DEK_C domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009265 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of DEK_C domain-containing protein vs. Ensembl Zebrafish
Match: dek (DEK proto-oncogene [Source:ZFIN;Acc:ZDB-GENE-060512-191]) HSP 1 Score: 49.6766 bits (117), Expect = 4.347e-6 Identity = 35/118 (29.66%), Postives = 59/118 (50.00%), Query Frame = 2 Query: 215 GNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFDENDESE-FKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALG--LVEYLRCPFETGSEIFR 559 G G K GDI RI +I KL L K +H + Y G A +++ FNGF ++S+ + K++ V ++ LK IC L++ SG + L ++++L P +G + + Sbjct: 68 GRGDKLGDIARINHSI--GKLKAPLLKPLHKILYDRPGAASTLRKNLRLFNGFPFKEDSDLYNKKMEKVKRLPKKLLKTICQTLDLERSGTQ---IVLSERIMKFLAQPTNSGKPVLK 180
BLAST of DEK_C domain-containing protein vs. TrEMBL
Match: A0A1W4XLF1 (protein DEK isoform X2 OS=Agrilus planipennis OX=224129 GN=LOC108742789 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 7.313e-11 Identity = 51/140 (36.43%), Postives = 83/140 (59.29%), Query Frame = 2 Query: 128 RIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFD-ENDESEFKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALGLVEYLRCPFETG 544 R RK V+R ++ ++ + E+ I + G+GI G+I RI +I R K N +L K +H L Y+ GK K ++IK+FNGFD E D E+KK+L+ + K + LK IC++L++ G K +V+ ++ +L P + G Sbjct: 117 RERKKVNRFNEEFNDSKELSKLEIPE---GSGIPLGEIPRIDASISRFK-NDDL-KVLHRLLYKCQGKTTKTKANIKRFNGFDFEKDSEEYKKKLTWIQKFDLKQLKSICEMLDLEKKG-TKDEVSERIIIFLMEPQDIG 250
BLAST of DEK_C domain-containing protein vs. TrEMBL
Match: A0A1W4XBW7 (protein DEK isoform X1 OS=Agrilus planipennis OX=224129 GN=LOC108742789 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 8.685e-11 Identity = 51/140 (36.43%), Postives = 83/140 (59.29%), Query Frame = 2 Query: 128 RIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFD-ENDESEFKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALGLVEYLRCPFETG 544 R RK V+R ++ ++ + E+ I + G+GI G+I RI +I R K N +L K +H L Y+ GK K ++IK+FNGFD E D E+KK+L+ + K + LK IC++L++ G K +V+ ++ +L P + G Sbjct: 117 RERKKVNRFNEEFNDSKELSKLEIPE---GSGIPLGEIPRIDASISRFK-NDDL-KVLHRLLYKCQGKTTKTKANIKRFNGFDFEKDSEEYKKKLTWIQKFDLKQLKSICEMLDLEKKG-TKDEVSERIIIFLMEPQDIG 250
BLAST of DEK_C domain-containing protein vs. TrEMBL
Match: A0A0T6BH16 (SAP domain-containing protein (Fragment) OS=Oryctes borbonicus OX=1629725 GN=AMK59_1579 PE=4 SV=1) HSP 1 Score: 73.9442 bits (180), Expect = 1.257e-10 Identity = 57/160 (35.62%), Postives = 89/160 (55.62%), Query Frame = 2 Query: 128 RIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFD-ENDESEFKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALGLVEYLRCPFETGSEIF-----RTSALRRKIKG 589 R RK V R +D + + K ++ TG G+ GDI RI +I R K N +L K +H L Y+T GK +IKKFNGFD D E+ K+LS V K ++ LK +C++L++ G K+++ +VE+L P ++G + RT+A++ +G Sbjct: 129 RERKKVQRFNDNLPR--DSKEVTKLEVPTGRGLALGDIPRIDASISRFK-NDDL-KLLHRLLYKTPGKTTMIKKNIKKFNGFDFAKDSDEYGKKLSSVQKFEVKQLKSVCEMLDLEKKGN-KEEIGERIVEFLIEPKDSGKPVGGGRPKRTAAVKANNRG 283
BLAST of DEK_C domain-containing protein vs. TrEMBL
Match: V5I938 (Protein DEK (Fragment) OS=Anoplophora glabripennis OX=217634 GN=DEK PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 6.162e-10 Identity = 54/157 (34.39%), Postives = 91/157 (57.96%), Query Frame = 2 Query: 125 KRIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFD-ENDESEFKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALGLVEYLRCPFETG-SEIFRTSALRRKIKG 589 KR RK+V RL ++ + E K ++ ++G+G GDI R+ +I R K N +L K +H L Y+ +GK +I+KFNGFD D E+ K++S K ++ LK IC++L++ SG K D+ ++++L P ++G RT+A++ +G Sbjct: 144 KRERKNVQRL-EIASEVKETK----VEFVSGSGSSLGDIPRVDASISRFK-NDDL-KLLHRLLYKVVGKNTLIKKNIRKFNGFDFSKDSEEYTKKVSFAHKFEIKQLKSICEMLDLPKSG-TKDDIVDRIIDFLLEPKDSGRGRPKRTAAVKANNRG 292
