bcl2-3

Overview
Namebcl2-3 (AEX93482.1)
Smed IDSMED30009252
Length (bp)857
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of bcl2-3 (SMED30009252) t-SNE clustered cells

Violin plots show distribution of expression levels for bcl2-3 (SMED30009252) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of bcl2-3 (SMED30009252) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for bcl2-3 (SMED30009252) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009252

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30009252h1SMcG0009446 Contig6675GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009252h1SMcG0014949 Contig6675GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30009252h1SMcG0014949 dd_Smed_v4_2886_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30009252h1SMcG0014949 dd_Smed_v4_2886_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of bcl2-3 vs. Ensembl Human
Match: BAK1 (BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:HGNC:949])

HSP 1 Score: 52.373 bits (124), Expect = 1.497e-7
Identity = 36/163 (22.09%), Postives = 71/163 (43.56%), Query Frame = 2 
Query:   35 ASNEEILDIEEETRVIFECYLEDSLEKDKE-NNVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 520
            AS E++    EE   +F  Y+    ++++E   V  P  P   M  L          + ++L  I   ++   D     +L   ++    ++A+  F+++A  + ++ +NW  +V L  FGY +A      G   F   + ++++D      +  WI  +GGW
Sbjct:   22 ASEEQVAQDTEE---VFRSYVFYRHQQEQEAEGVAAPADP--EMVTLPLQPSSTMGQVGRQLAIIGDDINRRYDSEFQTMLQ--HLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGFLGQVTRFVVDFMLHHCIARWIAQRGGW 177
[Close]
BLAST of bcl2-3 vs. Ensembl Human
Match: BAK1 (BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:HGNC:949])

HSP 1 Score: 47.3654 bits (111), Expect = 5.848e-6
Identity = 19/73 (26.03%), Postives = 38/73 (52.05%), Query Frame = 2 
Query:  302 KSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 520
            ++A+  F+++A  + ++ +NW  +V L  FGY +A      G   F   + ++++D      +  WI  +GGW
Sbjct:   85 ENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGFLGQVTRFVVDFMLHHCIARWIAQRGGW 157
[Close]
BLAST of bcl2-3 vs. Ensembl Zebrafish
Match: zgc:153993 (zgc:153993 [Source:ZFIN;Acc:ZDB-GENE-060929-176])

HSP 1 Score: 55.8398 bits (133), Expect = 5.204e-9
Identity = 45/171 (26.32%), Postives = 76/171 (44.44%), Query Frame = 2 
Query:   35 ASNEEILD--IEEETRVIFECYLEDSLEKDKEN-NVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIET 538
               E+++D  I E++ V+   Y+      D  N +V      G P D+     KE    +V +LL IA   D+  +  L  +++ V  N     A + F  VA+ + D+ +NW  +V LF   Y +    L Q   +  K +  W +   ++  +  WI  QGGW G+  +
Sbjct:   19 TGGEDVVDDAIIEQSAVLLRGYVIQQATIDDPNIHVSHETLGGRPQDEEDPQIKE----VVDQLLKIAD--DLNKNAELQHLISTVQSN----CAQDVFMTVARSIFDDGINWGRVVALFHLAYRLIFQALTQNHLEIIKNIISWFLQFMKKH-ISAWIRQQGGWEGIFRS 178
[Close]
BLAST of bcl2-3 vs. Ensembl Xenopus
Match: ENSXETT00000052860.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:2:78680614:78766772:-1 gene:ENSXETG00000017363.1 transcript:ENSXETT00000052860.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.9802 bits (110), Expect = 5.685e-6
Identity = 21/60 (35.00%), Postives = 33/60 (55.00%), Query Frame = 2 
Query:  341 VLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 520
            + +N +NW  I+ L  FGY +A   +  G R FF+ + QW++       +  WI S+GGW
Sbjct:   77 LFENGINWGRILSLLGFGYRMAVYVVRNGQRGFFRIINQWMVRYVMESCIAQWISSRGGW 136
[Close]
BLAST of bcl2-3 vs. Ensembl Mouse
Match: Bak1 (BCL2-antagonist/killer 1 [Source:MGI Symbol;Acc:MGI:1097161])

