SMED30009602
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009602 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30009602 aligns in the following genomic locations:
Homology
BLAST of SMED30009602 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 416.772 bits (1070), Expect = 5.697e-133 Identity = 255/285 (89.47%), Postives = 260/285 (91.23%), Query Frame = 3 Query: 45 MMENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKIEKSNKKETTKKQIIPEVVGXXXXXXXXXXXXFHEDSIKNRLRARNTSTINRLSNSNSSIDLPVIEHANPEKRKCQNSQSGDSIQDFFSHEIFKGGAALPNKTAQISXXXXXXXXXXXXXXXXXXESILENGSIDKSIINNYYIDLHKPIDKINADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIP------EKPISNRFIENQNQSNIDRSILINK 881 MMENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKI K NKKETTKKQIIPEVVGKKKCKQKLNIIKFHEDSIKNRLRARNTSTINRLSNSNSSIDL VIEHAN EKRKCQN+QSGDSIQDFFSHEI KGGAALPNKTAQISKKKKRKMILKK+K+TKNTESILEN SIDKSIINNYYIDLH KINADKSIINENMPSKL P NSSSID QH+SNRSP DNSSVIP EK ISN FIEN+NQSNI S LINK Sbjct: 1 MMENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKILKPNKKETTKKQIIPEVVGKKKCKQKLNIIKFHEDSIKNRLRARNTSTINRLSNSNSSIDLSVIEHANLEKRKCQNNQSGDSIQDFFSHEISKGGAALPNKTAQISKKKKRKMILKKSKVTKNTESILENCSIDKSIINNYYIDLH----KINADKSIINENMPSKLHPINSSSIDIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINK 281 HSP 2 Score: 111.309 bits (277), Expect = 6.889e-27 Identity = 61/82 (74.39%), Postives = 67/82 (81.71%), Query Frame = 3 Query: 687 ADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGSNTDESGL 932 A+KSIINEN S L +SS I+ ++ +S DNSSVIPEKPISNRFIENQNQSNIDRSILINKL LPIECLGSN DESGL Sbjct: 824 ANKSIINENQ-SNLSSIDSSGIEILALTEQSQVDNSSVIPEKPISNRFIENQNQSNIDRSILINKLRLPIECLGSNNDESGL 904 HSP 3 Score: 85.1149 bits (209), Expect = 5.152e-18 Identity = 51/82 (62.20%), Postives = 60/82 (73.17%), Query Frame = 3 Query: 684 NADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIP------EKPISNRFIENQNQSNIDRSILINKLLLPIECLGS 911 N+++SI++E +P KLPP NSSSID QH+SNRSP DNSSVIP EK ISN FIEN NQSNI+ S+LINK I L S Sbjct: 332 NSERSILSE-IPPKLPPINSSSIDIQHLSNRSPVDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLES 412 HSP 4 Score: 61.2326 bits (147), Expect = 4.419e-10 Identity = 42/92 (45.65%), Postives = 60/92 (65.22%), Query Frame = 3 Query: 612 ENGSIDKSIINNYYIDLHKPIDKINADKSIINE--NMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINK 881 EN S IINN + P+ +++++SI++E + S L P NSS I+ + +S +SP NS ++PEK ISN FIE +NQSNI S+LINK Sbjct: 558 ENPSKINDIINNSH-----PLINLDSERSILSEIPEIISILSPINSSGIEIETLSEQSPVKNSLLLPEKSISNEFIEYRNQSNIIDSVLINK 644 HSP 5 Score: 59.3066 bits (142), Expect = 1.751e-9 Identity = 43/77 (55.84%), Postives = 48/77 (62.34%), Query Frame = 3 Query: 687 ADKSIINEN--MPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGS 911 +DKSIINEN M SKL KN +SN+S DNSSV PEK ISN FIEN NQS+I S+LIN I L S Sbjct: 670 SDKSIINENSKMSSKLSHKN--------LSNKSQVDNSSVFPEKSISNEFIENLNQSDIIDSVLINNSQTSIRYLES 738 HSP 6 Score: 55.8398 bits (133), Expect = 2.442e-8 Identity = 41/88 (46.59%), Postives = 53/88 (60.23%), Query Frame = 3 Query: 675 DKINADKSIINE--NMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGSNTDESGL 932 DK AD+SIINE +PS L +SSS++ Q +S FIEN+NQSNID S++IN+ LP+EC GSNT+E GL Sbjct: 749 DKNIADQSIINEISEIPSNLSSIDSSSVEIQALS------------------EFIENRNQSNIDGSVIINEFQLPMECSGSNTNEPGL 818
