SMED30009274

Overview
NameSMED30009274
Smed IDSMED30009274
Length (bp)1268
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009274 (SMED30009274) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009274 (SMED30009274) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009274 (SMED30009274) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009274 (SMED30009274) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30009274

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 31

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30009274h1SMcG0023080 SmedASXL_018632SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0003610 Contig39361uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0003610 Contig39361newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0004116 Contig14292uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0004116 Contig14292newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig34357uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig34357newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig28971newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig28971uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig12708uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig12708newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig46250uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig46250newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30009274h1SMcG0023080 dd_Smed_v4_4433_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig50537newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30009274h1SMcG0023080 Contig50537uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30009274h1SMcG0023080 dd_Smed_v4_5235_0_7dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30009274h1SMcG0023080 dd_Smed_v4_5235_0_7dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30009274h1SMcG0023080 dd_Smed_v4_990_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30009274h1SMcG0023080 dd_Smed_v4_990_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30009274h1SMcG0023080 dd_Smed_v4_990_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30009274h1SMcG0023080 dd_Smed_v4_990_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009274h1SMcG0023080 dd_Smed_v4_990_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30009274h1SMcG0023080 dd_Smed_v4_990_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30009274h1SMcG0023080 dd_Smed_v4_990_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009274h1SMcG0023080 dd_Smed_v6_5235_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30009274h1SMcG0023080 dd_Smed_v6_990_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30009274h1SMcG0023080 dd_Smed_v6_990_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle progenitor cellSMED30009274h1SMcG0023080 dd_Smed_v6_990_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30009274h1SMcG0023080 dd_Smed_v4_4433_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30009274h1SMcG0023080 dd_Smed_v4_4433_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009274 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000150:106037..109698 +6123
Homology
BLAST of SMED30009274 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 97.0561 bits (240), Expect = 5.745e-25
Identity = 57/153 (37.25%), Postives = 94/153 (61.44%), Query Frame = -1
Query:  627 HVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1076
            H DA+  D  YDH AFLG EEA+ + +LTPEESK RL +++ +++D +KDG VT  ELKDWIKF  K+W+ ++ +R W  ++      + +  Y    +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   47 HNDAQSFD--YDHDAFLGAEEAKTFDQLTPEESKERLGKIV-SKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 192          

HSP 2 Score: 37.7354 bits (86), Expect = 5.745e-25
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  196 MKDIVVQETMEDIDKNADGFIDLEEYIGDMY 226          
BLAST of SMED30009274 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 97.0561 bits (240), Expect = 5.843e-25
Identity = 57/153 (37.25%), Postives = 94/153 (61.44%), Query Frame = -1
Query:  627 HVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1076
            H DA+  D  YDH AFLG EEA+ + +LTPEESK RL +++ +++D +KDG VT  ELKDWIKF  K+W+ ++ +R W  ++      + +  Y    +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   39 HNDAQSFD--YDHDAFLGAEEAKTFDQLTPEESKERLGKIV-SKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 37.7354 bits (86), Expect = 5.843e-25
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  188 MKDIVVQETMEDIDKNADGFIDLEEYIGDMY 218          
BLAST of SMED30009274 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 95.1301 bits (235), Expect = 2.290e-24
Identity = 55/153 (35.95%), Postives = 93/153 (60.78%), Query Frame = -1
Query:  627 HVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1076
            H DA+  D  YDH AFLG EEA+ + +LTPEESK RL  ++ +++D +KDG VT+ ELK WIK   KK++ D  +  W +++      + ++ Y  + +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   39 HNDAQSFD--YDHDAFLGAEEAKTFDQLTPEESKERLGMIV-DKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTYLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 37.7354 bits (86), Expect = 2.290e-24
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  188 MKDIVVQETMEDIDKNADGFIDLEEYIGDMY 218          
BLAST of SMED30009274 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 94.7449 bits (234), Expect = 2.465e-24
Identity = 55/153 (35.95%), Postives = 93/153 (60.78%), Query Frame = -1
Query:  627 HVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1076
            H DA+  D  YDH AFLG EEA+ + +LTPEESK RL  ++ +++D +KDG VT+ ELK WIK   KK++ D  +  W +++      + ++ Y  + +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   47 HNDAQSFD--YDHDAFLGAEEAKTFDQLTPEESKERLGMIV-DKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTYLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 192          

HSP 2 Score: 37.7354 bits (86), Expect = 2.465e-24
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  196 MKDIVVQETMEDIDKNADGFIDLEEYIGDMY 226          
BLAST of SMED30009274 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 97.0561 bits (240), Expect = 6.674e-24
Identity = 57/153 (37.25%), Postives = 94/153 (61.44%), Query Frame = -1
Query:  627 HVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1076
            H DA+  D  YDH AFLG EEA+ + +LTPEESK RL +++ +++D +KDG VT  ELKDWIKF  K+W+ ++ +R W  ++      + +  Y    +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   39 HNDAQSFD--YDHDAFLGAEEAKTFDQLTPEESKERLGKIV-SKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 34.2686 bits (77), Expect = 6.674e-24
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = -2
Query:  533 MRPFLIDEMLDRADKNHDGYIDLEEYTG 616
            M+  ++ E ++  DKN DG+IDLEEY G
Sbjct:  188 MKDIVVQETMEDIDKNADGFIDLEEYIG 215          
BLAST of SMED30009274 vs. Ensembl Celegans
Match: calu-1 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:H2L0I1])

HSP 1 Score: 90.1225 bits (222), Expect = 9.609e-23
Identity = 54/167 (32.34%), Postives = 95/167 (56.89%), Query Frame = -1
Query:  627 GSTRQRDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKT--QLDFNGYSEINFG--DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1115
             S    D S   +H   + HD  YDH+ FLGK+ A ++ ELTPE+SK +L +L+  ++D + DG + ++ELKD I F+ K+++ ++ DR W +Y  +     ++ +  Y E+ +G  D   +    +  K + +D     ++W +AD + +G LDR E+  F+ PE+
Sbjct:   16 ASHHSSDPSKDGEHFKGKEHDKKYDHEQFLGKDTAAEFDELTPEKSKEKLAKLVP-KMDADSDGFIEENELKDHINFMQKRYVNNDVDRTWKNYKAEKIVDGKIKWEDYREMVYGSADGAGQELSPEYAKMIARDE----KRWAVADYDSNGALDRTEYGCFMHPED 177          

HSP 2 Score: 35.8094 bits (81), Expect = 9.609e-23
Identity = 15/31 (48.39%), Postives = 22/31 (70.97%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            MR  ++ E +D  DKN DG +DL+EY G+M+
Sbjct:  181 MRDVVVAETVDDIDKNKDGSVDLDEYIGDMY 211          
BLAST of SMED30009274 vs. Ensembl Celegans
Match: calu-1 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:H2L0I1])

HSP 1 Score: 90.1225 bits (222), Expect = 9.609e-23
Identity = 54/167 (32.34%), Postives = 95/167 (56.89%), Query Frame = -1
Query:  627 GSTRQRDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKT--QLDFNGYSEINFG--DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1115
             S    D S   +H   + HD  YDH+ FLGK+ A ++ ELTPE+SK +L +L+  ++D + DG + ++ELKD I F+ K+++ ++ DR W +Y  +     ++ +  Y E+ +G  D   +    +  K + +D     ++W +AD + +G LDR E+  F+ PE+
Sbjct:   16 ASHHSSDPSKDGEHFKGKEHDKKYDHEQFLGKDTAAEFDELTPEKSKEKLAKLVP-KMDADSDGFIEENELKDHINFMQKRYVNNDVDRTWKNYKAEKIVDGKIKWEDYREMVYGSADGAGQELSPEYAKMIARDE----KRWAVADYDSNGALDRTEYGCFMHPED 177          

HSP 2 Score: 35.8094 bits (81), Expect = 9.609e-23
Identity = 15/31 (48.39%), Postives = 22/31 (70.97%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            MR  ++ E +D  DKN DG +DL+EY G+M+
Sbjct:  181 MRDVVVAETVDDIDKNKDGSVDLDEYIGDMY 211          
BLAST of SMED30009274 vs. Ensembl Fly
Match: scf (gene:FBgn0025682 transcript:FBtr0089942)

HSP 1 Score: 103.99 bits (258), Expect = 1.253e-26
Identity = 58/158 (36.71%), Postives = 95/158 (60.13%), Query Frame = -1
Query:  627 QHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG-------DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1079
            +H D   H+  +DH+AFLG +E++K+  LTPEES+ RL  +I +R+D NKDG VT +ELK+WI +  ++++ ++  RVW  +NP N   + ++ Y +  +G       D+ E+       K +LK    D  +W +AD + D  L ++EF AF+ PE+
Sbjct:   44 RHFDGGEHNAQFDHEAFLGPDESKKFDSLTPEESRRRLG-VIVDRIDENKDGSVTLAELKNWIAYTQRRYIEEDVGRVWKQHNPDNNETISWDSYMQTVYGFMDDLSPDEKEQEENGVSYKSLLK---RDRYRWSVADQDLDDNLTKDEFTAFLHPED 197          

HSP 2 Score: 35.4242 bits (80), Expect = 1.253e-26
Identity = 18/48 (37.50%), Postives = 27/48 (56.25%), Query Frame = -2
Query:  509 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMFDCAMA 652
            FLH  D    P M+  ++ E +   DK+HDG I ++EY G+M+    A
Sbjct:  192 FLHPEDH---PSMKGVVLRETITDLDKDHDGKISVDEYIGDMYRSTGA 236          
BLAST of SMED30009274 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301907)

