPCVR-------CNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRG--CRACNCDPMGSVSMQCHGNGTCQCRQGFVGYKCDKCEVNYFHNRANHQCEECPVCYSLVKKQAEKLQTRLQDLEKLLSHFDCRGRLGRQPNLQQYHMAGFYQHDQLRGEDSLPNALEDFLAFQEAREAFIKQFSSLEAATGTLSAQLHGLTFTLN 1121 HSP 6 Score: 79.337 bits (194), Expect = 7.406e-14 Identity = 70/227 (30.84%), Postives = 97/227 (42.73%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-----CRCSYNTMGTECQLCKHGFYGDA--------LTKSCKKCDCDSLGSTSENC---------DKLTGKC-DCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--PNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY---WNNRTDCVPCNCGRGSL 3250
RCKCN + C V GE C C ++T+G +CQ C H FY D C KC+C+ S+ C G+C DCR N C+ C + Y + C+ CNC I G +S + CN G+C C V G +C C +G+ + C+ C C +C +G+C C NV C +C+ + N C PC C SL
Sbjct: 275 RCKCNGHASEC--VEGERGDLVCACQHHTVGADCQKC-HPFYQDRPWARATGDSANECLKCNCN---GRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPEDPCLACNCNINGSVSLQ--CN-AEGRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGSVGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCFCFGHSL 492 HSP 7 Score: 61.6178 bits (148), Expect = 1.675e-8 Identity = 60/213 (28.17%), Postives = 84/213 (39.44%), Query Frame = 2
Query: 4478 CICNALTFGNHCQ--NPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY----VVEKKTCQSCY 5071
C C T G CQ +P Y + P + +G + N L KCNCNG S C G C +C N +G C+ C+ Y+ D C C C ++ + E +C CR G +G C CQ GF+ G C+ C+C+ GS G C C V G +C +C P + + CQ C+
Sbjct: 295 CACQHHTVGADCQKCHPF------YQDRPWARATGD--SANECL----KCNCNGRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPE-DPCLACNCN------INGSVSLQCNAEGRCVCRPGVTGAKCNMCQDGFHSLGPSG--CRPCECHHSGSVGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCF 486 HSP 8 Score: 55.8398 bits (133), Expect = 1.057e-6 Identity = 49/152 (32.24%), Postives = 66/152 (43.42%), Query Frame = 2
Query: 4616 KCNCNGYSKVC-DSVTGDCQ-NCMGNREGNKCQLCREGFYKN-------DKNYDQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+C CNG++ C + GD C + G CQ C FY++ + ++C C C S F R S CR G CE+C+ Y + PC +C+CN GS SL CN G C C PGV G KC+ C +
Sbjct: 275 RCKCNGHASECVEGERGDLVCACQHHTVGADCQKCHP-FYQDRPWARATGDSANECLKCNCNGRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPED-PCLACNCNINGSVSLQCNAEGRCVCRPGVTGAKCNMCQDGF 424
BLAST of Laminin subunit alpha-2 vs. Ensembl Medaka
Match: lamc3 (laminin subunit gamma 3 [Source:NCBI gene;Acc:105355198]) HSP 1 Score: 367.466 bits (942), Expect = 1.264e-102 Identity = 307/1065 (28.83%), Postives = 463/1065 (43.47%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFA---KSDDDNLWWQSPSMAQGLKY-QNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYL---------EQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVA-----VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKP---PCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSS------LSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQY---LTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSL--IDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH--IEKCNCPPGFNGLSCESCESGY-RRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLD--YQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS----CKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS----CKKCDCDSLGSTSE--NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTD 3217
N + +S CG+ PE +C + S C CD + H +++ WWQS SM G++Y ++VN+T+ L + ++I YI LK S I +R+ +G ++P+ +++ SC + + + ++L CT +S L G + S + GR S N + + FL+ A DL+ L + + Y+Y++ + +GG+C CN HA +C +E C C+H+T G++C+ C P + RPW C +CNCN +S++C ++ + + GG C++C++NT+G +CE C YR +A E PC C C+ G++ S N E G C+C+ G G C++C G+ C PC C GSV C P C CK +V C CK G+FNL NP GC+PC+CFG + CSS ++ + I D W F GE+ L Y LT E+S ++ AP +LG+ I SYG E L SL I + D + + Q FI ++ E++ P +A E L + L I N S +L +TL+ + +P +E+C+CP GF+G CE C G+ R+ C C C+ H CD G C+ C+H T G CE C GYYG+A C+ CPCP Q+ E + VC C +G+ C C +GYYG+P + C RC CN N + CD TG+C +C +T G CQ C+ GFYG+AL ++ CK C C S G++ C TG C C ++ R C C G + C C C G S+ + C+ +G+C C V G C+ C G+F + + C+ C C P ++S C NG C+C + CDKCE ++Y++NR +
Sbjct: 59 NRTVEASNVCGST-PEDYC-----------MQTGSTRSCHYCDASDPELSHNASLLTDFHRNEEPTWWQSQSMFYGIQYPKSVNLTLHLGKAFEITYIRLKFYTSRPESFAIYKRTEEGGPWLPYQYYSA---SCKKTYGKDAKGFIRPGEEEKTAL------CTDEFSDISPLTGGNVAFSTLEGRPSAYN--------------------FDQSLFLQEWVTATDLLISLDRLNTFGDEFFKDAKVLRSYFYAISDFSVGGRCKCNGHASEC---VEGERGDLVCACQHHTVGADCQKCHPFYQDRPWARATGDSANECLKCNCNGRSDQCIFD---------MEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYR----VAPE-DPCLACNCNINGSV-SLQCNAE-------GRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGSVG----ECSPLDGQCFCKRNVEGHR-CGRCKPGFFNLQLQNPAGCQPCFCFGHSLACSSSNHYTPVNITSDFIEDQDRWVGEFFGGEEYILQWKEGEVYLLPLTEEDS-----------GFYKAPDKFLGHQIYSYGQPLHITFTSETYDLLPNSLTVILEGSGMTLSADLYPKPILHPDPSNSPMQHFI-VRLHEYET-SFKPRLSAF--ELQHLLYNLTALKISNAGGHNYTS-QLARVTLASASISSRPLSLPAAWVEQCSCPLGFSGQFCEGCAPGFTRQEPGGGPLSACVPCKCHQH-GICDPETGVCE-CSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPDRSSCVQIAE------TGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIG--STSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVSMQCH-GNGTCQCRQGFVGYKCDKCE-VNYFHNRAN 1024 HSP 2 Score: 127.102 bits (318), Expect = 2.596e-28 Identity = 143/573 (24.96%), Postives = 224/573 (39.09%), Query Frame = 2
Query: 3407 GICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY--GFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEK---NSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIIL--EDSNVINSNLTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKND--KNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIKGI----PCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSG 5077
