Netrin-4
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009281 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 13Note: Hover over icons to view figure legend
Homology
BLAST of Netrin-4 vs. Ensembl Human
Match: LAMB1 (laminin subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:6486]) HSP 1 Score: 866.685 bits (2238), Expect = 0.000e+0 Identity = 550/1767 (31.13%), Postives = 870/1767 (49.24%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE--------------IKINLPP--------VSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKD----LPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLG-----------------ECL-NYANLVSSKKCDF-LNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT------NSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKN-TFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDI------------IISNFSEKLSRLKYYV------YNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG-------ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNP--LMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLL-LDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASN--SKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C P GDLLIGR L +STCGL E YC+V+ +K C IC+S++PY + SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FP I +++++C S +S I PS G V+F A + D Y + +N + ITN+R++FV+LHT GD +D IR KYYYA+ + ++RG C CYGHAS+C +G +V+ MV+G C C+HNTKG +CE C DFY+D PW PA+ ++N C+KC CN HS SCHFD VY + +SGGVCD+CQHNT +NCE+CK YY++ I C +C CD G++ IC + GL AG C CK NVEG CD CK GF+ + + GC C C +GT+ CDS TG CYCK VTG C C ++ LSN +++GC C C + G N +C SG+C C+ G +C+E++ +Y + L +E I+ +P V + Y F I +M+ +D I V+++ G+ S + L SRY+V+P C EK + + L +T ++ P + ++ + S V G CL N ++V + D N S + + + C+C+ GS ++ C+ GGQCQC PNV G+TC RCA ++GF GCK C+C L GS+N+ C TG+C C +GV + CD C PG+W FP C C+CN + CD TG+CLNC+ + G+NC+ C GYYGD G C+ C CP G S + + C T C+C GY G CD C + +FGN G+CQPC+C NID ++C+ +TG C KCL++T G +C+ C GY+G+ + C KCVCN++GT S Q C+ TG+C CLPNV+G CDRC + L+S GC PCNC+ + SCN+ TGQC C G G+ C+ C++ FWGDP C C C+ G QC+++ G C C G+ G CD C +G G P+C+ C C+ WD + + N T L + + +S V P + S+E+ + +K+I P + +I + +E +++++ + NS + ++ Q +++N+ + K D+ GA + + K + L +E + L++D++ E++ ++K ++ L++E++ + + + +T P CSE CGG C+ D+ R C G N + + LD+ +S + + +Q + ++ +++ K +E + + T+ + E NL+ +I+ +L SADL E++ + K E+ ++ L + IR R+ L+ V IL S + + ++ + A RA + + + + + +K ++ ++ + A ++G + E+E + + ++RI L+ + ++ + + + + ++ ++ + D K ++ K K N +K +E K+ L ++ K + +L+ D E K + + +++K L L+ ++ S
Sbjct: 30 CAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKK--CFICNSQDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWGVYRYFAYDCEASFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQN-LLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGFNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSISCHFDMAVYLATGNVSGGVCDDCQHNTMGRNCEQCKPFYYQHPERDIRDPNFCERCTCDPAGSQNEGICDSYTDFSTGLIAGQCRCKLNVEGEHCDVCKEGFYDLSSEDPFGCKSCACNPLGTIPGGNPCDSETGHCYCKRLVTGQHCDQCLPEHWGLSN-DLDGCRPCDC-DLGGALNNSCFAESGQCSCRPHMIGRQCNEVEPGYYFATLDHYLYEAEEANLGPGVSIVERQYIQDRIPSWTGAGFVRVPEGAYLEFFIDNIPYSME-YDILIRYEPQLPDHWEKAVITVQRPGRIPTSSRCGNTIPDDDNQVVSLSPGSRYVVLPRPVCFEKGTNYTVRLELPQYTSSDSDVESPYTLIDSLVLMPYCKSLDIFTVGGSGDGVVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALLHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPSGCKPCECHLQGSVNAFCNPVTGQCHCFQGVYARQCDRCLPGHWGFPSCQPCQCNGHADDCDPVTGECLNCQDYTMGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGYFGNPSEVGGSCQPCQCHNNIDTTDPEACDKETGRCLKCLYHTEGEHCQFCRFGYYGDALQQDCRKCVCNYLGTVQEHCNGSDCQ--CDKATGQCLCLPNVIGQNCDRCAPNTWQLASGTGCDPCNCNA-AHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDV--ECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDVIIAELTNRTHRFLEKAKALKISGVIGPYR--ETVDSVERKVSEIKDILAQSPAAEPLKNIGNLFEEAEKLIKDVTEMMAQVEVKLSDTTSQSNSTAKELDSLQTEAESLDNTVKELAEQLEFIKNSDIRGALDSITKYFQMSLEAEERVNASTTEPNSTVEQSALMRDRV----EDVMMERESQFKEKQEEQARLLDELAGKLQSLDLSAAAEMTCGTPPGASCSETECGGPNCRTDEGERKC-GGPGCGGLVTVAHNAWQKAMDLDQDVLSALAE--VEQLSKMVSEAKLRADEAKQSAE--DILLKTNATKEKMDKSNEELRNLIKQIRNFLTQDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVESLSQVEVILQHSAADIARAEMLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAI---KQADEDIQGTQNLLTSIESETAASEETLFNASQRISELERNVEELKRKAAQNSGEAEYIEKVVYTVKQSAE-DVKKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRS 1770
BLAST of Netrin-4 vs. Ensembl Human
Match: LAMB1 (laminin subunit beta 1 [Source:HGNC Symbol;Acc:HGNC:6486]) HSP 1 Score: 866.685 bits (2238), Expect = 0.000e+0 Identity = 550/1767 (31.13%), Postives = 870/1767 (49.24%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE--------------IKINLPP--------VSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKD----LPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLG-----------------ECL-NYANLVSSKKCDF-LNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT------NSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKN-TFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDI------------IISNFSEKLSRLKYYV------YNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG-------ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNP--LMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLL-LDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASN--SKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C P GDLLIGR L +STCGL E YC+V+ +K C IC+S++PY + SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FP I +++++C S +S I PS G V+F A + D Y + +N + ITN+R++FV+LHT GD +D IR KYYYA+ + ++RG C CYGHAS+C +G +V+ MV+G C C+HNTKG +CE C DFY+D PW PA+ ++N C+KC CN HS SCHFD VY + +SGGVCD+CQHNT +NCE+CK YY++ I C +C CD G++ IC + GL AG C CK NVEG CD CK GF+ + + GC C C +GT+ CDS TG CYCK VTG C C ++ LSN +++GC C C + G N +C SG+C C+ G +C+E++ +Y + L +E I+ +P V + Y F I +M+ +D I V+++ G+ S + L SRY+V+P C EK + + L +T ++ P + ++ + S V G CL N ++V + D N S + + + C+C+ GS ++ C+ GGQCQC PNV G+TC RCA ++GF GCK C+C L GS+N+ C TG+C C +GV + CD C PG+W FP C C+CN + CD TG+CLNC+ + G+NC+ C GYYGD G C+ C CP G S + + C T C+C GY G CD C + +FGN G+CQPC+C NID ++C+ +TG C KCL++T G +C+ C GY+G+ + C KCVCN++GT S Q C+ TG+C CLPNV+G CDRC + L+S GC PCNC+ + SCN+ TGQC C G G+ C+ C++ FWGDP C C C+ G QC+++ G C C G+ G CD C +G G P+C+ C C+ WD + + N T L + + +S V P + S+E+ + +K+I P + +I + +E +++++ + NS + ++ Q +++N+ + K D+ GA + + K + L +E + L++D++ E++ ++K ++ L++E++ + + + +T P CSE CGG C+ D+ R C G N + + LD+ +S + + +Q + ++ +++ K +E + + T+ + E NL+ +I+ +L SADL E++ + K E+ ++ L + IR R+ L+ V IL S + + ++ + A RA + + + + + +K ++ ++ + A ++G + E+E + + ++RI L+ + ++ + + + + ++ ++ + D K ++ K K N +K +E K+ L ++ K + +L+ D E K + + +++K L L+ ++ S
Sbjct: 54 CAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKK--CFICNSQDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWGVYRYFAYDCEASFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQN-LLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGFNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSISCHFDMAVYLATGNVSGGVCDDCQHNTMGRNCEQCKPFYYQHPERDIRDPNFCERCTCDPAGSQNEGICDSYTDFSTGLIAGQCRCKLNVEGEHCDVCKEGFYDLSSEDPFGCKSCACNPLGTIPGGNPCDSETGHCYCKRLVTGQHCDQCLPEHWGLSN-DLDGCRPCDC-DLGGALNNSCFAESGQCSCRPHMIGRQCNEVEPGYYFATLDHYLYEAEEANLGPGVSIVERQYIQDRIPSWTGAGFVRVPEGAYLEFFIDNIPYSME-YDILIRYEPQLPDHWEKAVITVQRPGRIPTSSRCGNTIPDDDNQVVSLSPGSRYVVLPRPVCFEKGTNYTVRLELPQYTSSDSDVESPYTLIDSLVLMPYCKSLDIFTVGGSGDGVVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALLHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPSGCKPCECHLQGSVNAFCNPVTGQCHCFQGVYARQCDRCLPGHWGFPSCQPCQCNGHADDCDPVTGECLNCQDYTMGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGYFGNPSEVGGSCQPCQCHNNIDTTDPEACDKETGRCLKCLYHTEGEHCQFCRFGYYGDALQQDCRKCVCNYLGTVQEHCNGSDCQ--CDKATGQCLCLPNVIGQNCDRCAPNTWQLASGTGCDPCNCNA-AHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDV--ECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDVIIAELTNRTHRFLEKAKALKISGVIGPYR--ETVDSVERKVSEIKDILAQSPAAEPLKNIGNLFEEAEKLIKDVTEMMAQVEVKLSDTTSQSNSTAKELDSLQTEAESLDNTVKELAEQLEFIKNSDIRGALDSITKYFQMSLEAEERVNASTTEPNSTVEQSALMRDRV----EDVMMERESQFKEKQEEQARLLDELAGKLQSLDLSAAAEMTCGTPPGASCSETECGGPNCRTDEGERKC-GGPGCGGLVTVAHNAWQKAMDLDQDVLSALAE--VEQLSKMVSEAKLRADEAKQSAE--DILLKTNATKEKMDKSNEELRNLIKQIRNFLTQDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVESLSQVEVILQHSAADIARAEMLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAI---KQADEDIQGTQNLLTSIESETAASEETLFNASQRISELERNVEELKRKAAQNSGEAEYIEKVVYTVKQSAE-DVKKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRS 1794
BLAST of Netrin-4 vs. Ensembl Human
Match: LAMB4 (laminin subunit beta 4 [Source:HGNC Symbol;Acc:HGNC:6491]) HSP 1 Score: 786.178 bits (2029), Expect = 0.000e+0 Identity = 541/1783 (30.34%), Postives = 859/1783 (48.18%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENV-VSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSA-DTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIR-NKYYYAISEWIIRGRCSCYGHASKCKAKE---GQV-QVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE----------------------IKINLP----PVSKSG---------------------YYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSC-----QSYQDTMQLIKDSRYIVIPNK-CLEKDLPIVLTVF---TIESNNKXXXXXXXXXXXXXXXXNSTK---TRKVLGE-----CLNYANLVSSK----KCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSK------WQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTS----LEQNMKSLKEIKKY--FPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG----ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSE---------FCGGVGCKPDKCYRHAPMNKYCMGKS-NQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSR---RIESVNLLI-KIKTYLNGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDT-----------------SYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFS-LKGNLFHMFKN----ENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C+P GDLL+GR + L ASSTCGL+ +++YC+++ + + KC ICDS+ PY + +SH IENV VS P +WWQS +G+ +VSI + E+ F+F+ L FKT+RPAAM +ERS DYG W + YF+ +C FP+I + + +++C S++S I PS GG V+ D S + N ++ D+ +TN+R+ F +LHT GD + N +KYYYA+ E I+RG C C GHAS+C+ + G V MV+G C CQHNT G +CE+CKDF+ D+PW PA N CR C CNSHSS CHFD Y S +SGGVC++CQHNTE ++C+RC+ +YR+ I+ C+ C CD GT IC+ + LG AG C+CKENVEG +CD+CK + + + GC C+C +G+L CD TG+C C S+VTG C C +GY+ L N +++GCS C C G+ N C +G+C C+ G C E ++ + L +E + + P PV+ +G +F I + D + ++ G C QS + L +R +++P CLE D+ + V+ ++ + ++ + + +NS + +++ L E C+ A+ + + C+ L IS + + CKC+ GS ++C+++GGQCQC P V G+ C+RC++ SY GC C C GS ++ C TG+C C V G+ CD C GY+ FP CH C CN+ + CD +TG C NC G + G NC+ C +GYYG+ S G C+ C CP SS + + C ++ C C +GY+G C +C T +GN CQPC C+ NID +SC+ TGEC +CL NT G NC+ C G++G+ +TC +C C+ G + +C+ TG CPCLPNV GL CDRC D ++NL GC C+CD P + + C+QLTGQC C+ G GK C+ C++ ++GDP C C CN G+ C+ + G+CRC+ G++G+ CD C +G + P C C C+ QWDH + + L +N++ +E VC + L N+ ++ I K+ FP G + + ++ + + R I+ N Q ++ ++ D + +KN+ + E ++LN +SE+ K Y +I+ +K N + +D L ++ + ++ + I N +C + CGG C K +R C G + L + L I++ ++Q N +I+ + E N ++L + G I+++ E++NL I K+K +L L EN+ +I N ++ + I S+ N+ + L K + + + C+ ++ R E+ + LLN K TLN++ Q Q AN+ + L N+ + KN EN+ + ++ + +R + K+ + + K + + A+ + ++ + V K Y L+ +++ + + L K L D + + + I D E K+Q L+ K +L +L+D++ +
Sbjct: 23 CNRGACHPTTGDLLVGRNTQLMASSTCGLSRAQKYCILSYLEG--EQKCFICDSRFPYDPYDQPNSHTIENVIVSFEPDREKKWWQSENGLDHVSIRLDLEALFRFSHLILTFKTFRPAAMLVERSTDYGHNWKVFKYFAKDCATSFPNITSGQAQGVGDIVCDSKYSDIEPSTGGEVVLKVLDPSFEIENP--YSPYIQDLVTLTNLRINFTKLHTLGDALLGRRQNDSLDKYYYALYEMIVRGSCFCNGHASECRPMQKMRGDVFSPPGMVHGQCVCQHNTDGPNCERCKDFFQDAPWRPAADLQDNACRSCSCNSHSSRCHFDMTTYLASGGLSGGVCEDCQHNTEGQHCDRCRPLFYRDPLKTISDPYACIPCECDPDGTISGGICVSHSDPALGSVAGQCLCKENVEGAKCDQCKPNHYGLSATDPLGCQPCDCNPLGSLPFLTCDVDTGQCLCLSYVTGAHCEECTVGYWGLGN-HLHGCSPCDCDIGGAYSN-VCSPKNGQCECRPHVTGRSCSEPAPGYFFAPLNFYLYEAEEATTLQGLAPLGSETFGQSPAVHVVLGEPVPGNPVTWTGPGFARVLPGAGLRFAVNNIPFPVDFTIAIHYETQSAADWTVQIVVNPPGGSEHCIPKTLQSKPQSFALPAATRIMLLPTPICLEPDVQYSIDVYFSQPLQGESHAHSHVLVDSLGLIPQINSLENFCSKQDLDEYQLHNCVEIASAMGPQVLPGACERLI--ISMSAKLHDGAVACKCHPQGSVGSSCSRLGGQCQCKPLVVGRCCDRCSTGSYDLGHHGCHPCHCHPQGSKDTVCDQVTGQCPCHGEVSGRRCDRCLAGYFGFPSCHPCPCNRFAELCDPETGSCFNCGGFTTGRNCERCIDGYYGNPSSGQPCRPCLCPDDPSSNQYFAHSCYQNLWSSDVICNCLQGYTGTQCGECSTGFYGNPRISGAPCQPCACNNNIDVTDPESCSRVTGECLRCLHNTQGANCQLCKPGHYGSALNQTCRRCSCHASGVSPMECPPGGGACLCDPVTGACPCLPNVTGLACDRCADGYWNLVPGRGCQSCDCD-PRTSQSSHCDQLTGQCPCKLGYGGKRCSECQENYYGDPP--GRCIPCDCNRAGTQKPICDPDTGMCRCREGVSGQRCDRCARGHSQEFPTCLQCHLCFDQWDHTISSLSKAVQGLMR----LAANMEDKRETLPVCEADFKDLRGNVSEIERILKHPVFPSGKFLKVKDYHDSVRR--------QIMQLNEQLKAVYEFQDLKDTIER--AKNEADLLLEDLQEEIDLQSSVLNASIADSSENIKKYYHISSSAEKKINETSSTINTSANTRNDLLTILDTLTSKGNLSLERLKQIKIPDIQILNEKVCGDPGNVPCVPLPCGGALCTGRKGHRK------CRGPGCHGSLTLSTNALQKAQEAKSIIRNLDKQVRGLKN--QIESISEQAEVSKNNALQLRE-KLGNIRNQSDSEEENINLFIKKVKNFL-----------LEENVPPEDIEKVANGVLDIHLPIPSQ-----NLTDELVKIQKHMQLCEDYRTDENRLNEEADGAQKLLVKAKAAEKAANILLNLDK---TLNQLQQ------AQITQGRANSTITQLTANITKIKKNVLQAENQTREMKSELELAKQRSGLEDGLSLLQTKLQRHQDHAVNAKVQAESAQHQAGSLEKEFVELKKQYAILQRKTST--TGLTKETLGKVKQLKDAAEKLAGDTEAKIRRITDLERKIQDLNLSRQAKADQLRILEDQVVA 1744
BLAST of Netrin-4 vs. Ensembl Human
Match: LAMB4 (laminin subunit beta 4 [Source:HGNC Symbol;Acc:HGNC:6491]) HSP 1 Score: 786.178 bits (2029), Expect = 0.000e+0 Identity = 541/1783 (30.34%), Postives = 859/1783 (48.18%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENV-VSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSA-DTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIR-NKYYYAISEWIIRGRCSCYGHASKCKAKE---GQV-QVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE----------------------IKINLP----PVSKSG---------------------YYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSC-----QSYQDTMQLIKDSRYIVIPNK-CLEKDLPIVLTVF---TIESNNKXXXXXXXXXXXXXXXXNSTK---TRKVLGE-----CLNYANLVSSK----KCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSK------WQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTS----LEQNMKSLKEIKKY--FPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG----ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSE---------FCGGVGCKPDKCYRHAPMNKYCMGKS-NQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSR---RIESVNLLI-KIKTYLNGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDT-----------------SYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFS-LKGNLFHMFKN----ENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C+P GDLL+GR + L ASSTCGL+ +++YC+++ + + KC ICDS+ PY + +SH IENV VS P +WWQS +G+ +VSI + E+ F+F+ L FKT+RPAAM +ERS DYG W + YF+ +C FP+I + + +++C S++S I PS GG V+ D S + N ++ D+ +TN+R+ F +LHT GD + N +KYYYA+ E I+RG C C GHAS+C+ + G V MV+G C CQHNT G +CE+CKDF+ D+PW PA N CR C CNSHSS CHFD Y S +SGGVC++CQHNTE ++C+RC+ +YR+ I+ C+ C CD GT IC+ + LG AG C+CKENVEG +CD+CK + + + GC C+C +G+L CD TG+C C S+VTG C C +GY+ L N +++GCS C C G+ N C +G+C C+ G C E ++ + L +E + + P PV+ +G +F I + D + ++ G C QS + L +R +++P CLE D+ + V+ ++ + ++ + + +NS + +++ L E C+ A+ + + C+ L IS + + CKC+ GS ++C+++GGQCQC P V G+ C+RC++ SY GC C C GS ++ C TG+C C V G+ CD C GY+ FP CH C CN+ + CD +TG C NC G + G NC+ C +GYYG+ S G C+ C CP SS + + C ++ C C +GY+G C +C T +GN CQPC C+ NID +SC+ TGEC +CL NT G NC+ C G++G+ +TC +C C+ G + +C+ TG CPCLPNV GL CDRC D ++NL GC C+CD P + + C+QLTGQC C+ G GK C+ C++ ++GDP C C CN G+ C+ + G+CRC+ G++G+ CD C +G + P C C C+ QWDH + + L +N++ +E VC + L N+ ++ I K+ FP G + + ++ + + R I+ N Q ++ ++ D + +KN+ + E ++LN +SE+ K Y +I+ +K N + +D L ++ + ++ + I N +C + CGG C K +R C G + L + L I++ ++Q N +I+ + E N ++L + G I+++ E++NL I K+K +L L EN+ +I N ++ + I S+ N+ + L K + + + C+ ++ R E+ + LLN K TLN++ Q Q AN+ + L N+ + KN EN+ + ++ + +R + K+ + + K + + A+ + ++ + V K Y L+ +++ + + L K L D + + + I D E K+Q L+ K +L +L+D++ +
Sbjct: 23 CNRGACHPTTGDLLVGRNTQLMASSTCGLSRAQKYCILSYLEG--EQKCFICDSRFPYDPYDQPNSHTIENVIVSFEPDREKKWWQSENGLDHVSIRLDLEALFRFSHLILTFKTFRPAAMLVERSTDYGHNWKVFKYFAKDCATSFPNITSGQAQGVGDIVCDSKYSDIEPSTGGEVVLKVLDPSFEIENP--YSPYIQDLVTLTNLRINFTKLHTLGDALLGRRQNDSLDKYYYALYEMIVRGSCFCNGHASECRPMQKMRGDVFSPPGMVHGQCVCQHNTDGPNCERCKDFFQDAPWRPAADLQDNACRSCSCNSHSSRCHFDMTTYLASGGLSGGVCEDCQHNTEGQHCDRCRPLFYRDPLKTISDPYACIPCECDPDGTISGGICVSHSDPALGSVAGQCLCKENVEGAKCDQCKPNHYGLSATDPLGCQPCDCNPLGSLPFLTCDVDTGQCLCLSYVTGAHCEECTVGYWGLGN-HLHGCSPCDCDIGGAYSN-VCSPKNGQCECRPHVTGRSCSEPAPGYFFAPLNFYLYEAEEATTLQGLAPLGSETFGQSPAVHVVLGEPVPGNPVTWTGPGFARVLPGAGLRFAVNNIPFPVDFTIAIHYETQSAADWTVQIVVNPPGGSEHCIPKTLQSKPQSFALPAATRIMLLPTPICLEPDVQYSIDVYFSQPLQGESHAHSHVLVDSLGLIPQINSLENFCSKQDLDEYQLHNCVEIASAMGPQVLPGACERLI--ISMSAKLHDGAVACKCHPQGSVGSSCSRLGGQCQCKPLVVGRCCDRCSTGSYDLGHHGCHPCHCHPQGSKDTVCDQVTGQCPCHGEVSGRRCDRCLAGYFGFPSCHPCPCNRFAELCDPETGSCFNCGGFTTGRNCERCIDGYYGNPSSGQPCRPCLCPDDPSSNQYFAHSCYQNLWSSDVICNCLQGYTGTQCGECSTGFYGNPRISGAPCQPCACNNNIDVTDPESCSRVTGECLRCLHNTQGANCQLCKPGHYGSALNQTCRRCSCHASGVSPMECPPGGGACLCDPVTGACPCLPNVTGLACDRCADGYWNLVPGRGCQSCDCD-PRTSQSSHCDQLTGQCPCKLGYGGKRCSECQENYYGDPP--GRCIPCDCNRAGTQKPICDPDTGMCRCREGVSGQRCDRCARGHSQEFPTCLQCHLCFDQWDHTISSLSKAVQGLMR----LAANMEDKRETLPVCEADFKDLRGNVSEIERILKHPVFPSGKFLKVKDYHDSVRR--------QIMQLNEQLKAVYEFQDLKDTIER--AKNEADLLLEDLQEEIDLQSSVLNASIADSSENIKKYYHISSSAEKKINETSSTINTSANTRNDLLTILDTLTSKGNLSLERLKQIKIPDIQILNEKVCGDPGNVPCVPLPCGGALCTGRKGHRK------CRGPGCHGSLTLSTNALQKAQEAKSIIRNLDKQVRGLKN--QIESISEQAEVSKNNALQLRE-KLGNIRNQSDSEEENINLFIKKVKNFL-----------LEENVPPEDIEKVANGVLDIHLPIPSQ-----NLTDELVKIQKHMQLCEDYRTDENRLNEEADGAQKLLVKAKAAEKAANILLNLDK---TLNQLQQ------AQITQGRANSTITQLTANITKIKKNVLQAENQTREMKSELELAKQRSGLEDGLSLLQTKLQRHQDHAVNAKVQAESAQHQAGSLEKEFVELKKQYAILQRKTST--TGLTKETLGKVKQLKDAAEKLAGDTEAKIRRITDLERKIQDLNLSRQAKADQLRILEDQVVA 1744
BLAST of Netrin-4 vs. Ensembl Human
Match: LAMB2 (laminin subunit beta 2 [Source:HGNC Symbol;Acc:HGNC:6487]) HSP 1 Score: 778.859 bits (2010), Expect = 0.000e+0 Identity = 461/1392 (33.12%), Postives = 709/1392 (50.93%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE-MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---------------------IEKPTYP-------------LEHLKEIKINLPPVSKSGYYNFVITTDTK-NMQFFDSFIIVLSIYHVGKSSCQSY----QDTMQ--LIKDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYAN-------------------LVSSK-----KCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKW-----QGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSS---------GVFMSNVKFPKEIDDV-----------CTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQ------QNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMD--NLINEMSKNVITFNGVTCNTITESNPLMC 3992
C + C P GDLL+GR L ASSTCGL + YC+V+ + KC +CDS+ P+ + HSHRI+NVV+ PQ WWQS +G+ V+I++ E+EF FT + FKT+RPAAM +ERS D+GRTW+ Y YFS +C DFP +P + ++V+C S +S I PS G V++ D Y ++ +N + ITN+RV RLHT GD +D IR KYYYA+ E ++RG C CYGHAS+C G E MV+G C C+HNT+G +CE+C+DFY D PW PA+ ++ CRKCEC+ H+ SCHFD VY S +SGGVCD CQHNT ++CE C+ +YR+ + C C CD +G++ C + LGL +G C CKE+V G +C +C+ GF+ + ++ GC +C C GT+ + CD ++G CYCK VTG C C G++ LS+ ++ GC C C G+ D + CD+ +G+C C+ G RC++++ ++ +E+ P L+ + ++ + V K+ Y+ ++ + + Q+ + +IV V S + D +Q L +RY++ PN CLE + L + + + P L+D R ++ E + + LV SK C L IS +T+ + + C+CN GS ++ CN GGQC C P V G+ C+ CA YGF GC+ C+C G+L+S C +G+CLCR G G CD C+ G W FP C C CN + C++ TG CL C+ ++ G +C+ C G++GD G C+ C CP G S ++ + C + C C+ GY+G C+ C HFG+ G CQ C CSGNID D+C+ TG+C +CL +T G +C C G+ G A ++C +C CN +GTN + Q C+ +G+CPCLPNV G CDRC F+NL+S GC PC C +P A +CN+ TGQC+C+AG G+ C+ C++ WGDP C C C+ G QC+R G C C+PG++G CD C +G G P C C C+ WD + + +L + G F S+ +E + S Q +++ +E+++ I +E L++L+ + + +FN + + + + + K + GA++ S+++ + ++E+++ L + R+ + + +D + ++ N+ + ++ + T ++T+ N L+C
Sbjct: 42 CSRGSCYPATGDLLVGRADRLTASSTCGLNGPQPYCIVSHLQD--EKKCFLCDSRRPFSARDNPHSHRIQNVVTSFAPQRRAAWWQSENGIPAVTIQLDLEAEFHFTHLIMTFKTFRPAAMLVERSADFGRTWHVYRYFSYDCGADFPGVPLAPPRHWDDVVCESRYSEIEPSTEGEVIYRVLDPAIPIPDPYSSRIQN-LLKITNLRVNLTRLHTLGDNLLDPRREIREKYYYALYELVVRGNCFCYGHASECAPAPGAPAHAEGMVHGACICKHNTRGLNCEQCQDFYRDLPWRPAEDGHSHACRKCECHGHTHSCHFDMAVYLASGNVSGGVCDGCQHNTAGRHCELCRPFFYRDPTKDLRDPAVCRSCDCDPMGSQDGGRCDSHDDPALGLVSGQCRCKEHVVGTRCQQCRDGFFGLSISDRLGCRRCQCNARGTVPGSTPCDPNSGSCYCKRLVTGRGCDRCLPGHWGLSH-DLLGCRPCDCDVGGALDPQ-CDEGTGQCHCRQHMVGRRCEQVQPGYFRPFLDHLIWEAEDTRGQVLDVVERLVTPGETPSWTGSGFVRLQEGQTLEFLVASVPKAMDYDLLLRLEPQVPEQWAELELIVQRPGPVPAHSLCGHLVPKDDRIQGTLQPHARYLIFPNPVCLEPGISYKLHLKLVRTGGSAQPETPYSGPGLLIDSLVLLPRVLVLEMFSGGDAAALERQATFERYQCHEEGLVPSKTSPSEACAPLL--ISLSTLIYNGALPCQCNPQGSLSSECNPHGGQCLCKPGVVGRRCDLCAPGYYGFGPTGCQACQCSHEGALSSLCEKTSGQCLCRTGAFGLRCDRCQRGQWGFPSCRPCVCNGHADECNTHTGACLGCRDHTGGEHCERCIAGFHGDPRLPYGGQCRPCPCPEGPGSQRHFATSCHQDEYSQQIVCHCRAGYTGLRCEACAPGHFGDPSRPGGRCQLCECSGNIDPMDPDACDPHTGQCLRCLHHTEGPHCAHCKPGFHGQAARQSCHRCTCNLLGTNPQQCPSPDQCHCDPSSGQCPCLPNVQGPSCDRCAPNFWNLTSGHGCQPCAC-HPSRARGPTCNEFTGQCHCRAGFGGRTCSECQELHWGDPGLQ--CHACDCDSRGIDTPQCHRFTGHCSCRPGVSGVRCDQCARGFSGIFPACHPCHACFGDWDRVVQDLAARTQRLEQRAQELQQTGVLGAFESSFWHMQEKLGIVQGIVGARNTSAASTAQLVEATEELRRE--------IGEATEHLTQLEADLTDVQDENFNANHALSGLERDRLALNLTLRQLDQHLDLLKHSNFLGAYD----SIRHAHSQSAEAERRANTSALAVPSPVSNSASARHRTEALMDAQKEDFNSKHMANQRALGKLSAHTHTL-SLTDINELVC 1410
BLAST of Netrin-4 vs. Ensembl Celegans
Match: lam-1 (LAMinin related. See also lmb [Source:UniProtKB/TrEMBL;Acc:V6CIX7]) HSP 1 Score: 926.776 bits (2394), Expect = 0.000e+0 Identity = 598/1816 (32.93%), Postives = 895/1816 (49.28%), Query Frame = 3
Query: 84 IIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPY--QRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQV------QVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYI-----------------------EKPTYPLEHL------------KEIKINLPPVSKSGYYNFVITTDTKN--MQFFDSFIIVLSIYHVGKSSCQSYQDT-----MQLIKDSRYI-VIPNKCLEKDLPIVLTV-FTIESNNKXXXXXXXX------------XXXXXXXXNSTKTRKVLGE-----CLNYANLVS------SKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCRE-GVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLI---FNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHS------CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWD----------HFLIVVKNTFDKLHNSSGV---FMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYN-SNIIDFNTQ-----QNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQ------KSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVG----CKPDKCYRHAPMNKYCMGKSNQF--FNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQ---SRRIESVNLLIKIKTYLNGS-ADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNI-STLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNN--------ISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKID----TKVVVNKSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYSKSEFHAQC 5120
+ A++ L+ ++++ C+ C P G+LLIGR+S L+A+STCG +R+C+V+ + ++KC CDS+ + QR SHRIENVV+ + D R W+QS +G NVSI E+EF FT + FK++RPAAM IERS D+G+TW Y YF+ +C FP IPE +V+CTS++S + PS GG +++ + V + Y ++ + + ITN+R F +LHT GD +D P I KYYYAI E ++RG CSCYGHAS+C + V + ++V+G C+C HNT+G +CEKCK FYND PW PA + NECR+C CN H+ CHFD VY+ S +SGGVCD+C HNT+ KNCE+CK +YR+ I+ CL C CD G++ IC E + + GL AG C CK NV+GN+CD+CK G+W GC+ C C ++GT N GCD TG C CK VTG +C C ++ LS +++GC C C GS DN TC+ +G+C C+ GF+G RC+ S +Y E P+ P E I +N P V S YN +I D + + + I V+ G SC + ++ SRYI V P CLE + L + F + N P + S + + L E C + A +S ++ C+ I+ + K+ +C C+ TGS + CN GGQC+C PNV G+ C++CA +YGF GCKKC CD GSL + C +G+C+CRE G+ G+ C+ C+PG+W FP C +C+CN + CD +G C+ C+ + G+ C C++GYYGD GI CK C CP G +S ++ C L NT C CK GY G+ C +C NH+G+ GTC+ C C+GNID +E SC++ TGEC KCL +T G CE CV GY+G+ ++TC +CVCN +GTNS +G C+ +G+CPC N++G+QCD+C + FNL+S GC C CD LNH CN GQC C+ GR G+ C+ C+D +WGDPT +GC +C CN GS QC+RNNG C C+ G G C+ C +G G+ P C+ CG+C+ QWD H LI N + +S F + KE + + + ++E+ K + +I+ EKL ++ + N + +DF + Q + + +T + SK K DV GAFN ++S L+ + + +++ +E L F +Q + + L+ + + N C S P C CGG G C C A + K+NQ F A LD KE + +I ++D + K +V + Q S R E + +I +L + + +L E + EI+ ++I L +I+ + K+NN++ IL++++ N+ + AV+A ++ L +++ I L Q + + V+ + + L +N EA + + + V +K L I + K T + K + ++ D T+++ + K+ ER LL ++ ND I L+K E++L + + SRL + I+ K++FHA C
Sbjct: 17 VFIEAQHPLQ-QNEDRCQDRSCYPITGNLLIGRKSQLKATSTCGSQGRQRFCIVSHLE--EQTKCFYCDSRTEWKPQREPYRLSHRIENVVTEVMDDKNRNWYQSENGAQNVSISFDLEAEFHFTHLIMTFKSFRPAAMIIERSADFGKTWQVYRYFAYDCDSSFPGIPEGPPKKHTDVICTSQYSDVAPSTGGEIVYKVISPHIVTENPYADEI-STLLKITNLRFNFTKLHTLGDDLLDYRPEIDEKYYYAIYEIVVRGSCSCYGHASRCIPIDPHVSPNTVMERADIVHGRCECMHNTEGLNCEKCKAFYNDLPWRPAIGDEKNECRQCNCNRHALRCHFDRAVYESSGFVSGGVCDDCMHNTQGKNCEQCKPFFYRDPRRTIDDPHVCLPCECDKAGSQNKGICEGEEDAERGLVAGKCYCKTNVDGNRCDRCKNGYWNLTETNVDGCVACTCNLLGTYNNEGCDKYTGMCTCKRLVTGENCDQCLPEHYGLSE-HVDGCKACDCDIGGSYDN-TCEITTGQCKCREGFSGRRCETADSSFYCADITHYVYEAEYANLTRGEVKTREWPSQPHEQTFTGEGFAQAVEGTIITVN-PIVEVSQKYNVIIRHDGARDPVGWENIQITVVRPEAEGDGSCSDAPPSDDFLIARIYPGSRYIEVQPAICLEAGVQYELRIQFNEKRGNSHPQERAAANILIDSILLAPPTSELHIFQGSARAEQHLTEYNRYQCRHLALSLSLFKDQRNEVCERYVCPIAAALLNKTS--ECNCDATGSVSGICNVQGGQCECKPNVVGRRCDQCAIGTYGFGPSGCKKCDCDAVGSLGNDCDKQSGQCVCREKGIYGRQCNQCQPGFWGFPECRTCQCNDHANICDQSSGACIECRDLTTGHYCDRCQDGYYGDPRLGVGIPCKPCPCPGGPTSGYQHADTCYLRNSGNNTQDIVCNCKSGYQGERCGECAQNHWGSPREVGGTCERCDCNGNIDMAMEGSCDAATGECLKCLHHTEGAQCEHCVDGYYGDAKLKTCQRCVCNELGTNST-KGACDRVSGQCPCHDNIIGMQCDQCAENHFNLASGAGCEACGCDSNGVVLNHEGVPHLQCNIFDGQCQCKPGRGGRKCDQCEDLYWGDPTTPDGCHRCECNPTGSKSLQCHRNNGTCECQAGSGGALCNECARGYTGQWPYCNPCGECFHQWDNIMQKLQKQVHALIDTANNIEDTGVASAYDADFEKMEETLKETKKALSDANISKEDIEEMSKKLALLKKQVIAG-REKLGAIETRISNITQAVDFAQKDLEHLQKEVDKVTKATIELEDKASKIKEADVLGAFNITRESASKSLDAQRRTDAAIGKLAAAENQALRASELLEKNKNDFEKQYVENEEALNEAETLLGGLESVLPKLNEQVCG--ASSAP--CDALCGGPGSCGFCGGQSCMEGA------VSKANQAKSFATEADTRLDEKQ-----KEAEEVLSI------VRDVLTETTKAKAKAEKAYEVAKNTAQRANSSRAELDKIAEEIGEFLTAQKSSPEQIRNLAEEVLGKEISLTPDQITDLTGKIKESLAKINNIDEILNETRGNKSIAANLESRAVKANKEAELLQKAMEEIREALQLADQAYNNVTS--VLEEIDTMQVQARELIDKARNSTEAVEGKAQAANTTLAELEGVMSGVKVEYLQISESAKNALTTVDAALAAATNAEQKNKQIQTDLERATELLEKRMEGNVAPQQRAEKLRERAAKLLYQAQRHNDDID------NLSKD---STEMRLDDYETILADLNSRLERVTRDIHEKTDFHATC 1789
BLAST of Netrin-4 vs. Ensembl Celegans
Match: lam-2 (Laminin-like protein lam-2 [Source:UniProtKB/Swiss-Prot;Acc:Q18823]) HSP 1 Score: 248.054 bits (632), Expect = 5.902e-66 Identity = 187/607 (30.81%), Postives = 282/607 (46.46%), Query Frame = 3
Query: 2370 CLCREGVEGQHCDTCKPGY-----WNFPFCH--SCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGT-CQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRT---CTKCVCNHMGT---NSKWQGI-CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSN------VKFPKEI---------------------------------DDVCTSLEQNMKSLKEIKKYFPIGYD----------IIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKN-----------ILNGASESK----------KIYQNITLIKDKLFYRNEEL-RNINLKFIRQKSKDMDNLINEMS 3926
C C G GQ C++C+ G+ + PF H C C+ S +C++++G C+ C+ N+ G C+ C GYYGD +G C+KC CP N C L + C GY+G+ CD+C +FGN KDGT C C CSGN D +C+ TGEC+KC+FNT GFNCE C GY+G+ + C C C GT N+ + + CN + G+C CLPNV+G+QCD+C F+N++S +GC CNCD P+G+ ++C+ TGQC C+ G +G+ C+ C D +G NGC C C GS +QC+ N+G C CK + GR CD C + G C C DC+ + V + L N+ + N KF +++ D++ +LE+ + S+ E +G D III N ++ + +Y ++ ++ + I S Y + SK +G E +K LK I N +K +I + I +K+K NE + R ++L ++KS D N + +S
Sbjct: 702 CECLPGFVGQFCESCESGFRRETKFGGPFNHCIKCDCHNHSNSCEAESGSCI-CEHNTAGDTCERCARGYYGDALQGTEEDCQKCPCP-------NDGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNP--KDGTECVECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDALIEPKGNCQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSGLGCQECNCD-PLGSEGNTCDVNTGQCQCKPGVTGQRCDRCADYHFG--FSANGCQPCDCEYIGSENQQCDVNSGQCLCKENVEGRRCDQCAENRYGITQGCLPCDDCYTLIQSRVNVFREKVKSLDNTLQEIIENPAPVNDTKFDEKVKETSRAASEVWEAVKQKTKEGGGTIKTKSKAIKDEIVAALEK-LTSIDESVAQAKVGADAAENDMKRWEIIIENARREIENVLHY------LETEGEERAQIAYNASQKYGEQ--SKRMSELASGTREEAEKHLKQASEIEQLSEQAIANATQANKEASDAIYGGEQISKQIAELKEKQNQLNESIHRTLDLAEEQKKSADEANNLAAVS 1286 HSP 2 Score: 205.297 bits (521), Expect = 8.242e-53 Identity = 152/482 (31.54%), Postives = 230/482 (47.72%), Query Frame = 3
Query: 192 LRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVH------NVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDF------PSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGS 1598
++ ++TCG ++CV + +G++S C CD + ++ FS + + V + WWQS + ++ + F T+ L+F + RP + I + W + ++S +C + P +P N CT EFS I P GG + FS + ++ + IR+ R++TFGD +V +P + YYYAIS++ + GRC C GHAS+C G V VC C+HNT+G C +C FYND PW NEC C C+ S+ C+FD +++++ GG C +CQ NT+ +CE+C + ++R C+ C C+ +G+ + C N G C CK V G CD+C GF+ F+ N GC C C G+L N+ CDSS+G C CK V G C CK GYF+LS N GC+ C C + S
Sbjct: 64 VQVTNTCGTKRPTKFCV-QSGHTGQRSVCETCDDR--HEGFSHPAKYLTDFNVG----NNETWWQSDTMQEGQQYPTTTNLTLVLGKSFDITYVRLKFISPRPESFTIYKKTHTDSEWEPWQFYSGSCRATYGLSDRAPILP----GNEATAQCTKEFSDISPITGGNIAFSTLEGRPSAHAFEESEVLQKWVTASAIRISLNRMNTFGD-EVFKDPQVLRSYYYAISDFAVGGRCKCNGHASECV---GSSSVDGENRLVCRCEHNTQGADCNECLPFYNDRPWRSGTSVEANECIACNCSQLSNRCYFDQQLFEETGH--GGHCIDCQGNTQGVHCEQCIANHWRRPG-----ENYCVACGCNEIGS-LSTQCD---------NEGKCQCKPGVTGRFCDQCLDGFYDFSTN---GCKNCGCETSGSLNNQPRCDSSSGSCSCKLNVEGRQCDKCKPGYFDLSTENQFGCTPCFCFGHSS 510 HSP 3 Score: 126.331 bits (316), Expect = 1.220e-28 Identity = 106/324 (32.72%), Postives = 154/324 (47.53%), Query Frame = 3
Query: 2163 IKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARG----CKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP----FCHSCKCNQMSTT-----CDSKTGKCLNCKGNSFGYNCQNCENGYYGDT---SRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCN 3086
IKC C+ + +N+C G C C N +G TCERCA YG +G C+KC C G D C G G+ CD C GY+ P C C C+ + CD TG+C C N+ G+NC+NC+ GY+GD +G C+ C C + + N+ L N C C G CDQC + F N S G CQ C C + ++C+ TG+C +C +G C++C +FG A C C C ++G+ ++ C+ +G+C C NV G +CD+C + + ++ GCLPC+
Sbjct: 734 IKCDCH---NHSNSCEAESGSCICEHNTAGDTCERCARGYYGDALQGTEEDCQKCPCPNDGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDALIEPKGN-CQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQC-AHGFYNITSGLG-CQECNCDPLGSEG--NTCDVNTGQC-QCKPGVTGQRCDRCADYHFGFSA-NGCQPCDCEYIGSENQQ---CDVNSGQCLCKENVEGRRCDQCAENRYGITQ--GCLPCD 1042 HSP 4 Score: 119.013 bits (297), Expect = 2.257e-26 Identity = 106/320 (33.12%), Postives = 138/320 (43.12%), Query Frame = 3
Query: 858 RCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFY------------------NDSP-WLPADKNST-NEC------RKC---------------ECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
+C C+ H++ C+A+ G C C+HNT G CE+C Y ND P L AD + EC R+C EC + S + DP K++G C C NT NCE CK GY+ + I C C C + GT+ P+ L+ G C C NV G QCD+C GF+ GC +CNC +G+ E CD +TG+C CK VTG C C +F S NGC C C EY +N+ CD SG+CLCK G RCD+ Y
Sbjct: 735 KCDCHNHSNSCEAESGS----------CICEHNTAGDTCERCARGYYGDALQGTEEDCQKCPCPNDGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGNTDPNSIGNCDKITG-ECKKCIFNTHGFNCENCKPGYWGD--ALIEPKGNCQSCGCFAAGTRRPNN--DYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSG--LGCQECNCDPLGS-EGNTCDVNTGQCQCKPGVTGQRCDRCADYHFGFS---ANGCQPCDC-EYIGSENQQCDVNSGQCLCKENVEGRRCDQCAENRY 1032 HSP 5 Score: 91.2781 bits (225), Expect = 6.844e-18 Identity = 59/156 (37.82%), Postives = 76/156 (48.72%), Query Frame = 3
Query: 2169 CKCNVTGSRNNN-------CNKIGGQCQCLPNVSGKTCERCASESYGF-DARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF--CHSCKCNQMST---TCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKC 2597
C C G+R N CN+ GQC CLPNV G C++CA Y GC++C CD GS + C +TG+C C+ GV GQ CD C ++ F C C C + + CD +G+CL CK N G C C YG T + C C
Sbjct: 890 CGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSGLGCQECNCDPLGSEGNTCDVNTGQCQCKPGVTGQRCDRCADYHFGFSANGCQPCDCEYIGSENQQCDVNSGQCL-CKENVEGRRCDQCAENRYGITQGCLPCDDC 1044 HSP 6 Score: 82.0333 bits (201), Expect = 4.054e-15 Identity = 83/296 (28.04%), Postives = 108/296 (36.49%), Query Frame = 3
Query: 939 CDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDS-----LGTKFPSICIQEPELDLGLNAGHCI---------------------------------------CKENVEGNQCDKCKIGFWKFNGNEETG-CLKCNCVMMGTLENRG------CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCD 1673
C+C GQ CE C+ + N C KC+C++HS+SC + G C C+HNT CERC GYY + + + C KC C + L IC + P G C C N G C+ CK G+W E G C C C GT C+ G+C C V G C C G++N+++ GC C C GS N TCD +G+C CK G G RCD
Sbjct: 702 CECLPGFVGQFCESCESGFRRETKFGG---PFNHCIKCDCHNHSNSC-----------EAESGSC-ICEHNTAGDTCERCARGYYGDALQG--TEEDCQKCPCPNDGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDALIEPKGNCQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGFYNITSG--LGCQECNCDPLGSEGN-TCDVNTGQCQCKPGVTGQRCD 977 HSP 7 Score: 77.411 bits (189), Expect = 1.114e-13 Identity = 78/258 (30.23%), Postives = 121/258 (46.90%), Query Frame = 3
Query: 2556 YYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGT------CQPCRCSG-----NIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHS--CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLN-GCTKCYCNLDGSVFKQCNRNNG 3287
YY S + +C+C G +S +G S + C C+ G C++C + + + GT C C CS DQ++ + G C C NT G +CE+C++ ++ C C CN +G+ S C++E GKC C P V G CD+C D F++ S+ GC C C+ G+LN+ C+ +G C C+ G+ C+ CK G++ T+ GCT C+C S+ CN +G
Sbjct: 274 YYYAISDFAVGGRCKCNGHAS---ECVGSSSVDGENRLVCRCEHNTQGADCNEC-LPFYNDRPWRSGTSVEANECIACNCSQLSNRCYFDQQLFEE-TGHGGHCIDCQGNTQGVHCEQCIANHWRRPGENYCVACGCNEIGSLSTQ---CDNE-GKCQCKPGVTGRFCDQCLDGFYDFSTN-GCKNCGCETS-GSLNNQPRCDSSSGSCSCKLNVEGRQCDKCKPGYFDLSTENQFGCTPCFCFGHSSI---CNTADG 517 HSP 8 Score: 72.4034 bits (176), Expect = 3.191e-12 Identity = 47/118 (39.83%), Postives = 67/118 (56.78%), Query Frame = 3
Query: 2163 IKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQ--CYNDTGKCLCREGVEGQHCDTCKPGYWNFPF-----CHSCKCNQMSTTCDSKTG 2495
+ C CN GS + C+ G+CQC P V+G+ C++C Y F GCK C C+ +GSLN+Q C + +G C C+ VEG+ CD CKPGY++ C C C S+ C++ G
Sbjct: 401 VACGCNEIGSLSTQCDN-EGKCQCKPGVTGRFCDQCLDGFYDFSTNGCKNCGCETSGSLNNQPRCDSSSGSCSCKLNVEGRQCDKCKPGYFDLSTENQFGCTPCFCFGHSSICNTADG 517 HSP 9 Score: 66.6254 bits (161), Expect = 2.018e-10 Identity = 67/218 (30.73%), Postives = 102/218 (46.79%), Query Frame = 3
Query: 2739 AISKDGTCQPCRCSGNIDQRIEDSCNSKTGECR---KCLFNTSGFNCEKCVSGY----FGNGA---VRTCTKCVCNHMGTNSKWQGICNHETGK----CPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQ--CNRNNGVCRCKPGITGRNCDMCMKG 3344
AIS C+C+G+ + + S S GE R +C NT G +C +C+ Y + +G C C C+ + + ETG C N G+ C++C + + C+ C C+ IG+L+ C+ G+C C+ G +G+ C+ C DGF+ T NGC C C GS+ Q C+ ++G C CK + GR CD C G
Sbjct: 277 AISDFAVGGRCKCNGHASECVGSS--SVDGENRLVCRCEHNTQGADCNECLPFYNDRPWRSGTSVEANECIACNCSQLSNRCYFDQQLFEETGHGGHCIDCQGNTQGVHCEQCIANHWRRPGENYCVACGCN-EIGSLSTQCDN-EGKCQCKPGVTGRFCDQCLDGFYDFST--NGCKNCGCETSGSLNNQPRCDSSSGSCSCKLNVEGRQCDKCKPG 488 HSP 10 Score: 60.8474 bits (146), Expect = 1.086e-8 Identity = 54/171 (31.58%), Postives = 80/171 (46.78%), Query Frame = 3
Query: 2217 GGQC-QCLPNVSGKTCERCASESYGFDARG-CKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF--CHSCKCNQMSTTCDSK----TGKCLNCKGNSFGYNCQNCENGYYG-DTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQ 2702
GG C C N G CE+C + + C C C+ GSL++QC N+ GKC C+ GV G+ CD C G+++F C +C C + + + +CK N G C C+ GY+ T C C C G SS+ N + G N ++ K+ ++GQ
Sbjct: 369 GGHCIDCQGNTQGVHCEQCIANHWRRPGENYCVACGCNEIGSLSTQCDNE-GKCQCKPGVTGRFCDQCLDGFYDFSTNGCKNCGCETSGSLNNQPRCDSSSGSCSCKLNVEGRQCDKCKPGYFDLSTENQFGCTPCFCFGHSSICNTADG-YFAMNVSSVFDQDKQKWAGQ 537 HSP 11 Score: 60.4622 bits (145), Expect = 1.458e-8 Identity = 49/155 (31.61%), Postives = 64/155 (41.29%), Query Frame = 3
Query: 2991 CPCLPNVVGLQCDRCQDKFFNLSSQIG-------CLPCNCDYPIGALNHSCNQLTGQCY-----------------CQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKG-TDGKMPNCSSCG 3380
C C N G C+ C + + + G C+ CNC +QL+ +CY CQ G C C W P + N C C CN GS+ QC+ N G C+CKPG+TGR CD C+ G D C +CG
Sbjct: 310 CRCEHNTQGADCNECLPFYNDRPWRSGTSVEANECIACNC-----------SQLSNRCYFDQQLFEETGHGGHCIDCQGNTQGVHCEQCIANHWRRPGE-NYCVACGCNEIGSLSTQCD-NEGKCQCKPGVTGRFCDQCLDGFYDFSTNGCKNCG 451 HSP 12 Score: 59.3066 bits (142), Expect = 3.220e-8 Identity = 60/211 (28.44%), Postives = 89/211 (42.18%), Query Frame = 3
Query: 2370 CLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTC---------DSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRC--SGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRT--CTKCVC 2933
C C +G C+ C P Y + P+ C +C C+Q+S C G C++C+GN+ G +C+ C ++ C C C + SL S C C CK G +G+FCDQC + S +G C+ C C SG+++ + +S + C+ N G C+KC GYF CT C C
Sbjct: 310 CRCEHNTQGADCNECLPFYNDRPWRSGTSVEANECIACNCSQLSNRCYFDQQLFEETGHGGHCIDCQGNTQGVHCEQCIANHWRRPGEN-YCVACGCNEIGSL---STQCD-----NEGKCQCKPGVTGRFCDQCLDGFYD--FSTNG-CKNCGCETSGSLNNQPRCDSSSGSCSCK---LNVEGRQCDKCKPGYFDLSTENQFGCTPCFC 505
BLAST of Netrin-4 vs. Ensembl Celegans
Match: unc-6 (Netrin unc-6 [Source:UniProtKB/Swiss-Prot;Acc:P34710]) HSP 1 Score: 207.223 bits (526), Expect = 5.043e-56 Identity = 133/438 (30.37%), Postives = 211/438 (48.17%), Query Frame = 3
Query: 123 DNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGR-KSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGV--HNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERK--RSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRL--------------HTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGF 1379
D+ R C P + G+ AS TCG ++YC V G + +C CD++N +Q ++ + ++ + W S + NVS+ +S +F+ T+ + F + P +M + +S D+G+TW + ++S C R F P+ +SN E +CT+ S I+ G V F + + + D T+I+V F RL +++G+ + DD ++ +Y+Y++ E + GRC C GHAS+C +M CDC+HNT G CE CK F+ D PW A NS N C C CN H+ C FD +++ S SGGVC NC+HNT +NC CK G+ R+ ++ + + C C C +G+ S C Q ++G C+CK V G C++C G+
Sbjct: 39 DHTGRPVRCVPEFINAAFGKPVI--ASDTCGTNRPDKYCTVKEGPDGIIREQCDTCDARNHFQSHPASLLTDLNSIGN------MTCWVSTPSLSPQNVSLTLSLGKKFELTYVSMHFCSRLPDSMALYKSADFGKTWTPFQFYSSECRRIFGRDPDVSITKSNEQEAVCTA--SHIMGPGGNRVAFPFLENRPSAQNFENSPVLQDWVTATDIKVVFSRLSPDQAELYGLSNDVNSYGN-ETDDE--VKQRYFYSMGELAVGGRCKCNGHASRCI-------FDKMGRYTCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKS-CNQ--------SSGQCVCKPGVTGTTCNRCAKGY 447 HSP 2 Score: 75.8702 bits (185), Expect = 1.836e-13 Identity = 57/172 (33.14%), Postives = 80/172 (46.51%), Query Frame = 3
Query: 1047 KCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNI--AVAINSSQTCLKCHCDSLGTKFPSICIQEPEL---DLGLNAGHCI-CKENVEGNQCDKCKIGFWK---FNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGY 1535
+C+CN H+S C FD KM CD C+HNT CE CK +Y NS+ +C+ C+C+ + C + EL + G C+ C+ N G C CK GF + C C C +G+L + C+ S+G+C CK VTG C+ C GY
Sbjct: 290 RCKCNGHASRCIFD--------KMGRYTCD-CKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKR----CRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSL-GKSCNQSSGQCVCKPGVTGTTCNRCAKGY 447 HSP 3 Score: 62.3882 bits (150), Expect = 2.667e-9 Identity = 50/179 (27.93%), Postives = 75/179 (41.90%), Query Frame = 3
Query: 2769 CRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVR-------TCTKCVCNHMGTNSKWQGIC-----NHETGKCP-CLPNVVGLQCDRCQDKF-----FNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLD 3251
C+C+G+ + I D T +C+ NT+G CE C ++ R +C C CN ++ N G C C N G C C+ F ++ + C C C +P+G+L SCNQ +GQC C+ G +G CN C G+ + + C K D
Sbjct: 291 CKCNGHASRCIFDKMGRYTCDCKH---NTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGC-HPVGSLGKSCNQSSGQCVCKPGVTGTTCNRCAKGYQQSRSTVTPCIKIPTKAD 465 HSP 4 Score: 62.3882 bits (150), Expect = 2.736e-9 Identity = 48/171 (28.07%), Postives = 77/171 (45.03%), Query Frame = 3
Query: 2889 YFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFN-------LSSQIGCLPCNCD-------YPIGALNHSCNQLTGQCY-CQAGRSGKMCNNCKDGFWGDP----TKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKG 3344
++ G + +C CN + + + + C C N G +C+ C+ ++ +S C+ CNC+ + S N+ G C C+ +G+ C+ CK GF D T C C C+ GS+ K CN+++G C CKPG+TG C+ C KG
Sbjct: 279 FYSMGELAVGGRCKCNGHASRCIFDKMGRY---TCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKSCNQSSGQCVCKPGVTGTTCNRCAKG 446 HSP 5 Score: 62.003 bits (149), Expect = 3.659e-9 Identity = 57/216 (26.39%), Postives = 90/216 (41.67%), Query Frame = 3
Query: 2310 KCKCDLTGSLNSQCYNDTGK--CLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTC-----------DSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEK 2876
+CKC+ G + ++ G+ C C+ G C+ CKP +++ P+ C +C CNQ + C + G CLNC+ N+ G NC C+ G+ DTS + CK C C + SL + N ++ C+CK G +G C++C + T PC I S + + +C KC N +K
Sbjct: 290 RCKCN--GHASRCIFDKMGRYTCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKSC-------NQSSGQCVCKPGVTGTTCNRCAKGY----QQSRSTVTPCIKIPTKADFIGSSHSEEQDQCSKCRIVPKRLNQKK 492
BLAST of Netrin-4 vs. Ensembl Celegans
Match: unc-6 (Netrin unc-6 [Source:UniProtKB/Swiss-Prot;Acc:P34710]) HSP 1 Score: 207.223 bits (526), Expect = 5.043e-56 Identity = 133/438 (30.37%), Postives = 211/438 (48.17%), Query Frame = 3
Query: 123 DNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGR-KSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGV--HNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERK--RSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRL--------------HTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGF 1379
D+ R C P + G+ AS TCG ++YC V G + +C CD++N +Q ++ + ++ + W S + NVS+ +S +F+ T+ + F + P +M + +S D+G+TW + ++S C R F P+ +SN E +CT+ S I+ G V F + + + D T+I+V F RL +++G+ + DD ++ +Y+Y++ E + GRC C GHAS+C +M CDC+HNT G CE CK F+ D PW A NS N C C CN H+ C FD +++ S SGGVC NC+HNT +NC CK G+ R+ ++ + + C C C +G+ S C Q ++G C+CK V G C++C G+
Sbjct: 39 DHTGRPVRCVPEFINAAFGKPVI--ASDTCGTNRPDKYCTVKEGPDGIIREQCDTCDARNHFQSHPASLLTDLNSIGN------MTCWVSTPSLSPQNVSLTLSLGKKFELTYVSMHFCSRLPDSMALYKSADFGKTWTPFQFYSSECRRIFGRDPDVSITKSNEQEAVCTA--SHIMGPGGNRVAFPFLENRPSAQNFENSPVLQDWVTATDIKVVFSRLSPDQAELYGLSNDVNSYGN-ETDDE--VKQRYFYSMGELAVGGRCKCNGHASRCI-------FDKMGRYTCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKS-CNQ--------SSGQCVCKPGVTGTTCNRCAKGY 447 HSP 2 Score: 75.8702 bits (185), Expect = 1.836e-13 Identity = 57/172 (33.14%), Postives = 80/172 (46.51%), Query Frame = 3
Query: 1047 KCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNI--AVAINSSQTCLKCHCDSLGTKFPSICIQEPEL---DLGLNAGHCI-CKENVEGNQCDKCKIGFWK---FNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGY 1535
+C+CN H+S C FD KM CD C+HNT CE CK +Y NS+ +C+ C+C+ + C + EL + G C+ C+ N G C CK GF + C C C +G+L + C+ S+G+C CK VTG C+ C GY
Sbjct: 290 RCKCNGHASRCIFD--------KMGRYTCD-CKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKR----CRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSL-GKSCNQSSGQCVCKPGVTGTTCNRCAKGY 447 HSP 3 Score: 62.3882 bits (150), Expect = 2.667e-9 Identity = 50/179 (27.93%), Postives = 75/179 (41.90%), Query Frame = 3
Query: 2769 CRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVR-------TCTKCVCNHMGTNSKWQGIC-----NHETGKCP-CLPNVVGLQCDRCQDKF-----FNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLD 3251
C+C+G+ + I D T +C+ NT+G CE C ++ R +C C CN ++ N G C C N G C C+ F ++ + C C C +P+G+L SCNQ +GQC C+ G +G CN C G+ + + C K D
Sbjct: 291 CKCNGHASRCIFDKMGRYTCDCKH---NTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGC-HPVGSLGKSCNQSSGQCVCKPGVTGTTCNRCAKGYQQSRSTVTPCIKIPTKAD 465 HSP 4 Score: 62.3882 bits (150), Expect = 2.736e-9 Identity = 48/171 (28.07%), Postives = 77/171 (45.03%), Query Frame = 3
Query: 2889 YFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFN-------LSSQIGCLPCNCD-------YPIGALNHSCNQLTGQCY-CQAGRSGKMCNNCKDGFWGDP----TKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKG 3344
++ G + +C CN + + + + C C N G +C+ C+ ++ +S C+ CNC+ + S N+ G C C+ +G+ C+ CK GF D T C C C+ GS+ K CN+++G C CKPG+TG C+ C KG
Sbjct: 279 FYSMGELAVGGRCKCNGHASRCIFDKMGRY---TCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKSCNQSSGQCVCKPGVTGTTCNRCAKG 446 HSP 5 Score: 62.003 bits (149), Expect = 3.659e-9 Identity = 57/216 (26.39%), Postives = 90/216 (41.67%), Query Frame = 3
Query: 2310 KCKCDLTGSLNSQCYNDTGK--CLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTC-----------DSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEK 2876
+CKC+ G + ++ G+ C C+ G C+ CKP +++ P+ C +C CNQ + C + G CLNC+ N+ G NC C+ G+ DTS + CK C C + SL + N ++ C+CK G +G C++C + T PC I S + + +C KC N +K
Sbjct: 290 RCKCN--GHASRCIFDKMGRYTCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKSC-------NQSSGQCVCKPGVTGTTCNRCAKGY----QQSRSTVTPCIKIPTKADFIGSSHSEEQDQCSKCRIVPKRLNQKK 492
BLAST of Netrin-4 vs. Ensembl Celegans
Match: lam-3 (LAMinin related. See also lmb; Laminin alpha chain [Source:UniProtKB/TrEMBL;Acc:G5ECE3]) HSP 1 Score: 211.46 bits (537), Expect = 1.958e-54 Identity = 159/513 (30.99%), Postives = 231/513 (45.03%), Query Frame = 3
Query: 174 IGRESFLRASSTCGLTNSERYCVVTTSDSGRKS------KCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHN-----VSIEISFESEFQFTFTHLRF-KTYRPAAMYIERSKD--YGRTWNKYAYFSDNCPRDF--PS---IPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNK------------YYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMS-------GGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGS 1598
+ S + A+ TCG +E YC + RK+ +C ICD+ N ++R H IE + RWWQS S + V+I I E+Q + L+ + RP +E+S D Y W YA C R F P+ +P ++ + EV CTSE+S I P E G + S VN ++ T R +FVRL ++ + I NK Y+Y+IS+ I G+C CYGHA C + Q K C+C+HNT G+ C +C +N PW P + N C++C+C +H+ SC +D + + ++ GG C C HNTE NCERCK GYYR ++ C C CD +G+ + + + G G CICK G +C++C G+ C C C G++ CD ++ C CK+ V G C CK G +LS +N GC C C + S
Sbjct: 55 LATNSLITATDTCGQYTAEEYCKLVEHVLLRKTTNTQSPQCDICDANNVHKR------HPIEYAID----GTRRWWQSPSLANGLRFEKVNITIDLRQEYQVAYIILKMGNSPRPGTWVLEKSLDGEYYEPWQYYAMQDAECMRQFGIPATTGVPRFQKED--EVHCTSEYSKITPLENGEIHTS-----LVNGRPGAENTSLELQKFT--RARFVRLRLISPRTLNADLMIINKKSDSLDKSVTMRYFYSISDISIGGQCICYGHAESCPSDPVTGQFK------CECRHNTCGESCNRCCPLFNQLPWKPGTNSHPNVCQQCQCFNHAHSCVYDDELDRNKWSITPEGVYEGGGRCLECAHNTEGFNCERCKDGYYRPSGLSHYREDACRTCECDPVGSVSDACVRDDQSAENGQKPGDCICKPGFGGRRCERCAPGY-----RNHPTCEPCPCNRAGSVNFDTCDGAS--CQCKANVEGIYCDRCKAGTIHLSASNPLGCQPCFCFGHSS 535 HSP 2 Score: 185.652 bits (470), Expect = 1.446e-46 Identity = 116/343 (33.82%), Postives = 181/343 (52.77%), Query Frame = 3
Query: 2370 CLCREGVEGQHCDTCKPGYWNF------PFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI---LCKKCQCPGLSSLKNN-------SIGCSLIFNTTAFTCM-CKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFK-QCNRNNGVCRCKPGITGRNCDMCMKG 3344
C C E G C++C PGY C C C+ S CD TG+CLNC+ N+ G C+ C+ G+ G+ SRG C++C+CP L N ++ S+ N + C+ C GY G C+ C F + ++ G C C C+GNID +C+S+TG+C KC+ +T+G +CE C ++GN + TC C C+ G + C+ E G+C C N +G QCDRC++ ++ + GC C+C+ G++ C+Q++GQC C+ G GK C+ C+ ++ GC C+CN+ GS+ +C++ G C C+ + G C+ C G
Sbjct: 741 CECPENFAGNSCESCVPGYRRVNNQLYGGRCEKCDCHGNSEECDPFTGECLNCRHNTTGSRCELCKPGHVGNPSRGGELGACEQCECPSLDLNPNPECISTELAVLGSVASNEDNYVCINCPLGYEGNKCEYCSDGFFEDPLT--GKCIECTCNGNIDPMGIGNCDSETGKCLKCIGHTTGDSCESCKEHHWGNAQLHTCKPCGCHTQGAVNPQ---CSEENGECECKENYIGAQCDRCKENHGDVEN--GCPACDCN-DTGSIGSDCDQVSGQCNCKQGVFGKQCDQCRPSYFNFTDA--GCQFCHCNIYGSIEDGKCDQTTGKCECRENVEGTMCEKCADG 1073 HSP 3 Score: 181.415 bits (459), Expect = 3.197e-45 Identity = 129/371 (34.77%), Postives = 185/371 (49.87%), Query Frame = 3
Query: 2169 CKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNF--PFCHSCKCNQMSTT----CDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDY----PIGALNHS--CNQLTGQCYCQAGRSGKMCNNCKDG-FWGDPTKLNGCTKCYC 3242
C C+ G+ N C++ G+C+C N G C+RC E++G GC C C+ TGS+ S C +G+C C++GV G+ CD C+P Y+NF C C CN + CD TGKC C+ N G C+ C +GY+ TS G C+ C C S + C+L+ C+CK G +G CD C N +G C+PC G + I+ SC C NT G CE C + + + C C C+ +G++ G+CN TG+C C VGL+CD C FFN + C PC C+ P+ + CN++ G+C C+ G C+ C +G F D + L GCT+C+C
Sbjct: 941 CGCHTQGAVNPQCSEENGECECKENYIGAQCDRC-KENHGDVENGCPACDCNDTGSIGSDCDQVSGQCNCKQGVFGKQCDQCRPSYFNFTDAGCQFCHCNIYGSIEDGKCDQTTGKC-ECRENVEGTMCEKCADGYFNITS-GDGCEDCGCDPTGS---EDVSCNLVTG----QCVCKPGVTGLKCDSCLPNFYGLTSEGCTECEPCPAPGQVCDPIDGSC--------VCPPNTVGEMCENCTTNAWDYHPLNGCKLCDCSDIGSDG---GMCNTFTGQCKCKAAYVGLKCDLCTHGFFNFPT---CEPCGCNAAGTDPLQCKDGQCLCNEI-GECPCKKNVHGTKCDQCGEGTFSLDSSNLKGCTECFC 1286 HSP 4 Score: 167.933 bits (424), Expect = 3.972e-41 Identity = 149/476 (31.30%), Postives = 199/476 (41.81%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQC-QCLPNVSGKTCERCASESYGFDARG-----CKKCKC-DLTGSLNSQCYNDTGKCL--------------CREGVEGQHCDTCKPGYWNFPF---CHSCKCN-----QMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSR--------------------------------GILCKKCQ---------CPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDS-CNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCG 3380
