Myosin regulatory light chain

Overview


Name	Myosin regulatory light chain
Smed ID	SMED30009749
Length (bp)	1350

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Myosin regulatory light chain (SMED30009749) t-SNE clustered cells

Violin plots show distribution of expression levels for Myosin regulatory light chain (SMED30009749) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Myosin regulatory light chain (SMED30009749) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Myosin regulatory light chain (SMED30009749) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30009749

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
anterior pole progenitor cell	SMED30009749	h1SMcG0004594	SMED30009749	smed_20140614	PMID:29906446 Zeng et al., 2018
nervous system	SMED30009749	h1SMcG0004594	dd_Smed_v4_445_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30009749	h1SMcG0004594	dd_Smed_v4_445_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30009749	h1SMcG0004594	dd_Smed_v4_445_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30009749	h1SMcG0004594	dd_Smed_v4_445_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30009749	h1SMcG0004594	dd_Smed_v4_445_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30009749	h1SMcG0004594	Contig42496	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30009749	h1SMcG0004594	Contig42496	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
pharynx musculature	SMED30009749	h1SMcG0004594	dd_Smed_v6_445_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
musculature system	SMED30009749	h1SMcG0004594	dd_Smed_v6_445_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Myosin regulatory light chain vs. Ensembl Human
Match: MYL9 (myosin light chain 9 [Source:HGNC Symbol;Acc:HGNC:15754])

HSP 1 Score: 194.512 bits (493), Expect = 1.009e-59
Identity = 91/154 (59.09%), Postives = 119/154 (77.27%), Query Frame = 1

Query:  277 QRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDD 735
            QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D+ L+ M+SE+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  GF+ E+++++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D
Sbjct:   16 QRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKD 169

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BLAST of Myosin regulatory light chain vs. Ensembl Human
Match: MYL12B (myosin light chain 12B [Source:HGNC Symbol;Acc:HGNC:29827])

HSP 1 Score: 192.586 bits (488), Expect = 4.467e-59
Identity = 91/157 (57.96%), Postives = 118/157 (75.16%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

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Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

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Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

[Close]

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-4 (Myosin regulatory light chain [Source:UniProtKB/Swiss-Prot;Acc:Q09510])

HSP 1 Score: 196.823 bits (499), Expect = 4.775e-61
Identity = 93/168 (55.36%), Postives = 127/168 (75.60%), Query Frame = 1

Query:  238 MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPG--PLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDD 735
            MA   + +  +R QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDLKDM+ASLG+  +++ +  M++E+PG  P+NFTMF+ LFGEKL+G+DPEE +RNAF+ FD DN G L EE++++LL  MG+ ++ E++ + +++AP+ GG+ DY  F R +K G  D
Sbjct:    1 MASRKTVNRRQRPQRATSNVFAMFDQAQIQEFKEAFNMIDQNRDGFIDQEDLKDMFASLGKEVTEQFIDSMINEAPGAQPINFTMFLTLFGEKLTGTDPEEVIRNAFQCFDEDNSGKLNEEHLRELLTTMGERYSEEQVDELFRDAPIKGGQFDYVEFTRMLKHGTKD 168

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-2 (Myosin regulatory light chain 2 [Source:UniProtKB/Swiss-Prot;Acc:P19626])

HSP 1 Score: 138.272 bits (347), Expect = 1.087e-38
Identity = 66/146 (45.21%), Postives = 100/146 (68.49%), Query Frame = 1

Query:  307 FPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLEN-MGDNFTAEEIRQTWK-EAPLTGGKLDYEAFVRQIKRGKDDE 738
            F Q  IQEFKEAF ++DQN+DG ID  DLKD+YAS+G+   D ++  M+ E+ GP+NFT+F+ LFGE+L+G+DPE ++  AF MFD  + G + E+ +  +L+N  G+    +E++  +K + P+ GG++DY+AF   I  G  DE
Sbjct:   21 FDQKTIQEFKEAFGIMDQNKDGIIDKSDLKDLYASMGQIAPDSQIDAMIKEASGPINFTVFLTLFGERLTGTDPEATIVGAFAMFDKKDCGKIKEDDLIKILQNKRGEPLDEDEVKAMYKGKPPIEGGEVDYKAFAHLITTGAQDE 166

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-1 (Myosin regulatory light chain 1 [Source:UniProtKB/Swiss-Prot;Acc:P19625])

HSP 1 Score: 137.887 bits (346), Expect = 1.425e-38
Identity = 66/146 (45.21%), Postives = 100/146 (68.49%), Query Frame = 1

Query:  307 FPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLEN-MGDNFTAEEIRQTWK-EAPLTGGKLDYEAFVRQIKRGKDDE 738
            F Q  IQEFKEAF ++DQN+DG ID  DLKD+YAS+G+   D ++  M+ E+ GP+NFT+F+ LFGE+L+G+DPE ++  AF MFD  + G + E+ +  +L+N  G+    +E++  +K + P+ GG++DY+AF   I  G  DE
Sbjct:   21 FDQKTIQEFKEAFGIMDQNKDGIIDKSDLKDLYASMGQIAPDSQIDAMIKEASGPINFTVFLTLFGERLTGTDPEATIIGAFAMFDKKDCGKIKEDDLIKILQNKRGEPLDEDEVKAMYKGKPPIEGGEVDYKAFAHLITTGAQDE 166

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 101.679 bits (252), Expect = 1.808e-25
Identity = 50/139 (35.97%), Postives = 88/139 (63.31%), Query Frame = 1

Query:  307 FPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSE----SPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFV 708
              + QI EFKEAF++ D++ DG I  ++L  +  SLG+ P++ ELQ+M++E      G ++F  F+ +   K+  +D EE +R AF++FD D  GF++   ++ ++ N+G+  T EE+ +  +EA + G G+++YE FV
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFV 143

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 78.9518 bits (193), Expect = 1.145e-17
Identity = 37/105 (35.24%), Postives = 67/105 (63.81%), Query Frame = 1

Query:  409 SLGRTPSDKELQEMLSE----SPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFV 708
            SLG+ P++ ELQ+M++E      G ++F  F+ +   K+  +D EE +R AF++FD D  GF++   ++ ++ N+G+  T EE+ +  +EA + G G+++YE FV
Sbjct:    3 SLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFV 107

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BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339504)

HSP 1 Score: 193.356 bits (490), Expect = 1.380e-59
Identity = 90/157 (57.32%), Postives = 117/157 (74.52%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            KRAQRATSNVF MF Q QI EFKEAF MIDQNRDGF++ EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ LFGE+L G+DPE+ ++NAF  FD +N G L E+ +++LL  MGD FT E++ + ++EAP+  G  DY  F R +K G  D+
Sbjct:   15 KRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDK 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339503)

HSP 1 Score: 193.356 bits (490), Expect = 1.380e-59
Identity = 90/157 (57.32%), Postives = 117/157 (74.52%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            KRAQRATSNVF MF Q QI EFKEAF MIDQNRDGF++ EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ LFGE+L G+DPE+ ++NAF  FD +N G L E+ +++LL  MGD FT E++ + ++EAP+  G  DY  F R +K G  D+
Sbjct:   15 KRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDK 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0070877)

