Collagen alpha-6(VI) chain
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009269 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: COL6A6 (collagen type VI alpha 6 chain [Source:HGNC Symbol;Acc:HGNC:27023]) HSP 1 Score: 121.709 bits (304), Expect = 1.609e-28 Identity = 100/393 (25.45%), Postives = 196/393 (49.87%), Query Frame = 1
Query: 199 ITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNT-ENRIAF--IKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDS----KLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
I S C I + DV+F++D S SI D++ + K F M+ ++K ++ +N++ F +K+ DD E++F + +++ + N + + ST +AL + ++ R K V +++++ TDG ++ A L++ + +L +GI + L S VE+F GL S V + +C + C +++ DLV ++D S SI D+++ K FL + +F++ + R+ FSD +F + ++ +++++ + G T IG ALREV+ + S+ P +L++TDG + + A L+ +G +I S+GIG+ + + +++ + +K LTV + +LKK K +++ IC
Sbjct: 795 ICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDF---MIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFS-DHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCN----SSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQ--DEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRN-IC 1176 HSP 2 Score: 114.39 bits (285), Expect = 4.465e-26 Identity = 91/362 (25.14%), Postives = 174/362 (48.07%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVY-ADKLKEANVQILTIGIGSDININELTRLASPGLSLQ-VESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE-DKILTVETYGDLKK 1305
D+ ++DGS S DF K F +++ + + + R+ +++ D ++ FE Y+ + + + N R + +T G ALN TL + ++ K+ TN M + A++L+E ++++ IGI + N +L +A + V F L ++QVV IC E+ C + D++ ++DSS SI E++ + K F+ +S ++GP++ ++ ++ FSD + +F + D+ N + + G T G AL V + RPN+ +++ITDG ++ P A L+ +G I S+G+ N+ +L+ IS + + VE + L++
Sbjct: 436 DIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGI-KEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEIC------TEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEP---AVVLRQEGVIIYSVGVFGS-NVTQLEEISGRPEMVFYVENFDILQR 786 HSP 3 Score: 107.071 bits (266), Expect = 1.024e-23 Identity = 98/382 (25.65%), Postives = 184/382 (48.17%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT--MRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGI-GSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
AC + D++F++D S SI ++F K F K++++ + +I ++F D + F+ + + + ++ +T G AL+ + PT R + K ++L TDG ++ P V L++ V I ++G+ GS N+ +L ++ P + VE+F L + + +V IC P E+ +E D+V ++DSS SI ++Y K+F++ + ++G + R L ++D+ F D+ ++I+ +++ GG+T +AL D S+ VP +++ITDG ++ + A L+ KG ++++GI ++ L M S DK VET+G LK V +V
Sbjct: 615 ACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVV---LRQEGVIIYSVGVFGS--NVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGIC---SP-REECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVC 987 HSP 4 Score: 94.7449 bits (234), Expect = 1.139e-19 Identity = 94/390 (24.10%), Postives = 180/390 (46.15%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM---RKE--VEKLVVLFT--DGTNNMYPAPYVYADKLKEANVQILTIGI--GSDININELTRLASPGLSLQVESFTGL-------LKTQSQVVSHICPI---PDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKL--ISQQRPN-VPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
DV+F+LD S + S+++F+ K F ++ ++ + R+ + + ++ +++ ++L + N G A+ KEV+ RK V ++ VL T D +N+ A A L+ V I T+GI SD + ++ + ++++F L LK ++H + L+ C E+ D+ +++D S S D+ + K FL + + F + P K RV + ++D +F Y D+ ++++ + GG T G AL S L +QR N VP +++++T+G++ LE ANRL+ + + ++GI +E N +L+ I+ E+K + D K ++ + IC
Sbjct: 229 DVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKA----AVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSK--DSILEPANRLREEHIRVYAIGI-KEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEIC 611 HSP 5 Score: 59.6918 bits (143), Expect = 1.195e-8 Identity = 52/203 (25.62%), Postives = 92/203 (45.32%), Query Frame = 1
Query: 745 VVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS---QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
+ SHI D G E D+V ++DSS+ + + + K F+ K +S+ + +K RVA+ +SD++ +F + + M+N ++ F GG+ IGKAL+E S R F +++ + E + L+ G IIS+G+ E NLK + + TV +++ ++K VI
Sbjct: 11 ICSHISVNQDSGPE------YADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVI 207
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: COL6A5 (collagen type VI alpha 5 chain [Source:HGNC Symbol;Acc:HGNC:26674]) HSP 1 Score: 108.227 bits (269), Expect = 4.839e-24 Identity = 93/363 (25.62%), Postives = 174/363 (47.93%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKL---VVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQV-ESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK-ILTVETYGDLK 1302
D+ F++DGS SI + FE K+F ++ + + R+ +++ DD E+ F Y+ + + N + L T GKAL+ L+ + M+ + K+ +++ TDG + + P A +L+ + + +GIG+ I EL +A + ++F L +++VV IC EK C + D++ ++DSS SI E++ + K F+ L+ ++G +K ++ ++ FSD+ + +F Y+ ++ + + + T GKAL V + R +++ITDGV R + A L+ K I S+G+ N +L+ IS + + VE + LK
Sbjct: 442 DIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEP---AKRLRAEQITVHAVGIGAANKI-ELQEIAGKEERVSFGQNFDALKSIKNEVVREICA------EKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVR--DPARILRGKDVTIFSVGV-YNANRSQLEEISGDSSLVFHVENFDHLK 791 HSP 2 Score: 100.908 bits (250), Expect = 1.205e-21 Identity = 94/389 (24.16%), Postives = 185/389 (47.56%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT--MRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ----RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELK-MISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQV 1356
C + D++F++D S SI ++F K F K+++T I+ + +I ++F D + F+ Y +++ + + T GKALN + + R +K ++L TDG + P A L+ +V I ++G+ + N ++L ++ L VE+F L + +++ +C + D K TL D+V +LD S SI ++ + N + + ++G ++ + L +SD+ F Y +I ++ GG T KAL+ ++ + + NV +++ITDG ++ + + A L+ KG I ++G+G + N KEL+ M +++ + V+ + LK T+++ +C P+V
Sbjct: 621 GCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDP---ARILRGKDVTIFSVGV-YNANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDC---KRITL--LDVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGVG-KANQKELEGMAGNKNNTIYVDNFDKLKDVF-TLVQERMCTEAPEV 998 HSP 3 Score: 80.4925 bits (197), Expect = 3.447e-15 Identity = 85/355 (23.94%), Postives = 167/355 (47.04%), Query Frame = 1
Query: 319 MTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE-VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGL--------LKTQSQVVSHICPIPDP-GLEKPCTLE--QQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALR---EVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
+ ++ SN+ I+F+K + EFQ K + + +NT I + E Y + R + V ++ VL T ++ +L++ NV L+I ++ + E+ ++S+ L K Q+++ S I + L+K ++ + D+ ++D S+SI E+ +EQ K F+++ F +GP+K RV ++ +SD+ +F TDY D+ + ++ + G T GKAL ++ + + VP ++++TDG++ R +E A RL+ + + ++GIG N EL+ I+ +++ ++ D KS+K + IC
Sbjct: 274 LGLMSYSNSAKTISFLK---SSTTQSEFQQQIKNLSIQVGKSNT-----GAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVHDAALNLRLEDVNVFALSIQGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMST--DRVVEPAKRLRAEQITVHAVGIG-AANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREIC 617 HSP 4 Score: 56.9954 bits (136), Expect = 7.511e-8 Identity = 45/182 (24.73%), Postives = 85/182 (46.70%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS-----QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTV 1332
D+V ++DSS+ + + + K F+ K +++ + K RVA+ +SDE +F + + M+N +K +F GG+ IGKAL+E S + R P ++++ + E + L+ G IIS+G+ E NLK + + T+ +++ ++K V
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESE--DEVEEASKALQKDGVKIISVGVQKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDV 209
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: COL6A5 (collagen type VI alpha 5 chain [Source:HGNC Symbol;Acc:HGNC:26674]) HSP 1 Score: 108.227 bits (269), Expect = 4.928e-24 Identity = 93/363 (25.62%), Postives = 174/363 (47.93%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKL---VVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQV-ESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK-ILTVETYGDLK 1302
D+ F++DGS SI + FE K+F ++ + + R+ +++ DD E+ F Y+ + + N + L T GKAL+ L+ + M+ + K+ +++ TDG + + P A +L+ + + +GIG+ I EL +A + ++F L +++VV IC EK C + D++ ++DSS SI E++ + K F+ L+ ++G +K ++ ++ FSD+ + +F Y+ ++ + + + T GKAL V + R +++ITDGV R + A L+ K I S+G+ N +L+ IS + + VE + LK
Sbjct: 442 DIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEP---AKRLRAEQITVHAVGIGAANKI-ELQEIAGKEERVSFGQNFDALKSIKNEVVREICA------EKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVR--DPARILRGKDVTIFSVGV-YNANRSQLEEISGDSSLVFHVENFDHLK 791 HSP 2 Score: 100.523 bits (249), Expect = 1.307e-21 Identity = 94/389 (24.16%), Postives = 185/389 (47.56%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT--MRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ----RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELK-MISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQV 1356
C + D++F++D S SI ++F K F K+++T I+ + +I ++F D + F+ Y +++ + + T GKALN + + R +K ++L TDG + P A L+ +V I ++G+ + N ++L ++ L VE+F L + +++ +C + D K TL D+V +LD S SI ++ + N + + ++G ++ + L +SD+ F Y +I ++ GG T KAL+ ++ + + NV +++ITDG ++ + + A L+ KG I ++G+G + N KEL+ M +++ + V+ + LK T+++ +C P+V
Sbjct: 621 GCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDP---ARILRGKDVTIFSVGV-YNANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDC---KRITL--LDVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGVG-KANQKELEGMAGNKNNTIYVDNFDKLKDVF-TLVQERMCTEAPEV 998 HSP 3 Score: 80.4925 bits (197), Expect = 3.698e-15 Identity = 85/358 (23.74%), Postives = 168/358 (46.93%), Query Frame = 1
Query: 319 MTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE-VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGL--------LKTQSQVVSHICPIPDP-GLEKPCTLE--QQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALR---EVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
+ ++ SN+ I+F+K + EFQ K + + +NT I + E Y + R + V ++ VL T ++ +L++ NV L+I ++ + E+ ++S+ L K Q+++ S I + L+K ++ + D+ ++D S+SI E+ +EQ K F+++ F +GP+K RV ++ +SD+ +F TDY D+ + ++ + G T GKAL ++ + + VP ++++TDG++ R +E A RL+ + + ++GIG N EL+ I+ +++ ++ D KS+K + IC +
Sbjct: 274 LGLMSYSNSAKTISFLK---SSTTQSEFQQQIKNLSIQVGKSNT-----GAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVHDAALNLRLEDVNVFALSIQGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMST--DRVVEPAKRLRAEQITVHAVGIG-AANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEK 620 HSP 4 Score: 56.9954 bits (136), Expect = 7.642e-8 Identity = 45/182 (24.73%), Postives = 85/182 (46.70%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS-----QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTV 1332
D+V ++DSS+ + + + K F+ K +++ + K RVA+ +SDE +F + + M+N +K +F GG+ IGKAL+E S + R P ++++ + E + L+ G IIS+G+ E NLK + + T+ +++ ++K V
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESE--DEVEEASKALQKDGVKIISVGVQKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDV 209
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: VIT (vitrin [Source:HGNC Symbol;Acc:HGNC:12697]) HSP 1 Score: 102.064 bits (253), Expect = 2.616e-22 Identity = 95/391 (24.30%), Postives = 171/391 (43.73%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTK---LKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM---RKEVEKLVVLFTDGTNNMYPAPYVY-ADKL-KEANVQILTIGIGSDININELTRLASPGL------------SLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLK-ELKMISSE---DKILTVETYGDLKKSLKTVMKTVIC 1338
D+ F++DGS SI K F + K+ D+ + + +++GD+ F +T+T LK + +T L + +G+A++ K + R +VV+ DG +P V A +L +E+ + I I I NE + P SL V+S+ GL KT +V +C K C L D+ ++D S+S+ ++ F+ FE+ R+ + ++ E R +FGF Y D++N +K + + G T G A+ +L + +PN +++ITDG + R A LK +I+ IG + EL++I++ D V+ + +L + + +++ IC
Sbjct: 272 DLSFLIDGSTSIGKRRFRIQKQLLADVAQALDIGPAGPLMGVVQYGDNPATHFNLKTHTNSRDLKTAIEKITQRGGL---SNVGRAISFVTKNFFSKANGNRSGAPNVVVVMVDG----WPTDKVEEASRLARESGINIFFITIEGAAE-NEKQYVVEPNFANKAVCRTNGFYSLHVQSWFGLHKTLQPLVKRVCDTDRLACSKTC-LNSADIGFVIDGSSSVGTGNFRTVLQFVTNLTKEFEISDTDTRIGAVQYTYEQRLEFGFDKYSSKPDILNAIKRVGYWSGGTSTGAAINFALEQLFKKSKPNKRKLMILITDGRSYDDVRIPAMAAHLK----GVITYAIGVAWAAQEELEVIATHPARDHSFFVDEFDNLHQYVPRIIQN-IC 648
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: VIT (vitrin [Source:HGNC Symbol;Acc:HGNC:12697]) HSP 1 Score: 101.679 bits (252), Expect = 3.081e-22 Identity = 91/388 (23.45%), Postives = 168/388 (43.30%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM---RKEVEKLVVLFTDGTNNMYPAPYVY-ADKL-KEANVQILTIGIGSDININELTRLASPGL------------SLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLK-ELKMISSE---DKILTVETYGDLKKSLKTVMKTVIC 1338
D+ F++DGS SI K F + K+ D+ + + +++GD+ F +T+T + + + + +G+A++ K + R +VV+ DG +P V A +L +E+ + I I I NE + P SL V+S+ GL KT +V +C K C L D+ ++D S+S+ ++ F+ FE+ R+ + ++ E R +FGF Y D++N +K + + G T G A+ +L + +PN +++ITDG + R A LK +I+ IG + EL++I++ D V+ + +L + + +++ IC
Sbjct: 245 DLSFLIDGSTSIGKRRFRIQKQLLADVAQALDIGPAGPLMGVVQYGDNPATHFNLKTHTNSRDLKTAIEKITQRGGLSNVGRAISFVTKNFFSKANGNRSGAPNVVVVMVDG----WPTDKVEEASRLARESGINIFFITIEGAAE-NEKQYVVEPNFANKAVCRTNGFYSLHVQSWFGLHKTLQPLVKRVCDTDRLACSKTC-LNSADIGFVIDGSSSVGTGNFRTVLQFVTNLTKEFEISDTDTRIGAVQYTYEQRLEFGFDKYSSKPDILNAIKRVGYWSGGTSTGAAINFALEQLFKKSKPNKRKLMILITDGRSYDDVRIPAMAAHLK----GVITYAIGVAWAAQEELEVIATHPARDHSFFVDEFDNLHQYVPRIIQN-IC 621
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: mup-4 (Transmembrane matrix receptor MUP-4 [Source:UniProtKB/Swiss-Prot;Acc:Q21281]) HSP 1 Score: 86.6557 bits (213), Expect = 1.276e-17 Identity = 55/197 (27.92%), Postives = 102/197 (51.78%), Query Frame = 1
Query: 781 LEKPCTLEQQDLVVILDSSNSISEEDYE-QGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFN-----ILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
L C + DLV ++D S SI ++ + FL +F F++ P+K RV+++ +SD+IR +FG +Y + N ++++ G T G A+ V ++ S++R P ++ITDG + RP + A R ++ + ++G+ + EL+ IS S+D+ V + DL L++ ++ V C +Q
Sbjct: 427 LSTACPAQPTDLVFLIDGSGSIGSYVFQTEVLRFLAEFTELFDIAPQKTRVSVVQYSDQIRHEFGLDNYSDRKSLQNAIRNIEYLTGLTRTGAAIEHVANEAFSERRGARPVGQVSRVAIVITDGRSQDNVTRPSDNARRQDIQ---LFAVGVTNHVLDAELEEISGSKDRTFHVSGFEDLNTRLRSAIQRVACPHQ 620 HSP 2 Score: 50.447 bits (119), Expect = 3.263e-6 Identity = 41/199 (20.60%), Postives = 87/199 (43.72%), Query Frame = 1
Query: 196 QITSLTAACTFIIRDVLFILDGSQSISKDDFEL-AKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLA-SPGLSLQVESFTGL-LKTQSQVVSHICP 765
++ +L+ AC D++F++DGS SI F+ +F + + + + R++ +++ D F Y+ K + + N L T G A+ E + R +V ++ ++ TDG + + P +D + ++Q+ +G+ + + EL ++ S + V F L + +S + CP
Sbjct: 423 RVCTLSTACPAQPTDLVFLIDGSGSIGSYVFQTEVLRFLAEFTELFDIAPQKTRVSVVQYSDQIRHEFGLDNYSDRKSLQNAIRNIEYLTGLTRTGAAIEHVANEAFSERRGARPVGQVSRVAIVITDGRSQDNVTRP---SDNARRQDIQLFAVGVTNHVLDAELEEISGSKDRTFHVSGFEDLNTRLRSAIQRVACP 618
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: mua-3 (Transmembrane cell adhesion receptor mua-3 [Source:UniProtKB/Swiss-Prot;Acc:P34576]) HSP 1 Score: 71.2478 bits (173), Expect = 1.042e-12 Identity = 49/202 (24.26%), Postives = 100/202 (49.50%), Query Frame = 1
Query: 781 LEKPCTLEQQDLVVILDSSNSISEEDYE-QGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFN------ILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETYGDLKKSLKTVMKTVICGNQPQVE 1359
++ C ++ DLV ++D S SI ++ + F+ +F+ FE+G K RV ++ +SD+IR +F Y D ++ + G T G A++ + + S++R P +I+TDG + P + A +L + N ++G+ + + EL+ I+ S ++ V+ + DL L+++++ C + + E
Sbjct: 636 VQTTCPKQKTDLVFLIDGSGSIGSYVFKNEVLRFVREFVELFEIGRSKTRVGLIQYSDQIRHEFDLDQYGDRDSLLKGISETQYLTGLTRTGAAIQHMVQEGFSERRGARPQQSDIARVAIILTDGRSQDNVTGPADSARKLSI---NTFAIGVTDHVLASELESIAGSPNRWFYVDKFKDLDTRLRSMIQKAACPSPTKQE 834
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: mua-3 (Transmembrane cell adhesion receptor mua-3 [Source:UniProtKB/Swiss-Prot;Acc:P34576]) HSP 1 Score: 71.2478 bits (173), Expect = 1.057e-12 Identity = 49/202 (24.26%), Postives = 100/202 (49.50%), Query Frame = 1
Query: 781 LEKPCTLEQQDLVVILDSSNSISEEDYE-QGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFN------ILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETYGDLKKSLKTVMKTVICGNQPQVE 1359
++ C ++ DLV ++D S SI ++ + F+ +F+ FE+G K RV ++ +SD+IR +F Y D ++ + G T G A++ + + S++R P +I+TDG + P + A +L + N ++G+ + + EL+ I+ S ++ V+ + DL L+++++ C + + E
Sbjct: 1220 VQTTCPKQKTDLVFLIDGSGSIGSYVFKNEVLRFVREFVELFEIGRSKTRVGLIQYSDQIRHEFDLDQYGDRDSLLKGISETQYLTGLTRTGAAIQHMVQEGFSERRGARPQQSDIARVAIILTDGRSQDNVTGPADSARKLSI---NTFAIGVTDHVLASELESIAGSPNRWFYVDKFKDLDTRLRSMIQKAACPSPTKQE 1418
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: cutl-23 (CUTiclin-Like [Source:UniProtKB/TrEMBL;Acc:A0A168H2V5]) HSP 1 Score: 62.3882 bits (150), Expect = 4.835e-10 Identity = 47/187 (25.13%), Postives = 89/187 (47.59%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKA----LREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYAN--RLKMKGANIISLGIGEEINLKELK-MISSEDKILTVETYGDLKKSLKTVMKTVICGNQP 1350
DLV ILDSS S+ ++ ++ + + + L + +G RV ++ FS +F F + ++++ + L G T IG A L+++ + + VP + +++DG + FP+ + A+ R ++ +I + G G+ + + L SE KI+T L+ + T IC QP
Sbjct: 54 DLVFILDSSGSLRDK-FQDEIDIIRRILKHVTIGKTATRVMLIQFSGTQHLEFNFEKFTDREELLAALDVLRHVSGITRIGGAFEFALQQLKTPGSGLRDGTVPKIVYLLSDGRTHDFPKDWQMADILRREIPNIDIWAYGTGDYVAMAALANYTQSEQKIVTNLNLQKLEPQFEKYHGTEICEQQP 239
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: cut-6 (CUTiclin [Source:UniProtKB/TrEMBL;Acc:Q21540]) HSP 1 Score: 62.003 bits (149), Expect = 5.469e-10 Identity = 36/177 (20.34%), Postives = 90/177 (50.85%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKEL-KMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
+++++LD+S SI ++ +++ +F + S + + + +A++ +++ + +F + + ++ + GAT G+ALR K + RP +P ++ITDG + E + L+ + ++G+ +N+ +L +M + ++ TVE++ L ++L + +C
Sbjct: 47 EVILLLDASGSIGDDTFKKQLSFAMHLASRLNISEDGSHMALIQYAETPKLEFSLGQFNHPTQLEWAIQRIEYQSGATNTGQALRLTLEKGLQGARPGIPKVAIVITDGQSQ--DDVSEPSQLLRDADVMVYAIGVTNLVNVHQLHQMTGNPVRVFTVESFEQLDRALADSLTWSMC 221 HSP 2 Score: 59.3066 bits (142), Expect = 3.936e-9 Identity = 40/165 (24.24%), Postives = 81/165 (49.09%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRL-ASPGLSLQVESFTGL 726
+V+ +LD S SI D F+ F + + + S + +A I++ + ++ F + Q+ + +T G+AL LTL++ R + K+ ++ TDG + + P + L++A+V + IG+ + +N+++L ++ +P VESF L
Sbjct: 47 EVILLLDASGSIGDDTFKKQLSFAMHLASRLNISEDGSHMALIQYAETPKLEFSLGQFNHPTQLEWAIQRIEYQSGATNTGQALRLTLEKGLQGARPGIPKVAIVITDGQSQDDVSEP---SQLLRDADVMVYAIGVTNLVNVHQLHQMTGNPVRVFTVESFEQL 208
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303]) HSP 1 Score: 102.064 bits (253), Expect = 2.471e-22 Identity = 95/370 (25.68%), Postives = 175/370 (47.30%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFG----DDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT------MRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIG--SDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETY 1290
+ DV+F++ ++ I DFE K F +++++ ++ + + I+FG D F Y +Q+ +T+ ++ S +AL +L Y T ++ V +++ + TDG AD+ + + IG+ D + +T+ S V ++ L Q V +C P C E DLV ++D S SISEE + FL+ + +GPE RV + FS + +F +Y +D + + ++ + + T IGKAL V S+++ VP N+++ITDGV++ R E A++L+ G N+ ++GIG ++L++L I+ S D++ V+ +
Sbjct: 993 LADVIFLVQCTRQIRLQDFEKIKSF---LISVVNSTQIGDNL--IRFGVIVYSDTPSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSL--TYFTEANGGRQKRGVPQMLFMITDGDARDRENLRARADEFAAKQINVYGIGVARAQDSELEIITK--SKNKIFHVNNYDNLQDLQMNVSGVLCNATKP----VCQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFSSVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVEFFQEDKGSRKQSGVPQNLVLITDGVSS--DRVNEAADQLRNLGINVFAIGIG-AVSLQQLSYIAGSPDRLFKVQNF 1346 HSP 2 Score: 87.0409 bits (214), Expect = 1.884e-17 Identity = 92/410 (22.44%), Postives = 185/410 (45.12%), Query Frame = 1
Query: 187 QNDQITSLTAAC-TFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE-----VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS---PGLSLQVESFTGLLKTQSQVVSHICPIP-----DPG----LEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNI----LIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
Q ++ ++T C + + D++FI+D S SI++++FEL K+F +++ ++ ++ R+ + + D F T+ ++ + T G AL ++ R V+++ ++ TDG + A +L+ + V + +G+ + ++ EL ++ S V SF L + + +C + D L++ C E+ D+ +LD+S S + D+E K F++ L F +GP + RV ++ F T++ V+ + + G + GKAL+ V S L +Q + + P + ++ITD + + A L+++G ++ ++G+ + + LKM + E K V Y D LK + T IC +
Sbjct: 398 QEKEVFNVTGDCRSAQVADIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFANKNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDGESE--DDVTTTAAELRRSGVTVYALGV-KNASVEELKKIGSYPEHEFVFNVGSFLMLSSLEKSLRKSLCKVVVNRRFDKNNRLLLKQGCVNTEEADIYFLLDNSGS-TRADFEDVKKFILGSLQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGGSEKGKALKYVAS-LFNQAKASRPAKVQEILIVITDKTSQ--DDVGDPAEELRIQGVSVYAIGVKDASQDELLKMTADETKDFYVTNY-DALNVLKREIVTDICSQE 799 HSP 3 Score: 80.4925 bits (197), Expect = 1.819e-15 Identity = 92/388 (23.71%), Postives = 173/388 (44.59%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMM--TIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK--EVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVES-FTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSL----IKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG----VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETYGDLKKSLKTVMKTVIC 1338
AC + D++F++DGS SI DF K F ++ TII + + ++F ++ + F Y ++ + L T GKAL+ K + R +V + +V+ TDG + A V A +++ V I +IG+ S +N +L ++ + V+ F L + +C G P T + D++ ++ + I +D+E+ K+FL+ +++ ++G R ++ +SD F Y + + SL + A + +L +Q+ VP + +ITDG N+ R E+A K N+ +G+ + EL++I+ S++KI V Y +L + L+ + V+C
Sbjct: 800 ACKNKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVH--VGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYTGKALSFISKYFDASNGGRPDVPQFLVVITDG--EAHDAVAVPAKAIRDKGVTIFSIGVAS-VNTTQLWEISGTQDKVYVQRDFDALHSIDKNLQFKLCSSHPGGC--PST-QLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVIVYSDTPS-QFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDGDARDRENLRARADEFA----AKQINVYGIGVARAQD-SELEIITKSKNKIFHVNNYDNL-QDLQMNVSGVLC 1172 HSP 4 Score: 72.4034 bits (176), Expect = 7.902e-13 Identity = 71/298 (23.83%), Postives = 141/298 (47.32%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT------MRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS---PGLSLQVESFTGLLKTQSQVVSHICPI-PDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDY---YKSDDMINKVKSLIKFGGATGIGKALREVDSKLIS 1089
D++F++D S SI DF K F + ++ + ++ ++F D F Y ++ + N T +GKA+N LK+ Y T + ++V ++VV+ TDG + + P A+ L++ + I IG+GS N+ EL +AS + ++++ L + + +C + D G K + D+ V++DS+ ++ + +Q + L + L +G N++A+ F + + +F F DY K+ + IN+ + + G +GKA+ V + ++
