Complexin-1/2

Overview
NameComplexin-1/2
Smed IDSMED30009438
Length (bp)893
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Complexin-1/2 (SMED30009438) t-SNE clustered cells

Violin plots show distribution of expression levels for Complexin-1/2 (SMED30009438) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Complexin-1/2 (SMED30009438) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Complexin-1/2 (SMED30009438) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009438

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30009438SMESG000012002.1 SmedASXL_008790SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30009438SMESG000012002.1 dd_Smed_v4_9918_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30009438SMESG000012002.1 dd_Smed_v4_9918_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30009438SMESG000012002.1 dd_Smed_v4_9918_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30009438SMESG000012002.1 dd_Smed_v4_9918_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30009438SMESG000012002.1 dd_Smed_v6_9918_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30009438SMESG000012002.1 OX_Smed_1.0.02700ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Complexin-1/2 vs. Ensembl Human
Match: CPLX1 (complexin 1 [Source:HGNC Symbol;Acc:HGNC:2309])

HSP 1 Score: 50.447 bits (119), Expect = 1.541e-7
Identity = 34/81 (41.98%), Postives = 46/81 (56.79%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKK--ENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSC--EDDTIIGNV 605
            A+R+ EE+R  K+ KME ERE +RQGIRDKYG+KKK             + EG   R +K+     GD    ED++I+  V
Sbjct:   25 ALRQAEEERKAKYAKMEAEREAVRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTV 105
[Close]
BLAST of Complexin-1/2 vs. Ensembl Human
Match: CPLX1 (complexin 1 [Source:HGNC Symbol;Acc:HGNC:2309])

HSP 1 Score: 50.8322 bits (120), Expect = 1.878e-7
Identity = 34/81 (41.98%), Postives = 46/81 (56.79%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKK--ENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSC--EDDTIIGNV 605
            A+R+ EE+R  K+ KME ERE +RQGIRDKYG+KKK             + EG   R +K+     GD    ED++I+  V
Sbjct:   40 ALRQAEEERKAKYAKMEAEREAVRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTV 120
[Close]
BLAST of Complexin-1/2 vs. Ensembl Human
Match: CPLX2 (complexin 2 [Source:HGNC Symbol;Acc:HGNC:2310])

HSP 1 Score: 49.2914 bits (116), Expect = 2.100e-7
Identity = 26/41 (63.41%), Postives = 32/41 (78.05%), Query Frame = 3 
Query:  363 EIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKE 485
            E + A+R+ EE+R  KH +ME ERE +RQ IRDKYGLKKKE
Sbjct:   36 ERQEALRQQEEERKAKHARMEAEREKVRQQIRDKYGLKKKE 76
[Close]
BLAST of Complexin-1/2 vs. Ensembl Celegans
Match: cpx-1 (Putative complexin-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9GUM7])

HSP 1 Score: 86.6557 bits (213), Expect = 9.640e-21
Identity = 62/137 (45.26%), Postives = 80/137 (58.39%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGD-SCEDDTIIGNVKGFANRVTEVPKQLVTEA 659
            MA F+ K +VG QL  V G LG K D    ++   DPE+ AA  E EE+R +KHRKME ERE MRQGIRDKY +KKKE    MD      EG+    RK+  +   + + EDD++IG + G   +V E  K + T A
Sbjct:    1 MAGFLMKQMVGNQLSEVTGGLGMKDDGGEKTETGEDPEVIAARLEQEERRKEKHRKMENEREKMRQGIRDKYAIKKKEEGVAMD----FTEGRIGGPRKTPEEIAAEMNAEDDSLIGQL-GLTEQV-EKAKTMATGA 131
[Close]
BLAST of Complexin-1/2 vs. Ensembl Fly
Match: cpx (gene:FBgn0041605 transcript:FBtr0336482)

HSP 1 Score: 69.707 bits (169), Expect = 2.408e-14
Identity = 45/81 (55.56%), Postives = 57/81 (70.37%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAA---IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKE 485
            MA FIAK +VG QL +VKGA+G  G ++ D K + + E       I+E E++R +KHRKMEEERE MRQ IRDKY +KKKE
Sbjct:    1 MAAFIAKQMVGNQLSAVKGAVGGDGGDDGDDKEKAEEEERERQEAIKEAEDRRKEKHRKMEEEREKMRQDIRDKYNIKKKE 81
[Close]
BLAST of Complexin-1/2 vs. Ensembl Fly
Match: cpx (gene:FBgn0041605 transcript:FBtr0078901)

