60S ribosomal protein L18a

Overview
Name60S ribosomal protein L18a
Smed IDSMED30009283
Length (bp)653
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 60S ribosomal protein L18a (SMED30009283) t-SNE clustered cells

Violin plots show distribution of expression levels for 60S ribosomal protein L18a (SMED30009283) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 60S ribosomal protein L18a (SMED30009283) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 60S ribosomal protein L18a (SMED30009283) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30009283

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 18

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30009283SMESG000060163.1 SmedASXL_004223SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30009283SMESG000060163.1 dd_Smed_v4_437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009283SMESG000060163.1 Contig42419newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009283SMESG000060163.1 Contig42419uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
progenitor cellSMED30009283SMESG000060163.1 dd_Smed_v6_437_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30009283SMESG000060163.1 dd_Smed_v6_437_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30009283SMESG000060163.1 dd_Smed_v6_437_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 60S ribosomal protein L18a vs. Ensembl Human
Match: RPL18A (ribosomal protein L18a [Source:HGNC Symbol;Acc:HGNC:10311])

HSP 1 Score: 224.557 bits (571), Expect = 1.362e-74
Identity = 104/176 (59.09%), Postives = 130/176 (73.86%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LPT K   P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA+KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. Ensembl Human
Match: RPL18A (ribosomal protein L18a [Source:HGNC Symbol;Acc:HGNC:10311])

HSP 1 Score: 200.675 bits (509), Expect = 1.578e-65
Identity = 98/176 (55.68%), Postives = 119/176 (67.61%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LPT K   P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA+KCRR                       HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRP----------------------HKPRFTTKRPNTFF 154          
BLAST of 60S ribosomal protein L18a vs. Ensembl Human
Match: RPL18A (ribosomal protein L18a [Source:HGNC Symbol;Acc:HGNC:10311])

HSP 1 Score: 193.356 bits (490), Expect = 1.028e-62
Identity = 88/147 (59.86%), Postives = 110/147 (74.83%), Query Frame = 3
Query:  186 MRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA+KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 147          
BLAST of 60S ribosomal protein L18a vs. Ensembl Human
Match: RPL18A (ribosomal protein L18a [Source:HGNC Symbol;Acc:HGNC:10311])

HSP 1 Score: 185.652 bits (470), Expect = 1.012e-59
Identity = 84/140 (60.00%), Postives = 106/140 (75.71%), Query Frame = 3
Query:  186 MRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFAT 605
            MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA+KCRR  +K  HDS + FP+   + +  HKPRF T
Sbjct:    1 MRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTT 140          
BLAST of 60S ribosomal protein L18a vs. Ensembl Human
Match: RPL18A (ribosomal protein L18a [Source:HGNC Symbol;Acc:HGNC:10311])

HSP 1 Score: 160.229 bits (404), Expect = 8.218e-50
Identity = 73/127 (57.48%), Postives = 93/127 (73.23%), Query Frame = 3
Query:  246 QLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            +LKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA+KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:   11 KLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 137          
BLAST of 60S ribosomal protein L18a vs. Ensembl Celegans
Match: rpl-20 (60S ribosomal protein L18a [Source:UniProtKB/Swiss-Prot;Acc:O44480])

HSP 1 Score: 181.415 bits (459), Expect = 6.106e-58
Identity = 83/176 (47.16%), Postives = 122/176 (69.32%), Query Frame = 3
Query:  102 RAIG-TLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            +A+G TL E+ V GR++PTEKE     ++M+IFA + V AKSR+WYF+  L+++KK NGEI+  K++ +K P +VKN+G+W+KY SR+G HNMY+EYRD +V  AVTQ Y++M ARHRA+   IH++ VQ + A   +RA +K  HD+ + FP+   + K  +   F T+R  T F
Sbjct:    4 KALGETLNEYVVVGRKIPTEKEPVTPIWKMQIFATNHVIAKSRFWYFVSMLRRVKKANGEILSIKQVFEKNPGTVKNYGVWLKYDSRTGHHNMYREYRDTTVAGAVTQCYRDMGARHRAQADRIHILKVQTVKAEDTKRAGIKMFHDAKIRFPLPHRVTKRKNLSVFTTARQNTHF 179          
BLAST of 60S ribosomal protein L18a vs. Ensembl Fly
Match: RpL18A (gene:FBgn0010409 transcript:FBtr0086947)