BLAST of DEK_C domain-containing protein vs. TrEMBL
Match: A0A482W7A9 (DEK (Fragment) OS=Asbolus verrucosus OX=1661398 GN=BDFB_007726 PE=4 SV=1) HSP 1 Score: 69.3218 bits (168), Expect = 4.747e-9 Identity = 53/161 (32.92%), Postives = 91/161 (56.52%), Query Frame = 2 Query: 125 KRIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFD-ENDESEFKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALGLVEYLRCPFETGSEIF-----RTSALRRKIKG 589 KR RK+V R ++ + + + K I G+G G+I RI +I R K N ++ K H + ++T GK+ +IKKFNGFD + D E+ K+++ + K ++ LK IC++L++ +G K D+ ++E+L P ++G I RT+A+R +G Sbjct: 134 KRERKNVQRFNEEF--SSDSKESSKIDIPNGSGTALGNIPRIDASISRFK-NDDM-KLFHRILFKTTGKSTMIKKNIKKFNGFDFKKDSDEYTKKVASMHKCEVKQLKSICEMLDLQKTG-SKDDIIERIIEFLLDPKDSGKAIGGGRPKRTAAVRANNRG 289
BLAST of DEK_C domain-containing protein vs. Ensembl Sea Lamprey
Match: DEK (DEK proto-oncogene [Source:HGNC Symbol;Acc:HGNC:2768]) HSP 1 Score: 45.8246 bits (107), Expect = 7.350e-6 Identity = 21/50 (42.00%), Postives = 31/50 (62.00%), Query Frame = 1 Query: 919 LKEKITAILSGADLSKITLNQVCDGVVDQFPDVDLVLRRPHIKSLVKQVI 1068 L++ + +L GADL +T+ VC V +FPD DL R+ IK VK++I Sbjct: 185 LRQAVRLLLDGADLESVTMKHVCKEVYAKFPDFDLTERKDLIKQAVKELI 234
BLAST of DEK_C domain-containing protein vs. Ensembl Sea Lamprey
Match: DEK (DEK proto-oncogene [Source:HGNC Symbol;Acc:HGNC:2768]) HSP 1 Score: 45.4394 bits (106), Expect = 8.158e-6 Identity = 21/50 (42.00%), Postives = 31/50 (62.00%), Query Frame = 1 Query: 919 LKEKITAILSGADLSKITLNQVCDGVVDQFPDVDLVLRRPHIKSLVKQVI 1068 L++ + +L GADL +T+ VC V +FPD DL R+ IK VK++I Sbjct: 188 LRQAVRLLLDGADLESVTMKHVCKEVYAKFPDFDLTERKDLIKQAVKELI 237
BLAST of DEK_C domain-containing protein vs. Planmine SMEST
Match: SMESG000049193.1 (SMESG000049193.1) HSP 1 Score: 400.208 bits (1027), Expect = 7.202e-156 Identity = 218/219 (99.54%), Postives = 218/219 (99.54%), Query Frame = 2 Query: 68 MENENVIESVNSDINNSREKRIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFDENDESEFKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALGLVEYLRCPFETGSEIFRTSALRRKIKGGKNKPTVKSVKKTKALXXXXXXXXXXXXXXXXIEDGIKSGDEISE 724 MENENVIESVNSDINNSREKRIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFDENDESEFKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALGLVEYLRCPFETGSEIFRTSALRRKIKGGKNKPTVKSVKKTKALSSNDDDDVSMDSSVSNIEDGIKS DEISE Sbjct: 1 MENENVIESVNSDINNSREKRIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFDENDESEFKKRLSIVDKMKMDDLKLICDLLNVHPSGKMKKDVALGLVEYLRCPFETGSEIFRTSALRRKIKGGKNKPTVKSVKKTKALSSNDDDDVSMDSSVSNIEDGIKSSDEISE 219 HSP 2 Score: 170.629 bits (431), Expect = 7.202e-156 Identity = 117/119 (98.32%), Postives = 118/119 (99.16%), Query Frame = 1 Query: 724 NDQIQRETPEKSNGKSIKKEKASNEKQNYVPXXXXXXXXXXXXVPNKXXXXXXXXXXXXXXXXXLLKEKITAILSGADLSKITLNQVCDGVVDQFPDVDLVLRRPHIKSLVKQVIDQSS 1080 NDQIQRETPEKSNGKSIKKEKASNEKQN+VPKSKKSEREKSIEVPNKE EHSFSSDSHSEISDELLKEKITAILSGADLSKITLNQVCDGVVDQFPDVDLVLRRPHIKSLVKQVIDQSS Sbjct: 220 NDQIQRETPEKSNGKSIKKEKASNEKQNHVPKSKKSEREKSIEVPNKEVEHSFSSDSHSEISDELLKEKITAILSGADLSKITLNQVCDGVVDQFPDVDLVLRRPHIKSLVKQVIDQSS 338
BLAST of DEK_C domain-containing protein vs. Planmine SMEST