HSP 1 Score: 49.2914 bits (116), Expect = 1.291e-6
Identity = 33/156 (21.15%), Postives = 67/156 (42.95%), Query Frame = 2 
Query:   56 DIEEETRVIFECYLEDSLEKDKEN-NVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 520
             + ++T  +F  Y+    ++++E      P  P   MD+L          + ++L  I   ++   D     +L  +    G  +A+  F+++A  +  + ++W  +V L  FGY +A     +G   F   +  ++ DI     +  WI  +GGW
Sbjct:   24 QVAQDTEEVFRSYVFYLHQQEQETQGAAAPANP--EMDNLPLEPNSILGQVGRQLALIGDDINRRYDTEFQNLLEQLQPTAG--NAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGFLGQVTCFLADIILHHYIARWIAQRGGW 175
[Close]
BLAST of bcl2-3 vs. UniProt/SwissProt
Match: sp|Q16611|BAK_HUMAN (Bcl-2 homologous antagonist/killer OS=Homo sapiens OX=9606 GN=BAK1 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 7.188e-7
Identity = 36/163 (22.09%), Postives = 71/163 (43.56%), Query Frame = 2 
Query:   35 ASNEEILDIEEETRVIFECYLEDSLEKDKE-NNVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 520
            AS E++    EE   +F  Y+    ++++E   V  P  P   M  L          + ++L  I   ++   D     +L   ++    ++A+  F+++A  + ++ +NW  +V L  FGY +A      G   F   + ++++D      +  WI  +GGW
Sbjct:   22 ASEEQVAQDTEE---VFRSYVFYRHQQEQEAEGVAAPADP--EMVTLPLQPSSTMGQVGRQLAIIGDDINRRYDSEFQTMLQ--HLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGFLGQVTRFVVDFMLHHCIARWIAQRGGW 177
[Close]
BLAST of bcl2-3 vs. UniProt/SwissProt
Match: sp|O08734|BAK_MOUSE (Bcl-2 homologous antagonist/killer OS=Mus musculus OX=10090 GN=Bak1 PE=1 SV=3)

