bak-1

Overview
Namebak-1 (AEX93474.1)
Smed IDSMED30009339
Length (bp)998
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of bak-1 (SMED30009339) t-SNE clustered cells

Violin plots show distribution of expression levels for bak-1 (SMED30009339) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of bak-1 (SMED30009339) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for bak-1 (SMED30009339) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009339

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neoblastSMED30009339SMESG000011981.1 dd_Smed_v4_2588_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30009339SMESG000011981.1 dd_Smed_v4_2588_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of bak-1 vs. Ensembl Human
Match: BAK1 (BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:HGNC:949])

HSP 1 Score: 67.781 bits (164), Expect = 9.762e-13
Identity = 45/170 (26.47%), Postives = 80/170 (47.06%), Query Frame = 2 
Query:   62 KLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEI-VQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNN 568
            ++ ++T E+F  +V  + + ++E   +    P  P  ++   P    +   Q+G++LA I D +     +R   SE    L ++ P    A++ F++IA  +  + I W  V  L  FGY +A      G+  FL Q+ +++VD      I +WI   GGW+  L   N 
Sbjct:   26 QVAQDTEEVFRSYVFYRHQQEQEAEGV--AAPADP--EMVTLPLQPSSTMGQVGRQLAIIGDDI-----NRRYDSEFQTMLQHLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGFLGQVTRFVVDFMLHHCIARWIAQRGGWVAALNLGNG 186
[Close]
BLAST of bak-1 vs. Ensembl Human
Match: BAK1 (BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:HGNC:949])

HSP 1 Score: 61.2326 bits (147), Expect = 1.690e-10
Identity = 37/119 (31.09%), Postives = 58/119 (48.74%), Query Frame = 2 
Query:  215 QIGKKLAGIADTLVLDEESRGLISEI-VQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNN 568
            Q+G++LA I D +     +R   SE    L ++ P    A++ F++IA  +  + I W  V  L  FGY +A      G+  FL Q+ +++VD      I +WI   GGW+  L   N 
Sbjct:   53 QVGRQLAIIGDDI-----NRRYDSEFQTMLQHLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGFLGQVTRFVVDFMLHHCIARWIAQRGGWVAALNLGNG 166
[Close]
BLAST of bak-1 vs. Ensembl Xenopus
Match: rmdn1 (regulator of microtubule dynamics 1 [Source:Xenbase;Acc:XB-GENE-974332])

HSP 1 Score: 48.521 bits (114), Expect = 3.494e-6
Identity = 27/105 (25.71%), Postives = 48/105 (45.71%), Query Frame = 2 
Query:  257 LDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNNG 571
             +E  R   SEI   ++V P    A+  F+E+A  +    + W  +   F FG  +  + + + +   L ++  W+V   +   +  WI +NGGW  FL    +G
Sbjct:   48 FEERFRQAFSEISTQIHVTPGT--AYARFAEVAGSLFQGGVNWGRIVAFFVFGAALCAESVNKEMSPLLPRIQDWMVTYLE-TNLRDWIQSNGGWNGFLTLYGDG 149
[Close]
BLAST of bak-1 vs. Ensembl Mouse
Match: Bak1 (BCL2-antagonist/killer 1 [Source:MGI Symbol;Acc:MGI:1097161])

