SMED30009566
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009566 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Alignments
SMED30009566 aligns in the following genomic locations:
Homology
BLAST of SMED30009566 vs. Ensembl Human
Match: TSFM (Ts translation elongation factor, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12367]) HSP 1 Score: 77.411 bits (189), Expect = 9.157e-26 Identity = 41/109 (37.61%), Postives = 56/109 (51.38%), Query Frame = -2 Query: 1435 LSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD 1761 L KLR+ +G+ CK+AL C ++ ++ WL EA K GW K KL + GLIG+ N V++E+NCETDFV+RN F + M H L D Sbjct: 50 LMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTT----VLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKD 154 HSP 2 Score: 60.8474 bits (146), Expect = 9.157e-26 Identity = 43/153 (28.10%), Postives = 75/153 (49.02%), Query Frame = -3 Query: 915 KKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSH--LSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRF 1367 ++ +L LA+ +G LGENM +RA +K ++ Y H + P ++K GKYG ++ + + T L ED R+ + QH+VG+ P+ +G S DEP + E ++ Q +L D ++ + + + + DF RF Sbjct: 178 REGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHK----LVLGKYGALVICETS-EQKTNL-EDVGRR--------LGQHVVGMAPLSVG-SLDDEPGG--EAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRF 313
BLAST of SMED30009566 vs. Ensembl Human
Match: TSFM (Ts translation elongation factor, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12367]) HSP 1 Score: 76.6406 bits (187), Expect = 9.712e-17 Identity = 40/107 (37.38%), Postives = 55/107 (51.40%), Query Frame = -2 Query: 1435 KLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD 1755 KLR+ +G+ CK+AL C ++ ++ WL EA K GW K KL + GLIG+ N V++E+NCETDFV+RN F + M H L D Sbjct: 2 KLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTT----VLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKD 104 HSP 2 Score: 31.187 bits (69), Expect = 9.712e-17 Identity = 14/41 (34.15%), Postives = 23/41 (56.10%), Query Frame = -3 Query: 1245 KKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSH 1367 ++ +L LA+ +G LGENM +RA +K ++ Y H Sbjct: 128 REGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVH 168
BLAST of SMED30009566 vs. Ensembl Human
Match: TSFM (Ts translation elongation factor, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12367]) HSP 1 Score: 77.0258 bits (188), Expect = 7.536e-16 Identity = 41/109 (37.61%), Postives = 56/109 (51.38%), Query Frame = -2 Query: 1435 LSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD 1761 L KLR+ +G+ CK+AL C ++ ++ WL EA K GW K KL + GLIG+ N V++E+NCETDFV+RN F + M H L D Sbjct: 50 LMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTT----VLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKD 154
BLAST of SMED30009566 vs. Ensembl Human
Match: TSFM (Ts translation elongation factor, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12367]) HSP 1 Score: 76.2554 bits (186), Expect = 8.210e-16 Identity = 40/107 (37.38%), Postives = 55/107 (51.40%), Query Frame = -2 Query: 1435 KLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD 1755 KLR+ +G+ CK+AL C ++ ++ WL EA K GW K KL + GLIG+ N V++E+NCETDFV+RN F + M H L D Sbjct: 2 KLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTT----VLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKD 104
BLAST of SMED30009566 vs. Ensembl Human
Match: TSFM (Ts translation elongation factor, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12367]) HSP 1 Score: 77.0258 bits (188), Expect = 1.893e-15 Identity = 41/109 (37.61%), Postives = 56/109 (51.38%), Query Frame = -2 Query: 1435 LSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD 1761 L KLR+ +G+ CK+AL C ++ ++ WL EA K GW K KL + GLIG+ N V++E+NCETDFV+RN F + M H L D Sbjct: 50 LMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTT----VLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKD 154