HSP 1 Score: 49.2914 bits (116), Expect = 9.032e-6
Identity = 33/156 (21.15%), Postives = 67/156 (42.95%), Query Frame = 2 
Query:   56 DIEEETRVIFECYLEDSLEKDKEN-NVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 520
             + ++T  +F  Y+    ++++E      P  P   MD+L          + ++L  I   ++   D     +L  +    G  +A+  F+++A  +  + ++W  +V L  FGY +A     +G   F   +  ++ DI     +  WI  +GGW
Sbjct:   24 QVAQDTEEVFRSYVFYLHQQEQETQGAAAPANP--EMDNLPLEPNSILGQVGRQLALIGDDINRRYDTEFQNLLEQLQPTAG--NAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGFLGQVTCFLADIILHHYIARWIAQRGGW 175
[Close]
BLAST of bcl2-3 vs. TrEMBL
Match: H2DL20 (Bcl2-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 358.992 bits (920), Expect = 4.253e-123
Identity = 200/202 (99.01%), Postives = 202/202 (100.00%), Query Frame = 2 
Query:   32 MASNEEILDIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHMNSHXXXXXXXXXXXXXXXIIGFVWFRSKSAQ 637
            MASNEEI+DIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDI+SDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHMNSHVVGAVTVVSVVAVVAIIGFVWFRSKSAQ
Sbjct:    1 MASNEEIIDIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIKSDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHMNSHVVGAVTVVSVVAVVAIIGFVWFRSKSAQ 202
[Close]
BLAST of bcl2-3 vs. TrEMBL
Match: H2DL12 (Bak-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 4.575e-38
Identity = 71/157 (45.22%), Postives = 104/157 (66.24%), Query Frame = 2 
Query:   65 EETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDL--TTNDKEAYA-IIVQKLLSIAGQM--DIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 520
            EET  IFEC++E  LE D+E N IDPGTPG+P +DL  T ++++  +  I +KL  IA  +  D ES  ++ +++ +VN+      AF++FSE+A++V+ N + W  +  LF FGY IARDRL QG + F   L +WI+DIF+R  +F WIL+ GGW
Sbjct:   17 EETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGW 173
[Close]
BLAST of bcl2-3 vs. TrEMBL
Match: A0A1I8J8Q2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030130g1 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.631e-13
Identity = 46/166 (27.71%), Postives = 80/166 (48.19%), Query Frame = 2 
Query:   56 DIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTNDK-EAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNIN-EGFKSAFNDFSEVAQRVL-DNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHM 544
            ++  +T  +F+ ++ +   +D+E   + PG P  P   L   D     A I ++L  I  ++     P   +++  ++       S+F+ F  VA+R+  D  +NW  IV LF FGY IA +++ +G   F + + QWI     +  +  WI   GGW  +  THM
Sbjct:   62 NVRRQTDTVFQAFVFERYTRDREQGRLQPGAPSSPELILPPADPLSVEAGIGRRLAEIGDEIQQRYQPEFDEMIKSLDGQLTDPASSFDTFRRVARRLFHDGSINWGRIVALFCFGYRIAVEKIRRGVSGFLRNVVQWIFRFLVQERIARWIADHGGWRSV--THM 225
[Close]
BLAST of bcl2-3 vs. TrEMBL
Match: A0A267FYD8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030621g2 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 5.396e-13
Identity = 46/166 (27.71%), Postives = 80/166 (48.19%), Query Frame = 2 
Query:   56 DIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTNDK-EAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNIN-EGFKSAFNDFSEVAQRVL-DNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHM 544
            ++  +T  +F+ ++ +   +D+E   + PG P  P   L   D     A I ++L  I  ++     P   +++  ++       S+F+ F  VA+R+  D  +NW  IV LF FGY IA +++ +G   F + + QWI     +  +  WI   GGW  +  THM
Sbjct:   86 NVRRQTDTVFQAFVFERYTRDREQGRLQPGAPSSPELILPPADPLSVEAGIGRRLAEIGDEIQQRYQPEFDEMIKSLDGQLTDPASSFDTFRRVARRLFHDGSINWGRIVALFCFGYRIAVEKIRRGVSGFLRNVVQWIFRFLVQERIARWIADHGGWRSV--THM 249
[Close]
BLAST of bcl2-3 vs. TrEMBL
Match: W4YE49 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.562e-10
Identity = 50/194 (25.77%), Postives = 94/194 (48.45%), Query Frame = 2 
Query:   56 DIEEETRVIFECYLEDSLEKDKEN--NVIDPGTPGIP-MDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHMNSHXXXXXXXXXXXXXXXIIGFVWFRSK 628
            ++ E+T +I   +    L  D ++  N I+  TP IP + + T +     + + ++L  I  Q+D + +    +++ M++I     +A+  F+ VA+R+    +NW  I  L  FGY IARD ++    DFF  +   ++       +  WI  QGGW   +   + +  VG + +  + A+  +   VW RS+
Sbjct:   19 NVIEQTEMIVRNFFHQRLAMDMQDQTNDINISTPRIPELQNFTADPLSPTSRVGRRLAQIGDQIDAQYEGEFRQMIQMLHITP--STAYQAFAGVARRLFIKGINWGRISALLMFGYRIARD-VMTSVGDFFHKIVGDLVRFIISERIATWIAQQGGWISALNFRIENPGVGFIAIAGLSALAIVCAVVWSRSR 209
[Close]
BLAST of bcl2-3 vs. Ensembl Cavefish
Match: zgc:153993 (apoptosis regulator BAX-like [Source:NCBI gene;Acc:103047816])

HSP 1 Score: 55.8398 bits (133), Expect = 5.882e-9
Identity = 35/120 (29.17%), Postives = 58/120 (48.33%), Query Frame = 2 
Query:  179 NDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIET 538
             D     ++V++LL IA   D+  +  L  +++ V  N     A + F  VA+ +  + +NW  IV LF   Y +  + L Q   +  K +  W++   R+  V  WI  QGGW G+I +
Sbjct:   66 GDDPQIKVVVEQLLRIAD--DLNRNTELQHLISTVQAN----CAQDVFMTVARTIFTDGINWGRIVALFHLAYQLIYNALTQNHLEIIKNIISWVLQYIRQH-VSAWIRQQGGWEGVIRS 178
[Close]
BLAST of bcl2-3 vs. Ensembl Nematostella
Match: EDO39531 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9S4])