Sbjct: 35 DIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNF-LKDNYFTSAGGSRVSEKVPQVVVVITDGDSTDDIKGP---AEDLRKKGIIIFAIGVGS-TNMTELKAIASTPPERFVVNIDNYQALQGLTTTMKETVCVLMRDQG--KALAPKFTDVFVLVDST---AQGETQQIRELLNRLLVQLNVGDGSNKIALAQFGENVVKEFLFKDYQTAQKAREFINRFEP--RLNGERKLGKAIDYVRTNFLN 320 HSP 5 Score: 68.9366 bits (167), Expect = 9.550e-12 Identity = 54/185 (29.19%), Postives = 94/185 (50.81%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI-----SQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS---EDKILTVETYGDLKKSLKTVMKTVIC 1338
D+V ++DSS+SI D+ + K F+ F+ E+ +K +V ++ FS + +F +Y ++ K+ +L G T +GKA+ + S+ VP +++ITDG + P E L+ KG I ++G+G N+ ELK I+S E ++ ++ Y L + L T MK +C
Sbjct: 35 DIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDGDSTDDIKGPAE---DLRKKGIIIFAIGVG-STNMTELKAIASTPPERFVVNIDNYQAL-QGLTTTMKETVC 214 HSP 6 Score: 51.9878 bits (123), Expect = 1.649e-6 Identity = 49/200 (24.50%), Postives = 98/200 (49.00%), Query Frame = 1
Query: 220 CTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK--EVYPTMRKE--VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLA-SPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLE 804
C + D++F++DGS+SIS++ + F +++ ++ R+ +F + F Y + + + T IGKAL+ ++ + RK+ V + +VL TDG ++ AD+L+ + + IGIG+ +++ +L+ +A SP +V++F L T + V +C +P P + CT++
Sbjct: 1179 CQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFSSVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVEFFQEDKGSRKQSGVPQNLVLITDGVSSDRVNE--AADQLRNLGINVFAIGIGA-VSLQQLSYIAGSPDRLFKVQNFNYLNLTTATFVDALC-VPLPLAQASCTID 1374
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303]) HSP 1 Score: 101.293 bits (251), Expect = 4.670e-22 Identity = 95/370 (25.68%), Postives = 175/370 (47.30%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFG----DDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT------MRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIG--SDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETY 1290
+ DV+F++ ++ I DFE K F +++++ ++ + + I+FG D F Y +Q+ +T+ ++ S +AL +L Y T ++ V +++ + TDG AD+ + + IG+ D + +T+ S V ++ L Q V +C P C E DLV ++D S SISEE + FL+ + +GPE RV + FS + +F +Y +D + + ++ + + T IGKAL V S+++ VP N+++ITDGV++ R E A++L+ G N+ ++GIG ++L++L I+ S D++ V+ +
Sbjct: 968 LADVIFLVQCTRQIRLQDFEKIKSF---LISVVNSTQIGDNL--IRFGVIVYSDTPSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSL--TYFTEANGGRQKRGVPQMLFMITDGDARDRENLRARADEFAAKQINVYGIGVARAQDSELEIITK--SKNKIFHVNNYDNLQDLQMNVSGVLCNATKP----VCQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFSSVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVEFFQEDKGSRKQSGVPQNLVLITDGVSS--DRVNEAADQLRNLGINVFAIGIG-AVSLQQLSYIAGSPDRLFKVQNF 1321 HSP 2 Score: 85.8853 bits (211), Expect = 4.288e-17 Identity = 92/410 (22.44%), Postives = 185/410 (45.12%), Query Frame = 1
Query: 187 QNDQITSLTAAC-TFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE-----VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS---PGLSLQVESFTGLLKTQSQVVSHICPIP-----DPG----LEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNI----LIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
Q ++ ++T C + + D++FI+D S SI++++FEL K+F +++ ++ ++ R+ + + D F T+ ++ + T G AL ++ R V+++ ++ TDG + A +L+ + V + +G+ + ++ EL ++ S V SF L + + +C + D L++ C E+ D+ +LD+S S + D+E K F++ L F +GP + RV ++ F T++ V+ + + G + GKAL+ V S L +Q + + P + ++ITD + + A L+++G ++ ++G+ + + LKM + E K V Y D LK + T IC +
Sbjct: 373 QEKEVFNVTGDCRSAQVADIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFANKNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDGESE--DDVTTTAAELRRSGVTVYALGV-KNASVEELKKIGSYPEHEFVFNVGSFLMLSSLEKSLRKSLCKVVVNRRFDKNNRLLLKQGCVNTEEADIYFLLDNSGS-TRADFEDVKKFILGSLQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGGSEKGKALKYVAS-LFNQAKASRPAKVQEILIVITDKTSQ--DDVGDPAEELRIQGVSVYAIGVKDASQDELLKMTADETKDFYVTNY-DALNVLKREIVTDICSQE 774 HSP 3 Score: 80.1073 bits (196), Expect = 3.074e-15 Identity = 92/388 (23.71%), Postives = 173/388 (44.59%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMM--TIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK--EVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVES-FTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSL----IKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG----VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETYGDLKKSLKTVMKTVIC 1338
AC + D++F++DGS SI DF K F ++ TII + + ++F ++ + F Y ++ + L T GKAL+ K + R +V + +V+ TDG + A V A +++ V I +IG+ S +N +L ++ + V+ F L + +C G P T + D++ ++ + I +D+E+ K+FL+ +++ ++G R ++ +SD F Y + + SL + A + +L +Q+ VP + +ITDG N+ R E+A K N+ +G+ + EL++I+ S++KI V Y +L + L+ + V+C
Sbjct: 775 ACKNKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVH--VGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYTGKALSFISKYFDASNGGRPDVPQFLVVITDG--EAHDAVAVPAKAIRDKGVTIFSIGVAS-VNTTQLWEISGTQDKVYVQRDFDALHSIDKNLQFKLCSSHPGGC--PST-QLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVIVYSDTPS-QFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDGDARDRENLRARADEFA----AKQINVYGIGVARAQD-SELEIITKSKNKIFHVNNYDNL-QDLQMNVSGVLC 1147 HSP 4 Score: 71.633 bits (174), Expect = 1.244e-12 Identity = 75/300 (25.00%), Postives = 141/300 (47.00%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT------MRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQV-----ESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDY---YKSDDMINKVKSLIKFGGATGIGKALREVDSKLIS 1089
D++F++D S SI DF K F + ++ + ++ ++F D F Y ++ + N T +GKA+N LK+ Y T + ++V ++VV+ TDG + + P A+ L++ + I IG+GS N+ EL +AS P V ++ GL T + V + + D G K + D+ V++DS+ ++ + +Q + L + L +G N++A+ F + + +F F DY K+ + IN+ + + G +GKA+ V + ++
Sbjct: 10 DIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNF-LKDNYFTSAGGSRVSEKVPQVVVVITDGDSTDDIKGP---AEDLRKKGIIIFAIGVGS-TNMTELKAIASTPPERFVVNIDNYQALQGLTTTMKETVCVL--MRDQG--KALAPKFTDVFVLVDST---AQGETQQIRELLNRLLVQLNVGDGSNKIALAQFGENVVKEFLFKDYQTAQKAREFINRFEP--RLNGERKLGKAIDYVRTNFLN 295 HSP 5 Score: 68.9366 bits (167), Expect = 1.041e-11 Identity = 54/188 (28.72%), Postives = 95/188 (50.53%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALR--------EVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS---EDKILTVETYGDLKKSLKTVMKTVIC 1338
D+V ++DSS+SI D+ + K F+ F+ E+ +K +V ++ FS + +F +Y ++ K+ +L G T +GKA+ +S++ P V +++ITDG + P E L+ KG I ++G+G N+ ELK I+S E ++ ++ Y L + L T MK +C
Sbjct: 10 DIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNFLKDNYFTSAGGSRVSEKVPQV---VVVITDGDSTDDIKGPAE---DLRKKGIIIFAIGVG-STNMTELKAIASTPPERFVVNIDNYQAL-QGLTTTMKETVC 189 HSP 6 Score: 51.6026 bits (122), Expect = 2.561e-6 Identity = 50/202 (24.75%), Postives = 100/202 (49.50%), Query Frame = 1
Query: 220 CTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK--EVYPTMRKE--VEKLVVLFTDG--TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLA-SPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLE 804
C + D++F++DGS+SIS++ + F +++ ++ R+ +F + F Y + + + T IGKAL+ ++ + RK+ V + +VL TDG ++ + A AD+L+ + + IGIG+ +++ +L+ +A SP +V++F L T + V +C +P P + CT++
Sbjct: 1154 CQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFSSVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVEFFQEDKGSRKQSGVPQNLVLITDGVSSDRVNEA----ADQLRNLGINVFAIGIGA-VSLQQLSYIAGSPDRLFKVQNFNYLNLTTATFVDALC-VPLPLAQASCTID 1349
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: itgae.1 (integrin, alpha E, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-131121-125]) HSP 1 Score: 95.9005 bits (237), Expect = 1.105e-20 Identity = 92/388 (23.71%), Postives = 172/388 (44.33%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVY---PTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILT---IGIGSDININ-----ELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRP---NVPFNILIITDGVNNMFPRPL---EYANRLKMKGANIISLGIGEEI-----NLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
++ F+LDGS SI DDF+ AK F ++M+ + + + A +++G LL V + +Y+ T A+N L +++ + K++++ +DG + P + L + ++ +T IG+G I N E+ ++A PG V S+ L S + I DPG E + +LD S SI ++D+++ K+F+ +SN A++ + IR + D + KVK + + T A+ V + + ++ N I++++DG + P+ E N+ +MKG S+G+G+ I +KE+ I+ K +V +YG L L ++ + +I
Sbjct: 158 EIAFVLDGSGSIQDDDFQKAKDFIYNVMSNVWKTCFDCNFAIVQYGSLIRTELSLLDNEDRVGSLLKVKQIKQIYNLTKTASAINHVLTDIFIPENGSKDNSAKIIIVLSDG--KILGDPMTLDEVLNKPQMKGVTRYSIGVGDGILKNLDATEEMMQIADPGKYYNVSSYRALNDIVSSLERGIIGTKDPGTE---------IAFVLDGSGSIQDDDFQKAKDFIYNVMSNVWKTCFDCNFAIVQYGSLIRTELLLLDNEDRAGSLLKVKQIKQIYNLTKTASAINHVLTDIFIPEKGSKNNTAKIIIVLSDG--EILEDPMTLDEVLNKPQMKGVTRYSIGVGDGILKKPNAVKEMMQIADPGKYYSVSSYGALNDILSSLEREII 532
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: vwa2 (von Willebrand factor A domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-100302-1]) HSP 1 Score: 96.2857 bits (238), Expect = 1.356e-20 Identity = 90/384 (23.44%), Postives = 178/384 (46.35%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKE------VYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGI---GSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLIS---QQRPNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGN 1344
DVLF+L+GS +++ + F K F K M + +S+T ++ ++G D +I + +++ V + T G AL + V+ ++ ++ ++VVL T GT + A +V + +I IG+ G IN +T +P ++ +S L ++ + IC + + G C + DLV +LD+S+ + +E++ ++F+ F++ + +V ++ + F Y ++ V GG +G AL V S+ ++ RP V ++++TDG + P A +++ G +I +G+G+ L++ SED ++TV +Y DLK S + V+ ++C +
Sbjct: 342 DVLFLLEGSSALTLEGFLRFKSFLKRFMQAVLSSDTPVKMGLAQYGSDVKIEARIGEHRDPVKLIQAVEGLQYRGGQTKTGNALRYITRHGFQSTPVFADVQDDLPRVVVLLT-GTPS---ADHVVEPAKYARDREIFIIGVAPEGMRAEINNIT--GNPQRTIMYQSPDRLSSKIPELRAKICSVDNQG----CLGQALDLVFVLDASSGVGKENFIHFQDFVRSTSVQFDINRDVAQVGLVVYGRRPVTVFDLDKYNSGSAVLRAVGDAAFLGGKASVGSALLHVLSQSLTVGKGARPGVNKAVVVLTDGTGVEDAAVP-----AQKIRDSGVSIFLIGVGDIQQELLLRISGSEDHMITVPSYDDLKYS-EDVLVQMVCSD 709
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: vwa2 (von Willebrand factor A domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-100302-1]) HSP 1 Score: 96.2857 bits (238), Expect = 1.356e-20 Identity = 90/384 (23.44%), Postives = 178/384 (46.35%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKE------VYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGI---GSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLIS---QQRPNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGN 1344
DVLF+L+GS +++ + F K F K M + +S+T ++ ++G D +I + +++ V + T G AL + V+ ++ ++ ++VVL T GT + A +V + +I IG+ G IN +T +P ++ +S L ++ + IC + + G C + DLV +LD+S+ + +E++ ++F+ F++ + +V ++ + F Y ++ V GG +G AL V S+ ++ RP V ++++TDG + P A +++ G +I +G+G+ L++ SED ++TV +Y DLK S + V+ ++C +
Sbjct: 342 DVLFLLEGSSALTLEGFLRFKSFLKRFMQAVLSSDTPVKMGLAQYGSDVKIEARIGEHRDPVKLIQAVEGLQYRGGQTKTGNALRYITRHGFQSTPVFADVQDDLPRVVVLLT-GTPS---ADHVVEPAKYARDREIFIIGVAPEGMRAEINNIT--GNPQRTIMYQSPDRLSSKIPELRAKICSVDNQG----CLGQALDLVFVLDASSGVGKENFIHFQDFVRSTSVQFDINRDVAQVGLVVYGRRPVTVFDLDKYNSGSAVLRAVGDAAFLGGKASVGSALLHVLSQSLTVGKGARPGVNKAVVVLTDGTGVEDAAVP-----AQKIRDSGVSIFLIGVGDIQQELLLRISGSEDHMITVPSYDDLKYS-EDVLVQMVCSD 709
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: ENSXETT00000014204.1 (collagen type VI alpha 5 chain [Source:NCBI gene;Acc:100486063]) HSP 1 Score: 118.627 bits (296), Expect = 1.606e-27 Identity = 101/385 (26.23%), Postives = 182/385 (47.27%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMM--TIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM--RKEVEKLVVLFTDGTNNMYPAPY--VYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKL--ISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK-ILTVETYGDLKKSLKTVMKTVIC--GNQP 1350
+ D++F+LD S SI++ +F L ++F + ++ +++ N + + +G + F TY+ +L V + + T KAL T + R + +++L TDG P + + LK+ + + +G+G + EL ++A P V+++ GL + +C P C+ EQ DLV ++D S SI+ ++ K F+ + + +F + + R+ + +S + +F +YY S DM ++ S+ + T GK LR V + R NVP ++++TDG++N E A L+ G I S+GIG N EL MI+ K + VET+ L S+K + +C +QP
Sbjct: 1211 VADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQ--FGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVP-QELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPA----CSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVN--EDAAALRSSGVKIFSIGIGLR-NSFELVMIAGSPKNVYEVETFQAL-DSIKRQIVAQVCEPSDQP 1584 HSP 2 Score: 107.842 bits (268), Expect = 5.677e-24 Identity = 91/396 (22.98%), Postives = 193/396 (48.74%), Query Frame = 1
Query: 187 QNDQITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM-----RKEVEKLVVLFTDGTN-NMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS--PGLSL-QVESFTGLLKTQSQVVSHIC----------PIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI--SQQRPN-VPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLK 1302
Q ++ + A C+ + D++F++D S SI +F+L + F +++ + S + R+ + + D+ + + T+ + ++L +T G AL+ K+++ + V+++ V+ T+G + + + P A KL+ + V++ +G +IN EL +AS P + VESF L + ++ +C P +++ C E+ D+ ++D S SI ED+E K F+++ +S F++G + R ++ +SD R +F +++ + + + + + GG T G+AL + + ++ RP+ VP ++++ITDG + R E A ++ G I ++G+ + + + S +K+ V + LK
Sbjct: 615 QFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGF-QNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQ--DRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLK 1004 HSP 3 Score: 94.3597 bits (233), Expect = 1.290e-19 Identity = 88/384 (22.92%), Postives = 177/384 (46.09%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE----VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGI-----GSDININELTR--LASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
D+ F++DGS SI +DFE KKF +++++ + R +++ D F + K + ++ L T G+AL ++K+++ K+ V + +V+ TDG + D++ EA +I GI G + E R S V +F L ++ + +C + C + D+V ++DSS SI+ +DYE K F+ + E+GP++ ++ ++ FS E + +F Y + D++ + ++ + + T +G+AL+ + + R N +++ITDG + P A ++ G I ++G+ + N + L++ ++++ +++ L K +M IC Q
Sbjct: 840 DIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALT-SMKQLFVNAAKDRPHKVPQSLVVITDGESQ---------DRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELC------TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFE-ICNPQ 1203 HSP 4 Score: 90.1225 bits (222), Expect = 2.622e-18 Identity = 90/388 (23.20%), Postives = 177/388 (45.62%), Query Frame = 1
Query: 118 MNLFLAVLPILIGFFTFNVKTDDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP-----TMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVES---FTGLLKTQSQVVSHICPIPDPG------LEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ-----RPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLG 1215
M + L ++G ++ NV Q T T A I D++F++DGS SI ++F K F ++ + +I I++ D+A F +Y+ + +L + N + T G +L L + + + V ++ V+ TDG + P + +K A + + IGI D ++EL +AS V S F L ++ +C + + + C T Q D+V++++S+ + + +E+ KNFL +SN ++G K R+ +++++DE +F Y +++ ++++ G T G+AL V++ +Q +V ++I+T+G ++ P A LK +G ++ +G
Sbjct: 1 MGFWKVFLACVLGSYSVNVSAS----QKTVCTEAS---IADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAI---AVKNAGIILYAIGI-KDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVG 374 HSP 5 Score: 83.1889 bits (204), Expect = 5.031e-16 Identity = 91/394 (23.10%), Postives = 177/394 (44.92%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM--RKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQV---ESFTGLLKTQSQVVSHICPIPDPGLEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQ--QRPNVPFNILIITDGVNNMFPRP-LEYANR-LKMKGANIISLGIGEEINLKELKMISS-EDKILTVETYGDLKKSLKTVMKTVICGNQPQVEY 1362
AC + D++F++D S SI+ DD+E K+F + M+ + +I I+F + + F Y + ++ + + L T +G+AL TL + R ++ +++ TDG + P A +++ V I IG+ N +L +A G QV +SF L ++ IC +P ++ C E D++ +LD+S SI+ ++ + F+ +++ +G + + + + F Y D++ V SL + G T KAL + + RP + ++++TDG RP L ++ LK G + ++G+G+ + +EL+ I+ D+ V+ Y L + + V ++P +
Sbjct: 1019 ACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGV-QQANNTQLLEIA--GKQEQVYYEDSFDSLAFLNKNIMFEIC---NP--QESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVP-QELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSH 1400 HSP 6 Score: 76.2554 bits (186), Expect = 6.222e-14 Identity = 66/228 (28.95%), Postives = 110/228 (48.25%), Query Frame = 1
Query: 181 DDQNDQITSLTA-----ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT--MRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLA-SPGLSLQVESFTGLLKTQSQVVSHIC-PIPDPGLEKPCTLEQQDLVVILDSS 837
D+ +D IT + AC+ D++F++DGS SI+ +F AK F K+++ S RI ++ + + F Y M + + L ++T GK L + P RK V + +++ TDG +N + A L+ + V+I +IGIG N EL +A SP +VE+F L + Q+V+ +C P P + K C+L D+ + +D S
Sbjct: 1380 DNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSN--DSVNEDAAALRSSGVKIFSIGIGLR-NSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSL---DITISVDYS 1601 HSP 7 Score: 70.8626 bits (172), Expect = 3.639e-12 Identity = 75/357 (21.01%), Postives = 162/357 (45.38%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKAL---NLTL--KEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIG--IGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLISQQR------PNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE 1263
D++ +++ + + FE AK F D+++ + + RI + + D+ F +Y+ ++L + N + + T G+AL N T + + V +++++ T+G +++ P A +LK + + +G I D + E + Q++ F Q++ ++C D ++ + D+V ++DSS S+ +++ K+F++ ++ +G K+R+ + +S + +F Y ++++ +K + GG G AL V S ++ N F ++I + + R +A LK G IS+GIG + KEL+ I+++
Sbjct: 237 DIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQV-YSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRR---HAEELKSVGVTTISVGIGNS-DRKELEKIATD 585
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: ENSXETT00000014315.1 (collagen type VI alpha 5 chain [Source:NCBI gene;Acc:100486063]) HSP 1 Score: 118.627 bits (296), Expect = 1.680e-27 Identity = 101/385 (26.23%), Postives = 182/385 (47.27%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMM--TIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM--RKEVEKLVVLFTDGTNNMYPAPY--VYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKL--ISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK-ILTVETYGDLKKSLKTVMKTVIC--GNQP 1350
+ D++F+LD S SI++ +F L ++F + ++ +++ N + + +G + F TY+ +L V + + T KAL T + R + +++L TDG P + + LK+ + + +G+G + EL ++A P V+++ GL + +C P C+ EQ DLV ++D S SI+ ++ K F+ + + +F + + R+ + +S + +F +YY S DM ++ S+ + T GK LR V + R NVP ++++TDG++N E A L+ G I S+GIG N EL MI+ K + VET+ L S+K + +C +QP
Sbjct: 1211 VADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQ--FGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVP-QELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPA----CSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVN--EDAAALRSSGVKIFSIGIGLR-NSFELVMIAGSPKNVYEVETFQAL-DSIKRQIVAQVCEPSDQP 1584 HSP 2 Score: 107.842 bits (268), Expect = 5.278e-24 Identity = 91/396 (22.98%), Postives = 193/396 (48.74%), Query Frame = 1
Query: 187 QNDQITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM-----RKEVEKLVVLFTDGTN-NMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS--PGLSL-QVESFTGLLKTQSQVVSHIC----------PIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI--SQQRPN-VPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLK 1302
Q ++ + A C+ + D++F++D S SI +F+L + F +++ + S + R+ + + D+ + + T+ + ++L +T G AL+ K+++ + V+++ V+ T+G + + + P A KL+ + V++ +G +IN EL +AS P + VESF L + ++ +C P +++ C E+ D+ ++D S SI ED+E K F+++ +S F++G + R ++ +SD R +F +++ + + + + + GG T G+AL + + ++ RP+ VP ++++ITDG + R E A ++ G I ++G+ + + + S +K+ V + LK
Sbjct: 615 QFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGF-QNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQ--DRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLK 1004 HSP 3 Score: 94.3597 bits (233), Expect = 1.223e-19 Identity = 88/384 (22.92%), Postives = 177/384 (46.09%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE----VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGI-----GSDININELTR--LASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
D+ F++DGS SI +DFE KKF +++++ + R +++ D F + K + ++ L T G+AL ++K+++ K+ V + +V+ TDG + D++ EA +I GI G + E R S V +F L ++ + +C + C + D+V ++DSS SI+ +DYE K F+ + E+GP++ ++ ++ FS E + +F Y + D++ + ++ + + T +G+AL+ + + R N +++ITDG + P A ++ G I ++G+ + N + L++ ++++ +++ L K +M IC Q
Sbjct: 840 DIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALT-SMKQLFVNAAKDRPHKVPQSLVVITDGESQ---------DRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELC------TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFE-ICNPQ 1203 HSP 4 Score: 90.5077 bits (223), Expect = 2.399e-18 Identity = 90/388 (23.20%), Postives = 177/388 (45.62%), Query Frame = 1
Query: 118 MNLFLAVLPILIGFFTFNVKTDDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP-----TMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVES---FTGLLKTQSQVVSHICPIPDPG------LEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ-----RPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLG 1215
M + L ++G ++ NV Q T T A I D++F++DGS SI ++F K F ++ + +I I++ D+A F +Y+ + +L + N + T G +L L + + + V ++ V+ TDG + P + +K A + + IGI D ++EL +AS V S F L ++ +C + + + C T Q D+V++++S+ + + +E+ KNFL +SN ++G K R+ +++++DE +F Y +++ ++++ G T G+AL V++ +Q +V ++I+T+G ++ P A LK +G ++ +G
Sbjct: 1 MGFWKVFLACVLGSYSVNVSAS----QKTVCTEAS---IADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAI---AVKNAGIILYAIGI-KDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVG 374 HSP 5 Score: 83.1889 bits (204), Expect = 4.855e-16 Identity = 91/394 (23.10%), Postives = 177/394 (44.92%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM--RKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQV---ESFTGLLKTQSQVVSHICPIPDPGLEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQ--QRPNVPFNILIITDGVNNMFPRP-LEYANR-LKMKGANIISLGIGEEINLKELKMISS-EDKILTVETYGDLKKSLKTVMKTVICGNQPQVEY 1362
AC + D++F++D S SI+ DD+E K+F + M+ + +I I+F + + F Y + ++ + + L T +G+AL TL + R ++ +++ TDG + P A +++ V I IG+ N +L +A G QV +SF L ++ IC +P ++ C E D++ +LD+S SI+ ++ + F+ +++ +G + + + + F Y D++ V SL + G T KAL + + RP + ++++TDG RP L ++ LK G + ++G+G+ + +EL+ I+ D+ V+ Y L + + V ++P +
Sbjct: 1019 ACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGV-QQANNTQLLEIA--GKQEQVYYEDSFDSLAFLNKNIMFEIC---NP--QESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVP-QELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSH 1400 HSP 6 Score: 76.2554 bits (186), Expect = 6.328e-14 Identity = 66/228 (28.95%), Postives = 110/228 (48.25%), Query Frame = 1
Query: 181 DDQNDQITSLTA-----ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT--MRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLA-SPGLSLQVESFTGLLKTQSQVVSHIC-PIPDPGLEKPCTLEQQDLVVILDSS 837
D+ +D IT + AC+ D++F++DGS SI+ +F AK F K+++ S RI ++ + + F Y M + + L ++T GK L + P RK V + +++ TDG +N + A L+ + V+I +IGIG N EL +A SP +VE+F L + Q+V+ +C P P + K C+L D+ + +D S