HSP 1 Score: 62.003 bits (149), Expect = 3.210e-10
Identity = 52/163 (31.90%), Postives = 84/163 (51.53%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLG-KEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG--------DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D   H +   H+V +DH+A +G  +EA+++  L+P+ESK RL  LI+  +D NKD  + + ELK WI    KK   +E    + + +     ++ +  Y +  +         + I+     DE K + +D+E+    +  AD NKDG+L  EEF  F  PEE
Sbjct:   54 DAHHHGEDGEHNVEFDHEAIIGNTKEAQEFDSLSPDESKRRLLILIK-MMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQDKEM----FNAADTNKDGVLTLEEFVLFQNPEE 211          
BLAST of SMED30009274 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301905)

HSP 1 Score: 62.003 bits (149), Expect = 3.210e-10
Identity = 52/163 (31.90%), Postives = 84/163 (51.53%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLG-KEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG--------DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D   H +   H+V +DH+A +G  +EA+++  L+P+ESK RL  LI+  +D NKD  + + ELK WI    KK   +E    + + +     ++ +  Y +  +         + I+     DE K + +D+E+    +  AD NKDG+L  EEF  F  PEE
Sbjct:   54 DAHHHGEDGEHNVEFDHEAIIGNTKEAQEFDSLSPDESKRRLLILIK-MMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQDKEM----FNAADTNKDGVLTLEEFVLFQNPEE 211          
BLAST of SMED30009274 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0079033)

HSP 1 Score: 62.003 bits (149), Expect = 3.210e-10
Identity = 52/163 (31.90%), Postives = 84/163 (51.53%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLG-KEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG--------DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D   H +   H+V +DH+A +G  +EA+++  L+P+ESK RL  LI+  +D NKD  + + ELK WI    KK   +E    + + +     ++ +  Y +  +         + I+     DE K + +D+E+    +  AD NKDG+L  EEF  F  PEE
Sbjct:   54 DAHHHGEDGEHNVEFDHEAIIGNTKEAQEFDSLSPDESKRRLLILIK-MMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQDKEM----FNAADTNKDGVLTLEEFVLFQNPEE 211          
BLAST of SMED30009274 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0079034)

HSP 1 Score: 62.003 bits (149), Expect = 3.210e-10
Identity = 52/163 (31.90%), Postives = 84/163 (51.53%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLG-KEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG--------DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D   H +   H+V +DH+A +G  +EA+++  L+P+ESK RL  LI+  +D NKD  + + ELK WI    KK   +E    + + +     ++ +  Y +  +         + I+     DE K + +D+E+    +  AD NKDG+L  EEF  F  PEE
Sbjct:   54 DAHHHGEDGEHNVEFDHEAIIGNTKEAQEFDSLSPDESKRRLLILIK-MMDLNKDEFIDRHELKAWILRSFKKLSEEEAADRFEEIDQDADERITWKEYLQDTYAMEDEDFKKETIDYDSYEDEQKMIKQDKEM----FNAADTNKDGVLTLEEFVLFQNPEE 211          
BLAST of SMED30009274 vs. Ensembl Zebrafish
Match: calua (calumenin a [Source:NCBI gene;Acc:415241])

HSP 1 Score: 104.375 bits (259), Expect = 6.951e-27
Identity = 57/142 (40.14%), Postives = 93/142 (65.49%), Query Frame = -1
Query:  627 YDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPAD--ELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1046
            YDH AFLG+EEA+ + +LTPEESK RL +++E ++D ++DG VT++ELK WIK   KK++ D  +R W D++  N   + +  Y  + +G  +++  P D    K+++     D +++++AD N D + D+EEF AF+ PEE
Sbjct:   49 YDHDAFLGEEEAKTFDDLTPEESKNRLGKIVE-KIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTYLDDPEPDDGYNYKQMMA---RDERRFKMADGNGDHIADKEEFTAFLHPEE 186          

HSP 2 Score: 36.1946 bits (82), Expect = 6.951e-27
Identity = 18/44 (40.91%), Postives = 29/44 (65.91%), Query Frame = -2
Query:  521 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 652
            FLH    ++   M+  ++ E ++  DKN DG+IDLEEY G+M++
Sbjct:  181 FLH---PEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMYN 221          
BLAST of SMED30009274 vs. Ensembl Zebrafish
Match: calua (calumenin a [Source:NCBI gene;Acc:415241])

HSP 1 Score: 104.375 bits (259), Expect = 6.951e-27
Identity = 57/142 (40.14%), Postives = 93/142 (65.49%), Query Frame = -1
Query:  627 YDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPAD--ELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1046
            YDH AFLG+EEA+ + +LTPEESK RL +++E ++D ++DG VT++ELK WIK   KK++ D  +R W D++  N   + +  Y  + +G  +++  P D    K+++     D +++++AD N D + D+EEF AF+ PEE
Sbjct:   49 YDHDAFLGEEEAKTFDDLTPEESKNRLGKIVE-KIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTYLDDPEPDDGYNYKQMMA---RDERRFKMADGNGDHIADKEEFTAFLHPEE 186          

HSP 2 Score: 36.1946 bits (82), Expect = 6.951e-27
Identity = 18/44 (40.91%), Postives = 29/44 (65.91%), Query Frame = -2
Query:  521 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 652
            FLH    ++   M+  ++ E ++  DKN DG+IDLEEY G+M++
Sbjct:  181 FLH---PEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMYN 221          
BLAST of SMED30009274 vs. Ensembl Zebrafish
Match: rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-040625-175])

HSP 1 Score: 95.9005 bits (237), Expect = 2.004e-24
Identity = 54/155 (34.84%), Postives = 94/155 (60.65%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDR-ELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D+  H   + H   +DH+AFLGKEE++ + +L+PEESK RL +++ +++DT+KDG V+ +EL  WIK   ++++ +  D+ WN+Y+     ++ +  Y    +G  I+  +   + K   K     D ++++ AD + DG+  REEF AF+ PEE
Sbjct:   31 DLSDHPHDDAHGFQFDHEAFLGKEESKTFDQLSPEESKDRLGKIV-DKIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWNEYDQNKDGKIGWIEYKNTTYGYYIDTEFDDVDDKATYKSMLNRDERRFKSADRDGDGVATREEFTAFLHPEE 184          

HSP 2 Score: 36.1946 bits (82), Expect = 2.004e-24
Identity = 16/32 (50.00%), Postives = 23/32 (71.88%), Query Frame = -2
Query:  521 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 616
            MR  +I E ++  DKN DG IDL+EY G+M++
Sbjct:  188 MRDIVIQETIEDIDKNGDGKIDLQEYIGDMYN 219          
BLAST of SMED30009274 vs. Ensembl Zebrafish
Match: rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-040625-175])

HSP 1 Score: 95.9005 bits (237), Expect = 2.004e-24
Identity = 54/155 (34.84%), Postives = 94/155 (60.65%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDR-ELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D+  H   + H   +DH+AFLGKEE++ + +L+PEESK RL +++ +++DT+KDG V+ +EL  WIK   ++++ +  D+ WN+Y+     ++ +  Y    +G  I+  +   + K   K     D ++++ AD + DG+  REEF AF+ PEE
Sbjct:   31 DLSDHPHDDAHGFQFDHEAFLGKEESKTFDQLSPEESKDRLGKIV-DKIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWNEYDQNKDGKIGWIEYKNTTYGYYIDTEFDDVDDKATYKSMLNRDERRFKSADRDGDGVATREEFTAFLHPEE 184          

HSP 2 Score: 36.1946 bits (82), Expect = 2.004e-24
Identity = 16/32 (50.00%), Postives = 23/32 (71.88%), Query Frame = -2
Query:  521 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 616
            MR  +I E ++  DKN DG IDL+EY G+M++
Sbjct:  188 MRDIVIQETIEDIDKNGDGKIDLQEYIGDMYN 219          
BLAST of SMED30009274 vs. Ensembl Zebrafish
Match: rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-040625-175])

HSP 1 Score: 96.2857 bits (238), Expect = 2.037e-24
Identity = 54/155 (34.84%), Postives = 94/155 (60.65%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDR-ELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D+  H   + H   +DH+AFLGKEE++ + +L+PEESK RL +++ +++DT+KDG V+ +EL  WIK   ++++ +  D+ WN+Y+     ++ +  Y    +G  I+  +   + K   K     D ++++ AD + DG+  REEF AF+ PEE
Sbjct:   31 DLSDHPHDDAHGFQFDHEAFLGKEESKTFDQLSPEESKDRLGKIV-DKIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWNEYDQNKDGKIGWIEYKNTTYGYYIDTEFDDVDDKATYKSMLNRDERRFKSADRDGDGVATREEFTAFLHPEE 184          

HSP 2 Score: 36.1946 bits (82), Expect = 2.037e-24
Identity = 16/32 (50.00%), Postives = 23/32 (71.88%), Query Frame = -2
Query:  521 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 616
            MR  +I E ++  DKN DG IDL+EY G+M++
Sbjct:  188 MRDIVIQETIEDIDKNGDGKIDLQEYIGDMYN 219          
BLAST of SMED30009274 vs. Ensembl Xenopus
Match: calu (calumenin [Source:NCBI gene;Acc:407965])

