Peptidase_M43 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009546 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of Peptidase_M43 domain-containing protein vs. TrEMBL
Match: A0A433QY88 (Uncharacterized protein OS=Jimgerdemannia flammicorona OX=994334 GN=BC938DRAFT_478572 PE=4 SV=1) HSP 1 Score: 59.3066 bits (142), Expect = 8.764e-8 Identity = 24/47 (51.06%), Postives = 29/47 (61.70%), Query Frame = 1 Query: 727 NRRTENVWYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALCQR 867 N W GTAP C+GSCP+G T I GDG CWTG+K LC++ Sbjct: 23 NGECSRYWSGTAPFCAGSCPEGYTEITRSSCGDGACCWTGYKVLCEK 69
BLAST of Peptidase_M43 domain-containing protein vs. TrEMBL
Match: A0A2A6BVN9 (Uncharacterized protein OS=Pristionchus pacificus OX=54126 GN=PRIPAC_49186 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 4.490e-7 Identity = 24/46 (52.17%), Postives = 27/46 (58.70%), Query Frame = 1 Query: 736 TENVWYGTAPAC-SGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRV 870 T WYGTAP C CP G T ++ D GDG TCW G K LC+ V Sbjct: 25 TTYAWYGTAPFCFPEDCPDGWTFVKNDDKGDGSTCWIGEKTLCKFV 70
BLAST of Peptidase_M43 domain-containing protein vs. TrEMBL
Match: A0A4X3PKU5 (Uncharacterized protein OS=Pristionchus pacificus OX=54126 GN=WBGene00281063 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 4.490e-7 Identity = 24/46 (52.17%), Postives = 27/46 (58.70%), Query Frame = 1 Query: 736 TENVWYGTAPAC-SGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRV 870 T WYGTAP C CP G T ++ D GDG TCW G K LC+ V Sbjct: 25 TTYAWYGTAPFCFPEDCPDGWTFVKNDDKGDGSTCWIGEKTLCKFV 70
BLAST of Peptidase_M43 domain-containing protein vs. TrEMBL
Match: S7RWA5 (Uncharacterized protein OS=Gloeophyllum trabeum (strain ATCC 11539 / FP-39264 / Madison 617) OX=670483 GN=GLOTRDRAFT_127937 PE=4 SV=1) HSP 1 Score: 55.0694 bits (131), Expect = 3.883e-6 Identity = 21/44 (47.73%), Postives = 28/44 (63.64%), Query Frame = 1 Query: 748 WYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRVYFI 879 W GT+P C+GSCP+G T T GDG CWTG+K + R+ + Sbjct: 44 WSGTSPFCAGSCPEGYTDTATSSCGDGACCWTGYKNVVMRIKLV 87
BLAST of Peptidase_M43 domain-containing protein vs. TrEMBL
Match: A0A1C7MHJ3 (Uncharacterized protein OS=Grifola frondosa OX=5627 GN=A0H81_02939 PE=4 SV=1) HSP 1 Score: 56.225 bits (134), Expect = 9.871e-6 Identity = 22/38 (57.89%), Postives = 28/38 (73.68%), Query Frame = 1 Query: 748 WYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALC 861 WYGTAP C+G CP G+T + TD SGDG +C +G+K C Sbjct: 119 WYGTAPICAGECPPGKTQVTTDGSGDGASCVSGNKVFC 156
BLAST of Peptidase_M43 domain-containing protein vs. Planmine SMEST
Match: SMESG000019773.1 (SMESG000019773.1) HSP 1 Score: 452.595 bits (1163), Expect = 1.627e-162 Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 1 Query: 199 MATSHSSITRSYLAMKXXXXXXXXXXAFTVYAERPKQCVFPFTYKGRTFEDCTDVNADFLWCSPTKEYSGTSIPCNSEEEQQRLKRDNYYKLFKILEEQEEHIKKLEASHSTNTKCSNCKEEADAIVKSLGALSSIASDHLRKITALKDKFEKKQKFADALLTKVQQINELALRANNRRTENVWYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRVYFI 879 MATSHSSITRSYLAMKLHLIILLLCIAFTVYAERPKQCVFPFTYKGRTFEDCTDVNADFLWCSPTKEYSGTSIPCNSEEEQQRLKRDNYYKLFKILEEQEEHIKKLEASHSTNTKCSNCKEEADAIVKSLGALSSIASDHLRKITALKDKFEKKQKFADALLTKVQQINELALRANNRRTENVWYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRVYFI Sbjct: 1 MATSHSSITRSYLAMKLHLIILLLCIAFTVYAERPKQCVFPFTYKGRTFEDCTDVNADFLWCSPTKEYSGTSIPCNSEEEQQRLKRDNYYKLFKILEEQEEHIKKLEASHSTNTKCSNCKEEADAIVKSLGALSSIASDHLRKITALKDKFEKKQKFADALLTKVQQINELALRANNRRTENVWYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRVYFI 227
