Radial spoke head 14-like protein

Overview


Name	Radial spoke head 14-like protein
Smed ID	SMED30009188
Length (bp)	1180

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Radial spoke head 14-like protein (SMED30009188) t-SNE clustered cells

Violin plots show distribution of expression levels for Radial spoke head 14-like protein (SMED30009188) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Radial spoke head 14-like protein (SMED30009188) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Radial spoke head 14-like protein (SMED30009188) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30009188

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30009188	SMESG000077061.1	SmedASXL_005396	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30009188	SMESG000077061.1	Contig45549	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30009188	SMESG000077061.1	Contig45549	newmark_ests	PMID:29674431 Fincher et al., 2018
head region	SMED30009188	SMESG000077061.1	dd_Smed_v6_1917_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
epidermis	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ciliated epithelial cell	SMED30009188	SMESG000077061.1	dd_Smed_v4_1917_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Radial spoke head 14-like protein vs. Ensembl Human
Match: RSPH14 (radial spoke head 14 homolog [Source:HGNC Symbol;Acc:HGNC:13437])

HSP 1 Score: 177.563 bits (449), Expect = 1.332e-51
Identity = 129/348 (37.07%), Postives = 203/348 (58.33%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKV--QEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTL------DAVAQHAKIAVDVITWKP 1109
            MA  Q S E P +I+ T+ T AYG RA+P+L  EL S DL TRQKAL AL D +HD E I  ++N G + +++ LL+  +   R    EV ++ + HSVGR A LE+ +++ L  L +D   + R  +YK      +   GA+ II+  LI  L  K+  + E +E + +IL+ L  CL  D  +AL +  + +L   L      I++KAA+ ++++++S EGK+     + +P L+ +LK     VK++AAGA+    +IT GKYAA+   A+  LL L++ P +  R+N+ KALT +AE PEGR+ L  H+  F+   ++T       +A+ + A+IA+ VI +KP
Sbjct:    1 MAHSQNSLELPININATQITTAYGHRALPKLKEELQSEDLQTRQKALMALCDLMHDPECIYKAMNIGCMENLKALLKDSNSMVRIKTTEVLHITASHSVGRYAFLEHDIVLALSFLLNDPSPVCRGNLYKAYMQLVQVPRGAQEIISKGLISSLVWKLQVEVEEEEFQEFILDTLVLCLQEDATEALGSNVVLVLKQKLLSANQNIRSKAARALLNVSISREGKKQVCHFDVIPILVHLLKDPVEHVKSNAAGALMFATVITEGKYAALEAQAIGLLLELLHSPMTIARLNATKALTMLAEAPEGRKALQTHVPTFRAMEVETYEKPQVAEALQRAARIAISVIEFKP 348

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BLAST of Radial spoke head 14-like protein vs. Ensembl Human
Match: RSPH14 (radial spoke head 14 homolog [Source:HGNC Symbol;Acc:HGNC:13437])

HSP 1 Score: 83.9593 bits (206), Expect = 3.087e-19
Identity = 46/101 (45.54%), Postives = 63/101 (62.38%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGR 392
            MA  Q S E P +I+ T+ T AYG RA+P+L  EL S DL TRQKAL AL D +HD E I  ++N G + +++ LL+  +   R    EV ++ + HSVGR
Sbjct:    1 MAHSQNSLELPININATQITTAYGHRALPKLKEELQSEDLQTRQKALMALCDLMHDPECIYKAMNIGCMENLKALLKDSNSMVRIKTTEVLHITASHSVGR 101

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BLAST of Radial spoke head 14-like protein vs. Ensembl Zebrafish
Match: rsph14 (radial spoke head 14 homolog [Source:ZFIN;Acc:ZDB-GENE-081104-375])

HSP 1 Score: 174.866 bits (442), Expect = 6.312e-51
Identity = 122/340 (35.88%), Postives = 187/340 (55.00%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA  Q + +PPP ID T+A +A+G  A+P+L  EL   DL TRQKAL AL D LHD E    ++    +  ++ LL+  D   R    EV YV + HS+GR A L   ++  L  L  D E+  RK V++ L   +E+  GA  ++N  L+P L  K+  E + ++  +L  L  CL ++   AL++  I++L   L H    I+ +AA  ++ I++   GK    +E  +P L+++L   +  V A+AAG I N A+IT GK  A+   AL  LL L+   N+ V  N+++ALT +AE P  R  L  H+ L K R+ D    + + A   ++VI+W+P
Sbjct:    1 MASPQTTEQPPPHIDPTQAPIAFGNLALPKLLVELQDDDLFTRQKALTALCDLLHDPERAYEAIFSECLEILKVLLQDEDSLIRVKTTEVLYVLASHSMGRDAFLNFDIVGPLANLLDDPEIACRKNVHRALNRIAEFPLGAVFMVNKGLVPRLVPKISIEEENIRALVLSTLSCCLSVNALPALESDVIQVLRDQLSHSSPIIRKEAASALMAISVPTVGKIKVCEENLLPILVKLLSDDDQEVVANAAGTIMNTAVITKGKSEALEADALAPLLQLVLSENTAVCANALRALTVLAEVPRARAHLRQHVPLLKTRLTDPNYLIQEAASTTIEVISWQP 340

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BLAST of Radial spoke head 14-like protein vs. Ensembl Zebrafish
Match: rsph14 (radial spoke head 14 homolog [Source:ZFIN;Acc:ZDB-GENE-081104-375])