KC C+ + C+ G+C C N +G CE C G +RG C++C+C L + N +C + L C G EG C+ C G++ P C C CN CDS+TGKCL C G++ G +C++C+ ++G+ G C +C+ CP SIG + + C CK+G G+ CDQC ++F D CQ C C NI IED C+ TG+C +C N G CEKC GYF + C C C+ G+ CN TG+C C P V GL+CD C F+ L+S+ GC C P A C+ + G C C G+MC NC W D LNGC C C+ GS CN G C+CK G CD+C G P C CG
Sbjct: 773 KCDCH---GNSEECDPFTGECLNCRHNTTGSRCELCKPGHVGNPSRGGELGACEQCECPSLDLNPNPECISTELAVLGSVASNEDNYVCINCPLGYEGNKCEYCSDGFFEDPLTGKCIECTCNGNIDPMGIGNCDSETGKCLKCIGHTTGDSCESCKEHHWGNAQLHTCKPCGCHTQGAVNPQCSEENGECECKENYIGAQCDRCKENHGDVENGCPACDCNDTGSIGSDC--DQVSGQCNCKQGVFGKQCDQCRPSYFNFT---DAGCQFCHC--NIYGSIEDGKCDQTTGKC-ECRENVEGTMCEKCADGYFNITSGDGCEDCGCDPTGSEDV---SCNLVTGQCVCKPGVTGLKCDSCLPNFYGLTSE-GCTECE---PCPAPGQVCDPIDGSCVCPPNTVGEMCENCTTNAW-DYHPLNGCKLCDCSDIGSDGGMCNTFTGQCKCKAAYVGLKCDLCTHGF-FNFPTCEPCG 1228 HSP 5 Score: 163.696 bits (413), Expect = 6.802e-40 Identity = 132/417 (31.65%), Postives = 189/417 (45.32%), Query Frame = 3
Query: 2157 DPIKCKC-------NVTGSRNNNCNKIGGQC-QCLPNVSGKTCERCASESYG-FDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP-FCHSCKCNQ---MSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKC-VCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNN------GVCRCKPGITGRNCDMCMKGT 3347
DP+ KC N+ NC+ G+C +C+ + +G +CE C +G CK C C G++N QC + G+C C+E G CD CK + + C +C CN + + CD +G+C NCK FG C C Y+ T G C+ C C S+++ + T C C++ G C++C +F N S DG C+ C C + + SCN TG+C C +G C+ C+ ++G + CT+C C G +C+ G C C PN VG C+ C ++ GC C+C IG+ CN TGQC C+A G C+ C GF+ PT C C CN G+ QC G C CK + G CD C +GT
Sbjct: 880 DPLTGKCIECTCNGNIDPMGIGNCDSETGKCLKCIGHTTGDSCESCKEHHWGNAQLHTCKPCGCHTQGAVNPQCSEENGECECKENYIGAQCDRCKENHGDVENGCPACDCNDTGSIGSDCDQVSGQC-NCKQGVFGKQCDQCRPSYFNFTDAG--CQFCHCNIYGSIEDGKC------DQTTGKCECRENVEGTMCEKCADGYF-NITSGDG-CEDCGCDPTGSEDV--SCNLVTGQC-VCKPGVTGLKCDSCLPNFYGLTS-EGCTECEPCPAPGQ------VCDPIDGSCVCPPNTVGEMCENCTTNAWDYHPLNGCKLCDCS-DIGSDGGMCNTFTGQCKCKAAYVGLKCDLCTHGFFNFPT----CEPCGCNAAGTDPLQCKDGQCLCNEIGECPCKKNVHGTKCDQCGEGT 1270 HSP 6 Score: 102.834 bits (255), Expect = 2.055e-21 Identity = 81/268 (30.22%), Postives = 128/268 (47.76%), Query Frame = 3
Query: 915 VKEMVYGVCD--------CQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
+ M G CD C +T G CE CK+ + W A ++ C+ C C++ + +P +++ G C+ C+ N C+RCK + + C C C+ G+ S C Q +G C CK+ V G QCD+C+ ++ F + GC C+C + G++E+ CD +TGKC C+ V G C C GYFN+++ +GC C C GS D +C+ +G+C+CK G G++CD +Y
Sbjct: 895 IDPMGIGNCDSETGKCLKCIGHTTGDSCESCKEHH----WGNAQLHT---CKPCGCHTQGA---VNPQCSEEN-----GECE-CKENYIGAQCDRCKENHG-------DVENGCPACDCNDTGS-IGSDCDQV--------SGQCNCKQGVFGKQCDQCRPSYFNFT---DAGCQFCHCNIYGSIEDGKCDQTTGKCECRENVEGTMCEKCADGYFNITSG--DGCEDCGCDPTGSED-VSCNLVTGQCVCKPGVTGLKCDSCLPNFY 1124 HSP 7 Score: 98.5969 bits (244), Expect = 4.002e-20 Identity = 90/317 (28.39%), Postives = 137/317 (43.22%), Query Frame = 3
Query: 858 RCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFD------PVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRN-----------------IAVAINSSQT--CLKC-------HCDS----------LGTKFPSICIQEPELDL--GLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE 1676
+C C+G++ +C G+ +C+HNT G CE CK + + P+ C +CEC S + + + V+ + VC NC E CE C G++ + + + S+T CLKC C+S L T P C + ++ G C CKEN G QCD+CK + +G+ E GC C+C G++ CD +G+C CK V G C C+ YFN ++A GC C C+ YGS ++ CD+ +GKC C+ G C++
Sbjct: 773 KCDCHGNSEECDPFTGEC---------LNCRHNTTGSRCELCKPGHVGN---PSRGGELGACEQCECPSLDLNPNPECISTELAVLGSVASNEDNYVCINCPLGYEGNKCEYCSDGFFEDPLTGKCIECTCNGNIDPMGIGNCDSETGKCLKCIGHTTGDSCESCKEHHWGNAQLHTCKPCGCHTQGAVNPQCSEENGECECKENYIGAQCDRCK----ENHGDVENGCPACDCNDTGSI-GSDCDQVSGQCNCKQGVFGKQCDQCRPSYFNFTDA---GCQFCHCNIYGSIEDGKCDQTTGKCECRENVEGTMCEK 1069 HSP 8 Score: 94.3597 bits (233), Expect = 7.105e-19 Identity = 87/265 (32.83%), Postives = 120/265 (45.28%), Query Frame = 3
Query: 936 VCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNE-----CRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGH-----CI-CKENVEGNQCDKCKIGFWKFNGNEETG-CLKCNCVMMGTLENRG---CDSSTGKCY-CKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIK 1682
VC+C N G CE C +P + N+ C KC+C+ +S C DP G C NC+HNT CE CK G+ N + C +C C SL CI LG A + CI C EGN+C+ C GF++ + TG C++C C G ++ G CDS TGKC C TG+ C +CK ++ NA ++ C C C G+ N C + +G+C CK + G +CD K
Sbjct: 740 VCECPENFAGNSCESC---------VPGYRRVNNQLYGGRCEKCDCHGNSEEC--DPFT---------GECLNCRHNTTGSRCELCKPGHVGNPSRG-GELGACEQCECPSLDLNPNPECISTELAVLGSVASNEDNYVCINCPLGYEGNKCEYCSDGFFE---DPLTGKCIECTC--NGNIDPMGIGNCDSETGKCLKCIGHTTGDSCESCKEHHW--GNAQLHTCKPCGCHTQGAV-NPQCSEENGECECKENYIGAQCDRCK 975 HSP 9 Score: 87.0409 bits (214), Expect = 1.507e-16 Identity = 65/207 (31.40%), Postives = 97/207 (46.86%), Query Frame = 3
Query: 2367 KCL-CREGVEGQHCDTCKPGYWN---------------FPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI--LCKKCQCPGLSSLKNNSIGCSLIFNTTAFTC-MCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGY-FGNGAVRTCTKC 2927
KCL C +G G C + GY+ + C C+ S TC+ T C +C+ N+FG C++C GY GD G C KC CP + + ++S C + + + C CK GY+GQ+C+ C ++G+ GTC PC C + D + +CN +G+C +C +G C C + F N R CT C
Sbjct: 1496 KCLGCPQGYTGLSCQNPEVGYYRKKHREYLNQADDIALIGWSEPCSCHGHSQTCNPDTSVCTDCEHNTFGDFCEHCLPGYIGDAREGGANACTKCACPLVENSFSDS--CVAVDHGRGYVCNSCKPGYTGQYCETCVAGYYGDPQHIGGTCSPCDC--HPDGSLHGACNPLSGQC-ECKPGVTGRTCSMCQERHAFIN---RVCTSC 1694 HSP 10 Score: 75.8702 bits (185), Expect = 3.417e-13 Identity = 52/152 (34.21%), Postives = 69/152 (45.39%), Query Frame = 3
Query: 2217 GGQC-QCLPNVSGKTCERCASESY------GFDARGCKKCKCDLTGSLNSQCYND---------TGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQM-STTCDSKTGKCLNCKGNSFGYNCQNCENG-YYGDTSRGILCKKCQCPGLSS 2618
GG+C +C N G CERC Y + C+ C+CD GS++ C D G C+C+ G G+ C+ C PGY N P C C CN+ S D+ G CK N G C C+ G + S + C+ C C G SS
Sbjct: 384 GGRCLECAHNTEGFNCERCKDGYYRPSGLSHYREDACRTCECDPVGSVSDACVRDDQSAENGQKPGDCICKPGFGGRRCERCAPGYRNHPTCEPCPCNRAGSVNFDTCDGASCQCKANVEGIYCDRCKAGTIHLSASNPLGCQPCFCFGHSS 535 HSP 11 Score: 74.7146 bits (182), Expect = 7.593e-13 Identity = 47/134 (35.07%), Postives = 68/134 (50.75%), Query Frame = 3
Query: 2970 CNHETGKCP-CLPNVVGLQCDRCQDKFFNLSSQIG---CLPCNCDYPIGALNHSCNQLT-GQCY----CQAGRSGKMCNNCKDGFWGDPTKLNG-CTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMK 3341
CN +T C C N G C+ C + + + G C C C + + SC + G+ Y C+ G +G+ C C G++GDP + G C+ C C+ DGS+ CN +G C CKPG+TGR C MC +
Sbjct: 1549 CNPDTSVCTDCEHNTFGDFCEHCLPGYIGDAREGGANACTKCACPLVENSFSDSCVAVDHGRGYVCNSCKPGYTGQYCETCVAGYYGDPQHIGGTCSPCDCHPDGSLHGACNPLSGQCECKPGVTGRTCSMCQE 1682 HSP 12 Score: 71.2478 bits (173), Expect = 7.663e-12 Identity = 72/296 (24.32%), Postives = 112/296 (37.84%), Query Frame = 3
Query: 921 EMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNE-CRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDN------CQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTK------FPSICIQEPELD-----------LGLNA--------------------GHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE 1676
+ V G C+C+ G+ C++C+ P+ N T+ C+ C CN + S + G CD C+ N E CE+C GY+ I S C C CD G++ C+ +P + GL + G C+C N G C+ C W ++ GC C+C +G+ + C++ TG+C CK+ G C C G+FN GC+ +D + G+C CK G +CD+
Sbjct: 1001 DQVSGQCNCKQGVFGKQCDQCR---------PSYFNFTDAGCQFCHCNIYGS--------------IEDGKCDQTTGKCECRENVEGTMCEKCADGYFN-----ITSGDGCEDCGCDPTGSEDVSCNLVTGQCVCKPGVTGLKCDSCLPNFYGLTSEGCTECEPCPAPGQVCDPIDGSCVCPPNTVGEMCENCTTNAWDYH--PLNGCKLCDCSDIGS-DGGMCNTFTGQCKCKAAYVGLKCDLCTHGFFNFPTCEPCGCNAAGTDPLQCKDGQCLCNEIGECPCKKNVHGTKCDQ 1265 HSP 13 Score: 69.707 bits (169), Expect = 2.424e-11 Identity = 63/211 (29.86%), Postives = 86/211 (40.76%), Query Frame = 3
Query: 2466 MSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL-----------CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDG-TCQPCRCSGNIDQRIEDSC----NSKTGECRKCLFNTSGFNCEKCVSGYFGNGAV--RTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDK 3044
+STT KCL C G +CQN E GYY R L + C C G S N T+ C+ G FC+ C + G+A C C C ++ DSC + + C C +G CE CV+GY+G+ TC+ C C+ G+ G CN +G+C C P V G C CQ++
Sbjct: 1486 LSTTKAIGVEKCLGCPQGYTGLSCQNPEVGYYRKKHREYLNQADDIALIGWSEPCSCHGHSQTCNPD---------TSVCTDCEHNTFGDFCEHCLPGYIGDAREGGANACTKCACP-LVENSFSDSCVAVDHGRGYVCNSCKPGYTGQYCETCVAGYYGDPQHIGGTCSPCDCHPDGS---LHGACNPLSGQCECKPGVTGRTCSMCQER 1683 HSP 14 Score: 65.4698 bits (158), Expect = 4.913e-10 Identity = 46/137 (33.58%), Postives = 63/137 (45.99%), Query Frame = 3
Query: 2985 GKC-PCLPNVVGLQCDRCQDKFFNLSS-----QIGCLPCNCDYPIGALNHSC---------NQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSV-FKQCNRNNGVCRCKPGITGRNCDMCMKGT 3347
G+C C N G C+RC+D ++ S + C C CD P+G+++ +C Q G C C+ G G+ C C G+ PT C C CN GSV F C+ C+CK + G CD C GT
Sbjct: 385 GRCLECAHNTEGFNCERCKDGYYRPSGLSHYREDACRTCECD-PVGSVSDACVRDDQSAENGQKPGDCICKPGFGGRRCERCAPGYRNHPT----CEPCPCNRAGSVNFDTCD--GASCQCKANVEGIYCDRCKAGT 514 HSP 15 Score: 64.3142 bits (155), Expect = 1.101e-9 Identity = 70/237 (29.54%), Postives = 98/237 (41.35%), Query Frame = 3
Query: 1047 KCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQ--TCLKCHCDSLGTKFPSICIQEPELD----------LGLNAGHCI-CKENVEGNQCDKCKIGFWKFNG---NEETGCLKCNCVMMGTLEN---RGCDSS-----TGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKS 1685
+C C H+ SC DPV Q C+ C+HNT ++C RC + + +S C +C C C+ + ELD + G C+ C N EG C++CK G+++ +G E C C C +G++ + R S+ G C CK G C C GY N C C C+ GS + TCD S C CK GI CD K+
Sbjct: 295 QCICYGHAESCPSDPVTGQFK-------CE-CRHNTCGESCNRCCPLFNQLPWKPGTNSHPNVCQQCQC----FNHAHSCVYDDELDRNKWSITPEGVYEGGGRCLECAHNTEGFNCERCKDGYYRPSGLSHYREDACRTCECDPVGSVSDACVRDDQSAENGQKPGDCICKPGFGGRRCERCAPGYRNHPT-----CEPCPCNRAGSVNFDTCDGAS--CQCKANVEGIYCDRCKA 512 HSP 16 Score: 62.3882 bits (150), Expect = 4.154e-9 Identity = 54/199 (27.14%), Postives = 81/199 (40.70%), Query Frame = 3
Query: 930 YGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCK 1526
Y CQ+ G + +K +++ N + D C C+ HS +C+ D VC +C+HNT CE C GY + + C KC C + F C+ +D G CK G C+ C G++ + C C+C G+L C+ +G+C CK VTG CS C+
Sbjct: 1504 YTGLSCQNPEVGYYRKKHREYLNQA----DDIALIGWSEPCSCHGHSQTCNPDT-----------SVCTDCEHNTFGDFCEHCLPGYIGDAREG--GANACTKCACPLVENSFSDSCVA---VDHGRGYVCNSCKPGYTGQYCETCVAGYYGDPQHIGGTCSPCDCHPDGSLHG-ACNPLSGQCECKPGVTGRTCSMCQ 1681 HSP 17 Score: 60.077 bits (144), Expect = 1.763e-8 Identity = 64/226 (28.32%), Postives = 93/226 (41.15%), Query Frame = 3
Query: 2658 TTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGT------CQPCRCSGNI-----DQRIEDSCNSKT--------GECRKCLFNTSGFNCEKCVSGYFGNGAVR-----TCTKCVCNHMGTNSKW-----QGICN-HETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDG-FWGDPTKLNGCTKCYC 3242
T F C C+ G+ C++C F K GT CQ C+C + D ++ + S T G C +C NT GFNCE+C GY+ + C C C+ +G+ S Q N + G C C P G +C+RC + N + C PC C+ +C+ + C C+A G C+ CK G + GC C+C
Sbjct: 311 TGQFKCECRHNTCGESCNRC-CPLFNQLPWKPGTNSHPNVCQQCQCFNHAHSCVYDDELDRNKWSITPEGVYEGGGRCLECAHNTEGFNCERCKDGYYRPSGLSHYREDACRTCECDPVGSVSDACVRDDQSAENGQKPGDCICKPGFGGRRCERCAPGYRNHPT---CEPCPCNRAGSVNFDTCDGAS--CQCKANVEGIYCDRCKAGTIHLSASNPLGCQPCFC 530 HSP 18 Score: 58.151 bits (139), Expect = 8.345e-8 Identity = 66/253 (26.09%), Postives = 92/253 (36.36%), Query Frame = 3
Query: 2838 KCLFNTSGFNCEKCVSGY-------FG---------------------------NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCL---PN-----------------------------VVGLQCDRCQDKFFNLSSQIGCLPCNCDY---PIGALNHSCNQLTGQCY-CQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDC 3386
+C N +G +CE CV GY +G N C C H+G S+ + E +CP L PN G +C+ C D FF C+ C C+ P+G N C+ TG+C C +G C +CK+ WG+ +L+ C C C+ G+V QC+ NG C CK G CD C + C +C DC
Sbjct: 742 ECPENFAGNSCESCVPGYRRVNNQLYGGRCEKCDCHGNSEECDPFTGECLNCRHNTTGSRCELCKPGHVGNPSRGGELGACEQCECPSLDLNPNPECISTELAVLGSVASNEDNYVCINCPLGYEGNKCEYCSDGFFEDPLTGKCIECTCNGNIDPMGIGN--CDSETGKCLKCIGHTTGDSCESCKEHHWGN-AQLHTCKPCGCHTQGAVNPQCSEENGECECKENYIGAQCDRCKENHGDVENGCPAC-DC 990 HSP 19 Score: 52.7582 bits (125), Expect = 2.943e-6 Identity = 67/257 (26.07%), Postives = 94/257 (36.58%), Query Frame = 3
Query: 933 GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECN---SHSSSCHFD-------------------PVVYQQSRK---------MSGGVCDN------CQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGL---NAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKC 1583
G C+C+ N +G CEKC D Y + S + C C C+ S SC+ P Y + + G VCD C NT + CE C + A + C C C +G+ D G+ G C CK G +CD C GF+ F E GC + + + + G+C CK V G C C G F+L ++N+ GC+ C C
Sbjct: 1054 GKCECRENVEGTMCEKCADGY-------FNITSGDGCEDCGCDPTGSEDVSCNLVTGQCVCKPGVTGLKCDSCLPNFYGLTSEGCTECEPCPAPGQVCDPIDGSCVCPPNTVGEMCENCTTN-----AWDYHPLNGCKLCDCSDIGS------------DGGMCNTFTGQCKCKAAYVGLKCDLCTHGFFNFPTCEPCGCNAAGTDPLQCKDGQCLCNEIGECPCKKNVHGTKCDQCGEGTFSLDSSNLKGCTECFC 1286 HSP 20 Score: 52.7582 bits (125), Expect = 3.395e-6 Identity = 56/177 (31.64%), Postives = 70/177 (39.55%), Query Frame = 3
Query: 861 CSCYGHASKCKAKEGQVQVKEMVYGVC-DCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCEC----NSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLN--AGHCICKENVEGNQCDKCK 1370
CSC+GH+ C VC DC+HNT G CE C Y A + N C KC C NS S SC V R G VC++C+ + CE C +GYY + + TC C C G+ L N +G C CK V G C C+
Sbjct: 1540 CSCHGHSQTCNPDT----------SVCTDCEHNTFGDFCEHCLPGYIGD----AREGGANACTKCACPLVENSFSDSC----VAVDHGR---GYVCNSCKPGYTGQYCETCVAGYYGD---PQHIGGTCSPCDCHPDGS-----------LHGACNPLSGQCECKPGVTGRTCSMCQ 1681 HSP 21 Score: 52.7582 bits (125), Expect = 3.423e-6 Identity = 48/167 (28.74%), Postives = 70/167 (41.92%), Query Frame = 3
Query: 2352 YNDTGKCL-CREGVEGQHCDTCKPGYWNFP--------FCHSCKCNQMSTTCDS------------KTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNT-TAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGN 2786
Y G+CL C EG +C+ CK GY+ C +C+C+ + + D+ K G C+ CK G C+ C GY + C+ C C N G S+ F+T +C CK G +CD+C+ + S CQPC C G+
Sbjct: 381 YEGGGRCLECAHNTEGFNCERCKDGYYRPSGLSHYREDACRTCECDPVGSVSDACVRDDQSAENGQKPGDCI-CKPGFGGRRCERCAPGYRNHPT----CEPCPC--------NRAG-SVNFDTCDGASCQCKANVEGIYCDRCKAGTIHLSASNPLGCQPCFCFGH 533
BLAST of Netrin-4 vs. Ensembl Fly
Match: LanB1 (gene:FBgn0261800 transcript:FBtr0079490) HSP 1 Score: 437.573 bits (1124), Expect = 4.585e-126 Identity = 230/546 (42.12%), Postives = 317/546 (58.06%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSK-----NPYQRFSSTHSHRIENVV--SRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE----MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEI 1736
C ++ C P G+LLIGRE+ L ASSTCGL + ER+C+++ + KC +CD++ +PY+ +HRI ++ ++ + WWQS +G N +I++ E+EF FT + F T+RPAAMYIERS D+G+TW+ Y YF+ +C FP +P NI +V+CTS +S++ PS G V+F + D Y +N + +TN+R+Q +LH GD +D KYYY IS ++RG CSCYGHAS+C + + MV+G C+C HNTKG +CE+C+DF+ND PW PA TN C+KCECN H+ SCHFD V+ S +SGGVCDNC HNT ++CE C +YR+ I S + C C CD G+ IC EL+ G AG C CK V G +C++CK G+W + GC C C +GTL N GC TG+C CK +VTG DC+ C + LS + GCSLC C GS DN CD SG+C C+ G C + K ++I P P H E+
Sbjct: 49 CERSSCYPATGNLLIGRENRLTASSTCGLHSPERFCILSHL---QDKKCFLCDTREETKHDPYK------NHRIGQIIYKTKPGTNIPTWWQSENGKENATIQLDLEAEFHFTHLIITFTTFRPAAMYIERSFDFGQTWHIYRYFAYDCKESFPGVP-TVLENITDVMCTSRYSNVEPSRNGEVIFRVLPPNINVTDPYAEHVQNQLK-MTNLRIQMTKLHKLGDNLLDSRLENEEKYYYGISNMVVRGSCSCYGHASQCLPLDPAFSQADNEDGMVHGRCECTHNTKGMNCEECEDFFNDLPWKPAFGKKTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSDDGICDSLNELEEGAVAGACHCKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCNPLGTLNNSGCVMRTGECKCKKYVTGKDCNQCMPETYGLSES-PEGCSLCNCDAGGSYDN-YCDVISGQCRCRPHMTGRSCSQPKQNYFI--PLLPEVHEAEV 579 HSP 2 Score: 376.326 bits (965), Expect = 6.218e-106 Identity = 256/830 (30.84%), Postives = 411/830 (49.52%), Query Frame = 3
Query: 2169 CKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRG--ILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFD---------KLHNSSGVFMSNV-KFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIID-FNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKS-LKNILNGASESKKIYQNITLIKDKLFYRNEELRNI--NLKFIRQKSKDMDNLINEMSKN--VITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKP--ESEYNTTIKLIDVTRGAIQSRR-IESVNLLIKIKTYL---------NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNI 4565
C CN TGS + C GG CQC PNV G+ C++CA +YGF GCK C C+ GS + C TG+C C G+ C+ C+PGYWNFP C C+CN + TCD G C++C+ ++ GY+C +C +GYYG+ G I C+ C+CP ++S ++ GCSL C C++GYSG C+ C N FGN GTC C CS N+D +C+ +TG C KCL+ T+G +CE C G+FG+ + C +C C+ +GTN+ C+ TG+CPCLPNV G++CD+C + + ++S GC CNCD PIGAL+ CN TGQC C+ G G+ CN C+ +WG+P + C C C+ G+ QC+R G C C GI G C+ C +G G+ P+CS CG+C+ WD L +++ K ++G + S + K++ + L+ SL +I+K + Y+ + S + N+ D +N+ S + +E+ ++++ + +K G +L++S ++ LN + + N++ +KD+ NE N N K + S + ++++ N +I ++T P + ++ CG G D C + G F + +T+ LK + K+ D ++ +++ N + RG QS R + N IK+ L N +A + + L + ++ + EI L QI + L NV I+ ++K + + + + A KE +L+S ++
Sbjct: 789 CNCNPTGSLSKVCESNGGYCQCKPNVVGRQCDQCAPGTYGFGPEGCKACDCNSIGSKDKYCDLITGQCQCVPNTYGRECNQCQPGYWNFPECRVCQCNGHAATCDPIQGTCIDCQDSTTGYSCDSCLDGYYGNPLFGSEIGCRPCRCPETVASGLAHADGCSLDTRNNNMLCHCQEGYSGSRCEICADNFFGNP-DNGGTCSKCECSNNVDLYDTGNCDRQTGACLKCLYQTTGDHCELCKDGFFGDALQQNCQQCECDFLGTNNTI-AHCDRFTGQCPCLPNVQGVRCDQCAENHWKIASGEGCESCNCD-PIGALHEQCNSYTGQCQCKPGFGGRACNQCQAHYWGNPNE--KCQPCECDQFGAADFQCDRETGNCVCHEGIGGYKCNECARGYIGQFPHCSPCGECFNNWDLILSALEDATTATILRAKEIKQVGATGAYTSEFSELDKKLQHIRNLLQNTSVSLVDIEK---LDYETQSLRDQLQASHGRLSETEQNLDDIYNSLSLSGVELESLQNHSRLVQQLSKELKENGI--QLQESNIEGALNLTRHAYERVSNLSTLKDEA---NELASNTDRNCKRVENLSNKIQAEADDLANNNKLIEDYRAELTSLTSQIPELNNQVCGKPG---DPC--DSLCGGAGCGHCGGFLSCEHGA---KTHSEEALKVAKDAETAITSKKDQADQTIRALTQAKLNASEAYEKAKRGFEQSERYLNQTNANIKLAENLFIALNNFQENKTASPSESKELAQKTLDLDLKLEPEEIETLGDQINRAVSSLKNVEAIIYRTKPDLDRVNNLQSIANATKEKADKILDSANSV 1597 HSP 3 Score: 107.071 bits (266), Expect = 1.105e-22 Identity = 79/248 (31.85%), Postives = 112/248 (45.16%), Query Frame = 3
Query: 936 VCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSR-KMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE 1676
+C CQ G CE C D + +P D T C KCEC+++ +Y G C C + T +CE CK G++ + Q C +C CD LGT G C C NV+G +CD+C WK E GC CNC +G L + C+S TG+C CK G C+ C+ Y+ N C C+C ++G+ D + CD+ +G C+C G G +C+E
Sbjct: 959 LCHCQEGYSGSRCEICADNFFGNP----DNGGT--CSKCECSNNVD-------LYDTGNCDRQTGACLKCLYQTTGDHCELCKDGFFGDAL-----QQNCQQCECDFLGTN-------NTIAHCDRFTGQCPCLPNVQGVRCDQCAENHWKIASGE--GCESCNCDPIGALHEQ-CNSYTGQCQCKPGFGGRACNQCQAHYWGNPNEK---CQPCECDQFGAADFQ-CDRETGNCVCHEGIGGYKCNE 1174 HSP 4 Score: 103.99 bits (258), Expect = 1.143e-21 Identity = 81/247 (32.79%), Postives = 119/247 (48.18%), Query Frame = 3
Query: 2673 CMCKKGYSGQFCDQCETNHFGNAIS-------KDGTCQPCRC-----SGNIDQRI-EDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNG-----AVRTCTKCVCNHMGTNSKWQGICNH--------ETGKCPCLPNVVGLQCDRCQDKFFNLSSQ--IGCLPCNCDYPIGALNHS-CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNC 3326
C C G C++CE F N + K C+ C C S + D+ + S G C CL NT G +CE+C+ ++ + + R C C C+ G++ GIC+ G C C V G +C++C+D ++NL S GC PC C+ P+G LN+S C TG+C C+ +GK CN C +G GC+ C C+ GS C+ +G CRC+P +TGR+C
Sbjct: 318 CECTHNTKGMNCEECED--FFNDLPWKPAFGKKTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSD--DGICDSLNELEEGAVAGACHCKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCN-PLGTLNNSGCVMRTGECKCKKYVTGKDCNQCMPETYGLSESPEGCSLCNCDAGGSYDNYCDVISGQCRCRPHMTGRSC 559 HSP 5 Score: 102.834 bits (255), Expect = 2.282e-21 Identity = 85/268 (31.72%), Postives = 123/268 (45.90%), Query Frame = 3
Query: 921 EMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCD---SLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETG--CLKCNCVMMGTLENRG-CDSSTGKCY-CKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDN-KTCDKYSGKCLCKNGFAGIRCDE-IKSEWYI 1697
+++ G C C NT G+ C +C+ Y + P ECR C+CN H+++C DP+ G C +CQ +T +C+ C GYY N S C C C + G C LD N C C+E G++C+ C F+ GN + G C KC C L + G CD TG C C TG+ C CK G+F +A C C+C G+ + CD+++G+C C G+RCD+ ++ W I
Sbjct: 850 DLITGQCQCVPNTYGRECNQCQPGYWNFP----------ECRVCQCNGHAATC--DPIQ---------GTCIDCQDSTTGYSCDSCLDGYYGN--PLFGSEIGCRPCRCPETVASGLAHADGC----SLDTRNNNMLCHCQEGYSGSRCEICADNFF---GNPDNGGTCSKCECSNNVDLYDTGNCDRQTGACLKCLYQTTGDHCELCKDGFF--GDALQQNCQQCECDFLGTNNTIAHCDRFTGQCPCLPNVQGVRCDQCAENHWKI 1085 HSP 6 Score: 84.7297 bits (208), Expect = 8.602e-16 Identity = 69/223 (30.94%), Postives = 100/223 (44.84%), Query Frame = 3
Query: 2826 GECRKCLFNTSGFNCEKCVSGY--------FGNGAVRTCTKCVCNHMGTNSKWQ------------GICNHETGKCPCLPNVVGLQCDRCQDKFF-----NLSSQIGCLPCNCDYPIGALNH----SCNQL-----TGQCYCQAGRSGKMCNNCKDGFWG-DPTKLNGCTKCYCNLDGSVFKQ-CNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDC 3386
G C +C NT G NCE+C + FG C KC CN + + G+C++ CL N G C+ C F+ +++S+ C PC+CD P G+ + S N+L G C+C+A +G+ CN CKDG+W GC C CN G++ C G C+CK +TG++C+ CM T G + C C
Sbjct: 316 GRC-ECTHNTKGMNCEECEDFFNDLPWKPAFGK-KTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDN------CLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCD-PQGSSDDGICDSLNELEEGAVAGACHCKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCNPLGTLNNSGCVMRTGECKCKKYVTGKDCNQCMPETYGLSESPEGCSLC 529 HSP 7 Score: 82.0333 bits (201), Expect = 4.487e-15 Identity = 76/264 (28.79%), Postives = 121/264 (45.83%), Query Frame = 3
Query: 2493 GKCLNCKGNSFGYNCQNCENGYYGDTS-------RGILCKKCQCPGLSSLKNNSIGCSL---IFNTTAFTCM-----CKKGYSGQFCDQCETNHFGNA---ISKDGTCQPCRC--SGNIDQRIEDSCN-----SKTGECRKCLFNTSGFNCEKCVSGYFG--NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLS-SQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFW 3200
G+C C N+ G NC+ CE+ ++ D + CKKC+C ++++ C +F + F C GQ C++C + + I+ + CQPC C G+ D I DS N + G C C +G C +C GY+ + C C CN +GT + C TG+C C V G C++C + + LS S GC CNCD G+ ++ C+ ++GQC C+ +G+ C+ K ++
Sbjct: 316 GRC-ECTHNTKGMNCEECED-FFNDLPWKPAFGKKTNACKKCEC------NDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSDDGICDSLNELEEGAVAGACH-CKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCNPLGTLNN--SGCVMRTGECKCKKYVTGKDCNQCMPETYGLSESPEGCSLCNCDAG-GSYDNYCDVISGQCRCRPHMTGRSCSQPKQNYF 567 HSP 8 Score: 76.2554 bits (186), Expect = 2.810e-13 Identity = 65/260 (25.00%), Postives = 116/260 (44.62%), Query Frame = 3
Query: 2364 GKCLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTCD-----------SKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKN---NSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCS--GNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFG-NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFF 3050
G+C C +G +C+ C+ + + P+ C C+CN + +C G C NC N+ G +C+ C +Y D + I +C+ C C S + +S+ L A C CK +G+ C+QC+ ++ C+PC C+ G ++ C +TGEC KC +G +C +C+ +G + + C+ C C+ G+ + C+ +G+C C P++ G C + + +F
Sbjct: 316 GRCECTHNTKGMNCEECEDFFNDLPWKPAFGKKTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSDDGICDSLN-ELEEGAVAGACHCKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCNPLGTLNN---SGCVMRTGEC-KCKKYVTGKDCNQCMPETYGLSESPEGCSLCNCDAGGS---YDNYCDVISGQCRCRPHMTGRSCSQPKQNYF 567 HSP 9 Score: 73.559 bits (179), Expect = 1.887e-12 Identity = 46/129 (35.66%), Postives = 69/129 (53.49%), Query Frame = 3
Query: 1311 NAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCL-CKNGFAGIRCDEIKSEWY 1694
N G+C CK NV G QCD+C G + F G E GC C+C +G+ +++ CD TG+C C G +C+ C+ GY+N C +C+C+ + + TCD G C+ C++ G CD +Y
Sbjct: 804 NGGYCQCKPNVVGRQCDQCAPGTYGF-GPE--GCKACDCNSIGS-KDKYCDLITGQCQCVPNTYGRECNQCQPGYWNFPE-----CRVCQCNGHAA----TCDPIQGTCIDCQDSTTGYSCDSCLDGYY 919 HSP 10 Score: 71.633 bits (174), Expect = 6.963e-12 Identity = 71/267 (26.59%), Postives = 112/267 (41.95%), Query Frame = 3
Query: 2214 IGGQCQCLPNVSGKTCERCAS--------ESYGFDARGCKKCKCD---LTGSLNSQCYNDTGKCL------CREGVEGQHCDTCKPGYWNFP--------FCHSCKCNQMSTT----CDS--------KTGKCLNCKGNSFGYNCQNCENGYY---GDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYF 2894
+ G+C+C N G CE C ++G CKKC+C+ ++ + + +G C GQHC+ C P ++ P C C C+ ++ CDS G C +CK G C C++GY+ D G C+ C C L +L N+ GC + C CKK +G+ C+QC +G + S +G C C C ++ C+ +G+CR C + +G +C + YF
Sbjct: 314 VHGRCECTHNTKGMNCEECEDFFNDLPWKPAFGKKTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSDDGICDSLNELEEGAVAGAC-HCKAFVTGRRCNQCKDGYWNLQSDNPEG--CEPCTCNPLGTLNNS--GCVM----RTGECKCKKYVTGKDCNQCMPETYGLSESPEG-CSLCNCDAG--GSYDNYCDVISGQCR-CRPHMTGRSCSQPKQNYF 567 HSP 11 Score: 66.6254 bits (161), Expect = 2.787e-10 Identity = 59/209 (28.23%), Postives = 87/209 (41.63%), Query Frame = 3
Query: 933 GVC-DCQHNTKGQHCEKCKD-FYNDSPWLPADKNSTNECRKCECN---SHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNA--GHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYF 1538
G C C + T G HCE CKD F+ D+ C++CEC+ ++++ H D G C C N + C++C +++ I S + C C+CD +G L N+ G C CK G C++C+ +W GN C C C G + + CD TG C C + G C+ C GY
Sbjct: 1010 GACLKCLYQTTGDHCELCKDGFFGDA--------LQQNCQQCECDFLGTNNTIAHCDRFT---------GQCP-CLPNVQGVRCDQCAENHWK-----IASGEGCESCNCDPIGA-----------LHEQCNSYTGQCQCKPGFGGRACNQCQAHYW---GNPNEKCQPCECDQFGAADFQ-CDRETGNCVCHEGIGGYKCNECARGYI 1180 HSP 12 Score: 59.3066 bits (142), Expect = 3.594e-8 Identity = 55/194 (28.35%), Postives = 79/194 (40.72%), Query Frame = 3
Query: 1122 GGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCY-CKSFVTGNDCSTCKIGYFN--LSNANINGCSLCKCSEYGSRDNKTCDKYSGK-------CLCKNGFAGIRCD 1673
GG C C+ N + C++C G Y + C C C+S+G+K + L G C C N G +C++C+ G+W F C C C CD G C C+ TG C +C GY+ L + I GC C+C E + D S C C+ G++G RC+
Sbjct: 805 GGYC-QCKPNVVGRQCDQCAPGTYG------FGPEGCKACDCNSIGSK---------DKYCDLITGQCQCVPNTYGRECNQCQPGYWNF-----PECRVCQCNGHAAT----CDPIQGTCIDCQDSTTGYSCDSCLDGYYGNPLFGSEI-GCRPCRCPETVASGLAHADGCSLDTRNNNMLCHCQEGYSGSRCE 972 HSP 13 Score: 58.5362 bits (140), Expect = 6.161e-8 Identity = 34/90 (37.78%), Postives = 48/90 (53.33%), Query Frame = 3
Query: 2169 CKCNVTGSRNNN-CNKIGGQCQCLPNVSGKTCERCASESYGFD--ARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYW 2429
C CN G+ NN+ C G+C+C V+GK C +C E+YG GC C CD GS ++ C +G+C CR + G+ C K Y+
Sbjct: 478 CTCNPLGTLNNSGCVMRTGECKCKKYVTGKDCNQCMPETYGLSESPEGCSLCNCDAGGSYDNYCDVISGQCRCRPHMTGRSCSQPKQNYF 567
BLAST of Netrin-4 vs. Ensembl Fly
Match: LanB1 (gene:FBgn0261800 transcript:FBtr0079491) HSP 1 Score: 437.573 bits (1124), Expect = 4.585e-126 Identity = 230/546 (42.12%), Postives = 317/546 (58.06%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSK-----NPYQRFSSTHSHRIENVV--SRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE----MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEI 1736
C ++ C P G+LLIGRE+ L ASSTCGL + ER+C+++ + KC +CD++ +PY+ +HRI ++ ++ + WWQS +G N +I++ E+EF FT + F T+RPAAMYIERS D+G+TW+ Y YF+ +C FP +P NI +V+CTS +S++ PS G V+F + D Y +N + +TN+R+Q +LH GD +D KYYY IS ++RG CSCYGHAS+C + + MV+G C+C HNTKG +CE+C+DF+ND PW PA TN C+KCECN H+ SCHFD V+ S +SGGVCDNC HNT ++CE C +YR+ I S + C C CD G+ IC EL+ G AG C CK V G +C++CK G+W + GC C C +GTL N GC TG+C CK +VTG DC+ C + LS + GCSLC C GS DN CD SG+C C+ G C + K ++I P P H E+
Sbjct: 49 CERSSCYPATGNLLIGRENRLTASSTCGLHSPERFCILSHL---QDKKCFLCDTREETKHDPYK------NHRIGQIIYKTKPGTNIPTWWQSENGKENATIQLDLEAEFHFTHLIITFTTFRPAAMYIERSFDFGQTWHIYRYFAYDCKESFPGVP-TVLENITDVMCTSRYSNVEPSRNGEVIFRVLPPNINVTDPYAEHVQNQLK-MTNLRIQMTKLHKLGDNLLDSRLENEEKYYYGISNMVVRGSCSCYGHASQCLPLDPAFSQADNEDGMVHGRCECTHNTKGMNCEECEDFFNDLPWKPAFGKKTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSDDGICDSLNELEEGAVAGACHCKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCNPLGTLNNSGCVMRTGECKCKKYVTGKDCNQCMPETYGLSES-PEGCSLCNCDAGGSYDN-YCDVISGQCRCRPHMTGRSCSQPKQNYFI--PLLPEVHEAEV 579 HSP 2 Score: 376.326 bits (965), Expect = 6.218e-106 Identity = 256/830 (30.84%), Postives = 411/830 (49.52%), Query Frame = 3
Query: 2169 CKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRG--ILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFD---------KLHNSSGVFMSNV-KFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIID-FNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKS-LKNILNGASESKKIYQNITLIKDKLFYRNEELRNI--NLKFIRQKSKDMDNLINEMSKN--VITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKP--ESEYNTTIKLIDVTRGAIQSRR-IESVNLLIKIKTYL---------NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNI 4565
C CN TGS + C GG CQC PNV G+ C++CA +YGF GCK C C+ GS + C TG+C C G+ C+ C+PGYWNFP C C+CN + TCD G C++C+ ++ GY+C +C +GYYG+ G I C+ C+CP ++S ++ GCSL C C++GYSG C+ C N FGN GTC C CS N+D +C+ +TG C KCL+ T+G +CE C G+FG+ + C +C C+ +GTN+ C+ TG+CPCLPNV G++CD+C + + ++S GC CNCD PIGAL+ CN TGQC C+ G G+ CN C+ +WG+P + C C C+ G+ QC+R G C C GI G C+ C +G G+ P+CS CG+C+ WD L +++ K ++G + S + K++ + L+ SL +I+K + Y+ + S + N+ D +N+ S + +E+ ++++ + +K G +L++S ++ LN + + N++ +KD+ NE N N K + S + ++++ N +I ++T P + ++ CG G D C + G F + +T+ LK + K+ D ++ +++ N + RG QS R + N IK+ L N +A + + L + ++ + EI L QI + L NV I+ ++K + + + + A KE +L+S ++
Sbjct: 789 CNCNPTGSLSKVCESNGGYCQCKPNVVGRQCDQCAPGTYGFGPEGCKACDCNSIGSKDKYCDLITGQCQCVPNTYGRECNQCQPGYWNFPECRVCQCNGHAATCDPIQGTCIDCQDSTTGYSCDSCLDGYYGNPLFGSEIGCRPCRCPETVASGLAHADGCSLDTRNNNMLCHCQEGYSGSRCEICADNFFGNP-DNGGTCSKCECSNNVDLYDTGNCDRQTGACLKCLYQTTGDHCELCKDGFFGDALQQNCQQCECDFLGTNNTI-AHCDRFTGQCPCLPNVQGVRCDQCAENHWKIASGEGCESCNCD-PIGALHEQCNSYTGQCQCKPGFGGRACNQCQAHYWGNPNE--KCQPCECDQFGAADFQCDRETGNCVCHEGIGGYKCNECARGYIGQFPHCSPCGECFNNWDLILSALEDATTATILRAKEIKQVGATGAYTSEFSELDKKLQHIRNLLQNTSVSLVDIEK---LDYETQSLRDQLQASHGRLSETEQNLDDIYNSLSLSGVELESLQNHSRLVQQLSKELKENGI--QLQESNIEGALNLTRHAYERVSNLSTLKDEA---NELASNTDRNCKRVENLSNKIQAEADDLANNNKLIEDYRAELTSLTSQIPELNNQVCGKPG---DPC--DSLCGGAGCGHCGGFLSCEHGA---KTHSEEALKVAKDAETAITSKKDQADQTIRALTQAKLNASEAYEKAKRGFEQSERYLNQTNANIKLAENLFIALNNFQENKTASPSESKELAQKTLDLDLKLEPEEIETLGDQINRAVSSLKNVEAIIYRTKPDLDRVNNLQSIANATKEKADKILDSANSV 1597 HSP 3 Score: 107.071 bits (266), Expect = 1.105e-22 Identity = 79/248 (31.85%), Postives = 112/248 (45.16%), Query Frame = 3
Query: 936 VCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSR-KMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE 1676
+C CQ G CE C D + +P D T C KCEC+++ +Y G C C + T +CE CK G++ + Q C +C CD LGT G C C NV+G +CD+C WK E GC CNC +G L + C+S TG+C CK G C+ C+ Y+ N C C+C ++G+ D + CD+ +G C+C G G +C+E
Sbjct: 959 LCHCQEGYSGSRCEICADNFFGNP----DNGGT--CSKCECSNNVD-------LYDTGNCDRQTGACLKCLYQTTGDHCELCKDGFFGDAL-----QQNCQQCECDFLGTN-------NTIAHCDRFTGQCPCLPNVQGVRCDQCAENHWKIASGE--GCESCNCDPIGALHEQ-CNSYTGQCQCKPGFGGRACNQCQAHYWGNPNEK---CQPCECDQFGAADFQ-CDRETGNCVCHEGIGGYKCNE 1174 HSP 4 Score: 103.99 bits (258), Expect = 1.143e-21 Identity = 81/247 (32.79%), Postives = 119/247 (48.18%), Query Frame = 3
Query: 2673 CMCKKGYSGQFCDQCETNHFGNAIS-------KDGTCQPCRC-----SGNIDQRI-EDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNG-----AVRTCTKCVCNHMGTNSKWQGICNH--------ETGKCPCLPNVVGLQCDRCQDKFFNLSSQ--IGCLPCNCDYPIGALNHS-CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNC 3326
C C G C++CE F N + K C+ C C S + D+ + S G C CL NT G +CE+C+ ++ + + R C C C+ G++ GIC+ G C C V G +C++C+D ++NL S GC PC C+ P+G LN+S C TG+C C+ +GK CN C +G GC+ C C+ GS C+ +G CRC+P +TGR+C
Sbjct: 318 CECTHNTKGMNCEECED--FFNDLPWKPAFGKKTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSD--DGICDSLNELEEGAVAGACHCKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCN-PLGTLNNSGCVMRTGECKCKKYVTGKDCNQCMPETYGLSESPEGCSLCNCDAGGSYDNYCDVISGQCRCRPHMTGRSC 559 HSP 5 Score: 102.834 bits (255), Expect = 2.282e-21 Identity = 85/268 (31.72%), Postives = 123/268 (45.90%), Query Frame = 3
Query: 921 EMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCD---SLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETG--CLKCNCVMMGTLENRG-CDSSTGKCY-CKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDN-KTCDKYSGKCLCKNGFAGIRCDE-IKSEWYI 1697
+++ G C C NT G+ C +C+ Y + P ECR C+CN H+++C DP+ G C +CQ +T +C+ C GYY N S C C C + G C LD N C C+E G++C+ C F+ GN + G C KC C L + G CD TG C C TG+ C CK G+F +A C C+C G+ + CD+++G+C C G+RCD+ ++ W I
Sbjct: 850 DLITGQCQCVPNTYGRECNQCQPGYWNFP----------ECRVCQCNGHAATC--DPIQ---------GTCIDCQDSTTGYSCDSCLDGYYGN--PLFGSEIGCRPCRCPETVASGLAHADGC----SLDTRNNNMLCHCQEGYSGSRCEICADNFF---GNPDNGGTCSKCECSNNVDLYDTGNCDRQTGACLKCLYQTTGDHCELCKDGFF--GDALQQNCQQCECDFLGTNNTIAHCDRFTGQCPCLPNVQGVRCDQCAENHWKI 1085 HSP 6 Score: 84.7297 bits (208), Expect = 8.602e-16 Identity = 69/223 (30.94%), Postives = 100/223 (44.84%), Query Frame = 3
Query: 2826 GECRKCLFNTSGFNCEKCVSGY--------FGNGAVRTCTKCVCNHMGTNSKWQ------------GICNHETGKCPCLPNVVGLQCDRCQDKFF-----NLSSQIGCLPCNCDYPIGALNH----SCNQL-----TGQCYCQAGRSGKMCNNCKDGFWG-DPTKLNGCTKCYCNLDGSVFKQ-CNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDC 3386
G C +C NT G NCE+C + FG C KC CN + + G+C++ CL N G C+ C F+ +++S+ C PC+CD P G+ + S N+L G C+C+A +G+ CN CKDG+W GC C CN G++ C G C+CK +TG++C+ CM T G + C C
Sbjct: 316 GRC-ECTHNTKGMNCEECEDFFNDLPWKPAFGK-KTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDN------CLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCD-PQGSSDDGICDSLNELEEGAVAGACHCKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCNPLGTLNNSGCVMRTGECKCKKYVTGKDCNQCMPETYGLSESPEGCSLC 529 HSP 7 Score: 82.0333 bits (201), Expect = 4.487e-15 Identity = 76/264 (28.79%), Postives = 121/264 (45.83%), Query Frame = 3
Query: 2493 GKCLNCKGNSFGYNCQNCENGYYGDTS-------RGILCKKCQCPGLSSLKNNSIGCSL---IFNTTAFTCM-----CKKGYSGQFCDQCETNHFGNA---ISKDGTCQPCRC--SGNIDQRIEDSCN-----SKTGECRKCLFNTSGFNCEKCVSGYFG--NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLS-SQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFW 3200
G+C C N+ G NC+ CE+ ++ D + CKKC+C ++++ C +F + F C GQ C++C + + I+ + CQPC C G+ D I DS N + G C C +G C +C GY+ + C C CN +GT + C TG+C C V G C++C + + LS S GC CNCD G+ ++ C+ ++GQC C+ +G+ C+ K ++
Sbjct: 316 GRC-ECTHNTKGMNCEECED-FFNDLPWKPAFGKKTNACKKCEC------NDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSDDGICDSLNELEEGAVAGACH-CKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCNPLGTLNN--SGCVMRTGECKCKKYVTGKDCNQCMPETYGLSESPEGCSLCNCDAG-GSYDNYCDVISGQCRCRPHMTGRSCSQPKQNYF 567 HSP 8 Score: 76.2554 bits (186), Expect = 2.810e-13 Identity = 65/260 (25.00%), Postives = 116/260 (44.62%), Query Frame = 3
Query: 2364 GKCLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTCD-----------SKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKN---NSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCS--GNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFG-NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFF 3050
G+C C +G +C+ C+ + + P+ C C+CN + +C G C NC N+ G +C+ C +Y D + I +C+ C C S + +S+ L A C CK +G+ C+QC+ ++ C+PC C+ G ++ C +TGEC KC +G +C +C+ +G + + C+ C C+ G+ + C+ +G+C C P++ G C + + +F
Sbjct: 316 GRCECTHNTKGMNCEECEDFFNDLPWKPAFGKKTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSDDGICDSLN-ELEEGAVAGACHCKAFVTGRRCNQCKDGYWNLQSDNPEGCEPCTCNPLGTLNN---SGCVMRTGEC-KCKKYVTGKDCNQCMPETYGLSESPEGCSLCNCDAGGS---YDNYCDVISGQCRCRPHMTGRSCSQPKQNYF 567 HSP 9 Score: 73.559 bits (179), Expect = 1.887e-12 Identity = 46/129 (35.66%), Postives = 69/129 (53.49%), Query Frame = 3
Query: 1311 NAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCL-CKNGFAGIRCDEIKSEWY 1694
N G+C CK NV G QCD+C G + F G E GC C+C +G+ +++ CD TG+C C G +C+ C+ GY+N C +C+C+ + + TCD G C+ C++ G CD +Y
Sbjct: 804 NGGYCQCKPNVVGRQCDQCAPGTYGF-GPE--GCKACDCNSIGS-KDKYCDLITGQCQCVPNTYGRECNQCQPGYWNFPE-----CRVCQCNGHAA----TCDPIQGTCIDCQDSTTGYSCDSCLDGYY 919 HSP 10 Score: 71.633 bits (174), Expect = 6.963e-12 Identity = 71/267 (26.59%), Postives = 112/267 (41.95%), Query Frame = 3
Query: 2214 IGGQCQCLPNVSGKTCERCAS--------ESYGFDARGCKKCKCD---LTGSLNSQCYNDTGKCL------CREGVEGQHCDTCKPGYWNFP--------FCHSCKCNQMSTT----CDS--------KTGKCLNCKGNSFGYNCQNCENGYY---GDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYF 2894
+ G+C+C N G CE C ++G CKKC+C+ ++ + + +G C GQHC+ C P ++ P C C C+ ++ CDS G C +CK G C C++GY+ D G C+ C C L +L N+ GC + C CKK +G+ C+QC +G + S +G C C C ++ C+ +G+CR C + +G +C + YF
Sbjct: 314 VHGRCECTHNTKGMNCEECEDFFNDLPWKPAFGKKTNACKKCECNDHAVSCHFDEAVFTASGFVSGGVCDNCLHNTRGQHCEECMPYFYRDPEQDITSERVCQPCDCDPQGSSDDGICDSLNELEEGAVAGAC-HCKAFVTGRRCNQCKDGYWNLQSDNPEG--CEPCTCNPLGTLNNS--GCVM----RTGECKCKKYVTGKDCNQCMPETYGLSESPEG-CSLCNCDAG--GSYDNYCDVISGQCR-CRPHMTGRSCSQPKQNYF 567 HSP 11 Score: 66.6254 bits (161), Expect = 2.787e-10 Identity = 59/209 (28.23%), Postives = 87/209 (41.63%), Query Frame = 3
Query: 933 GVC-DCQHNTKGQHCEKCKD-FYNDSPWLPADKNSTNECRKCECN---SHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNA--GHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYF 1538
G C C + T G HCE CKD F+ D+ C++CEC+ ++++ H D G C C N + C++C +++ I S + C C+CD +G L N+ G C CK G C++C+ +W GN C C C G + + CD TG C C + G C+ C GY
Sbjct: 1010 GACLKCLYQTTGDHCELCKDGFFGDA--------LQQNCQQCECDFLGTNNTIAHCDRFT---------GQCP-CLPNVQGVRCDQCAENHWK-----IASGEGCESCNCDPIGA-----------LHEQCNSYTGQCQCKPGFGGRACNQCQAHYW---GNPNEKCQPCECDQFGAADFQ-CDRETGNCVCHEGIGGYKCNECARGYI 1180 HSP 12 Score: 59.3066 bits (142), Expect = 3.594e-8 Identity = 55/194 (28.35%), Postives = 79/194 (40.72%), Query Frame = 3
Query: 1122 GGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCY-CKSFVTGNDCSTCKIGYFN--LSNANINGCSLCKCSEYGSRDNKTCDKYSGK-------CLCKNGFAGIRCD 1673
GG C C+ N + C++C G Y + C C C+S+G+K + L G C C N G +C++C+ G+W F C C C CD G C C+ TG C +C GY+ L + I GC C+C E + D S C C+ G++G RC+
Sbjct: 805 GGYC-QCKPNVVGRQCDQCAPGTYG------FGPEGCKACDCNSIGSK---------DKYCDLITGQCQCVPNTYGRECNQCQPGYWNF-----PECRVCQCNGHAAT----CDPIQGTCIDCQDSTTGYSCDSCLDGYYGNPLFGSEI-GCRPCRCPETVASGLAHADGCSLDTRNNNMLCHCQEGYSGSRCE 972 HSP 13 Score: 58.5362 bits (140), Expect = 6.161e-8 Identity = 34/90 (37.78%), Postives = 48/90 (53.33%), Query Frame = 3
Query: 2169 CKCNVTGSRNNN-CNKIGGQCQCLPNVSGKTCERCASESYGFD--ARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYW 2429
C CN G+ NN+ C G+C+C V+GK C +C E+YG GC C CD GS ++ C +G+C CR + G+ C K Y+
Sbjct: 478 CTCNPLGTLNNSGCVMRTGECKCKKYVTGKDCNQCMPETYGLSESPEGCSLCNCDAGGSYDNYCDVISGQCRCRPHMTGRSCSQPKQNYF 567
BLAST of Netrin-4 vs. Ensembl Fly
Match: LanB2 (gene:FBgn0267348 transcript:FBtr0345031) HSP 1 Score: 347.051 bits (889), Expect = 8.460e-97 Identity = 343/1322 (25.95%), Postives = 553/1322 (41.83%), Query Frame = 3
Query: 192 LRASSTCGLTNSERYCVVTTSDSGRKSKCTIC--DSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQS---MSGVHN---VSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPE----RKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNA-GHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENR-GCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQ-FFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEK-----DLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARG-----CKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF------CHSCKC--NQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL-----CKKCQCPGLSSLKNNSIG-CSLIFNTTAFTCM-CKKGYSGQFCDQCETNHFGNAIS-KDGTCQPCRCSGNIDQRIEDS---CNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNL--------DGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSS-----CGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEI----KKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRN---INLKFIRQK-------SKDMDNLINEMSKNVITF 3944
+ +++TCG N +C+ T + + + C C + NP + H PQ P WWQS G+ + V++ + + T+ + F++ RP + I + W Y ++S C RD S+P+ RK LCTSE+S I P G + FS + + + + T+IR+ RL+TFGD D+ +++ Y+YAIS+ + RC C GHASKC G + +V C+C+HNT G C++C YND W + NEC+ C CN + C FD ++ +R GG C +C+ N + NCERCK +Y + C+ C CD +G++ L N+ G C CK V G++CD+C +++F + GC +C C G+ +N CD+ TG C+CK V G C+ CK G+FNL N GC+ C C YG ++ +C+ G++ + + S + K + L + +++ + + Y + TT N +F + L + ++ +QL+ + + LE LPI I + S R L +NL + K + A S D ++ + G+ + I QC C G+ CE CA ARG C C C + C ++TG+C+C+ G +CD C G++ C C C + + T C C FG C+ C +G++GD + G+L CK C C G ++ N++G C N T C+ C +G+ CDQC + HFG+ ++ G C C C ++ E S C+ TG+C +C N G +C +C GYF + C C+C+ +G+ + C+ +G+C C P V+G +CD+C++ F+ SS+ GC PC CD G+ C+Q GQC C G+ C+ CK+ + C CY NL +F + + R P + +K K+ + GD + + + D + +S KF + + QN L +I KK D++ ++ L+R K ++F Q I I A+A+ K + +E + LKN + +K +Q D ELR+ + L ++Q SK+ NE+ +T
Sbjct: 80 IESTNTCGEQNDNHFCIQTMNQNHKN--CEFCKYNDHNP-SFLTDLHD----------PQSP-TWWQSETMFEGIQHPNYVNLTLHLGKSYDITYVRILFRSPRPESFTIYKRTSESGPWIPYQFYSATC-RDTYSLPDSRAIRKGEGEAHALCTSEYSDISPLRDGEIAFSTLEGRPSGINFERSGELQEWVTATDIRITLDRLNTFGDELFGDSQVLKS-YFYAISDIAVGARCKCNGHASKCVPSTGMHGERTLV---CECRHNTDGPDCDRCLPLYNDLKWKRSTSTEVNECKACNCNGLADKCFFDANLF--NRTGHGGHCLDCRENRDGPNCERCKENFY------MRDDGYCVNCACDPVGSR-----------SLQCNSHGKCQCKPGVTGDKCDRCDNNYYQFGPH---GCQQCGCDSGGSHQNTPACDTETGICFCKENVEGRRCNECKPGFFNLDKNNRFGCTPCFC--YG---------HTSECMTAPGYSIV---SVTSNFNKFKERWTAADLNQREVD---IKYNQYSRSIGTTAQGNEHVYFQAPDRFLGDQRASYNRDLKFK--LQLVGQVANTGVSDVILEGAGSRISLPIFAQGNGIPDQGVKEYTFRLHEHHDYQWQPSQSARGFLS---ILSNLTAIK----IRATYSVQGEAILDDVELQTAHRGAAGHPATWI-EQCTCPEGYLGQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADI---CDSETGRCICQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPT-GLLGEVQTCKSCDCNG--NVDPNAVGNC----NRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQC-QCKPNVIGRDCGECQPGYFNIRSGNGCENCLCDPVGS---YNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSE-GCKPCECDES-GSKGFQCDQ-NGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDCY-NLVQDAADLHRAKLFNLSQTLDEIAR-TPVTNDDEFEAKLKAVQEKVAVLAQDARDNSGDGGQTYAEVIDDLHKHLDSVREH---LVSADKFQADANGEIDRARQNYTILDQITENAKKELQQALDLLNDEGAQALARAK-----EKSVEFGQQSEQISDISRE---ARALADKLE--------SEAQFDLKNAKDAKDAVEKAHQLAKSAIDLQLKIGTELRSEVGLELSHVKQSLGTVVQTSKEALRKANEVYDTALTL 1295 HSP 2 Score: 238.039 bits (606), Expect = 1.132e-62 Identity = 183/622 (29.42%), Postives = 282/622 (45.34%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWNFPF-------CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDG---TCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRT---CTKCVCNHMGTNSKWQGI--CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFD----KLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIG-----------YDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASE-SKKIYQNITLIKD------KLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLD 4115
+C C EG GQ C++C PGY + P C C C+ + CDS+TG+C+ C+ N+ G NC C G+YG+ G CK+C CP N C I T C KGY G C+QC FG+ G TC+ C C+GN+D +CN TGEC KC+ NT+G +C++C+SG+FG+ C +C C GT Q I C+ TG+C C PNV+G C CQ +FN+ S GC C CD P+G+ N +C++ +GQC+C+ G G+ C+ C++ F+G ++ GC C C+ GS QC++ NG C C + GR CD C + + C C DC+ +V++ D KL N S + P DD E +K+++E Y +I + + L ++ ++ +++ F N I I++N +++ A + L L A E S + Q I D L + E +LK + +KD ++++K+ I + L S +G +K + K+N+ ++ +LL D
Sbjct: 709 QCTCPEGYLGQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADICDSETGRCI-CQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCP-------NDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGNGCENCLCD-PVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSE--GCKPCECDESGSKGFQCDQ-NGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDCY-------NLVQDAADLHRAKLFNLSQTLDEIARTPVTNDD---EFEAKLKAVQEKVAVLAQDARDNSGDGGQTYAEVIDDLHKHLDSVREHLVSAD--KFQADANGEIDRARQNYTILDQITENAKKELQQALDLLNDEGAQALARAKEKSVEFGQQSEQISDISREARALADKLESEAQFDLKNAKD-AKDAVEKAHQLAKSAIDLQLKIGTELRSEVGLELSHVKQSLGTV-------VQTSKEALRKANEVYDTALTLLND 1298
BLAST of Netrin-4 vs. Ensembl Fly
Match: LanB2 (gene:FBgn0267348 transcript:FBtr0076382) HSP 1 Score: 347.051 bits (889), Expect = 8.460e-97 Identity = 343/1322 (25.95%), Postives = 553/1322 (41.83%), Query Frame = 3
Query: 192 LRASSTCGLTNSERYCVVTTSDSGRKSKCTIC--DSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQS---MSGVHN---VSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPE----RKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNA-GHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENR-GCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQ-FFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEK-----DLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARG-----CKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF------CHSCKC--NQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL-----CKKCQCPGLSSLKNNSIG-CSLIFNTTAFTCM-CKKGYSGQFCDQCETNHFGNAIS-KDGTCQPCRCSGNIDQRIEDS---CNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNL--------DGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSS-----CGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEI----KKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRN---INLKFIRQK-------SKDMDNLINEMSKNVITF 3944
+ +++TCG N +C+ T + + + C C + NP + H PQ P WWQS G+ + V++ + + T+ + F++ RP + I + W Y ++S C RD S+P+ RK LCTSE+S I P G + FS + + + + T+IR+ RL+TFGD D+ +++ Y+YAIS+ + RC C GHASKC G + +V C+C+HNT G C++C YND W + NEC+ C CN + C FD ++ +R GG C +C+ N + NCERCK +Y + C+ C CD +G++ L N+ G C CK V G++CD+C +++F + GC +C C G+ +N CD+ TG C+CK V G C+ CK G+FNL N GC+ C C YG ++ +C+ G++ + + S + K + L + +++ + + Y + TT N +F + L + ++ +QL+ + + LE LPI I + S R L +NL + K + A S D ++ + G+ + I QC C G+ CE CA ARG C C C + C ++TG+C+C+ G +CD C G++ C C C + + T C C FG C+ C +G++GD + G+L CK C C G ++ N++G C N T C+ C +G+ CDQC + HFG+ ++ G C C C ++ E S C+ TG+C +C N G +C +C GYF + C C+C+ +G+ + C+ +G+C C P V+G +CD+C++ F+ SS+ GC PC CD G+ C+Q GQC C G+ C+ CK+ + C CY NL +F + + R P + +K K+ + GD + + + D + +S KF + + QN L +I KK D++ ++ L+R K ++F Q I I A+A+ K + +E + LKN + +K +Q D ELR+ + L ++Q SK+ NE+ +T
Sbjct: 80 IESTNTCGEQNDNHFCIQTMNQNHKN--CEFCKYNDHNP-SFLTDLHD----------PQSP-TWWQSETMFEGIQHPNYVNLTLHLGKSYDITYVRILFRSPRPESFTIYKRTSESGPWIPYQFYSATC-RDTYSLPDSRAIRKGEGEAHALCTSEYSDISPLRDGEIAFSTLEGRPSGINFERSGELQEWVTATDIRITLDRLNTFGDELFGDSQVLKS-YFYAISDIAVGARCKCNGHASKCVPSTGMHGERTLV---CECRHNTDGPDCDRCLPLYNDLKWKRSTSTEVNECKACNCNGLADKCFFDANLF--NRTGHGGHCLDCRENRDGPNCERCKENFY------MRDDGYCVNCACDPVGSR-----------SLQCNSHGKCQCKPGVTGDKCDRCDNNYYQFGPH---GCQQCGCDSGGSHQNTPACDTETGICFCKENVEGRRCNECKPGFFNLDKNNRFGCTPCFC--YG---------HTSECMTAPGYSIV---SVTSNFNKFKERWTAADLNQREVD---IKYNQYSRSIGTTAQGNEHVYFQAPDRFLGDQRASYNRDLKFK--LQLVGQVANTGVSDVILEGAGSRISLPIFAQGNGIPDQGVKEYTFRLHEHHDYQWQPSQSARGFLS---ILSNLTAIK----IRATYSVQGEAILDDVELQTAHRGAAGHPATWI-EQCTCPEGYLGQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADI---CDSETGRCICQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPT-GLLGEVQTCKSCDCNG--NVDPNAVGNC----NRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQC-QCKPNVIGRDCGECQPGYFNIRSGNGCENCLCDPVGS---YNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSE-GCKPCECDES-GSKGFQCDQ-NGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDCY-NLVQDAADLHRAKLFNLSQTLDEIAR-TPVTNDDEFEAKLKAVQEKVAVLAQDARDNSGDGGQTYAEVIDDLHKHLDSVREH---LVSADKFQADANGEIDRARQNYTILDQITENAKKELQQALDLLNDEGAQALARAK-----EKSVEFGQQSEQISDISRE---ARALADKLE--------SEAQFDLKNAKDAKDAVEKAHQLAKSAIDLQLKIGTELRSEVGLELSHVKQSLGTVVQTSKEALRKANEVYDTALTL 1295 HSP 2 Score: 238.039 bits (606), Expect = 1.132e-62 Identity = 183/622 (29.42%), Postives = 282/622 (45.34%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWNFPF-------CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDG---TCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRT---CTKCVCNHMGTNSKWQGI--CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFD----KLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIG-----------YDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASE-SKKIYQNITLIKD------KLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLD 4115
+C C EG GQ C++C PGY + P C C C+ + CDS+TG+C+ C+ N+ G NC C G+YG+ G CK+C CP N C I T C KGY G C+QC FG+ G TC+ C C+GN+D +CN TGEC KC+ NT+G +C++C+SG+FG+ C +C C GT Q I C+ TG+C C PNV+G C CQ +FN+ S GC C CD P+G+ N +C++ +GQC+C+ G G+ C+ C++ F+G ++ GC C C+ GS QC++ NG C C + GR CD C + + C C DC+ +V++ D KL N S + P DD E +K+++E Y +I + + L ++ ++ +++ F N I I++N +++ A + L L A E S + Q I D L + E +LK + +KD ++++K+ I + L S +G +K + K+N+ ++ +LL D
Sbjct: 709 QCTCPEGYLGQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADICDSETGRCI-CQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCP-------NDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGNGCENCLCD-PVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSE--GCKPCECDESGSKGFQCDQ-NGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDCY-------NLVQDAADLHRAKLFNLSQTLDEIARTPVTNDD---EFEAKLKAVQEKVAVLAQDARDNSGDGGQTYAEVIDDLHKHLDSVREHLVSAD--KFQADANGEIDRARQNYTILDQITENAKKELQQALDLLNDEGAQALARAKEKSVEFGQQSEQISDISREARALADKLESEAQFDLKNAKD-AKDAVEKAHQLAKSAIDLQLKIGTELRSEVGLELSHVKQSLGTV-------VQTSKEALRKANEVYDTALTLLND 1298
