SMED30009612

Overview
NameSMED30009612
Smed IDSMED30009612
Length (bp)1050
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009612 (SMED30009612) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009612 (SMED30009612) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009612 (SMED30009612) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009612 (SMED30009612) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009612

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30009612h1SMcG0018517 dd_Smed_v4_12983_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30009612h1SMcG0018517 dd_Smed_v6_12983_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009612 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000456:75842..80941 +4964
Homology
[Close]
BLAST of SMED30009612 vs. Ensembl Human
Match: PRR5L (proline rich 5 like [Source:HGNC Symbol;Acc:HGNC:25878])

HSP 1 Score: 52.7582 bits (125), Expect = 4.576e-7
Identity = 44/183 (24.04%), Postives = 88/183 (48.09%), Query Frame = 3 
Query:  279 SKNENWQKVQEALTDSLLGKSIKNLSEVLCC---IRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSG 800
            S +  W  VQ A+ +   G  +++ +E+      IR+L +      I DY++NQ+L   L  ++ +I           L E W  FF ++LP +  I   ++  +  + +++L  F R L  ++     L+   +S   SS+++ M L+LQ   +     ++  ++ EE+   +++P +G SG
Sbjct:   48 SSSSAWNSVQTAVINVFKGGGLQS-NELYALNENIRRLLKSELGSFITDYFQNQLLAKGLFFVEEKIKLCEGENRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGF-RDLVLLKVKLGDLLLLAQSKLPSSIVQ-MLLILQSVHEPTGP-SESYLQLEELVKQVVSPFLGISG 226
[Close]
BLAST of SMED30009612 vs. Ensembl Human
Match: PRR5L (proline rich 5 like [Source:HGNC Symbol;Acc:HGNC:25878])

HSP 1 Score: 52.7582 bits (125), Expect = 4.576e-7
Identity = 44/183 (24.04%), Postives = 88/183 (48.09%), Query Frame = 3 
Query:  279 SKNENWQKVQEALTDSLLGKSIKNLSEVLCC---IRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSG 800
            S +  W  VQ A+ +   G  +++ +E+      IR+L +      I DY++NQ+L   L  ++ +I           L E W  FF ++LP +  I   ++  +  + +++L  F R L  ++     L+   +S   SS+++ M L+LQ   +     ++  ++ EE+   +++P +G SG
Sbjct:   48 SSSSAWNSVQTAVINVFKGGGLQS-NELYALNENIRRLLKSELGSFITDYFQNQLLAKGLFFVEEKIKLCEGENRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGF-RDLVLLKVKLGDLLLLAQSKLPSSIVQ-MLLILQSVHEPTGP-SESYLQLEELVKQVVSPFLGISG 226
[Close]
BLAST of SMED30009612 vs. Ensembl Xenopus
Match: PRR5L (uncharacterized loc100145110 [Source:Xenbase;Acc:XB-GENE-5780652])

HSP 1 Score: 50.0618 bits (118), Expect = 3.477e-6
Identity = 48/213 (22.54%), Postives = 89/213 (41.78%), Query Frame = 3 
Query:  294 WQKVQEALTDSLLGKSIKNLSEVLCC---IRQLDEQYASKIIVDYYENQILKNTLQKLQRRIT--NARN----LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIFVDGRLIHKCKNVKIYQNHFDSDSKKQMKTKRIP 905
            W  VQ A+ +   G  ++ ++E+      IR L +      I DY++NQ+L   L  ++ ++      N    L E W  FF + LP +  I   ++  +  + ++ L  F+  +    +L K L   + SS     +  M LVLQ   +      +  +  EE+  ++++P VG  G          C N+     H    S+   + + +P
Sbjct:   85 WNSVQTAVINVFRGGGLQ-VNELYTLNENIRHLLKSDLGPFITDYFQNQLLAKGLTYMEEKVKLYEGENRIAILSELWDHFFTEILPTLQAIFYPVQGQELTIRQIALLGFRDSVVLKVNLGKAL--SMASSQLPPSILQMLLVLQSVHEPLGP-NESYLRLEELVKMVVSPYVGAYG-------DRHCSNMAASGIHESRMSRSHPRAEAMP 286
[Close]
BLAST of SMED30009612 vs. Ensembl Xenopus
Match: PRR5L (uncharacterized loc100145110 [Source:Xenbase;Acc:XB-GENE-5780652])

