G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30009670
Length (bp)1287
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30009670) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30009670) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009670

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30009670h1SMcG0014970 SmedASXL_014020SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parenchymal cellSMED30009670h1SMcG0014970 dd_Smed_v4_8751_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Match: rbm38 (RNA binding motif protein 38 [Source:Xenbase;Acc:XB-GENE-489545])

HSP 1 Score: 49.6766 bits (117), Expect = 6.458e-6
Identity = 70/269 (26.02%), Postives = 112/269 (41.64%), Query Frame = 3 
Query:  162 GIFRGYFSPIVF----AVGLFGNVFVI-LTFTSKPLRNRF-AIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLK-ITTSYNITYKKCDVLAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWS 947
            G   G   P+V+    AVGL+GN  VI L + S   +N   A+Y + LA           L DD    GL F        Y          +C+L+  L     F S +     S DR L + +P+Q    +  KV+  V + V ++ FL+ +P++  SG+   T + +I + +   +  + ++    AL     +  I    +++V      G     KR R   ++ NRRQ G E        +  + F+L  FP   + II L W 
Sbjct:   31 GTSPGLLIPLVYLVVCAVGLWGNTLVIYLAWRSPAGQNSVTALYILNLA-----------LADDLFMLGLPFLAAQNALSYW----PFGSPACRLVMTLDAVNQFTSIFCLTVLSFDRYLAVVRPIQSAKWRKPKVAKCVNVTVWILSFLVVLPVVFFSGVPGDTGTCHIAWPEPAQMWRTGFILYTAALGFFCPLLVICICHLLIVAQVRSSG-----KRVR---VAPNRRQ-GPERKVTKMVALTVTAFVLCWFPFYALNIINLLWP 275
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183KKN2 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS15594 PE=4 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 4.850e-58
Identity = 121/301 (40.20%), Postives = 189/301 (62.79%), Query Frame = 3 
Query:  159 VGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYIY 1061
            VG FR Y  PI+  +G+ GN F+++ F  +   +RF+IYS+FLA +NI+ L + T IDDF GRGL +A   +   + FKLDT+S F CK ++Y+  TMYF SS+L V FS+DRLLTI+KP++F+S  H   +L  CILV  +G L + P++    L + TS    +  C +++E  +  ++  +  + +V+F +P  +VL +N  I  +LW++K  R  L+  +  ++ +EM R+ G+L +S+ FLLL  P++  ++IRL  +   V   +      I  DLSRLF+SVKDI YAV+F++Y
Sbjct:   14 VGSFRAYICPIIAIIGVIGNSFIVIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQ---FYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILVYSMGILSNSPLLFVQTLMVDTSSRTNF-TCRMISE--HPVAKFTITFETIVTFTIPFCLVLFLNVFICIELWKLKVNRTALLPADSSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCII--DLSRLFSSVKDITYAVNFFLY 306
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A430QMQ3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0001525 PE=4 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 5.061e-58
Identity = 121/301 (40.20%), Postives = 189/301 (62.79%), Query Frame = 3 
Query:  159 VGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYIY 1061
            VG FR Y  PI+  +G+ GN F+++ F  +   +RF+IYS+FLA +NI+ L + T IDDF GRGL +A   +   + FKLDT+S F CK ++Y+  TMYF SS+L V FS+DRLLTI+KP++F+S  H   +L  CILV  +G L + P++    L + TS    +  C +++E  +  ++  +  + +V+F +P  +VL +N  I  +LW++K  R  L+  +  ++ +EM R+ G+L +S+ FLLL  P++  ++IRL  +   V   +      I  DLSRLF+SVKDI YAV+F++Y
