SMED30009623

Overview
NameSMED30009623
Smed IDSMED30009623
Length (bp)915
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009623 (SMED30009623) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009623 (SMED30009623) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009623 (SMED30009623) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009623 (SMED30009623) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009623

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 22

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30009623h1SMcG0005835 Contig7058newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005835 Contig7058uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005836 Contig7058newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005836 Contig7058uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005839 Contig92newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005839 Contig92uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005834 Contig92newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005834 Contig92uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005911 Contig92newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009623h1SMcG0005911 Contig92uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30009623h1SMcG0005840 dd_Smed_v4_9340_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30009623h1SMcG0005840 dd_Smed_v4_9340_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005823 Contig19411uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005823 Contig19411newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005817 Contig10395uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005817 Contig10395newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005834 Contig10395uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005834 Contig10395newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005834 Contig9655uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005834 Contig9655newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005839 Contig9655uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009623h1SMcG0005839 Contig9655newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009623 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.017690:7064..8561 -3331
v31.009145:31828..32853 -3265
v31.015331:7579..8629 -3177
v31.010883:16225..17231 +3172
v31.002942:66899..68023 +3025
v31.015478:8801..9675 -2597
Homology
[Close]
BLAST of SMED30009623 vs. Planmine SMEST
Match: SMESG000036060.1 (SMESG000036060.1)

HSP 1 Score: 307.76 bits (787), Expect = 1.278e-99
Identity = 193/220 (87.73%), Postives = 204/220 (92.73%), Query Frame = 3 
Query:  249 RYSDRGAGLRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHTELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKEKLKLSXXXXXXXXXXXXIFILQMXXXXXXXXXXXXXXXILQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAK 908
            +YS+ GAG RQNSR H DNYF+TNSADIYGTMPKWNDDYQSRNRD H+ELDRHERGIE FGEEIEDLRIRSNKSERKNGR QEELDDCK KLKLSQERNLQLENENEIFIL+M+NSKNSNEKL+SE KILQNETNQYL RINQLQSALKESKGIN RLQQDFDDCKVKLNHS+RRNSQLE+AN ++ QI  NQC NCAESTRQLQNQKSKVKKKIEELAK
Sbjct:  134 KYSEGGAGSRQNSRFHGDNYFATNSADIYGTMPKWNDDYQSRNRDWHSELDRHERGIEIFGEEIEDLRIRSNKSERKNGRSQEELDDCKAKLKLSQERNLQLENENEIFILEMENSKNSNEKLRSECKILQNETNQYLTRINQLQSALKESKGINLRLQQDFDDCKVKLNHSQRRNSQLETANQYEHQINRNQCANCAESTRQLQNQKSKVKKKIEELAK 353

HSP 2 Score: 68.9366 bits (167), Expect = 8.799e-13
Identity = 45/93 (48.39%), Postives = 64/93 (68.82%), Query Frame = 3 
Query:  636 LQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            L+ + ++YLA IN+L+S  +     N RLQQ+FD    K  HS+R NSQLESAN FKSQ+  ++ EN +E  +QL+N+K   K+K+EE+ KDF
Sbjct:  487 LKFQCDKYLAEINKLESLDR----TNRRLQQEFD---AKSKHSER-NSQLESANLFKSQMDRSRFENNSELIKQLENEKETDKRKVEEITKDF 571

HSP 3 Score: 57.7658 bits (138), Expect = 4.833e-9
Identity = 40/97 (41.24%), Postives = 51/97 (52.58%), Query Frame = 3 
Query:  636 LQNETNQ----YLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            LQN+ N     + A+I QLQSALKES    G LQ+DF+DCK KL HS++RN QLE                         N+ +  K+ IEE+ KDF
Sbjct:  599 LQNDNNNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQKRNIQLE-------------------------NEMAVKKRTIEEITKDF 670
[Close]
BLAST of SMED30009623 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1)

HSP 1 Score: 308.145 bits (788), Expect = 6.024e-97
Identity = 194/220 (88.18%), Postives = 206/220 (93.64%), Query Frame = 3 
Query:  249 RYSDRGAGLRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHTELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKEKLKLSXXXXXXXXXXXXIFILQMXXXXXXXXXXXXXXXILQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAK 908
            +YSD GA  RQNSR H DNYFSTNSADIYGTMP+WN+DYQSRN D H+ELDRHERGIE FGEEIEDLRIRSNKSERKNGRLQEELDD K KLKLSQERNLQLENENEIFIL+M+NSKNSNEKL+SE KILQNETNQYLA+INQLQSALKESKGINGRLQQDFDDCKVKLNHS+RRNSQLESAN ++ QIGTN+C NCAESTRQLQNQKSKVKKKIEELAK
Sbjct:  131 KYSDGGARSRQNSRFHGDNYFSTNSADIYGTMPEWNNDYQSRNSDWHSELDRHERGIEIFGEEIEDLRIRSNKSERKNGRLQEELDDWKAKLKLSQERNLQLENENEIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNKCANCAESTRQLQNQKSKVKKKIEELAK 350