HSP 1 Score: 97.4413 bits (241), Expect = 3.667e-24
Identity = 57/171 (33.33%), Postives = 101/171 (59.06%), Query Frame = -1
Query:  627 SGSTRQRDFSDVEQHVDAEVHD----VNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1118
            S  T ++D    +  +  +VHD     +YDH AFLG EEA+ + +LTPEESK RL R++  ++D +KDG VT +EL+DWI F  K+W+ ++ +R W  ++    + + +  Y    +G  +++  P +     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   19 SKPTEKKDRVHHDPQLSEKVHDDAQNFDYDHDAFLGAEEAKTFDQLTPEESKERLGRIV-GKIDADKDGYVTVNELRDWIIFAQKRWIYEDVERQWKGHDLNGDSMVSWEEYKNATYGYILDDPDPDNSFNYKQMMVRDE----RRFKMADQDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 34.6538 bits (78), Expect = 3.667e-24
Identity = 15/32 (46.88%), Postives = 23/32 (71.88%), Query Frame = -2
Query:  521 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 616
            M+  ++ E ++  DKN DG IDLEEY G+M++
Sbjct:  188 MKDIVVLETMEDIDKNGDGLIDLEEYIGDMYN 219          
BLAST of SMED30009274 vs. Ensembl Xenopus
Match: calu (calumenin [Source:NCBI gene;Acc:407965])

HSP 1 Score: 90.5077 bits (223), Expect = 4.252e-22
Identity = 55/171 (32.16%), Postives = 99/171 (57.89%), Query Frame = -1
Query:  627 SGSTRQRDFSDVEQHVDAEVHD----VNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1118
            S  T ++D    +  +  +VHD     +YDH AFLG EEA+ + +LTPEESK RL  ++ +++D + DG VT+ EL  WIK   KK++ D  +R W +++      + ++ Y  + +G  +++  P +     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   19 SKPTEKKDRVHHDPQLSEKVHDDAQNFDYDHDAFLGAEEAKTFDQLTPEESKERLGMIV-SKIDLDNDGYVTEGELTAWIKKAQKKYVYDNVERQWQEFDLNQDGLVSWDEYRNVTYGTYLDDPDPDNSFNYKQMMVRDE----RRFKMADQDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 34.6538 bits (78), Expect = 4.252e-22
Identity = 15/32 (46.88%), Postives = 23/32 (71.88%), Query Frame = -2
Query:  521 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 616
            M+  ++ E ++  DKN DG IDLEEY G+M++
Sbjct:  188 MKDIVVLETMEDIDKNGDGLIDLEEYIGDMYN 219          
BLAST of SMED30009274 vs. Ensembl Xenopus
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:395023])

HSP 1 Score: 88.5817 bits (218), Expect = 6.324e-20
Identity = 54/151 (35.76%), Postives = 89/151 (58.94%), Query Frame = -1
Query:  627 YDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG-----------DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1046
            YDH+AFLGKEEA  + +LTPEES+ RL +++ +++D +KDG VT  ELK+W+K    +++ +  ++ W DY+      + +  Y    +G           DK +ERY     K +++D     +++++AD + D +  REEF AF+ PEE
Sbjct:   48 YDHEAFLGKEEARTFDQLTPEESQHRLGKIV-DKIDKDKDGFVTDIELKEWMKHSQNRYIYENVNKHWADYDKNKDDMISWEEYKNTTYGYLPGEEFYDVADKDKERY----RKMMMRDE----RRFKVADKDGDLIATREEFTAFLHPEE 189          

HSP 2 Score: 29.261 bits (64), Expect = 6.324e-20
Identity = 12/31 (38.71%), Postives = 20/31 (64.52%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  +I E ++  DKN DG +D+ EY  +M+
Sbjct:  193 MQDIVITETIEDIDKNDDGIVDVHEYIADMY 223          
BLAST of SMED30009274 vs. Ensembl Xenopus
Match: fgf11 (fibroblast growth factor 11 [Source:Xenbase;Acc:XB-GENE-479522])

HSP 1 Score: 90.1225 bits (222), Expect = 1.811e-19
Identity = 55/142 (38.73%), Postives = 85/142 (59.86%), Query Frame = -1
Query:  627 DHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKI---EERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1043
            DH+AFLGK++A+ + +LTPEESK RL ++I NR+D++ DG +T  EL  WIK + K+++ +   RVW DY+      + +  Y +  +G  +   EE   A E     K    D ++++ AD N D   +REEF +F+ PEE
Sbjct:   49 DHEAFLGKDDAKTFDQLTPEESKDRLGKII-NRIDSDSDGLITTEELTAWIKRVQKRYVYENVARVWKDYDVNKDNTISWEEYKQATYGYYLANPEEFQDAAEQFSFKKMLPRDERRFKQADLNGDLEANREEFTSFLHPEE 189          
BLAST of SMED30009274 vs. Ensembl Xenopus
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:395023])

HSP 1 Score: 84.7297 bits (208), Expect = 8.318e-19
Identity = 56/145 (38.62%), Postives = 90/145 (62.07%), Query Frame = -1
Query:  627 YDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG---DKIEERY-PADELK-RVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1046
            YDH+AFLGKEEA  + +LTPEES+ RL ++I +++D + D  +T  EL  WIK +S++W L++ ++    Y+      + ++ Y++  +G    K EE Y  AD+ K R  K    D +++++AD + D +  REEF AF+ PEE
Sbjct:   48 YDHEAFLGKEEARTFDQLTPEESQHRLGKII-DQMDKDNDKYITSGELFAWIKHVSRRWNLEDSEKQGKKYDTNKDGMISWDEYAKGVYGHLLGKGEEFYDVADKDKERYRKMMMRDERRFKVADKDGDLIATREEFTAFLHPEE 191          

HSP 2 Score: 29.261 bits (64), Expect = 8.318e-19
Identity = 12/31 (38.71%), Postives = 20/31 (64.52%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  +I E ++  DKN DG +D+ EY  +M+
Sbjct:  195 MQDIVITETIEDIDKNDDGIVDVHEYIADMY 225          
BLAST of SMED30009274 vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 94.3597 bits (233), Expect = 2.345e-24
Identity = 54/153 (35.29%), Postives = 94/153 (61.44%), Query Frame = -1
Query:  627 HVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1076
            H DA+  + +YDH AFLG EEA+ + +LTPEESK RL  ++ +++D +KDG VT+ ELK WIK   KK++ D  +  W +++      + ++ Y  + +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   39 HNDAQ--NFDYDHDAFLGAEEAKSFDQLTPEESKERLGMIV-DKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYGTYLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 37.7354 bits (86), Expect = 2.345e-24
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  188 MKDIVVQETMEDIDKNADGFIDLEEYIGDMY 218          
BLAST of SMED30009274 vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 93.2041 bits (230), Expect = 4.917e-24
Identity = 58/171 (33.92%), Postives = 100/171 (58.48%), Query Frame = -1
Query:  627 SGSTRQRDFSDVEQHVDAEVHD----VNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1118
            S  T ++D    E  +  +VH+     +YDH AFLG EEA+ + +LTPEESK RL +++ +++D +KDG VT  ELK WIKF  K+W+ ++ +R W  ++      + +  Y    +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   19 SKPTEKKDRVHHEPQLSDKVHNDAQNFDYDHDAFLGAEEAKSFDQLTPEESKERLGKIV-SKIDDDKDGFVTVDELKGWIKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 37.7354 bits (86), Expect = 4.917e-24
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  188 MKDIVVQETMEDIDKNADGFIDLEEYIGDMY 218          
BLAST of SMED30009274 vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 93.5893 bits (231), Expect = 1.759e-23
Identity = 58/171 (33.92%), Postives = 100/171 (58.48%), Query Frame = -1
Query:  627 SGSTRQRDFSDVEQHVDAEVHD----VNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1118
            S  T ++D    E  +  +VH+     +YDH AFLG EEA+ + +LTPEESK RL +++ +++D +KDG VT  ELK WIKF  K+W+ ++ +R W  ++      + +  Y    +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   19 SKPTEKKDRVHHEPQLSDKVHNDAQNFDYDHDAFLGAEEAKSFDQLTPEESKERLGKIV-SKIDDDKDGFVTVDELKGWIKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 35.8094 bits (81), Expect = 1.759e-23
Identity = 16/38 (42.11%), Postives = 22/38 (57.89%), Query Frame = -2
Query:  503 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMFDCAMACR 616
            M+  ++ E ++  DKN DG+IDLEEY G        CR
Sbjct:  188 MKDIVVQETMEDIDKNADGFIDLEEYIGWQAHQGGDCR 225          
BLAST of SMED30009274 vs. Ensembl Mouse
Match: Rcn1 (reticulocalbin 1 [Source:MGI Symbol;Acc:MGI:104559])

HSP 1 Score: 85.1149 bits (209), Expect = 9.892e-20
Identity = 50/143 (34.97%), Postives = 87/143 (60.84%), Query Frame = -1
Query:  627 YDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKI---EERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1046
            YDH+AFLGKE+++ + +L+P+ESK RL +++ +R+D++ DG VT  ELK WIK + K+++ D   +VW DY+     ++ +  Y +  +G  +    E + + +     K    D ++++ +D + D    REEF AF+ PEE
Sbjct:   52 YDHEAFLGKEDSKTFDQLSPDESKERLGKIV-DRIDSDGDGLVTTEELKLWIKRVQKRYIYDNVAKVWKDYDRDKDEKISWEEYKQATYGYYLGNPAEFHDSSDHHTFKKMLPRDERRFKASDLDGDLTATREEFTAFLHPEE 193          

HSP 2 Score: 31.187 bits (69), Expect = 9.892e-20
Identity = 16/43 (37.21%), Postives = 26/43 (60.47%), Query Frame = -2
Query:  524 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 652
            FLH    ++   M+  ++ E L+  DKN DG++D +EY  +MF
Sbjct:  188 FLH---PEEFEHMKEIVVLETLEDIDKNGDGFVDQDEYIADMF 227          
BLAST of SMED30009274 vs. Ensembl Mouse
Match: Rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:MGI Symbol;Acc:MGI:1277122])