HSP 1 Score: 209.92 bits (533), Expect = 3.888e-69
Identity = 97/176 (55.11%), Postives = 128/176 (72.73%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            MRA G LKE++V GR+LP+EKE     Y+MRIFAPD + AKSR+WYF+RQLKK KK  GEIV  K++++  P  +KNFGIW++Y SRSG HNMY+EYRDL+VG AVTQ Y++M ARHRAR  SI +I V  I A K RR H+K  HDS + FP+ + ++   ++  F+  +P+T F
Sbjct:    1 MRAKGLLKEYEVVGRKLPSEKEPQTPLYKMRIFAPDNIVAKSRFWYFLRQLKKFKKTTGEIVSIKQVYETSPVKIKNFGIWLRYDSRSGTHNMYREYRDLTVGGAVTQCYRDMGARHRARAHSIQIIKVDSIPAAKTRRVHVKQFHDSKIKFPLVQRVHHKGNRKLFSFRKPRTYF 176          
BLAST of 60S ribosomal protein L18a vs. Ensembl Zebrafish
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:394035])

HSP 1 Score: 220.32 bits (560), Expect = 4.288e-73
Identity = 102/176 (57.95%), Postives = 128/176 (72.73%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LP+ K   P  Y+MRIFAP+ V AKSR+WYF+ QL+KMKK +GEIV C  + +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  +I ++ VQ IAANKCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRLLPSVKNPAPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKASGEIVYCGLVFEKSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVQVIAANKCRRPAIKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. Ensembl Zebrafish
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:394035])

HSP 1 Score: 89.3521 bits (220), Expect = 4.044e-23
Identity = 40/64 (62.50%), Postives = 50/64 (78.12%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVEC 290
            M+A GTL+E+KV GR LP+ K   P  Y+MRIFAP+ V AKSR+WYF+ QL+KMKK +GEIV C
Sbjct:    1 MKASGTLREYKVVGRLLPSVKNPAPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKASGEIVYC 64          
BLAST of 60S ribosomal protein L18a vs. Ensembl Xenopus
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:549444])

HSP 1 Score: 223.016 bits (567), Expect = 5.057e-74
Identity = 102/176 (57.95%), Postives = 131/176 (74.43%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LPT K  +P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++++K P  VKNFGIW++Y SRSG HNMY+EYRDL+  AAVTQ Y++M ARHRAR  +I ++ V+ I A+KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVIGRSLPTPKAPSPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVYEKTPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTAAAVTQCYRDMGARHRARAHAIQIMKVEVIPASKCRRPAIKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. Ensembl Xenopus
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:549444])

HSP 1 Score: 214.542 bits (545), Expect = 8.255e-71
Identity = 98/170 (57.65%), Postives = 126/170 (74.12%), Query Frame = 3
Query:  117 LKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            L+E+KV GR LPT K  +P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++++K P  VKNFGIW++Y SRSG HNMY+EYRDL+  AAVTQ Y++M ARHRAR  +I ++ V+ I A+KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 LREYKVIGRSLPTPKAPSPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVYEKTPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTAAAVTQCYRDMGARHRARAHAIQIMKVEVIPASKCRRPAIKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 170          
BLAST of 60S ribosomal protein L18a vs. Ensembl Xenopus
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:549444])

HSP 1 Score: 156.762 bits (395), Expect = 1.182e-48
Identity = 71/127 (55.91%), Postives = 93/127 (73.23%), Query Frame = 3
Query:  246 QLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            +LKKMKK +GEIV C ++++K P  VKNFGIW++Y SRSG HNMY+EYRDL+  AAVTQ Y++M ARHRAR  +I ++ V+ I A+KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    5 KLKKMKKSSGEIVYCGQVYEKTPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTAAAVTQCYRDMGARHRARAHAIQIMKVEVIPASKCRRPAIKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 131          
BLAST of 60S ribosomal protein L18a vs. Ensembl Mouse
Match: Rpl18a (ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:1924058])