HSP 1 Score: 69.3218 bits (168), Expect = 3.095e-14
Identity = 45/83 (54.22%), Postives = 57/83 (68.67%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAA---IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENF 491
            MA FIAK +VG QL +VKGA+G  G ++ D K + + E       I+E E++R +KHRKMEEERE MRQ IRDKY +KKKE  
Sbjct:    1 MAAFIAKQMVGNQLSAVKGAVGGDGGDDGDDKEKAEEEERERQEAIKEAEDRRKEKHRKMEEEREKMRQDIRDKYNIKKKEEI 83
[Close]
BLAST of Complexin-1/2 vs. Ensembl Fly
Match: cpx (gene:FBgn0041605 transcript:FBtr0301769)

HSP 1 Score: 69.3218 bits (168), Expect = 3.095e-14
Identity = 45/83 (54.22%), Postives = 57/83 (68.67%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAA---IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENF 491
            MA FIAK +VG QL +VKGA+G  G ++ D K + + E       I+E E++R +KHRKMEEERE MRQ IRDKY +KKKE  
Sbjct:    1 MAAFIAKQMVGNQLSAVKGAVGGDGGDDGDDKEKAEEEERERQEAIKEAEDRRKEKHRKMEEEREKMRQDIRDKYNIKKKEEI 83
[Close]
BLAST of Complexin-1/2 vs. Ensembl Fly
Match: cpx (gene:FBgn0041605 transcript:FBtr0301771)

HSP 1 Score: 69.3218 bits (168), Expect = 3.095e-14
Identity = 45/83 (54.22%), Postives = 57/83 (68.67%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAA---IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENF 491
            MA FIAK +VG QL +VKGA+G  G ++ D K + + E       I+E E++R +KHRKMEEERE MRQ IRDKY +KKKE  
Sbjct:    1 MAAFIAKQMVGNQLSAVKGAVGGDGGDDGDDKEKAEEEERERQEAIKEAEDRRKEKHRKMEEEREKMRQDIRDKYNIKKKEEI 83
[Close]
BLAST of Complexin-1/2 vs. Ensembl Fly
Match: cpx (gene:FBgn0041605 transcript:FBtr0078899)

HSP 1 Score: 69.3218 bits (168), Expect = 3.095e-14
Identity = 45/83 (54.22%), Postives = 57/83 (68.67%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAA---IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENF 491
            MA FIAK +VG QL +VKGA+G  G ++ D K + + E       I+E E++R +KHRKMEEERE MRQ IRDKY +KKKE  
Sbjct:    1 MAAFIAKQMVGNQLSAVKGAVGGDGGDDGDDKEKAEEEERERQEAIKEAEDRRKEKHRKMEEEREKMRQDIRDKYNIKKKEEI 83
[Close]
BLAST of Complexin-1/2 vs. Ensembl Zebrafish
Match: cplx2l (complexin 2, like [Source:ZFIN;Acc:ZDB-GENE-040718-160])

HSP 1 Score: 51.6026 bits (122), Expect = 7.416e-8
Identity = 32/70 (45.71%), Postives = 43/70 (61.43%), Query Frame = 3 
Query:  378 IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSCEDD 587
            +R+ EE+R  KH KME EREN+RQGIRDKYG+KK+E     +     +  + + TR     P+G  C DD
Sbjct:   42 LRQEEEERKAKHAKMEAERENIRQGIRDKYGIKKREVAEAEEAAAMEQACEGSMTRPKKAVPSG--CGDD 109
[Close]
BLAST of Complexin-1/2 vs. Ensembl Zebrafish
Match: cplx2l (complexin 2, like [Source:ZFIN;Acc:ZDB-GENE-040718-160])

HSP 1 Score: 51.6026 bits (122), Expect = 7.416e-8
Identity = 32/70 (45.71%), Postives = 43/70 (61.43%), Query Frame = 3 
Query:  378 IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSCEDD 587
            +R+ EE+R  KH KME EREN+RQGIRDKYG+KK+E     +     +  + + TR     P+G  C DD
Sbjct:   42 LRQEEEERKAKHAKMEAERENIRQGIRDKYGIKKREVAEAEEAAAMEQACEGSMTRPKKAVPSG--CGDD 109
[Close]
BLAST of Complexin-1/2 vs. Ensembl Zebrafish
Match: cplx2l (complexin 2, like [Source:ZFIN;Acc:ZDB-GENE-040718-160])