BLAST of SMED30009602 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 413.69 bits (1062), Expect = 6.447e-132 Identity = 254/284 (89.44%), Postives = 259/284 (91.20%), Query Frame = 3 Query: 48 MENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKIEKSNKKETTKKQIIPEVVGXXXXXXXXXXXXFHEDSIKNRLRARNTSTINRLSNSNSSIDLPVIEHANPEKRKCQNSQSGDSIQDFFSHEIFKGGAALPNKTAQISXXXXXXXXXXXXXXXXXXESILENGSIDKSIINNYYIDLHKPIDKINADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIP------EKPISNRFIENQNQSNIDRSILINK 881 MENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKI K NKKETTKKQIIPEVVGKKKCKQKLNIIKFHEDSIKNRLRARNTSTINRLSNSNSSIDL VIEHAN EKRKCQN+QSGDSIQDFFSHEI KGGAALPNKTAQISKKKKRKMILKK+K+TKNTESILEN SIDKSIINNYYIDLH KINADKSIINENMPSKL P NSSSID QH+SNRSP DNSSVIP EK ISN FIEN+NQSNI S LINK Sbjct: 1 MENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKILKPNKKETTKKQIIPEVVGKKKCKQKLNIIKFHEDSIKNRLRARNTSTINRLSNSNSSIDLSVIEHANLEKRKCQNNQSGDSIQDFFSHEISKGGAALPNKTAQISKKKKRKMILKKSKVTKNTESILENCSIDKSIINNYYIDLH----KINADKSIINENMPSKLHPINSSSIDIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINK 280 HSP 2 Score: 111.309 bits (277), Expect = 7.937e-27 Identity = 61/82 (74.39%), Postives = 67/82 (81.71%), Query Frame = 3 Query: 687 ADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGSNTDESGL 932 A+KSIINEN S L +SS I+ ++ +S DNSSVIPEKPISNRFIENQNQSNIDRSILINKL LPIECLGSN DESGL Sbjct: 823 ANKSIINENQ-SNLSSIDSSGIEILALTEQSQVDNSSVIPEKPISNRFIENQNQSNIDRSILINKLRLPIECLGSNNDESGL 903 HSP 3 Score: 85.1149 bits (209), Expect = 6.032e-18 Identity = 51/82 (62.20%), Postives = 60/82 (73.17%), Query Frame = 3 Query: 684 NADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIP------EKPISNRFIENQNQSNIDRSILINKLLLPIECLGS 911 N+++SI++E +P KLPP NSSSID QH+SNRSP DNSSVIP EK ISN FIEN NQSNI+ S+LINK I L S Sbjct: 331 NSERSILSE-IPPKLPPINSSSIDIQHLSNRSPVDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLES 411 HSP 4 Score: 61.2326 bits (147), Expect = 4.792e-10 Identity = 42/92 (45.65%), Postives = 60/92 (65.22%), Query Frame = 3 Query: 612 ENGSIDKSIINNYYIDLHKPIDKINADKSIINE--NMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINK 881 EN S IINN + P+ +++++SI++E + S L P NSS I+ + +S +SP NS ++PEK ISN FIE +NQSNI S+LINK Sbjct: 557 ENPSKINDIINNSH-----PLINLDSERSILSEIPEIISILSPINSSGIEIETLSEQSPVKNSLLLPEKSISNEFIEYRNQSNIIDSVLINK 643 HSP 5 Score: 59.3066 bits (142), Expect = 1.899e-9 Identity = 43/77 (55.84%), Postives = 48/77 (62.34%), Query Frame = 3 Query: 687 ADKSIINEN--MPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGS 911 +DKSIINEN M SKL KN +SN+S DNSSV PEK ISN FIEN NQS+I S+LIN I L S Sbjct: 669 SDKSIINENSKMSSKLSHKN--------LSNKSQVDNSSVFPEKSISNEFIENLNQSDIIDSVLINNSQTSIRYLES 737 HSP 6 Score: 55.8398 bits (133), Expect = 2.508e-8 Identity = 41/88 (46.59%), Postives = 53/88 (60.23%), Query Frame = 3 Query: 675 DKINADKSIINE--NMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGSNTDESGL 932 DK AD+SIINE +PS L +SSS++ Q +S FIEN+NQSNID S++IN+ LP+EC GSNT+E GL Sbjct: 748 DKNIADQSIINEISEIPSNLSSIDSSSVEIQALS------------------EFIENRNQSNIDGSVIINEFQLPMECSGSNTNEPGL 817
BLAST of SMED30009602 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 407.912 bits (1047), Expect = 7.372e-130 Identity = 254/289 (87.89%), Postives = 259/289 (89.62%), Query Frame = 3 Query: 48 MENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKIEKSNKKETTKKQIIPEVVGXXXXXXXXXXXXFHEDSIKNRLRARNTSTINRLSNSNSSIDLPVIEHANPE-----KRKCQNSQSGDSIQDFFSHEIFKGGAALPNKTAQISXXXXXXXXXXXXXXXXXXESILENGSIDKSIINNYYIDLHKPIDKINADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIP------EKPISNRFIENQNQSNIDRSILINK 881 MENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKI K NKKETTKKQIIPEVVGKKKCKQKLNIIKFHEDSIKNRLRARNTSTINRLSNSNSSIDL VIEHAN E KRKCQN+QSGDSIQDFFSHEI KGGAALPNKTAQISKKKKRKMILKK+K+TKNTESILEN SIDKSIINNYYIDLH KINADKSIINENMPSKL P NSSSID QH+SNRSP DNSSVIP EK ISN FIEN+NQSNI S LINK Sbjct: 1 MENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSFKSPKADKNKILKPNKKETTKKQIIPEVVGKKKCKQKLNIIKFHEDSIKNRLRARNTSTINRLSNSNSSIDLSVIEHANLEVKPQRKRKCQNNQSGDSIQDFFSHEISKGGAALPNKTAQISKKKKRKMILKKSKVTKNTESILENCSIDKSIINNYYIDLH----KINADKSIINENMPSKLHPINSSSIDIQHLSNRSPVDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINK 285 HSP 2 Score: 111.309 bits (277), Expect = 7.362e-27 Identity = 61/82 (74.39%), Postives = 67/82 (81.71%), Query Frame = 3 Query: 687 ADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGSNTDESGL 932 A+KSIINEN S L +SS I+ ++ +S DNSSVIPEKPISNRFIENQNQSNIDRSILINKL LPIECLGSN DESGL Sbjct: 828 ANKSIINENQ-SNLSSIDSSGIEILALTEQSQVDNSSVIPEKPISNRFIENQNQSNIDRSILINKLRLPIECLGSNNDESGL 908 HSP 3 Score: 85.1149 bits (209), Expect = 5.448e-18 Identity = 51/82 (62.20%), Postives = 60/82 (73.17%), Query Frame = 3 Query: 684 NADKSIINENMPSKLPPKNSSSIDFQHISNRSPADNSSVIP------EKPISNRFIENQNQSNIDRSILINKLLLPIECLGS 911 N+++SI++E +P KLPP NSSSID QH+SNRSP DNSSVIP EK ISN FIEN NQSNI+ S+LINK I L S Sbjct: 336 NSERSILSE-IPPKLPPINSSSIDIQHLSNRSPVDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLES 416 HSP 4 Score: 61.2326 bits (147), Expect = 4.500e-10 Identity = 42/92 (45.65%), Postives = 60/92 (65.22%), Query Frame = 3 Query: 612 ENGSIDKSIINNYYIDLHKPIDKINADKSIINE--NMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINK 881 EN S IINN + P+ +++++SI++E + S L P NSS I+ + +S +SP NS ++PEK ISN FIE +NQSNI S+LINK Sbjct: 562 ENPSKINDIINNSH-----PLINLDSERSILSEIPEIISILSPINSSGIEIETLSEQSPVKNSLLLPEKSISNEFIEYRNQSNIIDSVLINK 648 HSP 5 Score: 59.3066 bits (142), Expect = 1.784e-9 Identity = 43/77 (55.84%), Postives = 48/77 (62.34%), Query Frame = 3 Query: 687 ADKSIINEN--MPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGS 911 +DKSIINEN M SKL KN +SN+S DNSSV PEK ISN FIEN NQS+I S+LIN I L S Sbjct: 674 SDKSIINENSKMSSKLSHKN--------LSNKSQVDNSSVFPEKSISNEFIENLNQSDIIDSVLINNSQTSIRYLES 742 HSP 6 Score: 55.8398 bits (133), Expect = 2.487e-8 Identity = 41/88 (46.59%), Postives = 53/88 (60.23%), Query Frame = 3 Query: 675 DKINADKSIINE--NMPSKLPPKNSSSIDFQHISNRSPADNSSVIPEKPISNRFIENQNQSNIDRSILINKLLLPIECLGSNTDESGL 932 DK AD+SIINE +PS L +SSS++ Q +S FIEN+NQSNID S++IN+ LP+EC GSNT+E GL Sbjct: 753 DKNIADQSIINEISEIPSNLSSIDSSSVEIQALS------------------EFIENRNQSNIDGSVIINEFQLPMECSGSNTNEPGL 822 The following BLAST results are available for this feature:
BLAST of SMED30009602 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30009602 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30009602 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30009602 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30009602 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009602 ID=SMED30009602|Name=SMED30009602|organism=Schmidtea mediterranea sexual|type=transcript|length=934bpback to top protein sequence of SMED30009602-orf-1 >SMED30009602-orf-1 ID=SMED30009602-orf-1|Name=SMED30009602-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=296bp MMENTLEFSLDSSVDIDQYDWSTQITNYSDLTFNNSTAKKFSKKNVTNFSback to top Annotated Terms
The following terms have been associated with this transcript:
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