HSP 1 Score: 224.172 bits (570), Expect = 1.496e-74
Identity = 104/176 (59.09%), Postives = 129/176 (73.30%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LPT K   P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAAGKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. Ensembl Mouse
Match: Rpl18a (ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:1924058])

HSP 1 Score: 196.823 bits (499), Expect = 3.188e-63
Identity = 93/158 (58.86%), Postives = 114/158 (72.15%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGL 572
            M+A GTL+E+KV GR LPT K   P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA KCRR  +K  H S        GL
Sbjct:    1 MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAAGKCRRPAVKQFHVSDPECGADAGL 158          
BLAST of 60S ribosomal protein L18a vs. Ensembl Mouse
Match: Rpl18a (ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:1924058])

HSP 1 Score: 192.971 bits (489), Expect = 9.790e-63
Identity = 88/147 (59.86%), Postives = 109/147 (74.15%), Query Frame = 3
Query:  186 MRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAAGKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 147          
BLAST of 60S ribosomal protein L18a vs. Ensembl Mouse
Match: Rpl18a (ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:1924058])

HSP 1 Score: 151.754 bits (382), Expect = 5.751e-47
Identity = 69/108 (63.89%), Postives = 84/108 (77.78%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQ 422
            M+A GTL+E+KV GR LPT K   P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ
Sbjct:    1 MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQ 108          
BLAST of 60S ribosomal protein L18a vs. Ensembl Mouse
Match: Rpl18a (ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:1924058])

HSP 1 Score: 148.673 bits (374), Expect = 1.378e-45
Identity = 68/117 (58.12%), Postives = 86/117 (73.50%), Query Frame = 3
Query:  249 LKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRF 599
            LKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA KCRR  +K  HDS + FP+   + +  HKPRF
Sbjct:    7 LKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAAGKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRF 123          
BLAST of 60S ribosomal protein L18a vs. UniProt/SwissProt
Match: sp|Q90YU9|RL18A_ICTPU (60S ribosomal protein L18a OS=Ictalurus punctatus OX=7998 GN=rpl18a PE=2 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 4.299e-75
Identity = 104/176 (59.09%), Postives = 130/176 (73.86%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LP+ K   P  Y+MRIFAP+ V AKSR+WYF+ QL+KMKK NGE V C  +H++ P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  +I ++ VQ IAANKCRRA +K  HDS + FP+   + +  HKPRF T RPQT F
Sbjct:    1 MKASGTLREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVQVIAANKCRRAAIKQFHDSKIKFPLPHRVLRRQHKPRFTTRRPQTFF 176          
BLAST of 60S ribosomal protein L18a vs. UniProt/SwissProt
Match: sp|O57561|RL18A_SALSA (60S ribosomal protein L18a OS=Salmo salar OX=8030 GN=rpl18a PE=2 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 8.467e-75
Identity = 104/176 (59.09%), Postives = 129/176 (73.30%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LP+ K   P  Y+MRIFAP+ V AKSR+WYF+ QL+KMKK NGE V C  +H+K P  VKNFG+W++  SRSG HNMY+EYRDL+  AAVTQ Y++M ARHRAR  SIH++ VQEIAANKCRR  +K  HDS + FP+   + +  H PRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRLLPSVKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEKTPLKVKNFGVWLRNDSRSGTHNMYREYRDLTTSAAVTQCYRDMGARHRARAHSIHIMKVQEIAANKCRRPAIKQFHDSKIKFPLPHRVLRRQHNPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. UniProt/SwissProt
Match: sp|Q02543|RL18A_HUMAN (60S ribosomal protein L18a OS=Homo sapiens OX=9606 GN=RPL18A PE=1 SV=2)