BLAST of SMED30009566 vs. Ensembl Celegans
Match: tsfm-1 (Elongation factor Ts, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q20819]) HSP 1 Score: 71.633 bits (174), Expect = 2.426e-13 Identity = 39/105 (37.14%), Postives = 61/105 (58.10%), Query Frame = -2 Query: 1459 KTDLSKLRKISGFPISKCKEALVKCNSN-VDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFM 1770 K L LRK +G+ C++AL++ N +D + +WLK AAK GW K KL + SNGL+ + + S ++EL+CETDFVAR+ F L+ +++ + Sbjct: 27 KEALMALRKKTGYSYVNCRKALIQFGENDMDSAVKWLKEAAAKEGWAKAAKLGTRVTSNGLVSVVTDN----STAAVVELSCETDFVARSGAFKDLLSNISNSVL 127
BLAST of SMED30009566 vs. Ensembl Fly
Match: mEFTs (gene:FBgn0032646 transcript:FBtr0080994) HSP 1 Score: 98.2117 bits (243), Expect = 1.459e-39 Identity = 56/152 (36.84%), Postives = 83/152 (54.61%), Query Frame = -3 Query: 903 TLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1358 TL LA+ +G++GEN RRA+ KA+ D L Y+H + ++ T GKYG ++ Y+ + LD + + +ICQ IVG+ P KIG D+P E+ D+E LI+Q +L D + V + L E+ EI D+ RF CGE Sbjct: 160 TLGDHLALLIGAIGENATIRRALCFKANNDLKLVGYAHPAPTNVGTTEGITQVGKYGAIVAYRSTHPL---LDFEFHK--------SICQQIVGMKPTKIGEYDKDKPAENKDDETCLIHQEYLLDADKTVGEALQEHNCEIVDYHRFECGE 300 HSP 2 Score: 85.5001 bits (210), Expect = 1.459e-39 Identity = 43/108 (39.81%), Postives = 67/108 (62.04%), Query Frame = -2 Query: 1447 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHIC 1770 K+ L+ LRK +G+ + CK+AL K N++V + +WL +A GW K K+A++ ++GLIG+ + N+ G M+ELNCETDFVARN F + F++H+ Sbjct: 29 KSALAALRKKTGYTFANCKKALEKHNNDVGLAEKWLHEQAQTLGWSKATKVADRATAHGLIGVLIRGNR----GAMVELNCETDFVARNDTFKR--------FVDHVA 124
BLAST of SMED30009566 vs. Ensembl Zebrafish
Match: tsfm (Ts translation elongation factor, mitochondrial [Source:NCBI gene;Acc:567785]) HSP 1 Score: 88.5817 bits (218), Expect = 6.493e-31 Identity = 43/106 (40.57%), Postives = 63/106 (59.43%), Query Frame = -2 Query: 1453 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEH 1770 K L +LRK +G+ CK+AL KCN+++ ++ WL +A K GW K KL + GLIG+ ++ N VM+E+NCETDFVARN+ F + + + M H Sbjct: 35 KALLLQLRKSTGYTFVNCKKALEKCNNDITQAESWLHGQAKKEGWSKATKLEGRKAKEGLIGLMMHDN----AAVMVEVNCETDFVARNEKFQQLVKDVALSVMAH 136 HSP 2 Score: 66.2402 bits (160), Expect = 6.493e-31 Identity = 49/168 (29.17%), Postives = 71/168 (42.26%), Query Frame = -3 Query: 903 LNEYSISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGV-MLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1403 L+ +S L +L LA+ +G LGEN+ RRAV+L D H+ Y H T G+ G+ M +Y + + E + + QH++G PV +GN + D E L+ Q FL D V+ L + DF RF CGE Sbjct: 150 LSSEDMSKLNAPDGPSLADQLALTIGRLGENIAMRRAVSLSVPSDWHIGSYIH-----------GTVAGQVGIEMGRYGSLVVFQGEPKEGTY-----ALGRKLAQHVMGEAPVSLGNM---DDLSCGDSETRLLPQTFLPDPKYTVAQYLTLQDARVLDFIRFQCGE 298
BLAST of SMED30009566 vs. Ensembl Zebrafish
Match: tsfm (Ts translation elongation factor, mitochondrial [Source:NCBI gene;Acc:567785]) HSP 1 Score: 88.5817 bits (218), Expect = 6.935e-31 Identity = 43/106 (40.57%), Postives = 63/106 (59.43%), Query Frame = -2 Query: 1453 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEH 1770 K L +LRK +G+ CK+AL KCN+++ ++ WL +A K GW K KL + GLIG+ ++ N VM+E+NCETDFVARN+ F + + + M H Sbjct: 37 KALLLQLRKSTGYTFVNCKKALEKCNNDITQAESWLHEQAKKEGWSKATKLEGRKAKEGLIGLMMHDN----AAVMVEVNCETDFVARNEKFQQLVKDVALSVMAH 138 HSP 2 Score: 66.2402 bits (160), Expect = 6.935e-31 Identity = 49/168 (29.17%), Postives = 71/168 (42.26%), Query Frame = -3 Query: 903 LNEYSISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGV-MLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1403 L+ +S L +L LA+ +G LGEN+ RRAV+L D H+ Y H T G+ G+ M +Y + + E + + QH++G PV +GN + D E L+ Q FL D V+ L + DF RF CGE Sbjct: 152 LSSEDMSKLNAPDGPSLADQLALTIGRLGENIAMRRAVSLSVPSDWHIGSYIH-----------GTVAGQVGIEMGRYGSLVVFQGEPKEGTY-----ALGRKLAQHVMGEAPVSLGNM---DDLSCGDSETRLLPQTFLPDPKYTVAQYLTLQDARVLDFIRFQCGE 300
