DNA topoisomerase 2 binding protein 1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30009244 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Human
Match: TOPBP1 (DNA topoisomerase II binding protein 1 [Source:HGNC Symbol;Acc:HGNC:17008]) HSP 1 Score: 130.568 bits (327), Expect = 1.087e-29 Identity = 79/277 (28.52%), Postives = 137/277 (49.46%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFE---------DVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F KA +S+ EF+ + +A+K+ EN S ++ F G F++LK L I GP ++++C+ +P+ P++N + + IS + L+ +++++ ++MM G S+ L+ V S KY A N +L P WI +W E + + ++ FK PIF IC++G +R ++ + +GG + G+L NE THLI + K +KY A +WNV V W DS++ G+C++ + Y+ P
Sbjct: 20 SKCFFKALESIKEFQSEEYLQIITEEEALKIKEN----------DRSLYICDPFSGVVFDHLKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTDINMEDFKCPIFLGCIICVTGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDESIYKTEP 286 HSP 2 Score: 113.235 bits (282), Expect = 2.170e-24 Identity = 69/177 (38.98%), Postives = 96/177 (54.24%), Query Frame = 2
Query: 3386 LFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGT-NTLIAANDRDDLLPLALCAKRWR-------EFG--GKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
+F L+ +E +I +LGG V+E D TH++ RN+K+L++VA+GKW++H SY+EAC ++ E YEWGSS + L N + LAL A RWR E G AF W++IL +S+ FKR+ SGG VLP G S+P E TH F +LN
Sbjct: 1269 IFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGSSSILDVLTGINVQQRR--LALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLP-GHSVPLFKEATHLFSDLN 1442 HSP 3 Score: 67.0106 bits (162), Expect = 2.463e-10 Identity = 58/201 (28.86%), Postives = 93/201 (46.27%), Query Frame = 2
Query: 2183 SNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
S T +GLF+ F+ S+ + S + II E +G+I S + V + C E+ ++VT ++ CI + D + LF P V+ G+ P L CVIS E + L + LGA+VQ+ ++ K + AST ++ K+ + ++ W++P V WLLE + AD S FLI
Sbjct: 545 STITEEGLFSQ-KSFLVLGFSNENESNIANIIKENAGKIMSLLSRT---VADYAVVPLLGCEVEATVGEVVTNTWLVTCIDYQTLFDPKSNPLFTPVPVMTGMTP---LEDCVISFSQCAGAEKESLTFLANLLGASVQEYFVRKSNAKKGMFASTHLILKERGGSKYEAAKKWNLPAVTIAWLLETARTGKRADESHFLI 738
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Human
Match: TOPBP1 (DNA topoisomerase II binding protein 1 [Source:HGNC Symbol;Acc:HGNC:17008]) HSP 1 Score: 130.568 bits (327), Expect = 1.141e-29 Identity = 79/277 (28.52%), Postives = 137/277 (49.46%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFE---------DVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F KA +S+ EF+ + +A+K+ EN S ++ F G F++LK L I GP ++++C+ +P+ P++N + + IS + L+ +++++ ++MM G S+ L+ V S KY A N +L P WI +W E + + ++ FK PIF IC++G +R ++ + +GG + G+L NE THLI + K +KY A +WNV V W DS++ G+C++ + Y+ P
Sbjct: 20 SKCFFKALESIKEFQSEEYLQIITEEEALKIKEN----------DRSLYICDPFSGVVFDHLKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTDINMEDFKCPIFLGCIICVTGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDESIYKTEP 286 HSP 2 Score: 113.235 bits (282), Expect = 2.203e-24 Identity = 69/177 (38.98%), Postives = 96/177 (54.24%), Query Frame = 2
Query: 3386 LFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGT-NTLIAANDRDDLLPLALCAKRWR-------EFG--GKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
+F L+ +E +I +LGG V+E D TH++ RN+K+L++VA+GKW++H SY+EAC ++ E YEWGSS + L N + LAL A RWR E G AF W++IL +S+ FKR+ SGG VLP G S+P E TH F +LN
Sbjct: 1264 IFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGSSSILDVLTGINVQQRR--LALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLP-GHSVPLFKEATHLFSDLN 1437 HSP 3 Score: 67.0106 bits (162), Expect = 2.524e-10 Identity = 58/201 (28.86%), Postives = 93/201 (46.27%), Query Frame = 2
Query: 2183 SNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
S T +GLF+ F+ S+ + S + II E +G+I S + V + C E+ ++VT ++ CI + D + LF P V+ G+ P L CVIS E + L + LGA+VQ+ ++ K + AST ++ K+ + ++ W++P V WLLE + AD S FLI
Sbjct: 540 STITEEGLFSQ-KSFLVLGFSNENESNIANIIKENAGKIMSLLSRT---VADYAVVPLLGCEVEATVGEVVTNTWLVTCIDYQTLFDPKSNPLFTPVPVMTGMTP---LEDCVISFSQCAGAEKESLTFLANLLGASVQEYFVRKSNAKKGMFASTHLILKERGGSKYEAAKKWNLPAVTIAWLLETARTGKRADESHFLI 733
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Human
Match: SLF1 (SMC5-SMC6 complex localization factor 1 [Source:HGNC Symbol;Acc:HGNC:25408]) HSP 1 Score: 67.3958 bits (163), Expect = 5.943e-12 Identity = 33/81 (40.74%), Postives = 49/81 (60.49%), Query Frame = 2
Query: 3395 LTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWG 3637
+TGF+ E L ++ +L ++S + THLI RL +++KFL+A A+GKWI+ YI + RWL+ET YEWG
Sbjct: 12 MTGFKMEEKEALVKLLLKLDCTFIKSEKY-KNCTHLIAERLCKSEKFLAACAAGKWILTKDYIIHSAKSGRWLDETTYEWG 91
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Human
Match: SLF1 (SMC5-SMC6 complex localization factor 1 [Source:HGNC Symbol;Acc:HGNC:25408]) HSP 1 Score: 65.855 bits (159), Expect = 6.484e-12 Identity = 39/109 (35.78%), Postives = 56/109 (51.38%), Query Frame = 2
Query: 3395 LTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE 3721
+TGF+ E L ++ +L ++S + THLI RL +++KFL+A A+GKWI+ YI + RWL+ET YEWG D + KRWRE
Sbjct: 12 MTGFKMEEKEALVKLLLKLDCTFIKSEKY-KNCTHLIAERLCKSEKFLAACAAGKWILTKDYIIHSAKSGRWLDETTYEWGYK------IEKDSRYSPQMQSAPKRWRE 113
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Human
Match: SLF1 (SMC5-SMC6 complex localization factor 1 [Source:HGNC Symbol;Acc:HGNC:25408]) HSP 1 Score: 67.0106 bits (162), Expect = 2.379e-10 Identity = 33/81 (40.74%), Postives = 49/81 (60.49%), Query Frame = 2
Query: 3395 LTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWG 3637
+TGF+ E L ++ +L ++S + THLI RL +++KFL+A A+GKWI+ YI + RWL+ET YEWG
Sbjct: 12 MTGFKMEEKEALVKLLLKLDCTFIKSEKY-KNCTHLIAERLCKSEKFLAACAAGKWILTKDYIIHSAKSGRWLDETTYEWG 91
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Celegans
Match: mus-101 (pep chromosome:WBcel235:I:10479283:10484969:-1 gene:WBGene00009507.1 transcript:F37D6.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mus-101) HSP 1 Score: 83.1889 bits (204), Expect = 1.054e-15 Identity = 67/257 (26.07%), Postives = 128/257 (49.81%), Query Frame = 2
Query: 116 FEDVKAVKLSENFIMSN-YEKLE-SNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVE-SPKYSSAHNNLITVLSPDWIYDIW-NSQIENDPF---NEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQW-NVKIVCINWLHDSLKDGYCKEYNKY 862
F+ KAV + +I S+ E L+ S FV F+G+ F L++ + ++GP I+L + LP+ P+++ ++ KIS + L++ +KQ+L I M G + + L+T E + KY +A NN I ++ WI D+W SQ F + + + + +F L++ ++ +R +I ++GG G ++ ++LI+ KY+ A++W +++IV W+ + G+ + KY
Sbjct: 57 FKTAKAVGIMPEWIDSDALEDLQKSEDFFVLPCFRGKLFRKLQARKLKVYGPPIVLESIEDGKQLPQWNHPVYSSVFQDVKISFTGLNLTKKQELYEKIGWMCGVVGDALYHETTHLVTEKAEQTEKYKAAVNNSIKLMRIGWIDDLWETSQTTMGRFSALSRDSVNSYALRVFEGLEMAITSIDGADRTNFIQLIEDHGGKVPGTMSKTRCSYLISDKITGVKYAKAVEWKSMQIVQSRWIRKCVDLGHLVDAGKY 313 HSP 2 Score: 62.3882 bits (150), Expect = 2.819e-9 Identity = 45/177 (25.42%), Postives = 74/177 (41.81%), Query Frame = 2
Query: 3380 PRLFL-LTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLP----------LALCAKRWREFGGK---------------AFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPI 3832
PR L ++ + L + I +LGG + ++ THLI + +QR K L ++A+GKW + Y+ RWL+E +EW L+A + +++ L LA WR+ + AF DWR ++ + + F I +GG V I
Sbjct: 945 PRFLLSVSNMDPQRAADLQETIMKLGGTIERE--FNKDVTHLIASNMQRAPKVLCSIAAGKWCLTPDYVTKSAEVGRWLDEKSFEWTR---EKLLACSKKNESLKERDSRKVSENLAAVCGLWRQRVAEMPVTFSVSMENRQNGAFSDWRCVIHLDDKRAGVFSSILEAGGAIVHSI 1116
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Fly
Match: mus101 (gene:FBgn0002878 transcript:FBtr0073817) HSP 1 Score: 103.99 bits (258), Expect = 7.140e-22 Identity = 65/257 (25.29%), Postives = 128/257 (49.81%), Query Frame = 2
Query: 125 VKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWN----SQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISK--SRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPN 877
++ +K SE + + L FV ++F+GE FE L+ I GP ++ C+ ++ +P+ +++ +++ ++S++ + +K++L LI M G Y + F + L++ ++S KY A N + V+ DW+ +W+ SQ E + ++++PIFF I SG ++ + ++N+NGGI+ +IT+ +K + KY A+++ ++ W+ DS GY YE P
Sbjct: 51 IRQIKPSEGYPLIAAGNLTKKDVFVLTQFEGEFFEQLQQTRALILGPPCLITCLRRNEPIPEGSSAIYSTAMRDLQVSATGITPQKKEELSRLINWMGGIYFQSFGHRTTHLISNTIKSSKYEQATLNGVPVMHVDWVQYVWDQSRRSQREGIMATDPDFDKYRLPIFFGANITCSGLDVARKDQVMRLVNDNGGIYHRAFRSQVVDIVITEQTKTDTEKYKAAIRYKKDVLLPEWIFDSCNRGYALPTKDYEVRPG 307 HSP 2 Score: 92.4337 bits (228), Expect = 2.128e-18 Identity = 59/177 (33.33%), Postives = 89/177 (50.28%), Query Frame = 2
Query: 3380 PRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLL--DRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWR----EFGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSL---PPVNEVTHSFINL 3883
P + G D + +L I +LGG+V E NL+ D THL+C R R +K L+ +A+GKWI++ YIE H +L+ETLYEWG+ L ++ P+A RWR GG AF D R+IL +E + + +GG +L + P +H F+++
Sbjct: 1208 PCFSISCGDDDEKRAELIARITQLGGKVCE-NLVNYDDSCTHLLCERPNRGEKMLACIAAGKWILNIQYIEQSHARGDFLDETLYEWGNPKAINLPTLAPEEE--PIAAAVHRWRTELSACGGGAFSDHRVILSMNERSGAPIRNVLRAGGACILEPTTPFSKDPVAKSASHCFVDV 1381
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Fly
Match: pbl (gene:FBgn0003041 transcript:FBtr0112817) HSP 1 Score: 65.855 bits (159), Expect = 2.238e-10 Identity = 38/154 (24.68%), Postives = 68/154 (44.16%), Query Frame = 2
Query: 194 FVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEK-QKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQ--IENDPFNEQYLKRFKIPIFFNLKICLSGF 646
+V +F+G +FE + + I GP + Y +L + P++N ++ + + ++ KL+NLI M G + L+ + KY A +TV+ P W++ W + +E D E + K ++ F KIC GF
Sbjct: 113 YVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGF 266
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Fly
Match: pbl (gene:FBgn0003041 transcript:FBtr0076771) HSP 1 Score: 65.855 bits (159), Expect = 2.400e-10 Identity = 38/154 (24.68%), Postives = 68/154 (44.16%), Query Frame = 2
Query: 194 FVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEK-QKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQ--IENDPFNEQYLKRFKIPIFFNLKICLSGF 646
+V +F+G +FE + + I GP + Y +L + P++N ++ + + ++ KL+NLI M G + L+ + KY A +TV+ P W++ W + +E D E + K ++ F KIC GF
Sbjct: 66 YVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGF 219
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Fly
Match: pbl (gene:FBgn0003041 transcript:FBtr0076772) HSP 1 Score: 63.929 bits (154), Expect = 1.042e-9 Identity = 39/154 (25.32%), Postives = 67/154 (43.51%), Query Frame = 2
Query: 194 FVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVM-EKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW--NSQIENDPFNEQYLKRFKIPIFFNLKICLSGF 646
+V +F+G +FE + + I GP + Y +L + P++N ++ + + E KL+NLI M G + L+ + KY A +TV+ P W++ W + +E D E + K ++ F KIC GF
Sbjct: 66 YVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGF 219
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Fly