HSP 1 Score: 72.4034 bits (176), Expect = 1.132e-17
Identity = 45/150 (30.00%), Postives = 85/150 (56.67%), Query Frame = -1
Query:  627 DVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVD--TNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFN-----GYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1055
            +  YDH+AFLG++ A+++ +L+PEES+ RL R++ +R+D   + DG V+ +EL+ WI    ++ + D     W+ Y+     ++ +       Y     G++  +   A+  K++L   E   +++++AD + D +  REE  AF+ PEE
Sbjct:   51 NFQYDHEAFLGRDVAKEFDKLSPEESQARLGRIV-DRMDLAGDSDGWVSLAELRAWIAHTQQRHIRDSVSAAWHTYDTDRDGRVGWEELRNATYGHYEPGEEFHDVEDAETYKKMLARDE---RRFRVADQDGDSMATREELTAFLHPEE 196          

HSP 2 Score: 36.965 bits (84), Expect = 1.132e-17
Identity = 17/43 (39.53%), Postives = 27/43 (62.79%), Query Frame = -2
Query:  524 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 652
            FLH    ++ P MR  ++ E L+  DKN DGY+ +EEY  +++
Sbjct:  191 FLH---PEEFPHMRDIVVAETLEDLDKNKDGYVQVEEYIADLY 230          
BLAST of SMED30009274 vs. UniProt/SwissProt
Match: sp|Q6IQP3|CALUA_DANRE (Calumenin-A OS=Danio rerio OX=7955 GN=calua PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 4.232e-26
Identity = 57/142 (40.14%), Postives = 93/142 (65.49%), Query Frame = -1
Query:  627 YDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPAD--ELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1046
            YDH AFLG+EEA+ + +LTPEESK RL +++E ++D ++DG VT++ELK WIK   KK++ D  +R W D++  N   + +  Y  + +G  +++  P D    K+++     D +++++AD N D + D+EEF AF+ PEE
Sbjct:   47 YDHDAFLGEEEAKTFDDLTPEESKNRLGKIVE-KIDADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTYLDDPEPDDGYNYKQMMA---RDERRFKMADGNGDHIADKEEFTAFLHPEE 184          

HSP 2 Score: 36.1946 bits (82), Expect = 4.232e-26
Identity = 18/44 (40.91%), Postives = 29/44 (65.91%), Query Frame = -2
Query:  521 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 652
            FLH    ++   M+  ++ E ++  DKN DG+IDLEEY G+M++
Sbjct:  179 FLH---PEEYEHMKDIVVLETMEDIDKNGDGFIDLEEYIGDMYN 219          
BLAST of SMED30009274 vs. UniProt/SwissProt
Match: sp|Q6XLQ7|CALU_RABIT (Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU PE=1 SV=2)

HSP 1 Score: 98.5969 bits (244), Expect = 7.493e-25
Identity = 60/171 (35.09%), Postives = 101/171 (59.06%), Query Frame = -1
Query:  627 SGSTRQRDFSDVEQHVDAEVHD----VNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1118
            S  T ++D    E  +  +VHD     +YDH AFLG EEA+ + +LTPEESK RL +++ +++D +KDG VT  ELKDWIKF  K+W+ ++ +R W  ++      + +  Y    +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   19 SKPTEKKDRVHHEPQLSDKVHDDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIV-SKIDADKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 37.7354 bits (86), Expect = 7.493e-25
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  188 MKDIVVQETMEDIDKNGDGFIDLEEYIGDMY 218          
BLAST of SMED30009274 vs. UniProt/SwissProt
Match: sp|O43852|CALU_HUMAN (Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 2.534e-24
Identity = 57/153 (37.25%), Postives = 94/153 (61.44%), Query Frame = -1
Query:  627 HVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1076
            H DA+  D  YDH AFLG EEA+ + +LTPEESK RL +++ +++D +KDG VT  ELKDWIKF  K+W+ ++ +R W  ++      + +  Y    +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   39 HNDAQSFD--YDHDAFLGAEEAKTFDQLTPEESKERLGKIV-SKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 37.7354 bits (86), Expect = 2.534e-24
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  188 MKDIVVQETMEDIDKNADGFIDLEEYIGDMY 218          
BLAST of SMED30009274 vs. UniProt/SwissProt
Match: sp|Q5RDD8|CALU_PONAB (Calumenin OS=Pongo abelii OX=9601 GN=CALU PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 3.379e-24
Identity = 57/153 (37.25%), Postives = 94/153 (61.44%), Query Frame = -1
Query:  627 HVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL---KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1076
            H DA+  D  YDH AFLG EEA+ + +LTPEESK RL +++ +++D +KDG VT  ELKDWIKF  K+W+ ++ +R W  ++      + +  Y    +G  +++  P D     + +++D     +++++AD + D +  +EEF AF+ PEE
Sbjct:   39 HNDAQSFD--YDHDAFLGAEEAKTFDQLTPEESKERLGKIV-SKIDDDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDE----RRFKMADKDGDLIATKEEFTAFLHPEE 184          

HSP 2 Score: 37.7354 bits (86), Expect = 3.379e-24
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDLEEY G+M+
Sbjct:  188 MKDIVVQETMEDIDKNADGFIDLEEYIGDMY 218          
BLAST of SMED30009274 vs. UniProt/SwissProt
Match: sp|B5X186|CALUA_SALSA (Calumenin-A OS=Salmo salar OX=8030 GN=calua PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 2.188e-23
Identity = 57/152 (37.50%), Postives = 96/152 (63.16%), Query Frame = -1
Query:  627 QHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELK-RVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1079
            +H DA+  D  YDH AFLG++EA+ + +L+PEESK RL  ++E ++D + DG VT+ EL+ WIK   KK++ +  DR W D++  N   + +  Y  + +G  +++  P D    + +  R  D +++++AD N+D + ++EEF AF+ PEE
Sbjct:   38 EHDDAQGFD--YDHDAFLGQKEAKSFDDLSPEESKRRLGVIVE-KIDGDSDGFVTEVELRAWIKKAQKKYIYENVDRQWKDFDVNNDGMISWEEYRNVTYGTYLDDPEPDDGYNYQHMMAR--DERRFKMADQNRDQIANKEEFTAFLHPEE 184          

HSP 2 Score: 33.8834 bits (76), Expect = 2.188e-23
Identity = 17/44 (38.64%), Postives = 28/44 (63.64%), Query Frame = -2
Query:  521 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 652
            FLH    ++   M+  ++ E ++  DKN DG+IDL EY G+M++
Sbjct:  179 FLH---PEEYDHMKDIVVLETMEDIDKNGDGFIDLNEYIGDMYN 219          
BLAST of SMED30009274 vs. TrEMBL
Match: A0A1B4XSS5 (Calumenin OS=Dugesia japonica OX=6161 GN=Djcalu PE=2 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 1.932e-86
Identity = 142/179 (79.33%), Postives = 161/179 (89.94%), Query Frame = -1
Query:  627 MIKXXXXXXXXXXXLSGSTRQRDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1163
            MIKLLL LC  V+VLSGSTRQRD SDVE H DAE+HD+NYDHKAFLGKEEAE+YSELTPEESK RLR L+EN+VD NKDGKVTKSEL DWIK +SKKWM+DERDRVWNDYNP+NK+ L+FN YS+IN+G+ IE+RY  DELKRVLK+RE+D QKWQLADDNKDGLLD EEFFA++RPEE
Sbjct:    1 MIKLLLCLCLFVSVLSGSTRQRDLSDVEHHSDAEIHDLNYDHKAFLGKEEAERYSELTPEESKNRLRILVENKVDRNKDGKVTKSELADWIKQVSKKWMMDERDRVWNDYNPQNKSHLNFNLYSDINYGENIEQRYQGDELKRVLKEREMDRQKWQLADDNKDGLLDIEEFFAYIRPEE 179          

HSP 2 Score: 64.3142 bits (155), Expect = 1.428e-7
Identity = 26/36 (72.22%), Postives = 33/36 (91.67%), Query Frame = -2
Query:  524 KKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 631
            ++VPRM+PFL+DEMLDRADKNHD YID+EEYT  ++
Sbjct:  178 EEVPRMKPFLVDEMLDRADKNHDSYIDIEEYTDELY 213          
BLAST of SMED30009274 vs. TrEMBL
Match: A0A3B4DL50 (Calumenin a OS=Pygocentrus nattereri OX=42514 PE=4 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.437e-26
Identity = 62/153 (40.52%), Postives = 103/153 (67.32%), Query Frame = -1
Query:  627 QHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPAD--ELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1079
            +H DAE  +  YDH AFLG+E A+ + +LTPEESK RL +++E ++D ++DG VT++ELK+WIK   KK++ D  DR W D++  N  ++ ++ Y  + +G  +++  P D    K+++   E   +++++AD N+DG+ D+EEF AF+ PEE
Sbjct:   83 EHNDAE--NFEYDHDAFLGEEVAKSFDDLTPEESKERLGKIVE-KIDADQDGFVTETELKEWIKKAQKKYIYDNVDRQWKDFDTNNDGRISWDEYKNVTYGTYLDDPEPDDGYNYKQMMARDE---RRFKMADRNRDGIADKEEFTAFLHPEE 229          