HSP 1 Score: 66.6254 bits (161), Expect = 1.906e-12
Identity = 45/173 (26.01%), Postives = 86/173 (49.71%), Query Frame = 2 
Query:   59 NKLCEETIEIFECFVETQLENDRE-RNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTL--VLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNN 568
             ++ ++T E+F  +V    + ++E +    P  P +  ++LP  P++   +  Q+G++LA I D +    D E + L+ ++       P    A++ F++IA  +  + I+W  V  L  FGY +A     +G+  FL Q+  ++ DI     I +WI   GGW+  L +  +
Sbjct:   23 QQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEM--DNLPLEPNS---ILGQVGRQLALIGDDINRRYDTEFQNLLEQL------QPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGFLGQVTCFLADIILHHYIARWIAQRGGWVAALNFRRD 184
[Close]
BLAST of bak-1 vs. UniProt/SwissProt
Match: sp|Q16611|BAK_HUMAN (Bcl-2 homologous antagonist/killer OS=Homo sapiens OX=9606 GN=BAK1 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 4.688e-12
Identity = 45/170 (26.47%), Postives = 80/170 (47.06%), Query Frame = 2 
Query:   62 KLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEI-VQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNN 568
            ++ ++T E+F  +V  + + ++E   +    P  P  ++   P    +   Q+G++LA I D +     +R   SE    L ++ P    A++ F++IA  +  + I W  V  L  FGY +A      G+  FL Q+ +++VD      I +WI   GGW+  L   N 
Sbjct:   26 QVAQDTEEVFRSYVFYRHQQEQEAEGV--AAPADP--EMVTLPLQPSSTMGQVGRQLAIIGDDI-----NRRYDSEFQTMLQHLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGFLGQVTRFVVDFMLHHCIARWIAQRGGWVAALNLGNG 186
[Close]
BLAST of bak-1 vs. UniProt/SwissProt
Match: sp|O08734|BAK_MOUSE (Bcl-2 homologous antagonist/killer OS=Mus musculus OX=10090 GN=Bak1 PE=1 SV=3)

HSP 1 Score: 66.6254 bits (161), Expect = 1.333e-11
Identity = 45/173 (26.01%), Postives = 86/173 (49.71%), Query Frame = 2 
Query:   59 NKLCEETIEIFECFVETQLENDRE-RNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTL--VLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNN 568
             ++ ++T E+F  +V    + ++E +    P  P +  ++LP  P++   +  Q+G++LA I D +    D E + L+ ++       P    A++ F++IA  +  + I+W  V  L  FGY +A     +G+  FL Q+  ++ DI     I +WI   GGW+  L +  +
Sbjct:   23 QQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEM--DNLPLEPNS---ILGQVGRQLALIGDDINRRYDTEFQNLLEQL------QPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGFLGQVTCFLADIILHHYIARWIAQRGGWVAALNFRRD 184
[Close]
BLAST of bak-1 vs. TrEMBL
Match: H2DL12 (Bak-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 394.43 bits (1012), Expect = 4.856e-136
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 2 
Query:   23 MAAGDVNNDDQMNKLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNNGDDTFXXXXXXXXXXXXXXXXXXXXYRNYSR 661
            MAAGDVNNDDQMNKLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNNGDDTFSVWVYRSAVVVVLAFVCISVYRNYSR
Sbjct:    1 MAAGDVNNDDQMNKLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNNGDDTFSVWVYRSAVVVVLAFVCISVYRNYSR 213
[Close]
BLAST of bak-1 vs. TrEMBL
Match: H2DL20 (Bcl2-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 4.577e-36
Identity = 67/152 (44.08%), Postives = 101/152 (66.45%), Query Frame = 2 
Query:   86 IFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGW 541
            IFEC++E  LE D+E N IDPGTPG+P +DL  T ++++  +  I +KL  IA  +  D +S  ++ +++ +VN+      AF++FSE+A++V+ N + W  +  LF FGY IARDRL QG + F   L +WI+DIF+R  +F WIL+ GGW
Sbjct:   17 IFECYLEDSLEKDKENNVIDPGTPGIPMDDL--TTNDKEAYA-IIVQKLLSIAGQM--DIKSDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 163
[Close]
BLAST of bak-1 vs. TrEMBL
Match: A0A1I8J8Q2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030130g1 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.617e-15
Identity = 51/163 (31.29%), Postives = 83/163 (50.92%), Query Frame = 2 
Query:   74 ETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLV------LDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIAN-EITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGW 541
            +T  +F+ FV  +   DRE+ R+ PG P  P   LP  P +  +V   IG++LA I D +        DE  + L  ++        D   +FD F  +A+++  +  I W  +  LF FGY IA +++ +GV  FL  +++WI     +  I +WI ++GGW
Sbjct:   66 QTDTVFQAFVFERYTRDREQGRLQPGAPSSPELILP--PADPLSVEAGIGRRLAEIGDEIQQRYQPEFDEMIKSLDGQLT-------DPASSFDTFRRVARRLFHDGSINWGRIVALFCFGYRIAVEKIRRGVSGFLRNVVQWIFRFLVQERIARWIADHGGW 219
[Close]
BLAST of bak-1 vs. TrEMBL
Match: A0A267FYD8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030621g2 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 7.517e-15
Identity = 51/163 (31.29%), Postives = 83/163 (50.92%), Query Frame = 2 
Query:   74 ETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLV------LDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIAN-EITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGW 541
            +T  +F+ FV  +   DRE+ R+ PG P  P   LP  P +  +V   IG++LA I D +        DE  + L  ++        D   +FD F  +A+++  +  I W  +  LF FGY IA +++ +GV  FL  +++WI     +  I +WI ++GGW
Sbjct:   90 QTDTVFQAFVFERYTRDREQGRLQPGAPSSPELILP--PADPLSVEAGIGRRLAEIGDEIQQRYQPEFDEMIKSLDGQLT-------DPASSFDTFRRVARRLFHDGSINWGRIVALFCFGYRIAVEKIRRGVSGFLRNVVQWIFRFLVQERIARWIADHGGW 243
[Close]
BLAST of bak-1 vs. TrEMBL
Match: A0A1S3HQ58 (bcl-2 homologous antagonist/killer-like OS=Lingula unguis OX=7574 GN=LOC106156464 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.215e-13
Identity = 50/171 (29.24%), Postives = 93/171 (54.39%), Query Frame = 2 
Query:   50 DQMNKLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIAN-EITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGY 559
            D  + + E+T  IF+ F+  +L++D+ +N  D  TP +P  +L +  D   + + QIG++LA I D +    E      +++Q + + PD   A++ F+ +A+K+  +  I W  + TL  FGY +A + L +G + F+ ++++++V       I +WI   GGW   L Y
Sbjct:   41 DTEDHVQEQTEGIFKNFIYQRLQDDQMKNPGDLETPFVP--ELTNFHDAPLSANAQIGRRLALIGDDINRKYEHE--FRDMIQALRITPDT--AYEAFAGVARKLFVDGRINWGRIITLLCFGYRMAIETLHRGFRSFVQKIVQFVVKFIAVEKIAKWIAEQGGWAAALRY 205
[Close]
BLAST of bak-1 vs. Ensembl Nematostella
Match: EDO49892 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF38])