HSP 1 Score: 124.405 bits (311), Expect = 5.198e-33
Identity = 73/199 (36.68%), Postives = 109/199 (54.77%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFH 686
            MA  Q + +PPP ID T+A +A+G  A+P+L  EL   DL TRQKAL AL D LHD E    ++    +  ++ LL+  D   R    EV YV + HS+GR A L   ++  L  L  D E+  RK V++ L   +E+  GA  ++N  L+P L  K+  E + ++  +L  L  CL ++   AL++  I++L   L H
Sbjct:    1 MASPQTTEQPPPHIDPTQAPIAFGNLALPKLLVELQDDDLFTRQKALTALCDLLHDPERAYEAIFSECLEILKVLLQDEDSLIRVKTTEVLYVLASHSMGRDAFLNFDIVGPLANLLDDPEIACRKNVHRALNRIAEFPLGAVFMVNKGLVPRLVPKISIEEENIRALVLSTLSCCLSVNALPALESDVIQVLRDQLSH 199

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BLAST of Radial spoke head 14-like protein vs. Ensembl Xenopus
Match: RSPH14 (radial spoke head 14 homolog (Chlamydomonas) [Source:NCBI gene;Acc:594888])

HSP 1 Score: 237.269 bits (604), Expect = 7.176e-75
Identity = 152/340 (44.71%), Postives = 223/340 (65.59%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA  +IS   PP+ID TK+ +A+G RA+P+L  EL   +L+TRQ+AL AL D +HD E++  +V+ G + S+++LL   D T R  A EV Y+ + HSVGR  +L++ VI  + KL  D  +I R+ ++KT EM SE   GA A++ ANL+  L  K++ E++++++ ILE LHF L  DV  AL    + IL   L H    I+  AAK ++DI +  EGKE   +EE VP L+ +L+  ++ V+A+AAGA+ NI I T GKYAAIN GA+P LL L++D  S VR+NS+KALT ++E PEGR++LL  +++ +  + D  +AV + A IA+ VI WKP
Sbjct:    1 MAQARISCCLPPNIDPTKSPVAFGERALPKLNEELKDSELITRQRALMALCDLVHDPENVYQAVHLGFLESLKSLLHDQDSTVRQKATEVFYIMAGHSVGRQGILKSDVIPAISKLLDDPVVICRQNMHKTFEMVSELPAGAAALVKANLVVQLVQKLECELEDIQMIILETLHFMLQTDVLQALSAGAVGILKGKLSHPSAGIRRMAAKALMDICVPLEGKETVCQEEVVPLLVHLLQDSDNEVRANAAGALMNITITTQGKYAAINSGAIPKLLALVSDGYSRVRLNSLKALTTLSEAPEGRKVLLADVNIIQSCLDDQSEAVRRAAAIAIQVIQWKP 340

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BLAST of Radial spoke head 14-like protein vs. Ensembl Mouse
Match: Rsph14 (radial spoke head homolog 14 (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1918486])

HSP 1 Score: 216.853 bits (551), Expect = 4.707e-67
Identity = 134/341 (39.30%), Postives = 215/341 (63.05%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLIN-DPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA  +IS   PPDID TKA +AYG RA+ +L  EL S DLLTRQKAL AL D +HD E++  ++N G + S++ LL+  D   R    EV Y+ + H VGR+  L++ +I  L  L SD + + R+ +++  +  ++   GA+ I+ + LIP L  K+Q+E D ++  IL+ L  CL  D  +AL++Q +  L   L  + +EI++KAA+ +I I++  +GK    K + +P L+ +L   +  VKA+AAGA+ +  + T GKYAA++  A+  LL L++ +P +++ +N+ KALT +AE PEGR+LLL H+ +F+  +    +A+ + A++A+ VI WKP
Sbjct:    1 MAHARISMYMPPDIDPTKAAIAYGCRALSKLNEELQSRDLLTRQKALVALCDLMHDPEYVYEAINIGCLESLKTLLQDDDNLVRIKTTEVLYIMATHYVGRVGFLKHDIIQALSLLLSDHQTLCRENLHQAYKHLAQLPKGAQGIVQSGLIPSLVRKLQKEEDHIQEIILDTLALCLQEDATEALESQAVPCLKEKLLSQNSEIRSKAARALIAISIPLDGKNQVWKNKVIPILVTLLSDTDEEVKANAAGALMHATVTTEGKYAALDANAIEPLLELLSTNPKTKLCLNATKALTMLAEAPEGRKLLLSHVPIFRYLLAHKNEAIQRAAEVAIKVIEWKP 341

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Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLIN-DPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA  +IS   PPDID TKA +AYG RA+ +L  EL S DLLTRQKAL AL D +HD E++  ++N G + S++ LL+  D   R    EV Y+ + H VGR+  L++ +I  L  L SD + + R+ +++  +  ++   GA+ I+ + LIP L  K+Q+E D ++  IL+ L  CL  D  +AL++Q +  L   L  + +EI++KAA+ +I I++  +GK    K + +P L+ +L   +  VKA+AAGA+ +  + T GKYAA++  A+  LL L++ +P +++ +N+ KALT +AE PEGR+LLL H+ +F+  +    +A+ + A++A+ VI WKP
Sbjct:    1 MAHARISMYMPPDIDPTKAAIAYGCRALSKLNEELQSRDLLTRQKALVALCDLMHDPEYVYEAINIGCLESLKTLLQDDDNLVRIKTTEVLYIMATHYVGRVGFLKHDIIQALSLLLSDHQTLCRENLHQAYKHLAQLPKGAQGIVQSGLIPSLVRKLQKEEDHIQEIILDTLALCLQEDATEALESQAVPCLKEKLLSQNSEIRSKAARALIAISIPLDGKNQVWKNKVIPILVTLLSDTDEEVKANAAGALMHATVTTEGKYAALDANAIEPLLELLSTNPKTKLCLNATKALTMLAEAPEGRKLLLSHVPIFRYLLAHKNEAIQRAAEVAIKVIEWKP 341