HSP 1 Score: 224.557 bits (571), Expect = 6.540e-74
Identity = 104/176 (59.09%), Postives = 130/176 (73.86%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LPT K   P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA+KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. UniProt/SwissProt
Match: sp|Q3T003|RL18A_BOVIN (60S ribosomal protein L18a OS=Bos taurus OX=9913 GN=RPL18A PE=2 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 6.540e-74
Identity = 104/176 (59.09%), Postives = 130/176 (73.86%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LPT K   P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA+KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. UniProt/SwissProt
Match: sp|P62718|RL18A_RAT (60S ribosomal protein L18a OS=Rattus norvegicus OX=10116 GN=Rpl18a PE=2 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 1.046e-73
Identity = 104/176 (59.09%), Postives = 129/176 (73.30%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LPT K   P  Y+MRIFAP+ V AKSR+WYF+ QLKKMKK +GEIV C ++ +K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  SI ++ V+EIAA KCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAAGKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. TrEMBL
Match: U6J6K5 (60S ribosomal protein L18a OS=Echinococcus granulosus OX=6210 GN=EGR_03701 PE=3 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 4.532e-82
Identity = 116/176 (65.91%), Postives = 137/176 (77.84%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            MRA G LK F V GR+LPT+KE  P PY+MRIFAPD VTAKSR+WYFIRQLKKMKKGNGEIV CK ++ ++P +VKN+GIWI+Y SRSG HNMYKEYRDL+   AVTQLY+EM ARHRAR +SI VI V+ I  NKCRR H+   HD +L FP+   +N+ LH PRF T+RP T F
Sbjct:    1 MRASGKLKLFVVIGRKLPTDKEPKPVPYRMRIFAPDSVTAKSRFWYFIRQLKKMKKGNGEIVSCKRLYPRKPLTVKNYGIWIRYDSRSGTHNMYKEYRDLTEEGAVTQLYREMGARHRARATSIQVIKVEAIPDNKCRRPHITQFHDKNLKFPLPHRVNRRLHAPRFTTARPLTTF 176          
BLAST of 60S ribosomal protein L18a vs. TrEMBL
Match: A0A0R3U2J3 (60S ribosomal protein L18a OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS697 PE=3 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 6.575e-82
Identity = 117/176 (66.48%), Postives = 135/176 (76.70%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            MRA G LK F V GRRLPT K+  P PY+MRIFAPD VTAKSR+WYFIRQLKKMKKGNGEIV CK +H K+P +VKN+GIWI+Y SRSG HNMYKEYRDLS   AVTQ+Y+EM ARHRAR +SI VI V+ I  +KCRR H+   HD  L FP+   +N+ LH PRF T+RP T F
Sbjct:    1 MRASGKLKLFIVVGRRLPTAKDPKPVPYRMRIFAPDSVTAKSRFWYFIRQLKKMKKGNGEIVSCKRLHPKKPLTVKNYGIWIRYDSRSGTHNMYKEYRDLSEEGAVTQMYREMGARHRARATSIQVIKVEAIPDSKCRRPHVTQFHDKDLKFPLPHRVNRRLHAPRFTTARPLTTF 176          
BLAST of 60S ribosomal protein L18a vs. TrEMBL
Match: A0A0R3SPF7 (60S ribosomal protein L18a OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS6842 PE=3 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 5.775e-80
Identity = 114/176 (64.77%), Postives = 134/176 (76.14%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            MRA G LK + V GR+ PTEKE  P PY+MRIFAPD VTAKSR+WYFIRQLKKMKKG+GEIV CK +H K+P  V+N+GIWI+Y SRSG HNMYKEYRDL+   AVTQLY+EM ARHRAR +SI VI V+ I   KCRR H+   HD +L FP+   +N+ LH PRF T+RP T F
Sbjct:    1 MRASGKLKLYVVVGRKNPTEKEPKPVPYRMRIFAPDLVTAKSRFWYFIRQLKKMKKGSGEIVSCKRLHPKKPLVVRNYGIWIRYDSRSGTHNMYKEYRDLTEEGAVTQLYREMGARHRARATSIQVIKVEVIPDGKCRRPHITQFHDKNLKFPLPHRVNRQLHTPRFTTARPLTTF 176          
BLAST of 60S ribosomal protein L18a vs. TrEMBL
Match: A0A068Y5B4 (60S ribosomal protein L18a OS=Echinococcus multilocularis OX=6211 PE=3 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 8.658e-80
Identity = 114/176 (64.77%), Postives = 136/176 (77.27%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            MRA G LK F V GR+LPT+KE  P PY+MRIFAPD VTAKSR+WYFIRQLKKMKKG+GEIV CK ++ ++  +VKN+GIWI+Y SRSG HNMYKEYRDL+   AVTQLY+EM ARHRAR +SI VI V+ I  NKCRR H+   HD +L FP+   +N+ LH PRF T+RP T F
Sbjct:    1 MRASGKLKLFIVIGRKLPTDKEPKPVPYRMRIFAPDSVTAKSRFWYFIRQLKKMKKGSGEIVSCKRLYPRKSLTVKNYGIWIRYDSRSGTHNMYKEYRDLTEEGAVTQLYREMGARHRARATSIQVIKVEAIPDNKCRRPHITQFHDKNLKFPLPHRVNRRLHAPRFTTARPLTTF 176          
BLAST of 60S ribosomal protein L18a vs. TrEMBL
Match: C1LUV4 (60S ribosomal protein L18a OS=Schistosoma japonicum OX=6182 GN=RPL18A PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 1.109e-79
Identity = 114/182 (62.64%), Postives = 138/182 (75.82%), Query Frame = 3
Query:   81 F*IICKMRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            F I+C MRA G LK F V GR+LPT KE  P PY+MRIFAP+ V AKSR+WYFIR+LKKMKKG+GEI+ C+ IH ++P  VKNFGIW++Y SRSG HNMYKEYR L+  +AVTQLY+EM ARHRAR  SI VI V+ I A+KCRR ++   HDS L FP+   + + LH+PRF T RP TAF
Sbjct:    4 FSIVCDMRANGELKLFSVVGRKLPTTKEPKPVPYRMRIFAPNSVVAKSRFWYFIRKLKKMKKGSGEIISCERIHPRKPLMVKNFGIWLRYDSRSGTHNMYKEYRGLTEESAVTQLYREMGARHRARAESIQVIKVEAIPASKCRRPYITQFHDSKLKFPLPHRVARPLHRPRFTTRRPTTAF 185          
BLAST of 60S ribosomal protein L18a vs. Ensembl Cavefish
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:103034512])