HSP 1 Score: 65.4698 bits (158), Expect = 1.056e-12
Identity = 41/174 (23.56%), Postives = 85/174 (48.85%), Query Frame = 2 
Query:   44 NDDQMNKLCEETIEIFECFVETQLENDRERNRID---PGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQ-HFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFL 553
            ++D    +  ET  +F  F+  + +++ ER   +           E+L    D        IG++L  + D +  D + R    ++++ +++ P    A+D F+ +A+++  + I+W  +  L  FGY IA   + +G    FL ++++++VD   R  I +WI  +GGW+  +
Sbjct:   18 SEDSEKTVVSETEGVFRYFMYQRFQDEAERGDTEVQFTEARSDALEELRSCNDTSRQHIATIGRRLGEVGDEI--DAKYRDQFHDMIESLHLTPGT--AYDTFAAVARRLFRSGISWGRIIALLCFGYEIAITVIRRGFSGSFLRRIIRFVVDFIFRERIARWIAEHGGWVAVM 187
[Close]
BLAST of bak-1 vs. Ensembl Nematostella
Match: EDO42793 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0T4])

HSP 1 Score: 48.9062 bits (115), Expect = 1.261e-7
Identity = 35/110 (31.82%), Postives = 52/110 (47.27%), Query Frame = 2 
Query:  218 IGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIAN-EITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWI 544
            +   L  I D L  D     LI+E     +V  D   AFD F  +A ++  +  I W  + TLFYFGY +A   L Q     +  +++W+V   K   + +WI   GGW+
Sbjct:    1 VAHALREIGDQLTNDATLNNLIAE----CHVTKDT--AFDTFLAVALQIFQDGVINWGRIVTLFYFGYKLALKVLNQ--IPLIKMIIEWVVKFVKE-KLVKWIFQQGGWV 101
[Close]
BLAST of bak-1 vs. Ensembl Nematostella
Match: EDO37464 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFS9])