BLAST of Netrin-4 vs. Ensembl Fly
Match: wb (gene:FBgn0261563 transcript:FBtr0302731) HSP 1 Score: 265.388 bits (677), Expect = 9.658e-71 Identity = 318/1270 (25.04%), Postives = 498/1270 (39.21%), Query Frame = 3
Query: 192 LRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR--WWQSMS-----GVHNVSIEISFESEFQFTFTHLR-FKTYRPAAMYIERSKDYGRTWNKYAYF--SD-NCPRDFPSIPERKR---SNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITN--IRVQFVRLHTFG------DWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLG---LNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLI---------KDSRYIVIPNKCL--EKDLPIVLTVFTIESN--NKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRN----NNCNKIGGQC-----QCL--PNVSGKTCERCASESYGF--------DARGCKKC-KCDLTGSLNSQCYNDTGKCL-CREGVEGQHCDTCKPGYWNFPF------CHSCKC------NQMSTTCDSKTGK-----------------CLNCKGNSFGYNCQNCENGYYGDTSR-GILCKKCQCPG--------------------------LSSLKNNSIGC-----------SLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVR-----------------------------------TCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGF----------------------------------------------WGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCG 3380
+ ++TCG +E YC + C IC++ + + + I + F WWQS + V+I + + FQ L+ + RPA+ +E+S D G + + YF SD +C R + + + N E++C+++FS P E G + S N ++ + IT IR++ +H+ DW +D +P++ +Y++S+ + R C GHA++ ++ C CQHNT G CE+C + D P+ EC C+C H+ SC +DP + G+C +C +NT CE C+ G+YR + + + CL C C+ P G + G C C E +G C++C G++ G++ C +C C G+L + G S +G C CK V G+ CS C GYF+LS N GC+ C CS ++TC + L E ++W I ++ +K I I P +++ F D ++ + S+ ++G + SY +QL+ + + PN L + L I +++ N V KTR E +Y S + FL+ +S + V T + ++GG+ QCL +G +CE CA +GF D + KC C G NS C +G C C G C+ C+ GY+ P C C C N S +C K+ C C G +CQ C++GY+G+ + G C++C C G L + ++GC S + ++T C+CK Y+GQ CD+C+ + + C C C + ++D C+ TG+C C G C +C GYFG AV C +C C+ +G+ S C+ TG+C CL NV G +CD+C+ +NL++ GC C CD P G+ H CN TGQC C+ G G+ CN C +GF WG +L GC +C C+ GS+ +QC+ +G C+C+ G +GR CD C G G P C CG
Sbjct: 166 ISVNATCGQNGAEEYCKQVGA-----KPCGICNAHSSDRAKQRSIQSLISSGSGSGSGSGFEEGWWQSPTLQGGRQFEYVTILLDLKQTFQIFSVWLKSANSPRPASWILEKSLD-GINFEPWQYFGLSDADCQRRWNLSGQNGKYVFQNDTEIICSTQFSKPGPLENGVL----HASLLKNRPGATDQSPELMKFITTRYIRIRLQGMHSTANQDNSLDWLLD-SPSLEKHSFYSLSQLKVSARLDCNGHANRSHESPDDPLMQ------CICQHNTCGAQCEQCCPLFQDRPY-----QMGGECEICQCYGHAESCTYDPF-------LDKGICQSCSNNTAGIECEFCEMGFYRELDAPL--TDPCLPCSCN-------------PARSTGGCQSDGGSCNCLEGFQGKNCEECAPGYY---GDD---CKRCECDERGSLGSTG--SCSGVCQCKLNVEGSTCSECAPGYFDLSAENAEGCTSCWCSGV----SQTCHSAKLQTLAF---------ETLNDWKITD----IQRVKPISI--PVDAETNRLIFANELDEVEAIYWQA-----SLGYLG-NRLTSYGSRLQLVLSWDVIRGDRSGKPTTGPNVILVGKNGLKIAFGDESLDGLGINLNVTLTEVGWYHVPPTVVDIKTRLRRTEGGDYHG-ESVTRSQFLSVLVSLDAVLIRAAFHTDQGETSLERAVIYSGGVELGGKSSSQVEQCLCPAGYTGLSCEGCA---FGFKRIYENTSDHQILSKCIPCPCNGHSNS-CDLQSGNCGDCMHNTFGDRCERCQLGYYGNPLQGTPNDCKRCACPLSEDSNNFSPSCQLKSYNYMDLNPQFELIEHAEYICTQCPEGYTGDHCQVCDDGYFGNPRQPGSSCQRCDCAGGPCNVTTGECITCRGNTEGWHCERCKLGYWGDPAVGCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYANVELR----CPSCNCD-PLGSLVQDRCDPHTGQCH-CKEGVMGAKCHECQDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEECHCSSVGSLSS---DCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCD-PHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARL-GCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFG-YPECRRCG 1341 HSP 2 Score: 171.014 bits (432), Expect = 4.844e-42 Identity = 131/429 (30.54%), Postives = 193/429 (44.99%), Query Frame = 3
Query: 2205 CNKIGGQC-QCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF-CHSCKCNQMSTT----CDSKTGKCLNCKGNSFGYNCQNCENGYYG----------------------------------DTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKC-VCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCN-----RNNGVCRCKPGITGRNCDMCMKGTDG 3353
CN G+C C N G CERC +G A GC C C GS + C + G+CLC+ GQ CD C GY N C SC C+ + + CD TG+C +CK G C C++GY+G ++ + C++C C + SL ++ + C C +G+ CD+C H+ N + +G C+ CRC + + E CN TG+C C G +C +C G+FG + C +C C G +C+ G+C C GL C +C + +++GC C CD+ IG++ C+ GQC C+ G SG+ C+ C G++G P C +C C+ +GS F Q + +NG C CK + G CD CM+ T G
Sbjct: 981 CNVTTGECITCRGNTEGWHCERCKLGYWGDPAVGCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYANVELRCPSCNCDPLGSLVQDRCDPHTGQC-HCKEGVMGAKCHECQDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEECHCSSVGSLSSDC-------DKRTGQCACLANVTGRRCDKCRPGHW-NLTAGEG-CRDCRCDPHGSRGHE--CNPWTGQC-DCKIGVGGQHCNECTEGFFGF-STEGCQRCSACRSEGQ------VCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDH-IGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPE----CRRCGCDAEGS-FTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFG 1383 HSP 3 Score: 130.568 bits (327), Expect = 1.001e-29 Identity = 115/409 (28.12%), Postives = 163/409 (39.85%), Query Frame = 3
Query: 2157 DPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSL-NSQCYNDTGKCLCREGVEGQHCDTCKPGYWNF-----------------------------------------PFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLT----GQCYCQAGRSGKMCNNCKDGFWGDPTK-LNGCTKCYC 3242
DP C C+ GS + C+ GQC C P +G+ C+ C + R C C CD GSL +C TG+C C+EGV G C C+ GY+ CH +S+ CD +TG+C C N G C C G++ T+ G C+ C+C S + N C CK G GQ C++C FG + C CR G + C+ G C C T G C +CV G +G A C +C C+H+G+ + C+ G+C C G +CD C +F C C CD G+ + + GQC C++ G C+ C +G + GCT+C+C
Sbjct: 1016 DP--CHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYANVELR-CPSCNCDPLGSLVQDRCDPHTGQCHCKEGVMGAKCHECQDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEECHCSSVGSLSSDCDKRTGQCA-CLANVTGRRCDKCRPGHWNLTA-GEGCRDCRCDPHGSRGHEC-------NPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQV-------CDPHNGRC-ICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQ---CSTGDGQCQCREGYSGRKCDTCAIGYFGYPE---CRRCGCDAE-GSFTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFC 1397 HSP 4 Score: 95.1301 bits (235), Expect = 5.465e-19 Identity = 53/144 (36.81%), Postives = 83/144 (57.64%), Query Frame = 3
Query: 2451 CKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAIS-KDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKC 2879
C CN S+ CD +TG C NC+GN+ G +C C G+YGD + C+ C CP + +N + GC++ +C+CK GY+G+ C++C+ +FG+ + + TCQPC C + D + C+ +TG C C +G C+KC
Sbjct: 1670 CHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCP--ETNRNFARGCNVW--DGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNC--HPDGIQTEGCDVETGRC-YCREGVTGLKCDKC 1806 HSP 5 Score: 88.1965 bits (217), Expect = 8.480e-17 Identity = 65/214 (30.37%), Postives = 91/214 (42.52%), Query Frame = 3
Query: 2169 CKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKC-KCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF---CHSCKCNQMSTT---CDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSL--KNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSG 2783
C+C+ GSR + CN GQC C V G+ C C +GF GC++C C G + C G+C+C + G C C PG W + C C+C+ + + C + G+C C+ G C C GY+G C++C C S + SI C + C CK G CD C + FG + C C C G
Sbjct: 1199 CRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQV---CDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQC-QCREGYSGRKCDTCAIGYFGYPE----CRRCGCDAEGSFTQADGSIACD-----SNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFG 1399 HSP 6 Score: 85.5001 bits (210), Expect = 5.345e-16 Identity = 55/158 (34.81%), Postives = 78/158 (49.37%), Query Frame = 3
Query: 2172 KCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDAR-GCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQM--------STTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL-CKKCQCPGLS 2615
+C+ S C+ G+C C G C +C ++G+ AR GC++C+CD GS+ QC G+C CREG G+ CDTC GY+ +P C C C+ S CDS G+C CK G C C +G +++ C +C C G S
Sbjct: 1246 RCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDS-NGQC-PCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRS 1401 HSP 7 Score: 77.411 bits (189), Expect = 1.261e-13 Identity = 58/197 (29.44%), Postives = 88/197 (44.67%), Query Frame = 3
Query: 2880 VSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCP-CLPNVVGLQCDRCQDKFF-NLSSQIGCLPCNCDYPIGALNHSCNQLTGQ--CYCQAGRSGKMCNNCKDGFWGDPTKL--NGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPN-CSSCGDCWLQWDHFLIVVKNTFDK-LHN 3446
V F + C CN ++ C+ ETG C C N G C +C + F+ + +S GC C C CN G+ C C+ G +G++C C+ G++GDP + C C C+ DG + C+ G C C+ G+TG CD C + N C C +C L ++ +V N + +HN
Sbjct: 1655 VESVFIEDLIGRAAPCHCNGRSSD------CDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKICDNCTLLLLDYMELVGNKLRRGMHN 1845 HSP 8 Score: 76.2554 bits (186), Expect = 2.973e-13 Identity = 68/251 (27.09%), Postives = 103/251 (41.04%), Query Frame = 3
Query: 933 GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHF-DPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGS---RDNKTCDKYSGKCLCKNGFAGIRCD 1673
G C C N G+ C+KC+ + W + + CR C C+ H S H +P G CD C+ ++C C G++ + Q C C + +C +P + G CIC + G C +C G W + GC +C C +G++ + C + G+C C+ +G C TC IGYF C C C GS D +G+C CK+ G++CD
Sbjct: 1167 GQCACLANVTGRRCDKCRPGH----W---NLTAGEGCRDCRCDPHGSRGHECNPWT---------GQCD-CKIGVGGQHCNECTEGFF---GFSTEGCQRCSACRSEG------QVC--DP------HNGRCICPKFTRGLGCGQCVPGTWGWQA--RLGCRECECDHIGSIGQQ-CSTGDGQCQCREGYSGRKCDTCAIGYFGYPE-----CRRCGCDAEGSFTQADGSIACDSNGQCPCKSLVVGLKCD 1375 HSP 9 Score: 75.8702 bits (185), Expect = 4.564e-13 Identity = 98/414 (23.67%), Postives = 151/414 (36.47%), Query Frame = 3
Query: 390 MSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGR-------TWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDI-TAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCE-KCKDFYN-------DSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSIC-IQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGN-EETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLC 1577
+S V + +S + QF + L F+ P Y +R+ YG T + N +P + S + +L E+ S T S W + RN + A+ NIR F+R F D++ N+ H +G + C C G C+ + FY +S ++ ++ C CN SS C + GVC NC+ NT +C +C G+Y + NS C C C F C + + E+ C+CK G C++C+ G++ T C CNC G ++ GCD TG+CYC+ VTG C C+ +L + NGC +C
Sbjct: 1463 LSLVPKSTGNVSIGAYGQF-YHPLYFQL--PPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKL--LLDFYEYEEFEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNV---------------------HMDAAIYIKGSTNLIAKGVERCKCPKRFDGLSCQDPGRSFYRWRNTTIVESVFI---EDLIGRAAPCHCNGRSSDCDRET-----------GVCQNCRGNTGGDHCHQCAEGFYGDP----NSPHGCQACPCPETNRNFARGCNVWDGEVS-------CVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDG-IQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVD---NGCKIC 1821 HSP 10 Score: 69.707 bits (169), Expect = 3.489e-11 Identity = 69/222 (31.08%), Postives = 90/222 (40.54%), Query Frame = 3
Query: 933 GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCE-CNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENR----GCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKC 1583
G CDC+ GQHC +C + + ST C++C C S C DP G C C T C +C G + + C +C CD +G SI Q D G C C+E G +CD C IG++ + C +C C G+ CDS+ G+C CKS V G C TC F LS N GC+ C C
Sbjct: 1216 GQCDCKIGVGGQHCNECTEGFFGF--------STEGCQRCSACRSEGQVC--DP---------HNGRCI-CPKFTRGLGCGQCVPGTW-----GWQARLGCRECECDHIG----SIGQQCSTGD-----GQCQCREGYSGRKCDTCAIGYFGY-----PECRRCGCDAEGSFTQADGSIACDSN-GQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFC 1397 HSP 11 Score: 61.6178 bits (148), Expect = 9.401e-9 Identity = 41/123 (33.33%), Postives = 59/123 (47.97%), Query Frame = 3
Query: 1329 CKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGK--CYCKSFVTGNDCSTCKIGYFNLSNANING-CSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSE 1688
C+ N G+ C +C GF+ + N GC C C RGC+ G+ C CK TG C C+ GYF N C C C G + + CD +G+C C+ G G++CD+ ++E
Sbjct: 1689 CRGNTGGDHCHQCAEGFYG-DPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQ-TEGCDVETGRCYCREGVTGLKCDKCQAE 1809 HSP 12 Score: 58.5362 bits (140), Expect = 8.010e-8 Identity = 44/135 (32.59%), Postives = 62/135 (45.93%), Query Frame = 3
Query: 2997 CLPNVVGLQCDRCQDKFF-NLSSQI--GCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGT-DGKMPNCSSCGDCW 3389
C N G++C+ C+ F+ L + + CLPC+C+ P + C G C C G GK C C G++GD C +C C+ GS+ +GVC+CK + G C C G D N C CW
Sbjct: 475 CSNNTAGIECEFCEMGFYRELDAPLTDPCLPCSCN-PARSTG-GCQSDGGSCNCLEGFQGKNCEECAPGYYGD-----DCKRCECDERGSL-GSTGSCSGVCQCKLNVEGSTCSECAPGYFDLSAENAEGCTSCW 601 HSP 13 Score: 54.6842 bits (130), Expect = 9.961e-7 Identity = 42/142 (29.58%), Postives = 64/142 (45.07%), Query Frame = 3
Query: 2169 CKCNVTGSRNNNCNKIGGQCQ-CLPNVSGKTCERCASESYGF--DARGCKKCKCDLTG---SLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF------CHSCKCNQ---MSTTCDSKTGKCLNCKGNSFGYNCQNCE 2549
C CN R+++C++ G CQ C N G C +CA YG GC+ C C T + ++ C+C+ G G+ C+ C+ GY+ P C C C+ + CD +TG+C C+ G C C+
Sbjct: 1670 CHCN---GRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCY-CREGVTGLKCDKCQ 1807
BLAST of Netrin-4 vs. Ensembl Zebrafish
Match: lamb2 (laminin, beta 2 (laminin S) [Source:ZFIN;Acc:ZDB-GENE-081030-4]) HSP 1 Score: 855.899 bits (2210), Expect = 0.000e+0 Identity = 549/1805 (30.42%), Postives = 870/1805 (48.20%), Query Frame = 3
Query: 87 IFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYI----------------------EKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQF--------------FDSFIIVLSIYHVGKSS-CQSYQD-----TMQLIKDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXX----------------XXXXXXXNSTKTRKVLGE---CLNYANLVSSKKCDFLNAEI--STNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI--LCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTN-----SKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNM-KSLKEIKKYFPIGYDII---------ISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMIS--KNKIRDVTGAFNELKKSLKNILN----GASESKKIYQN-----------ITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVIT-FNGVTCN-TITESNPLMCS---------EFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTN---------MSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLL----NKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLS---KDIDE-------KLSRLLVLQDKI 5090
+ A E ++ + C C P GDLL+GRE+ L+ASSTCGL E YC+V S + KC CDS+ PY S+ SHRIENV++ P WWQS +G +V +++ E+EF FT + FKT+RPAAM IERS D+GR+W Y YF+++C FP I + +++V+C S +S I PS G V+F D Y + +N + ITN+R+ +LHT GD +D P I+ KYYYA+ E ++RG C CYGHAS+C EG + ++ MV+G C C+HNTKG +CE+C DFYND PW PA+ +TN C+KC CN HS+ CHFD VY + +SGGVCDNC HNT NCE CK YY++ I C+ C CD G+K +C + LG+ AG C CK+NVEG++CDKCK GF+ + ++ GC C C GT+ CD +G C+CK VTG+ C+ C ++ LS+ + +GC C C G+ +N+ C +G+C C++ G +C++++S ++ E+ P + + G F I +M++ + ++V+ + SS C + T+ L +R++V+P CLE + L V I ++ + ++ RK E C A VS + + A++ S + + + C+C+ GS ++ C+ GGQC+C PN+SG+ CE+CA +YGF GCK C+C L GS + C +G+C CR G GQ CD C+ G+W FP C C+CN + C +TG CLNC+ N+ G C+ C NGYYG+ G+ C+ C CP G +S ++ + C + C C +GY+G CD+C +FG+ G CQPCRCS NID +SC+ +TG+C KCL+NT G +C C SGY+G+ + R C KC CN +GT S+ +C TG+C CLPNV+G CD C +NL+S GC PC CD P A +CN+ TGQC C+ G GK C +C++ WGDP L C C C+ G QCNR G C C+ G++G CD C +G G P+C C C+ WD + + L + + + T L + + KE++ DI+ ++ + L+ + + N Q+ + +++S A +S + + +++ ++L + L + N GA +S + N T ++ + R + I QK D N N +K +T N N + + N +C CGG GC+ D+ R C G + A LDR+ M ++ + F Q + ++E KD + + T +D + ++ +L+ +I+ +L AD E++ + + I +I L +I+ R++ L+NV+ IL++++ + ++ + A +A+ + N+ +++ + + ++ + N + L N ++E A ++ +++ +R+ L+ I + +N L + + + + + K + ++S++++++ NK+ + D + K L+ D + KLQ L+ KD +E K +L L+DK+
Sbjct: 11 VLSAVSAQEPDAAHGCTHGSCYPATGDLLVGREANLKASSTCGLRRREPYCIV--SHLQEEKKCFQCDSRRPYDPQSNPVSHRIENVITTFKPHRKKSWWQSENGKSDVYLQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRSWQVYRYFANDCESIFPGISQDSLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLK-ITNLRLNLTKLHTLGDNLLDSRPEIKEKYYYAMYELVVRGNCFCYGHASECAPIEGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFYNDLPWRPAEGRNTNACKKCNCNGHSNQCHFDMAVYLATGNISGGVCDNCLHNTMGSNCESCKPFYYQDPTRDIRDPGVCVACDCDPDGSKNGGVCDGHDDPSLGMIAGQCRCKDNVEGSRCDKCKPGFFGLSASDPRGCQPCKCDPRGTVSGSSQCDPVSGDCFCKRLVTGHSCNQCLPEHWALSH-DTSGCRGCDCDVGGATENQ-CSMETGQCQCRSHMIGRQCNQVESGYFFMALDHYIYEAETAKLGQGSVFEEREYQPGRPISWTGTGFARAPEGGTLEFAINNIPYSMEYDVLIRYELQMPRDWEEVRVVVIRPGPIPTSSPCGNTIPADDLLTVSLSSGARFVVLPQPVCLESGVSYKLRVELIRYADRNSIITSTNAFVLVDSIALLPRYLSLEMFTAGDPASVARKQSFERYRCHETAKSVSRPQMSEVCAKLITSMSALINDGALACECDPQGSLSSECDVRGGQCRCRPNISGRRCEKCAPGAYGFGPSGCKLCECSLEGSQSRFCDLYSGQCPCRPGAYGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLNCRSNTAGDKCERCANGYYGNPVLGLGSQCRPCPCPEGPNSGRHFAASCYQDNRSQQVVCNCNQGYTGPRCDECAPGYFGDPSKPGGQCQPCRCSNNIDLSDPESCDKRTGQCLKCLYNTEGPDCSVCRSGYYGDASRRNCRKCTCNFLGTERSQCLSRDDCVCQRATGQCQCLPNVIGQTCDHCAPNSWNLASGQGCEPCACD-PNNAYTSACNEFTGQCQCRVGFGGKTCTDCQENHWGDPRVL--CRACDCDPRGIESSQCNRMTGHCVCQQGVSGVRCDQCARGFSGTFPDCKPCHQCFGDWDRIVQDLATRTKALTDRAKELQT------------TGLTRAFERRFKELEDMLAQARDIVNARNATAEAVTTLMGMIEVLRAQIGETTDT-LNQQEGDLTAVQDSNYEASNALSTLEREAKELNLNADQLNRQLDILKNSNFLGAYDSIRASYNKSRDAEHRSNRSTTDTPSTVSQSADTRKKTERLIGQKRDDF-NRKNAANKRTLTDLNAKVQNLDLKKINEKVCGAPGDAQCVDSPCGGAGCRDDEGKRR------CGGLNCNGAVAVADTALDRSKHAEKELDKAMGVVEELFKQVADAKTKAQEAKDRAQAALEKASDTKNKVDHSNNDLR-------DLIKQIRQFLMQEGADPDSIEAVANRVLELSIPASPKQIRHLADEIKDRVKSLSNVDAILEQTQNDVRKAEQLLLDAKKARNKAEGVKNTAESVKKALNDASRAQAAAEKAIQKAKNDI-GLTQNQLAQIQSETSASER--DLNDAVDRLGDLERQIEALKTKRANN-------------SLDAARAEETATMARDKANEAKEILDGELSDKYRTVQGLMDNKAKTMQDAKHKAEQLRDEAKGLLKDAQDKLQRLAELEKDYEENQKVLEGKARQLDGLEDKM 1764
BLAST of Netrin-4 vs. Ensembl Zebrafish
Match: lamb1a (laminin, beta 1a [Source:ZFIN;Acc:ZDB-GENE-021226-1]) HSP 1 Score: 799.275 bits (2063), Expect = 0.000e+0 Identity = 557/1800 (30.94%), Postives = 885/1800 (49.17%), Query Frame = 3
Query: 63 MTLNYVFIIFVARYILE--IESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFS-STHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE-MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLEN-RGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCK------------NGFAGIRCDEIKSEWYIEK-----PTYPLEHLKEIK--------INLPP----------VSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHV--GKSSCQSYQ--DTMQLI---KDSRYIVIPNK-CLEKDL-------------------PIVL--TVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECL-NYANLVSSKKCDFLNAEISTNTVTKSDPIK-CKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKW---QGICNHE--TGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKL-HNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDII-ISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILN----GA----------SESKKIYQNITLIKD--------KLFYRNEELRNINLKFIRQK----SKDMDNLINEMSK-NVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKP-ESEYNT--TIKLIDVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXX---XXLRNLK--IDTKVVVNKSNYFKLKSASNSKISE---RFQSLLNKSHNLNDKIKASHYKLKL----------NKKLIVDKELKLQSLSKDIDEKLSRL 5069
M L + + + L E +VC + C P GDLLIGR L A+STCG+ E +C+V S + KC +CDS+ Y + SH IENVV+ + + WWQS +G+ NV+I++ E+EF FT + FKT+RPAAM IERS D+G TW Y YF+ +C FPS+ + +++V+C + +S I PS G V+F + D Y + +N + ITN+RV+F +LHT GD +D I+ KYYYAI + ++RG C CYGHAS+C +G + E MV+G C C HNT G +CE+C+DFY+D PW PA+ +TN C+KC CN HS SCHFD VY+ S +SGGVCD+CQHNT NCE+CK ++++ I C C+CD +G+ +C ++ LGL +G C CK NVEG +CD+CK G + + + GC C C +GT+ CD+ +G CYCK VTG +C C ++ LSN +++GC C C ++G N C SG+C C+ +GF I D E K P H ++ +N+P + S Y+ +I + + + ++ ++ + V S C + D Q++ SRY+V+P C E+ L P L ++ + + L NS CL N +V + D I + + +K C+C+ GS + C+ GGQCQC PNV G+ C+RCA ++ F +GC+ C C GS++S C+ TG+C C G G+ CD C PGYW FP C C CN + CD +TG+CL+C+ ++ G+NC+ C GYYGD G C+ C CP G S + S C +++ C+C +GY G C++C ++GN G C+PC+C+ NID +SC+++TG C KCL++T G +C +C GY+GN ++C KCVCN MGT + G CN + +G+C CLPNVVG CD+C +N++S GC C+CD P + SCN++ GQC C+ G G+ C C++ FWG+P C C C+ G +QCN+ G C C G++G CD C +G G+ P C C C+ +WD + + N +L + + + + P + ++E + S++ I P + I E+ + L + NSN ++ + S I+ +N++ K K + + + +L++ ++ + N GA S++ +I N + I L EEL N + Q+ SK +DNL ++ ++ + TC + S S CGG+ C + R + C G + N + + L+ + + SK + +A VK E++ N + + T+ + S E L+ +I+ +L ADL+ E++ + + ++ ++ L ++IR R+ L +V +IL++S + + + + A +A+++ S + ++ + + N Q E A +KG + E+E + +S T R+ L+ + + + NT ++ + A LK +D+++ S +L + ++E R + L +++ NL ++AS KL+L N+KL+ DK +L L K + E L +
Sbjct: 1 MLLQLAALSILGAWALADVPELGDVCTEGSCYPATGDLLIGRAQQLLATSTCGVHKPEPFCIV--SHLQEEKKCFVCDSRQAYNETAHQVTSHSIENVVTTFAPNRLKTWWQSENGLENVTIQLDLEAEFHFTHLIMTFKTFRPAAMVIERSADFGNTWQVYRYFAYDCESSFPSVSHGPMTKVDDVICDTRYSDIEPSTEGEVIFRVLDPAFRIEDPYSPRIQN-MLKITNLRVKFTKLHTLGDNLLDSRIEIKEKYYYAIYDMVVRGNCFCYGHASECAPVDGTGEAVEGMVHGHCMCNHNTIGLNCERCQDFYHDLPWRPAEGRNTNACKKCHCNHHSHSCHFDMAVYRASGNVSGGVCDDCQHNTMGHNCEQCKPFFHQHPEKDIRDPNICEPCNCDPVGSLNGGVCDPMTDVSLGLISGQCRCKPNVEGERCDQCKQGHYGLS-EDPLGCQPCTCNALGTVPGGSPCDTDSGNCYCKRLVTGRNCDQCLPQHWGLSN-DMDGCRPCDC-DHGGAINNNCSPVSGQCQCREHMFGRRCDQVESGFYFIALDHYTYEAEEAKFGPGVTVVPRNHPQDRSPTWTGIGFVNVPEGAFLEFSIDNIPYSMEYDLIIRYEPQLPEQWEEVLMTVIRPRVITADSRCANTMPDDDNQMVSLHPGSRYVVLPRPVCFEEGLNYTVRLSLSLYSALSDVQSPYTLIDSIVLMPHCKNLDIFSGSGTEGGNLVTNSAWENFQRYRCLENSQAVVKTPMTDICRNYIFSVSALLHQGVKACQCDPQGSLSTVCDPSGGQCQCRPNVVGRNCDRCAPATFLFGPQGCRSCDCSPEGSVHSYCHEATGQCECIAGAYGRQCDRCLPGYWGFPNCRPCTCNGHAEQCDPQTGQCLSCRDHTTGHNCERCLGGYYGDPVLGSGDHCRPCMCPDGPGSGRQFSGACYKSPDSSQVFCVCNQGYKGARCEECAPGYYGNPHEVGGECRPCQCNSNIDMMDPESCDARTGACVKCLYHTEGESCNRCRLGYYGNALTQSCRKCVCNQMGTVEEMCPSSGNCNCDLTSGQCLCLPNVVGQHCDQCAPDTWNMASGKGCEDCDCD-PNHSFGSSCNEIMGQCSCKPGFGGRTCRECRELFWGNPEV--KCHACDCDPRGIAEQQCNKVTGHCVCVEGVSGPRCDTCARGYTGEFPQCERCHQCFAEWDIIVGDLTNQTHRLVQKVNTIKATGITGPYQA--TINNVENSANSIRNILAQNPATQPLTEIQGLLEQATALMAEM-NSN-LNLTEETLSEISSDNNSTDTKLKSLKEEAQKLEQTVKDLREQVEFVKNSDIRGARASVTRYYEQSQNAEIRANASTIDPYNLVNQSATLRTETEELMNQTKEEFNQRQDEFSKKLDNLAGQLETLDLSELSEKTCGSPAGSENCADSP-CGGLSCVDTQGSRKC-GGEGCDGLTTLAHNAW------QKAKDFDLEIISAMEEVDKLSKMVSEAKVKADEAKLNAQEVLAKTNETKKRVDSSNEELRQLIKQIRDFLTQDGADLESIEAVANEVLQMQMPTTPAQLQNLTEEIRERVGSLTDVEDILNQSAADILRAESLLEQARKARKEASDVKSTAEMV---NEALQHAERAQNSVAEALKQAAVDIKGTQDLLVSVESETSDSELKLSNATRRLLKLESDVALLKEKALNTSISANSTEKEAESINALAEQLKKDLDSELKDKYSTVEELITQKAEGVAEAKKRAEKLQDEARNL--LLQASE-KLQLLKNLEKNYDQNQKLLEDKANELVDLEKAVKELLQEI 1773
BLAST of Netrin-4 vs. Ensembl Zebrafish
Match: lamb1b (laminin, beta 1b [Source:ZFIN;Acc:ZDB-GENE-041210-197]) HSP 1 Score: 781.171 bits (2016), Expect = 0.000e+0 Identity = 505/1547 (32.64%), Postives = 768/1547 (49.64%), Query Frame = 3
Query: 84 IIFVARYILEIESD--NVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCL-------KCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYI-----------------------------EKPTYP------LEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLS-IYHVGKSS-CQSYQDTMQLI---KDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVL---------------GECLNYA-NLVSSKKCDFLNAEI-STNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSK--WQGI--CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAM---------------ISKNKIR--------DVTGAFNELKKSLKNILNG-----ASESKKIYQNITLIKD--KLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSI-ILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLI--DVTRGAIQSR-RIESV-----NLLIKIKTYLNGSADLKDFESLIENINKFEI 4379
I+ +A + L D ++C + C P GDLLIGR L ASSTCGL + E +C+V + + C CDS+ Y F+ SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + F+T+RPAAM IERS DYGRTW Y Y++ NC +P +P+ +++V+C S +S + PS G V+F + D Y +N + ITN+R+ +LH GD + R KY+YA+ + +IRG C CYGHASKC + V+ MV+G C C H+T G +CE+C+DFY D PW PA TN C+KCECN HS SCHFD VY S SGGVCD+C+HNT+ + CE CK YY++ I + C C CD G+ + +C + + AG C CK NVEG +CD CK G + + ++ GC C+C +GTL CD TG CYCK VTG +C C ++ LSN +++GC C C + G N C + +G+C CK G RCD+++S +Y KPT+ + + + N+ +S YN +I + ++ I+ L + +S+ C + D Q++ SRY+V+PN CLE L + + ++ S +V L+ + + + CL + +++ D I S + + + ++CKC+ GS + C+ IGGQCQC PNVSGK C+ CA +Y F GC+ C C+ GS N C+ TG+CLC G G+ C CKP +W +P C C CN S C TGKCLNC+G++ G+NC+ CE+GY+ T G C+ C CP G S S C NT C+C GY G C++C + ++GN G C PC+C+GNI+ + +SC+++TG C KCLF+T G C+ C GY+G+ + C +C+CN +G++ + G+ C+ +G+CPCLP V G CDRC +N++S GC PC C +P + + SC+ L+GQC C+ G GK C C++ FWGDP C C C+ G +QCN+ +G C C G+ GR+CD C +G G P+C +C C+ +WD + + N +L ++ M + D+ SL+ + L+EI + D + F+ +K Y ++++ + ++ STD+ +A+ I+ +K + D GA + ++ + + S+ + Q+ L KD R E N+ + Q+ ++ +N S ++ + C + L E CGG+GCK H +C G+ ++ R+ S+ K +Q+ ++ + + V KL DV A QS+ R+E NL+ +I+ +L D K E + + K +
Sbjct: 7 ILAIALHTLAQLPDHNDMCAEGSCYPATGDLLIGRAHRLTASSTCGLNSPEEFCMVNILED---TVCFECDSRRLYNEFTHPDSHTIENVVTTFAPNRLKTWWQSENGVENVTIQLDLEAEFHFTHLIMTFRTFRPAAMLIERSADYGRTWQVYRYYAFNCNESYPHVPKGPIKKVDDVICDSRYSGMEPSTEGEVIFRVLDPAFKIPDPYSPHIQN-LLKITNLRIAMEKLHKLGDLS-NYRKEARKKYFYAVYDMVIRGNCFCYGHASKCAPIGNEAGVEGMVHGHCMCNHHTTGLNCEECEDFYQDQPWRPAVGRETNACKKCECNRHSDSCHFDMAVYLSSGNRSGGVCDDCEHNTQGQWCENCKPFYYKHPQRDIRDPRICEPCDCDPRGSLYEGLCDSATNVMRDMIAGQCHCKANVEGQRCDHCKKGHYGLS-DDPLGCRLWLPRSSSCSCNPLGTLPGGTPCDIQTGSCYCKRLVTGRNCDQCLPQHWGLSN-DMDGCRPCDC-DVGGALNNNCSQETGQCACKEHMFGRRCDQLQSGYYFAALDHYIYEAEFASFGPAVKVVQRPLPQDRKPTWTGTGFVNVPEGETLTFNINNFPRSMEYNLMIRYEPLLPDEWEQVIVKLERPRDIDQSTHCTTTYDDEQMVSLHPGSRYVVVPNPVCLETGQNYTLKISFPQYSSYSYHSSPRTLVDSLVLLPEVQDLDLFCGGDHQQKNWEMFQKYRCLERSQSVIKIPLTDICKDYIFSVSALLHNGALECKCDPRGSLSTVCDPIGGQCQCRPNVSGKNCDACAPATYLFGPSGCRACDCNPLGSQNLFCHPSTGQCLCVPGAFGRQCGNCKPNHWGYPTCKPCSCNGNSEQCHPLTGKCLNCRGHTSGHNCERCESGYHRSTLLGSRDQCRPCMCPNGPGSGMQFSESCYQNPNTLQMVCVCNAGYKGPKCEECASGYYGNTRVPGGRCHPCQCNGNINMQDPESCDARTGACLKCLFHTDGTECQYCSRGYYGDARSQNCRRCMCNLVGSSRQSCVDGLCECDRISGQCPCLPGVEGQLCDRCAPNTWNINSGRGCQPCRC-HPEHSYSSSCDLLSGQCSCKPGFGGKTCEECRELFWGDPEV--ECHACDCDPRGIATQQCNKTSGDCVCIEGVAGRSCDTCGRGFVGTFPDCEACHHCFREWDVNVGELTNHTQRLVDTVEE-MKDSGVATPYKDIIKSLDDESRQLREI-----LEDDKVQQTFAHTQRMMKKARYTASLLRKSMDRSETDLDMVSTDHHRAIKDLKSLTKEAQNLQEITSDKQQQVLNIKHSDPRGAVDSIRDYYRESAEADFRVSQAISEPVRQSALLRKDAEAKLERTENEFNLKHQLYTQRLSKIETELN--STDLSRLSRQVCGG---AAGLDGCEDCGGLGCK------HEDGMSHCGGEG-------CDTIVTRSQDSLNKAKNLDQEVLHTLKEVDKLNRMVSDARGRADEAKLSAQDVLLRANQSKARVEQTNEDLRNLIQQIRDFLTNETDPKRIEEVANEVMKLSL 1518
BLAST of Netrin-4 vs. Ensembl Zebrafish
Match: lamb2l (laminin, beta 2-like [Source:ZFIN;Acc:ZDB-GENE-030131-4205]) HSP 1 Score: 780.4 bits (2014), Expect = 0.000e+0 Identity = 444/1299 (34.18%), Postives = 673/1299 (51.81%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQD---PFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMG-TLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEI------KINLPPVSKSGY-------------------------------------YNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLI-----KDSRYIVIPNK-CLEKDLPIV--------------LTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGE--CL-NYANLVSSKKCDFLNAEI-STNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTS--RGILCKKCQCPG-LSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT--NSKWQGI--CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKE-IKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNK-IRDVTGAFNELKKSLKNILNGA 3785
C C P G+LLIGR L SSTCGL E YC+V S KC ICDS+NPY SHR+ENV+ P D WWQ+++G NVS+ ++ E+EF FT ++FKT+RPAAM IERS D+GRTW Y YF+ NC + FP +P I+EV+C +S I PS G V++ D Y + D+ ITN+R+ F +LHT GD +D P++ KYYYA+ E ++RG C CYGHAS+C G + M++G C C+HNT G +CE+C+DFY+D+PW PA+ ++ + CR+C CN HS+ CHFD VY + +SGGVC+NC HNT +NCE CK YY++ I C+ C CD +G+ +C +LDLG+ G C CK+NV G +CD CK G + + ++ GC CNC G T CD +G C CK +VTG C+ C Y+ LS+ ++ GC C C ++G + C SG+C C+ G +C E++ ++ Y + + + LPP S + Y+ +I + ++ + +++ + V S+ C + T QL + RYI +P C E + V LT F + + + P ++ + + R+ + CL ++ + + + I S + + + + C+C+ GS + C+++GGQC+C PNV G+ C +CA +YGF GC C C GS+ C TG+C+CR G G+ C C+PG W FP C C+CN + +CD ++G C +C+ N+ G C+ C +GYYG+ + G C+ C CPG S +N C + + + C C +GY+G CD+C ++G+ G+C+PC+C+GNID + +SC+ +TG C KCL+NT G +C +C GY+GN ++ C +C C GT NS G+ C+ +TG+CPC N++G CD+C +N S GC PC C P AL+ CN +GQC+CQ G G+ CN C+ WGDP C +C C+ GS QC+R G C CK G +G+ CD C +G G P C C C+ WD + V+ + + + + P D LE+ + +E ++ G +IS + L R + + + ++ N +T +++ D + + + K ++D+ ++L++ L+N L
Sbjct: 16 CMDGSCYPATGNLLIGRAINLTTSSTCGLDGPEPYCIV--SHLKDSEKCFICDSENPYDANYHRDSHRVENVI--YPMDSTGELTWWQAVNGEENVSVRLNLEAEFHFTHLIMKFKTFRPAAMIIERSSDFGRTWRIYRYFAYNCTKTFPRVPAHSLRFIDEVICEERYSDIEPSTDGEVIYKVLDPAIHVKDPY-SLDIQDLLRITNLRINFTKLHTLGDNLLDRRPDVLQKYYYALYELVVRGSCFCYGHASECAPVPGVTTRDSGMIHGRCVCKHNTVGLNCERCRDFYHDAPWRPAETDNAHTCRECNCNGHSNQCHFDMAVYLATGNVSGGVCNNCLHNTMGRNCESCKPFYYQDPEKDIRDPAACVPCDCDPVGSVEGGVCDSHTDLDLGMIGGQCRCKQNVRGQRCDYCKEGHYGLSLDDPLGCQPCNCDPRGITTLGVPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSH-DLAGCRPCNC-DFGGATSSRCMMDSGQCDCRPHLIGRQCSEVQPAFFCASLDYYKYEAEAVMGRSPEDLTLPPSVSSVHRERQPGQMVTWTGPGFARVKDGAGLEFAISNIPYAMEYDIMIRYEPESTEDWEALVSVTSVNLPISLRCGNMLPTEQLYTVPMPHNVRYIKMPKPFCFEPNNRYVVSIRFQRHAVSHRHLTAFILIDSLVLIPRYEELTGFQGDEGADERRRQEMESYMCLESFMTMPMPALAELCTSLICSISAIMHNGALGCQCDPQGSLSAECDRVGGQCRCKPNVIGRKCNQCAPGTYGFGPYGCTVCDCHSQGSVGHHCDPVTGQCMCRHGAIGRQCSECQPGQWGFPNCRQCQCNSHAESCDPESGACYDCRDNTAGQLCERCVDGYYGNPALGSGEHCRPCPCPGHPDSGHSNGASCEVDYASNQILCQCGQGYAGPRCDRCAPGYYGDPEHPGGSCRPCQCNGNIDPQDPESCDPRTGLCLKCLYNTDGDSCSECKLGYYGNALIQDCRRCTCVTAGTLPNSCTDGVCQCDKQTGQCPCKQNIIGHNCDQCATNHWNFGSDCGCEPCGCVQP-NALSTHCNMFSGQCHCQPGFGGRQCNECEAFHWGDPRVQ--CEECNCHPLGSETSQCDRTTGACVCKDGASGQRCDECARGFTGNFPKCVPCHPCFQLWDDVVCQVRRDLTHFRDIIAMILEKGEVPGVSDKRILELEKKLAQAQELVRDGDREGTFNLISQTVDDL-RAEIALTDGRLMGVIRDLN--VTTDHNNDLKQNLTALEKELKDLNNTLHQLRRELENYLTAG 1301
BLAST of Netrin-4 vs. Ensembl Zebrafish
Match: lamb1b (laminin, beta 1b [Source:ZFIN;Acc:ZDB-GENE-041210-197]) HSP 1 Score: 772.696 bits (1994), Expect = 0.000e+0 Identity = 461/1315 (35.06%), Postives = 686/1315 (52.17%), Query Frame = 3
Query: 78 VFIIFVARYILEIESD--NVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYI-----------------------------EKPTYP------LEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLS-IYHVGKSS-CQSYQDTMQLI---KDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVL---------------GECLNYA-NLVSSKKCDFLNAEI-STNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSK--WQGI--CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNI 3815
+I +A + L D ++C + C P GDLLIGR L ASSTCGL + E +C+V + + C CDS+ Y F+ SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + F+T+RPAAM IERS DYGRTW Y Y++ NC +P +P+ +++V+C S +S + PS G V+F + D Y +N + ITN+R+ +LH GD + R KY+YA+ + +IRG C CYGHASKC + V+ MV+G C C H+T G +CE+C+DFY D PW PA TN C+KCECN HS SCHFD VY S SGGVCD+C+HNT+ + CE CK YY++ I + C C CD G+ + +C + + AG C CK NVEG +CD CK G + + ++ GC C+C +GTL CD TG CYCK VTG +C C ++ LSN +++GC C C + G N C + +G+C CK G RCD+++S +Y KPT+ + + + N+ +S YN +I + ++ I+ L + +S+ C + D Q++ SRY+V+PN CLE L + + ++ S +V L+ + + + CL + +++ D I S + + + ++CKC+ GS + C+ IGGQCQC PNVSGK C+ CA +Y F GC+ C C+ GS N C+ G+CLC G G+ C CKP +W +P C C CN S C TGKCLNC+G++ G+NC+ CE+GY+ T G C+ C CP G S S C NT C+C GY G C++C + ++GN G C PC+C+GNI+ + +SC+++TG C KCLF+T G C+ C GY+G+ + C +C+CN +G++ + G+ C+ +G+CPCLP V G CDRC +N++S GC PC C +P + + SC+ L+GQC C+ G GK C C++ FWGDP C C C+ G +QCN+ +G C C G+ GR CD C +G G P+C +C C+ +WD + + N +L ++ M + D+ SL+ + L+EI + D + F+ +K ++++ + ++ STD+ +A I+D+ L K +N+ S+ ++ NI
Sbjct: 8 ALVISIALHTLAQLPDHNDMCAEGSCYPATGDLLIGRAHRLTASSTCGLNSPEEFCMVNILED---TVCFECDSRRLYNEFTHPDSHTIENVVTTFAPNRLKTWWQSENGVENVTIQLDLEAEFHFTHLIMTFRTFRPAAMLIERSADYGRTWQVYRYYAFNCDESYPHVPKGPIKKVDDVICDSRYSGMEPSTEGEVIFRVLDPAFKIPDPYSPHIQN-LLKITNLRIAMEKLHKLGDLS-NYRKEARKKYFYAVYDMVIRGNCFCYGHASKCAPIGNEAGVEGMVHGHCMCNHHTTGLNCEECEDFYQDQPWRPAVGRETNACKKCECNRHSDSCHFDMAVYLSSGNRSGGVCDDCEHNTQGQWCENCKPFYYKHPQRDIRDPRICEPCDCDPRGSLYEGLCDSATNVMRDMIAGQCHCKANVEGQRCDHCKKGHYGLS-DDPLGCRSCSCNPLGTLPGGTPCDIQTGSCYCKRLVTGRNCDQCLPQHWGLSN-DMDGCRPCDC-DVGGALNNNCSQETGQCACKEHMFGRRCDQLQSGYYFAALDHYIYEAEFASFGPAVKVVQRPLPQDRKPTWTGTGFVNVPEGETLTFNINNFPRSMEYNLMIRYEPLLPDEWEQVIVKLERPRDIDQSTHCTTTYDDEQMVSLHPGSRYVVVPNPVCLETGQNYTLKISFPQYSSYNYHSSPRTLVDSLVLLPEVQDLDLFCGGDHQQKNWEMFQKYRCLERSQSVIKIPLTDICKDYIFSVSALLHNGALECKCDPRGSLSTVCDPIGGQCQCRPNVSGKNCDACAPATYLFGPSGCRACDCNPLGSQNLFCHPSNGQCLCVPGAFGRQCGNCKPNHWGYPTCKPCSCNGNSEQCHPLTGKCLNCRGHTSGHNCERCESGYHRSTLLGSRDQCRPCMCPNGPGSGMQFSESCYQNPNTLQMVCVCNAGYKGPKCEECASGYYGNTRVPGGRCHPCQCNGNINMQDPESCDARTGACLKCLFHTDGTECQYCSRGYYGDARSQNCRRCMCNLVGSSRQSCVDGLCECDRISGQCPCLPGVEGQLCDRCAPNTWNINSGRGCQPCRC-HPEHSYSSSCDLLSGQCSCKPGFGGKTCEECRELFWGDPEV--ECHACDCDPRGIATQQCNKTSGDCVCIEGVAGRRCDTCGRGFVGTFPDCEACHYCFREWDVNVGELTNHTQRLVDTVEE-MKDSGVATPYKDIIKSLDDESRQLREI-----LEDDKVQQTFAHTQRMMKKASETASLLRKSMDRSETDLDMVSTDHHRA------IKDL----KSLTKEAQNLQEITSDKQQQVLNI 1295
BLAST of Netrin-4 vs. Ensembl Xenopus
Match: LAMB1 (laminin subunit beta 1 [Source:NCBI gene;Acc:100495516]) HSP 1 Score: 880.937 bits (2275), Expect = 0.000e+0 Identity = 567/1785 (31.76%), Postives = 881/1785 (49.36%), Query Frame = 3
Query: 63 MTLNYVFIIFV-ARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQ-VKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTY----------------PLEHLKE---IKINLPPVSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESN----------------NKXXXXXXXXXXXXXXXXNSTKTRKVLGECL-NYANLVSSKKCDFL-NAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT-----NSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHN-SSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDII-ISNFSEKLSRLKYYVYNS--------NIIDFNTQQNSIITIENSTDYAKAM------ISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNI---NLKFIRQKSKDMDNLINEMSKN--VITFNGVTCNTITESNPLMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLI---DVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNK-SNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKL--IVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
+T FI+ + A Y + + C + C P GDLLIGR L ASSTCG+ E +C+V S + KC ICDS +PY ++T SHRIENVV+ P WWQS +G+ NV+I++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FP I +++++C S +S I PS G V++ + +D Y + ++ + ITN+R++F++LHT GD +D I+ KYYYAI + ++RG C CYGHAS+C +G V+ MV+G C C+HNTKG +CE+C DFY+D PW PA+ S+N C+KC CN HS+ CHFD VY + SGGVCD+CQHNT +NCE+CK +Y++ I C C+CD G++ IC + + +GL AG C CK NVEG +CD+CK GF+ N + GC C C +GT+ CDS G C+CK FVTG C C ++ LSN +++GC C C GS DN C SG+C C+ G +C+E++S +Y ++ TY PL H + I V + Y F I +M+ +D I V+++ GK S ++ L SRYI +P C EK L + L +++ SN K + N+ CL N ++V + D N + + + ++C+C+ GS ++ C+ GGQCQC PNV G+ C +CA ++GF GCK C+CD+ GS + C+ +TG+C C G G+ CD C PGYW FP C C+CN + C++ TG+C NCK +S GY+C+ C +GYYGD G C+ C CP G S + + GC T C C GY+G CD C + +FGN G CQPC C+ NID +C+ ++G C KCL++T G NC++C GY+GN + C +CVCN++GT + + CN G+CPCLPNV+G CDRC +NL+S GC PC CD +L SCN+ TGQC C G G+ C+ C++ FWG+P C C C+ G QCNR G C CK G+ G CD C +G G P C+ C C+ WD + + N +L N ++ + +S V P + +++ ++ ++ I + P + I N E+ +LK V I N + ++ + KA+ + KI +V GA + + K + L N I + +E+ N+ NL +++ ++ L++E++ N + + ++ T S L+C + CGG+ CK D R C G + + + T + L E + SK + ++ ++ E + ++ + T+ + + NL+ +I+ +L ADL E++ + K ++ ++ L ++IR R+ L+ V+ IL +S + + + A + + + + ++ + K + ++ + A +KG + E+EA + ++ T+R+ L+ I + K + Q ++ N KV+ + +N +K +K +E + K+ L + K + NKKL I D E + + ++EK +L+ L++ + +
Sbjct: 3 VTFRLTFILMLGAAYAQSPDFSHGCAEGSCYPATGDLLIGRSDQLTASSTCGIQKPESFCIV--SHLQEEKKCFICDSLDPYNEQNNTESHRIENVVTTFAPNRLNLWWQSENGLENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWKIYRYFAYDCETSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIYRVLDPAFRIDDPYSLRIKS-MLRITNLRIKFLKLHTLGDNLLDSRVEIKEKYYYAIYDMVVRGNCFCYGHASECAPVKGYSDNVEGMVHGRCVCRHNTKGLNCEQCLDFYHDLPWRPAEGRSSNACKKCNCNEHSTHCHFDMAVYMSTGNTSGGVCDDCQHNTMGRNCEQCKPFFYQHPDKDIRDPNICQPCNCDPAGSQDGGICDRYSDFSVGLIAGQCRCKLNVEGERCDQCKEGFYDLNAEDPEGCQFCGCNPLGTIPGGNSCDSRNGNCFCKRFVTGRKCDQCAPEFWGLSN-DLDGCRPCDCDHGGSEDN-NCSPESGQCRCRPHMIGRQCNEVESGFYFISLDHYTYEAEYANFGPDVRIVERPLPHDRTPSWTGIGFARVPEGAYLEFHINDIPTSME-YDLLIRYEPQLPEQWEEAVITVIRPGKIPTSSRCGNTVPSDDNQSVALPPGSRYITLPRPICFEKGLNYTIRLELPRYSVNSNVENPYTLIDSIVLLPHVKSLDIFSMGGSGDDVTTNTAWQTFQRYRCLENSQSVVKTPLTDVCKNFLFTISALLHKSALECQCDPQGSLSSVCDPNGGQCQCRPNVIGRNCNKCAPGTFGFGPNGCKPCECDVRGSSHPFCHTETGQCPCIHGAFGRQCDVCLPGYWGFPSCRQCQCNGHAEDCNALTGECTNCKDHSEGYSCERCLSGYYGDPRLGSGDHCRPCPCPDGPGSERQFASGCYKDPTTLQVVCSCNMGYTGIRCDGCASGYFGNPEEIGGVCQPCHCNNNIDMSDSLACHKQSGLCLKCLYHTEGINCQECQLGYYGNAIQQDCRRCVCNYLGTEQENCSGEIDCHCNRTNGQCPCLPNVIGQSCDRCAANTWNLASGTGCEPCACDTN-RSLGLSCNEFTGQCQCMPGFGGRDCSECQELFWGNPDI--ECRACDCDSRGIQTSQCNRATGHCICKEGVEGVRCDKCARGYSGAFPYCTPCHKCFALWDIIIQELSNRTKQLLNRANDLKISGVIGPYQ--QTVDKVQEKLEEIRSILAHNPAAQPLKNIENLFEEAEKLKSEVSQKVDEVEMKLAEIKINDTEKLDALQADAESFGKALKELGDQLEFMKISNVQGAMDSINKYFQMSLEAEEMVNASVSNPDSIIEHSAATRQEVENLINENLSQFKKEQEEQTRLLDELAGNGQSLDLSSLSEKTCGNSPGLLCEDSECGGLNCKTDDGKRKC-GGPGCGGLVTVAHSAWKTASSFDTEIQNALAEVEEL------SKMVAESKLRAEEAKQSAQDVLTKANATKEKVNQSNEDLRNLIKQIREFLMQDGADLDSIEAVASEVLKMDMPTTPEQLQKLTEEIRERVESLSGVDTILKQSAGDIARAEALLDEAKESSKSAADIKATVDAVKEALMEAGKAQSAAEKAI---KRADDDIKGTNDLLTSIESEAAVSEETLNNATKRLVQLENNIDDLMKKSAANSQTANQVEKSVHEVKANANGAKKVLDGELTNKYKTVEDLIAKKAEDSEEARRKAQELQKEAKG--LLEQANKKLDTIKDLEKTYEGNERILEEKAKQLVQLEETVRA 1764
BLAST of Netrin-4 vs. Ensembl Xenopus
Match: LAMB1 (laminin subunit beta 1 [Source:NCBI gene;Acc:100495516]) HSP 1 Score: 880.937 bits (2275), Expect = 0.000e+0 Identity = 566/1787 (31.67%), Postives = 881/1787 (49.30%), Query Frame = 3
Query: 54 LI*MTLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQ-VKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTY----------------PLEHLKE---IKINLPPVSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESN----------------NKXXXXXXXXXXXXXXXXNSTKTRKVLGECL-NYANLVSSKKCDFL-NAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT-----NSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHN-SSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDII-ISNFSEKLSRLKYYVYNS--------NIIDFNTQQNSIITIENSTDYAKAM------ISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNI---NLKFIRQKSKDMDNLINEMSKN--VITFNGVTCNTITESNPLMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLI---DVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNK-SNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKL--IVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
L M ++ ++ A Y + + C + C P GDLLIGR L ASSTCG+ E +C+V S + KC ICDS +PY ++T SHRIENVV+ P WWQS +G+ NV+I++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FP I +++++C S +S I PS G V++ + +D Y + ++ + ITN+R++F++LHT GD +D I+ KYYYAI + ++RG C CYGHAS+C +G V+ MV+G C C+HNTKG +CE+C DFY+D PW PA+ S+N C+KC CN HS+ CHFD VY + SGGVCD+CQHNT +NCE+CK +Y++ I C C+CD G++ IC + + +GL AG C CK NVEG +CD+CK GF+ N + GC C C +GT+ CDS G C+CK FVTG C C ++ LSN +++GC C C GS DN C SG+C C+ G +C+E++S +Y ++ TY PL H + I V + Y F I +M+ +D I V+++ GK S ++ L SRYI +P C EK L + L +++ SN K + N+ CL N ++V + D N + + + ++C+C+ GS ++ C+ GGQCQC PNV G+ C +CA ++GF GCK C+CD+ GS + C+ +TG+C C G G+ CD C PGYW FP C C+CN + C++ TG+C NCK +S GY+C+ C +GYYGD G C+ C CP G S + + GC T C C GY+G CD C + +FGN G CQPC C+ NID +C+ ++G C KCL++T G NC++C GY+GN + C +CVCN++GT + + CN G+CPCLPNV+G CDRC +NL+S GC PC CD +L SCN+ TGQC C G G+ C+ C++ FWG+P C C C+ G QCNR G C CK G+ G CD C +G G P C+ C C+ WD + + N +L N ++ + +S V P + +++ ++ ++ I + P + I N E+ +LK V I N + ++ + KA+ + KI +V GA + + K + L N I + +E+ N+ NL +++ ++ L++E++ N + + ++ T S L+C + CGG+ CK D R C G + + + T + L E + SK + ++ ++ E + ++ + T+ + + NL+ +I+ +L ADL E++ + K ++ ++ L ++IR R+ L+ V+ IL +S + + + A + + + + ++ + K + ++ + A +KG + E+EA + ++ T+R+ L+ I + K + Q ++ N KV+ + +N +K +K +E + K+ L + K + NKKL I D E + + ++EK +L+ L++ + +
Sbjct: 3 LFQMPGYFLVLMLGAAYAQSPDFSHGCAEGSCYPATGDLLIGRSDQLTASSTCGIQKPESFCIV--SHLQEEKKCFICDSLDPYNEQNNTESHRIENVVTTFAPNRLNLWWQSENGLENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWKIYRYFAYDCETSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIYRVLDPAFRIDDPYSLRIKS-MLRITNLRIKFLKLHTLGDNLLDSRVEIKEKYYYAIYDMVVRGNCFCYGHASECAPVKGYSDNVEGMVHGRCVCRHNTKGLNCEQCLDFYHDLPWRPAEGRSSNACKKCNCNEHSTHCHFDMAVYMSTGNTSGGVCDDCQHNTMGRNCEQCKPFFYQHPDKDIRDPNICQPCNCDPAGSQDGGICDRYSDFSVGLIAGQCRCKLNVEGERCDQCKEGFYDLNAEDPEGCQFCGCNPLGTIPGGNSCDSRNGNCFCKRFVTGRKCDQCAPEFWGLSN-DLDGCRPCDCDHGGSEDN-NCSPESGQCRCRPHMIGRQCNEVESGFYFISLDHYTYEAEYANFGPDVRIVERPLPHDRTPSWTGIGFARVPEGAYLEFHINDIPTSME-YDLLIRYEPQLPEQWEEAVITVIRPGKIPTSSRCGNTVPSDDNQSVALPPGSRYITLPRPICFEKGLNYTIRLELPRYSVNSNVENPYTLIDSIVLLPHVKSLDIFSMGGSGDDVTTNTAWQTFQRYRCLENSQSVVKTPLTDVCKNFLFTISALLHKSALECQCDPQGSLSSVCDPNGGQCQCRPNVIGRNCNKCAPGTFGFGPNGCKPCECDVRGSSHPFCHTETGQCPCIHGAFGRQCDVCLPGYWGFPSCRQCQCNGHAEDCNALTGECTNCKDHSEGYSCERCLSGYYGDPRLGSGDHCRPCPCPDGPGSERQFASGCYKDPTTLQVVCSCNMGYTGIRCDGCASGYFGNPEEIGGVCQPCHCNNNIDMSDSLACHKQSGLCLKCLYHTEGINCQECQLGYYGNAIQQDCRRCVCNYLGTEQENCSGEIDCHCNRTNGQCPCLPNVIGQSCDRCAANTWNLASGTGCEPCACDTN-RSLGLSCNEFTGQCQCMPGFGGRDCSECQELFWGNPDI--ECRACDCDSRGIQTSQCNRATGHCICKEGVEGVRCDKCARGYSGAFPYCTPCHKCFALWDIIIQELSNRTKQLLNRANDLKISGVIGPYQ--QTVDKVQEKLEEIRSILAHNPAAQPLKNIENLFEEAEKLKSEVSQKVDEVEMKLAEIKINDTEKLDALQADAESFGKALKELGDQLEFMKISNVQGAMDSINKYFQMSLEAEEMVNASVSNPDSIIEHSAATRQEVENLINENLSQFKKEQEEQTRLLDELAGNGQSLDLSSLSEKTCGNSPGLLCEDSECGGLNCKTDDGKRKC-GGPGCGGLVTVAHSAWKTASSFDTEIQNALAEVEEL------SKMVAESKLRAEEAKQSAQDVLTKANATKEKVNQSNEDLRNLIKQIREFLMQDGADLDSIEAVASEVLKMDMPTTPEQLQKLTEEIRERVESLSGVDTILKQSAGDIARAEALLDEAKESSKSAADIKATVDAVKEALMEAGKAQSAAEKAI---KRADDDIKGTNDLLTSIESEAAVSEETLNNATKRLVQLENNIDDLMKKSAANSQTANQVEKSVHEVKANANGAKKVLDGELTNKYKTVEDLIAKKAEDSEEARRKAQELQKEAKG--LLEQANKKLDTIKDLEKTYEGNERILEEKAKQLVQLEETVRA 1766
BLAST of Netrin-4 vs. Ensembl Xenopus
Match: LAMB1 (laminin subunit beta 1 [Source:NCBI gene;Acc:100495516]) HSP 1 Score: 878.626 bits (2269), Expect = 0.000e+0 Identity = 564/1779 (31.70%), Postives = 877/1779 (49.30%), Query Frame = 3
Query: 78 VFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQ-VKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTY----------------PLEHLKE---IKINLPPVSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESN----------------NKXXXXXXXXXXXXXXXXNSTKTRKVLGECL-NYANLVSSKKCDFL-NAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT-----NSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHN-SSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDII-ISNFSEKLSRLKYYVYNS--------NIIDFNTQQNSIITIENSTDYAKAM------ISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNI---NLKFIRQKSKDMDNLINEMSKN--VITFNGVTCNTITESNPLMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLI---DVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNK-SNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKL--IVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
++ A Y + + C + C P GDLLIGR L ASSTCG+ E +C+V S + KC ICDS +PY ++T SHRIENVV+ P WWQS +G+ NV+I++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FP I +++++C S +S I PS G V++ + +D Y + ++ + ITN+R++F++LHT GD +D I+ KYYYAI + ++RG C CYGHAS+C +G V+ MV+G C C+HNTKG +CE+C DFY+D PW PA+ S+N C+KC CN HS+ CHFD VY + SGGVCD+CQHNT +NCE+CK +Y++ I C C+CD G++ IC + + +GL AG C CK NVEG +CD+CK GF+ N + GC C C +GT+ CDS G C+CK FVTG C C ++ LSN +++GC C C GS DN C SG+C C+ G +C+E++S +Y ++ TY PL H + I V + Y F I +M+ +D I V+++ GK S ++ L SRYI +P C EK L + L +++ SN K + N+ CL N ++V + D N + + + ++C+C+ GS ++ C+ GGQCQC PNV G+ C +CA ++GF GCK C+CD+ GS + C+ +TG+C C G G+ CD C PGYW FP C C+CN + C++ TG+C NCK +S GY+C+ C +GYYGD G C+ C CP G S + + GC T C C GY+G CD C + +FGN G CQPC C+ NID +C+ ++G C KCL++T G NC++C GY+GN + C +CVCN++GT + + CN G+CPCLPNV+G CDRC +NL+S GC PC CD +L SCN+ TGQC C G G+ C+ C++ FWG+P C C C+ G QCNR G C CK G+ G CD C +G G P C+ C C+ WD + + N +L N ++ + +S V P + +++ ++ ++ I + P + I N E+ +LK V I N + ++ + KA+ + KI +V GA + + K + L N I + +E+ N+ NL +++ ++ L++E++ N + + ++ T S L+C + CGG+ CK D R C G + + + T + L E + SK + ++ ++ E + ++ + T+ + + NL+ +I+ +L ADL E++ + K ++ ++ L ++IR R+ L+ V+ IL +S + + + A + + + + ++ + K + ++ + A +KG + E+EA + ++ T+R+ L+ I + K + Q ++ N KV+ + +N +K +K +E + K+ L + K + NKKL I D E + + ++EK +L+ L++ + +
Sbjct: 2 TILMLGAAYAQSPDFSHGCAEGSCYPATGDLLIGRSDQLTASSTCGIQKPESFCIV--SHLQEEKKCFICDSLDPYNEQNNTESHRIENVVTTFAPNRLNLWWQSENGLENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWKIYRYFAYDCETSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIYRVLDPAFRIDDPYSLRIKS-MLRITNLRIKFLKLHTLGDNLLDSRVEIKEKYYYAIYDMVVRGNCFCYGHASECAPVKGYSDNVEGMVHGRCVCRHNTKGLNCEQCLDFYHDLPWRPAEGRSSNACKKCNCNEHSTHCHFDMAVYMSTGNTSGGVCDDCQHNTMGRNCEQCKPFFYQHPDKDIRDPNICQPCNCDPAGSQDGGICDRYSDFSVGLIAGQCRCKLNVEGERCDQCKEGFYDLNAEDPEGCQFCGCNPLGTIPGGNSCDSRNGNCFCKRFVTGRKCDQCAPEFWGLSN-DLDGCRPCDCDHGGSEDN-NCSPESGQCRCRPHMIGRQCNEVESGFYFISLDHYTYEAEYANFGPDVRIVERPLPHDRTPSWTGIGFARVPEGAYLEFHINDIPTSME-YDLLIRYEPQLPEQWEEAVITVIRPGKIPTSSRCGNTVPSDDNQSVALPPGSRYITLPRPICFEKGLNYTIRLELPRYSVNSNVENPYTLIDSIVLLPHVKSLDIFSMGGSGDDVTTNTAWQTFQRYRCLENSQSVVKTPLTDVCKNFLFTISALLHKSALECQCDPQGSLSSVCDPNGGQCQCRPNVIGRNCNKCAPGTFGFGPNGCKPCECDVRGSSHPFCHTETGQCPCIHGAFGRQCDVCLPGYWGFPSCRQCQCNGHAEDCNALTGECTNCKDHSEGYSCERCLSGYYGDPRLGSGDHCRPCPCPDGPGSERQFASGCYKDPTTLQVVCSCNMGYTGIRCDGCASGYFGNPEEIGGVCQPCHCNNNIDMSDSLACHKQSGLCLKCLYHTEGINCQECQLGYYGNAIQQDCRRCVCNYLGTEQENCSGEIDCHCNRTNGQCPCLPNVIGQSCDRCAANTWNLASGTGCEPCACDTN-RSLGLSCNEFTGQCQCMPGFGGRDCSECQELFWGNPDI--ECRACDCDSRGIQTSQCNRATGHCICKEGVEGVRCDKCARGYSGAFPYCTPCHKCFALWDIIIQELSNRTKQLLNRANDLKISGVIGPYQ--QTVDKVQEKLEEIRSILAHNPAAQPLKNIENLFEEAEKLKSEVSQKVDEVEMKLAEIKINDTEKLDALQADAESFGKALKELGDQLEFMKISNVQGAMDSINKYFQMSLEAEEMVNASVSNPDSIIEHSAATRQEVENLINENLSQFKKEQEEQTRLLDELAGNGQSLDLSSLSEKTCGNSPGLLCEDSECGGLNCKTDDGKRKC-GGPGCGGLVTVAHSAWKTASSFDTEIQNALAEVEEL------SKMVAESKLRAEEAKQSAQDVLTKANATKEKVNQSNEDLRNLIKQIREFLMQDGADLDSIEAVASEVLKMDMPTTPEQLQKLTEEIRERVESLSGVDTILKQSAGDIARAEALLDEAKESSKSAADIKATVDAVKEALMEAGKAQSAAEKAI---KRADDDIKGTNDLLTSIESEAAVSEETLNNATKRLVQLENNIDDLMKKSAANSQTANQVEKSVHEVKANANGAKKVLDGELTNKYKTVEDLIAKKAEDSEEARRKAQELQKEAKG--LLEQANKKLDTIKDLEKTYEGNERILEEKAKQLVQLEETVRA 1757
BLAST of Netrin-4 vs. Ensembl Xenopus
Match: LAMB1 (laminin subunit beta 1 [Source:NCBI gene;Acc:100495516]) HSP 1 Score: 877.47 bits (2266), Expect = 0.000e+0 Identity = 566/1781 (31.78%), Postives = 882/1781 (49.52%), Query Frame = 3
Query: 78 VFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQ-VKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTY----------------PLEHLKE---IKINLPPVSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESN----------------NKXXXXXXXXXXXXXXXXNSTKTRKVLGECL-NYANLVSSKKCDFL-NAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT-----NSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHN-SSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDII-ISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITI----------ENSTDYAKAM------ISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNI---NLKFIRQKSKDMDNLINEMSKN--VITFNGVTCNTITESNPLMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLI---DVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNK-SNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKL--IVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
+ ++ A Y + + C + C P GDLLIGR L ASSTCG+ E +C+V S + KC ICDS +PY ++T SHRIENVV+ P WWQS +G+ NV+I++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FP I +++++C S +S I PS G V++ + +D Y + ++ + ITN+R++F++LHT GD +D I+ KYYYAI + ++RG C CYGHAS+C +G V+ MV+G C C+HNTKG +CE+C DFY+D PW PA+ S+N C+KC CN HS+ CHFD VY + SGGVCD+CQHNT +NCE+CK +Y++ I C C+CD G++ IC + + +GL AG C CK NVEG +CD+CK GF+ N + GC C C +GT+ CDS G C+CK FVTG C C ++ LSN +++GC C C GS DN C SG+C C+ G +C+E++S +Y ++ TY PL H + I V + Y F I +M+ +D I V+++ GK S ++ L SRYI +P C EK L + L +++ SN K + N+ CL N ++V + D N + + + ++C+C+ GS ++ C+ GGQCQC PNV G+ C +CA ++GF GCK C+CD+ GS + C+ +TG+C C G G+ CD C PGYW FP C C+CN + C++ TG+C NCK +S GY+C+ C +GYYGD G C+ C CP G S + + GC T C C GY+G CD C + +FGN G CQPC C+ NID +C+ ++G C KCL++T G NC++C GY+GN + C +CVCN++GT + + CN G+CPCLPNV+G CDRC +NL+S GC PC CD +L SCN+ TGQC C G G+ C+ C++ FWG+P C C C+ G QCNR G C CK G+ G CD C +G G P C+ C C+ WD + + N +L N ++ + +S V P + +++ ++ ++ I + P + I N E+ +LK V S +D + + I I ++ + KA+ + KI +V GA + + K + L N I + +E+ N+ NL +++ ++ L++E++ N + + ++ T S L+C + CGG+ CK D R C G + + + T + L E + SK + ++ ++ E + ++ + T+ + + NL+ +I+ +L ADL E++ + K ++ ++ L ++IR R+ L+ V+ IL +S + + + A + + + + ++ + K + ++ + A +KG + E+EA + ++ T+R+ L+ I + K + Q ++ N KV+ + +N +K +K +E + K+ L + K + NKKL I D E + + ++EK +L+ L++ + +