HSP 2 Score: 84.7297 bits (208), Expect = 6.019e-18
Identity = 51/93 (54.84%), Postives = 67/93 (72.04%), Query Frame = 3 
Query:  636 LQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            L+ + N+YLA IN+L+S LKES   N RLQQ+F+    K  HS+R NSQLESAN FKSQ+  N+ EN AE  +QL+N+K   K+K+EE+ KDF
Sbjct:  426 LKFQCNKYLAEINKLESVLKESDRTNRRLQQEFE---AKSKHSER-NSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEIIKDF 514

HSP 3 Score: 62.3882 bits (150), Expect = 1.526e-10
Identity = 69/171 (40.35%), Postives = 90/171 (52.63%), Query Frame = 3 
Query:  465 KSERKNGRLQEELDDCKEKLKLSXXXXXXXXXXXXI-----------FILQ-------MXXXXXXXXXXXXXXXILQNETN---QYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            +S+RK G LQ + +DCKEKLK SQERNLQLENE  +           F L+       ++  K    + +++ K LQNE N    + A+I QLQSALKES    G LQ+DF+DCK KL HS+ RN QLE                         N+ +  K+ IEE+ KDF
Sbjct:  566 ESDRKMGSLQRDFEDCKEKLKHSQERNLQLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQNENNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLE-------------------------NEMAVNKRTIEEITKDF 711

HSP 4 Score: 55.8398 bits (133), Expect = 1.757e-8
Identity = 40/97 (41.24%), Postives = 50/97 (51.55%), Query Frame = 3 
Query:  636 LQNETNQ----YLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            LQNE +     + A+I QLQSALKES    G LQ+DF+DCK KL HS+ RN QLE                         N+ +  K+ IEE+ KDF
Sbjct:  542 LQNENDNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLE-------------------------NEMAVNKRTIEEITKDF 613
[Close]
BLAST of SMED30009623 vs. Planmine SMEST
Match: SMESG000036102.1 (SMESG000036102.1)

HSP 1 Score: 307.76 bits (787), Expect = 2.669e-96
Identity = 194/220 (88.18%), Postives = 206/220 (93.64%), Query Frame = 3 
Query:  249 RYSDRGAGLRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHTELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKEKLKLSXXXXXXXXXXXXIFILQMXXXXXXXXXXXXXXXILQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAK 908
            +YSD GA  RQNSR H DNYFSTNSADIYGTMP+WN+DYQSRN D H+ELDRHERGIE FGEEIEDLRIRSNKSERKNGRLQEELDD K KLKLSQERNLQLENENEIFIL+M+NSKNSNEKL+SE KILQNETNQYLA+INQLQSALKESKGINGRLQQDFDDCKVKLNHS+RRNSQLESAN ++ QIGTN+C NCAESTRQLQNQKSKVKKKIEELAK
Sbjct:  131 KYSDGGARSRQNSRFHGDNYFSTNSADIYGTMPEWNNDYQSRNSDWHSELDRHERGIEIFGEEIEDLRIRSNKSERKNGRLQEELDDWKAKLKLSQERNLQLENENEIFILEMENSKNSNEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNKCANCAESTRQLQNQKSKVKKKIEELAK 350

HSP 2 Score: 84.7297 bits (208), Expect = 6.151e-18
Identity = 51/93 (54.84%), Postives = 67/93 (72.04%), Query Frame = 3 
Query:  636 LQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            L+ + N+YLA IN+L+S LKES   N RLQQ+F+    K  HS+R NSQLESAN FKSQ+  N+ EN AE  +QL+N+K   K+K+EE+ KDF
Sbjct:  426 LKFQCNKYLAEINKLESVLKESDRTNRRLQQEFE---AKSKHSER-NSQLESANLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEIIKDF 514

HSP 3 Score: 62.3882 bits (150), Expect = 1.513e-10
Identity = 69/171 (40.35%), Postives = 90/171 (52.63%), Query Frame = 3 
Query:  465 KSERKNGRLQEELDDCKEKLKLSXXXXXXXXXXXXI-----------FILQ-------MXXXXXXXXXXXXXXXILQNETN---QYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            +S+RK G LQ + +DCKEKLK SQERNLQLENE  +           F L+       ++  K    + +++ K LQNE N    + A+I QLQSALKES    G LQ+DF+DCK KL HS+ RN QLE                         N+ +  K+ IEE+ KDF
Sbjct:  566 ESDRKMGSLQRDFEDCKEKLKHSQERNLQLENEMAVNKRTIEEITKDFQLKKEDSNRSIEKLKLQLSQSENKCKSLQNENNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLE-------------------------NEMAVNKRTIEEITKDF 711