Sbjct:   14 VGSFRAYICPIIAIIGVIGNSFIVIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQ---FYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILVYSMGILSNSPLLFVQTLMVDTSSRTNF-TCRMISE--HPVAKFTITFETIVTFTIPFCLVLFLNVFICIELWKLKVNRTALLPADSSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCII--DLSRLFSSVKDITYAVNFFLY 306
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A094ZE57 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma haematobium OX=6185 GN=MS3_00775 PE=4 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 1.855e-57
Identity = 120/301 (39.87%), Postives = 188/301 (62.46%), Query Frame = 3 
Query:  159 VGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYIY 1061
            VG FR Y  PI+  +G+ GN F+++ F  +   +RF+IYS+FLA +NI+ L + T IDDF GRGL +A   +   + FKLDT+S F CK ++Y+  TMYF SS+L V FS+DRLLTI+KP++F+S  H   +L  CIL   +G L + P++    L + TS    +  C +++E  +  ++  +  + +V+F +P  +VL +N  I  +LW++K  R  L+  +  ++ +EM R+ G+L +S+ FLLL  P++  ++IRL  +   V   +      I  DLSRLF+SVKDI YAV+F++Y
Sbjct:   14 VGSFRAYICPIIAIIGVIGNSFIVIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQ---FYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILAYSMGILSNSPLLFVQTLMVDTSSRTNF-TCRMISE--HPVAKFTITFETIVTFTIPFCLVLFLNVFICIELWKLKVNRTALLPTDSSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCII--DLSRLFSSVKDITYAVNFFLY 306
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183L9P8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS523 PE=4 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 2.839e-57
Identity = 120/301 (39.87%), Postives = 189/301 (62.79%), Query Frame = 3 
Query:  159 VGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYIY 1061
            VG FR Y  PI+  +G+ GN F+I+ F  +   +RF+IYS+FLA +NI+ L + T IDDF GRGL +A   +   + FKLDT+S F CK ++Y+  TMYF SS+L V FS+DRLLTI+KP++F+S  H   ++  CILV ++G L + P++    L +  S    +  C +++E  +  ++  +  + +V+F +P  +VL +N  I  +LW++K  R  L+  +  ++ +EM R+ G+L +S+ FLLL  P++  ++IRL  +   V   +      I  DLSRLF+SVKDI YAV+F++Y
Sbjct:   14 VGSFRAYICPIIAIIGVIGNSFIIIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQ---FYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWAMIACILVYLMGILSNSPLLFVQTLMVDKSSRTNF-TCRMISE--HPVAKFTITFETIVTFTIPFCLVLFLNIFICIELWKLKVNRTALLPADSSRNCMEMGRVSGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCII--DLSRLFSSVKDITYAVNFFLY 306
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183P8J0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS10676 PE=4 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 3.261e-57
Identity = 120/301 (39.87%), Postives = 188/301 (62.46%), Query Frame = 3 
Query:  159 VGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYIY 1061
            VG FR Y  PI+  +G+ GN F+++ F  +   +RF+IYS+FLA +NI+ L + T IDDF GRGL +A   +   + FKLDT+S F CK ++Y+  TMYF SS+L V FS+DRLLTI+KP++F+S  H   +L  CILV  +G L + P++    L +  S    +  C +++E  +  ++  +  + +V+F +P  +VL +N  I  +LW++K  R  L+  +  ++ +EM R+ G+L +S+ FLLL  P++  ++IRL  +   V   +      I  DLSRLF+SVKDI YAV+F++Y
Sbjct:   14 VGSFRAYICPIIAIIGVIGNSFIVIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQ---FYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILVYSMGILSNSPLLFVQTLMVDKSSRTNF-TCRMISE--HPVAKFTITFETIVTFTIPFCLVLFLNVFICIELWKLKVNRTALLPADSSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCII--DLSRLFSSVKDITYAVNFFLY 306
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000011884.1 (SMESG000011884.1)