Match: pbl (gene:FBgn0003041 transcript:FBtr0076773) HSP 1 Score: 53.9138 bits (128), Expect = 9.989e-7 Identity = 33/136 (24.26%), Postives = 58/136 (42.65%), Query Frame = 2
Query: 248 RTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEK-QKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQ--IENDPFNEQYLKRFKIPIFFNLKICLSGF 646
+ I GP + Y +L + P++N ++ + + ++ KL+NLI M G + L+ + KY A +TV+ P W++ W + +E D E + K ++ F KIC GF
Sbjct: 13 KLILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGF 148
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Zebrafish
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-060626-1]) HSP 1 Score: 129.413 bits (324), Expect = 1.683e-29 Identity = 73/268 (27.24%), Postives = 139/268 (51.87%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYL--KRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKY 862
N S + +AY +++E + K V++ + + S FVFS+F AF++ + L I P ++L+C+ + +PK P++N + + +S + LD + ++++LI++M G S+ L+ V S KY A + +L P W+ W ++ F+ L + ++ P+ +C++G ER ++ + + NG + G+L NE THLI +KY +A +WNV V ++WL DS++ G+C++ ++Y
Sbjct: 17 NGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDH-NDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKS-QDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRY 282 HSP 2 Score: 110.538 bits (275), Expect = 9.838e-24 Identity = 70/177 (39.55%), Postives = 96/177 (54.24%), Query Frame = 2
Query: 3380 PRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGT-NTLIAANDRDDLLPLALCAKRWRE-FGG-----KAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGS-SLPPVNEVTHSFINLN 3886
P FLL+ +E S +I LGG VLE D TH+I RN+K+L+A+A+GKWI+H SY+EAC E ++ E YEWGSS L + N + LAL A RWR+ G AF W ++L +++ F+R+ SGG VLP S SL E TH F++ +
Sbjct: 1270 PPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKR--LALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSL--FKETTHLFVDFS 1442 HSP 3 Score: 68.1662 bits (165), Expect = 7.641e-11 Identity = 52/201 (25.87%), Postives = 95/201 (47.26%), Query Frame = 2
Query: 2183 SNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPTVV-AGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
+ ++ GLF G FI + ++L +++ E +G+I SR + A + C E+ ++ T+ +++ C+ +L + LF P V G +P L CV+S+ F E D L + + LGA+VQD ++ + K + AST +V + ++ W +P V W+LE + AD ++L+
Sbjct: 533 AEASEGGLFFGKR-FILVGFGAEAEAQLSELVMENAGKILVG--RSRAVAHYAIVP-LLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSP---LKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLV 726
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Zebrafish
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-060626-1]) HSP 1 Score: 129.413 bits (324), Expect = 1.683e-29 Identity = 73/268 (27.24%), Postives = 139/268 (51.87%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYL--KRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKY 862
N S + +AY +++E + K V++ + + S FVFS+F AF++ + L I P ++L+C+ + +PK P++N + + +S + LD + ++++LI++M G S+ L+ V S KY A + +L P W+ W ++ F+ L + ++ P+ +C++G ER ++ + + NG + G+L NE THLI +KY +A +WNV V ++WL DS++ G+C++ ++Y
Sbjct: 17 NGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDH-NDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKS-QDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRY 282 HSP 2 Score: 110.538 bits (275), Expect = 9.838e-24 Identity = 70/177 (39.55%), Postives = 96/177 (54.24%), Query Frame = 2
Query: 3380 PRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGT-NTLIAANDRDDLLPLALCAKRWRE-FGG-----KAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGS-SLPPVNEVTHSFINLN 3886
P FLL+ +E S +I LGG VLE D TH+I RN+K+L+A+A+GKWI+H SY+EAC E ++ E YEWGSS L + N + LAL A RWR+ G AF W ++L +++ F+R+ SGG VLP S SL E TH F++ +
Sbjct: 1270 PPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKR--LALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSL--FKETTHLFVDFS 1442 HSP 3 Score: 68.1662 bits (165), Expect = 7.641e-11 Identity = 52/201 (25.87%), Postives = 95/201 (47.26%), Query Frame = 2
Query: 2183 SNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPTVV-AGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
+ ++ GLF G FI + ++L +++ E +G+I SR + A + C E+ ++ T+ +++ C+ +L + LF P V G +P L CV+S+ F E D L + + LGA+VQD ++ + K + AST +V + ++ W +P V W+LE + AD ++L+
Sbjct: 533 AEASEGGLFFGKR-FILVGFGAEAEAQLSELVMENAGKILVG--RSRAVAHYAIVP-LLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSP---LKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLV 726
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Zebrafish
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-060626-1]) HSP 1 Score: 129.413 bits (324), Expect = 1.775e-29 Identity = 73/268 (27.24%), Postives = 139/268 (51.87%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYL--KRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKY 862
N S + +AY +++E + K V++ + + S FVFS+F AF++ + L I P ++L+C+ + +PK P++N + + +S + LD + ++++LI++M G S+ L+ V S KY A + +L P W+ W ++ F+ L + ++ P+ +C++G ER ++ + + NG + G+L NE THLI +KY +A +WNV V ++WL DS++ G+C++ ++Y
Sbjct: 17 NGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDH-NDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKS-QDSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRY 282 HSP 2 Score: 107.842 bits (268), Expect = 6.967e-23 Identity = 70/184 (38.04%), Postives = 96/184 (52.17%), Query Frame = 2
Query: 3380 PRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGT-NTLIAANDRDDLLPLALCAKRWRE-FGG------------KAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGS-SLPPVNEVTHSFINLN 3886
P FLL+ +E S +I LGG VLE D TH+I RN+K+L+A+A+GKWI+H SY+EAC E ++ E YEWGSS L + N + LAL A RWR+ G AF W ++L +++ F+R+ SGG VLP S SL E TH F++ +
Sbjct: 1269 PPRFLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWGSSSILEALPSINSQQKR--LALAAMRWRKTLQGCSNKEPNVIVVQGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSL--FKETTHLFVDFS 1448 HSP 3 Score: 68.1662 bits (165), Expect = 8.322e-11 Identity = 52/201 (25.87%), Postives = 95/201 (47.26%), Query Frame = 2
Query: 2183 SNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPTVV-AGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
+ ++ GLF G FI + ++L +++ E +G+I SR + A + C E+ ++ T+ +++ C+ +L + LF P V G +P L CV+S+ F E D L + + LGA+VQD ++ + K + AST +V + ++ W +P V W+LE + AD ++L+
Sbjct: 532 AEASEGGLFFGKR-FILVGFGAEAEAQLSELVMENAGKILVG--RSRAVAHYAIVP-LLGCDVEATVDEVATDTWLAMCVEQQCVLPLASHPLFTPVAVREGFSP---LKDCVLSVSQFTGAERDSLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTIRWVLESARTGKRADEGQYLV 725
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Zebrafish
Match: ect2 (epithelial cell transforming 2 [Source:ZFIN;Acc:ZDB-GENE-040831-2]) HSP 1 Score: 70.4774 bits (171), Expect = 1.126e-11 Identity = 54/225 (24.00%), Postives = 98/225 (43.56%), Query Frame = 2
Query: 170 EKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVM-EKQKLINLIKMMEGTYSEKFTNS-IQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQ--IENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLK 832
E E + FV +F+ + +L + I GP +++C ++ LP + PL++ + N + + E L+NL+ M GT + F ++ + L+ R+ KY A +L+PDWI W + I NE + FK+P F + + GF E E+N G F + THL+ + + ++ + + +V W S++
Sbjct: 90 ENCEFETVFVLKDFQAPEYSFLYKNDSRILGPPAVMHCANKGEPLPFSSRPLYSMTMLNLSLCFTGFRKKDEVVALVNLVHHMGGTIRKDFDSAKVTHLIARSTHGEKYRLAVCMGTPILTPDWIRKAWEHRDDINFHAGNELFRTEFKVPPFQDCVLSFLGFSEEEKNN-MEERTQKHGGRFQAVGDERCTHLVVEENSIKELPFTPSKRLYVVKQEWFWGSIQ 313 HSP 2 Score: 55.0694 bits (131), Expect = 6.771e-7 Identity = 45/199 (22.61%), Postives = 87/199 (43.72%), Query Frame = 2
Query: 455 ILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFG-----ELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLH------DSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIID 1018
+ + ++ ++P+YS + N +L P + N + E PF+ + L + NL +C +GF R + + N H G + + THLI + + KY A+ I+ +W+ D + E + EF F+ C++SF+G S+E N + + + G+ + E+ TH +++
Sbjct: 97 VFVLKDFQAPEYSFLYKNDSRILGPPAVMHCAN-KGEPLPFSSRPLYSMTM---LNLSLCFTGF----RKKDEVVALVNLVHHMGGTIRKDFDSAKVTHLIARSTHGEKYRLAVCMGTPILTPDWIRKAWEHRDDINFHAGNELFRTEFKVPPFQDCVLSFLGFSEEEKNNMEERTQKHGGRFQAVGD--ERCTHLVVE 285
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Zebrafish
Match: ect2 (epithelial cell transforming 2 [Source:ZFIN;Acc:ZDB-GENE-040831-2]) HSP 1 Score: 70.0922 bits (170), Expect = 1.989e-11 Identity = 54/225 (24.00%), Postives = 98/225 (43.56%), Query Frame = 2
Query: 170 EKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVM-EKQKLINLIKMMEGTYSEKFTNS-IQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQ--IENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLK 832
E E + FV +F+ + +L + I GP +++C ++ LP + PL++ + N + + E L+NL+ M GT + F ++ + L+ R+ KY A +L+PDWI W + I NE + FK+P F + + GF E E+N G F + THL+ + + ++ + + +V W S++
Sbjct: 90 ENCEFETVFVLKDFQAPEYSFLYKNDSRILGPPAVMHCANKGEPLPFSSRPLYSMTMLNLSLCFTGFRKKDEVVALVNLVHHMGGTIRKDFDSAKVTHLIARSTHGEKYRLAVCMGTPILTPDWIRKAWEHRDDINFHAGNELFRTEFKVPPFQDCVLSFLGFSEEEKNN-MEERTQKHGGRFQAVGDERCTHLVVEENSIKELPFTPSKRLYVVKQEWFWGSIQ 313 HSP 2 Score: 55.4546 bits (132), Expect = 5.847e-7 Identity = 45/199 (22.61%), Postives = 87/199 (43.72%), Query Frame = 2
Query: 455 ILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFG-----ELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLH------DSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIID 1018
+ + ++ ++P+YS + N +L P + N + E PF+ + L + NL +C +GF R + + N H G + + THLI + + KY A+ I+ +W+ D + E + EF F+ C++SF+G S+E N + + + G+ + E+ TH +++
Sbjct: 97 VFVLKDFQAPEYSFLYKNDSRILGPPAVMHCAN-KGEPLPFSSRPLYSMTM---LNLSLCFTGF----RKKDEVVALVNLVHHMGGTIRKDFDSAKVTHLIARSTHGEKYRLAVCMGTPILTPDWIRKAWEHRDDINFHAGNELFRTEFKVPPFQDCVLSFLGFSEEEKNNMEERTQKHGGRFQAVGD--ERCTHLVVE 285
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314]) HSP 1 Score: 134.035 bits (336), Expect = 7.975e-31 Identity = 78/272 (28.68%), Postives = 140/272 (51.47%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFEDVKAVKL---SENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW---NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F KAY+++ + + ++++L SE ++ +K S ++ F G F +LK L I GP ++++C+ +P+ P++N + + IS + LD ++ + N +++M G S+ L+ V S KY A + +L P W+ +W N QI + + +K + PIF IC++G +R ++ + +GG + G+L NE+THLI + +K +KY A +WNV V + W DSL G+C++ Y+ P
Sbjct: 20 SEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDK----SLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIR--YCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEP 285 HSP 2 Score: 96.6709 bits (239), Expect = 2.369e-19 Identity = 58/178 (32.58%), Postives = 88/178 (49.44%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE----------FGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