HSP 1 Score: 54.6842 bits (130), Expect = 2.253e-9
Identity = 33/122 (27.05%), Postives = 59/122 (48.36%), Query Frame = 2 
Query:  173 TTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIET 538
            T N + + ++  + +  ++ +++    P+L  + N +N+     SA   F +VA  V  + +NW  IV +F FG  +A+     G  D  + +  W+ D     S  +WI +QGGW    ET
Sbjct:   34 TGNGRISTSLTARNMRRLSDELEERHGPLLCNMCNSLNMTA--NSAHEKFVQVADEVFKDGINWGRIVAVFTFGGRLAQFCARNGLHDGVREVEAWLGDYIANLS--HWIQTQGGWVCTFET 151
[Close]
BLAST of bcl2-3 vs. Ensembl Nematostella
Match: EDO49892 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF38])

HSP 1 Score: 53.9138 bits (128), Expect = 6.751e-9
Identity = 27/119 (22.69%), Postives = 63/119 (52.94%), Query Frame = 2 
Query:  179 NDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQG-ARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLI 532
              ++  A I ++L  +  ++D +     + ++  +++  G  +A++ F+ VA+R+  + ++W  I+ L  FGY IA   + +G +  F + + ++++D   R  +  WI   GGW  ++
Sbjct:   71 TSRQHIATIGRRLGEVGDEIDAKYRDQFHDMIESLHLTPG--TAYDTFAAVARRLFRSGISWGRIIALLCFGYEIAITVIRRGFSGSFLRRIIRFVVDFIFRERIARWIAEHGGWVAVM 187
[Close]
BLAST of bcl2-3 vs. Ensembl Nematostella
Match: EDO37464 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFS9])

HSP 1 Score: 49.2914 bits (116), Expect = 2.248e-7
Identity = 30/110 (27.27%), Postives = 55/110 (50.00%), Query Frame = 2 
Query:  206 VQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIE 535
             + L  +A +++ +   +L  + + +NI+    +A++ F E+A  V  + VNW  IV L+ FG  +A        +D  + +  W+       S   WI ++GGW+GL E
Sbjct:   42 AETLRKLADKIEEQYPALLNHLCHKLNISR--STAYSTFVEIAGEVFADGVNWGRIVVLYAFGGRLAVYCEQHNMKDLVQSVTDWVGKYIAGLS--EWIANEGGWDGLKE 147
[Close]
BLAST of bcl2-3 vs. Ensembl Nematostella
Match: EDO33714 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRF5])

HSP 1 Score: 47.3654 bits (111), Expect = 2.996e-7
Identity = 28/105 (26.67%), Postives = 48/105 (45.71%), Query Frame = 2 
Query:  215 LLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGL 529
            L S+A  M+ E   +       + I     +A + F  VA+ +    +NW  IV LF FG ++A   +     +   ++ +WI   F   ++ +WI+  GGW  L
Sbjct:    1 LRSLASHMEAEYPDLFETACTKLGITPA--TARSTFFSVAKEIFQTGINWGRIVALFTFGGVVAHHFIESERPEMVPHVVEWIPS-FIADNLLSWIMENGGWVSL 102
[Close]
BLAST of bcl2-3 vs. Ensembl Nematostella
Match: EDO42793 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0T4])

HSP 1 Score: 46.595 bits (109), Expect = 5.901e-7
Identity = 28/76 (36.84%), Postives = 40/76 (52.63%), Query Frame = 2 
Query:  305 SAFNDFSEVAQRVL-DNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGL 529
            +AF+ F  VA ++  D  +NW  IV LF FGY +A   L Q      K + +W++  F +  +  WI  QGGW  L
Sbjct:   31 TAFDTFLAVALQIFQDGVINWGRIVTLFYFGYKLALKVLNQ--IPLIKMIIEWVVK-FVKEKLVKWIFQQGGWVSL 103
[Close]
BLAST of bcl2-3 vs. Ensembl Medaka
Match: zgc:153993 (apoptosis regulator BAX [Source:NCBI gene;Acc:101168687])