HSP 1 Score: 665.611 bits (1716), Expect = 0.000e+0
Identity = 371/373 (99.46%), Postives = 372/373 (99.73%), Query Frame = 3 
Query:  129 MLCKNSFKIAVGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYIYXXXXXXXXXXXXXRILGIVSCLNLNIFNQWKEFVEYQIFGENSKPKTSNSSLKATKIMETKN 1247
            MLCKNSFKIAVGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIG LMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKVVVSFIVPILIVLVINCVIIGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNS+EREQVEILGDLSRLFASVKDINYAVDFYIYLLFLNNFKWNFLNRILGIVSCLNLNIFNQWKEFVEYQIFGENSKPKTSNSSLKATKIMETKN
Sbjct:    1 MLCKNSFKIAVGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGSLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKVVVSFIVPILIVLVINCVIIGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSQEREQVEILGDLSRLFASVKDINYAVDFYIYLLFLNNFKWNFLNRILGIVSCLNLNIFNQWKEFVEYQIFGENSKPKTSNSSLKATKIMETKN 373
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000011885.1 (SMESG000011885.1)

HSP 1 Score: 328.946 bits (842), Expect = 6.881e-110
Identity = 188/357 (52.66%), Postives = 269/357 (75.35%), Query Frame = 3 
Query:  129 MLCKNSFKIAVGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYIYXXXXXXXXXXXXXRILGIVSCL--NLNIFNQWKEFVEYQIFGENSK 1193
            M+C+  FK  VG+FRGY SP++F  GL GN+FVI  FT KPLRNRFAIYS+FLAGS+++IL V T +DDF GRGLA+A +G+C+ + FKLDT+S+ SCK +QY+P+TMY ISSW+FV FSLDR LTIY+P+ F+S  H+K SL  C +V V+ F++++P+++ SGL+I  S N+T+ KCD+L ES +  +RI LY+K++ SFI+P  I+L++N  II  L++IK+ R  L+  +R ++GLEMNR+IG+L +SS FLLL FPLII M +R  W+ +  +N  ++  +E LGDLSRLF+S+KDI+YA DFYIY+LFLNNF+W FL+ ++ I+ C        N+WK  V++++ G + +
Sbjct:    1 MICETIFKELVGLFRGYLSPVIFVFGLVGNIFVIRIFTDKPLRNRFAIYSIFLAGSHVIILVVDTFLDDFIGRGLAYAAYGKCNNHGFKLDTISNASCKTMQYIPDTMYSISSWIFVVFSLDRALTIYRPLLFYSTLHTKKSLIFCCVVVVLSFIINVPLVVGSGLQINKSQNVTFVKCDLLTESQFYITRIELYIKMIFSFILPTFIILILNSFIIIILFQIKKSRRDLMPTDRAKNGLEMNRVIGHLGLSSFFLLLAFPLIITMFVRTNWTGRANENLNDKMFLEKLGDLSRLFSSLKDISYAADFYIYVLFLNNFRWYFLDHLIDILRCCIPKSKRLNEWKFTVQFRLSGYSRR 357
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000003388.1 (SMESG000003388.1)

HSP 1 Score: 191.045 bits (484), Expect = 2.392e-56
Identity = 125/325 (38.46%), Postives = 201/325 (61.85%), Query Frame = 3 
Query:  141 NSFKIAVGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYL--FKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGL-KITTSYNITYKKCDVLAESSY-LASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLI----SKNRRQSGL----------EMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYIY 1061
            + F++ VG FR Y +P+V  VG+FGN+FVIL F  +   +RF++Y + LA   +V L V + +DDF GRGL+  +      YL  FK DT SD SCK+++Y+P TM+FI+S++ V FS+DRLLTI++P++F S  + K S+  C+   + GF ++IP++LS GL K    + IT  KC V+A  +    +   LY+ ++ SF +P  IVL++N +I  KLW++   R  +     + + + S +          E+NR++G+L V+  FLLL  PL+I++I+R++  D K+  +           LSRLF+S+KD+N++ +F+IY
Sbjct:   16 HEFEVVVGSFRAYVTPVVAVVGIFGNIFVILIFYFETKLSRFSVYCISLAFVQLVALIVNSFMDDFLGRGLSHVL----GYYLLAFKWDTHSDMSCKMMEYVPNTMFFIASFVTVLFSIDRLLTIHQPLKFHSVYYIKYSIMACVGTFLFGFAINIPLLLSYGLIKQENEFGITL-KCTVVATGARKFVADFGLYLVLLGSFTIPTFIVLILNTLISIKLWQLHLNRAEMTKSFDTSDSKSSAINPSLMKNQEKELNRVLGHLAVNVVFLLLTMPLVIVIILRMYSDDNKMYLT-NLYLYNSFQHLSRLFSSLKDVNFSCEFFIY 334
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000070788.1 (SMESG000070788.1)