HSP 1 Score: 193.356 bits (490), Expect = 1.380e-59
Identity = 90/157 (57.32%), Postives = 117/157 (74.52%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            KRAQRATSNVF MF Q QI EFKEAF MIDQNRDGF++ EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ LFGE+L G+DPE+ ++NAF  FD +N G L E+ +++LL  MGD FT E++ + ++EAP+  G  DY  F R +K G  D+
Sbjct:   15 KRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDK 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339502)

HSP 1 Score: 193.356 bits (490), Expect = 1.380e-59
Identity = 90/157 (57.32%), Postives = 117/157 (74.52%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            KRAQRATSNVF MF Q QI EFKEAF MIDQNRDGF++ EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ LFGE+L G+DPE+ ++NAF  FD +N G L E+ +++LL  MGD FT E++ + ++EAP+  G  DY  F R +K G  D+
Sbjct:   15 KRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDK 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0345294)

HSP 1 Score: 193.356 bits (490), Expect = 1.380e-59
Identity = 90/157 (57.32%), Postives = 117/157 (74.52%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            KRAQRATSNVF MF Q QI EFKEAF MIDQNRDGF++ EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ LFGE+L G+DPE+ ++NAF  FD +N G L E+ +++LL  MGD FT E++ + ++EAP+  G  DY  F R +K G  D+
Sbjct:   15 KRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDK 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl9a (myosin, light chain 9a, regulatory [Source:ZFIN;Acc:ZDB-GENE-041010-120])

HSP 1 Score: 201.445 bits (511), Expect = 1.495e-62
Identity = 95/158 (60.13%), Postives = 122/158 (77.22%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PSD+ L+ M+SE+PGP+NFTMF+ +FGE+L+G+DPE+ +RNAF  FD D  GF+ E++++DLL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDK 171

[Close]

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PSD+ L+ M+SE+PGP+NFTMF+ +FGE+L+G+DPE+ +RNAF  FD D  GF+ E++++DLL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDK 171

[Close]

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PSD+ L+ M+SE+PGP+NFTMF+ +FGE+L+G+DPE+ +RNAF  FD D  GF+ E++++DLL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDK 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl12.1 (myosin, light chain 12, genome duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030131-4918])

HSP 1 Score: 197.593 bits (501), Expect = 4.187e-61
Identity = 93/158 (58.86%), Postives = 120/158 (75.95%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L+ M++E+PGP+NFTMF+ +FGEKL+G+DPEE +RNAF  FD +  GF+ E+Y+++LL  MGD FT EE+ + ++EAP+      +Y  F R +K G  D+
Sbjct:   13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPADDYLEAMMTEAPGPINFTMFLTMFGEKLNGTDPEEVIRNAFACFDEEGTGFIHEDYLRELLTTMGDRFTDEEVDELFREAPIDKKSNFNYVEFTRILKHGAKDK 170

[Close]

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L+ M++E+PGP+NFTMF+ +FGEKL+G+DPEE +RNAF  FD +  GF+ E+Y+++LL  MGD FT EE+ + ++EAP+      +Y  F R +K G  D+
Sbjct:   13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPADDYLEAMMTEAPGPINFTMFLTMFGEKLNGTDPEEVIRNAFACFDEEGTGFIHEDYLRELLTTMGDRFTDEEVDELFREAPIDKKSNFNYVEFTRILKHGAKDK 170

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: myl9 (myosin light chain 9 [Source:NCBI gene;Acc:100124811])

HSP 1 Score: 194.512 bits (493), Expect = 9.449e-60
Identity = 93/159 (58.49%), Postives = 122/159 (76.73%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRG---KDD 735
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM AS+G+ P+D+ L+ M+SE+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  GF+ E+++++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G   KDD
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFINEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKEKDD 172

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: myl12b (myosin light chain 12B [Source:NCBI gene;Acc:448060])

HSP 1 Score: 188.734 bits (478), Expect = 1.350e-57
Identity = 90/157 (57.32%), Postives = 118/157 (75.16%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D+ L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+ +++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   15 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGIGSIQEDTLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYNEFTRILKHGAKDK 171

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: ap5z1 (adaptor related protein complex 5 subunit zeta 1 [Source:Xenbase;Acc:XB-GENE-6034870])

HSP 1 Score: 175.252 bits (443), Expect = 1.866e-52
Identity = 86/159 (54.09%), Postives = 116/159 (72.96%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGR-TPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKE-APLTGGKLDYEAFVRQIKRGKDDE 738
            KRA+ A SNVF MF Q QIQEFKEAFT++DQNRDGFID EDL+D +A+LGR    ++EL+EML E+PGP+NFT+F+ +FGEKL G+DPEE++ NAFK+FD +  G L  EYI+++L    + FT+EE+ Q +    P   G L+Y+  V  I  G++ E
Sbjct:    8 KRAEGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKEDLRDTFAALGRLNVKNEELEEMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGLLKSEYIREMLMTQAERFTSEEVDQMFTAFPPDVTGNLNYKNLVHIITHGEEKE 166

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: ap5z1 (adaptor related protein complex 5 subunit zeta 1 [Source:Xenbase;Acc:XB-GENE-6034870])

HSP 1 Score: 176.407 bits (446), Expect = 6.456e-52
Identity = 87/168 (51.79%), Postives = 118/168 (70.24%), Query Frame = 1

Query:  241 AEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGR-TPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKE-APLTGGKLDYEAFVRQIKRGKDDE 738
            A    +   KRA+ A SNVF MF Q QIQEFKEAFT++DQNRDGFID EDL+D +A+LGR    ++EL+EML E+PGP+NFT+F+ +FGEKL G+DPEE++ NAFK+FD +  G L  EYI+++L    + FT+EE+ Q +    P   G L+Y+  V  I  G++ E
Sbjct:   77 ANTSPKKAKKRAEGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKEDLRDTFAALGRLNVKNEELEEMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGLLKSEYIREMLMTQAERFTSEEVDQMFTAFPPDVTGNLNYKNLVHIITHGEEKE 244

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: myl10 (myosin light chain 10 [Source:NCBI gene;Acc:100170437])

HSP 1 Score: 165.622 bits (418), Expect = 1.080e-48
Identity = 81/152 (53.29%), Postives = 109/152 (71.71%), Query Frame = 1

Query:  283 ATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGR-TPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKE-APLTGGKLDYEAFVRQIKRGKD 732
            A+SNVF MF Q+QIQEFKEAFT++DQNRDGFID  DL+D +A+LGR     +EL +M+ E+PGP+NFT+F+ +FGEKL G+DPEE++ NAFK+FD D KG +  +YI+++L    D FT EEI Q +    P   G LDY+     I  G++
Sbjct:   13 ASSNVFSMFDQSQIQEFKEAFTIMDQNRDGFIDKGDLRDTFAALGRINVKSEELDDMVQEAPGPINFTVFLTMFGEKLKGTDPEETILNAFKIFDPDGKGHIKADYIREMLTTQADRFTQEEITQMFTAFPPDVAGNLDYKNLCYIITHGEE 164