[Close]

BLAST of Radial spoke head 14-like protein vs. Ensembl Mouse
Match: Rsph14 (radial spoke head homolog 14 (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1918486])

HSP 1 Score: 216.853 bits (551), Expect = 1.501e-66
Identity = 134/341 (39.30%), Postives = 215/341 (63.05%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLIN-DPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA  +IS   PPDID TKA +AYG RA+ +L  EL S DLLTRQKAL AL D +HD E++  ++N G + S++ LL+  D   R    EV Y+ + H VGR+  L++ +I  L  L SD + + R+ +++  +  ++   GA+ I+ + LIP L  K+Q+E D ++  IL+ L  CL  D  +AL++Q +  L   L  + +EI++KAA+ +I I++  +GK    K + +P L+ +L   +  VKA+AAGA+ +  + T GKYAA++  A+  LL L++ +P +++ +N+ KALT +AE PEGR+LLL H+ +F+  +    +A+ + A++A+ VI WKP
Sbjct:   34 MAHARISMYMPPDIDPTKAAIAYGCRALSKLNEELQSRDLLTRQKALVALCDLMHDPEYVYEAINIGCLESLKTLLQDDDNLVRIKTTEVLYIMATHYVGRVGFLKHDIIQALSLLLSDHQTLCRENLHQAYKHLAQLPKGAQGIVQSGLIPSLVRKLQKEEDHIQEIILDTLALCLQEDATEALESQAVPCLKEKLLSQNSEIRSKAARALIAISIPLDGKNQVWKNKVIPILVTLLSDTDEEVKANAAGALMHATVTTEGKYAALDANAIEPLLELLSTNPKTKLCLNATKALTMLAEAPEGRKLLLSHVPIFRYLLAHKNEAIQRAAEVAIKVIEWKP 374

[Close]

BLAST of Radial spoke head 14-like protein vs. Ensembl Mouse
Match: Rsph14 (radial spoke head homolog 14 (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1918486])

HSP 1 Score: 144.05 bits (362), Expect = 1.729e-40
Identity = 79/200 (39.50%), Postives = 120/200 (60.00%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHE 689
            MA  +IS   PPDID TKA +AYG RA+ +L  EL S DLLTRQKAL AL D +HD E++  ++N G + S++ LL+  D   R    EV Y+ + H VGR+  L++ +I  L  L SD + + R+ +++  +  ++   GA+ I+ + LIP L  K+Q+E D ++  IL+ L  CL  D  +AL++Q +  L   L  +
Sbjct:    1 MAHARISMYMPPDIDPTKAAIAYGCRALSKLNEELQSRDLLTRQKALVALCDLMHDPEYVYEAINIGCLESLKTLLQDDDNLVRIKTTEVLYIMATHYVGRVGFLKHDIIQALSLLLSDHQTLCRENLHQAYKHLAQLPKGAQGIVQSGLIPSLVRKLQKEEDHIQEIILDTLALCLQEDATEALESQAVPCLKEKLLSQ 200

[Close]

BLAST of Radial spoke head 14-like protein vs. Ensembl Mouse
Match: Rsph14 (radial spoke head homolog 14 (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1918486])

HSP 1 Score: 126.331 bits (316), Expect = 1.446e-34
Identity = 67/161 (41.61%), Postives = 100/161 (62.11%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEE 572
            MA  +IS   PPDID TKA +AYG RA+ +L  EL S DLLTRQKAL AL D +HD E++  ++N G + S++ LL+  D   R    EV Y+ + H VGR+  L++ +I  L  L SD + + R+ +++  +  ++   GA+ I+ + LIP L  K+Q+E
Sbjct:    1 MAHARISMYMPPDIDPTKAAIAYGCRALSKLNEELQSRDLLTRQKALVALCDLMHDPEYVYEAINIGCLESLKTLLQDDDNLVRIKTTEVLYIMATHYVGRVGFLKHDIIQALSLLLSDHQTLCRENLHQAYKHLAQLPKGAQGIVQSGLIPSLVRKLQKE 161

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BLAST of Radial spoke head 14-like protein vs. UniProt/SwissProt
Match: sp|Q9D3W1|RSP14_MOUSE (Radial spoke head 14 homolog OS=Mus musculus OX=10090 GN=Rsph14 PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 3.293e-66
Identity = 134/341 (39.30%), Postives = 215/341 (63.05%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLIN-DPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA  +IS   PPDID TKA +AYG RA+ +L  EL S DLLTRQKAL AL D +HD E++  ++N G + S++ LL+  D   R    EV Y+ + H VGR+  L++ +I  L  L SD + + R+ +++  +  ++   GA+ I+ + LIP L  K+Q+E D ++  IL+ L  CL  D  +AL++Q +  L   L  + +EI++KAA+ +I I++  +GK    K + +P L+ +L   +  VKA+AAGA+ +  + T GKYAA++  A+  LL L++ +P +++ +N+ KALT +AE PEGR+LLL H+ +F+  +    +A+ + A++A+ VI WKP
Sbjct:    1 MAHARISMYMPPDIDPTKAAIAYGCRALSKLNEELQSRDLLTRQKALVALCDLMHDPEYVYEAINIGCLESLKTLLQDDDNLVRIKTTEVLYIMATHYVGRVGFLKHDIIQALSLLLSDHQTLCRENLHQAYKHLAQLPKGAQGIVQSGLIPSLVRKLQKEEDHIQEIILDTLALCLQEDATEALESQAVPCLKEKLLSQNSEIRSKAARALIAISIPLDGKNQVWKNKVIPILVTLLSDTDEEVKANAAGALMHATVTTEGKYAALDANAIEPLLELLSTNPKTKLCLNATKALTMLAEAPEGRKLLLSHVPIFRYLLAHKNEAIQRAAEVAIKVIEWKP 341