Sbjct: 37 LVLMLGAAYAQSPDFSHGCAEGSCYPATGDLLIGRSDQLTASSTCGIQKPESFCIV--SHLQEEKKCFICDSLDPYNEQNNTESHRIENVVTTFAPNRLNLWWQSENGLENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWKIYRYFAYDCETSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIYRVLDPAFRIDDPYSLRIKS-MLRITNLRIKFLKLHTLGDNLLDSRVEIKEKYYYAIYDMVVRGNCFCYGHASECAPVKGYSDNVEGMVHGRCVCRHNTKGLNCEQCLDFYHDLPWRPAEGRSSNACKKCNCNEHSTHCHFDMAVYMSTGNTSGGVCDDCQHNTMGRNCEQCKPFFYQHPDKDIRDPNICQPCNCDPAGSQDGGICDRYSDFSVGLIAGQCRCKLNVEGERCDQCKEGFYDLNAEDPEGCQFCGCNPLGTIPGGNSCDSRNGNCFCKRFVTGRKCDQCAPEFWGLSN-DLDGCRPCDCDHGGSEDN-NCSPESGQCRCRPHMIGRQCNEVESGFYFISLDHYTYEAEYANFGPDVRIVERPLPHDRTPSWTGIGFARVPEGAYLEFHINDIPTSME-YDLLIRYEPQLPEQWEEAVITVIRPGKIPTSSRCGNTVPSDDNQSVALPPGSRYITLPRPICFEKGLNYTIRLELPRYSVNSNVENPYTLIDSIVLLPHVKSLDIFSMGGSGDDVTTNTAWQTFQRYRCLENSQSVVKTPLTDVCKNFLFTISALLHKSALECQCDPQGSLSSVCDPNGGQCQCRPNVIGRNCNKCAPGTFGFGPNGCKPCECDVRGSSHPFCHTETGQCPCIHGAFGRQCDVCLPGYWGFPSCRQCQCNGHAEDCNALTGECTNCKDHSEGYSCERCLSGYYGDPRLGSGDHCRPCPCPDGPGSERQFASGCYKDPTTLQVVCSCNMGYTGIRCDGCASGYFGNPEEIGGVCQPCHCNNNIDMSDSLACHKQSGLCLKCLYHTEGINCQECQLGYYGNAIQQDCRRCVCNYLGTEQENCSGEIDCHCNRTNGQCPCLPNVIGQSCDRCAANTWNLASGTGCEPCACDTN-RSLGLSCNEFTGQCQCMPGFGGRDCSECQELFWGNPDI--ECRACDCDSRGIQTSQCNRATGHCICKEGVEGVRCDKCARGYSGAFPYCTPCHKCFALWDIIIQELSNRTKQLLNRANDLKISGVIGPYQ--QTVDKVQEKLEEIRSILAHNPAAQPLKNIENLFEEAEKLKSEV--SQKVDEVEMKLAEIKINDTEKLDALQADAESFGKALKELGDQLEFMKISNVQGAMDSINKYFQMSLEAEEMVNASVSNPDSIIEHSAATRQEVENLINENLSQFKKEQEEQTRLLDELAGNGQSLDLSSLSEKTCGNSPGLLCEDSECGGLNCKTDDGKRKC-GGPGCGGLVTVAHSAWKTASSFDTEIQNALAEVEEL------SKMVAESKLRAEEAKQSAQDVLTKANATKEKVNQSNEDLRNLIKQIREFLMQDGADLDSIEAVASEVLKMDMPTTPEQLQKLTEEIRERVESLSGVDTILKQSAGDIARAEALLDEAKESSKSAADIKATVDAVKEALMEAGKAQSAAEKAI---KRADDDIKGTNDLLTSIESEAAVSEETLNNATKRLVQLENNIDDLMKKSAANSQTANQVEKSVHEVKANANGAKKVLDGELTNKYKTVEDLIAKKAEDSEEARRKAQELQKEAKG--LLEQANKKLDTIKDLEKTYEGNERILEEKAKQLVQLEETVRA 1792
BLAST of Netrin-4 vs. Ensembl Xenopus
Match: LAMB1 (laminin subunit beta 1 [Source:NCBI gene;Acc:100495516]) HSP 1 Score: 869.766 bits (2246), Expect = 0.000e+0 Identity = 558/1752 (31.85%), Postives = 868/1752 (49.54%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQ-VKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTY----------------PLEHLKE---IKINLPPVSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESN----------------NKXXXXXXXXXXXXXXXXNSTKTRKVLGECL-NYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT-----NSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHN-SSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDII-ISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAM------ISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNI---NLKFIRQKSKDMDNLINEMSKN--VITFNGVTCNTITESNPLMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLI---DVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNK-SNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKL--IVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C P GDLLIGR L ASSTCG+ E +C+V S + KC ICDS +PY ++T SHRIENVV+ P WWQS +G+ NV+I++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FP I +++++C S +S I PS G V++ + +D Y + ++ + ITN+R++F++LHT GD +D I+ KYYYAI + ++RG C CYGHAS+C +G V+ MV+G C C+HNTKG +CE+C DFY+D PW PA+ S+N C+KC CN HS+ CHFD VY + SGGVCD+CQHNT +NCE+CK +Y++ I C C+CD G++ IC + + +GL AG C CK NVEG +CD+CK GF+ N + GC C C +GT+ CDS G C+CK FVTG C C ++ LSN +++GC C C GS DN C SG+C C+ G +C+E++S +Y ++ TY PL H + I V + Y F I +M+ +D I V+++ GK S ++ L SRYI +P C EK L + L +++ SN K + N+ CL N ++V + D + T + KC+C+ GS ++ C+ GGQCQC PNV G+ C +CA ++GF GCK C+CD+ GS + C+ +TG+C C G G+ CD C PGYW FP C C+CN + C++ TG+C NCK +S GY+C+ C +GYYGD G C+ C CP G S + + GC T C C GY+G CD C + +FGN G CQPC C+ NID +C+ ++G C KCL++T G NC++C GY+GN + C +CVCN++GT + + CN G+CPCLPNV+G CDRC +NL+S GC PC CD +L SCN+ TGQC C G G+ C+ C++ FWG+P C C C+ G QCNR G C CK G+ G CD C +G G P C+ C C+ WD + + N +L N ++ + +S V P + +++ ++ ++ I + P + I N E+ + ++ + + + ++ + KA+ + KI +V GA + + K + L N I + +E+ N+ NL +++ ++ L++E++ N + + ++ T S L+C + CGG+ CK D R C G + + + T + L E + SK + ++ ++ E + ++ + T+ + + NL+ +I+ +L ADL E++ + K ++ ++ L ++IR R+ L+ V+ IL +S + + + A + + + + ++ + K + ++ + A +KG + E+EA + ++ T+R+ L+ I + K + Q ++ N KV+ + +N +K +K +E + K+ L + K + NKKL I D E + + ++EK +L+ L++ + +
Sbjct: 27 CAEGSCYPATGDLLIGRSDQLTASSTCGIQKPESFCIV--SHLQEEKKCFICDSLDPYNEQNNTESHRIENVVTTFAPNRLNLWWQSENGLENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWKIYRYFAYDCETSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIYRVLDPAFRIDDPYSLRIKS-MLRITNLRIKFLKLHTLGDNLLDSRVEIKEKYYYAIYDMVVRGNCFCYGHASECAPVKGYSDNVEGMVHGRCVCRHNTKGLNCEQCLDFYHDLPWRPAEGRSSNACKKCNCNEHSTHCHFDMAVYMSTGNTSGGVCDDCQHNTMGRNCEQCKPFFYQHPDKDIRDPNICQPCNCDPAGSQDGGICDRYSDFSVGLIAGQCRCKLNVEGERCDQCKEGFYDLNAEDPEGCQFCGCNPLGTIPGGNSCDSRNGNCFCKRFVTGRKCDQCAPEFWGLSN-DLDGCRPCDCDHGGSEDN-NCSPESGQCRCRPHMIGRQCNEVESGFYFISLDHYTYEAEYANFGPDVRIVERPLPHDRTPSWTGIGFARVPEGAYLEFHINDIPTSME-YDLLIRYEPQLPEQWEEAVITVIRPGKIPTSSRCGNTVPSDDNQSVALPPGSRYITLPRPICFEKGLNYTIRLELPRYSVNSNVENPYTLIDSIVLLPHVKSLDIFSMGGSGDDVTTNTAWQTFQRYRCLENSQSVVKTPLTDVCKNFLFTISALLHK--KCQCDPQGSLSSVCDPNGGQCQCRPNVIGRNCNKCAPGTFGFGPNGCKPCECDVRGSSHPFCHTETGQCPCIHGAFGRQCDVCLPGYWGFPSCRQCQCNGHAEDCNALTGECTNCKDHSEGYSCERCLSGYYGDPRLGSGDHCRPCPCPDGPGSERQFASGCYKDPTTLQVVCSCNMGYTGIRCDGCASGYFGNPEEIGGVCQPCHCNNNIDMSDSLACHKQSGLCLKCLYHTEGINCQECQLGYYGNAIQQDCRRCVCNYLGTEQENCSGEIDCHCNRTNGQCPCLPNVIGQSCDRCAANTWNLASGTGCEPCACDTN-RSLGLSCNEFTGQCQCMPGFGGRDCSECQELFWGNPDI--ECRACDCDSRGIQTSQCNRATGHCICKEGVEGVRCDKCARGYSGAFPYCTPCHKCFALWDIIIQELSNRTKQLLNRANDLKISGVIGPYQ--QTVDKVQEKLEEIRSILAHNPAAQPLKNIENLFEEAEKNLNQLFLKSEVSQKVDEKLDALQADAESFGKALKELGDQLEFMKISNVQGAMDSINKYFQMSLEAEEMVNASVSNPDSIIEHSAATRQEVENLINENLSQFKKEQEEQTRLLDELAGNGQSLDLSSLSEKTCGNSPGLLCEDSECGGLNCKTDDGKRKC-GGPGCGGLVTVAHSAWKTASSFDTEIQNALAEVEEL------SKMVAESKLRAEEAKQSAQDVLTKANATKEKVNQSNEDLRNLIKQIREFLMQDGADLDSIEAVASEVLKMDMPTTPEQLQKLTEEIRERVESLSGVDTILKQSAGDIARAEALLDEAKESSKSAADIKATVDAVKEALMEAGKAQSAAEKAI---KRADDDIKGTNDLLTSIESEAAVSEETLNNATKRLVQLENNIDDLMKKSAANSQTANQVEKSVHEVKANANGAKKVLDGELTNKYKTVEDLIAKKAEDSEEARRKAQELQKEAKG--LLEQANKKLDTIKDLEKTYEGNERILEEKAKQLVQLEETVRA 1753
BLAST of Netrin-4 vs. Ensembl Mouse
Match: Lamb1 (laminin B1 [Source:MGI Symbol;Acc:MGI:96743]) HSP 1 Score: 854.744 bits (2207), Expect = 0.000e+0 Identity = 547/1770 (30.90%), Postives = 871/1770 (49.21%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE--------------IKINLPP--------VSKSGYYNFVITTDTKNMQF-----FDSFI------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLG-----------------ECL-NYANLVSSKKCDF-LNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKW----QGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDK-LHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDI------------IISNFSEKLSRLKYYVY------NSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG-------ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFN-----GVTCNTITESNPLMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKP-ESEYNTTIKLI--DVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVN---KSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C P GDLLIGR L +STCGL E YC+V+ +K C ICDS++PY + SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + FKT+RPAAM IERS D+G+ W Y YF+ +C FP I +++++C S +S I PS G V+F A + D Y + +N + ITN+R++FV+LHT GD +D IR KYYYA+ + ++RG C CYGHAS+C +G +V+ MV+G C C+HNTKG +CE C DFY+D PW PA+ ++N C+KC CN HSSSCHFD V+ + +SGGVCDNCQHNT +NCE+CK Y+++ I C C CD G++ IC + +GL AG C CK +VEG +CD CK GF+ + + GC C C +GT+ CDS TG CYCK VTG C C ++ LSN +++GC C C + G N +C + SG+C C G +C+E++S +Y + + +E I+ +P V + Y F I +M++ ++ + V+++ GK S + L SRY+V+P C EK + + L +T ++ P ++ + S V G CL N ++V + D N S + + + C+C+ GS ++ C+ GGQCQC PNV G+TC RCA ++GF GCK C C L GS ++ C TG+C C +G+ + CD C PGYW FP C C+CN + CD+ TG+CL+C+ + G+NC+ C GYYGD G C+ C CP G S + + C T C+C GY G CD C + FGN G+CQPC+C NID ++C+ +TG C KCL++T G +C+ C GY+G+ + C KCVCN++GT + C+ TG+C CLPNV+G CDRC + L+S GC PCNC+ + SCN+ TGQC C G G+ C+ C++ FWGDP C C C+ G QC+++ G C C G+ G CD C +G G P+C+ C C+ WD + + N K L + + +S V P + S+E+ + +K+I P + + + +EK+++++ + NS + Q +++ + + K D+ GA + + K + L ++ + L +D++ E+L +++ ++ L++E++ + + + +TC T ++ CSE CGG C+ D+ + + ++ DR +S L E Q SK + +A V+ E++ N L+ + T+ + + NL+ +I+ +L SADL E++ + K E+ ++ L + IR R+ L+ V IL +S + + ++ + A RA + + + + + +K ++ ++ + A ++G + E+E + ++ ++RI L+ + ++ + + + + ++ + +N D K ++ Y K++S K E + K+ L ++ K + +L+ D E K + K +++K L+ L+ ++ S
Sbjct: 78 CAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKK--CFICDSRDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKAWGVYRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQN-LLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGVNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGRNCEQCKPFYFQHPERDIRDPNLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKEGFYDLSAEDPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSN-DLDGCRPCDC-DLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGPGVIVVERQYIQDRIPSWTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHWEKAVITVQRPGKIPASSRCGNTVPDDDNQVVSLSPGSRYVVLPRPVCFEKGMNYTVRLELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSGDGEVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLPGYWGFPSCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGSCQPCQCHHNIDTTDPEACDKETGRCLKCLYHTEGDHCQLCQYGYYGDALRQDCRKCVCNYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNTWQLASGTGCGPCNCNA-AHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDV--ECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKAKALKISGVIGPYR--ETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTEKMAQVEVKLTDTASQSNSTAGELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDSITKYFQMSLEAEKRVNASTTDPNSTVEQSALTRDRV----EDLMLERESPFKEQQEEQARLLDELAGKLQSLDLSAVAQMTCGTPPGAD---CSESECGGPNCRTDEGEKKCGGPGCGGLVTVAHSAWQKAMDFDRDVLS-ALAEVEQL------SKMVSEAKVRADEAKQNAQDVLLKTNATKEKVDKSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVETLSQVEVILQQSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAI---KQADEDIQGTQNLLTSIESETAASEETLTNASQRISKLERNVEELKRKAAQNSGEAEYIEKVVYSVKQNAD-DVKKTLDGELDEKYKKVESLIAQKTEESADA-RRKAELLQNEAKTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRS 1818
BLAST of Netrin-4 vs. Ensembl Mouse
Match: Lamb1 (laminin B1 [Source:MGI Symbol;Acc:MGI:96743]) HSP 1 Score: 854.744 bits (2207), Expect = 0.000e+0 Identity = 546/1767 (30.90%), Postives = 868/1767 (49.12%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE--------------IKINLPP--------VSKSGYYNFVITTDTKNMQF-----FDSFI------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLG-----------------ECL-NYANLVSSKKCDF-LNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKW----QGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDK-LHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDI------------IISNFSEKLSRLKYYVY------NSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG-------ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNP--LMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKP-ESEYNTTIKLI--DVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVN---KSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C P GDLLIGR L +STCGL E YC+V+ +K C ICDS++PY + SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + FKT+RPAAM IERS D+G+ W Y YF+ +C FP I +++++C S +S I PS G V+F A + D Y + +N + ITN+R++FV+LHT GD +D IR KYYYA+ + ++RG C CYGHAS+C +G +V+ MV+G C C+HNTKG +CE C DFY+D PW PA+ ++N C+KC CN HSSSCHFD V+ + +SGGVCDNCQHNT +NCE+CK Y+++ I C C CD G++ IC + +GL AG C CK +VEG +CD CK GF+ + + GC C C +GT+ CDS TG CYCK VTG C C ++ LSN +++GC C C + G N +C + SG+C C G +C+E++S +Y + + +E I+ +P V + Y F I +M++ ++ + V+++ GK S + L SRY+V+P C EK + + L +T ++ P ++ + S V G CL N ++V + D N S + + + C+C+ GS ++ C+ GGQCQC PNV G+TC RCA ++GF GCK C C L GS ++ C TG+C C +G+ + CD C PGYW FP C C+CN + CD+ TG+CL+C+ + G+NC+ C GYYGD G C+ C CP G S + + C T C+C GY G CD C + FGN G+CQPC+C NID ++C+ +TG C KCL++T G +C+ C GY+G+ + C KCVCN++GT + C+ TG+C CLPNV+G CDRC + L+S GC PCNC+ + SCN+ TGQC C G G+ C+ C++ FWGDP C C C+ G QC+++ G C C G+ G CD C +G G P+C+ C C+ WD + + N K L + + +S V P + S+E+ + +K+I P + + + +EK+++++ + NS + Q +++ + + K D+ GA + + K + L ++ + L +D++ E+L +++ ++ L++E++ + + + +T P CSE CGG C+ D+ + + ++ DR +S L E Q SK + +A V+ E++ N L+ + T+ + + NL+ +I+ +L SADL E++ + K E+ ++ L + IR R+ L+ V IL +S + + ++ + A RA + + + + + +K ++ ++ + A ++G + E+E + ++ ++RI L+ + ++ + + + + ++ + +N D K ++ Y K++S K E + K+ L ++ K + +L+ D E K + K +++K L+ L+ ++ S
Sbjct: 30 CAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKK--CFICDSRDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKAWGVYRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQN-LLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGVNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGRNCEQCKPFYFQHPERDIRDPNLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKEGFYDLSAEDPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSN-DLDGCRPCDC-DLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGPGVIVVERQYIQDRIPSWTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHWEKAVITVQRPGKIPASSRCGNTVPDDDNQVVSLSPGSRYVVLPRPVCFEKGMNYTVRLELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSGDGEVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLPGYWGFPSCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGSCQPCQCHHNIDTTDPEACDKETGRCLKCLYHTEGDHCQLCQYGYYGDALRQDCRKCVCNYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNTWQLASGTGCGPCNCNA-AHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDV--ECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKAKALKISGVIGPYR--ETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTEKMAQVEVKLTDTASQSNSTAGELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDSITKYFQMSLEAEKRVNASTTDPNSTVEQSALTRDRV----EDLMLERESPFKEQQEEQARLLDELAGKLQSLDLSAVAQMTCGTPPGADCSESECGGPNCRTDEGEKKCGGPGCGGLVTVAHSAWQKAMDFDRDVLS-ALAEVEQL------SKMVSEAKVRADEAKQNAQDVLLKTNATKEKVDKSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVETLSQVEVILQQSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAI---KQADEDIQGTQNLLTSIESETAASEETLTNASQRISKLERNVEELKRKAAQNSGEAEYIEKVVYSVKQNAD-DVKKTLDGELDEKYKKVESLIAQKTEESADA-RRKAELLQNEAKTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRS 1770
BLAST of Netrin-4 vs. Ensembl Mouse
Match: Lamb2 (laminin, beta 2 [Source:MGI Symbol;Acc:MGI:99916]) HSP 1 Score: 770.77 bits (1989), Expect = 0.000e+0 Identity = 431/1169 (36.87%), Postives = 626/1169 (53.55%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE-MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEW---YIEKPTYP--------LEHLKEIKIN--LPPVSKSGY--------YNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQS-------YQDTMQ--LIKDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXX----------------------XXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGI-----CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFL 3410
C + C P GDLL+GR L ASSTCGL + + YC+V+ + KC +CDS+ P+ + +SHRI+NVV+ PQ WWQS +GV V+I++ E+EF FT + FKT+RPAAM +ERS D+GRTW+ Y YFS +C DFP IP ++V+C S +S I PS G V++ D Y ++ +N + ITN+RV RLHT GD +D IR KYYYA+ E +IRG C CYGHAS+C G E MV+G C C+HNT+G +CE+C+DFY D PW PA+ T+ CRKCECN H+ SCHFD VY S +SGGVCD CQHNT ++CE C+ +YR+ + C C CD +G++ C + LGL +G C CKE+V G +C +C+ GF+ + ++ GC +C C GT+ CDSS+G C+CK VTG+ C C G++ LS+ ++ GC C C G+ D + CD+ +G+C C+ G RC++++ + +++ T+ LE ++ + N P + G+ F++T+ + M +D + + L + G S S D +Q L ++R++V P CLE + L + I + + P L + + + E L + S+ C L IS + + + + C+C+ GS ++ C+ GGQC+C P V G+ C+ CA+ YGF GC+ C+C G+L++ C +G+C CR G G CD C+ G W FP C C CN + CD+ TG CL C+ + G +C+ C G++GD G C+ C CP G S ++ + C + C C++GY+G C+ C HFG+ G CQ C CSGNID D+C+ TG+C +CL NT G +C C G+ G A ++C +C CN +GT+ + C+ TG+CPCLP+V GL CD C F+N +S GC PC C +P A +CN+ TGQC+C AG G+ C+ C++ +WGDP C C C+ G QC+R+ G C C+PG++G CD C +G G P C C C+ WD +
Sbjct: 45 CSRGSCYPATGDLLVGRADRLTASSTCGLHSPQPYCIVSHLQD--EKKCFLCDSRRPFSARDNPNSHRIQNVVTSFAPQRRTAWWQSENGVPMVTIQLDLEAEFHFTHLIMTFKTFRPAAMLVERSADFGRTWHVYRYFSYDCGADFPGIPLAPPRRWDDVVCESRYSEIEPSTEGEVIYRVLDPAIPIPDPYSSRIQN-LLKITNLRVNLTRLHTLGDNLLDPRREIREKYYYALYELVIRGNCFCYGHASQCAPAPGAPAHAEGMVHGACICKHNTRGLNCEQCQDFYQDLPWHPAEDGHTHACRKCECNGHTHSCHFDMAVYLASGNVSGGVCDGCQHNTAGRHCEFCRPFFYRDPTKDMRDPAVCRPCDCDPMGSQDGGRCDSHDDPVLGLVSGQCRCKEHVVGTRCQQCRDGFFGLSASDPRGCQRCQCNSRGTVPGSSPCDSSSGTCFCKRLVTGHGCDRCLPGHWGLSH-DLLGCRPCDCDVGGALDPQ-CDEATGQCRCRQHMIGRRCEQVQPGYFRPFLDHLTWEAEAAQGQGLEVVERLVTNRETPSWTGPGFVRLREGQEVEFLVTSLPRAMD-YDLLLRWEPQVPEQWAELELMVQRPGPVSAHSPCGHVLPKDDRIQGMLHPNTRFLVFPRPVCLEPGISYKLKLKLIGTGGRAQPETSYSGLLIDSLVLQPHVLVLEMFSGGDAAALERRTTFERYRCHEEGLMPSKAPLSETCAPLL--ISVSALIYNGALPCQCDPQGSLSSECSPHGGQCRCKPGVVGRRCDVCATGYYGFGPAGCQACQCSPDGALSALCEGTSGQCPCRPGAFGLRCDHCQRGQWGFPNCRPCVCNGRADECDTHTGACLGCRDYTGGEHCERCIAGFHGDPRLPYGGQCRPCPCPEGPGSQRHFATSCHRDGYSQQIVCQCREGYTGLRCEACAPGHFGDPSKPGGRCQLCECSGNIDPMDPDACDPHTGQCLRCLHNTEGPHCGYCKPGFHGQAARQSCHRCTCNLLGTDPRRCPSTDLCHCDPSTGQCPCLPHVQGLNCDHCAPNFWNFTSGRGCQPCAC-HPSRARGPTCNEFTGQCHCHAGFGGRTCSECQELYWGDPGLQ--CRACDCDPRGIDKPQCHRSTGHCSCRPGVSGVRCDQCARGFSGVFPACHPCHACFGDWDRVV 1202
BLAST of Netrin-4 vs. Ensembl Mouse
Match: Lama2 (laminin, alpha 2 [Source:MGI Symbol;Acc:MGI:99912]) HSP 1 Score: 363.614 bits (932), Expect = 4.218e-103 Identity = 318/1137 (27.97%), Postives = 489/1137 (43.01%), Query Frame = 3
Query: 168 LLIGRESFLRASSTCGLTNSERYCVVTTSDSG---RKSKCTICD--SKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMS---GV--HNVSIEISFESEFQFTFTHLRFK-TYRPAAMYIERSKD--YGRTWNKYAYFSDNCP---RDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTF-GDWKV-------DDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQ-------SRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQ-EPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKS---GYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLT---VFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDAR----GCKKCKCDLTGSLNSQCYNDTGKCL-CREGVEGQHCDTCKPGYWNFPF------CHSCKC------NQMSTTCDSKTGKCLNCKGNSFGYN---CQNCENGYYGDTS-RGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFT---CMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWL 3392
L + + + ++TCG E YC + G R +C IC+ S NPYQR T++ +N WWQS S GV H V+I + + FQ + ++ + RP +ERS D + W +A C +P + +EV+CTS +S I P E G + S +D + + + T+ IR++F R+ T D + + +P + +YYY++ + + G C CYGHA C + + C+C+HNT G+ C++C ++ PW + +EC C C+ + C++D V + + + GGVC NC HNT NCE C G++R V+ N + C CHCD G+ +C++ E GL G C CK G CD+C G+ + C CNC +G+ D G C CK V G DCS CK G+FNL N GC C CS +R + Y + + I+ ++ P P ++I I+ KS GYY N + +I + + + +QL+ I+ DL I V+ S I + + N TRK L V + + + + + + + + V + CQC P SG +CE C + C+ C+C + C + TG+CL C++ G +C+ C PG++ P C C C N S TC L C GY C+ C GY+G S G C+ CQC N+++ S+ + + + +CK G +G++C+ C +FG+A++ CQPCRC NI+ + C+++TG+C +C N G +C++C FG R C C CN G+ S +G+C C P V G +CDRC +FN + GC+ C+C + L ++C+ TGQC C +G+ C+ C WG + + GC C C+ GS+ QCN N G C C P +G C C +G P C+ C DC+L
Sbjct: 40 LNLASNALITTNATCGEKGPEMYCKLVEHVPGQPVRNPQCRICNQNSSNPYQRHPITNAIDGKNT----------WWQSPSIKNGVEYHYVTITLDLQQVFQIAYVIVKAANSPRPGNWILERSLDDVEYKPWQYHAVTDTECLTLYNIYPRTGPPSYAKDDEVICTSFYSKIHPLENGEIHISLINGRPSADDP--SPELLEFTSARYIRLRFQRIRTLNADLMMFAHKDPREIDPIVTRRYYYSVKDISVGGMCICYGHARACPLDPATNKSR------CECEHNTCGESCDRCCPGFHQKPWRAGTFLTKSECEACNCHGKAEECYYDETVASRNLSLNIHGKYIGGGVCINCTHNTAGINCETCVDGFFRPKGVSPNYPRPCQPCHCDPTGS-LSEVCVKDEKYAQRGLKPGSCHCKTGFGGVNCDRCVRGYHGY-----PDCQPCNCSGLGSTNE---DPCVGPCSCKENVEGEDCSRCKSGFFNLQEDNQKGCEECFCSGVSNRCQSSYWTYGNIQDMRGWYLTDLSGRIRMAPQLDNPDSP----QQISISNSEARKSLLDGYYWSAPPPYLGNRLPAVGGQLSFTISYDLEEEEDDTEKILQLM-----IIFEG----NDLRISTAYKEVYLEPSEEHIEEVSLKEEAFTIHGTNLPVTRKDFMIVLTNLERVLMQ----ITYNLGMDAIFRLSSVNLESAVPYPTDRRIATDVEVCQCPPGYSGSSCETCWPRHRRVNGTIFGGICEPCQC---FAHAEACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQC-------NDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAK-NCQPCRC--NINGSFSEICHTRTGQC-ECRPNVQGRHCDECKPETFGLQLGRGCLPCNCNSFGSKS----FDCEASGQCWCQPGVAGKKCDRCAHGYFNF-QEGGCIACDCSH----LGNNCDPKTGQCICPPNTTGEKCSECLPNTWGH-SIVTGCKVCNCSTVGSLASQCNVNTGQCSCHPKFSGMKCSECSRG-HWNYPLCTLC-DCFL 1106 HSP 2 Score: 160.229 bits (404), Expect = 9.059e-39 Identity = 156/570 (27.37%), Postives = 230/570 (40.35%), Query Frame = 3
Query: 2205 CNKIGGQC-QCLPNVSGKTCERCASESYGFDARG----CKKCKCDL---TGSLNSQCYNDTGKCL----CREGVEGQHCDTCKPGYWNFPF-----CHSCKCN-----QMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRK-----CLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNR---------NNGVCRCKPGITGRNCDMCMK---GTDGKMP-NCSSC------GDC---------WLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVY--NSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAF 3743
C+ I G+C C + G C C YG RG C+ C C L + + + C+ D L C G G C+ C GY+ P C C+CN + +CDS +G CL CK + G C+ C +GY+GD C+ C+C N + S I +T C C+ G+ CD+C+ FG + + C PC C +S SK+ +C C +G C++C GYF N C C C+H+G N C+ +TG+C C PN G +C C + S GC CNC +G+L CN TGQC C SG C+ C G W P CT C C L G+ C+ G C CK + G +CD C G D K P CSSC C W+ ++ + L +++ + F K ++ +++ + L Y+ + +LKY +Y + F T + +I I T +I+++ + G
Sbjct: 762 CDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRC-------NINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQLGRG--CLPCNC---------NSFGSKSFDCEASGQCWCQPGVAGKKCDRCAHGYF-NFQEGGCIACDCSHLGNN------CDPKTGQCICPPNTTGEKCSECLPNTWGHSIVTGCKVCNCST-VGSLASQCNVNTGQCSCHPKFSGMKCSECSRGHWNYPL----CTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCRPGKFGLDAKNPLGCSSCYCFGVTSQCSEAKGLIRTWVTLSDEQTILPLVDEALQHTT---TKGIAFQKP--EIVAKMDEVRQELHLEPFYWKLPQQFEGKKLMAYGGKLKYAIYFEARDETGFATYKPQVI-IRGGTPTHARIITRHMAAPLIGQL 1295 HSP 3 Score: 132.88 bits (333), Expect = 2.019e-30 Identity = 127/443 (28.67%), Postives = 196/443 (44.24%), Query Frame = 3
Query: 2061 VLGECLNYANLVSSKKCD----FLNAEISTNTVTKSDPIKCKCNVTGSR-NNNCN---KIGGQC-QCLPNVSGKTCERCASESYG---FDARGCKKCKCD--LTGSLNSQCYNDTGKCL-CREGVEGQHCDTCKPGYW----NFPFCHSCKCN---QMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYP--------IGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWG-DPTKLNGCTKCYCNLDGSVFKQCNRNNGVCR 3296
+ GECLN + C+ + + + P C N+ + + C+ +G C +C +G CERCA +G C+ C+C+ L S+ C + +G CL C+ G G++C+ C GY+ N C C+CN S C ++TG+C C+ N G +C C+ +G G C C C NS G + C C+ G +G+ CD+C +F ++G C C CS + ++C+ KTG+C C NT+G C +C+ +G+ V C C C+ +G+ + CN TG+C C P G++C C +N C C+C P + SC+ TGQC C+ G C+ C+ G +G D GC+ CYC V QC+ G+ R
Sbjct: 765 ITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDECPIGYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPGSCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRCNINGSFSEICHTRTGQC-ECRPNVQGRHCDECKPETFG-LQLGRGCLPCNC--------NSFGSKSFDCEASGQCWCQPGVAGKKCDRCAHGYFN---FQEGGCIACDCS-----HLGNNCDPKTGQC-ICPPNTTGEKCSECLPNTWGHSIVTGCKVCNCSTVGSLASQ---CNVNTGQCSCHPKFSGMKCSECSRGHWNYPL---CTLCDCFLPGTDATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCRPGKFGLDAKNPLGCSSCYCF---GVTSQCSEAKGLIR 1179 HSP 4 Score: 122.865 bits (307), Expect = 2.419e-27 Identity = 87/280 (31.07%), Postives = 127/280 (45.36%), Query Frame = 3
Query: 2658 TTAFTCMCKKGYSGQFCDQCETNHFG-NAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGN---GAVRTCTKCVCNHMGTNSKWQGICNHETG------KCPCLPNVVGLQCDRCQDKFFNLSSQIG--CLPCNC----DYPIGALNHSCNQLTGQCY-CQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDG-----------------KMPNCSSCGDCWLQ 3395
T C C GYSG C+ C H N G C+PC+C + ++C+ TGEC C +T G C +C+ G++G+ G+ C C C ++ + C+ + +CP G +C+RC + +F S G C PC C DY I SC+ L+G C C+ G +G+ C C DG++GD C C CN++GS + C+ G C C+P + GR+CD C T G K +C + G CW Q
Sbjct: 715 TDVEVCQCPPGYSGSSCETCWPRHRRVNGTIFGGICEPCQCFAHA-----EACDDITGECLNCKDHTGGPYCNECLPGFYGDPTRGSPEDCQPCACPLNIPSNNFSPTCHLDRSLGLICDECPI--GYTGPRCERCAEGYFGQPSIPGGSCQPCQCNDNLDYSIPG---SCDSLSGSCLICKPGTTGRYCELCADGYFGDAVNAKNCQPCRCNINGSFSEICHTRTGQCECRPNVQGRHCDECKPETFGLQLGRGCLPCNCNSFGSKSFDCEASGQCWCQ 984 HSP 5 Score: 81.2629 bits (199), Expect = 1.163e-14 Identity = 77/259 (29.73%), Postives = 110/259 (42.47%), Query Frame = 3
Query: 933 GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCD---------KYSGKCLCKNGFAGIRCDEIK 1682
G C+C+ N +G+HC++CK ++ L + C C CNS S FD + G C CQ K C+RC GY+ C+ C C LG + G CIC N G +C +C W + TGC CNC +G+L ++ C+ +TG+C C +G CS C G++N C+LC C G+ D TCD +G+C CK G+ CD +
Sbjct: 931 GQCECRPNVQGRHCDECKP---ETFGLQLGRG----CLPCNCNSFGSK-SFD--------CEASGQC-WCQPGVAGKKCDRCAHGYFNF------QEGGCIACDCSHLGN------------NCDPKTGQCICPPNTTGEKCSECLPNTWGHS--IVTGCKVCNCSTVGSLASQ-CNVNTGQCSCHPKFSGMKCSECSRGHWNYPL-----CTLCDCFLPGT-DATTCDLETRKCSCSDQTGQCSCKVNVEGVHCDRCR 1145
BLAST of Netrin-4 vs. Ensembl Mouse
Match: Lamb3 (laminin, beta 3 [Source:MGI Symbol;Acc:MGI:99915]) HSP 1 Score: 353.984 bits (907), Expect = 8.132e-101 Identity = 194/538 (36.06%), Postives = 284/538 (52.79%), Query Frame = 3
Query: 63 MTLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLC--TSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRC 1670
MT ++ + + ++ + C + C P GDLLIGR LRASSTCGLT E YC T + KC CDS+ P + +SHR+ENV S P RWWQS + V VS+++ + Q + FK PA M IERS D+G+TW Y Y + +C FP + + + N +V C S+ + + G L D + + +KK ++ ITN+RV F +L V + Y+A+S+ ++G C C+GHA +C G V VC CQHNT G +C++C FYN+ PW PA+ T+EC++C+CN HS +CHFDP V+ S+ +GGVCDNC+ +TE KNCERC+ Y+RN + +TC+ C CD G + C + G C+CKE V+G +CD CK GF GC C+C ++G ++ C+ TG+C C V G C C ++ L++ GC C C S ++ C++++G+C C+ GF G+ C
Sbjct: 1 MTAFFLLWLALPGFLC---AQQACSRGACYPPVGDLLIGRTQLLRASSTCGLTKPETYC---TQYGQWQMKCCKCDSRLP----RNYNSHRVENVASS--SGPMRWWQSQNDVSPVSLQLDLDKRMQLQDIMMDFKGLTPAGMLIERSSDFGKTWRVYQYLATDCASTFPQVHQGQPKNWQDVRCRPLSQRPNGHLTGGKVQLNLMDLASAI--PASQSKKIQELGDITNLRVNFTKLAP-----VPQRGSYPPSAYFAVSQLRLQGSCFCHGHADRCAPNPGGSTTAVQVNNVCVCQHNTAGPNCDRCAPFYNNRPWRPAEGQDTHECQRCDCNGHSETCHFDPAVFAASQGTNGGVCDNCRDHTEGKNCERCQLHYFRNRRPSAPIHETCIPCECDPDGAVQGAPCDR--------LTGQCVCKEYVQGERCDLCKPGFTGLTFANPKGCHACDCSILGARKDMPCEEETGRCLCLPNVVGPKCDQCAPSHWKLASG--LGCEPCACDPRNSLSSQ-CNQFTGQCPCREGFGGLTC 508 HSP 2 Score: 142.895 bits (359), Expect = 1.464e-33 Identity = 116/415 (27.95%), Postives = 174/415 (41.93%), Query Frame = 3
Query: 2370 CLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGN---AISKDGTCQPCRCSGNIDQRIEDS-CNSKTGECRKCLFNTSGFNCEKCVSGYFGN--GAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNN-----CKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNS-----SGVFMSNVKFPKEIDDVCTSLEQNMKSLK 3536
C+C+ G +CD C P Y N P+ C C CN S TC +G + G C+N C+ G+ C++C+ ++F N + TC PC C + D ++ + C+ TG+C C G C+ C G+ G + C C C+ +G C ETG+C CLPNVVG +CD+C + L+S +GC PC CD P +L+ CNQ TGQC C+ G G C++ C D +G GC C C+ G+ C++ +G C C+PG TG CD C +G + P C +C C+ +D L L N+ +G + + + D + +EQ + L+
Sbjct: 276 CVCQHNTAGPNCDRCAPFYNNRPWRPAEGQDTHECQRCDCNGHSETCHFDPAVFAASQGTNGGV----CDN------------------------------------------CRDHTEGKNCERCQLHYFRNRRPSAPIHETCIPCEC--DPDGAVQGAPCDRLTGQC-VCKEYVQGERCDLCKPGFTGLTFANPKGCHACDCSILGARKDMP--CEEETGRCLCLPNVVGPKCDQCAPSHWKLASGLGCEPCACD-PRNSLSSQCNQFTGQCPCREGFGGLTCSSAAIRQCPDQTYGHVA--TGCRACDCDFRGTEGPGCDKASGRCLCRPGFTGPRCDQCQRGHCDRYPVCVACHSCFQAYDTDLQEQARRLHSLRNATEGLWTGTGLEDHGLASRLLDAKSKIEQIRQILE 636 HSP 3 Score: 103.99 bits (258), Expect = 1.330e-21 Identity = 70/227 (30.84%), Postives = 111/227 (48.90%), Query Frame = 3
Query: 1050 CECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQ---TCLKCHCDSLGTKFPSICIQEPEL---DLGLNAGHCI-CKENVEGNQCDKCKIGFWK---FNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE-IKSEWYI 1697
C C+ H+ C +P + +++ VC CQHNT NC+RC + +Y N Q C +C C+ C +P + G N G C C+++ EG C++C++ +++ + C+ C C G ++ CD TG+C CK +V G C CK G+ L+ AN GC C CS G+R + C++ +G+CLC G +CD+ S W +
Sbjct: 250 CFCHGHADRCAPNPGGSTTAVQVNN-VC-VCQHNTAGPNCDRC-APFYNNRPWRPAEGQDTHECQRCDCNG----HSETCHFDPAVFAASQGTNGGVCDNCRDHTEGKNCERCQLHYFRNRRPSAPIHETCIPCECDPDGAVQGAPCDRLTGQCVCKEYVQGERCDLCKPGFTGLTFANPKGCHACDCSILGARKDMPCEEETGRCLCLPNVVGPKCDQCAPSHWKL 469 HSP 4 Score: 89.3521 bits (220), Expect = 3.586e-17 Identity = 62/205 (30.24%), Postives = 95/205 (46.34%), Query Frame = 3
Query: 2163 IKCKCNVTGS-RNNNCNKIGGQCQCLPNVSGKTCERCASESYGF---DARGCKKCKCDLTGSL-NSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF---CHSCKC---NQMSTTCDSKTGKCLNCKGNSFGYNC-----QNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNH 2729
I C+C+ G+ + C+++ GQC C V G+ C+ C G + +GC C C + G+ + C +TG+CLC V G CD C P +W C C C N +S+ C+ TG+C C+ G C + C + YG + G C+ C C GC + + C+C+ G++G CDQC+ H
Sbjct: 374 IPCECDPDGAVQGAPCDRLTGQCVCKEYVQGERCDLCKPGFTGLTFANPKGCHACDCSILGARKDMPCEEETGRCLCLPNVVGPKCDQCAPSHWKLASGLGCEPCACDPRNSLSSQCNQFTGQC-PCREGFGGLTCSSAAIRQCPDQTYGHVATG--CRACDC---DFRGTEGPGC----DKASGRCLCRPGFTGPRCDQCQRGH 568 HSP 5 Score: 83.5741 bits (205), Expect = 2.342e-15 Identity = 103/384 (26.82%), Postives = 150/384 (39.06%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCAS--------ESYGFDARGCKKCKC---------DLTGSLNSQCYNDTGKCL-CREGVEGQHCDTCKPGYW-----NFPF---CHSCKCNQ----MSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT---SRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNC-----EKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCD-YPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFW 3200
+C N GS ++ C C N +G C+RCA + G D C++C C D SQ N G C CR+ EG++C+ C+ Y+ + P C C+C+ CD TG+C+ CK G C C+ G+ G T +G C C C L + K+ + C+C G CDQC +H+ ++ C+PC C + + CN TG+C C G C +C +G+ A C C C+ GT C+ +G+C C P G +CD+CQ +CD YP+ HSC Q + R N +G W
Sbjct: 258 RCAPNPGGS--TTAVQVNNVCVCQHNTAGPNCDRCAPFYNNRPWRPAEGQDTHECQRCDCNGHSETCHFDPAVFAASQGTNG-GVCDNCRDHTEGKNCERCQLHYFRNRRPSAPIHETCIPCECDPDGAVQGAPCDRLTGQCV-CKEYVQGERCDLCKPGFTGLTFANPKG--CHACDCSILGARKD------MPCEEETGRCLCLPNVVGPKCDQCAPSHW--KLASGLGCEPCAC--DPRNSLSSQCNQFTGQC-PCREGFGGLTCSSAAIRQCPDQTYGHVAT-GCRACDCDFRGTEGPG---CDKASGRCLCRPGFTGPRCDQCQRG-------------HCDRYPVCVACHSCFQAYDTDLQEQARRLHSLRNATEGLW 607
BLAST of Netrin-4 vs. UniProt/SwissProt
Match: sp|P07942|LAMB1_HUMAN (Laminin subunit beta-1 OS=Homo sapiens OX=9606 GN=LAMB1 PE=1 SV=2) HSP 1 Score: 866.685 bits (2238), Expect = 0.000e+0 Identity = 550/1767 (31.13%), Postives = 870/1767 (49.24%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE--------------IKINLPP--------VSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKD----LPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLG-----------------ECL-NYANLVSSKKCDF-LNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT------NSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKN-TFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDI------------IISNFSEKLSRLKYYV------YNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG-------ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNP--LMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLL-LDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASN--SKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C P GDLLIGR L +STCGL E YC+V+ +K C IC+S++PY + SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FP I +++++C S +S I PS G V+F A + D Y + +N + ITN+R++FV+LHT GD +D IR KYYYA+ + ++RG C CYGHAS+C +G +V+ MV+G C C+HNTKG +CE C DFY+D PW PA+ ++N C+KC CN HS SCHFD VY + +SGGVCD+CQHNT +NCE+CK YY++ I C +C CD G++ IC + GL AG C CK NVEG CD CK GF+ + + GC C C +GT+ CDS TG CYCK VTG C C ++ LSN +++GC C C + G N +C SG+C C+ G +C+E++ +Y + L +E I+ +P V + Y F I +M+ +D I V+++ G+ S + L SRY+V+P C EK + + L +T ++ P + ++ + S V G CL N ++V + D N S + + + C+C+ GS ++ C+ GGQCQC PNV G+TC RCA ++GF GCK C+C L GS+N+ C TG+C C +GV + CD C PG+W FP C C+CN + CD TG+CLNC+ + G+NC+ C GYYGD G C+ C CP G S + + C T C+C GY G CD C + +FGN G+CQPC+C NID ++C+ +TG C KCL++T G +C+ C GY+G+ + C KCVCN++GT S Q C+ TG+C CLPNV+G CDRC + L+S GC PCNC+ + SCN+ TGQC C G G+ C+ C++ FWGDP C C C+ G QC+++ G C C G+ G CD C +G G P+C+ C C+ WD + + N T L + + +S V P + S+E+ + +K+I P + +I + +E +++++ + NS + ++ Q +++N+ + K D+ GA + + K + L +E + L++D++ E++ ++K ++ L++E++ + + + +T P CSE CGG C+ D+ R C G N + + LD+ +S + + +Q + ++ +++ K +E + + T+ + E NL+ +I+ +L SADL E++ + K E+ ++ L + IR R+ L+ V IL S + + ++ + A RA + + + + + +K ++ ++ + A ++G + E+E + + ++RI L+ + ++ + + + + ++ ++ + D K ++ K K N +K +E K+ L ++ K + +L+ D E K + + +++K L L+ ++ S
Sbjct: 30 CAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKK--CFICNSQDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWGVYRYFAYDCEASFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQN-LLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGFNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSISCHFDMAVYLATGNVSGGVCDDCQHNTMGRNCEQCKPFYYQHPERDIRDPNFCERCTCDPAGSQNEGICDSYTDFSTGLIAGQCRCKLNVEGEHCDVCKEGFYDLSSEDPFGCKSCACNPLGTIPGGNPCDSETGHCYCKRLVTGQHCDQCLPEHWGLSN-DLDGCRPCDC-DLGGALNNSCFAESGQCSCRPHMIGRQCNEVEPGYYFATLDHYLYEAEEANLGPGVSIVERQYIQDRIPSWTGAGFVRVPEGAYLEFFIDNIPYSME-YDILIRYEPQLPDHWEKAVITVQRPGRIPTSSRCGNTIPDDDNQVVSLSPGSRYVVLPRPVCFEKGTNYTVRLELPQYTSSDSDVESPYTLIDSLVLMPYCKSLDIFTVGGSGDGVVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALLHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPSGCKPCECHLQGSVNAFCNPVTGQCHCFQGVYARQCDRCLPGHWGFPSCQPCQCNGHADDCDPVTGECLNCQDYTMGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGYFGNPSEVGGSCQPCQCHNNIDTTDPEACDKETGRCLKCLYHTEGEHCQFCRFGYYGDALQQDCRKCVCNYLGTVQEHCNGSDCQ--CDKATGQCLCLPNVIGQNCDRCAPNTWQLASGTGCDPCNCNA-AHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDV--ECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDVIIAELTNRTHRFLEKAKALKISGVIGPYR--ETVDSVERKVSEIKDILAQSPAAEPLKNIGNLFEEAEKLIKDVTEMMAQVEVKLSDTTSQSNSTAKELDSLQTEAESLDNTVKELAEQLEFIKNSDIRGALDSITKYFQMSLEAEERVNASTTEPNSTVEQSALMRDRV----EDVMMERESQFKEKQEEQARLLDELAGKLQSLDLSAAAEMTCGTPPGASCSETECGGPNCRTDEGERKC-GGPGCGGLVTVAHNAWQKAMDLDQDVLSALAE--VEQLSKMVSEAKLRADEAKQSAE--DILLKTNATKEKMDKSNEELRNLIKQIRNFLTQDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVESLSQVEVILQHSAADIARAEMLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAI---KQADEDIQGTQNLLTSIESETAASEETLFNASQRISELERNVEELKRKAAQNSGEAEYIEKVVYTVKQSAE-DVKKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRS 1770
BLAST of Netrin-4 vs. UniProt/SwissProt
Match: sp|P02469|LAMB1_MOUSE (Laminin subunit beta-1 OS=Mus musculus OX=10090 GN=Lamb1 PE=1 SV=3) HSP 1 Score: 854.744 bits (2207), Expect = 0.000e+0 Identity = 546/1767 (30.90%), Postives = 868/1767 (49.12%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE--------------IKINLPP--------VSKSGYYNFVITTDTKNMQF-----FDSFI------IVLSIYHVGKSSCQSY---------QDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLG-----------------ECL-NYANLVSSKKCDF-LNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKW----QGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDK-LHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDI------------IISNFSEKLSRLKYYVY------NSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG-------ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNP--LMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKP-ESEYNTTIKLI--DVTRGAIQSRRIESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVN---KSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C P GDLLIGR L +STCGL E YC+V+ +K C ICDS++PY + SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + FKT+RPAAM IERS D+G+ W Y YF+ +C FP I +++++C S +S I PS G V+F A + D Y + +N + ITN+R++FV+LHT GD +D IR KYYYA+ + ++RG C CYGHAS+C +G +V+ MV+G C C+HNTKG +CE C DFY+D PW PA+ ++N C+KC CN HSSSCHFD V+ + +SGGVCDNCQHNT +NCE+CK Y+++ I C C CD G++ IC + +GL AG C CK +VEG +CD CK GF+ + + GC C C +GT+ CDS TG CYCK VTG C C ++ LSN +++GC C C + G N +C + SG+C C G +C+E++S +Y + + +E I+ +P V + Y F I +M++ ++ + V+++ GK S + L SRY+V+P C EK + + L +T ++ P ++ + S V G CL N ++V + D N S + + + C+C+ GS ++ C+ GGQCQC PNV G+TC RCA ++GF GCK C C L GS ++ C TG+C C +G+ + CD C PGYW FP C C+CN + CD+ TG+CL+C+ + G+NC+ C GYYGD G C+ C CP G S + + C T C+C GY G CD C + FGN G+CQPC+C NID ++C+ +TG C KCL++T G +C+ C GY+G+ + C KCVCN++GT + C+ TG+C CLPNV+G CDRC + L+S GC PCNC+ + SCN+ TGQC C G G+ C+ C++ FWGDP C C C+ G QC+++ G C C G+ G CD C +G G P+C+ C C+ WD + + N K L + + +S V P + S+E+ + +K+I P + + + +EK+++++ + NS + Q +++ + + K D+ GA + + K + L ++ + L +D++ E+L +++ ++ L++E++ + + + +T P CSE CGG C+ D+ + + ++ DR +S L E Q SK + +A V+ E++ N L+ + T+ + + NL+ +I+ +L SADL E++ + K E+ ++ L + IR R+ L+ V IL +S + + ++ + A RA + + + + + +K ++ ++ + A ++G + E+E + ++ ++RI L+ + ++ + + + + ++ + +N D K ++ Y K++S K E + K+ L ++ K + +L+ D E K + K +++K L+ L+ ++ S
Sbjct: 30 CAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKK--CFICDSRDPYHETLNPDSHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKAWGVYRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQN-LLKITNLRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGVNEEVEGMVHGHCMCRHNTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGRNCEQCKPFYFQHPERDIRDPNLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKEGFYDLSAEDPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSN-DLDGCRPCDC-DLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGPGVIVVERQYIQDRIPSWTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHWEKAVITVQRPGKIPASSRCGNTVPDDDNQVVSLSPGSRYVVLPRPVCFEKGMNYTVRLELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSGDGEVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQCQCRPNVVGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLPGYWGFPSCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARSCYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGSCQPCQCHHNIDTTDPEACDKETGRCLKCLYHTEGDHCQLCQYGYYGDALRQDCRKCVCNYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNTWQLASGTGCGPCNCNA-AHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDV--ECRACDCDPRGIETPQCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKAKALKISGVIGPYR--ETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTEKMAQVEVKLTDTASQSNSTAGELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDSITKYFQMSLEAEKRVNASTTDPNSTVEQSALTRDRV----EDLMLERESPFKEQQEEQARLLDELAGKLQSLDLSAVAQMTCGTPPGADCSESECGGPNCRTDEGEKKCGGPGCGGLVTVAHSAWQKAMDFDRDVLS-ALAEVEQL------SKMVSEAKVRADEAKQNAQDVLLKTNATKEKVDKSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVETLSQVEVILQQSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAI---KQADEDIQGTQNLLTSIESETAASEETLTNASQRISKLERNVEELKRKAAQNSGEAEYIEKVVYSVKQNAD-DVKKTLDGELDEKYKKVESLIAQKTEESADA-RRKAELLQNEAKTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRS 1770
BLAST of Netrin-4 vs. UniProt/SwissProt
Match: sp|A4D0S4|LAMB4_HUMAN (Laminin subunit beta-4 OS=Homo sapiens OX=9606 GN=LAMB4 PE=2 SV=1) HSP 1 Score: 786.178 bits (2029), Expect = 0.000e+0 Identity = 541/1783 (30.34%), Postives = 859/1783 (48.18%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENV-VSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSA-DTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIR-NKYYYAISEWIIRGRCSCYGHASKCKAKE---GQV-QVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKE----------------------IKINLP----PVSKSG---------------------YYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSC-----QSYQDTMQLIKDSRYIVIPNK-CLEKDLPIVLTVF---TIESNNKXXXXXXXXXXXXXXXXNSTK---TRKVLGE-----CLNYANLVSSK----KCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSK------WQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTS----LEQNMKSLKEIKKY--FPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG----ASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSE---------FCGGVGCKPDKCYRHAPMNKYCMGKS-NQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSR---RIESVNLLI-KIKTYLNGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDT-----------------SYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFS-LKGNLFHMFKN----ENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYS 5096
C + C+P GDLL+GR + L ASSTCGL+ +++YC+++ + + KC ICDS+ PY + +SH IENV VS P +WWQS +G+ +VSI + E+ F+F+ L FKT+RPAAM +ERS DYG W + YF+ +C FP+I + + +++C S++S I PS GG V+ D S + N ++ D+ +TN+R+ F +LHT GD + N +KYYYA+ E I+RG C C GHAS+C+ + G V MV+G C CQHNT G +CE+CKDF+ D+PW PA N CR C CNSHSS CHFD Y S +SGGVC++CQHNTE ++C+RC+ +YR+ I+ C+ C CD GT IC+ + LG AG C+CKENVEG +CD+CK + + + GC C+C +G+L CD TG+C C S+VTG C C +GY+ L N +++GCS C C G+ N C +G+C C+ G C E ++ + L +E + + P PV+ +G +F I + D + ++ G C QS + L +R +++P CLE D+ + V+ ++ + ++ + + +NS + +++ L E C+ A+ + + C+ L IS + + CKC+ GS ++C+++GGQCQC P V G+ C+RC++ SY GC C C GS ++ C TG+C C V G+ CD C GY+ FP CH C CN+ + CD +TG C NC G + G NC+ C +GYYG+ S G C+ C CP SS + + C ++ C C +GY+G C +C T +GN CQPC C+ NID +SC+ TGEC +CL NT G NC+ C G++G+ +TC +C C+ G + +C+ TG CPCLPNV GL CDRC D ++NL GC C+CD P + + C+QLTGQC C+ G GK C+ C++ ++GDP C C CN G+ C+ + G+CRC+ G++G+ CD C +G + P C C C+ QWDH + + L +N++ +E VC + L N+ ++ I K+ FP G + + ++ + + R I+ N Q ++ ++ D + +KN+ + E ++LN +SE+ K Y +I+ +K N + +D L ++ + ++ + I N +C + CGG C K +R C G + L + L I++ ++Q N +I+ + E N ++L + G I+++ E++NL I K+K +L L EN+ +I N ++ + I S+ N+ + L K + + + C+ ++ R E+ + LLN K TLN++ Q Q AN+ + L N+ + KN EN+ + ++ + +R + K+ + + K + + A+ + ++ + V K Y L+ +++ + + L K L D + + + I D E K+Q L+ K +L +L+D++ +
Sbjct: 23 CNRGACHPTTGDLLVGRNTQLMASSTCGLSRAQKYCILSYLEG--EQKCFICDSRFPYDPYDQPNSHTIENVIVSFEPDREKKWWQSENGLDHVSIRLDLEALFRFSHLILTFKTFRPAAMLVERSTDYGHNWKVFKYFAKDCATSFPNITSGQAQGVGDIVCDSKYSDIEPSTGGEVVLKVLDPSFEIENP--YSPYIQDLVTLTNLRINFTKLHTLGDALLGRRQNDSLDKYYYALYEMIVRGSCFCNGHASECRPMQKMRGDVFSPPGMVHGQCVCQHNTDGPNCERCKDFFQDAPWRPAADLQDNACRSCSCNSHSSRCHFDMTTYLASGGLSGGVCEDCQHNTEGQHCDRCRPLFYRDPLKTISDPYACIPCECDPDGTISGGICVSHSDPALGSVAGQCLCKENVEGAKCDQCKPNHYGLSATDPLGCQPCDCNPLGSLPFLTCDVDTGQCLCLSYVTGAHCEECTVGYWGLGN-HLHGCSPCDCDIGGAYSN-VCSPKNGQCECRPHVTGRSCSEPAPGYFFAPLNFYLYEAEEATTLQGLAPLGSETFGQSPAVHVVLGEPVPGNPVTWTGPGFARVLPGAGLRFAVNNIPFPVDFTIAIHYETQSAADWTVQIVVNPPGGSEHCIPKTLQSKPQSFALPAATRIMLLPTPICLEPDVQYSIDVYFSQPLQGESHAHSHVLVDSLGLIPQINSLENFCSKQDLDEYQLHNCVEIASAMGPQVLPGACERLI--ISMSAKLHDGAVACKCHPQGSVGSSCSRLGGQCQCKPLVVGRCCDRCSTGSYDLGHHGCHPCHCHPQGSKDTVCDQVTGQCPCHGEVSGRRCDRCLAGYFGFPSCHPCPCNRFAELCDPETGSCFNCGGFTTGRNCERCIDGYYGNPSSGQPCRPCLCPDDPSSNQYFAHSCYQNLWSSDVICNCLQGYTGTQCGECSTGFYGNPRISGAPCQPCACNNNIDVTDPESCSRVTGECLRCLHNTQGANCQLCKPGHYGSALNQTCRRCSCHASGVSPMECPPGGGACLCDPVTGACPCLPNVTGLACDRCADGYWNLVPGRGCQSCDCD-PRTSQSSHCDQLTGQCPCKLGYGGKRCSECQENYYGDPP--GRCIPCDCNRAGTQKPICDPDTGMCRCREGVSGQRCDRCARGHSQEFPTCLQCHLCFDQWDHTISSLSKAVQGLMR----LAANMEDKRETLPVCEADFKDLRGNVSEIERILKHPVFPSGKFLKVKDYHDSVRR--------QIMQLNEQLKAVYEFQDLKDTIER--AKNEADLLLEDLQEEIDLQSSVLNASIADSSENIKKYYHISSSAEKKINETSSTINTSANTRNDLLTILDTLTSKGNLSLERLKQIKIPDIQILNEKVCGDPGNVPCVPLPCGGALCTGRKGHRK------CRGPGCHGSLTLSTNALQKAQEAKSIIRNLDKQVRGLKN--QIESISEQAEVSKNNALQLRE-KLGNIRNQSDSEEENINLFIKKVKNFL-----------LEENVPPEDIEKVANGVLDIHLPIPSQ-----NLTDELVKIQKHMQLCEDYRTDENRLNEEADGAQKLLVKAKAAEKAANILLNLDK---TLNQLQQ------AQITQGRANSTITQLTANITKIKKNVLQAENQTREMKSELELAKQRSGLEDGLSLLQTKLQRHQDHAVNAKVQAESAQHQAGSLEKEFVELKKQYAILQRKTST--TGLTKETLGKVKQLKDAAEKLAGDTEAKIRRITDLERKIQDLNLSRQAKADQLRILEDQVVA 1744
BLAST of Netrin-4 vs. UniProt/SwissProt
Match: sp|P55268|LAMB2_HUMAN (Laminin subunit beta-2 OS=Homo sapiens OX=9606 GN=LAMB2 PE=1 SV=2) HSP 1 Score: 778.859 bits (2010), Expect = 0.000e+0 Identity = 461/1392 (33.12%), Postives = 709/1392 (50.93%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE-MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---------------------IEKPTYP-------------LEHLKEIKINLPPVSKSGYYNFVITTDTK-NMQFFDSFIIVLSIYHVGKSSCQSY----QDTMQ--LIKDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYAN-------------------LVSSK-----KCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKW-----QGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSS---------GVFMSNVKFPKEIDDV-----------CTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQ------QNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMD--NLINEMSKNVITFNGVTCNTITESNPLMC 3992
C + C P GDLL+GR L ASSTCGL + YC+V+ + KC +CDS+ P+ + HSHRI+NVV+ PQ WWQS +G+ V+I++ E+EF FT + FKT+RPAAM +ERS D+GRTW+ Y YFS +C DFP +P + ++V+C S +S I PS G V++ D Y ++ +N + ITN+RV RLHT GD +D IR KYYYA+ E ++RG C CYGHAS+C G E MV+G C C+HNT+G +CE+C+DFY D PW PA+ ++ CRKCEC+ H+ SCHFD VY S +SGGVCD CQHNT ++CE C+ +YR+ + C C CD +G++ C + LGL +G C CKE+V G +C +C+ GF+ + ++ GC +C C GT+ + CD ++G CYCK VTG C C G++ LS+ ++ GC C C G+ D + CD+ +G+C C+ G RC++++ ++ +E+ P L+ + ++ + V K+ Y+ ++ + + Q+ + +IV V S + D +Q L +RY++ PN CLE + L + + + P L+D R ++ E + + LV SK C L IS +T+ + + C+CN GS ++ CN GGQC C P V G+ C+ CA YGF GC+ C+C G+L+S C +G+CLCR G G CD C+ G W FP C C CN + C++ TG CL C+ ++ G +C+ C G++GD G C+ C CP G S ++ + C + C C+ GY+G C+ C HFG+ G CQ C CSGNID D+C+ TG+C +CL +T G +C C G+ G A ++C +C CN +GTN + Q C+ +G+CPCLPNV G CDRC F+NL+S GC PC C +P A +CN+ TGQC+C+AG G+ C+ C++ WGDP C C C+ G QC+R G C C+PG++G CD C +G G P C C C+ WD + + +L + G F S+ +E + S Q +++ +E+++ I +E L++L+ + + +FN + + + + + K + GA++ S+++ + ++E+++ L + R+ + + +D + ++ N+ + ++ + T ++T+ N L+C
Sbjct: 42 CSRGSCYPATGDLLVGRADRLTASSTCGLNGPQPYCIVSHLQD--EKKCFLCDSRRPFSARDNPHSHRIQNVVTSFAPQRRAAWWQSENGIPAVTIQLDLEAEFHFTHLIMTFKTFRPAAMLVERSADFGRTWHVYRYFSYDCGADFPGVPLAPPRHWDDVVCESRYSEIEPSTEGEVIYRVLDPAIPIPDPYSSRIQN-LLKITNLRVNLTRLHTLGDNLLDPRREIREKYYYALYELVVRGNCFCYGHASECAPAPGAPAHAEGMVHGACICKHNTRGLNCEQCQDFYRDLPWRPAEDGHSHACRKCECHGHTHSCHFDMAVYLASGNVSGGVCDGCQHNTAGRHCELCRPFFYRDPTKDLRDPAVCRSCDCDPMGSQDGGRCDSHDDPALGLVSGQCRCKEHVVGTRCQQCRDGFFGLSISDRLGCRRCQCNARGTVPGSTPCDPNSGSCYCKRLVTGRGCDRCLPGHWGLSH-DLLGCRPCDCDVGGALDPQ-CDEGTGQCHCRQHMVGRRCEQVQPGYFRPFLDHLIWEAEDTRGQVLDVVERLVTPGETPSWTGSGFVRLQEGQTLEFLVASVPKAMDYDLLLRLEPQVPEQWAELELIVQRPGPVPAHSLCGHLVPKDDRIQGTLQPHARYLIFPNPVCLEPGISYKLHLKLVRTGGSAQPETPYSGPGLLIDSLVLLPRVLVLEMFSGGDAAALERQATFERYQCHEEGLVPSKTSPSEACAPLL--ISLSTLIYNGALPCQCNPQGSLSSECNPHGGQCLCKPGVVGRRCDLCAPGYYGFGPTGCQACQCSHEGALSSLCEKTSGQCLCRTGAFGLRCDRCQRGQWGFPSCRPCVCNGHADECNTHTGACLGCRDHTGGEHCERCIAGFHGDPRLPYGGQCRPCPCPEGPGSQRHFATSCHQDEYSQQIVCHCRAGYTGLRCEACAPGHFGDPSRPGGRCQLCECSGNIDPMDPDACDPHTGQCLRCLHHTEGPHCAHCKPGFHGQAARQSCHRCTCNLLGTNPQQCPSPDQCHCDPSSGQCPCLPNVQGPSCDRCAPNFWNLTSGHGCQPCAC-HPSRARGPTCNEFTGQCHCRAGFGGRTCSECQELHWGDPGLQ--CHACDCDSRGIDTPQCHRFTGHCSCRPGVSGVRCDQCARGFSGIFPACHPCHACFGDWDRVVQDLAARTQRLEQRAQELQQTGVLGAFESSFWHMQEKLGIVQGIVGARNTSAASTAQLVEATEELRRE--------IGEATEHLTQLEADLTDVQDENFNANHALSGLERDRLALNLTLRQLDQHLDLLKHSNFLGAYD----SIRHAHSQSAEAERRANTSALAVPSPVSNSASARHRTEALMDAQKEDFNSKHMANQRALGKLSAHTHTL-SLTDINELVC 1410
BLAST of Netrin-4 vs. UniProt/SwissProt
Match: sp|P15800|LAMB2_RAT (Laminin subunit beta-2 OS=Rattus norvegicus OX=10116 GN=Lamb2 PE=1 SV=1) HSP 1 Score: 771.155 bits (1990), Expect = 0.000e+0 Identity = 435/1185 (36.71%), Postives = 630/1185 (53.16%), Query Frame = 3
Query: 96 ARYILEIESDNV--CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE-MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEW---YIEKPTYP--------LEHLKEIKINLPPVSKSGY----------YNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQS-------YQDTMQ--LIKDSRYIVIPNK-CLEKDLP------------------------IVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGI-----CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFL 3410
A + ++ S +V C + C P GDLL+GR L ASSTCGL + + YC+V+ + KC +CDS+ P+ + +SHRI+NVV+ PQ WWQS +GV V+I++ E+EF FT + FKT+RPAAM +ERS D+GRTW Y YFS +C DFP IP ++V+C S +S I PS G V++ D Y ++ +N + ITN+RV RLHT GD +D IR KYYYA+ E +IRG C CYGHAS+C G E MV+G C C+HNT+G +CE+C+DFY D PW PA+ T+ CRKCECN HS SCHFD VY S +SGGVCD CQHNT ++CE C+ +YR+ + C C CD +G++ C + LGL +G C CKE+V G +C +C+ GF+ + + GC +C C GT+ CDSS+G C+CK VTG+ C C G++ LS+ ++ GC C C G+ D + CD+ +G+C C+ G RC++++ + +++ T+ LE ++ + N S +G F++T+ + M +D + + L + G S S D +Q L ++R +V P CLE L I++ ++ + + L + + + E L + S+ C L IS +++ + + C+C+ GS ++ CN GGQC+C P V G+ C+ CA+ YGF GC+ C+C G+L++ C +G+CLCR G G CD C+ G W FP C C CN + CD+ TG CL C+ + G +C+ C G++GD G C+ C CP G S ++ + C + C C+ GY+G C+ C HFG+ G CQ C CSGNID +C+ TG+C +CL +T G +C C G+ G A ++C +C CN +GT+ + C+ TG+CPCLP+V GL CDRC F+N +S GC PC C +P A +CN+ TGQC+C AG G+ C+ C++ WGDP C C C+ G QC+R+ G C C+PG++G CD C +G G P C C C+ WD +