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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl9 (myosin, light polypeptide 9, regulatory [Source:MGI Symbol;Acc:MGI:2138915])

HSP 1 Score: 192.971 bits (489), Expect = 2.249e-59
Identity = 90/154 (58.44%), Postives = 119/154 (77.27%), Query Frame = 1

Query:  277 QRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDD 735
            QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D+ L+ M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  GF+ E+++++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D
Sbjct:   16 QRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKD 169

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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Gm49909 (predicted gene, 49909 [Source:MGI Symbol;Acc:MGI:6270609])

HSP 1 Score: 192.586 bits (488), Expect = 3.067e-59
Identity = 91/157 (57.96%), Postives = 118/157 (75.16%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl12b (myosin, light chain 12B, regulatory [Source:MGI Symbol;Acc:MGI:107494])

HSP 1 Score: 192.586 bits (488), Expect = 3.067e-59
Identity = 91/157 (57.96%), Postives = 118/157 (75.16%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

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Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D  L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl12a (myosin, light chain 12A, regulatory, non-sarcomeric [Source:MGI Symbol;Acc:MGI:1914518])

HSP 1 Score: 191.43 bits (485), Expect = 1.016e-58
Identity = 90/157 (57.32%), Postives = 119/157 (75.80%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM AS+G+ P+D+ L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEAIGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|P08051|MLR_SPISA (Myosin regulatory light chain, smooth muscle OS=Spisula sachalinensis OX=81899 PE=1 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 1.914e-69
Identity = 103/160 (64.38%), Postives = 130/160 (81.25%), Query Frame = 1

Query:  259 SGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            S  K+A+ ATS+V   F QNQIQE KEAFTMIDQNRDG ID+ DLK+MY++LG  P D  LQ M+ E+P  +NFT F++LF EK+SG+DPEE+LRNAF+MFDSDN G++ EEY+KDLLENMGDNF+ +E+RQTWKEAP+ GGK+DY AFV +IK  + D+
Sbjct:    1 SDDKKAKAATSSVLTKFTQNQIQEMKEAFTMIDQNRDGLIDVSDLKEMYSNLGTAPQDSVLQAMVKEAP-QMNFTGFLSLFSEKMSGTDPEETLRNAFQMFDSDNTGYIPEEYMKDLLENMGDNFSKDEVRQTWKEAPIAGGKVDYNAFVSKIKGKEQDD 159

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|P04113|MLRA_MIZYE (Myosin regulatory light chain A, smooth adductor muscle OS=Mizuhopecten yessoensis OX=6573 PE=1 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 3.949e-68
Identity = 106/156 (67.95%), Postives = 124/156 (79.49%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            RAQRATSNVF   PQ  +QE KEAFTMIDQNRDGFIDI DLK+M++SLGRTP DKEL  ML E+PGPLNFTMF+++F +KLSG+D EE+LRNAF MFD  +   L  EYIKDLLENMGDNFT +E+R T+KEAP+TGGK DY  F   IK   ++E
Sbjct:    5 RAQRATSNVFARLPQKLMQEMKEAFTMIDQNRDGFIDINDLKEMFSSLGRTPDDKELTAMLKEAPGPLNFTMFLSIFSDKLSGTDTEETLRNAFAMFDELDTKKLNIEYIKDLLENMGDNFTKDEMRMTFKEAPVTGGKFDYVKFTAMIKGSGEEE 160

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|P04112|MLRB_MIZYE (Myosin regulatory light chain B, smooth adductor muscle OS=Mizuhopecten yessoensis OX=6573 PE=1 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 8.247e-65
Identity = 102/155 (65.81%), Postives = 120/155 (77.42%), Query Frame = 1

Query:  274 AQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            A +A S V    PQ QIQE KEAFTMIDQNRDGFIDI DLK+M++SLGRTP DKEL  ML E+PGPLNFTMF+++F +KLSG+D EE+LRNAF MFD  +   L  EYIKDLLENMGDNFT +E+R T+KEAP+TGGK DY  F   IK   ++E
Sbjct:    1 ADKAASGVLTKLPQKQIQEMKEAFTMIDQNRDGFIDINDLKEMFSSLGRTPDDKELTAMLKEAPGPLNFTMFLSIFSDKLSGTDTEETLRNAFAMFDELDTKKLNIEYIKDLLENMGDNFTKDEMRMTFKEAPVTGGKFDYVKFTAMIKGSGEEE 155

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|Q09510|MLRH_CAEEL (Myosin regulatory light chain OS=Caenorhabditis elegans OX=6239 GN=mlc-4 PE=1 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 6.074e-60
Identity = 93/168 (55.36%), Postives = 127/168 (75.60%), Query Frame = 1

Query:  238 MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPG--PLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDD 735
            MA   + +  +R QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDLKDM+ASLG+  +++ +  M++E+PG  P+NFTMF+ LFGEKL+G+DPEE +RNAF+ FD DN G L EE++++LL  MG+ ++ E++ + +++AP+ GG+ DY  F R +K G  D
Sbjct:    1 MASRKTVNRRQRPQRATSNVFAMFDQAQIQEFKEAFNMIDQNRDGFIDQEDLKDMFASLGKEVTEQFIDSMINEAPGAQPINFTMFLTLFGEKLTGTDPEEVIRNAFQCFDEDNSGKLNEEHLRELLTTMGERYSEEQVDELFRDAPIKGGQFDYVEFTRMLKHGTKD 168

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|P24032|MLRN_CHICK (Myosin regulatory light chain 2, smooth muscle minor isoform OS=Gallus gallus OX=9031 PE=2 SV=2)

HSP 1 Score: 195.667 bits (496), Expect = 1.610e-59
Identity = 92/157 (58.60%), Postives = 120/157 (76.43%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D+ L  M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  GF+ E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGFIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170

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BLAST of Myosin regulatory light chain vs. TrEMBL
Match: Q5DB55 (Myosin regulatory light chain 2, smooth muscle minor isoform (G1) OS=Schistosoma japonicum OX=6182 GN=EWB00_004284 PE=2 SV=1)

HSP 1 Score: 280.026 bits (715), Expect = 1.541e-89
Identity = 134/161 (83.23%), Postives = 147/161 (91.30%), Query Frame = 1

Query:  253 SQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDD 735
            S  GGKRAQRATSNVFGMFPQNQIQEFKEAF++IDQNRDGFIDIEDLKDMYASLGRTP+DKEL+EML ESPGPLNFTMFINLFGEKL+G+DPE++LRNAF MFD  NK +L EEYIKDLL+NMGDNF+AEEIRQTWKEAP+  GKLDY+AFV  IKRG  D
Sbjct:   37 SGGGGKRAQRATSNVFGMFPQNQIQEFKEAFSLIDQNRDGFIDIEDLKDMYASLGRTPTDKELKEMLDESPGPLNFTMFINLFGEKLNGTDPEDALRNAFAMFDPGNKRYLEEEYIKDLLQNMGDNFSAEEIRQTWKEAPIKEGKLDYDAFVNLIKRGNQD 197

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BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A183AZT2 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS12467 PE=4 SV=1)