HSP 1 Score: 49.6766 bits (117), Expect = 4.446e-6
Identity = 49/215 (22.79%), Postives = 88/215 (40.93%), Query Frame = 3 
Query:  294 WQKVQEALTDSLLGKSIK-----NLSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRIT--NARN----LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIFVDGRLIHKCKNVKIYQNHFDSDSKKQMKTKRIP 905
            W  VQ A+ +   G  ++      L+E    IR L +      I DY++NQ+L   L  ++ ++      N    L E W  FF + LP +  I   ++  +  + ++ L  F+  +    +L K L   + SS     +  M LVLQ   +      +  +  EE+  ++++P VG  G          C N+     H    S+   + + +P
Sbjct:  184 WNSVQTAVINVFRGGGLQVNELYTLNEN---IRHLLKSDLGPFITDYFQNQLLAKGLTYMEEKVKLYEGENRIAILSELWDHFFTEILPTLQAIFYPVQGQELTIRQIALLGFRDSVVLKVNLGKAL--SMASSQLPPSILQMLLVLQSVHEPLGP-NESYLRLEELVKMVVSPYVGAYG-------DRHCSNMAASGIHESRMSRSHPRAEAMP 385
[Close]
BLAST of SMED30009612 vs. Ensembl Mouse
Match: Prr5l (proline rich 5 like [Source:MGI Symbol;Acc:MGI:1919696])

HSP 1 Score: 52.7582 bits (125), Expect = 3.019e-7
Identity = 44/182 (24.18%), Postives = 86/182 (47.25%), Query Frame = 3 
Query:  279 SKNENWQKVQEALTDSLLGKSIKN--LSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSG 800
            S N  W  VQ A+ +   G  +++  L  +   IR+L +      I DY++NQ+L   L  ++ +I           L E W  FF ++LP +  I   ++  +  + +++L  F R L  ++     ++   +S   SSV++ M L+LQ   +     ++  ++ EE+   +++P +  SG
Sbjct:   48 SSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGF-RDLVLLKVKLGDVLLLAQSKLPSSVIQ-MLLILQSVHEPTGP-SEGYLQLEELVKQVVSPFLSISG 226
[Close]
BLAST of SMED30009612 vs. Ensembl Mouse
Match: Prr5l (proline rich 5 like [Source:MGI Symbol;Acc:MGI:1919696])

HSP 1 Score: 52.7582 bits (125), Expect = 3.019e-7
Identity = 44/182 (24.18%), Postives = 86/182 (47.25%), Query Frame = 3 
Query:  279 SKNENWQKVQEALTDSLLGKSIKN--LSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSG 800
            S N  W  VQ A+ +   G  +++  L  +   IR+L +      I DY++NQ+L   L  ++ +I           L E W  FF ++LP +  I   ++  +  + +++L  F R L  ++     ++   +S   SSV++ M L+LQ   +     ++  ++ EE+   +++P +  SG
Sbjct:   48 SSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGF-RDLVLLKVKLGDVLLLAQSKLPSSVIQ-MLLILQSVHEPTGP-SEGYLQLEELVKQVVSPFLSISG 226
[Close]
BLAST of SMED30009612 vs. Ensembl Mouse
Match: Prr5l (proline rich 5 like [Source:MGI Symbol;Acc:MGI:1919696])