HSP 4 Score: 56.225 bits (134), Expect = 1.619e-8
Identity = 40/97 (41.24%), Postives = 50/97 (51.55%), Query Frame = 3 
Query:  636 LQNETNQ----YLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            LQNE +     + A+I QLQSALKES    G LQ+DF+DCK KL HS+ RN QLE                         N+ +  K+ IEE+ KDF
Sbjct:  542 LQNENDNIKSGFAAQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLE-------------------------NEMAVNKRTIEEITKDF 613
[Close]
BLAST of SMED30009623 vs. Planmine SMEST
Match: SMESG000038532.1 (SMESG000038532.1)

HSP 1 Score: 300.827 bits (769), Expect = 1.223e-93
Identity = 187/205 (91.22%), Postives = 195/205 (95.12%), Query Frame = 3 
Query:  264 GAGLRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHTELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKEKLKLSXXXXXXXXXXXXIFILQMXXXXXXXXXXXXXXXILQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSK 878
            GA  RQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRD H+ELDRHERGIERFGEEIEDLRIRSNKSERKNGRLQEELDDCK KLKLSQERN QLENENEIFIL+M+NS NSNEKL+SE KILQNETNQYLA+INQLQSALKESKGINGRLQQDFDDCKVKLNHS+RRNSQLESAN ++ QIGTNQC NCAESTRQLQNQKSK
Sbjct:  124 GARSRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDWHSELDRHERGIERFGEEIEDLRIRSNKSERKNGRLQEELDDCKAKLKLSQERNSQLENENEIFILEMENSNNSNEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNQCANCAESTRQLQNQKSK 328

HSP 2 Score: 86.6557 bits (213), Expect = 1.241e-18
Identity = 52/93 (55.91%), Postives = 67/93 (72.04%), Query Frame = 3 
Query:  636 LQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            L+ + ++YLA IN+L+S LKES   N RLQQ+FD    K  HS+R NSQLESAN FKSQ+  N+ EN AE  +QL+N+K   KKK+EE+ KDF
Sbjct:  593 LKFQCDKYLAEINKLESVLKESDRTNSRLQQEFD---AKSKHSER-NSQLESANLFKSQMDRNRFENNAELIKQLENEKEIDKKKVEEITKDF 681

HSP 3 Score: 82.8037 bits (203), Expect = 2.881e-17
Identity = 49/93 (52.69%), Postives = 62/93 (66.67%), Query Frame = 3 
Query:  636 LQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            L+ + ++YLA IN+L+S LKES   N RLQQ+ D    K  HS   NSQLES N ++ QI  NQC NC E  +QLQN+  +VK KIEE+ KDF
Sbjct:  373 LKFQHDKYLAEINKLESMLKESDRTNRRLQQEID---AKSKHSDW-NSQLESTNQYEHQINRNQCTNCEELIKQLQNENERVKSKIEEITKDF 461
[Close]
BLAST of SMED30009623 vs. Planmine SMEST
Match: SMESG000038532.1 (SMESG000038532.1)

HSP 1 Score: 300.442 bits (768), Expect = 1.897e-93
Identity = 187/205 (91.22%), Postives = 195/205 (95.12%), Query Frame = 3 
Query:  264 GAGLRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHTELDRHXXXXXXXXXXXXDLRIRSNKSERKNGRLQEELDDCKEKLKLSXXXXXXXXXXXXIFILQMXXXXXXXXXXXXXXXILQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSK 878
            GA  RQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRD H+ELDRHERGIERFGEEIEDLRIRSNKSERKNGRLQEELDDCK KLKLSQERN QLENENEIFIL+M+NS NSNEKL+SE KILQNETNQYLA+INQLQSALKESKGINGRLQQDFDDCKVKLNHS+RRNSQLESAN ++ QIGTNQC NCAESTRQLQNQKSK
Sbjct:  124 GARSRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDWHSELDRHERGIERFGEEIEDLRIRSNKSERKNGRLQEELDDCKAKLKLSQERNSQLENENEIFILEMENSNNSNEKLRSECKILQNETNQYLAQINQLQSALKESKGINGRLQQDFDDCKVKLNHSQRRNSQLESANQYEHQIGTNQCANCAESTRQLQNQKSK 328