HSP 1 Score: 280.411 bits (716), Expect = 2.806e-89
Identity = 135/163 (82.82%), Postives = 146/163 (89.57%), Query Frame = 1

Query:  247 EGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDD 735
            E S  GGKRAQRATSNVFGMFPQNQIQEFKEAFT+IDQNRDGFIDIEDLKDMYASLGRTPSD EL+EML ESPGPLNFTMFINLFGEKL+G+DPE++LRNAF MFD  NK +L EEYIKDLL+NMGDNF+AEEIRQTWKEAP+  GKLDY+ FV  IKRG  D
Sbjct:   58 ESSSGGGKRAQRATSNVFGMFPQNQIQEFKEAFTLIDQNRDGFIDIEDLKDMYASLGRTPSDAELKEMLDESPGPLNFTMFINLFGEKLNGTDPEDALRNAFAMFDPGNKRYLEEEYIKDLLQNMGDNFSAEEIRQTWKEAPIKEGKLDYDQFVTLIKRGNQD 220

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BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A1S8WJA4 (EF hand OS=Opisthorchis viverrini OX=6198 GN=X801_09678 PE=4 SV=1)

HSP 1 Score: 286.574 bits (732), Expect = 4.067e-89
Identity = 139/175 (79.43%), Postives = 152/175 (86.86%), Query Frame = 1

Query:  220 SLFLLKMAEEGSQSGG--KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            SL  +  AEE  +SGG  KRAQRATSNVFGMFPQNQIQEFKEAFT+IDQNRDGFIDIEDLKDMYASLGRTPSD EL+EML ESPGPLNFTMFINLFGEKL+G+DPE++LRNAF MFD  NK +L EEYIKDLL+NMGDNF+AEEIRQTWKEAP+  GKLDY  FV  IKRG  D+
Sbjct:  228 SLLCVTPAEEKKESGGGGKRAQRATSNVFGMFPQNQIQEFKEAFTLIDQNRDGFIDIEDLKDMYASLGRTPSDAELKEMLDESPGPLNFTMFINLFGEKLNGTDPEDALRNAFAMFDPGNKRYLEEEYIKDLLQNMGDNFSAEEIRQTWKEAPIKEGKLDYNEFVTLIKRGNQDQ 402

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BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A4V6RH67 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001843 PE=4 SV=1)

HSP 1 Score: 283.108 bits (723), Expect = 5.043e-89
Identity = 137/168 (81.55%), Postives = 149/168 (88.69%), Query Frame = 1

Query:  241 AEEGSQSGG--KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            AEE  +SGG  KRAQRATSNVFGMFPQNQIQEFKEAFT+IDQNRDGFIDIEDLKDMYASLGRTPSD EL+EML ESPGPLNFTMFINLFGEKL+G+DPE++LRNAF MFD  NK +L EEYIKDLL+NMGDNF+AEEIRQTWKEAP+  GKLDY  FV  IKRG  D+
Sbjct:  146 AEEKKESGGGGKRAQRATSNVFGMFPQNQIQEFKEAFTLIDQNRDGFIDIEDLKDMYASLGRTPSDAELKEMLDESPGPLNFTMFINLFGEKLNGTDPEDALRNAFAMFDPSNKRYLEEEYIKDLLQNMGDNFSAEEIRQTWKEAPIKEGKLDYNEFVTLIKRGNQDQ 313

[Close]

BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A183MFP9 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS14874 PE=4 SV=1)

HSP 1 Score: 277.715 bits (709), Expect = 1.170e-88
Identity = 133/156 (85.26%), Postives = 144/156 (92.31%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDD 735
            KRAQRATSNVFGMFPQNQIQEFKEAFT+IDQNRDGFIDIEDLKDMYASLGRTP+DKEL+EML ESPGPLNFTMFINLFGEKL+G+DPE++LRNAF MFD  NK +L EEYIKDLLENMGDNF+AEEIRQTWKEAP+  GKLDY+AFV  IKRG  D
Sbjct:   41 KRAQRATSNVFGMFPQNQIQEFKEAFTLIDQNRDGFIDIEDLKDMYASLGRTPTDKELKEMLDESPGPLNFTMFINLFGEKLNGTDPEDALRNAFAMFDPGNKRYLEEEYIKDLLENMGDNFSAEEIRQTWKEAPIKEGKLDYDAFVNLIKRGNQD 196

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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000048105.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:9819022:9829334:-1 gene:ENSAMXG00000037886.1 transcript:ENSAMXT00000048105.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 201.445 bits (511), Expect = 2.170e-62
Identity = 97/187 (51.87%), Postives = 132/187 (70.59%), Query Frame = 1

Query:  181 IFCIISKTFSFRHSLFLLKMAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            ++C  S    F+     +       ++  KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D+ L+ M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  GF+ E+Y+++LL  MGD FT EE+ + ++EAP+   G  +Y AF R +K G  D+
Sbjct:    3 LYCCFS-PLEFKGITAKMSSKRAKGKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYVAFTRILKHGAKDK 188

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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: myl9a (myosin regulatory light polypeptide 9-like [Source:NCBI gene;Acc:103022187])

HSP 1 Score: 199.904 bits (507), Expect = 3.741e-62
Identity = 94/158 (59.49%), Postives = 122/158 (77.22%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PSD+ L+ M+SE+PGP+NFTMF+ +FGE+L+G+DPE+ +RNAF  FD D  GF+ E+++++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   14 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDGSGFIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYTEFTRILKHGAKDK 171

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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000046001.1 (myosin regulatory light chain 2, smooth muscle minor isoform-like [Source:NCBI gene;Acc:103039566])

HSP 1 Score: 195.667 bits (496), Expect = 1.750e-60
Identity = 91/162 (56.17%), Postives = 122/162 (75.31%), Query Frame = 1

Query:  256 QSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            ++  KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGF+D EDL DM ASLG+ P+D  L+ M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  GF+ E+Y+++LL  MGD FT EE+ + ++EAP+      +Y  F R +K G  D+
Sbjct:    9 KTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFVDKEDLHDMLASLGKNPTDDYLEAMMTEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKSNFNYVEFTRILKHGAKDK 170

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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: myl9b (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:103045271])

HSP 1 Score: 195.282 bits (495), Expect = 2.686e-60
Identity = 92/158 (58.23%), Postives = 120/158 (75.95%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PSD  L+ M+SE+PGP+NFTMF+ +FGE+L+G+DPE+ +RNAF  FD +  G + E+++++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G  D+
Sbjct:   13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDDYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDK 170

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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000051744.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000067.1:2135412:2141173:-1 gene:ENSAMXG00000034506.1 transcript:ENSAMXT00000051744.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 179.874 bits (455), Expect = 1.216e-54
Identity = 87/155 (56.13%), Postives = 116/155 (74.84%), Query Frame = 1