Sbjct: 31 AATLAQVPSLDVPGCSRGSCYPATGDLLVGRADRLTASSTCGLHSPQPYCIVSHLQD--EKKCFLCDSRRPFSARDNPNSHRIQNVVTSFAPQRRTAWWQSENGVPMVTIQLDLEAEFHFTHLIMTFKTFRPAAMLVERSADFGRTWRVYRYFSYDCGADFPGIPLAPPRRWDDVVCESRYSEIEPSTEGEVIYRVLDPAIPIPDPYSSRIQN-LLKITNLRVNLTRLHTLGDNLLDPRREIREKYYYALYELVIRGNCFCYGHASQCAPAPGAPAHAEGMVHGACICKHNTRGLNCEQCQDFYQDLPWHPAEDGHTHACRKCECNGHSHSCHFDMAVYLASGNVSGGVCDGCQHNTAGRHCELCRPFFYRDPTKDMRDPAACRPCDCDPMGSQDGGRCDSHDDPVLGLVSGQCRCKEHVVGTRCQQCRDGFFGLSASNPRGCQRCQCNSRGTVPGGTPCDSSSGTCFCKRLVTGDGCDRCLPGHWGLSH-DLLGCRPCDCDVGGALDPQ-CDEATGQCPCRPHMIGRRCEQVQPGYFRPFLDHLTWEAEGAHGQVLEVVERLVTNRETPSWTGVGFVRLREGQEVEFLVTSLPRAMD-YDLLLRWEPQVPEQWAELELVVQRPGPVSAHSPCGHVLPRDDRIQGMLHPNTRVLVFPRPVCLEPGLSYKLKLKLTGTGGRAHPETPYSGSGILIDSLVLQPHVLMLEMFSGGDAAALERRTTFERYRCHEEGLMPSKTPLSEACVPLL--ISASSLVYNGALPCQCDPQGSLSSECNPHGGQCRCKPGVVGRRCDACATGYYGFGPAGCQACQCSPDGALSALCEGTSGQCLCRTGAFGLRCDHCQRGQWGFPNCRPCVCNGRADECDAHTGACLGCRDYTGGEHCERCIAGFHGDPRLPYGGQCRPCPCPEGPGSQRHFATSCHRDGYSQQIVCHCRAGYTGLRCEACAPGHFGDPSKPGGRCQLCECSGNIDPTDPGACDPHTGQCLRCLHHTEGPHCGHCKPGFHGQAARQSCHRCTCNLLGTDPQRCPSTDLCHCDPSTGQCPCLPHVQGLSCDRCAPNFWNFTSGRGCQPCAC-HPSRARGPTCNEFTGQCHCHAGFGGRTCSECQELHWGDPGLQ--CRACDCDPRGIDKPQCHRSTGHCSCRPGVSGVRCDQCARGFSGVFPACHPCHACFGDWDRVV 1204
BLAST of Netrin-4 vs. TrEMBL
Match: A0A0X3PV22 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR117450 PE=4 SV=1) HSP 1 Score: 1019.22 bits (2634), Expect = 0.000e+0 Identity = 542/1383 (39.19%), Postives = 785/1383 (56.76%), Query Frame = 3
Query: 48 RELI*MTLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLK-EIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSS------------KKCD--FLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFL--------------------------IVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLK-------EIKKYFPIGYDIIISNFSEKLSRL-----KYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISK---NKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKP 4025
R+LI L + +F++++ L E + C TIC+P+AGDLLIGRES L+ASSTCGL + + YCVVT +G KS C C SK Y + ++ +SHRIENV+SR+P DP RWWQS + VHNVSI I+ E++FQF T +RFKT+RPAAMY+ERS ++G+TW YAYFS+NCPRD+P IPER R +N+V CT+ +S + PSEGG V F A S+ N ++++R +++AITN+R+ F RL+T GD ++DD+ I+ KYYYAI EW + GRCSC+GHA++C+ K +++ E VYGVC+C HNT G +CE+C DFY PW PA + S N C +CECN HS+ C+F+P++Y QS +SGG C CQHNTE +CE C G+YR +I+ QTC C+C G+ P C Q + AG C CKENV G++CD+C++G+W F + GC C C M+GT EN GCD TGKC CK V G C TC+ G+FNL+++ + GC C CS +GS CDK +G+C CK GF G C +++ +YI P ++ + + +++ SK G Y V+ D K D + + L + S C + + +++ +D+ IV+P CLE +T+F + P + +L + T +V C + +L+ ++C+ F +I N T I C+CNVTGS + C+K+ GQC C P V G+TC +C Y F A GC C C+ GSLN QC T +C CREG+EG+ CD C+ GYW+FP+C C+CN MS TCD TG+CL C+ N+ G C+ C GYYGD RG+ C+ C+CPG ++S GC+L C CK GY+G C +C N+FGN GTC+PC CSGN+ E CN TGEC CL NT G C++C G++GN +++ C +C C H+G C+ TG+C CLPNV+G +C C FNL+S +GC C CD PIG+L C+ ++G C C+ RSG+ C+ C+ ++GDP GC C C+ GS+ C+ +G C+C+PGI+GR CD C +GT G +PNC CG+CW WD + + N +++ G ++N K E D + LE+ +K+L E+ K + D++ + + L K + + D + I ++ YA + ++ +++ D + + + SL+ + A ES K +EE R + K + ++ KD+ I ++++ + NG + ++ C CGG C P
Sbjct: 3 RQLI---LRLLISVFISKFALAYEEEYSCENTICSPKAGDLLIGRESALQASSTCGLNDVQPYCVVT---AGGKSHCKECYSKQEYNQITNPNSHRIENVISRVPSDPDRWWQSENAVHNVSIVINLETQFQFISTIMRFKTFRPAAMYLERSDNFGQTWKPYAYFSNNCPRDYPGIPERPRRTLNDVTCTNVYSQLTPSEGGVVAFMAAPSEMHANLPLFSEERQNLSAITNLRITFSRLNTLGDMELDDSSAIKRKYYYAIYEWNVWGRCSCFGHATRCRPKSPHELENMEKVYGVCECTHNTAGDNCERCADFYQAKPWRPATRESANACERCECNGHSNQCYFNPLLYLQSNNVSGGQCHACQHNTEGIHCEFCADGFYRYPGYSIDHPQTCQPCNCHGDGSIDPDKCQQYTDPQQNTVAGRCNCKENVAGDKCDRCRVGYWNFQASNPKGCEACRCHMLGTWENSGCDPMTGKCTCKRHVGGEYCDTCQPGFFNLTDSPL-GCQPCACSPFGSL-GAECDKKTGRCHCKQGFVGRDCGQVEDGYYIRPPELMIDQPRIPVDVSIESPSKDGSYVIVLAIDPKQFGPGDRWTVTLEPKQL-DSRCINRKYIVEITRDTSKIVLPEVCLEAGKKTFVTIFA--DPRQFSPYTSVLFADIILIPDEGDTGRVAEYCEQFRHLIMQIAQGEVDPARMPRQCEEYFRLPQIHWNE-TGMVTIPCECNVTGSVSAVCDKMTGQCLCKPGVVGRTCAQCEPFHYHFSASGCTACDCNAQGSLNRQCDPSTSQCPCREGIEGRQCDRCRSGYWDFPYCRKCECNGMSDTCDQLTGRCLACRDNTGGDRCETCAQGYYGDPLRGVPCRPCECPGGGV--DHSEGCNL-HPVAGVVCRCKPGYAGPRCSECAPNYFGNPTELGGTCKPCECSGNLPP--EGGCNQVTGECLVCLHNTEGPRCDQCKLGFWGNASLQLCQRCSCYHLGAVGGADAACDRTTGECNCLPNVLGPRCFECVPYHFNLTSGVGCESCACD-PIGSLFPECHPISGACTCKPDRSGRKCDQCQRTYFGDPRDGGGCKPCDCDRSGSLSDVCDPASGQCQCRPGISGRRCDRCDRGTTGTLPNCRPCGECWTNWDKAIDRVIVEMRRLENQTSNQLPPDLKPVFTAISNLVEQIELLPGAKLTNEKL-NEFDGLLALLEKKVKTLDNLDTQTGELSKAIAV-LDMLTKEQTSITNTLSGSEKKLEALSVLLDDMKNRSAQIAILDPHGAYAALLAAREEVSRLDDTQRSLQKRRTSLEAMRQSAMESVS----------KREAADEEFRKLT-KQLEERIKDLQEEIRQLNRLICGDNGQVPD--GSADRATCPSACGGTSCDP 1352
BLAST of Netrin-4 vs. TrEMBL
Match: A0A4S2L4E2 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009869 PE=4 SV=1) HSP 1 Score: 1014.22 bits (2621), Expect = 0.000e+0 Identity = 525/1305 (40.23%), Postives = 757/1305 (58.01%), Query Frame = 3
Query: 78 VFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVK-EMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRK-----------VLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESK-KIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSK-NVITFNG 3950
VF +F+ + C TICNPRAGDLLIGRES L ASSTCGL E YCVVTTS +S C C S PY ++ SHRI+NVVSR+P +P RWWQS + VHNVSI ++ +++FQF T LRFKTYRPAAMY+ERS D+GRTW KYAYFS+NC RDFP++PE R ++ +V CT +S + PSEGG V + A S+ + ++K+R ++TAITN+R F RL+T GD VDD+ IR KYYYA+ EW + GRC+C+GHA +CK K +K E VYGVC+C H+T G++CE C+DFY + PW PA K+S N C KCECN H+++C F+P++Y S +SGG C C HNTE NCE C+ +YR+ IN S TC C+C + G + C Q + AG C+CK+NV G +CD+CK+G+W F + GC C C MMGTL+N GCD TG C CK FV G +C C GYFNL+ A + GC C CS+ G++ N C++ +G+C CK GF G C +++ +Y+ PT ++ E +I + G + V+ D K D + I++ Y G + CQ+ T+ + +D+ I+I + CLE LP+VL + + I+ ++ + +S R+ + G + L + F +S T S +C CN T S + C+K+ GQC C P V G+ C+RCA Y F +RGCK C C+ GS++ QC +T C C EG+ G+HCDTCKPG WNFP C C CN+MS C+ TG C NC+ N+ G C+ C+ G+YGD RG CK C CPG N++ C + C C+ GY+G CD+C N+FGN G+C+PC+CSGNI C+ +TG C KCL N+ GFNC+ C GY+G+ + C C C G+ + + CN + G+CPCLPNVVG +C C FFNL+S GC PC CD P G+++ C+ +TGQC C+ RSG+ C+ C+ F+G P +L+GC C C+ G++ C+ G C C+ G+ GR CD C +GT+G++PNC CG+CW WD + V D+L N +G + P +++ + L ++ ++++ + I D + + L+R + + + + + ++ + ++D + M N++ + EL + L +IL A +++ ++ + L + +++R+I Q++ DNL ++ N I G
Sbjct: 9 VFGVFLGNIFRSSSEEYSCENTICNPRAGDLLIGRESSLWASSTCGLNEVEAYCVVTTSG---RSFCKECTSSQPYNALTNPESHRIDNVVSRVPSNPGRWWQSENAVHNVSIVLNLDTQFQFISTILRFKTYRPAAMYLERSYDFGRTWKKYAYFSNNCKRDFPAVPEGSRRSLTDVTCTGVYSQLTPSEGGVVAYMAAPSEMHESWPQYSKERMNLTAITNLRAVFTRLNTLGDMNVDDSSLIRRKYYYALYEWNVWGRCTCFGHAKQCKPKSTAEIIKPEKVYGVCECTHHTAGENCETCEDFYWNKPWRPATKDSPNACEKCECNGHATACFFNPILYASSGNVSGGNCHGCMHNTEGVNCEYCQQNFYRHPDYPINHSMTCQPCNCHTDGALYAGFCQQYTIPEQNSVAGRCLCKKNVGGEKCDRCKVGYWNFQASNPEGCEPCTCNMMGTLDNGGCDPYTGLCTCKRFVGGPNCDQCLEGYFNLTLAPL-GCQECACSQLGAK-NPNCNRDTGQCECKVGFTGRDCAQVEDGYYVVPPTEIIDGRAEKEITVDGPKGEGRFVIVLDVDPKQFGEGDQWTIIVKPYQHGATRCQNPSQTVNVYQDTSKIIISDVCLEAGLPLVLRIMPESQPSGPYTEILITDIVLVPTDDSGLARRCEVYRELAMDILSGRTEDRRGLPPECEVYFRLPRVSWRE-TGSVAERCLCNTTWSETDICDKMTGQCVCKPGVVGRRCDRCAPYHYEFGSRGCKDCACNPQGSVSLQCDPETTLCRCHEGIVGKHCDTCKPGTWNFPRCQPCFCNEMSNMCNQTTGHCENCRENTGGMYCEKCKEGFYGDPQRGTPCKPCACPGGGV--NHATECRM-GPLEEQICYCQVGYTGARCDECAMNYFGNPTELGGSCRPCQCSGNIALNEPGGCDPQTGRCLKCLHNSEGFNCDVCKLGYWGDATRQMCQPCNCYAPGSVDEAKAGCNRDDGQCPCLPNVVGARCYECAPGFFNLTSGKGCEPCQCD-PTGSVSDKCDAITGQCQCKPDRSGRKCDRCRPTFYGSPNELDGCKPCNCDRSGAISDFCDSVTGQCPCRTGVAGRRCDRCDRGTEGQLPNCLPCGECWTNWDKAIDKVTAELDRLRNQTG-----RQLPSDLEVLFNGLVDLLQQIEKV-PWMNI-EDEKMEELQKILNRTNSALQELSSFEETPESLNALSEKLNSDLHEEMNRANEVAKLHMKMTELTERLDSILEEAKKAETQLPEGAYLAVQRAIEIEKQVRSIKETIEGQRALMEDNLSKAKAEVNAIKLRG 1296
BLAST of Netrin-4 vs. TrEMBL
Match: G4LWP4 (Laminin beta chain-related OS=Schistosoma mansoni OX=6183 GN=Smp_148790 PE=4 SV=1) HSP 1 Score: 1006.9 bits (2602), Expect = 0.000e+0 Identity = 594/1749 (33.96%), Postives = 901/1749 (51.52%), Query Frame = 3
Query: 81 FIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVK-EMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRK-----------VLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCP--GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIEN--STDYAKAMISKNKIRDVTGAFNELKKSLKNILNGAS-----ESKKIYQNITLIKD---------------------KLFYRNEELRNINLKFIRQKS--KDMDNLINEMSKNVITFNGVTC-----NTITESNPLMCSEFCGGVGC--------------------------------------KPDKCYRHAPMNKYC---MGKSNQFFNLY-ASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAI---QSRRIESVNLLIKIKTYLNGSADLKDFESLIENINKFEINF--KENEIIALDKQIRSR-IRKLNNVNNILDKSKFNRKQCDVFKKS-AVRAKEDTSYLLNSIKNISTLNRMTQKFEII--MKQFVMNF----------ANALFSLKGNLFHMFK-NENEAFQKQNNISYVTERIKFLKLYIMKIHKVFS--NTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLK 4988
+++ + Y + D C TICNPRAGDLLIGRES L ASSTCGL E YCVVTTS KS C C SK PY ++ SHR+ENVVSR+P +P RWWQS + VHNVSI ++ +++FQF T LRFKTYRPAAMY+ERS D+GRTW +YAYFS+NC RDFP++PE R ++ +V CT +S + PSEGG V + A S+ + ++K+R ++TAITN+R F R++T GD +VDD+P R KYYYA+ EW + GRC+C+GHA +CK K +K E VYGVC+C HNT G++CE C DF+ + PW+PA +++ N C KC CN+H+++C F+PV++ +S +SGG C C HNTE NCE C+ +YR+ + I+ TC C+C GT + + C Q + AG CICK+NV G +CD+CK+GFW F GC C+C M+GT++N GCD TG C CK FV G +C C GYFNLS + GC C CS+ GS N C++ SG+C CK GF G C E+++ +YI P ++ E +I L G Y V+ D K D + IV++ + G + CQ+ ++ + +D+ ++I + CLE LPIVL + I+ +I + +S R+ + G + L + F +S + S +C CN TGSR+ C+K+ GQC C V G+ C+RCA YGF ARGC C CD GS++ QC+ DT C CR G+ G+HC+ C PG+W+FP C C CN+ S C+ TG C NC+ N+ G +C+ C G+YGD R CK C CP GL+ K +G C+CK GY+G C QC N+FGN G+C+ C CSGNI + SC+ TG+C KCL NT G+ C+ C G++G+ + C C C +G+ + G CN E G+C CLPNV+G +C C FFNL+S GC PC CD P G+++ C+ + GQC C+ RSG+ C+ C+ F+ DPT + C C C+ GS C+ G C C+ GITG+ CD C +GT+G +P+C CG+CW WD + V + D+L N ++ + PKE+ + SL + ++ + + D ++ + L R + + + +D +S++ ++ + A+ + K + + LK +++I++ + + + Y +I KD +L EL + + + S +D I E + + N + C + +++ +C CGG GC + + M +Y + +N +N+Y A + + N S + E N++I + +IK+ YN T LI+ + S R + + ++K + L + E +I+ + K+ I+ K I+A + R R + L I DK+K +Q + K++ + + ++ + ++ +++TQ E I K V+ F A F + +L E I V K L ++++ K+ +T + ++ L + + + N ++ +L + I + L +S L D++K K++
Sbjct: 13 YVLTTSSYEIAFSEDYSCENTICNPRAGDLLIGRESSLVASSTCGLNEVEAYCVVTTSG---KSYCKECTSKQPYNSITNPESHRVENVVSRVPSNPGRWWQSQNAVHNVSIVLNLDTQFQFISTILRFKTYRPAAMYLERSYDFGRTWARYAYFSNNCKRDFPAVPEGPRRSLTDVTCTGVYSQLTPSEGGVVAYMAAPSEMHQSWPQYSKERMNLTAITNLRAVFTRINTLGDMRVDDSPLTRRKYYYALYEWNVWGRCTCFGHALRCKPKSSAEIIKPEKVYGVCECTHNTAGENCETCADFHWNKPWMPATRDAANACEKCNCNNHATACFFNPVLFSKSGNVSGGNCHGCIHNTEGVNCEFCQPNFYRHPSYPIDHPLTCQPCNCHLDGTLYDNFCQQYTIPEQNTIAGRCICKKNVGGEKCDRCKVGFWNFQAENPDGCEACSCNMIGTIDNGGCDPFTGLCICKRFVGGPNCDRCLEGYFNLSTTPL-GCQECACSQIGSL-NPNCERVSGQCACKVGFTGRDCSEVENGYYIVPPHEIIDRPDEKEITLIGPKGEGKYVIVLDVDPKQFGSGDRWSIVVTPFQYGATKCQNPPQSVNIYQDTHKVIISDVCLEPGLPIVLRIVPESYPEGAFTEILITDIILIPTDDSELARRCEVYREIAMEILAGRREDRTGLPPECEVYFRLPRLSWKE-SSSVAQRCLCNSTGSRSEICDKLTGQCPCKDGVVGRQCDRCAPYHYGFSARGCTDCACDPQGSVSLQCHEDTSLCECRPGIVGKHCNMCNPGFWDFPNCRPCICNEFSDFCNQTTGICENCRDNTGGDHCETCREGFYGDPLRKTPCKPCSCPGGGLNHAKECHMG-----PLEEQICICKVGYTGPRCSQCAMNYFGNPTEPGGSCRQCECSGNIAVNVPGSCDPITGQCLKCLHNTEGYYCDVCKDGFWGDATRQMCQPCNCYPLGSIDEGAGGCNRENGQCSCLPNVIGPRCYECAPYFFNLTSGKGCEPCLCD-PTGSIDDLCDPVMGQCRCKPDRSGRRCDQCRRLFYSDPTTPDRCLPCDCDRVGSTSDLCDPLTGQCPCRTGITGQRCDRCDRGTEGILPDCVPCGECWNNWDKAIDKVISELDRLQN-----QTSQQLPKELKIMFDSLVSLLDQIEHVP--WMTTDDTRLNELRDVLKRSEETIMRLSSLD--QFADSLVKWDDLITERLAQQLERKGVVNEAKARLETLKTQMQSIMDESMKLEHLDKRGAYLSILRSKDIGDRVINTQTSIEYQRGGLEAELLNSKRELEAVQTRADQLNSDWAQLDRRIEEFREKIRQTNRLICGDNGLSPTEDTDQKVCPSTCGGAGCDYFFSKSASLNTISNTNILMNNLNTGLISTCGVNPGCNASNAGRLRSRMEEYLNLQIRLTNALYNVYRAENAISKVNSSRRIAE-QTITNLTIQATDIKERIA---DMYNLTNHLINEAEKYVNLYSSMRQDKFDEILKSLQNAKLNVSLYEAEQIIQQLEKYAIDLEDKAKRILAETESDRQRSVNLLRRAETIRDKAKDFMEQINQLKEADKIYQGIEKEPIIQKVDDVELFDQITQLTEQIDGFKNQVIMFSRDSSLQSDKAKEAFEVADDLLSSTNVARRETLDTLQQIELVEVSFKQLDEKMLELDKLMRALDTGDRGTGDEDLPDTTLTDPAKLMEEIQNYTSTLELNTDEVKSIITNLKRLEIESELLTDELKGYMDKMQ 1736
BLAST of Netrin-4 vs. TrEMBL
Match: A0A4Z2DB14 (Laminin subunit beta-2 OS=Schistosoma japonicum OX=6182 GN=EWB00_002741 PE=4 SV=1) HSP 1 Score: 1004.59 bits (2596), Expect = 0.000e+0 Identity = 570/1596 (35.71%), Postives = 857/1596 (53.70%), Query Frame = 3
Query: 78 VFIIFVARYILEIES-------DNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCD----------FLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCP--GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIEN--STDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASE---------------SKKIYQNITLIKDKLFYRN-----------EELRNINLKF--IRQKSKDMDNLINEMSKNVITFNGVTCN-----TITESNPLMCSEFCGGVGC----KP-------------DKCYRHAPMNKYCM-GKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISI-------NSKEIKDAYVKPESEYNTTIK-LIDVTRGAIQSR-RIESV-----NLLIKIKTYLNGSADLKD--FESLIENINKFEINFKENEIIALDKQIRSRIRKLNN-VNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKF 4595
+ +IF +I + S D C TICNPRAGDLLIGRES L ASSTCGL E YCVVTTS KS C C SK PY ++ SHR+ENVVSR+P DP RWWQS + VHNVSI ++ +++FQF T LRFKTYRPAAMY+ERS D+GRTW +YAYFS+NC RDFPS+PE R ++ +V CT +S + PSEGG V + A S+ + ++K+R ++TAITN+R F R++T GD +VDD+P R KYYYA+ EW + GRC+C+GHAS+CK K ++ E VYGVC+C HNT G++CE C DF+ + PW+PA +++ N C KC CN+H+++C F+PV+Y +S +SGG C C HNTE NCE C+ +YR+ + I+ TC C+C GT + + C Q + AG CICK+NV G +CD+CK+GFW F GC C+C M+GT++N GCD TG C CK FV G +C C GYFNLS A + GC C CS+ GS N C++ SG+C CK GF G C E+++ +Y+ P + E +I+L G Y V+ D K D + IV++ + G ++CQ+ ++ + +D+ ++I + CLE LPIVL + I+ +I + +S R+ +++ ++ D + + + S +C CN TGSR + C+K+ GQC C V G+ C++CA YGF ARGC C CD GS++ QC+ T C CR G+ G+HC+ C PG+W+FP C C CN +S C+ TG C NC+ N+ G +C+ C G+YGD R CK C CP GL+ K +G C CK GY+G C QC N+FGN G+C+PC CSGNI + SC+S TG+C KCL+NT G+ C+ C G++G+ + C C C +G+ ++ G CN + G+C CLPNV+G +C C FFNL+S GC PC CD P G+++ C+ + GQC C+ RSG+ C+ C+ F+ DPT +GC C C+ GS C+ G C C+ G+TG+ CD C +GT+G +PNC+ CG+CW WD + V D+L N ++ + PKE+ + SL + ++ + + I D ++ F L R + V + +D +S+I ++ + + + K + + LK LK+I++ + + SK+I + I+ + Y+ EL + + + ++ I E + + N + C +S+ +C CGG GC KP D+ K M G++ +L + +N + + N+ I N ++A K S + ++D+T A + RI + NL+ + + Y+N + ++ F+ ++ + + ++N E + +Q+ L + IL +++ +R++ + A ++ + I + +++ Q+
Sbjct: 5 LLVIFTVSHISTLASYETAFTEDYFCENTICNPRAGDLLIGRESSLIASSTCGLNGIEAYCVVTTSG---KSHCEECISKQPYNPEANPESHRVENVVSRVPSDPGRWWQSQNAVHNVSIVLNLDTQFQFISTILRFKTYRPAAMYLERSYDFGRTWARYAYFSNNCKRDFPSVPEGPRRSLTDVTCTGVYSQLTPSEGGVVAYMAAPSEMHESWPQYSKERMNLTAITNLRAVFTRINTLGDMRVDDSPLTRRKYYYALYEWNVWGRCTCFGHASRCKPKSSVEILKPEKVYGVCECTHNTAGENCETCADFHWNKPWMPATRDAANACEKCNCNNHATACFFNPVLYIKSGNVSGGNCHGCMHNTEGVNCEFCQPNFYRHPSYPIDHPLTCQPCNCHLDGTLYNNFCQQYTIPEQNTIAGRCICKKNVGGEKCDRCKVGFWNFQAENPDGCESCSCNMIGTIDNGGCDPFTGLCTCKRFVGGPNCDRCLEGYFNLSTAPL-GCQECACSQIGSL-NPNCERVSGQCACKVGFTGRDCSEVENGYYVVPPHEIISGSDEKEISLIGPKGEGKYVIVLDVDPKQFGSGDRWSIVITPFQYGATTCQNPPQSVNIYQDTHKVIISDVCLEPGLPIVLRIVPESYPEGAFTEILITDIILIPSDDSDLARRCESYREIAMEILAGRREDRTGIPPECEVYFRLPRLSWRESSSVAQRCLCNSTGSRTDICDKLTGQCPCKDGVVGRQCDQCAPHHYGFSARGCTDCACDPQGSISLQCHETTSLCECRPGIVGKHCNMCNPGFWDFPNCRPCTCNGLSEFCNQTTGICENCRDNTGGDHCETCREGFYGDPLRKTPCKPCSCPGGGLNHAKECRMG-----PLEEQICTCKVGYTGPRCSQCAVNYFGNPTEPGGSCRPCECSGNIAVNVPGSCDSVTGQCLKCLYNTEGYYCDICKEGFWGDATRQMCQPCNCYPLGSVNEGAGGCNRDNGQCSCLPNVIGPRCYECAPYFFNLTSGKGCEPCLCD-PTGSIDDLCDPVMGQCRCKPDRSGRKCDQCRRMFYSDPTSPDGCRPCNCDRAGSTSDLCDALTGQCPCRTGVTGQRCDRCDRGTEGILPNCNPCGECWNNWDKSIDKVIIELDRLQN-----QTSQQLPKELKILFDSLVSLLDQIEHLP--WMITEDARLNEFRNVLKRSEDIVMRLSSLD--QFADSLIKYDDLMTERLTQQLERKVLVNEAKTRLESLKNQLKSIMDESMKLEHLDQKGAYLSLLRSKEIADRVADIQTSIEYQRGGFQTELSDSKRELEAVQTRADQLTLDWAQLERRIEEFREKIRQTNRLICGDNSSPPTEDSDQKVCPSTCGGAGCDYFSKPTSLSTISSLSVGNDRNPSSTATTKEMMNGRTGLISTCGLNLGCNASNAGRLRSRMEEYLNLQIRLTNALHNVYRAENAISKVHSSRKIAEETVMDLTLNATNIKERIADMYNLTQNLINEAEKYVNIYSSMRQDKFDEILRTLQESKLNVSRQEAEQIIQQLEKYATDLEDKAIRILAETESDRQRTGNLLRRAETIRDKAKDFMEQINQLKEADKIYQEI 1580
BLAST of Netrin-4 vs. TrEMBL
Match: A0A0R3T2Q6 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS1240 PE=4 SV=1) HSP 1 Score: 1001.5 bits (2588), Expect = 0.000e+0 Identity = 568/1588 (35.77%), Postives = 857/1588 (53.97%), Query Frame = 3
Query: 132 CRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLK---EIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTK-----------------SDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNT---------------QQNSI-ITIENSTDYAKA-------MISKNK---IRDVTGAFNELKKSLKN-----ILNGASESKK-----IYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLM--CSEFCGGVGCKP--------DKCYRHAPMNKY----------------CMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKE----IKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESV-NLLIK-----IKTYLNGSAD-LKDFESLIENINKFEINFK-----ENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFE 4598
C+ TIC+PRAGDLLIGR++ L ASSTCGL N + YCVVT S KS C+ C SK Y ++ +SHRIENV+SR+P DP RWWQS + + NV++ ++ E++FQF T LRFKT+RPAAMY+ERS D+ +TW YAYFS+NCPRDFP+IPER R +++V CTS +S + PSEGG V F A S+ N ++++R ++TAITN+RV F RL+T GD +D++ IR KYYYA+ EW + GRCSC+GHA++CK K ++ E VYGVC+C HNT G +CE+C FY + PW PA ++S N C +CEC+ HS C+F+P++Y QS +SGG C C HNTE CE C GYYR+ IN QTC C+C + G++ C Q G AG C+CK+NV G++CD+CK+G+W F GC +C C M+GTLEN GCD TG C CK FV G +C C GYFNL+++++ GC C C+ GS CD G C CK F+G C ++ +YI +P + +I I +P +G Y VI TD K+ FD + I + I SSC++ +++ ++R IVIPN CL D + + + ++ P + ++ + + + C +Y + L+ EI N + + + + C+CNVTGS C+K+ GQC C P V G+ C++C + Y F + GC C C GS QC T +C CR+G+EG+ CD C GYWNFP+C C+CN + CD TG C++C+ N+ G C+NC G+YGD RG+ C+ C+CPG ++S GC+ + C C+ GY+G C +C N++GN G+CQPC CSGN+ E C+ TG C+ CL+NT G C++C++G++GN + +TC C C +GT +C+ ETG C CLPNV+G +C C + FNL+S GC CNCD P G+++ +C+ LTGQC C+ RSG+ C+ C+ F+GDP +GC +C C+ GS+ C+ G C C+PGI GR CD C +GT G +P+C CG+CW WD + V + +L N ++ + P ++ V L+ ++ L+ I Y + F E L ++ V D NT QQN + T+ ++ Y K M+ ++ I+D GA++ L + ++ L + ES+K + Q+ + +EE + + +KF+ ++ KD+ + I ++++ + N + ES + C CGG C P D+ + N M + L A L + ++ +L+ Q N+ I+S + + + + K ++ RG + R++++ N +I+ +K Y N +AD + S I +N +N N + L Q+R ++ N ++ +++ D+ + A +E L + +S ++++ + E
Sbjct: 35 CQNTICSPRAGDLLIGRDNALVASSTCGLENVDTYCVVTASG---KSHCSECYSKQAYNSITNPNSHRIENVISRVPSDPGRWWQSENALRNVTLTLNLETQFQFISTILRFKTFRPAAMYLERSVDFAQTWQPYAYFSNNCPRDFPAIPERPRRALSDVTCTSIYSQLTPSEGGVVAFMAAPSEMHANLPPYSEERQNLTAITNLRVTFTRLNTLGDMNLDNSSAIRRKYYYAMYEWNVWGRCSCFGHATRCKPKSASEIPNLEKVYGVCECTHNTAGDNCERCASFYQNKPWRPASRHSANACERCECHDHSDQCYFNPILYLQSGNVSGGHCYQCHHNTEGPQCEFCSVGYYRHPDYPINHPQTCQPCNCHAGGSETHESCDQYTNPVEGKIAGRCVCKKNVGGDKCDRCKVGYWNFRAENPDGCEECKCHMLGTLENMGCDQITGACTCKRFVEGENCDRCMPGYFNLTSSSM-GCQPCACNPVGSL-GPECDIERGICKCKANFSGRDCSQVDDGYYIRRPQIVVNQPGTTIDIPIEVP--KNTGNYIVVIATDPKHFSRFDRWSITVDIRQ-DDSSCRNSPTHLEITAETRKIVIPNVCLTGDRKTFIAI--MPTSRYGSPYSEITIGNIIVKQDDGEASAIAEYCESYREIYER----ILDGEIEPNRLPQRCQTYFNIQESQWKDDSAISVPCECNVTGSVTPICDKLNGQCPCRPGVVGRKCDQCEAYHYHFSSSGCTACNCHPKGSTGLQCDPLTSQCPCRQGIEGRKCDICGSGYWNFPYCQRCECNGKADECDQVTGACIDCRDNTGGARCENCAQGFYGDPFRGVPCRPCECPGGGV--DHSEGCT-VHPVAGMQCHCQMGYTGPRCSECAPNYYGNPTEYGGSCQPCDCSGNLPP--EGGCDQVTGACQVCLYNTEGEKCDRCIAGFWGNASQQTCQPCSCYQLGTAGGGSAVCDRETGACNCLPNVLGPRCFECAPQHFNLTSGKGCESCNCD-PTGSIHLNCHPLTGQCTCRPDRSGRKCDQCQRTFYGDPRTPDGCKRCDCDRAGSLSDTCDPITGQCVCRPGIAGRRCDRCDRGTKGTLPHCEPCGECWTNWDKAITAVVDEMGRLKN-----QTSSQLPADLKPVFDILQGLVEQLELIPASNLTDYQ--LKKFEEVLLFIEEQVSGLESPDGNTGKLPEVEEALNKLQEQQNKLNQTVGDNEKYLKQISDELEKMVESSRNISIQDPFGAYDALLMAQRDAQKIEALQKSLESRKATLEALLQSTSTSVASRSEADEEFKRL-IKFLSERIKDLQDEIRQLNRMICGDN----QRVPESGADLRTCPSTCGGASCDPITAEIFDLDEQRGTSTPNDLPNFFGLISSCAGTIGCSMSSEGRLKELLAEHLTAQNALANVLQNAAQVENL-IDSASPNFCVNATRTEADEIMTSLFKELETLRGRV--FRVQNITNAIIEEGQEAVKKYENITADYYNELVSKIRGLN-HAVNIDGALRLGNTLEKLSAQLRDKVEHFNT------ETSGQKREADLLLERAKNIREKAEGFLEQMNKLSNVSKIYDEME 1580
BLAST of Netrin-4 vs. Ensembl Cavefish
Match: lamb1a (laminin subunit beta-1 [Source:NCBI gene;Acc:103026996]) HSP 1 Score: 841.647 bits (2173), Expect = 0.000e+0 Identity = 548/1802 (30.41%), Postives = 895/1802 (49.67%), Query Frame = 3
Query: 63 MTLNYVFIIFV-ARYILEI-ESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQ-VKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY----------------------IEKPTYPLEHLKE--------------IKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHV--GKSSCQSY--QDTMQLI---KDSRYIVIPNK-CLEKDL--PIVLTVFTIESNNKXXXX-------------------XXXXXXXXXXXXNSTKTRKVLGECL-NYANLVSSKKCDFL-NAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTN-----SKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKL-HNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDI------------IISNFSEKLSRLKYYV---------YNSNIIDFNTQ----QNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIK----DKLFYRNEELRNINLKFIRQKSKDMDNLINEMSK-NVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISIN-----SKEIKDAYVKP-ESEYNTTIKLIDV--TRGAIQSRRIESVNLLIKIKTYLNG-SADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXX---XLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLLN---KSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKI 5090
M L ++++ A + EI E +VC + C P GDLLIGR L A+STCGL E +C+V S + KC +CDS + T SHRIENVV+ + + WWQS +G+ NV+I+++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FPS+ + +++V+C + +S I PS G V+F + D Y + +N + ITN+RV+F +LHT GD +D I+ KYYYAI + ++RG C CYGHAS+C +G + V MV+G C C HNT G +CE+C+DFY+D PW PA+ +TN C+KC CN HS SCHFD VYQ S +SGGVCD+C HNT NCE+CK +Y++ I C C+CD +G+ IC ++ LGL +G C CK NVEG +CD+CK G + + ++ GC C C +GT+ CD+ +G CYCK VTG DC C ++ LSN +++GC C C + G N C +G+C C+ G RCD+++S +Y +++P +P + ++ N+ + S Y+ +I + + + ++ ++ + V S C + D Q++ SRYIV+P C E+ L I L++ +N+ I L+ NS CL N ++V + D N S + + C C+ GS + C+ GGQCQC NV G+ C+ CA ++ F GC+ C+CD GSL++ C+ TG+C C G G+ CD C PG+W FP C C CN + CD TG+CLNCK ++ G+NC+ C NG+YGD G C+ C CP G S + + C ++ C+C +GY G CD+C ++GN G C+PC+C+ NID SC++ TG C KCL++T G C +C GY+GN + C +CVCNHMGT S + C+ +G+C CLPNVVG CD C +N++S GC C+CD P + + SCN+L+GQC C+ G GK C C++ FWG+P C C C+ G +QCN+ G C C G++G CD C +G G+ PNC C C+ +WD + + N +L + + S + P + D ++E++ ++++I P + +++ +EKL++ + ++ N+ + F+ + Q ++ +++ ++ + + ++ F + + + + S+ + + Q ++ D + EE N ++ + +DNL ++ ++ + TC + S S CGG+GC + R + C+G + N + K+F+Q ++ SK + +A VK E++ N L+ T+ + E L+ +I+ +L ADL+ E++ + + ++ ++ L +IR R+ L++V +IL++S + + + + A +A+++ + + ++ + + + +K + + + A +KG + E+E + +S T R+ L+ + + + NT + +E + LK D + K Y S+ I+E+ Q +++ K+ L + K + +L+ D E K +++K ++L+ L+ +
Sbjct: 2 MMLQLAALLYLGAISLAEIPELGDVCTEGSCYPATGDLLIGRAQQLSATSTCGLGKPEPFCIV--SHLQEEKKCFVCDSNKSFDETEHTTSHRIENVVTTFAPNRLKTWWQSENGMENVTIQLNLEAEFHFTHLIMTFKTFRPAAMVIERSADFGKTWQVYRYFAYDCESSFPSVSQGPMKKVDDVICDTRYSDIEPSTEGEVIFRVLDPVFRIEDPYSPRIQN-MLKITNLRVKFTKLHTLGDNLLDSRIEIKEKYYYAIYDMVVRGNCFCYGHASECAPVDGAGEMVDGMVHGHCMCNHNTMGLNCERCQDFYHDLPWRPAEGRNTNACKKCNCNQHSDSCHFDMAVYQASSNVSGGVCDDCLHNTMGHNCEQCKPFFYQHHERDIRDPNICEPCNCDPVGSLNGGICDALTDVSLGLISGQCRCKPNVEGERCDQCKQGHYGIS-DDPLGCKPCTCNALGTVPGGSPCDTDSGDCYCKRLVTGRDCDQCLPQHWGLSN-DMDGCRPCDC-DVGGAVNNDCSAETGQCQCREHMFGRRCDQVESGFYFIALDHYVYEAEDAKFGPGVTVVQRP-FPQDRTPTWTGIGFVNVPEGAYLEFNIDNIPHSMEYDILIRYEPQLPEQWEEVLMTVIRPRVITADSRCANTMPDDDNQMVSLHPGSRYIVLPRPVCFEQGLNYTIRLSLPRYSTNSDIQSPYTLIDSIVLMPHCKNLEIFSGSGSEGGDLETNSAWDTFQRYRCLENSQSVVKTPMPDICKNYIFSVSALLHQGAKACACDPQGSLSTVCDPNGGQCQCRANVVGRNCDTCAPATFLFGPLGCRHCECDPQGSLHAFCHEATGQCECIPGAYGRQCDRCLPGHWGFPNCRPCTCNGHAEQCDPLTGQCLNCKDHTTGHNCERCLNGHYGDPILGSGDHCRPCMCPDGPGSRRQFAGACYKNHDSYQVFCVCNQGYKGARCDECAPGYYGNPHEAGGECRPCQCNNNIDLVDPGSCDAHTGACLKCLYHTEGEGCSRCKLGYYGNALTQNCRRCVCNHMGTRKETCPSVGECHCDLSSGQCQCLPNVVGQHCDLCAPDTWNMASGRGCEACDCD-PNHSFSSSCNELSGQCSCKPGFGGKTCRECRELFWGNPEI--KCHACDCDPRGIAEQQCNKATGHCVCVEGVSGPRCDTCARGYFGEFPNCERCHQCFSEWDIIIGDLTNQTHRLMQKVNTIKSSGITGPYQ--DTINNVERSANTIRDILAQNPATQPLTEMQALLEQATTLMAEMNEKLNQTEEFMSQISDENNGTNTKLESFSEEAQKLQRTVKDLQDQVEFIRNSDVRGASDSISRYFQQSEAAEARVNASTSDPESLVQQSASVRRETEDMMNQTKEEFNNKQDEY----ATALDNLAGQLETLDLSDLSEKTCGSPAGSEACADSP-CGGLGCVDAEGIRKC-GGEGCLGLTTLAHNAWQK-----------AKDFDQDIVSAMQEVDKLSKMVLEAKVKADEAKLNAQEVLLKTNETKQRVDLSNEELRKLIKQIRDFLTQEGADLESIEAVANEVLQMQMPTTPAQLQNLTDEIRERVNSLSDVEDILNQSATDIHRAEDLLEQAQKARKEAADVKSTAEMVQEALDLAEKAQTTVTEA---LKQASTDIKGTQDLLVSVESETADSEFKLSNATVRLLQLERDVALLKEKALNTSISAEKTEEDADSINEMAEQLKKDLDTEL-KDKY----SSVEELITEKAQGVVDAKKKAEVLQQEAKDLLLQASDKLRLLKDLEKSYDENQKKLEDKANQLVDLEKAV 1766
BLAST of Netrin-4 vs. Ensembl Cavefish
Match: lamb1b (laminin subunit beta-1-like [Source:NCBI gene;Acc:103026945]) HSP 1 Score: 796.579 bits (2056), Expect = 0.000e+0 Identity = 459/1326 (34.62%), Postives = 690/1326 (52.04%), Query Frame = 3
Query: 66 TLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKC-KAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY----------------------IEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFII-------------VLSIYHVGKSSCQSY-------QDTMQLIKDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLG---------------ECLNYANLVSSKKCDFLNAE--ISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSK----WQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPK------EIDDVCTSLEQNMKSLKEI-------------------KKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKK 3758
TL + + +A+Y+ + ++ C + C P GDLLIGR L ASSTCGL E YC+V+ +G KC CDS+ Y +S +SH I+NVV+ + + WWQS +G+ NV+I++ E+EF FT + FKT+RPAAM IERS DYG++W Y YF+ +C FP I + N+++++C S +S + PS G V+F A + D Y + +N + ITN+RV+ ++LHT GD +D +R KYYYA+ + ++RG C CYGHAS+C +E + MV+G C C+H+T G +CE+C++FY D+PW PA STN C+KCECN HS CHFD VY S +SGGVCDNCQHNT+ ++C+ CK YY++ + + C C CD LG+ IC ++ GL AG C CK NVEG C+ CK G + + GCL C+C MGTL CD+ TG+CYC+ VTG C C ++ LSN +++GC C C + G N C +G+C+C+ G RCD+++S +Y + +P H I V + G F I ++M++ S +I ++ + G S ++ Q + L RY+V+P CLEK + + ++ +++ ++ V + K ++ CL V + E S + + ++C+C+ GS + C+ IGGQC C PNV GK C+ C+ ++ GC+ C+CD GSLN C TG+C C GV G+ C C +W FP C C CN S C +TG+CL C+ ++ G +C+ CE+GY+G+ S G C+ C CP G S + + C N C+C GY G C++C ++GN G C PC+C+GNI SC+S+TG C +CLF+T G C+ C GY+G+ + ++C KC+C +GT S+ + C+ +G+CPCLP VVGL CDRC +N++S+ GC PC C +P + + SC+ L+GQC C+ G G+ C C++ FWGDP C C C+ G QCN+ G C C G++G+ CD C +G G P+C C C+ +WD T ++L N + + NV+ K D +SLE +K L +I KY +G + N +E+ + + D NT +++ST+ +A + + K ++ GA + +++
Sbjct: 3 TLESLGLGLLAQYV--PDHNDKCAEGSCYPATGDLLIGRAHRLMASSTCGLNGPETYCIVSNMQAG---KCFDCDSRETYDEYSYPNSHTIDNVVTTFTPNRLKTWWQSQNGMENVTIQLDLEAEFHFTHLIMTFKTFRPAAMVIERSADYGQSWKVYRYFAFDCSESFPYISQGPVVNVDDIICDSRYSDMEPSTAGEVIFRALDPVFKIPDPYSQRIQN-LLRITNLRVRMLKLHTLGDDLLDGGEEVRRKYYYALYDMVVRGNCFCYGHASECAPVRESERGEDGMVHGHCMCKHHTTGLNCEECEEFYQDAPWKPAQGRSTNACKKCECNYHSDECHFDMAVYLSSGNVSGGVCDNCQHNTQGQHCDDCKPFYYQHPNKHLRDPRICRPCDCDPLGSLNDGICDSVTDVLKGLIAGQCRCKPNVEGESCNHCKPGHYGLS-EHPLGCLPCSCNPMGTLPGGNPCDTETGRCYCRRLVTGQYCDQCLPQFWGLSN-DMDGCRPCDC-DLGGALNNDCPPVTGQCVCREHMFGRRCDQMESGYYFASLDHYIYEAEDAAFDPAVKVVPRPLPADRHPTWTGIGFANVPEGGTLRFTIDNVPQSMEY--SLLIRYEPQLPDVWEQVMVDVKRTGFPSPSAHCSPPYADQQSASLPPGYRYVVLPRPVCLEKGQTYTVEMLMALYSSNDLHQNPHMLIDSIVLVPAVKDLELFSGSPDRQEAWTMFQKYRCLEQGQSVQKTPMTDICREYIFSASALMHQGAMECQCDPRGSVSLLCDSIGGQCSCRPNVVGKRCDTCSPGTFQLGPSGCRSCECDPMGSLNDFCDTTTGQCPCLPGVYGRQCAHCLHNHWGFPHCQECFCNGHSHHCHPQTGQCLECRDHTTGQHCERCESGYHGNALLSSGEHCRPCMCPDGPGSGRQFADTCYHNPNLRQLVCVCSPGYKGSRCEECMPGYYGNPHVVGGRCHPCQCNGNIHMHDPGSCDSRTGVCLRCLFHTEGHGCQHCRRGYYGDASTQSCQKCMCLPLGTASQSCRDGECECDRVSGQCPCLPGVVGLNCDRCAPNTWNINSKEGCQPCKC-HPTHSYSLSCDLLSGQCSCKPGFGGRACGECRELFWGDPEI--KCHACDCDPRGIATPQCNKMTGTCVCAEGVSGKRCDSCGRGYVGTFPDCQRCHKCFSEWDL-------TVNELTNQTQHLVGNVEELKVSGLVSAYQDTVSSLEGGIKELTQIVEDNKAHQTLTHSENLLRQAKYLVLGRSL---NRTEQTVGQVSADHTRALDDLNTLSEEAQRLQDSTEDKQAQVLRIKHSNIRGAADSIRE 1304
BLAST of Netrin-4 vs. Ensembl Cavefish
Match: lamb2 (laminin, beta 2 (laminin S) [Source:ZFIN;Acc:ZDB-GENE-081030-4]) HSP 1 Score: 790.03 bits (2039), Expect = 0.000e+0 Identity = 483/1409 (34.28%), Postives = 715/1409 (50.75%), Query Frame = 3
Query: 63 MTLNYVFIIFVA-RYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKI----------------------NLPPVSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQS-------YQD--TMQLIKDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXX-----XXXXXXXNSTKTRKVLGECLNYANLVSSKK------------------CDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTN-----SKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIG-YDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYA----KAMIS--KNKIRDVTGAFNELKKSLKNIL-------NGASESKKIYQNITLIKDKLFYRNEELRNIN-------LKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLM 3989
+T+ +F +A I E + + C C P GDLL+GRE L+ASSTCGLT E YC+V S + KC CDS+ PY + SHRIENV++ D + WWQS +G +V I++ E+EF FT + FKT+RPAA+ IERS D+GRTW Y YF+ +C FP I +++V+C S +S I PS G V+F D Y + +N + ITN+RV F LHT GD +D I+ KYYYA+ E ++RG C CYGHAS+C +G + ++ MV+G C C+HNTKG +CE+C DF+N+ PW PA+ +TN C+KC CN+HSS CHFD VY + +SGGVCDNC HNT +NCE CK YY++ + I + C+ C CD G+ +C + LG+ AG C CK+NVEG++CDKCK GF+ + ++ GC C C GTL CD G C+CK VTG C+ C ++ LS+ + +GC C C G+ DN+ C SG CLC++ G +C +++S ++ Y L + K+ + G F+I +M+ +D I + +++ G S D T+ + SR++V+P CLEK + L + + +K I LL +++ V G+ + A S ++ C L + +S + + P C+C+ GS ++ C+ GGQC+C PN+ G+ C++CA +YGF GC+ C+C GS C +TG+C C G GQ CD C+ G+W FP C C+CN + C +TG CL+C+ N+ G C+ C +G+YG+ G C+ C CP G +S ++ + C + C CK+GY+G C++C ++GN G CQPCRCS NID ++C+ +TG+CRKCL+NT G +C C SGY+G+G+ R C KC CN +GT S+ + +C TG+C CLPNVVGL CD C ++NL+ GC C CD P A +CN+ TGQC C+ G G+ C +C++ FWGDP L C C C+ G QCNR G C C+ G++G CD C +G G P C C C+ WD + D+ T + +EI+ G ++ EKL+ Q SI+ N+T A MI +++I + T N L+ L + N S+ ++ + + L ++L ++ + L+N N ++ KS+D + N+ + + + + T ++ L+
Sbjct: 2 LTILLLFCCALANAAIKEPDQAHGCTHGSCYPATGDLLVGREKNLKASSTCGLTRREPYCIV--SHLQEEKKCFQCDSRRPYDPQYNIISHRIENVITTFRPDRKKCWWQSENGKSDVYIQLDLEAEFHFTHLIMTFKTFRPAALLIERSADFGRTWQVYRYFAYDCASVFPGISPGPLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLK-ITNLRVNFTSLHTLGDNLLDSRLEIKEKYYYAMYELVVRGNCFCYGHASECAPIQGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFHNEHPWKPAEGRNTNACKKCNCNAHSSKCHFDMAVYLSTGNVSGGVCDNCLHNTMGRNCETCKPFYYQDPSKDIRDPRVCIACDCDPDGSVNGGMCDGHDDPSLGMIAGQCRCKQNVEGSRCDKCKPGFFGLSPDDPLGCQPCKCDPRGTLSGSSQCDPVKGDCFCKRLVTGRSCNQCLPEHWALSH-DASGCRACDCDVGGAVDNQ-CSMESGHCLCRSHMVGRQCSQVESGYFFMALDYYLYEAELAKLGQGCTVEEKEHQPGHVASWTGTGFARAPEGGTLEFIINNIPYSME-YDVLIRYELQMPRDWEEVRVTVVRPGPIPTSSPCGNTIPADDLLTVSMPSGSRFMVLPQPVCLEKGIRYTLRLEFVRYADKNSIPSFSNANILIDSIALLPRHTSLEMFVAGDPASLARKQSYERHRCHETAKSVTRPALTDACAKLISSMSAIIIDGALP--CECDPQGSVSSECDVRGGQCRCRPNIIGRRCDQCAPGTYGFGPSGCRLCECSQEGSRGRFCDENTGQCPCLPGAFGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLSCRSNTAGDKCEKCASGFYGNPILGSGNQCRPCPCPEGPNSGRHFAASCYQDNHNRQVVCNCKQGYTGVRCEECAPGYYGNPSQPGGRCQPCRCSNNIDLSDPEACDRRTGQCRKCLYNTEGSDCSICKSGYYGDGSRRNCRKCTCNFLGTERSQCLSREECLCQRPTGQCQCLPNVVGLTCDHCAPNYWNLAGGRGCESCGCD-PNNAYTSACNEFTGQCQCRDGFGGRTCRDCQENFWGDPRIL--CRACDCDRRGIETSQCNRVTGHCACRQGVSGVRCDQCARGFSGTFPACQPCHQCFGDWDRI---------------------------VQDLATKTKALAARSQEIQTTGMTGPFEKRFKELEEKLA----------------QAQSIVNARNATAEAVTNLMGMIEELRSRIGETTDTLNRLEGDLTAVQDSNYEASNELSKLEREAKELNLTSEQLNHQLDILKNSNFLGAYDSIRASYNKSRDAERRANQSTTDTPSTVSQSAGTRKKTERLI 1356
BLAST of Netrin-4 vs. Ensembl Cavefish
Match: lamb1b (laminin subunit beta-1-like [Source:NCBI gene;Acc:103026945]) HSP 1 Score: 785.793 bits (2028), Expect = 0.000e+0 Identity = 454/1308 (34.71%), Postives = 680/1308 (51.99%), Query Frame = 3
Query: 117 ESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCK-AKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY----------------------IEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFII-------------VLSIYHVGKSSCQSY-------QDTMQLIKDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLG---------------ECLNYANLVSSKKCDFLNAE--ISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSK----WQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPK------EIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISN------------FSEKLSRLKYYV------YNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKK 3758
+ ++ C + C P GDLLIGR L ASSTCGL E YC+V+ +G KC CDS+ Y +S +SH I+NVV+ + + WWQS +G+ NV+I++ E+EF FT + FKT+RPAAM IERS DYG++W Y YF+ +C FP I + N+++++C S +S + PS G V+F A + D Y + +N + ITN+RV+ ++LHT GD +D +R KYYYA+ + ++RG C CYGHAS+C +E + MV+G C C+H+T G +CE+C++FY D+PW PA STN C+KCECN HS CHFD VY S +SGGVCDNCQHNT+ ++C+ CK YY++ + + C C CD LG+ IC ++ GL AG C CK NVEG C+ CK G + + GCL C+C MGTL CD+ TG+CYC+ VTG C C ++ LSN +++GC C C + G N C +G+C+C+ G RCD+++S +Y + +P H I V + G F I ++M++ S +I ++ + G S ++ Q + L RY+V+P CLEK + + ++ +++ ++ V + K ++ CL V + E S + + ++C+C+ GS + C+ IGGQC C PNV GK C+ C+ ++ GC+ C+CD GSLN C TG+C C GV G+ C C +W FP C C CN S C +TG+CL C+ ++ G +C+ CE+GY+G+ S G C+ C CP G S + + C N C+C GY G C++C ++GN G C PC+C+GNI SC+S+TG C +CLF+T G C+ C GY+G+ + ++C KC+C +GT S+ + C+ +G+CPCLP VVGL CDRC +N++S+ GC PC C +P + + SC+ L+GQC C+ G G+ C C++ FWGDP C C C+ G QCN+ G C C G++G+ CD C +G G P+C C C+ +WD T ++L N + + NV+ K D +SLE +K L +I + + S L+R + V + + D NT +++ST+ +A + + K ++ GA + +++
Sbjct: 23 DHNDKCAEGSCYPATGDLLIGRAHRLMASSTCGLNGPETYCIVSNMQAG---KCFDCDSRETYDEYSYPNSHTIDNVVTTFTPNRLKTWWQSQNGMENVTIQLDLEAEFHFTHLIMTFKTFRPAAMVIERSADYGQSWKVYRYFAFDCSESFPYISQGPVVNVDDIICDSRYSDMEPSTAGEVIFRALDPVFKIPDPYSQRIQN-LLRITNLRVRMLKLHTLGDDLLDGGEEVRRKYYYALYDMVVRGNCFCYGHASECAPVRESERGEDGMVHGHCMCKHHTTGLNCEECEEFYQDAPWKPAQGRSTNACKKCECNYHSDECHFDMAVYLSSGNVSGGVCDNCQHNTQGQHCDDCKPFYYQHPNKHLRDPRICRPCDCDPLGSLNDGICDSVTDVLKGLIAGQCRCKPNVEGESCNHCKPGHYGLS-EHPLGCLPCSCNPMGTLPGGNPCDTETGRCYCRRLVTGQYCDQCLPQFWGLSN-DMDGCRPCDC-DLGGALNNDCPPVTGQCVCREHMFGRRCDQMESGYYFASLDHYIYEAEDAAFDPAVKVVPRPLPADRHPTWTGIGFANVPEGGTLRFTIDNVPQSMEY--SLLIRYEPQLPDVWEQVMVDVKRTGFPSPSAHCSPPYADQQSASLPPGYRYVVLPRPVCLEKGQTYTVEMLMALYSSNDLHQNPHMLIDSIVLVPAVKDLELFSGSPDRQEAWTMFQKYRCLEQGQSVQKTPMTDICREYIFSASALMHQGAMECQCDPRGSVSLLCDSIGGQCSCRPNVVGKRCDTCSPGTFQLGPSGCRSCECDPMGSLNDFCDTTTGQCPCLPGVYGRQCAHCLHNHWGFPHCQECFCNGHSHHCHPQTGQCLECRDHTTGQHCERCESGYHGNALLSSGEHCRPCMCPDGPGSGRQFADTCYHNPNLRQLVCVCSPGYKGSRCEECMPGYYGNPHVVGGRCHPCQCNGNIHMHDPGSCDSRTGVCLRCLFHTEGHGCQHCRRGYYGDASTQSCQKCMCLPLGTASQSCRDGECECDRVSGQCPCLPGVVGLNCDRCAPNTWNINSKEGCQPCKC-HPTHSYSLSCDLLSGQCSCKPGFGGRACGECRELFWGDPEI--KCHACDCDPRGIATPQCNKMTGTCVCAEGVSGKRCDSCGRGYVGTFPDCQRCHKCFSEWDL-------TVNELTNQTQHLVGNVEELKVSGLVSAYQDTVSSLEGGIKELTQIVEDNKAHQTLTHSENLLRQAKEMKLVLGRSLNRTEQTVGQVSADHTRALDDLNTLSEEAQRLQDSTEDKQAQVLRIKHSNIRGAADSIRE 1311
BLAST of Netrin-4 vs. Ensembl Cavefish
Match: lamb2 (laminin, beta 2 (laminin S) [Source:ZFIN;Acc:ZDB-GENE-081030-4]) HSP 1 Score: 634.024 bits (1634), Expect = 0.000e+0 Identity = 394/1192 (33.05%), Postives = 592/1192 (49.66%), Query Frame = 3
Query: 708 WNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKIN----------------------LPPVSKSGYYNFVITTDTKNMQFFDSFI------------IVLSIYHVGKSSCQS-------YQD--TMQLIKDSRYIVIPNK-CLEKDLPIVLTVFTIESNNKXXXXXXXXX-----XXXXXXXNSTKTRKVLGECLNYANLVSSKK------------------CDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTN-----SKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIG-YDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYA----KAMIS--KNKIRDVTGAFNELKKSLKNIL-------NGASESKKIYQNITLIKDKLFYRNEELRNIN-------LKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLM 3989
W + ND ITN+RV F LHT GD +D I+ KYYYA+ E ++RG C CYGHAS+C +G + ++ MV+G C C+HNTKG +CE+C DF+N+ PW PA+ +TN C+KC CN+HSS CHFD VY + +SGGVCDNC HNT +NCE CK YY++ + I + C+ C CD G+ +C + LG+ AG C CK+NVEG++CDKCK GF+ + ++ GC C C GTL CD G C+CK VTG C+ C ++ LS+ + +GC C C G+ DN+ C SG CLC++ G +C +++S ++ Y L + K+ + G F+I +M++ D I + +++ G S D T+ + SR++V+P CLEK + L + + +K I LL +++ V G+ + A S ++ C L + +S + + P C+C+ GS ++ C+ GGQC+C PN+ G+ C++CA +YGF GC+ C+C GS C +TG+C C G GQ CD C+ G+W FP C C+CN + C +TG CL+C+ N+ G C+ C +G+YG+ G C+ C CP G +S ++ + C + C CK+GY+G C++C ++GN G CQPCRCS NID ++C+ +TG+CRKCL+NT G +C C SGY+G+G+ R C KC CN +GT S+ + +C TG+C CLPNVVGL CD C ++NL+ GC C CD P A +CN+ TGQC C+ G G+ C +C++ FWGDP L C C C+ G QCNR G C C+ G++G CD C +G G P C C C+ WD + D+ T + +EI+ G ++ EKL+ Q SI+ N+T A MI +++I + T N L+ L + N S+ ++ + + L ++L ++ + L+N N ++ KS+D + N+ + + + + T ++ L+
Sbjct: 107 WWQSENDQLKITNLRVNFTSLHTLGDNLLDSRLEIKEKYYYAMYELVVRGNCFCYGHASECAPIQGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFHNEHPWKPAEGRNTNACKKCNCNAHSSKCHFDMAVYLSTGNVSGGVCDNCLHNTMGRNCETCKPFYYQDPSKDIRDPRVCIACDCDPDGSVNGGMCDGHDDPSLGMIAGQCRCKQNVEGSRCDKCKPGFFGLSPDDPLGCQPCKCDPRGTLSGSSQCDPVKGDCFCKRLVTGRSCNQCLPEHWALSH-DASGCRACDCDVGGAVDNQ-CSMESGHCLCRSHMVGRQCSQVESGYFFMALDYYLYEAELAKLGQGCTVEEKEHQPGHVASWTGTGFARAPEGGTLEFIINNIPYSMEY-DVLIRYELQMPRDWEEVRVTVVRPGPIPTSSPCGNTIPADDLLTVSMPSGSRFMVLPQPVCLEKGIRYTLRLEFVRYADKNSIPSFSNANILIDSIALLPRHTSLEMFVAGDPASLARKQSYERHRCHETAKSVTRPALTDACAKLISSMSAIIIDGALP--CECDPQGSVSSECDVRGGQCRCRPNIIGRRCDQCAPGTYGFGPSGCRLCECSQEGSRGRFCDENTGQCPCLPGAFGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLSCRSNTAGDKCEKCASGFYGNPILGSGNQCRPCPCPEGPNSGRHFAASCYQDNHNRQVVCNCKQGYTGVRCEECAPGYYGNPSQPGGRCQPCRCSNNIDLSDPEACDRRTGQCRKCLYNTEGSDCSICKSGYYGDGSRRNCRKCTCNFLGTERSQCLSREECLCQRPTGQCQCLPNVVGLTCDHCAPNYWNLAGGRGCESCGCD-PNNAYTSACNEFTGQCQCRDGFGGRTCRDCQENFWGDPRIL--CRACDCDRRGIETSQCNRVTGHCACRQGVSGVRCDQCARGFSGTFPACQPCHQCFGDWDRI---------------------------VQDLATKTKALAARSQEIQTTGMTGPFEKRFKELEEKLA----------------QAQSIVNARNATAEAVTNLMGMIEELRSRIGETTDTLNRLEGDLTAVQDSNYEASNELSKLEREAKELNLTSEQLNHQLDILKNSNFLGAYDSIRASYNKSRDAERRANQSTTDTPSTVSQSAGTRKKTERLI 1247 HSP 2 Score: 80.4925 bits (197), Expect = 1.446e-14 Identity = 50/127 (39.37%), Postives = 71/127 (55.91%), Query Frame = 3
Query: 63 MTLNYVFIIFVA-RYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVS-IEISFES 434
+T+ +F +A I E + + C C P GDLL+GRE L+ASSTCGLT E YC+V S + KC CDS+ PY + SHRIENV++ D + WWQS + ++ + ++F S
Sbjct: 2 LTILLLFCCALANAAIKEPDQAHGCTHGSCYPATGDLLVGREKNLKASSTCGLTRREPYCIV--SHLQEEKKCFQCDSRRPYDPQYNIISHRIENVITTFRPDRKKCWWQSENDQLKITNLRVNFTS 126
BLAST of Netrin-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008925.1 (pep scaffold:Pmarinus_7.0:GL476496:30277:70426:-1 gene:ENSPMAG00000008054.1 transcript:ENSPMAT00000008925.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 286.189 bits (731), Expect = 6.298e-79 Identity = 267/1045 (25.55%), Postives = 432/1045 (41.34%), Query Frame = 3
Query: 438 FQFTFTHLRFK-TYRPAAMYIERSKDYGRTWNKYAYFS---DNCPRDF---PSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTF-GDWKV-------DDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMS-------GGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQ-----EPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSR-------DNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPP----VSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTM-----QLIKDSRYIVIPNKCLEKDLPIVLT---VFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKC--NVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF------CHSCKC------NQMSTTCDSKTGKCLNCK---GNSFGYNCQNCENGYYGD-TSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCM-CKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSC 3377
FQ + ++ + RP +ERS D G T++ + Y+S C + P++ +EV+CTS + ++ G + S +D + K D T+ IR++ R+ T D + +P + +YYY+I + + G C C GHAS C E +++ C C+HNT G++C+ C YN W P N+ N C KC C+ + C++D V + +R +S GGVC NC NT NCE C+ G+YR V N C C CD G+ +CI+ +PE D L+ G C+C+ G +C++C + + C C C M G + D + C CK V G C C+ G+++L N GCS C C S + D + + G K + ++E + + +++ LP ++ + Y +T ++++ S+ I + S S D + Q + ++ E+ L + L F SN + + + + + + + + + + VS D + + + + D +C C G+ +C + G + + G CE C + D CD L+ C C R G HC+ C+PG++ C C C N S TC L C G C+ C + ++G+ +S G C++C+C G + G + + C+ C++ +G C++C +G+A G CQPC+C + + C+ G+C C + G CE+C G++G + C+ C C+ +GT G+ G+C C P V G CDRC + + GC PCNC + NH C+Q TG+C C G C+ C D WG GC C C+ S +C+ +G C C G GR C C +G G P C C
Sbjct: 3 FQVAYVIVKAANSPRPGNWILERSLD-GATYHPWQYYSVSDSECLTRYGITPTVGNPVYRRDDEVICTSYYVVLLMLARGKIHTSLINGRPSADDP--SPKLLDFTSARYIRLRLQRIRTLNADLMTLSHRDPREVDPIVTRRYYYSIKDISVGGMCICSGHASTCPWNEDTQKME------CQCEHNTCGENCQHCCPGYNQRRWRPGTINNGNTCEKCNCHGKTEDCYYDAEVDRTNRSLSVHGRFSGGGVCVNCSANTAGTNCETCRDGFYRPTGVLPNDPYPCRLCQCDPQGS-LSQVCIKDEKHADPEKD--LSPGQCLCRPGFAGERCERCAFAYRGY-----PDCKPCLCSMAGGTND---DPCSEPCVCKERVEGEHCDRCRAGFYDLRPRNPRGCSACFCFGLSSSCRSLPWGVTQVVDMRGWRVTDRQGLR-------KVKTFVEVDQVAVRN-ADVRRTLPALYYWLAPTSYLGNKLTAYAGHLRYSVSYDIPVDST---DSEMISDVDVIIEGNGQALSSGSLGLMLQPFEEQTLSLRLLPENFFDFRSNAPVSRDALMTALANVTRLQIRASYSSVKQAVYRLSAVS---LDVASPDAAVGSPAALDVEQCHCPHGYAGTSCESCMR--GHRRVDGTLHGGRCEPCRCHGHADD--------CD---DLSGDCMLPLSGC--RHNTMGPHCELCRPGFYGNATRGTADDCLPCTCPLSIASNNFSPTCHQDPRGVLTCDQCLPGYIGLRCERCADDFFGEPSSPGGSCRRCECNG----NEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGG-CQPCQC--HPEGATAPQCDRINGQC-PCRPSVVGRTCEQCAIGFYGLSSGAGCSPCPCHPVGT----AGVACSADGRCHCWPGVEGRSCDRCTSGHYGF-KEGGCTPCNCSH----TNHHCDQETGRCLCPPNTEGTRCHRCIDDHWGV-NPHAGCRACNCSAAHSRGARCDEASGQCSCLDGYGGRTCGECAQGRWG-YPACRPC 979 HSP 2 Score: 158.688 bits (400), Expect = 3.709e-39 Identity = 131/438 (29.91%), Postives = 190/438 (43.38%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQ-----CLPNVSGKTCERCASESYGFDARG----CKKCKCDLTGSLN---SQCYNDTGKCL----CREGVEGQHCDTCKPGYWNFPF-----CHSCKCNQ------MSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQC--PGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFG--NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQ-----------CNRNNGVCRCKPGITGRNCDMCMKGTDG 3353
C+C+ ++C+ + G C C N G CE C YG RG C C C L+ + N C+ D L C G G C+ C ++ P C C+CN CD++TG+CL C+ + G++C+ C +G+YGD + C+ CQC G ++ + + I C C+ G+ C+QC +G +S C PC C + +C S G C C G +C++C SG++G G CT C C+H TN C+ ETG+C C PN G +C RC D + ++ GC CNC + C++ +GQC C G G+ C C G WG P C C C+ +G+ C+ G C CK + G CD C+ GT G
Sbjct: 624 PCRCH---GHADDCDDLSGDCMLPLSGCRHNTMGPHCELCRPGFYGNATRGTADDCLPCTCPLSIASNNFSPTCHQDPRGVLTCDQCLPGYIGLRCERCADDFFGEPSSPGGSCRRCECNGNEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGGCQPCQCHPEGATAPQCDRIN---------GQCPCRPSVVGRTCEQCAIGFYG--LSSGAGCSPCPCHPVGTAGV--AC-SADGRC-HCWPGVEGRSCDRCTSGHYGFKEGG---CTPCNCSH--TNHH----CDQETGRCLCPPNTEGTRCHRCIDDHWGVNPHAGCRACNCSA-AHSRGARCDEASGQCSCLDGYGGRTCGECAQGRWGYPA----CRPCECHPEGTRANTCPAPPTGSLCGCDERTGQCACKENVGGTRCDACLPGTFG 1029 HSP 3 Score: 100.523 bits (249), Expect = 2.112e-21 Identity = 101/357 (28.29%), Postives = 144/357 (40.34%), Query Frame = 3
Query: 2229 QCLPNVSGKTCERCASESYGFDAR---GCKKCKC---DLTGSLNSQCYNDTGKCL-CREGVEGQHCDTCKPGYWNFPF----CHSCKCNQMSTT---CDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFG-----------------NGAV----------------RTCTKCV-------------CNHMGTNSKWQGI--------CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQ--IGCLPCNC 3089
QCLP G CERCA + +G + C++C+C + C TG+CL CRE G HC+ C G++ C C+C+ T CD G+C C+ + G C+ C G+YG +S G C C C + +G + + + C C G G+ CD+C + H+G K+G C PC CS C+ +TG C C NT G C +C+ ++G GA RTC +C C+ GT + C+ TG+C C NV G +CD C F L+ + GC C C
Sbjct: 706 QCLPGYIGLRCERCADDFFGEPSSPGGSCRRCECNGNEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGGCQPCQCHPEGATAPQCDRINGQC-PCRPSVVGRTCEQCAIGFYGLSS-GAGCSPCPC--------HPVGTAGVACSADGRCHCWPGVEGRSCDRCTSGHYG---FKEGGCTPCNCS-----HTNHHCDQETGRC-LCPPNTEGTRCHRCIDDHWGVNPHAGCRACNCSAAHSRGARCDEASGQCSCLDGYGGRTCGECAQGRWGYPACRPCECHPEGTRANTCPAPPTGSLCGCDERTGQCACKENVGGTRCDACLPGTFGLNREDPRGCTACFC 1043 HSP 4 Score: 98.2117 bits (243), Expect = 1.063e-20 Identity = 88/312 (28.21%), Postives = 126/312 (40.38%), Query Frame = 3
Query: 858 RCSC------YGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKD-FYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLE-HLKEIKINLPPVSKSG 1769
RC C +G C A+ GQ C+ T G HCE+C D FY D+ T C+ C+C H + Q +++G C+ + + CE+C G+Y ++S C C C +GT + G C C VEG CD+C G + F +E GC CNC + N CD TG+C C G C C ++ ++ GC C CS SR + CD+ SG+C C +G+ G C E + P E H + + N P +G
Sbjct: 736 RCECNGNEEAWGGGRVCDARTGQCL---------RCRERTAGFHCERCADGFYGDA-------TGTGGCQPCQC-------HPEGATAPQCDRINGQC--PCRPSVVGRTCEQCAIGFY-----GLSSGAGCSPCPCHPVGTAGVACSAD----------GRCHCWPGVEGRSCDRCTSGHYGF---KEGGCTPCNC----SHTNHHCDQETGRCLCPPNTEGTRCHRCIDDHWGVNPHA--GCRACNCSAAHSRGAR-CDEASGQCSCLDGYGGRTCGECAQGRWGYPACRPCECHPEGTRANTCPAPPTG 997 HSP 5 Score: 84.7297 bits (208), Expect = 1.590e-16 Identity = 56/170 (32.94%), Postives = 81/170 (47.65%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDAR-GCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTT--------------CDSKTGKCLNCKGNSFGYNCQNCENGYYG---DTSRGILCKKCQCPGLSSL 2621
C C+ T N++C++ G+C C PN G C RC + +G + GC+ C C S ++C +G+C C +G G+ C C G W +P C C+C+ T CD +TG+C CK N G C C G +G + RG C C C G+SS+
Sbjct: 886 PCNCSHT---NHHCDQETGRCLCPPNTEGTRCHRCIDDHWGVNPHAGCRACNCSAAHSRGARCDEASGQCSCLDGYGGRTCGECAQGRWGYPACRPCECHPEGTRANTCPAPPTGSLCGCDERTGQCA-CKENVGGTRCDACLPGTFGLNREDPRG--CTACFCFGVSSV 1049
BLAST of Netrin-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009682.1 (pep scaffold:Pmarinus_7.0:GL479835:738:11671:-1 gene:ENSPMAG00000008750.1 transcript:ENSPMAT00000009682.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 216.468 bits (550), Expect = 1.297e-62 Identity = 137/365 (37.53%), Postives = 183/365 (50.14%), Query Frame = 3
Query: 438 FQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINE------VLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDC 1514
F T+ L+F T RP + I + W Y Y+S +C P+ +R R + LCT EFS I P GG V FS + + D T IRV RL+TFGD +D+ +R+ YYYAIS++ + GRC C GHAS+C E + C C+HNT G CEKC YND PW A S NEC C+CN + C+FDP +Y+ + GG C NC+ NT+ K CERC +YR A S+ CL C+C+ +G+ + C ++ G C CK+ V G +CD+C GF +E GC C C GTLE+ C TG+C CK V G +C
Sbjct: 4 FDITYVRLKFHTSRPESFAIYKRASMDGPWTPYQYYSGSCE---PTYGKRNRGFLRSGDDEQTPLCTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPVLQDWVTSTEIRVTLNRLNTFGDEVFNDHKVLRS-YYYAISDFAVGGRCKCNGHASECVKNE-------LGALACKCRHNTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRAT--GHGGHCTNCRDNTDGKTCERCLENFYRRSA-----SEHCLPCNCNPVGS-YSRQCDEQ---------GRCSCKQGVMGERCDQCLPGFHSM---DEAGCRPCTCNPAGTLED--CQPDTGRCVCKDNVEGYNC 335 HSP 2 Score: 81.6481 bits (200), Expect = 1.772e-16 Identity = 65/208 (31.25%), Postives = 96/208 (46.15%), Query Frame = 3
Query: 2739 AISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRT-------CTKCVCNHMGTNSKWQGICNHETGK----CPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCD 3329
AIS C+C+G+ + +++ + +CR NT G +CEKC+ Y R CT C CN + TG C N G C+RC + F+ S+ CLPCNC+ P+G+ + C++ G+C C+ G G+ C+ C GF GC C CN G++ + C + G C CK + G NCD
Sbjct: 137 AISDFAVGGRCKCNGHASECVKNELGALACKCRH---NTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRATGHGGHCTNCRDNTDGKTCERCLENFYRRSASEHCLPCNCN-PVGSYSRQCDE-QGRCSCKQGVMGERCDQCLPGFHS--MDEAGCRPCTCNPAGTL-EDCQPDTGRCVCKDNVEGYNCD 336 HSP 3 Score: 73.1738 bits (178), Expect = 1.082e-13 Identity = 69/234 (29.49%), Postives = 102/234 (43.59%), Query Frame = 3
Query: 2517 NSFG---YNCQNCENGYYGDTSRGILCKKCQCPGLSS-LKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQC----ETNHFGNAISKDGT-CQPCRCSGNIDQRIEDS----CNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCN 3179
N+FG +N YY S + +C+C G +S N +G A C C+ G C++C + A S+ C C C+G D+ D G C C NT G CE+C+ ++ A C C CN +G+ S+ C+ E G+C C V+G +CD+C F ++ + GC PC C+ P G L C TG+C C+ G C+
Sbjct: 118 NTFGDEVFNDHKVLRSYYYAISDFAVGGRCKCNGHASECVKNELG--------ALACKCRHNTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRATGHGGHCTNCRDNTDGKTCERCLENFYRRSASEHCLPCNCNPVGSYSRQ---CD-EQGRCSCKQGVMGERCDQCLPGFHSM-DEAGCRPCTCN-PAGTL-EDCQPDTGRCVCKDNVEGYNCD 336 HSP 4 Score: 72.0182 bits (175), Expect = 2.754e-13 Identity = 48/113 (42.48%), Postives = 60/113 (53.10%), Query Frame = 3
Query: 2217 GGQC-QCLPNVSGKTCERCASESYGFDA-RGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNF--PFCHSCKCNQMSTT--CDSKTGKCLNCKGNSFGYNC 2537
GG C C N GKTCERC Y A C C C+ GS + QC ++ G+C C++GV G+ CD C PG+ + C C CN T C TG+C+ CK N GYNC
Sbjct: 225 GGHCTNCRDNTDGKTCERCLENFYRRSASEHCLPCNCNPVGSYSRQC-DEQGRCSCKQGVMGERCDQCLPGFHSMDEAGCRPCTCNPAGTLEDCQPDTGRCV-CKDNVEGYNC 335 HSP 5 Score: 61.2326 bits (147), Expect = 8.321e-10 Identity = 31/82 (37.80%), Postives = 48/82 (58.54%), Query Frame = 3
Query: 2163 IKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCD 2408
+ C CN GS + C++ G +C C V G+ C++C + D GC+ C C+ G+L C DTG+C+C++ VEG +CD
Sbjct: 257 LPCNCNPVGSYSRQCDEQG-RCSCKQGVMGERCDQCLPGFHSMDEAGCRPCTCNPAGTL-EDCQPDTGRCVCKDNVEGYNCD 336 HSP 6 Score: 53.9138 bits (128), Expect = 1.566e-7 Identity = 61/210 (29.05%), Postives = 82/210 (39.05%), Query Frame = 3
Query: 2310 KCKCDLTGSLNSQCYNDTGK--CLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTC---------DSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEK 2876