[Close]

BLAST of Radial spoke head 14-like protein vs. UniProt/SwissProt
Match: sp|Q9UHP6|RSP14_HUMAN (Radial spoke head 14 homolog OS=Homo sapiens OX=9606 GN=RSPH14 PE=1 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 6.397e-51
Identity = 129/348 (37.07%), Postives = 203/348 (58.33%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKV--QEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTL------DAVAQHAKIAVDVITWKP 1109
            MA  Q S E P +I+ T+ T AYG RA+P+L  EL S DL TRQKAL AL D +HD E I  ++N G + +++ LL+  +   R    EV ++ + HSVGR A LE+ +++ L  L +D   + R  +YK      +   GA+ II+  LI  L  K+  + E +E + +IL+ L  CL  D  +AL +  + +L   L      I++KAA+ ++++++S EGK+     + +P L+ +LK     VK++AAGA+    +IT GKYAA+   A+  LL L++ P +  R+N+ KALT +AE PEGR+ L  H+  F+   ++T       +A+ + A+IA+ VI +KP
Sbjct:    1 MAHSQNSLELPININATQITTAYGHRALPKLKEELQSEDLQTRQKALMALCDLMHDPECIYKAMNIGCMENLKALLKDSNSMVRIKTTEVLHITASHSVGRYAFLEHDIVLALSFLLNDPSPVCRGNLYKAYMQLVQVPRGAQEIISKGLISSLVWKLQVEVEEEEFQEFILDTLVLCLQEDATEALGSNVVLVLKQKLLSANQNIRSKAARALLNVSISREGKKQVCHFDVIPILVHLLKDPVEHVKSNAAGALMFATVITEGKYAALEAQAIGLLLELLHSPMTIARLNATKALTMLAEAPEGRKALQTHVPTFRAMEVETYEKPQVAEALQRAARIAISVIEFKP 348

[Close]

BLAST of Radial spoke head 14-like protein vs. TrEMBL
Match: A0A1I8J1N9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 300.056 bits (767), Expect = 4.978e-96
Identity = 171/341 (50.15%), Postives = 239/341 (70.09%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELI-SPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA   IS   PP+ID TKAT+AYG  A+ RL REL  + ++LTRQKA+KAL DYLHDHEH+A++++ GIV S+++LL   DVTCR L+    YV S HSVGR A+L+ G I  L  LF D E I R+  +  +EM SE  FGA+ I+ A L+P L  KV+ E DE+K YIL+ LHFCL+++ +DAL+  G+++ T+LL      I+AKAA+ I+D+++  +GK+ A++  TVP LI +L    S V+A+AAGA++ IA+ T GKY A+N   +  LLPL+NDPNSEVRVN++K +TC+AE PEGR++L  H++  +    D +  V +HA+IAV VITW+P
Sbjct:    1 MATSVISANLPPNIDPTKATMAYGNGALRRLNRELQDTENMLTRQKAVKALCDYLHDHEHVAAAIHEGIVVSLKSLLRDTDVTCRQLSTMCFYVISQHSVGREAILDTGTIEPLSALFEDAEDICRRNAHMAIEMLSEVPFGADGIVQAALVPKLIEKVKSEHDEIKEYILDTLHFCLIVNTQDALKAHGMEVFTSLLASNLATIRAKAARDIMDLSVPLDGKDKAVEVGTVPALIGLLGDNASAVRANAAGALSFIAVTTQGKYTALNADCVNPLLPLVNDPNSEVRVNALKVVTCLAEAPEGRKILTGHVEQIRQLESDEITNVRKHAEIAVRVITWRP 341

[Close]

BLAST of Radial spoke head 14-like protein vs. TrEMBL
Match: A0A267ETD1 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006473g1 PE=4 SV=1)

HSP 1 Score: 299.671 bits (766), Expect = 5.606e-96
Identity = 171/341 (50.15%), Postives = 239/341 (70.09%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELI-SPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA   IS   PP+ID TKAT+AYG  A+ RL REL  + ++LTRQKA+KAL DYLHDHEH+A++++ GIV S+++LL   DVTCR L+    YV S HSVGR A+L+ G I  L  LF D E I R+  +  +EM SE  FGA+ I+ A L+P L  KV+ E DE+K YIL+ LHFCL+++ +DAL+  G+++ T+LL      I+AKAA+ I+D+++  +GK+ A++  TVP LI +L    S V+A+AAGA++ IA+ T GKY A+N   +  LLPL+NDPNSEVRVN++K +TC+AE PEGR++L  H++  +    D +  V +HA+IAV VITW+P
Sbjct:    3 MATSVISANLPPNIDPTKATMAYGNGALRRLNRELQDTENMLTRQKAVKALCDYLHDHEHVAAAIHEGIVVSLKSLLRDTDVTCRQLSTMCFYVISQHSVGREAILDTGTIEPLSALFEDAEDICRRNAHMAIEMLSEVPFGADGIVQAALVPKLIEKVKSEHDEIKEYILDTLHFCLIVNTQDALKAHGMEVFTSLLASNLATIRAKAARDIMDLSVPLDGKDKAVEVGTVPALIGLLGDNASAVRANAAGALSFIAVTTQGKYTALNADCVNPLLPLVNDPNSEVRVNALKVVTCLAEAPEGRKILTGHVEQIRQLESDEITNVRKHAEIAVRVITWRP 343

[Close]