HSP 1 Score: 185.267 bits (469), Expect = 7.224e-54
Identity = 123/331 (37.16%), Postives = 198/331 (59.82%), Query Frame = 3 
Query:  159 VGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYK---KCDVLAESS--YLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRK---------------------RMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQ----VEILGDLSRLFASVKDINYAVDFYIY 1061
            VG+FRG+ +P++  +G  GN F+IL F+ +   +RF+IY + LA ++ + L     +DDF GRGLA+       +   KLD  S+ SCKL++Y+P ++YF SS + V FS+DRLLTIY+P++ F+  +  +S+F+C      GF+++IP+ +S  L +   Y+  ++   KCD+L   S   + SR A+YMK+  +FI P  IVL++N VII K   + RK                       +  SK + Q G +++RI+G++ V+S FL L  PL+I++I+R++      +N+++RE        L  LSRLF+S+KDINYA +F++Y
Sbjct:   16 VGVFRGFITPLLCVIGFIGNGFIILIFSKETKISRFSIYCITLAITHSLALFTNGFLDDFLGRGLAYVTHNSHSV--CKLDVFSNESCKLMEYIPNSLYFASSAIIVVFSIDRLLTIYQPIKMFAAYYLNLSIFICGFCVSAGFILNIPLFMSYSL-VKDKYHALHEWEYKCDMLVGKSGTEIISRFAIYMKLFGAFICPTFIVLILNTVIIYKFCTLNRKFNIYNDRNRNLVNKSTLKRHSTGIFFSKTQTQDG-DLSRIVGHIAVNSAFLSLTLPLVIVIILRIYS-----ENTDKREHGLYYYNSLKHLSRLFSSIKDINYASNFFVY 337
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000048976.1 (SMESG000048976.1)

HSP 1 Score: 177.178 bits (448), Expect = 9.895e-51
Identity = 112/310 (36.13%), Postives = 190/310 (61.29%), Query Frame = 3 
Query:  150 KIAVGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYSVFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLF---KLDTVSDFSCKLIQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVTVIGFLMSIPIILSSGLKITTSYNITYK--KCDV--LAESSYLASRIALYMKXXXXXXXXXXXXXXXXXXXXGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLIVSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVKDINYAVDFYI 1058
            +I+V IFR YFS  +  +G+ GN  V+  F  +  ++RF+IY + LA  +IVIL   + +DDF GRG+ +        Y F   K+D  SDF CKL++Y+P   Y  SSW+ + FS DR++TI++P++F S  +   +    ++  +  FL++I I++     +   + +     KC++  L +++    +  LY+K++ +F++P  +VL++N +I  KLW+I+  R  L+  + + + LEM+R+IG+L +SSCF+L+  PL+I +I+R+  SD++       E  + L  LSRLF+SVKDINYA DF++
Sbjct:   23 EISVDIFRAYFSIWISIIGITGNFIVLFVFAKERQKSRFSIYCMLLAMVHIVILFTNSFLDDFLGRGVTYL-----SNYTFTSSKIDIKSDFLCKLMEYVPNAAYLASSWIVIIFSCDRIITIFRPLKFMSIYYQLYAWAGSVVTIIFAFLLNICIVIIYEKILDKKHGLAMSNIKCEISSLYKATDFICKYCLYLKLMGAFVIPNFLVLIMNFIICVKLWKIQTTRCKLLPSDSKNNVLEMSRVIGHLALSSCFILMTMPLVIAIILRM-NSDRRNYKKLYPEYHDFLKHLSRLFSSVKDINYASDFFL 326
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rbm386.458e-626.02RNA binding motif protein 38 [Source:Xenbase;Acc:X... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183KKN24.850e-5840.20G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A430QMQ35.061e-5840.20G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A094ZE571.855e-5739.87G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183L9P82.839e-5739.87G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183P8J03.261e-5739.87G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000011884.10.000e+099.46SMESG000011884.1[more]
SMESG000011885.16.881e-11052.66SMESG000011885.1[more]
SMESG000003388.12.392e-5638.46SMESG000003388.1[more]
SMESG000070788.17.224e-5437.16SMESG000070788.1[more]
SMESG000048976.19.895e-5136.13SMESG000048976.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009670 ID=SMED30009670|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1287bp
GTGAATTTAAACAAACTTATTTTAATTAAAAAATTAGCAGTTTTACTAAT
AATAGTATCTATTTGCATATAAATAATATAATTTACGGCATAGGATTAAT
TGAAAATCTGAATGTGAAATAACAATAGATGCTGTGCAAAAATTCTTTCA
AAATCGCCGTTGGAATATTCCGTGGATATTTTTCGCCGATCGTTTTTGCT
GTTGGCTTATTTGGAAATGTTTTTGTCATTTTGACGTTCACGTCGAAACC
TTTGAGAAATCGGTTTGCGATATATAGCGTTTTTTTGGCCGGTTCGAATA
TTGTGATTTTAACAGTCGGAACGCTAATTGATGATTTTACGGGTCGAGGC
TTAGCATTTGCGATTTTCGGACGTTGTGACATTTATTTGTTTAAATTGGA
CACGGTTTCCGATTTCAGTTGCAAACTCATTCAGTATTTACCAGAAACGA
TGTATTTCATTTCATCTTGGCTGTTTGTTGCTTTTTCTTTGGATCGGCTT
TTGACTATTTATAAACCGGTCCAGTTTTTTTCCAATCAACATTCAAAAGT
TTCATTATTTGTTTGTATTCTAGTGACAGTTATTGGTTTTTTGATGAGCA
TCCCAATTATTCTGAGTTCAGGCTTGAAGATAACAACGAGTTATAACATC
ACATATAAGAAATGCGATGTCTTAGCTGAATCTTCATATTTGGCATCTCG
AATAGCTCTTTATATGAAAGTAGTAGTTTCTTTTATCGTTCCCATTCTAA
TTGTCCTAGTTATTAACTGTGTGATAATAGGAAAGTTGTGGGAAATAAAG
AGAAAGAGAATGATGTTAATTTCGAAGAACAGGCGACAGAGTGGACTAGA
AATGAACAGAATAATTGGCTATTTGATAGTAAGCTCTTGTTTTCTATTAT
TGGTTTTTCCTTTGATCATCATTATGATCATTCGGTTGTGGTGGAGTGAT
AAAAAAGTAAAGAATTCTGAAGAAAGAGAACAAGTTGAAATTTTGGGAGA
TTTATCAAGACTCTTTGCATCTGTCAAAGATATCAATTACGCTGTAGATT
TTTATATTTATTTGCTATTCTTGAATAACTTTAAATGGAATTTCCTTAAC
AGAATTCTTGGTATAGTGAGCTGTTTAAATTTGAATATATTCAATCAGTG
GAAAGAATTTGTAGAGTATCAAATTTTTGGAGAAAATTCCAAACCTAAAA
CTTCGAATAGTTCCTTAAAGGCCACAAAAATCATGGAAACGAAAAATTAA
TAATTAATACACGATTTATACAATTTTTGAAAAAACT
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protein sequence of SMED30009670-orf-1