HSP 1 Score: 52.7582 bits (125), Expect = 3.019e-7
Identity = 44/182 (24.18%), Postives = 86/182 (47.25%), Query Frame = 3 
Query:  279 SKNENWQKVQEALTDSLLGKSIKN--LSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSG 800
            S N  W  VQ A+ +   G  +++  L  +   IR+L +      I DY++NQ+L   L  ++ +I           L E W  FF ++LP +  I   ++  +  + +++L  F R L  ++     ++   +S   SSV++ M L+LQ   +     ++  ++ EE+   +++P +  SG
Sbjct:   48 SSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGF-RDLVLLKVKLGDVLLLAQSKLPSSVIQ-MLLILQSVHEPTGP-SEGYLQLEELVKQVVSPFLSISG 226
[Close]
BLAST of SMED30009612 vs. UniProt/SwissProt
Match: sp|A1L1K1|PRR5L_RAT (Proline-rich protein 5-like OS=Rattus norvegicus OX=10116 GN=Prr5l PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.971e-8
Identity = 46/182 (25.27%), Postives = 87/182 (47.80%), Query Frame = 3 
Query:  279 SKNENWQKVQEALTDSLLGKSIKN--LSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSG 800
            S N  W  VQ A+ +   G  +++  L  +   IR+L +      I DY++NQ+L   L  ++ +I           L E W  FF ++LP +  I   ++  +  + +++L  F R L  ++     L+   +S   SSV++ M L+LQ   +     ++  ++ EE+   +++P +G SG
Sbjct:   48 SSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKQCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGF-RDLVLLKVKLGDLLLLSQSKLPSSVIQ-MLLILQSVHEPTGP-SEGYLQLEELVKQVVSPFLGLSG 226
[Close]
BLAST of SMED30009612 vs. UniProt/SwissProt
Match: sp|A2AVJ5|PRR5L_MOUSE (Proline-rich protein 5-like OS=Mus musculus OX=10090 GN=Prr5l PE=1 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 2.112e-6
Identity = 44/182 (24.18%), Postives = 86/182 (47.25%), Query Frame = 3 
Query:  279 SKNENWQKVQEALTDSLLGKSIKN--LSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSG 800
            S N  W  VQ A+ +   G  +++  L  +   IR+L +      I DY++NQ+L   L  ++ +I           L E W  FF ++LP +  I   ++  +  + +++L  F R L  ++     ++   +S   SSV++ M L+LQ   +     ++  ++ EE+   +++P +  SG
Sbjct:   48 SSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLLKSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGF-RDLVLLKVKLGDVLLLAQSKLPSSVIQ-MLLILQSVHEPTGP-SEGYLQLEELVKQVVSPFLSISG 226
[Close]
BLAST of SMED30009612 vs. UniProt/SwissProt
Match: sp|Q6MZQ0|PRR5L_HUMAN (Proline-rich protein 5-like OS=Homo sapiens OX=9606 GN=PRR5L PE=1 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 2.197e-6
Identity = 44/183 (24.04%), Postives = 88/183 (48.09%), Query Frame = 3 
Query:  279 SKNENWQKVQEALTDSLLGKSIKNLSEVLCC---IRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSG 800
            S +  W  VQ A+ +   G  +++ +E+      IR+L +      I DY++NQ+L   L  ++ +I           L E W  FF ++LP +  I   ++  +  + +++L  F R L  ++     L+   +S   SS+++ M L+LQ   +     ++  ++ EE+   +++P +G SG
Sbjct:   48 SSSSAWNSVQTAVINVFKGGGLQS-NELYALNENIRRLLKSELGSFITDYFQNQLLAKGLFFVEEKIKLCEGENRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIRQISLLGF-RDLVLLKVKLGDLLLLAQSKLPSSIVQ-MLLILQSVHEPTGP-SESYLQLEELVKQVVSPFLGISG 226
[Close]
BLAST of SMED30009612 vs. TrEMBL
Match: A0A1I8GSD2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028771g2 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.926e-10
Identity = 49/191 (25.65%), Postives = 89/191 (46.60%), Query Frame = 3 
Query:  267 EIFSSKNENWQKVQEALTDSLLGKSIKNLSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNA--------RNLEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSV---LKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIF 806
            E  S++N  W +VQ AL D+  G+S  NL      +R L +  ++ ++ DY E  +LK   + + RR+  +         ++E +W  F  + +  V  IL  +    + +ER  +  F+ ++     L   L   +  S   S+   L  M LVLQ   +D    ++ DV  E I ++ + P +G+ G++
Sbjct:   59 EGLSARNPVWMEVQTALIDAFKGRSNPNLGHTARLVRLLSKTVSTGLVFDYLEKSLLKKGFKIICRRLDCSSQDPEELLESMEAAWRFFHDQVVTEVKCILQPMGLDLQLLERALMLGFRDQVLLPTGLHGILRSEVACSPVCSLSPGLTRMLLVLQ-MHRDSEPPSENDVALERIVALTVRPYIGFLGVY 248