G CV C NT G C C + + C CNC INGS S CN G C C+ G+KC+ C +G++ G C+PC C +G+ + +C + GQC CK+NV G +C C+ G F GC CFCFG S C + NH N ++ I D+ G++Y L + PLT + F H + + + + + + + D +P V +E GM + + N + FI+ E L+ L N+T+LK+ L ++ + + + +++ + VE+C C G C+ G+ ++ G L C C+ + +CD TG C+ C G C+ C G+Y N C+PC CP +C +I + ++ C C G +G C+ C+ G+YG+P+ PC C+CN +++ N GIC G +C +C + E CQ C G
Sbjct: 361 GRCVDCRDNTDGAHCEHCKANHYRVAPEDPCLACNCNINGSVSLQCNA-EGRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGS----VGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCFCFGHSLACSSS-NHYTPVNITSDF-IEDQDRWVGEFFGGEEYI---LQWKEGEVYLLPLTEEDSGFYKAPDKFLGHQIYSYGQPLHITFTSETYD-----LLPNSLTVILEGS----GMTLSADLYPK----------------PILHPDPSNSPMQHFIVRLHEYETSFKPRLSAFELQHLLYNLTALKISNAGGHNYTSQLARVTLASASI--SSRPLSLPAAWVEQCSCPLGFSGQFCEGCAPGFTRQ-------------EPGGGPLSACVPCKCHQHG-ICDPETGVCE-CSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPD-----RSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCN----GNVDFNAVGICDHFTG----QCLKCMGH--TEGDHCQRCQRG 866 HSP 3 Score: 119.398 bits (298), Expect = 5.555e-26 Identity = 99/338 (29.29%), Postives = 143/338 (42.31%), Query Frame = 2
Query: 2591 SGVHCDRCIEGYY-----GNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP-------NCKKCLCSPHALSA---DCDLNNGKCKCGSNVINRICDKCENLHYWNNRT--DCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC 3505
SG C+ C G+ G P +C CKC+ + CDP TG C CS+ T G C+ C G+YG+AL + C+ C C S + + C +C +C C G Y + C+ CNC ++ C+ +GQC C+ + GD C++C G++ CK C CS S +C G C C S+V R C CE + ++N + C C C GS + C TG C C V C C +G+FG GC+ C P C+ G C C +G C+KC + + C++C
Sbjct: 697 SGQFCEGCAPGFTRQEPGGGPLS--ACVPCKCHQHG-ICDPETGVCECSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPDRSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCE-VGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVSMQCHGNGTCQCRQGFVGYKCDKCEVNYFHNRANHQCEEC 1030 HSP 4 Score: 116.316 bits (290), Expect = 4.962e-25 Identity = 111/405 (27.41%), Postives = 167/405 (41.23%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSADCDLNNGKC---KCGSNVINRICDKCENLHYWNNRTD------CVPCNC-GRGSLNPK--CDMETGNC-KCAQNVARRDCSKCDVGYFG--LDVTGCKKCPPC------YNGQV--CNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC-FCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANY 3952
+C C F + C+ C G ++ C+PC C G C+ +G C C G CE C+ GY+ +C+ C C P S G+ C + C CE+ +Y + CV CNC G N CD TG C KC + C +C G++G L+ T +KC PC +G V C+ + G C C + G C+ C ++ GC++C C GS S C+ +G C C+ G+ CD C G++GF + C+ C+C G+ S +C G CQC+Q +G KC C F + ++ C C C+ + +K L R+ + H FD + L + H+ Y
Sbjct: 689 QCSCPLGFSGQFCEGCAPGFTRQEPGGGPLSACVPCKCHQHG------ICDPETGVCECSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPC-PDRSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVS---MQCHG-NGTCQCRQGFVGYKCDKCEVNYFHNRANHQ--CEECPVCYSLVKKQAEKLQTRLQDLEKLLSH-FDCRGRLGRQPNLQQYHMAGFY 1079 HSP 5 Score: 102.449 bits (254), Expect = 7.888e-21 Identity = 95/354 (26.84%), Postives = 141/354 (39.83%), Query Frame = 2
Query: 4484 CNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNG-----YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN---DKNYDQCEPCQCPSA----HYNFAQ------------------------------------NCTRIESSLEL------KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYSGCTKD---LLVRIDTLNETVINF----KWKTLPSTLDLRLNKINISIQMSSE--LLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYN 5341
C A G CQ GYY + +SG++R R CNCNG +CD TG C CMG+ EG+ CQ C+ GFY N + +C+PC C SA H N C+ I S+ + +C CR G G C++C+ GF+G +G C++C+C+ GS S+ C+ G CQC G +G KCD+C NY + + C CYS K L R+ L + + +F + P+ + Q+ E L N++ F + + F QF ++ ++ L N
Sbjct: 794 CPAGQTGMRCQMCEDGYYGDPMG-----QSGTVRPCVR-------CNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRG--CRACNCDPMGSVSMQCHGNGTCQCRQGFVGYKCDKCEVNYFHNRANHQCEECPVCYSLVKKQAEKLQTRLQDLEKLLSHFDCRGRLGRQPNLQQYHMAGFYQHDQLRGEDSLPNALEDFLAFQEAREAFIKQFSSLEAATGTLSAQLHGLTFTLN 1133 HSP 6 Score: 79.337 bits (194), Expect = 8.474e-14 Identity = 70/227 (30.84%), Postives = 97/227 (42.73%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-----CRCSYNTMGTECQLCKHGFYGDA--------LTKSCKKCDCDSLGSTSENC---------DKLTGKC-DCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--PNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY---WNNRTDCVPCNCGRGSL 3250
RCKCN + C V GE C C ++T+G +CQ C H FY D C KC+C+ S+ C G+C DCR N C+ C + Y + C+ CNC I G +S + CN G+C C V G +C C +G+ + C+ C C +C +G+C C NV C +C+ + N C PC C SL
Sbjct: 281 RCKCNGHASEC--VEGERGDLVCACQHHTVGADCQKC-HPFYQDRPWARATGDSANECLKCNCN---GRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPEDPCLACNCNINGSVSLQ--CN-AEGRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGSVGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCFCFGHSL 498 HSP 7 Score: 61.6178 bits (148), Expect = 1.869e-8 Identity = 60/213 (28.17%), Postives = 84/213 (39.44%), Query Frame = 2
Query: 4478 CICNALTFGNHCQ--NPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY----VVEKKTCQSCY 5071
C C T G CQ +P Y + P + +G + N L KCNCNG S C G C +C N +G C+ C+ Y+ D C C C ++ + E +C CR G +G C CQ GF+ G C+ C+C+ GS G C C V G +C +C P + + CQ C+
Sbjct: 301 CACQHHTVGADCQKCHPF------YQDRPWARATGD--SANECL----KCNCNGRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPE-DPCLACNCN------INGSVSLQCNAEGRCVCRPGVTGAKCNMCQDGFHSLGPSG--CRPCECHHSGSVGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCF 492 HSP 8 Score: 55.4546 bits (132), Expect = 1.251e-6 Identity = 49/152 (32.24%), Postives = 66/152 (43.42%), Query Frame = 2
Query: 4616 KCNCNGYSKVC-DSVTGDCQ-NCMGNREGNKCQLCREGFYKN-------DKNYDQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+C CNG++ C + GD C + G CQ C FY++ + ++C C C S F R S CR G CE+C+ Y + PC +C+CN GS SL CN G C C PGV G KC+ C +
