Glucosylceramidase 4
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009768 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Glucosylceramidase 4 vs. TrEMBL
Match: A0A158P527 (DDE_3 domain-containing protein OS=Tetranychus urticae OX=32264 PE=4 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 4.870e-8 Identity = 36/119 (30.25%), Postives = 57/119 (47.90%), Query Frame = 3 Query: 561 MWL*VSENRIGDHVFLEIPNSVNAQFYQEHYLRRGLISFINKHHSEYDVLFWLDPVSAHYANNTPSVLRFFLLRKSTILQTSTN----GAIENFWSFLKSKVYQAGWGLSQFPNKRCKL 905 +W+ +S+ F+ ++NA Y +R+ L FI K++ E +FW D S HYA T L ++ Q N IE FW+ LK KVY+ GW S +P+ + ++ Sbjct: 196 VWIAISKKGHSQPFFIPAKCALNADSYSSECIRKRLFPFIQKNYQEKRYIFWPDGASCHYARTTIDTLNELGIKFIEKEQNPPNIPQLRPIERFWAHLKQKVYEGGWSASSYPSLKARI 314
BLAST of Glucosylceramidase 4 vs. TrEMBL
Match: T2MJF3 (WD repeat-containing protein 64 (Fragment) OS=Hydra vulgaris OX=6087 GN=WDR64 PE=2 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 6.221e-6 Identity = 41/142 (28.87%), Postives = 65/142 (45.77%), Query Frame = 3 Query: 558 MMWL*VSENRIGDHVFLEIPNSVNAQFYQEHYLRRGLISFINKHHSEYDVLFWLDPVSAHYANNTPSVLRF----FLLRKSTILQTSTNGAIENFWSFLKSKVYQAG----WGL----------SQF-PNKRCKL*KYCKTC 926 ++WL S + F+ +VN Y+ + +R+ L+ FIN +H++ + +FW D S+HYA S F+ + IE+FW+ LK KVY++ +GL QF P RC+ KTC Sbjct: 167 LVWLAASNKGVSKPFFIPSGLAVNKAIYEMNCIRKRLVPFINAYHADGNYIFWPDLASSHYAKTVTSCYETHNINFIKKVDNPPAVPECRPIEDFWAILKGKVYESAPRSPYGLITEHRGRAFNRQFTPPNRCR-----KTC 303
BLAST of Glucosylceramidase 4 vs. TrEMBL
Match: A0A443RZ87 (Uncharacterized protein OS=Leptotrombidium deliense OX=299467 GN=B4U80_05270 PE=4 SV=1) HSP 1 Score: 58.5362 bits (140), Expect = 6.446e-6 Identity = 33/112 (29.46%), Postives = 55/112 (49.11%), Query Frame = 3 Query: 546 PQE*MMWL*VSENRIGDHVFLEIPNSVNAQFYQEHYLRRGLISFINKHHSEYDVLFWLDPVSAHYANNTPSVLRF----FLLRKSTILQTSTNGAIENFWSFLKSKVYQAGW 869 P++ ++WL +S N I F++ ++N Q Y ++ + R L FI K H +FW D S+HY + T L+ ++ + + IE FW+ LK VY G+ Sbjct: 188 PEKVLVWLAISRNGISKPYFMKSKGAMNMQIYSKYCISRRLSKFIKKLHYNDKFIFWPDNASSHYGSVTLKTLKKLNIPYIPKSHNPVNVPQLRPIERFWAILKRNVYANGY 299
BLAST of Glucosylceramidase 4 vs. Planmine SMEST
Match: SMESG000081253.1 (SMESG000081253.1) HSP 1 Score: 83.9593 bits (206), Expect = 3.938e-20 Identity = 39/40 (97.50%), Postives = 39/40 (97.50%), Query Frame = 3 Query: 42 MKPGSVGHSELELVDGLTTDFVYPSDKTREISTAVPLGHA 161 MKPGSVGHSELELVDGLTTD VYPSDKTREISTAVPLGHA Sbjct: 1 MKPGSVGHSELELVDGLTTDLVYPSDKTREISTAVPLGHA 40 The following BLAST results are available for this feature:
BLAST of Glucosylceramidase 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Glucosylceramidase 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of Glucosylceramidase 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Glucosylceramidase 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009768 ID=SMED30009768|Name=Glucosylceramidase 4|organism=Schmidtea mediterranea sexual|type=transcript|length=991bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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