BLAST of SMED30009566 vs. Ensembl Xenopus
Match: cebpz (CCAAT enhancer binding protein zeta [Source:Xenbase;Acc:XB-GENE-853892]) HSP 1 Score: 79.337 bits (194), Expect = 1.108e-31 Identity = 54/191 (28.27%), Postives = 88/191 (46.07%), Query Frame = -3 Query: 897 VNFRILMQFNSAQKNLNEYSISML-------ILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGELA 1448 V L S Q+N++ Y L + ++ L LA+ +G LGENM +RA +K D + Y H TFGKYG ++ K D D+ + N ++ + QH+VG++P+ +G+ + + E+ E ++ Q FL + +L V L + + DF RF CGE+A Sbjct: 153 VAVSTLRHCQSHQENMSSYVKGFLSGEELLQMKAEESLLKDQLALAIGKLGENMTMKRAAWVKTPSDIFIGSYMHGILVADVPSLSNITFGKYGALVICK---DSDSNP-----KSNISEVGRRLGQHVVGMNPLSVGSLEDESSGET---ETKMLAQSFLLEPSLTVGQYLQPRGINVLDFIRFECGEVA 332 HSP 2 Score: 78.5666 bits (192), Expect = 1.108e-31 Identity = 38/103 (36.89%), Postives = 60/103 (58.25%), Query Frame = -2 Query: 1453 LSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEH 1761 L KLRK +G+ CK+AL + +++ ++ WL +A K GW K KL + + GL+G+ + N + VM+E+NCETDFVARN F + + + + H Sbjct: 62 LVKLRKRTGYSFINCKKALEQFSNDFKQAETWLHQQAQKEGWDKASKLQGRKTTEGLVGLLQDGNTS----VMVEVNCETDFVARNSKFQQLVQQVAVSTLRH 160
BLAST of SMED30009566 vs. Ensembl Mouse
Match: Tsfm (Ts translation elongation factor, mitochondrial [Source:MGI Symbol;Acc:MGI:1913649]) HSP 1 Score: 79.337 bits (194), Expect = 1.116e-27 Identity = 40/109 (36.70%), Postives = 58/109 (53.21%), Query Frame = -2 Query: 1435 LSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD 1761 L KLR+ +G+ CK+AL C ++ ++ +WL +A K GW K KL + GLIG+ N V++E+NCETDFV+RN F + + M H L D Sbjct: 49 LMKLRRKTGYSFVNCKKALETCGGDLKQAEDWLHKQAQKEGWSKAAKLHGRKTKEGLIGLLQEGN----TAVLVEVNCETDFVSRNLKFQQLVQQVALGTMAHCQNLTD 153 HSP 2 Score: 64.6994 bits (156), Expect = 1.116e-27 Identity = 41/155 (26.45%), Postives = 71/155 (45.81%), Query Frame = -3 Query: 903 KKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1367 ++ +L LA+ +G LGENM +RA +K ++ Y H + + GKYG ++ + I N + + QH+VG+ P+ +G S DEP + E ++ Q +L D ++ + + + + DF RF CGE Sbjct: 177 REGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVH--GVTQSPSLQNLVLGKYGALVICETPEQI----------ANLEEVGRRLGQHVVGMAPLSVG-SLDDEPGG--ETETRMLPQPYLLDPSITLGQYVQPQGVTVVDFVRFECGE 316
BLAST of SMED30009566 vs. Ensembl Mouse
Match: Tsfm (Ts translation elongation factor, mitochondrial [Source:MGI Symbol;Acc:MGI:1913649]) HSP 1 Score: 79.7221 bits (195), Expect = 1.376e-16 Identity = 40/109 (36.70%), Postives = 58/109 (53.21%), Query Frame = -2 Query: 1435 LSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD 1761 L KLR+ +G+ CK+AL C ++ ++ +WL +A K GW K KL + GLIG+ N V++E+NCETDFV+RN F + + M H L D Sbjct: 49 LMKLRRKTGYSFVNCKKALETCGGDLKQAEDWLHKQAQKEGWSKAAKLHGRKTKEGLIGLLQEGN----TAVLVEVNCETDFVSRNLKFQQLVQQVALGTMAHCQNLTD 153
BLAST of SMED30009566 vs. Ensembl Mouse
Match: Tsfm (Ts translation elongation factor, mitochondrial [Source:MGI Symbol;Acc:MGI:1913649]) HSP 1 Score: 53.1434 bits (126), Expect = 2.155e-7 Identity = 30/96 (31.25%), Postives = 48/96 (50.00%), Query Frame = -2 Query: 1492 LSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFV------ARNQNFH 1761 L KLR+ +G+ CK+AL C ++ ++ +WL +A K GW K KL + GLIG+ N V+L L+ + V +R ++ H Sbjct: 49 LMKLRRKTGYSFVNCKKALETCGGDLKQAEDWLHKQAQKEGWSKAAKLHGRKTKEGLIGLLQEGNTAVLVEVVLCLSPQQKDVVGVERGSRGRDLH 144