BLAST of Peptidase_M43 domain-containing protein vs. Planmine SMEST
Match: SMESG000019773.1 (SMESG000019773.1) HSP 1 Score: 422.165 bits (1084), Expect = 1.393e-150 Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 1 Query: 277 AFTVYAERPKQCVFPFTYKGRTFEDCTDVNADFLWCSPTKEYSGTSIPCNSEEEQQRLKRDNYYKLFKILEEQEEHIKKLEASHSTNTKCSNCKEEADAIVKSLGALSSIASDHLRKITALKDKFEKKQKFADALLTKVQQINELALRANNRRTENVWYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRVYFI 879 AFTVYAERPKQCVFPFTYKGRTFEDCTDVNADFLWCSPTKEYSGTSIPCNSEEEQQRLKRDNYYKLFKILEEQEEHIKKLEASHSTNTKCSNCKEEADAIVKSLGALSSIASDHLRKITALKDKFEKKQKFADALLTKVQQINELALRANNRRTENVWYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRVYFI Sbjct: 13 AFTVYAERPKQCVFPFTYKGRTFEDCTDVNADFLWCSPTKEYSGTSIPCNSEEEQQRLKRDNYYKLFKILEEQEEHIKKLEASHSTNTKCSNCKEEADAIVKSLGALSSIASDHLRKITALKDKFEKKQKFADALLTKVQQINELALRANNRRTENVWYGTAPACSGSCPQGQTLIRTDKSGDGHTCWTGHKALCQRVYFI 213
BLAST of Peptidase_M43 domain-containing protein vs. Planmine SMEST
Match: SMESG000034284.1 (SMESG000034284.1) HSP 1 Score: 70.8626 bits (172), Expect = 1.846e-13 Identity = 46/191 (24.08%), Postives = 89/191 (46.60%), Query Frame = 1 Query: 292 AERPKQCVFPFTYKGRTFEDCTDVNADFLWCSPTKEYSGTSIPCNSEEEQQRLKRDNYYKLFKILEEQEEHIKKLEAS-HSTNTKCSNCKEEADAIVKSLGALSSIASDHLRKITALKDKFEKKQKFADAL------------LTKVQQINELALRANNRRTENVWYGTAPACSGSCPQGQTLIRTDKSGD 825 +P C FPF YKG T+ +C ++ + WCS + Y+GT C E++ ++ ++Y+L K +E + KL+ + ++ N +CS C E I ++ S+I D +++ L+ + K +K D + T ++ I +++ A+ + +N W S P + +D+ D Sbjct: 20 VNKPATCTFPFQYKGATYNECIKFDSQYYWCSVYEIYNGTFAYC-QEDKTDQITDQHFYELMKKSQEAVSRLDKLQQNLNNKNGQCSQCIEIMKTISTAITDQSAIYMDQNKRLLGLRAQHSKLEKVVDQIKQHYQKRRCKQSPTGIEYIGRVSMTASGFQCQN-W-------SSQFPNKHEYVESDRYSD 201
BLAST of Peptidase_M43 domain-containing protein vs. Planmine SMEST
Match: SMESG000028600.1 (SMESG000028600.1) HSP 1 Score: 63.5438 bits (153), Expect = 3.373e-11 Identity = 44/138 (31.88%), Postives = 61/138 (44.20%), Query Frame = 1 Query: 289 YAERPKQCVFPFTYKGRTFEDCTDVNADFLWCSPTKEYSGTSIPCNSEEEQQRLKRDNYYKLFKILEEQEEHIKKLEASHSTNTKCSNCKEEADAIVKSLGALSSIASDHLRKITALKDKFEKKQKFADALLTKVQQI 702 Y + PK CVFPF Y+GR ++DC +A WCS EY G CN+ E + + K L E E +K S + C C + S+ LS I+ D+ R I L + + + F DAL I Sbjct: 21 YKQHPK-CVFPFKYQGRVYDDCVVKDAGAPWCSEDAEYKGLFHYCNNNEGDTQESNGAFLSAQKKLAENSEILKN--KSFISGDSCETCGTQFYITESSMTQLSKISDDNNRHIDYLLNV--ENENFKDALGISFNDI 153 The following BLAST results are available for this feature:
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Peptidase_M43 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009546 ID=SMED30009546|Name=Peptidase_M43 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=990bpback to top protein sequence of SMED30009546-orf-1 >SMED30009546-orf-1 ID=SMED30009546-orf-1|Name=SMED30009546-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=124bp FFLRQHFVFNNNRIRLKTQVKRIQGVIYMSTQRFACLYKINTLAKGLMSSback to top protein sequence of SMED30009546-orf-2 >SMED30009546-orf-2 ID=SMED30009546-orf-2|Name=SMED30009546-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=228bp MATSHSSITRSYLAMKLHLIILLLCIAFTVYAERPKQCVFPFTYKGRTFEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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