BLAST of Radial spoke head 14-like protein vs. TrEMBL
Match: A0A1S3HML5 (radial spoke head 14 homolog OS=Lingula unguis OX=7574 GN=LOC106156564 PE=4 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 6.431e-94
Identity = 170/340 (50.00%), Postives = 235/340 (69.12%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA   IS + PP ID T+A LAYG RA+PRL RELI  +LLTRQ+A+++L DYLHD EHIA ++  GI  S++ LL+  DVT R  A E  Y+ + ++VGR A L+  +II L KLF+DKE I+RK  +K +EM SE   GAE I+ ANL+P L  K++ E DE+K  IL+ LHFC+ +D   AL+ + +++ T  L H    I+AKAA+ I+D+++  +GK  A++  +V  L+E+LK  ++ V+ASAAGAI  I I T GKY A+N  A+  L+ L++D NSEVR N++KA+TC+AE PEGR+ LL+HID  + R  D + AV +HA IAV VITWKP
Sbjct:    1 MAETVISAKMPPHIDVTRAPLAYGDRALPRLNRELIDLNLLTRQRAVRSLCDYLHDPEHIAEALRQGIPESLKGLLDDKDVTVRQKATECLYIIAGYAVGREAFLDQNIIIPLSKLFADKEDIVRKNAHKAMEMVSETMTGAEGIVEANLVPVLVEKLKTEHDEIKELILDTLHFCMRVDTAHALRAEAMQVFTNHLQHGTPVIRAKAARDIMDLSVPLDGKNKAVEVGSVASLVELLKDSDADVRASAAGAIMTITITTKGKYTALNCNAIEPLVKLVDDENSEVRANALKAITCLAEAPEGRQELLNHIDQIRKRTNDAIPAVVKHANIAVKVITWKP 340

[Close]

BLAST of Radial spoke head 14-like protein vs. TrEMBL
Match: C3YDA5 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_279978 PE=4 SV=1)

HSP 1 Score: 282.337 bits (721), Expect = 3.661e-89
Identity = 165/340 (48.53%), Postives = 229/340 (67.35%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA   IS + PPDID TKA LAYG RA+PRL REL   +L+TRQ+AL  L DYLHD EHIA ++  G+  S++ LL   D T R    EV YV + H+ GR A L+ G+I+ L KLF D   + RK  +  +EM SE  +GA  ++ A L+P L  K+++E++E+K  IL+ LHFCL ++   AL    +++ T LL H +  I+AKAA+ I+D+++  +GK  A + + +PPL+ +LK  ++ V++ AAGAIA I I T GKY A+  GA+PHL+ L+ D NSEVR+NS+KALT ++E PEGR+ LL+H+DL K RM D  +AV + A IAV VITWKP
Sbjct:    1 MAKTVISQKLPPDIDPTKAPLAYGDRALPRLNRELNDKELITRQRALMTLCDYLHDPEHIAEALRVGVAESLKKLLSDKDPTVRHKTTEVLYVIATHNKGRDAFLDYGIIVPLSKLFDDSVDLARKNAHMAMEMMSEIPYGAAGVVEAQLVPKLVGKLRDELNEIKELILDTLHFCLRVETMHALVAGAMEVFTELLGHNQAIIRAKAARDIMDLSVPLDGKNKACEVKALPPLVGLLKDGDTYVRSMAAGAIATITITTQGKYLALEAGAIPHLVELVEDKNSEVRLNSLKALTTLSEAPEGRKELLNHVDLIKKRMEDESEAVVKAATIAVRVITWKP 340

[Close]

BLAST of Radial spoke head 14-like protein vs. TrEMBL
Match: A0A4S2KYH1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_010469 PE=4 SV=1)

HSP 1 Score: 281.567 bits (719), Expect = 6.639e-89
Identity = 161/341 (47.21%), Postives = 225/341 (65.98%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKEL-IIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MA  +IS EPPP ID TKATLAYG  AI RL REL    LLT+Q+A+KAL DYLHDHEHIA ++N G+V S+  LLE  DV CR  A E   V   H +GR+A L++ ++ +   L    E+ I+R    K +E+ +    GA A++    IP L    ++E+DE++V +L+ L+ CL  D    L++ G+ + T LL HE  E++A+AA+ I+ ++++  GK+ A+  ET+P L+E+LK    TV+ASAAGA+A I    HG+Y  +N GA+PHLL L++D  S VRVN++K +TC++ETPEGRR+LLDH++       D   AVA+HAKIA  VITWKP
Sbjct:    1 MALARISLEPPPHIDPTKATLAYGRLAIKRLNRELHDAVLLTKQRAVKALCDYLHDHEHIAEAINEGVVTSLNVLLEDSDVPCRAYATECFVVLCQHELGRVAFLDHNILQSFLNLIKLSEVDIVRLNAQKAIELLTTSPAGALAVVENGYIPLLINCARDEVDEIRVVVLDTLNHCLSFDTAKGLESGGMPLFTQLLSHESPEVRARAAQNILRLSVNPTGKQEALDNETIPALVELLKDNSETVRASAAGALAFICTTNHGRYTTLNAGAIPHLLRLVDDETSRVRVNALKVITCLSETPEGRRILLDHLNTITSHEKDVNAAVAKHAKIAASVITWKP 341

[Close]

BLAST of Radial spoke head 14-like protein vs. Ensembl Cavefish
Match: rsph14 (radial spoke head 14 homolog [Source:NCBI gene;Acc:103021394])