Sbjct: 1380 DNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSN--DSVNEDAAALRSSGVKIFSIGIGLR-NSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSL---DITISVDYS 1601 HSP 7 Score: 70.8626 bits (172), Expect = 3.084e-12 Identity = 75/357 (21.01%), Postives = 162/357 (45.38%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKAL---NLTL--KEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIG--IGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLISQQR------PNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE 1263
D++ +++ + + FE AK F D+++ + + RI + + D+ F +Y+ ++L + N + + T G+AL N T + + V +++++ T+G +++ P A +LK + + +G I D + E + Q++ F Q++ ++C D ++ + D+V ++DSS S+ +++ K+F++ ++ +G K+R+ + +S + +F Y ++++ +K + GG G AL V S ++ N F ++I + + R +A LK G IS+GIG + KEL+ I+++
Sbjct: 237 DIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQV-YSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRR---HAEELKSVGVTTISVGIGNS-DRKELEKIATD 585
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: ENSXETT00000014273.1 (collagen type VI alpha 5 chain [Source:NCBI gene;Acc:100486063]) HSP 1 Score: 118.627 bits (296), Expect = 1.881e-27 Identity = 101/385 (26.23%), Postives = 182/385 (47.27%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMM--TIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM--RKEVEKLVVLFTDGTNNMYPAPY--VYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKL--ISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK-ILTVETYGDLKKSLKTVMKTVIC--GNQP 1350
+ D++F+LD S SI++ +F L ++F + ++ +++ N + + +G + F TY+ +L V + + T KAL T + R + +++L TDG P + + LK+ + + +G+G + EL ++A P V+++ GL + +C P C+ EQ DLV ++D S SI+ ++ K F+ + + +F + + R+ + +S + +F +YY S DM ++ S+ + T GK LR V + R NVP ++++TDG++N E A L+ G I S+GIG N EL MI+ K + VET+ L S+K + +C +QP
Sbjct: 1211 VADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQ--FGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVP-QELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPA----CSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVN--EDAAALRSSGVKIFSIGIGLR-NSFELVMIAGSPKNVYEVETFQAL-DSIKRQIVAQVCEPSDQP 1584 HSP 2 Score: 107.842 bits (268), Expect = 6.120e-24 Identity = 90/392 (22.96%), Postives = 192/392 (48.98%), Query Frame = 1
Query: 199 ITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM-----RKEVEKLVVLFTDGTN-NMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS--PGLSL-QVESFTGLLKTQSQVVSHIC----------PIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI--SQQRPN-VPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLK 1302
++ + A C+ + D++F++D S SI +F+L + F +++ + S + R+ + + D+ + + T+ + ++L +T G AL+ K+++ + V+++ V+ T+G + + + P A KL+ + V++ +G +IN EL +AS P + VESF L + ++ +C P +++ C E+ D+ ++D S SI ED+E K F+++ +S F++G + R ++ +SD R +F +++ + + + + + GG T G+AL + + ++ RP+ VP ++++ITDG + R E A ++ G I ++G+ + + + S +K+ V + LK
Sbjct: 619 VSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGF-QNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQ--DRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLK 1004 HSP 3 Score: 94.3597 bits (233), Expect = 1.271e-19 Identity = 85/376 (22.61%), Postives = 174/376 (46.28%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE----VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGI-----GSDININELTR--LASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVM 1323
D+ F++DGS SI +DFE KKF +++++ + R +++ D F + K + ++ L T G+AL ++K+++ K+ V + +V+ TDG + D++ EA +I GI G + E R S V +F L ++ + +C + C + D+V ++DSS SI+ +DYE K F+ + E+GP++ ++ ++ FS E + +F Y + D++ + ++ + + T +G+AL+ + + R N +++ITDG + P A ++ G I ++G+ + N + L++ ++++ +++ L K +M
Sbjct: 840 DIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALT-SMKQLFVNAAKDRPHKVPQSLVVITDGESQ---------DRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELC------TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIM 1196 HSP 4 Score: 90.5077 bits (223), Expect = 2.146e-18 Identity = 90/388 (23.20%), Postives = 177/388 (45.62%), Query Frame = 1
Query: 118 MNLFLAVLPILIGFFTFNVKTDDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP-----TMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVES---FTGLLKTQSQVVSHICPIPDPG------LEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ-----RPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLG 1215
M + L ++G ++ NV Q T T A I D++F++DGS SI ++F K F ++ + +I I++ D+A F +Y+ + +L + N + T G +L L + + + V ++ V+ TDG + P + +K A + + IGI D ++EL +AS V S F L ++ +C + + + C T Q D+V++++S+ + + +E+ KNFL +SN ++G K R+ +++++DE +F Y +++ ++++ G T G+AL V++ +Q +V ++I+T+G ++ P A LK +G ++ +G
Sbjct: 1 MGFWKVFLACVLGSYSVNVSAS----QKTVCTEAS---IADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAI---AVKNAGIILYAIGI-KDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVG 374 HSP 5 Score: 83.1889 bits (204), Expect = 4.957e-16 Identity = 91/393 (23.16%), Postives = 175/393 (44.53%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM--RKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQV---ESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQ--QRPNVPFNILIITDGVNNMFPRP-LEYANR-LKMKGANIISLGIGEEINLKELKMISS-EDKILTVETYGDLKKSLKTVMKTVICGNQPQVEY 1362
AC + D++F++D S SI+ DD+E K+F + M+ + +I I+F + + F Y + ++ + + L T +G+AL TL + R ++ +++ TDG + P A +++ V I IG+ N +L +A G QV +SF L ++ IC P +K E D++ +LD+S SI+ ++ + F+ +++ +G + + + + F Y D++ V SL + G T KAL + + RP + ++++TDG RP L ++ LK G + ++G+G+ + +EL+ I+ D+ V+ Y L + + V ++P +
Sbjct: 1019 ACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGV-QQANNTQLLEIA--GKQEQVYYEDSFDSLAFLNKNIMFEICN-PQESCKK---TEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVP-QELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESKPACSH 1400 HSP 6 Score: 76.2554 bits (186), Expect = 6.130e-14 Identity = 66/228 (28.95%), Postives = 110/228 (48.25%), Query Frame = 1
Query: 181 DDQNDQITSLTA-----ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT--MRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLA-SPGLSLQVESFTGLLKTQSQVVSHIC-PIPDPGLEKPCTLEQQDLVVILDSS 837
D+ +D IT + AC+ D++F++DGS SI+ +F AK F K+++ S RI ++ + + F Y M + + L ++T GK L + P RK V + +++ TDG +N + A L+ + V+I +IGIG N EL +A SP +VE+F L + Q+V+ +C P P + K C+L D+ + +D S
Sbjct: 1380 DNIHDNITQVVCDESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSN--DSVNEDAAALRSSGVKIFSIGIGLR-NSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECSL---DITISVDYS 1601 HSP 7 Score: 70.8626 bits (172), Expect = 3.258e-12 Identity = 75/357 (21.01%), Postives = 162/357 (45.38%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKAL---NLTL--KEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIG--IGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLISQQR------PNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE 1263
D++ +++ + + FE AK F D+++ + + RI + + D+ F +Y+ ++L + N + + T G+AL N T + + V +++++ T+G +++ P A +LK + + +G I D + E + Q++ F Q++ ++C D ++ + D+V ++DSS S+ +++ K+F++ ++ +G K+R+ + +S + +F Y ++++ +K + GG G AL V S ++ N F ++I + + R +A LK G IS+GIG + KEL+ I+++
Sbjct: 237 DIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQV-YSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRR---HAEELKSVGVTTISVGIGNS-DRKELEKIATD 585
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: VWA2 (von Willebrand factor A domain containing 2 [Source:NCBI gene;Acc:101733495]) HSP 1 Score: 102.064 bits (253), Expect = 2.361e-22 Identity = 86/382 (22.51%), Postives = 173/382 (45.29%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK------EVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTG---LLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ---RPNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
D+LF++D S + S + F L K F K + + + + ++ ++ ++V + Y ++L V N R + GKAL + V+ +R ++ +LVVL + + + P Y + ++ IG+ D N E+ + G LQ+ +++ L Q+ IC I G C + DL +LD+S ++ +E + + KNF+ F++ + ++ ++++S + FG + ++ + GG+ IG AL V +++++ Q RP V +++I+DG + P A +L+ G + +GIG + L++ +E ++TV +Y L +V++ V
Sbjct: 307 DLLFLVDSSDATSLEGFMLHKSFLKQFLQASLSDESPVNVGVAQYSNEVQMVVKIGEYQSTAELLKHVDNMRFMGGGLFTGKALRYVTQYGFKSSPVFSDIRDDLPRLVVLLSGSKSQDSVAGPATYTR-----DQEVFLIGVAGDSNKGEMAEII--GNPLQLITYSNPQQLFNQLPQLQKRICSIDVQG----CQAQPLDLAFVLDASAAVGQEKFNRLKNFVTMVSLQFDINRDVTQIGLVTYSSRPQTVFGLDTHDSGSGLLQGIGRASYIGGSASIGNALLHVYNEVMTVQKGARPGVNKAVVVISDGRGAEDAAVP-----AQKLRDNGIMVYVIGIGSILRSALLRLAGTEKFLITVPSYESLGHYEDSVVQRVC 672 HSP 2 Score: 65.855 bits (159), Expect = 9.683e-11 Identity = 51/188 (27.13%), Postives = 91/188 (48.40%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQR-PNVPFNILIITDG---VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS---EDKILTVETYGDLKKSLKTVMK-TVICGNQP 1350
D++++LD SNSI +E+ K+F K ++G + RV + +S + +F + D+ K+KS++ GG T G AL+ + K R P VP ++I++DG N P A ++K + + ++G+ + +EL +SS E +L E D L T + + +C + P
Sbjct: 15 DILILLDGSNSIGRGSFERSKHFASKLCDALDIGSDLVRVGAMQYSGTPQVEFHLDSSFSKADIKEKIKSIVFKGGPTETGLALKYIVWKGFPGARPPPVPKILIIVSDGKSQGNIKLP-----AAQIKGEDIEVFTVGV-KFPRWEELHALSSEPMESHVLFAEHVDDAVNGLATSLANSSVCSSTP 196
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: VWA2 (von Willebrand factor A domain containing 2 [Source:NCBI gene;Acc:101733495]) HSP 1 Score: 101.679 bits (252), Expect = 3.146e-22 Identity = 86/382 (22.51%), Postives = 173/382 (45.29%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK------EVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTG---LLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ---RPNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
D+LF++D S + S + F L K F K + + + + ++ ++ ++V + Y ++L V N R + GKAL + V+ +R ++ +LVVL + + + P Y + ++ IG+ D N E+ + G LQ+ +++ L Q+ IC I G C + DL +LD+S ++ +E + + KNF+ F++ + ++ ++++S + FG + ++ + GG+ IG AL V +++++ Q RP V +++I+DG + P A +L+ G + +GIG + L++ +E ++TV +Y L +V++ V
Sbjct: 342 DLLFLVDSSDATSLEGFMLHKSFLKQFLQASLSDESPVNVGVAQYSNEVQMVVKIGEYQSTAELLKHVDNMRFMGGGLFTGKALRYVTQYGFKSSPVFSDIRDDLPRLVVLLSGSKSQDSVAGPATYTR-----DQEVFLIGVAGDSNKGEMAEII--GNPLQLITYSNPQQLFNQLPQLQKRICSIDVQG----CQAQPLDLAFVLDASAAVGQEKFNRLKNFVTMVSLQFDINRDVTQIGLVTYSSRPQTVFGLDTHDSGSGLLQGIGRASYIGGSASIGNALLHVYNEVMTVQKGARPGVNKAVVVISDGRGAEDAAVP-----AQKLRDNGIMVYVIGIGSILRSALLRLAGTEKFLITVPSYESLGHYEDSVVQRVC 707 HSP 2 Score: 65.4698 bits (158), Expect = 1.081e-10 Identity = 51/188 (27.13%), Postives = 91/188 (48.40%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQR-PNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS---EDKILTVETYGDLKKSLKTVMK-TVICGNQP 1350
D++++LD SNSI +E+ K+F K ++G + RV + +S + +F + D+ K+KS++ GG T G AL+ + K R P VP ++I++DG N P A ++K + + ++G+ + +EL +SS E +L E D L T + + +C + P
Sbjct: 50 DILILLDGSNSIGRGSFERSKHFASKLCDALDIGSDLVRVGAMQYSGTPQVEFHLDSSFSKADIKEKIKSIVFKGGPTETGLALKYIVWKGFPGARPPPVPKILIIVSDGKSQGNIKLP-----AAQIKGEDIEVFTVGV-KFPRWEELHALSSEPMESHVLFAEHVDDAVNGLATSLANSSVCSSTP 231
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a6 (collagen, type VI, alpha 6 [Source:MGI Symbol;Acc:MGI:2444259]) HSP 1 Score: 127.872 bits (320), Expect = 1.037e-30 Identity = 106/395 (26.84%), Postives = 201/395 (50.89%), Query Frame = 1
Query: 199 ITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNT-ENRIAF--IKFGDDAEIVF---EFQTYTKLKQMLL-DVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQ---VESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
I S C I + DV+F++D S SI ++ + K F M+ ++K ++ +N++ F +K+ DD E++F E T ++ +L D N Y++ + + ++ + + K V +++++ TDG ++ A L++ + +L +GI N LA G S + VE+F GL S V + +C + C +E+ DLV ++D SNSI +D+++ K FLV + +F++ + R+ + FSD R +F + ++ +++ + + G T IG ALR+V + S+ P +L++TDG + + A L+ KG +I S+GIG+ + + +++ + +K LTV + +LKK K +++ IC
Sbjct: 794 ICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDF---MIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAG---ANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSS----KVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQ--DEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRN-IC 1175 HSP 2 Score: 102.064 bits (253), Expect = 2.414e-22 Identity = 95/381 (24.93%), Postives = 180/381 (47.24%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP--TMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGI-GSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTV 1332
AC + D++F++D S SI ++F K F K++++ + +I ++F + + F+ T+ + + ++ +T G AL + P R V K ++L TDG ++ P + L++ V I ++G+ GS N+ +L ++ P + VE+F L + +V IC P E+ +E D+V ++DSS SI ++Y K+F++ + ++G + R L ++D+ F + ++++ +++ GG T +AL D S+ VP +++ITDG ++ + A L+ KG ++++GI + + L M S DK VET+G LK V +V
Sbjct: 614 ACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAI---ALRKEGVIIYSVGVFGS--NVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICS-PR---EECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASV 985 HSP 3 Score: 91.6633 bits (226), Expect = 5.970e-19 Identity = 86/400 (21.50%), Postives = 180/400 (45.00%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTD--GTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSH-----------------ICPIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALR---EVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
+ DV+F+LD + + S++D + K F + ++ + R+ + + ++ ++ T ++L + + G AL T KE++ R + V ++ VL T +N+ A A L+ V I T+GI N +EL ++AS E FT L S++ +H + L+ C E+ D+ +++D S S D+ + K FL + + F + P K RV + ++D +F + Y D+ ++++ + GG T G AL ++ + ++ VP +++++T+G++ L A++L+ + + ++G+ +E N +L+ I+ E+K + D ++++ + IC +
Sbjct: 226 VADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKA----AVNLRREGVTIFTMGI-EGANPDELEKIASH----PAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSR--DSVLGPAHKLREENIRVHAIGV-KEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEE 613 HSP 4 Score: 56.225 bits (134), Expect = 8.207e-8 Identity = 48/170 (28.24%), Postives = 77/170 (45.29%), Query Frame = 1
Query: 754 HICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS---QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKEL 1245
H+C D G P E D+V ++DSS+ + + + K F+ K +S+ + K RVA+ +SD + +F + + M+N +K F GG+ IG AL+E S R F +++ E A L+ G IIS+G+ E NLK +
Sbjct: 13 HVCVNQDSG---P---EYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAM 176
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a4 (collagen, type VI, alpha 4 [Source:MGI Symbol;Acc:MGI:1915803]) HSP 1 Score: 127.102 bits (318), Expect = 1.956e-30 Identity = 96/374 (25.67%), Postives = 178/374 (47.59%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDGV--NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
D+ F++DGS SI +DF K F K+++ + R +++ D F Y + + + N + + T GKAL+ + T R +V + +++ TDG + A A L++ V I IG+ D N EL +AS + E F L +V+ IC + C ++ D++ ++D S SI+ +D+E+ K+F+ + ++ +G ++ ++ +L FS + +F Y DM + S+ + T GKAL S + RP V +++ITDGV +N+ P A L+ + I ++G+G + L++ + +DK+ E + L +SL+ + + +C +Q
Sbjct: 849 DIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQSTDPVAEA--AQGLRDIGVNIYAIGV-RDANTTELEEIASKKMFFIYE-FDSLKSIHQEVIRDICS------SENCKSQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFSSNPQEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKALNFTLPFFDSSRGGRPRVHQYLIVITDGVSQDNVAPP----AKALRDRNIIIFAIGVGNVQRAQLLEITNDQDKVFQEENFESL-QSLEKEILSEVCSSQ 1207 HSP 2 Score: 98.5969 bits (244), Expect = 3.941e-21 Identity = 88/356 (24.72%), Postives = 176/356 (49.44%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNL-YS--STGIGKALNL----TLKEVYPTMRKE-VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLI-----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMI 1254
D++F++D S SI +F+ K F +++ + + + ++ +++ D+ F + + LK +LD RNL YS T G AL +L E+ + K+ V ++VVL TDG ++ AD+LK V + +GI ++ EL ++A+ E+F+ L ++ +C + ++K T D+V ++D+S S+ ++ +NF+ + + E+G +K ++ + +SD+ +F F + ++M+ + L+ F GG+ G+ LR + S+ VP +++IT G + E A L+ +G +I+S+G+ ++ + EL+ I
Sbjct: 235 DIVFLVDSSTSIGLQNFQKVKHFLHSVVSGLDVRSDQVQVGLVQYSDNIYPAFPLKQ-SSLKSAVLD--RIRNLPYSMGGTSTGSALEFIRANSLTEMSGSRAKDGVPQIVVLVTDGESS--DEVQDVADQLKRDGVFVFVVGINIQ-DVQELQKIANEPFEEFLFTTENFSILQALSGTLLQALCSTVERQMKKS-TKTYADVVFLIDTSQGTSQASFQWMQNFISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRFLHRTFFQEAAGSRLLQGVPQYVVVITSGKSE--DEVGEVAQILRKRGVDIVSVGL-QDFDRAELEGI 580 HSP 3 Score: 93.5893 bits (231), Expect = 1.582e-19 Identity = 92/400 (23.00%), Postives = 183/400 (45.75%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQML--LDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTDGT---NNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGL--SLQVESFTGLLKTQSQVVSHICP------------IPDP---GLEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPL-EYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
D++F+++ S + +F+ F + + R++ + + + + F Y Q+L LD R G AL+ KEV+ + + V+++ V+ + N PA Y L+ A V I G +L ++ + P ++++ESF L +++ +CP IP+ ++ C ++E+ D+ ++D S SI D+ + K+F+ + + F +GP++ R ++ +SD+I F T Y + + ++ + GG T GKAL ++ + R +V +++ITDG + P+ E A L+ G NI ++G+ + N EL+ I+S+ K+ + + LK + V++ IC ++
Sbjct: 634 DLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLEFSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRGVQQIAVVIIESPSLDNVSTPASY-----LRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNKLKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQST---DPVAEAAQGLRDIGVNIYAIGV-RDANTTELEEIASK-KMFFIYEFDSLKSIHQEVIRD-ICSSE 1022
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a6 (collagen, type VI, alpha 6 [Source:MGI Symbol;Acc:MGI:2444259]) HSP 1 Score: 126.331 bits (316), Expect = 3.565e-30 Identity = 106/395 (26.84%), Postives = 201/395 (50.89%), Query Frame = 1
Query: 199 ITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNT-ENRIAF--IKFGDDAEIVF---EFQTYTKLKQMLL-DVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQ---VESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
I S C I + DV+F++D S SI ++ + K F M+ ++K ++ +N++ F +K+ DD E++F E T ++ +L D N Y++ + + ++ + + K V +++++ TDG ++ A L++ + +L +GI N LA G S + VE+F GL S V + +C + C +E+ DLV ++D SNSI +D+++ K FLV + +F++ + R+ + FSD R +F + ++ +++ + + G T IG ALR+V + S+ P +L++TDG + + A L+ KG +I S+GIG+ + + +++ + +K LTV + +LKK K +++ IC
Sbjct: 794 ICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDF---MIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIA---GANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSS----KVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQ--DEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRN-IC 1175 HSP 2 Score: 100.908 bits (250), Expect = 8.085e-22 Identity = 95/382 (24.87%), Postives = 180/382 (47.12%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP--TMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGI-GSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
AC + D++F++D S SI ++F K F K++++ + +I ++F + + F+ T+ + + ++ +T G AL + P R V K ++L TDG ++ P + L++ V I ++G+ GS N+ +L ++ P + VE+F L + +V IC P E+ +E D+V ++DSS SI ++Y K+F++ + ++G + R L ++D+ F + ++++ +++ GG T +AL D S+ VP +++ITDG ++ + A L+ KG ++++GI + + L M S DK VET+G LK V +V
Sbjct: 614 ACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAI---ALRKEGVIIYSVGVFGS--NVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGIC---SP-REECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVC 986 HSP 3 Score: 90.5077 bits (223), Expect = 1.345e-18 Identity = 86/400 (21.50%), Postives = 180/400 (45.00%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTD--GTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSH-----------------ICPIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALR---EVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
+ DV+F+LD + + S++D + K F + ++ + R+ + + ++ ++ T ++L + + G AL T KE++ R + V ++ VL T +N+ A A L+ V I T+GI N +EL ++AS E FT L S++ +H + L+ C E+ D+ +++D S S D+ + K FL + + F + P K RV + ++D +F + Y D+ ++++ + GG T G AL ++ + ++ VP +++++T+G++ L A++L+ + + ++G+ +E N +L+ I+ E+K + D ++++ + IC +
Sbjct: 226 VADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKA----AVNLRREGVTIFTMGI-EGANPDELEKIAS----HPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSR--DSVLGPAHKLREENIRVHAIGV-KEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEE 613 HSP 4 Score: 54.299 bits (129), Expect = 3.579e-7 Identity = 33/119 (27.73%), Postives = 62/119 (52.10%), Query Frame = 1
Query: 775 PGLE-KP-CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFEL----GPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLI--KFGGATGIGKALREVDSKLISQQRPNV 1107
PG KP C + +LV +LD S ++E+D+E+ K +V + + ++ P RVA+L+++ R F+D Y+ D ++ +K+L + + IGKA+R + + + P
Sbjct: 1742 PGRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMVSLVRDVKVREANCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLTAIKALPYERSSDSREIGKAMRFISRNVFKRTLPGA 1860 HSP 5 Score: 53.9138 bits (128), Expect = 5.178e-7 Identity = 47/170 (27.65%), Postives = 76/170 (44.71%), Query Frame = 1
Query: 754 HICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS---QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKEL 1245
H+C D G E D+V ++DSS+ + + + K F+ K +S+ + K RVA+ +SD + +F + + M+N +K F GG+ IG AL+E S R F +++ E A L+ G IIS+G+ E NLK +
Sbjct: 13 HVCVNQDSGPE------YADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAM 176
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a6 (collagen, type VI, alpha 6 [Source:MGI Symbol;Acc:MGI:2444259]) HSP 1 Score: 126.331 bits (316), Expect = 3.907e-30 Identity = 106/395 (26.84%), Postives = 201/395 (50.89%), Query Frame = 1
Query: 199 ITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNT-ENRIAF--IKFGDDAEIVF---EFQTYTKLKQMLL-DVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQ---VESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
I S C I + DV+F++D S SI ++ + K F M+ ++K ++ +N++ F +K+ DD E++F E T ++ +L D N Y++ + + ++ + + K V +++++ TDG ++ A L++ + +L +GI N LA G S + VE+F GL S V + +C + C +E+ DLV ++D SNSI +D+++ K FLV + +F++ + R+ + FSD R +F + ++ +++ + + G T IG ALR+V + S+ P +L++TDG + + A L+ KG +I S+GIG+ + + +++ + +K LTV + +LKK K +++ IC
Sbjct: 794 ICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDF---MIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIA---GANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSS----KVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQ--DEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRN-IC 1175 HSP 2 Score: 100.523 bits (249), Expect = 8.684e-22 Identity = 95/382 (24.87%), Postives = 180/382 (47.12%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP--TMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGI-GSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
