SMED30009147

Overview
NameSMED30009147
Smed IDSMED30009147
Length (bp)803
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30009147 (SMED30009147) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30009147 (SMED30009147) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30009147 (SMED30009147) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30009147 (SMED30009147) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30009147

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30009147SMESG000020794.1 Contig186newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30009147SMESG000020794.1 Contig186uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
nervous systemSMED30009147 dd_Smed_v4_782_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30009147 dd_Smed_v4_782_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30009147 dd_Smed_v4_782_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30009147 dd_Smed_v4_782_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30009147 dd_Smed_v4_782_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30009147SMESG000056234.1 OX_Smed_1.0.17381ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30009147 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006924:27886..28733 +3832
v31.013445:18627..19470 +3655
Homology
[Close]
BLAST of SMED30009147 vs. TrEMBL
Match: A0A1D1VRY9 (Uncharacterized protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_13057-1 PE=4 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 3.402e-7
Identity = 22/47 (46.81%), Postives = 30/47 (63.83%), Query Frame = 3 
Query:   69 PRGKHLVRYWSPNIFLYTTAAGMLVCYMTDWKAVNQYIPFYGSKYSE 209
            PR   L + W+P++  +  A G+L  Y+TDWK + QYIP YG KY E
Sbjct:   10 PRYMELAKAWTPSLMAFGAAGGLLGLYLTDWKVITQYIPLYGGKYKE 56
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BLAST of SMED30009147 vs. TrEMBL
Match: A0A158NIE0 (Uncharacterized protein OS=Atta cephalotes OX=12957 GN=105620412 PE=4 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.993e-6
Identity = 24/48 (50.00%), Postives = 31/48 (64.58%), Query Frame = 3 
Query:   66 TPRGKHLVRYWSPNIFLYTTAAGMLVCYMTDWKAVNQYIPFYGSKYSE 209
            T +   L   W+P+I  Y T A + + Y TDWKAV +YIPFYGSK+ E
Sbjct:    6 TRKNIELASKWTPSIVTYGTGAWLSLLYFTDWKAVVRYIPFYGSKFQE 53
The following BLAST results are available for this feature:
BLAST of SMED30009147 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A1D1VRY93.402e-746.81Uncharacterized protein OS=Ramazzottius varieornat... [more]
A0A158NIE07.993e-650.00Uncharacterized protein OS=Atta cephalotes OX=1295... [more]
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BLAST of SMED30009147 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30009147 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30009147 ID=SMED30009147|Name=SMED30009147|organism=Schmidtea mediterranea sexual|type=transcript|length=803bp
NNNTNTNCAANATTGTTTAACTGCATACAATTTTCAATTTCATGGGTATT
TGGAAAATTATTAAAACTCCAAGAGGCAAGCATCTTGTTAGATATTGGTC
TCCTAATATTTTCCTTTATACTACCGCTGCAGGAATGCTAGTTTGCTACA
TGACTGATTGGAAAGCTGTTAATCAGTATATACCTTTTTATGGATCAAAG
TATAGCGAAAAACCCATTCACTAATTAGAATACCGTCACTATTTTAGCTT
TAGGAAAAGTGGTTCAATGATAATATTGTAGTATCATTAGATGCCTATGT
TCAAATGTGTTTAATAGCTTGTTATTTGAAATTAATTTTATCGAAACAAA
TTTGATAAAAATTTATTTGTAAAGTTAATTTCTATGTTTGGTTATTTTTT
AACAATTACCAAAATTTTATTTGTAATTTTGTTCGTAAAAGGGGCTTTAA
GAATTTCAANNAANNNANNNNNNNAGGTTACTAAACATTTATATTGTATG
CTAATACGTAATTGTTTAATTTTTGCTATTTTATTAATTTAATTAAAGTA
TAATAGCCAAATTATTTATATTAAGTTCATATGAGTGACAAAGCACTAAA
AACAGTCACTATCATACCCCACTTTATTCAACGGCACGAAAGTTTTTAAA
AAACTGATTGAGTATTTCATGTAATTATATAATATATGCGTAATTAATTC
GTGCTTTAATAAAACTTAAAAGTATTTCATAACAAATTTTAGTGTTATCA
ATTACTGCGGTAAATTTTTATTCATGTGTGCCCTCGATAAATATTATTTC
TTT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0000418head
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR029027Single_a-helix_sf
IPR015089UQCR
Vocabulary: molecular function
TermDefinition
GO:0008121ubiquinol-cytochrome-c reductase activity
GO:0009055electron carrier activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015089Cytochrome b-c1 complex subunit 10PFAMPF08997UCR_6-4kDcoord: 11..56
e-value: 2.4E-14
score: 53.0
IPR015089Cytochrome b-c1 complex subunit 10PANTHERPTHR15420UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 6.4 KD PROTEINcoord: 9..56
NoneNo IPR availableGENE3DG3DSA:1.20.5.220coord: 3..57
e-value: 4.1E-15
score: 57.1
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
IPR029027Single alpha-helix domain superfamilySUPERFAMILYSSF81518Subunit XI (6.4 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)coord: 10..54