HSP 2 Score: 37.7354 bits (86), Expect = 1.437e-26
Identity = 19/44 (43.18%), Postives = 29/44 (65.91%), Query Frame = -2
Query:  521 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 652
            FLH    ++   M+  ++ E L+  DKN DG+IDLEEY G+M++
Sbjct:  224 FLH---PEEYEHMKEIVVLETLEDIDKNGDGFIDLEEYIGDMYN 264          
BLAST of SMED30009274 vs. TrEMBL
Match: A0A0A9WL96 (Calumenin-A OS=Lygus hesperus OX=30085 GN=calua PE=4 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 4.419e-26
Identity = 68/162 (41.98%), Postives = 98/162 (60.49%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKR--------VLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D + H     H+  YDH+AFLG EEA+ + +L PEESK RL  +I +++DTNKDG V++ EL+DWI++  +K++ D  DR W  YNP+NK  + +  YS+  +G    +     EL R         L DR+L  ++W  AD N D  L ++EFFAF+ PEE
Sbjct:   43 DEKDHYKEGAHNAEYDHEAFLG-EEAKSFQDLPPEESKKRLG-IIVDKIDTNKDGTVSRHELQDWIRYTQQKYVRDNVDRTWKIYNPENKDDMSWETYSKAVYG--FMKDLSNKELTREEDGVSYQGLYDRDL--RRWHAADSNGDNNLTKDEFFAFLHPEE 198          

HSP 2 Score: 35.039 bits (79), Expect = 4.419e-26
Identity = 17/43 (39.53%), Postives = 27/43 (62.79%), Query Frame = -2
Query:  524 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 652
            FLH    ++ PR+R  ++ E ++  DK+ DG I L EYT ++F
Sbjct:  193 FLH---PEETPRLRDLVVLETIESIDKDKDGKISLSEYTRDLF 232          
BLAST of SMED30009274 vs. TrEMBL
Match: A0A4Y2BL24 (Calumenin OS=Araneus ventricosus OX=182803 GN=Calu PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 4.686e-26
Identity = 71/170 (41.76%), Postives = 106/170 (62.35%), Query Frame = -1
Query:  621 GSTRQRD--FSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDREL---DWQKWQLADDNKDGLLDREEFFAFVRPEEGA 1115
            GS R  D   SD E+H + E H+  YDH+AFLG+EEA K+ EL+PEESK RL +++E ++D +KDG V++ ELK+WI++  KK++LD+  R W  +N   K  LD+  Y ++ +   I+E    D+   +   +E+   D ++W  AD NKD  L +EEF  F+ PEE  
Sbjct:   10 GSKRVHDKPLSD-EKHYENEAHNTQYDHEAFLGEEEARKFDELSPEESKERLGKIVE-KIDKDKDGFVSQDELKNWIEYTQKKYILDDVSRQWETHNEAKKDTLDWETYKKVTYS-FIDEDQSQDDDPDIKTYKEMMQRDKRRWMTADKNKDEQLSKEEFADFLHPEEAP 176          
BLAST of SMED30009274 vs. TrEMBL
Match: A0A087UJQ4 (Calumenin-A (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_19023 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.319e-26
Identity = 65/161 (40.37%), Postives = 102/161 (63.35%), Query Frame = -1
Query:  621 FSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDREL---DWQKWQLADDNKDGLLDREEFFAFVRPEEGA 1094
             SD + + + +VH+  YDH+AFLG+EEA K+ +L+PEESK RL +++ +++D N+DG VT+ ELKDWIK+  KK++LD+ DR W   N   K  +D++ Y +  +   I+E +  D+   +   +E+   D ++W  AD N D  L +EEF  F+ PEE  
Sbjct:   36 LSDEKHYAENDVHNTEYDHQAFLGEEEARKFDQLSPEESKERLAKIV-DKIDKNEDGFVTELELKDWIKYTQKKYILDDVDRQWAALNVDKKENIDWDSYKKATYS-FIDEEHNEDDENDIKTYKEMMKRDKRRWAAADKNSDNQLSKEEFTDFLHPEEAT 194          
BLAST of SMED30009274 vs. Ensembl Cavefish
Match: calua (calumenin [Source:NCBI gene;Acc:103043492])

HSP 1 Score: 106.301 bits (264), Expect = 1.136e-27
Identity = 60/153 (39.22%), Postives = 100/153 (65.36%), Query Frame = -1
Query:  627 QHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPAD--ELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1079
            +H D E  + +YDH AFLG+E A+ + +LTPEESK RL +++E ++D ++DG VT++ELK+WIK   KK++ D  DR W D++  N   + ++ Y  + +G  +++  P D    K+++     D +++++AD N D + D+EEF AF+ PEE
Sbjct:   38 EHNDGE--NFDYDHDAFLGEEVAKSFDDLTPEESKDRLGKIVE-KIDDDQDGFVTEAELKEWIKKAQKKYIYDNVDRQWKDFDMNNDGMISWDEYKNVTYGTYLDDPEPDDGYNYKQMMA---RDERRFKMADRNGDSIADKEEFTAFLHPEE 184          

HSP 2 Score: 36.5798 bits (83), Expect = 1.136e-27
Identity = 18/44 (40.91%), Postives = 29/44 (65.91%), Query Frame = -2
Query:  521 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMFD 652
            FLH    ++   M+  ++ E ++  DKN DG+IDLEEY G+M++
Sbjct:  179 FLH---PEEYEHMKEIVVLETMEDIDKNGDGFIDLEEYIGDMYN 219          
BLAST of SMED30009274 vs. Ensembl Cavefish
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:103045651])

HSP 1 Score: 106.686 bits (265), Expect = 3.781e-27
Identity = 59/161 (36.65%), Postives = 99/161 (61.49%), Query Frame = -1
Query:  627 RQRDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDREL-DWQKWQLADDNKDGLLDREEFFAFVRPEE 1106
             QRD SD   H   + H   YDH+AFLGKEEA+ + +LTPEESK +L +++ +R+DT+KDG V+ +EL  WIK   ++++ +  D+ W +Y+     ++ +  Y    +G  +++ +   E +   K   + D +++++AD + DG+  REEF AF+ PEE
Sbjct:   25 HQRDLSD---HAHDDAHSFQYDHEAFLGKEEAKTFDQLTPEESKAKLGKIV-DRIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWKEYDQDKDGKIAWTEYKNTTYGFYLDDEFSDVENRESYKAMLMRDERRFKVADKDGDGIASREEFTAFLHPEE 181          

HSP 2 Score: 34.6538 bits (78), Expect = 3.781e-27
Identity = 14/31 (45.16%), Postives = 22/31 (70.97%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG I+LEEY G+M+
Sbjct:  185 MKDLVVQETVEDIDKNGDGKINLEEYIGDMY 215          
BLAST of SMED30009274 vs. Ensembl Cavefish
Match: calub (calumenin-B [Source:NCBI gene;Acc:103027690])

HSP 1 Score: 90.8929 bits (224), Expect = 2.089e-22
Identity = 54/142 (38.03%), Postives = 86/142 (60.56%), Query Frame = -1
Query:  627 NYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELK-RVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1049
            +YDH+AFLG+EEA+ + +LTPEESK RL  LI +++D + D  VT  ELK WIK   K+W+ ++ DR W  ++      + +  Y    +G  +++  P D    R +  R  D +++++AD + +   D+EEF AF+ PEE
Sbjct:   46 DYDHEAFLGQEEAKTFDQLTPEESKERLG-LIVDKIDEDHDAHVTVEELKKWIKHAQKRWIYEDVDRQWQSHDLNADGYVSWEEYKNATYGYILDDSDPDDGFNYRQMMAR--DERRFKMADTDGNLQADKEEFTAFLHPEE 184          

HSP 2 Score: 34.2686 bits (77), Expect = 2.089e-22
Identity = 14/31 (45.16%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG+IDL+EY G+M+
Sbjct:  188 MKDIVVLETMEDIDKNGDGFIDLDEYIGDMY 218          
BLAST of SMED30009274 vs. Ensembl Cavefish
Match: rcn1 (reticulocalbin 1 [Source:NCBI gene;Acc:103039743])

HSP 1 Score: 88.5817 bits (218), Expect = 1.962e-20
Identity = 50/146 (34.25%), Postives = 91/146 (62.33%), Query Frame = -1
Query:  627 YDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG------DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1046
            YDH+AFLGKE+A+ + +L+P ES+ RL +++ +R+D + +G +T  ELK WIK + K+++ +   +VW+DY+     ++ ++ Y +  +G      ++ E+       K++L   E   ++++LAD + D   +REEF AF+ PEE
Sbjct:   48 YDHEAFLGKEDAKTFDQLSPAESRDRLGKIV-DRIDADGNGYITTEELKAWIKRVQKRYVYENVAKVWSDYDLNKDNKISWDEYKQATYGYYLANPEEFEDATDQFSFKKMLPRDE---RRFKLADLDGDLAAEREEFTAFLHPEE 189          

HSP 2 Score: 30.0314 bits (66), Expect = 1.962e-20
Identity = 12/31 (38.71%), Postives = 21/31 (67.74%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E ++  DKN DG++D +EY  +MF
Sbjct:  193 MKEIVVLETMEDIDKNGDGHVDEDEYIADMF 223          
BLAST of SMED30009274 vs. Ensembl Cavefish
Match: rcn2 (reticulocalbin 2 [Source:NCBI gene;Acc:103045530])