BLAST of SMED30009566 vs. UniProt/SwissProt
Match: sp|Q9VJC7|EFTS_DROME (Elongation factor Ts, mitochondrial OS=Drosophila melanogaster OX=7227 GN=mEFTs PE=2 SV=1) HSP 1 Score: 98.2117 bits (243), Expect = 1.115e-38 Identity = 56/152 (36.84%), Postives = 83/152 (54.61%), Query Frame = -3 Query: 903 TLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1358 TL LA+ +G++GEN RRA+ KA+ D L Y+H + ++ T GKYG ++ Y+ + LD + + +ICQ IVG+ P KIG D+P E+ D+E LI+Q +L D + V + L E+ EI D+ RF CGE Sbjct: 160 TLGDHLALLIGAIGENATIRRALCFKANNDLKLVGYAHPAPTNVGTTEGITQVGKYGAIVAYRSTHPL---LDFEFHK--------SICQQIVGMKPTKIGEYDKDKPAENKDDETCLIHQEYLLDADKTVGEALQEHNCEIVDYHRFECGE 300 HSP 2 Score: 85.5001 bits (210), Expect = 1.115e-38 Identity = 43/108 (39.81%), Postives = 67/108 (62.04%), Query Frame = -2 Query: 1447 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHIC 1770 K+ L+ LRK +G+ + CK+AL K N++V + +WL +A GW K K+A++ ++GLIG+ + N+ G M+ELNCETDFVARN F + F++H+ Sbjct: 29 KSALAALRKKTGYTFANCKKALEKHNNDVGLAEKWLHEQAQTLGWSKATKVADRATAHGLIGVLIRGNR----GAMVELNCETDFVARNDTFKR--------FVDHVA 124
BLAST of SMED30009566 vs. UniProt/SwissProt
Match: sp|C3YEM5|EFTS_BRAFL (Elongation factor Ts, mitochondrial OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_84221 PE=3 SV=1) HSP 1 Score: 88.9669 bits (219), Expect = 1.735e-33 Identity = 49/136 (36.03%), Postives = 77/136 (56.62%), Query Frame = -2 Query: 1378 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD-----INAIQLGSEELKRILYLDV 1770 K +LSKLRK +GF C++AL K ++++++ +WLK +A K GW K KL ++ + GL+G+ Q + M+E+NCETDFVARN F + + A + + P + A+ G EELK++ D Sbjct: 32 KANLSKLRKKTGFTFVNCRKALEKFENDLEQAEKWLKEQAQKEGWAKATKLQDRQTAQGLVGV----AQEGTMATMVEVNCETDFVARNPKFRQLVTQVAMATLGDVKAHPQWTLGWLKALHTG-EELKQLQIGDT 162 HSP 2 Score: 77.411 bits (189), Expect = 1.735e-33 Identity = 46/173 (26.59%), Postives = 80/173 (46.24%), Query Frame = -3 Query: 900 TLDHLLAINVGSLGENMHFRRAV--ALKASKDSHLSIYSHLSWPDI-NKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIG--------------NSQADEPNESID---EENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGEL 1358 TL L A+ +G+LGEN+ RRA+ ++ H+ +Y H + GKYG ++ ++R+ + + NA + + QH+VG+ P+ +G + DE S D +E ++ Q FL D + V ++ + +E+ DF RF CGE+ Sbjct: 163 TLGDLTALTIGTLGENIQIRRAMYYSVPPIPTKHVGVYVHAPVAGTTGGQSGSCALGKYGALVAFRRK-------NTEFQNFNAAELGRRLGQHVVGMSPLTVGEMPEVREEEGEKKDGDKQDEEERSTDSDEDETQMLRQTFLLDPTMTVGEMTRQQGIELLDFVRFECGEV 328
BLAST of SMED30009566 vs. UniProt/SwissProt
Match: sp|A1L2P7|EFTS_XENLA (Elongation factor Ts, mitochondrial OS=Xenopus laevis OX=8355 GN=tsfm PE=2 SV=1) HSP 1 Score: 82.0333 bits (201), Expect = 8.406e-32 Identity = 43/123 (34.96%), Postives = 65/123 (52.85%), Query Frame = -2 Query: 1435 FYYXXXXXXXSKTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPD 1803 F+ + K L KLRK +G+ CK+AL +C ++ ++ WL +A K GW K KL + GL+G+ Q+ S VM+E+NCETDFVARN F + + + + H P+ Sbjct: 21 FHTGVRLLAADKDLLVKLRKKTGYSFMNCKKALEQCANDFKQAETWLHQQAQKEGWDKASKLQGRKTKEGLVGLL----QDGSTSVMVEVNCETDFVARNSKFQQLVQQVAVSTLRHCQSHPE 139 HSP 2 Score: 78.5666 bits (192), Expect = 8.406e-32 Identity = 51/162 (31.48%), Postives = 79/162 (48.77%), Query Frame = -3 Query: 897 MLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGELA 1382 +L L E+L LA+ +G LGENM +RA +K D + Y H TFGKYG ++ K D D L + N ++ + QH+VG++P+ +G+ + + E+ E ++ Q FL + +L V L + + DF RF CGE A Sbjct: 154 LLQLKADESLKDQLALAIGKLGENMIMKRAAWVKTPSDIFVGSYMHGILMADLPSLTNMTFGKYGALVICK---DSDGNL-----KSNISEIGRRLGQHVVGMNPLLVGSLEDESGGET---ETKMLAQPFLLEPSLTVGQYLQPRGINVLDFIRFECGEEA 304
BLAST of SMED30009566 vs. UniProt/SwissProt