Match: SMESG000018659.1 (SMESG000018659.1) HSP 1 Score: 162.155 bits (409), Expect = 7.986e-46 Identity = 84/107 (78.50%), Postives = 89/107 (83.18%), Query Frame = 2 Query: 68 MENENVIESVNSDINNSREKRIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFDENDE 388 MENE+VIESVNSDIN SREK I K TFEMKSDRIIKPITGNGIKFG IKRIQD I RSKLNGELSKN++LLCYRT GKAGKRLS+IKKFNGFDE+DE Sbjct: 1 MENEDVIESVNSDINKSREKLIHK-----------TFEMKSDRIIKPITGNGIKFGYIKRIQDEILRSKLNGELSKNVYLLCYRTTGKAGKRLSAIKKFNGFDEDDE 96
BLAST of DEK_C domain-containing protein vs. Planmine SMEST
Match: SMESG000074552.1 (SMESG000074552.1) HSP 1 Score: 146.362 bits (368), Expect = 5.766e-42 Identity = 74/90 (82.22%), Postives = 78/90 (86.67%), Query Frame = 2 Query: 119 REKRIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFDENDE 388 REKRIRK TFE+KSDRIIKPITGNGIKFG +KRIQDAIHRSKLNGELSKN+HLLCYRTIGKAGKRLSSIKKFNGFDE+DE Sbjct: 31 REKRIRK-----------TFEIKSDRIIKPITGNGIKFGYVKRIQDAIHRSKLNGELSKNVHLLCYRTIGKAGKRLSSIKKFNGFDEDDE 109
BLAST of DEK_C domain-containing protein vs. Planmine SMEST
Match: SMESG000045951.1 (SMESG000045951.1) HSP 1 Score: 142.895 bits (359), Expect = 4.490e-39 Identity = 74/90 (82.22%), Postives = 76/90 (84.44%), Query Frame = 2 Query: 119 REKRIRKSVSRLSDVYDKTFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFDENDE 388 REKRIRK TFEMKSDRIIKPITGNGIKFG IKRIQDAIHRSKLNGELSKN+ LLCYRTIGKAGKRLSSIKKF GFDE+DE Sbjct: 125 REKRIRK-----------TFEMKSDRIIKPITGNGIKFGYIKRIQDAIHRSKLNGELSKNVKLLCYRTIGKAGKRLSSIKKFYGFDEDDE 203
BLAST of DEK_C domain-containing protein vs. Planmine SMEST
Match: SMESG000017493.1 (SMESG000017493.1) HSP 1 Score: 134.42 bits (337), Expect = 2.374e-38 Identity = 65/72 (90.28%), Postives = 68/72 (94.44%), Query Frame = 2 Query: 173 TFEMKSDRIIKPITGNGIKFGDIKRIQDAIHRSKLNGELSKNMHLLCYRTIGKAGKRLSSIKKFNGFDENDE 388 T EMKSD IIKPIT NGIKFGDIK+IQD+IHRSKLNGELSKN+HLLCYRTIGKAGKRLSSIKKFNGFDE DE Sbjct: 2 TLEMKSDSIIKPITCNGIKFGDIKQIQDSIHRSKLNGELSKNVHLLCYRTIGKAGKRLSSIKKFNGFDEVDE 73 The following BLAST results are available for this feature:
BLAST of DEK_C domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of DEK_C domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of DEK_C domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of DEK_C domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of DEK_C domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of DEK_C domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of DEK_C domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of DEK_C domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DEK_C domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of DEK_C domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of DEK_C domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of DEK_C domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of DEK_C domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of DEK_C domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009265 ID=SMED30009265|Name=DEK_C domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1288bpback to top protein sequence of SMED30009265-orf-1 >SMED30009265-orf-1 ID=SMED30009265-orf-1|Name=SMED30009265-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=236bp MENENVIESVNSDINNSREKRIRKSVSRLSDVYDKTFEMKSDRIIKPITGback to top protein sequence of SMED30009265-orf-2 >SMED30009265-orf-2 ID=SMED30009265-orf-2|Name=SMED30009265-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp MELSINFPMLTSCCAGRISNPLSNKLSINLPEYILDLCCHGNHQWAIRFYback to top Annotated Terms
The following terms have been associated with this transcript:
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