+CKC+ G + N+ G C CR G C+ C P Y + P+ C C CN + C G C NC+ N+ G C+ C +Y S C C C N +G C CK+G G+ CDQC G + C+PC C N +ED C TG C C N G+NC++
Sbjct: 146 RCKCN--GHASECVKNELGALACKCRHNTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRATGHGGHCTNCRDNTDGKTCERCLENFY-RRSASEHCLPCNC--------NPVGSYSRQCDEQGRCSCKQGVMGERCDQCLP---GFHSMDEAGCRPCTC--NPAGTLED-CQPDTGRC-VCKDNVEGYNCDR 337
BLAST of Netrin-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006618.1 (pep scaffold:Pmarinus_7.0:GL481588:182:19210:1 gene:ENSPMAG00000005955.1 transcript:ENSPMAT00000006618.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 205.682 bits (522), Expect = 2.374e-54 Identity = 136/495 (27.47%), Postives = 221/495 (44.65%), Query Frame = 3
Query: 2535 CQNCENGYYGDTSRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNG-AVRTCTKCVCNHMGTNSKWQGI------CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGI---TGRNCDMCMKGTDGKMPNCSSCGD-CWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKE-----------IKKYFPIGYDI--IISNFSEKLSRLKYYV-------YNSNII--DFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLIN 3917
C C +GYYG G C+ CQCP G + ++ + GCS + A C C G++G CDQC H+G+ G CQPC+C+GNID SC+ +TG C +CL +T G C+ C GY+G+ + R C KC C GT G+ C+ +G+C CLPNV GL CDRC + LS GC PC CD P + SC++ GQC+C G G C+ C++ WG+P C++C C C +P + TGR CD C +G G P C C + C+ W+ + + L + ++ +E+N+ +E +++ + D+ + + +L++L+ + ++N++ + + T + Y I + R GA+ +K S + + E+++ R+E R KF+ K +++ ++
Sbjct: 1 CCRCASGYYGSPGAGEQCRPCQCPEGPGTGRHFASGCSHDPRSRAVACACLPGFTGARCDQCSPGHYGDLRVAGGRCQPCQCNGNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYGDATSQRRCQKCTCVTAGTERSSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGLSQGTGCRPCGCD-PAHSAGPSCSEFNGQCHCLPGFGGLTCSECQENHWGEPRVK--CSECL------THNLCMAQLAYCLFRPPVRSETGRRCDRCARGFSGAFPACEPCHEPCFPDWEAAISELGRRAGGLSERATRLLTGGADAGPYARRMAEVEENLAGARELLAVRNASAAAVERLSGLIDDVGRQVEAMTGRLAQLEAGIGGAAKQNGDANVLLSELDRDAREASTRASELGYQIDTIKHSNFR---GAYGSVKDSYQQ----SREAERKASGAATNPAGPVARSEATRQRAEKFMAAKRAELEQRLD 479 HSP 2 Score: 103.219 bits (256), Expect = 3.158e-22 Identity = 92/302 (30.46%), Postives = 131/302 (43.38%), Query Frame = 3
Query: 2265 RCASESYGFDARG--CKKCKCDL---TGS-LNSQCYND----TGKCLCREGVEGQHCDTCKPGYWN-----FPFCHSCKCN-----QMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTA-----FTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNV---VGLQCDRCQDKFFNLSSQIGCLPCN 3086
RCAS YG G C+ C+C TG S C +D C C G G CD C PG++ C C+CN + +CD +TG+CL C ++ G CQNC GYYGD + C+KC C + +++ C L A C C G CD+C NH+G +S+ C+PC C + SC+ G+C CL G C +C ++G V+ C++C+ +++ C + C P V G +CDRC F C PC+
Sbjct: 3 RCASGYYGSPGAGEQCRPCQCPEGPGTGRHFASGCSHDPRSRAVACACLPGFTGARCDQCSPGHYGDLRVAGGRCQPCQCNGNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYGDATSQRRCQKCTCVTAGTERSS---CGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWG--LSQGTGCRPCGC--DPAHSAGPSCSEFNGQC-HCLPGFGGLTCSECQENHWGEPRVK-CSECLTHNL---------CMAQLAYCLFRPPVRSETGRRCDRCARGFSGAFP--ACEPCH 284 HSP 3 Score: 72.7886 bits (177), Expect = 6.182e-13 Identity = 74/259 (28.57%), Postives = 109/259 (42.08%), Query Frame = 3
Query: 2226 CQCLPNVSGKTCERCASESYGFDAR----GCKKCKC--DLTGSLNSQCYNDTGKCL-CREGVEGQHCDTCKPGYWNFPF----CHSCKCNQMST------------TCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGEC--RKCLFNTSGFNCEKCVSGYFGNGAVRTCTKC 2927
C CLP +G C++C+ YG D R C+ C+C ++ + + C TG+CL C EG C C+PGY+ C C C T CD ++G+C C N G C C ++G S+G C+ C C S + CS FN C C G+ G C +C+ NH+G K C C + C ++ C R + + +G C++C G+ +GA C C
Sbjct: 48 CACLPGFTGARCDQCSPGHYG-DLRVAGGRCQPCQCNGNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYGDATSQRRCQKCTCVTAGTERSSCGLADGACACDPRSGQC-RCLPNVQGLACDRCTPNHWG-LSQGTGCRPCGCDPAHSAGPS---CS-EFNG---QCHCLPGFGGLTCSECQENHWGEPRVK---CSEC--------LTHNLCMAQLAYCLFRPPVRSETGRRCDRCARGF--SGAFPACEPC 283 HSP 4 Score: 67.781 bits (164), Expect = 2.273e-11 Identity = 66/249 (26.51%), Postives = 97/249 (38.96%), Query Frame = 3
Query: 978 KCKDFYNDSPWLPADKNSTNECRKCEC-------NSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCI-CKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENR--------GCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE 1676
+C Y SP + +CR C+C +S C DP S V C C++C G+Y ++ VA C C C+ G P+ G C+ C + EG+ C C+ G++ + + C KC CV GT + CD +G+C C V G C C ++ LS GC C C S +C +++G+C C GF G+ C E
Sbjct: 3 RCASGYYGSP------GAGEQCRPCQCPEGPGTGRHFASGCSHDP--------RSRAVACACLPGFTGARCDQCSPGHYGDLRVA---GGRCQPCQCN--GNIDPTDAA-----SCDGRTGRCLRCLRDTEGSACQNCRPGYYG-DATSQRRCQKCTCVTAGTERSSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGLSQGT--GCRPCGCDPAHS-AGPSCSEFNGQCHCLPGFGGLTCSE 223 HSP 5 Score: 63.1586 bits (152), Expect = 5.600e-10 Identity = 45/147 (30.61%), Postives = 62/147 (42.18%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNC---------NKIGGQCQCLPNVSGKTCERCASESYGF-DARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMST--TCDSKTGKCL--NCKGNSFGYNCQNCENGYYG 2564
KC C G+ ++C + GQC+CLPNV G C+RC +G GC+ C CD S C G+C C G G C C+ +W P KC++ T C ++ CL + G C C G+ G
Sbjct: 132 KCTCVTAGTERSSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGLSQGTGCRPCGCDPAHSAGPSCSEFNGQCHCLPGFGGLTCSECQENHWGEP---RVKCSECLTHNLCMAQLAYCLFRPPVRSETGRRCDRCARGFSG 275 HSP 6 Score: 62.003 bits (149), Expect = 1.048e-9 Identity = 55/198 (27.78%), Postives = 81/198 (40.91%), Query Frame = 3
Query: 936 VCDCQHNTKGQHCEKCKDFYNDSPWLPAD-KNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCK 1526
C C G C++C SP D + + C+ C+CN + DP +G C C +TE C+ C+ GYY + S + C KC C + GT+ S + + +G C C NV+G CD+C W ++ TGC C C + C G+C+C G CS C+
Sbjct: 47 ACACLPGFTGARCDQC------SPGHYGDLRVAGGRCQPCQCNGN-----IDPTDAASCDGRTG-RCLRCLRDTEGSACQNCRPGYYGDA----TSQRRCQKCTCVTAGTERSSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGL--SQGTGCRPCGCDPAHSA-GPSCSEFNGQCHCLPGFGGLTCSECQ 225 HSP 7 Score: 56.9954 bits (136), Expect = 3.712e-8 Identity = 68/246 (27.64%), Postives = 103/246 (41.87%), Query Frame = 3
Query: 2160 PIKCKCNVTGSRNNNCNKIGGQC-QCLPNVSGKTCERCASESYGFDA---RGCKKCKCDLTGSLNSQCYNDTGKCLC--REG-------VEGQHCDTCKPGYWNFP---FCHSCKCNQMST---TCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIF--------NTTAFTC-MCKKGYSGQF--CDQCETNHFGN---AISKDGTCQPCRCSGNIDQR 2798
P +C N+ + +C+ G+C +CL + G C+ C YG DA R C+KC C G+ S C G C C R G V+G CD C P +W C C C+ + +C G+C +C G C C+ ++G+ + C +C L +N L + + T C C +G+SG F C+ C F + AIS+ G R +G + +R
Sbjct: 79 PCQCNGNIDPTDAASCDGRTGRCLRCLRDTEGSACQNCRPGYYG-DATSQRRCQKCTCVTAGTERSSCGLADGACACDPRSGQCRCLPNVQGLACDRCTPNHWGLSQGTGCRPCGCDPAHSAGPSCSEFNGQC-HCLPGFGGLTCSECQENHWGEPR--VKCSEC-------LTHNLCMAQLAYCLFRPPVRSETGRRCDRCARGFSGAFPACEPCHEPCFPDWEAAISELG-----RRAGGLSER 308
BLAST of Netrin-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008872.1 (pep scaffold:Pmarinus_7.0:GL476733:325925:343343:-1 gene:ENSPMAG00000008023.1 transcript:ENSPMAT00000008872.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 190.274 bits (482), Expect = 1.448e-50 Identity = 121/345 (35.07%), Postives = 177/345 (51.30%), Query Frame = 3
Query: 2331 GSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNF------PFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAV-RTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMC 3335
G+ + +D C C G G C+ C G+ C C C+ +T CD +G CLNC+ NS G C+ C G+YG+ G C+ C CP G S C + + C GY+G CD+C ++G+ G+CQPC+C+GN+D +C++ TGEC +C+ TSG++CE+C +GYFG+ V + C C C+ G+ S C+ TG+C C PNV G QCD C + + Q C PC C GA++ +C++ G+C C+ SGK C + +G + GC C CN G+ C+ +G C C+PG+ GR CD C
Sbjct: 335 GTRGAVAASDVELCQCPPGYSGTSCEACVRGHRRVNGSLYGGACEPCDCHGHATECDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCACPLGFSPT------CRVGPSGAMLCDRCAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFGDAIVAKNCQPCKCSSNGSQSN---TCDAVTGQCECKPNVAGRQCDHCVPNCWWDAQQRKCQPCRCSL-EGAVSSACDK-HGRCACRPEFSGKRCEPSQARHYGMSQQPTGCAPCNCNSFGAFSFDCD-ESGQCHCQPGVVGRQCDRC 667 HSP 2 Score: 122.865 bits (307), Expect = 1.226e-28 Identity = 110/337 (32.64%), Postives = 155/337 (45.99%), Query Frame = 3
Query: 2226 CQCLPNVSGKTCERCASESYGFDAR----GCKKCKCDLTGSLNSQCYNDTGKCL-CREGVEGQHCDTCKPGYW-----NFPF-CHSCKCN-QMSTTC---DSKTGKCLNCKGNSFGYNCQNCENGYYGDTS-RGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQ-IGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNC 3185
CQC P SG +CE C + C+ C C ++C + +G CL C G C+ C PG++ P C C C S TC S C C G C C +GYYG G C+ CQC G L C + T C SG C++C T +FG+AI CQPC+CS N Q ++C++ TG+C +C N +G C+ CV + + R C C C+ G S C+ + G+C C P G +C+ Q + + +S Q GC PCNC+ GA + C++ +GQC+CQ G G+ C+ C
Sbjct: 348 CQCPPGYSGTSCEACVRGHRRVNGSLYGGACEPCDCH---GHATECDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCACPLGFSPTCRVGPSGAMLCDRCAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGA-CDAV---TGECLRCVGQTSGWHCERCTTGYFGDAIVAK-NCQPCKCSSNGSQ--SNTCDAVTGQC-ECKPNVAGRQCDHCVPNCWWDAQQRKCQPCRCSLEGAVSS---ACD-KHGRCACRPEFSGKRCEPSQARHYGMSQQPTGCAPCNCNS-FGAFSFDCDE-SGQCHCQPGVVGRQCDRC 667 HSP 3 Score: 112.849 bits (281), Expect = 1.602e-25 Identity = 104/296 (35.14%), Postives = 139/296 (46.96%), Query Frame = 3
Query: 2205 CNKIGGQC-QCLPNVSGKTCERCASESYGFDARG----CKKCKCDLTGSLNSQCYNDTGKCL---CREGVEGQHCDTCKPGYWNFP-----FCHSCKCN---QMST--TCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRT-CTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRC 3035
C+ + G C C N G CERC YG G C+ C C L S + +G L C G G CD C GY+ P C C+CN +ST CD+ TG+CL C G + G++C+ C GY+GD C+ C+C SS + S C + C CK +G+ CD C N + +A + CQPCRCS ++ + +C+ K G C C SG CE + ++G T C C CN G S C+ E+G+C C P VVG QCDRC
Sbjct: 390 CDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCACPLGFSPTCR-VGPSGAMLCDRCAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFGDAIVAKNCQPCKC---SSNGSQSNTCDAVTG----QCECKPNVAGRQCDHCVPNCWWDAQQRK--CQPCRCS--LEGAVSSACD-KHGRC-ACRPEFSGKRCEPSQARHYGMSQQPTGCAPCNCNSFGAFSF---DCD-ESGQCHCQPGVVGRQCDRC 667 HSP 4 Score: 107.071 bits (266), Expect = 1.038e-23 Identity = 75/238 (31.51%), Postives = 107/238 (44.96%), Query Frame = 3
Query: 2673 CMCKKGYSGQFCDQCETNHFG-NAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGN---GAVRTCTKCVCN-------HMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIG--CLPCNCDYPIG-ALNHSCNQLTGQCY-CQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMK 3341
C C GYSG C+ C H N G C+PC C G+ + C+ +G C C N+ G CE+C+ G++GN G C C C +G + +C+ C P G +CDRC D ++ G C PC C+ + + +C+ +TG+C C SG C C G++GD C C C+ +GS C+ G C CKP + GR CD C+
Sbjct: 348 CQCPPGYSGTSCEACVRGHRRVNGSLYGGACEPCDCHGHATE-----CDDVSGNCLNCEHNSVGPRCERCLPGFYGNAQEGTPHDCQPCACPLGFSPTCRVGPSGAM--LCDR------CAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFGDAIVAKNCQPCKCSSNGSQSNTCDAVTGQCECKPNVAGRQCDHCVP 572 HSP 5 Score: 85.1149 bits (209), Expect = 7.087e-17 Identity = 85/289 (29.41%), Postives = 130/289 (44.98%), Query Frame = 3
Query: 861 CSCYGHASKCKAKEGQVQVKEMVYGVC-DCQHNTKGQHCEKC-KDFYNDSPWLPADKNSTNECRKCECN-SHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCI-CKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY--IEKPT 1709
C C+GHA++C + V G C +C+HN+ G CE+C FY + A + + ++C+ C C S +C P S M +CD C C+RC GYY + V S Q C +C+ ++ P C G C+ C G C++C G++ + C C C G+ N CD+ TG+C CK V G C C + +A C C+CS G+ + CDK+ G+C C+ F+G RC+ ++ Y ++PT
Sbjct: 381 CDCHGHATEC----------DDVSGNCLNCEHNSVGPRCERCLPGFYGN-----AQEGTPHDCQPCACPLGFSPTCRVGP-----SGAM---LCDRCAPGYAGARCDRCADGYYGSPGVPGGSCQPC-QCN-GNVDLSTPGACDAV--------TGECLRCVGQTSGWHCERCTTGYFG-DAIVAKNCQPCKCSSNGSQSNT-CDAVTGQCECKPNVAGRQCDHCVPNCW--WDAQQRKCQPCRCSLEGAVSS-ACDKH-GRCACRPEFSGKRCEPSQARHYGMSQQPT 630 HSP 6 Score: 84.3445 bits (207), Expect = 1.444e-16 Identity = 81/259 (31.27%), Postives = 109/259 (42.08%), Query Frame = 3
Query: 936 VCDCQHNTKGQHCEKCKDFY---NDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCY-CKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE-IKSEWY 1694
+C C G CE C + N S + C C+C+ H++ C D V G C NC+HN+ CERC G+Y N + C C C LG F C P + + C G +CD+C G++ G C C C L G CD+ TG+C C +G C C GYF + N C CKCS GS+ N TCD +G+C CK AG +CD + + W+
Sbjct: 347 LCQCPPGYSGTSCEACVRGHRRVNGSLY-------GGACEPCDCHGHATEC--DDV---------SGNCLNCEHNSVGPRCERCLPGFYGN--AQEGTPHDCQPCAC-PLG--FSPTCRVGPSGAMLCDR----CAPGYAGARCDRCADGYYGSPGVPGGSCQPCQCNGNVDLSTPGACDAVTGECLRCVGQTSGWHCERCTTGYFGDAIVAKN-CQPCKCSSNGSQSN-TCDAVTGQCECKPNVAGRQCDHCVPNCWW 576 HSP 7 Score: 82.0333 bits (201), Expect = 7.115e-16 Identity = 58/185 (31.35%), Postives = 85/185 (45.95%), Query Frame = 3
Query: 1050 CECNSHSSSCHFDPVVYQQSRKM-------SGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKC 1583
C C+ + C++DP V +++ + GGVC NC NT NCE C Y+R C C+C+ +G+ +CI N+ C CK G +C++C G+ F C+KC C G++ C C CKS V G C +CK G++NL +N GC+ C C
Sbjct: 1 CNCHGKAGECYYDPDVARRNLSLNTAGKFVGGGVCLNCSQNTAGVNCEICVDEYFRP------PKNPCRPCNCNPVGS-LSKVCIN--------NSRSCRCKPGYGGERCNRCAFGYKGF-----PKCVKCKCSTEGSINKDPCKE---PCICKSNVQGKHCDSCKPGHYNLQESNALGCTECFC 162 HSP 8 Score: 82.0333 bits (201), Expect = 7.490e-16 Identity = 77/251 (30.68%), Postives = 111/251 (44.22%), Query Frame = 3
Query: 936 VCD-CQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSH---SSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE 1676
+CD C G C++C D Y SP +P C+ C+CN + S+ D V G C C T +CERC +GY+ + VA N C C C S G+ Q D G C CK NV G QCD C W ++ C C C + G + + CD G+C C+ +G C + ++ +S GC+ C C+ +G+ + CD+ SG+C C+ G G +CD
Sbjct: 450 LCDRCAPGYAGARCDRCADGYYGSPGVPG-----GSCQPCQCNGNVDLSTPGACDAVT---------GECLRCVGQTSGWHCERCTTGYFGDAIVAKN----CQPCKCSSNGS-------QSNTCDA--VTGQCECKPNVAGRQCDHCVPNCWW--DAQQRKCQPCRCSLEGAVSS-ACDKH-GRCACRPEFSGKRCEPSQARHYGMSQ-QPTGCAPCNCNSFGAF-SFDCDE-SGQCHCQPGVVGRQCDR 666 HSP 9 Score: 75.0998 bits (183), Expect = 1.011e-13 Identity = 53/171 (30.99%), Postives = 85/171 (49.71%), Query Frame = 3
Query: 2061 VLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDA--RGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP----FCHSCKCN---QMSTTCDSKTGKCLNCKGNSFGYNCQNC 2546
V GECL S C+ + + + CKC+ GS++N C+ + GQC+C PNV+G+ C+ C + +DA R C+ C+C L G+++S C + G+C CR G+ C+ + ++ C C CN S CD ++G+C +C+ G C C
Sbjct: 501 VTGECLRCVGQTSGWHCERCTTGYFGDAIVAKNCQPCKCSSNGSQSNTCDAVTGQCECKPNVAGRQCDHCVPNCW-WDAQQRKCQPCRCSLEGAVSSAC-DKHGRCACRPEFSGKRCEPSQARHYGMSQQPTGCAPCNCNSFGAFSFDCD-ESGQC-HCQPGVVGRQCDRC 667 HSP 10 Score: 65.855 bits (159), Expect = 7.575e-11 Identity = 45/137 (32.85%), Postives = 67/137 (48.91%), Query Frame = 3
Query: 2217 GGQC-QCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYG-DTSRGILCKKCQCPGLSSL 2621
GG C C N +G CE C E + C+ C C+ GSL+ C N++ C C+ G G+ C+ C GY FP C CKC+ + + CK N G +C +C+ G+Y S + C +C C G++ +
Sbjct: 32 GGVCLNCSQNTAGVNCEICVDEYFRPPKNPCRPCNCNPVGSLSKVCINNSRSCRCKPGYGGERCNRCAFGYKGFPKCVKCKCSTEGSINKDPCKEPCICKSNVQGKHCDSCKPGHYNLQESNALGCTECFCFGVTRM 168 HSP 11 Score: 64.6994 bits (156), Expect = 1.675e-10 Identity = 49/145 (33.79%), Postives = 66/145 (45.52%), Query Frame = 3
Query: 2067 GECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP-----FCHSCKCNQMSTTCDS 2486
G CLN + + C+ E P C CN GS + C C+C P G+ C RCA GF C KCKC GS+N + C+C+ V+G+HCD+CKPG++N C C C ++ CDS
Sbjct: 33 GVCLNCSQNTAGVNCEICVDEYFRPPKNPCRP--CNCNPVGSLSKVCINNSRSCRCKPGYGGERCNRCAFGYKGFPK--CVKCKCSTEGSINKDPCKE--PCICKSNVQGKHCDSCKPGHYNLQESNALGCTECFCFGVTRMCDS 171 HSP 12 Score: 60.8474 bits (146), Expect = 2.435e-9 Identity = 41/116 (35.34%), Postives = 60/116 (51.72%), Query Frame = 3
Query: 2997 CLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKG 3344
C N G+ C+ C D++F C PCNC+ P+G+L+ C + C C+ G G+ CN C G+ G P C KC C+ +GS+ K + C CK + G++CD C G
Sbjct: 38 CSQNTAGVNCEICVDEYFRPPKNP-CRPCNCN-PVGSLSKVCINNSRSCRCKPGYGGERCNRCAFGYKGFPK----CVKCKCSTEGSINKDPCKE--PCICKSNVQGKHCDSCKPG 145 HSP 13 Score: 55.8398 bits (133), Expect = 7.541e-8 Identity = 57/179 (31.84%), Postives = 70/179 (39.11%), Query Frame = 3
Query: 933 GVC-DCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDS 1466
GVC +C NT G +CE C D Y P N CR C CN S V SR C+ + C RC GY C+KC C + G+ C +EP CICK NV+G CD CK G + + GC +C C + R CDS
Sbjct: 33 GVCLNCSQNTAGVNCEICVDEYFRPP--------KNPCRPCNCNPVGS---LSKVCINNSRSC------RCKPGYGGERCNRCAFGY--------KGFPKCVKCKCSTEGSINKDPC-KEP----------CICKSNVQGKHCDSCKPGHYNLQESNALGCTECFCFGV----TRMCDS 171 HSP 14 Score: 54.299 bits (129), Expect = 2.727e-7 Identity = 47/140 (33.57%), Postives = 67/140 (47.86%), Query Frame = 3
Query: 2826 GECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWG-DPTKLNGCTKCYC 3242
G C C NT+G NCE CV YF C C CN +G+ SK +C + + C C P G +C+RC F C+ C C G++N + C C++ GK C++CK G + + GCT+C+C
Sbjct: 33 GVCLNCSQNTAGVNCEICVDEYF-RPPKNPCRPCNCNPVGSLSK---VCINNSRSCRCKPGYGGERCNRCA---FGYKGFPKCVKCKCST-EGSINK--DPCKEPCICKSNVQGKHCDSCKPGHYNLQESNALGCTECFC 162
BLAST of Netrin-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004614.1 (pep scaffold:Pmarinus_7.0:GL477874:5547:15173:-1 gene:ENSPMAG00000004198.1 transcript:ENSPMAT00000004614.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 127.102 bits (318), Expect = 1.990e-33 Identity = 75/199 (37.69%), Postives = 105/199 (52.76%), Query Frame = 3
Query: 438 FQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERK--RSNINE--VLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKC-KAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPA 1019
FQ T+ LRF T RP + I + W Y ++S C R F +P R R+ E LC+ EFS I P GG V FS + + + + +TA T++RV RL+TFGD +V +P + YYYA+S+ + GRC C+GHAS+C + +G++ VC CQHNT G CE+C +N PW A
Sbjct: 1 FQITYVRLRFHTSRPESFAIYKRTSPASPWQPYQFYSGTCERTF-GLPSRGFLRAGDEERTALCSDEFSDISPLTGGNVAFSTLEGLFHSTGPLSSATQEWVTA-TDVRVSLRRLNTFGD-EVFGDPKVLRSYYYAVSDLAVGGRCKCHGHASECGRGSDGRL--------VCRCQHNTTGDDCERCLPSHNSRPWAQA 188
BLAST of Netrin-4 vs. Ensembl Nematostella
Match: EDO39502 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9X8]) HSP 1 Score: 871.692 bits (2251), Expect = 0.000e+0 Identity = 546/1619 (33.72%), Postives = 819/1619 (50.59%), Query Frame = 3
Query: 111 EIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTT-SDSGRKSKCTICDSKNPYQ---RFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKP---TYPLEHLKEIKI--------------------------------NLPPVSKSGYYNFVITTDTKNMQFFDSFIIVL----SIYHVGKSSCQSYQD------TMQLIKDSRYIVIPNK-CLEKDL-----------PIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKV-LGECLNYANLVSSKKCDFLNA----EISTNTVTKSDPIKCKCNVTGSRNNN-----CNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKK---GYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFL-IVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEI---KKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYA-----------KAMISKNKI---RD--VTGAFNELKK--------------SLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTIT----ESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDV-TRGAIQSRRIESVNLLIKIKTYLNGSADLKDFESLIEN-INKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFV 4619
+ ++ C K C P GDLL+GRE+ + ASSTCGL E YCVV+ D G C ICDS+ PYQ +FS + + + R + WWQS +G NV +++ ESEF FT +RF+T+RPA M IERS DYG+ W Y YFS +C + FP IP+ + +I++V+CT+++S++ PS G V+F A S + Y +K +N + ITNIR+ F +LHT GD +D P I+ KYYYA+ + +IRG CSCYGHA +C + GQ MVYG C+C HNT G++CE+C+D Y D PW PAD ++ NECRKC CN H+ C FDP VYQ S +SGGVC +C+HNT +NCERCK +Y++ + + C+ C+CD G C + + GL AG CICK NV G +CD+CK GF+ + GC C C +GT+ C+ TG+C CK +V G DC C Y+ LS+ GCS C C GS DN+ CDK +G+C C+ G G +C+ +K+ Y P Y E + + + ++ + SG+Y ++ +TK+ + + + + G S +D T+ L RY+ +P+ CLEK + P+ T +I +++ + + V I + + + L +CL+Y S + + S ++V + C C+ G++ + C+ IGGQC+C NV G+ C+RCA S+GF +GC C CD G++++ C TG+C CR G+ C C P W FP C C+C +++C+ KTG C++C+ N+ G NC+ C G+YG ++G CK+CQCPG SS S C + +TT + +C K GY+G+ C++C ++GN + G+C+PC C+ NID + +C+ TG C +CL+NT+GFNCE+C GYFG+ R CT CVC+ GTN + CNH+TG+C CLP+V G QCDRC + F+NLSS GC C+C G+L CNQ+TGQC C+AG G C C+D FWG P +GC+ C CN GSV QC+R+ G C CK G G CDMC T GKMP C CG+C+ QW L + +N ++ ++ + + D LE+ +K +++I +K +I + RL+ N++ I ++ T + + + A KA KN I RD + GAF +K+ SL +I KKI +TL F + ++L D+ I E+ +N + NG+ C T E CS CGG GC + + + + Q + + + M E N+S + E K +D R ++R++ L+ I +L D ++ N + ++ ++I L + IR + L V+ IL+K+K N + + K+ A A+E + + I+N++ M + + K +
Sbjct: 11 DAQASAQCAKRSCYPATGDLLVGRENKIYASSTCGLRGPEEYCVVSNLQDYG---DCHICDSR-PYQTNPQFSHVPKYMLSSFDGRKKDE---WWQSENGRENVYLQLDLESEFHFTHLIMRFRTFRPAGMVIERSWDYGKKWQIYRYFSADCDKIFPGIPKGEPKDIDDVICTNKYSTVEPSTNGEVIFRALKSQIPIKNAYSSKVQN-LLKITNIRINFTKLHTLGDDLLDPRPEIKRKYYYAVYDLVIRGSCSCYGHAEQCLPEPGQPTTPGMVYGRCNCTHNTTGKNCERCQDGYFDVPWRPADADNPNECRKCNCNGHAVKCRFDPAVYQASGNVSGGVCQDCEHNTVGRNCERCKPLHYQDPSKKFSDPDACIACNCDPTGGVNGGECEAKNDPAAGLVAGRCICKRNVGGVRCDRCKAGFFNLTTDNPDGCKACGCNPLGTIGE--CNPDTGRCTCKRYVEGEDCDRCASEYWGLSSTPA-GCSNCACDVGGSYDNQ-CDKQTGQCRCRKGITGRQCERVKTGNYYPLPDHLIYESEEARAVGVSEVARQEYPKNGAATWTGDGLLDVADGTAVVYSITNIPNSGFYTPLLRYETKDAGQWRAVVQISRPDGKPLTTGACRRPSGRDEYYRNVTIALNPSRRYVTLPDDVCLEKGVSYDIRVRFVRDPVSNTRDSIFTDSIVLLPVPGSVDIFQGSEGAKRQAQFDLYQCLDYHLAASQPRPKLSDVCKKLIFSMSSVIYDGAVACNCDPKGTQRDANGTLVCDPIGGQCKCKSNVIGQRCDRCAPGSFGFSNKGCSACDCDRIGAIDNFCNILTGQCQCRNWASGRQCSLCPPNMWGFPLCRPCRCYGHASSCNPKTGVCVDCQHNTVGKNCEACAVGFYGIATKGTPNDCKRCQCPGGSSGNKFSESCYVDTSTTPPSVLCDKCQVGYTGRQCEECADGYYGNPLVPGGSCKPCSCNNNIDPTVSGNCDRLTGSCLQCLYNTAGFNCERCKPGYFGDAIARNCTSCVCDKKGTNPGFHYSCNHKTGQCNCLPDVEGKQCDRCAEGFWNLSSGKGCQQCDCCIE-GSLRSMCNQITGQCQCKAGFGGPRCCECEDNFWGSPP--DGCSACNCNPAGSVHLQCDRDTGKCLCKVGHIGDKCDMCAADTSGKMPQCEICGECYYQWKATLGFLSRNVTYQIDRAANLTLKTTGGAYAYDKELKELEEKLKRVQKILDAQKTKENDTKMIEAELDAATKRLREIKENTDRIANEVNSTAVKTHQANDEIARLRKLLNDLLQKADQMKNDILSVRDTNIRGAFEGIKENQRRSREAEKRVNGSLDSIDRATKNRKKI--QMTLDGPPSFNQTHTKNQVDL-------GDLMKRIEELKRNSVELNGIVCGTPASKCGECTATNCS-VCGGPGCNGTRNLAQDAVKRAKEAEDAQRMREAKANMTLKETM-----EAEMMVNMSRTAAET------------AISKAMDAQMRAQNATKRMD--QLIKDILEFLAQPFPDIDMSRMLANKVLNMTLSVSPDQITKLAQDIREAWKSLTGVDEILEKTKQNLTRAEDLKRRAEEAREKALTVSDVIQNVTDALDMAANGQQLAKDAI 1585
BLAST of Netrin-4 vs. Ensembl Nematostella
Match: EDO36523 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF3]) HSP 1 Score: 256.914 bits (655), Expect = 6.218e-69 Identity = 168/506 (33.20%), Postives = 255/506 (50.40%), Query Frame = 3
Query: 123 DNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHN----VSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFP----SIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSA--DTSDWVNND-----VYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAK---EGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNA-GHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKC 1583
D+ R C P+ G+ G+ +++TCG +++CV T RK +C +C+S +P + I++ +R WWQS + + N V++ + + ++ +RF++ RP +M I + W Y YFS C + + I + + + LCT FS+I P GG V FS D D N D W +TA+ +R+ R++TFGD +V +PN+ YYYAI + I GRC C GHAS+C+ +GQ +++ C CQH+T+G CE+CK YND PW A + NEC +C CN + SC FD +Y+Q+ GG C NC+ NT+ NCERCK +YR ++ + C+ C C+ +G+ + L N+ G C CK V G +CDKC+ ++ F+ +TGC C C GT + + G+C CK V G C+ C+ GYF L +N +GC C C
Sbjct: 23 DSQGRAQRCMPQFGNAAFGKRVI--SNNTCG-DPPKKFCVQTGVLEVRK-ECEMCNSSDPALEHPALKITDIKDDTNRT------WWQSETLLENPDKPVTLTLDLGKSYDVSYIRIRFRSPRPESMAIYKKTTPSSKWIPYQYFSATCEKTYGVTTNGIVDPRNQQV--ALCTDAFSTITPLTGGNVAFSTLEDRPDAYNFDNSPALQEW------VTAVA-VRIHLRRMNTFGD-EVFGDPNVLRSYYYAIIDLSIGGRCKCNGHASECEETRLWDGQTRLQ------CKCQHDTEGVDCERCKPLYNDRPWARASSRNANECVRCNCNGLAESCVFDEALYRQT--GHGGRCQNCKQNTDGPNCERCKENFYRKTSL-----EPCVACGCNPVGS-----------VSLQCNSEGQCRCKPGVTGIKCDKCQANYFGFS---QTGCRPCQCFGPGTDPAKTQCNEKGECTCKRHVVGAKCTLCRSGYFGLEASNPHGCKQCFC 481 HSP 2 Score: 204.142 bits (518), Expect = 9.257e-53 Identity = 147/429 (34.27%), Postives = 213/429 (49.65%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWN-------FPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCM-CKKGYSGQFCDQCETNHFGNAISKDG---TCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSG---FNCEKCVSGYFGNGA---VRTCTKCVCNHMGTNSK------WQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLH---------NSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKY 3551
+C C + +G +C+ CK GY + C C+C S TCD TGKC NCK N+ G C C +GYYG+ ++G CKKC C G I C C +G+ G CD C+ ++G+ + K G TCQ C CSGNID SC+ TG+C KC+ NT CE C GY+GN C C C GT S C+ + G+C CLPNV G +CD C+ +++N++S GC PCNCD G+ N++C+ TGQC+C+ G +G+ C+ C + ++L GC C CNL GS+ C+ GVC+C+PG+ G CD C + C +C C +D +V KL+ N+S + +V F K + + +E+ + + ++Y
Sbjct: 669 QCSCPKEYKGNNCEQCKDGYTRQAGSTDRYAQCVPCQCYGHSDTCDPVTGKC-NCKHNTTGIRCDKCIDGYYGNPTQGTPDDCKKCPC---------KFGTRCIQIGANVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNCSGNIDPNAIGSCDRLTGDCLKCIHNTRNGPISQCELCAIGYYGNATHYPKPQCKACECYVQGTVSPPGHDPTLPFPCD-DKGRCTCLPNVSGDKCDTCRPEYWNVASGKGCEPCNCDT-TGSWNNTCSVTTGQCHCKPGVTGRRCDQCSIDHYAF-SEL-GCLACNCNLQGSLNVTCD-EQGVCQCRPGVLGIKCDSCQENFHNLTAGCIACPPC---YDVIQKIVGGLRRKLYDLIYIIDRFNTSSSVVVDVDFEKRLALLKEQVERLLGQSRRSQQY 1079 HSP 3 Score: 119.013 bits (297), Expect = 1.252e-26 Identity = 112/364 (30.77%), Postives = 159/364 (43.68%), Query Frame = 3
Query: 2223 QCQCLPNVSGKTCERCA---SESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF------CHSCKCNQMSTTCDSKTGK--CLNCKGNSFGYNCQNCENGYYGDT--SRG--ILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCM--CKKGYSGQFCDQCETNHFGNAIS-KDGTCQPCRC--SGNI-----DQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCY 3239
QC C G CE+C + G R + C G ++ C TGKC C+ G CD C GY+ P C C C + T C C +C G C C++GYYGD RG C+KC C G ++ N+IG C+ + G Q C+ C ++GNA C+ C C G + D + C+ K G C CL N SG C+ C Y+ + + C C C+ G+ W C+ TG+C C P V G +CD+C + S++GCL CNC+ G+LN +C++ G C C+ G G C++C++ F C CY
Sbjct: 669 QCSCPKEYKGNNCEQCKDGYTRQAGSTDRYAQCVPCQCYGHSDT-CDPVTGKCNCKHNTTGIRCDKCIDGYYGNPTQGTPDDCKKCPC-KFGTRCIQIGANVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNCSG--NIDPNAIGSCDRLTGDCLKCIHNTRNGPISQ-CELCAIGYYGNATHYPKPQCKACECYVQGTVSPPGHDPTLPFPCDDK-GRC-TCLPNVSGDKCDTCRPEYWNVASGKGCEPCNCDTTGS---WNNTCSVTTGQCHCKPGVTGRRCDQCSIDHYAF-SELGCLACNCNLQ-GSLNVTCDE-QGVCQCRPGVLGIKCDSCQENFHNLTAGCIACPPCY 1019 HSP 4 Score: 107.842 bits (268), Expect = 2.611e-23 Identity = 98/330 (29.70%), Postives = 135/330 (40.91%), Query Frame = 3
Query: 861 CSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCEC-----------------------------------------NSHSSSCH-------FDPVVYQQSRKMSGGVCDNCQHNTES---KNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLN-AGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
C CYGH+ C + V G C+C+HNT G C+KC D Y +P + + ++C+KC C ++C DP +++G C C HNT + CE C GYY N A Q C C C GT P +P L + G C C NV G++CD C+ +W + GC CNC G+ N C +TG+C+CK VTG C C I ++ S GC C C+ GS N TCD+ G C C+ G GI+CD + ++
Sbjct: 704 CQCYGHSDTC----------DPVTGKCNCKHNTTGIRCDKCIDGYYGNP----TQGTPDDCKKCPCKFGTRCIQIGANVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNCSGNIDPNAIGSCDRLTGD-CLKCIHNTRNGPISQCELCAIGYYGN-ATHYPKPQ-CKACECYVQGTVSPPG--HDPTLPFPCDDKGRCTCLPNVSGDKCDTCRPEYWNVASGK--GCEPCNCDTTGSWNNT-CSVTTGQCHCKPGVTGRRCDQCSIDHYAFSEL---GCLACNCNLQGSL-NVTCDE-QGVCQCRPGVLGIKCDSCQENFH 1006 HSP 5 Score: 107.071 bits (266), Expect = 5.218e-23 Identity = 96/334 (28.74%), Postives = 144/334 (43.11%), Query Frame = 3
Query: 2163 IKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARG----CKKCKCDLTGSLNSQCYNDTGKCLCR---EGVEGQHCDTCKPGYWNFPF--------CHSCKCN-----QMSTTCDSKTGKCLNCKGNSFG---YNCQNCENGYYGDTSR--GILCKKCQCPGLSSLKNNSIGCSLIFN-TTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQ-IGCLPC 3083
+ C+C ++ C+ + G+C C N +G C++C YG +G CKKC C ++C +C EG G CD C+ GY+ P C C C+ +CD TG CL C N+ C+ C GYYG+ + CK C+C ++ +L F C C SG CD C ++ A K C+PC C + ++C+ TG+C C +G C++C ++ + C C CN G+ + E G C C P V+G++CD CQ+ F NL++ I C PC
Sbjct: 702 VPCQCY---GHSDTCDPVTGKCNCKHNTTGIRCDKCIDGYYGNPTQGTPDDCKKCPC----KFGTRCIQIGANVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNCSGNIDPNAIGSCDRLTGDCLKCIHNTRNGPISQCELCAIGYYGNATHYPKPQCKACECYVQGTVSPPGHDPTLPFPCDDKGRCTCLPNVSGDKCDTCRPEYWNVASGKG--CEPCNC--DTTGSWNNTCSVTTGQCH-CKPGVTGRRCDQCSIDHYAFSEL-GCLACNCNLQGS----LNVTCDEQGVCQCRPGVLGIKCDSCQENFHNLTAGCIACPPC 1018 HSP 6 Score: 97.0561 bits (240), Expect = 4.781e-20 Identity = 83/283 (29.33%), Postives = 128/283 (45.23%), Query Frame = 3
Query: 2517 NSFG---YNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNH----FGNAISKDG-TCQPCRCSGNIDQRIEDSC----NSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYP-IGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWG-DPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRN 3323
N+FG + N YY + +C+C G +S + L T C C+ G C++C+ + + A S++ C C C+G + + D G C+ C NT G NCE+C ++ ++ C C CN +G+ S CN E G+C C P V G++CD+CQ +F SQ GC PC C P CN+ G+C C+ G C C+ G++G + + +GC +C+ C ++ VC+ PG T RN
Sbjct: 235 NTFGDEVFGDPNVLRSYYYAIIDLSIGGRCKCNGHASECEET---RLWDGQTRLQCKCQHDTEGVDCERCKPLYNDRPWARASSRNANECVRCNCNGLAESCVFDEALYRQTGHGGRCQNCKQNTDGPNCERCKENFYRKTSLEPCVACGCNPVGSVSLQ---CNSE-GQCRCKPGVTGIKCDKCQANYFGF-SQTGCRPCQCFGPGTDPAKTQCNE-KGECTCKRHVVGAKCTLCRSGYFGLEASNPHGCKQCF----------CYGHSTVCKAAPGYTSRN 498 HSP 7 Score: 86.2705 bits (212), Expect = 9.174e-17 Identity = 74/212 (34.91%), Postives = 98/212 (46.23%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTC---------DSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQC--PGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGA--VRTCTKCVC 2933
+C C+ EG C+ CKP Y + P+ C C CN ++ +C G+C NCK N+ G NC+ C+ +Y TS C C C G SL+ NS G C CK G +G CD+C+ N+FG S+ G C+PC+C G + CN K GEC C + G C C SGYFG A C +C C
Sbjct: 286 QCKCQHDTEGVDCERCKPLYNDRPWARASSRNANECVRCNCNGLAESCVFDEALYRQTGHGGRCQNCKQNTDGPNCERCKENFYRKTSLEP-CVACGCNPVGSVSLQCNSEG----------QCRCKPGVTGIKCDKCQANYFG--FSQTG-CRPCQCFGPGTDPAKTQCNEK-GEC-TCKRHVVGAKCTLCRSGYFGLEASNPHGCKQCFC 481 HSP 8 Score: 83.5741 bits (205), Expect = 6.887e-16 Identity = 47/114 (41.23%), Postives = 62/114 (54.39%), Query Frame = 3
Query: 2094 VSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNF 2435
VS KCD E N + C C+ TGS NN C+ GQC C P V+G+ C++C+ + Y F GC C C+L GSLN C ++ G C CR GV G CD+C+ + N
Sbjct: 897 VSGDKCDTCRPEY-WNVASGKGCEPCNCDTTGSWNNTCSVTTGQCHCKPGVTGRRCDQCSIDHYAFSELGCLACNCNLQGSLNVTC-DEQGVCQCRPGVLGIKCDSCQENFHNL 1008 HSP 9 Score: 80.8777 bits (198), Expect = 5.083e-15 Identity = 83/270 (30.74%), Postives = 111/270 (41.11%), Query Frame = 3
Query: 939 CDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFW---KFNGNEETGCLKCNCVMMGTLENRG---CDSSTGKCYCKSFVTGN----DCSTCKIGYF-NLSNANINGCSLCKCSEYGSRDNKTCDKY-------SGKCLCKNGFAGIRCDEIKSEWY 1694
C C KG +CE+CKD Y + +C C+C HS +C DPV G C NC+HNT C++C GYY N + C KC C KF + CIQ +G N C E GN CD C+ G++ T C KCNC G ++ CD TG C T N C C IGY+ N ++ C C+C G+ D G+C C +G +CD + E++
Sbjct: 670 CSCPKEYKGNNCEQCKDGYTRQ---AGSTDRYAQCVPCQCYGHSDTC--DPVT---------GKC-NCKHNTTGIRCDKCIDGYYGNPTQG--TPDDCKKCPC-----KFGTRCIQ-----IGANVVCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNC--SGNIDPNAIGSCDRLTGDCLKCIHNTRNGPISQCELCAIGYYGNATHYPKPQCKACECYVQGTVSPPGHDPTLPFPCDDKGRCTCLPNVSGDKCDTCRPEYW 910 HSP 10 Score: 75.0998 bits (183), Expect = 2.608e-13 Identity = 60/198 (30.30%), Postives = 91/198 (45.96%), Query Frame = 3
Query: 2838 KCLFNTSGFNCEKCVSGYFGNGAVRT-------CTKCVCNHMGTNSKWQGICNHETG---KCP-CLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGS--VFKQCNRNNGVCRCKPGITGRNCDMCMKGTDG-KMPNCSSCGDCW 3389
KC +T G +CE+C Y R C +C CN + + + +TG +C C N G C+RC++ F+ +S C+ C C+ P+G+++ CN GQC C+ G +G C+ C+ ++G GC C C G+ QCN G C CK + G C +C G G + N C C+
Sbjct: 288 KCQHDTEGVDCERCKPLYNDRPWARASSRNANECVRCNCNGLAESCVFDEALYRQTGHGGRCQNCKQNTDGPNCERCKENFYRKTSLEPCVACGCN-PVGSVSLQCNS-EGQCRCKPGVTGIKCDKCQANYFG--FSQTGCRPCQCFGPGTDPAKTQCNEK-GECTCKRHVVGAKCTLCRSGYFGLEASNPHGCKQCF 480 HSP 11 Score: 74.7146 bits (182), Expect = 3.685e-13 Identity = 69/234 (29.49%), Postives = 100/234 (42.74%), Query Frame = 3
Query: 2151 KSDPIKC-KCNVTGSRNNN----CNKIGGQC-QCLPNVSG---KTCERCASESYG----FDARGCKKCKCDLTGSLNSQCYN--------DTGKCLCREGVEGQHCDTCKPGYWNFPF---CHSCKCNQMST---TCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPC 2771
+ P C KCN +G+ + N C+++ G C +C+ N CE CA YG + CK C+C + G+++ ++ D G+C C V G CDTC+P YWN C C C+ + TC TG+C +CK G C C +Y + G L C G ++ + G C C+ G G CD C+ N F N + C PC
Sbjct: 797 RGAPTTCQKCNCSGNIDPNAIGSCDRLTGDCLKCIHNTRNGPISQCELCAIGYYGNATHYPKPQCKACECYVQGTVSPPGHDPTLPFPCDDKGRCTCLPNVSGDKCDTCRPEYWNVASGKGCEPCNCDTTGSWNNTCSVTTGQC-HCKPGVTGRRCDQCSIDHYAFSELGCLACNCNLQGSLNVTCDEQG----------VCQCRPGVLGIKCDSCQEN-FHNLTAGCIACPPC 1018 HSP 12 Score: 72.0182 bits (175), Expect = 2.316e-12 Identity = 59/162 (36.42%), Postives = 80/162 (49.38%), Query Frame = 3
Query: 2217 GGQCQ-CLPNVSGKTCERCASESY-GFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP--FCHSCKC-----NQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYG-DTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFT 2672
GG+CQ C N G CERC Y C C C+ GS++ QC N G+C C+ GV G CD C+ Y+ F C C+C + T C+ K G+C CK + G C C +GY+G + S CK+C C G S++ + G + TT FT
Sbjct: 346 GGRCQNCKQNTDGPNCERCKENFYRKTSLEPCVACGCNPVGSVSLQC-NSEGQCRCKPGVTGIKCDKCQANYFGFSQTGCRPCQCFGPGTDPAKTQCNEK-GEC-TCKRHVVGAKCTLCRSGYFGLEASNPHGCKQCFCYGHSTVCKAAPGYTSRNITTDFT 504 HSP 13 Score: 69.707 bits (169), Expect = 1.004e-11 Identity = 42/118 (35.59%), Postives = 60/118 (50.85%), Query Frame = 3
Query: 2163 IKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGS--LNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF-----CHSCKCNQMSTTCDSKTG 2495
+ C CN GS + CN GQC+C P V+G C++C + +GF GC+ C+C G+ +QC N+ G+C C+ V G C C+ GY+ C C C ST C + G
Sbjct: 378 VACGCNPVGSVSLQCNS-EGQCRCKPGVTGIKCDKCQANYFGFSQTGCRPCQCFGPGTDPAKTQC-NEKGECTCKRHVVGAKCTLCRSGYFGLEASNPHGCKQCFCYGHSTVCKAAPG 493 HSP 14 Score: 58.151 bits (139), Expect = 4.220e-8 Identity = 51/156 (32.69%), Postives = 73/156 (46.79%), Query Frame = 3
Query: 2922 KCVCNHMGTNSKWQGICNHETG-KCPCLPNVVGLQCDRCQ----DKFFNLSSQIG---CLPCNCDYPIGALNHSC---------NQLTGQCY-CQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMC 3335
+C CN + + + + +T +C C + G+ C+RC+ D+ + +S C+ CNC+ L SC G+C C+ G C CK+ F+ T L C C CN GSV QCN + G CRCKPG+TG CD C
Sbjct: 263 RCKCNGHASECEETRLWDGQTRLQCKCQHDTEGVDCERCKPLYNDRPWARASSRNANECVRCNCN----GLAESCVFDEALYRQTGHGGRCQNCKQNTDGPNCERCKENFY-RKTSLEPCVACGCNPVGSVSLQCN-SEGQCRCKPGVTGIKCDKC 412
BLAST of Netrin-4 vs. Ensembl Nematostella
Match: EDO36501 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF6]) HSP 1 Score: 239.58 bits (610), Expect = 1.778e-63 Identity = 166/562 (29.54%), Postives = 257/562 (45.73%), Query Frame = 3
Query: 78 VFIIFVARYILE-IESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVH----------NVSIEISFESEFQFT-FTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFP---SIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPA-DKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTL-ENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTY 1712
+F++ V++ E E++ C P + + ++STCG SE YC V + C C++ +P +R H IE + + P +WQS + V++ ISF + T F++ RP+ +E+S D G+TW+ Y+S NC +DF + P R CT ++SS P + G V F D N +++ + + T++R++ T G D ++ +YYYA+S+ + RC C+GHA C CDC HNT G CE+C +N+ W+PA + N C +CEC+SH+ SC + S++ G CD+C HNTE CERCK +YRN+++++N + C C+C G C Q G C C+ NV G QCD C FW + C +C CV GT+ + CD G+C CK V C CK GY++ C C C +YG + C K +G C+C+ G RC ++ + ++ +Y
Sbjct: 8 LFLLTVSKVNSECFENEKYDDVQTCVPEPRFAAKMKHRAVSSTSTCGSPPSE-YCRVKPLNG-----CFKCNASSPTER------HPIEYIADKSPGSDATFWQSETWWDWYQKNPRDDLKVNVSISFNKTYDITGHIMFTFESPRPSKAILEKSFDKGQTWDPLQYYSFNCRKDFGMENTDPTENRFGDFSAKCTEKYSSSSPQQNGKVTF--DFLRRYNKASFFDTQVQQYLSATDLRLRMEYPGTDGLEVAKDEVTLK-QYYYAVSDLEVIARCKCHGHARWC-------DFPHETGKNCDCLHNTTGDDCERCLPLFNNKTWMPATSETEPNPCERCECHSHADSCTY-------SQQEGYGTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNCFPQGITNNGTCNQ--------TTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVGNGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHGYWDFPKTLDTDCKRCDC-DYGGSVLQICSKTTGSCMCRTNIIGTRCTDVTTGYFTPSFSY 531 HSP 2 Score: 105.531 bits (262), Expect = 1.562e-22 Identity = 62/189 (32.80%), Postives = 94/189 (49.74%), Query Frame = 3
Query: 2799 IEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPN 3365
+ +C+ G+C +C +G C+KC G++ N C C C G++ +CN +G+CPC P V+ C RC D F+ ++ GC+ C C+ G+ + C+ +TG C C+ G C C+ GF+ + GC C C+ GS +QCN G+C CK G C+ C GT PN
Sbjct: 743 LNSTCDRYGGKC-QCKPGVTGRTCDKCKPGFY-NFTSSGCAPCDCVSTGSH---HLVCNAVSGQCPCKPGVITRTCSRCNDSFYGYTTGSGCVECRCNMS-GSADAQCDDVTGNCTCKNSTDGAKCAECRFGFYNFTSA--GCQACNCHEGGSQSQQCNLQTGICTCKSNALGEKCESCRPGTYHSDPN 923 HSP 3 Score: 100.523 bits (249), Expect = 5.081e-21 Identity = 74/232 (31.90%), Postives = 109/232 (46.98%), Query Frame = 3
Query: 2163 IKCKCNVTGS-RNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF---CHSCKCNQMSTT---CDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECR 2837
+ C C+ G+ N+ C++ GG+CQC P V+G+TC++C Y F + GC C C TGS + C +G+C C+ GV + C C ++ + C C+CN + CD TG C CK ++ G C C G+Y TS G C+ C C S S C+L C CK G+ C+ C + + + C PC C G ++C S +G R
Sbjct: 732 MACGCDSVGTYLNSTCDRYGGKCQCKPGVTGRTCDKCKPGFYNFTSSGCAPCDCVSTGSHHLVCNAVSGQCPCKPGVITRTCSRCNDSFYGYTTGSGCVECRCNMSGSADAQCDDVTGNC-TCKNSTDGAKCAECRFGFYNFTSAG--CQACNCHEGGS---QSQQCNL----QTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYCFGR-----SNTCTSASGFVR 948 HSP 4 Score: 98.5969 bits (244), Expect = 2.025e-20 Identity = 77/244 (31.56%), Postives = 108/244 (44.26%), Query Frame = 3
Query: 2661 TAFTCMCKKGYSGQFCDQC------ETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAV-----RTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQ-IG-CLPCNCDYPIGALNHS--CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLN-GCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKG 3344
T C C +G C++C +T + ++ C+ C C + D S G C C NT G CE+C ++ N ++ C C C G + G CN TG+C C NV G QCD C D F+ + + IG C C C G +N S C+Q GQC C+ + C CK G+W P L+ C +C C+ GSV + C++ G C C+ I G C G
Sbjct: 284 TGKNCDCLHNTTGDDCERCLPLFNNKTWMPATSETEPNPCERCECHSHADS-CTYSQQEGYGTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNCFPQGITNN--GTCNQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLC-VGNGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHGYWDFPKTLDTDCKRCDCDYGGSVLQICSKTTGSCMCRTNIIGTRCTDVTTG 523 HSP 5 Score: 90.8929 bits (224), Expect = 4.496e-18 Identity = 72/197 (36.55%), Postives = 102/197 (51.78%), Query Frame = 3
Query: 2820 KTGECRKCLFNTSGFNCEKCVSGYFGNGA---------VRTCTKCVCNHMG---TNSKWQGICNHETGKCP-CLPNVVGLQCDRCQDKFF-NLSSQIG----CLPCNCDYPIGALNH-SCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNG-CTKCYCNLDGSVFKQ--CNRNNGVCRCKPGITGRNCDMCMKG 3344
+TG+ CL NT+G +CE+C+ F N C +C C+ T S+ +G G C C N G C+RC+DKF+ N+S + C CNC +P G N+ +CNQ TGQC C+A +G+ C++C D FWG + G CT+C C +G+V C++ NG C CKP + R C C G
Sbjct: 283 ETGKNCDCLHNTTGDDCERCLP-LFNNKTWMPATSETEPNPCERCECHSHADSCTYSQQEGY-----GTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNC-FPQGITNNGTCNQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVGNGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHG 472 HSP 6 Score: 88.9669 bits (219), Expect = 1.526e-17 Identity = 61/191 (31.94%), Postives = 91/191 (47.64%), Query Frame = 3
Query: 2673 CMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDG-FWGDPTKLNGCTKCYC 3242
C CK G +G+ CD+C+ + S C PC C + CN+ +G+C C C +C ++G C +C CN G+ C+ TG C C + G +C C+ F+N +S GC CNC + G+ + CN TG C C++ G+ C +C+ G + DP +GCT CYC
Sbjct: 754 CQCKPGVTGRTCDKCKPGFYNFTSSG---CAPCDCVSTGSHHL--VCNAVSGQC-PCKPGVITRTCSRCNDSFYGYTTGSGCVECRCNMSGSADAQ---CDDVTGNCTCKNSTDGAKCAECRFGFYNFTS-AGCQACNC-HEGGSQSQQCNLQTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYC 933 HSP 7 Score: 87.0409 bits (214), Expect = 5.730e-17 Identity = 58/169 (34.32%), Postives = 85/169 (50.30%), Query Frame = 3
Query: 1194 RNIAVAINSS--QTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
+NI+ ++ S + C CDS+GT S C + G C CK V G CDKCK GF+ F +GC C+CV G+ + C++ +G+C CK V CS C ++ + +GC C+C+ GS D + CD +G C CKN G +C E + +Y
Sbjct: 717 KNISFSVMSEVFHGAMACGCDSVGTYLNSTCDRY--------GGKCQCKPGVTGRTCDKCKPGFYNFT---SSGCAPCDCVSTGS-HHLVCNAVSGQCPCKPGVITRTCSRCNDSFYGYTTG--SGCVECRCNMSGSADAQ-CDDVTGNCTCKNSTDGAKCAECRFGFY 870 HSP 8 Score: 85.8853 bits (211), Expect = 1.342e-16 Identity = 60/198 (30.30%), Postives = 92/198 (46.46%), Query Frame = 3
Query: 1122 GGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYP 1715
GG C C+ + C++CK G+Y +S C C C S G+ L +G C CK V C +C F+ + +GC++C C M G+ + + CD TG C CK+ G C+ C+ G++N ++A GC C C E GS+ + C+ +G C CK+ G +C+ + Y P P
Sbjct: 751 GGKC-QCKPGVTGRTCDKCKPGFYNF------TSSGCAPCDCVSTGSH---------HLVCNAVSGQCPCKPGVITRTCSRCNDSFYGYTTG--SGCVECRCNMSGSADAQ-CDDVTGNCTCKNSTDGAKCAECRFGFYNFTSA---GCQACNCHEGGSQSQQ-CNLQTGICTCKSNALGEKCESCRPGTYHSDPNNP 925 HSP 9 Score: 78.1814 bits (191), Expect = 3.262e-14 Identity = 53/181 (29.28%), Postives = 84/181 (46.41%), Query Frame = 3
Query: 2169 CKCNVTGSRNN-NCNKIGGQCQCLPNVSGKTCERCASESYGFDARG---CKKCKCDLTGSLNSQ--CYNDTGKCLCREGVEGQHCDTCKPGYWNFPF-----CHSCKCN---QMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAF 2669
C C G NN CN+ GQC+C NV+G+ C+ CA +G+ C +C C G++N+ C G+C C+ V + C CK GYW+FP C C C+ + C TG C+ C+ N G C + GY+ + + + + G +N++ + F+ T +
Sbjct: 382 CNCFPQGITNNGTCNQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVGNGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHGYWDFPKTLDTDCKRCDCDYGGSVLQICSKTTGSCM-CRTNIIGTRCTDVTTGYFTPSFSYLTFEAEEATGDYMQASNTLDLDMFFSGTGY 561 HSP 10 Score: 75.0998 bits (183), Expect = 2.569e-13 Identity = 42/122 (34.43%), Postives = 61/122 (50.00%), Query Frame = 3
Query: 2145 VTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYW-----NFPFCHSCKCNQMSTTCDSKTG 2495
T S ++C+CN++GS + C+ + G C C + G C C Y F + GC+ C C GS + QC TG C C+ G+ C++C+PG + N C C C S TC S +G
Sbjct: 824 TTGSGCVECRCNMSGSADAQCDDVTGNCTCKNSTDGAKCAECRFGFYNFTSAGCQACNCHEGGSQSQQCNLQTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYCFGRSNTCTSASG 945 HSP 11 Score: 75.0998 bits (183), Expect = 2.990e-13 Identity = 67/257 (26.07%), Postives = 107/257 (41.63%), Query Frame = 3
Query: 2355 NDTGK-CLCREGVEGQHCDTCKPGYWNFPF-----------CHSCKCNQMSTTC----DSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGY--FGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLS 3059
++TGK C C G C+ C P + N + C C+C+ + +C G C +C N+ G+ C+ C++ +Y + S + +C C C N + N T C C+ +G+ CD C +G + G C C C GN +C+ G+C C N C++C GY F C +C C++ G+ + IC+ TG C C N++G +C +F S
Sbjct: 282 HETGKNCDCLHNTTGDDCERCLPLFNNKTWMPATSETEPNPCERCECHSHADSCTYSQQEGYGTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNCFPQGITNNGTC------NQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVGNGTVNASFNCDQYNGQC-SCKPNVHTRTCQECKHGYWDFPKTLDTDCKRCDCDYGGSVLQ---ICSKTTGSCMCRTNIIGTRCTDVTTGYFTPS 528 HSP 12 Score: 71.2478 bits (173), Expect = 3.587e-12 Identity = 67/237 (28.27%), Postives = 104/237 (43.88%), Query Frame = 3
Query: 933 GVCDCQHNTKGQHCEKCKD-FYNDSPWLPADKNSTNECRKCEC---NSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSG 1631
G C C+ G+ C+KCK FYN +++ C C+C SH C+ G C C+ ++ C RC +Y + C++C C+ G+ + G+C CK + +G +C +C+ GF+ F GC CNC G+ +++ C+ TG C CKS G C +C+ G ++ N +GC+ C C +G R N TC SG
Sbjct: 752 GKCQCKPGVTGRTCDKCKPGFYN---------FTSSGCAPCDCVSTGSHHLVCN-----------AVSGQCP-CKPGVITRTCSRCNDSFY-----GYTTGSGCVECRCNMSGSA---------DAQCDDVTGNCTCKNSTDGAKCAECRFGFYNFT---SAGCQACNCHEGGS-QSQQCNLQTGICTCKSNALGEKCESCRPGTYHSDPNNPDGCTPCYC--FG-RSN-TCTSASG 945 HSP 13 Score: 66.6254 bits (161), Expect = 1.077e-10 Identity = 36/98 (36.73%), Postives = 54/98 (55.10%), Query Frame = 3
Query: 3048 FNLSSQI--GCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMC 3335
F++ S++ G + C CD LN +C++ G+C C+ G +G+ C+ CK GF+ + +GC C C GS CN +G C CKPG+ R C C
Sbjct: 721 FSVMSEVFHGAMACGCDSVGTYLNSTCDRYGGKCQCKPGVTGRTCDKCKPGFYNFTS--SGCAPCDCVSTGSHHLVCNAVSGQCPCKPGVITRTCSRC 816 HSP 14 Score: 61.2326 bits (147), Expect = 4.060e-9 Identity = 70/269 (26.02%), Postives = 108/269 (40.15%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERC---------ASESYGFDARGCKKCKCDLTGSLNSQCYNDT---GKC-LCREGVEGQHCDTCKPGYW-------NFP-FCHSCKCNQMST----TCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI-LCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYF 2894
+CKC+ + ++ G C CL N +G CERC + + C++C+C +S Y+ G C C EG C+ CK ++ N P C C C TC+ TG+C C+ N G C +C + ++G T I C +C C G N ++ S + C CK + C +C+ ++ + D C+ C C + + C+ TG C C N G C +GYF
Sbjct: 268 RCKCHGHARWCDFPHETGKNCDCLHNTTGDDCERCLPLFNNKTWMPATSETEPNPCERCECH--SHADSCTYSQQEGYGTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNCFPQGITNNGTCNQTTGQC-ECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVG-----NGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHGYWDFPKTLDTDCKRCDC--DYGGSVLQICSKTTGSC-MCRTNIIGTRCTDVTTGYF 525 HSP 15 Score: 60.8474 bits (146), Expect = 5.452e-9 Identity = 45/139 (32.37%), Postives = 65/139 (46.76%), Query Frame = 3
Query: 2058 KVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPI----KCKCNVTGSRNN--NCNKIGGQCQCLPNVSGKTCERCASESYGFDAR---GCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCH 2447
+ G+C AN V+ ++CD T+ +PI +C C G+ N NC++ GQC C PNV +TC+ C + F CK+C CD GS+ C TG C+CR + G C GY+ F +
Sbjct: 397 QTTGQCECRAN-VTGRQCDSCADTFWGYTL---EPIGYCTECLCVGNGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHGYWDFPKTLDTDCKRCDCDYGGSVLQICSKTTGSCMCRTNIIGTRCTDVTTGYFTPSFSY 531 HSP 16 Score: 57.7658 bits (138), Expect = 5.119e-8 Identity = 46/156 (29.49%), Postives = 67/156 (42.95%), Query Frame = 3
Query: 2673 CMCKKG-YSGQFCDQCETNHFGNAISKD-GTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQG-----------------ICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPC 3083
C C+ G Y+G C+ C N + C CRC+G R D C+ +TG+C+ C + G CE+C G CT+C+ + G G +CN TG+C C + G CDRC + +N S + GC+ C
Sbjct: 1122 CTCESGNYTGLSCELCNQGFTRNRPNNSYSACSLCRCTG----RSLD-CDGETGQCKGCRLGSQGLYCEQCQPGV---DNTTDCTQCLPEYWGLKEGSNGCQDCDCVLPNTLYGNSTVCNATTGQCTCKEKISGRTCDRCHENAYNTSDR-GCVEC 1268 HSP 17 Score: 56.9954 bits (136), Expect = 9.229e-8 Identity = 56/210 (26.67%), Postives = 82/210 (39.05%), Query Frame = 3
Query: 2889 YFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGK---CPCLP-NVVGLQCDRCQDKFFNL---SSQIGCLPCNCDYPIGALNHSCNQLTGQCY-CQAGRSGKMCNNCKDG-------------FWGDPTKLNGCTKCYCNLDGSVFKQ---CNRNNGVCRCKPGITGRNCDMCMKGT-DGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLH 3443
+ NG+V + + + +S QGI + G C C N GL C+ C F +S C C C + C+ TGQC C+ G G C C+ G +WG NGC C C L +++ CN G C CK I+GR CD C + + C C +C+ Q + +++ L
Sbjct: 1091 FMSNGSV------LLSEIKLHSASQGIVGEKAGHVENCTCESGNYTGLSCELCNQGFTRNRPNNSYSACSLCRCT----GRSLDCDGETGQCKGCRLGSQGLYCEQCQPGVDNTTDCTQCLPEYWGLKEGSNGCQDCDCVLPNTLYGNSTVCNATTGQCTCKEKISGRTCDRCHENAYNTSDRGCVECPECFRQLQEHVFKLRDGIKDLQ 1290 HSP 18 Score: 55.4546 bits (132), Expect = 2.350e-7 Identity = 42/141 (29.79%), Postives = 59/141 (41.84%), Query Frame = 3
Query: 2370 CLCREG-VEGQHCDTCKPGYW------NFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENG-------------YYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHF 2732
C C G G C+ C G+ ++ C C+C S CD +TG+C C+ S G C+ C+ G Y+G C+ C C L N G S + N T C CK+ SG+ CD+C N +
Sbjct: 1122 CTCESGNYTGLSCELCNQGFTRNRPNNSYSACSLCRCTGRSLDCDGETGQCKGCRLGSQGLYCEQCQPGVDNTTDCTQCLPEYWGLKEGSNGCQDCDC----VLPNTLYGNSTVCNATTGQCTCKEKISGRTCDRCHENAY 1258
BLAST of Netrin-4 vs. Ensembl Nematostella
Match: EDO40131 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S854]) HSP 1 Score: 225.713 bits (574), Expect = 3.107e-59 Identity = 208/800 (26.00%), Postives = 327/800 (40.88%), Query Frame = 3
Query: 90 FVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVT-TSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMS--GVHNVSIEISFESEFQFTFTHLRFK---TYRPAAMYIERSKDYGRTWNKYAYF------SDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLF------------SADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCK--AKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWL-----PADKNSTNECRKCECNSHSSSCHFDPVVYQQ------SRKMSGG-VCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTK---FPSI----CIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGC--DSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIP-NKCLEKDLPIV--------LTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRK--VLGECLNYAN--LVSSKKCDFLNAEISTNTVTK-SDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGC 2306