Query:  280 RATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGR-TPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            RA+SNVF MF Q QIQEFKEAFT+IDQNRDGFID EDLKD YASLG+    D EL++ML E+ GP+NFTMF+NLFGEKL G+DPE+++ NAFK+FD + KG++  E +K +L    + F+AEE+RQ ++ + + + G LDY +    I  G++ E
Sbjct:    1 RASSNVFSMFEQTQIQEFKEAFTLIDQNRDGFIDKEDLKDTYASLGKLNVKDNELEDMLKEASGPINFTMFLNLFGEKLHGTDPEDTILNAFKLFDPEAKGYIHTEELKHVLMTQANKFSAEEVRQMFQSSNIDSAGNLDYRSLCYIITHGEEQE 155

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BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: myl9a (myosin, light chain 9a, regulatory [Source:ZFIN;Acc:ZDB-GENE-041010-120])

HSP 1 Score: 166.777 bits (421), Expect = 5.850e-50
Identity = 88/167 (52.69%), Postives = 121/167 (72.46%), Query Frame = 1

Query:  247 EGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRG---KDD 735
            +G  S  KR QRATSNVF MF ++QIQEFKEAF ++DQNRDG ID EDL+DM ASLG+TP+++ L+EM+ E+ GP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD D  G + E++ ++L+  MGD F+  ++ Q    AP+   G+L+Y  F   +K G   KDD
Sbjct:   11 KGKSSFRKRPQRATSNVFAMFDKSQIQEFKEAFNIMDQNRDGLIDREDLQDMLASLGKTPTEEMLEEMMGEASGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEDGTGRINEDHFRELMTTMGDRFSDAQVDQLLAAAPIDQRGELNYLEFTHILKHGDREKDD 177

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BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006008.1 (pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-1 gene:ENSPMAG00000005430.1 transcript:ENSPMAT00000006008.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 102.834 bits (255), Expect = 2.411e-26
Identity = 49/142 (34.51%), Postives = 91/142 (64.08%), Query Frame = 1

Query:  307 FPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSE----SPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFVRQI 717
              + QI EFKEAF++ D++ DG I +++L  +  SLG+ P++ ELQ+M++E      G ++F  F+ +   K+  +D EE +R AF++FD D  G+++   ++ ++ N+G+  T EE+ +  +EA + G G+++YE FV+ +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITVKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146

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BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 101.293 bits (251), Expect = 8.837e-26
Identity = 49/142 (34.51%), Postives = 90/142 (63.38%), Query Frame = 1

Query:  307 FPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSE----SPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFVRQI 717
              + QI EFKEAF++ D++ DG I  ++L  +  SLG+ P++ ELQ+M++E      G ++F  F+ +   K+  +D EE +R AF++FD D  G+++   ++ ++ N+G+  T EE+ +  +EA + G G+++YE FV+ +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146

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BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452])

HSP 1 Score: 101.293 bits (251), Expect = 9.200e-26
Identity = 49/142 (34.51%), Postives = 90/142 (63.38%), Query Frame = 1

Query:  307 FPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSE----SPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFVRQI 717
              + QI EFKEAF++ D++ DG I  ++L  +  SLG+ P++ ELQ+M++E      G ++F  F+ +   K+  +D EE +R AF++FD D  G+++   ++ ++ N+G+  T EE+ +  +EA + G G+++YE FV+ +
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146

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BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CETN1 (centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866])

HSP 1 Score: 82.4185 bits (202), Expect = 1.194e-18
Identity = 50/159 (31.45%), Postives = 89/159 (55.97%), Query Frame = 1

Query:  262 GGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSE----SPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFVRQIKR 723
            G   AQR  S       + Q QE KEAF + D +  G ID+++LK    +LG  P  +E+++M+++      G +NF  F+ +  +K+S  D  E +  AF++FD D  G ++ + +K + + +G+  T EE+++   EA   G G+++ E F+R +K+
Sbjct:   21 GAGGAQRKKSGPKPELSEEQKQEIKEAFDLFDADGSGTIDVKELKVAMRALGFEPKKEEIKKMIADFDKDGSGKINFNDFLAMMTQKMSEKDSREEILKAFRLFDDDETGKISFKNLKRVAKELGETLTDEELQEMIDEADRDGDGEINEEEFLRVMKK 179

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BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 75.485 bits (184), Expect = 8.199e-17
Identity = 40/143 (27.97%), Postives = 77/143 (53.85%), Query Frame = 1

Query:  307 FPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSE----SPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKR 723
              + QI EFKEAF + D++ +G I   +L  +  SLG +PS+ E+ ++++E        + F+ F+ L   +L  +D E+ L  AFK+FD +  G ++   +K +L ++G+  T  E+    +E     G+++ + F   + +
Sbjct:    5 LTEEQIAEFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTSIGEKLTDAEVDDMLREVSDGSGEINIQQFAALLSK 147

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BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Match: CDC31 (Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation [Source:SGD;Acc:S000005783])

HSP 1 Score: 68.5514 bits (166), Expect = 2.780e-14
Identity = 41/137 (29.93%), Postives = 77/137 (56.20%), Query Frame = 1

Query:  313 QNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEML----SESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFV 708
            + Q QE  EAF++ D N DGF+D  +LK    +LG     +E+ +++    SE    + +  F  + GEK+   DP + ++ AF++FD D+ G ++ + ++ + + +G+  T EE+R   +E  L G G+++   F+
Sbjct:   19 EEQKQEIYEAFSLFDMNNDGFLDYHELKVAMKALGFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEKILKRDPLDEIKRAFQLFDDDHTGKISIKNLRRVAKELGETLTDEELRAMIEEFDLDGDGEINENEFI 155

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BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Match: MLC2 (Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring [Source:SGD;Acc:S000006392])

HSP 1 Score: 52.7582 bits (125), Expect = 8.836e-9
Identity = 30/100 (30.00%), Postives = 54/100 (54.00%), Query Frame = 1

Query:  280 RATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGP--------LNFTMFINLFGEKLSGSDPEESLRNAFK 555
             + S  F    Q+ I + K+AF M+D++ DG I   DL  +YA+LG+T +D+E  +M+ ++           ++F +F+++ G+ LS     E L  + K
Sbjct:    3 HSESLTFNQLTQDYINKLKDAFQMLDEDEDGLISRGDLTKIYATLGKTLTDEEWSKMVPDNDTSTAEVGEEGVSFPIFLSIMGKNLSQFPEREELEESLK 102

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BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO44829 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUY1])

HSP 1 Score: 190.66 bits (483), Expect = 4.973e-59
Identity = 92/157 (58.60%), Postives = 117/157 (74.52%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFVRQIKRGKDDE 738
            RA RATSNVF MF Q+QIQEFKEAF MIDQN DGFID EDL DM ASLG+ P+D  L+EM+ ESPGP+NFTMF+ LFGEKL+G+DPE+ +RNAF  FD D  G + E+ +K+ + +MGD F+ E++    ++AP+   G LDY  F R +K GK D+
Sbjct:   13 RAHRATSNVFAMFDQSQIQEFKEAFNMIDQNHDGFIDKEDLHDMLASLGKDPTDGYLEEMVKESPGPINFTMFLTLFGEKLNGTDPEDVIRNAFACFDEDGNGRIHEDLLKEAMMSMGDRFSDEQVDDILRDAPIDKEGFLDYSKFTRILKHGKKDD 169

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO44767 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUX1])