HSP 1 Score: 167.162 bits (422), Expect = 4.506e-48
Identity = 119/343 (34.69%), Postives = 194/343 (56.56%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDV---KDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MAG +IS + PP ID  ++ LA+G RA+PRL  EL +P+L  RQ AL AL D +HD E    ++  G +  ++ LL+  D + RT   EV Y+ + HSVGR A+L   V+  L +L  +     R+  +  L+M S +  G   +++  L+P L +KV  E ++++  +L  L  C+ +D    + AL +  + +L   L H    I+ +A   +I I++  +GK    +EE +P L+++L  ++  +KA+AAG I N A+IT GK+ ++  GAL  LL L+   +  V  N+++ALTC+AE P  R  LL H+ L + R+      + + +  A+ VI+W P
Sbjct:    1 MAGSRISEQLPPLIDRNQSPLAFGQRALPRLAAELQNPELTVRQGALAALRDLIHDPERAYETIYHGCLERLKVLLKDEDSSIRTATTEVLYLLTAHSVGREAILREDVVGCLSELLDEPVDACRRNTHLVLKMMSGFPAGVACMVSLGLVPRLVMKVAAETEDIRELVLSTLGSCMRVDTQAAQAALASDVVTVLREQLTHSSTNIRREATAAMIAISVPPQGKVKVCEEEVLPVLVQLLSDEDLEIKANAAGTIMNTAVITKGKFQSLQAGALAPLLHLVECEHRAVCANALRALTCLAEVPRAREQLLQHLPLLQTRLNHPEPIIQRASATAIQVISWTP 343

[Close]

BLAST of Radial spoke head 14-like protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005642.1 (pep scaffold:Pmarinus_7.0:GL476474:164372:179435:1 gene:ENSPMAG00000005107.1 transcript:ENSPMAT00000005642.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 227.254 bits (578), Expect = 8.154e-72
Identity = 147/334 (44.01%), Postives = 201/334 (60.18%), Query Frame = 3

Query:  108 SFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            S + PPD+D TKATLA+G RA+PRL REL  P+LLTRQ+AL AL D +H+ E    +V  G +  +R+LL   D T R    E   +   HS+ R   L   +I+ L  LF D   I R   +KTLEM SE   GA  I+ A L+P L  K++ E++E+   IL+ L+FCL +D   AL+   + +L  LL HE   I+AKAA+  ++I +S EGK  A  ++ VP L+ +L    + V+ +AAGAI  IA+ T GKYAA+   A+  L+ L+ D  SEVR+N+IKA+T +AE PEGR  LL  + L  G   D   AVA    IA+ VITW+P
Sbjct:   12 SVQLPPDVDPTKATLAFGDRALPRLCRELQGPELLTRQRALMALCDLVHEPEMAYEAVRVGCLECLRSLLVDEDATVRCKTTEALSIMVSHSIVRTMFLSMDLILPLSHLFDDDVDICRLNTHKTLEMLSEIPHGALGIVEAGLVPCLVKKLRSELNEICELILDTLNFCLAVDTMQALEVGAVDVLLTLLLHESERIRAKAARATMNITVSLEGKNQACDKQLVPVLVGLLSDSSTDVRGNAAGAIMTIAVTTQGKYAALGAEAVHPLVELVTDGRSEVRLNAIKAITALAEAPEGREALLPSVSLLGGCRSDASAAVANAVHIALQVITWRP 345

[Close]

BLAST of Radial spoke head 14-like protein vs. Ensembl Nematostella
Match: EDO46598 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPM5])

HSP 1 Score: 241.121 bits (614), Expect = 5.058e-77
Identity = 148/341 (43.40%), Postives = 216/341 (63.34%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLD-TLDAVAQHAKIAVDVITWKP 1109
            MA   IS + PPDID TKA LAYG RA+P+L REL S DLLTRQKAL +L D +H+ E+I   +  GI  S+++LL+  D T R    EV ++ + H+VGR A LE+ +I+ L +LF+D+    R+  ++ +EM S    G + ++ ANL+P L  K+ EE DE+K  IL+ LH+C+ ++ K  L + G++  T LL H     + KAA+ I+D++   EGK+ A+    VPPL+ +L+   + V+A AAGAI    I+   KYAAI   A+P+L+  ++DP S+VR+NS+KA+T +AE P GR  LL  +   +  + D    AV + A+IAV VITWKP
Sbjct:    1 MATTLISVKLPPDIDPTKARLAYGDRALPKLNRELNSDDLLTRQKALMSLCDAMHNPEYICEGIRVGIAASLKSLLKDADATVRHKTTEVLFIIAGHAVGRDAFLEHSIILPLSELFNDQVYQARRNAHQAIEMCSHTSPGTDGVVEANLLPILVGKLLEEQDEIKELILDTLHYCMRVETKQVLDSNGMEAFTKLLDHMDPSTRCKAARDIMDLSFPLEGKDRAVDVGAVPPLVSLLRDNSAEVRAQAAGAIMTQTIVPSSKYAAIEAEAIPNLVSQLSDPVSDVRLNSLKAITTLAEAPIGRTTLLKSVKEVEKLVHDHDSPAVRKAAQIAVKVITWKP 341

[Close]

BLAST of Radial spoke head 14-like protein vs. Ensembl Medaka
Match: ENSORLT00000034911.1 (radial spoke head 14 homolog [Source:NCBI gene;Acc:111947860])

HSP 1 Score: 99.7525 bits (247), Expect = 4.358e-23
Identity = 100/342 (29.24%), Postives = 166/342 (48.54%), Query Frame = 3