HSP 1 Score: 53.9138 bits (128), Expect = 5.245e-8
Identity = 33/123 (26.83%), Postives = 64/123 (52.03%), Query Frame = 2 
Query:  173 TTNDKEAYAI--IVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIE 535
            + N++E   I  +V++LL IA   DI  + VL +++N  + +     A + F EVA+ +  + +NW  +V LF   Y      L     +  + +  W++ + +   ++ W++ QGGW G+I+
Sbjct:  151 SPNEQEDPQIKEVVEQLLKIAD--DINRNAVLQQLINQASGS----GAQDIFMEVARSIFADGINWGRVVALFHLAYKFIYKALTSNHLETIRMIISWVLQVIKEV-LYPWLVQQGGWVGVIQ 266
[Close]
BLAST of bcl2-3 vs. Planmine SMEST
Match: SMESG000011963.1 (SMESG000011963.1)

HSP 1 Score: 380.178 bits (975), Expect = 5.968e-135
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 2 
Query:   32 MASNEEILDIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHMNSHXXXXXXXXXXXXXXXIIGFVWFRSKSAQ 637
            MASNEEILDIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHMNSHVVGAVTVVSVVAVVAIIGFVWFRSKSAQ
Sbjct:    1 MASNEEILDIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTNDKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGWNGLIETHMNSHVVGAVTVVSVVAVVAIIGFVWFRSKSAQ 202
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BLAST of bcl2-3 vs. Planmine SMEST
Match: SMESG000011981.1 (SMESG000011981.1)

HSP 1 Score: 142.895 bits (359), Expect = 1.272e-41
Identity = 71/157 (45.22%), Postives = 104/157 (66.24%), Query Frame = 2 
Query:   65 EETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDL--TTNDKEAYA-IIVQKLLSIAGQM--DIESDPVLYKVLNMVNINEGFKSAFNDFSEVAQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRRFSVFNWILSQGGW 520
            EET  IFEC++E  LE D+E N IDPGTPG+P +DL  T ++++  +  I +KL  IA  +  D ES  ++ +++ +VN+      AF++FSE+A++V+ N + W  +  LF FGY IARDRL QG + F   L +WI+DIF+R  +F WIL+ GGW
Sbjct:   17 EETIEIFECFVETQLENDRERNRIDPGTPGLPPEDLPHTPDNRDNVSWQIGKKLAGIADTLVLDEESRGLISEIVQLVNVGPDDTRAFDEFSEIAKKVIANEITWENVATLFYFGYCIARDRLTQGVQHFLSQLLKWIVDIFKRLGIFQWILNNGGW 173
The following BLAST results are available for this feature:
BLAST of bcl2-3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
BAK11.497e-722.09BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:H... [more]
BAK15.848e-626.03BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of bcl2-3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1539935.204e-926.32zgc:153993 [Source:ZFIN;Acc:ZDB-GENE-060929-176][more]
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BLAST of bcl2-3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000052860.15.685e-635.00pep primary_assembly:Xenopus_tropicalis_v9.1:2:786... [more]
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BLAST of bcl2-3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Bak11.291e-621.15BCL2-antagonist/killer 1 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of bcl2-3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q16611|BAK_HUMAN7.188e-722.09Bcl-2 homologous antagonist/killer OS=Homo sapiens... [more]
sp|O08734|BAK_MOUSE9.032e-621.15Bcl-2 homologous antagonist/killer OS=Mus musculus... [more]
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BLAST of bcl2-3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2DL204.253e-12399.01Bcl2-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV=... [more]
H2DL124.575e-3845.22Bak-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
A0A1I8J8Q23.631e-1327.71Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FYD85.396e-1327.71Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
W4YE493.562e-1025.77Uncharacterized protein OS=Strongylocentrotus purp... [more]
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BLAST of bcl2-3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1539935.882e-929.17apoptosis regulator BAX-like [Source:NCBI gene;Acc... [more]
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BLAST of bcl2-3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO395312.253e-927.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO498926.751e-922.69Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO374642.248e-727.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO337142.996e-726.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427935.901e-736.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of bcl2-3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1539935.245e-826.83apoptosis regulator BAX [Source:NCBI gene;Acc:1011... [more]
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BLAST of bcl2-3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000011963.15.968e-135100.00SMESG000011963.1[more]
SMESG000011981.11.272e-4145.22SMESG000011981.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30009252
GenBankAEX93482.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009252 ID=SMED30009252|Name=bcl2-3|organism=Schmidtea mediterranea sexual|type=transcript|length=857bp
CTATATAAATCTTATATGATAAATCTATAAAATGGCTAGTAATGAGGAAA
TACTTGATATTGAAGAAGAAACTAGAGTAATATTTGAGTGTTATTTAGAA
GACAGTCTTGAAAAAGACAAAGAAAATAATGTTATTGATCCAGGAACACC
AGGAATTCCAATGGACGATCTAACGACTAATGACAAAGAAGCTTATGCAA
TTATCGTGCAAAAATTGTTATCTATTGCAGGTCAAATGGATATAGAGAGT
GATCCAGTGTTGTACAAAGTTTTAAATATGGTTAATATAAATGAAGGATT
CAAGTCGGCTTTTAATGATTTCTCTGAAGTGGCACAAAGAGTTCTTGATA
ACCACGTTAATTGGAACGCAATCGTATACCTGTTTCGATTTGGTTATTTA
ATAGCCCGAGATAGATTGGTTCAAGGAGCTCGAGATTTTTTTAAATATCT
CTGTCAATGGATCATAGACATTTTCCGTAGATTTTCAGTATTTAATTGGA
TATTGAGTCAAGGAGGCTGGAATGGATTGATAGAAACGCACATGAATTCG
CATGTTGTCGGAGCTGTTACTGTTGTCAGCGTCGTTGCAGTAGTAGCGAT
CATTGGATTTGTATGGTTCAGATCAAAAAGTGCTCAATGAGATCCCAATA
TTTTTATCAGTATTTCTGAAAATCAACATGTGGATTTTAGCTTACTTTTG
AATAGTTTAACACATTTTTATATGTTTTCCATCGATGTTTGGTGAATTTA
TATCAGTTTTACTCATTTATTTTCTACGCAACCATTTTCGAATCCAGACA
TTTCTAAGTTGTCAAATTTTTTATTAAAAATAAATTCCCAGAAGTCTGAA
AAAAAAA
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protein sequence of SMED30009252-orf-1