HSP 1 Score: 179.874 bits (455), Expect = 9.143e-55
Identity = 90/160 (56.25%), Postives = 120/160 (75.00%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGP-LNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGK--DDE 738
            RAQRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDLKDMYASLG++P+D+ L +M+ E+ G  +NFTMF+ LFGEKL+G+DPE+ ++NAF  FD +  G + E+ + D+L ++GD F+ +EI +   + P+   G +DY  + + +KRGK  DDE
Sbjct:   13 RAQRATSNVFAMFDQTQIQEFKEAFNMIDQNRDGFIDKEDLKDMYASLGKSPNDQFLDDMIEEASGSTINFTMFLTLFGEKLNGTDPEDVIKNAFGCFDEEGDGRIHEDKLCDMLTSVGDRFSEDEIDEMLHDCPVDKKGYIDYITYTQMLKRGKKEDDE 172

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO25626 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVY3])

HSP 1 Score: 157.918 bits (398), Expect = 5.749e-47
Identity = 76/120 (63.33%), Postives = 97/120 (80.83%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGP-LNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGD 627
            RAQRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDLKDMYASLG++P+D+ L +M+ E+ G  +NFTMF+ LFGEKL+G+DPE+ ++NAF  FD +  G + E+ + D+L ++GD
Sbjct:   13 RAQRATSNVFAMFDQTQIQEFKEAFNMIDQNRDGFIDKEDLKDMYASLGKSPNDQFLDDMIEEASGSTINFTMFLTLFGEKLNGTDPEDVIKNAFGCFDEEGDGRIHEDKLCDMLTSVGD 132

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO42774 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0P5])

HSP 1 Score: 148.288 bits (373), Expect = 4.189e-43
Identity = 77/142 (54.23%), Postives = 101/142 (71.13%), Query Frame = 1

Query:  304 MFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFVRQIKRG 726
            MF Q+QIQEFKEAF MIDQNRDGFID  DLK ++ SLG+  +D+ +++ML+E+ GP+NFTMF+ LFGEK+SG+DPE+ +RNAF  FD + KG + EE +K L  +M D  TAEE      E P T  G++ Y+ F   IK G
Sbjct:    1 MFEQSQIQEFKEAFNMIDQNRDGFIDKNDLKAVFDSLGKLVNDEYVEDMLNEASGPINFTMFLTLFGEKISGTDPEDVIRNAFGSFDLEGKGSIDEEKLKRLCMSMSDRMTAEEWEDMMDECPTTKQGEILYKEFTDIIKNG 142

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO35925 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SK46])

HSP 1 Score: 106.686 bits (265), Expect = 2.801e-27
Identity = 57/161 (35.40%), Postives = 92/161 (57.14%), Query Frame = 1

Query:  259 SGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            S   R  R  SN F    Q+QI+ FKEAF +IDQ+R+G I  +DL+   +SLG+ P+ +E+ +M+   P   NFT F+++FG +++ +DPE+ +  AF   D    G L+ + +++LL  MG      ++ Q + +      G LDY   VR +K G +D+
Sbjct:   14 SQASRVNRVPSNFFTALDQSQIRLFKEAFNIIDQDRNGIITRDDLRGTLSSLGQKPTFEEIDDMMEGKPS-FNFTSFLSMFGLRMANTDPEDIILRAFSCLDDGGDGKLSAKMLRELLTTMGQRMKYSDVTQLFDDVGADQDGNLDYVKLVRLLKNGANDQ 173

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl9a (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:101167032])

HSP 1 Score: 197.593 bits (501), Expect = 5.778e-61
Identity = 95/161 (59.01%), Postives = 123/161 (76.40%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRG---KDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PSD+ L+ M+SE+PGP+NFTMF+ +FGE+L+G+DPE+ +RNAF  FD +  G + E+++++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G   KDDE
Sbjct:   29 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDKDDE 189

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl9a (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:101167032])

HSP 1 Score: 196.823 bits (499), Expect = 5.961e-61
Identity = 95/161 (59.01%), Postives = 123/161 (76.40%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRG---KDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PSD+ L+ M+SE+PGP+NFTMF+ +FGE+L+G+DPE+ +RNAF  FD +  G + E+++++LL  MGD FT EE+ + ++EAP+   G  +Y  F R +K G   KDDE
Sbjct:   13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDKDDE 173

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl12.2 (myosin regulatory light chain 2, smooth muscle minor isoform [Source:NCBI gene;Acc:101165346])

HSP 1 Score: 196.823 bits (499), Expect = 7.543e-61
Identity = 92/158 (58.23%), Postives = 122/158 (77.22%), Query Frame = 1

Query:  268 KRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDDE 738
            KR QRATSNVF MF Q+QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ P+D+ L+ M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  G + EE++++LL  MGD FT E++ + ++EAP+   G  +Y AF R +K G  D+
Sbjct:   13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPVNFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGLIQEEFLRELLTTMGDRFTDEDVDELFREAPIDKKGNFNYVAFTRILKHGAKDK 170

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: ENSORLT00000014284.2 (myosin regulatory light chain 2, smooth muscle minor isoform [Source:NCBI gene;Acc:101159052])

HSP 1 Score: 192.586 bits (488), Expect = 2.902e-59
Identity = 90/156 (57.69%), Postives = 118/156 (75.64%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPL-TGGKLDYEAFVRQIKRGKDD 735
            R QRATSNVF MF Q+QIQEFKEAF MIDQNRDGF+D EDL DM ASLG+ P+D  L+ M++E+PGP+NFTMF+ +FGEKL+G+DPE+ +RNAF  FD +  GF+ E+Y+++LL  MGD FT EE+ + ++EAP+      +Y  F R +K G  D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFVDKEDLHDMLASLGKNPNDDYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTEEEVDELFREAPIDKKSNFNYVEFTRILKHGARD 169

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: MYL2 (myosin regulatory light chain 2, ventricular/cardiac muscle isoform [Source:NCBI gene;Acc:101162219])

HSP 1 Score: 166.007 bits (419), Expect = 4.454e-49
Identity = 80/163 (49.08%), Postives = 114/163 (69.94%), Query Frame = 1

Query:  250 GSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGR-TPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKE-APLTGGKLDYEAFVRQIKRGKD 732
              +   KR++ A+SNVF MF Q QIQEFKEAFT++DQNRDGFID  DL+D +A+LGR     +E+ +ML E+PGP+NFT+F+ +FGEKL G+DPEE++ NAFK+FD +  G L ++++ ++L    D F+AEE+ Q +    P   G LDY+  V  I  G++
Sbjct:    2 APKKAKKRSEGASSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRLNVKQEEIDDMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGTLKKDFVTEMLTTQADRFSAEEMEQMFVAFPPDVAGNLDYKNLVHIITHGEE 164

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000066078.1 (SMESG000066078.1)

HSP 1 Score: 340.502 bits (872), Expect = 2.569e-117
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 1

Query:  238 MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE
Sbjct:    1 MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 167

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000026825.1 (SMESG000026825.1)

HSP 1 Score: 308.916 bits (790), Expect = 6.393e-105
Identity = 147/167 (88.02%), Postives = 160/167 (95.81%), Query Frame = 1