Match: sp|B5X5B4|EFTS_SALSA (Elongation factor Ts, mitochondrial OS=Salmo salar OX=8030 GN=tsfm PE=2 SV=1) HSP 1 Score: 84.3445 bits (207), Expect = 5.427e-31 Identity = 43/106 (40.57%), Postives = 61/106 (57.55%), Query Frame = -2 Query: 1453 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEH 1770 K L KLRK +G+ CK+AL KC++++ ++ WL +A K GW K KL + GLIG+ + VM+E+NCETDFVARN+ F + + A M H Sbjct: 37 KALLMKLRKSTGYTFINCKKALEKCDNDITKAESWLHEQAQKEGWSKASKLEGRRAKEGLIGLFVGDK----AAVMVEVNCETDFVARNEKFQQLVKDVAFATMAH 138 HSP 2 Score: 73.559 bits (179), Expect = 5.427e-31 Identity = 54/165 (32.73%), Postives = 75/165 (45.45%), Query Frame = -3 Query: 894 ISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGELAT 1388 +S L L + +L LA+ +G LGEN+ RRAV + H+ Y H + G+YG ++ + Q D LD RK + QH+VG PV +GN D P D E L+ Q FL D + V+ L E + DF RF CGE + Sbjct: 159 LSKLNLGEDASLADQLALTIGRLGENISVRRAVTVGVPAGWHIGSYIHGGVAG----QSDMAMGRYGALVVF--QGGKDGALDT-LGRK--------LGQHVVGEAPVSLGNMD-DLP--CGDAETRLLPQSFLPDPSRTVAQYLTEQGARVLDFVRFQCGEAGS 305
BLAST of SMED30009566 vs. UniProt/SwissProt
Match: sp|A1A5Z3|EFTS_DANRE (Elongation factor Ts, mitochondrial OS=Danio rerio OX=7955 GN=tsfm PE=2 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 4.560e-30 Identity = 43/106 (40.57%), Postives = 63/106 (59.43%), Query Frame = -2 Query: 1453 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEH 1770 K L +LRK +G+ CK+AL KCN+++ ++ WL +A K GW K KL + GLIG+ ++ N VM+E+NCETDFVARN+ F + + + M H Sbjct: 37 KALLLQLRKSTGYTFVNCKKALEKCNNDITQAESWLHEQAKKEGWSKATKLEGRKAKEGLIGLMMHDN----AAVMVEVNCETDFVARNEKFQQLVKDVALSVMAH 138 HSP 2 Score: 66.2402 bits (160), Expect = 4.560e-30 Identity = 49/168 (29.17%), Postives = 71/168 (42.26%), Query Frame = -3 Query: 903 LNEYSISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGV-MLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1403 L+ +S L +L LA+ +G LGEN+ RRAV+L D H+ Y H T G+ G+ M +Y + + E + + QH++G PV +GN + D E L+ Q FL D V+ L + DF RF CGE Sbjct: 152 LSSEDMSKLNAPDGPSLADQLALTIGRLGENIAMRRAVSLSVPSDWHIGSYIH-----------GTVAGQVGIEMGRYGSLVVFQGEPKEGTY-----ALGRKLAQHVMGEAPVSLGNM---DDLSCGDSETRLLPQTFLPDPKYTVAQYLTLQDARVLDFIRFQCGE 300
BLAST of SMED30009566 vs. TrEMBL
Match: A0A183AQ99 (Elongation factor Ts, mitochondrial OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS9134 PE=3 SV=1) HSP 1 Score: 108.612 bits (270), Expect = 6.391e-46 Identity = 53/114 (46.49%), Postives = 74/114 (64.91%), Query Frame = -2 Query: 1429 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPDIN 1770 K+ LSKLR+ +G+ S CKEAL+K N+ D++ +WL EA + GW K GKL+ +P+S GL+G+ Q + V+LE+NCETDFVARN+ F + AT A ME P I+ Sbjct: 36 KSQLSKLRRSTGYTFSACKEALLKHNNEYDKALQWLNDEAVRRGWEKAGKLSTRPLSQGLLGVL----QTRTHAVVLEVNCETDFVARNEQFQNFVANATEAVMERFTVNPSIS 145 HSP 2 Score: 107.071 bits (266), Expect = 6.391e-46 Identity = 60/177 (33.90%), Postives = 100/177 (56.50%), Query Frame = -3 Query: 903 NSAQKNLNEYSISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKD-SHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIG---NSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1421 N + +L+ +I+ LIL K L +AI VG++GEN+ RRA+ L D S L+ Y+H+S + ++ FGKY +++Y+ + Q+ W + A++ +CQHIVG++P + G S AD+P DEE L+ Q F D+ ++V + L+ N + +E+F R CG+ Sbjct: 141 NPSISHLDAENINNLILEKSTKLSDAVAIVVGAVGENIAVRRAIGLHNPGDPSRLASYAHISTDGVKSGIRDVKFGKYAAIIRYR---PVGDQVASAAWHERASRLGKQLCQHIVGMNP-RPGLDLTSPADDP----DEEKCLLLQPFFLDDTVRVGEHLNRNEIVLEEFIRAECGQ 309
BLAST of SMED30009566 vs. TrEMBL