Sbjct: 281 RCKCNGHASECVEGERGDLVCACQHHTVGADCQKCHP-FYQDRPWARATGDSANECLKCNCNGRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPED-PCLACNCNINGSVSLQCNAEGRCVCRPGVTGAKCNMCQDGF 430 HSP 9 Score: 53.5286 bits (127), Expect = 5.778e-6 Identity = 54/202 (26.73%), Postives = 86/202 (42.57%), Query Frame = 2
Query: 4478 CICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILI---NLKKCNCNGYS---KVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYD-QCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYS-GKSCENCQQGFYGNPIK---GIPCKSCDCNQQGSQSLNCNE----FGICQCSPGVLGIKCDQCAPNY 5038
C C+ T G C++ + GYY GN ++ + + C C S ++ ++ C NC + G +CQ+C +G+Y + PC + + N N I +C +G++ G C+ CQ+GFYGN ++ G CK C C+ G+ S + NE G C C V G C C +
Sbjct: 739 CECSHFTTGMTCEHCLPGYY-----------------GNALIGTPGDCQPCPCPDRSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGT-SGHVNECHPQTGSCHCLSHVTGRDCSYCEVGF 922
BLAST of Laminin subunit alpha-2 vs. Ensembl Medaka
Match: lamc3 (laminin subunit gamma 3 [Source:NCBI gene;Acc:105355198]) HSP 1 Score: 367.851 bits (943), Expect = 1.993e-102 Identity = 307/1065 (28.83%), Postives = 463/1065 (43.47%), Query Frame = 2
Query: 197 NAKITSSATCGTGGPEIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFA---KSDDDNLWWQSPSMAQGLKY-QNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLDGEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYL---------EQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVA-----VCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKP---PCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSS------LSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQY---LTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSL--IDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPH--IEKCNCPPGFNGLSCESCESGY-RRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLD--YQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS----CKRCKCNNNQES-----CDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS----CKKCDCDSLGSTSE--NCDKLTGKCDCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPN--CKKCLCSPH-ALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTD 3217
N + +S CG+ PE +C + S C CD + H +++ WWQS SM G++Y ++VN+T+ L + ++I YI LK S I +R+ +G ++P+ +++ SC + + + ++L CT +S L G + S + GR S N + + FL+ A DL+ L + + Y+Y++ + +GG+C CN HA +C +E C C+H+T G++C+ C P + RPW C +CNCN +S++C ++ + + GG C++C++NT+G +CE C YR +A E PC C C+ G++ S N E G C+C+ G G C++C G+ C PC C GSV C P C CK +V C CK G+FNL NP GC+PC+CFG + CSS ++ + I D W F GE+ L Y LT E+S ++ AP +LG+ I SYG E L SL I + D + + Q FI ++ E++ P +A E L + L I N S +L +TL+ + +P +E+C+CP GF+G CE C G+ R+ C C C+ H CD G C+ C+H T G CE C GYYG+A C+ CPCP Q+ E + VC C +G+ C C +GYYG+P + C RC CN N + CD TG+C +C +T G CQ C+ GFYG+AL ++ CK C C S G++ C TG C C ++ R C C G + C C C G S+ + C+ +G+C C V G C+ C G+F + + C+ C C P ++S C NG C+C + CDKCE ++Y++NR +
Sbjct: 59 NRTVEASNVCGST-PEDYC-----------MQTGSTRSCHYCDASDPELSHNASLLTDFHRNEEPTWWQSQSMFYGIQYPKSVNLTLHLGKAFEITYIRLKFYTSRPESFAIYKRTEEGGPWLPYQYYSA---SCKKTYGKDAKGFIRPGEEEKTAL------CTDEFSDISPLTGGNVAFSTLEGRPSAYN--------------------FDQSLFLQEWVTATDLLISLDRLNTFGDEFFKDAKVLRSYFYAISDFSVGGRCKCNGHASEC---VEGERGDLVCACQHHTVGADCQKCHPFYQDRPWARATGDSANECLKCNCNGRSDQCIFD---------MEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYR----VAPE-DPCLACNCNINGSV-SLQCNAE-------GRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGSVG----ECSPLDGQCFCKRNVEGHR-CGRCKPGFFNLQLQNPAGCQPCFCFGHSLACSSSNHYTPVNITSDFIEDQDRWVGEFFGGEEYILQWKEGEVYLLPLTEEDS-----------GFYKAPDKFLGHQIYSYGQPLHITFTSETYDLLPNSLTVILEGSGMTLSADLYPKPILHPDPSNSPMQHFI-VRLHEYET-SFKPRLSAF--ELQHLLYNLTALKISNAGGHNYTS-QLARVTLASASISSRPLSLPAAWVEQCSCPLGFSGQFCEGCAPGFTRQEPGGGPLSACVPCKCHQH-GICDPETGVCE-CSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPDRSSCVQIAE------TGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIG--STSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVSMQCH-GNGTCQCRQGFVGYKCDKCE-VNYFHNRAN 1024 HSP 2 Score: 127.102 bits (318), Expect = 2.517e-28 Identity = 143/573 (24.96%), Postives = 224/573 (39.09%), Query Frame = 2
Query: 3407 GICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY--GFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEK---NSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIIL--EDSNVINSNLTRDIFMMALLNVTSLKVK-LYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKND--KNYDQCEPCQCPSAHYNFAQNCTRIESSLELKC-QCRIGYSGKSCENCQQGFYGNPIKGI----PCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSG 5077
G CV C NT G C C + + C CNC INGS S CN G C C+ G+KC+ C +G++ G C+PC C +G+ + +C + GQC CK+NV G +C C+ G F GC CFCFG S C + NH N ++ I D+ G++Y L + PLT + F H + + + + + + + D +P V +E GM + + N + FI+ E L+ L N+T+LK+ L ++ + + + +++ + VE+C C G C+ G+ ++ G L C C+ + +CD TG C+ C G C+ C G+Y N C+PC CP +C +I + ++ C C G +G C+ C+ G+YG+P+ PC C+CN +++ N GIC G +C +C + E CQ C G
Sbjct: 361 GRCVDCRDNTDGAHCEHCKANHYRVAPEDPCLACNCNINGSVSLQCNA-EGRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGS----VGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCFCFGHSLACSSS-NHYTPVNITSDF-IEDQDRWVGEFFGGEEYI---LQWKEGEVYLLPLTEEDSGFYKAPDKFLGHQIYSYGQPLHITFTSETYD-----LLPNSLTVILEGS----GMTLSADLYPK----------------PILHPDPSNSPMQHFIVRLHEYETSFKPRLSAFELQHLLYNLTALKISNAGGHNYTSQLARVTLASASI--SSRPLSLPAAWVEQCSCPLGFSGQFCEGCAPGFTRQ-------------EPGGGPLSACVPCKCHQHG-ICDPETGVCE-CSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPD-----RSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCN----GNVDFNAVGICDHFTG----QCLKCMGH--TEGDHCQRCQRG 866 HSP 3 Score: 119.398 bits (298), Expect = 4.814e-26 Identity = 99/338 (29.29%), Postives = 143/338 (42.31%), Query Frame = 2