AC + D++F++D S SI ++F K F K++++ + +I ++F + + F+ T+ + + ++ +T G AL + P R V K ++L TDG ++ P + L++ V I ++G+ GS N+ +L ++ P + VE+F L + +V IC P E+ +E D+V ++DSS SI ++Y K+F++ + ++G + R L ++D+ F + ++++ +++ GG T +AL D S+ VP +++ITDG ++ + A L+ KG ++++GI + + L M S DK VET+G LK V +V
Sbjct: 614 ACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAI---ALRKEGVIIYSVGVFGS--NVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGIC---SP-REECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVC 986 HSP 3 Score: 90.5077 bits (223), Expect = 1.345e-18 Identity = 86/400 (21.50%), Postives = 180/400 (45.00%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTD--GTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSH-----------------ICPIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALR---EVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
+ DV+F+LD + + S++D + K F + ++ + R+ + + ++ ++ T ++L + + G AL T KE++ R + V ++ VL T +N+ A A L+ V I T+GI N +EL ++AS E FT L S++ +H + L+ C E+ D+ +++D S S D+ + K FL + + F + P K RV + ++D +F + Y D+ ++++ + GG T G AL ++ + ++ VP +++++T+G++ L A++L+ + + ++G+ +E N +L+ I+ E+K + D ++++ + IC +
Sbjct: 226 VADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKA----AVNLRREGVTIFTMGI-EGANPDELEKIAS----HPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSR--DSVLGPAHKLREENIRVHAIGV-KEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEE 613 HSP 4 Score: 53.9138 bits (128), Expect = 4.876e-7 Identity = 31/114 (27.19%), Postives = 60/114 (52.63%), Query Frame = 1
Query: 787 KP-CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFEL----GPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLI--KFGGATGIGKALREVDSKLISQQRPNV 1107
KP C + +LV +LD S ++E+D+E+ K +V + + ++ P RVA+L+++ R F+D Y+ D ++ +K+L + + IGKA+R + + + P
Sbjct: 1747 KPECPVHPTELVFVLDQSRDVTEQDFERMKGMMVSLVRDVKVREANCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLTAIKALPYERSSDSREIGKAMRFISRNVFKRTLPGA 1860 HSP 5 Score: 53.9138 bits (128), Expect = 5.046e-7 Identity = 47/170 (27.65%), Postives = 76/170 (44.71%), Query Frame = 1
Query: 754 HICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS---QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKEL 1245
H+C D G E D+V ++DSS+ + + + K F+ K +S+ + K RVA+ +SD + +F + + M+N +K F GG+ IG AL+E S R F +++ E A L+ G IIS+G+ E NLK +
Sbjct: 13 HVCVNQDSGPE------YADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAM 176
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Vwa2 (von Willebrand factor A domain containing 2 [Source:MGI Symbol;Acc:MGI:2684334]) HSP 1 Score: 104.76 bits (260), Expect = 2.803e-23 Identity = 81/368 (22.01%), Postives = 161/368 (43.75%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRK----EVEKLVVLFTDGTNN---MYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQ-SQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ---RPNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDL 1299
DVLF+LD S + F AK F K + + ++ R+ +G + + Y + ++ + + T G AL + + + + ++VVL T+ + PA + A +L L +G+GS+I EL ++ + V + L +Q ++ +C P PG C + DLV +LD+S S+ E++ Q ++F+ K F++ P+ +V ++ + ++ FG + ++ + GG G AL ++ K+++ Q RP VP ++++T G + P A +L+ G +++ + +G + ++ D ++ V Y DL
Sbjct: 342 DVLFLLDSSAGTTLGGFRRAKAFVKRFVQAVLREDSRARVGIASYGRNLMVAVPVGEYQHVPDLIRSLDSIPFSGGPTLTGSALLQVAEHGFGSASRTGQDRPRRVVVLLTESRSQDEVSGPAAHARAREL-------LLLGVGSEILQAELVKITGSPKHVMVHTDPQDLFSQIPELQRRLCSQPRPG----CQAQSLDLVFLLDASASVGRENFAQMQSFIRKCTLRFDVNPDVTQVGLVVYGSRVQTAFGLDTHPTRAAVLRAMSQAPYLGGVGSAGTALLHIEDKVMTVQRGARPGVPKAVVMLTGGSGAEDAAVP-----AQKLRGNGISVLVMSVGAVLREAVRRLAGPRDSLIHVAAYTDL 693 HSP 2 Score: 60.4622 bits (145), Expect = 3.073e-9 Identity = 39/153 (25.49%), Postives = 74/153 (48.37%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK 1269
D++ +LD S+SI + +E+ K F + ++ P + RV L F +F + ++ +K ++ GG T G AL+ + + +VP ++I+TDG + P L A +L+ +G + ++G+ EL ++SE K
Sbjct: 51 DILFLLDGSHSIGKGSFERSKRFAIAACDALDISPGRVRVGALQFGSTPHLEFPLDSFSTRQEVKESIKGIVFKGGRTETGLALKRLSRGFPGGRNGSVPQILIIVTDG-KSQGPVALP-AKQLRERGIVVFAVGV-RFPRWDELLTLASEPK 200 HSP 3 Score: 54.299 bits (129), Expect = 3.033e-7 Identity = 40/151 (26.49%), Postives = 71/151 (47.02%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRK-EVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS 687
D+LF+LDGS SI K FE +K+F + S R+ ++FG + F +++ +++ + T G AL L +P R V +++++ TDG + A + A +L+E + + +G+ +EL LAS
Sbjct: 51 DILFLLDGSHSIGKGSFERSKRFAIAACDALDISPGRVRVGALQFGSTPHLEFPLDSFSTRQEVKESIKGIVFKGGRTETGLALK-RLSRGFPGGRNGSVPQILIIVTDGKSQGPVA--LPAKQLRERGIVVFAVGV-RFPRWDELLTLAS 197
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|A2AX52|CO6A4_MOUSE (Collagen alpha-4(VI) chain OS=Mus musculus OX=10090 GN=Col6a4 PE=1 SV=2) HSP 1 Score: 127.102 bits (318), Expect = 1.368e-29 Identity = 96/374 (25.67%), Postives = 178/374 (47.59%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDGV--NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
D+ F++DGS SI +DF K F K+++ + R +++ D F Y + + + N + + T GKAL+ + T R +V + +++ TDG + A A L++ V I IG+ D N EL +AS + E F L +V+ IC + C ++ D++ ++D S SI+ +D+E+ K+F+ + ++ +G ++ ++ +L FS + +F Y DM + S+ + T GKAL S + RP V +++ITDGV +N+ P A L+ + I ++G+G + L++ + +DK+ E + L +SL+ + + +C +Q
Sbjct: 849 DIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQSTDPVAEA--AQGLRDIGVNIYAIGV-RDANTTELEEIASKKMFFIYE-FDSLKSIHQEVIRDICS------SENCKSQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFSSNPQEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKALNFTLPFFDSSRGGRPRVHQYLIVITDGVSQDNVAPP----AKALRDRNIIIFAIGVGNVQRAQLLEITNDQDKVFQEENFESL-QSLEKEILSEVCSSQ 1207 HSP 2 Score: 98.5969 bits (244), Expect = 2.757e-20 Identity = 88/356 (24.72%), Postives = 176/356 (49.44%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNL-YS--STGIGKALNL----TLKEVYPTMRKE-VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLI-----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMI 1254
D++F++D S SI +F+ K F +++ + + + ++ +++ D+ F + + LK +LD RNL YS T G AL +L E+ + K+ V ++VVL TDG ++ AD+LK V + +GI ++ EL ++A+ E+F+ L ++ +C + ++K T D+V ++D+S S+ ++ +NF+ + + E+G +K ++ + +SD+ +F F + ++M+ + L+ F GG+ G+ LR + S+ VP +++IT G + E A L+ +G +I+S+G+ ++ + EL+ I
Sbjct: 235 DIVFLVDSSTSIGLQNFQKVKHFLHSVVSGLDVRSDQVQVGLVQYSDNIYPAFPLKQ-SSLKSAVLD--RIRNLPYSMGGTSTGSALEFIRANSLTEMSGSRAKDGVPQIVVLVTDGESS--DEVQDVADQLKRDGVFVFVVGINIQ-DVQELQKIANEPFEEFLFTTENFSILQALSGTLLQALCSTVERQMKKS-TKTYADVVFLIDTSQGTSQASFQWMQNFISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRFLHRTFFQEAAGSRLLQGVPQYVVVITSGKSE--DEVGEVAQILRKRGVDIVSVGL-QDFDRAELEGI 580 HSP 3 Score: 93.5893 bits (231), Expect = 1.106e-18 Identity = 92/400 (23.00%), Postives = 183/400 (45.75%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQML--LDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTDGT---NNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGL--SLQVESFTGLLKTQSQVVSHICP------------IPDP---GLEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPL-EYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
D++F+++ S + +F+ F + + R++ + + + + F Y Q+L LD R G AL+ KEV+ + + V+++ V+ + N PA Y L+ A V I G +L ++ + P ++++ESF L +++ +CP IP+ ++ C ++E+ D+ ++D S SI D+ + K+F+ + + F +GP++ R ++ +SD+I F T Y + + ++ + GG T GKAL ++ + R +V +++ITDG + P+ E A L+ G NI ++G+ + N EL+ I+S+ K+ + + LK + V++ IC ++
Sbjct: 634 DLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLEFSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRGVQQIAVVIIESPSLDNVSTPASY-----LRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNKLKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQST---DPVAEAAQGLRDIGVNIYAIGV-RDANTTELEEIASK-KMFFIYEFDSLKSIHQEVIRD-ICSSE 1022
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|Q8C6K9|CO6A6_MOUSE (Collagen alpha-6(VI) chain OS=Mus musculus OX=10090 GN=Col6a6 PE=1 SV=2) HSP 1 Score: 126.331 bits (316), Expect = 2.494e-29 Identity = 106/395 (26.84%), Postives = 201/395 (50.89%), Query Frame = 1
Query: 199 ITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNT-ENRIAF--IKFGDDAEIVF---EFQTYTKLKQMLL-DVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQ---VESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
I S C I + DV+F++D S SI ++ + K F M+ ++K ++ +N++ F +K+ DD E++F E T ++ +L D N Y++ + + ++ + + K V +++++ TDG ++ A L++ + +L +GI N LA G S + VE+F GL S V + +C + C +E+ DLV ++D SNSI +D+++ K FLV + +F++ + R+ + FSD R +F + ++ +++ + + G T IG ALR+V + S+ P +L++TDG + + A L+ KG +I S+GIG+ + + +++ + +K LTV + +LKK K +++ IC
Sbjct: 794 ICSPREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDF---MIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIA---GANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSS----KVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQ--DEVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRN-IC 1175 HSP 2 Score: 100.908 bits (250), Expect = 5.655e-21 Identity = 95/382 (24.87%), Postives = 180/382 (47.12%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP--TMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGI-GSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
AC + D++F++D S SI ++F K F K++++ + +I ++F + + F+ T+ + + ++ +T G AL + P R V K ++L TDG ++ P + L++ V I ++G+ GS N+ +L ++ P + VE+F L + +V IC P E+ +E D+V ++DSS SI ++Y K+F++ + ++G + R L ++D+ F + ++++ +++ GG T +AL D S+ VP +++ITDG ++ + A L+ KG ++++GI + + L M S DK VET+G LK V +V
Sbjct: 614 ACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAI---ALRKEGVIIYSVGVFGS--NVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGIC---SP-REECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVC 986 HSP 3 Score: 90.5077 bits (223), Expect = 9.411e-18 Identity = 86/400 (21.50%), Postives = 180/400 (45.00%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTD--GTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSH-----------------ICPIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALR---EVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
+ DV+F+LD + + S++D + K F + ++ + R+ + + ++ ++ T ++L + + G AL T KE++ R + V ++ VL T +N+ A A L+ V I T+GI N +EL ++AS E FT L S++ +H + L+ C E+ D+ +++D S S D+ + K FL + + F + P K RV + ++D +F + Y D+ ++++ + GG T G AL ++ + ++ VP +++++T+G++ L A++L+ + + ++G+ +E N +L+ I+ E+K + D ++++ + IC +
Sbjct: 226 VADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKA----AVNLRREGVTIFTMGI-EGANPDELEKIAS----HPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSR--DSVLGPAHKLREENIRVHAIGV-KEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAEE 613 HSP 4 Score: 54.299 bits (129), Expect = 2.504e-6 Identity = 33/119 (27.73%), Postives = 62/119 (52.10%), Query Frame = 1
Query: 775 PGLE-KP-CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFEL----GPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLI--KFGGATGIGKALREVDSKLISQQRPNV 1107
PG KP C + +LV +LD S ++E+D+E+ K +V + + ++ P RVA+L+++ R F+D Y+ D ++ +K+L + + IGKA+R + + + P
Sbjct: 1742 PGRHGKPECPVHPTELVFVLDQSRDVTEQDFERMKGMMVSLVRDVKVREANCPVGARVAILAYNSHTRHLIRFSDAYRKDQLLTAIKALPYERSSDSREIGKAMRFISRNVFKRTLPGA 1860 HSP 5 Score: 53.9138 bits (128), Expect = 3.622e-6 Identity = 47/170 (27.65%), Postives = 76/170 (44.71%), Query Frame = 1
Query: 754 HICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS---QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKEL 1245
H+C D G E D+V ++DSS+ + + + K F+ K +S+ + K RVA+ +SD + +F + + M+N +K F GG+ IG AL+E S R F +++ E A L+ G IIS+G+ E NLK +
Sbjct: 13 HVCVNQDSGPE------YADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALREDGVKIISVGVQKASEENLKAM 176
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|A6NMZ7|CO6A6_HUMAN (Collagen alpha-6(VI) chain OS=Homo sapiens OX=9606 GN=COL6A6 PE=1 SV=2) HSP 1 Score: 121.709 bits (304), Expect = 7.729e-28 Identity = 100/393 (25.45%), Postives = 196/393 (49.87%), Query Frame = 1
Query: 199 ITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNT-ENRIAF--IKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDS----KLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
I S C I + DV+F++D S SI D++ + K F M+ ++K ++ +N++ F +K+ DD E++F + +++ + N + + ST +AL + ++ R K V +++++ TDG ++ A L++ + +L +GI + L S VE+F GL S V + +C + C +++ DLV ++D S SI D+++ K FL + +F++ + R+ FSD +F + ++ +++++ + G T IG ALREV+ + S+ P +L++TDG + + A L+ +G +I S+GIG+ + + +++ + +K LTV + +LKK K +++ IC
Sbjct: 795 ICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDF---MIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFS-DHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCN----SSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQ--DEVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRN-IC 1176 HSP 2 Score: 114.39 bits (285), Expect = 2.144e-25 Identity = 91/362 (25.14%), Postives = 174/362 (48.07%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVY-ADKLKEANVQILTIGIGSDININELTRLASPGLSLQ-VESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE-DKILTVETYGDLKK 1305
D+ ++DGS S DF K F +++ + + + R+ +++ D ++ FE Y+ + + + N R + +T G ALN TL + ++ K+ TN M + A++L+E ++++ IGI + N +L +A + V F L ++QVV IC E+ C + D++ ++DSS SI E++ + K F+ +S ++GP++ ++ ++ FSD + +F + D+ N + + G T G AL V + RPN+ +++ITDG ++ P A L+ +G I S+G+ N+ +L+ IS + + VE + L++
Sbjct: 436 DIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRLREEHIRVYAIGI-KEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEIC------TEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEP---AVVLRQEGVIIYSVGVFGS-NVTQLEEISGRPEMVFYVENFDILQR 786 HSP 3 Score: 107.071 bits (266), Expect = 4.918e-23 Identity = 98/382 (25.65%), Postives = 184/382 (48.17%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT--MRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGI-GSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
AC + D++F++D S SI ++F K F K++++ + +I ++F D + F+ + + + ++ +T G AL+ + PT R + K ++L TDG ++ P V L++ V I ++G+ GS N+ +L ++ P + VE+F L + + +V IC P E+ +E D+V ++DSS SI ++Y K+F++ + ++G + R L ++D+ F D+ ++I+ +++ GG+T +AL D S+ VP +++ITDG ++ + A L+ KG ++++GI ++ L M S DK VET+G LK V +V
Sbjct: 615 ACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVV---LRQEGVIIYSVGVFGS--NVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGIC---SP-REECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVC 987 HSP 4 Score: 94.7449 bits (234), Expect = 5.468e-19 Identity = 94/390 (24.10%), Postives = 180/390 (46.15%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM---RKE--VEKLVVLFT--DGTNNMYPAPYVYADKLKEANVQILTIGI--GSDININELTRLASPGLSLQVESFTGL-------LKTQSQVVSHICPI---PDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKL--ISQQRPN-VPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
DV+F+LD S + S+++F+ K F ++ ++ + R+ + + ++ +++ ++L + N G A+ KEV+ RK V ++ VL T D +N+ A A L+ V I T+GI SD + ++ + ++++F L LK ++H + L+ C E+ D+ +++D S S D+ + K FL + + F + P K RV + ++D +F Y D+ ++++ + GG T G AL S L +QR N VP +++++T+G++ LE ANRL+ + + ++GI +E N +L+ I+ E+K + D K ++ + IC
Sbjct: 229 DVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKA----AVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSK--DSILEPANRLREEHIRVYAIGI-KEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEIC 611 HSP 5 Score: 59.6918 bits (143), Expect = 5.739e-8 Identity = 52/203 (25.62%), Postives = 92/203 (45.32%), Query Frame = 1
Query: 745 VVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS---QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
+ SHI D G E D+V ++DSS+ + + + K F+ K +S+ + +K RVA+ +SD++ +F + + M+N ++ F GG+ IGKAL+E S R F +++ + E + L+ G IIS+G+ E NLK + + TV +++ ++K VI
Sbjct: 11 ICSHISVNQDSGPE------YADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVI 207
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|A8TX70|CO6A5_HUMAN (Collagen alpha-5(VI) chain OS=Homo sapiens OX=9606 GN=COL6A5 PE=1 SV=1) HSP 1 Score: 108.227 bits (269), Expect = 2.366e-23 Identity = 93/363 (25.62%), Postives = 174/363 (47.93%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKL---VVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQV-ESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK-ILTVETYGDLK 1302
D+ F++DGS SI + FE K+F ++ + + R+ +++ DD E+ F Y+ + + N + L T GKAL+ L+ + M+ + K+ +++ TDG + + P A +L+ + + +GIG+ I EL +A + ++F L +++VV IC EK C + D++ ++DSS SI E++ + K F+ L+ ++G +K ++ ++ FSD+ + +F Y+ ++ + + + T GKAL V + R +++ITDGV R + A L+ K I S+G+ N +L+ IS + + VE + LK
Sbjct: 442 DIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEP---AKRLRAEQITVHAVGIGAANKI-ELQEIAGKEERVSFGQNFDALKSIKNEVVREICA------EKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVR--DPARILRGKDVTIFSVGV-YNANRSQLEEISGDSSLVFHVENFDHLK 791 HSP 2 Score: 100.523 bits (249), Expect = 6.275e-21 Identity = 94/389 (24.16%), Postives = 185/389 (47.56%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT--MRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ----RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELK-MISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQV 1356
C + D++F++D S SI ++F K F K+++T I+ + +I ++F D + F+ Y +++ + + T GKALN + + R +K ++L TDG + P A L+ +V I ++G+ + N ++L ++ L VE+F L + +++ +C + D K TL D+V +LD S SI ++ + N + + ++G ++ + L +SD+ F Y +I ++ GG T KAL+ ++ + + NV +++ITDG ++ + + A L+ KG I ++G+G + N KEL+ M +++ + V+ + LK T+++ +C P+V
Sbjct: 621 GCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDP---ARILRGKDVTIFSVGV-YNANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDC---KRITL--LDVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGVG-KANQKELEGMAGNKNNTIYVDNFDKLKDVF-TLVQERMCTEAPEV 998 HSP 3 Score: 80.4925 bits (197), Expect = 1.776e-14 Identity = 85/358 (23.74%), Postives = 168/358 (46.93%), Query Frame = 1
Query: 319 MTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE-VEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGL--------LKTQSQVVSHICPIPDP-GLEKPCTLE--QQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALR---EVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
+ ++ SN+ I+F+K + EFQ K + + +NT I + E Y + R + V ++ VL T ++ +L++ NV L+I ++ + E+ ++S+ L K Q+++ S I + L+K ++ + D+ ++D S+SI E+ +EQ K F+++ F +GP+K RV ++ +SD+ +F TDY D+ + ++ + G T GKAL ++ + + VP ++++TDG++ R +E A RL+ + + ++GIG N EL+ I+ +++ ++ D KS+K + IC +
Sbjct: 274 LGLMSYSNSAKTISFLK---SSTTQSEFQQQIKNLSIQVGKSNT-----GAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVHDAALNLRLEDVNVFALSIQGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMST--DRVVEPAKRLRAEQITVHAVGIG-AANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEK 620 HSP 4 Score: 56.9954 bits (136), Expect = 3.670e-7 Identity = 45/182 (24.73%), Postives = 85/182 (46.70%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKF-GGATGIGKALREVDSKLIS-----QQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI--GEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTV 1332
D+V ++DSS+ + + + K F+ K +++ + K RVA+ +SDE +F + + M+N +K +F GG+ IGKAL+E S + R P ++++ + E + L+ G IIS+G+ E NLK + + T+ +++ ++K V
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESE--DEVEEASKALQKDGVKIISVGVQKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDV 209
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|Q70UZ7|VWA2_MOUSE (von Willebrand factor A domain-containing protein 2 OS=Mus musculus OX=10090 GN=Vwa2 PE=1 SV=1) HSP 1 Score: 104.76 bits (260), Expect = 1.960e-22 Identity = 81/368 (22.01%), Postives = 161/368 (43.75%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRK----EVEKLVVLFTDGTNN---MYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQ-SQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ---RPNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDL 1299
DVLF+LD S + F AK F K + + ++ R+ +G + + Y + ++ + + T G AL + + + + ++VVL T+ + PA + A +L L +G+GS+I EL ++ + V + L +Q ++ +C P PG C + DLV +LD+S S+ E++ Q ++F+ K F++ P+ +V ++ + ++ FG + ++ + GG G AL ++ K+++ Q RP VP ++++T G + P A +L+ G +++ + +G + ++ D ++ V Y DL
Sbjct: 342 DVLFLLDSSAGTTLGGFRRAKAFVKRFVQAVLREDSRARVGIASYGRNLMVAVPVGEYQHVPDLIRSLDSIPFSGGPTLTGSALLQVAEHGFGSASRTGQDRPRRVVVLLTESRSQDEVSGPAAHARAREL-------LLLGVGSEILQAELVKITGSPKHVMVHTDPQDLFSQIPELQRRLCSQPRPG----CQAQSLDLVFLLDASASVGRENFAQMQSFIRKCTLRFDVNPDVTQVGLVVYGSRVQTAFGLDTHPTRAAVLRAMSQAPYLGGVGSAGTALLHIEDKVMTVQRGARPGVPKAVVMLTGGSGAEDAAVP-----AQKLRGNGISVLVMSVGAVLREAVRRLAGPRDSLIHVAAYTDL 693 HSP 2 Score: 60.4622 bits (145), Expect = 2.150e-8 Identity = 39/153 (25.49%), Postives = 74/153 (48.37%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK 1269
D++ +LD S+SI + +E+ K F + ++ P + RV L F +F + ++ +K ++ GG T G AL+ + + +VP ++I+TDG + P L A +L+ +G + ++G+ EL ++SE K
Sbjct: 51 DILFLLDGSHSIGKGSFERSKRFAIAACDALDISPGRVRVGALQFGSTPHLEFPLDSFSTRQEVKESIKGIVFKGGRTETGLALKRLSRGFPGGRNGSVPQILIIVTDG-KSQGPVALP-AKQLRERGIVVFAVGV-RFPRWDELLTLASEPK 200 HSP 3 Score: 54.299 bits (129), Expect = 2.122e-6 Identity = 40/151 (26.49%), Postives = 71/151 (47.02%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRK-EVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS 687
D+LF+LDGS SI K FE +K+F + S R+ ++FG + F +++ +++ + T G AL L +P R V +++++ TDG + A + A +L+E + + +G+ +EL LAS
Sbjct: 51 DILFLLDGSHSIGKGSFERSKRFAIAACDALDISPGRVRVGALQFGSTPHLEFPLDSFSTRQEVKESIKGIVFKGGRTETGLALK-RLSRGFPGGRNGSVPQILIIVTDGKSQGPVA--LPAKQLRERGIVVFAVGV-RFPRWDELLTLAS 197
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: A0A210PQ58 (Collagen alpha-6(VI) chain OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT09961 PE=4 SV=1) HSP 1 Score: 157.147 bits (396), Expect = 1.930e-37 Identity = 112/418 (26.79%), Postives = 199/418 (47.61%), Query Frame = 1
Query: 121 NLFLAVLPILIGFFTFNVKTDDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT---MRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPI--PDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGAT----GIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE-DKILTVETYGDLKKSLKTVMKTVICG 1341
N F V +L GF+ + D C D++F+LD S S + +F+ F K++ I +I F F TY + + N + +T G+ +N + + + MR +V ++V+ TDG + A K +A ++++ IGIG ++ +EL +AS P L VE+F L Q V + C I P P C Q D+V ILDSS S + ++++ F+ F++N ++GP+ +V +++FSD ++ +F + D++ K++++ G T G+ A R+ RPN +++TDG ++ P L A+RLK G IIS+GIG +++ +E+ I+S+ + TV T+ D ++++ ++ CG
Sbjct: 3 NRFQCVTAVLFGFYISLIVAQGTQD--------CRGQKSDIIFLLDSSTSEGQQNFKHQLDFVKNLTAIYDIGPNNVQIGLASFSSHVHNQFWLNTYHNQASLQNAIGNVPFTFGNTNTGEGINFVTTQGFTSAHGMRNDVPNILVVMTDGQSQDGTNTASMASKAHQAGIEVVAIGIGDGVSTSELQAIASDPKHILTVENFLALASIQHNVAAATCNIATPAPLTALGCKTSQADVVFILDSSGSEGQNNFQKELQFVSNFVNNVQIGPQNIQVGLVTFSDIVKNEFYLNSFNNKLDIMTKLQNVSYRGSTTYTELGLQYA-RQFQFTSRHGARPNATKIAVVLTDGQSHSRPATLSEASRLKQIGVKIISVGIGTQVSQEEINGIASDSNHAFTVATF-DALHTIQSELENTACG 410 HSP 2 Score: 110.538 bits (275), Expect = 7.059e-22 Identity = 102/390 (26.15%), Postives = 183/390 (46.92%), Query Frame = 1