Match: A0A074Z3T0 (Elongation factor Ts, mitochondrial OS=Opisthorchis viverrini OX=6198 GN=T265_11234 PE=3 SV=1) HSP 1 Score: 108.227 bits (269), Expect = 6.826e-42 Identity = 56/166 (33.73%), Postives = 89/166 (53.61%), Query Frame = -3 Query: 903 ISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASK----DSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1388 +++ I T+ +A+ +G++GENM RRAV L+ S S L+ Y H+S I ++ FGKY +++Y+ Q W++ A++ +CQHIVG++P + EP E +EE L+ Q F DEN++V + L N M +EDF R CG+ Sbjct: 414 MALTIPNSAGTVSDNMAVAIGAIGENMAIRRAVGLRTSDIPDCASRLASYIHISTAGIRPSIRDVKFGKYAAVVRYR---PAGNQPASPAWQERASRLGEQLCQHIVGMNPRP--GLEITEPAEDPEEEQCLLLQPFFLDENIRVGEHLTRNDMILEDFIRVECGQ 574 HSP 2 Score: 93.9745 bits (232), Expect = 6.826e-42 Identity = 44/105 (41.90%), Postives = 69/105 (65.71%), Query Frame = -2 Query: 1456 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFME 1770 KT L+KLR+ +G+ + CKEAL+K +++++++ WL EA + GW K GKL+ + +S GL+G+Q + V++E+NCETDFVARN+ F + AT M Sbjct: 297 KTLLAKLRRQTGYTFAACKEALLKHDNDLNKASAWLTEEAVRRGWDKAGKLSGRTLSQGLLGLQFSRTH----AVVVEVNCETDFVARNEQFQNFVAVATEMVMR 397 HSP 3 Score: 95.1301 bits (235), Expect = 1.371e-16 Identity = 47/116 (40.52%), Postives = 72/116 (62.07%), Query Frame = -2 Query: 1432 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICEL---PDI 1770 KT L+KLR+ +G+ + CKEAL+K +++++++ WL EA + GW K GKL+ + +S GL+G+Q + V++E+NCETDFVARN+ F + AT M PDI Sbjct: 109 KTLLAKLRRQTGYTFAACKEALLKHDNDLNKASAWLTEEAVRRGWDKAGKLSGRTLSQGLLGLQFSRTH----AVVVEVNCETDFVARNEQFQNFVAVATEMVMREFVSRFISPDI 220
BLAST of SMED30009566 vs. TrEMBL
Match: A0A504YKM2 (Elongation factor Ts, mitochondrial OS=Fasciola gigantica OX=46835 GN=FGIG_01136 PE=3 SV=1) HSP 1 Score: 103.605 bits (257), Expect = 7.117e-42 Identity = 56/163 (34.36%), Postives = 91/163 (55.83%), Query Frame = -3 Query: 903 LILTKKETLDHLLAINVGSLGENMHFRRAVALKASKD-SHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIG---NSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1379 ++L K L +AI VG++GEN+ RRAV L D S L+ Y+H+S + + +E FGKY +++Y+ Q+ W + A++ +CQHIVG++P + G + AD+P DEE L+ Q F D+ ++V + L N + +E+F R CG+ Sbjct: 154 MVLDKSTKLSDAVAIVVGAVGENIAVRRAVGLHNIDDTSRLASYAHISTDGVKQGIREVRFGKYAAVIRYR---PTGEQIASAAWHERASRLGKQLCQHIVGMNP-RPGLELTTPADDP----DEEKCLLLQPFFLDDTVRVGEHLARNEIVLEEFVRLECGQ 308 HSP 2 Score: 98.5969 bits (244), Expect = 7.117e-42 Identity = 48/114 (42.11%), Postives = 71/114 (62.28%), Query Frame = -2 Query: 1429 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPDIN 1770 K+ LS+LR+ +G+ C+EAL+K N++ D + +WL EA + GW K KL+ + +S GL+G+ Q + V++E+NCETDFVARN+ F + AT A ME P IN Sbjct: 35 KSHLSQLRRSTGYTFGACREALLKHNNDFDRALQWLNEEAIRRGWDKAEKLSTRHLSQGLLGVL----QTRTHAVVIEVNCETDFVARNEQFQNLVAGATEAIMERFVINPSIN 144
BLAST of SMED30009566 vs. TrEMBL
Match: A0A4S2LRI2 (Elongation factor Ts, mitochondrial OS=Opisthorchis felineus OX=147828 GN=CRM22_006811 PE=3 SV=1) HSP 1 Score: 107.071 bits (266), Expect = 7.292e-42 Identity = 56/166 (33.73%), Postives = 89/166 (53.61%), Query Frame = -3 Query: 903 ISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASK----DSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1388 +++ I T+ +A+ +G++GENM RRAV L+ S S L+ Y H+S I ++ FGKY +++Y+ Q W++ A++ +CQHIVG++P + EP E +EE L+ Q F DEN++V + L N M +EDF R CG+ Sbjct: 147 MALTIPNSGGTVSDNIAVAIGAVGENMAIRRAVGLRTSDIPDCTSRLASYIHISTAGIRPGIRDVKFGKYAAVVRYR---PAGNQPASPAWQERASRLGEQLCQHIVGMNPRP--GLEITEPAEDPEEEQCLLLQPFFLDENIRVGEHLTRNDMILEDFIRVECGQ 307 HSP 2 Score: 95.1301 bits (235), Expect = 7.292e-42 Identity = 45/109 (41.28%), Postives = 70/109 (64.22%), Query Frame = -2 Query: 1444 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICE 1770 KT L+KLR+ +G+ + CKEAL+K +++++++ WL EA + GW K GKL+ + +S GL+G+Q + V++E+NCETDFVARN+ F + AT M E Sbjct: 30 KTLLAKLRRQTGYTFAACKEALLKHDNDLNKASAWLTEEAVRRGWDKAGKLSGRTLSQGLLGLQFSRTH----AVVVEVNCETDFVARNEQFQNFVAMATEMVMREFAE 134
BLAST of SMED30009566 vs. TrEMBL