HSP 1 Score: 222.631 bits (566), Expect = 4.174e-74
Identity = 102/176 (57.95%), Postives = 128/176 (72.73%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LP+ K   P  Y+MRIFAP+ V AKSR+WYF+ QL+KMKK +GE V C  +H+K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  +I ++ VQ IAANKCRR  +K  HDS + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGTLREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKASGETVYCGLVHEKTPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVQVIAANKCRRPAIKQFHDSKIKFPLPHRVLRQQHKPRFTTRRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. Ensembl Sea Lamprey
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:394035])

HSP 1 Score: 212.231 bits (539), Expect = 1.090e-70
Identity = 97/176 (55.11%), Postives = 126/176 (71.59%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A G LKE+K+ GRRLP+ K+ +P  Y+MRIFAP+ V AKSR+WYF  QLKK+KK  GEIV C  + +K P  +KNFGIW++  SRSG HNMY+EYRDL+   AVT  Y++M ARHRAR  SI ++ V+EI A+KCRR  +K  H+S + FP+   + +  HKPRF T RP T F
Sbjct:    1 MKASGALKEYKIIGRRLPSPKQVHPPLYRMRIFAPNHVVAKSRFWYFASQLKKLKKSAGEIVYCGRVSEKSPEKIKNFGIWLRCDSRSGTHNMYREYRDLTTAGAVTSCYRDMGARHRARAHSIQIMKVEEIEASKCRRPAIKQFHNSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. Ensembl Sea Lamprey
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:394035])

HSP 1 Score: 199.904 bits (507), Expect = 7.890e-66
Identity = 91/170 (53.53%), Postives = 121/170 (71.18%), Query Frame = 3
Query:  117 LKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            LKEFKV GRR+PT K+  P  Y+MRIFAP+ V AKSR+WYF   L+K+KK +GE+V C  + +K P  +KNFGIW++Y SRSG HNMY+EYRDL+   AVT  Y++M ARHRAR  SI ++ V+ I A+KCRR  +K  H+S + FP+   + +  H PRF+T RP T +
Sbjct:   13 LKEFKVIGRRVPTPKQPRPPLYRMRIFAPNHVVAKSRFWYFASVLRKLKKSSGEMVYCGTVSEKFPQRIKNFGIWLRYESRSGTHNMYREYRDLTTAGAVTACYRDMGARHRARAHSIQIMKVEVIPASKCRRPGIKQFHNSKIRFPLPHRVLRKQHSPRFSTRRPNTFY 182          
BLAST of 60S ribosomal protein L18a vs. Ensembl Yeast
Match: RPL20A (Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication [Source:SGD;Acc:S000004855])

