SMED30009313
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009313 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30009313 aligns in the following genomic locations:
Homology
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050309.1 (SMESG000050309.1) HSP 1 Score: 878.626 bits (2269), Expect = 0.000e+0 Identity = 477/485 (98.35%), Postives = 480/485 (98.97%), Query Frame = 3 Query: 36 MNVIYKIFAFLMLIAIKINEKSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCSDIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQXXXXXXXXXXXXXXXXXXXXXKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQNDHVYYVISDQESNYHCL 1490 MNVIYKIFAFLMLIAIKINE SASYIYQEINQSLASVKQINFSMFMKNLNGVVLY VDHSDRILIELEKVNGVTEMV+TNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLV FQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCSDI+KLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQN+ VYYVI DQESNYHCL Sbjct: 1 MNVIYKIFAFLMLIAIKINENSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYGVDHSDRILIELEKVNGVTEMVFTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVRFQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCSDIQKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQNEPVYYVIMDQESNYHCL 485
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050309.1 (SMESG000050309.1) HSP 1 Score: 857.44 bits (2214), Expect = 0.000e+0 Identity = 466/474 (98.31%), Postives = 469/474 (98.95%), Query Frame = 3 Query: 69 MLIAIKINEKSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCSDIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQXXXXXXXXXXXXXXXXXXXXXKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQNDHVYYVISDQESNYHCL 1490 MLIAIKINE SASYIYQEINQSLASVKQINFSMFMKNLNGVVLY VDHSDRILIELEKVNGVTEMV+TNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLV FQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCSDI+KLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQN+ VYYVI DQESNYHCL Sbjct: 1 MLIAIKINENSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYGVDHSDRILIELEKVNGVTEMVFTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVRFQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCSDIQKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQNEPVYYVIMDQESNYHCL 474
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050303.1 (SMESG000050303.1) HSP 1 Score: 352.829 bits (904), Expect = 4.125e-117 Identity = 201/344 (58.43%), Postives = 250/344 (72.67%), Query Frame = 3 Query: 165 MFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKY---LNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCS-DIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKK 1184 MFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGC C IYMEWRIEL +R S+TC + Q L +S + I YP + Y N+T IMIGNIGPF T ++Y PL+NQLVGCL++LY+N ++ILFNS GS+FNSSC ++Y ETICGP YCN+S +TYKI DCSIS LNR+YC +DL QF + + +L AR+DW I+ L G CL++F+M++G FI FY Y + Y+ I+N+ I KL L SIETMV++F K++II+LSSGED++T+ IASEP IR+G K K CK F+GR+NFQ+K K+ Sbjct: 1 MFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCLECSYIYMEWRIELIKRRPSVTCGNVTQDLITVESRENA--NKILAYPLPKNYDTIRNQTFIMIGNIGPFEGNTGIVYNPLRNQLVGCLYKLYINGSRILFNSKGSIFNSSCFTDYTETICGPRYCNESSVTYKITDCSISVLNRRYCTSDLHQFFGNQSHYLNGNLQFARLDWLIDVLEGSSCLIEFEMENGYFIAFYTSYLNLYVDFIHNSRVHYHIPKL--LHAKSIETMVVEFTGKNVIIKLSSGEDIKTYSYPIASEPIFIRIGSFFKTKYCKTFQGRINFQKKMKQ 340
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050303.1 (SMESG000050303.1) HSP 1 Score: 348.206 bits (892), Expect = 2.541e-115 Identity = 199/342 (58.19%), Postives = 248/342 (72.51%), Query Frame = 3 Query: 171 MKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKY---LNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCS-DIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKK 1184 MKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGC C IYMEWRIEL +R S+TC + Q L +S + I YP + Y N+T IMIGNIGPF T ++Y PL+NQLVGCL++LY+N ++ILFNS GS+FNSSC ++Y ETICGP YCN+S +TYKI DCSIS LNR+YC +DL QF + + +L AR+DW I+ L G CL++F+M++G FI FY Y + Y+ I+N+ I KL L SIETMV++F K++II+LSSGED++T+ IASEP IR+G K K CK F+GR+NFQ+K K+ Sbjct: 1 MKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCLECSYIYMEWRIELIKRRPSVTCGNVTQDLITVESRENA--NKILAYPLPKNYDTIRNQTFIMIGNIGPFEGNTGIVYNPLRNQLVGCLYKLYINGSRILFNSKGSIFNSSCFTDYTETICGPRYCNESSVTYKITDCSISVLNRRYCTSDLHQFFGNQSHYLNGNLQFARLDWLIDVLEGSSCLIEFEMENGYFIAFYTSYLNLYVDFIHNSRVHYHIPKL--LHAKSIETMVVEFTGKNVIIKLSSGEDIKTYSYPIASEPIFIRIGSFFKTKYCKTFQGRINFQKKMKQ 338
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050303.1 (SMESG000050303.1) HSP 1 Score: 247.284 bits (630), Expect = 5.468e-77 Identity = 170/400 (42.50%), Postives = 244/400 (61.00%), Query Frame = 3 Query: 342 MEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKY---LNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCS-DIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLR---------IKNLQRQXXXXXXXXXXXXXXXXXXXXXKMLKQIFHKIIKMNYRIF-----AKRKSGGKNLENIGETHQNLFFRQLQNDHVYYVISDQESNYHC 1487 MEWRIEL +R S+TC + Q L +S + + I YP + Y N+T IMIGNIGPF T ++Y PL+NQLVGCL++LY+N ++ILFNS GS+FNSSC ++Y ETICGP YCN+S +TYKI DCSIS LNR+YC +DL QF + + +L AR+DW I+ L G CL++F+M++G FI FY Y + Y+ I+N+ I KL L SIETMV++F K++II+LSSGED++T+ IASEP IR+G K K CK F+GR+NFQ+K K+ + S +LL I + ++ ++ I + FL+L + KQI + K+ F K K + E++ E + + ++ +Y+V+SDQ+S +C Sbjct: 1 MEWRIELIKRRPSVTCGNVTQDLITVESRENASTNKILAYPLPKNYDTIRNQTFIMIGNIGPFEGNTGIVYNPLRNQLVGCLYKLYINGSRILFNSKGSIFNSSCFTDYTETICGPRYCNESSVTYKITDCSISVLNRRYCTSDLHQFFGNQSHYLNGNLQFARLDWLIDVLEGSSCLIEFEMENGYFIAFYTSYLNLYVDFIHNSRVHYHIPKL--LHAKSIETMVVEFTGKNVIIKLSSGEDIKTYSYPIASEPIFIRIGSFFKTKYCKTFQGRINFQKKMKQ----ITSKSLLTTASPEINSTIPSKSKRENLLIIGLILMAFLVLLILCAQKFKQILFQKSKLKLNFFNHLSSQKTKPCEEIPEHMEEIEEYTTYSHIRETSIYFVVSDQDSIPYC 394 The following BLAST results are available for this feature:
BLAST of SMED30009313 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30009313 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30009313 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30009313 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30009313 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009313 ID=SMED30009313|Name=SMED30009313|organism=Schmidtea mediterranea sexual|type=transcript|length=1523bpback to top protein sequence of SMED30009313-orf-1 >SMED30009313-orf-1 ID=SMED30009313-orf-1|Name=SMED30009313-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=486bp MNVIYKIFAFLMLIAIKINEKSASYIYQEINQSLASVKQINFSMFMKNLNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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