>SMED30009252-orf-1 ID=SMED30009252-orf-1|Name=SMED30009252-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=203bp
MASNEEILDIEEETRVIFECYLEDSLEKDKENNVIDPGTPGIPMDDLTTN
DKEAYAIIVQKLLSIAGQMDIESDPVLYKVLNMVNINEGFKSAFNDFSEV
AQRVLDNHVNWNAIVYLFRFGYLIARDRLVQGARDFFKYLCQWIIDIFRR
FSVFNWILSQGGWNGLIETHMNSHVVGAVTVVSVVAVVAIIGFVWFRSKS
AQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR036834Blc2-like_sf
IPR026298Blc2_fam
IPR020726Bcl2_BH2_motif_CS
IPR002475Bcl2-like
Vocabulary: biological process
TermDefinition
GO:0042981regulation of apoptotic process
GO:0043065positive regulation of apoptotic process
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026298Blc2 familyPFAMPF00452Bcl-2coord: 64..163
e-value: 1.7E-12
score: 47.8
IPR026298Blc2 familyPANTHERPTHR11256BCL-2 RELATEDcoord: 9..192
IPR036834Blc2-like superfamilyGENE3DG3DSA:1.10.437.10coord: 2..169
e-value: 2.6E-29
score: 104.5
IPR036834Blc2-like superfamilySUPERFAMILYSSF56854Bcl-2 inhibitors of programmed cell deathcoord: 10..194
NoneNo IPR availablePANTHERPTHR11256:SF56coord: 9..192
NoneNo IPR availableTMHMMTMhelixcoord: 174..196
IPR020726Apoptosis regulator, Bcl-2, BH2 motif, conserved sitePROSITEPS01258BH2coord: 156..167
IPR002475Bcl2-likePROSITEPS50062BCL2_FAMILYcoord: 62..165
score: 13.724