Query: 208 LTA-ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTY-------TKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKAL---REVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKE-LKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
LTA C DV+FILD S S +++F+ +F + + ++ ++ + F D + F ++ TKL+ + + T Y+ G+ A + R K+ V+ TDG ++ PA A +LK+ V+I+++GIG+ ++ E+ +AS + V +F L QS++ + C P + C + D+V ILDSS S +++ + F+ F+ +F +GP + +++FS + R +F Y D+ NK+ ++ G T L R+ R N +I+TDG + + A+ LK G IIS+GIG I+ E L M + + +LTV ++ D ++++ +K C
Sbjct: 220 LTALGCKTSQADVVFILDSSGSEGQNNFQKELQFVSNFVNNVQIGPQNIQVGLVTFSDIVKNEFYLNSFNNKLDIMTKLQNVSYRGSTT---YTELGLQYARQFQFTSRHGA-RPNATKIAVVLTDGQSHSRPATLSEASRLKQIGVKIISVGIGTQVSQEEINGIASDSNHAFTVATFDALHTIQSELENTACGGTIPPVTTTCGSKPADIVFILDSSGSEGGDNFNKQLQFMSNFVKHFSIGPHDVQFGLVTFSIQARNEFYLNTYNSVQDISNKLTNIAYIGSTTHTELGLQYARQFQFSSSHGARQNAQKIAIILTDGQSQSSTDTIHQADLLKQMGVKIISIGIGSYISQTELLSMAADQQHVLTVGSF-DALHTIQSELKESSC 604 HSP 3 Score: 80.4925 bits (197), Expect = 3.674e-12 Identity = 94/398 (23.62%), Postives = 169/398 (42.46%), Query Frame = 1
Query: 199 ITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSST----GIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS-LQVESFTGLLKTQSQVVSHICPI-------PDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGA--TGIG-KALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANI--ISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICG 1341
I +T C D++FILD S S D+F +F + + + + + F A F TY ++ + +TN + S+T G+ A + R+ +K+ ++ TDG + AD LK+ V+I++IGIGS I+ EL +A+ L V SF L QS++ C G C D+V +D+S + ++++ F+ + ++ F P + +++F +F + Y +I ++S GG T IG +R RP V +++IT+G ++ P L L+ + N+ I++GIG +N+ E+ +++ + D +LK + ICG
Sbjct: 413 IPPVTTTCGSKPADIVFILDSSGSEGGDNFNKQLQFMSNFVKHFSIGPHDVQFGLVTFSIQARNEFYLNTYNSVQDISNKLTNIAYIGSTTHTELGLQYARQFQFSSSHGA-RQNAQKIAIILTDGQSQSSTDTIHQADLLKQMGVKIISIGIGSYISQTELLSMAADQQHVLTVGSFDALHTIQSELKESSCGGGAGGTTPASTGTNSACLA---DVVFAIDASGVEGQSNFQKSLAFMSEVVNEFPGVPYNVKFGLVTFGSHASTNFYLSQYNSKTSLIRAIQSTSYIGGTQNTWIGLYVVRHTTLTTAKGARPGVKPYVILITNGKSSSHPSFLRETTSLR-QATNMTGIAIGIGNGVNMDEINSFANDGHHAFHVSNADGLLTLKHTIHDSICG 805
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: K1PNS9 (Cartilage matrix protein (Fragment) OS=Crassostrea gigas OX=29159 GN=CGI_10005977 PE=4 SV=1) HSP 1 Score: 143.665 bits (361), Expect = 3.423e-34 Identity = 105/394 (26.65%), Postives = 189/394 (47.97%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTEN--RIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVY---PTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRL-ASPGLSLQVESFTGLLKTQSQVVSHIC----------------PIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKEL-KMISSEDK----ILTVETYGDLKKSLKTVMKTVIC 1338
D++FI+D S SI +++ +F + + + + +N R+ I F D+ + F YT+ ++L + L T A+ L L+ ++ R+E + ++++ TDG + A A K +E + +L IG+G ++N+ EL + +P QV F L +++ C P + + C + D++ +LDSS+SI D+++ NF+ +S F++GPEK V + +FSD+ R F D+ +D++ V ++ G T G AL+ + R NV ++++TDG++ L A+ LK G NI ++G+G I+ KEL M SS + + V TY D KS++ ++ C
Sbjct: 7 DLVFIIDSSSSIFPNEYVRQLEFLETIAGALDVGHLQNQSRVGVISFSDEPRLEFTLNQYTERSELLSAIRRINYLSGGTNTAAAIQLALETMFNEESGAREEAQDIIIVITDGESFSESATKASAQKARELGIFVLAIGVGVNVNVRELNLITGNPEHVYQVLKFEDLRDITETLINKTCTETFITTSLPAVQKDLPHNESSTFESCDEKPADVLFLLDSSSSIHRNDFKKQLNFVKTIVSAFDIGPEKTTVGVSTFSDDFRLVFNLNDFTTKEDILGAVDNVPYLRGGTDTGFALKNIREYGFRLARSNVAHILIVVTDGLSRDPSDTLLQASLLKEIGINIFAIGVGPFIDKKELISMASSPTEDLNFVFDVSTY-DALKSVENLLAKRTC 399 HSP 2 Score: 70.8626 bits (172), Expect = 2.111e-9 Identity = 52/200 (26.00%), Postives = 99/200 (49.50%), Query Frame = 1
Query: 793 CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKN--RVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ---RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSED----KILTVETYGDLKKSL--KTVMKTVICGNQPQVE 1359
C DLV I+DSS+SI +Y + FL ++G +N RV ++SFSDE R +F Y + ++++ ++ + G T A++ + +++ R I++ITDG + A + + G ++++G+G +N++EL +I+ ++L E D+ ++L KT +T I + P V+
Sbjct: 1 CVENPVDLVFIIDSSSSIFPNEYVRQLEFLETIAGALDVGHLQNQSRVGVISFSDEPRLEFTLNQYTERSELLSAIRRINYLSGGTNTAAAIQLALETMFNEESGAREEAQDIIIVITDGESFSESATKASAQKARELGIFVLAIGVGVNVNVRELNLITGNPEHVYQVLKFEDLRDITETLINKTCTETFITTSLPAVQ 200
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: A0A2T7PE87 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_07148 PE=4 SV=1) HSP 1 Score: 141.354 bits (355), Expect = 2.013e-33 Identity = 99/380 (26.05%), Postives = 195/380 (51.32%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDA--EIVFEFQTYT---KLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLK-TQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFD--FGFTDYYKSDDMINKVKSL-IKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGI---GEEINLKELKMISSE-DKILTVETYGDLKKSLKTVMKTVIC 1338
++ F++D S SI D+F L F +D + + S + R++ + +G+ A + F F YT L + + +V + ++T G +N + P R EV ++V++ TDG + A + +EA +++ IG+G ++ EL R+AS + V S +L+ + ++ C C ++ DL +I+DSS SI E ++ G +F+ F+ +F++ P RV++++F D + D F F Y S D+++K++++ + G +T G A+ + + + RP ++ITDG + ++ + E A + + KG + ++GI GEE++ EL I+ + ++L + Y L +++ + T+ C
Sbjct: 38 EISFVVDASASIWPDNFTLGLNFIEDFVGLFDISPSAVRVSLVTYGEVAYGDDSFGFNAYTNKDSLVKAISEVPYRSGIKTNTSGG--INFMMDNHKPEARPEVRQVVIVLTDGNSQEPDLTAQAAKRAREAGLEVFAIGVGQSVSAEELHRIASDNRHVFVVSGYKMLEDIKKRLAYEAC--------DACQMDPVDLSLIIDSSQSIGESNFTVGMDFVQNFVKSFQVNPHTVRVSVVTFGDVVYTDDAFDFDTYDNSKDLLDKLQAIKWRHGTSTNTGAAIEYMRDVQMVKSRPTAAHVCIVITDGQSQLWMKTKEEAKKAREKGIVMYAVGIGKLGEELSEDELLGIAGDRSRMLIADNYAKL-NTIREQLTTITC 406
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: G3NF26 (Collagen, type VI, alpha 4a OS=Gasterosteus aculeatus OX=69293 PE=4 SV=1) HSP 1 Score: 144.821 bits (364), Expect = 4.975e-33 Identity = 105/380 (27.63%), Postives = 186/380 (48.95%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNL---TLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQP 1350
D+ F++D S SI DF+ K F + + R+ +K+ D A++ F+ Y+ K + V R + T G+AL T +V + +V+ TDG +++ AP A+KL+ V + IG+ + ++ EL +A P + V +F L + +++ IC PD C +Q DLV ++D S SIS DY K F +S+F+L RV + FS + +F +Y + + + + GG T IG AL + D+ ++ + N+++ITDG + E A+RL+ G + ++GIG+ +L+ L++ + D++ TV+++G L+K + V+ T IC ++P
Sbjct: 1374 DIFFLIDHSGSIYPKDFQDMKTFIIGFLHTFRLGPQHVRVGVVKYADSADLEFDLTAYSDAKTLEKAVEGIRQIGGGTETGRALEFMSPQFDRAMATRGHKVPEYLVVITDGKSSDKVKAP---AEKLRSKGVIVYAIGV-KNADVVELGEIAGDPKRTFFVNNFDALRPIKDDIITDICS-PDV-----CEKQQADLVFLIDQSGSISANDYTTMKQFTTALVSSFKLSENLVRVGLAQFSSTFQNEFYLNQFYSDQAVTKHILDMKQIGGGTNIGLALHSIIDHFDASRGGRRSARISQNLVLITDGESQ--DDVEEAADRLRALGIEVFAIGIGDVHDLELLQITGTPDRLFTVQSFGSLEKIKQKVVDT-ICKSRP 1740 HSP 2 Score: 103.219 bits (256), Expect = 2.827e-19 Identity = 94/403 (23.33%), Postives = 185/403 (45.91%), Query Frame = 1
Query: 202 TSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTDG--TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS---PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTL---------EQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVD---SKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
T LT C + D++FI+D S SI +F L + F +++ ++ + R+ + + + A ++ ++L + T G AL L+EV+ R K ++++ V+ TDG +N+ A A L+ + + +G+ D N +L +AS V+SF L + + +C + L + ++ ++ D+ ++D S SI +D++ K F++ FL F LGP+ RV ++ ++D +F T Y + + V+ + + GG T G+AL + + ++ + VP +++ITDG ++ P E +L+ KG + ++G+ + ++ EL I+ + K D + +K + T IC
Sbjct: 1159 TVLTGDCKAAKLADIVFIVDESGSIGSPNFHLVRTFLHSLVSGLEVGTSRVRVGIVTYNERATAQVYLNSFNDKSELLKFIKMLPYQGGGTYTGAALEFALREVFIPQRGGRRAKGIQQVAVVITDGESQDNVSTA----AANLRREGITVYALGV-KDANDAQLVAMASYPPHKHKFTVDSFAKLKSVEQSLQKLLC---NNILRQAVSINTRRTGIKTDEADIFFLIDHSGSIYPKDFQDMKTFIIGFLHTFRLGPQHVRVGVVKYADSADLEFDLTAYSDAKTLEKAVEGIRQIGGGTETGRALEFMSPQFDRAMATRGHKVPEYLVVITDGKSSDKVKAPAE---KLRSKGVIVYAIGV-KNADVVELGEIAGDPKRTFFVNNFDALRPIKDDIITDIC 1549 HSP 3 Score: 100.908 bits (250), Expect = 1.663e-18 Identity = 102/407 (25.06%), Postives = 185/407 (45.45%), Query Frame = 1
Query: 199 ITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVY-----PTMRKEVEKLVVLFTDG--TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVES---FTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQ-------DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKAL---------REVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS---EDKILTVETYGDLKKSLKTVMKTV 1332
+ S +A +D++F++DGS SI ++F+ ++F ++ RI +++ F T+ K +L V++ L T G +L+ LKE + + V ++ V+ TDG +N+ +A LK + + IGI D + +L +AS S V S F L +V +C + + L Q+ D+V ++D S+SI ++++ + FL +S ++GP+K RV + FSDE +F ++ ++ V G T GKA+ E S++ QR VP ++ITDG + +E A RL+ G + ++G+G N KEL+ I++ E + ++ Y L++ +++TV
Sbjct: 575 LHSCSAEVHLSRKDIVFMVDGSWSIGSENFKQIRRFLHTLVDSFDVRPEHVRIGLVQYSTAPRTEFLLNTFRSKKDILRYVSSLPYLGGGTQTGLSLDFMLKEHFVEAAGSRGAESVPQIAVVITDGKSQDNVES----HAQDLKREGIVLYVIGI-KDADEEQLREIASEPHSQHVYSVSDFAALQGISQSIVQTLCTTVEEVKRQLLQLSQECAKATVADIVFLVDGSSSIGISNFQEIRQFLRSVVSGLDIGPDKVRVGLAQFSDETTQEFLLKEHMDKKSLLASVDIFPYRTGGTETGKAIDFLLNNYFTEEAGSRV--NQR--VPQIAVVITDG--DSTDDVMEPAQRLRQLGVIVFAIGVGAA-NRKELESIANRPPERFLSSINNYQALQRLTVGLLQTV 969 HSP 4 Score: 72.4034 bits (176), Expect = 1.913e-9 Identity = 77/385 (20.00%), Postives = 174/385 (45.19%), Query Frame = 1
Query: 196 QITSLTAACT-FIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTL-----KEVYPTMRKEVEKLVVLFTDGTNN---MYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS--PGLSL-QVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQ-----DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSL----IKFGGATGIGKALREVDSKLISQQ---RPNVPF-NILIITDGVNNMFPRPLEYANRL-KMKGANIISLGIGEEINLKELKMISSEDKI 1272
Q+ L+ C + D++F++DGS SI +F+ ++F + +++ + + R+ +F D+ F + + K +L V T GKA++ L +E + + V ++ V+ TDG + M P A +L++ V + IG+G+ N EL +A+ P L + ++ L + ++ +C ++E Q ++ ++DS +S++D++Q + L + ++ +G +R+ + + + R +F + D++N +K ++ +G ALR + + + R + + L++ G + P+ +RL K G ++ + + E +L E++++++ I
Sbjct: 776 QLLQLSQECAKATVADIVFLVDGSSSIGISNFQEIRQFLRSVVSGLDIGPDKVRVGLAQFSDETTQEFLLKEHMDKKSLLASVDIFPYRTGGTETGKAIDFLLNNYFTEEAGSRVNQRVPQIAVVITDGDSTDDVMEP-----AQRLRQLGVIVFAIGVGA-ANRKELESIANRPPERFLSSINNYQALQRLTVGLLQTVC----------ISVEDQRQGEKNIFFLVDS--GMSQKDFQQIRTLLTRLVNQLNIGGSSHRLGLAQYGQDTRVEFLLNAFQNKQDILNGIKQFGQQKLQTNEPRNLGGALRYAATNFFTSEAGSRADQGYRQFLVVISGKESA--DPVHRESRLIKSSGVTVVGMSLSE--SLMEMRIVATAPYI 1138 HSP 5 Score: 62.003 bits (149), Expect = 2.959e-6 Identity = 69/316 (21.84%), Postives = 135/316 (42.72%), Query Frame = 1
Query: 163 TFNVKTDDQND-QITSLTAACTFIIR--DVLFILDGSQSISKDDFELAKKFTKDMMTIIKN---SNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK---------EVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKAL 1062
T + DDQ + T C R D+ F+LD + +I + F + F KD + +IKN + RI ++G+D ++ F T+ Q L + R L + + L + LK E + +V+ + G + + + + K E I G+ + + + +ASPG FT + Q+ + + + + + C + D+V ++D S SI+ +++ ++FL +S ++GP K +V +++++DE + + +++ + L GG T G AL
Sbjct: 179 TVCISVDDQRQGEATDWRKQCVHTDRFGDIFFLLDSNIAIGQ--FNI---FKKDFIRLIKNLDVGASAYRIGLAQYGEDPKVEFNLSTHQTKPQTLAAIERFR-LRPPSNRQRNLGIALKYASDHFFTREAGGRAHLGAPQSLVVVSGGDSTDFVSDGAHLLKSSE----ITVYGMSAGASQTAIESVASPG-------FTFMSALVYQLKESLTTVKNESISEDCKAVNMADIVFVVDESGSITTPNFQLVRDFLRSVVSGLKVGPTKVQVGIVTYNDESTPRVYLNSFDEKAEILQFINILPYNGGGTKTGAAL 477
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: A0A4W6DQR4 (Uncharacterized protein OS=Lates calcarifer OX=8187 PE=4 SV=1) HSP 1 Score: 139.813 bits (351), Expect = 5.849e-33 Identity = 96/363 (26.45%), Postives = 189/363 (52.07%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPD--PGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKK 1305
D+ F++D S SI +DF + KKF + + R+ +K+ DD + F+ Y+ +++ V T ++ T GKAL+ + +++ + R+ V +++ TDG ++ + A+KL+ ++I IG+ D N EL +A P + QV +F L + V+ IC IPD L + E+ D+V +LD S+SI+ E+Y NF + + ++ + RV F+ +F +DY + D+++++ L GG T +G+AL+ + D+ S++ +P N+++I+DG + E A+ L+ G + ++ +G+ +L+ L++ + +++ TV+ + +KK
Sbjct: 22 DIFFLMDASGSIVDEDFSVMKKFIIEFIHAFNIGPKHIRMGLVKYSDDPVLEFDLAAYSDARKLERAVEYTEHIRGRTFTGKALS-KMGDLFVSRRQNVPTYLIVITDGKSDESDPVRIPAEKLRAQGIKIYAIGV-KDSNEAELVEIAGDPKRTFQVTNFDALKSIKEDVIRDIC-IPDGKDSLSRQ-NCEEADVVFLLDRSSSITPENYTIMLNFTKELVKTLDVRKDFIRVGCAQFASAPHHEFYLSDYTEKKDVMSRIDRLSYIGGNTYLGEALKHIKDYFDASHGSRRSVGIPQNLVLISDG--DSHDDVEEAADSLRALGIVVFAIAVGDVHDLQLLQIAGTPERLFTVQNFKVVKK 378 HSP 2 Score: 77.411 bits (189), Expect = 1.753e-11 Identity = 55/201 (27.36%), Postives = 99/201 (49.25%), Query Frame = 1
Query: 736 QSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
+ Q H+C D + D+ ++D+S SI +ED+ K F+++F+ F +GP+ R+ ++ +SD+ +F Y + + V+ G T GKAL ++ +S +R NVP +++ITDG ++ A +L+ +G I ++G+ + N EL I+ + K T D KS+K + IC
Sbjct: 7 RCQCCRHLCERKD----------EADIFFLMDASGSIVDEDFSVMKKFIIEFIHAFNIGPKHIRMGLVKYSDDPVLEFDLAAYSDARKLERAVEYTEHIRGRTFTGKALSKMGDLFVS-RRQNVPTYLIVITDGKSDESDPVRIPAEKLRAQGIKIYAIGVKDS-NEAELVEIAGDPKRTFQVTNFDALKSIKEDVIRDIC 195
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303]) HSP 1 Score: 112.849 bits (281), Expect = 7.939e-26 Identity = 98/390 (25.13%), Postives = 183/390 (46.92%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK-----EVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVES---FTGLLKTQSQVVSHICPIPDPGLEKP------CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLIS-----QQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK---ILTVETYGDLKKSLKTVMKTV 1332
+ D++F++DGS SI ++F+ ++F ++ + RI +++ F Y +++L ++N T G L+ L+ E +K ++ V+ TDG + PY A +LK+ + + IGI D ++++L +AS + S FT L ++ +C + ++ C D+V ++D S+SI + D+ + K FL F+ E+ + RVA++ FS+ D DY ++++ KV L+ G+T GKAL + + + Q R NVP ++ITDG + P A L+ +G I ++G+G E+N+ EL+ I+++ +++ Y L + +M TV
Sbjct: 32 LADIVFLVDGSWSIGTENFQRMREFLFTLVDSFDVGLEKVRIGLVQYSTTVHTEFLLNGYNSKQEILDYISNLPYRGGGTKTGLGLDFLLRYHFKEEAGSRAKKGTPQIAVVITDGQSQDNVEPY--AQELKQKGIILYAIGI-KDADMDQLKEIASQPHDQHIYSVSDFTALQGISQSFITVLCSTVEEEAKRQVTQVPDCRANLADIVFLVDGSSSIGDADFLRMKRFLHAFIQGLEISADSIRVAVVQFSNNPHEDIPLGDYADKNELLEKVDRLVYHRGSTETGKALNFIKDRYFTQAGRIQARANVPRIAVVITDGDSTDDMKTP---AQELRREGVLIFTIGVG-EVNMAELQSIANKPHQRFVISFVNYEALVNAASNMMNTV 414 HSP 2 Score: 110.538 bits (275), Expect = 3.593e-25 Identity = 106/388 (27.32%), Postives = 175/388 (45.10%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM--RKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQ-QDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKAL----REVDSKLISQQRPNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK-ILTVETYGDLKKSLKTVMKTVICGN 1344
AC ++ D++F++DGS SI + F KKF + ++ +K ++ ++FG + F+ +Y QM+ V+ T + ++ IGKALN K PT R+ K ++L T + + AP A L++ V I +IGIG ++ SP + L ++ IC D C Q D+V ++D S SI+EED+++ K F+ ++N E+G R + +S+ F +Y ++ N + L G T AL R + VP + +ITDG + P P A +L G N+ +G+GE EL I+S+ K + V+ Y L K+L + V+C N
Sbjct: 1020 ACKQMMADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILITGAESKDDVNAP---AKALRDKGVTIYSIGIGEAKSLQLRNISGSPKQVYMERNLDALDLLDENLLLKICNSAD-----DCQKTQVADVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYTASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEATDHWDLPSP---AGKLHALGINVFGIGVGEA-KEDELMTIASDPKNVFKVDNYEAL-KALHQNISKVLCNN 1394 HSP 3 Score: 102.834 bits (255), Expect = 1.099e-22 Identity = 94/384 (24.48%), Postives = 190/384 (49.48%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNT-ENRIAF--IKFGDDAEIVFEFQTYTKLKQMLLDVTN----TRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMY---PAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVD----SKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETYGDLKKSLKTVMKTVI 1335
+ DV+F++DGS SI+++DF+ K F M ++ N+ EN + F I + + E+ F + + +++ ++ T + Y+++ + ++ K V +L+ + TDG + P+P A KL + + IG+G + +EL +AS P +V+++ L + +C P C+ DLV+++D S SI + + NF+++F+ + + + R+ + FS + +F ++ + + +K + + T IG AL +VD S S+ + N+L+ITDG +N +RL+ + N+ +GIG +I++K+++ I+ S+D++ ++++ LK L T + VI
Sbjct: 1212 VADVVFLVDGSTSIAEEDFDKMKIF---MNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYTASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEATDHWDLPSP---AGKLHALGINVFGIGVG-EAKEDELMTIASDPKNVFKVDNYEALKALHQNISKVLCNNSKP----ECSKVAADLVILIDGSESIEDAPWNTMINFMMRFVDSLRVREDLFRIGVAQFSTVYKKEFYLNEHMEPAAIKKAIKDIKQMKDGTNIGNALYQVDEFFQSAAGSRINSGISQNLLLITDGKSN--DNIYNATDRLRARNINMFVIGIG-QISMKQIEYIAGSQDRVFVLDSFDALK--LNTTILEVI 1579 HSP 4 Score: 99.3673 bits (246), Expect = 1.512e-21 Identity = 97/404 (24.01%), Postives = 186/404 (46.04%), Query Frame = 1
Query: 199 ITSLTAAC-TFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP-----TMRKEVEKLVVLFTDGTNNMYPAPYVY-ADKLKEANVQILTIGIGSDININELTRLAS---PGLSLQVESFTGLLKTQSQVVSHIC---------PIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQR---PNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
+ S++ C + + D++FI+D S +IS +F L + F ++ + + RI + +G++ + F +T+ +L + KAL ++V+ + V ++ + T+G + P A KL+ + V++ +GI N+ +L ++AS V+SF L + + +C I +++ C E+ D+ ++D S SI D++ K F+++ L +F +GP++ RV ++ F +F T Y + V ++++ GG T G+AL + I ++ V +++ITDG + +P A LK +G +I ++G+ + + L+M SS K+ VE Y L K LK + T IC
Sbjct: 621 VISVSGDCKSAQVADIVFIVDASDAISDSNFNLVRDFLHRLIKGLDVGSDSMRIGMVLYGNEPKAEFYLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEGES---PDDVTSEASKLRRSGVRVYALGIQHK-NVTQLMQIASYPPRKYVYNVDSFAKLTIMEQFLRKTLCNNVMRRTVAKINQFNIKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFMRPYFIEARKTRDTRVREILIVITDGESTDEVKQP---AAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSL-KPLKDEIVTDIC 1016 HSP 5 Score: 98.5969 bits (244), Expect = 3.377e-21 Identity = 94/360 (26.11%), Postives = 154/360 (42.78%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLT---LKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIIT-----DGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVE 1284
D+ F++D S SI DF+ KKF +++ + R+ +KF + F Y + V+N + T G+AL E T V +++++ TDG + + P A LK V I +G+ L +SP VE++ L + ++V+ IC E C D++ ++D S SI E + + K F+ +S ++G + +V ++ F R +F YY M+ V + IGKAL E+ + R N P +++IT D VN A L+ KG I S+GIGE +L +L+ IS K + +E
Sbjct: 841 DIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFMRPYFIEARKTRDTRVREILIVITDGESTDEVKQP---AAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDICS------EDACKQMMADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILITGAESKDDVN-------APAKALRDKGVTIYSIGIGEAKSL-QLRNISGSPKQVYME 1183 HSP 6 Score: 92.8189 bits (229), Expect = 2.115e-19 Identity = 101/404 (25.00%), Postives = 185/404 (45.79%), Query Frame = 1
Query: 76 KKHDSH*EIKS*N*MNLFLAVLPILIGFFTFNVKT--DDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM------RKEVEKLVVLFTDG--TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFT---GLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSL-IKFGGATGIGKALREVDSKLI---SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINL 1236
+ HD H I S ++ F A+ I F T T ++ Q+T + C + D++F++DGS SI DF K+F + ++ S R+A ++F ++ Y ++L V ST GKALN +K+ Y T R V ++ V+ TDG T++M P A +L+ V I TIG+G ++N+ EL +A+ V SF L+ S +++ +C I ++ + D+ V++DSS+ + + FL++ + ++G + NR+A++ F E+ +F Y + I ++ ++ G + +G+A+ V ++L+ S R F ++ G L LK +G I+ + + ++L
Sbjct: 186 QPHDQH--IYS---VSDFTALQGISQSFITVLCSTVEEEAKRQVTQV-PDCRANLADIVFLVDGSSSIGDADFLRMKRFLHAFIQGLEISADSIRVAVVQFSNNPHEDIPLGDYADKNELLEKVDRLVYHRGSTETGKALNF-IKDRYFTQAGRIQARANVPRIAVVITDGDSTDDM-KTP---AQELRREGVLIFTIGVG-EVNMAELQSIANKPHQRFVISFVNYEALVNAASNMMNTVC-ISMEDKQRALAPKYSDVFVLVDSSS----QQIANIRKFLIQLATQLDVGAKVNRMALIQFGGEVNVEFLLNAYNTRREAIALIQKFQLRSSGRSNLGQAMDYVRTRLLNSESGSRIAQGFKQYLLVVGKGETDTAVLRATRALKAEGVKIVEVDTSKRVDL 572
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303]) HSP 1 Score: 109.768 bits (273), Expect = 7.135e-25 Identity = 106/387 (27.39%), Postives = 176/387 (45.48%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM--RKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKAL----REVDSKLISQQRPNVPFNILIITDGV---NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK-ILTVETYGDLKKSLKTVMKTVICGN 1344
AC ++ D++F++DGS SI + F KKF + ++ +K ++ ++FG + F+ +Y QM+ V+ T + ++ IGKALN K PT R+ K ++L T + + AP A L++ V I +IGIG ++ SP + L ++ IC D G K + D+V ++D S SI+EED+++ K F+ ++N E+G R + +S+ F +Y ++ N + L G T AL R + VP + +ITDG + P P A +L G N+ +G+GE EL I+S+ K + V+ Y L K+L + V+C N
Sbjct: 780 ACKQMMADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILITGAESKDDVNAP---AKALRDKGVTIYSIGIGEAKSLQLRNISGSPKQVYMERNLDALDLLDENLLLKICNSAD-GESKT---QVADVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYTASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEATDHWDLPSP---AGKLHALGINVFGIGVGEA-KEDELMTIASDPKNVFKVDNYEAL-KALHQNISKVLCNN 1154 HSP 2 Score: 103.99 bits (258), Expect = 5.560e-23 Identity = 92/385 (23.90%), Postives = 192/385 (49.87%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNT-ENRIAF--IKFGDDAEIVFEFQTYTKLKQMLLDVTN----TRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMY---PAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVD----SKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS-SEDKILTVETYGDLKKSLKTVMKTVIC 1338
+ DV+F++DGS SI+++DF+ K F M ++ N+ EN + F I + + E+ F + + +++ ++ T + Y+++ + ++ K V +L+ + TDG + P+P A KL + + IG+G + +EL +AS P +V+++ L + +C P C+ DLV+++D S SI + + NF+++F+ + + + R+ + FS + +F ++ + + +K + + T IG AL +VD S S+ + N+L+ITDG +N +RL+ + N+ +GIG +I++K+++ I+ S+D++ ++++ LK + T+++ + C
Sbjct: 972 VADVVFLVDGSTSIAEEDFDKMKIF---MNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYTASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEATDHWDLPSP---AGKLHALGINVFGIGVG-EAKEDELMTIASDPKNVFKVDNYEALKALHQNISKVLCNNSKP----ECSKVAADLVILIDGSESIEDAPWNTMINFMMRFVDSLRVREDLFRIGVAQFSTVYKKEFYLNEHMEPAAIKKAIKDIKQMKDGTNIGNALYQVDEFFQSAAGSRINSGISQNLLLITDGKSN--DNIYNATDRLRARNINMFVIGIG-QISMKQIEYIAGSQDRVFVLDSFDALKLN-TTILEVINC 1341 HSP 3 Score: 99.7525 bits (247), Expect = 1.158e-21 Identity = 97/404 (24.01%), Postives = 186/404 (46.04%), Query Frame = 1
Query: 199 ITSLTAAC-TFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP-----TMRKEVEKLVVLFTDGTNNMYPAPYVY-ADKLKEANVQILTIGIGSDININELTRLAS---PGLSLQVESFTGLLKTQSQVVSHIC---------PIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQR---PNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
+ S++ C + + D++FI+D S +IS +F L + F ++ + + RI + +G++ + F +T+ +L + KAL ++V+ + V ++ + T+G + P A KL+ + V++ +GI N+ +L ++AS V+SF L + + +C I +++ C E+ D+ ++D S SI D++ K F+++ L +F +GP++ RV ++ F +F T Y + V ++++ GG T G+AL + I ++ V +++ITDG + +P A LK +G +I ++G+ + + L+M SS K+ VE Y L K LK + T IC