HSP 1 Score: 51.6026 bits (122), Expect = 7.416e-8
Identity = 32/70 (45.71%), Postives = 43/70 (61.43%), Query Frame = 3 
Query:  378 IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSCEDD 587
            +R+ EE+R  KH KME EREN+RQGIRDKYG+KK+E     +     +  + + TR     P+G  C DD
Sbjct:   42 LRQEEEERKAKHAKMEAERENIRQGIRDKYGIKKREVAEAEEAAAMEQACEGSMTRPKKAVPSG--CGDD 109
[Close]
BLAST of Complexin-1/2 vs. Ensembl Zebrafish
Match: cplx2l (complexin 2, like [Source:ZFIN;Acc:ZDB-GENE-040718-160])

HSP 1 Score: 51.6026 bits (122), Expect = 7.416e-8
Identity = 32/70 (45.71%), Postives = 43/70 (61.43%), Query Frame = 3 
Query:  378 IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSCEDD 587
            +R+ EE+R  KH KME EREN+RQGIRDKYG+KK+E     +     +  + + TR     P+G  C DD
Sbjct:   42 LRQEEEERKAKHAKMEAERENIRQGIRDKYGIKKREVAEAEEAAAMEQACEGSMTRPKKAVPSG--CGDD 109
[Close]
BLAST of Complexin-1/2 vs. Ensembl Zebrafish
Match: cplx2 (complexin 2 [Source:ZFIN;Acc:ZDB-GENE-081113-1])

HSP 1 Score: 45.8246 bits (107), Expect = 7.080e-6
Identity = 21/33 (63.64%), Postives = 28/33 (84.85%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGL 473
            A+R+ EE+R  K+ KME ERE++RQGIRDKYG+
Sbjct:   40 ALRQQEEERKAKYAKMEAERESVRQGIRDKYGI 72
[Close]
BLAST of Complexin-1/2 vs. Ensembl Xenopus
Match: cplx1 (complexin 1 [Source:NCBI gene;Acc:100124828])

HSP 1 Score: 46.2098 bits (108), Expect = 6.799e-6
Identity = 22/37 (59.46%), Postives = 29/37 (78.38%), Query Frame = 3 
Query:  363 EIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGL 473
            E + A+R+ E++R  K+ KME ERE MRQGIRDKYG+
Sbjct:   36 EAQEALRQQEDERKAKYAKMEAEREIMRQGIRDKYGI 72
[Close]
BLAST of Complexin-1/2 vs. Ensembl Mouse
Match: Cplx1 (complexin 1 [Source:MGI Symbol;Acc:MGI:104727])

HSP 1 Score: 51.2174 bits (121), Expect = 8.908e-8
Identity = 35/81 (43.21%), Postives = 46/81 (56.79%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKK--ENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSC--EDDTIIGNV 605
            A+R+ EE+R  K+ KME ERE MRQGIRDKYG+KKK             + EG   R +K+     GD    ED++I+  V
Sbjct:   40 ALRQAEEERKAKYAKMEAEREVMRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEPEEEDESILDTV 120
[Close]
BLAST of Complexin-1/2 vs. Ensembl Mouse
Match: Cplx1 (complexin 1 [Source:MGI Symbol;Acc:MGI:104727])