R F L+ +E S +I +GG V+E D TH+I RN+K+L+++A+GKW++H SY+EAC +++ E YEWGS + + + + LA A RWR+ AF W++IL ++K FKR+ SGG V G S E H F + +
Sbjct: 1243 RRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRM-LAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVF-AGHSSALFKEANHLFADFS 1418 HSP 3 Score: 76.6406 bits (187), Expect = 2.905e-13 Identity = 102/425 (24.00%), Postives = 180/425 (42.35%), Query Frame = 2
Query: 1547 PSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEI--INNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGI-DLSI-DDQSPWISESTIQQETRIIDSNSTTDGLFNG----ILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQ 2785
P L D C I G +D + +I NSG VR FS + ++V + E L + +R + WL++S K L P EN + S + + + + IS + + + D+D + ++K+++E H ++++N I +S+ +D + S+S + + II+ GLF+ +L F+++ + ++ II + +G++ S R I A + C ES ++VT ++ CI +++LD + LF P + G P L CV+S+ F E D L + LGA VQ+ ++ K + AST +V KDA + ++ W++P V WLL+C + AD +L+ S Q
Sbjct: 348 PEDLLDGCRIYLCGFGGRKLDKLRRLI--NSGGGVR----FSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQP------------EN-IYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLSKNYIEDE-HPK---------ISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEG-----GLFSRKQFLVLGFLEED-----EACIIDIIKKNAGKVLS--SQKRAIADYAVVP-LLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRP---LQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSLQ 727
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314]) HSP 1 Score: 134.035 bits (336), Expect = 8.520e-31 Identity = 78/272 (28.68%), Postives = 140/272 (51.47%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFEDVKAVKL---SENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW---NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F KAY+++ + + ++++L SE ++ +K S ++ F G F +LK L I GP ++++C+ +P+ P++N + + IS + LD ++ + N +++M G S+ L+ V S KY A + +L P W+ +W N QI + + +K + PIF IC++G +R ++ + +GG + G+L NE+THLI + +K +KY A +WNV V + W DSL G+C++ Y+ P
Sbjct: 20 SEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDK----SLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIR--YCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEP 285 HSP 2 Score: 96.2857 bits (238), Expect = 2.789e-19 Identity = 58/178 (32.58%), Postives = 88/178 (49.44%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE----------FGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
R F L+ +E S +I +GG V+E D TH+I RN+K+L+++A+GKW++H SY+EAC +++ E YEWGS + + + + LA A RWR+ AF W++IL ++K FKR+ SGG V G S E H F + +
Sbjct: 1220 RRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRM-LAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVF-AGHSSALFKEANHLFADFS 1395 HSP 3 Score: 72.7886 bits (177), Expect = 4.433e-12 Identity = 55/207 (26.57%), Postives = 98/207 (47.34%), Query Frame = 2
Query: 2165 ETRIIDSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
++ + D+++ +G F+ + + ++ II + +G++ S R I A + C ES ++VT ++ CI +++LD + LF P + G P L CV+S+ F E D L + LGA VQ+ ++ K + AST +V KDA + ++ W++P V WLL+C + AD +L+
Sbjct: 532 QSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLS--SQKRAIADYAVVP-LLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRP---LQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLV 732
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314]) HSP 1 Score: 134.035 bits (336), Expect = 9.066e-31 Identity = 78/272 (28.68%), Postives = 140/272 (51.47%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFEDVKAVKL---SENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW---NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F KAY+++ + + ++++L SE ++ +K S ++ F G F +LK L I GP ++++C+ +P+ P++N + + IS + LD ++ + N +++M G S+ L+ V S KY A + +L P W+ +W N QI + + +K + PIF IC++G +R ++ + +GG + G+L NE+THLI + +K +KY A +WNV V + W DSL G+C++ Y+ P
Sbjct: 20 SEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDK----SLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIR--YCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEP 285 HSP 2 Score: 97.0561 bits (240), Expect = 1.893e-19 Identity = 58/178 (32.58%), Postives = 88/178 (49.44%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE----------FGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
R F L+ +E S +I +GG V+E D TH+I RN+K+L+++A+GKW++H SY+EAC +++ E YEWGS + + + + LA A RWR+ AF W++IL ++K FKR+ SGG V G S E H F + +
Sbjct: 1226 RRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRM-LAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVF-AGHSSALFKEANHLFADFS 1401 HSP 3 Score: 75.8702 bits (185), Expect = 4.419e-13 Identity = 103/423 (24.35%), Postives = 180/423 (42.55%), Query Frame = 2
Query: 1547 PSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEI--INNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGI-DLSI-DDQSPWISESTIQQETRIIDSNSTTDGLFNG----ILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVS 2779
P L D C I G +D + +I NSG VR FS + ++V + E L + +R + WL++S K L P EN + S + + + + IS + + + D+D + S++T N + +E H ++++N I +S+ +D + S+S + + II+ GLF+ +L F+++ + ++ II + +G++ S R I A + C ES ++VT ++ CI +++LD + LF P + G P L CV+S+ F E D L + LGA VQ+ ++ K + AST +V KDA + ++ W++P V WLL+C + AD +L+ S
Sbjct: 355 PEDLLDGCRIYLCGFGGRKLDKLRRLI--NSGGGVR----FSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQP------------EN-IYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADED----LLSQYTENNSTLIEDE-HPK---------ISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEG-----GLFSRKQFLVLGFLEED-----EACIIDIIKKNAGKVLS--SQKRAIADYAVVP-LLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRP---LQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDS 728
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314]) HSP 1 Score: 133.65 bits (335), Expect = 1.012e-30 Identity = 78/272 (28.68%), Postives = 140/272 (51.47%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFEDVKAVKL---SENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW---NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F KAY+++ + + ++++L SE ++ +K S ++ F G F +LK L I GP ++++C+ +P+ P++N + + IS + LD ++ + N +++M G S+ L+ V S KY A + +L P W+ +W N QI + + +K + PIF IC++G +R ++ + +GG + G+L NE+THLI + +K +KY A +WNV V + W DSL G+C++ Y+ P
Sbjct: 20 SEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDK----SLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIR--YCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEP 285 HSP 2 Score: 97.0561 bits (240), Expect = 1.817e-19 Identity = 58/178 (32.58%), Postives = 88/178 (49.44%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE----------FGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
R F L+ +E S +I +GG V+E D TH+I RN+K+L+++A+GKW++H SY+EAC +++ E YEWGS + + + + LA A RWR+ AF W++IL ++K FKR+ SGG V G S E H F + +
Sbjct: 1263 RRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRM-LAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVF-AGHSSALFKEANHLFADFS 1438 HSP 3 Score: 75.8702 bits (185), Expect = 4.563e-13 Identity = 103/423 (24.35%), Postives = 180/423 (42.55%), Query Frame = 2
Query: 1547 PSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEI--INNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGI-DLSI-DDQSPWISESTIQQETRIIDSNSTTDGLFNG----ILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVS 2779
P L D C I G +D + +I NSG VR FS + ++V + E L + +R + WL++S K L P EN + S + + + + IS + + + D+D + S++T N + +E H ++++N I +S+ +D + S+S + + II+ GLF+ +L F+++ + ++ II + +G++ S R I A + C ES ++VT ++ CI +++LD + LF P + G P L CV+S+ F E D L + LGA VQ+ ++ K + AST +V KDA + ++ W++P V WLL+C + AD +L+ S
Sbjct: 355 PEDLLDGCRIYLCGFGGRKLDKLRRLI--NSGGGVR----FSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQP------------EN-IYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADED----LLSQYTENNSTLIEDE-HPK---------ISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEG-----GLFSRKQFLVLGFLEED-----EACIIDIIKKNAGKVLS--SQKRAIADYAVVP-LLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRP---LQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDS 728
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314]) HSP 1 Score: 130.568 bits (327), Expect = 8.658e-30 Identity = 69/244 (28.28%), Postives = 124/244 (50.82%), Query Frame = 2
Query: 143 SENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
SE ++ +K S ++ F G F +LK L I GP ++++C+ +P+ P++N + + IS + LD ++ + N +++M G S+ L+ V S KY A + +L P W+ +W+ + + + +K + PIF IC++G +R ++ + +GG + G+L NE+THLI + +K +KY A +WNV V + W DSL G+C++ Y+ P
Sbjct: 11 SEALLLKEKDK----SLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEP 250 HSP 2 Score: 96.2857 bits (238), Expect = 2.548e-19 Identity = 58/178 (32.58%), Postives = 88/178 (49.44%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE----------FGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
R F L+ +E S +I +GG V+E D TH+I RN+K+L+++A+GKW++H SY+EAC +++ E YEWGS + + + + LA A RWR+ AF W++IL ++K FKR+ SGG V G S E H F + +
Sbjct: 1169 RRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRM-LAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVF-AGHSSALFKEANHLFADFS 1344 HSP 3 Score: 76.6406 bits (187), Expect = 2.816e-13 Identity = 104/431 (24.13%), Postives = 183/431 (42.46%), Query Frame = 2
Query: 1547 PSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEI--INNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGI-DLSI-DDQSPWISESTIQQETRIIDSNSTTDGLFNG----ILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQNYMISQ 2803
P L D C I G +D + +I NSG VR FS + ++V + E L + +R + WL++S K L P EN + S + + + + IS + + + D+D + S++T N + +E H ++++N I +S+ +D + S+S + + II+ GLF+ +L F+++ + ++ II + +G++ S R I A + C ES ++VT ++ CI +++LD + LF P + G P L CV+S+ F E D L + LGA VQ+ ++ K + AST +V KDA + ++ W++P V WLL+C + AD +L+ S +S+
Sbjct: 318 PEDLLDGCRIYLCGFGGRKLDKLRRLI--NSGGGVR----FSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQP------------EN-IYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADED----LLSQYTENNSTLIEDE-HPK---------ISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEG-----GLFSRKQFLVLGFLEED-----EACIIDIIKKNAGKVLS--SQKRAIADYAVVP-LLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRP---LQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSTYKPQVSR 699
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Mouse
Match: Topbp1 (topoisomerase (DNA) II binding protein 1 [Source:MGI Symbol;Acc:MGI:1920018]) HSP 1 Score: 134.035 bits (336), Expect = 6.250e-31 Identity = 80/274 (29.20%), Postives = 136/274 (49.64%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFE---------DVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYE 865
