SMED30009313

Overview
NameSMED30009313
Smed IDSMED30009313
Length (bp)1523
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009313 (SMED30009313) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009313 (SMED30009313) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009313 (SMED30009313) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009313 (SMED30009313) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30009313

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30009313SMESG000050309.1 dd_Smed_v4_18399_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009313 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.012385:3478..17873 -7280
v31.020873:1995..9961 -5852
Homology
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050309.1 (SMESG000050309.1)

HSP 1 Score: 878.626 bits (2269), Expect = 0.000e+0
Identity = 477/485 (98.35%), Postives = 480/485 (98.97%), Query Frame = 3
Query:   36 MNVIYKIFAFLMLIAIKINEKSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCSDIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQXXXXXXXXXXXXXXXXXXXXXKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQNDHVYYVISDQESNYHCL 1490
            MNVIYKIFAFLMLIAIKINE SASYIYQEINQSLASVKQINFSMFMKNLNGVVLY VDHSDRILIELEKVNGVTEMV+TNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLV FQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCSDI+KLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQN+ VYYVI DQESNYHCL
Sbjct:    1 MNVIYKIFAFLMLIAIKINENSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYGVDHSDRILIELEKVNGVTEMVFTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVRFQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCSDIQKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQNEPVYYVIMDQESNYHCL 485          
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050309.1 (SMESG000050309.1)

HSP 1 Score: 857.44 bits (2214), Expect = 0.000e+0
Identity = 466/474 (98.31%), Postives = 469/474 (98.95%), Query Frame = 3
Query:   69 MLIAIKINEKSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCSDIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQXXXXXXXXXXXXXXXXXXXXXKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQNDHVYYVISDQESNYHCL 1490
            MLIAIKINE SASYIYQEINQSLASVKQINFSMFMKNLNGVVLY VDHSDRILIELEKVNGVTEMV+TNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLV FQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCSDI+KLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQN+ VYYVI DQESNYHCL
Sbjct:    1 MLIAIKINENSASYIYQEINQSLASVKQINFSMFMKNLNGVVLYGVDHSDRILIELEKVNGVTEMVFTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLFNSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVRFQCLLDFKMKDGSFIIFYIIYPHFYMRSIYNTTCSDIQKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQRQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKNLENIGETHQNLFFRQLQNEPVYYVIMDQESNYHCL 474          
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050303.1 (SMESG000050303.1)

HSP 1 Score: 352.829 bits (904), Expect = 4.125e-117
Identity = 201/344 (58.43%), Postives = 250/344 (72.67%), Query Frame = 3
Query:  165 MFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKY---LNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCS-DIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKK 1184
            MFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGC  C  IYMEWRIEL  +R S+TC +  Q L   +S   +    I  YP  + Y    N+T IMIGNIGPF   T ++Y PL+NQLVGCL++LY+N ++ILFNS GS+FNSSC ++Y ETICGP YCN+S +TYKI DCSIS LNR+YC +DL QF   +   +  +L  AR+DW I+ L G  CL++F+M++G FI FY  Y + Y+  I+N+     I KL  L   SIETMV++F  K++II+LSSGED++T+   IASEP  IR+G   K K CK F+GR+NFQ+K K+
Sbjct:    1 MFMKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCLECSYIYMEWRIELIKRRPSVTCGNVTQDLITVESRENA--NKILAYPLPKNYDTIRNQTFIMIGNIGPFEGNTGIVYNPLRNQLVGCLYKLYINGSRILFNSKGSIFNSSCFTDYTETICGPRYCNESSVTYKITDCSISVLNRRYCTSDLHQFFGNQSHYLNGNLQFARLDWLIDVLEGSSCLIEFEMENGYFIAFYTSYLNLYVDFIHNSRVHYHIPKL--LHAKSIETMVVEFTGKNVIIKLSSGEDIKTYSYPIASEPIFIRIGSFFKTKYCKTFQGRINFQKKMKQ 340          
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050303.1 (SMESG000050303.1)