HSP 1 Score: 48.9062 bits (115), Expect = 2.047e-7
Identity = 26/40 (65.00%), Postives = 32/40 (80.00%), Query Frame = 3 
Query:  363 EIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKK 482
            E + A+R+ EE+R  K+ KME ERE MRQGIRDKYG+KKK
Sbjct:   21 ERQEALRQAEEERKAKYAKMEAEREVMRQGIRDKYGIKKK 60
[Close]
BLAST of Complexin-1/2 vs. UniProt/SwissProt
Match: sp|Q9GUM7|CPLX1_CAEEL (Putative complexin-1 OS=Caenorhabditis elegans OX=6239 GN=cpx-1 PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.226e-19
Identity = 62/137 (45.26%), Postives = 80/137 (58.39%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGD-SCEDDTIIGNVKGFANRVTEVPKQLVTEA 659
            MA F+ K +VG QL  V G LG K D    ++   DPE+ AA  E EE+R +KHRKME ERE MRQGIRDKY +KKKE    MD      EG+    RK+  +   + + EDD++IG + G   +V E  K + T A
Sbjct:    1 MAGFLMKQMVGNQLSEVTGGLGMKDDGGEKTETGEDPEVIAARLEQEERRKEKHRKMENEREKMRQGIRDKYAIKKKEEGVAMD----FTEGRIGGPRKTPEEIAAEMNAEDDSLIGQL-GLTEQV-EKAKTMATGA 131
[Close]
BLAST of Complexin-1/2 vs. UniProt/SwissProt
Match: sp|Q60PP8|CPLX1_CAEBR (Putative complexin-1 OS=Caenorhabditis briggsae OX=6238 GN=cpx-1 PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.339e-18
Identity = 63/137 (45.99%), Postives = 82/137 (59.85%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGD-SCEDDTIIGNVKGFANRVTEVPKQLVTEA 659
            MA F+ K +VG QL+ V G LG K D    ++   DPE+ AA  E EE+R +KHRKME+ERE MRQGIRDKY +KKKE    MD      EG+    RK+  +   + + EDD+IIG + G   +V E  K + T A
Sbjct:    1 MAGFLMKQMVGNQLNEVTGGLGLKDDGGEKTETGEDPEVVAARLEQEERRKEKHRKMEQEREKMRQGIRDKYAIKKKEEGVAMD----FTEGRIGGPRKTPEEIAAEMNAEDDSIIGQL-GLTEQV-EKAKTMATGA 131
[Close]
BLAST of Complexin-1/2 vs. UniProt/SwissProt
Match: sp|Q8I6U3|CPLX_HIRME (Complexin-1 OS=Hirudo medicinalis OX=6421 GN=cpx1 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.762e-16
Identity = 53/115 (46.09%), Postives = 66/115 (57.39%), Query Frame = 3 
Query:  360 PEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKST------LDPNGDSCEDDTIIGNVKGFANRVTEVPKQLVTE---ATDKCHL 677
            PEI  A RE EEKR +K+RKMEEERE MRQGIRDKY ++KKE  P  D  L   EG+  R +KS        DP   S +      N+     +V E+P  ++T    ATDKC+L
Sbjct:   33 PEIAEAKREAEEKRNEKYRKMEEEREVMRQGIRDKYHIQKKE-LPKEDPGL---EGRLGRKKKSPDEAVEGGDPLQSSAQSSGFPKNLDDLTAKVKELPGTVMTTVSGATDKCNL 143
[Close]
BLAST of Complexin-1/2 vs. UniProt/SwissProt
Match: sp|Q8IPM8|CPLX_DROME (Complexin OS=Drosophila melanogaster OX=7227 GN=cpx PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.103e-13
Identity = 45/83 (54.22%), Postives = 57/83 (68.67%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAA---IRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENF 491
            MA FIAK +VG QL +VKGA+G  G ++ D K + + E       I+E E++R +KHRKMEEERE MRQ IRDKY +KKKE  
Sbjct:    1 MAAFIAKQMVGNQLSAVKGAVGGDGGDDGDDKEKAEEEERERQEAIKEAEDRRKEKHRKMEEEREKMRQDIRDKYNIKKKEEI 83
[Close]
BLAST of Complexin-1/2 vs. UniProt/SwissProt
Match: sp|Q4R4N1|CPLX1_MACFA (Complexin-1 OS=Macaca fascicularis OX=9541 GN=CPLX1 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 2.238e-7
Identity = 35/81 (43.21%), Postives = 46/81 (56.79%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKK--ENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSC--EDDTIIGNV 605
            A+R+ EE+R  K+ KME ERE MRQGIRDKYG+KKK             + EG   R +K+     GD    ED++I+  V
Sbjct:   40 ALRQAEEERKAKYAKMEAEREAMRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTV 120
[Close]
BLAST of Complexin-1/2 vs. TrEMBL
Match: A0A3Q0KFR3 (Complexin, putative OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 3.934e-34