VA + L ++ + NP G + A TCGL E+Y V+ S S R + CD+ +P SH N+V + WWQS + G N++I ++ + QF ++ K YRP M +++S + G++++ + YF C F + + S N+VLCT S G V + S +W+N +T+IR++F+ + D ++YA+SE + G C+C G + CK + G Q C CQ + G +C++C YN PW P ++ C+ C C+ HS C ++ V + SR+ SGG VC +C+HNTE NCERC+ YYR + N+ C C+C G++ P + C+++ + G C CK N G +C CK GF+ N GC C+C G + + G+C CK V G C TC Y+ LS + +GC C C +YG + C+K G+C C G +CD E+Y + K + S S +F + T SF +V+S + + + + + P N+ ++L + +TV +N+ +V + ++ R+ +G C N V D A + T T + C+CN GS N C K GQC C P V+G+ C++C Y GC
Sbjct: 7 LVASFALILQQ---ISTNLANPSQGTANVAFMKQAEAQITCGLRAREKYFAVSQMSQSPRNRVESTCDAYDP------ELSHNASNLVD---NNESTWWQSTASVGTANITIRLTGPAFKQFYVDTIQIKFGEYYRPRQMALKKSINNGQSFDDWHYFVSYPDPDKQCKEKFDETLQTQPSKENDVLCTGYPDVNSQSIGQVVEWKPHFLRGQLENPSQKLLEWIN--------------VTDIRLEFITMERVFDVLA------AKWHHYAVSEVRVFGFCACNGQGTGCKFNSTVGDFQ--------CVCQPGSCGYYCDECCPAYNQYPWKKGGKGPLIVDTAAACQPCNCHDHSFVCVYNETVARMNASLDMSRQYSGGGVCQDCKHNTEGINCERCRPLYYRPENRSQNAVDACESCNCSEAGSRDHALPGVLHLDCLRDVNASETMKPGDCFCKNNTMGRRCHLCKPGFFNLTSNNPLGCQVCDCDTAGARNSSNICEGDAHGQCPCKQNVQGLRCDTCNNTYYGLSRQDTHGCRPCLC-DYGGSSSPVCNKTDGQCKCLPNIDGRQCDRPLDEYYFPDLHFIGADFSHQKSEVGQSSVSWVTSFTVVDRTNKTS--GSFSLVISYQSATRQEVTGSVTSPEGTTTQQVTLPPCNQTCHQNLSVFGEMSLTGKVTVNVTAANDDFRGVRASVVPREFYEASALGDRRDAFIGNCSVTTNSVRVGEDAEDLCKAGVFTLTTDYLGQALACECNRDGSVNLTCQKYAGQCHCKPGVTGRKCDQCQQGFYNLTTTGC 763 HSP 2 Score: 144.05 bits (362), Expect = 2.706e-34 Identity = 100/317 (31.55%), Postives = 155/317 (48.90%), Query Frame = 3
Query: 2163 IKCKCNVTGSRNNNCNKIGGQC-QCLPNVSGKTCERCASESYGFDARG----CKKCKCDLTGSLNSQCYNDTGKCLCR--EGVEGQHCDTCKPGYWNF-----PF---CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCPG---LSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFN 3053
I C CN + N+C G C C N +G+ CE C YG A G CK+C CD + ++D + + G+ C PG+ PF C C CN + C + TG C NCK N+ G +C+ C++G+YG+T+ G CK+C C +S+ G + N C +GY G C CE+ +FG + G C C+C+ N + CN+ TG+C C +NT+GF+CE+C + +G+ + C C+C+ G+ + +C+++TG+C C PNV C +C+ + N
Sbjct: 1553 IPCNCN---NHTNDCYADTGLCYNCKHNTAGEHCELCDDGFYGNAADGTPGDCKQCPCD--APRTTTAFSDVRRLQAKSDRGIRSGTEHRCAPGHHRVNVSGDPFYGKCIPCNCNNHTNDCYADTGLCYNCKHNTTGEHCELCDDGFYGNTTDGTPGDCKQCPCDAPRTTTSMCMEDAGRPVCLN-------CSEGYEGVTCQTCESRYFGKPLDPGGNCSLCQCNNNT-----EICNTTTGQCTNCQYNTTGFHCERCQNETYGDAKKQQCVDCLCDSTGS---YNNVCDYKTGQCECKPNVGLRNCSKCEVSWLN 1849 HSP 3 Score: 125.176 bits (313), Expect = 1.724e-28 Identity = 106/349 (30.37%), Postives = 149/349 (42.69%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWNF-----PF---CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFT------CMCKKGYSGQFCDQCETNHFGNAISKD---GTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGN---GAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCL---PNVVGLQCDRCQDKFFNLSSQIG--CLPCNCDYPIGALNHSCNQLTGQCY-CQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMC 3335
+C C G C+ C PG+ PF C C CN + C + TG C NCK N+ G +C+ C++G+YG+ + G QCP C TTAF+ +G +C H +S D G C PC C+ + + C + TG C C NT+G +CE C G++GN G C +C C+ T + +C + G+ CL G+ C C+ ++F G C C C+ CN TGQC CQ +G C C++ +GD K C C C+ GS C+ G C CKP + RNC C
Sbjct: 1519 QCACGPEYSGLSCERCAPGHHRVNVSGDPFYGKCIPCNCNNHTNDCYADTGLCYNCKHNTAGEHCELCDDGFYGNAADGTPGDCKQCP-----------CDAPRTTTAFSDVRRLQAKSDRGIRSGTEHRCAPGHHRVNVSGDPFYGKCIPCNCNNHTND-----CYADTGLCYNCKHNTTGEHCELCDDGFYGNTTDGTPGDCKQCPCDAPRTTT---SMCMEDAGRPVCLNCSEGYEGVTCQTCESRYFGKPLDPGGNCSLCQCNNNT----EICNTTTGQCTNCQYNTTGFHCERCQNETYGDAKKQQ-CVDCLCDSTGSYNNVCDYKTGQCECKPNVGLRNCSKC 1843 HSP 4 Score: 100.138 bits (248), Expect = 6.061e-21 Identity = 87/283 (30.74%), Postives = 122/283 (43.11%), Query Frame = 3
Query: 2628 NSIGCSLIFNTTA--FTCMCKKGYSGQFCDQCETNHFGNAISKDG----------TCQPCRCSGN--------IDQRIEDSCN-----SKTGECRKCLFNTSGFNCEKCVSGYF-----GNGAVRTCTKCVCNHMGT-NSKWQGICN------------HETGKCPCLPNVVGLQCDRCQDKFFNLSSQ--IGCLPCNCDYPIGALNHSC---NQLTGQCYCQAGRSGKMCNNCKDGFWGDPTK-LNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCD 3329
N G FN+T F C+C+ G G +CD+C + K G CQPC C + R+ S + S G C+ C NT G NCE+C Y+ AV C C C+ G+ + G+ + + G C C N +G +C C+ FFNL+S +GC C+CD GA N S GQC C+ G C+ C + ++G + +GC C C+ GS CN+ +G C+C P I GR CD
Sbjct: 259 NGQGTGCKFNSTVGDFQCVCQPGSCGYYCDECCPAYNQYPWKKGGKGPLIVDTAAACQPCNCHDHSFVCVYNETVARMNASLDMSRQYSGGGVCQDCKHNTEGINCERCRPLYYRPENRSQNAVDACESCNCSEAGSRDHALPGVLHLDCLRDVNASETMKPGDCFCKNNTMGRRCHLCKPGFFNLTSNNPLGCQVCDCD-TAGARNSSNICEGDAHGQCPCKQNVQGLRCDTCNNTYYGLSRQDTHGCRPCLCDYGGSSSPVCNKTDGQCKCLPNIDGRQCD 540 HSP 5 Score: 80.8777 bits (198), Expect = 5.284e-15 Identity = 76/277 (27.44%), Postives = 110/277 (39.71%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWNFPF---------------CHSCKCNQMSTTCD------------------SKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKNNSIG-----CSLIFNTTAFT----CMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKT-GECRKCLFNTSGFNCEKCVSGYFGNGAVRT--CTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFF 3050
+C+C+ G G +CD C P Y +P+ C C C+ S C S G C +CK N+ G NC+ C YY +R C+ C C S + G C N + C CK G+ C C+ F + CQ C C + + C G+C C N G C+ C + Y+G T C C+C++ G++S +CN G+C CLPN+ G QCDR D+++
Sbjct: 275 QCVCQPGSCGYYCDECCPAYNQYPWKKGGKGPLIVDTAAACQPCNCHDHSFVCVYNETVARMNASLDMSRQYSGGGVCQDCKHNTEGINCERCRPLYYRPENRSQNAVDACESCNCSEAGSRDHALPGVLHLDCLRDVNASETMKPGDCFCKNNTMGRRCHLCKPGFFNLTSNNPLGCQVCDCDTAGARNSSNICEGDAHGQC-PCKQNVQGLRCDTCNNTYYGLSRQDTHGCRPCLCDYGGSSSP---VCNKTDGQCKCLPNIDGRQCDRPLDEYY 547 HSP 6 Score: 75.485 bits (184), Expect = 2.080e-13 Identity = 84/323 (26.01%), Postives = 130/323 (40.25%), Query Frame = 3
Query: 861 CSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKD-FY-NDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSG-------------------------------------------GVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCY-CKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEW 1691
C+C H + C A G + Y +C+HNT G+HCE C D FY N + P D C++C C++ ++ F V Q++ G G+C NC+HNT ++CE C G+Y N + C +C CD+ T S+C+++ + LN C E EG C C+ ++ + C C C + C+++TG+C C+ TG C C+ + +A C C C GS +N CD +G+C CK C + + W
Sbjct: 1555 CNCNNHTNDCYADTG------LCY---NCKHNTAGEHCELCDDGFYGNAADGTPGD------CKQCPCDAPRTTTAFSDVRRLQAKSDRGIRSGTEHRCAPGHHRVNVSGDPFYGKCIPCNCNNHTNDCYADTGLCYNCKHNTTGEHCELCDDGFYGNTTDG--TPGDCKQCPCDAPRTT-TSMCMEDAGRPVCLN-----CSEGYEGVTCQTCESRYFGKPLDPGGNCSLCQCNNNTEI----CNTTTGQCTNCQYNTTGFHCERCQNETY--GDAKKQQCVDCLCDSTGSYNN-VCDYKTGQCECKPNVGLRNCSKCEVSW 1847 HSP 7 Score: 70.0922 bits (170), Expect = 8.394e-12 Identity = 93/393 (23.66%), Postives = 156/393 (39.69%), Query Frame = 3
Query: 630 SSIIPSEGGTVLFSADTSDWVNNDVYWN-----KKRNDIT----------AITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISE-WIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYN-----DSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDS--LGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFN--GNEETG-CLKCNCVMMGTLENRGCDSSTGKCY-CKSFVTGNDCSTCKIGYF-NLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKN---GFAGIRCDEIKSEWYIEKPTYP 1715
+S++ + GG + F+ S+ N + N K +++T A+ R F+R ++ ++ P R + +++ I S Y + V + + C C G CE+C ++ P+ +C C CN+H++ C+ D G+C NC+HNT ++CE C G+Y N A + C +C CD+ T F + + + D G+ +G +C G + N G+ G C+ CNC C + TG CY CK TG C C G++ N ++ C C C + C + +G+ +C N G+ G+ C +S Y KP P
Sbjct: 1402 TSLVGAYGGNLSFTLHMSNQSGNTIGPNVKVIMKGLHNVTIEAAVASIPVAVPTTRSVFMREISWL-YRGSSEPVSRAHFMLVLAKPRAILVTASLYTSGGQDYFSSPPVGDRALEVEQCACGPEYSGLSCERCAPGHHRVNVSGDPFY-------GKCIPCNCNNHTNDCYADT-----------GLCYNCKHNTAGEHCELCDDGFYGN--AADGTPGDCKQCPCDAPRTTTAFSDVRRLQAKSDRGIRSG-----------TEHRCAPGHHRVNVSGDPFYGKCIPCNC----NNHTNDCYADTGLCYNCKHNTTGEHCELCDDGFYGNTTDGTPGDCKQCPC-DAPRTTTSMCMEDAGRPVCLNCSEGYEGVTCQTCESR-YFGKPLDP 1756 HSP 8 Score: 59.3066 bits (142), Expect = 1.642e-8 Identity = 39/110 (35.45%), Postives = 55/110 (50.00%), Query Frame = 3
Query: 2145 VTKSDPIKCK---CNVTGSRN--NNCN-KIGGQCQCLPNVSGKTCERCASESYGF---DARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCH 2447
+T ++P+ C+ C+ G+RN N C GQC C NV G C+ C + YG D GC+ C CD GS + C G+C C ++G+ CD Y+ FP H
Sbjct: 444 LTSNNPLGCQVCDCDTAGARNSSNICEGDAHGQCPCKQNVQGLRCDTCNNTYYGLSRQDTHGCRPCLCDYGGSSSPVCNKTDGQCKCLPNIDGRQCDRPLDEYY-FPDLH 552 HSP 9 Score: 58.9214 bits (141), Expect = 2.205e-8 Identity = 77/300 (25.67%), Postives = 116/300 (38.67%), Query Frame = 3
Query: 2169 CKCNVTGSRNNNCNKIGG-QCQCLPNVSGKTCERC--ASESYGFD-----------ARGCKKCKCD--------------LTGSLN-SQCYNDTGKCL-CREGVEGQHCDTCKPGYWN--------FPFCHSCKCNQ-------------------MSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL-CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYF 2894
C CN G+ + +G QC C P G C+ C A Y + A C+ C C + SL+ S+ Y+ G C C+ EG +C+ C+P Y+ C SC C++ ++ + K G C CK N+ G C C+ G++ TS L C+ C C + +N+S C C CK+ G CD C ++G + C+PC C + CN G+C KCL N G C++ + Y+
Sbjct: 256 CACNGQGTGCKFNSTVGDFQCVCQPGSCGYYCDECCPAYNQYPWKKGGKGPLIVDTAAACQPCNCHDHSFVCVYNETVARMNASLDMSRQYSGGGVCQDCKHNTEGINCERCRPLYYRPENRSQNAVDACESCNCSEAGSRDHALPGVLHLDCLRDVNASETMKPGDCF-CKNNTMGRRCHLCKPGFFNLTSNNPLGCQVCDC-DTAGARNSSNICE---GDAHGQCPCKQNVQGLRCDTCNNTYYGLSRQDTHGCRPCLC--DYGGSSSPVCNKTDGQC-KCLPNIDGRQCDRPLDEYY 547
BLAST of Netrin-4 vs. Ensembl Nematostella
Match: EDO36500 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF5]) HSP 1 Score: 218.394 bits (555), Expect = 4.973e-57 Identity = 169/590 (28.64%), Postives = 267/590 (45.25%), Query Frame = 3
Query: 135 RKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPF-----RWWQS----------MSGVHN---VSIEISFESEFQFTFTHLR--FKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNIN--EVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTL-ENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYG-------SRD---NKTCDKY-------------SGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKS 1766
R T C P + + R + S+TCG T + +YC + + C CD+ + +S H + N+V PF WWQS +S + N V+I +SF +Q + H++ F + RP AM ++S D+G TW ++ C R + P+ N ++ CT E+S P + G V F A + + Y+N + D ++T++R++ + G + + NK YYAIS + GRCSC GHA C G + + C+C+HNT G CE C+ +N+ PW+ A++ NEC++C CN+H++SC VY ++ + G+C CQHNT+ +C+ C + +YR+++ IN CL T S Q + + G C CK NV G +CD+C G W F + C CNC + GT CD TG+C+CK + G C C+ G+ GC+ C+C G S D + C +Y +G C C+ G G C + + Y L + I+ ++ +
Sbjct: 39 RATPCMPFTSFMSLTRN--VTTSNTCGTTRA-KYCELPGPE-----PCYYCDANSS----NSNEQHPVTNLVDNELVLPFYAQKYTWWQSQTWWETNQLGLSSIENPPKVNITLSFGKRYQLS-GHIKATFYSKRPKAMCFDKSNDFGHTWETLLCYAWRCDRFYNMEPQSNPDPANPFKLYCTEEYSGHEPRKLGIVEFIASKRYEICD--YFNPEVQDYLSVTDVRLRLEYPASDGMELLYQDEQTLNKLYYAISNVEVTGRCSCNGHAMGCS---GPIMSRR-----CECEHNTMGTDCEMCEPLFNNRPWMRANQTHGNECQECNCNNHATSC-----VYNET--LGYGLCKGCQHNTQGDHCQNCTAKHYRDLSKPINHVNACLGI------TNNGSCLTQATDTE---QIGQCSCKPNVYGRRCDQCIPGHWGFTLPPQGECKACNCNISGTRNATINCDQLTGQCFCKPSIQGVYCDGCRDGFHTFPTQGDGGCAPCQCDLGGGFSICDKSSDGICTQKCVEYTLPTYIHISIASNAGSCTCRKGVEGFSCKQALYGTFYPALDYLLHEAETTGISATTITPA 589 HSP 2 Score: 84.7297 bits (208), Expect = 3.175e-16 Identity = 86/316 (27.22%), Postives = 129/316 (40.82%), Query Frame = 3
Query: 2514 GNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAI------SKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSK--WQGICNHETGKCPCLPNVVGLQCDRCQDKF--FNLSSQIGCLPCNCDYPIGALNHS--CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLN-GCTKCYCNLDGSVFKQCNR-------------------------NNGVCRCKPGITGRNCDMCMKGT 3347
G Y + N Y S + +C C G +++GCS + C C+ G C+ CE F N + CQ C C+ + + + G C+ C NT G +C+ C + ++ + + C + N Q + G+C C PNV G +CD+C F L Q C CNC+ G N + C+QLTGQC+C+ G C+ C+DGF PT+ + GC C C+L G F C++ N G C C+ G+ G +C + GT
Sbjct: 261 GMELLYQDEQTLNKLYYAISNVEVTGRCSCNG------HAMGCSGPI--MSRRCECEHNTMGTDCEMCEP-LFNNRPWMRANQTHGNECQECNCNNHATSCVYNE-TLGYGLCKGCQHNTQGDHCQNCTAKHYRDLSKPINHVNACLGITNNGSCLTQATDTEQIGQCSCKPNVYGRRCDQCIPGHWGFTLPPQGECKACNCNIS-GTRNATINCDQLTGQCFCKPSIQGVYCDGCRDGFHTFPTQGDGGCAPCQCDLGGG-FSICDKSSDGICTQKCVEYTLPTYIHISIASNAGSCTCRKGVEGFSCKQALYGT 564 HSP 3 Score: 79.337 bits (194), Expect = 1.316e-14 Identity = 49/158 (31.01%), Postives = 68/158 (43.04%), Query Frame = 3
Query: 2169 CKCNVTGSRN-NNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIG 2639
C+C+ GS N C GGQC C P V+G C C Y + GCKKC+C ++GS+ C N GKC C+ VEG CD CK G ++ C +C G +NC + + + + Q P L L + +G
Sbjct: 763 CECDPEGSTNPTQCVAYGGQCPCKPGVAGLKCNHCLPGYYALSSSGCKKCECSMSGSITPVCDNADGKCQCKANVEGAKCDACKYGTFHLD--------------SGNPHGCQHCFGYGHAHNCTSASGFIATNITSNFITASMQRPFLKILFDFPLG 906 HSP 4 Score: 72.0182 bits (175), Expect = 2.283e-12 Identity = 47/131 (35.88%), Postives = 64/131 (48.85%), Query Frame = 3
Query: 1239 CHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSG 1631
C CD G+ P+ C+ G C CK V G +C+ C G++ + +GC KC C M G++ CD++ GKC CK+ V G C CK G F+L + N +GC C YG N C SG
Sbjct: 763 CECDPEGSTNPTQCVAY--------GGQCPCKPGVAGLKCNHCLPGYYALS---SSGCKKCECSMSGSI-TPVCDNADGKCQCKANVEGAKCDACKYGTFHLDSGNPHGCQ--HCFGYGHAHN--CTSASG 877 HSP 5 Score: 68.5514 bits (166), Expect = 2.649e-11 Identity = 60/210 (28.57%), Postives = 90/210 (42.86%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTCDSKT----GKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAF-TCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGY--FGNGAVRTCTKCVCNHMG 2945
+C C G C+ C+P + N P+ C C CN +T+C G C C+ N+ G +CQNC +Y D S+ I C G++ NN + +T C CK G+ CDQC H+G + G C+ C C+ + + +C+ TG+C C + G C+ C G+ F C C C+ G
Sbjct: 305 RCECEHNTMGTDCEMCEPLFNNRPWMRANQTHGNECQECNCNNHATSCVYNETLGYGLCKGCQHNTQGDHCQNCTAKHYRDLSKPINHVNA-CLGIT---NNGSCLTQATDTEQIGQCSCKPNVYGRRCDQCIPGHWGFTLPPQGECKACNCNISGTRNATINCDQLTGQC-FCKPSIQGVYCDGCRDGFHTFPTQGDGGCAPCQCDLGG 509 HSP 6 Score: 66.6254 bits (161), Expect = 1.091e-10 Identity = 39/109 (35.78%), Postives = 61/109 (55.96%), Query Frame = 3
Query: 3030 RCQDKFFNLSSQI--GCLPCNCDYPIGALNHS-CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGT 3347
+C+ F+ S+++ G C CD P G+ N + C GQC C+ G +G CN+C G++ + +GC KC C++ GS+ C+ +G C+CK + G CD C GT
Sbjct: 744 QCRALVFSASTELYNGTKVCECD-PEGSTNPTQCVAYGGQCPCKPGVAGLKCNHCLPGYYALSS--SGCKKCECSMSGSITPVCDNADGKCQCKANVEGAKCDACKYGT 849 HSP 7 Score: 60.077 bits (144), Expect = 9.872e-9 Identity = 71/274 (25.91%), Postives = 107/274 (39.05%), Query Frame = 3
Query: 2223 QCQCLPNVSGKTCERC-----------ASESYGFDARGCKKCKCDLTGSLNSQCYNDT---GKCL-CREGVEGQHCDTCKPGYW---NFPFCHSCKCNQMS--------TTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRC----------------------SGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYF 2894
+C+C N G CE C A++++G + C++C C+ S YN+T G C C+ +G HC C ++ + P H C ++ T + G+C +CK N +G C C G++G T +G CK C C +S +N +I C + C CK G +CD C DG C PC+C + I S S G C C GF+C++ + G F
Sbjct: 305 RCECEHNTMGTDCEMCEPLFNNRPWMRANQTHGNE---CQECNCN--NHATSCVYNETLGYGLCKGCQHNTQGDHCQNCTAKHYRDLSKPINHVNACLGITNNGSCLTQATDTEQIGQC-SCKPNVYGRRCDQCIPGHWGFTLPPQG-ECKACNC-NISGTRNATINCDQLTG----QCFCKPSIQGVYCDGCRDGFHTFPTQGDGGCAPCQCDLGGGFSICDKSSDGICTQKCVEYTLPTYIHISIASNAGSC-TCRKGVEGFSCKQALYGTF 565 HSP 8 Score: 58.9214 bits (141), Expect = 2.179e-8 Identity = 56/205 (27.32%), Postives = 80/205 (39.02%), Query Frame = 3
Query: 2232 CLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDT------GKCLCREGVEGQHCDTCKPGYWNFPF-----CHSCKCNQMST-----TCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI-LCKKCQCP---GLSSLKNNSIG--------------CSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAI 2744
C N G C+ C ++ Y ++ L + N C G+C C+ V G+ CD C PG+W F C +C CN T CD TG+C CK + G C C +G++ ++G C CQC G S +S G + + A +C C+KG G C Q F A+
Sbjct: 366 CQHNTQGDHCQNCTAKHYRDLSKPINHVNACLGITNNGSCLTQATDTEQIGQCSCKPNVYGRRCDQCIPGHWGFTLPPQGECKACNCNISGTRNATINCDQLTGQCF-CKPSIQGVYCDGCRDGFHTFPTQGDGGCAPCQCDLGGGFSICDKSSDGICTQKCVEYTLPTYIHISIASNAGSCTCRKGVEGFSCKQALYGTFYPAL 569 HSP 9 Score: 58.151 bits (139), Expect = 3.928e-8 Identity = 49/158 (31.01%), Postives = 68/158 (43.04%), Query Frame = 3
Query: 2829 ECRKCLFNTSGFNCEKCVSGY----FGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCP-------------CLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGAL---NHSCNQLTGQCYCQAGR--SGKMCNNCKDGFWGDPTKLNGCTKC 3236
E +C N +G +CE C GY G C C CN C+ ETG C C NV G +C RC+ +++ LS GC C+C+ P G L C+Q TGQC C A G++C+ C++ + L C +C
Sbjct: 1072 ERAECHRNYTGLSCEVCAEGYTRQPMGGTPFDRCVLCSCNDRSR------FCHPETGVCTDCKIGTHGYRCELCTNNVQGPECVRCKPQYWGLSKD-GCKACDCNLP-GTLYSNTSVCDQTTGQCSCNATLHIGGQLCDRCEENAYNKSDSLFDCHEC 1221 HSP 10 Score: 53.5286 bits (127), Expect = 9.391e-7 Identity = 43/140 (30.71%), Postives = 56/140 (40.00%), Query Frame = 3
Query: 2376 CREGVEGQHCDTCKPGYWNFPF-------CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENG------------YYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGY--SGQFCDQCETNHF 2732
C G C+ C GY P C C CN S C +TG C +CK + GY C+ C N Y+G + G CK C C +L +N+ C + T C C GQ CD+CE N +
Sbjct: 1076 CHRNYTGLSCEVCAEGYTRQPMGGTPFDRCVLCSCNDRSRFCHPETGVCTDCKIGTHGYRCELCTNNVQGPECVRCKPQYWGLSKDG--CKACDCNLPGTLYSNTSVC----DQTTGQCSCNATLHIGGQLCDRCEENAY 1209 HSP 11 Score: 53.1434 bits (126), Expect = 1.209e-6 Identity = 42/127 (33.07%), Postives = 57/127 (44.88%), Query Frame = 3
Query: 1308 LNAGHCICKENVEGNQCDKCKIGFWKFNGNEET--GCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIK 1682
+N+ + + N E QC F+ + E G C C G+ C + G+C CK V G C+ C GY+ LS++ GC C+CS GS CD GKC CK G +CD K
Sbjct: 729 VNSRIAMSRINTENPQCRA-----LVFSASTELYNGTKVCECDPEGSTNPTQCVAYGGQCPCKPGVAGLKCNHCLPGYYALSSS---GCKKCECSMSGS-ITPVCDNADGKCQCKANVEGAKCDACK 846 HSP 12 Score: 50.447 bits (119), Expect = 9.273e-6 Identity = 73/318 (22.96%), Postives = 114/318 (35.85%), Query Frame = 3
Query: 618 TSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPW--LPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG--CDSSTGKCYCKS--FVTGNDCSTCKIGYFNLSNA 1553
S+ ++I+ S V AD + V ++ ++T + Q + + G W IR Y+ + +R + G A KA+ G +V V +C N G CE C + Y P P D+ C C CN S CH + GVC +C+ T CE +C NV+G +C +CK +W G + GC C+C + GTL + CD +TG+C C + + G C C+ +N S++
Sbjct: 983 ASKMTAILLSGNRNVTLQADVT---TGHVTLDETHMVTPSLTPYQFQSLLTNLSGLW-------IR-ALYHPHPDGSVRFQGVSLGSA---KARGGG-EVAGFVERA-ECHRNYTGLSCEVCAEGYTRQPMGGTPFDR-----CVLCSCNDRSRFCHPET-----------GVCTDCKIGTHGYRCE---------------------------------------------------LCTNNVQGPECVRCKPQYW---GLSKDGCKACDCNLPGTLYSNTSVCDQTTGQCSCNATLHIGGQLCDRCEENAYNKSDS 1214
BLAST of Netrin-4 vs. Ensembl Medaka
Match: lamb2 (laminin subunit beta 2 [Source:NCBI gene;Acc:101158524]) HSP 1 Score: 857.44 bits (2214), Expect = 0.000e+0 Identity = 551/1799 (30.63%), Postives = 871/1799 (48.42%), Query Frame = 3
Query: 66 TLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTYPLE-------------------HLKEIKINLPPVSKSGYYNFVITTDTKNMQF-----FDSFI------IVLSIYHVGK----SSC-QSYQDTMQLI----KDSRYIVIPNK-CLEKDLPIVLTV-FT---------IESNNKXXXXXXXXXXXXXXXXN-------STKTRKVLGE---CLNYANLVSSKKCDFLNAEI--STNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT----SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTN-SKWQG----ICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFL----IVVKNTFDKLH--NSSGVFMSNVKFPKEIDD--------------VCTSLEQNMKSLKEIKKYFPIGYD----------IIISNFSEKLSRLKYYVYNSNIIDFNTQQ--NSIITIENST---DYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQF----NISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYLNG-SADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFL--KLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNL--KIDTKVVVNKSNYFKLKSASNSK-ISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKI 5090
+L + + A E + + C C P GDLL+GRE L+ASSTCG+ E YC+V+ + KC CDS+ PY +T +HRIENV++ P WWQS +G +V I++ E+EF FT + FKT+RPAAM IERS D+GRTW Y YFS +C FP + NIN+V+C S +S I PS G V++ D Y +N + ITN+RV F +LHT GD +D I+ KYYYAI E ++RG C CYGHAS+C +G + ++ MV+G C C HNTKG +CE+C DFYND PW PA+ +TN C+KC CN HS+ CHFD VY + +SGGVCD CQHNT +NCE C+ YY++ I C C CD G++ +C +L LG+ G C CK NVEG +CDKCK GF+ + + GC C C GT+ CD +G C+CK VTG C C ++ LS+ ++NGC C C G+ DN C SG+C C+ G +C +++S ++ ++ Y E H + V + G F I+ +M++ ++S + + +S+ G S C + D +L+ +R++V P CLE+D+ L FT I +N I ++ ++ ++ RK E C A V + A++ S + + + C C+ GS ++ C+ GGQC+C PNV G+ C++CA +YGF GC C C L GS+ C +G+C CR G GQ CD C+ G+W FP C C+CN + CD +TG C+NC+ N+ G C C +GYYG + G C+ C CP G +S ++ + C C C +GY+G C++C ++GN G CQPC+C+ NID D+C+ +TGECRKCL+NT G NC C SGY G+ A R C KC CN +GT S+ G +C +G+C CLPNVVGL CD C +NL++ +GC PCNCD P ++ SCN+ TGQC C+ G G+ C +C++ FWGDP + C C C+ G QC+R G C C+ G++G CD C +G G+ P+C C C+ WD + + + ++ H ++G+ KF K++++ ++ M+ +++++ D ++ + SE L + ++ ++Q + + ++NS Y S N+ RD NE + + ++ ++++++ + + K F R L + K +D+D + N C + + +P C E CGG GC+ D RH C G + A L + + KE N+ + E K + +S+ + T+ ++ + +L+ +I+ +L AD E++ + + I ++I L +I++R+R L+NV IL +++ + ++ + A RA+ + ++ + ++ Q + ++ + + + L + E+E + +++ +R+ L ++ I+K +N + N +I + +K N + + + +K + E + ND +K + KL K + + EL + + ++ K +L L+ K+
Sbjct: 4 SLFTLVSVLAAAVGQEPDHAHGCAHGSCYPATGDLLVGREKDLKASSTCGMRRKEPYCIVSHLQD--EKKCFECDSRRPYNPVYNTINHRIENVIATFKPHRKNSWWQSENGKSDVFIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRTWQVYRYFSHDCDASFPGVSHGPLRNINDVICESRYSDIEPSTEGEVIYRVLDPSIRIEDPYSPAIQNQLK-ITNLRVNFTKLHTLGDNLLDPRAEIKEKYYYAIYELVVRGNCFCYGHASECAPIKGVRADIEGMVHGRCVCNHNTKGLNCEQCDDFYNDRPWRPAEGRNTNACKKCNCNDHSNLCHFDMAVYLATGNVSGGVCDECQHNTMGRNCEMCQLFYYKDPLKDIRDPHVCKPCDCDPDGSENGGMCDSHTDLALGMMGGQCRCKANVEGPRCDKCKTGFFGLSADSAHGCQPCRCDRRGTVTGSSQCDPVSGDCFCKRLVTGRSCDQCLPEHWGLSH-DLNGCRGCDCDVGGALDN-LCSMESGQCRCRGHMTGRQCTQVESGYFFMALDHFLYEAEFAKMGQGCTLETREHQSGRHASWTDVGFARVPEGGTLEFTISNIPYSMEYDLLIRYESQMPRDWEEVQISVIRPGPIPTSSPCGNTIPDDDRLLVSLPAGARFVVPPQPVCLERDVIYTLRFEFTRYQDANSILIGGSNAILLMDSIALMPRHSSLDMFTAGDPASAVRKQTYERYHCHEGAKSVIRPQMSDTCAKLISSMSAIINDGALACNCDPQGSMSSVCDVRGGQCRCRPNVIGQRCDKCAPGTYGFGPSGCIPCGCSLEGSVTRLCDKFSGQCQCRPGAFGQRCDGCQAGHWGFPSCRPCQCNGHADQCDQRTGACINCRDNTGGDKCDRCADGYYGSPVLGGASGAQCRPCPCPEGPNSGRHFAASCYQDNRNLQIICNCNQGYTGARCEECAPGYYGNPSQPGGRCQPCQCNNNIDMSDMDACDRRTGECRKCLYNTEGPNCNICRSGYHGDAARRNCRKCTCNFLGTERSQCTGREDCVCQRASGQCQCLPNVVGLTCDHCAPNHWNLANGMGCAPCNCD-PNNSVTSSCNEFTGQCQCRDGFGGQTCRDCQENFWGDPRTI--CRACDCDWRGIETSQCHRATGHCVCQQGVSGVRCDQCARGFSGQFPDCQPCHQCFGDWDRIVQDLAVRTRALAERAHEIQTTGLIGPYEKFFKDLEEKLARAQGIVNERNATAAAVSALMELIEDLRAQIGETTDTLNRLEGDLTLVQDSNSEASKELSALEREAKELNLTSEQLHHQLDVLKNSNFLGAYDSIRSSHNRSRDAERRANESTTTRPSTVSQSADTRRRTERLIAAKKDDFNRKNAANKRGLGDLNAKVQDLD---------MTRLNEKVCGS-SSHDP--CGESPCGGAGCRDDDGNRH------CGGLNCDGAVAAADDALQKAKHA--EKELNKALEETQGLFTKVAEAKSKAEEAKSKAQAALDKATATKNKVERSNNDLRDLIKQIRNFLTQEGADPDSIEAVANQVLRLSIPASPSQIRELADEIKARVRSLSNVEAILQQTQEDVRKAEQLLLDAKRARHQAEGVKSTAEAVTQALLDAQTAQTSAEKAIAKAKTDIGEARNRLTQI---ESETSDNEKDLNDAMDRLGTLGREINILKTKCAENNMAAARAEETATMARDKANEAKQILDGQLTDKYNEVQQRVDTKAKAVQEAKEKAETFREEANDLLKDAQDKL----KRLAELELSYEENQRKLEGKARQLDGLEGKM 1767
BLAST of Netrin-4 vs. Ensembl Medaka
Match: lamb2 (laminin subunit beta 2 [Source:NCBI gene;Acc:101158524]) HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0 Identity = 551/1811 (30.43%), Postives = 871/1811 (48.09%), Query Frame = 3
Query: 66 TLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRL-PQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEG-QVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTYPLE-------------------HLKEIKINLPPVSKSGYYNFVITTDTKNMQF-----FDSFI------IVLSIYHVGK----SSC-QSYQDTMQLI----KDSRYIVIPNK-CLEKDLPIVLTV-FT---------IESNNKXXXXXXXXXXXXXXXXN-------STKTRKVLGE---CLNYANLVSSKKCDFLNAEI--STNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT----SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSG------------QFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTN-SKWQG----ICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFL----IVVKNTFDKLH--NSSGVFMSNVKFPKEIDD--------------VCTSLEQNMKSLKEIKKYFPIGYD----------IIISNFSEKLSRLKYYVYNSNIIDFNTQQ--NSIITIENST---DYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEF-CGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQF----NISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYLNG-SADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFL--KLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNL--KIDTKVVVNKSNYFKLKSASNSK-ISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKI 5090
+L + + A E + + C C P GDLL+GRE L+ASSTCG+ E YC+V+ + KC CDS+ PY +T +HRIENV++ P WWQS +G +V I++ E+EF FT + FKT+RPAAM IERS D+GRTW Y YFS +C FP + NIN+V+C S +S I PS G V++ D Y +N + ITN+RV F +LHT GD +D I+ KYYYAI E ++RG C CYGHAS+C +G + ++ MV+G C C HNTKG +CE+C DFYND PW PA+ +TN C+KC CN HS+ CHFD VY + +SGGVCD CQHNT +NCE C+ YY++ I C C CD G++ +C +L LG+ G C CK NVEG +CDKCK GF+ + + GC C C GT+ CD +G C+CK VTG C C ++ LS+ ++NGC C C G+ DN C SG+C C+ G +C +++S ++ ++ Y E H + V + G F I+ +M++ ++S + + +S+ G S C + D +L+ +R++V P CLE+D+ L FT I +N I ++ ++ ++ RK E C A V + A++ S + + + C C+ GS ++ C+ GGQC+C PNV G+ C++CA +YGF GC C C L GS+ C +G+C CR G GQ CD C+ G+W FP C C+CN + CD +TG C+NC+ N+ G C C +GYYG + G C+ C CP G +S ++ + C C C +GY+G C++C ++GN G CQPC+C+ NID D+C+ +TGECRKCL+NT G NC C SGY G+ A R C KC CN +GT S+ G +C +G+C CLPNVVGL CD C +NL++ +GC PCNCD P ++ SCN+ TGQC C+ G G+ C +C++ FWGDP + C C C+ G QC+R G C C+ G++G CD C +G G+ P+C C C+ WD + + + ++ H ++G+ KF K++++ ++ M+ +++++ D ++ + SE L + ++ ++Q + + ++NS Y S N+ RD NE + + ++ ++++++ + + K F R L + K +D+D + N C + + +P C E CGG GC+ D RH C G + A L + + KE N+ + E K + +S+ + T+ ++ + +L+ +I+ +L AD E++ + + I ++I L +I++R+R L+NV IL +++ + ++ + A RA+ + ++ + ++ Q + ++ + + + L + E+E + +++ +R+ L ++ I+K +N + N +I + +K N + + + +K + E + ND +K + KL K + + EL + + ++ K +L L+ K+
Sbjct: 4 SLFTLVSVLAAAVGQEPDHAHGCAHGSCYPATGDLLVGREKDLKASSTCGMRRKEPYCIVSHLQD--EKKCFECDSRRPYNPVYNTINHRIENVIATFKPHRKNSWWQSENGKSDVFIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRTWQVYRYFSHDCDASFPGVSHGPLRNINDVICESRYSDIEPSTEGEVIYRVLDPSIRIEDPYSPAIQNQLK-ITNLRVNFTKLHTLGDNLLDPRAEIKEKYYYAIYELVVRGNCFCYGHASECAPIKGVRADIEGMVHGRCVCNHNTKGLNCEQCDDFYNDRPWRPAEGRNTNACKKCNCNDHSNLCHFDMAVYLATGNVSGGVCDECQHNTMGRNCEMCQLFYYKDPLKDIRDPHVCKPCDCDPDGSENGGMCDSHTDLALGMMGGQCRCKANVEGPRCDKCKTGFFGLSADSAHGCQPCRCDRRGTVTGSSQCDPVSGDCFCKRLVTGRSCDQCLPEHWGLSH-DLNGCRGCDCDVGGALDN-LCSMESGQCRCRGHMTGRQCTQVESGYFFMALDHFLYEAEFAKMGQGCTLETREHQSGRHASWTDVGFARVPEGGTLEFTISNIPYSMEYDLLIRYESQMPRDWEEVQISVIRPGPIPTSSPCGNTIPDDDRLLVSLPAGARFVVPPQPVCLERDVIYTLRFEFTRYQDANSILIGGSNAILLMDSIALMPRHSSLDMFTAGDPASAVRKQTYERYHCHEGAKSVIRPQMSDTCAKLISSMSAIINDGALACNCDPQGSMSSVCDVRGGQCRCRPNVIGQRCDKCAPGTYGFGPSGCIPCGCSLEGSVTRLCDKFSGQCQCRPGAFGQRCDGCQAGHWGFPSCRPCQCNGHADQCDQRTGACINCRDNTGGDKCDRCADGYYGSPVLGGASGAQCRPCPCPEGPNSGRHFAASCYQDNRNLQIICNCNQGYTGIVKRSGATIFKRARCEECAPGYYGNPSQPGGRCQPCQCNNNIDMSDMDACDRRTGECRKCLYNTEGPNCNICRSGYHGDAARRNCRKCTCNFLGTERSQCTGREDCVCQRASGQCQCLPNVVGLTCDHCAPNHWNLANGMGCAPCNCD-PNNSVTSSCNEFTGQCQCRDGFGGQTCRDCQENFWGDPRTI--CRACDCDWRGIETSQCHRATGHCVCQQGVSGVRCDQCARGFSGQFPDCQPCHQCFGDWDRIVQDLAVRTRALAERAHEIQTTGLIGPYEKFFKDLEEKLARAQGIVNERNATAAAVSALMELIEDLRAQIGETTDTLNRLEGDLTLVQDSNSEASKELSALEREAKELNLTSEQLHHQLDVLKNSNFLGAYDSIRSSHNRSRDAERRANESTTTRPSTVSQSADTRRRTERLIAAKKDDFNRKNAANKRGLGDLNAKVQDLD---------MTRLNEKVCGS-SSHDP--CGESPCGGAGCRDDDGNRH------CGGLNCDGAVAAADDALQKAKHA--EKELNKALEETQGLFTKVAEAKSKAEEAKSKAQAALDKATATKNKVERSNNDLRDLIKQIRNFLTQEGADPDSIEAVANQVLRLSIPASPSQIRELADEIKARVRSLSNVEAILQQTQEDVRKAEQLLLDAKRARHQAEGVKSTAEAVTQALLDAQTAQTSAEKAIAKAKTDIGEARNRLTQI---ESETSDNEKDLNDAMDRLGTLGREINILKTKCAENNMAAARAEETATMARDKANEAKQILDGQLTDKYNEVQQRVDTKAKAVQEAKEKAETFREEANDLLKDAQDKL----KRLAELELSYEENQRKLEGKARQLDGLEGKM 1779
BLAST of Netrin-4 vs. Ensembl Medaka
Match: lamb1b (laminin subunit beta-1 [Source:NCBI gene;Acc:101165682]) HSP 1 Score: 776.933 bits (2005), Expect = 0.000e+0 Identity = 450/1311 (34.32%), Postives = 686/1311 (52.33%), Query Frame = 3
Query: 78 VFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE-MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRG-CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTYPLEHLKE--------------------------------IKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLS--IY-------HVGKSSCQSYQDTMQLIKDSRYIVIPNK-CLEKDL----PIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGE----------CLNYA-NLVSSKKCDFLNAEI-STNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYG--DTSRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTN----SKWQG-ICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEI--KKYFPIGYDIIISNFSEKLSRLKYY--VYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNG 3782
+ ++ + E E +VC + C P GDLLIGR L ASSTCG++ R+C+V + + KC +CDS+ Y ++ SH IENVV+ + + WWQS +GV NV+I++ E+EF FT + FKT+RPAAM IERS D+G++W Y YF+ +C FP I +++++C + +S I PS+ G V+F + D Y + +N + ITN+RV+F++LHT GD +D + KYYYA+ + ++RG C CYGHAS+C +G+ E MV+G C C HNT+G +CE+C+DFY D PW PA +T+ C++C+CN H++SCHFD V+ + +SGGVCDNCQHNT + CE+C +Y++ + C C CD G+ C ++ GL +G C CK NVEG +CD C+ G++ G GCL C C +GT+ CDS TGKC CK V G++C C ++ LSN +++GC C C G+ N CD SG+C+C+ G RCD+++S +Y ++ TY E K ++ N+ + +S Y+ +I + + Q ++ ++ + ++ H S + ++ L SR++++ C EK L + L +++ S+ + P ++ID +V+ GE CL + N++ S D I S + + + C+C+ GS ++ C GGQC+C PNV G+ C+ CA ++ F GC+ C CD GS+ + C+ TG+C C G G+ C C PG+W FP C C+CN S C TG+C C+ + G++C+ C NGY+G + G C+ C CP G S + S C L+ ++ C+C GY G CD+C ++GN + G C C+C+ NID SC+S+TG C KCL++T G C+ C GY+GN A ++CTKC+C+ GT S +G C+ +G+CPC PNVVG CD C + +SS GC C+CD P+ ++ SC+++TGQC C+ G G+ C CK+ FWGDP C C C+ G QC+R G C C +G CD C +G G+ P C C C+ WD + + N +L + + ++ D+ +SLE N K+++EI K + I + + + + Y V NS Q E + D A + + EL++ + NI N
Sbjct: 10 AWALWCPVWTQEPEFADVCTEGSCYPATGDLLIGRAHKLSASSTCGVSQPTRFCIVGHLEE--EKKCFLCDSRQMYDEETNNISHTIENVVTTFAPNRLKTWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMMIERSMDFGKSWQIYRYFAYDCETSFPGISTGPMVRVDDIICDTRYSDIEPSKEGEVIFRVLDPAYDIEDPYSLRIQN-MLKITNLRVKFIKLHTLGDNLLDSRDEVTEKYYYALYDMVVRGNCFCYGHASECAPMDGEPSGAEGMVHGRCMCNHNTEGLNCERCQDFYQDLPWRPAQGKNTHACKRCDCNHHATSCHFDLAVFMMTGNVSGGVCDNCQHNTMGRQCEQCARFFYQHPNRHLRDPNICEPCDCDPDGSVEGGNCDPRTDVAAGLISGQCRCKANVEGERCDNCRRGYYGL-GKHPEGCLPCTCNQLGTMPGGAHCDSETGKCICKRLVDGHNCDQCLPEHWGLSN-DMDGCRPCDCDHGGAVHN-NCDPDSGQCVCREHMFGRRCDQVESGFYFIDLDHYTYEAEDAKHGPGVAVVPRPYPLNRSPTWTGMGFVNVPEGAYLEFNVNNIPESMDYDILIRYEPQLPQQWEQLVLRVERPVFNPPNRSGHCTNSIQSGDEQSISLSPGSRHVLLVRPVCFEKGLNYTIRLTLPLYSTNSHVQNPYTLIDSLVLMPRVRELEMFHGTKGEAEWDTFQRYRCLESSRNVIKSPMTDICKDYIFSVSALLHKGAMACQCDPQGSLSSVCESSGGQCRCRPNVVGRNCDHCAPGTFLFSPAGCRPCNCDPLGSVRAFCHELTGQCECVRGATGRQCSQCLPGFWGFPQCRMCQCNGHSDYCHPNTGECQGCRDFTTGHHCERCMNGYHGNPELGSGEHCRPCMCPDGPRSGRQFSDSCYLLADSDHLVCVCSSGYKGSRCDECAPGYYGNPLVPGGRCMACQCNNNIDMHDPKSCDSRTGACLKCLYHTEGHACQHCKPGYYGNAATQSCTKCMCSPFGTEQEACSSAEGCQCDRRSGQCPCQPNVVGQNCDHCAPDTWGISSGKGCQRCDCD-PVHSVGSSCDEVTGQCVCKPGFGGRTCRECKELFWGDPKV--KCHACDCDPRGISSGQCHRATGHCACLESASGPRCDTCARGHWGEFPACEPCHRCFPVWDTVVSELANQTRRLQ-AQVTELHAIRVTAPYKDLISSLESNAKAVREILENKTASAKMEKIAEDSHQIIGLMSYLSGVMNSTEETLTFLQGDANMTEANLDALTAEADQ-----LDANVQELRRLVHNIKNA 1305
BLAST of Netrin-4 vs. Ensembl Medaka
Match: lamb1a (laminin subunit beta 1 [Source:NCBI gene;Acc:101167953]) HSP 1 Score: 463.766 bits (1192), Expect = 6.247e-135 Identity = 236/545 (43.30%), Postives = 336/545 (61.65%), Query Frame = 3
Query: 117 ESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQ-RFSSTHSHRIENVVSRLPQDPFR-WWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE-MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY---IEKPTYPLEHLK 1730
E +VC + C P GDLLIGR L A+STCG+T E +C+V S + KC +CDS P++ R + T+SHR+ENVV+ + + WWQS +G+ NV+I+++ E+EF FT + FKT+RPAAM IERS D+G+TW Y YF+ +C FPS+P+ +++V+C S +S I PS G V++ + +D Y + +N + ITN+RV+F +LHT GD +D I+ KYYYA+ + ++RG C CYGHAS+C G Q E MV+G C C HNTKG +CE+C+DFY+D PW PA+ +TN C++C CN HS +CHFD V+ S +SGGVCDNCQHNT CE+CK +Y++ C C+CD LG+ +C ++ GL AG C CK NVEG +CD+C+ + + +E GC C C +GT CDS +G C+CK TG DC C ++ LSN +++GC C C G+ N+ CD+ SG+C+C++ G RCD+I+S +Y ++ TY E K
Sbjct: 52 EFSDVCTEGSCYPATGDLLIGRAHQLFATSTCGVTRPEPFCIV--SHLQEEKKCFLCDSTVPFEERANHTNSHRVENVVTTFGPNRLKTWWQSENGLENVTIQLNLEAEFHFTHLIMTFKTFRPAAMVIERSADFGKTWQVYRYFAYDCESAFPSVPQGPMQKVDDVICDSRYSDIEPSTEGEVIYRVLDPAFRIDDPYSPRIQN-LLKITNLRVKFTKLHTLGDNLLDSRIEIKEKYYYAVYDMVVRGNCFCYGHASECAPVLGAGQTMEGMVHGHCMCNHNTKGLNCEQCQDFYHDLPWRPAEGRNTNACKRCNCNQHSDACHFDMAVFVASGGVSGGVCDNCQHNTAGHKCEQCKPFFYQHPERDARDPNICQPCNCDPLGSVNGGLCDGMTDIRAGLIAGQCRCKVNVEGERCDRCRPAHYGLS-DEPEGCKACTCSPLGTAPGGNPCDSQSGNCFCKRLTTGQDCDQCVPEHWGLSN-DVDGCRPCDCDLGGAIHNQ-CDQMSGQCVCRDHMFGRRCDQIESGFYFSTLDHYTYEAEDAK 590 HSP 2 Score: 399.438 bits (1025), Expect = 3.003e-113 Identity = 290/1028 (28.21%), Postives = 486/1028 (47.28%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCP-GLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGI-----CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHN------SSGVFMSNVKF----PKEIDDVCTSLEQNMKS-LKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILN----GASESKKIY-----------QNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISIN-----SKEIKDAYVKP-ESEYNTTIKLIDVTRGAIQSRRI--ESVNLLIKIKTYL-NGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNN----ISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNK-SNYFKLKSASNSKISERFQSLLNKSHNL----NDKIKA----------SHYKLKLNKKLIVDKELKLQSLSKDIDEKLS 5063
+C+C+ GS + C+ GGQC+C PNV G++C+RCA ++ F GC C+C+ GS + C TG+C C G G+ CD C PG+W FP C C CN + TCD TG CL+C+ ++ G++C+ C +GYYGD G C+ C CP G SL+ + C ++ C+C GY G CD+C +G+ G C PCRC+ NID +SC+ ++GEC +CL+ + G C+ C GY+GN ++ C KCVCN +GT+ CN +G+CPCLPNV G CDRC +N++S +GC C+CD P + SCNQ++GQC C G G+ CN CK+ FWGDP C C C+ G +QCN+ NG C C G++G CD C +G G P+C C C+ +WD + + N +L N +SGV + KF K ++ L+QN+ S + ++ + S+ L+R + ++ QNS+ E +++S + D T EL+ ++ I N GAS+S Y T+ + EL + L + Q ++ + E ++N+ G T+ S + + CGG D C R + C GK + N+ + ++E ++ ++ SK + +A ++ E++ + L + R ++ R E +L+ I+ +L +AD E + + ++ ++ L ++IR ++ +L NV +IL++S N ++ + + A A+E+ + + +S + + + QK + + + A + + K L + A K NN + + E + LK + + + T Q I Q + L+ K V+ K+ ++ +R +SL ++ L DK++ + + L++ + +V+ E ++ L +DI K+S
Sbjct: 794 ECQCDPQGSLSTVCDPNGGQCRCRPNVVGRSCDRCAPATFQFGPSGCIACECNPLGSQSPFCDQLTGQCGCVVGAYGRQCDRCLPGHWGFPACRPCSCNGHAETCDPDTGSCLHCRDHTAGHSCERCLDGYYGDPVLGSGDHCRPCMCPDGPESLRQFAGSCFRSDDSQQAVCVCSTGYRGPRCDECSPGFYGSPSEVGGQCVPCRCNNNIDMLDPESCDRQSGECLRCLYYSEGTACQDCKLGYYGNALLQDCRKCVCNKLGTSPSSCSSSGDCHCNRSSGQCPCLPNVTGQYCDRCAPDTWNMASGLGCQSCDCD-PKHSYGTSCNQISGQCSCHPGFGGRTCNECKELFWGDPEV--KCYACDCDPRGISEQQCNKANGRCVCVEGVSGPRCDTCARGFSGVFPDCQRCHQCFAEWDDVIGQLTNQTHRLVNKVNALRTSGVSGPHKKFFDSMQKSAAEIQVVLDQNLASQPLTELQQLLQQASDLMGSLSQTLNRTE-----QTFVELE-DQNSVTKTE-----VDSLMSDTQKLDET--VQELQLQVQFIKNSNIRGASDSITKYFLKSQAAEARANASTVDSGSPTELSAELHRLTLDTLNQTQEEFMSRHTEHAQNLDQLAG-ELQTLDLS--WINQKMCGGPSNAQDSC-RSSCGGLGCEGKGGCGGEGCDGAVTTANNVLMRVEESEREIASALEEVDQLSKMVLEAKLQADEAKLSAQNVLNNANRTKLKVDRSNEELRSLIRHIRDFLTQDAADPDSIEHVANEVLALQMPTTPAQLQNLTEEIRLKVGELGNVESILEQSAENIQRAEALLEQARHAREEAAGVKDSAEKVKHVLEEVQKAQTSVGSAIQQAAAEIQNTKELLSSVEIETASAELKVNNATVRLQRLDEDVMLLKSKNWNVSRSANMTSQDAAIIQSMADEVKQELQSGLKQKVSTVEQLIDQKAVGVAQAKDRAESLQKEAKELLLQAADKLQLLKDLEKSYEDNQHILEVKAEQLVELEAAVKELLQDISHKVS 1801 HSP 3 Score: 97.0561 bits (240), Expect = 1.454e-19 Identity = 74/251 (29.48%), Postives = 115/251 (45.82%), Query Frame = 3
Query: 2673 CMCKKGYSGQFCDQCET-NHFGNAISKDG----TCQPCRCSGNIDQRIED------SCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGA-----VRTCTKCVCNHMGTNSKWQGICNHET--------GKCPCLPNVVGLQCDRCQDKFFNLSSQI-GCLPCNCDYPIGAL--NHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKG 3344
CMC G C+QC+ H +G C+ C C+ + D D S G C C NT+G CE+C ++ + C C C+ +G+ + G+C+ T G+C C NV G +CDRC+ + LS + GC C C P+G + C+ +G C+C+ +G+ C+ C WG ++GC C C+L G++ QC++ +G C C+ + GR CD G
Sbjct: 326 CMCNHNTKGLNCEQCQDFYHDLPWRPAEGRNTNACKRCNCNQHSDACHFDMAVFVASGGVSGGVCDNCQHNTAGHKCEQCKPFFYQHPERDARDPNICQPCNCDPLGSVNG--GLCDGMTDIRAGLIAGQCRCKVNVEGERCDRCRPAHYGLSDEPEGCKACTCS-PLGTAPGGNPCDSQSGNCFCKRLTTGQDCDQCVPEHWGLSNDVDGCRPCDCDLGGAIHNQCDQMSGQCVCRDHMFGRRCDQIESG 573 HSP 4 Score: 95.1301 bits (235), Expect = 5.994e-19 Identity = 75/266 (28.20%), Postives = 118/266 (44.36%), Query Frame = 3
Query: 2493 GKCLNCKGNSFGYNCQNCENGYYGDTSRGI------LCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISK---------------DGTCQPCRC-------SGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFG-NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQI-GCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFW 3200
G C+ C N+ G NC+ C++ Y+ R CK+C C +S C F+ F + G SG CD C+ N G+ + CQPC C G D + G+CR C N G C++C ++G + C C C+ +GT + C+ ++G C C G CD+C + + LS+ + GC PC+CD GA+++ C+Q++GQC C+ G+ C+ + GF+
Sbjct: 324 GHCM-CNHNTKGLNCEQCQDFYHDLPWRPAEGRNTNACKRCNC------NQHSDACH--FDMAVF--VASGGVSGGVCDNCQHNTAGHKCEQCKPFFYQHPERDARDPNICQPCNCDPLGSVNGGLCDGMTDIRAGLIAGQCR-CKVNVEGERCDRCRPAHYGLSDEPEGCKACTCSPLGT-APGGNPCDSQSGNCFCKRLTTGQDCDQCVPEHWGLSNDVDGCRPCDCDLG-GAIHNQCDQMSGQCVCRDHMFGRRCDQIESGFY 575 HSP 5 Score: 91.6633 bits (226), Expect = 7.391e-18 Identity = 72/261 (27.59%), Postives = 115/261 (44.06%), Query Frame = 3
Query: 2364 GKCLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTCD-----------SKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKNN------SIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFG-NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFF 3050
G C+C +G +C+ C+ Y + P+ C C CNQ S C G C NC+ N+ G+ C+ C+ +Y R +C+ C C L S+ I LI A C CK G+ CD+C H+G + +G C+ C CS + C+S++G C C T+G +C++CV ++G + V C C C+ G C+ +G+C C ++ G +CD+ + F+
Sbjct: 324 GHCMCNHNTKGLNCEQCQDFYHDLPWRPAEGRNTNACKRCNCNQHSDACHFDMAVFVASGGVSGGVCDNCQHNTAGHKCEQCKPFFYQHPERDARDPNICQPCNCDPLGSVNGGLCDGMTDIRAGLI----AGQCRCKVNVEGERCDRCRPAHYGLSDEPEG-CKACTCSPLGTAPGGNPCDSQSGNCF-CKRLTTGQDCDQCVPEHWGLSNDVDGCRPCDCDLGGA---IHNQCDQMSGQCVCRDHMFGRRCDQIESGFY 575 HSP 6 Score: 91.2781 bits (225), Expect = 9.125e-18 Identity = 76/279 (27.24%), Postives = 116/279 (41.58%), Query Frame = 3
Query: 879 ASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPW-LPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHC----DSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNC-VMMGTLENRGCDSSTGKCY-CKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKT-------CDKYSGKCLCKNGFAGIRCD 1673
A +C Q + + G C C G+ C++C + W PA CR C CN H+ +C D G C +C+ +T +CERC GYY + + S C C C +SL +F C + + + C+C G +CD+C GF+ C+ C C + L+ CD +G+C C + G C CK+GY+ NA + C C C++ G+ + C++ SG+C C G CD
Sbjct: 842 ACECNPLGSQSPFCDQLTGQCGCVVGAYGRQCDRCLPGH----WGFPA-------CRPCSCNGHAETCDPD-----------TGSCLHCRDHTAGHSCERCLDGYYGD--PVLGSGDHCRPCMCPDGPESL-RQFAGSCFRSDDSQQAV----CVCSTGYRGPRCDECSPGFYGSPSEVGGQCVPCRCNNNIDMLDPESCDRQSGECLRCLYYSEGTACQDCKLGYYG--NALLQDCRKCVCNKLGTSPSSCSSSGDCHCNRSSGQCPCLPNVTGQYCD 1089 HSP 7 Score: 87.0409 bits (214), Expect = 1.602e-16 Identity = 72/247 (29.15%), Postives = 104/247 (42.11%), Query Frame = 3
Query: 933 GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCD 1673
VC C +G C++C + SP +C C CN++ DP +S G C C + +E C+ CK GYY N + Q C KC C+ LGT S + ++G C C NV G CD+C W N GC C+C + C+ +G+C C G C+ CK ++ C C C G + + C+K +G+C+C G +G RCD
Sbjct: 964 AVCVCSTGYRGPRCDECSPGFYGSP-----SEVGGQCVPCRCNNNID--MLDP----ESCDRQSGECLRCLYYSEGTACQDCKLGYYGNALL-----QDCRKCVCNKLGTSPSSC-SSSGDCHCNRSSGQCPCLPNVTGQYCDRCAPDTW--NMASGLGCQSCDCDPKHSY-GTSCNQISGQCSCHPGFGGRTCNECKELFWGDPEVK---CYACDCDPRGISEQQ-CNKANGRCVCVEGVSGPRCD 1186 HSP 8 Score: 84.3445 bits (207), Expect = 1.023e-15 Identity = 83/290 (28.62%), Postives = 120/290 (41.38%), Query Frame = 3
Query: 861 CSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCI-CKENVEGNQCDKCKIGFWKFNGNEE-TGCLKCNCVMMGTLENRG-------CDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSE---YGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
CSC GHA C G C+ +T G CE+C D Y P L S + CR C C S +S VC C C+ C G+Y + + C+ C C++ +I + +PE +G C+ C EG C CK+G++ GN C KC C +GT + C+ S+G+C C VTG C C +N+++ GC C C YG+ +C++ SG+C C GF G C+E K ++
Sbjct: 889 CSCNGHAETCDPDTG---------SCLHCRDHTAGHSCERCLDGYYGDPVL----GSGDHCRPCMCPDGPESLRQFAGSCFRSDDSQQAVC-VCSTGYRGPRCDECSPGFYGSPS---EVGGQCVPCRCNN------NIDMLDPE-SCDRQSGECLRCLYYSEGTACQDCKLGYY---GNALLQDCRKCVCNKLGTSPSSCSSSGDCHCNRSSGQCPCLPNVTGQYCDRCAPDTWNMASGL--GCQSCDCDPKHSYGT----SCNQISGQCSCHPGFGGRTCNECKELFW 1145 HSP 9 Score: 82.8037 bits (203), Expect = 3.165e-15 Identity = 74/278 (26.62%), Postives = 114/278 (41.01%), Query Frame = 3
Query: 2181 VTGSRNNNCNKIGGQCQCLPNVSGKTCERCAS--------ESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEG------QH------CDTCKPGYWNFP--------FCHSCKCNQMSTT----CDSKT--------GKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYF 2894
V G+ + G C C N G CE+C + G + CK+C C+ + C+ D + GV G QH C+ CKP ++ P C C C+ + + CD T G+C CK N G C C +YG + CK C C L + + ++ + C CK+ +GQ CDQC H+G + DG C+PC C ++ I + C+ +G+C C + G C++ SG++
Sbjct: 311 VLGAGQTMEGMVHGHCMCNHNTKGLNCEQCQDFYHDLPWRPAEGRNTNACKRCNCN---QHSDACHFDMAVFVASGGVSGGVCDNCQHNTAGHKCEQCKPFFYQHPERDARDPNICQPCNCDPLGSVNGGLCDGMTDIRAGLIAGQC-RCKVNVEGERCDRCRPAHYGLSDEPEGCKACTCSPLGTAPGGN-----PCDSQSGNCFCKRLTTGQDCDQCVPEHWGLSNDVDG-CRPCDC--DLGGAIHNQCDQMSGQC-VCRDHMFGRRCDQIESGFY 575 HSP 10 Score: 81.6481 bits (200), Expect = 7.864e-15 Identity = 50/154 (32.47%), Postives = 70/154 (45.45%), Query Frame = 3
Query: 2169 CKCNVTGSRNNN---------CNKIGGQCQCLPNVSGKTCERCASESYGF--DARGCKKCKCDLTGSL--NSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP----FCHSCKCN---QMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT 2570
C C+ GS N I GQC+C NV G+ C+RC YG + GCK C C G+ + C + +G C C+ GQ CD C P +W C C C+ + CD +G+C+ C+ + FG C E+G+Y T
Sbjct: 426 CNCDPLGSVNGGLCDGMTDIRAGLIAGQCRCKVNVEGERCDRCRPAHYGLSDEPEGCKACTCSPLGTAPGGNPCDSQSGNCFCKRLTTGQDCDQCVPEHWGLSNDVDGCRPCDCDLGGAIHNQCDQMSGQCV-CRDHMFGRRCDQIESGFYFST 578 HSP 11 Score: 71.2478 bits (173), Expect = 1.140e-11 Identity = 62/227 (27.31%), Postives = 93/227 (40.97%), Query Frame = 3
Query: 1044 RKCECNSHSS-SCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKC-YCKSFVTGNDCSTCKIGYF-NLSNANINGCSLCKCS-------EYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
++C+C+ S S DP +GG C C+ N ++C+RC ++ C+ C C+ LG++ P C Q G C C G QCD+C G W F C C+C G E CD TG C +C+ G+ C C GY+ + + + C C C ++ ++ D C+C G+ G RCDE +Y
Sbjct: 793 KECQCDPQGSLSTVCDP---------NGGQC-RCRPNVVGRSCDRCAPATFQF------GPSGCIACECNPLGSQSP-FCDQL--------TGQCGCVVGAYGRQCDRCLPGHWGF-----PACRPCSC--NGHAET--CDPDTGSCLHCRDHTAGHSCERCLDGYYGDPVLGSGDHCRPCMCPDGPESLRQFAGSCFRSDDSQQAVCVCSTGYRGPRCDECSPGFY 985 HSP 12 Score: 70.4774 bits (171), Expect = 1.708e-11 Identity = 62/222 (27.93%), Postives = 94/222 (42.34%), Query Frame = 3
Query: 2841 CLFNTSGFNCEKCVSGYF------GNGA-VRTCTKCVCNH------------MGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIG-----CLPCNCDYPIGALNHS-CNQLT--------GQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVF--KQCNRNNGVCRCKPGITGRNCDMCMK---GTDGKMPNCSSCGDCWL 3392
C NT G NCE+C Y G C +C CN + + G+C++ C N G +C++C+ F+ + C PCNCD P+G++N C+ +T GQC C+ G+ C+ C+ +G + GC C C+ G+ C+ +G C CK TG++CD C+ G + C C DC L
Sbjct: 328 CNHNTKGLNCEQCQDFYHDLPWRPAEGRNTNACKRCNCNQHSDACHFDMAVFVASGGVSGGVCDN------CQHNTAGHKCEQCKPFFYQHPERDARDPNICQPCNCD-PLGSVNGGLCDGMTDIRAGLIAGQCRCKVNVEGERCDRCRPAHYGLSDEPEGCKACTCSPLGTAPGGNPCDSQSGNCFCKRLTTGQDCDQCVPEHWGLSNDVDGCRPC-DCDL 541 HSP 13 Score: 67.3958 bits (163), Expect = 1.806e-10 Identity = 52/188 (27.66%), Postives = 86/188 (45.74%), Query Frame = 3
Query: 1134 DNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCL-CKNGFAGIRCDEIKSEWY 1694
+N Q ++ + C++ +R A+ Q +C CD G+ ++C +P N G C C+ NV G CD+C ++F +GC+ C C +G+ ++ CD TG+C C G C C G++ C C C+ + +TCD +G CL C++ AG C+ +Y
Sbjct: 763 ENSQSVVKTPMTDICRNFIFRISALL---HQGAKECQCDPQGS-LSTVC--DP------NGGQCRCRPNVVGRSCDRCAPATFQFG---PSGCIACECNPLGS-QSPFCDQLTGQCGCVVGAYGRQCDRCLPGHWGFP-----ACRPCSCNGHA----ETCDPDTGSCLHCRDHTAGHSCERCLDGYY 925
BLAST of Netrin-4 vs. Ensembl Medaka
Match: lamb2l (laminin subunit beta-2 [Source:NCBI gene;Acc:101158765]) HSP 1 Score: 449.899 bits (1156), Expect = 4.986e-130 Identity = 233/559 (41.68%), Postives = 325/559 (58.14%), Query Frame = 3
Query: 66 TLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQD-PFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKE--MVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLE-NRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLK 1730
TL+ + A S + C + C P G+LLIGR L A+STCGL E+YC+V S KC CDS+ PY +SHRIENV+ + WWQS SG +VS+ ++ E+EF FT ++FKT+RPAAM IERS D+GRTW Y YF+ NC R FP +P + +IN+V+C +S I PS G V+F D Y + + ++ ITN+R+ F +L+T GD +D ++ KYYYAI E ++RG C CYGHAS+C G V +E M++G C C+HNT+G +CE+CKDF+ D PW PA+ + + C++C CN HS+ CHFD VY + +SGGVCDNC HNT +NCE C+ YY++ + Q CL C CD +G+ +C +LD+G+ AG C CK NV+G +CD CK+G++ + N+ GC CNC G + CD +G C CK +VTG C C Y+ LSN GC C C ++G N C SG+C C+ G +C E++S ++ PL++ K
Sbjct: 7 TLSLLIFALNAAGQELPSSPHGCTEGSCYPATGNLLIGRAVNLTATSTCGLNGPEQYCIV--SHLQELDKCFECDSQRPYHPLRHRNSHRIENVIYLSDRSGELTWWQSASGKEDVSLRLNLEAEFHFTHLIMKFKTFRPAAMIIERSADFGRTWRPYRYFASNCTRTFPGVPAKGLHHINDVICEERYSDIEPSTNGEVIFKVLDPSIPVKDPY-SLEIQELLRITNLRINFTKLNTLGDDLLDRRTDVLQKYYYAIYELVVRGSCFCYGHASECAPVPG-VFTRESGMIHGRCVCKHNTEGLNCERCKDFHKDLPWRPAEAENPHTCKECNCNGHSNQCHFDMAVYLATGNVSGGVCDNCLHNTVGRNCEMCQPFYYQDPNRDVRDPQVCLLCDCDPVGSFDGGVCDSHTDLDMGMIAGQCRCKANVKGIRCDDCKVGYYGLSQNDPQGCQPCNCDPRGIIRMGDPCDQISGDCSCKRYVTGRYCDQCLPEYWGLSNDQF-GCRPCDC-DFGGALNNRCMMESGQCDCRRHLIGRQCSEVQSGYFCA----PLDYYK 555 HSP 2 Score: 407.912 bits (1047), Expect = 5.419e-116 Identity = 277/1058 (26.18%), Postives = 477/1058 (45.09%), Query Frame = 3
Query: 2133 STNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDT--SRGILCKKCQCPG-LSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGT--NSKWQGI--CNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEI----------------------------KKYFPIGYDI------------IISNFSEKLSRLKYYVYNSNI----IDFNTQQNSIITIENSTDYAKAMISKNKI-RDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKS-NQFFNLYASLLLDRTNMSIILKEFNQQF-NISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYLNG-SADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAK---EDTSYLLNSIKN-ISTLNRMTQKFEIIMKQFVMNF---ANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYSKSEFHAQC 5120
S +++ + C+C+ GS ++ CN++GGQC+C PNV G+ C++CA +YGF GC C C GS + QC TG+C CR+G G+ C C+PG W FP C C+CN + CD +TG+C +C+ N+ G+ C+ C+NG+YG+ G C+ C CPG S N C ++ C C +GY+G CDQC ++GN G C PC+C+ NID +SC+ +TG C +CL++T G C +C GY GN + C +C C GT ++ G+ C+ E+G CPC NV G CD+C +N + GC PC+CD P +L CN TGQC+C+ G GK C C+ +WGDP C +C C++ GS + C+R G C C+ G G+ CD C +G G PNC C C+ WD + +K D +H ++ + + P D LE+ +K +K++ + I +I +S ++L L Y V N + + F Q + DY K++ ++ K V+G F+ +++S K +++I + + F + E N +L ++Q++ + + ++S+ V G + ++ S P CGG GC+ D+ + C G+ N + L N L N++ NIS +EI +++ T++ + + + + IK +L G AD + E + + + F + + +IR + L NV IL+++ + A AK E NS K + + +K +K+ N NA ++ L + + + + + N+S E ++ ++ ++ + L + + + ++ K + +S S IS++ + + + + +L +K K +L+ D E ++Q ++++ ++D++ K + + C
Sbjct: 808 SISSIIHDGALACQCDPQGSLSSECNQVGGQCRCKPNVMGRRCDQCAPGTYGFGVSGCTACNCHPDGSSSHQCDPLTGQCPCRQGAIGRQCSDCQPGQWGFPSCRRCQCNGHADLCDPQTGECRDCRDNAAGHQCERCKNGFYGNPVLGSGEHCQPCPCPGNPGSNHFNGHSCQSDYSANQIICNCNQGYTGSRCDQCAPGYYGNPEQPGGQCLPCQCNNNIDPNDPESCDPRTGRCLRCLYHTHGQFCSECEPGYHGNAIAQECRRCTCVTAGTLQSACHDGLCHCDPESGACPCRENVRGRVCDQCAPNHWNFGEERGCEPCDCD-PQNSLGTHCNMFTGQCHCRPGFGGKTCTECEQFYWGDPQV--ECRECNCHVHGSNLRVCDRTTGQCDCQEGKAGQKCDECARGFTGAFPNCVRCHQCFQLWDDIICQIKRDLDHIHFTAQRILESGVMPGVGDARIRELEEKLKQVKDLIGSGRNESIHQLIGQAIDDIRAEIAVTDGRLMAISQEINTTAGEERFLQRTLSIMEDELRDLNYTVANMQLYHLPVGFADQFEKL-----KKDYQKSLQAEEKCNASVSGPFSPVEQSNK--------TRRITEELLEATKDEFLKAVEANNQSLNELQQEALKLGETVQDLSQKVC--GGHSSVSVNGSCP---DSQCGGAGCRDDR------GDLICGGEGCNGTVGSARAALNQSVNTIQSLMAANEELQNISRKLQEIASLTQDVKNQTMKTLEKAQEKKKHFEDNNKNLKDFIKMIKDFLTGEGADPESIEKVALQVLSITLPFNRTTLDKITTEIRDSLSNLTNVEAILNQTADTVNKAKELLSKAKDAKAQAEGVKDAANSTKQALEASEKAIEKATTALKEAQSNLNNTQNATAEVESQLLLLERKQMDIVMRLQNLSAEAEALRNKTEMNRRMADDALALANNATLRASSLEQSLNDTEEQYQELLRKVDSLGGESGSLDTISQKVRMMKSDAEDLFNKAKDGIKELEELTNTFKDNEDRMQKQLVELEDLKQNATDVRDELRRKMQTFSSC 1838 HSP 3 Score: 112.079 bits (279), Expect = 4.694e-24 Identity = 92/330 (27.88%), Postives = 134/330 (40.61%), Query Frame = 3
