SMED30009586
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009586 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 27Note: Hover over icons to view figure legend
Alignments
SMED30009586 aligns in the following genomic locations:
Homology
BLAST of SMED30009586 vs. TrEMBL
Match: G7YV67 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_111660 PE=4 SV=1) HSP 1 Score: 70.8626 bits (172), Expect = 6.887e-11 Identity = 55/178 (30.90%), Postives = 82/178 (46.07%), Query Frame = 2 Query: 188 YWLDGGH-KGGIWGHSDDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRXXXXXXXXXXXXXXXXXXAVWGDFVSHNAEDKKK----------KLKGN------------------SIFSNSYQKKYHDKIWHLGWGFWTSVAADVFLFIANLVYLVERDCKTNSN 634 YW+ + K H++ Y + EY + G+L S AF L+R+ + S+ CR+L L ++F+ +L SVA W D++ H+ K LK N S+F Q H LGW +W SVA+ +FLF ANL+YL+ +DCKT+ N Sbjct: 53 YWVSNDYSKAAEDAHTNFYDSAEYYHAKLLMRCGILASFCAFVVGLLRILVPKAKNSRRCRILQFGSLVTAFVSILASVAAWADYLRHDDLTKSAGFERQHFSIWPLKPNAWSANESFRIGEIRKAAQSLFETHAQ---HSPTILLGWAYWFSVASVLFLFFANLLYLMRKDCKTDQN 227
BLAST of SMED30009586 vs. TrEMBL
Match: A0A419QDM2 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_7150s PE=4 SV=1) HSP 1 Score: 70.8626 bits (172), Expect = 7.097e-11 Identity = 55/178 (30.90%), Postives = 82/178 (46.07%), Query Frame = 2 Query: 188 YWLDGGH-KGGIWGHSDDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRXXXXXXXXXXXXXXXXXXAVWGDFVSHNAEDKKK----------KLKGN------------------SIFSNSYQKKYHDKIWHLGWGFWTSVAADVFLFIANLVYLVERDCKTNSN 634 YW+ + K H++ Y + EY + G+L S AF L+R+ + S+ CR+L L ++F+ +L SVA W D++ H+ K LK N S+F Q H LGW +W SVA+ +FLF ANL+YL+ +DCKT+ N Sbjct: 53 YWVSNDYSKAAEDAHTNFYDSAEYYHAKLLMRCGILASFCAFVVGLLRILVPKAKNSRRCRILQFGSLVTAFVSILASVAAWADYLRHDDLTKSAGFERQHFSIWPLKPNAWSANESFRIGEIRKAAQSLFETHAQ---HSPTILLGWAYWFSVASVLFLFFANLLYLMRKDCKTDQN 227
BLAST of SMED30009586 vs. Planmine SMEST
Match: SMESG000030829.1 (SMESG000030829.1) HSP 1 Score: 205.682 bits (522), Expect = 2.648e-67 Identity = 147/189 (77.78%), Postives = 147/189 (77.78%), Query Frame = 2 Query: 122 VVLVFXXXXXXXXXXXXXXXXNYWLDGGHKGGIWGHSDDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRXXXXXXXXXXXXXXXXXXAVWGDFVSHNAEDKKKKLKGNSIFSNSYQKKYHDKIWHLGWGFWTSVAADVFLFIANLVYLVERDCKTNSNEGIAIEENPNDRGQSDLI 688 VVLVFICGIACGLAIIGVAGGNYWLDGGHKGGIWGHSDDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRLLFVSFLGSSFLCLLVSVAVWGDFVSHNAEDKKKKLK VERDCKTNSNEGIAIEENPNDRGQSDLI Sbjct: 12 VVLVFICGIACGLAIIGVAGGNYWLDGGHKGGIWGHSDDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRLLFVSFLGSSFLCLLVSVAVWGDFVSHNAEDKKKKLK------------------------------------------VERDCKTNSNEGIAIEENPNDRGQSDLI 158
BLAST of SMED30009586 vs. Planmine SMEST
Match: SMESG000030829.1 (SMESG000030829.1) HSP 1 Score: 202.986 bits (515), Expect = 2.297e-66 Identity = 146/189 (77.25%), Postives = 146/189 (77.25%), Query Frame = 2 Query: 122 VVLVFXXXXXXXXXXXXXXXXNYWLDGGHKGGIWGHSDDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRXXXXXXXXXXXXXXXXXXAVWGDFVSHNAEDKKKKLKGNSIFSNSYQKKYHDKIWHLGWGFWTSVAADVFLFIANLVYLVERDCKTNSNEGIAIEENPNDRGQSDLI 688 VVLVFICGIACGLAIIGVAGGNYWLDGGHKGGIWGHSDDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRLLFVSFLGSSFLCLLVSVAVWGDFVSHNAEDKKKKLK ERDCKTNSNEGIAIEENPNDRGQSDLI Sbjct: 12 VVLVFICGIACGLAIIGVAGGNYWLDGGHKGGIWGHSDDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRLLFVSFLGSSFLCLLVSVAVWGDFVSHNAEDKKKKLK-------------------------------------------ERDCKTNSNEGIAIEENPNDRGQSDLI 157