Identity = 83/153 (54.25%), Postives = 104/153 (67.97%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXX---XXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKS--TLDPNGDSCEDDTIIG-----NVKGFANRVTEVPKQLVTEATDKCHLM 680
            MA+FIAK L+G QLDSVKGALGDK D        + EIDPE+EAA+RE EEKR  KHRKMEEERE MRQGIRDKYG+KKKE     D    S EG+  R RK+   L    +  +D+ I+      N++  AN+VTEVP ++++EA++KC LM
Sbjct:    1 MASFIAKQLIGNQLDSVKGALGDKKDEGEGVLGGRKEIDPEVEAALREAEEKREAKHRKMEEEREVMRQGIRDKYGIKKKEEEVPADFDFSSPEGRLGRKRKTPAELAAEANEADDENILTSMLPENMRNMANKVTEVPGKIISEASEKCLLM 153
[Close]
BLAST of Complexin-1/2 vs. TrEMBL
Match: A0A0X3PQ40 (Putative complexin-1 OS=Schistocephalus solidus OX=70667 GN=CPLX1 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.410e-33
Identity = 79/151 (52.32%), Postives = 103/151 (68.21%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXX-XXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKST--LDPNGDSC--EDDTIIG----NVKGFANRVTEVPKQLVTEATDKCHL 677
            MA FIAK L+G QL+SVKGALGDK D    S   +IDPE+E A+RE E++R +KHRKMEEERE MRQ IRDKYG+KKKE     +E +   EG+  R RK+   L    D     +D+I+G    NV+G AN+VTE P +L++EA++KC +
Sbjct:    1 MAGFIAKQLIGNQLESVKGALGDKKDEGGSSGAKQIDPEVEEALREAEQRRQEKHRKMEEEREVMRQDIRDKYGIKKKEEEINFEEIMMCNEGRLGRKRKTPAELAAEADKAAENEDSILGFLPENVRGIANKVTEAPSKLISEASEKCSI 151
[Close]
BLAST of Complexin-1/2 vs. TrEMBL
Match: A0A094ZLS3 (Putative complexin-1 OS=Schistosoma haematobium OX=6185 GN=MS3_03872 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 4.467e-33
Identity = 81/153 (52.94%), Postives = 102/153 (66.67%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXX---XXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSCED-------DTIIGNVKGFANRVTEVPKQLVTEATDKCHLM 680
            MA+FIAK L+G QLDSVKGALGDK D        + EIDPE+EAA+RE EEKR  KHRKMEEERE MRQGIRDKYG+KKKE     D    S EG+  R RK+  +   ++ E          +  N++  AN+VTEVP ++++EA++KC LM
Sbjct:    1 MASFIAKQLIGNQLDSVKGALGDKKDEGEGVLGGRKEIDPEVEAALREAEEKREAKHRKMEEEREVMRQGIRDKYGIKKKEEEVPADFDFSSPEGRLGRKRKTPAELAAEANEAEDENILTSMLPENMRNMANKVTEVPGKIISEASEKCLLM 153
[Close]
BLAST of Complexin-1/2 vs. TrEMBL
Match: A0A430PXQ8 (Complexin-1/2 OS=Schistosoma bovis OX=6184 GN=DC041_0002144 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 4.467e-33
Identity = 81/153 (52.94%), Postives = 102/153 (66.67%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXX---XXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSCED-------DTIIGNVKGFANRVTEVPKQLVTEATDKCHLM 680
            MA+FIAK L+G QLDSVKGALGDK D        + EIDPE+EAA+RE EEKR  KHRKMEEERE MRQGIRDKYG+KKKE     D    S EG+  R RK+  +   ++ E          +  N++  AN+VTEVP ++++EA++KC LM
Sbjct:    1 MASFIAKQLIGNQLDSVKGALGDKKDEGEGVLGGRKEIDPEVEAALREAEEKREAKHRKMEEEREVMRQGIRDKYGIKKKEEEVPADFDFSSPEGRLGRKRKTPAELAAEANEAEDENILTSMLPENMRNMANKVTEVPGKIISEASEKCLLM 153
[Close]
BLAST of Complexin-1/2 vs. TrEMBL
Match: A0A183P218 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS8404 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 4.467e-33
Identity = 81/153 (52.94%), Postives = 102/153 (66.67%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXX---XXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKSTLDPNGDSCED-------DTIIGNVKGFANRVTEVPKQLVTEATDKCHLM 680
            MA+FIAK L+G QLDSVKGALGDK D        + EIDPE+EAA+RE EEKR  KHRKMEEERE MRQGIRDKYG+KKKE     D    S EG+  R RK+  +   ++ E          +  N++  AN+VTEVP ++++EA++KC LM
Sbjct:    1 MASFIAKQLIGNQLDSVKGALGDKKDEGEGVLGGRKEIDPEVEAALREAEEKREAKHRKMEEEREVMRQGIRDKYGIKKKEEEVPADFDFSSPEGRLGRKRKTPAELAAEANEAEDENILTSMLPENMRNMANKVTEVPGKIISEASEKCLLM 153
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BLAST of Complexin-1/2 vs. Ensembl Cavefish
Match: CPLX1 (complexin 1 [Source:HGNC Symbol;Acc:HGNC:2309])