[Close]
BLAST of SMED30009612 vs. TrEMBL
Match: A0A1I8HDY9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028771g3 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.452e-10
Identity = 49/191 (25.65%), Postives = 89/191 (46.60%), Query Frame = 3 
Query:  267 EIFSSKNENWQKVQEALTDSLLGKSIKNLSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNA--------RNLEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSV---LKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIF 806
            E  S++N  W +VQ AL D+  G+S  NL      +R L +  ++ ++ DY E  +LK   + + RR+  +         ++E +W  F  + +  V  IL  +    + +ER  +  F+ ++     L   L   +  S   S+   L  M LVLQ   +D    ++ DV  E I ++ + P +G+ G++
Sbjct:   59 EGLSARNPVWMEVQTALIDAFKGRSNPNLGHTARLVRLLSKTVSTGLVFDYLEKSLLKKGFKIICRRLDCSSQDPEELLESMEAAWRFFHDQVVTEVKCILQPMGLDLQLLERALMLGFRDQVLLPTGLHGILRSEVACSPVCSLSPGLTRMLLVLQ-MHRDSEPPSENDVALERIVALTVRPYIGFLGVY 248
[Close]
BLAST of SMED30009612 vs. TrEMBL
Match: A0A267FEI8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028771g1 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.974e-10
Identity = 49/191 (25.65%), Postives = 89/191 (46.60%), Query Frame = 3 
Query:  267 EIFSSKNENWQKVQEALTDSLLGKSIKNLSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNA--------RNLEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSV---LKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIF 806
            E  S++N  W +VQ AL D+  G+S  NL      +R L +  ++ ++ DY E  +LK   + + RR+  +         ++E +W  F  + +  V  IL  +    + +ER  +  F+ ++     L   L   +  S   S+   L  M LVLQ   +D    ++ DV  E I ++ + P +G+ G++
Sbjct:   59 EGLSARNPVWMEVQTALIDAFKGRSNPNLGHTARLVRLLSKTVSTGLVFDYLEKSLLKKGFKIICRRLDCSSQDPEELLESMEAAWRFFHDQVVTEVKCILQPMGLDLQLLERALMLGFRDQVLLPTGLHGILRSEVACSPVCSLSPGLTRMLLVLQ-MHRDSEPPSENDVALERIVALTVRPYIGFLGVY 248
[Close]
BLAST of SMED30009612 vs. TrEMBL
Match: V4A385 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_210398 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.599e-8
Identity = 56/210 (26.67%), Postives = 100/210 (47.62%), Query Frame = 3 
Query:  213 RTYTEKTMSQSTTSLDQIEIFSSKNENWQKVQEALTDSLLGKSIKN--LSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKR----RLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIF 806
            R  T  T   + T+ D     S++ E  + +Q A+      K ++   L ++   +R L +  A  +I DYY++Q+LK  +  L+ R+ N R       L ++W  FF++ LP +  IL  LK  +  +  VTL EF+     +++  + LR        ++     +K M LVLQG  +D    T+     E++ ++ I P +G  G++
Sbjct:   33 RGSTLPTFLPALTTSDA----SNRQEQLESIQTAIIHLFQNKGLRENELGKLQENVRYLSDTEAGPLIFDYYKDQLLKKGMVILRERLKNERGADLLRLLGDTWDGFFKEMLPYLQAILLPLKNIERSIRDVTLLEFRNIVLLKVSSYDALRT-------ATFIPPNIKQMLLVLQGV-QDCGFSTENAFNLEKLVAMAIVPYLGIRGLY 230
[Close]
BLAST of SMED30009612 vs. TrEMBL
Match: A0A1S3HFV2 (proline-rich protein 5 OS=Lingula unguis OX=7574 GN=LOC106154922 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.417e-8
Identity = 55/206 (26.70%), Postives = 95/206 (46.12%), Query Frame = 3 
Query:  222 TEKTMSQSTTSLDQIEIFSSKNENWQKVQEALTDSLLGKSIKNL--SEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARN------LEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIFVDG 815
            T    S + +SL   E F S+NE W +VQ A+      K I  L   E+L  +R L +      I ++Y +Q+LK  +  L+ +I + R+      L E W +FFR+ LP +  IL  ++T +  + +VTL  F+  +    +    +    E       +  M LVLQ   ++    T+   + E + + ++ P +G  G +  G
Sbjct:   31 TPPPRSPTRSSLPH-EGFGSRNEYWNRVQSAIVTVFQQKRIDPLELPEILEIVRDLVKSDVGPFIYEFYRDQLLKKGMVILREKIKHYRDKELLYHLGELWNKFFREILPLIQAILQPIQTKELSIRQVTLLGFRDIVLLKTEFEACVGAGFEI---PPAVTQMLLVLQ-RLRESLPPTENFFKVESLLAQIVDPYLGVRGYYEGG 231
[Close]
BLAST of SMED30009612 vs. Planmine SMEST
Match: SMESG000061853.1 (SMESG000061853.1)