Sbjct: 381 VISVSGDCKSAQVADIVFIVDASDAISDSNFNLVRDFLHRLIKGLDVGSDSMRIGMVLYGNEPKAEFYLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEGES---PDDVTSEASKLRRSGVRVYALGIQHK-NVTQLMQIASYPPRKYVYNVDSFAKLTIMEQFLRKTLCNNVMRRTVAKINQFNIKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFMRPYFIEARKTRDTRVREILIVITDGESTDEVKQP---AAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSL-KPLKDEIVTDIC 776 HSP 4 Score: 98.9821 bits (245), Expect = 2.069e-21 Identity = 94/360 (26.11%), Postives = 154/360 (42.78%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLT---LKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIIT-----DGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVE 1284
D+ F++D S SI DF+ KKF +++ + R+ +KF + F Y + V+N + T G+AL E T V +++++ TDG + + P A LK V I +G+ L +SP VE++ L + ++V+ IC E C D++ ++D S SI E + + K F+ +S ++G + +V ++ F R +F YY M+ V + IGKAL E+ + R N P +++IT D VN A L+ KG I S+GIGE +L +L+ IS K + +E
Sbjct: 601 DIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFMRPYFIEARKTRDTRVREILIVITDGESTDEVKQP---AAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDICS------EDACKQMMADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILITGAESKDDVN-------APAKALRDKGVTIYSIGIGEAKSL-QLRNISGSPKQVYME 943 HSP 5 Score: 90.1225 bits (222), Expect = 1.338e-18 Identity = 87/346 (25.14%), Postives = 160/346 (46.24%), Query Frame = 1
Query: 220 CTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM------RKEVEKLVVLFTDG--TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFT---GLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSL-IKFGGATGIGKALREVDSKLI---SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISL 1212
C + D++F++DGS SI DF K+F + ++ S R+A ++F ++ Y ++L V ST GKALN +K+ Y T R V ++ V+ TDG T++M P A +L+ V I TIG+G ++N+ EL +A+ V SF L+ S +++ +C I ++ + D+ V++DSS+ + + FL++ + ++G + NR+A++ F E+ +F Y + I ++ ++ G + +G+A+ V ++L+ S R F ++ G L LK +G I+ +
Sbjct: 27 CRANLADIVFLVDGSSSIGDADFLRMKRFLHAFIQGLEISADSIRVAVVQFSNNPHEDIPLGDYADKNELLEKVDRLVYHRGSTETGKALNF-IKDRYFTQAGRIQARANVPRIAVVITDGDSTDDM-KTP---AQELRREGVLIFTIGVG-EVNMAELQSIANKPHQRFVISFVNYEALVNAASNMMNTVC-ISMEDKQRALAPKYSDVFVLVDSSS----QQIANIRKFLIQLATQLDVGAKVNRMALIQFGGEVNVEFLLNAYNTRREAIALIQKFQLRSSGRSNLGQAMDYVRTRLLNSESGSRIAQGFKQYLLVVGKGETDTAVLRATRALKAEGVKIVEV 361 HSP 6 Score: 83.1889 bits (204), Expect = 2.629e-16 Identity = 57/200 (28.50%), Postives = 100/200 (50.00%), Query Frame = 1
Query: 760 CPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLIS-----QQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDK---ILTVETYGDLKKSLKTVMKTV 1332
C + + C D+V ++D S+SI + D+ + K FL F+ E+ + RVA++ FS+ D DY ++++ KV L+ G+T GKAL + + + Q R NVP ++ITDG + P A L+ +G I ++G+G E+N+ EL+ I+++ +++ Y L + +M TV
Sbjct: 16 CYLSSSAQKTDCRANLADIVFLVDGSSSIGDADFLRMKRFLHAFIQGLEISADSIRVAVVQFSNNPHEDIPLGDYADKNELLEKVDRLVYHRGSTETGKALNFIKDRYFTQAGRIQARANVPRIAVVITDGDSTDDMKTP---AQELRREGVLIFTIGVG-EVNMAELQSIANKPHQRFVISFVNYEALVNAASNMMNTV 211
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: si:ch211-62a1.3 (si:ch211-62a1.3 [Source:ZFIN;Acc:ZDB-GENE-061207-41]) HSP 1 Score: 103.219 bits (256), Expect = 8.988e-23 Identity = 93/361 (25.76%), Postives = 166/361 (45.98%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSS-TGIGKALNLTLKEVY-----PTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHIC----------PIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS 1260
D++F++DGS SI +F+ ++F ++T + + RI +++ D F TY KQ +LD + T G+ L L+ + +K V ++ V+ TDG + PY A++L+E ++I IGI D + L +A+ V F L V IC +P+ C D+V+++DSS++I E + + ++FL F+ + ++ K ++ + +SD +F +Y D++ K+ S+ G G AL + SQ R NVP ++ITDGV++ E A +L+M+G + G E N+ +L+ I++
Sbjct: 34 DIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYED-KQEILDFIKVLPYKTGGTNTGEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITDGNSQDEVEPY--AEELREKGIKIYAIGI-KDADEKLLKEIATQPYDQHLFSVSDFEALQGIMLNVTQEICTTVEREGEINSVPE------CNEFAAADIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSSDAVE--EPAQKLRMQGVITFVVKTG-EANILQLRAIAN 381 HSP 2 Score: 102.064 bits (253), Expect = 2.344e-22 Identity = 94/396 (23.74%), Postives = 189/396 (47.73%), Query Frame = 1
Query: 205 SLTAACTFIIR-DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTY-------TKLKQMLLDVTNTR---NLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPY-VYADKLKEANVQILTIGIGSDININELTRLA-SPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDGVNN---MFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
++ C F D+ F+LD S SI+ DDF+ K F + + + + + RI +KF D A IVF TY +K + ++ TR LY A+ K+ T +++V +L+++ TDG + P V A++L++ NV I +G+ + + EL ++ SP + V+++ L +++++ IC + C D+V ++D ++++ D++Q K+ + + + + R+A++ +S + +F ++Y D + ++ ++ + G T G+AL EV + RPNV ++++TD + + P A L+ K N+ ++G+G + L + S + +G L + L + + +IC
Sbjct: 809 TIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLGLY-------AMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGV-KNADTAELEDMSGSPKRTFYVQNYDALKLIKTKILKEICSF------EACNDLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQLKGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDSSSKDDVVAP-----AMALREKNVNVYAVGMGHASQSELLDISGSYETAYLENPFGSL-QVLGSELVFMIC 1184 HSP 3 Score: 97.8265 bits (242), Expect = 5.106e-21 Identity = 98/390 (25.13%), Postives = 176/390 (45.13%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE-----VEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICP------IP---DPGLEKPCTL-EQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDS--KLISQQRPNVPFNILI-ITDGVNNMFPRPLEY-ANRLKMKGANIISLGIGEEINLKELK-MISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
+ DV+FI+D S ++ ++F+L + F ++ ++ + + R+A + + D+ T++ ++L V G+AL V+ R V+++ V+ TDG +++ AP A L+ + V + +GI S N+ E+ V +FT L V IC IP + ++K C + D+ +LD S SI+ +D++ K F+++FL F++GP++ R+ ++ F+D F Y + VK L GG T L + K Q R +LI ITDG + P++ A L+ + I ++G+ + + EL+ M S + V+ Y D K +KT + IC
Sbjct: 615 VADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKSSDDVSAP---AADLRRSGVTVFAVGIKSFDNLKEMASYPQDKFVFSVGNFTKLNALSGVVTKRICRDISSVFIPLLKNITIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLGLYAMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGV-KNADTAELEDMSGSPKRTFYVQNY-DALKLIKTKILKEIC 999 HSP 4 Score: 90.1225 bits (222), Expect = 1.649e-18 Identity = 90/376 (23.94%), Postives = 168/376 (44.68%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTL---KEVYPTMR-KEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPG-LSLQVESFTGLLKTQSQVVSHICPIPDPGLEKP-CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
D++F++DG+ +I+K+ F+ K F + ++T + R + + D A+ VF Y ++L ++ R + +AL TL Y R + V +++ L T+G + + L + V + +G+ + EL R+A + V++++ L + +C L KP C E DLV+++D S S+S D+ K+ L+ + E+ ++ RV++ F D F Y + +++L + +T ALR + + + S+ V N+L+ITDG L L+ K I ++GIG+ IN L I+ + + +E++ L LKT V+
Sbjct: 1195 DIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFTLNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEGPVADSFSLADWPANLAASEVNMFAVGV-AGAREAELRRIAGNNQRAFYVDTYSQLETLCKPITQQLC-----NLTKPVCEKEVADLVILIDGSESVSALDWGTLKSSLINLVRKLEIAQDRWRVSVAQFGDAAPDRFYLNKYNNLAGVEQGIRALSQRRQSTNTWTALRFLQDYFEPEHGSRMNARVSQNLLLITDG-RATDKEDLGVLASLRAKNVEIFAVGIGKNINRHALLKIAGSKERVFLESFESL--PLKTTTAKVL 1561 HSP 5 Score: 82.8037 bits (203), Expect = 3.304e-16 Identity = 95/400 (23.75%), Postives = 187/400 (46.75%), Query Frame = 1
Query: 199 ITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLY----SSTGIGKALNLTLKEVY-PTMRKEV----EKLVVLFTDGT---NNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPG-LEKPCTL-EQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPN-----VPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
I S + A + D+ F++D + S+ + + + F ++ + NRI +F ++ E F F K K + V + RNL IG A+N + P + ++ +++ T G+ + + P+ ++K+ V ++ +G+G +++EL +ASP LS +++S T + Q + + P+ + + C L E D+V I+D S ++ E+++ +NFL +S ++G +K RVA++ +SDE R D + +++ VK L G G+AL+ + +++R + V +++TDG ++ P A L+ G + ++GI NLKE+ + + +V + L +L V+ IC
Sbjct: 412 IDSQSQAFSLKFADIFFLVD--SAASRQESQQIRNFISRLVNQLNVGKNTNRIGLAQFSENVEEEFLFDN-DKTKNEI--VASIRNLQLKPKGLRRIGNAINHARTRFFTPAAGSRIAEGFKQFLLVATAGSSVDDVVQPS-----RQIKKEPVNLIAVGVGRP-DMDELKDIASPTLSYKLDSRT--MPVLPQQIKAVIDSPEGHKVTEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKSSDDVSAP---AADLRRSGVTVFAVGIKSFDNLKEMASYPQDKFVFSVGNFTKL-NALSGVVTKRIC 794 HSP 6 Score: 71.633 bits (174), Expect = 1.213e-12 Identity = 84/397 (21.16%), Postives = 179/397 (45.09%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK--EVYPTMRKEVEKLVVLFTDGTN-NMYPAPYVYADKLKEANVQILTIGIGS-------DININ-ELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQVE 1359
AC ++ DV+F++DG+ ++ DF+ AK + + R+A +++ + ++ F + ++ + + L T G+AL + E R V + +V+ TD ++ + AP A L+E NV + +G+G DI+ + E L +P SLQV S++V IC + ++P E D++ ++D + +I++E ++ K F+ ++ E+G ++ R ++ ++D + F YY +++ + L GG +AL+ + ++ +VP + +IT+G V + F ++ L N+ ++G+ + ++ + + V+TY L+ K + + + +P E
Sbjct: 1003 ACNDLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQLKGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDSSSKDDVVAP---AMALREKNVNVYAVGMGHASQSELLDISGSYETAYLENPFGSLQV--------LGSELVFMIC---NTECKRP---ELIDIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFTLNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEGPVADSFSL-ADWPANLAASEVNMFAVGVAGAREAELRRIAGNNQRAFYVDTYSQLETLCKPITQQLCNLTKPVCE 1381 HSP 7 Score: 58.9214 bits (141), Expect = 1.028e-8 Identity = 54/196 (27.55%), Postives = 90/196 (45.92%), Query Frame = 1
Query: 196 QITSLT-AACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP----TMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGL-LK-TQSQVVSHIC 762
Q+ +LT C + D++ ++DGS+S+S D+ K +++ ++ + R++ +FGD A F Y L + + ST AL P M V + ++L TDG V A L+ NV+I +GIG +IN + L ++A + +ESF L LK T ++V+ IC
Sbjct: 1371 QLCNLTKPVCEKEVADLVILIDGSESVSALDWGTLKSSLINLVRKLEIAQDRWRVSVAQFGDAAPDRFYLNKYNNLAGVEQGIRALSQRRQSTNTWTALRFLQDYFEPEHGSRMNARVSQNLLLITDGRATDKEDLGVLA-SLRAKNVEIFAVGIGKNINRHALLKIAGSKERVFLESFESLPLKTTTAKVLQAIC 1565
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: si:ch211-62a1.3 (si:ch211-62a1.3 [Source:ZFIN;Acc:ZDB-GENE-061207-41]) HSP 1 Score: 103.219 bits (256), Expect = 8.988e-23 Identity = 93/361 (25.76%), Postives = 166/361 (45.98%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSS-TGIGKALNLTLKEVY-----PTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHIC----------PIPDPGLEKPCT-LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS 1260
D++F++DGS SI +F+ ++F ++T + + RI +++ D F TY KQ +LD + T G+ L L+ + +K V ++ V+ TDG + PY A++L+E ++I IGI D + L +A+ V F L V IC +P+ C D+V+++DSS++I E + + ++FL F+ + ++ K ++ + +SD +F +Y D++ K+ S+ G G AL + SQ R NVP ++ITDGV++ E A +L+M+G + G E N+ +L+ I++
Sbjct: 34 DIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYED-KQEILDFIKVLPYKTGGTNTGEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITDGNSQDEVEPY--AEELREKGIKIYAIGI-KDADEKLLKEIATQPYDQHLFSVSDFEALQGIMLNVTQEICTTVEREGEINSVPE------CNEFAAADIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSSDAVE--EPAQKLRMQGVITFVVKTG-EANILQLRAIAN 381 HSP 2 Score: 102.064 bits (253), Expect = 2.344e-22 Identity = 94/396 (23.74%), Postives = 189/396 (47.73%), Query Frame = 1
Query: 205 SLTAACTFIIR-DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTY-------TKLKQMLLDVTNTR---NLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPY-VYADKLKEANVQILTIGIGSDININELTRLA-SPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ--RPNVPFNILIITDGVNN---MFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
++ C F D+ F+LD S SI+ DDF+ K F + + + + + RI +KF D A IVF TY +K + ++ TR LY A+ K+ T +++V +L+++ TDG + P V A++L++ NV I +G+ + + EL ++ SP + V+++ L +++++ IC + C D+V ++D ++++ D++Q K+ + + + + R+A++ +S + +F ++Y D + ++ ++ + G T G+AL EV + RPNV ++++TD + + P A L+ K N+ ++G+G + L + S + +G L + L + + +IC
Sbjct: 809 TIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLGLY-------AMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGV-KNADTAELEDMSGSPKRTFYVQNYDALKLIKTKILKEICSF------EACNDLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQLKGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDSSSKDDVVAP-----AMALREKNVNVYAVGMGHASQSELLDISGSYETAYLENPFGSL-QVLGSELVFMIC 1184 HSP 3 Score: 97.8265 bits (242), Expect = 5.106e-21 Identity = 98/390 (25.13%), Postives = 176/390 (45.13%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKE-----VEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICP------IP---DPGLEKPCTL-EQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDS--KLISQQRPNVPFNILI-ITDGVNNMFPRPLEY-ANRLKMKGANIISLGIGEEINLKELK-MISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
+ DV+FI+D S ++ ++F+L + F ++ ++ + + R+A + + D+ T++ ++L V G+AL V+ R V+++ V+ TDG +++ AP A L+ + V + +GI S N+ E+ V +FT L V IC IP + ++K C + D+ +LD S SI+ +D++ K F+++FL F++GP++ R+ ++ F+D F Y + VK L GG T L + K Q R +LI ITDG + P++ A L+ + I ++G+ + + EL+ M S + V+ Y D K +KT + IC
Sbjct: 615 VADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKSSDDVSAP---AADLRRSGVTVFAVGIKSFDNLKEMASYPQDKFVFSVGNFTKLNALSGVVTKRICRDISSVFIPLLKNITIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLGLYAMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGV-KNADTAELEDMSGSPKRTFYVQNY-DALKLIKTKILKEIC 999 HSP 4 Score: 90.1225 bits (222), Expect = 1.649e-18 Identity = 90/376 (23.94%), Postives = 168/376 (44.68%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTL---KEVYPTMR-KEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPG-LSLQVESFTGLLKTQSQVVSHICPIPDPGLEKP-CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVI 1335
D++F++DG+ +I+K+ F+ K F + ++T + R + + D A+ VF Y ++L ++ R + +AL TL Y R + V +++ L T+G + + L + V + +G+ + EL R+A + V++++ L + +C L KP C E DLV+++D S S+S D+ K+ L+ + E+ ++ RV++ F D F Y + +++L + +T ALR + + + S+ V N+L+ITDG L L+ K I ++GIG+ IN L I+ + + +E++ L LKT V+
Sbjct: 1195 DIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFTLNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEGPVADSFSLADWPANLAASEVNMFAVGV-AGAREAELRRIAGNNQRAFYVDTYSQLETLCKPITQQLC-----NLTKPVCEKEVADLVILIDGSESVSALDWGTLKSSLINLVRKLEIAQDRWRVSVAQFGDAAPDRFYLNKYNNLAGVEQGIRALSQRRQSTNTWTALRFLQDYFEPEHGSRMNARVSQNLLLITDG-RATDKEDLGVLASLRAKNVEIFAVGIGKNINRHALLKIAGSKERVFLESFESL--PLKTTTAKVL 1561 HSP 5 Score: 82.8037 bits (203), Expect = 3.304e-16 Identity = 95/400 (23.75%), Postives = 187/400 (46.75%), Query Frame = 1
Query: 199 ITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLY----SSTGIGKALNLTLKEVY-PTMRKEV----EKLVVLFTDGT---NNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPG-LEKPCTL-EQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPN-----VPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
I S + A + D+ F++D + S+ + + + F ++ + NRI +F ++ E F F K K + V + RNL IG A+N + P + ++ +++ T G+ + + P+ ++K+ V ++ +G+G +++EL +ASP LS +++S T + Q + + P+ + + C L E D+V I+D S ++ E+++ +NFL +S ++G +K RVA++ +SDE R D + +++ VK L G G+AL+ + +++R + V +++TDG ++ P A L+ G + ++GI NLKE+ + + +V + L +L V+ IC
Sbjct: 412 IDSQSQAFSLKFADIFFLVD--SAASRQESQQIRNFISRLVNQLNVGKNTNRIGLAQFSENVEEEFLFDN-DKTKNEI--VASIRNLQLKPKGLRRIGNAINHARTRFFTPAAGSRIAEGFKQFLLVATAGSSVDDVVQPS-----RQIKKEPVNLIAVGVGRP-DMDELKDIASPTLSYKLDSRT--MPVLPQQIKAVIDSPEGHKVTEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKSSDDVSAP---AADLRRSGVTVFAVGIKSFDNLKEMASYPQDKFVFSVGNFTKL-NALSGVVTKRIC 794 HSP 6 Score: 71.633 bits (174), Expect = 1.213e-12 Identity = 84/397 (21.16%), Postives = 179/397 (45.09%), Query Frame = 1
Query: 217 ACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK--EVYPTMRKEVEKLVVLFTDGTN-NMYPAPYVYADKLKEANVQILTIGIGS-------DININ-ELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQVE 1359
AC ++ DV+F++DG+ ++ DF+ AK + + R+A +++ + ++ F + ++ + + L T G+AL + E R V + +V+ TD ++ + AP A L+E NV + +G+G DI+ + E L +P SLQV S++V IC + ++P E D++ ++D + +I++E ++ K F+ ++ E+G ++ R ++ ++D + F YY +++ + L GG +AL+ + ++ +VP + +IT+G V + F ++ L N+ ++G+ + ++ + + V+TY L+ K + + + +P E
Sbjct: 1003 ACNDLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQLKGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDSSSKDDVVAP---AMALREKNVNVYAVGMGHASQSELLDISGSYETAYLENPFGSLQV--------LGSELVFMIC---NTECKRP---ELIDIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFTLNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEGPVADSFSL-ADWPANLAASEVNMFAVGVAGAREAELRRIAGNNQRAFYVDTYSQLETLCKPITQQLCNLTKPVCE 1381 HSP 7 Score: 58.9214 bits (141), Expect = 1.028e-8 Identity = 54/196 (27.55%), Postives = 90/196 (45.92%), Query Frame = 1
Query: 196 QITSLT-AACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYP----TMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGL-LK-TQSQVVSHIC 762
Q+ +LT C + D++ ++DGS+S+S D+ K +++ ++ + R++ +FGD A F Y L + + ST AL P M V + ++L TDG V A L+ NV+I +GIG +IN + L ++A + +ESF L LK T ++V+ IC
Sbjct: 1371 QLCNLTKPVCEKEVADLVILIDGSESVSALDWGTLKSSLINLVRKLEIAQDRWRVSVAQFGDAAPDRFYLNKYNNLAGVEQGIRALSQRRQSTNTWTALRFLQDYFEPEHGSRMNARVSQNLLLITDGRATDKEDLGVLA-SLRAKNVEIFAVGIGKNINRHALLKIAGSKERVFLESFESLPLKTTTAKVLQAIC 1565
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: si:ch1073-291c23.1 (vitrin [Source:HGNC Symbol;Acc:HGNC:12697]) HSP 1 Score: 100.908 bits (250), Expect = 3.822e-22 Identity = 84/335 (25.07%), Postives = 140/335 (41.79%), Query Frame = 1
Query: 202 TSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM---RKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGI--------GSDININELTRLASPGL------------SLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG 1137
TS T C D+ F++DGS SI K F++ K F ++ +I + I++GDD F + Y+ K++ + + +GKAL+ K + R + V+ DG +P DK++EA+ Q GI G D N E + P SL V S+ L K +V +C K C L D+ ++D S+S+ ++ F+ FE+ RV + ++ E R +F F + D++N +K + + G T G A+ +L S+ +PN +++ITDG
Sbjct: 401 TSHTRDCKV---DIAFLMDGSWSIGKRRFKIQKDFLAEVSQVINVGVAGPMMGIIQYGDDPVTEFSLRQYSNSKELKPAIDKIMQKGGLSNVGKALSYINKHYFSDANGNRGGAPNVAVVLVDG----WPT-----DKVEEASRQARESGINIFFVTIEGPDDN--EKHNVVEPNFVDKAVCRTNGFFSLPVASWFALRKAVQPLVKRVCDTDRLVCSKTC-LNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQHNTKADLLNAIKRINYWSGGTSTGAAITYAAEQLFSKSKPNKRKIMIVITDG 720
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009632.1 (pep scaffold:Pmarinus_7.0:GL479939:9900:31909:-1 gene:ENSPMAG00000008703.1 transcript:ENSPMAT00000009632.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 105.531 bits (262), Expect = 3.320e-24 Identity = 102/396 (25.76%), Postives = 182/396 (45.96%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDA-----EIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHICPI--PDPGLEKP------CTLEQQ-DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE--DKILTVETYGDLKKSLKTVMKTVIC 1338
D+ ++DGS S+ +F L KF ++ + + R+ +++ D + F+ Y +++ + T+ + T G AL TL+ R V K++VL TDG N+ P +D++K + + + ++G+G + EL +ASP + F GL + + S +C + P+ L P C+ +Q DL +++D S+S+ ++ FL K + ++G +K RV M+ +S +R FD G + D K I K + F T G ALR Q RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++S DK + T S+ T + + IC
Sbjct: 2 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGK-ADRKELALMASPPVDKHMFHATDFAGLDSIITALTSTVCTVVQPEKTLAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGF---TFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSIITALTSTIC 386 HSP 2 Score: 101.293 bits (251), Expect = 7.806e-23 Identity = 93/368 (25.27%), Postives = 170/368 (46.20%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDA-----EIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHIC--------PIPDPGLEKPCTL-EQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS 1260
D+ ++DGS S+ + +F L +F ++ + + R+ +++ + + F+ Y K++ + T+ + T G AL TL+ R V K++VL TDG N+ P +D++K + + + ++G+G + EL +ASP + +F GL + + S IC P P + TL + DL +++D S+S+ ++ FL K + +G +K RV M+ +S +R F+ G + D K I + K + F T G ALR Q RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++S
Sbjct: 207 DLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGK-ADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGF---TFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMAS 563 HSP 3 Score: 96.6709 bits (239), Expect = 2.633e-21 Identity = 97/398 (24.37%), Postives = 183/398 (45.98%), Query Frame = 1
Query: 199 ITSLTAACTFIIR-DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDA-----EIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE--DKILTVETYGDLKKSLKTVMKTVIC 1338
+T+ T A T + D+ ++DGS S+ + +F L +F ++ + + R+ +++ + + F Y K++ + T+ + T G AL TL+ R V K++VL TDG N+ P +D++K + + + ++G+G + EL +ASP + + T S +++ P L + + DL +++D S+S+ ++ FL K + ++G +K RV M+ +S +R F+ G + D K I + K + F T G ALR Q RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++S DK + T S+ T + + +C
Sbjct: 398 VTAATQAVTLPSKADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGK-ADRKELALMASPPVDKHMFHATNFAGLDSIIMT--LAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGF---TFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSIITALTSTVC 782 HSP 4 Score: 72.4034 bits (176), Expect = 1.231e-13 Identity = 72/287 (25.09%), Postives = 124/287 (43.21%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVE---------KLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTG---LLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKAL 1062
DV+F +DGS ++ DF L K F ++ + + R+ ++ F+ ++ ++++ V S+ G G+A+ T++ T + E +++V+ T G ++ A Y L+ V+ IGIG+ + +L RL S + L + + G LL QV S I PG D+V LDSS ++ + K FLV+ LS ++G RVA++ F F + + ++ V L GGA +G AL
Sbjct: 804 DVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKV----SAHGGGRAIGATVQFALATAFSQAEGGRAGEGVPQVLVIITGGASS--DAVAHYRGLLRAHGVRSFVIGIGA-FQLLDLQRLPSDPIGLHLYAVGGYAQLLGIAGQVTSVIV---RPGGNGALGPRYADIVFYLDSSATLGAAGFVSLKAFLVQVLSYLQVGRYTVRVAVVQVGGAPVAAFQFNSHSERSALVAAVSQLKAVGGARNVGAAL 1080 HSP 5 Score: 65.4698 bits (158), Expect = 1.715e-11 Identity = 68/323 (21.05%), Postives = 143/323 (44.27%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRK---EVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQ---VESFTGLLKTQ-SQVVSHIC---------PIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQ-------QRPNVPFNILIITDG 1137
D++F LD S ++ F K F +++ ++ R+A ++ G F+F ++++ ++ V+ + + + +G AL+L + + ++ +L T G + A A +L+ + V++ +G+GS ++ + +AS +L V +TGL + +V +I +P L+ DLV ++D S S+ ++ + K F+ + ++N+ RVA+ F R + + +D + + + ++ K G +G +L V S + + QR NV +I II+ G
Sbjct: 997 DIVFYLDSSATLGAAGFVSLKAFLVQVLSYLQVGRYTVRVAVVQVGGAPVAAFQFNSHSERSALVAAVSQLKAVGGARNVGAALSLGYTTYFSQASDAGLQPAQVGILLTGGASADNAA--KAAAQLQASGVRLFVVGVGSGVDQALIQGVASFPYALHSFTVSDYTGLSTVVVTSLVKYIFRPGKMGGTRKLPSDSAAAGRGLDDVDLVFLIDGSTSVEQQTFHLIKKFVERIITNYYYIGGSLRVAVYQFGGAGRLEVPLGNQNDADSLTHTISNIEKVSGPRNLGASLTYVLSNIYKETTTTKLSQRGNVQQSIFIISGG 1317
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009633.1 (pep scaffold:Pmarinus_7.0:GL479939:15464:32536:-1 gene:ENSPMAG00000008703.1 transcript:ENSPMAT00000009633.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 100.908 bits (250), Expect = 9.422e-23 Identity = 96/388 (24.74%), Postives = 179/388 (46.13%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIV-----FEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQ-QDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE--DKILTVETYGDLKKSLKTVMKTVIC 1338