HSP 2 Score: 86.6557 bits (213), Expect = 1.430e-18
Identity = 52/93 (55.91%), Postives = 67/93 (72.04%), Query Frame = 3 
Query:  636 LQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            L+ + ++YLA IN+L+S LKES   N RLQQ+FD    K  HS+R NSQLESAN FKSQ+  N+ EN AE  +QL+N+K   KKK+EE+ KDF
Sbjct:  593 LKFQCDKYLAEINKLESVLKESDRTNSRLQQEFD---AKSKHSER-NSQLESANLFKSQMDRNRFENNAELIKQLENEKEIDKKKVEEITKDF 681

HSP 3 Score: 82.4185 bits (202), Expect = 3.225e-17
Identity = 49/93 (52.69%), Postives = 62/93 (66.67%), Query Frame = 3 
Query:  636 LQNETNQYLARINQLQSALKESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCAESTRQLQNQKSKVKKKIEELAKDF 914
            L+ + ++YLA IN+L+S LKES   N RLQQ+ D    K  HS   NSQLES N ++ QI  NQC NC E  +QLQN+  +VK KIEE+ KDF
Sbjct:  373 LKFQHDKYLAEINKLESMLKESDRTNRRLQQEID---AKSKHSDW-NSQLESTNQYEHQINRNQCTNCEELIKQLQNENERVKSKIEEITKDF 461
The following BLAST results are available for this feature:
BLAST of SMED30009623 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009623 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000036060.11.278e-9987.73SMESG000036060.1[more]
SMESG000036102.16.024e-9788.18SMESG000036102.1[more]
SMESG000036102.12.669e-9688.18SMESG000036102.1[more]
SMESG000038532.11.223e-9391.22SMESG000038532.1[more]
SMESG000038532.11.897e-9391.22SMESG000038532.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009623 ID=SMED30009623|Name=SMED30009623|organism=Schmidtea mediterranea sexual|type=transcript|length=915bp
ATCTATATAAATCTTATATGTTGCACGTCCTTTGGTAGAGAAAATACAGA
GCCAGCCTCAATTTTCCTCAGTCAACACCTCAACTTAATAATATGAAAAA
AAATCTTTCAAAAAGTTACGAATATTTGAATTATCAAAATAATCCATTTA
AGCATGAAAGAAATTCAATTTCAATTGATAGGAACAAGCAAAACTCATTA
TCAAGATCTTCATCTGAATTTATCATAGAATCTATAGATGTCAATCCTAG
ATATTCTGACCGAGGGGCTGGATTGAGGCAAAACAGCAGATCCCATCGAG
ACAATTACTTTTCTACCAACTCGGCTGATATTTATGGCACAATGCCCAAG
TGGAATGATGATTATCAATCACGGAACAGAGATTTGCATACAGAATTAGA
TAGGCACGAGAGGGGGATAGAAAGATTTGGAGAAGAAATAGAGGATTTGA
GAATAAGATCGAATAAATCAGAAAGAAAAAATGGAAGATTGCAGGAAGAG
CTTGATGATTGCAAAGAAAAATTAAAACTTTCACAGGAGAGAAATTTACA
ATTAGAGAATGAAAATGAGATATTTATATTGCAAATGAAGAATTCAAAAA
ATTCTAATGAGAAACTGAAATCGGAATTCAAAATCTTACAGAATGAAACT
AATCAATATTTGGCACGAATTAACCAATTGCAATCAGCATTGAAGGAATC
AAAAGGAATAAATGGAAGATTGCAGCAAGATTTTGATGATTGCAAAGTGA
AATTGAATCATTCTAAGAGAAGGAATTCACAATTGGAATCTGCAAATTAC
TTCAAATCACAAATCGGCACAAATCAATGTGAAAATTGTGCAGAATCGAC
AAGACAATTGCAAAATCAAAAATCGAAAGTTAAAAAAAAAATTGAAGAAC
TTGCAAAAGATTTCA
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protein sequence of SMED30009623-orf-1

>SMED30009623-orf-1 ID=SMED30009623-orf-1|Name=SMED30009623-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=274bp
MKKNLSKSYEYLNYQNNPFKHERNSISIDRNKQNSLSRSSSEFIIESIDV
NPRYSDRGAGLRQNSRSHRDNYFSTNSADIYGTMPKWNDDYQSRNRDLHT
ELDRHERGIERFGEEIEDLRIRSNKSERKNGRLQEELDDCKEKLKLSQER
NLQLENENEIFILQMKNSKNSNEKLKSEFKILQNETNQYLARINQLQSAL
KESKGINGRLQQDFDDCKVKLNHSKRRNSQLESANYFKSQIGTNQCENCA
ESTRQLQNQKSKVKKKIEELAKDF
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 82..116
NoneNo IPR availableCOILSCoilCoilcoord: 33..74
NoneNo IPR availableCOILSCoilCoilcoord: 166..190
NoneNo IPR availableSUPERFAMILYSSF57997Tropomyosincoord: 9..187