HSP 1 Score: 46.2098 bits (108), Expect = 4.637e-6
Identity = 21/33 (63.64%), Postives = 28/33 (84.85%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGL 473
            A+R+ EE+R  K+ KME ERE++RQGIRDKYG+
Sbjct:   40 ALRQQEEERKAKYAKMEAERESIRQGIRDKYGI 72
[Close]
BLAST of Complexin-1/2 vs. Ensembl Cavefish
Match: CPLX1 (complexin 1 [Source:HGNC Symbol;Acc:HGNC:2309])

HSP 1 Score: 45.8246 bits (107), Expect = 6.411e-6
Identity = 21/33 (63.64%), Postives = 28/33 (84.85%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGL 473
            A+R+ EE+R  K+ KME ERE++RQGIRDKYG+
Sbjct:   40 ALRQQEEERKAKYAKMEAERESIRQGIRDKYGI 72
[Close]
BLAST of Complexin-1/2 vs. Ensembl Medaka
Match: cplx2l (complexin-1 [Source:NCBI gene;Acc:110013827])

HSP 1 Score: 49.6766 bits (117), Expect = 2.794e-7
Identity = 24/36 (66.67%), Postives = 30/36 (83.33%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKK 482
            A+R+ EE+R  K+ KME ERE MRQGIRDKYG+KK+
Sbjct:   41 ALRQQEEERKAKYAKMEAEREVMRQGIRDKYGIKKR 76
[Close]
BLAST of Complexin-1/2 vs. Ensembl Medaka
Match: cplx2l (complexin-1 [Source:NCBI gene;Acc:110013827])

HSP 1 Score: 49.6766 bits (117), Expect = 2.794e-7
Identity = 24/36 (66.67%), Postives = 30/36 (83.33%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKK 482
            A+R+ EE+R  K+ KME ERE MRQGIRDKYG+KK+
Sbjct:   41 ALRQQEEERKAKYAKMEAEREVMRQGIRDKYGIKKR 76
[Close]
BLAST of Complexin-1/2 vs. Ensembl Medaka
Match: CPLX1 (complexin-1 [Source:NCBI gene;Acc:101172174])

HSP 1 Score: 48.1358 bits (113), Expect = 8.611e-7
Identity = 22/33 (66.67%), Postives = 28/33 (84.85%), Query Frame = 3 
Query:  375 AIRENEEKRLDKHRKMEEERENMRQGIRDKYGL 473
            A+R+ EE+R  K+ KME EREN+RQGIRDKYG+
Sbjct:   40 ALRQQEEERKAKYAKMEAERENLRQGIRDKYGI 72
[Close]
BLAST of Complexin-1/2 vs. Planmine SMEST
Match: SMESG000017115.1 (SMESG000017115.1)

HSP 1 Score: 152.91 bits (385), Expect = 4.523e-46
Identity = 90/151 (59.60%), Postives = 111/151 (73.51%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKS----TLDPNGDSCEDDTIIG----NVKGFANRVTEVPKQLVTEATDKCHLM 680
            MA+FIAK LVGKQLDSVKGALGDK   +++ K E+DPE+EAA++E E++RL+KHRKMEEERE MRQGIRDKYGLKKKE    MDE+   +  +  R RK+      D      E+D+I+G    NV+  AN+VTE PKQLV+EATDKC LM
Sbjct:    1 MASFIAKQLVGKQLDSVKGALGDKEGASSEEKKEVDPEVEAALKEAEDRRLEKHRKMEEERETMRQGIRDKYGLKKKEEASAMDEFGNPDGNRLGRKRKTPAELAADAAAAEEEEDSILGFLPPNVREMANKVTEAPKQLVSEATDKCKLM 151
[Close]
BLAST of Complexin-1/2 vs. Planmine SMEST
Match: SMESG000012002.1 (SMESG000012002.1)