S F KA +S+ E + D +A+K+ EN S ++ F G F++LK L I GP ++ +C+ +P+ P++N + + +S + LD +++++ ++MM G S+ L+ V S KY A N +L P WI +W E + + ++ FK PIF IC++G R T++ + +GG + G+L NE THLI + K +KY A +WNV V + W HDS++ G+C++ + Y+
Sbjct: 20 SECFFKALESIKELQSEDYLQIITDEEALKIREN----------DKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYK 283 HSP 2 Score: 99.3673 bits (246), Expect = 2.533e-20 Identity = 62/177 (35.03%), Postives = 91/177 (51.41%), Query Frame = 2
Query: 3386 LFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAAND-RDDLLPLALCAKRWR-------EFG--GKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
+F ++ +E +I LGG V+E D TH++ RN+K+L+++A+GKW++H SY++AC R++ E YEWGSS + L A D + LAL A RWR E G AF W+ IL + FKR+ +GG VL G P + + TH F + N
Sbjct: 1265 VFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGSS--SILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVLS-GHPEPLLKDATHLFCDFN 1438 HSP 3 Score: 74.7146 bits (182), Expect = 8.044e-13 Identity = 60/202 (29.70%), Postives = 96/202 (47.52%), Query Frame = 2
Query: 2180 DSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPE---SKLVTEYYISQCIANDRILDVDGDFLFYP-TVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
DS +GLF+ F+ S + +V II E +G+I PS IV + C + ++VT ++ CI N ++D + LF P +V++G+ P L CVIS E D L + LGA+VQ+ ++ K + AST ++ K+ + ++ W +P VN WLLE + AD + FL+
Sbjct: 547 DSTVREEGLFSQ-KSFLVLGFSVENKCNIVDIIREHAGKIVSLPSR---IVADYAVVPLLGCEVDVTVGEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGVTP---LEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARIGKRADENHFLV 741
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Mouse
Match: Slf1 (SMC5-SMC6 complex localization factor 1 [Source:MGI Symbol;Acc:MGI:2145448]) HSP 1 Score: 66.6254 bits (161), Expect = 6.334e-12 Identity = 32/81 (39.51%), Postives = 49/81 (60.49%), Query Frame = 2
Query: 3395 LTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWG 3637
+TGF+ E L ++ +L ++S + THLI RL +++KFL+A A+GKW++ YI + RWL+ET YEWG
Sbjct: 12 MTGFKMEEKEALVKLLLKLDCTFIKSEKY-KNCTHLIAERLCKSEKFLAACAAGKWVLTKDYIIHSAKSGRWLDETTYEWG 91
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Mouse
Match: Slf1 (SMC5-SMC6 complex localization factor 1 [Source:MGI Symbol;Acc:MGI:2145448]) HSP 1 Score: 66.6254 bits (161), Expect = 6.334e-12 Identity = 32/81 (39.51%), Postives = 49/81 (60.49%), Query Frame = 2
Query: 3395 LTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWG 3637
+TGF+ E L ++ +L ++S + THLI RL +++KFL+A A+GKW++ YI + RWL+ET YEWG
Sbjct: 12 MTGFKMEEKEALVKLLLKLDCTFIKSEKY-KNCTHLIAERLCKSEKFLAACAAGKWVLTKDYIIHSAKSGRWLDETTYEWG 91
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Mouse
Match: Slf1 (SMC5-SMC6 complex localization factor 1 [Source:MGI Symbol;Acc:MGI:2145448]) HSP 1 Score: 67.781 bits (164), Expect = 1.270e-11 Identity = 38/120 (31.67%), Postives = 60/120 (50.00%), Query Frame = 2
Query: 3362 VSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE 3721
+ ++ + +TGF+ E L ++ +L ++S + THLI RL +++KFL+A A+GKW++ YI + RWL+ET YEWG D + KRWRE
Sbjct: 7 MEDSATKHIIQMTGFKMEEKEALVKLLLKLDCTFIKSEKY-KNCTHLIAERLCKSEKFLAACAAGKWVLTKDYIIHSAKSGRWLDETTYEWGYK------IEKDSHYSPQMQSAPKRWRE 119
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Mouse
Match: Slf1 (SMC5-SMC6 complex localization factor 1 [Source:MGI Symbol;Acc:MGI:2145448]) HSP 1 Score: 67.781 bits (164), Expect = 1.747e-11 Identity = 38/120 (31.67%), Postives = 60/120 (50.00%), Query Frame = 2
Query: 3362 VSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE 3721
+ ++ + +TGF+ E L ++ +L ++S + THLI RL +++KFL+A A+GKW++ YI + RWL+ET YEWG D + KRWRE
Sbjct: 1 MEDSATKHIIQMTGFKMEEKEALVKLLLKLDCTFIKSEKY-KNCTHLIAERLCKSEKFLAACAAGKWVLTKDYIIHSAKSGRWLDETTYEWGYK------IEKDSHYSPQMQSAPKRWRE 113
BLAST of DNA topoisomerase 2 binding protein 1 vs. UniProt/SwissProt
Match: sp|Q6ZQF0|TOPB1_MOUSE (DNA topoisomerase 2-binding protein 1 OS=Mus musculus OX=10090 GN=Topbp1 PE=1 SV=2) HSP 1 Score: 134.035 bits (336), Expect = 4.372e-30 Identity = 80/274 (29.20%), Postives = 136/274 (49.64%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFE---------DVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYE 865
S F KA +S+ E + D +A+K+ EN S ++ F G F++LK L I GP ++ +C+ +P+ P++N + + +S + LD +++++ ++MM G S+ L+ V S KY A N +L P WI +W E + + ++ FK PIF IC++G R T++ + +GG + G+L NE THLI + K +KY A +WNV V + W HDS++ G+C++ + Y+
Sbjct: 20 SECFFKALESIKELQSEDYLQIITDEEALKIREN----------DKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYK 283 HSP 2 Score: 99.3673 bits (246), Expect = 1.772e-19 Identity = 62/177 (35.03%), Postives = 91/177 (51.41%), Query Frame = 2
Query: 3386 LFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAAND-RDDLLPLALCAKRWR-------EFG--GKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
+F ++ +E +I LGG V+E D TH++ RN+K+L+++A+GKW++H SY++AC R++ E YEWGSS + L A D + LAL A RWR E G AF W+ IL + FKR+ +GG VL G P + + TH F + N
Sbjct: 1265 VFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGSS--SILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVLS-GHPEPLLKDATHLFCDFN 1438 HSP 3 Score: 74.7146 bits (182), Expect = 5.627e-12 Identity = 60/202 (29.70%), Postives = 96/202 (47.52%), Query Frame = 2
Query: 2180 DSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPE---SKLVTEYYISQCIANDRILDVDGDFLFYP-TVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
DS +GLF+ F+ S + +V II E +G+I PS IV + C + ++VT ++ CI N ++D + LF P +V++G+ P L CVIS E D L + LGA+VQ+ ++ K + AST ++ K+ + ++ W +P VN WLLE + AD + FL+
Sbjct: 547 DSTVREEGLFSQ-KSFLVLGFSVENKCNIVDIIREHAGKIVSLPSR---IVADYAVVPLLGCEVDVTVGEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGVTP---LEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARIGKRADENHFLV 741
BLAST of DNA topoisomerase 2 binding protein 1 vs. UniProt/SwissProt
Match: sp|Q800K6|TOB1A_XENLA (DNA topoisomerase 2-binding protein 1-A OS=Xenopus laevis OX=8355 GN=topbp1-A PE=1 SV=2) HSP 1 Score: 130.568 bits (327), Expect = 5.201e-29 Identity = 73/272 (26.84%), Postives = 143/272 (52.57%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFEDVKAVKLSEN---FIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW---NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F KAY+++ + + ++++L+E ++ +K S ++ F G AF +LK L I GP ++++C+ +P+ P++N + + IS + LD ++ + + +++M G S+ L+ V S KY A + +L P W+ ++W N +I +++ + + PIF IC++G +R ++ + +GG + G+L NE+THLI + +K +KY A +WNV + + W DS++ G+C++ Y+ P
Sbjct: 20 SEYFFKAYEAIKQIQSDESLQLTEEREALLLKEKDK----SLYICDPFSGAAFSHLKKLGCRIVGPQVVIFCMENQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHHYVQIMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKELWEKSNQRIIR--YSDVNMTEYLCPIFRGCTICVTGLSSLDRKEVQRLTALHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCISVQWFFDSIEKGFCQDETMYKIEP 285 HSP 2 Score: 103.219 bits (256), Expect = 1.329e-20 Identity = 60/178 (33.71%), Postives = 90/178 (50.56%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE----------FGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
R F L+ +E S +I LGG V+E D TH++ RN+K+L+++A+GKW++H SY+EAC R++ E YEWGS + + + + LA A RWR+ AF W++IL ++K FKR+ SGG V G S P E +H F + +
Sbjct: 1261 RRFQLSSLDPQERIDYSQLIEELGGVVIEKQCFDPSCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKRFIQEEDYEWGSMSILSAVTNINPQQRM-LAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVF-AGHSSPLFKEASHLFADFS 1436 HSP 3 Score: 75.0998 bits (183), Expect = 4.913e-12 Identity = 104/427 (24.36%), Postives = 180/427 (42.15%), Query Frame = 2
Query: 1547 PSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEIIN---NRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGC--SDGFIGKWTAIINSRSGRVISESEFSITPPTHI---VVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGI-DLSI-DDQSPWISESTIQQETRIIDSNSTTDGLFNG----ILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
P L D C I G +D + +I N G VR F+ + ++V + E L + +N +R + WL+ S K L P + I FH + IN + ++ S + P H D+D + S++T N + +E H +++N I +S+ +D + S+S + + II+ GLF+ +L F+++ + ++ II + +G++ S R I A + C ES ++VT ++ CI +++LD + LF P + G P L CV+S+ F E D L + LGA VQ+ ++ K + AST +V KDA + ++ W++P V +WLL+C + AD +L+
Sbjct: 355 PDDLLDGCRIYLCGFGGRKLDKLRKLINNGGG--VR----FNQLTGDVTHIIVGETDEELK-QFLNKTQHRPYVLTVKWLLDSFAKGHLQPEE--------------IYFHSSYQQTEMPSPFEPAINLTANKMSSTRD----PLNHTRNHQADED----LLSQYTENNSTLIEDE-HPK---------TSNTNSISQMSMHEDMTTCTSQSGLADTSTIIEG-----GLFSRKQFMVLGFLEED-----EACIIDIIKKSAGKVLS--SQKRAIADYAVVP-LLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPFLEGSTP---LRECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKGMFASTHLVLKDAEGSKYEAAKKWNLPAVTMNWLLQCARTGRKADEDSYLV 726
BLAST of DNA topoisomerase 2 binding protein 1 vs. UniProt/SwissProt
Match: sp|Q92547|TOPB1_HUMAN (DNA topoisomerase 2-binding protein 1 OS=Homo sapiens OX=9606 GN=TOPBP1 PE=1 SV=3) HSP 1 Score: 130.568 bits (327), Expect = 5.220e-29 Identity = 79/277 (28.52%), Postives = 137/277 (49.46%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFE---------DVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F KA +S+ EF+ + +A+K+ EN S ++ F G F++LK L I GP ++++C+ +P+ P++N + + IS + L+ +++++ ++MM G S+ L+ V S KY A N +L P WI +W E + + ++ FK PIF IC++G +R ++ + +GG + G+L NE THLI + K +KY A +WNV V W DS++ G+C++ + Y+ P
Sbjct: 20 SKCFFKALESIKEFQSEEYLQIITEEEALKIKEN----------DRSLYICDPFSGVVFDHLKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTDINMEDFKCPIFLGCIICVTGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDESIYKTEP 286 HSP 2 Score: 113.235 bits (282), Expect = 1.042e-23 Identity = 69/177 (38.98%), Postives = 96/177 (54.24%), Query Frame = 2
Query: 3386 LFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGT-NTLIAANDRDDLLPLALCAKRWR-------EFG--GKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
+F L+ +E +I +LGG V+E D TH++ RN+K+L++VA+GKW++H SY+EAC ++ E YEWGSS + L N + LAL A RWR E G AF W++IL +S+ FKR+ SGG VLP G S+P E TH F +LN
Sbjct: 1269 IFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGSSSILDVLTGINVQQRR--LALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLP-GHSVPLFKEATHLFSDLN 1442 HSP 3 Score: 67.0106 bits (162), Expect = 1.183e-9 Identity = 58/201 (28.86%), Postives = 93/201 (46.27%), Query Frame = 2
Query: 2183 SNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
S T +GLF+ F+ S+ + S + II E +G+I S + V + C E+ ++VT ++ CI + D + LF P V+ G+ P L CVIS E + L + LGA+VQ+ ++ K + AST ++ K+ + ++ W++P V WLLE + AD S FLI
Sbjct: 545 STITEEGLFSQ-KSFLVLGFSNENESNIANIIKENAGKIMSLLSRT---VADYAVVPLLGCEVEATVGEVVTNTWLVTCIDYQTLFDPKSNPLFTPVPVMTGMTP---LEDCVISFSQCAGAEKESLTFLANLLGASVQEYFVRKSNAKKGMFASTHLILKERGGSKYEAAKKWNLPAVTIAWLLETARTGKRADESHFLI 738
BLAST of DNA topoisomerase 2 binding protein 1 vs. UniProt/SwissProt