D+ ++DGS S+ + +F L +F ++ + + R+ +++ + F Y K++ + T+ + T G AL TL+ R V K++VL TDG N+ P +D++K + + + ++G+G + EL +ASP + +F GL + + S +C + C+ +Q DL +++D S+S+ ++ FL K + ++G +K RV M+ +S +R F+ G + D K I + K + F T G ALR Q RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++S DK + T S+ T + + +C
Sbjct: 444 DLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGK-ADRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVCTVVQ------CSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGF---TFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSIITALTSTVC 814 HSP 2 Score: 100.523 bits (249), Expect = 1.255e-22 Identity = 99/403 (24.57%), Postives = 180/403 (44.67%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIV-----FEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHIC--------PIPDPGLEKPCTL--------EQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE--DKILTVETYGDLKKSLKTVMKTVIC 1338
D+ ++DGS S+ + +F L +F ++ + + R+ +++ + F+ Y K++ + T+ + T G AL TL+ R V K++VL TDG N+ P +D++K + + + ++G+G + EL +ASP + +F GL + + S IC P P + TL + DL +++D S+S+ ++ FL K + +G +K RV M+ +S +R F+ G + D K I + K + F T G ALR Q RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++S DK + T S+ T + + +C
Sbjct: 232 DLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGK-ADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGF---TFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVC 623 HSP 3 Score: 91.2781 bits (225), Expect = 1.086e-19 Identity = 98/397 (24.69%), Postives = 177/397 (44.58%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIV-----FEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEK-----LVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGL------SLQVESFTGLLKTQSQVVSHICPIPDPGLE-KPCTLEQ-QDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE--DKILTVETYGDLKKSLKTVMKTVIC 1338
D+ ++DGS S+ +F L KF ++ + + R+ +++ D F+ Y +++ + T+ + T G AL TL+ R V K ++VL TDG N+ P +D++K + + + ++G+G + EL +ASP + F L+ + + + + P L C+ +Q DL +++D S+S+ ++ FL K + ++G +K RV M+ +S +R FD G + D K I K + F T G ALR Q RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++S DK + T S+ T + + IC
Sbjct: 26 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKPQVPFILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGK-ADRKELALMASPPVDNLLRFCXDTAIFAVYLEQTVKPIVQLSTLAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGF---TFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSIITALTSTIC 411 HSP 4 Score: 85.1149 bits (209), Expect = 1.231e-17 Identity = 85/343 (24.78%), Postives = 144/343 (41.98%), Query Frame = 1
Query: 199 ITSLTA-ACT-------FIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVE---------KLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTG---LLKTQSQVVSHICPIPDPGLEKPCT---LEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ-------RPNVPFNILIITDG 1137
IT+LT+ CT F DV+F +DGS ++ DF L K F ++ + + R+ ++ F+ ++ ++++ V S+ G G+A+ T++ T + E +++V+ T G ++ A Y L+ V+ IGIG+ + +L RL S + L + + G LL QV S I + C + D+V LDSS ++ + K FLV+ LS ++G RVA++ F F + + ++ V L GGA +G AL + SQ RP P + I+ G
Sbjct: 806 ITALTSTVCTVVQEARPFAKLDVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKV----SAHGGGRAIGATVQFALATAFSQAEGGRAGEGVPQVLVIITGGASS--DAVAHYRGLLRAHGVRSFVIGIGA-FQLLDLQRLPSDPIGLHLYAVGGYAQLLGIAGQVTSVI-------VRSSCVTRGAQYADIVFYLDSSATLGAAGFVSLKAFLVQVLSYLQVGRYTVRVAVVQVGGAPVAAFQFNSHSERSALVAAVSQLKAVGGARNVGAALSLGYTTYFSQASDAGLQVRPGPPAQVGILLTG 1134
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009635.1 (pep scaffold:Pmarinus_7.0:GL479939:22902:31906:-1 gene:ENSPMAG00000008703.1 transcript:ENSPMAT00000009635.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 98.2117 bits (243), Expect = 5.169e-22 Identity = 88/358 (24.58%), Postives = 164/358 (45.81%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIV-----FEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS 1257
D+ ++DGS S+ + +F L +F ++ + + R+ +++ + F Y K++ + T+ + T G AL TL+ R V K++VL TDG N+ P +D++K + + + ++G+G + EL +ASP + +F GL + + S +C + E DL +++D S+S+ ++ FL K + ++G +K RV M+ +S +R F+ G + D K I + K + F T G ALR Q RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++
Sbjct: 297 DLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGK-ADRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVCTVVQ---EAQTPAPTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGF---TFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMA 640 HSP 2 Score: 60.4622 bits (145), Expect = 4.470e-10 Identity = 61/228 (26.75%), Postives = 104/228 (45.61%), Query Frame = 1
Query: 718 TGLLKTQSQVVSHICPIPD---------PGLEKPCTLEQ-QDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE--DKILTVETYGDLKKSLKTVMKTVIC 1338
+G + Q Q S P+P P L C+ +Q DL +++D S+S+ ++ FL K + +G +K RV M+ +S +R F+ G + D K I + K + F T G ALR Q RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++S DK + T S+ T + + +C
Sbjct: 258 SGTHRVQRQADSR--PVPAGGRLQQRGAPQLSSECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGF---TFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVC 476 HSP 3 Score: 58.151 bits (139), Expect = 2.357e-9 Identity = 47/159 (29.56%), Postives = 80/159 (50.31%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIR-------FDFG-FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS 1260
DL +++D S+S+ ++ FL K + ++G +K RV M+ +S ++R FD G + D K I K + F T G ALR SQ RP V ++++TDG N+ P ++ +K G + S+G+G+ + KEL +++S
Sbjct: 1 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGF---TFTGAALRHTLQLFKSQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMAS 152
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007296.1 (pep scaffold:Pmarinus_7.0:GL477669:89164:98084:1 gene:ENSPMAG00000006585.1 transcript:ENSPMAT00000007296.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 92.4337 bits (228), Expect = 5.539e-20 Identity = 82/367 (22.34%), Postives = 166/367 (45.23%), Query Frame = 1
Query: 211 TAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNT-----RNLYSSTGIGKALNLTLKEVYPTMRKE-VEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPN------VPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE 1263
TA + D++F++DGS SI +F+ + F ++ + + + R+ ++ DA F TY K LL+ T N + GI + L + + + + V ++V++ TDG + + P V K++ + IGI D +I+EL ++AS S V+ F+ L Q++ +C + + + + D+V I+D S+++ + +E + + K S ++ P++NR+ ++ ++D +F + +D++ ++ GG G +R + +L ++Q + +L+ + R A LK G + +LG + NL EL +I S+
Sbjct: 27 TACSKASVADIVFLIDGSWSIGNINFQQMRSFLYTLVEGLDVAPDKVRVGMAQYSGDARTEFLMNTYAD-KASLLNYLKTLPYKGGNTKTGLGIEFLMQNHLTDAAGSRKAQNVPQVVIIITDGKSQDDVAGPAV---KMRNMGASVYAIGI-KDADISELGQMASSPASKFVYNVDDFSALAGISEQILQTVCVTVEEIVTEIRKPKFADIVFIMDGSDNVGKAGFEDMRKVVYKIASILDVAPDQNRIGVVQYNDNGHAEFLLNTHSSKEDVLKNIRQFKFRGGIANTGAGIRFAEERLFTEQAGSRKAEGFAQIAVLLTGSASQDEVER---RAKALKDSGVTVFALG-ANKANLNELNLIGSD 384 HSP 2 Score: 64.3142 bits (155), Expect = 4.237e-11 Identity = 76/378 (20.11%), Postives = 161/378 (42.59%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNT--RNLYSSTGIGKAL---NLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQ--------VESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQ-QDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATG-IGKALREVDSKLISQQ-----RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS---EDKILTVETYGDL 1299
D++FI+DGS ++ K FE +K + +I+ + +NRI +++ D+ F T++ + +L ++ R ++TG G L ++ + ++ VL T + A LK++ V + +G + N+NEL + S P + ++S GL++ +V+ G+ E+ D+V +LD ++++ ++E+ + ++ + +G +K R+ M + + F + +++ V+S + GG G +R + +Q R P I ++ G + + A +K G + ++G L EL ++S ++ V +GDL
Sbjct: 232 DIVFIMDGSDNVGKAGFEDMRKVVYKIASILDVAPDQNRIGVVQYNDNGHAEFLLNTHSSKEDVLKNIRQFKFRGGIANTGAGIRFAEERLFTEQAGSRKAEGFAQIAVLLTGSASQ--DEVERRAKALKDSGVTVFALG-ANKANLNELNLIGSDPSANFVFYQRNFRILQSLLGLIQINIEVIE--------GVHFFAPAERYADIVFLLDDTDNVGSANFEELRKLVIMLIEGLNVGKDKVRIGMAQYGRQPSTTFFLNAHGTKEEVAAGVRSAVFRGGPRANTGSGMRFLQENHFVEQAGSRVRQGFP-QIAVLLTGSASQDNASMG-AEAIKGAGGTVFAIG-ANRARLAELNRVASSPPDNHAFYVNNFGDL 595 HSP 3 Score: 47.7506 bits (112), Expect = 5.673e-6 Identity = 54/297 (18.18%), Postives = 118/297 (39.73%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTY-----TKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS----------PGLSLQVESF------------TGLLKTQSQ---VVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGG 1038
DV+F+LD S ++ + +FE K F ++ + ++ R+ ++ D+ F T+ + L T R + + L + + E + + G+++ +A LK+ V++ +G + +L L S P S SF TG+L SQ V+ +P P + T D+V ++D S+++ +E++ + + + ++ + R+ + +S + R +F + + D+ + ++ I+F G
Sbjct: 625 DVVFLLDSSDAVGRKNFEGMKTFVGQIVEYLDVDASKVRVGLAQYSDNPRNEFFLNTHGTKPDVQAAIQLARFTGGRGANTGEAVRSLLQNHFTAQAGSRKDEGYTQIAVILTGSDSQDVVEQ-HAGALKQEGVKVFALG-AEQARLPQLNALGSLPSEQHVLYAPNFSELFSSFDRFLKYLRLKIHTGILAGASQSVIVIYWGVKVPMPCVSP--TEGYADVVFLVDGSDAVGQENFRHSLGLVSRVVDALDVDKDHIRIGLAQYSGDPRAEFFLSTHAAKKDVTDAIRG-IRFKG 916
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007304.1 (pep scaffold:Pmarinus_7.0:GL477669:89164:98057:1 gene:ENSPMAG00000006585.1 transcript:ENSPMAT00000007304.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 92.0485 bits (227), Expect = 6.527e-20 Identity = 82/367 (22.34%), Postives = 166/367 (45.23%), Query Frame = 1
Query: 211 TAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNT-----RNLYSSTGIGKALNLTLKEVYPTMRKE-VEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS---LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPN------VPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE 1263
TA + D++F++DGS SI +F+ + F ++ + + + R+ ++ DA F TY K LL+ T N + GI + L + + + + V ++V++ TDG + + P V K++ + IGI D +I+EL ++AS S V+ F+ L Q++ +C + + + + D+V I+D S+++ + +E + + K S ++ P++NR+ ++ ++D +F + +D++ ++ GG G +R + +L ++Q + +L+ + R A LK G + +LG + NL EL +I S+
Sbjct: 27 TACSKASVADIVFLIDGSWSIGNINFQQMRSFLYTLVEGLDVAPDKVRVGMAQYSGDARTEFLMNTYAD-KASLLNYLKTLPYKGGNTKTGLGIEFLMQNHLTDAAGSRKAQNVPQVVIIITDGKSQDDVAGPAV---KMRNMGASVYAIGI-KDADISELGQMASSPASKFVYNVDDFSALAGISEQILQTVCVTVEEIVTEIRKPKFADIVFIMDGSDNVGKAGFEDMRKVVYKIASILDVAPDQNRIGVVQYNDNGHAEFLLNTHSSKEDVLKNIRQFKFRGGIANTGAGIRFAEERLFTEQAGSRKAEGFAQIAVLLTGSASQDEVER---RAKALKDSGVTVFALG-ANKANLNELNLIGSD 384 HSP 2 Score: 65.855 bits (159), Expect = 1.131e-11 Identity = 68/355 (19.15%), Postives = 150/355 (42.25%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNT--RNLYSSTGIGKAL---NLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS---PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATG-IGKALREVDSKLISQQ-----RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS 1260
D++FI+DGS ++ K FE +K + +I+ + +NRI +++ D+ F T++ + +L ++ R ++TG G L ++ + ++ VL T + A LK++ V + +G + N+NEL + S +F G L + ++++ + + +E D+V +LD ++++ ++E+ + ++ + +G +K R+ M + + F + +++ V+S + GG G +R + +Q R P +++T + A +K G + ++G L EL ++S
Sbjct: 232 DIVFIMDGSDNVGKAGFEDMRKVVYKIASILDVAPDQNRIGVVQYNDNGHAEFLLNTHSSKEDVLKNIRQFKFRGGIANTGAGIRFAEERLFTEQAGSRKAEGFAQIAVLLTGSASQ--DEVERRAKALKDSGVTVFALG-ANKANLNELNLIGSDPSANFVFYQRNFRGFLSSLARILQSLLGLIQINIEVIEGERYADIVFLLDDTDNVGSANFEELRKLVIMLIEGLNVGKDKVRIGMAQYGRQPSTTFFLNAHGTKEEVAAGVRSAVFRGGPRANTGSGMRFLQENHFVEQAGSRVRQGFPQIAVLLTGSASQ--DNASMGAEAIKGAGGTVFAIG-ANRARLAELNRVAS 580 HSP 3 Score: 51.6026 bits (122), Expect = 3.645e-7 Identity = 72/390 (18.46%), Postives = 159/390 (40.77%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTY-----TKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDI--NINELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPC---TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATG-IGKALREV-DSKLI----SQQRPNVP-FNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE---DKILTVETY-------GDLKKSLKTVMK 1326
DV+F+LD S ++ + +FE K F ++ + ++ R+ ++ D+ F T+ + L T R + + L + + E + + G+++ +A LK+ V++ +G +N L L S L +F+ L + + + ++ G+ T D+V ++D S+++ +E++ + + + ++ + R+ + +S + R +F + + D+ + ++ + GG +G +G AL+ + D+ + S+ + P +L+ G + L A LK G + ++G G L EL S+ D + + G L + L ++MK
Sbjct: 630 DVVFLLDSSDAVGRKNFEGMKTFVGQIVEYLDVDASKVRVGLAQYSDNPRNEFFLNTHGTKPDVQAAIQLARFTGGRGANTGEAVRSLLQNHFTAQAGSRKDEGYTQIAVILTGSDSQDVVEQ-HAGALKQEGVKVFALGAEQARLPQLNALGSLPSEQHVLYAPNFSELFSSFDRFLKYLRLKIHTGILAGGVSPTEGYADVVFLVDGSDAVGQENFRHSLGLVSRVVDALDVDKDHIRIGLAQYSGDPRAEFFLSTHAAKKDVTDAIRGIRFKGGRSGNMGAALQHLKDNHFVEAAGSRVKEGFPQIAVLVAGSGSGD---DVLASAQALKNDGTTVFAIGAGAA-KLGELVTAGSDPEKDYVFYQRDFRALSGLSGQLAEGLSSLMK 1014
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO47555 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM80]) HSP 1 Score: 94.3597 bits (233), Expect = 6.216e-21 Identity = 99/404 (24.50%), Postives = 185/404 (45.79%), Query Frame = 1
Query: 238 DVLFILDGSQSI---SKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT-MRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS----------------PGL-SLQVESFTGLLKTQSQVVSH-ICPIPDPGLEKP-----------CTLEQQDLVVILDSSNSI---SEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQ-RPNVPFNILIITDGV-NNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTV 1332
DV F+LDGS S+ +K +F+ K F + S ++ + F D+ + F+F + + V T+ T GK L L ++Y +R V +++++ TDG +++ P A L++ V+I +GIG + + +L + S P + +++ ++ G+ K Q++ SH P+ D L+ + D+ +LD S S+ ++ ++++ KNF+ KF+ +F + + + ++ FS + +F F D+Y + + V + G T GK L L S R VP ++++TDG+ ++ P A L+ G I +LGIG+ + +L + S+ K V T D K L V++T+
Sbjct: 7 DVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATKYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSDDVAGP---AKALRDMGVEIFALGIGKNYDQGQLDSMGSDPKPDHVVTADFDKLDPVIQTIKDKACNGIDKEQTE--SHSASPVDDGALKTKNQNRFRSSSVTVCKAKVDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATNYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSDDVAGP---AKALRDMGVEIFALGIGKNYDQGQLDSMGSDPKPDHVVT-ADFDK-LDPVIQTI 400
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO50079 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFD8]) HSP 1 Score: 82.8037 bits (203), Expect = 1.346e-18 Identity = 50/178 (28.09%), Postives = 83/178 (46.63%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNN---MYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHIC 762
D++F +D S S+ K +FE K F + ++ S T R+A I + + F+F YT ++ V R L T G+AL L ++ ++ K+++L TDG ++ + P + LK VQI +G+G ++ NEL +AS + SFT L + C
Sbjct: 7 DLVFAIDASSSVGKVNFERVKGFIRRLVESFHISRTSTRVAAIVYSSRPRVAFDFNRYTSARRAAHAVKRLRFLRGGTSTGRALRLASSRLFRRYGRKRRKVLMLITDGKSSDDVLKP-----SKALKRKGVQIFAVGVGMSVSRNELILIASHPSQVYQASFTSLSAIVKSLARKTC 179 HSP 2 Score: 76.2554 bits (186), Expect = 2.570e-16 Identity = 47/172 (27.33%), Postives = 88/172 (51.16%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMK 1326
DLV +D+S+S+ + ++E+ K F+ + + +F + RVA + +S R F F Y + + VK L G T G+ALR S+L + +++ITDG ++ L+ + LK KG I ++G+G ++ EL +I+S + ++ L +K++ +
Sbjct: 7 DLVFAIDASSSVGKVNFERVKGFIRRLVESFHISRTSTRVAAIVYSSRPRVAFDFNRYTSARRAAHAVKRLRFLRGGTSTGRALRLASSRLFRRYGRKRRKVLMLITDGKSS--DDVLKPSKALKRKGVQIFAVGVGMSVSRNELILIASHPSQVYQASFTSLSAIVKSLAR 176
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO30053 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1W7]) HSP 1 Score: 78.5666 bits (192), Expect = 4.727e-16 Identity = 49/175 (28.00%), Postives = 93/175 (53.14%), Query Frame = 1
Query: 814 LVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDS-----KLISQQRPNVPFNILIITDGVNNMFP--RPLEYA-NRLKMKGANIISLGIGEEINLKELK-MISSEDKILTVETYGDLKKSL 1311
L+ +LDSS S+ ++Y++ KNF+ +S +G E RV ++ +S E TDY ++D+ + + +G T I K L D + + RP +P ++++TDG P +PL+ A LK K +++G+G I+ +EL+ ++ +D ++ V + DL+ ++
Sbjct: 1 LIFLLDSSGSVGLQNYQKEKNFIKDIVSKQNIGRELTRVGVVVYSHEAIVAIKLTDYSSTEDLNKAIDGIDYYGKTTRIDKGLMTADRIDQVFTVAAGSRPYLPRVLVLLTDGRQTRAPGYKPLKTAVCPLKKKQVKTLAVGVGRGIDARELRELVDHDDDMVMVSDFDDLRTAV 175 HSP 2 Score: 60.077 bits (144), Expect = 5.769e-10 Identity = 32/90 (35.56%), Postives = 47/90 (52.22%), Query Frame = 1
Query: 793 CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKAL 1062
C + D+ ILD+S S+S D++Q K F+ F + PEK RVA+LSFS + + TD+Y + V + G T I +AL
Sbjct: 250 CPVAVVDICFILDASGSVSPIDFKQQKAFVKALAKTFLISPEKTRVAILSFSTIAKLNIKLTDFYDYKALTKAVDKIPYMGFTTRIDRAL 339
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO40329 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7H1]) HSP 1 Score: 77.411 bits (189), Expect = 9.058e-16 Identity = 54/184 (29.35%), Postives = 89/184 (48.37%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLL----DVTNTRNLYSSTGIGKALNLTLKEVY---PTMRKEVEKLVVLFTDGTNNMYPAPY----VYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSH 756
D+ F+LD S SI + +E K F K ++ + S+ + I F D I F Y ++ + D+ R T I KAL L K ++ MR++ +K+ VL +DG P+ + A+ LK VQ+ T+GIG+D+++ EL +AS S+ + + LK + +SH
Sbjct: 109 DIAFLLDASGSIGRRSWEEIKNFVKSIVDMCDISDQGTHVGIITFSTDPVIDIAFDKYKGVEMNAVNIKRDIDELRRKKGYTFIDKALTLADKSLFTQEAGMREDSQKVAVLMSDGIQTKDRGPFTPTDIAANPLKMKGVQVYTVGIGADVDVFELMAVASGITSMFSDRYLDDLKAELAYISH 292 HSP 2 Score: 66.2402 bits (160), Expect = 4.427e-12 Identity = 61/229 (26.64%), Postives = 102/229 (44.54%), Query Frame = 1
Query: 682 ASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDM--IN---KVKSLIKFGGATGIGKALREVDSKLISQQ---RPNVPFNILIITDGVNNMFPRPLE----YANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETY-GDLKKSLKTVMKT 1329
SPG + LK + + CPI D+ +LD+S SI +E+ KNF+ + ++ + V +++FS + D F D YK +M +N + L + G T I KAL D L +Q+ R + ++++DG+ P AN LKMKG + ++GIG ++++ EL ++S + + Y DLK L + T
Sbjct: 80 CSPGERCCPQENECPLKCRKTIKKKSCPI--------------DIAFLLDASGSIGRRSWEEIKNFVKSIVDMCDISDQGTHVGIITFSTDPVIDIAF-DKYKGVEMNAVNIKRDIDELRRKKGYTFIDKALTLADKSLFTQEAGMREDSQKVAVLMSDGIQTKDRGPFTPTDIAANPLKMKGVQVYTVGIGADVDVFELMAVASGITSMFSDRYLDDLKAELAYISHT 293
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO32432 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV23]) HSP 1 Score: 74.3294 bits (181), Expect = 1.148e-15 Identity = 45/157 (28.66%), Postives = 84/157 (53.50%), Query Frame = 1
Query: 220 CTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS 687
CT ++ F++DGS SI+ F ++F K M S T ++A + + ++ E++F F Y + ++ V N +T GKAL TL+EV+ RK V+ +++ TDG +++ P A +++ +++ +G+GS +I EL +A+
Sbjct: 1 CTTKKLNLAFVVDGSGSINNARFGRFREFVKKMAESFPVSATNTQVATVVYSEEPELIFNFGKYNDINEIKTAVDNMPYHGKTTHTGKALKFTLEEVFKKARKNVKNVLIALTDGHASDLVKKP---AQAVRDYGIEVFAVGVGSP-DIAELEEIAT 153 HSP 2 Score: 57.7658 bits (138), Expect = 7.739e-10 Identity = 40/168 (23.81%), Postives = 86/168 (51.19%), Query Frame = 1
Query: 793 CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSE---DKILTVE 1284
CT ++ +L ++D S SI+ + + + F+ K +F + +VA + +S+E F F Y +++ V ++ G T GKAL+ ++ + R NV ++ +TDG +++ +P A ++ G + ++G+G ++ EL+ I+++ D + V+
Sbjct: 1 CTTKKLNLAFVVDGSGSINNARFGRFREFVKKMAESFPVSATNTQVATVVYSEEPELIFNFGKYNDINEIKTAVDNMPYHGKTTHTGKALKFTLEEVFKKARKNVKNVLIALTDGHASDLVKKP---AQAVRDYGIEVFAVGVGSP-DIAELEEIATDPDKDHVFNVD 164
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: COL6A6 (collagen type VI alpha 6 chain [Source:HGNC Symbol;Acc:HGNC:27023]) HSP 1 Score: 120.168 bits (300), Expect = 2.970e-28 Identity = 97/386 (25.13%), Postives = 189/386 (48.96%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK----EVYPTMRKEVEKLVVLFTDG----TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS--LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQP 1350
D++F++DGS SIS ++F+ +KF M+ R + + +D +++F+ + +++L ++ + T GKAL L+ E +V +++++ TDG NN+ ++ L+ V I +IG+ N+ +L +A S V++F L + +C P EK +Q DLV ++D S SI DY+ KNF + + +F +G + RV + FS + +F + + ++ + + + GG T IG AL+ + ++ S++ + N+++ITDG + P A+ L+ G I ++GIG+ +L+ L++ + ++ TV+ +G L+ + V+ T IC ++P
Sbjct: 981 DIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYSNDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDATDRNNLVEP----SEALRSKGVNIFSIGV-KGANMTQLEIMAGQDKSKVFFVDNFAALEGLYKNISKELCNSTKPVCEK----QQADLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILEMTQTGGGTNIGLALKSIREYFETSRGSRRSEGISQNLVLITDGESQDDVEDP---ADDLRALGIEIFAIGIGDVHDLELLQITGTPQRLFTVQNFGSLENIKQKVVDT-ICKSKP 1353 HSP 2 Score: 109.768 bits (273), Expect = 6.034e-25 Identity = 95/378 (25.13%), Postives = 177/378 (46.83%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLT---LKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQ--QRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
D+ F++D S+SI DF+ F + + + R+ +K+ D E+ F +TY + + V + T G+AL + + +V + +V+ TDG +++ +P A+KL+ V I TIG+ + + EL ++ P V F L + +V++ IC + C DLV ++DSS SI +DYE+ K+F+ +S +G + V +L FS + +F ++ +DM+ + + + GG T G AL V S RP++ ++++TDG + P A L+ KG I ++G+ + + L++ S D++ + + D K L++++ +C Q
Sbjct: 606 DIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSSDEVKSP---AEKLRSKGVTIYTIGV-KEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDICSADG----EVCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEFDLRSFFSKEDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDGQAQDEVRGP---AEALREKGVKIYAIGVVDANTTQLLEISGSHDRMYSGRDF-DALKDLESLLALQLCDPQ 971 HSP 3 Score: 106.686 bits (265), Expect = 7.194e-24 Identity = 95/398 (23.87%), Postives = 187/398 (46.98%), Query Frame = 1
Query: 232 IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVY-----PTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQV---ESFTGLLKTQSQVVSHIC----------PIPDPGLEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDS---KLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQV 1356
+ D++F++D S SI +F+L + F +++ + R+ + + D E + T+ + +L + + T G ALN TL+ V+ V+++ V+ TDG + + A L+ + V + ++G+ N EL ++AS V +SF L + ++ +C +++ C E+ D+ ++D S SI D++ NF+++F+ F +GP+ R+ ++ ++D +FG Y ++++ V+++ + GG T G+AL + S K + VP +++ITDG ++ P A +L+ KG I ++G+ +E + KEL+ IS + K + D +K + T IC +V
Sbjct: 397 VADIVFVIDQSGSIGTANFQLVRMFVHSIVSGLDVGQNRVRVGVVVYNDKPEALVYLNTFKNKQNLLNFIQILPYVGGGTKTGAALNFTLQSVFTKESGSRTNTGVQQVAVVITDGESQ--DSVREAAASLRRSGVIVYSVGV-DKANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSSDEVKSP---AEKLRSKGVTIYTIGV-KEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDICSADGEV 787 HSP 4 Score: 77.7962 bits (190), Expect = 1.395e-14 Identity = 53/189 (28.04%), Postives = 105/189 (55.56%), Query Frame = 1
Query: 811 DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLI-----SQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMIS---SEDKILTVETYGDLKKSLKTVMKTVICGNQPQ 1353
D+V ++D S+SI +++E+ + FL + +S ++GP+K RV + +SDE +F D++ + ++ ++ L G T GKA+ + S+ S+ R VP ++ITDG ++ E A +L+ +G + +G+G+ NL EL++I+ ++ + T+E Y L+ ++M+T+ + Q
Sbjct: 23 DIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSS--DDVEEPAQQLREQGVIVFGIGVGQA-NLGELQLIANRPAQRFLFTIENYQALQTLTSSLMQTICVSMEDQ 208 HSP 5 Score: 72.0182 bits (175), Expect = 7.963e-13 Identity = 62/292 (21.23%), Postives = 137/292 (46.92%), Query Frame = 1