HSP 1 Score: 46.2098 bits (108), Expect = 3.651e-6
Identity = 33/127 (25.98%), Postives = 62/127 (48.82%), Query Frame = 2 
Query:  197 RDNVSWQIGKK----------LAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRL--GIFQWILNNGGW 541
            +D +S+++GK           L  +AD +  +E+   L++ +   +N+    + A+  F EIA +V A+ + W  +  L+ FG  +A        QH +  L++ + D   +   G+ +WI N GGW
Sbjct:   24 KDLISYRLGKNPKPPNANAETLRKLADKI--EEQYPALLNHLCHKLNI--SRSTAYSTFVEIAGEVFADGVNWGRIVVLYAFGGRLA----VYCEQHNMKDLVQSVTDWVGKYIAGLSEWIANEGGW 142
[Close]
BLAST of bak-1 vs. Ensembl Medaka
Match: zgc:153993 (apoptosis regulator BAX [Source:NCBI gene;Acc:101168687])

HSP 1 Score: 49.2914 bits (116), Expect = 2.400e-6
Identity = 31/126 (24.60%), Postives = 63/126 (50.00%), Query Frame = 2 
Query:  170 EDLPHTPDNRDNVSWQ-IGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWI 544
            EDL  +P+ +++   + + ++L  IAD +  +       + + QL+N     + A D F E+A+ + A+ I W  V  LF+  Y      LT      +  ++ W++ + K + ++ W++  GGW+
Sbjct:  146 EDLGGSPNEQEDPQIKEVVEQLLKIADDINRN-------AVLQQLINQA-SGSGAQDIFMEVARSIFADGINWGRVVALFHLAYKFIYKALTSNHLETIRMIISWVLQVIKEV-LYPWLVQQGGWV 262
[Close]
BLAST of bak-1 vs. Planmine SMEST
Match: SMESG000011981.1 (SMESG000011981.1)

HSP 1 Score: 394.43 bits (1012), Expect = 1.350e-139
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 2 
Query:   23 MAAGDVNNDDQMNKLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNNGDDTFXXXXXXXXXXXXXXXXXXXXYRNYSR 661
            MAAGDVNNDDQMNKLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNNGDDTFSVWVYRSAVVVVLAFVCISVYRNYSR
Sbjct:    1 MAAGDVNNDDQMNKLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGWIHFLGYNNNGDDTFSVWVYRSAVVVVLAFVCISVYRNYSR 213
[Close]
BLAST of bak-1 vs. Planmine SMEST
Match: SMESG000011963.1 (SMESG000011963.1)