Match: A0A0R3SDW0 (Elongation factor Ts, mitochondrial OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS2902 PE=3 SV=1) HSP 1 Score: 107.842 bits (268), Expect = 8.465e-42 Identity = 58/167 (34.73%), Postives = 93/167 (55.69%), Query Frame = -3 Query: 906 SISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDS-----HLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCG 1391 +I ++ ++++T+ LLA +GSLGENM R V + S D +L+ Y H+S I K ++ FGKY +L+Y+ + Q W + A++ +CQHIVG++P + + A E+ DEE L+ Q FL DE++ V +L +N + +EDF R CG Sbjct: 150 AIPNVVGSQQQTIGELLANIIGSLGENMAIPRGVGMALSGDKANEFRYLTAYCHMSNTGIKKGVRDVVFGKYIAILRYRH---MSNQKGSAAWHEQASRFTQQLCQHIVGMNPHRDLSLDAIPQAENPDEEKCLLRQGFLFDESVPVGTLLEQNEIVVEDFVRVECG 313 HSP 2 Score: 93.9745 bits (232), Expect = 8.465e-42 Identity = 48/123 (39.02%), Postives = 70/123 (56.91%), Query Frame = -2 Query: 1393 LSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPDINAIQLGSEELKRI 1761 L++LRK +G P C+EAL CN + D + WL++EA K G K KL +PMS GL+GM + C ++E+NCETDFVARNQ+F + ++T + + E ++LK I Sbjct: 33 LAQLRKATGHPFVFCREALAVCNGDYDRALAWLQNEATKRGMTKAEKLKLRPMSQGLLGMIASTR----CVAVVEVNCETDFVARNQDFQYLVASSTESLFKFFSEANQPGTHMFSGDDLKAI 151
BLAST of SMED30009566 vs. Ensembl Cavefish
Match: tsfm (Ts translation elongation factor, mitochondrial [Source:NCBI gene;Acc:103022184]) HSP 1 Score: 87.4261 bits (215), Expect = 1.108e-29 Identity = 46/108 (42.59%), Postives = 63/108 (58.33%), Query Frame = -2 Query: 1447 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHIC 1770 K L KLRK +G+ CK+AL K N+++ ++ WL +A K GW K KL + GLIG+ +N S VM+E+NCETDFVARN+ F + + A M H C Sbjct: 32 KALLLKLRKATGYTFINCKKALEKFNNDIAQAESWLHEQAQKEGWSKASKLEGRRAKEGLIGVL----KNDSAAVMVEVNCETDFVARNEKFQQLVQDVALATMAHHC 135 HSP 2 Score: 63.1586 bits (152), Expect = 1.108e-29 Identity = 45/169 (26.63%), Postives = 75/169 (44.38%), Query Frame = -3 Query: 903 LNEYSISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSH--LSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1403 L+ ++ L + + +L LA+ +G LGEN+ RRAV++ D H+ Y H ++ P G+YG ++ ++ + + + + QHIVG P +GN + ES E L+ Q FL + V L + + DF R+ CGE Sbjct: 147 LSAEDLAKLTMVEGPSLADQLALTIGRLGENISIRRAVSIGIPSDWHIGSYVHSPVTGPS------SLMMGRYGSLVVFQGGPEGEVEF-----------LGKKLGQHIVGEAPDTLGNMEDLPCGES---ETRLLPQTFLLEPKYTVGQFLTLHNARVLDFIRYKCGE 295
BLAST of SMED30009566 vs. Ensembl Nematostella
Match: EDO34261 (Elongation factor Ts, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:A7SPW6]) HSP 1 Score: 96.6709 bits (239), Expect = 4.130e-22 Identity = 48/106 (45.28%), Postives = 68/106 (64.15%), Query Frame = -2 Query: 1453 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEH 1770 K+ L KLRK +GF SKC+EALV ++ + WL +A K GW K KL + + GLIG+ +N + + + G M+E+NCETDFVARN+NF +NT TS + + Sbjct: 3 KSLLGKLRKETGFGFSKCREALVLARNDYAAAEAWLHEQAEKEGWQKANKLQGRSATEGLIGVIVN-HSDMNLGAMVEVNCETDFVARNENFVDLVNTVTSTTLAY 107 HSP 2 Score: 74.3294 bits (181), Expect = 1.385e-14 Identity = 46/151 (30.46%), Postives = 74/151 (49.01%), Query Frame = -3 Query: 906 LDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKR-QIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCG 1355 L ++A +G LGEN+ +A+ + D+ + Y H + K + +FGKYG M+ K + IDT + AN + QH+VG++P IG Q E +E E L+ Q +L D +L V + +++ DF R+ CG Sbjct: 152 LSDMVAKVIGKLGENIKLGKAITITTDSDNVIGSYVHGPYV---TKVHQCSFGKYGAMVAVKPIKKGIDTS--------SLALLANKLAQHVVGMNPKVIG--QGGEADEKGGESEALLDQEYLLDGSLTVGQFTEKEGVQVVDFVRYECG 289
BLAST of SMED30009566 vs. Ensembl Medaka