HSP 1 Score: 168.703 bits (426), Expect = 6.415e-54
Identity = 85/171 (49.71%), Postives = 114/171 (66.67%), Query Frame = 3
Query:  117 LKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAAN-KCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
             KE++V GRRLPTE    P  ++MRIFA + V AKSRYWYF+++L K+KK +GEIV   +I++  P+ VKNFG+W++Y SRSG HNMYKE RD+S  AAV  LYQ+MAARHRAR  SIH++ V EI      +R ++K      L FP+   + K+     F+  RP T +
Sbjct:    3 FKEYQVIGRRLPTESVPEPKLFRMRIFASNEVIAKSRYWYFLQKLHKVKKASGEIVSINQINEAHPTKVKNFGVWVRYDSRSGTHNMYKEIRDVSRVAAVETLYQDMAARHRARFRSIHILKVAEIEKTADVKRQYVKQFLTKDLKFPLPHRVQKSTKT--FSYKRPSTFY 171          
BLAST of 60S ribosomal protein L18a vs. Ensembl Yeast
Match: RPL20B (Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication [Source:SGD;Acc:S000005839])

HSP 1 Score: 168.703 bits (426), Expect = 6.415e-54
Identity = 85/171 (49.71%), Postives = 114/171 (66.67%), Query Frame = 3
Query:  117 LKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAAN-KCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
             KE++V GRRLPTE    P  ++MRIFA + V AKSRYWYF+++L K+KK +GEIV   +I++  P+ VKNFG+W++Y SRSG HNMYKE RD+S  AAV  LYQ+MAARHRAR  SIH++ V EI      +R ++K      L FP+   + K+     F+  RP T +
Sbjct:    3 FKEYQVIGRRLPTESVPEPKLFRMRIFASNEVIAKSRYWYFLQKLHKVKKASGEIVSINQINEAHPTKVKNFGVWVRYDSRSGTHNMYKEIRDVSRVAAVETLYQDMAARHRARFRSIHILKVAEIEKTADVKRQYVKQFLTKDLKFPLPHRVQKSTKT--FSYKRPSTFY 171          
BLAST of 60S ribosomal protein L18a vs. Ensembl Nematostella
Match: EDO44391 (60S ribosomal protein L18a [Source:UniProtKB/TrEMBL;Acc:A7RW50])

HSP 1 Score: 210.69 bits (535), Expect = 8.296e-70
Identity = 96/176 (54.55%), Postives = 128/176 (72.73%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+  G LKEF+V GR +P +K   P  Y+MRIFAPD V A+S++WYFI QLK+MKK  GEIV C++I++K+P  +KNFGIW++Y SRSG HNMY+EYRDL+V  AVT  Y++MAARHRARG SI ++ V+ I A+K RR H+K MH+S + FP    + K+  K +F   RP T +
Sbjct:    1 MKVQGELKEFQVIGRLMPNKKLTVPPLYKMRIFAPDDVVARSKFWYFISQLKRMKKSQGEIVSCQQIYEKKPLQIKNFGIWLRYDSRSGTHNMYREYRDLTVSGAVTACYRDMAARHRARGYSIQIMKVEVIPASKARRPHVKQMHNSKIKFPFPHRIMKSQFKSKFVAKRPNTMY 176          
BLAST of 60S ribosomal protein L18a vs. Ensembl Medaka
Match: rpl18a (ribosomal protein L18a [Source:NCBI gene;Acc:101164966])