HSP 1 Score: 348.206 bits (892), Expect = 2.541e-115
Identity = 199/342 (58.19%), Postives = 248/342 (72.51%), Query Frame = 3
Query:  171 MKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYNIYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKY---LNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCS-DIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKK 1184
            MKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGC  C  IYMEWRIEL  +R S+TC +  Q L   +S   +    I  YP  + Y    N+T IMIGNIGPF   T ++Y PL+NQLVGCL++LY+N ++ILFNS GS+FNSSC ++Y ETICGP YCN+S +TYKI DCSIS LNR+YC +DL QF   +   +  +L  AR+DW I+ L G  CL++F+M++G FI FY  Y + Y+  I+N+     I KL  L   SIETMV++F  K++II+LSSGED++T+   IASEP  IR+G   K K CK F+GR+NFQ+K K+
Sbjct:    1 MKNLNGVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCLECSYIYMEWRIELIKRRPSVTCGNVTQDLITVESRENA--NKILAYPLPKNYDTIRNQTFIMIGNIGPFEGNTGIVYNPLRNQLVGCLYKLYINGSRILFNSKGSIFNSSCFTDYTETICGPRYCNESSVTYKITDCSISVLNRRYCTSDLHQFFGNQSHYLNGNLQFARLDWLIDVLEGSSCLIEFEMENGYFIAFYTSYLNLYVDFIHNSRVHYHIPKL--LHAKSIETMVVEFTGKNVIIKLSSGEDIKTYSYPIASEPIFIRIGSFFKTKYCKTFQGRINFQKKMKQ 338          
BLAST of SMED30009313 vs. Planmine SMEST
Match: SMESG000050303.1 (SMESG000050303.1)

HSP 1 Score: 247.284 bits (630), Expect = 5.468e-77
Identity = 170/400 (42.50%), Postives = 244/400 (61.00%), Query Frame = 3
Query:  342 MEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKY---LNKTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQIXXXXXXXXXXXXCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHEREMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSXXXXXXXXPHFYMRSIYNTTCS-DIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAIASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLR---------IKNLQRQXXXXXXXXXXXXXXXXXXXXXKMLKQIFHKIIKMNYRIF-----AKRKSGGKNLENIGETHQNLFFRQLQNDHVYYVISDQESNYHC 1487
            MEWRIEL  +R S+TC +  Q L   +S   + +  I  YP  + Y    N+T IMIGNIGPF   T ++Y PL+NQLVGCL++LY+N ++ILFNS GS+FNSSC ++Y ETICGP YCN+S +TYKI DCSIS LNR+YC +DL QF   +   +  +L  AR+DW I+ L G  CL++F+M++G FI FY  Y + Y+  I+N+     I KL  L   SIETMV++F  K++II+LSSGED++T+   IASEP  IR+G   K K CK F+GR+NFQ+K K+    + S +LL          I +  ++  ++ I   +  FL+L     +  KQI  +  K+    F      K K   +  E++ E  +   +  ++   +Y+V+SDQ+S  +C
Sbjct:    1 MEWRIELIKRRPSVTCGNVTQDLITVESRENASTNKILAYPLPKNYDTIRNQTFIMIGNIGPFEGNTGIVYNPLRNQLVGCLYKLYINGSRILFNSKGSIFNSSCFTDYTETICGPRYCNESSVTYKITDCSISVLNRRYCTSDLHQFFGNQSHYLNGNLQFARLDWLIDVLEGSSCLIEFEMENGYFIAFYTSYLNLYVDFIHNSRVHYHIPKL--LHAKSIETMVVEFTGKNVIIKLSSGEDIKTYSYPIASEPIFIRIGSFFKTKYCKTFQGRINFQKKMKQ----ITSKSLLTTASPEINSTIPSKSKRENLLIIGLILMAFLVLLILCAQKFKQILFQKSKLKLNFFNHLSSQKTKPCEEIPEHMEEIEEYTTYSHIRETSIYFVVSDQDSIPYC 394          
The following BLAST results are available for this feature:
BLAST of SMED30009313 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009313 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000050309.10.000e+098.35SMESG000050309.1[more]
SMESG000050309.10.000e+098.31SMESG000050309.1[more]
SMESG000050303.14.125e-11758.43SMESG000050303.1[more]
SMESG000050303.12.541e-11558.19SMESG000050303.1[more]
SMESG000050303.15.468e-7742.50SMESG000050303.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009313 ID=SMED30009313|Name=SMED30009313|organism=Schmidtea mediterranea sexual|type=transcript|length=1523bp
CTACTAATGATCAAGATTTAACATAGAAATTCAAAATGAACGTTATTTAC
AAAATATTTGCATTTCTGATGCTAATCGCCATCAAAATAAATGAAAAATC
CGCCAGCTATATCTACCAAGAAATTAATCAAAGTTTGGCATCAGTTAAAC
AAATTAACTTTTCAATGTTCATGAAGAATTTGAACGGAGTCGTGCTGTAT
GCGGTTGATCATTCGGATCGGATCCTGATAGAATTGGAAAAGGTGAATGG
AGTGACAGAAATGGTTTACACAAATAACAAACATTCTATTCGAAGGAATA
TTCATAGCATTTGCGGATGCTGGAATTGCTATAATATTTACATGGAATGG
CGGATTGAACTGGCAATAAAGCGACTGTCAATCACTTGTGAAGATTTTAT
TCAGACATTGAATATATCACAATCTAACACTACAAGTCTCTCAGAAAGTA
TTTCGAATTATCCTCCACTAAGAAAGTACCTCAATAAAACTTTAATTATG
ATTGGAAACATTGGGCCTTTCGTTGATTATACGGAAATGATTTACCTTCC
ATTGAAAAACCAATTAGTTGGATGTCTTCACCAGCTTTATGTAAATGAGA
ACCAAATCCTATTCAATTCTAATGGATCCCTATTCAATTCCTCCTGTCCC
AGTGAATACAAGGAAACGATTTGTGGACCTAATTATTGTAACCAATCTAT
TATTACATATAAAATAAACGATTGTTCAATTTCCCAACTGAATAGAAAAT
ATTGCGCTACAGATTTGAAGCAATTTCATGAAAGGGAAATGAGACTAATT
GAAGAAAGCTTGATAATTGCAAGAATTGACTGGGAAATAAATCCCTTGGT
AGGTTTCCAATGTTTGTTAGATTTTAAAATGAAAGATGGGTCATTTATAA
TATTTTATATAATTTATCCACATTTTTACATGAGAAGCATCTACAATACC
ACTTGTAGTGACATTAAAAAATTGAAATCTCTTGATGGCAATTCCATTGA
AACAATGGTTCTCAAATTTCTTGATAAAGACATCATCATTCAGTTATCAA
GTGGTGAAGATGTTCAAACCCACATTCAAGCCATAGCAAGTGAACCCACC
TCAATTAGAGTTGGCTACTCCTCAAAATTAAAGGAATGTAAATTTTTCAA
GGGGAGGATGAATTTCCAAGAGAAAAGAAAGAAAAAAATAAATTTTTTAT
TTTCAGTAGCTCTTTTGCGTATCAAGAATCTTCAACGACAATTGATAATT
GTGACAATAATTTTTACAATTGGACTTTTCCTGCTGCTAACTTATTTTTA
TTTGAAAATGCTTAAACAGATATTTCACAAAATAATAAAAATGAATTATA
GAATATTTGCAAAACGGAAATCCGGTGGAAAAAACCTAGAAAATATTGGA
GAAACTCATCAGAACCTTTTTTTCCGCCAGCTTCAAAACGATCATGTTTA
CTATGTTATTTCGGACCAGGAATCAAATTATCATTGTTTATAATTCTTTT
ACTTTGTATTTTGTTGAAAGTTG
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protein sequence of SMED30009313-orf-1