HSP 1 Score: 114.005 bits (284), Expect = 5.777e-32
Identity = 71/72 (98.61%), Postives = 71/72 (98.61%), Query Frame = 3 
Query:  252 MATFIAKHLVGKQLDSVKGALXXXXXXXXXXXXEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKY 467
            MATFIAKHLVGKQLDSVKGALGDKGDNNNDSKNEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDK 
Sbjct:    1 MATFIAKHLVGKQLDSVKGALGDKGDNNNDSKNEIDPEIEAAIRENEEKRLDKHRKMEEERENMRQGIRDKV 72
The following BLAST results are available for this feature:
BLAST of Complexin-1/2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CPLX11.541e-741.98complexin 1 [Source:HGNC Symbol;Acc:HGNC:2309][more]
CPLX11.878e-741.98complexin 1 [Source:HGNC Symbol;Acc:HGNC:2309][more]
CPLX22.100e-763.41complexin 2 [Source:HGNC Symbol;Acc:HGNC:2310][more]
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BLAST of Complexin-1/2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cpx-19.640e-2145.26Putative complexin-1 [Source:UniProtKB/Swiss-Prot... [more]
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BLAST of Complexin-1/2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cpx2.408e-1455.56gene:FBgn0041605 transcript:FBtr0336482[more]
cpx3.095e-1454.22gene:FBgn0041605 transcript:FBtr0078901[more]
cpx3.095e-1454.22gene:FBgn0041605 transcript:FBtr0301769[more]
cpx3.095e-1454.22gene:FBgn0041605 transcript:FBtr0301771[more]
cpx3.095e-1454.22gene:FBgn0041605 transcript:FBtr0078899[more]
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BLAST of Complexin-1/2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cplx2l7.416e-845.71complexin 2, like [Source:ZFIN;Acc:ZDB-GENE-040718... [more]
cplx2l7.416e-845.71complexin 2, like [Source:ZFIN;Acc:ZDB-GENE-040718... [more]
cplx2l7.416e-845.71complexin 2, like [Source:ZFIN;Acc:ZDB-GENE-040718... [more]
cplx2l7.416e-845.71complexin 2, like [Source:ZFIN;Acc:ZDB-GENE-040718... [more]
cplx27.080e-663.64complexin 2 [Source:ZFIN;Acc:ZDB-GENE-081113-1][more]
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BLAST of Complexin-1/2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cplx16.799e-659.46complexin 1 [Source:NCBI gene;Acc:100124828][more]
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BLAST of Complexin-1/2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Cplx18.908e-843.21complexin 1 [Source:MGI Symbol;Acc:MGI:104727][more]
Cplx12.047e-765.00complexin 1 [Source:MGI Symbol;Acc:MGI:104727][more]
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BLAST of Complexin-1/2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9GUM7|CPLX1_CAEEL1.226e-1945.26Putative complexin-1 OS=Caenorhabditis elegans OX=... [more]
sp|Q60PP8|CPLX1_CAEBR1.339e-1845.99Putative complexin-1 OS=Caenorhabditis briggsae OX... [more]
sp|Q8I6U3|CPLX_HIRME7.762e-1646.09Complexin-1 OS=Hirudo medicinalis OX=6421 GN=cpx1 ... [more]
sp|Q8IPM8|CPLX_DROME3.103e-1354.22Complexin OS=Drosophila melanogaster OX=7227 GN=cp... [more]
sp|Q4R4N1|CPLX1_MACFA2.238e-743.21Complexin-1 OS=Macaca fascicularis OX=9541 GN=CPLX... [more]
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BLAST of Complexin-1/2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q0KFR33.934e-3454.25Complexin, putative OS=Schistosoma mansoni OX=6183... [more]
A0A0X3PQ401.410e-3352.32Putative complexin-1 OS=Schistocephalus solidus OX... [more]
A0A094ZLS34.467e-3352.94Putative complexin-1 OS=Schistosoma haematobium OX... [more]