Query:  238 MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            M +E +QSGGKRAQRATSNVFGMFPQ QIQEFKEAFTM+DQNRDGFIDIEDLKDMYASLGRTP+DKEL+EML ESPGPLNFTMFINLFGEKL+G+DPEE+LRNAFKMFDS+NK +L EEYIKDLL+NMGDNFT EEIRQTWKEAPL+GGKLDYEAFVRQIKRGK+DE
Sbjct:    1 MGDEAAQSGGKRAQRATSNVFGMFPQQQIQEFKEAFTMVDQNRDGFIDIEDLKDMYASLGRTPTDKELREMLEESPGPLNFTMFINLFGEKLNGTDPEEALRNAFKMFDSNNKEYLEEEYIKDLLQNMGDNFTPEEIRQTWKEAPLSGGKLDYEAFVRQIKRGKEDE 167

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000000175.1 (SMESG000000175.1)

HSP 1 Score: 182.185 bits (461), Expect = 2.698e-55
Identity = 83/156 (53.21%), Postives = 118/156 (75.64%), Query Frame = 1

Query:  271 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 738
            R QR TSNVF MF + QIQEFKEAF MIDQN+DGFID +DL +M  SLG+  ++  ++EML ++ G +NFTMF+ LFGEK++GSDPEE++ NAF  FD D+ GF++EE+++DL+ +MGD +T E++ + +  AP++ GK +Y  F + IK GK +E
Sbjct:   13 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPDDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEE 168

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000066092.1 (SMESG000066092.1)

HSP 1 Score: 130.183 bits (326), Expect = 5.432e-37
Identity = 62/62 (100.00%), Postives = 62/62 (100.00%), Query Frame = 1

Query:  238 MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRT 423
            MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRT
Sbjct:    1 MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRT 62

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000034095.1 (SMESG000034095.1)

HSP 1 Score: 101.679 bits (252), Expect = 1.895e-25
Identity = 50/139 (35.97%), Postives = 88/139 (63.31%), Query Frame = 1

Query:  307 FPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSE----SPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTG-GKLDYEAFV 708
              + QI EFKEAF++ D++ DG I  ++L  +  SLG+ P++ ELQ+M++E      G ++F  F+ +   K+  +D EE +R AF++FD D  GF++   ++ ++ N+G+  T EE+ +  +EA + G G+++YE FV
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFV 143

The following BLAST results are available for this feature:

BLAST of Myosin regulatory light chain vs. Ensembl Human
BLAST of Myosin regulatory light chain vs. Ensembl Celegans
BLAST of Myosin regulatory light chain vs. Ensembl Fly
BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
BLAST of Myosin regulatory light chain vs. Ensembl Mouse
BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
BLAST of Myosin regulatory light chain vs. TrEMBL
BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
BLAST of Myosin regulatory light chain vs. Ensembl Yeast
BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
BLAST of Myosin regulatory light chain vs. Ensembl Medaka
BLAST of Myosin regulatory light chain vs. Planmine SMEST

BLAST of Myosin regulatory light chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
MYL9	1.009e-59	59.09	myosin light chain 9 [Source:HGNC Symbol;Acc:HGNC:...	[more]
MYL12B	4.467e-59	57.96	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]
MYL12B	4.467e-59	57.96	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]
MYL12B	4.467e-59	57.96	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]
MYL12B	4.467e-59	57.96	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mlc-4	4.775e-61	55.36	Myosin regulatory light chain [Source:UniProtKB/S...	[more]
mlc-2	1.087e-38	45.21	Myosin regulatory light chain 2 [Source:UniProtKB...	[more]
mlc-1	1.425e-38	45.21	Myosin regulatory light chain 1 [Source:UniProtKB...	[more]
cmd-1	1.808e-25	35.97	Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630...	[more]
cmd-1	1.145e-17	35.24	Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sqh	1.380e-59	57.32	gene:FBgn0003514 transcript:FBtr0339504	[more]
sqh	1.380e-59	57.32	gene:FBgn0003514 transcript:FBtr0339503	[more]
sqh	1.380e-59	57.32	gene:FBgn0003514 transcript:FBtr0070877	[more]
sqh	1.380e-59	57.32	gene:FBgn0003514 transcript:FBtr0339502	[more]
sqh	1.380e-59	57.32	gene:FBgn0003514 transcript:FBtr0345294	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
myl9a	1.495e-62	60.13	myosin, light chain 9a, regulatory [Source:ZFIN;Ac...	[more]
myl9a	1.495e-62	60.13	myosin, light chain 9a, regulatory [Source:ZFIN;Ac...	[more]
myl9a	1.495e-62	60.13	myosin, light chain 9a, regulatory [Source:ZFIN;Ac...	[more]
myl12.1	4.187e-61	58.86	myosin, light chain 12, genome duplicate 1 [Source...	[more]
myl12.1	4.187e-61	58.86	myosin, light chain 12, genome duplicate 1 [Source...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
myl9	9.449e-60	58.49	myosin light chain 9 [Source:NCBI gene;Acc:1001248...	[more]
myl12b	1.350e-57	57.32	myosin light chain 12B [Source:NCBI gene;Acc:44806...	[more]
ap5z1	1.866e-52	54.09	adaptor related protein complex 5 subunit zeta 1 [...	[more]
ap5z1	6.456e-52	51.79	adaptor related protein complex 5 subunit zeta 1 [...	[more]
myl10	1.080e-48	53.29	myosin light chain 10 [Source:NCBI gene;Acc:100170...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Myl9	2.249e-59	58.44	myosin, light polypeptide 9, regulatory [Source:MG...	[more]
Gm49909	3.067e-59	57.96	predicted gene, 49909 [Source:MGI Symbol;Acc:MGI:6...	[more]
Myl12b	3.067e-59	57.96	myosin, light chain 12B, regulatory [Source:MGI Sy...	[more]
Myl12b	3.067e-59	57.96	myosin, light chain 12B, regulatory [Source:MGI Sy...	[more]
Myl12a	1.016e-58	57.32	myosin, light chain 12A, regulatory, non-sarcomeri...	[more]

BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P08051\|MLR_SPISA	1.914e-69	64.38	Myosin regulatory light chain, smooth muscle OS=Sp...	[more]
sp\|P04113\|MLRA_MIZYE	3.949e-68	67.95	Myosin regulatory light chain A, smooth adductor m...	[more]
sp\|P04112\|MLRB_MIZYE	8.247e-65	65.81	Myosin regulatory light chain B, smooth adductor m...	[more]
sp\|Q09510\|MLRH_CAEEL	6.074e-60	55.36	Myosin regulatory light chain OS=Caenorhabditis el...	[more]
sp\|P24032\|MLRN_CHICK	1.610e-59	58.60	Myosin regulatory light chain 2, smooth muscle min...	[more]