Query: 2591 SGVHCDRCIEGYY-----GNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKS---CKKCDCDSLGSTSENCDKLTGKC-DCRTNFVNRQCDSCTSGLYLKD-------KKCIPCNCEIQGRISSEKFCNTTSGQCF-CLENVNGDRCEKCVEGYFNYP-------NCKKCLCSPHALSA---DCDLNNGKCKCGSNVINRICDKCENLHYWNNRT--DCVPCNCGR-GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC---PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKC 3505
SG C+ C G+ G P +C CKC+ + CDP TG C CS+ T G C+ C G+YG+AL + C+ C C S + + C +C +C C G Y + C+ CNC ++ C+ +GQC C+ + GD C++C G++ CK C CS S +C G C C S+V R C CE + ++N + C C C GS + C TG C C V C C +G+FG GC+ C P C+ G C C +G C+KC + + C++C
Sbjct: 697 SGQFCEGCAPGFTRQEPGGGPLS--ACVPCKCHQHG-ICDPETGVCECSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPCPDRSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCE-VGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVSMQCHGNGTCQCRQGFVGYKCDKCEVNYFHNRANHQCEEC 1030 HSP 4 Score: 116.701 bits (291), Expect = 3.309e-25 Identity = 111/405 (27.41%), Postives = 167/405 (41.23%), Query Frame = 2
Query: 2855 KCDCRTNFVNRQCDSCTSGLYLKD------KKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYP------NCKKCLCSPHALSADCDLNNGKC---KCGSNVINRICDKCENLHYWNNRTD------CVPCNC-GRGSLNPK--CDMETGNC-KCAQNVARRDCSKCDVGYFG--LDVTGCKKCPPC------YNGQV--CNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN--CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSC-FCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANY 3952
+C C F + C+ C G ++ C+PC C G C+ +G C C G CE C+ GY+ +C+ C C P S G+ C + C CE+ +Y + CV CNC G N CD TG C KC + C +C G++G L+ T +KC PC +G V C+ + G C C + G C+ C ++ GC++C C GS S C+ +G C C+ G+ CD C G++GF + C+ C+C G+ S +C G CQC+Q +G KC C F + ++ C C C+ + +K L R+ + H FD + L + H+ Y
Sbjct: 689 QCSCPLGFSGQFCEGCAPGFTRQEPGGGPLSACVPCKCHQHG------ICDPETGVCECSHFTTGMTCEHCLPGYYGNALIGTPGDCQPCPC-PDRSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRGCRACNCDPMGSVS---MQCHG-NGTCQCRQGFVGYKCDKCEVNYFHNRANHQ--CEECPVCYSLVKKQAEKLQTRLQDLEKLLSH-FDCRGRLGRQPNLQQYHMAGFY 1079 HSP 5 Score: 102.449 bits (254), Expect = 7.335e-21 Identity = 93/336 (27.68%), Postives = 137/336 (40.77%), Query Frame = 2
Query: 4484 CNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNG-----YSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKN---DKNYDQCEPCQCPSA----HYNFAQ------------------------------------NCTRIESSLEL------KCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEK-----KTCQSCYSGCTKD---LLVRIDTLNETVINF----KWKTLPSTLDLRLNKINISIQMSSE--LLNSINKFYKLKNEIKNFSIQFQKMK 5287
C A G CQ GYY + +SG++R R CNCNG +CD TG C CMG+ EG+ CQ C+ GFY N + +C+PC C SA H N C+ I S+ + +C CR G G C++C+ GF+G +G C++C+C+ GS S+ C+ G CQC G +G KCD+C NY + + C CYS K L R+ L + + +F + P+ + Q+ E L N++ F + + F QF ++
Sbjct: 794 CPAGQTGMRCQMCEDGYYGDPMG-----QSGTVRPCVR-------CNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGTSGHVNECHPQTGSCHCLSHVTGRDCSYCEVGFFNLQPGYGCERCKCSPIGSTSQACHPFTGECVCRPGVEGTLCDSCRLGFFGFSSRG--CRACNCDPMGSVSMQCHGNGTCQCRQGFVGYKCDKCEVNYFHNRANHQCEECPVCYSLVKKQAEKLQTRLQDLEKLLSHFDCRGRLGRQPNLQQYHMAGFYQHDQLRGEDSLPNALEDFLAFQEAREAFIKQFSSLE 1115 HSP 6 Score: 79.7221 bits (195), Expect = 6.674e-14 Identity = 70/227 (30.84%), Postives = 97/227 (42.73%), Query Frame = 2
Query: 2660 RCKCNNNQESCDPVTGE-----CRCSYNTMGTECQLCKHGFYGDA--------LTKSCKKCDCDSLGSTSENC---------DKLTGKC-DCRTNFVNRQCDSCTSGLY--LKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNY--PNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHY---WNNRTDCVPCNCGRGSL 3250
RCKCN + C V GE C C ++T+G +CQ C H FY D C KC+C+ S+ C G+C DCR N C+ C + Y + C+ CNC I G +S + CN G+C C V G +C C +G+ + C+ C C +C +G+C C NV C +C+ + N C PC C SL
Sbjct: 281 RCKCNGHASEC--VEGERGDLVCACQHHTVGADCQKC-HPFYQDRPWARATGDSANECLKCNCN---GRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPEDPCLACNCNINGSVSLQ--CN-AEGRCVCRPGVTGAKCNMCQDGFHSLGPSGCRPCECHHSGSVGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCFCFGHSL 498 HSP 7 Score: 61.6178 bits (148), Expect = 1.649e-8 Identity = 60/213 (28.17%), Postives = 84/213 (39.44%), Query Frame = 2
Query: 4478 CICNALTFGNHCQ--NPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC---------DSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY----VVEKKTCQSCY 5071
C C T G CQ +P Y + P + +G + N L KCNCNG S C G C +C N +G C+ C+ Y+ D C C C ++ + E +C CR G +G C CQ GF+ G C+ C+C+ GS G C C V G +C +C P + + CQ C+
Sbjct: 301 CACQHHTVGADCQKCHPF------YQDRPWARATGD--SANECL----KCNCNGRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPE-DPCLACNCN------INGSVSLQCNAEGRCVCRPGVTGAKCNMCQDGFHSLGPSG--CRPCECHHSGSVGECSPLDGQCFCKRNVEGHRCGRCKPGFFNLQLQNPAGCQPCF 492 HSP 8 Score: 55.8398 bits (133), Expect = 1.066e-6 Identity = 49/152 (32.24%), Postives = 66/152 (43.42%), Query Frame = 2
Query: 4616 KCNCNGYSKVC-DSVTGDCQ-NCMGNREGNKCQLCREGFYKN-------DKNYDQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
+C CNG++ C + GD C + G CQ C FY++ + ++C C C S F R S CR G CE+C+ Y + PC +C+CN GS SL CN G C C PGV G KC+ C +
Sbjct: 281 RCKCNGHASECVEGERGDLVCACQHHTVGADCQKCHP-FYQDRPWARATGDSANECLKCNCNGRSDQCIFDMEQYRSTGSGGRCVDCRDNTDGAHCEHCKANHYRVAPED-PCLACNCNINGSVSLQCNAEGRCVCRPGVTGAKCNMCQDGF 430 HSP 9 Score: 53.5286 bits (127), Expect = 5.005e-6 Identity = 54/202 (26.73%), Postives = 86/202 (42.57%), Query Frame = 2
Query: 4478 CICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILI---NLKKCNCNGYS---KVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYD-QCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYS-GKSCENCQQGFYGNPIK---GIPCKSCDCNQQGSQSLNCNE----FGICQCSPGVLGIKCDQCAPNY 5038
C C+ T G C++ + GYY GN ++ + + C C S ++ ++ C NC + G +CQ+C +G+Y + PC + + N N I +C +G++ G C+ CQ+GFYGN ++ G CK C C+ G+ S + NE G C C V G C C +