A0A430PXQ84.467e-3352.94Complexin-1/2 OS=Schistosoma bovis OX=6184 GN=DC04... [more]
A0A183P2184.467e-3352.94Uncharacterized protein OS=Schistosoma mattheei OX... [more]
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BLAST of Complexin-1/2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CPLX14.637e-663.64complexin 1 [Source:HGNC Symbol;Acc:HGNC:2309][more]
CPLX16.411e-663.64complexin 1 [Source:HGNC Symbol;Acc:HGNC:2309][more]
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BLAST of Complexin-1/2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Complexin-1/2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Complexin-1/2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Complexin-1/2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cplx2l2.794e-766.67complexin-1 [Source:NCBI gene;Acc:110013827][more]
cplx2l2.794e-766.67complexin-1 [Source:NCBI gene;Acc:110013827][more]
CPLX18.611e-766.67complexin-1 [Source:NCBI gene;Acc:101172174][more]
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BLAST of Complexin-1/2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000017115.14.523e-4659.60SMESG000017115.1[more]
SMESG000012002.15.777e-3298.61SMESG000012002.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009438 ID=SMED30009438|Name=Complexin-1/2|organism=Schmidtea mediterranea sexual|type=transcript|length=893bp
ATTGACGTCTTCCTCTGACTATAAGAAAAGAGAAAATGCGTGTATATCTA
ACGAATACAATTTCAGCTTGCTACATCTAGAAATATATTTAATTCGGATT
TCTCGTGTTTGGACTCATTATAATTTGTTTTTTGATTTAATTGCCAGTTG
TTGTTGTTGATCTCTTACATTCTCGTTCTTTTATCTCAGATAATTTTGTG
ATTTTTCAGCATTCTCATCTATTCTTCACAAATTAGTACAGACTCCTTTC
GATGGCTACATTTATTGCAAAGCATTTGGTAGGTAAACAATTGGATTCAG
TTAAAGGTGCGTTGGGAGATAAAGGTGATAACAATAATGATAGCAAAAAT
GAAATTGACCCGGAAATCGAGGCTGCAATAAGAGAAAATGAGGAAAAAAG
ATTAGATAAACATCGCAAAATGGAAGAAGAAAGAGAAAATATGCGCCAAG
GGATACGTGACAAGTACGGGTTAAAGAAAAAAGAAAATTTCCCAATTATG
GATGAATATTTGGGAAGTGAAGAAGGAAAATGTAATAGAACTCGGAAAAG
CACTCTTGATCCCAATGGGGACTCTTGTGAGGATGATACGATAATTGGAA
ACGTGAAAGGTTTTGCGAACAGAGTCACTGAAGTACCAAAACAGTTGGTT
ACTGAAGCGACGGATAAGTGTCATTTAATGTAATGAAAAATTCTCATCCC
AATTTTCTATTGTTGACAATCTGTTTCCATAATACTTGTGTTGGATTTTA
ATGACAATGTCTATTGTTTACAATACATGTGAGTTTAGCGTGCTTCCTAT
TCTTTCTTTTGGATACAATGCTGAATTATGTGTGTCAAGTACTCCCCCCC
CCCGATACATACATACAAACGTGTAATGTCCTGAGTCTATTGG
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protein sequence of SMED30009438-orf-1

>SMED30009438-orf-1 ID=SMED30009438-orf-1|Name=SMED30009438-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=144bp
MATFIAKHLVGKQLDSVKGALGDKGDNNNDSKNEIDPEIEAAIRENEEKR
LDKHRKMEEERENMRQGIRDKYGLKKKENFPIMDEYLGSEEGKCNRTRKS
TLDPNGDSCEDDTIIGNVKGFANRVTEVPKQLVTEATDKCHLM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR008849Synaphin
Vocabulary: biological process
TermDefinition
GO:0006836neurotransmitter transport
Vocabulary: molecular function
TermDefinition
GO:0019905syntaxin binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 39..64
NoneNo IPR availableGENE3DG3DSA:1.20.5.580coord: 20..81
e-value: 2.9E-20
score: 74.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..72
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..72
NoneNo IPR availablePANTHERPTHR16705:SF4COMPLEXINcoord: 1..140
NoneNo IPR availableSUPERFAMILYSSF58038SNARE fusion complexcoord: 36..74
IPR008849SynaphinPFAMPF05835Synaphincoord: 2..107
e-value: 2.2E-30
score: 105.8
IPR008849SynaphinPANTHERPTHR16705COMPLEXINcoord: 1..140