>SMED30009313-orf-1 ID=SMED30009313-orf-1|Name=SMED30009313-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=486bp
MNVIYKIFAFLMLIAIKINEKSASYIYQEINQSLASVKQINFSMFMKNLN
GVVLYAVDHSDRILIELEKVNGVTEMVYTNNKHSIRRNIHSICGCWNCYN
IYMEWRIELAIKRLSITCEDFIQTLNISQSNTTSLSESISNYPPLRKYLN
KTLIMIGNIGPFVDYTEMIYLPLKNQLVGCLHQLYVNENQILFNSNGSLF
NSSCPSEYKETICGPNYCNQSIITYKINDCSISQLNRKYCATDLKQFHER
EMRLIEESLIIARIDWEINPLVGFQCLLDFKMKDGSFIIFYIIYPHFYMR
SIYNTTCSDIKKLKSLDGNSIETMVLKFLDKDIIIQLSSGEDVQTHIQAI
ASEPTSIRVGYSSKLKECKFFKGRMNFQEKRKKKINFLFSVALLRIKNLQ
RQLIIVTIIFTIGLFLLLTYFYLKMLKQIFHKIIKMNYRIFAKRKSGGKN
LENIGETHQNLFFRQLQNDHVYYVISDQESNYHCL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..23
score: 0.625
NoneNo IPR availableTMHMMTMhelixcoord: 404..426