Match: sp|Q7ZZY3|TOB1B_XENLA (DNA topoisomerase 2-binding protein 1-B OS=Xenopus laevis OX=8355 GN=topbp1-B PE=2 SV=1) HSP 1 Score: 125.176 bits (313), Expect = 2.283e-27 Identity = 73/273 (26.74%), Postives = 141/273 (51.65%), Query Frame = 2
Query: 77 SPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKL---ESNSC-FVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW---NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
S F K Y+++ + + ++++L+E E L E + C ++ F G AF +LK L I GP ++++C+ +P+ P++N + + IS + LD ++ + + +++M G S+ L+ V S KY A + +L P W+ ++W N +I +++ + + PIF IC++G +R ++ + +GG + G+L NE+THLI + +K +KY A +W V + + W DS++ G+C++ Y+ P
Sbjct: 20 SEYFLKGYEAMKQIQSDESLQLTEE-----REALVLKEKDKCVYICDPFSGAAFSHLKKLGCRIVGPQVVIFCMESQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHHYVQIMGGCVYRDLNVSVTHLIAGEVGSNKYLVAASLEKPILLPSWVKELWEKSNQRIIR--YSDVNMTEYLCPIFRGCTICVTGLSSLDRKEVQRLTALHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWIVHCISVQWFFDSIEKGFCQDETMYKIEP 285 HSP 2 Score: 103.219 bits (256), Expect = 1.296e-20 Identity = 60/178 (33.71%), Postives = 90/178 (50.56%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWRE----------FGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
R F L+ +E S +I LGG V+E D TH++ RN+K+L+++A+GKW++H SY+EAC R++ E YEWGS + + + + LA A RWR+ AF W++IL ++K FKR+ SGG V G S P E +H F + +
Sbjct: 1261 RRFQLSSLDPQERIDYSQLIEELGGVVIEKQCFDPSCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKRFIQEEDYEWGSMSILSAVTNINPQQRM-LAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVF-AGHSSPLFKEASHLFADFS 1436 HSP 3 Score: 66.6254 bits (161), Expect = 1.699e-9 Identity = 39/133 (29.32%), Postives = 67/133 (50.38%), Query Frame = 2
Query: 2378 KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
++VT ++ CI +++LD + LF P + G P L CV+S+ F E D L + LGA VQ+ ++ K + A + + KDA + ++ W++P V +WLL+C + AD +L+
Sbjct: 597 EVVTNAWLGMCIEQEKLLDPHSNALFTPVPFLEGSTP---LRECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKGMFARSHLDLKDAEGSKYEAAKKWNLPAVTMNWLLQCARTGKKADEDSYLV 726
BLAST of DNA topoisomerase 2 binding protein 1 vs. UniProt/SwissProt
Match: sp|P32372|RAD4_SCHPO (S-M checkpoint control protein rad4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rad4 PE=1 SV=2) HSP 1 Score: 83.9593 bits (206), Expect = 4.724e-15 Identity = 51/185 (27.57%), Postives = 98/185 (52.97%), Query Frame = 2
Query: 329 NRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNL--ITVLSPDWIYDIWNSQIENDPFNEQYL--KRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFN 871
++ LK + I + +D+ ++ ++ + Y FT + L+ + ++PKY A + I ++S +WI ++ S ++ + ++ L K F +P F ++CL+ +PER+ I+N + +GG +LT + THLI S RKY YAL+W + +VC+ WL S++ E ++ +
Sbjct: 4 SKPLKGFVICCTSIDLKQRTEISTKATKLGAAYRSDFTKDVTHLIAGDFDTPKYKFAAKSRPDIKIMSSEWIPVLYESWVQGEDLDDGLLVDKHF-LPTLFKCRVCLTNIGQPERSRIENYVLKHGGTFCPDLT-RDVTHLIAGTSSGRKYEYALKWKINVVCVEWLWQSIQRNAVLEPQYFQLD 186
BLAST of DNA topoisomerase 2 binding protein 1 vs. TrEMBL
Match: A0A0R3WND6 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS2261 PE=4 SV=1) HSP 1 Score: 175.252 bits (443), Expect = 1.332e-40 Identity = 134/472 (28.39%), Postives = 227/472 (48.09%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKL-SENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNE---QYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNIFEHCII-SFIGCSDEFINQWKVIIENNNGKVS--LDPNLIEKTTHFIIDNKVGLITKNYGNYVDKSWLEKSINNGKRLPLKEFLVLGEIPNEVSSDFYSPICGDIFSNCLIMFHGFSNEFIDKWTKXXXXXXXXXXXXKQPSQQQNPTHVVVRSSENLPIKS-----QVSYVTENWLEQSIFHRKQMPVRDFQFPL 1444
D+ +P+ + A+D++ +E+++ V + E+ I + +N CFV +F G +E+ + L ++FGP ++L + ++ LP PLF+ L+N ++ + + ++++L + I+M+ G S T+ + +++ V S KY + + +L+P+WI + W DP + L RFK+PIF L IC+SG ER + +I+ NNGG++ GE+ THL+ K + KY++A +W V++V + WL DS++ GY E Y EH S S +++ V+ G +S DPN+ T F S L+ N G P++ S + + C+I +G +K KIIE+S G + + THVV ++ PI S +V YVT +WLEQS K++P +DFQ PL
Sbjct: 12 RDAVTPLQNLAFDAVTSYENLRPVWIDGEDAIERFKDSSPNNCCFVLGKFVGTVYEHFRDLRSSVFGPQVLLNYLREERPLPNVSHPLFSTALRNANVTVTSITGQQRERLFSSIEMLHGVASRNLTDEVNVVIAPKVGSKKYVVGASRGLHILTPEWIDEAWKLSETVDPIDMLQCSLLSRFKLPIFAQLVICVSGLTPEERREVADIVKNNGGVYSGEMKIGLTTHLLVKRAGGLKYNFAKKWKVRVVSVRWLSDSIRKGYALEETDYYIREGKEEHPKCHSSTPTSSIYVSDPSVL-----GDLSSIADPNVELDETRF-------------------SCLKDLTN-------------GAFPSKSSKKSF-------LTGCIIFLYGCDPSESEKLAKIIEISGGILCIDASEKVTGSVTHVVFGTNSK-PISSSDLETEVFYVTRDWLEQSFEQNKRLPEKDFQPPL 438 HSP 2 Score: 73.559 bits (179), Expect = 3.345e-9 Identity = 81/366 (22.13%), Postives = 145/366 (39.62%), Query Frame = 2
Query: 1832 ESNMFENCLISFHGCSDGFIGKWTAIINSRSGRV-ISESEFSITPPTHIVVDDDW----ALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFL------------------------------------------VTDSNGIDLSIDDQS----------PWISESTIQQETRIIDSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRIL--DVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLA 2752
+ + C+I +GC K II G + I SE TH+V + + +++ YV + WLEQS K LP F + D +D +++DQ+ + +S I + +I ++ GLF+ ++ +D + S + L + IT++ G + ++S ++ ++ + S ++T Y+I+ C+ +L ++D F P + + L L GCVISL GF + L + LGA VQ+ ++ +K L A+T +VA + + W +P V+ WL C T + A
Sbjct: 345 KKSFLTGCIIFLYGCDPSESEKLAKIIEISGGILCIDASEKVTGSVTHVVFGTNSKPISSSDLETEVFYVTRDWLEQSFEQNKRLPEKDFQPPLRDKSESSDQSSDDKHSGVPVDTEEIQLINQYFKNDELCDMDDFRSVDNNLEDQAFQADAEETKLVTLKQSEIHGQQEVIPPDA---GLFSHLIFKLDINFSLTAHPFLSEAITDEGGSVV---NDSSVVADYIICSYILRQPTTSNMITPYWINCCVTAKTLLISELDTQVGFRPIHIPA-SVTLPLAGCVISLSGFAGNDRAFLTDLARGLGAVVQECFLRKAVPSKDLIANTHLVAARPDGRKWPASKQWGLPAVSCSWLYACATTGRRA 703
BLAST of DNA topoisomerase 2 binding protein 1 vs. TrEMBL
Match: A0A504YNB2 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_11559 PE=4 SV=1) HSP 1 Score: 169.088 bits (427), Expect = 2.371e-38 Identity = 88/276 (31.88%), Postives = 150/276 (54.35%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLES-NSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFN---EQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNI 880
ND + + A+D++ +E++ A +L +M Y S +CFVF F+GE FE+L+SL I+GP IIL+ + ++ LP+ PL++ L + + + E++ L++ I+M+ G S + I +++T V S KY + + +L P WI + W DP + + +RF++PIF L IC+SG ER + +++ +GG + G + E THLI K KY++A +W ++IV +NWL DS+ GY + Y + +I
Sbjct: 12 NDYATALLTAAFDAVSAYENLSAKRLDAAEVMMRYSTRPSKRTCFVFCRFEGECFEHLRSLGAAIYGPQIILHYIRENQPLPRVHHPLYSTALMRAVATVTSVTGSEREYLLSSIQMLHGQASRDLFDGINVVITPKVGSKKYLVGSSRGVNMLLPSWITEAWKLSAIQDPIDMMLPTFTERFRVPIFAQLVICVSGLSAEERKEVSDLVVKHGGRYSGMMKVGETTHLIIKHPSGLKYAHAKKWKIQIVSVNWLTDSVSKGYALDEVDYRVSESI 287 HSP 2 Score: 140.584 bits (353), Expect = 1.440e-29 Identity = 80/210 (38.10%), Postives = 113/210 (53.81%), Query Frame = 2
Query: 3395 LTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLI-----AANDR-------------DDLLPLALCAKRWREFGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINL--NCTNYPKLQLPFEYHWIKINFILSYLV 3964
+G E + + I+ LGGEV L THLI + R++K L ++SG+W++H SY++AC RESRW++ET YEWG GT L+ A + R + LA A+RWR GG+AFK WR+I K SF+R+ SGGG +L PP VTHSF N +N P LP Y W++I+++ +YL+
Sbjct: 1275 FSGLATDERDQYAAIVADLGGEVDNQLTLSEKATHLIVHTPTRSEKCLICLSSGRWMLHKSYLDACVRESRWVDETPYEWGGPGTEPLLMQLSPAVSTRVGHQQQQQQKQQAAQIRELARSARRWRMVGGEAFKGWRVIFGPGCDKEASFRRVIESGGGQILSSQPPYPPPTSVTHSFAKRRPNNSNAP---LPRGYQWLRIDYLCAYLI 1481 HSP 3 Score: 113.235 bits (282), Expect = 2.884e-21 Identity = 109/415 (26.27%), Postives = 173/415 (41.69%), Query Frame = 2
Query: 1832 ESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPP-THIVVDDDWA----LSIDSRFTYVNKSWL----------EQSIHAGKCL-PVDIFLVTDSNGIDL-----------------------SIDDQSPWISESTIQQETRIIDSNST-----------------------TDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRIL--DVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFL-----IVSNQNYMISQSAKKSELETPQSESVKVETQIN 2869
E + +CLI C+ K+ +++ GR+ ++ + +T P TH VV A +++ YV SWL +S H K + PV+ T ++G+D + D + E+T+QQ +++ S+ G F+G F+ + I +++++ + G+ITE + +VT F+ K +K+VT +I CIA I D+ G+ F P V +G P L GCVISL GF Y+ L RYS LGA VQD + +++L AST +VA + W +PVVNR+WL C + +LAD + I+S N+ +KS P + E Q N
Sbjct: 343 ERPILSDCLIQLIECNREEHFKYASLVKELGGRLCTDLQDRVTEPLTHAVVGSLNASGNVTTMEKEVAYVRGSWLISCHETHRRVPESEHLVKSVEPVEAEEPTMTDGLDSDDKTKRTMDTEDLQLINQYFGAGGLADVDDFNRENTVQQNASKVETRSSHGVPETHTLCEPHASGLNSTVAVRTGCFSGFSFFLHEEIPASDKLGVLEVVRDAGGQITEAIHAANFLVTPFFIRSNPKMSQNTKMVTVSWIDHCIAASTICWDDLIGEKAFMPLVRSGPLP---LDGCVISLSGFVGYDRVLLTRYSRALGAIVQDYFLCKAVPSRNLAASTHLVAAKLEGRKCPAAKQWGLPVVNRNWLYACADQWKLADTGAYPVEDPSIISKPNF-----TEKSAPSDPPKDRRLTEIQQN 749
BLAST of DNA topoisomerase 2 binding protein 1 vs. TrEMBL
Match: A0A183KJ35 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS15042 PE=4 SV=1) HSP 1 Score: 166.777 bits (421), Expect = 3.977e-38 Identity = 104/416 (25.00%), Postives = 198/416 (47.60%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYE-KLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQ---YLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFN----------PN-------------------------------------------IFEHCIISFIGCSDEFINQWKVIIENNNGKVSLD--PNLIEKTTHFIIDNKV---GLITKNYGNYVDKSWLEKSINNGKRLPLKEFLV 1126
N+ PS + + A++++ E+E++ +LS + ++ Y K + CFV + F GE +++L+SL +I+GP +IL+ V ++ LP PLF+ L N + + + +++++ + I+M+ G+ S T+++ +++T V S KY + + +L PDWI + W DP + Y +++++PIF L IC+SG ER + ++++ +GG + G + E THL+T+ + KY +A +W ++I+ I WL DS+ GY + Y + PN I C I F C + ++ + I+ GK+ + + E TH ++ + G+ + YV +WL ++ R+P E+L+
Sbjct: 8 NEPPSSLLNAAFNAVSEYENICPKRLSSSEVIKLYPLKPSRDVCFVLARFDGECYDHLRSLGVSIYGPQVILHYVRENKPLPNLPYPLFSTALLNATATVTSVTGAQRKQIFDSIQMLHGSVSRDLTDNVNVIVTAKVGSKKYLVGASRNLHILLPDWITEAWRLSEIQDPLDMMLGIYTEKYRVPIFSQLVICVSGLSVEERKEVSDLVSKHGGKYSGVMKIGETTHLVTRQASGTKYVHAKKWKIQIISIKWLIDSVDKGYALDEEDYRVDQVKTKSSTPTPNTQQNEISFDNISAISYVGAASKVDETRSVCFRESAQVVQSGSSILSGCSIRFSDCKKDEVHLYSSIVRELGGKLCTELFDQVTEMLTHVVVGSTPSSNGMSREKGVKYVKGAWLLACRDSKVRIPEDEYLI 423 HSP 2 Score: 78.9518 bits (193), Expect = 6.832e-11 Identity = 83/367 (22.62%), Postives = 143/367 (38.96%), Query Frame = 2
Query: 1835 SNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPP-THIVVDDDWA---LSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTD---------------SNGIDLS-------------IDDQSPWISESTIQQETRIIDSNSTT--------------------DGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRIL--DVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
S++ C I F C + +++I+ G++ +E +T TH+VV + +S + YV +WL + +P D +L+ + S ID + DD+ + I+ + I D TT G F+ ++ + +SD + II G I++N N +V F+ + VT +IS CI + ++ ++ F P + P L GCVISL GF + L Y+ LGA VQ+ ++ +++L AST ++A + + W +P VNR WL +C L + F +
Sbjct: 334 SSILSGCSIRFSDCKKDEVHLYSSIVRELGGKLCTELFDQVTEMLTHVVVGSTPSSNGMSREKGVKYVKGAWLLACRDSKVRIPEDEYLIPEINENLQRLEDEVDVTSTNIDAADFQLINQYFTGGLADDE---FQDEAIETKEAIQDFAKTTIRPEIADEVKHPEVSEISYPGGCFSNLIFSFHKELSDADVVKYTDIIKTAGGCISDNERNVNFLVAPFFVISLPPIS-SCEFVTIGWISYCIHEKSLCLQEIRKEYAFKPVLHKPGPPPL--SGCVISLSGFVGNDRSLLTSYAQALGAVVQECFLRKSVASRNLAASTHLIAAKPDGRKWPAAQQWGLPAVNRLWLYKCAETWSLVNECEFPV 694