>SMED30009670-orf-1 ID=SMED30009670-orf-1|Name=SMED30009670-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=374bp
MLCKNSFKIAVGIFRGYFSPIVFAVGLFGNVFVILTFTSKPLRNRFAIYS
VFLAGSNIVILTVGTLIDDFTGRGLAFAIFGRCDIYLFKLDTVSDFSCKL
IQYLPETMYFISSWLFVAFSLDRLLTIYKPVQFFSNQHSKVSLFVCILVT
VIGFLMSIPIILSSGLKITTSYNITYKKCDVLAESSYLASRIALYMKVVV
SFIVPILIVLVINCVIIGKLWEIKRKRMMLISKNRRQSGLEMNRIIGYLI
VSSCFLLLVFPLIIIMIIRLWWSDKKVKNSEEREQVEILGDLSRLFASVK
DINYAVDFYIYLLFLNNFKWNFLNRILGIVSCLNLNIFNQWKEFVEYQIF
GENSKPKTSNSSLKATKIMETKN*
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protein sequence of SMED30009670-orf-2

>SMED30009670-orf-2 ID=SMED30009670-orf-2|Name=SMED30009670-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MSQRPKIANAKPRPVKSSISVPTVKITIFEPAKKTLYIANRFLKGFDVNV
KMTKTFPNKPTAKTIGEKYPRNIPTAILKEFLHSIYCYFTFRFSINPMP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 105..127
score: 23.08
coord: 194..217
score: 19.82
coord: 14..38
score: 23.79
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 29..276
e-value: 2.8E-14
score: 53.1
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 3..333
e-value: 3.0E-36
score: 126.9
NoneNo IPR availablePANTHERPTHR24243G-PROTEIN COUPLED RECEPTORcoord: 12..331
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 13..314
e-value: 6.90848E-25
score: 100.785
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 13..328
NoneNo IPR availableTMHMMTMhelixcoord: 45..67
NoneNo IPR availableTMHMMTMhelixcoord: 13..35
NoneNo IPR availableTMHMMTMhelixcoord: 245..267
NoneNo IPR availableTMHMMTMhelixcoord: 199..221
NoneNo IPR availableTMHMMTMhelixcoord: 141..163
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 29..311
score: 23.762