HSP 1 Score: 51.2174 bits (121), Expect = 9.562e-7
Identity = 35/150 (23.33%), Postives = 76/150 (50.67%), Query Frame = -1
Query:  627 HDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEE------RYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1058
            H + ++  AFL  E+ ++  +L+P E + ++  +++ R+DT+ D  +T  E+  WI+ + K++ +D+    +  ++  +   + ++ Y+ +  G  +E         P +E  R L  +E   +++  AD +    L+  EF AF  P E
Sbjct:   24 HHIQHNSNAFLDDEDKDEIKKLSPSEQRGKMVEIVK-RIDTDSDKFLTPEEITVWIQRVYKRYAMDDAQERFPGFDINSDGLVTWDEYNMVMHGHTVEVDENAVLEDPEEESLRFLHLKE--KRRFDFADTDGTSGLNVTEFLAFTHPSE 170          
BLAST of SMED30009274 vs. Ensembl Sea Lamprey
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 85.5001 bits (210), Expect = 1.914e-22
Identity = 51/145 (35.17%), Postives = 85/145 (58.62%), Query Frame = -1
Query:  627 NYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFG----DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1049
            +YDH+AFLG E A+ + +L+PEESK RL +++E ++D++ DG VT+ E+  WIK   ++W+ ++ DR W  ++      + +  Y    +G      IEE       K++L   E   ++++ AD + DG   +EEF AF+ PEE
Sbjct:   48 DYDHEAFLGAETAKTFDQLSPEESKERLGKIVE-KIDSDGDGLVTQDEMTMWIKHTQRRWVYEDVDRQWTGHDIDGDGSVSWEEYRNATYGFLAVSPIEE--DGFNYKQMLGRDE---RRFKAADVDGDGQASKEEFTAFLHPEE 186          

HSP 2 Score: 37.3502 bits (85), Expect = 1.914e-22
Identity = 18/46 (39.13%), Postives = 29/46 (63.04%), Query Frame = -2
Query:  515 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMFDCA 652
            FLH    ++   M+  ++ E ++  DKN DG+IDL EY G+M++ A
Sbjct:  181 FLH---PEEFEHMKDIIVTETMEDIDKNGDGFIDLNEYIGDMYNQA 223          
BLAST of SMED30009274 vs. Ensembl Sea Lamprey
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 61.6178 bits (148), Expect = 1.623e-15
Identity = 36/113 (31.86%), Postives = 61/113 (53.98%), Query Frame = -1
Query:  627 LIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEE--RYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 959
            +I  R+D N DG VT++EL +WI+   ++W+L+E +R W+ ++      +++  Y    +    EE    P      VL+D     +++Q AD + DG   R E+ AF+ PEE
Sbjct:    1 MIVTRIDENGDGLVTETELTEWIRHAQRRWVLEEAERQWSSHDLDGDGYVNWAEYKNATYSYLAEEDPEDPDGYRAMVLRDE----RRFQAADVDGDGRASRNEYAAFLHPEE 109          

HSP 2 Score: 37.7354 bits (86), Expect = 1.623e-15
Identity = 17/43 (39.53%), Postives = 29/43 (67.44%), Query Frame = -2
Query:  524 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 652
            FLH    ++ P MR  ++ E ++  DKN DG+I+++EY G+M+
Sbjct:  104 FLH---PEEHPHMRNLVVTETIEDIDKNGDGFINVDEYIGDMY 143          
BLAST of SMED30009274 vs. Ensembl Nematostella
Match: EDO28554 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T654])

HSP 1 Score: 96.2857 bits (238), Expect = 3.042e-23
Identity = 63/151 (41.72%), Postives = 91/151 (60.26%), Query Frame = -1
Query:  630 QHVDAEV-HDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPE 1079
            +H D E  H+ +YDH+AFLG E+ E++  L PEE+K RLR LI   VD NKDG VT  EL DW+K + KK  ++  D    + +     ++D+N YS+  +GD+ E+    +E+K  L+    D +++  AD NKDG L REE   F+ PE
Sbjct:   36 EHFDKEGEHNPDYDHEAFLGTEK-EEFDHLPPEEAKKRLRALI-REVDNNKDGAVTTEELTDWVKGVFKKRSMEGVDNDLKEKDANEDGKVDWNEYSKGTYGDQTEDD---EEMKEFLR---RDKRRFDAADTNKDGFLTREEMAIFLHPE 178          
BLAST of SMED30009274 vs. Ensembl Nematostella
Match: EDO35844 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKE6])

HSP 1 Score: 95.9005 bits (237), Expect = 2.883e-22
Identity = 63/151 (41.72%), Postives = 91/151 (60.26%), Query Frame = -1
Query:  630 QHVDAEV-HDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPE 1079
            +H D E  H+ +YDH+AFLG E+ E++  L PEE+K RLR LI   VD NKDG VT  EL DW+K + KK  ++  D    + +     ++D+N YS+  +GD+ E+    +E+K  L+    D +++  AD NKDG L REE   F+ PE
Sbjct:   36 EHFDKEGEHNPDYDHEAFLGTEK-EEFDHLPPEEAKKRLRALI-REVDNNKDGAVTTEELTDWVKGVFKKRSMEGVDNDLKEKDANEDGKVDWNEYSKGTYGDQTEDD---EEMKEFLR---RDKRRFDAADTNKDGFLTREEMAIFLHPE 178          
BLAST of SMED30009274 vs. Ensembl Nematostella
Match: EDO32638 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUH8])

HSP 1 Score: 79.7221 bits (195), Expect = 7.108e-21
Identity = 57/166 (34.34%), Postives = 89/166 (53.61%), Query Frame = -1
Query:  627 HDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDF----------------------NGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1058
            H+  YDH+AFLG +E + + +L+PEESK RL +L+ +++D + DGKVT+ ELK WIK  +K+++ ++ DR W+        ++                        N Y  I   DK E  Y  + +KR       D ++W+ AD N+D  L +EE+ AF+ PEE
Sbjct:   45 HNTEYDHEAFLG-DEKKTFDQLSPEESKERLGKLV-DKIDVDHDGKVTEEELKQWIKKSAKRYVYEDVDRQWDHLKKIEHAKIKMDDLVDGKRVDMAAPIGWEEYKNNTYGFIKEDDKSEYNYD-NMIKR-------DRRRWEKADINRDDKLSKEEYTAFLHPEE 200          

HSP 2 Score: 38.891 bits (89), Expect = 7.108e-21
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  +++E LD  DKN DGY+ LEEY G+++
Sbjct:  204 MKDVVVEETLDDIDKNKDGYVSLEEYLGDLY 234          
BLAST of SMED30009274 vs. Ensembl Nematostella
Match: EDO47673 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLD6])

HSP 1 Score: 90.1225 bits (222), Expect = 2.340e-20
Identity = 62/168 (36.90%), Postives = 94/168 (55.95%), Query Frame = -1
Query:  633 SDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVW---------------NDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRP 1091
            SD E      VH+  YDH AFLG+++ E +  LTP+ESK RL  LI +R+D ++DG++T +E+K+WI +   ++  D+ DR+W                 +NP     +D++ Y E  FG  I +   A+E KR  +DR    ++W++AD NKD  L +EEF A   P
Sbjct:   10 SDKEHFSKTGVHNPEYDHDAFLGQDKKE-FDSLTPQESKRRLG-LIVDRIDKDQDGRITYTEMKEWINYAGNRYAFDDGDRLWKAIKKREEQIAKKENKSFNP--DAPIDWDLYKEDAFGKTITD---ANE-KRYQRDR----KQWEVADRNKDTKLSKEEFPALFHP 165          
BLAST of SMED30009274 vs. Ensembl Nematostella
Match: EDO42789 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0S7])

HSP 1 Score: 81.2629 bits (199), Expect = 1.803e-17
Identity = 54/149 (36.24%), Postives = 89/149 (59.73%), Query Frame = -1
Query:  627 VHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDE-RDRVWNDYNPKNKTQLDFNGYSEINFG---DKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1061
            +H  + DH AFLG+E A+++  L+PEE++ RLR L+  R+D NKDG V ++EL+ WI+   KKW ++E  D ++ D +  N     +  +    +G   + I +++  + LK  ++D   D +KW+ AD +KD  L REE+  F  P+E
Sbjct:    1 MHSAHDDHVAFLGEEMAKEFENLSPEEARRRLRMLVP-RIDINKDGFVEEAELEIWIRHKMKKWEVEEDVDAIFRDMDFNNDNHATWKEFMMRTYGFTDEDINKKWERNNLKDYIED---DRRKWKYADQDKDSRLTREEYEYFHHPKE 145          
BLAST of SMED30009274 vs. Ensembl Medaka
Match: rcn3 (reticulocalbin-1 [Source:NCBI gene;Acc:101175408])

HSP 1 Score: 107.842 bits (268), Expect = 9.655e-28
Identity = 60/155 (38.71%), Postives = 93/155 (60.00%), Query Frame = -1
Query:  627 DVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDR-ELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1088
            D+  HV  + H   YDH+AFLGKEEA+ + +LTPEESK RL +++E R+DT+KDG ++ +EL  WIK   ++++ D  ++ W DY+     Q+ +  Y    +G  + E +   E K   K     D ++++ AD + DG+  R EF AF+ PEE
Sbjct:   33 DLSSHVHDDAHGYQYDHEAFLGKEEAKTFDQLTPEESKARLAKIVE-RIDTDKDGFISHAELHHWIKHRQRRYIEDNVNKHWKDYDKNRDDQIAWEEYKNTTYGYYLGEDFDDVEDKATYKSMLARDERRFRAADRDADGIATRSEFTAFLHPEE 186          

HSP 2 Score: 35.4242 bits (80), Expect = 9.655e-28
Identity = 15/31 (48.39%), Postives = 21/31 (67.74%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            M+  ++ E L+  DKN DG ID+ EY G+MF
Sbjct:  190 MKDVVVQETLEDIDKNKDGKIDINEYIGDMF 220          
BLAST of SMED30009274 vs. Ensembl Medaka
Match: calub (calumenin [Source:NCBI gene;Acc:101165303])