BLAST of Myosin regulatory light chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
Q5DB55	1.541e-89	83.23	Myosin regulatory light chain 2, smooth muscle min...	[more]
A0A183AZT2	2.806e-89	82.82	Uncharacterized protein OS=Echinostoma caproni OX=...	[more]
A0A1S8WJA4	4.067e-89	79.43	EF hand OS=Opisthorchis viverrini OX=6198 GN=X801_...	[more]
A0A4V6RH67	5.043e-89	81.55	Uncharacterized protein OS=Opisthorchis felineus O...	[more]
A0A183MFP9	1.170e-88	85.26	Uncharacterized protein OS=Schistosoma margrebowie...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000048105.1	2.170e-62	51.87	pep primary_assembly:Astyanax_mexicanus-2.0:16:981...	[more]
myl9a	3.741e-62	59.49	myosin regulatory light polypeptide 9-like [Source...	[more]
ENSAMXT00000046001.1	1.750e-60	56.17	myosin regulatory light chain 2, smooth muscle min...	[more]
myl9b	2.686e-60	58.23	myosin regulatory light polypeptide 9 [Source:NCBI...	[more]
ENSAMXT00000051744.1	1.216e-54	56.13	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
myl9a	5.850e-50	52.69	myosin, light chain 9a, regulatory [Source:ZFIN;Ac...	[more]
ENSPMAT00000006008.1	2.411e-26	34.51	pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-...	[more]
CALM3	8.837e-26	34.51	calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449]	[more]
CALML3	9.200e-26	34.51	calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:145...	[more]
CETN1	1.194e-18	31.45	centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866]	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3


Match Name	E-value	Identity	Description
CMD1	8.199e-17	27.97	Calmodulin; Ca2+ binding protein that regulates Ca...	[more]
CDC31	2.780e-14	29.93	Calcium-binding component of the spindle pole body...	[more]
MLC2	8.836e-9	30.00	Regulatory light chain for the type II myosin Myo1...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO44829	4.973e-59	58.60	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO44767	9.143e-55	56.25	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO25626	5.749e-47	63.33	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8...	[more]
EDO42774	4.189e-43	54.23	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35925	2.801e-27	35.40	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
myl9a	5.778e-61	59.01	myosin regulatory light polypeptide 9 [Source:NCBI...	[more]
myl9a	5.961e-61	59.01	myosin regulatory light polypeptide 9 [Source:NCBI...	[more]
myl12.2	7.543e-61	58.23	myosin regulatory light chain 2, smooth muscle min...	[more]
ENSORLT00000014284.2	2.902e-59	57.69	myosin regulatory light chain 2, smooth muscle min...	[more]
MYL2	4.454e-49	49.08	myosin regulatory light chain 2, ventricular/cardi...	[more]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000066078.1	2.569e-117	100.00	SMESG000066078.1	[more]
SMESG000026825.1	6.393e-105	88.02	SMESG000026825.1	[more]
SMESG000000175.1	2.698e-55	53.21	SMESG000000175.1	[more]
SMESG000066092.1	5.432e-37	100.00	SMESG000066092.1	[more]
SMESG000034095.1	1.895e-25	35.97	SMESG000034095.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30009749 ID=SMED30009749|Name=Myosin regulatory light chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1350bp
CGTGATTTATGCGTAAAACAACTGCAGTATAAGGTAAACATAAACAAAAT
ATCCTCATCTAAAACCTGGGATTTATCATCGATGTGTGTGCAAGAATTGT
GCCGAACGGCAAGAAGCTGCTGATAATCGATTGAAATATGTGCCAATGCA
CCCGAGAATTTTTCTTCATATATTAATGGAATTTTTTGTATAATTAGTAA
GACATTTTCATTTAGGCATTCTCTCTTTCTTTTAAAAATGGCAGAAGAAG
GATCTCAATCTGGTGGAAAACGAGCACAACGAGCGACCTCAAATGTTTTC
GGAATGTTTCCTCAAAATCAGATACAAGAATTCAAAGAGGCGTTCACTAT
GATAGATCAAAACAGAGATGGATTCATTGATATAGAAGATTTGAAAGATA
TGTATGCGTCGTTAGGTCGAACCCCTAGTGATAAAGAATTGCAAGAAATG
TTGAGCGAATCGCCAGGACCTTTAAATTTTACAATGTTCATCAATCTTTT
TGGTGAAAAGCTATCCGGATCGGATCCTGAAGAATCATTAAGGAATGCCT
TTAAAATGTTTGATTCCGATAACAAAGGGTTTTTAGCCGAAGAATACATC
AAAGATTTATTGGAGAATATGGGTGACAATTTCACTGCTGAAGAGATTCG
ACAAACATGGAAAGAAGCACCACTTACTGGGGGGAAGTTAGATTATGAAG
CCTTCGTTAGACAAATCAAGCGCGGCAAAGATGACGAATAAAGCAAAAAA
ACAAATAATCAAAGATATAACGCTTTTAGTTAATTTCAAATTTTATTCGA
CAAAAAATACAATAAAAATCAAAATCAATTTTAGTGTAACATTATTTTAG
TTATATTTCTGAAATATTTTTGTTCTAAATACAATTTGTATTTTTTGTGA
AAACTATTTCGTTCCGGTCCTCTATTTAAACACGGTTGTTGGAAGCTGAT
TGTGAATTCTGTTGTATTTTTTTTCGTATATTTTAACCACTTCAGGATAA
TAGTTAATAATTGTTGTGAAATATAATTTCCGGAATGCTTCTAAAAAAAA
AAAAAAAAACAAATAATCAAAGATATAACGCTTTTAGTTAATTTCAAATT
TTATTCGACAAAAAATACAATAAAAATCAAAATCAATTTTAGTGTAACAT
TATTTTAGTTATATTTCTGAAATATTTTTGTTCTAAATACAATTTGTATT
TTTTGTGAAAACTATTTCGTTCCGGTCCTCTATTTAAACACGGTTGTTGG
AAGCTGATTGTGAATTCTGTTGTATTTTTTTTCGTATATTTTAACCACTT
CAGGATAATAGTTAATAATTGTTGTGAAATATAATTTCCGGAATGCTTCT

protein sequence of SMED30009749-orf-1

>SMED30009749-orf-1 ID=SMED30009749-orf-1|Name=SMED30009749-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=168bp
MAEEGSQSGGKRAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIE
DLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEES
LRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGK
LDYEAFVRQIKRGKDDE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000101	muscle cell
PLANA:0000428	musculature system
PLANA:0000478	pharynx musculature
PLANA:0007547	anterior pole progenitor cell

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **molecular function**
Term	Definition
GO:0005509	calcium ion binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 31..59 e-value: 3.8E-6 score: 36.4 coord: 100..128 e-value: 0.69 score: 19.0
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 31..60 e-value: 1.4E-7 score: 30.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 96..131 score: 11.696
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 27..62 score: 14.737
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 31..74 e-value: 1.16109E-7 score: 44.4609
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 94..161 e-value: 1.9E-17 score: 65.2
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 12..93 e-value: 8.4E-26 score: 91.8
None	No IPR available	PANTHER	PTHR23049:SF61		coord: 7..167
None	No IPR available	PANTHER	PTHR23049	MYOSIN REGULATORY LIGHT CHAIN 2	coord: 7..167
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 40..52
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 24..160