Query:  126 DIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQ----EEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLIND--PNSEVR-------VNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHA-KIAVDVITWKP 1109
            D D   A +A+G  A+P L  +L  P    R +AL ALID L        ++  G +  ++ LLE  D   R    ++ +V + H+ GR ALL    +  L +L  D     R+ V+  L   S +  G  +++   L+P L  K+Q    EE DE++V  L  L  C   D + AL++  + +L   L +    I   AA  ++ +++   GK     ++ +P +  +L++++   + +A G I   A+ T GK   ++   LP LL L  D   + ++R       +NS++ LT +AETPEGRRLL D + L +    +  D   Q   + A+ V+T+ P
Sbjct:    4 DSDAPPAPVAFGRWAVPLLAEQLRLPGRDVRLRALAALIDVLRQPLRGPEALTEGFLQQLKVLLEDEDPCVRAKVCQLLHVLAAHTCGRQALLAASFLPPLSQLLEDPP--CRRTVFLLLSRLSLHSSGRVSLL--TLLPKLMKKLQEEEEEEEDEVQVLFLSLLRCCFQADPEAALRSDAVALLAPKLSNGALNICKAAAAAMMALSVPMGGKVEVCDQKVLPAVAALLQEEDEEAQLNATGVIMFAAVTTAGKLQCLHLNVLPVLLKLACDRHEDEDMRRRRKPLVMNSVRGLTMLAETPEGRRLLQDQLPLLRRTSEEEEDVDIQRVFETAIQVVTFTP 341

[Close]

BLAST of Radial spoke head 14-like protein vs. Planmine SMEST
Match: SMESG000077061.1 (SMESG000077061.1)

HSP 1 Score: 623.624 bits (1607), Expect = 0.000e+0
Identity = 338/340 (99.41%), Postives = 340/340 (100.00%), Query Frame = 3

Query:   90 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEXXXXXXXXXXXXXXXXMSHEGKEMAIKEETVPPLIEMLKQQESTVKXXXXXXXXXXXXXTHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 1109
            MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAE+IINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEKNEIKAKAAKCIIDIAMSHEGKEMAIKEETVPPLIEMLKQ+ESTVKASAAGAIANIAIITHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP
Sbjct:    1 MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKALIDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVGRMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAESIINANLIPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHEKNEIKAKAAKCIIDIAMSHEGKEMAIKEETVPPLIEMLKQKESTVKASAAGAIANIAIITHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAETPEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP 340

The following BLAST results are available for this feature:

BLAST of Radial spoke head 14-like protein vs. Ensembl Human
BLAST of Radial spoke head 14-like protein vs. Ensembl Celegans
BLAST of Radial spoke head 14-like protein vs. Ensembl Fly
BLAST of Radial spoke head 14-like protein vs. Ensembl Zebrafish
BLAST of Radial spoke head 14-like protein vs. Ensembl Xenopus
BLAST of Radial spoke head 14-like protein vs. Ensembl Mouse
BLAST of Radial spoke head 14-like protein vs. UniProt/SwissProt
BLAST of Radial spoke head 14-like protein vs. TrEMBL
BLAST of Radial spoke head 14-like protein vs. Ensembl Cavefish
BLAST of Radial spoke head 14-like protein vs. Ensembl Sea Lamprey
BLAST of Radial spoke head 14-like protein vs. Ensembl Yeast
BLAST of Radial spoke head 14-like protein vs. Ensembl Nematostella
BLAST of Radial spoke head 14-like protein vs. Ensembl Medaka
BLAST of Radial spoke head 14-like protein vs. Planmine SMEST

BLAST of Radial spoke head 14-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
RSPH14	1.332e-51	37.07	radial spoke head 14 homolog [Source:HGNC Symbol;A...	[more]
RSPH14	3.087e-19	45.54	radial spoke head 14 homolog [Source:HGNC Symbol;A...	[more]

BLAST of Radial spoke head 14-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Radial spoke head 14-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Radial spoke head 14-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
rsph14	6.312e-51	35.88	radial spoke head 14 homolog [Source:ZFIN;Acc:ZDB-...	[more]
rsph14	5.198e-33	36.68	radial spoke head 14 homolog [Source:ZFIN;Acc:ZDB-...	[more]

BLAST of Radial spoke head 14-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
RSPH14	7.176e-75	44.71	radial spoke head 14 homolog (Chlamydomonas) [Sour...	[more]

BLAST of Radial spoke head 14-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Rsph14	4.707e-67	39.30	radial spoke head homolog 14 (Chlamydomonas) [Sour...	[more]
Rsph14	4.707e-67	39.30	radial spoke head homolog 14 (Chlamydomonas) [Sour...	[more]
Rsph14	1.501e-66	39.30	radial spoke head homolog 14 (Chlamydomonas) [Sour...	[more]
Rsph14	1.729e-40	39.50	radial spoke head homolog 14 (Chlamydomonas) [Sour...	[more]
Rsph14	1.446e-34	41.61	radial spoke head homolog 14 (Chlamydomonas) [Sour...	[more]

BLAST of Radial spoke head 14-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2


Match Name	E-value	Identity	Description
sp\|Q9D3W1\|RSP14_MOUSE	3.293e-66	39.30	Radial spoke head 14 homolog OS=Mus musculus OX=10...	[more]
sp\|Q9UHP6\|RSP14_HUMAN	6.397e-51	37.07	Radial spoke head 14 homolog OS=Homo sapiens OX=96...	[more]

BLAST of Radial spoke head 14-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1I8J1N9	4.978e-96	50.15	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A267ETD1	5.606e-96	50.15	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A1S3HML5	6.431e-94	50.00	radial spoke head 14 homolog OS=Lingula unguis OX=...	[more]
C3YDA5	3.661e-89	48.53	Uncharacterized protein OS=Branchiostoma floridae ...	[more]
A0A4S2KYH1	6.639e-89	47.21	Uncharacterized protein OS=Opisthorchis felineus O...	[more]

BLAST of Radial spoke head 14-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rsph14	4.506e-48	34.69	radial spoke head 14 homolog [Source:NCBI gene;Acc...	[more]

BLAST of Radial spoke head 14-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000005642.1	8.154e-72	44.01	pep scaffold:Pmarinus_7.0:GL476474:164372:179435:1...	[more]