Query: 858 RCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFD------------------------------PVVYQQSRKMSG-----------------------GVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTK----FPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDE 1676
RC C GHA C + G+ + DC+ N G CE+CK+ + +P L S C+ C C + S HF+ P Y + G G C C ++T + C C+ GY+ N A+A Q C +C C + GT +C +PE +G C C+ENV G CD+C W F EE GC C+C +L C+ TG+C+C+ G C+ C+ Y+ C C C +GS + + CD+ +G+C C+ G AG +CDE
Sbjct: 913 RCQCNGHADLCDPQTGECR---------DCRDNAAGHQCERCKNGFYGNPVL----GSGEHCQPCPCPGNPGSNHFNGHSCQSDYSANQIICNCNQGYTGSRCDQCAPGYYGNPEQPGGQCLPCQCNNNIDPNDPESCDPRTGRCLRCLYHTHGQFCSECEPGYHGN-AIA----QECRRCTCVTAGTLQSACHDGLCHCDPE------SGACPCRENVRGRVCDQCAPNHWNF--GEERGCEPCDCDPQNSLGTH-CNMFTGQCHCRPGFGGKTCTECEQFYWGDPQVE---CRECNCHVHGS-NLRVCDRTTGQCDCQEGKAGQKCDE 1211 HSP 4 Score: 107.071 bits (266), Expect = 1.453e-22 Identity = 80/252 (31.75%), Postives = 117/252 (46.43%), Query Frame = 3
Query: 2673 CMCKKGYSGQFCDQCETNH-----FGNAISKDGTCQPCRCSGNIDQRIED------SCNSKTGECRKCLFNTSGFNCEKCVSGYF--GNGAVR---TCTKCVCNHMGTNSKWQGICNHET--------GKCPCLPNVVGLQCDRCQDKFFNLSS--QIGCLPCNCDYPIGA--LNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKG 3344
C+CK G C++C+ H TC+ C C+G+ +Q D + N G C CL NT G NCE C Y+ N VR C C C+ +G+ G+C+ T G+C C NV G++CD C+ ++ LS GC PCNCD P G + C+Q++G C C+ +G+ C+ C +WG GC C C+ G++ +C +G C C+ + GR C G
Sbjct: 297 CVCKHNTEGLNCERCKDFHKDLPWRPAEAENPHTCKECNCNGHSNQCHFDMAVYLATGNVSGGVCDNCLHNTVGRNCEMCQPFYYQDPNRDVRDPQVCLLCDCDPVGSFDG--GVCDSHTDLDMGMIAGQCRCKANVKGIRCDDCKVGYYGLSQNDPQGCQPCNCD-PRGIIRMGDPCDQISGDCSCKRYVTGRYCDQCLPEYWGLSNDQFGCRPCDCDFGGALNNRCMMESGQCDCRRHLIGRQCSEVQSG 545 HSP 5 Score: 97.4413 bits (241), Expect = 1.079e-19 Identity = 72/257 (28.02%), Postives = 110/257 (42.80%), Query Frame = 3
Query: 2364 GKCLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTC-----------DSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKNN--SIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFG-NGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFF 3050
G+C+C+ EG +C+ CK + + P+ C C CN S C + G C NC N+ G NC+ C+ YY D +R + +C C C + S L A C CK G CD C+ ++G + + CQPC C R+ D C+ +G+C C +G C++C+ Y+G + C C C+ G C E+G+C C +++G QC Q +F
Sbjct: 295 GRCVCKHNTEGLNCERCKDFHKDLPWRPAEAENPHTCKECNCNGHSNQCHFDMAVYLATGNVSGGVCDNCLHNTVGRNCEMCQPFYYQDPNRDVRDPQVCLLCDCDPVGSFDGGVCDSHTDLDMGMIAGQCRCKANVKGIRCDDCKVGYYGLSQNDPQGCQPCNCDPRGIIRMGDPCDQISGDC-SCKRYVTGRYCDQCLPEYWGLSNDQFGCRPCDCDFGGA---LNNRCMMESGQCDCRRHLIGRQCSEVQSGYF 547 HSP 6 Score: 86.2705 bits (212), Expect = 3.351e-16 Identity = 74/260 (28.46%), Postives = 102/260 (39.23%), Query Frame = 3
Query: 927 VYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHC--DSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNC-VMMGTLENRGCDSSTGKCY-CKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNK------TCDKYSGKCLCKNGFAGIRCDE 1676
+ G C C+ G+ C C+ P CR+C+CN H+ C DP G C +C+ N CERCK+G+Y N + S + C C C + F Q D N C C + G++CD+C G++ CL C C + + CD TG+C C G CS C+ GY NA C C C G+ + CD SG C C+ G CD+
Sbjct: 883 LTGQCPCRQGAIGRQCSDCQPGQWGFP----------SCRRCQCNGHADLC--DPQT---------GECRDCRDNAAGHQCERCKNGFYGN--PVLGSGEHCQPCPCPGNPGSNHFNGHSCQS---DYSANQIICNCNQGYTGSRCDQCAPGYYGNPEQPGGQCLPCQCNNNIDPNDPESCDPRTGRCLRCLYHTHGQFCSECEPGYH--GNAIAQECRRCTCVTAGTLQSACHDGLCHCDPESGACPCRENVRGRVCDQ 1114 HSP 7 Score: 71.2478 bits (173), Expect = 9.914e-12 Identity = 42/134 (31.34%), Postives = 73/134 (54.48%), Query Frame = 3
Query: 2061 VLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCK---CNVTG--SRNNNCNKIGGQCQCLPNVSGKTCERCASESYGF--DARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPF 2441
+ G+C AN V +CD + ++ ++++DP C+ C+ G + C++I G C C V+G+ C++C E +G D GC+ C CD G+LN++C ++G+C CR + G+ C + GY+ P
Sbjct: 421 IAGQCRCKAN-VKGIRCD--DCKVGYYGLSQNDPQGCQPCNCDPRGIIRMGDPCDQISGDCSCKRYVTGRYCDQCLPEYWGLSNDQFGCRPCDCDFGGALNNRCMMESGQCDCRRHLIGRQCSEVQSGYFCAPL 551 HSP 8 Score: 71.2478 bits (173), Expect = 1.088e-11 Identity = 50/155 (32.26%), Postives = 74/155 (47.74%), Query Frame = 3
Query: 1233 LKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCL-CKNGFAGIRCDEIKSEWY 1694
L C CD G+ S C Q G C CK NV G +CD+C G + F +GC CNC G+ ++ CD TG+C C+ G CS C+ G + + C C+C+ + CD +G+C C++ AG +C+ K+ +Y
Sbjct: 818 LACQCDPQGS-LSSECNQV--------GGQCRCKPNVMGRRCDQCAPGTYGFG---VSGCTACNCHPDGSSSHQ-CDPLTGQCPCRQGAIGRQCSDCQPGQWGFPS-----CRRCQCNGHAD----LCDPQTGECRDCRDNAAGHQCERCKNGFY 950
BLAST of Netrin-4 vs. Planmine SMEST
Match: SMESG000062589.1 (SMESG000062589.1) HSP 1 Score: 3346.21 bits (8675), Expect = 0.000e+0 Identity = 1673/1689 (99.05%), Postives = 1680/1689 (99.47%), Query Frame = 3
Query: 63 MTLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLTVFTIESNNKX-XXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYLNGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLSRLLVLQDKIYSKSEFHAQCLP 5126
MTLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYG+TWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCK+FYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLTVFTIESNNKI PPSIIDIVVIPLLDVNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCL+NTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLE+NMKSLKEIKKYFPI YDIIISNFSEKLSRLKYYVYN NIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNI LIKDKLFYRNEELRNINLKFI QKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVT GAIQSRR+ESVNLLIKIKTYLNGSADLKDFESLIENINKFEINFKENEIIALDKQI SRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNIST NRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTFQQIKIKQEFQKAKLRNLKIDTKVVVNKSNYFKLKSASN+KISERFQSLLNKSHNLNDKIKASHYKLKLNKKLI+DKELKLQ LSKDIDEKLSRLLVLQDKIYSKSEFHAQCLP
Sbjct: 1 MTLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGKTWNKYAYFSDNCPRDFPSIPERKRSNINEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKEFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFDSFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIPNKCLEKDLPIVLTVFTIESNNKIPPPSIIDIVVIPLLDVNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLYNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEKNMKSLKEIKKYFPIAYDIIISNFSEKLSRLKYYVYN-NIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNIILIKDKLFYRNEELRNINLKFISQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTSGAIQSRRLESVNLLIKIKTYLNGSADLKDFESLIENINKFEINFKENEIIALDKQIISRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTFNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTFQQIKIKQEFQKAKLRNLKIDTKVVVNKSNYFKLKSASNAKISERFQSLLNKSHNLNDKIKASHYKLKLNKKLILDKELKLQFLSKDIDEKLSRLLVLQDKIYSKSEFHAQCLP 1688
BLAST of Netrin-4 vs. Planmine SMEST
Match: SMESG000019909.1 (SMESG000019909.1) HSP 1 Score: 382.489 bits (981), Expect = 2.397e-108 Identity = 434/1688 (25.71%), Postives = 716/1688 (42.42%), Query Frame = 3
Query: 123 DNVCRKTICNPRAGDLL-IGRESFLRASSTCGLTNSERYCVVTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSRLPQDPFRWWQSMSGVHN--------VSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDFPSIPERKRSNI---NEVLCTSEFSSIIPSEGGTVLFSADTSDWVNNDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNKYYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGT--LENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCK--NGFAGIRCDEIKSEWYIEKPTYPLEHLKEIKINLPPVSKSGYYNFVITTDTKNMQFFD---------------------SFIIVLSIYHVGKSSCQSYQDTMQLIKDSRYIVIP-----NKCLEKDLPIVLTVFTIESNNKXXXXXXXXXXXXXXXXNSTKTRKVLGECLNYANLVSSKKCDFLNAEISTNTVTKSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARG-----CKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP------FCHSCKCNQMSTTCD--SKTGK----CLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISK-DGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFP----KEIDDVCTSL------EQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIIT--------IENSTDYAKAMI------SKNKIRDVTGAFNELKKSLKNILNGASE--SKKIYQNITLIKDKLFYRNEELRNINLKF------IRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPD-KCYRHAPMNKYCM-----GKSNQFFNLYASLLLDRTNMSIILKEFN------QQFNISIN-SKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYLNGSADLKDF-----ESLI--ENINKFEINFKENEIIALDKQIRSRIRKLNNVNNILD---------KSKFNRKQCDVFKKSAVRAKEDTSY------LLNSIKNISTLNRMTQKF-EIIMKQF---VMNFANALFSLKGNLFHMFKNENEAFQK-----QNNISYVTERIKFLKL---YIMKIHK 4769
D+ + IC P + GR+ A++TCG+ ++YC+ +S R S+C CD N +R S Q+P WWQS + N +++ I +F TF HL+FK+ RP+AM + + D W + YF+ +C +F +IP K + +EVLCT +FSS+ G V+F+ +++ + N+K D + IR++ ++HTFG+ +D + Y+YAI I GRC C GH ++C+ G+ ++ +Y VC H+T GQ CEKC +FY D PW A ++ N C++C CN +S SC F+ +Y +S +SGG+C NC+ NT+ +CE C G+Y+N + C+ C+CD +G P Q G C C+ +EG++C +CK ++ F N GC CNC + G E C+ STG CYCK V G C C G+F L+ ++ GC C C + K C G L + + F G D K PT+ + +I + P ++ F+ D ++ FF SF I I + S S + +QLI D+ + +P N D VL F + + + +D + N + L Y L +++ T S +K N++ + K C C P +G +CE CA + ++G C KC C+ + + C TG C C+ G +CD C GY+ P C C C T C+ +GK C +C N GY C C + ++G+ I CK C+C G ++ C I T C +G C+ C+ +F + + C+ C C+ + C+ TG C C G C+ C GYF C C C +G+ + C+ TG+C C P V GL+CDRC+ + FNLSS GC C CD P G+ N C+ TG C C+ G CN C + T+ GC KC + + R + N +D N + + + + F + ++ F +K KE++ T+ E+ SL +I+ ++ +F L LK Y+ + ++ N II+ E ST+ K++I S+ + + +F +L +N +N +S+ K++ I+D+ F E+ +N F I SK +N+I E+++ +++F NT+++ + +F V PD K NK M + + L + N++ +K N + FN +N SK + ++K NTT++ I + + +++N LI ++ KD+ ES+I +NI KF N+++ L K+ + + L+ + + D + K N + + ++ K D S L++ I I+++ T+ E +K F + N +N + + N F++ E + QN + + I L+L ++++I+K
Sbjct: 37 DSSGQPQICYPPFQNFASAGRKVI--ATNTCGIRGRQKYCIGLSSAEMR-SRCAYCDKNNVNERHSPDF---------LTDQNPNNWWQSETWADNRELFNRESINLTIDLGKQFHVTFLHLQFKSPRPSAMVLYKKYDNNSPWQPWQYFASDC-LNFFNIPYVKGYIVKRHDEVLCTEDFSSLNEFYNGIVMFATIYGRPGSDNFFANQKLMDWATASQIRIELRKMHTFGEQ--NDEEELLMTYFYAIKNLQIAGRCWCNGHGNECRKSTGE-GLEPQLYCVCHPSHHTVGQSCEKCDNFYQDRPWKAASTSNANTCQRCNCNGNSESCIFNDELYIKSGNISGGLCLNCKGNTKGPHCEECTEGFYKN----PDQPNRCIPCNCDPIGRVSPQCNSQ----------GVCTCQPGIEGDKCTRCKRDYFNFGSN---GCQPCNCHLPGVNITERLHCNPSTGTCYCKKNVQGEKCDGCVAGFFGLTESDPLGCRPCFCFAH----TKDCTMAKGFLLTRIESDFIGT-VDSWKYGIGSLAPTF--NPMLDITTSPPSITW-----FIGRDDWRDDYFFSAPKKFLGNRRLSYNLNLKLTLSFGIRPDISTLN-SKWMSMINDVQLISDTLAVSVPFDIGSNTVPNVDKNTVLYTFRLNEESGLWRDKMDYFRFNSILSNLEAIKIRLTNPSGYVRL----------HKVALETALDSQLVK-SLNLSLPAAESVEK----CLCPPQYTGLSCENCAPGYHREYSKGSEFIKCIKCSCN---NQSDTCDVQTGVCHCKYNSIGDNCDKCAIGYYGDPTSNLRNVCKPCGC-PGGTACELIRISGKDRIVCTDCPNNLGGYRCDRCRDNFFGNPKLNITCKPCECNGNVDYFSS---CDTI---TGKCPKCIYNSAGDNCETCKPGYFLLSNERAPNRCKRCICNPHGSLTGGVLCDINTGNCF-CKKLVIGQKCDSCKPGYFNLTVNEQCQPCNCLPIGS---IKENCHQTTGQCKCKPGVTGLRCDRCEFEHFNLSS-TGCQKCMCD-PYGSSNLQCHWKTGMCSCKTNVLGNKCNQCMENHHNLTTE--GCQKCPPCYGLIQKRISTLKADIARLSDSVYSVNM------SDSTFGNMTESLNIARNHNETFAKLFQNFIQNYDKIEKFAIELKIVENSLKELETNLTNFKNDKMNERVQASLNDIQNMENAIKGLLNKDFQRYLDLLKDYIKSK------SENNQIISKVAEEAKEYEISTNQQKSIINNIYTESEKHLANSLTSFVDLIGRYRNYVNESSQFDIKELSALSKKIQDESFKNLMEISVLNKSFEKFENSINNASKIHENIIKEINEVILSFLDAE-NTVSQ----LDWKFRQLVINLPDLKLLEEQIKNKTYMISLKTRNVTEAQEIMMKLKEAQNNLTDAIKMANKTLFKLEDFNEVVNKSKFEAEEHIKILPYLNTTLQEIILEIQHLNDSVSDTINSLISVQ---------KDYQVIYNESMIKDKNIKKF-----NNQVLQLLKENKENMNVLDKIKTLSDEKLNHHLIVQDKLNELMRSLNETKSLSEKHDDSVTGLNASLVDIINRINSIQNETESMDERTLKFFSERIDNLSNVIENYYQNEFNIISKSMEDMDREWQELQNESEFTNKEINRLQLIDDHMVQINK 1614 HSP 2 Score: 222.246 bits (565), Expect = 6.961e-58 Identity = 241/921 (26.17%), Postives = 399/921 (43.32%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYW-------NFPFCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI--LCKKCQCPGLSSLKNNSIGCSLI--FNTTAFTCM-CKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYF---GNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDG-KMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSS-GVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYNSNIIDFNTQQNSIITIENSTDYAKAMISKNKIRDVTGAFNELKKSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNINLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTITESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLIDVTRGAIQSRRIESVNLLIKIKTYLNGSADLKDFESLIENINKFEINF--KENEIIALDKQIRSRIRKLNNVNNILDKSKFNRKQCDVFKKSAVRAKEDTSYLLNSIKNISTLNRMTQKFEIIMKQFVMNFANALFSLKGNLFHMFKNENEAFQKQNNISYVTERIKFLKLYIMKIHKVFSNTXXXXXXXXXXXXXXLRNLKIDTKVVVNKSNYFKLKSASNSKISERFQSLLNKSH---NLNDKIKASHYKLKLNKKLIVDKELKLQSLSKDIDEKLS 5063
KCLC G C+ C PGY F C C CN S TCD +TG C +CK NS G NC C GYYGD + + +CK C CPG C LI C C G CD+C N FGN + TC+PC C+GN+D SC++ TG+C KC++N++G NCE C GYF A C +C+CN G+ + +C+ TG C C V+G +CD C+ +FNL+ C PCNC PIG++ +C+Q TGQC C+ G +G C+ C+ F GC KC C+ GS QC+ G+C CK + G C+ CM+ C C C+ + +K +L +S V MS+ F NM I + + + NF + +++ + I+ +NS+ +E + K +++ ++ ++K +LN K + + L+KD + ++E N + + +++K+ + N+ K++I N TES + + V +G+ + N S D +S + SK+I+D K L+++ ++ ++ E K + +N ++ K E++I+ IN+ ++F EN + LD + R + L ++ + ++ K + ++ A+E L + N++ +M K LF L+ + K++ EA + + Y+ ++ + L I ++ S+T L +++ D +V+ N+S +K + K + + LL ++ N+ DKIK + KLN LIV KL L + ++E S
Sbjct: 718 KCLCPPQYTGLSCENCAPGYHREYSKGSEFIKCIKCSCNNQSDTCDVQTGVC-HCKYNSIGDNCDKCAIGYYGDPTSNLRNVCKPCGCPG-------GTACELIRISGKDRIVCTDCPNNLGGYRCDRCRDNFFGNP-KLNITCKPCECNGNVDYF--SSCDTITGKCPKCIYNSAGDNCETCKPGYFLLSNERAPNRCKRCICNPHGSLTGGV-LCDINTGNCFCKKLVIGQKCDSCKPGYFNLTVNEQCQPCNC-LPIGSIKENCHQTTGQCKCKPGVTGLRCDRCE--FEHFNLSSTGCQKCMCDPYGSSNLQCHWKTGMCSCKTNVLGNKCNQCMENHHNLTTEGCQKCPPCYGLIQKRISTLKADIARLSDSVYSVNMSDSTF------------GNMTESLNIARNHNETFAKLFQNFIQNYDKIEKFA-----IELKIVENSLKELETNLTNFKNDKMNERVQASLNDIQNMENAIKGLLN-----KDFQRYLDLLKDYIKSKSE--NNQIISKVAEEAKEYEISTNQ-QKSIIN------NIYTESEKHLANSLTSFVD---------------LIGRYRNYVN--ESSQFDIKELSAL-------------SKKIQDESFK---------NLMEI---SVLNKSFE------KFENSINNAS--KIHENIIKEINEVILSFLDAENTVSQLDWKFRQLVINLPDLKLLEEQIKNKTYMISLKTRNVTEAQEIMMKLKEAQNNLTDAIKMANK--------------TLFKLEDFNEVVNKSKFEAEEHIKILPYLNTTLQEIILEIQHLNDSVSDT-----------INSLISVQKDYQVIYNES---MIKDKNIKKFNNQVLQLLKENKENMNVLDKIKTLSDE-KLNHHLIVQD--KLNELMRSLNETKS 1511
BLAST of Netrin-4 vs. Planmine SMEST
Match: SMESG000050895.1 (SMESG000050895.1) HSP 1 Score: 177.563 bits (449), Expect = 1.276e-45 Identity = 129/432 (29.86%), Postives = 210/432 (48.61%), Query Frame = 3
Query: 198 ASSTCGLTNSERYCV----VTTSDSGRKSKCTICDSKNPYQRFSSTHSHRIENVVSR-LPQDPFRWW----QSMSGVHNVSIEISFESEFQFTFTHLRFKTYRPAAMYIERSKDYGRTWNKYAYFSDNCPRDF--PSIPERKR----SNINEVLCTS-----------------EFSSIIPSEGGTVLFSADTSDWVN-NDVYWNKKRNDITAITNIRVQFVRLHTFGDWKVDDNPNIRNK-----YYYAISEWIIRGRCSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGF 1379
+STCG E+ C TT+++ + C+ICD+K+ S+ ++ R +P++ W+ Q G + V+I +S + F+ + L+ P ++ I +S D+G +W + +FS +C R F P+ E+ +NI+EVLC FS+I G +S D +W+ D+ + R T R++ + HT + PN+ N ++ +S+ + GRC C GHA++C + ++K CDC HNT+GQ CE+CK Y D PW A + + N C+ C+C +HS C F +Y+Q+R GGVC C+HNTE C+ C + +YR+I + Q C C C ++G+ I++ E D G C C++ V G CD+CK G+
Sbjct: 54 TTSTCGTNGPEQICRNWYDKTTAEA--RQYCSICDAKH--------RSYPPSHITDRHIPKNQTCWFSGPLQENPGQNEVNITVSLKKSFEVYYIALQICGTLPDSIAIYKSLDFGVSWKPWQFFSQDCYRAFKMPTTNEQNSQITPANIHEVLCVELKAPERYNDYGQAETVLPFSTIYGRPSGPP-WSQDLVEWMTMTDLRISLMRFTPEKSTPYRLKELYYHT--ESLPQAPPNLVNHKTEPIVHFGLSDLAVGGRCKCNGHANRCLIDDND-EIK------CDCHHNTEGQECERCKSNYLDRPWQRATRQNANVCKLCQCYNHSDKCMFSMGMYKQTRGQHGGVCLECKHNTEGHACDECAAFHYRDITKPVTHPQACTSCKCHTIGS------IKQNECD--KKTGQCYCRDGVTGQTCDRCKDGY 457 HSP 2 Score: 60.8474 bits (146), Expect = 7.737e-9 Identity = 52/188 (27.66%), Postives = 77/188 (40.96%), Query Frame = 3
Query: 2355 NDTGKCLCREGVEGQHCDTCKPGYWNFPF----------CHSCKCNQMSTTC-----------DSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGI----LCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKC 2843
ND KC C EGQ C+ CK Y + P+ C C+C S C G CL CK N+ G+ C C +Y D ++ + C C+C + S+K N + C C+ G +GQ CD+C+ + + T +PC S + D N+ + C+KC
Sbjct: 314 NDEIKCDCHHNTEGQECERCKSNYLDRPWQRATRQNANVCKLCQCYNHSDKCMFSMGMYKQTRGQHGGVCLECKHNTEGHACDECAAFHYRDITKPVTHPQACTSCKCHTIGSIKQNEC------DKKTGQCYCRDGVTGQTCDRCKDGY----KQSNSTVRPCIPSSG----MADMANT-SPVCKKC 486 HSP 3 Score: 55.4546 bits (132), Expect = 3.722e-7 Identity = 55/181 (30.39%), Postives = 77/181 (42.54%), Query Frame = 3
Query: 2727 HFGNAISKDGTCQPCRCSGN-----IDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVR-------TCTKCVC-NH----MGTNSKWQGICNHETGKC-PCLPNVVGLQCDRC-----QDKFFNLSSQIGCLPCNCDYPIGALNH-SCNQLTGQCYCQAGRSGKMCNNCKDGF 3197
HFG +S C+C+G+ ID E C+ C NT G CE+C S Y R C C C NH M + ++ G C C N G CD C +D ++ C C C + IG++ C++ TGQCYC+ G +G+ C+ CKDG+
Sbjct: 288 HFG--LSDLAVGGRCKCNGHANRCLIDDNDEIKCD--------CHHNTEGQECERCKSNYLDRPWQRATRQNANVCKLCQCYNHSDKCMFSMGMYKQTRGQHGGVCLECKHNTEGHACDECAAFHYRDITKPVTHPQACTSCKC-HTIGSIKQNECDKKTGQCYCRDGVTGQTCDRCKDGY 457 HSP 4 Score: 55.0694 bits (131), Expect = 5.648e-7 Identity = 32/78 (41.03%), Postives = 41/78 (52.56%), Query Frame = 3
Query: 2217 GGQC-QCLPNVSGKTCERCASESYG------FDARGCKKCKCDLTGSL-NSQCYNDTGKCLCREGVEGQHCDTCKPGY 2426
GG C +C N G C+ CA+ Y + C CKC GS+ ++C TG+C CR+GV GQ CD CK GY
Sbjct: 380 GGVCLECKHNTEGHACDECAAFHYRDITKPVTHPQACTSCKCHTIGSIKQNECDKKTGQCYCRDGVTGQTCDRCKDGY 457 HSP 5 Score: 54.6842 bits (130), Expect = 6.927e-7 Identity = 46/172 (26.74%), Postives = 72/172 (41.86%), Query Frame = 3
Query: 2889 YFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIG-------CLPCNC-------DYPIGALNHSCNQLTGQCY-CQAGRSGKMCNNCKDGFWGDPTKL----NGCTKCYCNLDGSVFK-QCNRNNGVCRCKPGITGRNCDMCMKG 3344
+FG + +C CN I +++ KC C N G +C+RC+ + + Q C C C + +G + Q G C C+ G C+ C + D TK CT C C+ GS+ + +C++ G C C+ G+TG+ CD C G
Sbjct: 288 HFGLSDLAVGGRCKCNGHANRCL---IDDNDEIKCDCHHNTEGQECERCKSNYLDRPWQRATRQNANVCKLCQCYNHSDKCMFSMGMYKQTRGQHGGVCLECKHNTEGHACDECAAFHYRDITKPVTHPQACTSCKCHTIGSIKQNECDKKTGQCYCRDGVTGQTCDRCKDG 456
BLAST of Netrin-4 vs. Planmine SMEST
Match: SMESG000056013.1 (SMESG000056013.1) HSP 1 Score: 180.644 bits (457), Expect = 5.275e-45 Identity = 150/458 (32.75%), Postives = 205/458 (44.76%), Query Frame = 3
Query: 2193 RNNNCNKIGGQCQ-CLPNVSGKTCERCASESYGFDARG-----CKKCKC--------DLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP----FCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRI--EDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWG-------------------------------------------DPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSC 3377
+N C+ + G+CQ C N +G CE CAS YG DA+ CKKC C +L S +S+ Y KCL G HCD C GY+ P C CKCN +CD TG+C C N+ G CQ C++G+YGD CKKC C L S N + C C+ + + CD C + + KD C PC C I RI E CN+ +G+C CL + +G CEKCV GYF C KC+C+ ++ C+ GKC C NV+ CD+C++ + +++ C+PCNC G+LN C+ TG C C + + C+ C G++G D + GC KC C ++GS CN+ +G+C CK G CD C +G G PNC C
Sbjct: 397 HSNTCDSLNGECQNCTHNTAGAHCEDCASGYYG-DAKSKNLNFCKKCPCPRLDYQMTELCSSYSSENY-VCHKCL--HNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS-CKKCDCDSLGSTSENC-------DKLTGECDCRTNFVNRQCDSCTSGLY----LKDKKCIPCNCE--IQGRISSEKFCNTTSGQCF-CLESVNGDRCEKCVEGYFN---YPNCKKCLCSPHALSAD----CDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR--GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYG-FPNCQPC 823 HSP 2 Score: 176.407 bits (446), Expect = 9.056e-44 Identity = 118/334 (35.33%), Postives = 174/334 (52.10%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYW---NFPF---CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCM-CKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHS--CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMC 3335
KC C G G C++C+ GY N + C +C C S TCDS G+C NC N+ G +C++C +GYYGD L CKKC CP L CS +++ + C C SG CD+C ++GN K +C+ C+C+ N ++SC+ TGECR C +NT G C+ C G++G+ ++C KC C+ +G+ S+ C+ TG+C C N V QCD C + + C+PCNC+ G ++ CN +GQC+C +G C C +G++ P C KC C+ ++ C+ NNG C+C + R CD C
Sbjct: 358 KCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPRLDYQMTEL--CS-SYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP-KKKISCKRCKCNNN-----QESCDPVTGECR-CSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSEN---CDKLTGECDCRTNFVNRQCDSCTSGLYLKDKK--CIPCNCEIQ-GRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYP----NCKKCLCS-PHALSADCDLNNGKCKCGSNVINRICDKC 670 HSP 3 Score: 166.392 bits (420), Expect = 9.601e-41 Identity = 134/406 (33.00%), Postives = 199/406 (49.01%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDA--RGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPG-YWNFPFCHSCKCN---QMSTT--CDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCS-GNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGS---VFKQCNRNNGVCRCKPGITGRNCDMCMKGT 3347
+CKCN + +C+ + G+C+C N G C+ C YG DA + CKKC CD GS + C TG+C CR + CD+C G Y C C C ++S+ C++ +G+C C + G C+ C GY+ + CKKC C S S C L C C + CD+CE H+ N + C PC C G+++ + C+ +TG C KC N + +C KC GYFG V C KC + G +CN+E G C C PN +G C++C + ++ + IGC CNC Y G+ ++ CN+++G C C+ G C+ C +G++G P C C C +G+ +C++ G C+CK + G C +C KGT
Sbjct: 496 RCKCN---NNQESCDPVTGECRCSYNTMGTECQLCKHGFYG-DALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLESVNGDRCEKCVEGYFNYPN----CKKCLC----SPHALSADCDL----NNGKCKCGSNVINRICDKCENLHYWNNRTD---CVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFG-LDVTGCKKCPPCYNGQ------VCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNC-YINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGT 863 HSP 4 Score: 146.747 bits (369), Expect = 8.963e-35 Identity = 117/366 (31.97%), Postives = 168/366 (45.90%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQ--CYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKC--NQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNH--SCNQLTGQCYCQAGRSGKMCNNCKDG-FWGDPTKLNGCTKCYC 3242
KC C+ GS + NC+K+ G+C C N + C+ C S Y D + C C C++ G ++S+ C +G+C C E V G C+ C GY+N+P C C C + +S CD GKC C N C CEN +Y + C C C G SL + C + C C + + + C +C+ +FG ++ C PC +G + CN++ G C C NT G C KC + C KC C N + ICN +G C C G +CDRC + ++ + C PC+C N C+++TGQC C+ G C C+ G F D T GC C+C
Sbjct: 542 KCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKK-CIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWNNRTD--CVPCNC-GRGSL---NPKCDM----ETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY-NGQV-------CNNE-GICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCY---INGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN---CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 879 HSP 5 Score: 98.9821 bits (245), Expect = 2.887e-20 Identity = 88/322 (27.33%), Postives = 130/322 (40.37%), Query Frame = 3
Query: 861 CSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKD-FYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAIN------------------------------------------SSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLEN-RGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
C+CY H++ C + G+ Q +C HNT G HCE C +Y D A + N C+KC C + S VC C HNT +C+RC GYY N I+ +++C KC CDSLG+ + C + G C C+ N QCD C G + ++ C+ CNC + G + + + C++++G+C+C V G+ C C GYFN N C C CS + + CD +GKC C + CD+ ++ Y
Sbjct: 392 CACYNHSNTCDSLNGECQ---------NCTHNTAGAHCEDCASGYYGD-----AKSKNLNFCKKCPC----PRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSEN-CDKL--------TGECDCRTNFVNRQCDSCTSGLYL----KDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPN-----CKKCLCSPHALSAD--CDLNNGKCKCGSNVINRICDKCENLHY 675 HSP 6 Score: 97.0561 bits (240), Expect = 1.358e-19 Identity = 139/593 (23.44%), Postives = 242/593 (40.81%), Query Frame = 3
Query: 2820 KTGECRKCLFN--TSGFNCEKCVSGYFG----------NGAVRTCTKCVCNHMGTN-SKWQGICNHETGKCP-CLPNVVGLQCDRCQDKFF-NLSSQIGCLPCNCDYPIGALNHSCN------QLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSC-----GDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEIDDVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYN--SNIIDFNTQQNSIITIENST---------DYAKAMISKNKIRDVTGAFNELK---KSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNI---NLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTIT------ESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLI--DVTRGAIQSRRIESVNLLIKIKTYLNGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLN 4445
+ KC+ N T G +C+ +SGY+ +G++RT + + N N + + +C+ TG C C+ N G +C C++ F+ N + C PC C P N + N L +C C+ G SGK C NC+ GF+G+P K C C CN GS CN G+C+C PG+ G CD C + C SC D ++ D V N K S+ ++ + S++ + + L I K++ + + I NFS + ++K I T+ N I+ ST + + IS I++ T +K K + NI AS K + + + + + Y +++ NLK ++ K+ M+ + T N NT++ E ++ +E + K +R + + K Q + + D ++ ISIN +EI+D S + + LI D + ++ + +S NL+ K+ N + + + NI + +I+A+D I ++L+
Sbjct: 1095 RVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQSCYSGCTKDLLVRIDTLNETVINFKWKTLPST--------LDLRLNKINISIQMSSELLNSINKFYKLKNE--IKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKILEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTI--------MADEINTNNKEYLNTMSTKKKEHELYIILINERMKDL----QKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDL------------ISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITR--------DILAVDYAINQTKKELD 1642 HSP 7 Score: 91.2781 bits (225), Expect = 6.290e-18 Identity = 73/248 (29.44%), Postives = 106/248 (42.74%), Query Frame = 3
Query: 933 GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKT--CDKYSGKCLCKNGFAGIRC 1670
G C C N + C+KC++ + W N+ +C C C S + D M G C C N ++C +C GY+ + + + C C+ + N G C+C N GN C+KC W FN E GC KCNC + G+ N C+ +G C CK+ G+ C C GY+ N C C C+E G++ N+ CDK +G+C CK G +C
Sbjct: 654 GKCKCGSNVINRICDKCENLHY---W-----NNRTDCVPCNCGRGSLNPKCD---------METGNC-KCAQNVARRDCSKCDVGYF---GLDVTGCKKCPPCYNGQVCN----------------NEGICVCPPNTIGNLCNKCSEDSWDFN--ETIGCKKCNCYINGSYSNI-CNKISGICACKTAFFGSKCDRCNEGYYGFPN-----CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKC 856 HSP 8 Score: 90.8929 bits (224), Expect = 9.823e-18 Identity = 76/268 (28.36%), Postives = 113/268 (42.16%), Query Frame = 3
Query: 900 EGQVQVKEMVY-GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIE 1700
E + + EM + C+C G CE C+ Y + +C C C +HS++C D + G C NC HNT +CE C SGYY + + C KC C L + +C + + C N G CD+C G++ N ++ C +C C CD TG+C C G +C CK G++ +A C C C GS ++ CDK +G+C C+ F +CD S Y++
Sbjct: 345 EVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRV----NNVLYQGQCEACACYNHSNTC--DSL---------NGECQNCTHNTAGAHCEDCASGYYGD--AKSKNLNFCKKCPCPRLDYQMTELCSSYSSENYVCHK----CLHNTSGVHCDRCIEGYYG-NPKKKISCKRCKC----NNNQESCDPVTGECRCSYNTMGTECQLCKHGFY--GDALTKSCKKCDCDSLGST-SENCDKLTGECDCRTNFVNRQCDSCTSGLYLK 583 HSP 9 Score: 86.2705 bits (212), Expect = 2.081e-16 Identity = 66/207 (31.88%), Postives = 90/207 (43.48%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWNFPF--------------------CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKC 2927
KC+C G HC GY+ F C CN S CDS TG C NC GN G CQ C G+Y + C+ CQCP S+ N + C+ I ++ C C+ GYSG+ C+ C+ +GN I K C+ C C+ Q + +CN + G C+ C G C++C Y +TC C
Sbjct: 1101 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCP--SAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPI-KGIPCKSCDCNQQGSQSL--NCN-EFGICQ-CSPGVLGIKCDQCAPNYVVEK--KTCQSC 1298 HSP 10 Score: 85.5001 bits (210), Expect = 4.473e-16 Identity = 52/140 (37.14%), Postives = 69/140 (49.29%), Query Frame = 3
Query: 2220 GQCQCLPNVSGKTCERCASESYGF-DARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMS------TTCDSKTGKCLNCKGNSFGYNCQNCENGYY-GDTSRGILCKKCQCPGLS 2615
G C C PN G C +C+ +S+ F + GCKKC C + GS ++ C +G C C+ G CD C GY+ FP C C C + T CD TG+C CK N G C+ C G + D + C C C G+S
Sbjct: 745 GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQC-QCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVS 883 HSP 11 Score: 84.3445 bits (207), Expect = 8.620e-16 Identity = 68/217 (31.34%), Postives = 90/217 (41.47%), Query Frame = 3
Query: 2151 KSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCK-CN-----QMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSG 2783
++D + C C GS N C+ G C+C NV+ + C +C +G D GCKKC N Q N+ G C+C G C+ C W+F CK CN S C+ +G C CK FG C C GYYG + C+ C C + N C I C CK+ G C C F + + +G C C C G
Sbjct: 679 RTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC----PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICA-CKTAFFGSKCDRCNEGYYGFPN----CQPCSCAENGTKSNELTKCDKI----TGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFG 881 HSP 12 Score: 83.1889 bits (204), Expect = 2.138e-15 Identity = 96/340 (28.24%), Postives = 129/340 (37.94%), Query Frame = 3
Query: 927 VYGVCDCQHNTKGQHCEKCKD-FYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGT---------------------------------KFPS--ICIQEPEL---DLGLNAGHCICKENVEGNQCDKCK-IGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLC---------------------------KCSEYGSRDNKT-----------------CDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
V G C C +NT G C+ CK FY D+ T C+KC+C+S S+ + K++G CD C+ N ++ C+ C SG Y + C+ C+C+ G +P+ C+ P D LN G C C NV CDKC+ + +W N T C+ CNC G+L N CD TG C C V DCS C +GYF L ++ GC C KCSE N+T C+K SG C CK F G +CD +Y
Sbjct: 509 VTGECRCSYNTMGTECQLCKHGFYGDA--------LTKSCKKCDCDSLGSTS-------ENCDKLTGE-CD-CRTNFVNRQCDSCTSGLYL-------KDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYW----NNRTDCVPCNC-GRGSL-NPKCDMETGNCKCAQNVARRDCSKCDVGYFGL---DVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY 815 HSP 13 Score: 73.9442 bits (180), Expect = 1.192e-12 Identity = 53/139 (38.13%), Postives = 66/139 (47.48%), Query Frame = 3
Query: 1173 RCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLG-LNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGND-CSTCKIGYFNLSNANINGCSLCKC 1583
+C G+YR V C C C LGT C+ E D+G G CICK+ EG CD C IG+ ++ E CL C C + G++ C CK V +D C TCK GYFNL +AN GC C C
Sbjct: 28 KCIKGFYRPWNVLATELYPCTPCQCHGLGT--IGSCVSNGE-DIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGE---CLPCPCSIDGSVRT-SFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 159 HSP 14 Score: 58.5362 bits (140), Expect = 6.213e-8 Identity = 65/228 (28.51%), Postives = 88/228 (38.60%), Query Frame = 3
Query: 921 EMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDN-----CQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENR--GCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKC 1583
+M G C C N + C KC D + D C+KC P Y +G VC+N C NT C +C + N + C KC+C G+ + +IC + +G C CK G++CD+C G++ F C C+C GT N CD TG+C CK V G C C+ G F GC C C
Sbjct: 697 DMETGNCKCAQNVARRDCSKC-----DVGYFGLD---VTGCKKC------------PPCY------NGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGS-YSNICNKI--------SGICACKTAFFGSKCDRCNEGYYGF-----PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 879 HSP 15 Score: 55.8398 bits (133), Expect = 3.599e-7 Identity = 65/230 (28.26%), Postives = 89/230 (38.70%), Query Frame = 3
Query: 891 KAKEGQVQVKEMVYGV--CDCQHNTKGQHCE-KCKDFYNDSPWLPADKNSTN---------ECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQ-EPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETG--CLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGY 1535
K E + + V V C C T G HC+ +Y + LP S + +KC CN +S C D V G C NC N E C+ C+ G+Y+N + C C C S F C + E L+L C C+ G C+ C+ GF+ GN G C C+C G+ ++ C+ G C C V G C C Y
Sbjct: 1084 KVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC--DSVT---------GDCQNCMGNREGNKCQLCREGFYKNDK----NYDQCEPCQCPSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFY---GNPIKGIPCKSCDCNQQGS-QSLNCN-EFGICQCSPGVLGIKCDQCAPNY 1288 HSP 16 Score: 54.6842 bits (130), Expect = 9.622e-7 Identity = 34/81 (41.98%), Postives = 41/81 (50.62%), Query Frame = 3
Query: 2202 NCNKIGG----QCQCLPNVSGKTCERCASESYGFDARG--CKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGY 2426
NC +I +CQC SGK+CE C YG +G CK C C+ GS + C N+ G C C GV G CD C P Y
Sbjct: 1209 NCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNC-NEFGICQCSPGVLGIKCDQCAPNY 1288 HSP 17 Score: 53.1434 bits (126), Expect = 2.798e-6 Identity = 41/131 (31.30%), Postives = 63/131 (48.09%), Query Frame = 3
Query: 1329 CKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGC----DSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPT 1709
C N EGN+C C+ GF+K + N + C C C + C S KC C+ +G C C+ G++ I C C C++ GS+ + C+++ G C C G GI+CD+ + +EK T
Sbjct: 1168 CMGNREGNKCQLCREGFYKNDKNYDQ-CEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGI-PCKSCDCNQQGSQ-SLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKT 1294
BLAST of Netrin-4 vs. Planmine SMEST
Match: SMESG000056013.1 (SMESG000056013.1) HSP 1 Score: 180.259 bits (456), Expect = 6.219e-45 Identity = 150/458 (32.75%), Postives = 205/458 (44.76%), Query Frame = 3
Query: 2193 RNNNCNKIGGQCQ-CLPNVSGKTCERCASESYGFDARG-----CKKCKC--------DLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFP----FCHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRI--EDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWG-------------------------------------------DPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSC 3377
+N C+ + G+CQ C N +G CE CAS YG DA+ CKKC C +L S +S+ Y KCL G HCD C GY+ P C CKCN +CD TG+C C N+ G CQ C++G+YGD CKKC C L S N + C C+ + + CD C + + KD C PC C I RI E CN+ +G+C CL + +G CEKCV GYF C KC+C+ ++ C+ GKC C NV+ CD+C++ + +++ C+PCNC G+LN C+ TG C C + + C+ C G++G D + GC KC C ++GS CN+ +G+C CK G CD C +G G PNC C
Sbjct: 379 HSNTCDSLNGECQNCTHNTAGAHCEDCASGYYG-DAKSKNLNFCKKCPCPRLDYQMTELCSSYSSENY-VCHKCL--HNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGEC-RCSYNTMGTECQLCKHGFYGDALTKS-CKKCDCDSLGSTSENC-------DKLTGECDCRTNFVNRQCDSCTSGLY----LKDKKCIPCNCE--IQGRISSEKFCNTTSGQCF-CLESVNGDRCEKCVEGYFN---YPNCKKCLCSPHALSAD----CDLNNGKCKCGSNVINRICDKCENLHY-WNNRTDCVPCNCGR--GSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYG-FPNCQPC 805 HSP 2 Score: 176.407 bits (446), Expect = 9.897e-44 Identity = 118/334 (35.33%), Postives = 174/334 (52.10%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYW---NFPF---CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGIL--CKKCQCPGLSSLKNNSIGCSLIFNTTAFTCM-CKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHS--CNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMC 3335
KC C G G C++C+ GY N + C +C C S TCDS G+C NC N+ G +C++C +GYYGD L CKKC CP L CS +++ + C C SG CD+C ++GN K +C+ C+C+ N ++SC+ TGECR C +NT G C+ C G++G+ ++C KC C+ +G+ S+ C+ TG+C C N V QCD C + + C+PCNC+ G ++ CN +GQC+C +G C C +G++ P C KC C+ ++ C+ NNG C+C + R CD C
Sbjct: 340 KCNCPPGFNGLSCESCESGYRRVNNVLYQGQCEACACYNHSNTCDSLNGECQNCTHNTAGAHCEDCASGYYGDAKSKNLNFCKKCPCPRLDYQMTEL--CS-SYSSENYVCHKCLHNTSGVHCDRCIEGYYGNP-KKKISCKRCKCNNN-----QESCDPVTGECR-CSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSEN---CDKLTGECDCRTNFVNRQCDSCTSGLYLKDKK--CIPCNCEIQ-GRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYP----NCKKCLCS-PHALSADCDLNNGKCKCGSNVINRICDKC 652 HSP 3 Score: 166.392 bits (420), Expect = 1.171e-40 Identity = 134/406 (33.00%), Postives = 199/406 (49.01%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDA--RGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPG-YWNFPFCHSCKCN---QMSTT--CDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCS-GNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNHSCNQLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGS---VFKQCNRNNGVCRCKPGITGRNCDMCMKGT 3347
+CKCN + +C+ + G+C+C N G C+ C YG DA + CKKC CD GS + C TG+C CR + CD+C G Y C C C ++S+ C++ +G+C C + G C+ C GY+ + CKKC C S S C L C C + CD+CE H+ N + C PC C G+++ + C+ +TG C KC N + +C KC GYFG V C KC + G +CN+E G C C PN +G C++C + ++ + IGC CNC Y G+ ++ CN+++G C C+ G C+ C +G++G P C C C +G+ +C++ G C+CK + G C +C KGT
Sbjct: 478 RCKCN---NNQESCDPVTGECRCSYNTMGTECQLCKHGFYG-DALTKSCKKCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKKCIPCNCEIQGRISSEKFCNTTSGQCF-CLESVNGDRCEKCVEGYFNYPN----CKKCLC----SPHALSADCDL----NNGKCKCGSNVINRICDKCENLHYWNNRTD---CVPCNCGRGSLNPK----CDMETGNC-KCAQNVARRDCSKCDVGYFG-LDVTGCKKCPPCYNGQ------VCNNE-GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNC-YINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFP----NCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGT 845 HSP 4 Score: 146.747 bits (369), Expect = 9.640e-35 Identity = 117/366 (31.97%), Postives = 168/366 (45.90%), Query Frame = 3
Query: 2166 KCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQ--CYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKC--NQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKCVCNHMGTNSKWQGICNHETGKCPCLPNVVGLQCDRCQDKFFNLSSQIGCLPCNCDYPIGALNH--SCNQLTGQCYCQAGRSGKMCNNCKDG-FWGDPTKLNGCTKCYC 3242
KC C+ GS + NC+K+ G+C C N + C+ C S Y D + C C C++ G ++S+ C +G+C C E V G C+ C GY+N+P C C C + +S CD GKC C N C CEN +Y + C C C G SL + C + C C + + + C +C+ +FG ++ C PC +G + CN++ G C C NT G C KC + C KC C N + ICN +G C C G +CDRC + ++ + C PC+C N C+++TGQC C+ G C C+ G F D T GC C+C
Sbjct: 524 KCDCDSLGSTSENCDKLTGECDCRTNFVNRQCDSCTSGLYLKDKK-CIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKC-KCGSNVINRICDKCENLHYWNNRTD--CVPCNC-GRGSL---NPKCDM----ETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKCPPCY-NGQV-------CNNE-GICV-CPPNTIGNLCNKCSEDSWDFNETIGCKKCNCY---INGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPN---CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 861 HSP 5 Score: 99.3673 bits (246), Expect = 2.834e-20 Identity = 88/322 (27.33%), Postives = 130/322 (40.37%), Query Frame = 3
Query: 861 CSCYGHASKCKAKEGQVQVKEMVYGVCDCQHNTKGQHCEKCKD-FYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAIN------------------------------------------SSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLEN-RGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
C+CY H++ C + G+ Q +C HNT G HCE C +Y D A + N C+KC C + S VC C HNT +C+RC GYY N I+ +++C KC CDSLG+ + C + G C C+ N QCD C G + ++ C+ CNC + G + + + C++++G+C+C V G+ C C GYFN N C C CS + + CD +GKC C + CD+ ++ Y
Sbjct: 374 CACYNHSNTCDSLNGECQ---------NCTHNTAGAHCEDCASGYYGD-----AKSKNLNFCKKCPC----PRLDYQMTELCSSYSSENYVCHKCLHNTSGVHCDRCIEGYYGNPKKKISCKRCKCNNNQESCDPVTGECRCSYNTMGTECQLCKHGFYGDALTKSCKKCDCDSLGSTSEN-CDKL--------TGECDCRTNFVNRQCDSCTSGLYL----KDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPN-----CKKCLCSPHALSAD--CDLNNGKCKCGSNVINRICDKCENLHY 657 HSP 6 Score: 97.0561 bits (240), Expect = 1.299e-19 Identity = 139/592 (23.48%), Postives = 243/592 (41.05%), Query Frame = 3
Query: 2820 KTGECRKCLFN--TSGFNCEKCVSGYFG----------NGAVRTCTKCVCNHMGTN-SKWQGICNHETGKCP-CLPNVVGLQCDRCQDKFF-NLSSQIGCLPCNCDYPIGALNHSCN------QLTGQCYCQAGRSGKMCNNCKDGFWGDPTKLNGCTKCYCNLDGSVFKQCNRNNGVCRCKPGITGRNCDMCMKGTDGKMPNCSSCGDCWLQWDHFLIVVKNTFDKLHNSSGVFMSNVKFPKEID----DVCTSLEQNMKSLKEIKKYFPIGYDIIISNFSEKLSRLKYYVYN--SNIIDFNTQQNSIITIENST---------DYAKAMISKNKIRDVTGAFNELK---KSLKNILNGASESKKIYQNITLIKDKLFYRNEELRNI---NLKFIRQKSKDMDNLINEMSKNVITFNGVTCNTIT------ESNPLMCSEFCGGVGCKPDKCYRHAPMNKYCMGKSNQFFNLYASLLLDRTNMSIILKEFNQQFNISINSKEIKDAYVKPESEYNTTIKLI--DVTRGAIQSRRIESVNLLIKIKTYLNGSADLKDFESLIENINKFEINFKENEIIALDKQIRSRIRKLN 4445
+ KC+ N T G +C+ +SGY+ +G++RT + + N N + + +C+ TG C C+ N G +C C++ F+ N + C PC C P N + N L +C C+ G SGK C NC+ GF+G+P K C C CN GS CN G+C+C PG+ G CD C + C S C+ L+V +T N + + P +D + S++ + + L I K++ + + I NFS + ++K I T+ N I+ ST + + IS I++ T +K K + NI AS K + + + + + Y +++ NLK ++ K+ M+ + T N NT++ E ++ +E + K +R + + K Q + + D ++ ISIN +EI+D S + + LI D + ++ + +S NL+ K+ N + + + NI + +I+A+D I ++L+
Sbjct: 1077 RVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQC--PSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNCNE-FGICQCSPGVLGIKCDQCAPNYVVEKKTCQS---CYSGCTKDLLVRIDTL----NETVINFKWKTLPSTLDLRLNKINISIQMSSELLNSINKFYKLKNE--IKNFSIQFQKMKDDAKEKYGEIEKLKTRYNKILEDLRSTFTDINETISGHEQLGISNELIKNATAYLETIKNIYKEMPNITLAASVLKDVLKYVDELNQIIKYTQSHIQSDQINNLKILKNKTI--------MADEINTNNKEYLNTMSTKKKEHELYIILINERMKDL----QKIFRKMTKQRDNLRKKIQKLDAISKSFQDEKDL------------ISINVREIEDLMKNLSSNHLVSKSLIIPDTLKKHVEQQLNKSSNLIKKLTKDFNFLHNAEQAKEAYSNITR--------DILAVDYAINQTKKELD 1624 HSP 7 Score: 91.2781 bits (225), Expect = 6.495e-18 Identity = 73/248 (29.44%), Postives = 106/248 (42.74%), Query Frame = 3
Query: 933 GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKT--CDKYSGKCLCKNGFAGIRC 1670
G C C N + C+KC++ + W N+ +C C C S + D M G C C N ++C +C GY+ + + + C C+ + N G C+C N GN C+KC W FN E GC KCNC + G+ N C+ +G C CK+ G+ C C GY+ N C C C+E G++ N+ CDK +G+C CK G +C
Sbjct: 636 GKCKCGSNVINRICDKCENLHY---W-----NNRTDCVPCNCGRGSLNPKCD---------METGNC-KCAQNVARRDCSKCDVGYF---GLDVTGCKKCPPCYNGQVCN----------------NEGICVCPPNTIGNLCNKCSEDSWDFN--ETIGCKKCNCYINGSYSNI-CNKISGICACKTAFFGSKCDRCNEGYYGFPN-----CQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKC 838 HSP 8 Score: 90.8929 bits (224), Expect = 9.483e-18 Identity = 76/268 (28.36%), Postives = 113/268 (42.16%), Query Frame = 3
Query: 900 EGQVQVKEMVY-GVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIE 1700
E + + EM + C+C G CE C+ Y + +C C C +HS++C D + G C NC HNT +CE C SGYY + + C KC C L + +C + + C N G CD+C G++ N ++ C +C C CD TG+C C G +C CK G++ +A C C C GS ++ CDK +G+C C+ F +CD S Y++
Sbjct: 327 EVHLALDEMPHIEKCNCPPGFNGLSCESCESGYRRVNNVLYQ----GQCEACACYNHSNTC--DSL---------NGECQNCTHNTAGAHCEDCASGYYGD--AKSKNLNFCKKCPCPRLDYQMTELCSSYSSENYVCHK----CLHNTSGVHCDRCIEGYYG-NPKKKISCKRCKC----NNNQESCDPVTGECRCSYNTMGTECQLCKHGFY--GDALTKSCKKCDCDSLGST-SENCDKLTGECDCRTNFVNRQCDSCTSGLYLK 565 HSP 9 Score: 86.2705 bits (212), Expect = 2.131e-16 Identity = 66/207 (31.88%), Postives = 90/207 (43.48%), Query Frame = 3
Query: 2367 KCLCREGVEGQHCDTCKPGYWNFPF--------------------CHSCKCNQMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSGNIDQRIEDSCNSKTGECRKCLFNTSGFNCEKCVSGYFGNGAVRTCTKC 2927
KC+C G HC GY+ F C CN S CDS TG C NC GN G CQ C G+Y + C+ CQCP S+ N + C+ I ++ C C+ GYSG+ C+ C+ +GN I K C+ C C+ Q + +CN + G C+ C G C++C Y +TC C
Sbjct: 1083 KCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVCDSVTGDCQNCMGNREGNKCQLCREGFYKNDKNYDQCEPCQCP--SAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPI-KGIPCKSCDCNQQGSQSL--NCN-EFGICQ-CSPGVLGIKCDQCAPNYVVEK--KTCQSC 1280 HSP 10 Score: 85.1149 bits (209), Expect = 4.777e-16 Identity = 52/140 (37.14%), Postives = 69/140 (49.29%), Query Frame = 3
Query: 2220 GQCQCLPNVSGKTCERCASESYGF-DARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCKCNQMS------TTCDSKTGKCLNCKGNSFGYNCQNCENGYY-GDTSRGILCKKCQCPGLS 2615
G C C PN G C +C+ +S+ F + GCKKC C + GS ++ C +G C C+ G CD C GY+ FP C C C + T CD TG+C CK N G C+ C G + D + C C C G+S
Sbjct: 727 GICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYYGFPNCQPCSCAENGTKSNELTKCDKITGQC-QCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFGVS 865 HSP 11 Score: 84.3445 bits (207), Expect = 8.977e-16 Identity = 68/217 (31.34%), Postives = 90/217 (41.47%), Query Frame = 3
Query: 2151 KSDPIKCKCNVTGSRNNNCNKIGGQCQCLPNVSGKTCERCASESYGFDARGCKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGYWNFPFCHSCK-CN-----QMSTTCDSKTGKCLNCKGNSFGYNCQNCENGYYGDTSRGILCKKCQCPGLSSLKNNSIGCSLIFNTTAFTCMCKKGYSGQFCDQCETNHFGNAISKDGTCQPCRCSG 2783
++D + C C GS N C+ G C+C NV+ + C +C +G D GCKKC N Q N+ G C+C G C+ C W+F CK CN S C+ +G C CK FG C C GYYG + C+ C C + N C I C CK+ G C C F + + +G C C C G
Sbjct: 661 RTDCVPCNCG-RGSLNPKCDMETGNCKCAQNVARRDCSKCDVGYFGLDVTGCKKC----PPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICA-CKTAFFGSKCDRCNEGYYGFPN----CQPCSCAENGTKSNELTKCDKI----TGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFCFG 863 HSP 12 Score: 83.1889 bits (204), Expect = 2.153e-15 Identity = 96/340 (28.24%), Postives = 129/340 (37.94%), Query Frame = 3
Query: 927 VYGVCDCQHNTKGQHCEKCKD-FYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGT---------------------------------KFPS--ICIQEPEL---DLGLNAGHCICKENVEGNQCDKCK-IGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLC---------------------------KCSEYGSRDNKT-----------------CDKYSGKCLCKNGFAGIRCDEIKSEWY 1694
V G C C +NT G C+ CK FY D+ T C+KC+C+S S+ + K++G CD C+ N ++ C+ C SG Y + C+ C+C+ G +P+ C+ P D LN G C C NV CDKC+ + +W N T C+ CNC G+L N CD TG C C V DCS C +GYF L ++ GC C KCSE N+T C+K SG C CK F G +CD +Y
Sbjct: 491 VTGECRCSYNTMGTECQLCKHGFYGDA--------LTKSCKKCDCDSLGSTS-------ENCDKLTGE-CD-CRTNFVNRQCDSCTSGLYL-------KDKKCIPCNCEIQGRISSEKFCNTTSGQCFCLESVNGDRCEKCVEGYFNYPNCKKCLCSPHALSADCDLNNGKCKCGSNVINRICDKCENLHYW----NNRTDCVPCNC-GRGSL-NPKCDMETGNCKCAQNVARRDCSKCDVGYFGL---DVTGCKKCPPCYNGQVCNNEGICVCPPNTIGNLCNKCSEDSWDFNETIGCKKCNCYINGSYSNICNKISGICACKTAFFGSKCDRCNEGYY 797 HSP 13 Score: 73.9442 bits (180), Expect = 1.180e-12 Identity = 53/139 (38.13%), Postives = 66/139 (47.48%), Query Frame = 3
Query: 1173 RCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLG-LNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGND-CSTCKIGYFNLSNANINGCSLCKC 1583
+C G+YR V C C C LGT C+ E D+G G CICK+ EG CD C IG+ ++ E CL C C + G++ C CK V +D C TCK GYFNL +AN GC C C
Sbjct: 10 KCIKGFYRPWNVLATELYPCTPCQCHGLGT--IGSCVSNGE-DIGDQKPGDCICKKGYEGVNCDLCSIGYERYPNGE---CLPCPCSIDGSVRT-SFYRCKPPCNCKVHVNSDDLCKTCKNGYFNLDSANPEGCEPCYC 141 HSP 14 Score: 58.5362 bits (140), Expect = 6.104e-8 Identity = 65/228 (28.51%), Postives = 88/228 (38.60%), Query Frame = 3
Query: 921 EMVYGVCDCQHNTKGQHCEKCKDFYNDSPWLPADKNSTNECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDN-----CQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQEPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENR--GCDSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKC 1583
+M G C C N + C KC D + D C+KC P Y +G VC+N C NT C +C + N + C KC+C G+ + +IC + +G C CK G++CD+C G++ F C C+C GT N CD TG+C CK V G C C+ G F GC C C
Sbjct: 679 DMETGNCKCAQNVARRDCSKC-----DVGYFGLD---VTGCKKC------------PPCY------NGQVCNNEGICVCPPNTIGNLCNKCSEDSW-----DFNETIGCKKCNCYINGS-YSNICNKI--------SGICACKTAFFGSKCDRCNEGYYGF-----PNCQPCSCAENGTKSNELTKCDKITGQCQCKQNVIGTKCKVCRKGTFPSDGTYEGGCFSCFC 861 HSP 15 Score: 55.8398 bits (133), Expect = 3.596e-7 Identity = 65/230 (28.26%), Postives = 89/230 (38.70%), Query Frame = 3
Query: 891 KAKEGQVQVKEMVYGV--CDCQHNTKGQHCE-KCKDFYNDSPWLPADKNSTN---------ECRKCECNSHSSSCHFDPVVYQQSRKMSGGVCDNCQHNTESKNCERCKSGYYRNIAVAINSSQTCLKCHCDSLGTKFPSICIQ-EPELDLGLNAGHCICKENVEGNQCDKCKIGFWKFNGNEETG--CLKCNCVMMGTLENRGCDSSTGKCYCKSFVTGNDCSTCKIGY 1535
K E + + V V C C T G HC+ +Y + LP S + +KC CN +S C D V G C NC N E C+ C+ G+Y+N + C C C S F C + E L+L C C+ G C+ C+ GF+ GN G C C+C G+ ++ C+ G C C V G C C Y
Sbjct: 1066 KVMETTQAIGKRVTAVEKCICNALTFGNHCQNPISGYYKEYFNLPNSTKSGSMRTGNRILINLKKCNCNGYSKVC--DSVT---------GDCQNCMGNREGNKCQLCREGFYKNDK----NYDQCEPCQCPSAHYNFAQNCTRIESSLEL-----KCQCRIGYSGKSCENCQQGFY---GNPIKGIPCKSCDCNQQGS-QSLNCN-EFGICQCSPGVLGIKCDQCAPNY 1270 HSP 16 Score: 54.6842 bits (130), Expect = 9.859e-7 Identity = 34/81 (41.98%), Postives = 41/81 (50.62%), Query Frame = 3
Query: 2202 NCNKIGG----QCQCLPNVSGKTCERCASESYGFDARG--CKKCKCDLTGSLNSQCYNDTGKCLCREGVEGQHCDTCKPGY 2426
NC +I +CQC SGK+CE C YG +G CK C C+ GS + C N+ G C C GV G CD C P Y
Sbjct: 1191 NCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGIPCKSCDCNQQGSQSLNC-NEFGICQCSPGVLGIKCDQCAPNY 1270 HSP 17 Score: 53.1434 bits (126), Expect = 2.636e-6 Identity = 41/131 (31.30%), Postives = 63/131 (48.09%), Query Frame = 3
Query: 1329 CKENVEGNQCDKCKIGFWKFNGNEETGCLKCNCVMMGTLENRGC----DSSTGKCYCKSFVTGNDCSTCKIGYFNLSNANINGCSLCKCSEYGSRDNKTCDKYSGKCLCKNGFAGIRCDEIKSEWYIEKPT 1709
C N EGN+C C+ GF+K + N + C C C + C S KC C+ +G C C+ G++ I C C C++ GS+ + C+++ G C C G GI+CD+ + +EK T
Sbjct: 1150 CMGNREGNKCQLCREGFYKNDKNYDQ-CEPCQCPSAHYNFAQNCTRIESSLELKCQCRIGYSGKSCENCQQGFYGNPIKGI-PCKSCDCNQQGSQ-SLNCNEF-GICQCSPGVLGIKCDQCAPNYVVEKKT 1276 The following BLAST results are available for this feature:
BLAST of Netrin-4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Netrin-4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Netrin-4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Netrin-4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Netrin-4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Netrin-4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009281 ID=SMED30009281|Name=Netrin-4|organism=Schmidtea mediterranea sexual|type=transcript|length=5223bpback to top protein sequence of SMED30009281-orf-1 >SMED30009281-orf-1 ID=SMED30009281-orf-1|Name=SMED30009281-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1689bp MTLNYVFIIFVARYILEIESDNVCRKTICNPRAGDLLIGRESFLRASSTCback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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