HSP 1 Score: 223.016 bits (567), Expect = 2.537e-74
Identity = 102/176 (57.95%), Postives = 129/176 (73.30%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            M+A GTL+E+KV GR LP+ K   P  Y+MRIFAP+ V AKSR+WYF+ QL+KMKK +GEIV C  +H+K P  VKNFGIW++Y SRSG HNMY+EYRDL+   AVTQ Y++M ARHRAR  +I ++ VQ IAANKCRR  +K  HDS + FP+   + +  H+PRF T RP T F
Sbjct:    1 MKASGTLREYKVIGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKASGEIVYCGLVHEKSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVQVIAANKCRRPAIKQFHDSKIKFPLPHRVLRRQHQPRFTTKRPNTFF 176          
BLAST of 60S ribosomal protein L18a vs. Planmine SMEST
Match: SMESG000060163.1 (SMESG000060163.1)

HSP 1 Score: 372.089 bits (954), Expect = 2.615e-133
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 3
Query:   99 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 626
            MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF
Sbjct:    1 MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQLKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDLSVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHLSFPIKRGLNKNLHKPRFATSRPQTAF 176          
The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L18a vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPL18A1.362e-7459.09ribosomal protein L18a [Source:HGNC Symbol;Acc:HGN... [more]
RPL18A1.578e-6555.68ribosomal protein L18a [Source:HGNC Symbol;Acc:HGN... [more]
RPL18A1.028e-6259.86ribosomal protein L18a [Source:HGNC Symbol;Acc:HGN... [more]
RPL18A1.012e-5960.00ribosomal protein L18a [Source:HGNC Symbol;Acc:HGN... [more]
RPL18A8.218e-5057.48ribosomal protein L18a [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl-206.106e-5847.1660S ribosomal protein L18a [Source:UniProtKB/Swis... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RpL18A3.888e-6955.11gene:FBgn0010409 transcript:FBtr0086947[more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl18a4.288e-7357.95ribosomal protein L18a [Source:NCBI gene;Acc:39403... [more]
rpl18a4.044e-2362.50ribosomal protein L18a [Source:NCBI gene;Acc:39403... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpl18a5.057e-7457.95ribosomal protein L18a [Source:NCBI gene;Acc:54944... [more]
rpl18a8.255e-7157.65ribosomal protein L18a [Source:NCBI gene;Acc:54944... [more]
rpl18a1.182e-4855.91ribosomal protein L18a [Source:NCBI gene;Acc:54944... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rpl18a1.496e-7459.09ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:... [more]
Rpl18a3.188e-6358.86ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:... [more]
Rpl18a9.790e-6359.86ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:... [more]
Rpl18a5.751e-4763.89ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:... [more]
Rpl18a1.378e-4558.12ribosomal protein L18A [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of 60S ribosomal protein L18a vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q90YU9|RL18A_ICTPU4.299e-7559.0960S ribosomal protein L18a OS=Ictalurus punctatus ... [more]
sp|O57561|RL18A_SALSA8.467e-7559.0960S ribosomal protein L18a OS=Salmo salar OX=8030 ... [more]
sp|Q02543|RL18A_HUMAN6.540e-7459.0960S ribosomal protein L18a OS=Homo sapiens OX=9606... [more]
sp|Q3T003|RL18A_BOVIN6.540e-7459.0960S ribosomal protein L18a OS=Bos taurus OX=9913 G... [more]
sp|P62718|RL18A_RAT1.046e-7359.0960S ribosomal protein L18a OS=Rattus norvegicus OX... [more]
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BLAST of 60S ribosomal protein L18a vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
U6J6K54.532e-8265.9160S ribosomal protein L18a OS=Echinococcus granulo... [more]
A0A0R3U2J36.575e-8266.4860S ribosomal protein L18a OS=Mesocestoides corti ... [more]
A0A0R3SPF75.775e-8064.7760S ribosomal protein L18a OS=Hymenolepis diminuta... [more]
A0A068Y5B48.658e-8064.7760S ribosomal protein L18a OS=Echinococcus multilo... [more]
C1LUV41.109e-7962.6460S ribosomal protein L18a OS=Schistosoma japonicu... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl18a4.174e-7457.95ribosomal protein L18a [Source:NCBI gene;Acc:10303... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl18a1.090e-7055.11ribosomal protein L18a [Source:NCBI gene;Acc:39403... [more]
rpl18a7.890e-6653.53ribosomal protein L18a [Source:NCBI gene;Acc:39403... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPL20A6.415e-5449.71Ribosomal 60S subunit protein L20A; homologous to ... [more]
RPL20B6.415e-5449.71Ribosomal 60S subunit protein L20B; homologous to ... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO443918.296e-7054.5560S ribosomal protein L18a [Source:UniProtKB/TrEM... [more]
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BLAST of 60S ribosomal protein L18a vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl18a2.537e-7457.95ribosomal protein L18a [Source:NCBI gene;Acc:10116... [more]
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BLAST of 60S ribosomal protein L18a vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000060163.12.615e-133100.00SMESG000060163.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009283 ID=SMED30009283|Name=60S ribosomal protein L18a|organism=Schmidtea mediterranea sexual|type=transcript|length=653bp
TATTATTCAGAATTTCGATTTTAATTCAAATAACCAAAATTATCTTTGTT
TAAATTTGTTTATTGTGATAATTAACAAAATTTTAAATAATTTGCAAAAT
GAGGGCTATTGGAACTTTGAAAGAATTTAAAGTAACAGGTAGAAGATTAC
CTACTGAAAAGGAACAAAATCCAACTCCTTACCAAATGAGAATCTTTGCT
CCTGATTTTGTAACTGCCAAAAGCCGTTATTGGTATTTTATTAGACAATT
GAAGAAAATGAAAAAAGGAAACGGTGAAATTGTTGAATGTAAAGAAATTC
ACGATAAGAGACCATCATCAGTCAAAAATTTCGGAATTTGGATTAAATAC
TACAGCAGAAGTGGTATTCACAATATGTACAAGGAATACAGAGATTTGTC
TGTTGGAGCTGCAGTTACTCAACTTTACCAAGAAATGGCTGCTCGTCATA
GAGCAAGAGGTAGTTCTATCCATGTCATCGATGTTCAGGAAATTGCTGCT
AACAAATGTAGACGTGCACATATGAAAGCCATGCACGATTCTCACCTCTC
TTTCCCGATCAAGAGAGGACTCAATAAGAATTTACACAAGCCAAGATTCG
CCACAAGCAGACCACAAACAGCTTTTTAAGTTACTAAATAAATATTGGAT
TTT
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protein sequence of SMED30009283-orf-1