Sbjct: 739 CECSHFTTGMTCEHCLPGYY-----------------GNALIGTPGDCQPCPCPDRSSCVQIAETGQVVCTNCPAGQTGMRCQMCEDGYYGDPMGQSGTVRPCVRCNCNGNVDFNAVGICDHFTGQCLKCMGHTEGDHCQRCQRGFYGNALEQTSGQKCKPCSCSSAGT-SGHVNECHPQTGSCHCLSHVTGRDCSYCEVGF 922
BLAST of Laminin subunit alpha-2 vs. Ensembl Medaka
Match: ENSORLT00000031008.1 (pep primary_assembly:ASM223467v1:17:25500792:25530071:1 gene:ENSORLG00000029606.1 transcript:ENSORLT00000031008.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 309.301 bits (791), Expect = 3.800e-91 Identity = 182/539 (33.77%), Postives = 277/539 (51.39%), Query Frame = 2
Query: 83 HLKMRIFIIFILLIVHKAHSQDDEPEILSVNVDGL---LMENAKITSSATCG---TGGP--EIFCRLVDEIAIQRLINPDSHVLCDVCDNRNETKRHPIEFAKSDDDNLWWQSPSMAQGLKYQNVNVTIDLKQVYQIVYILLKMGDSPRPGNWILERSLD-GEIYMPWIFFAETPYSCYNLFQRQYPDLKLTTNAKPSSLADDEMFCTTYYSQPKSLNSGEIIISLINGRKSIMNEEYLSIKSVPQTLINFLSARYIRFRFLKHLTLAADLMTHKGYLEQSIYNRYYYSLRNIEIGGKCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETCCPLFNQRPWKPVAV-----CEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPN---------------GECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNL 1612
HL + +F+ ++ A Q+ ++ +++ L E KI++SATCG G P +++C+LV CD C++ + +K HP A D WWQSP +++G Y VNVT+DL Q++ + Y+L+K +SPRP W+LERS+D G + PW FFA + C F + P+ ++ L DD+ CTT YS+ L +GEI++SLINGR N Y + L F A IR RFL+ TL L++ K + S+ RYYYS+++I +GG+C+C+ HA ++ KC C+HNTCG +C+ CCP FNQ+PW+ C C C +++C Y+ V S++ GR GGGVC++CQ NT G+NCE+C +GF+RP++V C C C ++G ++G G C+C+ + G NCD C+ GY YP G + C C G+V + C C+ V+ + C C+ GY++
Sbjct: 9 HL-LAVFLTSWATVLRDAGGQEPFNDLTGFSLNPPYFNLAEGIKISASATCGQDEAGAPRQDLYCKLVGGPNYGLSNQHIQGQFCDHCNSADPSKAHPATNAI-DGTERWWQSPPLSRGSGYNEVNVTLDLGQLFHVAYVLIKFANSPRPDLWVLERSVDNGRTFTPWQFFAHSKRECIERFGKP-PNARV--------LKDDDQLCTTEYSRIIPLENGEIVVSLINGRPGSKNFTYSPV------LQQFTKATNIRLRFLRTSTLLGHLIS-KAQRDPSVTRRYYYSIKDISVGGRCVCHGHAQ---------VNREKCECQHNTCGESCDRCCPGFNQKPWRAATADSPNECRPCQCFSHASDCYYDPEVERRGASLDTFGRYSGGGVCVDCQHNTAGVNCERCAEGFFRPYDVPPESHTGCISCSCDDR------TASGCEMGS---GRCVCRPQFAGENCDRCADGYYLYPQCISYPVRLTTTESPAGPIVACSCDYRGTV-SRVCDASGRCLCREGVDG-ERCDRCRRGYYSF 509 HSP 2 Score: 95.1301 bits (235), Expect = 3.914e-19 Identity = 68/229 (29.69%), Postives = 104/229 (45.41%), Query Frame = 2
Query: 2534 LCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKIS------CKRCKCNNNQESC--DPVT----------------GEC-RCSYNTMGTECQLCKHGFYG--DALTKS---CKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDK----------------KCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCK 3082
+C ++ N +C HNT G CDRC G+ P + + C+ C+C ++ C DP G C C +NT G C+ C GF+ D +S C C CD T+ C+ +G+C CR F CD C G YL + + C+C+ +G +S + C+ SG+C C E V+G+RC++C GY+++PNC+
Sbjct: 290 VCHGHAQVNREKCECQHNTCGESCDRCCPGFNQKPWRAATADSPNECRPCQCFSHASDCYYDPEVERRGASLDTFGRYSGGGVCVDCQHNTAGVNCERCAEGFFRPYDVPPESHTGCISCSCDD--RTASGCEMGSGRCVCRPQFAGENCDRCADGYYLYPQCISYPVRLTTTESPAGPIVACSCDYRGTVS--RVCDA-SGRCLCREGVDGERCDRCRRGYYSFPNCQ 513 HSP 3 Score: 72.4034 bits (176), Expect = 5.793e-12 Identity = 78/262 (29.77%), Postives = 106/262 (40.46%), Query Frame = 2
Query: 3083 KCLCSPHALSADCDLNNGKCKCGSNVINRICDKC---ENLHYWNNRT-----DCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICV-CPPNTIGNLCNKCSEDSWDFNET-----IGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNC-----------------QPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKG--TFP 3769
+C+C HA +N KC+C N CD+C N W T +C PC +C +C V RR S LD G Y+G G+CV C NT G C +C+E + + GC C+C + ++ C SG C C+ F G CDRC +GYY +P C CSC GT S CD +G+C C++ V G +C CR+G +FP
Sbjct: 288 RCVCHGHA-----QVNREKCECQHNTCGESCDRCCPGFNQKPWRAATADSPNECRPC---------QCFSHASDCYYDPEVERRGAS--------LDTFGR------YSGG-----GVCVDCQHNTAGVNCERCAEGFFRPYDVPPESHTGCISCSC--DDRTASGCEMGSGRCVCRPQFAGENCDRCADGYYLYPQCISYPVRLTTTESPAGPIVACSCDYRGTVSR---VCDA-SGRCLCREGVDGERCDRCRRGYYSFP 510 HSP 4 Score: 62.003 bits (149), Expect = 9.161e-9 Identity = 63/250 (25.20%), Postives = 92/250 (36.80%), Query Frame = 2
Query: 2141 IKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYR----RVNNVLYQGQCEACACYNHSNTCD------------------SLNGECQNCTHNTAGAHCEDCASGYYG--DAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPK-------------KKISCKRCKCNNN---QESCDPVTGECRCSYNTMGTECQLCKHGFY 2770
+ +YY + K I + + G + + EKC C G SC+ C G+ R +C C C++H++ C S G C +C HNTAG +CE CA G++ D ++ C C C D + C S C +G +CDRC +GYY P+ C C+ CD +G C C G C C+ G+Y
Sbjct: 272 VTRRYYYSIKDISVGGRCVCHGHAQV------NREKCECQHNTCGESCDRCCPGFNQKPWRAATADSPNECRPCQCFSHASDCYYDPEVERRGASLDTFGRYSGGGVCVDCQHNTAGVNCERCAEGFFRPYDVPPESHTGCISCSC---DDRTASGCEMGSGRCV----CRPQFAGENCDRCADGYYLYPQCISYPVRLTTTESPAGPIVACSCDYRGTVSRVCD-ASGRCLCREGVDGERCDRCRRGYY 507 HSP 5 Score: 56.9954 bits (136), Expect = 3.287e-7 Identity = 42/148 (28.38%), Postives = 66/148 (44.59%), Query Frame = 2
Query: 4652 SVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCT---RIESSLEL---KCQCRIGYSGKSCENCQQGFY------GNPIK-------GIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNY 5038
S G C +C N G C+ C EGF++ P P + +C+ R S E+ +C CR ++G++C+ C G+Y P++ P +C C+ +G+ S C+ G C C GV G +CD+C Y
Sbjct: 368 SGGGVCVDCQHNTAGVNCERCAEGFFR---------PYDVPPESHTGCISCSCDDRTASGCEMGSGRCVCRPQFAGENCDRCADGYYLYPQCISYPVRLTTTESPAGPIVACSCDYRGTVSRVCDASGRCLCREGVDGERCDRCRRGY 506
BLAST of Laminin subunit alpha-2 vs. Planmine SMEST
Match: SMESG000056013.1 (SMESG000056013.1) HSP 1 Score: 5771.82 bits (14972), Expect = 0.000e+0 Identity = 2850/2858 (99.72%), Postives = 2853/2858 (99.83%), Query Frame = 2
Query: 1253 EKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNASDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLLTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 9826
KCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLN CKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTG+CDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLE+VNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNK+LEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENN ILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQN SDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKL TIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY
Sbjct: 27 RKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKILEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENNLILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNTSDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLFTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 2884 HSP 2 Score: 75.0998 bits (183), Expect = 1.373e-12 Identity = 78/295 (26.44%), Postives = 114/295 (38.64%), Query Frame = 2
Query: 914 YYSLRNIEIGGKC---ICNNHADKCDKVIENGISVAKC-NCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYR-----------------------------PWNVLATE-------------LYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFY--RCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
Y + N+ G+C C NH++ CD + NG +C NC HNT G++CE C + K + C++C C R Y T S VC C NT G++C++CI+G+Y +N + TE C C C LG S E+ D+ G+C C+ + CD C+ G + +C+PC C I G + + + C C VN D C+ C GYFN + C+ C C
Sbjct: 377 YRRVNNVLYQGQCEACACYNHSNTCDSL--NG----ECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPR----LDYQMTELCSSYSSENY-------VCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLG------STSENC-DKLTGECDCRTNFVNRQCDSCTSGL-YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDR-CEKCVEGYFNYPN-----CKKCLC 640 HSP 3 Score: 63.929 bits (154), Expect = 2.626e-9 Identity = 68/266 (25.56%), Postives = 96/266 (36.09%), Query Frame = 2
Query: 947 KCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETC-CPLFNQRPWKPVAVCE----------------ECNC-----NRKSNECS---YNQTVATLQLSVNKMGRMEGGGVCIN------CQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCY 1648
+C CNN+ + CD V +C C +NT G+ C+ C + K C+ EC+C NR+ + C+ Y + + + GR+ C C E+ G CEKC++G++ YP C C C C D G C C CD C + +C+PC C GS+ C C +V D C C GYF LD + C PCY
Sbjct: 496 RCKCNNNQESCDPV------TGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFN---------YPNCKKCLCSPHALSADC--------DLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRD-CSKCDVGYFGLDVTGCKKCPPCY 736
BLAST of Laminin subunit alpha-2 vs. Planmine SMEST
Match: SMESG000056013.1 (SMESG000056013.1) HSP 1 Score: 5768.35 bits (14963), Expect = 0.000e+0 Identity = 2850/2858 (99.72%), Postives = 2853/2858 (99.83%), Query Frame = 2
Query: 1253 EKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNASDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLLTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 9826
KCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLN CKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTG+CDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLE+VNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNK+LEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENN ILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQN SDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKL TIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY
Sbjct: 9 RKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKILEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENNLILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNTSDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLFTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 2866 HSP 2 Score: 74.7146 bits (182), Expect = 1.810e-12 Identity = 78/295 (26.44%), Postives = 114/295 (38.64%), Query Frame = 2
Query: 914 YYSLRNIEIGGKC---ICNNHADKCDKVIENGISVAKC-NCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYR-----------------------------PWNVLATE-------------LYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFY--RCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
Y + N+ G+C C NH++ CD + NG +C NC HNT G++CE C + K + C++C C R Y T S VC C NT G++C++CI+G+Y +N + TE C C C LG S E+ D+ G+C C+ + CD C+ G + +C+PC C I G + + + C C VN D C+ C GYFN + C+ C C
Sbjct: 359 YRRVNNVLYQGQCEACACYNHSNTCDSL--NG----ECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPR----LDYQMTELCSSYSSENY-------VCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLG------STSENC-DKLTGECDCRTNFVNRQCDSCTSGL-YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDR-CEKCVEGYFNYPN-----CKKCLC 622 HSP 3 Score: 63.5438 bits (153), Expect = 3.490e-9 Identity = 68/266 (25.56%), Postives = 96/266 (36.09%), Query Frame = 2
Query: 947 KCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETC-CPLFNQRPWKPVAVCE----------------ECNC-----NRKSNECS---YNQTVATLQLSVNKMGRMEGGGVCIN------CQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCY 1648
+C CNN+ + CD V +C C +NT G+ C+ C + K C+ EC+C NR+ + C+ Y + + + GR+ C C E+ G CEKC++G++ YP C C C C D G C C CD C + +C+PC C GS+ C C +V D C C GYF LD + C PCY
Sbjct: 478 RCKCNNNQESCDPV------TGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFN---------YPNCKKCLCSPHALSADC--------DLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRD-CSKCDVGYFGLDVTGCKKCPPCY 718
BLAST of Laminin subunit alpha-2 vs. Planmine SMEST
Match: SMESG000056013.1 (SMESG000056013.1) HSP 1 Score: 5767.19 bits (14960), Expect = 0.000e+0 Identity = 2850/2857 (99.75%), Postives = 2853/2857 (99.86%), Query Frame = 2
Query: 1256 KCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNASDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLLTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 9826
KCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLN CKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTG+CDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLE+VNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNK+LEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENN ILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQN SDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKL TIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY
Sbjct: 43 KCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKILEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENNLILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNTSDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLFTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 2899 HSP 2 Score: 74.3294 bits (181), Expect = 2.072e-12 Identity = 77/292 (26.37%), Postives = 112/292 (38.36%), Query Frame = 2
Query: 914 YYSLRNIEIGGKC---ICNNHADKCDKVIENGISVAKC-NCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYR-----------------------------PWNVLATE-------------LYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFY--RCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEP 1642
Y + N+ G+C C NH++ CD + NG +C NC HNT G++CE C + K + C++C C R Y T S VC C NT G++C++CI+G+Y +N + TE C C C LG S E+ D+ G+C C+ + CD C+ G + +C+PC C I G + + + C C VN D C+ C GYFN + C P
Sbjct: 392 YRRVNNVLYQGQCEACACYNHSNTCDSL--NG----ECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPR----LDYQMTELCSSYSSENY-------VCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLG------STSENC-DKLTGECDCRTNFVNRQCDSCTSGL-YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDR-CEKCVEGYFNYPNCKKCLCSP 657 HSP 3 Score: 63.5438 bits (153), Expect = 3.674e-9 Identity = 68/266 (25.56%), Postives = 96/266 (36.09%), Query Frame = 2
Query: 947 KCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETC-CPLFNQRPWKPVAVCE----------------ECNC-----NRKSNECS---YNQTVATLQLSVNKMGRMEGGGVCIN------CQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCY 1648
+C CNN+ + CD V +C C +NT G+ C+ C + K C+ EC+C NR+ + C+ Y + + + GR+ C C E+ G CEKC++G++ YP C C C C D G C C CD C + +C+PC C GS+ C C +V D C C GYF LD + C PCY
Sbjct: 511 RCKCNNNQESCDPV------TGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFN---------YPNCKKCLCSPHALSADC--------DLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRD-CSKCDVGYFGLDVTGCKKCPPCY 751
BLAST of Laminin subunit alpha-2 vs. Planmine SMEST
Match: SMESG000056013.1 (SMESG000056013.1) HSP 1 Score: 5736.38 bits (14880), Expect = 0.000e+0 Identity = 2838/2858 (99.30%), Postives = 2841/2858 (99.41%), Query Frame = 2
Query: 1253 EKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNASDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLLTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 9826
KCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLN CKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTG+CDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLE+VNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNK+LEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENN ILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCK NIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQN SDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKL TIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY
Sbjct: 3 RKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKILEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENNLILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKL-----------NIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNTSDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLFTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 2849 HSP 2 Score: 74.3294 bits (181), Expect = 2.139e-12 Identity = 78/295 (26.44%), Postives = 114/295 (38.64%), Query Frame = 2
Query: 914 YYSLRNIEIGGKC---ICNNHADKCDKVIENGISVAKC-NCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYR-----------------------------PWNVLATE-------------LYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFY--RCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
Y + N+ G+C C NH++ CD + NG +C NC HNT G++CE C + K + C++C C R Y T S VC C NT G++C++CI+G+Y +N + TE C C C LG S E+ D+ G+C C+ + CD C+ G + +C+PC C I G + + + C C VN D C+ C GYFN + C+ C C
Sbjct: 353 YRRVNNVLYQGQCEACACYNHSNTCDSL--NG----ECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPR----LDYQMTELCSSYSSENY-------VCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLG------STSENC-DKLTGECDCRTNFVNRQCDSCTSGL-YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDR-CEKCVEGYFNYPN-----CKKCLC 616 HSP 3 Score: 63.5438 bits (153), Expect = 3.825e-9 Identity = 68/266 (25.56%), Postives = 96/266 (36.09%), Query Frame = 2
Query: 947 KCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETC-CPLFNQRPWKPVAVCE----------------ECNC-----NRKSNECS---YNQTVATLQLSVNKMGRMEGGGVCIN------CQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCY 1648
+C CNN+ + CD V +C C +NT G+ C+ C + K C+ EC+C NR+ + C+ Y + + + GR+ C C E+ G CEKC++G++ YP C C C C D G C C CD C + +C+PC C GS+ C C +V D C C GYF LD + C PCY
Sbjct: 472 RCKCNNNQESCDPV------TGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFN---------YPNCKKCLCSPHALSADC--------DLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRD-CSKCDVGYFGLDVTGCKKCPPCY 712
BLAST of Laminin subunit alpha-2 vs. Planmine SMEST
Match: SMESG000056013.1 (SMESG000056013.1) HSP 1 Score: 5734.84 bits (14876), Expect = 0.000e+0 Identity = 2838/2858 (99.30%), Postives = 2841/2858 (99.41%), Query Frame = 2