BLAST of DNA topoisomerase 2 binding protein 1 vs. TrEMBL
Match: H2KQP9 (DNA topoisomerase 2-binding protein 1 OS=Clonorchis sinensis OX=79923 GN=TOPBP1 PE=4 SV=1) HSP 1 Score: 167.933 bits (424), Expect = 5.482e-38 Identity = 97/314 (30.89%), Postives = 160/314 (50.96%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEK-LESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFN---EQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDE-----FINQWKVIIENNNGKVSLD 979
ND +P+ A+D++ +E++ +L E IM+ Y K L + FVF F+GE FE+L+S+ I+GP IIL+ V ++ +LPK PLF+ L + + + E++ L+ I+M+ G S + + +++T V S KY + + +L P WI + W DP + Y +R+++P+F L IC+SG ER + +++ +GG + G + E THLI K + KYS+A +W ++ V + WL DS+ GY + Y + E C S D + VI NN +D
Sbjct: 51 NDHATPLLSAAFDAVSSYENLTTKRLDEVEIMNRYPKQLPKRTSFVFCRFEGECFEHLRSMGAAIYGPQIILHYVRENKALPKVSYPLFSTALMGAIATVTSVTGSEREYLLRSIQMLHGQASRDLFDGVNVVITPKVGSKKYLVGASRGLHILLPSWISEAWRLSSIQDPIDMMLPAYTERYRVPVFSQLVICVSGLSAEERKEVSDLVLKHGGRYSGVMKVGETTHLIIKQAVGLKYSHAKKWKIQTVSLRWLVDSVNKGYALDEEDY----RVSESCETSSTPVGDANKRSFSFDNISVISHVNNASTRID 360 HSP 2 Score: 144.436 bits (363), Expect = 9.451e-31 Identity = 79/219 (36.07%), Postives = 123/219 (56.16%), Query Frame = 2
Query: 3380 PRLFLLT--GFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLI-------------AANDR--------DDLLPLALCAKRWREFGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFI-NLNCTNYPKLQLPFEYHWIKINFILSYLV 3964
PR ++++ G + + ++ +LGGEV + THLI + R++K+L +A+GKWI+H SY++AC RESRW++E +EWG GT L+ A N + + + LA A+ WR GGKAFK+WR+I K SF+R+ +G G VL G PP + VTHSF + + T+ + QLP +Y W++I+++L+YL
Sbjct: 1217 PRRYVISFSGLPNDARDHYAHVVSQLGGEVDSQLTISEHTTHLIVHTPTRSEKYLICLATGKWILHKSYLDACLRESRWVDECNFEWGGPGTEPLLMQLSPAVLAVGPNANNSKVHEQRQQANQMRELARAARHWRLAGGKAFKNWRVIFGPGCDKETSFRRVIEAGAGQVLASGPPYPPASSVTHSFYKSRSSTSVNRNQLPADYEWLRIDYLLAYLT 1435 HSP 3 Score: 85.1149 bits (209), Expect = 1.036e-12 Identity = 59/197 (29.95%), Postives = 89/197 (45.18%), Query Frame = 2
Query: 2168 TRIIDSNSTT----DGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIAND--RILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTR 2740
T I +N +T DG F+G+ + +S L K I G + ++P N +V F++ K LVT +I CIA ++ D+ + F P G P L CVISL GF + L Y+ LGA VQ+ ++ +++L AST +V+ + + W IP V+R WL C R
Sbjct: 542 TDITVANGSTTVNGDGFFSGLSFLLHDELSAKIREELRKSICSSGGRVVDSPHNVDFLVAPFFVSSLPKLPKSCMLVTAPWIEHCIAESGLQVDDLLKEPAFVPIERTGAPP---LEECVISLSGFVGRDRSLLTAYAQCLGALVQECFLRKPVPSRNLAASTHLVSAKPDGRKWPAAQSWGIPTVSRSWLYACAER 735
BLAST of DNA topoisomerase 2 binding protein 1 vs. TrEMBL
Match: A0A183AXV7 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS11792 PE=4 SV=1) HSP 1 Score: 166.392 bits (420), Expect = 5.561e-38 Identity = 85/271 (31.37%), Postives = 148/271 (54.61%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNY-EKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFN---EQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYE 865
ND + + A+D++ +E++ A +L +++ Y K +CFVF F+GE FE+L+SL I+GP IIL+ + ++ LP+ PL++ L + + + E++ L++ I+M+ G S + + +++T V S KY + + +L P WI + W DP + ++ RF++PIF L IC+SG ER + +++ +GG + G + E THLI K KY++A +W ++IV +NWL DS+ GY + Y
Sbjct: 12 NDYATALLTAAFDAVSAYENLSAKRLDAAEVLNRYPTKPSKRTCFVFCRFEGECFEHLRSLGAAIYGPQIILHYIRENQPLPRVSHPLYSTALMRAVATVTSVTGSEREYLLSSIQMLHGQASRDLFDGVNVVITPKVGSKKYLVGSSRGVNMLLPSWITEAWKLSAIQDPIDMMLPTFMDRFRVPIFAQLVICVSGLSAEERKEVSDLVVKHGGRYSGMMKIGETTHLIIKHPSGLKYAHAKKWKIQIVSVNWLTDSVSKGYALDEMDYR 282 HSP 2 Score: 100.523 bits (249), Expect = 1.548e-17 Identity = 95/379 (25.07%), Postives = 158/379 (41.69%), Query Frame = 2
Query: 1829 IESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPP-THIVVDDDW-----ALSIDSRFTYVNKSWL--------------------------EQSIHAGKCLPVDIFLVTDSNGIDL--------SIDDQSPWISESTIQQ------------ETR------IIDSNSTTD---GLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRIL--DVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIV 2776
+E + +CLI C+ K+++++ GR+ ++ + +T P TH VV A +++ YV SWL EQ++ A L D+ + L + D + E+T++Q ETR + D ++T G F+G + ++ + +++ I + G+IT++ + IV F+ ++ E LVT +I CIA I D+ + F P V + P L GCVISL GF + L RYS LGA VQD + +++L AST +VA + W +PVVNR+WL C + +L D + + +
Sbjct: 342 VERPILADCLIQLIECTREEHTKFSSLVKELGGRLCTDLQDRVTEPLTHAVVGSSNSTKLNAATMEKDVFYVKGSWLIACQEAHKRVSETEHLANSPESDETEEQTLAADGELDDRSKQTMDTEDLQLINQYFGAGGLADMDDFNRENTVRQQNVTKAGTQVASETRQLCEALVSDMDATVQVRTGCFSGYVFYLHDQVPSSDRMEVIESIRDAGGQITDSSLVASFIVAPFFIQNKPPSSAECTLVTVNWIGHCIAASSICWDDLKCEKAFTPLVRSASLP---LDGCVISLSGFVGRDRTLLTRYSQALGAVVQDYFLCKAIPSRNLAASTHLVAAKLEGRKCPAAKQWGLPVVNRNWLYACADQWKLVDTAAYPVT 717
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Cavefish
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:NCBI gene;Acc:103036620]) HSP 1 Score: 135.961 bits (341), Expect = 1.406e-31 Identity = 78/261 (29.89%), Postives = 141/261 (54.02%), Query Frame = 2
Query: 92 KAYDSLLEFEDVKAVK-LSENFIMSNYEKLESN--SCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW-NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKY 862
KAY++++E + K V+ + E + KL+ N S +VFS+F AFE+ + L I P ++L+C+ Q +PK P++N + + ++ + LD + +++L+++M G S+ L+ V S KY A N VL P W+ W SQ +++ ++ + P+F +C++G ER ++ + +GG + G+L NE THLI +KY +A +WNV + ++WL DS++ G+C+E ++Y
Sbjct: 26 KAYEAIVELQSEKYVQTIGEEAAL----KLDHNDKSLYVFSDFTSSAFEHCRKLGCRIVSPLVVLFCLQQQRCVPKAEQPVYNMAMADVTVTCTSLDKEVRTDVMDLVQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAANLGKPVLLPTWVKACWEKSQDSMFRYSDLRVEDYLCPVFKGCTVCVTGLSTVERKEVQRLCELHGGTYTGQLKMNECTHLIVSEPSGQKYEFARKWNVYCISVHWLFDSIEKGFCQEESRY 282 HSP 2 Score: 103.605 bits (257), Expect = 1.051e-21 Identity = 66/179 (36.87%), Postives = 92/179 (51.40%), Query Frame = 2
Query: 3380 PRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGS----SGTNTLIAANDRDDLLPLALCAKRWRE-FGGK-----AFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
P F L+ +E S +I LGG VLE D +H+I RN+K+L+A+A+GKWI+H SY+EAC E +L E YEWGS T+ + R LAL A RWR+ G+ AF W ++L ++ F+R+ SGG VLP G S + TH F + +
Sbjct: 1275 PPRFQLSSLSPQERIDYSHVIEELGGIVLEKQCFDPSCSHIIVGYPLRNEKYLAAMAAGKWILHRSYLEACRAEGYFLVEEDYEWGSRSILDAVPTVTSQQKR-----LALSALRWRKALQGRADKEGAFGGWIVMLNIDHARDAGFRRLLQSGGAKVLP-GPSPSLYKDTTHLFADFS 1447 HSP 3 Score: 62.003 bits (149), Expect = 4.651e-9 Identity = 37/133 (27.82%), Postives = 66/133 (49.62%), Query Frame = 2
Query: 2378 KLVTEYYISQCIANDRILDVDGDFLFYPTVVA-GINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
++ T+ +++ C+ +L + LF P V G P L CV+S+ F E + L + + LGA+VQD ++ + K + AST +V + + W +P V W+LE ++A+ SR+L+
Sbjct: 598 EVATDSWLAMCVEQQCLLPLGSHPLFTPVAVKEGCLP---LKECVLSVSQFTGAERESLIQLAKHLGASVQDYFVRTANQRKGMLASTHLVLQTPEGTKYVAAQKWGLPAVTMRWILESARTGKVANESRYLV 727
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Cavefish
Match: ect2 (epithelial cell transforming 2 [Source:ZFIN;Acc:ZDB-GENE-040831-2]) HSP 1 Score: 75.0998 bits (183), Expect = 3.685e-13 Identity = 55/226 (24.34%), Postives = 102/226 (45.13%), Query Frame = 2
Query: 170 EKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKM---MEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW--NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLK 832
E E + FV +F+ + YL + I GP +IL+C + LP + PL++ + N + + +K++++NL+ + M GT + F+ + L+ + KY A +L+PDWI W +I E++ FK+P F + + GF E E+N G F E+ + THL+ + + ++ + + +V W S++
Sbjct: 59 ENAEFETVFVLKDFQSPYYSYLYKNDNRILGPPVILHCAGKGEPLPFSSRPLYSTTMLNMSLCFTGF--RKKEEVVNLVSLVHHMGGTIRKDFSAKVTHLIAHSTHGEKYRLAVCMGTPILTPDWIRKAWEHKDEINFHAGVEEFRTAFKVPPFQDCVLSFLGFSEEEKNN-MEERTLKHGGQFLEVGDEKCTHLVVEENSIKELPFVPSKRLYVVKQEWFWGSIQ 281
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Sea Lamprey
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-060626-1]) HSP 1 Score: 120.553 bits (301), Expect = 1.606e-27 Identity = 75/273 (27.47%), Postives = 140/273 (51.28%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAV-KLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNP 874
N++ S F +AY+ + + + + ++ +++ EK S +V F G AF++L + + GP ++L+C+ ++ +PK P++N L + +S + LD ++++ LI +M G FT+++ L+ V S KY A + ++ WI W + E + ++L++ P + ++G ER I+ ++ +GG + GEL NE THLI +KY +A +WNV V + WLHDS++ G C++ + Y P
Sbjct: 17 NENESKYFKEAYEKICKIQSESLLQRVDAAKVLTLKEK--DRSLYVCDPFHGVAFDHLVKMGCRVVGPQVVLFCLDRERCVPKAEHPVYNMALADAVLSCTNLDKDVREEMHRLIGLMGGRVLRDFTSAVTHLVAGEVGSKKYLVAASLKKPIMLVSWITTCWEASQERMVCGTDNEWLEKHMCPPLQGCMLSVTGLSTQERRDIQRLVTKHGGTYSGELKMNECTHLIVHKPHGQKYEFARKWNVACVTVQWLHDSVERGACQDESLYSVLP 287 HSP 2 Score: 92.4337 bits (228), Expect = 6.041e-19 Identity = 70/215 (32.56%), Postives = 108/215 (50.23%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRL-GGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWL-NETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWR---------EFGGK--AFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLNCTNYPK-----LQLPFEY-HWIKINFILSYLVDD 3970
R F L+ +E +I +L GG VL+ D +H+I RN+K+L+++A+GKW++H SY+EAC R++ E YEWGSS + AN LA A RWR E G + AF W++IL +SK F+R+ SGG VL G + + E TH F+++ + LQ ++ H ++ +I +L+ D
Sbjct: 1298 RYFQLSSMSAQERIDYLQLIEQLEGGVVLDKQYFDPSCSHIIVGNPLRNEKYLASMAAGKWVLHKSYLEACRAMGRFVQQEEEYEWGSSHILDAL-ANLTTQQRRLATAAMRWRKKLQLQRRQEPGAQVGAFGGWKVILNIEQSKESGFRRLLQSGGAEVLSSGEAQ-ALEEATHLFVDVARLRSEEVRTGLLQAAAQHVHCLRPEYIADFLIMD 1510 HSP 3 Score: 73.9442 bits (180), Expect = 2.350e-13 Identity = 60/238 (25.21%), Postives = 111/238 (46.64%), Query Frame = 2
Query: 2201 GLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPT-VVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI-------VSNQNYMISQSAKKSELETPQSESVKVETQINNPIE 2881
G+F+G F S S L +++E G++ P NSR + A + C E+ ++VT ++ C+ ++L+++ + LF P V+ G P L CV+S+ F E D L + +LGA VQ+ ++ K++ AST ++ ++A+ ++ + W +P V WLLE + A R + S + M + + TP S+ +E++ P++
Sbjct: 577 GIFSGKR-FAVVGFSADEESELALLVSEHGGKML--PKNSRAVAHYAVVP-LLGCEVENTVHEVVTNTWLVMCVEQQKLLELNSNPLFTPVPVLEGHYP---LSACVLSISQFTGTERDSLIFLAERLGARVQEFFVRKANPKKNMHASTHLLVREASGCKFEAAIKWDLPAVGVGWLLESARKGCRAKEHRHTVRLDDTEGQSREQEMPNAAPVVDAAHTPTDSSLALESKFVTPLD 807
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Nematostella
Match: EDO38946 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBH5]) HSP 1 Score: 129.413 bits (324), Expect = 4.297e-30 Identity = 76/234 (32.48%), Postives = 118/234 (50.43%), Query Frame = 2
Query: 176 LESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQ-----IENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKY 862
+ S ++ F+G+ F YLKS + I GP +L C+ SLP + P++ L+ + + E+Q L + I MM GT FT S+ L+ V S KYS A + V+ P+W++ +W + DP + RFK PIF IC++G R +K + N NGG++ GEL N THL+ K KY +A +WN+ V W +D +++G+ E + Y
Sbjct: 1 MPDRSIYILDPFEGDHFGYLKSQKCRICGPQCVLSCLKTKTSLPNSSFPVYTASLREVHACCTSVPRKERQHLHDYIHMMGGTVMRDFTESVTHLIAGEVGSKKYSVACSLNKPVMLPEWVFTLWEDGKSSHVVGTDPAYD----RFKCPIFKGCTICVTGLDAQTRQEVKQLCNQNGGVYSGELNMNTCTHLLVNQPKGEKYEFARKWNIHCVSTQWFYDCIRNGFWVEESGY 230 HSP 2 Score: 93.5893 bits (231), Expect = 4.600e-19 Identity = 63/194 (32.47%), Postives = 95/194 (48.97%), Query Frame = 2