Query: 178 TDDQNDQITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVY-----PTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLA---SPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQ--DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVK 1017
T +D +A C + D++F++DGS SI D+FE + F +++++ + + R+ ++ D+ F + + +L + T GKA++ L + + R+ V ++ V+ TDG +++ P A +L+E V + IG+G N+ EL +A + +E++ L S ++ IC + ++ L ++ D+ V++DS IS+ +++Q + L + + LG NR+ + + ++ +F + +++++ +K
Sbjct: 2 TGKGSDSYLFFSAECAMATVADIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVEEP---AQQLREQGVIVFGIGVGQ-ANLGELQLIANRPAQRFLFTIENYQALQTLTSSLMQTICVSME---DQRLALAERFADIFVLVDS--GISQTEFQQVRLLLNRLTNQLNLGASTNRLGLAQYGKVVKVEFLLNKHNTKEEILDSLK 284 HSP 6 Score: 69.707 bits (169), Expect = 4.113e-12 Identity = 84/348 (24.14%), Postives = 157/348 (45.11%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVF---EFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMR-----KEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILD-SSNSISEEDYEQGKNFLVKFLSNFEL------------GPEKNRVA--MLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKA-LREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANII 1206
D++F++D S SI D++L K FT +++ R+ +F + F +F T++ + +L++T T T IG AL +++E + T R + + + +VL TDG + + P AD L+ ++I IGIG ++ L +P V++F L + +VV IC G CT+ D+ + D S+ +S E G N L FL P ++ +A +++ + +DF F + +D++ KV +L +T +A L K +Q + V +LI +DG+++ + + ++ L+ G + +
Sbjct: 1173 DLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILEMTQTGG---GTNIGLALK-SIREYFETSRGSRRSEGISQNLVLITDGESQDDVEDP---ADDLRALGIEIFAIGIGDVHDLELLQITGTPQRLFTVQNFGSLENIKQKVVDTICKSKPSGDPSSCTI---DIAMGFDISTRRVSGESLVSGHNKLQAFLPEIARYLSTVPRLCCTNSPVQSNLAYRVVNVDGRVLYDFNFEAH--DEDVLRKVMAL-SLSESTSFNRAMLGSFREKFEAQSQAGVKV-LLIFSDGLDDDIMKLEQESDLLRQSGVSAL 1506
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: COL6A6 (collagen type VI alpha 6 chain [Source:HGNC Symbol;Acc:HGNC:27023]) HSP 1 Score: 117.857 bits (294), Expect = 1.488e-27 Identity = 95/382 (24.87%), Postives = 186/382 (48.69%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLK----EVYPTMRKEVEKLVVLFTDG----TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLS--LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV----DSKLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
D++F++DGS SIS ++F+ +KF M+ R + + +D +++F+ + +++L ++ + T GKAL L+ E +V +++++ TDG NN+ ++ L+ V I +IG+ N+ +L +A S V++F L + +C P EK +Q DLV ++D S SI DY+ KNF + + +F +G + RV + FS + +F + + ++ + + + GG T IG AL+ + ++ S++ + N+++ITDG + P A+ L+ G I ++GIG+ +L+ L++ + ++ TV+ +G SL+ + + V+C
Sbjct: 795 DIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYSNDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDATDRNNLVEP----SEALRSKGVNIFSIGV-KGANMTQLEIMAGQDKSKVFFVDNFAALEGLYKNISKELCNSTKPVCEK----QQADLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILEMTQTGGGTNIGLALKSIREYFETSRGSRRSEGISQNLVLITDGESQDDVEDP---ADDLRALGIEIFAIGIGDVHDLELLQITGTPQRLFTVQNFG----SLENIKQKVLC 1160 HSP 2 Score: 110.538 bits (275), Expect = 4.129e-25 Identity = 95/378 (25.13%), Postives = 177/378 (46.83%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLT---LKEVYPTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLAS-PGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQ--QRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQ 1347
D+ F++D S+SI DF+ F + + + R+ +K+ D E+ F +TY + + V + T G+AL + + +V + +V+ TDG +++ +P A+KL+ V I TIG+ + + EL ++ P V F L + +V++ IC + C DLV ++DSS SI +DYE+ K+F+ +S +G + V +L FS + +F ++ +DM+ + + + GG T G AL V S RP++ ++++TDG + P A L+ KG I ++G+ + + L++ S D++ + + D K L++++ +C Q
Sbjct: 420 DIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSSDEVKSP---AEKLRSKGVTIYTIGV-KEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDICSADG----EVCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEFDLRSFFSKEDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDGQAQDEVRGP---AEALREKGVKIYAIGVVDANTTQLLEISGSHDRMYSGRDF-DALKDLESLLALQLCDPQ 785 HSP 3 Score: 107.071 bits (266), Expect = 5.740e-24 Identity = 99/414 (23.91%), Postives = 194/414 (46.86%), Query Frame = 1
Query: 190 NDQITSLTAACTFI-IRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVY-----PTMRKEVEKLVVLFTDG-TNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQV---ESFTGLLKTQSQVVSHIC----------PIPDPGLEKPC-TLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDS---KLISQQRPNVPFNILIITDG-VNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQV 1356
+D +A C + D++F++DGS SI D+FE + F +++++ + + R+ ++ D+ F + + +L + T GKA++ L + + R+ V ++ V+ TDG +++ P A +L+E V + I N EL ++AS V +SF L + ++ +C +++ C E+ D+ ++D S SI D++ NF+++F+ F +GP+ R+ ++ ++D +FG Y ++++ V+++ + GG T G+AL + S K + VP +++ITDG ++ P A +L+ KG I ++G+ +E + KEL+ IS + K + D +K + T IC +V
Sbjct: 200 SDSYLFFSAECAMATVADIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVEEP---AQQLREQGVIVFGIA-----NETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSSDEVKSP---AEKLRSKGVTIYTIGV-KEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDICSADGEV 601 HSP 4 Score: 52.7582 bits (125), Expect = 8.593e-7 Identity = 49/172 (28.49%), Postives = 85/172 (49.42%), Query Frame = 1
Query: 793 CTLE-QQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKAL-----REVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETY 1290
CT E + D+V ++D S SI +++Q ++FL +S+F++ P+ R+ + +SD+ R +F + + D++ + L GG T GK L S+ V ++ITDG + A +LK +G + ++GI I L +LK I+ E I TV +
Sbjct: 15 CTQEVKADIVFLVDGSWSIGRPNFQQVRHFLSTLVSSFDVSPDHVRIGLAQYSDDSRTEFLLRTFQNNRDVLQYIARLPYKGGGTMTGKGLDFLLQEHFVEAAGSRASSGVTQIAVVITDGKSQ--DEVKSQAEKLKQRGIILYAIGIKNAI-LTQLKEIADEQNIYTVSDF 183
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: MATN2 (matrilin 2 [Source:NCBI gene;Acc:101164835]) HSP 1 Score: 108.997 bits (271), Expect = 3.005e-25 Identity = 97/421 (23.04%), Postives = 182/421 (43.23%), Query Frame = 1
Query: 220 CTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPT------MRKEVEKLVVLFTDGTNNMYPAPYV--YADKLKEANVQILTIGIGSDININELTRLASPGLS---------LQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQ---------------------------DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREV-DSKLISQQ--RPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKIL------TVETYGDLKKSLKTVM 1323
C I D +F++D S+SI +D+E K F +++ + + R+ +++G + F TY ++ V N +L + T G A+ T++ + + + ++ ++ TDG P V A + ++A +QI IG+G +++ L + S S Q+ES + QS++ +C + G E C DLV ++D S S+ ++E K F+ + + ++ V ++ +S ++R +F Y D + V + G + G ALR + +S S++ RPN ++ TDG + E+A++ K G I +LGIG+ I +EL+ I+SE + + E G++ + LK+ M
Sbjct: 42 CKAIPLDFVFVIDSSRSIRSNDYEKVKTFIINLLQFLDIGHDATRVGLLQYGSVVQPEFSLNTYGSRAEVEQAVKNMEHLATGTMTGLAIQYTMETAFTEDHGARPLNMHIPRIAMIVTDGR----PQDTVKEVAAEARKAGIQIFAIGVGR-VDMKILKTIGSEPHSEHVHLVANFSQIESLISVF--QSKLCVDVCQTLEHGCEHQCVANGSSYMCKCFEGFILAEDKRSCKKCHDGVMDLVFVIDGSKSLGPANFELVKQFVNSIVDSLDISRTGTHVGLIQYSTKVRTEFTLGQYVTGDSIKQAVGRMQYMGRGSRTGSALRHMFESSFSSREGGRPNTQQVTIVFTDGRSQ--DDVSEWASKAKSSGITIYALGIGKAIE-QELREIASEPNEMYLYYAESFEKMGEITQKLKSRM 452 HSP 2 Score: 72.0182 bits (175), Expect = 4.001e-13 Identity = 52/197 (26.40%), Postives = 89/197 (45.18%), Query Frame = 1
Query: 784 EKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNI------LIITDGVNNMFPRPL----EYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKK--SLKTVMKTVIC 1338
E PC D V ++DSS SI DYE+ K F++ L ++G + RV +L + ++ +F Y ++ VK++ T G A++ ++ P N+ +I+TDG RP E A + G I ++G+G +++K LK I SE V + + SL +V ++ +C
Sbjct: 39 ENPCKAIPLDFVFVIDSSRSIRSNDYEKVKTFIINLLQFLDIGHDATRVGLLQYGSVVQPEFSLNTYGSRAEVEQAVKNMEHLATGTMTGLAIQYTMETAFTEDHGARPLNMHIPRIAMIVTDG------RPQDTVKEVAAEARKAGIQIFAIGVG-RVDMKILKTIGSEPHSEHVHLVANFSQIESLISVFQSKLC 228
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: matn1 (matrilin 1, cartilage matrix protein [Source:NCBI gene;Acc:101155615]) HSP 1 Score: 94.3597 bits (233), Expect = 2.310e-20 Identity = 92/403 (22.83%), Postives = 172/403 (42.68%), Query Frame = 1
Query: 196 QITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKAL----NLTLKEVYPTMRK--EVEKLVVLFTDGTNNMYPAPYV--YADKLKEANVQILTIGIGSDININELTRLASPGLSLQV---ESFTGLLK-----TQSQVVSHICPIPD----------PGLEK-----------------PCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEY----ANRLKMKGANIISLGIGEEINLKELKMISSE 1263
+++S C D++FI+D S+S+ +FE K F ++ + R+ + + + +T+ ++ VT L + T G A+ N+ E+ K ++ ++ ++ TDG P V A + +EA ++I IG+G +++N L ++AS L V ES++ + K ++ VS +C D PG K C+ D+V ++D S S+ E++E K ++ + + ++ K V ++ +S +R +F Y D+ + VK + T G+AL + + RP V ++ TDG R +Y A + K G + ++G+G + ELK I+SE
Sbjct: 2 KLSSSAGLCKTRPTDLVFIIDSSRSVRPSEFEQVKVFLAKVIEGLDVGPNATRVGVVNYASRVKNEVSLKTHRSKPALVKAVTKIEPLSTGTMTGLAIQFAQNVAFSEIEGARTKSPDISRVAIIVTDGR----PQDNVRDVAQRAREAGIEIFAIGVGR-VDMNTLKQMASDPLDDHVDYVESYSVIEKLTKKFQEALCVSDLCATGDHDCEQVCISTPGSYKCACKDGFTLRDDGRTCSACSNAATDVVFVIDGSKSVRPENFELVKKWINQIIDKLDVSDAKTHVGLVQYSSSVREEFPLGRYNNKKDLKDAVKKMAYMERGTMTGQALNFMVENSFGRSRPGVAKVGIVFTDG------RSQDYIGDAAKKAKDNGYKMYAVGVGNAVE-NELKEIASE 392 HSP 2 Score: 72.7886 bits (177), Expect = 2.262e-13 Identity = 51/198 (25.76%), Postives = 94/198 (47.47%), Query Frame = 1
Query: 211 TAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLE 804
+AC+ DV+F++DGS+S+ ++FEL KK+ ++ + S+ + + +++ F Y K + V + T G+ALN ++ + R V K+ ++FTDG + Y A K K+ ++ +G+G+ + NEL +AS + +T KT +Q+ + I EKPC +
Sbjct: 236 CSACSNAATDVVFVIDGSKSVRPENFELVKKWINQIIDKLDVSDAKTHVGLVQYSSSVREEFPLGRYNNKKDLKDAVKKMAYMERGTMTGQALNFMVENSFGRSRPGVAKVGIVFTDGRSQDYIGD--AAKKAKDNGYKMYAVGVGNAVE-NELKEIASEPTADHY-FYTADFKTMNQIAKKL-QINFCDEEKPCECD 428
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: si:ch1073-291c23.1 (vitrin [Source:NCBI gene;Acc:101168147]) HSP 1 Score: 93.9745 bits (232), Expect = 6.161e-20 Identity = 75/316 (23.73%), Postives = 139/316 (43.99%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTM---RKEVEKLVVLFTDGTNNMYPAPYVY-ADKL-KEANVQILTIGI-GSDIN----------INELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDG 1137
D+ F++DGS SI K F++ K F ++ +I + I++GD+ +TY+ +++ V + +G+AL+ K+ + R + V+ DG +P V A +L +E+ + I + I G D + +++ + SL V S+ L K +V +C K C L D+ ++D S+S+ ++ F+ FE+ RV + ++ E R +F F Y +++N +K + + G T G A+ KL S+ +PN +++ITDG
Sbjct: 302 DLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDG----WPTDKVEEASRLARESGINIFFVTIEGPDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFALRKAVQPLVKRVCDTDRLVCSKTC-LNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDG 612
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000024982.1 (SMESG000024982.1) HSP 1 Score: 837.795 bits (2163), Expect = 0.000e+0 Identity = 414/415 (99.76%), Postives = 415/415 (100.00%), Query Frame = 1
Query: 118 MNLFLAVLPILIGFFTFNVKTDDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQVEY 1362
MNLFLAVLPILIGFFTFNVKTDDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLK+MLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQVEY
Sbjct: 1 MNLFLAVLPILIGFFTFNVKTDDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKEMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQVEY 415
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000046770.1 (SMESG000046770.1) HSP 1 Score: 373.244 bits (957), Expect = 5.927e-127 Identity = 184/344 (53.49%), Postives = 240/344 (69.77%), Query Frame = 1
Query: 316 MMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDE--IRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICG 1341
MMT IK +N +NRI+FI FGDDA ++F F + L M+ V N RN +T IGKAL LT + + P MRK+ ++ V+LFTDGTNN++PAPY+YAD LK+A+V+ILT+GIGS+IN EL LASP +++ V F L + +++ H+C I DP +PC +EQQDLVV++DSS SIS D+E GK F+ +S F++GP+ RV ++SFSD I FD D K K+K L K G ATG+G ALREV K +R +PFNILI+TDG NN+FPRP E A++LK +GANIISLGIG INLKEL ISS DKILTV +Y L+K++KTV+K V CG
Sbjct: 1 MMTFIKTTNADNRISFIVFGDDATLIFNFDKFKNLNDMVRQVKNVRNDNGATAIGKALKLTYENLVPQMRKDAQRYVILFTDGTNNVFPAPYIYADHLKDASVRILTVGIGSEINKKELINLASPNMAITVADFQKLFENLMKIIHHVCVIVDPIPNEPCKVEQQDLVVLMDSSKSISGPDFEIGKRFVAHLISKFKIGPKATRVGLVSFSDHPRIEFDLHLVDRNK------KIKHLRKLGSATGLGLALREVQFKFWPHRRHGIPFNILILTDGYNNVFPRPHEIASKLKREGANIISLGIGRHINLKELTDISSNDKILTVNSYERLQKNMKTVLKAVTCG 338 HSP 2 Score: 100.908 bits (250), Expect = 2.769e-23 Identity = 56/180 (31.11%), Postives = 94/180 (52.22%), Query Frame = 1
Query: 220 CTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHI 759
C +D++ ++D S+SIS DFE+ K+F +++ K R+ + F D I F+ + K+ + + R L S+TG+G AL + +P R + +++ TDG NN++P P+ A KLK I+++GIG IN+ ELT ++S L V S+ L K V+ +
Sbjct: 160 CKVEQQDLVVLMDSSKSISGPDFEIGKRFVAHLISKFKIGPKATRVGLVSFSDHPRIEFDLHLVDRNKK----IKHLRKLGSATGLGLALREVQFKFWPHRRHGIPFNILILTDGYNNVFPRPHEIASKLKREGANIISLGIGRHINLKELTDISSNDKILTVNSYERLQKNMKTVLKAV 335
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000046768.1 (SMESG000046768.1) HSP 1 Score: 132.109 bits (331), Expect = 2.112e-32 Identity = 91/357 (25.49%), Postives = 184/357 (51.54%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEF-QTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKK 1305
D++F++D S + + ++ + + + S T +RI+ I + D+ F + +TK + + + + R+ + + ALN + R +V K+VVLFTD A+ LK V+I+ +G+ + N LAS S+++E+ + K + Q+ + IC + CT+++ D++V +D S S+ E+++ +F+ + + N +L ++ R+ + +F+ E+ + +++ DM ++ + G T IG A+ L ++ R +VP ++++TDG N++ P A + K+ G IIS+G+G++I+ KEL I+S + V+T+ DL+K
Sbjct: 691 DIVFVVDKSTDNTNEIWDGIRSLMLTTLQSLNISRTTSRISIIDYSDETTTEFRLDEAFTK-RDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLASKKSSIKIENINKIDKNKEQIKNMICD-AFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEK 1045 HSP 2 Score: 124.79 bits (312), Expect = 5.986e-30 Identity = 87/305 (28.52%), Postives = 162/305 (53.11%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDM--MTIIKNSNTENRIAFIKFGDDAEIVFEF-QTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVN 1143
D++F++ S + + + F+ F K + +T++ NT R+A +KF I F + Y + + D+T + N IGKAL RK K V++ DG + A+ LK+ +++ TIG+ + ++L +ASP +L V++F+ L + + + S +C + G K C+ +Q DL+ LDSS+SI + ++ +F+ + + + ++ +V +++F+ + R +FG + + MI+ VK +I GAT IG+ALRE ++L++ R VP I+++TDGV+
Sbjct: 1070 DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENT--RVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN---GVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVI-KGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVS 1368 HSP 3 Score: 122.094 bits (305), Expect = 4.846e-29 Identity = 94/364 (25.82%), Postives = 174/364 (47.80%), Query Frame = 1
Query: 220 CTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSH-ICPIPDPGLEKPCTLEQQ-DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKK 1305
CT DV+ +D S S+ ++F++ F ++ + RI F + + + + M ++ + +T IG A+ ++ R++V K++V+ TDG N++ +P + A K K ++I+++G+G I+ EL +AS G V++FT L K ++ +S+ C IP +KP + DL+ ++ SS + + E ++Q +F+ + E RVA+L F+ D GF + YK + + ++ + IGKAL S R N ++II DG E AN LK G + ++G+ + + +LK ++S DK L V+ + +L++
Sbjct: 875 CTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNDACQIP----QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--NFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQR 1232 HSP 4 Score: 118.242 bits (295), Expect = 8.235e-28 Identity = 98/377 (25.99%), Postives = 198/377 (52.52%), Query Frame = 1
Query: 238 DVLFILDGSQSIS-KDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYP-APYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGL-LKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQVE 1359
D++F+LD S S++ D ELA FT +++ ++ +++ + + D E+ + ++ V+ L +T I AL + +E R+ +K V+L TDG +N P V A+KLK+ ++++ IG+G + ++L +++S +E + + L + + C IP P ++ C+ + D+ +I+D S+ + ++ F +F+ FELG K R +++SF++E + FT Y DD++ KV+SL GG T I KAL + S+ + N ++++T+G++ + ++ A L+ G +I+++G+G++ + +ELK++S+ + + + K L+ +K V C P ++
Sbjct: 315 DLIFVLDASSSLALYWDQELA--FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDLAQDACQIPKP--KRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY--DDILKKVESLKHVGGFTRIDKAL-NIASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIVTIGLGDKADREELKILSTFPNPFLRQDFKEEKSLLEKSVK-VSCKTTPAIK 682 HSP 5 Score: 75.8702 bits (185), Expect = 3.577e-14 Identity = 51/190 (26.84%), Postives = 101/190 (53.16%), Query Frame = 1
Query: 778 GLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGI-GKALREVDSKLISQQRPNVPFN--ILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
L++ C + +D+ VILDSS+SI+ +++ + +FLS F + P RV+ + + D ++ +F FT + S + V+ K GG + + G+AL ++ + RP + +LI++DG N + A +++ KG I ++ IG +N+ L ISS+ ++ + ++ + ++ +VIC
Sbjct: 21 ALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGSNLNIPLLLSISSKKNVVMFPEFQTMRGDSENLV-SVIC 209 HSP 6 Score: 72.7886 bits (177), Expect = 3.190e-13 Identity = 62/221 (28.05%), Postives = 110/221 (49.77%), Query Frame = 1
Query: 199 ITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMT--IIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYS-STGIGKALNLTLKEVYPTMR--KEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSI 846
I +L C RD+ ILD S SI+ +F+ + ++ +I +NT R++F+ +GD + F F +T + V R + S+ +G+ALN TL ++ P MR E+++++++ +DG+N + A K++E V I T+ IGS++NI L ++S + F + +VS IC + KP + E + I SS ++
Sbjct: 19 ILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNT--RVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGSNLNIPLLLSISSKKNVVMFPEFQTMRGDSENLVSVICVVK---YTKPPSRETISIPDITSSSATV 234 HSP 7 Score: 56.6102 bits (135), Expect = 3.573e-8 Identity = 47/188 (25.00%), Postives = 91/188 (48.40%), Query Frame = 1
Query: 727 LKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFP--RPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVE 1284
++T+ S+ I P ++ C +Q DL+ +LD+S+S++ ++Q F + + ++ + ++V +L +SD + ++ V L GAT I ALR + IS+ R ++++TDG +N P +P A +LK G +I +G+G + +L +SS + +E
Sbjct: 289 VETKRTTTSNYIEISTP--DQNCNNQQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNS-PTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIE 472
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000043380.1 (SMESG000043380.1) HSP 1 Score: 114.005 bits (284), Expect = 9.656e-27 Identity = 97/380 (25.53%), Postives = 183/380 (48.16%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFG-------FTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICGNQPQV 1356
++ +LD S SI D F+LAK+F D++ +R I F DDAE++F T+ +++ + + + L +T +GKA+ L + R + K+++L TDG NN +P KEA V I+ IGIGS+I+ ELT +ASPGL Q++++ L+ + + C + C E D+ +++S + +Q + + L+ E+ K RVA++ ++ I +T Y K D F + I L E +L+++ R ++P ++++ + F ++ A L+ +GAN++++ IG +++ +L +S+ K + + + + + S + IC N +V
Sbjct: 37 EMAIVLDASSSIEPDHFKLAKQFIVDVIGDFVVGPDHSRFGMISFSDDAELLFTLTTHKTIEETKKAILDAKFLTGATAMGKAIQLAGTGILQESRTGIPKVILLITDGKNNKFPNAAQMGKLQKEAGVTIIGIGIGSEISPQELTEIASPGLYYQLDTYPSLIGLVKTLKTSFCKKLENIQIDTCETE-GDISFVIESQQKAGDLHKKQIE-LITHVLNRLEISEGKQRVAIVVENEGILVPLNKFSSRSEYTQYLKKGD----------FKVGSKISSGLVE-SGRLLAENRTDIPKLVVLMRTQKSENFENDVKAALALRNQGANVLTVAIGLNVDVSQLNELSTTRKTVIFKDF-NFQVSFVNEIAENICQNLKKV 402 HSP 2 Score: 95.9005 bits (237), Expect = 8.231e-21 Identity = 69/283 (24.38%), Postives = 135/283 (47.70%), Query Frame = 1
Query: 523 RKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEK---PCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC---GNQPQ 1353
R ++ KLVVL + + A L+ +LT+ IG ++++++L L++ ++ + F + +++ +IC L+K C ++ D+V +LD S+SI E++ F+ + + ++G + RV + F+ E F Y D++I +++L G T IG AL ++ + R N ++++TDG +NM +P A ++ G +II +GI EI+ +L IS+ ++ + + L M C N+P+
Sbjct: 317 RTDIPKLVVLMRTQKSENFENDVKAALALRNQGANVLTVAIGLNVDVSQLNELSTTRKTVIFKDFNFQVSFVNEIAENICQ----NLKKVYVECIAKKLDVVFVLDCSSSIGPENWMNQLQFVSRIVQMMDVGKDSTRVGSVYFNSEAYVGFSLDKYGTKDEVIKAIRNLPFSEGGTAIGDALNLAHDQMKASLRNNTVPVMILVTDGQSNMGRKPESEALEIRNDGVHIICIGITNEISKDQLIRISTSNRYYHINDFDSLNSVSVEAMTKESCQAVNNKPK 595 HSP 3 Score: 92.0485 bits (227), Expect = 1.486e-19 Identity = 59/182 (32.42%), Postives = 100/182 (54.95%), Query Frame = 1
Query: 793 CTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVIC 1338
C+ + ++ ++LD+S+SI + ++ K F+V + +F +GP+ +R M+SFSD+ F T + ++ + GAT +GKA++ + ++ + R +P IL+ITDG NN FP + K G II +GIG EI+ +EL I+S ++TY L +KT +KT C
Sbjct: 31 CSKNRIEMAIVLDASSSIEPDHFKLAKQFIVDVIGDFVVGPDHSRFGMISFSDDAELLFTLTTHKTIEETKKAILDAKFLTGATAMGKAIQLAGTGILQESRTGIPKVILLITDGKNNKFPNAAQMGKLQKEAGVTIIGIGIGSEISPQELTEIASPGLYYQLDTYPSLIGLVKT-LKTSFC 211 HSP 4 Score: 69.3218 bits (168), Expect = 2.792e-12 Identity = 41/163 (25.15%), Postives = 80/163 (49.08%), Query Frame = 1
Query: 238 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGL 726
DV+F+LD S SI +++ +F ++ ++ R+ + F +A + F Y +++ + N T IG ALNL ++ ++R +++L TDG +NM P A +++ V I+ IGI ++I+ ++L R+++ + F L
Sbjct: 412 DVVFVLDCSSSIGPENWMNQLQFVSRIVQMMDVGKDSTRVGSVYFNSEAYVGFSLDKYGTKDEVIKAIRNLPFSEGGTAIGDALNLAHDQMKASLRNNTVPVMILVTDGQSNMGRKPESEALEIRNDGVHIICIGITNEISKDQLIRISTSNRYYHINDFDSL 574
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000033673.1 (SMESG000033673.1) HSP 1 Score: 83.9593 bits (206), Expect = 9.729e-17 Identity = 54/212 (25.47%), Postives = 97/212 (45.75%), Query Frame = 1
Query: 196 QITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKQMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVS------HICPIPDPGLEK-PCTLEQQ 810
++T C +V+FILDGS S+ D+F K F ++ + N R+A I + ++VF Y K + ++ T +G ALN+ L ++ R+ K+++L +DG N P + A +LK + ++TIG+ + + N + ++ ++ F L K S +V HI P ++K PC ++Q
Sbjct: 33 ELTQEDKKCIKTAGEVIFILDGSTSVGPDNFNKEKDFATQIVNRLVIGNNLTRVALITYNSVPQLVFNVDKYNNSKDVNREIMKAEFTEGITVVGDALNMVLTDIESKTRRSTGKVIILISDGKRNGGMDPILVAKQLKALSYMLITIGLDKETDENSMIAISDKKNFYLIKDFDSLPKIVSTIVQKTCTGFHISKCSAPIVKKGPCVNKEQ 244 HSP 2 Score: 68.9366 bits (167), Expect = 5.855e-12 Identity = 43/186 (23.12%), Postives = 91/186 (48.92%), Query Frame = 1
Query: 784 EKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKKSLKTVMKTVICG 1341
+K C +++ ILD S S+ +++ + K+F + ++ +G RVA+++++ + F Y S D+ ++ G T +G AL V + + S+ R + I++I+DG N P+ A +LK +I++G+ +E + + IS + ++ + L K + T+++ G
Sbjct: 38 DKKCIKTAGEVIFILDGSTSVGPDNFNKEKDFATQIVNRLVIGNNLTRVALITYNSVPQLVFNVDKYNNSKDVNREIMKAEFTEGITVVGDALNMVLTDIESKTRRSTGKVIILISDGKRNGGMDPILVAKQLKALSYMLITIGLDKETDENSMIAISDKKNFYLIKDFDSLPKIVSTIVQKTCTG 223 The following BLAST results are available for this feature:
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009269 ID=SMED30009269|Name=Collagen alpha-6(VI) chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1462bpback to top protein sequence of SMED30009269-orf-1 >SMED30009269-orf-1 ID=SMED30009269-orf-1|Name=SMED30009269-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=416bp MNLFLAVLPILIGFFTFNVKTDDQNDQITSLTAACTFIIRDVLFILDGSQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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