BLAST of Radial spoke head 14-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Radial spoke head 14-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO46598	5.058e-77	43.40	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Radial spoke head 14-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSORLT00000034911.1	4.358e-23	29.24	radial spoke head 14 homolog [Source:NCBI gene;Acc...	[more]

BLAST of Radial spoke head 14-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000077061.1	0.000e+0	99.41	SMESG000077061.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30009188 ID=SMED30009188|Name=Radial spoke head 14-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1180bp
ATGAAAACACTTTTATATTAAAATTATTAGGTCTTACCATTTTCAGATTA
ATTTAATTTTCTAAACTTTTAAGATTTTACCAATTTTTAATGGCTGGTGT
ACAAATATCATTTGAACCTCCACCAGATATTGATTGTACCAAAGCAACAT
TAGCTTATGGTTTAAGAGCCATTCCAAGACTAGGTAGAGAATTGATCAGT
CCAGATTTATTAACTAGACAAAAAGCCCTTAAAGCTCTTATTGATTATTT
ACATGATCATGAGCATATTGCATCTTCTGTTAATGGAGGTATTGTTCCAT
CAATTAGAAATCTACTTGAAGCGTGTGATGTTACTTGTAGAACCTTGGCA
GTTGAAGTTTGTTATGTATACAGCATGCATTCAGTTGGAAGAATGGCACT
GTTAGAAAATGGTGTCATAATTACACTTGGAAAATTGTTTTCTGATAAAG
AATTGATTATACGAAAATTGGTTTATAAAACTTTAGAAATGACCTCGGAA
TATTGGTTCGGCGCCGAGGCTATTATCAATGCCAATTTGATACCAGATTT
ATTTATTAAAGTTCAAGAAGAAATTGATGAATTAAAAGTATACATTTTGG
AATGTCTACATTTCTGTCTGTTACTCGATGTCAAGGATGCTCTACAAAAT
CAGGGCATCAAAATTTTAACTGCGCTTCTTTTTCATGAAAAAAATGAAAT
CAAAGCTAAAGCAGCAAAATGTATCATCGATATAGCGATGAGTCACGAAG
GAAAAGAAATGGCCATCAAAGAAGAAACGGTTCCTCCGTTGATTGAAATG
CTAAAACAACAGGAAAGCACCGTAAAGGCATCCGCTGCTGGTGCCATAGC
AAATATTGCTATAATTACCCATGGAAAGTATGCCGCTATCAATTTTGGTG
CTCTGCCTCATTTGTTGCCTTTGATAAATGATCCGAACAGCGAAGTGAGA
GTCAATTCCATCAAAGCGTTGACATGTATTGCTGAGACACCGGAAGGACG
CAGACTACTCTTAGATCACATAGATCTATTTAAGGGCCGAATGCTGGATA
CCCTGGATGCAGTGGCACAGCATGCTAAAATAGCAGTCGATGTCATCACA
TGGAAGCCTTAATGATGTAATAATATATTAATAGTTCATTTCTGTTTTTG
AGTAAAATAAGGTTCTTTATTGATTTGGAA

protein sequence of SMED30009188-orf-1

>SMED30009188-orf-1 ID=SMED30009188-orf-1|Name=SMED30009188-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=341bp
MAGVQISFEPPPDIDCTKATLAYGLRAIPRLGRELISPDLLTRQKALKAL
IDYLHDHEHIASSVNGGIVPSIRNLLEACDVTCRTLAVEVCYVYSMHSVG
RMALLENGVIITLGKLFSDKELIIRKLVYKTLEMTSEYWFGAEAIINANL
IPDLFIKVQEEIDELKVYILECLHFCLLLDVKDALQNQGIKILTALLFHE
KNEIKAKAAKCIIDIAMSHEGKEMAIKEETVPPLIEMLKQQESTVKASAA
GAIANIAIITHGKYAAINFGALPHLLPLINDPNSEVRVNSIKALTCIAET
PEGRRLLLDHIDLFKGRMLDTLDAVAQHAKIAVDVITWKP*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000099	neuron
PLANA:0000106	ciliated epithelial cell
PLANA:0000418	head
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR032682	Cnd1_C
IPR042856	RSP14
IPR000225	Armadillo
IPR000357	HEAT
IPR011989	ARM-like
IPR021133	HEAT_type_2
IPR016024	ARM-type_fold

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding
GO:0005488	binding
GO:0003674	molecular_function

Vocabulary: **cellular component**
Term	Definition
GO:0005575	cellular_component

Vocabulary: **biological process**
Term	Definition
GO:0008150	biological_process

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000225	Armadillo	SMART	SM00185	arm_5	coord: 218..258 e-value: 0.062 score: 22.4 coord: 259..299 e-value: 45.0 score: 8.2
IPR000225	Armadillo	PROSITE	PS50176	ARM_REPEAT	coord: 229..271 score: 8.522
IPR011989	Armadillo-like helical	GENE3D	G3DSA:1.25.10.10		coord: 15..334 e-value: 7.8E-32 score: 112.4
IPR032682	Condensin complex subunit 1, C-terminal	PFAM	PF12717	Cnd1	coord: 224..304 e-value: 3.9E-6 score: 27.0
None	No IPR available	PANTHER	PTHR15599:SF1	RADIAL SPOKE HEAD 14 HOMOLOG	coord: 6..327
IPR042856	Radial spoke protein 14	PANTHER	PTHR15599	RTDR1	coord: 6..327
IPR021133	HEAT, type 2	PROSITE	PS50077	HEAT_REPEAT	coord: 271..306 score: 9.196
IPR016024	Armadillo-type fold	SUPERFAMILY	SSF48371	ARM repeat	coord: 26..312

Main menu

Radial spoke head 14-like protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Owlery

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