Query: 3365 SSTSAPR----LFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWR--------------EFGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPP---VNEVTHSFINL 3883
S T PR F L+ +E S +I +LGG+V ++ + TH++ + RN+K+L+A+A+GKW++H SY+EA E ++ E +EWG +A + LA AKRWR G AF W + L +S+ FKR+ +GG V+ SS PP VTH+F+N
Sbjct: 1167 SPTEPPRPDVYKFQLSAMTPQEKADYSLLIEQLGGKVFDTMYFNPECTHVVVGKPNRNEKYLAAMAAGKWVLHKSYLEASREEGVFVREAPHEWGVE-----VAGEQPNK---LATAAKRWRCRLAHQRKTADPSQSLG--AFTGWSVFLCVDKSRQPGFKRLLEAGGAKVV---SSRPPYSCAEGVTHAFVNF 1347 HSP 3 Score: 54.299 bits (129), Expect = 3.542e-7 Identity = 35/138 (25.36%), Postives = 67/138 (48.55%), Query Frame = 2
Query: 2378 KLVTEYYISQCIANDRILDVDGDFLFYPTVV-AGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQN 2788
++VT ++ +C+ + ++D D FLF P V+ G +P L GCVI++ + E D + + + LGA Q+ + + S +AST ++ K+ + + W +WL C +L F ++ ++N
Sbjct: 559 QMVTFCWLEKCLEVEEVVDPDSCFLFRPFVIPGGAHP---LTGCVITISQYIGVERDHMMQLAELLGAIYQEKFAR--VNSASCQASTHLICKEPEGSKYAAAKKWKKYATTCNWLFACAKTGELVPVEDFPVLGSEN 691
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Nematostella
Match: EDO41864 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2Z1]) HSP 1 Score: 61.2326 bits (147), Expect = 9.865e-11 Identity = 40/142 (28.17%), Postives = 71/142 (50.00%), Query Frame = 2
Query: 3494 THLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWREFGGK---AFKDWRXXXXX-XXXXXXXFKRIFVSGGGTVL---PIGSSLPPV-NEVTHSFI--NLNC 3889
TH++CN+ R +KFL A+GKW++ Y+E +WL+E LYEW + R D + + RWR+ K F W ++++ + ++KR+ +G +L P + +P + ++TH F+ +L C
Sbjct: 5 THIVCNKPNRGEKFLCGCATGKWLLRKEYLEDSFAAQQWLDEELYEWNDACN----VPGLRQDHVSAPM---RWRKLSEKERGPFDGWNVLVLVNDAKRRIAYKRLLAAGQARILNGKPGDTYMPKLAKKLTHVFVEKSLEC 139
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Nematostella
Match: EDO29777 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2N6]) HSP 1 Score: 46.595 bits (109), Expect = 4.898e-6 Identity = 33/128 (25.78%), Postives = 60/128 (46.88%), Query Frame = 2
Query: 2408 CIANDRILDVDGDFLFYPTVV-AGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQN 2788
C+ + ++D D FLF P V+ G +P L GCVI++ + E D + + + LGA Q+ + + S +AST ++ K+ + + W +WL C +L F ++ ++N
Sbjct: 1 CLEVEEVVDPDSCFLFRPFVIPGGAHP---LTGCVITISQYIGVERDHMMQLAEMLGAIYQEKFAR--VNSASCQASTHLICKEPEGSKYAAAKKWKKYATTCNWLFACAKTGELVPVEDFPVLGSEN 123
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Medaka
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:NCBI gene;Acc:101166667]) HSP 1 Score: 131.724 bits (330), Expect = 2.643e-30 Identity = 77/259 (29.73%), Postives = 132/259 (50.97%), Query Frame = 2
Query: 92 KAYDSLLEFEDVKAVK-LSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIW-NSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKY 862
KAY+++LE + K +K + E ++ EK S FVFS F AF + K I P ++LYC+ Q +PK P++N + + IS + L+ + +++L+++M G S+ L+ V S KY A N +L P W+ W SQ + + + + P+ +C++G ER ++ + +G + G+L NE THLI +KY A +WNV V ++WL DS++ G+C++ ++Y
Sbjct: 26 KAYEAILELQSEKYLKKVKEEEVLQMEEK--DKSLFVFSSFTSPAFLHCKKHGCRIVSPLVVLYCLQQQRCVPKAEKPVYNMAMADVTISCTSLEKAARVDVMDLVQLMGGRACLDLNVSVTHLIAGEVGSKKYLVAANLGKPILLPSWVKACWEKSQNSLFRYTDLPMDEYLCPVLLGCTVCVTGLSSTERKEVQRLCEQHGAKYSGQLKMNECTHLIVSEPAGQKYECARKWNVYCVSLHWLFDSIEKGFCQDESRY 282 HSP 2 Score: 97.8265 bits (242), Expect = 5.198e-20 Identity = 63/179 (35.20%), Postives = 91/179 (50.84%), Query Frame = 2
Query: 3380 PRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSS----GTNTLIAANDRDDLLPLALCAKRWREF--GGK----AFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN 3886
P F ++ +E S +I LGG VLE D +H+I RN+K+L+A+A+GKWI+H SY+E C R++ E +EWGSS ++ R LA A RWR+ G + AF W ++L +S+ F+R+ SGG VLP G S E TH F + +
Sbjct: 1215 PPRFQMSSLSPQERIDYSHVIEDLGGVVLEKQSFDPSCSHIIVGSPLRNEKYLAAMAAGKWILHRSYLEVCRGVGRFIQEDDFEWGSSCILDALPSITLQQRR-----LATAAMRWRKALQGSREREGAFSGWTVMLNIDQSRESGFRRLLQSGGAKVLP-GPSPSLYREATHLFADFS 1387 HSP 3 Score: 67.0106 bits (162), Expect = 1.656e-10 Identity = 87/409 (21.27%), Postives = 162/409 (39.61%), Query Frame = 2
Query: 1721 GLSGEIINNRIKFVDKSWLVQSIEKWELLPVDGGFLI---ESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVV--DDDWALSIDSRFTY----VNKSWLEQSIHAGK-----------CLPVDIFLVTDSNGIDLSIDDQSPWISESTIQQE-------TRIIDSNSTTD--------------------------GLFNG-ILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPES---KLVTEYYISQCIANDRILDVDGDFLFYPTVV-AGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLI 2773
GLS ++ + D + + +E + P+DG L ++ + C + G + K ++N+ G ++ +T H+V+ D S+ Y V WL S G+ CLP D V + + + P S ST +Q ++ +D + T GLF+G +F+ + + ++L ++ E G++ R + A + C E+ ++VT+ +++ C+ + +L + + LF P V G P L GCV+S+ F E + L + + +GA VQD ++ + K + AST +V + + ++ W +P V+ W+LE Q A FL+
Sbjct: 313 GLSNISVSGSMAINDTALTISRLETAD--PIDGLDLTVCPADDLLDGCKLYLCGLPLKKLEKLRRLVNNAGGLRFNQPSEELT---HVVMGEPDQELTKFLSKAAYRPHVVTVQWLLDSYSRGRLLPEAEYLHPDCLPPDSASVPAGHVVKPRLSAGPPVASPSTPRQNRLEEDLLSQYMDDDPTVGKNLFPQAPSLTRGPEQDSALQEFSETGLFSGKSFLFVGFGVE--AEAQLSTLVIEHGGKVLAG--RCRAVADYAVVP-LLGCSVEATVDEVVTDTWLAMCVEKECVLLLSSNPLFTPVAVREGCFP---LEGCVLSVSQFIGAERESLIQLAKHVGADVQDYFVRFAIKKKGMVASTHLVLQSPDGTKFQAAKKWGLPAVSMHWILESARTGQKAAEEMFLV 708
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Medaka
Match: ect2 (epithelial cell transforming 2 [Source:NCBI gene;Acc:101157762]) HSP 1 Score: 63.1586 bits (152), Expect = 1.955e-9 Identity = 40/164 (24.39%), Postives = 76/164 (46.34%), Query Frame = 2
Query: 179 ESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQK-LINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPER 661
E + FV ++F + +L + I GP ++ +C +++ + + PL+ + N + + E+ K L+NL+ M GT + F+ + L+ + KY A +L+P WI W + + D E++ FK+P F + + GF E E+
Sbjct: 124 EFETVFVLTDFDTSDYSFLHRRDNRIVGPPVVFHCAAKEEPMLFSCRPLYCTAMLNLSLCLTGFRNKEEMKNLVNLVHHMGGTIRKDFSTKVTHLVAYSTHGEKYRLAVCMGTPILTPLWIQKSWERRDDVDFHAGQEEFRSEFKVPPFQDCVLSFLGFSEEEK 287
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Medaka
Match: ect2 (epithelial cell transforming 2 [Source:NCBI gene;Acc:101157762]) HSP 1 Score: 62.7734 bits (151), Expect = 2.672e-9 Identity = 40/164 (24.39%), Postives = 76/164 (46.34%), Query Frame = 2
Query: 179 ESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQK-LINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPER 661
E + FV ++F + +L + I GP ++ +C +++ + + PL+ + N + + E+ K L+NL+ M GT + F+ + L+ + KY A +L+P WI W + + D E++ FK+P F + + GF E E+
Sbjct: 124 EFETVFVLTDFDTSDYSFLHRRDNRIVGPPVVFHCAAKEEPMLFSCRPLYCTAMLNLSLCLTGFRNKEEMKNLVNLVHHMGGTIRKDFSTKVTHLVAYSTHGEKYRLAVCMGTPILTPLWIQKSWERRDDVDFHAGQEEFRSEFKVPPFQDCVLSFLGFSEEEK 287
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Medaka
Match: ect2 (epithelial cell transforming 2 [Source:NCBI gene;Acc:101157762]) HSP 1 Score: 62.7734 bits (151), Expect = 2.791e-9 Identity = 40/164 (24.39%), Postives = 76/164 (46.34%), Query Frame = 2
Query: 179 ESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQK-LINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPER 661
E + FV ++F + +L + I GP ++ +C +++ + + PL+ + N + + E+ K L+NL+ M GT + F+ + L+ + KY A +L+P WI W + + D E++ FK+P F + + GF E E+
Sbjct: 93 EFETVFVLTDFDTSDYSFLHRRDNRIVGPPVVFHCAAKEEPMLFSCRPLYCTAMLNLSLCLTGFRNKEEMKNLVNLVHHMGGTIRKDFSTKVTHLVAYSTHGEKYRLAVCMGTPILTPLWIQKSWERRDDVDFHAGQEEFRSEFKVPPFQDCVLSFLGFSEEEK 256
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Medaka
Match: ect2 (epithelial cell transforming 2 [Source:NCBI gene;Acc:101157762]) HSP 1 Score: 62.7734 bits (151), Expect = 2.913e-9 Identity = 40/164 (24.39%), Postives = 76/164 (46.34%), Query Frame = 2
Query: 179 ESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQK-LINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIEND--PFNEQYLKRFKIPIFFNLKICLSGFLEPER 661
E + FV ++F + +L + I GP ++ +C +++ + + PL+ + N + + E+ K L+NL+ M GT + F+ + L+ + KY A +L+P WI W + + D E++ FK+P F + + GF E E+
Sbjct: 124 EFETVFVLTDFDTSDYSFLHRRDNRIVGPPVVFHCAAKEEPMLFSCRPLYCTAMLNLSLCLTGFRNKEEMKNLVNLVHHMGGTIRKDFSTKVTHLVAYSTHGEKYRLAVCMGTPILTPLWIQKSWERRDDVDFHAGQEEFRSEFKVPPFQDCVLSFLGFSEEEK 287
BLAST of DNA topoisomerase 2 binding protein 1 vs. Planmine SMEST
Match: SMESG000035281.1 (SMESG000035281.1) HSP 1 Score: 2577.74 bits (6680), Expect = 0.000e+0 Identity = 1335/1335 (100.00%), Postives = 1335/1335 (100.00%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIIDNKVGLITKNYGNYVDKSWLEKSINNGKRLPLKEFLVLGEIPNEVSSDFYSPICGDIFSNCLIMFHGFSNEFIDKWTKXXXXXXXXXXXXKQPSQQQNPTHVVVRSSENLPIKSQVSYVTENWLEQSIFHRKQMPVRDFQFPLVSSDDELFSADEYDNDCDSQSFLSNDNTTSGNNQPSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEIINNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGIDLSIDDQSPWISESTIQQETRIIDSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQNYMISQSAKKSELETPQSESVKVETQINNPIENDESNHHQNGAVITKSISKVSNVMTRSLKRKNNAHISLEIELEAEHSDPAAPLDQEMVPREIGWNYEASNDNTNLSENNIQIKTEIVEDNPKRMRRXXXXXXXXXXXEDPITADKTLRKQIKAEPKDKMVSEVXXXXXXXXXXXXXXXXXXXXXXXXGRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWREFGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLNCTNYPKLQLPFEYHWIKINFILSYLVDDGTNLNSINSSYIYTKTQISDFNKRMQSKVKAKK 4069
NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTIKNIINNNGGIHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIIDNKVGLITKNYGNYVDKSWLEKSINNGKRLPLKEFLVLGEIPNEVSSDFYSPICGDIFSNCLIMFHGFSNEFIDKWTKIIEMSSGGIISSKQPSQQQNPTHVVVRSSENLPIKSQVSYVTENWLEQSIFHRKQMPVRDFQFPLVSSDDELFSADEYDNDCDSQSFLSNDNTTSGNNQPSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEIINNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGIDLSIDDQSPWISESTIQQETRIIDSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQNYMISQSAKKSELETPQSESVKVETQINNPIENDESNHHQNGAVITKSISKVSNVMTRSLKRKNNAHISLEIELEAEHSDPAAPLDQEMVPREIGWNYEASNDNTNLSENNIQIKTEIVEDNPKRMRRISVSISPIVISEDPITADKTLRKQIKAEPKDKMVSEVKKAKAKIADKKPKTKEVTVAVKEVGRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWREFGGKAFKDWRIILIESESKIESFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLNCTNYPKLQLPFEYHWIKINFILSYLVDDGTNLNSINSSYIYTKTQISDFNKRMQSKVKAKK
Sbjct: 12 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTIKNIINNNGGIHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIIDNKVGLITKNYGNYVDKSWLEKSINNGKRLPLKEFLVLGEIPNEVSSDFYSPICGDIFSNCLIMFHGFSNEFIDKWTKIIEMSSGGIISSKQPSQQQNPTHVVVRSSENLPIKSQVSYVTENWLEQSIFHRKQMPVRDFQFPLVSSDDELFSADEYDNDCDSQSFLSNDNTTSGNNQPSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEIINNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGIDLSIDDQSPWISESTIQQETRIIDSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQNYMISQSAKKSELETPQSESVKVETQINNPIENDESNHHQNGAVITKSISKVSNVMTRSLKRKNNAHISLEIELEAEHSDPAAPLDQEMVPREIGWNYEASNDNTNLSENNIQIKTEIVEDNPKRMRRISVSISPIVISEDPITADKTLRKQIKAEPKDKMVSEVKKAKAKIADKKPKTKEVTVAVKEVGRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWREFGGKAFKDWRIILIESESKIESFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLNCTNYPKLQLPFEYHWIKINFILSYLVDDGTNLNSINSSYIYTKTQISDFNKRMQSKVKAKK 1346