Match: tsfm (Ts translation elongation factor, mitochondrial [Source:NCBI gene;Acc:101160042]) HSP 1 Score: 77.411 bits (189), Expect = 1.438e-27 Identity = 41/106 (38.68%), Postives = 60/106 (56.60%), Query Frame = -2 Query: 1453 KTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEH 1770 K L KLRK +G+ CK+AL +++ ++ WL +A K GW K KL + GLIG+ + + VM+E+NCETDFVARN F + + T A ++H Sbjct: 38 KALLMKLRKSTGYTFINCKKALEMFDNDPAQAESWLHEQAQKEGWSKANKLEGRKAKEGLIGVFVGD----AAAVMVEVNCETDFVARNDKFQQLVKDVTFATLDH 139 HSP 2 Score: 65.855 bits (159), Expect = 1.438e-27 Identity = 46/162 (28.40%), Postives = 77/162 (47.53%), Query Frame = -3 Query: 903 ISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGE 1388 +S L + + L +A+ +G LGENM +RAV + + + Y H ++ + +E G+YG ++ ++ + + + RK + QHIVG P+ +GN ES E L+ Q FL D + V++ L ++ DF RF CGE Sbjct: 161 LSRLRMGEGAALADSVALTIGRLGENMSVKRAVMVGVPAEWRIGSYVH---GNVGSQ-QEVAMGRYGALVIFQGGKEGEQDM---LGRK--------LGQHIVGEAPLSLGNMDDLPCGES---ETRLLPQTFLADPSRTVAEFLRGQQAKVLDFVRFQCGE 304
BLAST of SMED30009566 vs. Planmine SMEST
Match: SMESG000015985.1 (SMESG000015985.1) HSP 1 Score: 339.347 bits (869), Expect = 3.997e-177 Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = -3 Query: 888 KKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGELATFA 1367 KKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGELATFA Sbjct: 164 KKETLDHLLAINVGSLGENMHFRRAVALKASKDSHLSIYSHLSWPDINKKWKETTFGKYGVMLKYKRQIDIDTQLDEDCWRKNATKAANAICQHIVGLDPVKIGNSQADEPNESIDEENILIYQRFLHDENLKVSDVLHENCMEIEDFTRFNCGELATFA 323 HSP 2 Score: 302.753 bits (774), Expect = 3.997e-177 Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = -2 Query: 1363 TYKLIGNKFYYXXXXXXXSKTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPDINAIQLGSEELKRILYLDVDSNKK 1827 TYKLIGNKFYYSSSSHSISKTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPDINAIQLGSEELKRILYLDVDSNKK Sbjct: 11 TYKLIGNKFYYSSSSHSISKTDLSKLRKISGFPISKCKEALVKCNSNVDESHEWLKSEAAKHGWLKMGKLAEKPMSNGLIGMQLNANQNFSCGVMLELNCETDFVARNQNFHKCLNTATSAFMEHICELPDINAIQLGSEELKRILYLDVDSNKK 165 The following BLAST results are available for this feature:
BLAST of SMED30009566 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30009566 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of SMED30009566 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of SMED30009566 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of SMED30009566 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of SMED30009566 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 3
BLAST of SMED30009566 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30009566 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30009566 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of SMED30009566 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30009566 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30009566 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30009566 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of SMED30009566 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009566 ID=SMED30009566|Name=SMED30009566|organism=Schmidtea mediterranea sexual|type=transcript|length=1990bpback to top protein sequence of SMED30009566-orf-1 >SMED30009566-orf-1 ID=SMED30009566-orf-1|Name=SMED30009566-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=182bp MQFNSAQKNLNEYSISMLILTKKETLDHLLAINVGSLGENMHFRRAVALKback to top protein sequence of SMED30009566-orf-2 >SMED30009566-orf-2 ID=SMED30009566-orf-2|Name=SMED30009566-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=175bp MFNLNLRNLNTYKLIGNKFYYSSSSHSISKTDLSKLRKISGFPISKCKEAback to top protein sequence of SMED30009566-orf-3 >SMED30009566-orf-3 ID=SMED30009566-orf-3|Name=SMED30009566-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=291bp MIGNQYEISNLKGYLLSKEHRYNHELVRPSGRHLDSGYTNQKVHHQVNKIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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