BLAST of SMED30009586 vs. Planmine SMEST
Match: SMESG000023295.1 (SMESG000023295.1) HSP 1 Score: 71.2478 bits (173), Expect = 9.525e-15 Identity = 47/150 (31.33%), Postives = 85/150 (56.67%), Query Frame = 2 Query: 203 GHKGGIWGHS-DDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRXXXXXXXXXXXXXXXXXXAVWGDFVSHNAE-DKKKKLKGN----------SIFSNSYQKKYHDKIWHLGWGFWTSVAADVFLFIANLVYLVERD 616 + GIW + ++ EY +TR F++ GML S F S+ ++KF++ + +++ +++ L SF+ +L+++ +WGD V H E KKK N +FS S Q K W++GW +W +VA ++ LF+ANL++ +++D Sbjct: 49 SEESGIWNFNIHEFDLDEYNSTRFFAIFGMLSSTSVFILSICQIKFKQINENQTADHIYIILLTLSFISILLALWIWGDLVRHLKEVGLKKKNPFNKKDTYIYELYELFSKSTQDKSLANKWNIGWSYWLTVAGELNLFVANLLFFMKKD 198
BLAST of SMED30009586 vs. Planmine SMEST
Match: SMESG000023295.1 (SMESG000023295.1) HSP 1 Score: 70.8626 bits (172), Expect = 1.374e-14 Identity = 47/150 (31.33%), Postives = 85/150 (56.67%), Query Frame = 2 Query: 203 GHKGGIWGHS-DDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRXXXXXXXXXXXXXXXXXXAVWGDFVSHNAE-DKKKKLKGN----------SIFSNSYQKKYHDKIWHLGWGFWTSVAADVFLFIANLVYLVERD 616 + GIW + ++ EY +TR F++ GML S F S+ ++KF++ + +++ +++ L SF+ +L+++ +WGD V H E KKK N +FS S Q K W++GW +W +VA ++ LF+ANL++ +++D Sbjct: 63 SEESGIWNFNIHEFDLDEYNSTRFFAIFGMLSSTSVFILSICQIKFKQINENQTADHIYIILLTLSFISILLALWIWGDLVRHLKEVGLKKKNPFNKKDTYIYELYELFSKSTQDKSLANKWNIGWSYWLTVAGELNLFVANLLFFMKKD 212
BLAST of SMED30009586 vs. Planmine SMEST
Match: SMESG000023295.1 (SMESG000023295.1) HSP 1 Score: 67.0106 bits (162), Expect = 3.165e-13 Identity = 46/153 (30.07%), Postives = 83/153 (54.25%), Query Frame = 2 Query: 203 GHKGGIWGHS-DDYRNQEYQTTRAFSMIGMLISVLAFCFSLIRLKFRRTSRSKSCRXXXXXXXXXXXXXXXXXXA---VWGDFVSHNAE-DKKKKLKGN----------SIFSNSYQKKYHDKIWHLGWGFWTSVAADVFLFIANLVYLVERD 616 + GIW + ++ EY +TR F++ GML S F S+ ++KF++ + +++ +++ L SF+ ++ + +WGD V H E KKK N +FS S Q K W++GW +W +VA ++ LF+ANL++ +++D Sbjct: 49 SEESGIWNFNIHEFDLDEYNSTRFFAIFGMLSSTSVFILSICQIKFKQINENQTADHIYIILLTLSFILVISILLALWIWGDLVRHLKEVGLKKKNPFNKKDTYIYELYELFSKSTQDKSLANKWNIGWSYWLTVAGELNLFVANLLFFMKKD 201 The following BLAST results are available for this feature:
BLAST of SMED30009586 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30009586 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30009586 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 2
BLAST of SMED30009586 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30009586 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30009586 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009586 ID=SMED30009586|Name=SMED30009586|organism=Schmidtea mediterranea sexual|type=transcript|length=739bpback to top protein sequence of SMED30009586-orf-1 >SMED30009586-orf-1 ID=SMED30009586-orf-1|Name=SMED30009586-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=201bp MTKEKTCDTTDVVLVFICGIACGLAIIGVAGGNYWLDGGHKGGIWGHSDDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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