HSP 1 Score: 611.298 bits (1575), Expect = 0.000e+0
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 3 
Query:   48 MNSEFTPADLTEKNSISNKKIEDRFKYVSSLGRAVERSHESFLMKLKKKRQIKNSRTYTEKTMSQSTTSLDQIEIFSSKNENWQKVQEALTDSLLGKSIKNLSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARNLEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIFVDGRLIHKCKNVKIYQNHFDSDSKKQMKTKRIPIKGLFEKSKSLLDITGKF 959
            MNSEFTPADLTEKNSISNKKIEDRFKYVSSLGRAVERSHESFLMKLKKKRQIKNSRTYTEKTMSQSTTSLDQIEIFSSKNENWQKVQEALTDSLLGKSIKNLSEVLCCIRQLDEQYAS IIVDYYENQILKNTLQKLQRRITNARNLEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVL+NMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIFVDGRLIHKCKNVKIYQNHFDSDSKKQMKTKRIPIKGLFEKSKSLLDITGKF
Sbjct:    1 MNSEFTPADLTEKNSISNKKIEDRFKYVSSLGRAVERSHESFLMKLKKKRQIKNSRTYTEKTMSQSTTSLDQIEIFSSKNENWQKVQEALTDSLLGKSIKNLSEVLCCIRQLDEQYASNIIVDYYENQILKNTLQKLQRRITNARNLEESWVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIKHLESSTDSSVLQNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYSGIFVDGRLIHKCKNVKIYQNHFDSDSKKQMKTKRIPIKGLFEKSKSLLDITGKF 304
The following BLAST results are available for this feature:
BLAST of SMED30009612 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
PRR5L4.576e-724.04proline rich 5 like [Source:HGNC Symbol;Acc:HGNC:2... [more]
PRR5L4.576e-724.04proline rich 5 like [Source:HGNC Symbol;Acc:HGNC:2... [more]
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BLAST of SMED30009612 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009612 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009612 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009612 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
PRR5L3.477e-622.54uncharacterized loc100145110 [Source:Xenbase;Acc:X... [more]
PRR5L4.446e-622.79uncharacterized loc100145110 [Source:Xenbase;Acc:X... [more]
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BLAST of SMED30009612 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Prr5l3.019e-724.18proline rich 5 like [Source:MGI Symbol;Acc:MGI:191... [more]
Prr5l3.019e-724.18proline rich 5 like [Source:MGI Symbol;Acc:MGI:191... [more]
Prr5l3.019e-724.18proline rich 5 like [Source:MGI Symbol;Acc:MGI:191... [more]
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BLAST of SMED30009612 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|A1L1K1|PRR5L_RAT4.971e-825.27Proline-rich protein 5-like OS=Rattus norvegicus O... [more]
sp|A2AVJ5|PRR5L_MOUSE2.112e-624.18Proline-rich protein 5-like OS=Mus musculus OX=100... [more]
sp|Q6MZQ0|PRR5L_HUMAN2.197e-624.04Proline-rich protein 5-like OS=Homo sapiens OX=960... [more]