BLAST of DNA topoisomerase 2 binding protein 1 vs. Planmine SMEST
Match: SMESG000035281.1 (SMESG000035281.1) HSP 1 Score: 2577.36 bits (6679), Expect = 0.000e+0 Identity = 1335/1335 (100.00%), Postives = 1335/1335 (100.00%), Query Frame = 2
Query: 65 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIIDNKVGLITKNYGNYVDKSWLEKSINNGKRLPLKEFLVLGEIPNEVSSDFYSPICGDIFSNCLIMFHGFSNEFIDKWTKXXXXXXXXXXXXKQPSQQQNPTHVVVRSSENLPIKSQVSYVTENWLEQSIFHRKQMPVRDFQFPLVSSDDELFSADEYDNDCDSQSFLSNDNTTSGNNQPSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEIINNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGIDLSIDDQSPWISESTIQQETRIIDSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQNYMISQSAKKSELETPQSESVKVETQINNPIENDESNHHQNGAVITKSISKVSNVMTRSLKRKNNAHISLEIELEAEHSDPAAPLDQEMVPREIGWNYEASNDNTNLSENNIQIKTEIVEDNPKRMRRXXXXXXXXXXXEDPITADKTLRKQIKAEPKDKMVSEVXXXXXXXXXXXXXXXXXXXXXXXXGRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWREFGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLNCTNYPKLQLPFEYHWIKINFILSYLVDDGTNLNSINSSYIYTKTQISDFNKRMQSKVKAKK 4069
NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTIKNIINNNGGIHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIIDNKVGLITKNYGNYVDKSWLEKSINNGKRLPLKEFLVLGEIPNEVSSDFYSPICGDIFSNCLIMFHGFSNEFIDKWTKIIEMSSGGIISSKQPSQQQNPTHVVVRSSENLPIKSQVSYVTENWLEQSIFHRKQMPVRDFQFPLVSSDDELFSADEYDNDCDSQSFLSNDNTTSGNNQPSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEIINNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGIDLSIDDQSPWISESTIQQETRIIDSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQNYMISQSAKKSELETPQSESVKVETQINNPIENDESNHHQNGAVITKSISKVSNVMTRSLKRKNNAHISLEIELEAEHSDPAAPLDQEMVPREIGWNYEASNDNTNLSENNIQIKTEIVEDNPKRMRRISVSISPIVISEDPITADKTLRKQIKAEPKDKMVSEVKKAKAKIADKKPKTKEVTVAVKEVGRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWREFGGKAFKDWRIILIESESKIESFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLNCTNYPKLQLPFEYHWIKINFILSYLVDDGTNLNSINSSYIYTKTQISDFNKRMQSKVKAKK
Sbjct: 11 NDSPSPMFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTIKNIINNNGGIHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEFNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIIDNKVGLITKNYGNYVDKSWLEKSINNGKRLPLKEFLVLGEIPNEVSSDFYSPICGDIFSNCLIMFHGFSNEFIDKWTKIIEMSSGGIISSKQPSQQQNPTHVVVRSSENLPIKSQVSYVTENWLEQSIFHRKQMPVRDFQFPLVSSDDELFSADEYDNDCDSQSFLSNDNTTSGNNQPSLLFDNCWILFHGCQKNDIDYWKSIIENNSGRVVRKLSDFSTIADGKRLVVVVDCREGLSGEIINNRIKFVDKSWLVQSIEKWELLPVDGGFLIESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSITPPTHIVVDDDWALSIDSRFTYVNKSWLEQSIHAGKCLPVDIFLVTDSNGIDLSIDDQSPWISESTIQQETRIIDSNSTTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSNQNYMISQSAKKSELETPQSESVKVETQINNPIENDESNHHQNGAVITKSISKVSNVMTRSLKRKNNAHISLEIELEAEHSDPAAPLDQEMVPREIGWNYEASNDNTNLSENNIQIKTEIVEDNPKRMRRISVSISPIVISEDPITADKTLRKQIKAEPKDKMVSEVKKAKAKIADKKPKTKEVTVAVKEVGRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDDLLPLALCAKRWREFGGKAFKDWRIILIESESKIESFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLNCTNYPKLQLPFEYHWIKINFILSYLVDDGTNLNSINSSYIYTKTQISDFNKRMQSKVKAKK 1345
BLAST of DNA topoisomerase 2 binding protein 1 vs. Planmine SMEST
Match: SMESG000021513.1 (SMESG000021513.1) HSP 1 Score: 162.925 bits (411), Expect = 3.950e-40 Identity = 108/380 (28.42%), Postives = 184/380 (48.42%), Query Frame = 2
Query: 194 FVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWN---SQIENDPFNEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYEF--------------------------------------------------NPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEKTTHFIIDNKVGLITKNYGN-YVDKSWLEKSINNGKRLPLKEFLVLGEIPNEVSSDFYSP 1171
FV F+ + +YL+SL+ IFGP +L +D LPK+ PLF+ LK+ + S ++ K L N +KMM G S+ ++QIL++ +V+S KY A I V+ +W+ ++W + ++ + ++ + +++IPI NLKICLSGF E ER+ K ++N NGG ++ L N+ THLI K + +K+SYA +WN+ V WL +S+K GY + ++Y+ + I+ C + C +EF+ +W+ +I+ N GK+ ++ TH I+ + L K G Y+ WL+ +N + + + +++V D YSP
Sbjct: 2 FVLGYFECSSLKYLESLKLPIFGPQCLLAYFGKDDVLPKSPYPLFSIALKDIHVCFSSIEEENKIDLTNKVKMMHGFVSDALKANVQILISSSVQSRKYRVASEKGIPVVRKEWVEELWTLSKNCVQLNGVDQILIDKYQIPILHNLKICLSGFNESERDEAKQMVNENGGSYYPNLNVNDCTHLIAKNASGQKFSYAKKWNITTVTCEWLIESIKKGYACDVDQYQLSDEIHVPGSTPKKDSKIIMDSKCNEISVIFDGSHADETRLDNHDESDCVPDSRIWIDCHFQLLDCKEEFVTKWENLIKENGGKIV--SSVTMPPTHIIVGDCTKLTEKTDGILYLKSDWLQACLNENRLVNVHQYIV----------DIYSP 369 HSP 2 Score: 127.487 bits (319), Expect = 3.120e-29 Identity = 93/336 (27.68%), Postives = 164/336 (48.81%), Query Frame = 2
Query: 1832 ESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISESEFSIT-PPTHIVVDDDWALSIDSR-FTYVNKSWLEQSIHAGKCLPVDIFLV----------TDSNGI--DLSIDDQSPWISESTIQQETRIID-SNSTTDGLFNGIL---VFIDQSISDYSSSRLVKIITEKSGE-ITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQLADYSRFLIVSN 2782
+S ++ +C C + F+ KW +I G+++S S+T PPTHI+V D L+ + Y+ WL+ ++ + + V ++V +D+ G +L D + + + E+ +I+ S + D + NGI +F + + S R+V ITE +G I+ ++ I+ F +++VT YYI QC+ ++IL +D DFLF P + + +LVG VIS G+ + E L+ KLGATVQD + + +K + A+T ++ ++ S W IP V + W+ EC+ R + D +L+ S+
Sbjct: 280 DSRIWIDCHFQLLDCKEEFVTKWENLIKENGGKIVS----SVTMPPTHIIVGDCTKLTEKTDGILYLKSDWLQACLNENRLVNVHQYIVDIYSPAANVQSDNWGSMNELDWGDTMKTVEVNNNKDESTVIEKSTMSIDAIPNGIFYKFIFHMDNETCEKSIRIVNSITENAGTVISTTEGSTHYILPNIFSTPIKSISSSTRVVTVYYIEQCLEQNQILPLDSDFLFNPISIDSNHYNTILVGTVISFSGYPAEERQLLKEICTKLGATVQDQMSRKKIPSKRILATTHLIISKPDNKKFPSSKSWGIPAVTKHWIGECINRLKRVDPVNYLLQSD 611 HSP 3 Score: 114.775 bits (286), Expect = 2.403e-25 Identity = 71/225 (31.56%), Postives = 112/225 (49.78%), Query Frame = 2
Query: 3383 RLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLIAANDRDD----LLPLALCAKRWREFGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNEVTHSFINLN-CTNYPKLQLPFEYHWIKINFILSYL-VDDGTNLNSINSSYIYTKTQISDFNK 4039
++F+ + + I LGG + ++ THLIC N+K+L+A+A+GKW++H SY++ + +WL ET ++WG S N + N L L +K W E GG AFK+WR+ LIE K E +I +GGGTV+P + + VTH +N + C+ P L +Y KI I S+L + + N +I + ++ F K
Sbjct: 945 KVFMFSNIAKTLEQHYDEEIRNLGGSLELNSDFSMRMTHLICGEPCLNEKYLAAIAAGKWVLHKSYLDESISQGKWLEETEFQWGGSRKNEFLQLNPTKTYGKIFLALLDASKHWHEIGGGAFKEWRVCLIEKNEKYEKMCKIIEAGGGTVVPFSEPISQLG-VTHFLVNKSQCSGLPDLS---QYPCYKIEMIPSFLSAQNDREIKRCNKKFIVSAAEMQKFRK 1165
BLAST of DNA topoisomerase 2 binding protein 1 vs. Planmine SMEST
Match: SMESG000012344.1 (SMESG000012344.1) HSP 1 Score: 150.599 bits (379), Expect = 1.517e-36 Identity = 113/398 (28.39%), Postives = 192/398 (48.24%), Query Frame = 2
Query: 68 DSPSPMFDKAYDSL--LEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPF---NEQYLKRFKIPIFFNLKICLSGFLEPERNTXXXXXXXXXXXHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYE--------------------------------FNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEK--TTHFIIDNKVGLITKNYG-NYVDKSWLEKSINNGKRLPLKEFLVLGEIP 1141
D+ +P+ +AY++L +E ++ +K + S + K + ++F F+ ++ YLKS + FGP +L + D +LP +PLF+ LKN ++ + + K++L + + MM GT S+ S +IL++ ++ + KY +A I V+S DWI IW DP +E ++++++IPIF NL+I L+GF ER I+ II GG + +LT THLI + K+ A +W + V WL DS++ GY + KY+ N I++ C+I F+ C EF+ +W +I G+ + N + TH I+ + L K YV+ WL+ ++ K+F + EIP
Sbjct: 11 DTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNK---HCSYIFGYFEEKSIYYLKSHKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILEREIIEKIIIKCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFDI--EIP 403 HSP 2 Score: 114.775 bits (286), Expect = 2.269e-25 Identity = 74/209 (35.41%), Postives = 109/209 (52.15%), Query Frame = 2
Query: 3353 GRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLI-----AANDRDDLLPLALCAKRWREFGGKAFKDWRXXXXXXXXXXXXFKRIFVSGGGTVLPIGSSLPPVNE--VTHSFINLN-CTNYPKLQLPFEYHWIKINFILS 3955
G + + ++F+L L + + +LGG + + FTHL+ R R +K+L+A+A+GKW++H+SYI+ RW++E Y+WG T+ I A L LA AKR+ + GG +F+DWR+ L+E K E RI SGGGT++P+ + PV E VTH IN N C+ P L Y I F LS
Sbjct: 679 GAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWIDEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPL---IEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLIPFYLS 884 HSP 3 Score: 97.8265 bits (242), Expect = 3.166e-20 Identity = 88/334 (26.35%), Postives = 154/334 (46.11%), Query Frame = 2
Query: 1829 IESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISE-SEFSITPPTHIVVDDDWALS-IDSRFTYVNKSWLEQSIHAGKCL----------PVDIFLVTDSNGI-DLSIDDQSPW--ISESTIQQETRIIDSNS----------TTDGLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPE--SKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDELRRYSMKLGATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQL 2749
+ + ++++C+I F C F+ KW+ +I G ++ S+ THI+V D L + TYVN WL Q+ GK L P V SN I DLSI+ W + + + + DSNS + G+F+ F+ ++S + +I SG I +P + IV L + P+ S LVT +Y+++C+ + ++L ++ DF+F INP +L G ++S G+ E L+ GATVQD + + +K++ A+T ++ + S W+IP V + W+ +C+ +++
Sbjct: 311 LNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWL-QACLDGKALSDSKQFDIEIPKSPIAVPISNPIPDLSIN----WDLMDQYNLGNVSNPFDSNSERINNENINPNSFGIFHKYRFFL-HNLSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLC---RSPPQECSSLVTVFYLTECVKHKQLLSIESDFMF---TRMDINPNIL-TGTILSFSGYSVDERSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLIISKPDKKKFPSSKIWNIPAVTKHWIGDCIAHREM 631
BLAST of DNA topoisomerase 2 binding protein 1 vs. Planmine SMEST
Match: SMESG000002680.1 (SMESG000002680.1) HSP 1 Score: 52.7582 bits (125), Expect = 2.624e-6 Identity = 31/108 (28.70%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 3380 PRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLY---------EWGSSGTNTLIAANDR 3676
P + TG + KL+D++ +GGEV + ++R FTHLI + R KF + G I+ + +I+AC +++ E L+ ++G S +L+ A +R
Sbjct: 1187 PIIVAFTGLIPEQRDKLTDLVISIGGEVTDD--MNR-FTHLIAKNIVRTPKFYFGILRGCQIVSAKWIQACAYRGQFIEEKLWRLEDKEGERQYGFSLHESLLKAQNR 1291 The following BLAST results are available for this feature:
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of DNA topoisomerase 2 binding protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA topoisomerase 2 binding protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of DNA topoisomerase 2 binding protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of DNA topoisomerase 2 binding protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30009244 ID=SMED30009244|Name=DNA topoisomerase 2 binding protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=4069bpback to top protein sequence of SMED30009244-orf-1 >SMED30009244-orf-1 ID=SMED30009244-orf-1|Name=SMED30009244-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1329bp MFDKAYDSLLEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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