>SMED30009283-orf-1 ID=SMED30009283-orf-1|Name=SMED30009283-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=177bp
MRAIGTLKEFKVTGRRLPTEKEQNPTPYQMRIFAPDFVTAKSRYWYFIRQ
LKKMKKGNGEIVECKEIHDKRPSSVKNFGIWIKYYSRSGIHNMYKEYRDL
SVGAAVTQLYQEMAARHRARGSSIHVIDVQEIAANKCRRAHMKAMHDSHL
SFPIKRGLNKNLHKPRFATSRPQTAF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000120progenitor cell
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR02887750S_L18Ae/Ribosomal_L18a/L20
IPR021138Ribosomal_L18a/L20_eukaryotes
IPR023573Ribosomal_L18a//L18Ae/LX
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR02113860S ribosomal protein L18a/ L20, eukaryotesPIRSFPIRSF002190Ribosomal_L18acoord: 2..176
e-value: 3.6E-69
score: 229.4
IPR023573Ribosomal protein 50S-L18Ae/60S-L20/60S-L18APFAMPF01775Ribosomal_L18Acoord: 7..130
e-value: 1.4E-51
score: 173.7
NoneNo IPR availableGENE3DG3DSA:3.10.20.10coord: 2..72
e-value: 1.8E-31
score: 109.6
NoneNo IPR availableGENE3DG3DSA:3.10.20.10coord: 74..148
e-value: 5.3E-35
score: 121.5
NoneNo IPR availablePANTHERPTHR10052:SF160S RIBOSOMAL PROTEIN L18Acoord: 4..176
NoneNo IPR availableSUPERFAMILYSSF160374RplX-likecoord: 72..147
IPR02887750S ribosomal protein L18Ae/60S ribosomal protein L20 and L18aPANTHERPTHR1005260S RIBOSOMAL PROTEIN L18Acoord: 4..176