HSP 1 Score: 96.6709 bits (239), Expect = 3.186e-24
Identity = 58/153 (37.91%), Postives = 96/153 (62.75%), Query Frame = -1
Query:  627 QHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADEL--KRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1079
            +H DA+  + +YDH+AFLG+EEA+ + +LTPEESK RL  L+ +R+D +KDG VT  E+K WIK   K+W+ D+ DR W  ++      + +  Y    +G  ++++ P D    K+++     D +++++AD + D   ++EEF AF+ PEE
Sbjct:   41 EHDDAD--NFDYDHEAFLGQEEAKSFDQLTPEESKERLGMLV-DRIDEDKDGYVTAEEMKRWIKDAQKRWIYDDVDRQWKSHDLNGDGFVSWEEYKNATYGYVLDDQDPDDGFSYKQMMA---RDERRFKMADQDNDMKANKEEFTAFLHPEE 187          

HSP 2 Score: 34.6538 bits (78), Expect = 3.186e-24
Identity = 17/43 (39.53%), Postives = 28/43 (65.12%), Query Frame = -2
Query:  524 FLHLFDQKKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 652
            FLH    ++   M+  ++ E ++  DKN DG+IDL+EY G+M+
Sbjct:  182 FLH---PEEYEHMKDIVVLETMEDIDKNGDGFIDLDEYIGDMY 221          
BLAST of SMED30009274 vs. Ensembl Medaka
Match: rcn1 (reticulocalbin 1 [Source:NCBI gene;Acc:101175141])

HSP 1 Score: 98.2117 bits (243), Expect = 2.186e-22
Identity = 56/146 (38.36%), Postives = 93/146 (63.70%), Query Frame = -1
Query:  627 YDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIE--ERYPADE----LKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1046
            YDH+AFLGKEEA+ + +LTPEES+ RL +++E R+D + DG +T +ELKDWIK + K+++ +   +VW DY+     ++ ++ Y +  +G  +   E +  D+     K++L     D ++++ AD N D   D+EEF +F+ PEE
Sbjct:   79 YDHEAFLGKEEAKTFDQLTPEESRDRLGKIVE-RIDGDGDGYITTAELKDWIKRVQKRYVYENVAKVWTDYDLNKDNRISWDEYKQATYGYYLANPEEFQDDKDQFSFKKMLP---RDERRFKAADLNGDNTADKEEFTSFLHPEE 220          
BLAST of SMED30009274 vs. Ensembl Medaka
Match: pstpip1a (proline-serine-threonine phosphatase-interacting protein 1 [Source:NCBI gene;Acc:101156493])

HSP 1 Score: 53.9138 bits (128), Expect = 1.297e-7
Identity = 40/162 (24.69%), Postives = 80/162 (49.38%), Query Frame = -1
Query:  627 VEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDW---------QKWQLADDNKDGLLDREEFFAFVRPEE 1085
            ++ H + + H+ ++D    LG E  E+  +LTPEE + +L  +++ ++DTN D  +   E+  WI+ + +K+ L++ +  + +++      L +  Y+ +      ++ +  DE   VL+D E D          +++  AD +    L   EF AF  P E
Sbjct:   25 LQDHFNGDQHNPDHDMAVLLGDETTEEIKKLTPEEQREKLLEIVK-KIDTNGDNLLGAEEITLWIQHVYRKYALEDAEERFPEFDLNKDGVLTWEEYNTVAH----DQLFTFDE-STVLEDPEQDSLRQLHLKEKKRFDFADIDDTPGLSVSEFLAFTHPSE 180          
BLAST of SMED30009274 vs. Ensembl Medaka
Match: pstpip1a (proline-serine-threonine phosphatase-interacting protein 1 [Source:NCBI gene;Acc:101156493])

HSP 1 Score: 53.1434 bits (126), Expect = 3.992e-7
Identity = 40/162 (24.69%), Postives = 80/162 (49.38%), Query Frame = -1
Query:  627 VEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDW---------QKWQLADDNKDGLLDREEFFAFVRPEE 1085
            ++ H + + H+ ++D    LG E  E+  +LTPEE + +L  +++ ++DTN D  +   E+  WI+ + +K+ L++ +  + +++      L +  Y+ +      ++ +  DE   VL+D E D          +++  AD +    L   EF AF  P E
Sbjct:   25 LQDHFNGDQHNPDHDMAVLLGDETTEEIKKLTPEEQREKLLEIVK-KIDTNGDNLLGAEEITLWIQHVYRKYALEDAEERFPEFDLNKDGVLTWEEYNTVAH----DQLFTFDE-STVLEDPEQDSLRQLHLKEKKRFDFADIDDTPGLSVSEFLAFTHPSE 180          
BLAST of SMED30009274 vs. Planmine SMEST
Match: SMESG000007225.1 (SMESG000007225.1)

HSP 1 Score: 330.487 bits (846), Expect = 1.869e-111
Identity = 178/179 (99.44%), Postives = 178/179 (99.44%), Query Frame = -1
Query:  627 MIKXXXXXXXXXXXLSGSTRQRDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1163
            MIKLLLFLCFLVAVLSGSTRQRDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELT EESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE
Sbjct:    1 MIKLLLFLCFLVAVLSGSTRQRDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTLEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 179          

HSP 2 Score: 66.6254 bits (161), Expect = 5.208e-12
Identity = 29/36 (80.56%), Postives = 33/36 (91.67%), Query Frame = -2
Query:  524 KKVPRMRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 631
            ++VPRMRPFLIDEMLDRADKNHD YIDLEEYT  ++
Sbjct:  178 EEVPRMRPFLIDEMLDRADKNHDSYIDLEEYTDELY 213          
BLAST of SMED30009274 vs. Planmine SMEST
Match: SMESG000076911.1 (SMESG000076911.1)

HSP 1 Score: 110.153 bits (274), Expect = 4.726e-27
Identity = 66/167 (39.52%), Postives = 101/167 (60.48%), Query Frame = -1
Query:  627 STRQRDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKD-----RELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1112
            S R +D + +E+H+    HD + DHK+FLG EE++ + +L PEESK RL++LI  +VDT+KDG VT  EL+ W+  ++K     ERD+++  ++      +D+  YS+  +G        AD L  V K      ++ D +KW+LAD+N+DG LDR EF  F  PE+
Sbjct:   23 SHRIKDVNSIEEHLSNPEHDTSIDHKSFLGTEESKTFDKLLPEESKSRLKKLISEKVDTDKDGFVTVQELELWMITVAKLSKERERDKLFPFFSNSIDALMDYEEYSQGIYG-----GLQADLLDDVSKANLMIMKQQDDRKWKLADENQDGKLDRNEFLMFQHPED 184          
BLAST of SMED30009274 vs. Planmine SMEST
Match: SMESG000077404.1 (SMESG000077404.1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.923e-21
Identity = 54/148 (36.49%), Postives = 81/148 (54.73%), Query Frame = -1
Query:  627 DAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1070
            D E  D +YDH + L  EEA K+  ++P ESK +   +I+ ++D N DG V   ELK+WI  +    + D+ D  W  +  KN  +++F+ Y    FG K      AD LK + K++     +W  AD + D LL++ EFF F+ PEE
Sbjct:   38 DTENDDYSYDHNSILKSEEARKFYSMSPSESKKKFSEIID-KMDINHDGSVEMIELKNWITNVYNSILSDDVDGQWKTFGQKNDDKINFDYYVSKTFGSK----RTADHLKLINKEQ----HRWIEADLDNDKLLNKTEFFRFLHPEE 176          

HSP 2 Score: 32.3426 bits (72), Expect = 4.923e-21
Identity = 14/31 (45.16%), Postives = 18/31 (58.06%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            ++P L  E LD  D N D  ID+ EY  +MF
Sbjct:  180 LKPLLAQETLDEMDTNKDSVIDINEYLEDMF 210          
BLAST of SMED30009274 vs. Planmine SMEST
Match: SMESG000077404.1 (SMESG000077404.1)

HSP 1 Score: 87.8113 bits (216), Expect = 5.760e-21
Identity = 54/148 (36.49%), Postives = 81/148 (54.73%), Query Frame = -1
Query:  627 DAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEE 1070
            D E  D +YDH + L  EEA K+  ++P ESK +   +I+ ++D N DG V   ELK+WI  +    + D+ D  W  +  KN  +++F+ Y    FG K      AD LK + K++     +W  AD + D LL++ EFF F+ PEE
Sbjct:   26 DTENDDYSYDHNSILKSEEARKFYSMSPSESKKKFSEIID-KMDINHDGSVEMIELKNWITNVYNSILSDDVDGQWKTFGQKNDDKINFDYYVSKTFGSK----RTADHLKLINKEQ----HRWIEADLDNDKLLNKTEFFRFLHPEE 164          