Query: 1253 EKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNSCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGKCDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLENVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYXXXXXXXXXXXXXXDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKVLEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYXXXXXXXXXXXXSSEIPTGFYDVEILKRNLSXXXXXXXXXXXXFILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKFNPNSSIIFLDSNIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNASDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLLTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 9826
KCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLN CKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTG+CDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLE+VNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNK+LEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENN ILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCK NIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQN SDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKL TIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY
Sbjct: 9 RKCIKGFYRPWNVLATELYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYCFGITNQCSSLSAKNLKISDLKGWKLTTFTGEKIALPKFTSFQYLTSENSYIDERFNNPTVYYWTAPPPYLGNWITSYGTIFRYVVKIELQSTLKGSLIDSPDVIIKCGDFRLFHSVSIEDRRLQRQVFIYIKPTEWKIGKLTPDKAATKEEFISCLVKVDKLLIKAKYYSNQKSIELLNITLSRGEVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCGRGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVSEKCHAILNHRIVENSIYEYHIFDEKNSLESGKDYSTIHLNANYPRRFGWPLTMFRPIYVNVMKLFNSHDLSLFYRSIFMNVSVDCLDNKCGREIPEQTEVFMESYNGLFGMRYIVNHSVSNSNNIIVDFNEHSKNGKWVISQYQNDILDKFIILEDSNVINSNLTRDIFMMALLNVTSLKVKLYTDKVNPPLIKIKIITYKVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNEFGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNEIKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKILEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTIMADEINTNNKEYLNTMSTKKKEHELYIILINERMKDLQKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDLISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITRDILAVDYAINQTKKELDFNRSNMNIDSLKKLLAEFNSNKSEIVENIKNIDKEHEKLLKQYKNCSYNEKKLKNMNEKLSSEIPTGFYDVEILKRNLSEIQMEQNNIENNLILPINQTILNLGTPNVKSGLDTRNLEKLIKVVDNQYRDLVNESSFVENKIKDLRKSIKSLRERLQKARVMSELIHGAAARSVEPIRHVVRCVRHYVTYYLPTSNAFSLEFYMKPQILNDQSLVLFGYDDSGSIPHAFAFTLERNNQYKFWYDSGNSVESIELTGKFSNENYVGIMITWKLGKWHMVIRSISDGRTEQVETSSITNNILSNVRIGPQTNIFVGGFPSSKLLRSLKLEGRLISDLKENYDNYFYKGCLFGVKLMNTEFGLQDIEAIEEECRNTISIDNLLLGKFNCKL-----------NIESVNTFKRSVRRQKRDVQTNFDLHFTGNGFAMINTSRTKNGKCATSLNKVLTISTMALPKTHPDTFQLIAVFANKIMKYGFVLGQKNNSYHFGYWKKDVDYPLFPIQNTSDSFTWFIFKSKIEKLSETLFGIDEICDHNSLFYLSSIPDNDSYLIEKLKKLKVPNYGFTGKMKFLINKKPLFQVLATRFINVIFLFTEQVRENKFHSLLISPNAINPIEITEHSPNDILQNGFGFTVNFMGQPIAKTLKLFTIELEAGSVNVEVSRALVFSVIVNDVKIDKELQKFNNDNLDWEAHYIVVFLEQKQSSIIVTFTVDNEDIKSQEIMINIGSVNRMTLNPSSTNNHSYYIYNLFIGQKLVNFNELNYLPPGVFLKMRPTNLQPVDIQNPRVDTDKSIPRVSYKHGQNLQRDAVALPYIKSIPLYTNSNINAPFPKHKVKTLNLYSFKSQSYPAHSAWLAGISSNVLKLDASILNQYQITENNEISLKILSGSKRPEILMSLNYGTDKILNILTFNGQLILNNPSTKWTAELPILLINEQWHTLRITSNPLNSSTGVRVYQDNHSFNFQSLGFTITFPSKVELGGFSQTNPVFSYKDKNYMLKPFTGCLDELQINNNTVSILNSGSSNSVKQCPRVLELLNSGGQILAPYAEVTSKPIQLIFEPNQKFETDYFIKFKFKYTGYESGIILTIGDGKRFCSISIKNQEIQLIYLNMINKEKAVEKVEIDKERWYKIIFVVYHQADVLTICRMSINDDKIVRLHTLADSQMFDSNYKMSNLYFGYHPGLLADTKLVSEWDKNFVGCISDVECFVGNVPVSCQFDNSEYFIRGRCSIDKNTYLLRKY 2855 HSP 2 Score: 74.3294 bits (181), Expect = 2.104e-12 Identity = 78/295 (26.44%), Postives = 114/295 (38.64%), Query Frame = 2
Query: 914 YYSLRNIEIGGKC---ICNNHADKCDKVIENGISVAKC-NCKHNTCGSNCETCCP-LFNQRPWKPVAVCEECNCNRKSNECSYNQTVATLQLSVNKMGRMEGGGVCINCQENTEGINCEKCIKGFYR-----------------------------PWNVLATE-------------LYPCTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFY--RCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 1651
Y + N+ G+C C NH++ CD + NG +C NC HNT G++CE C + K + C++C C R Y T S VC C NT G++C++CI+G+Y +N + TE C C C LG S E+ D+ G+C C+ + CD C+ G + +C+PC C I G + + + C C VN D C+ C GYFN + C+ C C
Sbjct: 359 YRRVNNVLYQGQCEACACYNHSNTCDSL--NG----ECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPR----LDYQMTELCSSYSSENY-------VCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLG------STSENC-DKLTGECDCRTNFVNRQCDSCTSGL-YLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDR-CEKCVEGYFNYPN-----CKKCLC 622 HSP 3 Score: 63.5438 bits (153), Expect = 3.956e-9 Identity = 68/266 (25.56%), Postives = 96/266 (36.09%), Query Frame = 2
Query: 947 KCICNNHADKCDKVIENGISVAKCNCKHNTCGSNCETC-CPLFNQRPWKPVAVCE----------------ECNC-----NRKSNECS---YNQTVATLQLSVNKMGRMEGGGVCIN------CQENTEGINCEKCIKGFYRPWNVLATELYP-CTPCQCHGLGTIGSCVSNGEDIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGECLPCPCSIDGSVRTSFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCY 1648
+C CNN+ + CD V +C C +NT G+ C+ C + K C+ EC+C NR+ + C+ Y + + + GR+ C C E+ G CEKC++G++ YP C C C C D G C C CD C + +C+PC C GS+ C C +V D C C GYF LD + C PCY
Sbjct: 478 RCKCNNNQESCDPV------TGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFN---------YPNCKKCLCSPHALSADC--------DLNNGKCKCGSNVINRICDKCENLHYWNNRTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRD-CSKCDVGYFGLDVTGCKKCPPCY 718 The following BLAST results are available for this feature:
BLAST of Laminin subunit alpha-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Laminin subunit alpha-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Laminin subunit alpha-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Laminin subunit alpha-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Laminin subunit alpha-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Laminin subunit alpha-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5 |