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BLAST of SMED30009612 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8GSD22.926e-1025.65Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HDY93.452e-1025.65Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FEI83.974e-1025.65Uncharacterized protein OS=Macrostomum lignano OX=... [more]
V4A3851.599e-826.67Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A1S3HFV23.417e-826.70proline-rich protein 5 OS=Lingula unguis OX=7574 G... [more]
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BLAST of SMED30009612 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009612 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009612 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009612 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009612 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009612 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000061853.10.000e+099.34SMESG000061853.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009612 ID=SMED30009612|Name=SMED30009612|organism=Schmidtea mediterranea sexual|type=transcript|length=1050bp
CTATATAAATCTTATATGAGTAACTGACAAAGTGAACAGGCAGAATTATG
AATTCGGAATTTACTCCAGCTGATTTAACAGAAAAAAATTCAATTTCTAA
TAAAAAGATCGAAGACCGTTTCAAGTATGTTAGCAGTCTTGGTCGAGCTG
TTGAAAGGTCTCACGAAAGTTTCCTGATGAAGTTGAAAAAGAAGCGGCAA
ATCAAAAACTCAAGAACCTACACTGAAAAAACAATGAGTCAATCAACGAC
TTCTTTGGATCAAATTGAAATTTTCTCTTCTAAAAATGAAAATTGGCAAA
AAGTTCAAGAAGCTTTGACTGATTCCTTATTGGGGAAATCCATAAAGAAC
CTAAGTGAAGTCCTTTGCTGTATCAGGCAATTGGATGAACAGTACGCCTC
TAAGATTATCGTTGATTACTATGAGAATCAAATACTTAAAAATACTCTAC
AAAAATTACAAAGACGCATTACAAACGCGCGAAACCTAGAAGAAAGTTGG
GTTCAGTTTTTTAGGAAATCATTGCCCAATGTCATTCTTATTTTATCATC
ATTAAAAACACATCAAGAGTATGTCGAAAGGGTTACCCTTTATGAATTCA
AAAGAAGACTAACAAAAGTTGAAGATTTGAGAAAGAAATTAATTAAACAT
CTAGAAAGTTCTACGGATTCCTCGGTACTCAAAAACATGTTTTTGGTTCT
ACAAGGCACGAAAAAAGACGATAATGAGATCACAGACATTGATGTTGAGT
TTGAAGAAATAACGTCCGTATTAATCACGCCATTGGTTGGTTACAGTGGG
ATTTTTGTCGACGGTCGGTTAATTCACAAGTGCAAAAATGTTAAAATTTA
TCAAAATCATTTTGATTCTGACTCAAAAAAACAAATGAAGACAAAAAGAA
TACCCATTAAAGGACTGTTTGAAAAATCTAAAAGTCTTTTGGATATCACT
GGGAAGTTTTAAAATCAACCCTTGAGTTGTCACATCAAAGACCTTCAAAA
GTTTTTTGATGTCTTTCAAATCTTTTGTTGAGTGTATTAAAGATATTTTG
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protein sequence of SMED30009612-orf-1

>SMED30009612-orf-1 ID=SMED30009612-orf-1|Name=SMED30009612-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=305bp
MNSEFTPADLTEKNSISNKKIEDRFKYVSSLGRAVERSHESFLMKLKKKR
QIKNSRTYTEKTMSQSTTSLDQIEIFSSKNENWQKVQEALTDSLLGKSIK
NLSEVLCCIRQLDEQYASKIIVDYYENQILKNTLQKLQRRITNARNLEES
WVQFFRKSLPNVILILSSLKTHQEYVERVTLYEFKRRLTKVEDLRKKLIK
HLESSTDSSVLKNMFLVLQGTKKDDNEITDIDVEFEEITSVLITPLVGYS
GIFVDGRLIHKCKNVKIYQNHFDSDSKKQMKTKRIPIKGLFEKSKSLLDI
TGKF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000421tail
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 127..147