HSP 2 Score: 31.9574 bits (71), Expect = 5.760e-21
Identity = 14/31 (45.16%), Postives = 18/31 (58.06%), Query Frame = -2
Query:  524 MRPFLIDEMLDRADKNHDGYIDLEEYTGNMF 616
            ++P L  E LD  D N D  ID+ EY  +MF
Sbjct:  168 LKPLLAQETLDEMDTNKDSVIDINEYLEDMF 198          
BLAST of SMED30009274 vs. Planmine SMEST
Match: SMESG000012033.1 (SMESG000012033.1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.351e-19
Identity = 66/159 (41.51%), Postives = 98/159 (61.64%), Query Frame = -1
Query:  624 RDFSDVEQHVDAEVHDVNYDHKAFLGKEEAEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWMLDERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDRELDWQKWQLADDNKDGLLDREEFFAFVRPEEG 1100
             + SD+E H D + HDVNYDH AFLGK+E+ KY  ++PEESK +L +++ +++D N DGK+   ELK WI   S++++ ++ D+ W DY  + K +L F  Y +  + D  E    AD +KR       D ++W  AD + D LL +EEFF F+ PEE 
Sbjct:   29 HELSDIEHHQDDDDHDVNYDHDAFLGKDESSKYKNMSPEESKEKLSKIV-DKIDVNSDGKIYPDELKHWITIQSQRYLREDTDKNWADYTIE-KDKLTFENYKKKAYEDDDE----ADMVKR-------DERRWAKADIDGDNLLTKEEFFDFLHPEES 174          
The following BLAST results are available for this feature:
BLAST of SMED30009274 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALU5.745e-2537.25calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU5.843e-2537.25calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU2.290e-2435.95calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU2.465e-2435.95calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU6.674e-2437.25calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
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BLAST of SMED30009274 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
calu-19.609e-2332.34CALUmenin (Calcium-binding protein) homolog [Sour... [more]
calu-19.609e-2332.34CALUmenin (Calcium-binding protein) homolog [Sour... [more]
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BLAST of SMED30009274 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scf1.253e-2636.71gene:FBgn0025682 transcript:FBtr0089942[more]
CG316503.210e-1031.90gene:FBgn0031673 transcript:FBtr0301907[more]
CG316503.210e-1031.90gene:FBgn0031673 transcript:FBtr0301905[more]
CG316503.210e-1031.90gene:FBgn0031673 transcript:FBtr0079033[more]
CG316503.210e-1031.90gene:FBgn0031673 transcript:FBtr0079034[more]
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BLAST of SMED30009274 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calua6.951e-2740.14calumenin a [Source:NCBI gene;Acc:415241][more]
calua6.951e-2740.14calumenin a [Source:NCBI gene;Acc:415241][more]
rcn32.004e-2434.84reticulocalbin 3, EF-hand calcium binding domain [... [more]
rcn32.004e-2434.84reticulocalbin 3, EF-hand calcium binding domain [... [more]
rcn32.037e-2434.84reticulocalbin 3, EF-hand calcium binding domain [... [more]
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BLAST of SMED30009274 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calu3.667e-2433.33calumenin [Source:NCBI gene;Acc:407965][more]
calu4.252e-2232.16calumenin [Source:NCBI gene;Acc:407965][more]
rcn36.324e-2035.76reticulocalbin 3 [Source:NCBI gene;Acc:395023][more]
fgf111.811e-1938.73fibroblast growth factor 11 [Source:Xenbase;Acc:XB... [more]
rcn38.318e-1938.62reticulocalbin 3 [Source:NCBI gene;Acc:395023][more]
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BLAST of SMED30009274 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Calu2.345e-2435.29calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Calu4.917e-2433.92calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Calu1.759e-2333.92calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Rcn19.892e-2034.97reticulocalbin 1 [Source:MGI Symbol;Acc:MGI:104559... [more]
Rcn31.132e-1730.00reticulocalbin 3, EF-hand calcium binding domain [... [more]
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BLAST of SMED30009274 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6IQP3|CALUA_DANRE4.232e-2640.14Calumenin-A OS=Danio rerio OX=7955 GN=calua PE=2 S... [more]
sp|Q6XLQ7|CALU_RABIT7.493e-2535.09Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU... [more]
sp|O43852|CALU_HUMAN2.534e-2437.25Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=... [more]
sp|Q5RDD8|CALU_PONAB3.379e-2437.25Calumenin OS=Pongo abelii OX=9601 GN=CALU PE=2 SV=... [more]
sp|B5X186|CALUA_SALSA2.188e-2337.50Calumenin-A OS=Salmo salar OX=8030 GN=calua PE=2 S... [more]
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BLAST of SMED30009274 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1B4XSS51.932e-8679.33Calumenin OS=Dugesia japonica OX=6161 GN=Djcalu PE... [more]
A0A3B4DL501.437e-2640.52Calumenin a OS=Pygocentrus nattereri OX=42514 PE=4... [more]
A0A0A9WL964.419e-2641.98Calumenin-A OS=Lygus hesperus OX=30085 GN=calua PE... [more]
A0A4Y2BL244.686e-2641.76Calumenin OS=Araneus ventricosus OX=182803 GN=Calu... [more]
A0A087UJQ46.319e-2640.37Calumenin-A (Fragment) OS=Stegodyphus mimosarum OX... [more]
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BLAST of SMED30009274 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calua1.136e-2739.22calumenin [Source:NCBI gene;Acc:103043492][more]
rcn33.781e-2736.65reticulocalbin 3 [Source:NCBI gene;Acc:103045651][more]
calub2.089e-2238.03calumenin-B [Source:NCBI gene;Acc:103027690][more]
rcn11.962e-2034.25reticulocalbin 1 [Source:NCBI gene;Acc:103039743][more]
rcn29.562e-723.33reticulocalbin 2 [Source:NCBI gene;Acc:103045530][more]
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BLAST of SMED30009274 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CALU1.914e-2235.17calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU1.623e-1531.86calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
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BLAST of SMED30009274 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009274 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO285543.042e-2341.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO358442.883e-2241.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO326387.108e-2134.34Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO476732.340e-2036.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427891.803e-1736.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30009274 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rcn39.655e-2838.71reticulocalbin-1 [Source:NCBI gene;Acc:101175408][more]
calub3.186e-2437.91calumenin [Source:NCBI gene;Acc:101165303][more]
rcn12.186e-2238.36reticulocalbin 1 [Source:NCBI gene;Acc:101175141][more]
pstpip1a1.297e-724.69proline-serine-threonine phosphatase-interacting p... [more]
pstpip1a3.992e-724.69proline-serine-threonine phosphatase-interacting p... [more]
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BLAST of SMED30009274 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000007225.11.869e-11199.44SMESG000007225.1[more]
SMESG000076911.14.726e-2739.52SMESG000076911.1[more]
SMESG000077404.14.923e-2136.49SMESG000077404.1[more]
SMESG000077404.15.760e-2136.49SMESG000077404.1[more]
SMESG000012033.11.351e-1941.51SMESG000012033.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009274 ID=SMED30009274|Name=SMED30009274|organism=Schmidtea mediterranea sexual|type=transcript|length=1268bp
CTTTTGTTTCTTTACCTACCGGACAATAATACTTTGCAGAATTCTCGAGT
AGTTTCGATGTAATTATTTTAATGCTTTTATTCCAAAGGTCATTTGTTGT
AGTCTAATTGTTGGTGAATCTATGAATTTTTTGTCGTTGGTTTCGTATTC
TTTCGACCAATTTTATAATAAAATACTGACCAATTAAAATATTAACCAAT
TAAATACTAGACTATAATTTTAAGCACCGAATTTCAGAAAAATTAACTTT
TATATTGAAAATTAGACTTAACCGTAAGAACTAATTATTTGTATAATAAT
TGGTTCTTTTCAAAAATTACGCTAAATACGAATCGATAAATTTTACACAA
TTGTTTGGTTTTATGTAAATTGTATTTAAAAAAAGAAATATTTATATTGA
TTCTTAACTAAGGTTATTTTTTAATTATTTATTTTTTATTTTTCCTACCC
CCTTCAATACAGCTCTGATTAAGATCGGCTACAGAACAAGTCAGAGAACA
AATCGGCATGCCATTGCACAATCAAACATATTACCGGTATATTCCTCTAG
GTCTATGTAACCATCATGATTTTTATCTGCTCTATCTAACATTTCATCGA
TCAAAAATGGTCGCATTCTAGGCACCTTCTTCTGGTCGAACAAATGCAAA
AAATTCTTCTCGATCTAGCAATCCATCTTTATTGTCATCCGCCAATTGCC
ACTTTTGCCAATCTAATTCTCTGTCTTTTAATACACGCTTCAATTCATCC
GCTGGATATCGTTCCTCAATTTTATCTCCAAAATTAATTTCAGAGTATCC
ATTGAAATCTAGCTGGGTTTTGTTTTTGGGGTTATAATCATTCCACACTC
TGTCTCTTTCATCTAACATCCACTTTTTGGAAATAAATTTAATCCAATCC
TTTAATTCTGATTTAGTAACTTTACCATCTTTATTAGTATCAACTCTATT
TTCTATTAATCTTCTAAGTCTTATCTTGCTTTCCTCAGGTGTCAATTCAG
AATATTTCTCAGCTTCTTCTTTGCCCAAAAAGGCTTTATGATCATAATTC
ACATCGTGAACTTCAGCATCGACATGCTGTTCCACATCACTGAAATCTCG
TTGTCTTGTACTTCCAGATAAAACTGCGACCAAAAAGCATAAAAATAACA
ATAATTTGATCATGATTGTTACACTACTATTGCCACTAGAAAAATGGAAA
TTTTCCAGAAAAGTGAAACGTCACAATTACACGTCACGATTACACGTCAC
CAATTTTGACCTTGGGAT
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protein sequence of SMED30009274-orf-1

>SMED30009274-orf-1 ID=SMED30009274-orf-1|Name=SMED30009274-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=182bp
MIKLLLFLCFLVAVLSGSTRQRDFSDVEQHVDAEVHDVNYDHKAFLGKEE
AEKYSELTPEESKIRLRRLIENRVDTNKDGKVTKSELKDWIKFISKKWML
DERDRVWNDYNPKNKTQLDFNGYSEINFGDKIEERYPADELKRVLKDREL
DWQKWQLADDNKDGLLDREEFFAFVRPEEGA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000019muscle progenitor cell
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000428musculature system
PLANA:0000478pharynx musculature
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 59..79
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 28..181
e-value: 2.8E-9
score: 39.3
NoneNo IPR availablePANTHERPTHR10827:SF52IP16409Pcoord: 6..180
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 6..180
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.867
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 156..171
e-value: 0.015
score: 14.8
coord: 73..88
e-value: 2.2E-4
score: 20.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 61..97
score: 10.636
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 146..181
score: 9.213
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 159..171
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 75..87
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 60..176