HSP 1 Score: 143.28 bits (360), Expect = 2.345e-41
Identity = 71/157 (45.22%), Postives = 104/157 (66.24%), Query Frame = 2 
Query:   71 EETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGW 541
            EET  IFEC++E  LE D+E N IDPGTPG+P +DL  T ++++  +  I +KL  IA  +  D ES  ++ +++ +VN+      AF++FSE+A++V+ N + W  +  LF FGY IARDRL QG + F   L +WI+DIF+R  +F WIL+ GGW
Sbjct:   12 EETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDL--TTNDKEAYA-IIVQKLLSIAGQM--DIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 163
The following BLAST results are available for this feature:
BLAST of bak-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
BAK19.762e-1326.47BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:H... [more]
BAK11.690e-1031.09BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of bak-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rmdn13.494e-625.71regulator of microtubule dynamics 1 [Source:Xenbas... [more]
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BLAST of bak-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Bak11.906e-1226.01BCL2-antagonist/killer 1 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of bak-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q16611|BAK_HUMAN4.688e-1226.47Bcl-2 homologous antagonist/killer OS=Homo sapiens... [more]
sp|O08734|BAK_MOUSE1.333e-1126.01Bcl-2 homologous antagonist/killer OS=Mus musculus... [more]
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BLAST of bak-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2DL124.856e-136100.00Bak-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
H2DL204.577e-3644.08Bcl2-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV=... [more]
A0A1I8J8Q25.617e-1531.29Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FYD87.517e-1531.29Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1S3HQ586.215e-1329.24bcl-2 homologous antagonist/killer-like OS=Lingula... [more]
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BLAST of bak-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO498921.056e-1223.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427931.261e-731.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO374643.651e-625.98Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of bak-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1539932.400e-624.60apoptosis regulator BAX [Source:NCBI gene;Acc:1011... [more]
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BLAST of bak-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000011981.11.350e-139100.00SMESG000011981.1[more]
SMESG000011963.12.345e-4145.22SMESG000011963.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30009339
GenBankAEX93474.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009339 ID=SMED30009339|Name=bak-1|organism=Schmidtea mediterranea sexual|type=transcript|length=998bp
ATCTATATAAATCTTATATGTTATGGCAGCCGGTGATGTTAATAACGATG
ACCAAATGAATAAGCTATGCGAGGAAACTATTGAAATTTTCGAATGTTTT
GTTGAAACACAGTTGGAAAATGACCGTGAAAGAAATCGAATTGATCCCGG
TACACCCGGACTTCCACCAGAAGATTTGCCTCATACACCCGATAATAGAG
ATAATGTATCATGGCAAATCGGAAAGAAATTAGCTGGCATTGCGGATACT
TTAGTATTAGATGAAGAAAGTAGAGGATTAATTAGCGAAATAGTGCAATT
AGTAAATGTCGGACCAGATGACACTAGGGCATTTGATGAATTTTCGGAAA
TTGCTAAAAAAGTTATAGCTAATGAAATCACATGGGAAAATGTAGCGACC
TTGTTTTATTTTGGTTACTGCATAGCACGTGACCGATTGACACAAGGGGT
ACAGCATTTCCTGTCTCAGTTACTCAAGTGGATTGTTGATATTTTCAAGC
GGTTAGGAATATTTCAGTGGATTTTAAACAATGGAGGATGGATCCACTTT
CTAGGATATAATAACAACGGTGATGACACATTCAGTGTTTGGGTCTATCG
GTCCGCCGTTGTTGTGGTATTGGCCTTTGTTTGTATTTCAGTGTACCGAA
ATTATAGTCGTTAAACTTGTTTCCGATGGCTTTATATTATTTTTATATAA
TCAGGATACTCAAATTAAAATAAATTGCTGTTACAATGATCCTTGACTTC
TCATTTTTACACACACGCACACATAACAGCATGTACAATCTTCATTAATA
TGTCCGGTTTAGCCATTTACTATTTTACATTACCATTATTATTATTTTTA
CGATCAGGATTGGGATTTGAATGTGTAATTCAACAGTTTGAATTTTGACC
TATATTTTAATCAGAATATTCTTTTTATGTTAACATTTATTTTGTAATAA
TTTATCACTTTTTGTAATTTGAATATTTTGCTTATGTGTAAAAAAAAA
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protein sequence of SMED30009339-orf-1

>SMED30009339-orf-1 ID=SMED30009339-orf-1|Name=SMED30009339-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=214bp
MAAGDVNNDDQMNKLCEETIEIFECFVETQLENDRERNRIDPGTPGLPPE
DLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDD
TRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQL
LKWIVDIFKRLGIFQWILNNGGWIHFLGYNNNGDDTFSVWVYRSAVVVVL
AFVCISVYRNYSR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR036834Blc2-like_sf
IPR026298Blc2_fam
IPR002475Bcl2-like
Vocabulary: biological process
TermDefinition
GO:0042981regulation of apoptotic process
GO:0043065positive regulation of apoptotic process
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00337bclneu2coord: 71..174
e-value: 9.9E-4
score: 28.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 35..57
NoneNo IPR availablePANTHERPTHR11256:SF56coord: 10..207
NoneNo IPR availableTMHMMTMhelixcoord: 163..180
NoneNo IPR availableTMHMMTMhelixcoord: 190..212
IPR036834Blc2-like superfamilyGENE3DG3DSA:1.10.437.10coord: 7..183
e-value: 1.5E-28
score: 101.6
IPR036834Blc2-like superfamilySUPERFAMILYSSF56854Bcl-2 inhibitors of programmed cell deathcoord: 56..208
IPR026298Blc2 familyPFAMPF00452Bcl-2coord: 83..174
e-value: 1.1E-13
score: 51.7
IPR026298Blc2 familyPANTHERPTHR11256BCL-2 RELATEDcoord: 10..207
IPR002475Bcl2-likePROSITEPS50062BCL2_FAMILYcoord: 71..176
score: 12.2